<?xml version="1.0" encoding="UTF-8"?>
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<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPB5</accession>
    <name>A0A8S1JPB5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPB5"/>
    <dbReference type="OMA" id="IHYRKIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="167"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="363" mass="43834" checksum="846A16587494B88A" modified="2022-10-12" version="1">MQLTKENIEFYIQRRKQYCLDFLQQTNQFKPKLAQLQKRLIATKQGIDGPIKSISHQQKIKKVNKVLASLSKSKEVQNKIDKEMIRCQCQYELDEVPNCNFNYSQTCNKIQQAMIPLIKNLQIQIKGKQNEIQSDQDCADLYIKKGEFQQMQHKSKRLQQIIEKLHIMELQQEEKVYRESIKKLEKFDQDEEYDETLPYQTLLSKRRLEAKVLIDEERKQKKQIENERIKENIEEQKQKYHLLDNKIKIHYRKIGIQKGKLESMSDFREQMNDQKTITQINVDSQNSVKKQLLKSILKADKRFSLTLFSTQLNNSNIPHQYQQYQQYIKKYQKQYWKEYILAFQQDRKTISYIRIIIRKQIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JT08</accession>
    <name>A0A8S1JT08_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0100054</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JT08"/>
    <dbReference type="OMA" id="EANNEKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1105"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1150"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="233"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="742"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8045842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1205" mass="142618" checksum="29B8D0DF223A89C1" modified="2022-10-12" version="1">MQFLQRSNNSVKSIESQIFGRRIQEPNVAVMLCKHQPNQKHHSPESFLDQFVNLKLMEPQKILQKQKPLQGLQGGKGIANTVQLLKPVQSSISVKKQTKRTISPKKTKAQTVDCIQMNKPKSQQKRREQMGLGELNEKQIKQRMKKIKQKEKIIEQPLKKKPYEPNIKLQSYIIQKKALINNNYMQQQLMVEIEKQRKLENLAQLNQSVKDIFKKCKTQENSNLQPSKGIIKRRKVEAQKENAQQNLRQVKNKTKQLQQKFDDISHRYQQIQNVRQNDMDNIEQCIFEMDEEDDQYSAVSDNQMIQLALHELQKIKPTQYLIDKVKYIIEHQNNIELDFITEQDYQVIQYHLMNQAAIKLQSLWRGVYIRKQILNELQNMMDEEYENKSYEDPSNIYQRNQFQQEFVGNISNSKGDQISNTSLINKIGNNSLQDLNINQQEQININEQSKQHFYSDQDQQFDIKLSDQISELIMIQQPNSPIEQNENFSLQFGQMDKRNCSEQIESLVRIEDEKTNSLSQSVIQQIQQELESWNSQIDTIFQQSNDDGDTISKVKQQISQTVIKIVNSHLKKSREIKLLNESLNDEKIRNQYEANNEKHFNDTFKRLKKSKEFSAEKLLHSSREIQQNSNIMNLESQLKMKEIELLDMREQAINLRYFSELKKIKQDSNKKQELDLWLEKEKEDLRTTKQAIEISRIREAQTIQKIQRDLHIASKIDENNPKIQNFKKFIDEQLLETNSLFNDSKIIENQIIEQEIENQEQNEKNILSEITTNMNETYNHINYITNSILDQVVNNLSQELFSNKTEFNLIMSIFINQQSNYQSKTSTQYIKDYIKNLFDFILLNYSEDLIKNLNIPYGFRPLKRIQLMHGYDADNDIQNQDLKSTQIAFPIDQVVFTKFEQQRIELNQNRNIYQIDNEYYFENIHNKAIFDACNEALNYQRQYYLNQGLPYPWEQLEYQVIVQKDKLPSILRNMEDKIINWSKTLGGFLPIENTYQISQEKQEVLEEKNSQLTLSQLNRFEKKTFLDSQQQEQIHENISSIRDERIYKLLIQDIKENEFRWYLEEDDRAEFLMELRNSLGRTLIMNIYYYKQLKQLQEEMISDTVLLVAIIGAASILVVIGIFCIWRKLKIRTINNLNNKPMPKAYVEIPDKTDRQARPPTNPQQCSPKGDEDSIAIKRKVKNFSSAVVNKKKNLTIKIPTKEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTD4</accession>
    <name>A0A8S1JTD4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0100100</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTD4"/>
    <dbReference type="OMA" id="QIEGWVM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000178">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040724">
        <property type="entry name" value="PheRS_DBD1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003210">
        <property type="entry name" value="PRK04172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18552">
        <property type="entry name" value="PheRS_DBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="247"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8045934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="518" mass="59375" checksum="8AA7549A5363FFC4" modified="2022-10-12" version="1">MDQKHDHANQILSLLENNESINSLQISQTTGVNHQELVGYLLQLEAANYIITEGLKKDQLVLTEEGNTILNSQSGEYEIYQKVPEGGIQMVDLQKQFGIEQAPKQKQEQGEKKEKQAEDPKKKLFDLGFGNAKKMGYIKMDKGIISKVANDLPDKIKQELQQFNEIKLGSPQAKLLIERKLASISTIKYFEVKKGVKYSPKFREQFAALTTEQLLNGEWKDCDYKSINLNAEGEKISMGNLHPLLKVRKQFSQILIGLGFEEMATNQFVESSFWNFDALFQPQKHPARDAHDTFFCSDPELSEEVDTQLRDKVKSMHQTGGVGSIGYQYEWSHEEARKNILRTHTTAVSSKMLYRIAQQYKEKGYFPKKYFSIDKVFRNETLDATHLAEFHQIEGVVIDKNASLGQLMGIIREFFRQIGINKLWFKPTYNPYTEPSMEIYGYHPILKKKVEIGNSGVFRPEMLAPLGIPEDVNVIAWGLGLERPTMIYYDIRDIRTMVGPEVKVETVRSNAFCVFESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JUC0</accession>
    <name>A0A8S1JUC0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JUC0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="34" mass="4307" checksum="53EF3541DEB7C223" modified="2022-10-12" version="1">MEKLQQFVGQDYDSKRIYKYVQQILQMEIDQRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZ60</accession>
    <name>A0A8S1JZ60_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0120257</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZ60"/>
    <dbReference type="OMA" id="SAWETSD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF48">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="2">
        <location>
            <begin position="715"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8047959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1019" mass="120090" checksum="A2FC6586DF50CF42" modified="2022-10-12" version="1">MNQINQMNFLQPFNYCMYPYRAPYYYNLYADNIQEQNSSTATYKSEVISWLSTLEQSELYKLFQIKGQIKTFPIMKMYIYDKLNNPSNYALLQQATINLESKIDDQFMMKFKALHPKAEDLYTKLNIVDDENFMDTIFVAEPHLENLNSFLDLLNDLSDKYFLSTSPKAIIEKAEDPIWFHQNSFHSCAVWIIKEFEKNISFHYNLHREKKKKQRLFRQPIYKNTSNELKEFFIQNLANNKERLTYYFQEIYSEIKQQPDHLENKFYENIFSGTAIKLLKSQVELLLNFQQKCHTDTNIINSMLITSMNDLVDQKTYILKKFYKIVQQLFQEHLEQELFKTEISDKKQKKDKKKKKKKYQKMQTFKGLDPIELNKRLSSKNLQESNILRFQRSYSQSNLTYTYVTPPNTPSAWETSDDQNEYYKVSNTQQQLSKCQSSNNPKINLIQIEQPLISQQFHTNLENPTNDDFVDVGQSITINILEIANISLNDENQFKEQYLKDKKRIKKKQKGFQKSIPESRDEQLQLQSPQSEITRFSIETQAQTIKSHCSNRTSSTSEEEEGKQQLNIIKNQIKIRKNSNENKKNIFKLINSFKMNQFEDQQINQDINSASNESEQISQKTLEDDIVTKNQKKNQQFDIKKNVSILQQNSEKSLKEQLENVQQKGKQKLIEQINLDILDFTDNIMSEYEDMLPFRFLAFERVKLVIQKVFLGIPDGMIMLFGSCATGLALIDSDIDIGINGLEVYNRNMLKVHFDNLFFEFSRKKWVVKANPIFNSSVPIIKLEIDPQINIFEYEGRNLDEQQIQQWKKLKQKLKSGIKVDISFNFNGNAIYSTHLGSITTDLVKKWMEEYPSLQQIVLIFKSMIKKLKLSESYTGGLSSYSLIIMVYSYMREQRITSNLIGEQFIDMLNFYVNCFDPSSTGIGLLADINNPNSSYFFNLQDYCLPMLPITIFNPYNRKLLTHSCVQINKIFDFFKVILKELEIKKDFYCNYVVLGKKKQQKLNKSLENFIISILEQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0L7</accession>
    <name>A0A8S1K0L7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="EFITHNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="7129" mass="794931" checksum="7EF2EF91FC9A3723" modified="2022-10-12" version="1">[encoded data, 7129 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3R8</accession>
    <name>A0A8S1K3R8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0130411</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3R8"/>
    <dbReference type="OMA" id="CIWFLIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="173"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8049331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="888" mass="105186" checksum="98C633BF0583BDB5" modified="2022-10-12" version="1">MNNYQEKIGLFSINTVSPKEWQLNADLDVELQAKPIETDNLDLLLSPLTKVSNQAKFFLDYEKRSHVSQLSILGNNMMAYFNLKKFLQKLSRNRENFKKLTSKHLDVINDQASDQTVLLNSNKNKPKLKIFDFPVLLSQLSKKNSLLTTWKRSILEDLNKIPLIHPESKKKLIWDYFMTALRIVLMILLPLEIAFQPGIVFEKAQIFTSVTILLTILDMIVRLNTVYYEDGKAINDRWKIFELKIQDCIIFDVIAIFSLTVNFGVVLRSILPSYSLQFKYVYEVINKSQESSYLSKPIQGLMKLIWFILTLLTILHIFSCIWFLISQNTYGDNWVKNKEIDESEWATQYIEALYFSTVTMLTIGYGDSVPQNQIEKLVSILFILIACLWYSYAVNTIGQIINELTYNSEKRRQRIRVINGYMNKRKVPYSLQFKIREYLNYRWKEEQESDITQEDKLINELSNELKQELELLGRKNFIEKSQLLQHFSNEFKRSVSSHIKRVIIQPHNTFSMKEDPCLCYLESGQLQFLMTEIKEGQFIQEYEYATDTQTSIPYKAIGYKDIFNKILKAHQIDHEKFSQLKLKLSSEQYGYLYECKWCSTHGHSTNYCPLVSYKYDREGIIKKHCFPKQNDRVFIIRNRVRNYFKSLSEMELIQEYVAYFQSEKQQIIQDQLKKQLSDSQVNMQIQRKQSIFEDEFQEFGIKRKSIHENNGRSPIQKLGNSNIGQSNFQFLGVIKPKQSELEKSFMLKTIQPNMIEEEQIQNINALYLKSKKDGYMNQDLEMLYLKYNCLPVKDFDHIKSFQHYFPEFNVEQVIKKKREVYKSYFKELIRYMLYPFLYVMKFNKIAHKRKLTGNFQQVGNIMKFKKALLNKKRRQTSVFPNGQPLIHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4E2</accession>
    <name>A0A8S1K4E2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4E2"/>
    <dbReference type="OMA" id="CRESALN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="964"/>
            <end position="986"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="986" mass="116769" checksum="D77E2832371D8EAA" modified="2022-10-12" version="1">MKKIAKKQNNRESELQQIEEIKIVEIYESMNDPDIEQSSVIIKLEDLLLESPQGVLLSFVSFYLKLVGMDHCNQYLKGLSLAKVNLEEIINKLESLQLENQSNLIDEQQCQLIENNKLLRQFFDTLFSTQTQLPFQCEGFFKYTYNWLTIISQTKFRTARQAALEVIDLIINSTSNFLQFIKNEQELKEVTKFINNIFEYLVTNRTQDVQTLIKKKAIQILFHSFQNSDIPTDPYYSYLGCLFLSENHECRESALNELEKILKSLIESKKSLQPIIDFLQEEQEAVMSLIFDPNESVQLKLINFLKLFSKNTNVVSNFFKTKTCQAFIRILFAKNLRIRYESSFLLHLFEKPIKSVYKLTEFYLTYAPFEIQSYEECQNFIFSFVHSHSFVCLPEQYSQFFEGEQQEQFQLMGFYFYASILSYQHTQIKFWWLEDKDEEIKFTDQFTSYFLTKAKISIDLVDENLLFPYLHIYQNLNSKLIPLKDMKAIAISVQKIFQKSQNIGIINQVCKMMFIYQQQQQDSEVVKECIKELVKYGIKIYKEKLNFLNIQKIECLIKQNLVFPKQILNPISSIFENKDEKSIIINANLLASSLQNQIRNLLSSNKPQENDHEINYKEIRDLFFNYCFQILENKNKDQYHITILQLVINNLIYTNNFKLQRLNLQYYISSQTYQLLYIHFHNFVLSELSRQVKLEKEKQNDFKRRKSRTLIDNLPQISNIQMELFNNSIKLINQCPSILINKEFGIQFLSYLFELIKSSCYFSDLKPTILTSISQSLQKVIENDQNLKKEDFWKFIIDYSSIQYQSQSEENFNEFVKLCINSYIASVNKSNVNEKRMKFINHCAEMIQIGFQDVSNIFLFGLLTHIFYKPTKLLKTLQIDKLTYQKFYAFYESKSSEYHQSNKQEEEKKEILKSEQQLRNRLKQQTGLTTKEINKIVNRVSKSPQKLNVVSKCIVDNSQVSNSKEKFKQDSDEELEIPFSKQKATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4T3</accession>
    <name>A0A8S1K4T3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0140464</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4T3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035831124" description="Trichocyst matrix protein" evidence="3">
        <location>
            <begin position="17"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8050358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="39551" checksum="A8BBACD0F1BC0762" modified="2022-10-12" version="1">MKFTICFLLGLVTLNAVFVKKPSDPNAVVFAELEEIEEHALGRKLLDTIALQMKNQAPLADIARMLQELRENLLLQQQQAELKHAADEADCAAEIAGYNRRIDYASNEISESTTEITALSAQVQQLESEIENIQVQLGILNDQEETLRTQRAKDAEAFTQRIKSTADVVEALNVVAAKLSAIQPEQDPKAVFLELHNMGKSNPIAALVSIASAFSKERLQQTQDKIAELRQSIEQSAVDDQEAEVQAQIDYQNMLAQFADQRKNFQNALKDNEAKLTQTQNALGAQKKRKEDAGRELATATAGKTQKENDCEALRTQYARDSEQRTKEIGIIRQVEEILATKLEGASGYLKNRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K5M0</accession>
    <name>A0A8S1K5M0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K5M0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="26309" checksum="2010EBD299BD4E66" modified="2022-10-12" version="1">MVFILSKINIQILIQRRNNLYQCFQKHQKNIAKIIYHFTQQSKDVWSDAELLYHPKIPLNFYNGQCSLYQSQIFNRFRTYYILISAFGRYLTYYSIKKLYNYQDRKFIGILFYLGLLGITLCSMRSVQSQYQRLLISIDLLPLGRKLLIQQPSLYGYCISKQIDIATISRPKDVYQKSIQKMEHVFPVIMDTQLVYLHRDPDYIVNKGIFACSNECQIYQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6A3</accession>
    <name>A0A8S1K6A3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0130413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6A3"/>
    <dbReference type="OMA" id="NLENPCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="140"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="542" mass="64210" checksum="6106311B25F534B1" modified="2022-10-12" version="1">MKRFLKLNLDINKKISYEQSHRKCFFQRDYVRMEWMRQKLPKSQSEFDIVQQVIMQNKKNVKKTYIIMMHENIMMRQKKDGSLCWIDYENSILDFINDPQFGEGIKLTKCFDEIEIYGELDKMLPIFKKYTIQLEFTQQYHFLKKINSFDKTEVFRIRSKTDRNDYQCKIFYKKALNLTMEKQIEKELYILRRINDEYIQKYFETFENQEQIIIVEELIIGGNFDSYLQKWPLLSEEKASKFFFKLFKSLAYLHSKGIMHRDLKPENIGLRLNGNLENPCICFFGLADIVSLENESDEEDETPKYLFQRCGTPGFVAPEILKNQEYDCKVDIYSLGIMMYYSLTGKKPFDSEDYQQLLEKNEEGYVDISILKLSKEGLMLIEKILKPEPDERITAQAALNHIWFKTEKLSKLMEFKAHNNIKKIQLKSPQHRKSMQNSPKLLPIVLSKQSMDQTCGSPVQHQFSNPHRNSTNHHNHSIFRITTLNGSFSPKEVQSFSQSLYNNTQKVRPSGISPSFFAKTQRPSQSISILMPNIFPKKLEYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9U2</accession>
    <name>A0A8S1K9U2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Signal peptide peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0110358</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A22B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9U2"/>
    <dbReference type="OMA" id="PPLMISY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF23">
        <property type="entry name" value="MINOR HISTOCOMPATIBILITY ANTIGEN H13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="305"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8047298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="464" mass="54523" checksum="96907341BBF0FE90" modified="2022-10-12" version="1">MYFYILFIFIQSHCFELFIEKDKQYLIADDDKIDNRFKQESLEQTQCHPAKLYLENQNQKHTKFVFHNNQQKQITIKMHPLIFDTDVLNILQNSKREPYQIQSNQQEQFIITYECIAQNDQISWSLIILDFTVFQNQNNSQSYQIYFYKQCQPTEQYLHPLIMLLILAITLLIIGTNYGLQEMKLIESIKTEEFNTKTSVLFILSASVLLVCLYKFPSIGQIVLSIVIFFMAILSIQIILQDQLEKIFRKQKFQWCISYFISFLIVLSYFYTKHWIINNLIAFLITLLMFKIIEIDSFKTSTLLLSLAFFYDIFWVFISPVFFGTSVMAQVATSIDLPMKFIIPPLMISYNSPLMKCSILGLGDILLPGIVIKYILKFENLVNKGYCMYITSIIGYCFGLLVCMFSLVIYQQAQPALLYLVPMILIPVFIISLIRNQFYSLWKGQIFKQEKSAGIYELQQSEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCG6</accession>
    <name>A0A8S1KCG6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0180050</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCG6"/>
    <dbReference type="OMA" id="AKLWFYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013586">
        <property type="entry name" value="PSMD3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057985">
        <property type="entry name" value="TPR_PSMD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08375">
        <property type="entry name" value="Rpn3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25573">
        <property type="entry name" value="TPR_PSMD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PCI" evidence="5">
        <location>
            <begin position="235"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="245"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8051345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="484" mass="55576" checksum="C1F7FB9AFCAAF4F8" modified="2022-10-12" version="1">MEQQAAATQEKTQIQTSAPSAQQPQAQPQKPRTVLSEIKLQILLLEKAVSSKDIKAIQKVSIFVKKFRNTVKSHHLAKLYNTFFPNVAIQQGNDFDANFNEDLGLSAQVVLKLTKIIEVNVFIQCLYLIWLFQQKQSEAYEQVNVLGKQLLQQVQQNNKRYLDTLLGVIYEYLSKSHEKLGKLDQIRDVLFEGYRNACQNRDENGQAILINLILRNFIHYNQYEQSYNFLKKTEFPEHAFGNQQARFLYYTGLIHAIRGEYQEAYKNLTQASHKAPDNTAFGFKVQAIKVIALVELLLGNVPNRDTFTSPEYQQALYPYYRIVSTVIKGNLGEFQQEVARSENILRRDKLFNLIQRLPQIVIKAGLRRINLSYSRISLNDIHEKLNLPTQCNAEQVVAKAIRDGTLAAIIDHENQIVITKETNDLYGTKAPQEAYGERINNCLGLYNQAVKALQYQNPEYDYGEKQADDELTTDELLSLAELDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEA2</accession>
    <name>A0A8S1KEA2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0200329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEA2"/>
    <dbReference type="OMA" id="DMEQQMS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="240" mass="27825" checksum="08577EA1FFEEF828" modified="2022-10-12" version="1">MKNTSASKQANKTNVLRKSAENLPPFAPSTEKDKETSKLAHLEINLNRVCLQQDALMPLLQMIEYIPAIQRTDSVVKGLMRKIEENESKILKSEKLLESKIKNPDGNQDKDTKKQQKQLQELQGKVKDMEQQMSNLQRWYEAKLKEENQALQRNLEHKIEKSKTDIQKSVGSEKRVNTEQSKTHSKQVSQVQQPQESQQQYQYNVLQAVEQMVTTSLNQVRNQIQQEIDELREQVIASKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEC6</accession>
    <name>A0A8S1KEC6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">cGMP-dependent protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PPRIM_AZ9-3.1.T0200350</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEC6"/>
    <dbReference type="OMA" id="NDCIFKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05572">
        <property type="entry name" value="STKc_cGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000251">
        <property type="entry name" value="cGMP-dependent protein kinase 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000154">
        <property type="entry name" value="cGMP-dependent protein kinase 1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000089">
        <property type="entry name" value="cGMP-dependent protein kinase 5-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035014">
        <property type="entry name" value="STKc_cGK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="85"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="203"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="324"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="457"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="19">
        <location>
            <begin position="716"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAD8053478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="770" mass="89436" checksum="F6828C449F85C666" modified="2022-10-12" version="1">MGVCISNIQPTSTEIAVDQIIPQQLNKPSQSMSSLAVEPRRGDRKRQAKIEVVEEQIHENVIKQQREKSPFDFQMILNAFGNHFMFAQLDNNDKAKLIEEMYYVTSKDQEFIFKQGDKATLFFIIERGQCQIIINEEKKRVLKSSEFFGELALMYHAPRSATVIAIGDCGFWVLERNKFRKAVEDIQQKAYEINREFLVQVKFFDFMTEEQRDSIANVLITLKFKQGESIVNEGDMANSFYIIQKGTVQVTKQGQFLRYMNQGDSFGEQALFGNCVRGATVKAYNSDVNLLSLSREDITTILGEKIQLIIYTNMQKWAFEKHPQLRDLTKMQIQKIVSNFKIKSYESNDCIFKMNQFVDKLIIILDGQLEFEGQLFNNGQLFGDKYLQMDEQKRKINHDIKTNRKTTLSELPFKQFFECIGGELDTVVKKNKDRAGSSSLLEKRQKSNYSLLTLEDFIGLKLLGEGTFGNAYLVKDIPQQNMHVMKCVPKVNVIANNTERHIKNEKQVLELLNHPNLVCFHRSFKDQNYIYLLTEYIKGKELFHVIREIGLLNSYETQFYIAQMINILEKLHSSHIIYRDVKPENFIVMENGYIKLIDMGTAKVLKSRASKTYTLVGTPHYMSPEIIKGKGYSFSTDLWSLGICFYELMCGEVPFAGDEEDDPFIIEEAILNGKLEYPQILKDFKAIKLIDQLTNRTPELRLGGSFDALKVHPWFDGFQWEELETFQIKPPYIPKLQQMIYEDIPFLDMIRRDTSPDMICTQQVDWDQDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJN6</accession>
    <name>A0A8S1KJN6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0220071</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJN6"/>
    <dbReference type="OMA" id="NSMARFY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05580">
        <property type="entry name" value="STKc_PKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="53"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="312"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8054521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="365" mass="43003" checksum="655C806E3F2CCE8C" modified="2022-10-12" version="1">MNQLQIEELRRKLQQLKLERMNEDSSEEEPTFQSVQGQTNDKQLFQNLSIDSFEVYTTLGTGTFGRVKQVRIKRDVSRQVYALKIMKKHDILKLKQVDHIKSEKNILNEIQHPFLVQLKGSFQDQKCIYMLFEFVSGGELFSRLRKDGRFSQDITLFYVSEILLAIQHLHRKDIVYRDLKPENLLIDKDGHIKIADFGFAKIIKNNHTQTLCGTPEYLAPELIQGAKTGYGKSIDWWAFGVLIFEMLAGHPPFYDIEPTNIYKKILNGVIEFPKFLHVRAKDIIRKLLNSDINKRLGVEDEGAALMNHKFFRGVPWQKVYEKKIQPPWIPYLRNETDSQWFDKYPEEREDIPLIHDEQQHMFDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKJ8</accession>
    <name>A0A8S1KKJ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0220269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKJ8"/>
    <dbReference type="OMA" id="YRSFKIW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="288" mass="34027" checksum="CC1AA3358E9AF530" modified="2022-10-12" version="1">MSIINQELLQKVIQLINSQPSIYQQLNSTELDKGIDLIDIYSVYGDVDQNIYEEPGEIQFNIEMEQEQPQEEVKESIPLQNTKKSQITLDQIKNYDKCDQVYRTIAETQGELEVFLDRNQDIYRSFKIWSDQLNYGELYSKDPKYIEKIKFTLILLSEWLVAVCYKQQVKKRILIQTQTQPRIQYKANIFLEYIYEPKKLSENRRTNEVRRQTVHEILLAATPDMAYVLSEGFIISKLTNQLIAPIQGGFCRIKDWLIAVRGVTNLNMKEFQELLKISREYFSEQRTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KMZ6</accession>
    <name>A0A8S1KMZ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0250021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KMZ6"/>
    <dbReference type="OMA" id="RYNLHEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="300" mass="35823" checksum="33497B859FF47DCA" modified="2022-10-12" version="1">MNTFHEYQLQVPAIKQLEKINEQQNIEGNLTLSRERFNQKQKKLQINTSRLIPIANEKLPRLSQSKSPIQLQKTIRATTEYQGSSKQQSIKFFKTNTNTENDANIYTDDERAKLKFKRDEKKPKTTNYKVGLYENTQNKRFAEFAEQIRKLKDQLDSLQKYLNNSRKLFLVVRNIVEKRKNLRKILLKNGKDELVELSDIEKLCSVKEFVNINGEQKLIDKLNISLLDDIIYANEVLENLKIQNEILDIKEMADQVYFNKNYDYNRKKLNQQVEQNFKNQLRRRFKVIFHIYCYVKVQFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KNY6</accession>
    <name>A0A8S1KNY6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190353</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KNY6"/>
    <dbReference type="OMA" id="QTSKAHF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="210" mass="24439" checksum="0D5CF5DA30E42B08" modified="2022-10-12" version="1">MKKIFPSNTQNGVSIVDPDQTTVQRLHQSQTSKAHFSVVPSEPSQSSYEIIEKNKIQYLEFYGQYSEQRFMENMAGLMPISEKLKLLENFLNMFEEMMSCLNTILNNLILINFTAQDFYQIYNTTPENVANTLYKDIKTLEGLHLELQFSSSLRYLETVLEALDFNPDQSKKLYQLDVKLWSSQCYAHLNQHITSRNMKARDKTCCCCKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRP3</accession>
    <name>A0A8S1KRP3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminopeptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0270054</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRP3"/>
    <dbReference type="OMA" id="LDFFHDH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09602">
        <property type="entry name" value="M1_APN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000004">
        <property type="entry name" value="Aminopeptidase N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000010">
        <property type="entry name" value="Putative aminopeptidase N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012778">
        <property type="entry name" value="Pept_M1_aminopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02412">
        <property type="entry name" value="pepN_strep_liv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF299">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="4">
        <location>
            <begin position="56"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="2">
        <location>
            <begin position="243"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="3">
        <location>
            <begin position="529"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8058119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="850" mass="99660" checksum="BB6B95BFDA6D907D" modified="2022-10-12" version="1">MLTKQIAFKRSESLSQVDYKIYIELLKGKLYRGVIFISVQIHKSDGLLLDWQGGKLDGITVNGNAVEWKLENGFITLVGQENLQGLTEIRINFENEYSTTGYGLHSFIDSDDQQYLYSQCEPYHFSKIFPCFDQPDLKGTLKLIAQAPKEWKIISNEKKLEGNMQNVELAQVQQLGYIQHKDDYQIQEFQTTKKLSTYLYAIVAGPYEEIKCEQLHNGIEMSLFCRVSLKQYLQGDSNDIFKLTKDGMQYYEQLFQFPYPFSKYDQIFCPEYATGAMENAGAVTINDSQIFKEEVPIDQIAQRGNVLLHELSHMWFGDLVTMKWWDDLWLNESFAEFISHLCQSKVHNIPIDHWVEFLKSKIWGYITDQRNTTHPIFCDVQNTDQADNIFDGITYAKGAALLKQLFTMMGEQKFSQAMGQYFNKYQYSNTTLADFFGVLKEQFEGDYLLQWQQDWIMKAGLNLVELVSVNQNIYTFKQIATLKDLHPTLRNQMFKVGLYDQDGKELEVKLIQLNPVEETIIEFEHQNAFVLLNFDDGSFVKTSYSQQQANFFLKNLSNLSTINRVLVLRSIWDQVRDGLTSFTTFINGVLKSLLNETDDAVLQMVLEWCTGGVSFYCPIKHRQTFENELADVIIELLRKCPKENKNKRLILLDYLALWGTSQQNLEFLLQHINSDEAEFGFIGLSNGWRIIRNIFANLNIPKEQKWQLFEKWAQRDGTDEVRLQKRFLESLFNFDKLWEYFINYNKEESIQMVKEAMAAFNHQIHLEQLFNYEQKYFEILPTIFSTKSSEYAKEFYHSLYPINDNINSYIEYCEKTLLKSQNDILTKFLKESIDADQRRLKTYKFFLDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KU19</accession>
    <name>A0A8S1KU19_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KU19"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="387" mass="46492" checksum="5D9F41DE34E6A808" modified="2022-10-12" version="1">MDQNDFEIQVEVNDPKIQSGINKYTIYSVRGMDKNGQFDVVRRFSDFRLIRQMLVHKWPGCYIPPLPPRKALGNMDQKFIDDRMHSLQEWMRTISQLKYFWYSEEFQSFIKVNGDIEKALAQVPKLTYEEIINKYQDTFTDLSGREINRDIVQKINDFHQFLRKVSPMMENFKQIAKNIQLSRDSYQKQLIHFLQVLLPEYEQQILLEFTESPDKLVIQSIKLDEYPAQLFNNYLNVLNQDEFIVLFQEIRKESKQLRAFLETMTQREKYEQQKLLFEQKQKELQLELQEILAGKASIMKIFSTTKKEDKIAKLQVEIDSTTKDIENMQFICDILTVLLGYIEIDKFKLEKQNNYYKIAKRLIQYEIQLTQVAEEFWERIQQNYNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUN2</accession>
    <name>A0A8S1KUN2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUN2"/>
    <dbReference type="OMA" id="DKFNMFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="298" mass="35910" checksum="79427E247EEC5258" modified="2022-10-12" version="1">MIKRERFRRNVTIKREIVEKKNKNCKSFVNLENKNLLDCFHEQTRSISCYGTEFGQLNKFQIRFKNILPNYKTKQSIQENEMNEKIRMQTQNIEDETILQFQRGIQDINKSKTIIQTLKILKTSKQDEFVKQIKQPYINIKRKRRNSCYCQLCGKMNEKQIRHQNDPYFDKFNMFQQQRRNSICSQNRKKYQDKLKSQIQQENIKISYISQNKSPINKYKNVSRTTKLILEQTQIKILQQGQRYSALPSQRIKTEPTQSSPRSFKLYPIQLNKLRLGQSTYKQNHLKESFKTLANIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUP4</accession>
    <name>A0A8S1KUP4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0250358</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUP4"/>
    <dbReference type="OMA" id="EFMESIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="396" mass="47506" checksum="9BFF4C97FFAF3EC5" modified="2022-10-12" version="1">MLGMQYHSYQYYSYIDIEKLKIILEVDQIGDVTLYEDEKTIKYKIDRSKHIRLSDLIELIEIGFIRFHLGQLLLLFQNLLIKVKLMEDHKLEHNYLRADRIWLNLKEQTQLSITYQKIQYSISFTGYQCQFYDNLEQKEVADSQRILQIISLIIKRLKTNNIFLNKHGKSNNQIIDKIYEPILQSCQKGQIGDTINQLKILLTTYSYNSNDQKVSFDDNLIDEYKYSKRKHQKNKIEMQLQQLIAKLDQIPLVTELFIFQKIKEIRVNLKNWQSLQFNDNDEKLTYHKMLQTYNNNKNQFLQTAIVIITEMLDNIVKNKIDIIFKESMQQSEKGQIINTIIEFKLLNYFENTNDIFLTIEPQFYMKLIIDLAYKYIEDYIDDYINSQILILVYDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVU7</accession>
    <name>A0A8S1KVU7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0250069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVU7"/>
    <dbReference type="OMA" id="DYHQHYN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="2">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8056816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="305" mass="34972" checksum="CDB2097B9DAB92BB" modified="2022-10-12" version="1">MIANNELVLGDQPKQIKCPFCSNSTVSTVTFKNGYISYITCFLVLLIFGLMPSLLLIPVIMIITRTLIHKCSACNEEIGGDGKVFHVLGVKDSMMSFKIGEFGVILTKRIVIGTLLLILTLGVLWYRILDMPTQQWMDYHQHYNPNITITDTWNDYLEKCTKEKIFQNLQFQQSCQQKYIDRTIVDWKGYAIRVEDNQQSLMKFLHHSVNILVKMDPAESEFHPDLLLTADSEIAAELSSEIEKLERGSEFSFTAIIKSIGDFQNIRHFHLLNLTKLEGYIEIPPHLHQQSRYGNSQQFLASSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KW77</accession>
    <name>A0A8S1KW77_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enkurin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0290133</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KW77"/>
    <dbReference type="OMA" id="NARYQRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027012">
        <property type="entry name" value="Enkurin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052102">
        <property type="entry name" value="Enkurin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21490:SF0">
        <property type="entry name" value="ENKURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21490">
        <property type="entry name" value="ENKURIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13864">
        <property type="entry name" value="Enkurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51665">
        <property type="entry name" value="ENKURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enkurin" evidence="8">
        <location>
            <begin position="170"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="12"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="232"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8059720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="267" mass="30870" checksum="EAE24A301B78C62F" modified="2022-10-12" version="1">MQQESIYNLIPKEYVPPPKEPMYRSAYPSGLTPTGSTFNNHTTSRPKVNNINGNFELVRGPHTHKGQSNSLGRPKGSYKPDSTMFRLKNTGTMGSNQLPEVQQYKYPPSVRPPVPKKDEKPIHGLKSNKNFIVTNAVENILSAPKQIVDEKPWTEKKDYGKVPDYLTKIQQSISSEYEIIRNMHISEAEEMDKQKYLMTQEEVEQLKKGLKKKWESVNKEYQSITHIRMIDTVGLKRKKEQCEKELAQLEKDIEKLNKNYVFVDTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L035</accession>
    <name>A0A8S1L035_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0310133</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L035"/>
    <dbReference type="OMA" id="HTRSKFF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000003">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000018">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="95"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="95"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8061089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="193" mass="23279" checksum="308C5BCBBB05B8F0" modified="2022-10-12" version="1">MSNSTPIEIYINLINDYQNASTDDDDEQFQIDVQPKRKYFETIEEKRQYIEEYTKKKKTELCKNFQLTGQCKFGDECSFAHGQLELQAKTHLHQKYKTKPCNRYFNQGFCPYGIRCQYLHDELKDQNRFEKFLQESYQQFNMKPTIARKYLNNTERLDIQRFQQVLKTFTKKGFNVGLHTRSKFFVQLSEKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1T2</accession>
    <name>A0A8S1L1T2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0320151</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1T2"/>
    <dbReference type="OMA" id="NDMLDGH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="126"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="262"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8061788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="277" mass="32812" checksum="C18314AC6F660220" modified="2022-10-12" version="1">MLGDEKETQLSKYREQVIHAETLNMMAKYQLIFLKMEISMRKVALIIKLKGNQQKFYAFRKLKKTTNYSVDHFKVLFTQIALKYQQVGLIIQKKQKRNLHHAFLKVRLLFPKNKNDKIRRSFLQVIASKDAEIKNLQIKEQEITENITNQKLKEQELQSKLKQKELILTQLENELRKNSANKTLDSKLRNLEVENQEMQDRIMGTEDSVSLFIREMNDMLDGHEISTNLGIDSDENQSYEQPPQVIEYQSQRLNQNSRQQKSKNFYSNQLTRSTKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L289</accession>
    <name>A0A8S1L289_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0300053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L289"/>
    <dbReference type="OMA" id="NTMHNLN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051266">
        <property type="entry name" value="CLCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579">
        <property type="entry name" value="CALCIUM-ACTIVATED CHLORIDE CHANNEL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579:SF43">
        <property type="entry name" value="ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="336" mass="39454" checksum="3AE536F2045C5AD3" modified="2022-10-12" version="1">MQGQKIEYVKQILHSILTNLREQDRLCLISFNNEGKLLTGLQKVTPETQEYFAFVIDDLQCIGTTQLWKGTEVAFDVINQRKNKNNWARILIFSDGQDEIALTKIRKQLEYNYDIFTIDSFGFSNSNANKRLSAITNLRFGKHYIITDEQQVFKCLEEAFANFPFNLWDDVTITISTNSQNIPFDKIMITEIHSEGWVELQNQHQYQITIPNLEIGESFLFPIELAFQKFNDNIIDKSKPVSLLKGKIEMQNSITGKRMIKNVDLDAIFLYENDSNTLDFNTYFNCKQSPLVIWVCDQQQTQFNRKSYFELICENLQLKWQLQQQRIKQLYDEEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2J7</accession>
    <name>A0A8S1L2J7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Parkin co-regulated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0290108</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0290114</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2J7"/>
    <dbReference type="OMA" id="DLDYCYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019399">
        <property type="entry name" value="Parkin_co-regulated_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207:SF2">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN PARK2 COREGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10274">
        <property type="entry name" value="ParcG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="29500" checksum="02344B6080937E5F" modified="2022-10-12" version="1">MQQQQVSNKRIKPIQGHSPQRSPGKFCSNLLNISNKFAGTGGMTSSLAPKKAQAPQKVPKSGIFQALPMIEQTLFKKYYDRGDLPIAVNFSGAVRKIVWKCEPECLDYHLYLPIFFEGLRETEDPYKFLAVNGCEELLQKGETKILSVLPQLIIPIKKALATKNHDIMCITLKKIQKLVKSGQMIGEALVPYYRQILPVMNMYKNKRLNIGDKIDYSQRKNENLSDLIQETLETLEKNGGEDAYINIKYMIPTYESCMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3W1</accession>
    <name>A0A8S1L3W1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310328</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L3W1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="149" mass="17744" checksum="6BDAAD8F60971773" modified="2022-10-12" version="1">MASVNYLTLRPEQAYKIYNGKNNDALLILFRYLSSFGQRTRKTTTSYQYQGDVRVASNSQYLYKLMHILIQDYLLESINLMNEQKEDFKAYRQMTQHNHFDAFADILKEYLVKVEKIFSDKVKQYDVEELSNLQIFIAQEITICWTIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5F2</accession>
    <name>A0A8S1L5F2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0330263</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5F2"/>
    <dbReference type="OMA" id="FYFKENS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="219"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="88"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="262"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8062659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="402" mass="47120" checksum="52ED62BA8160B72A" modified="2022-10-12" version="1">MIRTNSSVQIQKLQIPLQQISPFNGQIRYSNPYSDTPSTPSKSIIGQQIRGSSTFTPNKSQQTFYFKENSNTIESKIQSLVNENCYLNQELEKARSEIQNNAQQYEQLYIIKERVSKLEELIDTQQNEIEIWKLKYQKAAAGDSRLEIQQMEQQIYNVIDENERLNKIISNLEQQQQEKDQEIKTLMMSIQKQEMEMKKQKDKLIVLEKANKPAFTCNPFAIDNKKPSKSSTNDLRQSKCKINEQDSNANRKKSDHAYKQIQTKSQQDYTDLQNQIKQLQTDKQQLQITIEELQEKLLNKELQKSENKDSQKDSNQEQNILILKQEVSNQQILILEEFKKYKLCQSQNQMIKEQLLQWEQFFKSFNSQISTPNEQEDGIDAAIEEFQSSSANCLKLIDSFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6P2</accession>
    <name>A0A8S1L6P2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0350030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6P2"/>
    <dbReference type="OMA" id="NCYVQIW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="273"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="61045" checksum="D396AEC74E962063" modified="2022-10-12" version="1">MSFIAPRLNCQNHQKCSISLINLTTGLPEEKRLYCHRCNLQLQQNLHSIEGIFEFAVSVQQSQNEQIGILKQDKELFAFVKNQLNQIQAYYNQLELEHQQLYQLVMQNQAIKPNDSIEVCLQKKQQCDKKELIKMAEQVSYLIKQNSEELVLQENNSIQYFSKLEQLRGKINILQDQLKLVNIDQVQQDIKNTQIIYPKFDNLQQIKEQQIVEGDRFMKIAEAQLEQSKIYDIKFNKENNLFVVGSSKLKNNNCYVQIWKYENNQISKIQDLENCHSGDVKSICFSTCDDSFFTGSQDNTIIYWKLEPQEWKKCQVLDGQSSGIIGLQLNNKGNVLAAVSNQIHLWKREFYKWEKTQVLNQSKKQFLCVSFNNDDSYLAAGSSDCQIYVYQYQNSQWKLKNTILDQSQEVNFITFTQDLNLIAILKDNNMNSYIYQPNDSYDEKCVSILTSFQVGYSKKGKVLAVSSGRSKETTIYQIEQKEGIKKIQQIQVKSDLVYSSNDGKVIALFANQKLEFYSDKNIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LC72</accession>
    <name>A0A8S1LC72_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0350194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LC72"/>
    <dbReference type="OMA" id="CIYDERY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="354" mass="41505" checksum="75F0CAE2F27D0F7E" modified="2022-10-12" version="1">MNTVCQQKTATPCRFETTQSYLLEQSPKIQYRRVNSPGLPDCSFRKSHKSSVDKRNYMTLGQMDIQQLLKPTRSLEESPEQKLKCYYAKGEETLDHYQHSFNPQQYMSLKEDKEIQVEEKPFESPPKMEVFQKPQLRKQMKMIDKIESDKVSVEIFMSEQDENSLIFTGITKRILPSPEENKQILMQNLQQLFTSIDKSTNSNKFQNYIKMESLEQSVFHQHLNKSTQQDFLSISSAQSIKQQKCSSQTNFRRNSAIIPKQQSKWNNSTKADKQKNNCFSNKQSKSPFLKAQNQKVSISKPKLQQVNLNIQSPQKVPTQKKQTQQNRSTVQKVRSFHITPEKIDDCIYDERYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCH0</accession>
    <name>A0A8S1LCH0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0360022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCH0"/>
    <dbReference type="OMA" id="AKWNGVE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035748457" evidence="1">
        <location>
            <begin position="16"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="132" mass="14562" checksum="EF5DD3E780602FE2" modified="2022-10-12" version="1">MKYCLIILIVVSTFAAIPTKVTNCMADPKIVFTSATFSVQPAKGVDETITLFGNANQHAELTNVLLKAKWNGVEAFEDNYPEDEVYDKGDPVSYALTQNFPTYTPSGKISCQFWFQNAKGTNFACAEISFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHE8</accession>
    <name>A0A8S1LHE8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0400180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHE8"/>
    <dbReference type="OMA" id="CQYLEIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="600" mass="71910" checksum="2BC34912F3BC5927" modified="2022-10-12" version="1">MGNNQSQNKNHPKPKSKKKEQLKKLFVIKKDILFSKLSQKTSSLITCFLNVQEFNNLLQVSKTTYQIFSQTSGCFQIECQRLLNIKCELIQSKNWKLILQSVMSIPIRTLPYGEAIKSVRYQIKQFPMFIEQKINYSGFQKAILYYENQFNEQKQIIKSFDYIEALNFASNNTDILLRVRQGFEIHTQLPELLMLIYNLHDYVKIFCQYLEIRFSKCNLEQFIQIWQHYQGWISSVENQTFDLIFLFNKIIDESLPQYKLPKYTIRHFMVCEWMKKADKGQLIVKLREDFRLQMIESRNKNKKSQLLRQYIQYLIDISTNQNNIQQYGYYNFQYCQELNQLINLAIDLTPKLNIEYQQDEELLIYIFGQYVYDYYVFELSSAFRVDQFKCEIKQFHQESQQMSKILVKNFEDQHLQNNLFVNEVQNFKYYLQSQYNVQLKIKSLFKQITIMDDDINNSSENIIAESLGTSGISLTSTNCSSVFSSVKNNDQLYHYIKFYQKELFNKIQKFCCKQQDVYQYSLEQEPSNEIIYDVPELAEYDYISHQNLMDLFYNRIKRSNAIYQLERRQTILTSNYQSNQQQIQTQSLNQAEQMLKCLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJI6</accession>
    <name>A0A8S1LJI6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0410232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJI6"/>
    <dbReference type="OMA" id="MCISSKL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029147">
        <property type="entry name" value="CFAP77"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14825">
        <property type="entry name" value="CFAP77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="205" mass="24029" checksum="379D13BAF9E2E0FE" modified="2022-10-12" version="1">MKPWNKNRANSLLLKDSVGAAKQSIYTIPNDIYFGKAIVHDLEGAQQVTSTWYYHNHSEQNPPDRDFMKLNKMCISSKLHDQKQFYLFRKSNDAKVFRRRGCSQIEMNLPDENFRYGKPYLPQSPMKNVLSGSYMNEAEQLMDKKYDAISKYKQQQNKRPSTATKHTKASKLAYQSLTKSLNKTQQPQFKLKEFDKVEPKTKTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKX4</accession>
    <name>A0A8S1LKX4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0310199</name>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0400124</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LKX4"/>
    <dbReference type="OMA" id="SNKKYRA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11380">
        <property type="entry name" value="Ribosomal_S8e_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.168.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_eS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8061219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8067125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="207" mass="23962" checksum="ABE8C696C6B8CA33" modified="2022-10-12" version="1">MGISRDSRHKRRLTGGRMPIHKKKRAFEKGRQAAMTKLVSGEKRVRRLRVRGGNFKFRALRLSDGNFSWASEGVAKKAKIVEVVYHPSNNELVRTKTLTRGAIVQVDATPFKQWYLKKYNVDLGAKKTQKKDQPAQPEVKKSRSLQKKLEQRMKDRVIDNLVAEQFQNQRLLVRITSRPGQSGRADGYILEGKELEFYVKKVEQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LN87</accession>
    <name>A0A8S1LN87_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0440037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF9">
        <property type="entry name" value="GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="383" mass="45457" checksum="F957DEB5E65B7363" modified="2022-10-12" version="1">MKKQAKSKSPDQHIEQIMKNKQQKLEEDEYQTLELTRMTSLNKINTDGQNFYINILHGNQEMPINLSQYLHEQYALTHSFKNYPPEQYYGNCEYKLKIIKVDQCKMEHRTTQMQFRLDEGKGIAYYRVGVEDDGTPTGINLNEMLVSIQWLIKFANNNKAEIILQKMHQGRTNSQKIAEFMVRRRLQDSIKTDIRVVMLGQVNSGKSSLIGMITTGKSNIGRQQFGKLFRQVVGFDSKGKVVNKINLLGQEKEDEYVFENATKIITFIDLLERQHCNNNEAMQNIQSQFPNYFMLVINAIQQXIYNYYNKSQIIIIEIEYRIFIQDNNYYFYYFVIKFMNSSCSLIIWLKLQSYSRNKLLNKQYLINYRTTRIQKFYFINYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LRX1</accession>
    <name>A0A8S1LRX1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0420054</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LRX1"/>
    <dbReference type="OMA" id="RLLYQLW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010301">
        <property type="entry name" value="RRP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13026">
        <property type="entry name" value="NNP-1 PROTEIN NOVEL NUCLEAR PROTEIN 1 NOP52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13026:SF0">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05997">
        <property type="entry name" value="Nop52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="303"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="374"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="335"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8068233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="482" mass="57507" checksum="6D1B89EEF2FDFAC8" modified="2022-10-12" version="1">MTTVSVDFLQQITLLDPTNRKQTIIELKKVLTKPLLISDYKKIWKALYYAMWSSDKFQNQQILATEISQLTRMFYSNFTAFIQFSRAMFHVFMLEWEKIDYWRINKFMLLFREIIEEQIKICKHFNWKTVPQLIALYNETVLNLTEFQTVQGASLHFIQIFVQCMAKHMSDSTVTYVQVKDLLEGFLDILKHSPNKTLRDKTIQYVYEYLEERQANESYLPAFDSKKYGNYVFKLASSENVNPQNRKSLYRVAEIFNFKGEQEESPQIQVEEAPKKKKTKNNNQEIQQIHKPQEIQQFEENYVQQQQNEEEQFNQQDEQEEQVVQQVPQKVQKKSGKEKEKVIKEKELQQQQQNNTKIKQKEEKAILKLHKKQEEQINSQYQQDSSIQDEDTIEGDDYSEVRKNPNKLKTLKEIMNSVPVYLFMNPAEKRKYFKSINMKFQDALQKEKGTHCTHNKSVHFDLARNKVKTFKQTDNVLRISKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LS46</accession>
    <name>A0A8S1LS46_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0470029</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LS46"/>
    <dbReference type="OMA" id="HFHLLNY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016650">
        <property type="entry name" value="eIF3e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10317">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="183"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8071208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="386" mass="44613" checksum="3E9879D8C83843E0" modified="2022-10-12" version="1">MDHFLRFINPHVFLGCVQFLQLQTSKENEALLGTRLFERLIKIGVSQEKIDQIMQQDNLNGKLASVTELLKLRQRALIDYVQENLWTVEEAYQALIILFDQGQYEKATEIVDNLYPCVEATEKLNHLKNGFLWARLNCKLIGLFKNTNNATTCIEAIKDLLKQEDQIKYNFKQRANLLHAGVLVCFISDDTNAFLELFSGEQFLEVVHTIAPYLLYYYTVALLINQQFNGNTSLAIIGTNANKSVPHFHLLNYVEEIIVKFQFNNAIKMMNDISTEIDKDFIVKSKKAIIINACKLYFFSTYIKIFNGVQIKQFSTYLQLNEEETESWLVNAIRTLNINAKIDGDKIIVQKQDINAQNTQLLKQLRDLQPKSNMLISNLQRIINIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LVX7</accession>
    <name>A0A8S1LVX7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0440214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LVX7"/>
    <dbReference type="OMA" id="CCGFKIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="251"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8069743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="317" mass="36699" checksum="067B24F953BF5BD6" modified="2022-10-12" version="1">MITVPQGERLECYKIDGQYIGTLTLNNTKPNNCCGFKIRTNNTQDIQVMPHIGSFTGQYFKIIVKCQKFQSNAKLQILTVDLSEQNFNKTDVKSLASYFDSYSNNSFVQKMKIEIITIDQNQITKQSNTVNQSISLNKNIEESINNQQDFTYEQNALLKKSEQIKQQPIRNSYTNSDPQQSVSRVSASEQRFLTSTTFQQVKQTSSKQSSNSQNNFQFDYPPPKPQSYPYQPQQPKVHNYQEKDFEEDTQIQKLNLKIHQLQIEYNKQSEELQNIEKQKTLLLSQESDQNQGQIYVSVQHIFFSALICFLIGYVATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXP1</accession>
    <name>A0A8S1LXP1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0510140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXP1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032273">
        <property type="term" value="P:positive regulation of protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008907">
        <property type="entry name" value="TPP/p25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12932">
        <property type="entry name" value="P25 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12932:SF9">
        <property type="entry name" value="TUBULIN POLYMERIZATION-PROMOTING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05517">
        <property type="entry name" value="p25-alpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="705" mass="82850" checksum="2CA424BAA5858CB4" modified="2022-10-12" version="1">MISKMIMDRQKKTQNIDENELDSFNRYLQRDNQPLNLGTQLSYSKIKVNVGMPVITEQQNETLGNTHQSTVQQSKNASPQKKVIGIEDIRLNIRIIFKFYASFGTRNNTRLLKSNKFIKMLADAQIMPNILSNRDCDILYAQQTKNNESLTLEKFQNFIPKLAKIIYPQLNVQQAFQKLYNENLSQLSQKILQFTEFGQQIQFLLQPISNQIIEFVEPIQQQLSNLHKFVFEDQSTNISKVQWQFMQFLTQCEILPNYINQSQAQLIFDNIQIRQPILNLQEYGNYLFGLNNFIESLIIISKTVPNLDEFTQFRLLLDKIEQSEGFSQYLRKLNRTQSDKVRLFYNIEKSRVYNKQENLIQQSQMIQNTQNNLSNTNIQIHNSNKKVDESRVNVSMMKQSKYLQDIDQSYISSLKKLFEFFAQNGEPSNIQSLKSTKFNKLLQHSGILNNLITITDSDMIYSKLCGVQSIKTQQKNKQLTNGKMTFGQFQISLGIIAEKCGMDINELIQQNILPLEKQMSDDNKEQILQILVDLLQDEQIIQLYEMIQPIFDWYFQEYTKQQNSILYLSEFLKFCQDFELSNILISQTQLTSIFYSVASLNQEGQEYTDQIFLDKSQFVEAISIIAIQIYSTIPNNIQRIVYLLERIFQSQNASKIYIKQNKLQTKFQNVIEDIKYRFLEKDKIHQEDEQYNFDDLVNGTIQQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYH7</accession>
    <name>A0A8S1LYH7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0460046</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYH7"/>
    <dbReference type="OMA" id="CQNISID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="151"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="151"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="215"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="217"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8070603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="464" mass="56048" checksum="954C834655AB05C3" modified="2022-10-12" version="1">MLQEQNLSDSDSYPQKKRGRSTNDRQMTSQEKLESQKQYQLFQLFYESCQNISIDFLYLKEYFNVGLNDCPHDVEILKIQKKLNNRRFSSNPKKSWSFDEKKVLVWVIGKYCSLKQKNCRFLNSDDFNEIAQYLFRRNAENIKQKWTSMHKTSLIAQPFTQEEDQQLSILFEKYKNDENRWKKIAQEMSKVNQIYRTSKQLRERWINYLDPSLIKIKDPWTDREDLELIYQIQQKGKKWTEIAKQLKRNENQVKNRFNGLLKRDEVDDDLNKLIDKILWRISKYPIDELKNQYEKQHFHNNHHQSLLLAVGNIESITREESYELTPCMVNLKTNQIYFTPNYMLQEILLYQQQDMGDGFEKIKREIEKFEPSQFRVASLSIISEEYQQQPQLQHNHQHFIHSFSEMPNFESSNQPASIEIKTYINPFYSLKNLQQKYIRYRTDLQLPKVMNSIPWKSQPQLLFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYQ8</accession>
    <name>A0A8S1LYQ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0460080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYQ8"/>
    <dbReference type="OMA" id="QDFVPKP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="121" mass="14871" checksum="9B7E53CA344428B3" modified="2022-10-12" version="1">MNQRKYEKQSRKSSQNKNQDLEQNPKRRWSKQDTPRNYDNINKLQYSFDYSTINNKYLQQIRNNTKIKQLIAKQKLEQQNSHLFKIQQDFVPKPKWQNYQDSLQSIINQQKYFAYLDYQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0D1</accession>
    <name>A0A8S1M0D1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P-type Ca(2+) transporter</fullName>
            <ecNumber evidence="2">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0500155</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0D1"/>
    <dbReference type="OMA" id="VCCGQFD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000123">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000027">
        <property type="entry name" value="Calcium-transporting ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000014">
        <property type="entry name" value="Calcium-transporting ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000028">
        <property type="entry name" value="Calcium-transporting P-type ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="852"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="962"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="998"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="15">
        <location>
            <begin position="9"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8072999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1037" mass="114620" checksum="E0FDBC8FEF57B8FD" modified="2022-10-12" version="1">MAEIDLTQPFYSYPIEKVVSSVKTDLAKGLSNDEAKARLAKYGTNELEKEEKESIWEKIKEQFEDNLVRILLLAAVISFVISQFEDHGEVHAVPPWVEPCVIFTILILNAAVGIWQDLDAERAIEALKDLQSPHAIVLRDSNWGKIEAKDLVVGDIVEIKQGDRIPADLRMVELKTITLKTDQSILTGEVNPVNKTTDPVVKDKAAVQDKINFLFSGTLVSNGTAIGIVCCTGMKTEIGKIQKEVQEAAKEKQEDDDPLSKRLDEFGDKLAKVVTYICIGCWLMNISNFSDPAHGGTIMGALYYFKVAVALAVAAIPEGLPAVITTCLALGARRMAKQRAIVRKLPKVQTLGCTTIICSDKTGTLTTNEMCVKEIVLLTGREASTVQVFPVEGTSYHPEGKIEKLESTLVKGNGLTANLKRLAQSMALCNESKLFTDKGRVQRSGLPTEAALRVLVEKIGKYDKSFQSKPILDAPEQYNEAISSEFTKRATLEFTRDRKSMSVLANSKNEKGNVLFIKGAPDYLLEKSNQIMNVDGEVVQLKAEDKAQFLTIVKNLAEKGLRTLAICVQEECGQLSTYDGPKHPAHKLLIDTNTYKDIENKPIIIGVVALQDPPRPEVKRSIEKCREAGISVIMITGDIKETAQSIAMQIGILHNQSQFATHSFTGLEFSQMGEEKQKKVLTQVIGKPSGLVFSRTDPSHKRELVKLLTGQLNQIAAMTGDGVNDAPALKQASIGIAMGIAGTEVAKEASDMILADDNFATIVRAVEEGRAIYQNMKGFIRYMISSNIGEVVSIFTSSLLGIPDGFNSIQLLWVNLVTDGLPATALSFNPPDPDVMQKPPRKHDEPIITEFVFIRYCVIGTYVGLATVFVFVYFYLGYEWAGDGHPIVTFTQLRNWGECHYWEGFKVANFDKYDFSKDPCLYFSWGKQKASTLSLSVLVVIEMFNALNALSEDSSLLKVGVFANPYLIMAIFGSMTLHCMICYVPLFENIFNTVPLTLNDWILVLAVSAPVVLVDEILKVFSRIRNAKLLEQRKKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M713</accession>
    <name>A0A8S1M713_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0550130</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="SNQGVWP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000320">
        <property type="entry name" value="echinoderm microtubule-associated protein-like 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000898">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055439">
        <property type="entry name" value="Beta-prop_EML_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005108">
        <property type="entry name" value="HELP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF33">
        <property type="entry name" value="HELP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23409">
        <property type="entry name" value="Beta-prop_EML"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03451">
        <property type="entry name" value="HELP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="53"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="89"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="138"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="500"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="976"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1225"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2053"/>
            <end position="2094"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2175"/>
            <end position="2213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8075897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2213" mass="253075" checksum="FF495461DF194DF2" modified="2022-10-12" version="1">MGNTQGTPSDLDSFRKYAGLTIKHVMMLRERLEMLSEADLSITKDAFSLAMKTKEEETERIFRIFDLSSSGKIETLEFVCALSLMAYANLREKCDLMFGLYDFDRNRTMNKQELIILIKTVLTTVNAISSKGECSIQEAVGIAQVILDRYDTNGDASISAKEFYSFVSKDPDIVKMLLSYGLISIQDLRFNFGEPQNNELPVPEADSDLENEIFKSRVIKTVEDEAVALGIETLMNGGGIEQLAWHQQLAKDNMIMDPDDPEDVNGNPPNATLEIDHVYGFRCFDTRNNIKYSIENELIYHTGSLVIKDGEKQEFFMEHTDDVTCLDVWEHLVASGQMGSKPSIFVWDSRSMELRAVFRGVLTVGVSNLCISNDGRKLAAVGMDEDQCVVVYDIEKGSQTRMNGRKDESLLANGKGPIVEIFDIKFEKSDKQIVIACMGEVNFVTYDNNVLRIVKGMWDSKYCSYQAILSIGLLENAVVVGSFKGYLLLWRVGRTTMAIPDAHKGPITTIHTCNKKIMILTGGKDGQIGWWDHNLKKIRAFNLANQKDILLNNIKITALYENMDQTAILIGTRGGQILELINSELKVRVNAHFDGKLEALVCHPTKDLFYTSGEDQMLMLWDLNSKQLKEKKKFDYCIKTLDVNDRFLVGGCGNGTVLLMEPYKLTQLQDFKDRDSEITCIKIVDEYLIAFYSSPYFEFFVYSIKQKFKKLLSIPGRPGNCISIDYSITQRILVTFDNQQLWCIDLTQRQLTRYKEDDWKSFTSIYGWQIKGAWPQKTDGTEIVTADRDGSKSVIAIGDKYGQIKLYKYPAYKINCSFYRYLGHSGRISKLRFSSNDKCLISLGENEKSIIQWNFQVEQNVVERSSTTHSRNRSAFKSELNQFDDQMDGNDNQKTNKPFLDQIKMDEAEEEQLKLPMNYLNLRYIFGVSTSYKGNTLYQQLKYAGQQKIVYPIGCLCVILDTKIDQSAPYRKQNFYYGHTQLILSLSVHPKSQIIATGTVGDEINLWDVDTRQTQVQIRGYHKNGIKNLKFSIDGTLLLTCGSDQFNSICVYDWQANRIICNSKVDKSPLTDCCFLGNPKIIQFVTVGKYMRVWEIKGRNLSFDQFPGQLSAVTFAFNSELVCANDQGELMVIKNKQTNRILGHSGRVNVLIAVKQNLYSGGDDGKIILWHSIQNQLQQIQQFMETQTPPLLSMDIRNDNIVFLTSTCDIKELVITAQIHSIDIIKGHGEDVRGLAISKYKVYTCSSDRTIKVWDIRNRVCSLNSEPYQYQLRSIDVYNNLIVCGDSKGFLYLLDEKLSPLGSVSTNFNLKQKTIAYEISRVKFDPRGKRVCVGAQGGPSHIEIWTINYSDNIYKFDKERQIINCGFTGGILQLDWSEDSTHILASSTNYELKCISLNKQRDVQYNYDIKWQTWSCIYGPGLQYIYPNNPGEDITAVWLHKTKELKTETGNIKEYVFITGDQFGNLSLFKYPVTGRCCRTYKGHSGPINWIQFCMETEAKQYAISVGQKAIMIWQLEIDEPTVLDAETEKLLGINIQQQKKTGLEKYGIPQQQQQVKDEEYVDKISLKPWASNIKEPSSYYKDPINQNTPPLIDLELEHVFGYRGNDCKNNIRFLKSGNIVYHSASLGIVLDMSLNQQKIFNMHEDDIISMDLHQDGIRVATGEINTGVIYVWDSNTLQALCKFNQLSKGIKSLQFSPFNQLVALAMDEFTTIAIFEINTKSESGGTLSFKYQSGKQVINEIKWMNENTFYTIGPQHFKEWIFSSQIVISKIPIQDKLILNTFLIDDEEIYIADNRGKVLIYQKKVLQHTLEHHTQQVDALFKTKDYLFTGGKDQLITIYDAKQIKEEFIIQFKLLDLSKQAFQPIIKSVCYFEDKLVVGTVGCEIWELSTKDTKINNQTKFTPKIMMKGHYAPQIQSELWGLAIEGDFIYSCGDDATLRQWSVSQRKQTLFIRTVQDSNFVDIKPDENGVYPDSVRGRSIAVNFDFIVVGFKDGGVRVYDRELKQKSYFKVAKDWISDIKISPNNEYIAFGSHDNCIYIYRIPEFVIHLKPLRKHSAFIKNVDFSVDSTKLISTCGSFDLIFWDITTGKQMPQGRNILRDEKWATWTNILGWPVQGIWRDDYNGQEINCVCRSNSPFAPKQPDNYYLLAVGNDFNEVLLYRYPCINKKSEPIQRRGHSSPISQVKFTQDDQYLVTMGGEDMTILLWRVIKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M723</accession>
    <name>A0A8S1M723_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0560113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M723"/>
    <dbReference type="OMA" id="RTTPKFH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019">
        <property type="entry name" value="KDA ANTIGEN P200, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="545"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="722" mass="87871" checksum="EFBCB92E43675998" modified="2022-10-12" version="1">MKVSLSDFGFHQPNAPEINTPRSLEACRREGIQPSEIVKIPFEEFQKRYKMKNLDHKGIEQFYKHYEERREKKFEELIKQRHIVIEDEKTGAWSCEGLSKSKVISKYGQQETSSLVEKEKQQLEKIKKKQQKEIEGMLEYELKMQEIRQENERKQAEEIAKQQQRDVELQKKRAEQEEQKKQKELEKQQKREQEEEIQRQKLKEMELKEKERLKLEELKQKEREEEARKKEEERKAIQEKLKKQNEENQRIQQELLEKRKQDMELKAEQRRKALEEQKLKKKQEAELARLQNEERIQQAKQRNENELQKLKDDFERKIKLSEIKQKQYEEEKQKKFEQQRKEAEKHSELFQKVIEQNNQMEQEKKNQYLKKIQEAEERRKQIEEELEKEKEQKKQLEKEKEQQRKQVLLLNEENNKQKIEELVKKINEKEDILNKIQEERNEQLQDRKTLDILKRFDKRENVERIMRKQEYDKQKLQEKIVEKMQRADKLSEDLDQLLSQRHNLRREIEKQKREIMNKFEKVREGKLPPAEIQKQLGITEPDAKFNNQSNPYRTFSSTRPKTNQVKERPDKLQSVRPAKIQSAKPEPIQTKDKIDLKPTSPIIEQQANRTTPKFHKKIEAKKKSEPTRPEAPSPQILIYLNKMVQKHNQDMMRLLMEEHANENLRDEKLNQVKGEEKAKLEKEYALERVKAQLKIEKLKQTQESQQYQYKLTNNIFDNDIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9B0</accession>
    <name>A0A8S1M9B0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="WKINNEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF15">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1247"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1288"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1337"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1374"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1448"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1555"/>
            <end position="1576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2548" mass="297828" checksum="4FB4013AB009710B" modified="2022-10-12" version="1">MDEAQKNYLNLLKKTFEKRQEFIRYDVVLFQQFRNKLTVHSAAISRNSEMLVCGCSKSIIILYDLISKQAIKATNKKQLQFRKHQIVTVAFSNCYDYIIAGNTMGDLLIYEISIDIQNQKFDLDLMRFVPQYHEKALQIIFHSSIDHLLTISSDKLRVTMISEFIQNENIDQSQHQICQNLGGATTSKLSYYNNHYYLYIGMRNNKIVQIRSSQLYSKPKNETFVYQINSFSSVISKLETLLAIGYQSGQQEQGNLQIVYLKQGKQIFSQNGITKLKISQLLFFNSNSHLCISSWDGTFHILDIKSKQILQTYSHVMHEQGVITFFPLTEVSEFPNLISIGQDHQIKKYYFGKIKEQLFEYIPFIFWVEQRIRQELQFQSELHNYQKLLYIESMGVDSVKMRELNQSIFGFEKFDPLIKRIIDYTEYKERAFNLYKRYELEMQQTMASQIQRPHKLPPLDIQQKNPQIDVPENLIQSGILVNPIQFQEPLKLFSEYFSFTFKRISDTIVFERIKLMVLQKCNFIFDEKYPFKQHLFSNLKRMKNLKYLDLSYNSLYLEDVLQILDQLKNVTLLDFLSLAHNNLGDSSTVKFNNSDAIMRLLDEVEAISLRSNNLSDIDGEAILNQIKHFKKLKILDVSGNLSFKYKTLQALQRITILSNKIEFSSQRNDFLNFLNNIKLLQENEQQQELEDQVKSQDKQSNLGENEKLIENLPDQQPSQTLDRSASISSSGNYMSLQLLCIQFQKEVCEQAMKTATDFLVQRKITNLYIYVVDNKEDDVWETSISIYYTRGLKKLNDYIFGCIGGAILVEFIKISDKKVFIVLEEYFVQIIFFGNIQLVDFVLYQLHLLESLLLNXIFGFEKFDPLIKRIIDYTEYKERAFNLYKRYELEMQQTMASQIQRPHKLPPLDIQQKNPQIDVPENLIQSGILVNPIQFQEPLKLFSEYFSFTFKRISDTIVFERIKLMVLQKCNFIFDEKYPFKQHLFSNLKRMKNLKYLDLSYNSLYLEDVLQILDQLKNVTLLDFLSLAHNNLGDSSTVKFNNSDAIMRLLDEVEAISLRSNNLSDIDGEAILNQIKHFKKLKILDVSGNLSFKYKTLQALQRITILSNKIEFSSQRNDFLNFLNNIKLLQENEQQQELEDQVKSQDKQSNLGENEKLIENLPDQQPSQTLDRSASISSSGNYMSLQLLCIQFQKEVCEQAMKTATDFLVQRKITNLYIYVVDNKEDDVWETSISIYYTRGLKKLNDYIFGCIGGAILVVLSCFVGIYQNIRQEGFYCIRRIFCTNYFLWKYLACGFCTVLTTSFGKFIVKLFGVEQSYFEYNPKIINLEQELRNIRYIGFILFAIYFVSYYFVVVFLPIIFVNTCQGQQWLGHLIYIAFACYSFLVEGYLGLTISNLKVTTNEYTDGSILARYFYIIKDLLISQLTKFELYTCICFIVLVNSCQISVAIAWIGVGIICFNLLLDLALIVRYLLVPCINQKKPSYIDIFTQFSTLYQMNIVTNLLEKWSEQNTIYIFFLKSDVPQKVVVSIIKTFIQDTPLLILQAVYLGMNPTQIKIQTFVVSFTTVIIHFIFSMIKMLSITRAAKINEEIVLTEVSKAKFPNKQIKIFKELKKFIRNLKKKFQTLIYEHNQRSENLITSLVDAEKALLNRPHTMQNFELYQKQKSSKILPARKGITQIGNKIQRINVSKNSKVNQEELQKLISVKASLDIFGNKVKSWKINNEIPLDVTDVLLNDVPRQFFTIDLDWEATQPWVGNMRISSLKQEIEGFKLAYLHFQNLENLNILDLGFGSGYVSLFLLALALKRKKVFQIQLHLVDHHPDAIKFFEQKLNTEFSHLNKMCKIQYYLHDCFDGLNLFQDKKFNFIHLGYATTKWRINKDIIPLLSEDGLIFGYEIVSSLEQKAFSWSQTQKQMVYSQNLEQYGIMEPLTIQLKGIDQSILENQIKQKLDILKYIKLYDQCGLCEKQLTKEDNNQNNRAKKLIQPKLIIDLTQSTTQAVIANYHRQSKLLFCQKTQLTHLVIKWEQLESIQMFVHLVILKEXKELKKFIRNLKKKFQTLIYEHNQRSENLITSLVDAEKALLNRPHTMQNFELYQKQKSSKILPARKGITQIGNKIQRINVSKNSKVNQEELQKLISVKASLDIFGNKVKSWKINNEIPLDVTDVLLNDVPRQFFTIDLDWEATQPWVGNMRISSLKQEIEGFKLAYLHFQNLENLNILDLGFGSGYVSLFLLALALKRKKVFQIQLHLVDHHPDAIKFFEQKLNTEFSHLNKMCKIQYYLHDCFDGLNLFQDKKFNFIHLGYATTKWRINKDIIPLLSEDGLIFGYEIVSSLEQKAFSWSQTQKQMVYSQNLEQYGIMEPLTIQLKGIDQSILENQIKQKLDILKYIKLYDQCGLCEKQLTKEDKQFINHYWGEGLCMSCANEQLEQQGEEIDIAKINYRSNIIYYTGSNCKLSQIKQTSILLENLTYPFGHKVGTIREYSNVCSSCNSKRGFGFLYKCLICDSVQYCNTCGLQLMRDNQMKYHSACSQDHPLLRIVFPQKTQKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MFM1</accession>
    <name>A0A8S1MFM1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0610191</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MFM1"/>
    <dbReference type="OMA" id="FNHQRSC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="392" mass="47723" checksum="C8310594FC3EEF83" modified="2022-10-12" version="1">MSNGQTKYKACKFCQKDFLQNEIFNHQRSCTSKLKDSSKKMTKETEINIQEKIYEPQVIPFQESQQNQNDKHNQIANNQKFNYSIPERFKGKVTILDVKPLPKDEYQKDISDQPLQYSNQQLQQEKFQQDVNLQIKYEQPKNYTNQQQYFDQYPQQQQQQQQQQQQQQQQQQQTNNWLYQEYPKQYANLQAYQEQFIQQKNYQICQEQPKQNSNQQSIQEKPQNLKINNQYNQLNNQTKCDHCNLYFNMQDMSQHQMNCSSYLKYLDIIQEYYQQGIQCKDENKSNRESVQMKSQQIISEYLKIYQTYKNIITQGRKLLDQGYFKRLKFRKTFDQKYCYFCKDHFREEQIIYNLECCLIKCHTNCLHKQIIKSTKCYNCQKSLFSSKFKLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MI40</accession>
    <name>A0A8S1MI40_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0620206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MI40"/>
    <dbReference type="OMA" id="MHRKLNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="177" mass="21022" checksum="BCBD1038A755573A" modified="2022-10-12" version="1">MHRKLNTIPPLNFVTHESYNERHQINGIQSEATSPNYLLETPVLTNKHQFRKNSNHLLPMVQQSSPYIKLPMIEEKFLTPRQQQINLMPDLTFKDLFKNPIYYEKNGLSPKGSEEILKKLKMRHRRVDFSKMVDIVDETTNSIIKESLNDHKEHRRLSKKTTRRFHLSNPTQLTEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ML09</accession>
    <name>A0A8S1ML09_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0650048</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ML09"/>
    <dbReference type="OMA" id="KQGAEMT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000210">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TH1" evidence="10">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="199"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="457"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8081098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="523" mass="60861" checksum="6F9A7374745E565B" modified="2022-10-12" version="1">MDYEQVMNDKKLREQLEGGETIVLTMKVIKFTGQNKKLPRVIVITNKNVYNISPSEGKGVKGFFQSLVNKSRIKRKIPLSAITSITISKIVKEFILHIPTEYDQRYQVDDQLSLIIQTLCEVYVKHNLKKIRCHFIEEMNLSQYTTTNYDIKKNIRRQLPKQGAEMTVDDVKIAMQAARGNVQTIYQKSNTQEISIEDFTLIKMLGRGAFGKVMLCEKKDTKEIFAIKSLRKEDIISRDHIEYLKTERKILEQTQHPFLVSLEYAFITQECVYFVMKFMIGGELYTHLQKVNKFNEDYALFYSSQVLLALEYLHKQGIIYRDLKPENILMDEKGYVALTDYGLAKFLSKGQVTQSIVGTPEYLAPEVITQQGHAFTADWWCLGILIFEMLCGRTPFFSENRNQMFRNIVESELKFPSTINLSFDCKNLLTALLKKKPNERLGNKGDAEEIKKHPWFKKMDFQRLLQKEIQAPIIPDLQSATDLSNFNPQILDEKIEEDQNQTTNTQALKKFDQEFYGLNYKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLK0</accession>
    <name>A0A8S1MLK0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0610252</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLK0"/>
    <dbReference type="OMA" id="ENGACQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="167"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8079301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="228" mass="27383" checksum="2DEBFDDB205BE132" modified="2022-10-12" version="1">MNDLNIYNILNYENYDQLVQLFNENGACQFYSSIYLHSLDITLYKEEPIKYLNKKNQNQFGIIKEIVCLNLKNKNQLPLIKIQVLLTTQFVSQYVNTKIADWLESRELFSCQDTQWICWSDIQGKIILVKHDEIPSYANKKQMVYFMRASFNHYTKQFNPPYDQWQRQYCVCGNPDNHEKRYVQCDICDIWYHMECEGLTQQQCDRLDKNKRLTYSCNSCKIGKKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPQ9</accession>
    <name>A0A8S1MPQ9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0690078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPQ9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="93" mass="10750" checksum="F8936571F6A2631C" modified="2022-10-12" version="1">MPLQLNKINGSSISKKIINNIKFNQRQGRGMQKIYREKIYRFIISRDGEKGGLGQIVGQNERYVIEGQLRYIDKFNFSLGLRILQIVGFKEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTY0</accession>
    <name>A0A8S1MTY0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0640154</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTY0"/>
    <dbReference type="OMA" id="PITWVRK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF666">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="350"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="6">
        <location>
            <begin position="38"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8080766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="436" mass="49371" checksum="A32FCDD6043D3037" modified="2022-10-12" version="1">MSKVYESVNNQNQIQEAEKNFDQIYMHEKPYIGKAPVKGTYKDSAMTLIKGYVGSGILAMPFSFYVGGWLLSIFIFLISAYMLILCVHYLIEVANAENKEDQGLTEVAEVTYGEKGKQVTKVILIAYQLGKAVAYLIFFISFFAHIFSNADSQSGAGNWIYLIMAFCIVLPLSFIDNMARFAKLSLFANTLILIGLVYITFYNFYQILEGDGIKENVMNLAHFEQFPMIIGVTAYGFGVLPLAFAIRVTMSEPTQFKNLFNVVSYFNLGLFLAFTLLSVWGMGSRMESQQIILFCLDKNPSSYIIQICYAFGLICSYPLQVLPAFQQIERIEKLKSYINPELKYSSFRRLAIRSLINLILGLIGYSIPKFAYFVNILGAIGGASLNFIFPILIHKKYFEKDELKRKSKFNYYGILTFGICGGLCSFIYTIVKLTSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MW75</accession>
    <name>A0A8S1MW75_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0660109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MW75"/>
    <dbReference type="OMA" id="YSCYIIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="42179" checksum="7398FFB2EB9E82BA" modified="2022-10-12" version="1">MYFYENNPILIVQFSCIVMSLLSVVGVIIVYILGKGWRYFIANLFFTQIVSMLFFYVPQLLTPFIFYEQNNSDEEYQFCQFKSDLDNTQCCINGTFCKLQGYFINSSFLASTLISLYSCYIIKQTLNPNLKFGQTKKQIYWIHFTIGIFTFAICLPMLFWANFQHYGTTWKNELIYYICNLEVRSNDKFTQSLFWLQSSITFILIIVSIIFHCLVRQLKSKIRINFTNEFDSCSSLNLYVLPITLLTLWTINMIQKLIDYQTEWIRQWLYVFQLFWFIPQLLLALQGFNYASLFFYAFHQQLVPNLPKSLQSTYMFFARISIYNLIFGKIQESKIPKDSLLNDTDSAKENESSTNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZ59</accession>
    <name>A0A8S1MZ59_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0740202</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZ59"/>
    <dbReference type="OMA" id="YEHIPRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004753">
        <property type="term" value="F:saccharopine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12189">
        <property type="entry name" value="LKR_SDH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000087">
        <property type="entry name" value="alpha-aminoadipic semialdehyde synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007545">
        <property type="entry name" value="LOR/SDH_bifunc_enz_cons_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04455">
        <property type="entry name" value="Saccharop_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal" evidence="3">
        <location>
            <begin position="7"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8085570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="547" mass="62705" checksum="5516B77A799D0C80" modified="2022-10-12" version="1">MNKLYIGIRAEDKSYWERRTPIPPHDCKYIMEKHPQIQIVVQPSTKRIFTDEQYLEVGCLVQEDLTQCKAIVCIKEIPLDKYIEGMTYLHWSHTIKAQPHNMPALDIMLKKNIRHLDYERIYDEKGVNTTAFPYAGIAGVITFLNEYGKYLLKRDIATPFLQIGPTYQYFNKKDAYQALNVAGQAIKDRGLPKEAGLPIIIGVLGSSGLCGKGSMEALSNLHVTLVKPSELKELVNTPNDPKHRRTVYVCPFKTTDLVRHQEDYDKEFTSQDYYNHPNQYTPVFHTKYLPYLTIIINDIYWDHKFPRYITNSQMKDLVQSGKSRLQAVCDVTCDMEGSIQFLKKYTTPDNPVYFYEPISESIHDEFDAKSPKDIMYMSIDFLPSQLPYDASIDFGIALRDIVPHLAYSDPTKSLEESGLPVFLQNATITLHGQLTHKFQYITHLRNINDQLKEAESFQPKKALKKVPSYLAIRLTGHLFDTGAINKILSVVQSGCKFNIVDIQAGQSDNQQTSCLLQLYSKNKGQLMDEVDKVIQLCESIDIQVDME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0P2</accession>
    <name>A0A8S1N0P2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0640091</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0P2"/>
    <dbReference type="OMA" id="KRMAINI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000140">
        <property type="entry name" value="Protein phosphatase 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8080664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="282" mass="31672" checksum="D1B13CD45A0E91B9" modified="2022-10-12" version="1">MGQFLSQPITQKMHDSQSNAILSCHTTSMQGWRLQMEDAHIMKVEFREDISIFAIFDGHGGAGISNYLADNFVDTLVQQPSFIGEDYNQALHDCFIQLDEMIKTNVAKNTFIGSTAVVALVVQKTLYVANLGDSRCLLMRDDETIELTKDHLPCNELARIRFAGGFVNEEGRLNGTLSVSRAFGDFEFKQEPLPANQQMVIAEPEIRKIKLNKDDKFLFLGCDGLFETMNSYKVMQFIEERLEKGLEPPLVLENLLDSSLAIDTTTGYGCDNMTAMLILLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1J2</accession>
    <name>A0A8S1N1J2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0780038</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1J2"/>
    <dbReference type="OMA" id="DKNCAWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF23">
        <property type="entry name" value="MINOR HISTOCOMPATIBILITY ANTIGEN H13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8087117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="506" mass="58287" checksum="F5C504D4BA38A047" modified="2022-10-12" version="1">MQILLLYLPLLILGQNVKIFIKEDYDEYLVYEDNEVQFEFLRKNLDKGVTCKPPKSFGVSSTSDVIGYSNQTLRIYNNDKVSYNVYALQTSEKSTTITLSTEVLSDDTILRPQSEILFKLIQKCPQKVDLTSENYWTPIYVNIGFNEVSKENTELIEFLMIFTCDKSFRDLTFDWSLPILLIISTILIAFLAKYTRILSFRWKRNGQDFQGFEITFLVIGIYILLYASGATIVIATGFSNFIRYFFIVLACTIGTFAIFFVSAEMACLLKANSFVRKYNLIIASIISLLIGLPYYFFKPWYLSDIISLAFIFLIVKFFRLKNLKTAAALMIANVILDSTFALIIHYTQPESYNTTVLQYLNCPLELQLPLIRMQYDKNCAWISLFSQVIPGLFLSLAYRIDKSKRTFTYGLSGFLSLIISEGFWVLATVSVKHSIPQSLFTHPFLLGTLTINSLSRAELHSFYFGDFLIDQSYYRLRGESLQDCRKPLFLLNMDSIPEFKVQPTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N241</accession>
    <name>A0A8S1N241_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0750061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HAAIPYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035818427" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1390"/>
            <end position="1412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8085772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1427" mass="163789" checksum="2266B71097F0A3E6" modified="2022-10-12" version="1">MNLIYLLLFVALLKTPCVQKCSQKIDSLTVYATKDEQLNWNLIDELFNGVGLTFSLSDTQQNQFKIVDPVENTGGSALYSQAAEKIISSKALKSNKYWVNQFVFLNKGTTSITLGIAIGEAGQNVIAPKFDKFITLNQIEDNLDCYDIEYIFDKTFIVDCSKIVGNTIKNVLYYVNDGDSYEYLIEEALVQAKHGRLLGQYKVKGQGGKEYRYLVRATPSYSVSQDGSISGDSLLEVFLLNNKGQPSNTGYILDGLTMSLLLNKQYDFEFNIVDMKVLENGDIYLLDAQYGIYVVNYIPNGAFQLKKFIDTGMGFAYAFDRNIFLRRDGTYSENIAVLGYGFVELIVNGELKSEYKLPNGIHYTFPANIDLSQEFIVVRHDDATYLFDIDAKSKDLLQLQVIPQTNYVMINPFLPDMIVLTLDKAYRYTLSQGYLQYNGSDDVQEKKMIEIIATSIDQICKIQLTYQIIESDDKQLYKYSDTLTPFPNQITEDTEPFQLNVLTSGPNEQYKQIVEFKDDATAVKVSLKTYWELKFPQELQKDLLFHDVLVGIQPSYFFLAQQNKDGKLTILKATHNSIFNGDATYEKIFDNISIGIKLTKSTFQMWECEYPKFHFAYVENKSVNLKVYSDNQVKDIKTIPFENGISILFLKNYLFLLQNENVHAYTEHGEFVKSIDLDLLVEQGYKGRWTPKKIFGNRKLRENLIFVVHQDHITILDFHTSYTFIKQIDIDESDNIEIAIGAETFFIITNEIIEYNFDRLNNIYKTKVVQLYDFELVQPILGDFCSDTGYLFINAKTKTQLINEDNNKYHILVIQPNTLQHEALFQALPIEDPQLISTQGTSSQMYVYLQTKTSTYVYSMLTNPILIIEPSINDPQFSASVNVSLMIYNFEDTPSISIKQPITVINTQTKIYIDNTKFKANSIEIDSKDYVKKEKKLGNDWYSGQISEFQIICEQCNKGKNGISITQTVEQSNFSIFKPYKIRSINNNLAQAIDALIFFNDDDTLQEIVPLDVISKNYQCFQSTQLEYYILSLCHDGNQYTIYATSGISQTGWFPLGEVLKLNKHTKKIELLQNDYLIILDNESFIISKINMEDQENWILDEEYIINLNHIQTKYEDLLTFTPNDFLITPVNKVDGIQYYKVLITSGEGIFFLDYYFKNQQQLMTINLEYVNLTKELQKIQQYAISSTQYLLIKELKSFELNTLNVFIQTSNVVHYGLKIKLAIKEGQSYLIYQSLESQFLLNRYGNWNTVGNVAVSKSHAAIPYQNQDKIVIAIYPINESNKPTTAIGSFSFTEDASTFNPLNFGLFFRNEKLYSNSPSSDTQQYQINSEPQLTFIQTTDNPLKSEKIVIKVRNDFSYSQIDFELNIIVNPDPPNPDPPNPQPEESSKLWWIILIIVGGLLLLGGIGFVTYRMKNKAVEVDYNIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N692</accession>
    <name>A0A8S1N692_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N692"/>
    <dbReference type="OMA" id="SIMQAKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000220">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000249">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="75"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="225"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="529" mass="62186" checksum="53EFBAFE63A7C776" modified="2022-10-12" version="1">MRSNNRFASFIGNLREIKESNLLYKVTNTFQKTINEDADMIRQRCLQILTIEPEKRSIMQAKYVQKYFERDFAYFSGQLPDDLYIRIFKDLQLERSNAAEVVFNIGDIGRKMYFIIDGEVAILIPMQEQQHHDNHHHQRKSLIIKKFDDLLKHKYAQYKLIAIKSKNDFFGEIAIEQRIPRTASVVAKTECVFATLSYDSYQKVLGQYQEKILQEKLQFIKCTPPFKNWSQSGQLILLHSCQEMSFEAGSFIYKRGQKGDTVYIIKEGEILIEKWDKQQSVTQEGDIYLKKNIIVIGLYSTGQIFGDYEVYQTNMKCRYLTRVTQAKARVKTVVLALSISQYIDNMRLNERDAWIRDYFEQKFSKKWLNKPEIQTQYISKSPIPSNNKQYIKSREDGRQVYKVSLSNNNFEDLELEQDNKKDSKDLQLLSPTQLHSQNKSNKTMRKFLSLQGNLFKTQQNYETSSMDIIIKQLKNKVIKSRENLTNESIFDISQSKFYSVKKPSAPKLKIGLSFKSFREGERNKSKFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6U8</accession>
    <name>A0A8S1N6U8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0820174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6U8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="12585" checksum="48AB4C0D2D19A0D3" modified="2022-10-12" version="1">MPLYSDSQSSLPRGFVKLGWSQYDNQSPSEHHLHQQRLERKFIGKLNHGSCYKQCSIWYVYIINNEDLTSQSQQYSSSIYITQIIIGSILICFDFIKQNCILFTHYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N8Q0</accession>
    <name>A0A8S1N8Q0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0810035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N8Q0"/>
    <dbReference type="OMA" id="RIPKQIP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="171" mass="20414" checksum="D74B77D3B710C260" modified="2022-10-12" version="1">MHNQLIERNAIETLLNVCYDEQPQYDDDYEQEIRQLLKRTIPVRPKKEDNEIQSRQNTISFRTPLRYQNEFRLKTEARSQIKQPKTQRIPKKIPQITLKQSIKPQAKSYRDINHKNQVPLKASIVSLQNSTGFSFLNASEYQNPVLVFQKKSLYLPRFVLRLKQQQLFNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9H7</accession>
    <name>A0A8S1N9H7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0770155</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9H7"/>
    <dbReference type="OMA" id="QKCVERD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000854">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="11">
        <location>
            <begin position="283"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8086906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="386" mass="44443" checksum="980C594D04C49565" modified="2022-10-12" version="1">MGNCNINEKKEDLMENNVMSVQNFQFIDAIGRGGFGKVWKVRQKKNKQFYALKVMSKPKIISKKSVQSVMNEKALLCGLKHNFLINMQYSFQDREYLYLVMDLLSGGDLRYHIGRHRKFNEEQTKFFSACIIVALEYLHQQGILHRDLKPENLVFDSNGYLRLTDLGIARIWKPENSQDTSGTPGYMAPEVMCRHNHGVAVDYFALGVIVYECMLGRRPYLGRSRQEIREQMLAKQAAIKRQEVPPGWSLEAADFTNRLLQRKPQNRLGNNGPDEVKEHPWFKDFSWEKLISKEMIAPFIPNGNEDNYLPSDSRRDSDDTMNEDQQIMLRRNSIQNLFNGYDFDNNASVPSSQIAISSTSSSRVTKQPTTTNTPKSAKLVVQKLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDM6</accession>
    <name>A0A8S1NDM6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0800046</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ICEHMAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000001">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000153">
        <property type="entry name" value="Dynein axonemal heavy chain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000002">
        <property type="entry name" value="Dynein axonemal heavy chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000002">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000001">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000001">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000009">
        <property type="entry name" value="Dynein heavy chain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000063">
        <property type="entry name" value="dynein heavy chain 6, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000049">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF69">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="1885"/>
            <end position="2023"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="2523"/>
            <end position="2675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="762"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3167"/>
            <end position="3202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3350"/>
            <end position="3412"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3652"/>
            <end position="3683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8088021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="5521" mass="636767" checksum="A7521F990B9BB194" modified="2022-10-12" version="1">[encoded data, 5521 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGN8</accession>
    <name>A0A8S1NGN8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0900080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGN8"/>
    <dbReference type="OMA" id="IKYHPAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="271" mass="31887" checksum="8E64E26C354C7855" modified="2022-10-12" version="1">MDLDEIFGKIENKEKNQKGKQVQKVDKQQKSIQNREQHQLKMKLNHEINRLELVKLTAENIYQQQINKGSADQFQNEMIDLQIASMIGDPDIYGDKIKHKNFLIGKFEKPFQLKNTADNELQQPLKNGLNRQLKIFKRSLGNLRYTAVTKLNSLWKLYIEDLLCREQSADSKFQRLLKADFHGALIQIVYSKCKTYEGQEGIIVREKKKSFIIIKENDQLSVIEKENTIFLMPVGNKTYRLHGCHLIIKPSERIRKDIKYHPANVFCYQYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJ56</accession>
    <name>A0A8S1NJ56_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0850138</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJ56"/>
    <dbReference type="OMA" id="VEMEPAW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:001087">
        <property type="entry name" value="Heat shock transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="4">
        <location>
            <begin position="8"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8090181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="216" mass="26183" checksum="E5F6FBEEFE6229BA" modified="2022-10-12" version="1">MSSKRRVGIAHFLLRTFEMLDTPKLKHLIEWSPCGEMFYIKNSREFANKVLPQYFRHRNFQSFLRQLNMYNFVKKRLKNGWNQFQHKYFKREAKNLLIHVHRRSNGQQSDEEEQTDQQDSIKKQQEQLQQIKYLQNDIVLTSSFLFQQSVILQGNFQHLKNKLLYQYIEEQKQEKFLFNTFSLLNKSQTNSPKLTQTILTQNRKETEQEPQLFVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJ96</accession>
    <name>A0A8S1NJ96_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0770060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJ96"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="117" mass="13923" checksum="F6FE94C616BE395E" modified="2022-10-12" version="1">MIRRSLDSKNKQGYWNKEEEAELHLNTYNNMLGNGEKLVNILIEHLTIQEINIIKQESTITYLEINHIGQLIYFSFLLIIFIFQVAIRFYFLITIQHSNKNLDLIMKILNLDLIIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NLT7</accession>
    <name>A0A8S1NLT7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0790008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NLT7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="33" mass="3652" checksum="6A6938A673F570DE" modified="2022-10-12" version="1">MIATITPTQTKNDNIAGFWIICQLNGTCKTQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQ58</accession>
    <name>A0A8S1NQ58_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0950140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQ58"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="50" mass="6041" checksum="4CA815CD07665182" modified="2022-10-12" version="1">MKTQIIIHSNFNKMIQHLNLQQTVHLQFLFGQFNSDIHCFKKDYKLFTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NVH1</accession>
    <name>A0A8S1NVH1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0930139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NVH1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8093515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="84" mass="10612" checksum="5093A9CA51881A7F" modified="2022-10-12" version="1">MKNKQEFSNEYKVMKRQQIPKQDYKFGRRRYQHDFHHFCNICIPQGEEYFEKTNKKHFVIPEGEIKQYRALENYYDQPKYYNCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXC7</accession>
    <name>A0A8S1NXC7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1030074</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NXC7"/>
    <dbReference type="OMA" id="NHYFIER"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848:SF4">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8097142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="235" mass="27408" checksum="8DB420E9AC45F1B0" modified="2022-10-12" version="1">MLPIQTFWSKNKSNILLIVRLIKLNSFGTSECPNIYEFKQKFNYLKLNIQEQGEIIPNQTLQWFLDNKEGNVLIHSQFGINRAPALIASVVQKITQCNLNEILQTISFKKPEIKVTSKIQEEQELFQLDQYFQQISAHLKTQIRSQKDCAFKCIKCRTDVFLSAVLVHGIKPECNHYFIERPNFIEEYEQDGKISCQKCNQRVGDFKYIGSKCNCGEYICPAYMYNKSKVDKKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZH2</accession>
    <name>A0A8S1NZH2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Oxidoreductase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1000153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZH2"/>
    <dbReference type="OMA" id="MNQWSQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019180">
        <property type="entry name" value="Oxidoreductase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039251">
        <property type="entry name" value="OXLD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21193">
        <property type="entry name" value="OXIDOREDUCTASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21193:SF3">
        <property type="entry name" value="OXIDOREDUCTASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09791">
        <property type="entry name" value="Oxidored-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase-like" evidence="2">
        <location>
            <begin position="48"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8096201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="102" mass="12283" checksum="6D008CF2BD2D0565" modified="2022-10-12" version="1">MNQWSQIATQFKQNIKLFDRIDESELCKESSFQINYQMRNIKYKKQRPIEPDQPDPDECCGSGCQRCVLEVYYEKLEQYEKDLMEWLAEQDENEDDGKNEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P2E8</accession>
    <name>A0A8S1P2E8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0990160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P2E8"/>
    <dbReference type="OMA" id="CQRKHIL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001175">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000683">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="112"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="475"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="475"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="538" mass="62927" checksum="FBBDC026D3F3A364" modified="2022-10-12" version="1">MQNKIQINYDLATFTTICQRKHIHNDITYFLYIIDDTMIFTNELESQQPKYQLELNFETKIFWCIEKDRQLTQFGFEYKGSVKYFFAKDEELRKLKSHLKNKVMFKDVSDFYQPLKLLGKGGSSKVYLVVDKDNKQDFASKCVEKRYLKEDGGFQALFNEINLMAILDHESVVKLEEVYEGENTFYLILEHLKGNSLHDLISKGQITQLNWDQIKSILWQILTGVARMHQLDIMHRDLKPENIMFKEINQITGLRIVDFGLATSTQVSNYSFPKCGTPGYVAPEIANLKDLSFKYDKICDIFSVGCIFYKLITQKDLFPGNDYHEILKLNKKCIINLDNLSIYRTPQAAIELIQLMLQIDPRYRITAQQALEHPFFSGGFTDRKLKFQTQKRVQGQEKLWQTSTFRMEKSDKLQLPEIKQKSRQRDEDEVEDEKIKIKVPVMNSPRLAQHAKRKNLALADGSPTENPKKSAFKKFSTQEFDQQSPDTCSPDSARQHPTLIINNSPKLVQSQTLKRKLTYKNYHQHQAIYEVDDEQKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P4B0</accession>
    <name>A0A8S1P4B0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1050056</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P4B0"/>
    <dbReference type="OMA" id="IWFELNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1040:FF:000009">
        <property type="entry name" value="N-acetyltransferase subunit Nat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021183">
        <property type="entry name" value="NatA_aux_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF2">
        <property type="entry name" value="N(ALPHA)-ACETYLTRANSFERASE 15_16, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12569">
        <property type="entry name" value="NatA_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000422">
        <property type="entry name" value="N-terminal-AcTrfase-A_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8097828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="782" mass="93341" checksum="77D0CE8E5B8B4130" modified="2022-10-12" version="1">MKQTELNTKQNNEFKSLMRLLEAKDYKKGLKHSEKLLEQVPDNQEVVSLKAIFMYYNEEREAGLALAYQAVLKNLASDFCWHIYGLIQKAEKNYIQAVKCFIQAINKGEENLQLIRDTANLSIHIRDFEGNSWLRQKLLNSKPGMVVNWIAFIFSQHLVSNYASAFNAIKLTEDVIKKDTQNPIKKVEINEFKLYQIQLAIEAKDFQRAKQYLHDFKNDITDLVSFYELEYDFYIKIGDNVNAIQSVKQLLELQPQNWKYYQMLQKADPQVDLSIYDNTLVKGRLLAQKEKESFQNSFLQFIDPFFQKSLPSLFREIKHLYTDPNKIEIMKTSFETYLNKSPIEQLWAMMLLSQHFYQIKDYNKSLEFINQAIEHTTTLPELYLIKAKVLKKLQQFKEAYEQADRARQLDLADRYLNNQTIKYALQANMIVLSQDLLSLFLKDGSDPYELQMIWFELNIGRTLLRLNYLGPALQQFNLIFKQFQEMCDDQLDFYQYSIRRYTLRSLLQMIDAIDKRYDTKYFIQSAGLMIEGLERLKLKVQEQKKLEQKKLTPKEKKMLAKQLQKEQEEKTIREQFSIEGHIFNREIQRKFDLSGEQLLNQLKTSEDIIKMQNRFAQVLIHTNLNNKEVNFQAFKQIVCLYINQQRPLLCAKLLNKLRNNETEQNRIVNHKYQLLLIQFLNKYQGTLLPYIKEYQTDLIQFDTQFWNNIQIKTQLDQTIKLHCERIIQEKPFEENLESEDLQFLQEYPQAKTTHPFYKYDPNIEWQNQQIKEYQEKQQTQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6L6</accession>
    <name>A0A8S1P6L6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1070054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6L6"/>
    <dbReference type="OMA" id="YTMSDSA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="318" mass="37964" checksum="FBCE6FEBC111C7F4" modified="2022-10-12" version="1">MNNKQFNKKRIHEVDYAQKVLNTIKPDIQNIKLNQNLFHAQQVARHSSTLIKNIQKDVKSTLRKYDSQLKLSQSNRNYTMSDSARKYSSEIPRKTQLSTEKELESLKQQQKILMTLLNNLQQQEKLIVIQHQSSEEQQKQQCIEKQWYQQKEKQLQEIKQKQQQIGQAQKKKMQEADIIQLDISRLKRSTHNKSNSSQNFFSPINKEKINQDIPILKMNKSHSKFHQNNLESPKKWEQYGMINKKNNQKNQQRIETHQYINENYSQQNRKQNIELQIRSGNLNSRDSHQSKQLSDDLTYLINDLNEFNNKSKRFSIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P728</accession>
    <name>A0A8S1P728_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1070168</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P728"/>
    <dbReference type="OMA" id="LMILVYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000351">
        <property type="entry name" value="Copine-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF106">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="123"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8098693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="581" mass="66916" checksum="3570D13D102A2A45" modified="2022-10-12" version="1">MNDQQNISQIQQKLELFISCRGLANMDLFSKSDPFVVLQIQQNKNWFDIGKTEIVYDNLNPNFTKTFIIDYFFECQQPLKFIVNDDDGNGKFDFIGSVETTLGSIAGSRDQVLIVDLKKGTKKTGSLIVKAEQVRSLNQKLRMQIYGSQLPNTRFLPWQSTCPFFRLYRESQDSNQNLLIYESEQIKNCQNPQWRRLDITLQKLCNGNLEKLLKVEVLDYKFQGNHQQIGHTQFRVNEVIDMKQYQKQLLDRNNNEVGLITFNDFKIFDPPTFMDYIQSGTQINLIAAIDFTGSNGSPNTSNSLHYIDTQSGKLNQYQQALLAVGDILLNYSYDKKVPMYGFGCKPNLKNFKSTETLHCFPLNGNPQDPEVEGIDGIMQTYSYALKNVQFNGPTYFNPILQEVMKIAALSKDMAKDTYYVLFILTDGVIHDMNQTIDSIVASSHLPLSIIIVGVGDANFNDMNILDDDEGNLKDSKGNQSYRDLVQFVPFNQFKDNPELLAKNVLQELPDQIVEYMQLVDRQPKFQQKQKNSQDFQTPTPDNFNKFQISDEQRQQLDQSGRFSISNNEYYKQQNQEKQQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCQ6</accession>
    <name>A0A8S1PCQ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1130089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCQ6"/>
    <dbReference type="OMA" id="QITSWCH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="186" mass="21430" checksum="B0AB9D10D8B07F38" modified="2022-10-12" version="1">MNKQFKVALIGNSRSGKSSLIRSLKGEEFLYETQETIGIDFYQRSLDFKNINYTFKLYDTSGCEAYRLILNRLLSQIDAVIICFDASQERYQEQITGWCHLIENELRTIPTFIVGNKIDIENFSLNSDISSKYPVFYASAKTGENVQKCFNKIFMSLIPKKHSMIQKKPQNKQSTWGLLCSCCKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PDB4</accession>
    <name>A0A8S1PDB4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA damage-inducible protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1130090</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DDI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PDB4"/>
    <dbReference type="OMA" id="NTHTRHP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05479">
        <property type="entry name" value="RP_DDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14310">
        <property type="entry name" value="UBA_cnDdi1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01796">
        <property type="entry name" value="Ubl_Ddi1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033882">
        <property type="entry name" value="DDI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019103">
        <property type="entry name" value="Peptidase_aspartic_DDI1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15397:SF3">
        <property type="entry name" value="DNA DAMAGE INDUCIBLE 1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15397">
        <property type="entry name" value="SODIUM-GLUCOSE COTRANSPORTER REGULATORY PROTEIN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09668">
        <property type="entry name" value="Asp_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A2" evidence="8">
        <location>
            <begin position="202"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="6">
        <location>
            <begin position="379"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="326"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="346"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8100771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="424" mass="49067" checksum="8ACD1EA520B57A95" modified="2022-10-12" version="1">MSYRLQVTDPVKDKISILEVDQTLTVLDLKALIEVEFQIAVARQQLIYGGQFMNDNDTLSKYNMQNEDLVLIERKPKQQRTPLELEAIKFIKHIQQNPHLMEAIRIKDPKLAESIEKKKLAGVMEYIQKQQQKKFQQQQEYIRKMQQLEQDPLNPENQKLIEEMINKKNIEENREYAEEFIPESFGTVTMLYIELSINRHPVQAFVDSGAQSTIMSKACAERCGIMRLVDTRFSGIAQGVGTQKIIGRIHVVEMQILNQFLPCSLTILDGDGIDFLFGLDMLKRYQCNINLKDNCLIFPNEKLSVPFLPEGQIHKRISIQQEQEILKRQNSDSQIEEEQPIKSSNQPQQQQTQQQPQQQQQQQQQQQQQQQQQQPSHTRHPESSIQTIINIGASRAEAIIVLDQTNGNLELAASIIMQQKYGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PES8</accession>
    <name>A0A8S1PES8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ABC3 transporter permease C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1150129</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PES8"/>
    <dbReference type="OMA" id="FSFMQKS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32522">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32522:SF3">
        <property type="entry name" value="ABC3 TRANSPORTER PERMEASE PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="480"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="576"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="600"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="629"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="666"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="940"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="987"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1031"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="7">
        <location>
            <begin position="426"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="7">
        <location>
            <begin position="944"/>
            <end position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8101464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1070" mass="121010" checksum="644E9E7A0905ADC0" modified="2022-10-12" version="1">MQPQPIKRKDSIAPQGTSMSICSSLLFMIGQSCYEIKRRPCGYCVSFFSVLIVVAASAVSQSIIDRAPLIFLKSAEGPAGQSDIVLMANPNYLSEEHEKSPQYLMSIYSNSARQQGYLIVDFLNFTRVKEVLGPDRADQASPRIEQQVNFTYLQKTGECENSVNKAQQLLSKRQEFGSEAHFNDLRDGQQLGQRCGGVQPHPNAKFVAIDTQKEKKIGLGWEYPFDELKETEVIMSRKLAETYKLNVGDYMLIYGEFYDFYASYYIENYYEQIGKQFNLTNYNDTFINQEFSDLKKATQRFYLYQVKGLLDSTYGKFPDGDADKLIIVEHKHLFNNLAYWSWDKPFLKAMYTANPEDFVDEIRINIPDRFNIYMDSNYENIQGKITKYASNIRRDLGVYPCNMDLPVLQQLYDSRFGQLFLGIILNMIIVILFLLSVLLLYTLLLISVETKTYDLGVLRVLGFNKLGVVFMVLIQALSYVLPAIIVGIILSIPFLLYASSALKASIGVEIEATPTTNAIFMSLGLGLLIPLLSSYVPIKEALSKQLSFALDINRSKSTAVKIEVDLEGKSLPWGRIQFAIIASLFGICVYYFLPLALLSFNIGLLLSIFFFILCGMLLGLVIFAFNIQYLAERFTVYIFLFWASSAKKTMVLKNLAAHRIKNRRTALMYALSIAFVIFIFVGMSVQIENQATQTLQQHGCKLEITSSNGYLSPFEFENIILELGGNITSFAWVTDSLDSYMQRLGFSTAYMTHLGQVYQLRPKIVGVSHTLFDMGYNQFLKVEDQINSKLNPVEQLYTSRGSQSAIIGTSYADQLNIKIDIDSTFLLIVYNNTFSQYHQMRASSIITSAPALKFSSLPSVQEQNVVVSLPTFRRLCGNLIDAVENYPMKRLLISVSNSGNIDTVYGNFKKYIDSKGVSAKIWDYRDYETSIKQSQDLIQIIFSFLTGVVMVLSFFSLMTSMTANMLEQVKEISVLRAIGNTKMSITIVYIMEAFTLVFSSSFIGLMVGFIIGQSMALQQTLFTQLPLIFVFPWQMLIIIIIIAMIAAIISVVTPSLQILSKEIAQIMRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGJ4</accession>
    <name>A0A8S1PGJ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1170063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8101971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGJ4"/>
    <dbReference type="OMA" id="MEFRRQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="226" mass="26441" checksum="719BE38CBDDCFA64" modified="2022-10-12" version="1">MSQDISSSFIQFRRQISSKIKNSPLKNQDRYMTPLQKSDNPVGLLKQSITNNLTRLKLKSTICNIGLTPKRNSNISKLQNSYNKRQSETLKSPKTSLYYSPSNCQQSYLYQLQQPSTYRQQDYLKQQNEIRQYSCKYSKIMNDEQGHYYSQLQAKLDQLSDAIGTKIDRQSNLTEIDNLISLSQKINEKSKVEKFIDILQFESKVLQNDILNIRSKQQRFQDSNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHM9</accession>
    <name>A0A8S1PHM9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1170165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHM9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="124" mass="14601" checksum="A0E162472BFB5666" modified="2022-10-12" version="1">MEEQRKRLNSSMTYEEYLNTFEKAPKKPCLKPKQEPDVGMDDLDLLSDTQTNIPQVKNKIYCDPNTVIQEVEEDGKTPIVWVDQQLTPSPMKTVRQRTQSENIQFTFISFVEREIERKGSRKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJ34</accession>
    <name>A0A8S1PJ34_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1180125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJ34"/>
    <dbReference type="OMA" id="FWIGKTF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="400" mass="47672" checksum="2AE3DEBE3CF3CF7D" modified="2022-10-12" version="1">MQNLSIYTFSNIIKFLQVEEIYSKLTISKLLIQQLYHPHVQKVILSNYFPSKIVQQIDPQKYIQCIKSLKGCKIKEIPFWGIETNGGLSNNSLRFWIGKTFMKTNQPQVGIRKLENSFLSGTLAFNQISFKKALLEYAKNFIDFMGQEITYHFFPDWDEIEDNQIDMSKIRIFDIFRSFQIFSYYYDEQDWNCLKYLRDYAYVKHEQIDQMLEIDPYNLKDQDHQIIPQGISLVQEISINRRTQMTQIIKSILIFTSIQQYIPNYPDEIYNCQTPEKLYYYILQHPQFMPSQTNISLLKNMNPNIIYEDEHQLDYWYCTFIPIDDKQPLIWAQFVNPNFDEINLKFDKQTQRLAKSIQVRILDACYQENPYFNGIGIAYVSCKGLTLNEVNWTIIKELPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKS9</accession>
    <name>A0A8S1PKS9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1220081</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1220083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKS9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019399">
        <property type="entry name" value="Parkin_co-regulated_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207:SF1">
        <property type="entry name" value="PACRG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN PARK2 COREGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10274">
        <property type="entry name" value="ParcG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="250" mass="28695" checksum="6554D81FF8154899" modified="2022-10-12" version="1">MMYKQSPKINSNEQRLNQIHRNYVAADYNQNYQYSLVDPHMAQKIMIAYKHQKIEKKPLVAKKIKMVDPFSNKYQRDINSFAYVYEAGGIPCKINYNTQGNMKIQWIPDVNIQTLPYDPVLVTCFNGLLEDVHPYLAIATTAIQFMLSNEAAQEKIIQVLPKLVLPLRSALSSKSDKVFGSALKTLMALSNVVGSHLNKYLKLFLVPIWQRQNHQVFGEQIRIILTVLENNGGSEVFQVIKQKIPTYSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRV8</accession>
    <name>A0A8S1PRV8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1270044</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRV8"/>
    <dbReference type="OMA" id="YLLXHHA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000574">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="225"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="484"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="58"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="188"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8105383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="574" mass="66413" checksum="2F7FD067BB595FAF" modified="2022-10-12" version="1">MQQSTAKNLRDLLIKQQLNSKIHFKGEPIKAPVRKQSGTWECQCNLKHCTMCSRKKVNQSPMIRQSDSKKDIKVGSSISAHQSPNREKQSQILMNRQPSAKKPQYAENIIYHKSNVSNLQVYENSYQLKSKVIPKKVESKSMLSNTQMSFKLKLTNKYNSANSVNSINSQSRSEQILNSISESAPCSPKKDKQSHGRKSELNDKTQLVAQQIENLPPEIVGRQHFKFLYVIGKGGFGRVWKVEMKKNKKMYALKEMAKAKIIQKRSVNSVLNEKYLLEHLHHPFLVNMSYAFQDRENLYLIIDLLTGGDLRFHLGKMRRFSESQTKFFVACILLSLTYLHQNGIIHRDLKPENLVLEEDGYMRLTDLGIARINKGNNAGDTSGTPGYMAPEVMCRMDHSIVTDYYALGVITYELMLGRRPYNGRTRQDIREQILAKQVQVRREEMPNYWSESAMDFVNTLIQRKPERRLGANGIEEILHHPWLKGFPWDDLLKKKLTALYIPGAVDNNFDFQNQISEETQLNDEQQLENQVMLRQDTVQALFDGYQYDVDLSKLQHQQTQKNTQRKVQQTPDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PT11</accession>
    <name>A0A8S1PT11_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1310035</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PT11"/>
    <dbReference type="OMA" id="TKQIMHA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="110"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8106530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="344" mass="39956" checksum="0101748E3E9D3D4B" modified="2022-10-12" version="1">MFPCLEKVYQMVLQTQLQFLDNDPILQIKLQDSYRLILKKEYLHIYKQDEPFISFMICFPNLIKWYVVKNLLIGFSINGYEFTSHQGDLLKKLKNILAGRLFFSKVQDFFYPMKILGSGSSSKVIVVREREGGEEYASKCVVKEEMHFQEIEINNQLQHPAFVKVKEVYQGETSYYIVMDLLSGKTLQLFLGNHKPFPLEQTKQIMHALLEGVEFMHSKNIMHRDIKPENIILEMKGGQVRLKIVDLGLATYSTLKRFRYPKCGTPGFVAPEVINLQNSNQTYDKICDIFSCGVIFYKLLTGRDVFPGQGFTYVLELNKKCNIDFSYLILQQLPVNLTVCIINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZ20</accession>
    <name>A0A8S1PZ20_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1370071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZ20"/>
    <dbReference type="OMA" id="PFESNEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="147" mass="17406" checksum="E154BAD040EC5CFA" modified="2022-10-12" version="1">MQNNNLEQSFANYVNKLNSYSVLKINQDSQNKFGSSNLPKLIVPTPFESNEMLNHLERAKFPKKTDLNKTIWIELKQEGTKSYNKLTNELFQQLEPKSVKLNTTVQRLNFRKFQLKIPKSQQQLMRSRQKYLDQLLIGQQRIIKTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1H3</accession>
    <name>A0A8S1Q1H3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1390071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8109071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1H3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="129" mass="15230" checksum="988194E59AB19789" modified="2022-10-12" version="1">MQKVYADQFNARMLNNDIKHWIIGFEGAKGTLYQREKFELQFKFSNEYVEVLDSYLALFSLKIANRITSSNINNQNIQVIFIGKTPELEHIYSVNQYYLMNGQLPQMYHLYVSQSNPCYQVLQKNETSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q252</accession>
    <name>A0A8S1Q252_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1410094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q252"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="188" mass="22217" checksum="6EF0C75E8F789DA3" modified="2022-10-12" version="1">MFSEQILIFPSSILLSLNQNEIQEQLMSSLNILRPLHQMQFILLLKEQITNLANDVYVQEKGQTYVEKALNTLTSFIDFVKLEFEQIKYDGQSFKFMTQKLLHEYNSLIESFQGMTYTNECLNVLSVSMPYFWKQLLDHPLNHELEISELLIKKDGFKQIDSNLQILFDQYLQRRKITQDLINALLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2A0</accession>
    <name>A0A8S1Q2A0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1390024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8108979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2A0"/>
    <dbReference type="OMA" id="MMILDEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="565" mass="66457" checksum="FECD3506CC60DA2E" modified="2022-10-12" version="1">MGNQQFEANESETNITQYYQNMLNQNKENIPYQYQNTPKLLSAESRNKLRPTSKINLDMKLCSKSSEKQFHGIQQKMFQETPIVRNPLEKLHRKRSSTKIVQENDESHIQLLDLLKNMVRHQDSTMQSSQVKITEQSCQEYDKKKHMINQNQYYRPQIEDIPLQLNNRKRSKTFEESFRTLISNEDRCQPLKMNRRESKEEEALQFILQNCTQTQSRIDQQISNSITFESLESDWQALTKKNSKQLETPGTAQFHGLNKDDKIFELSTSNFIDQNNEDYQGSRRPSCLESTFLIPMVLHSQNSLEFDDSKDVYQNSKPTQPYEYKRTQIPMNGLFSKQQSQNHLNKLDSKLNSTNRDLTQPLKKIIQTQATGLPNKAPLKHKSFHQQSQNALSLPLQMINSKGNLGFRSNLSLIQNVPQSSLSSKNGRNQFDSKIMKTKQTITHPEEEWFDNSKLEYKKIMMILDEQQQQQQQQIQNFSIQNKSEMISNTFNSSDINCSQRFQLDDFKLKPHLEDKNTQTQLKDLQKNFINRLCQPKNKCSSRITENNPYWKLRELDQSKRTNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2A1</accession>
    <name>A0A8S1Q2A1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1410062</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2A1"/>
    <dbReference type="OMA" id="AYKGWGS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07830">
        <property type="entry name" value="STKc_MAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001171">
        <property type="entry name" value="CMGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000545">
        <property type="entry name" value="CMGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="315"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="315"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="329"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="408"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8109588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="432" mass="50351" checksum="A484DEC7B725EE3E" modified="2022-10-12" version="1">MKKFRELKKTGDGTFGVVIKAENMQSHDLVAIKKMKQKYHNFEECTNLREVKALMKLQNHPNIVKLKELFLDNDTLCLVFEFVDKSIYQMYIQQKEMGKTIPEDQIKSLIYQVANGLSYMHKHGYFHRDLKPENLLVSNNGVVKIIDLGCAREIRSRPPYTDYIATRWYRAPEILLKQANYNSPVDIFALGCIMAELFLNRPLFQGNSELEQFNKILSTLGTFTQLEWPEGSRLVSQMGLALAQFQPLQLQQLIPNASTEALNLLTQMIRWDPNKRITATQMLTHPFFYNIEKIAPPIIFEEQIKSKDELKFPEMEKKSKSYSQKEEPQNQFKQKQFVQQQPQEDDSNDLDDILDFITTENKPLPSKQSTQSSKSLEYGEYIPSIPQNRQPRNLQSSQLQEQQKKDNNNSIYDFSHLQSFKPAKMPNTNSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2D0</accession>
    <name>A0A8S1Q2D0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1390046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8109023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2D0"/>
    <dbReference type="OMA" id="NCEQMIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="581" mass="69860" checksum="D526636FE89B1C91" modified="2022-10-12" version="1">MKENLKTDTSDVIFKREATRSLKQICSSQIFFENIRPSTKQSLYQKTSQNQIRVSHYQTQIIHRNNLFIQNDKNNTIISGKTYITKAMEYSYLRICCENLIAPFGCQVQCSSEFQLMISQIHPFPTNYNCEQMINNRGFVVSVIKGEFIYISAITRKDSELQFNLLFNIQFDEIQRVKTLQDTPPSNDFLQMFKLSHSKSFQQIINTNKEQAAIKKNRYQDEISRSERFLKVLSTRNFQRQSKTQAKIQKQYQIKAKKLSNQEKILTKIIDDKKRQKIIFEQQWAIIIYLINMSSRVFNLFCHKKQIMKKNRLVAFRIKSLLRQMKKTIICSKGDTLDTRVLVDSFMSIKAKTKTIRNNLRMHQIQILLPILQQRSKIYKLKMQMLKLGDKFQIIKKHISQFEENYTKYKSDMIIKWDNLNEKIKDEEIKWKQAALRNKGLVIWFQVLKDKDLAQQMRKCFIFELMRDRIRQHIKDQQQVKKYRMDLKYYKQKLRVCRDFTEATTFRGEIFRLTNDIYAMHMKNQLFMHVDVEKYFHMLVSKYTVLIDESENLPRDLQIQQRQKQRQSRLTLRRVTRKSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4T4</accession>
    <name>A0A8S1Q4T4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GP-PDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1440055</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q4T4"/>
    <dbReference type="OMA" id="KWAIMRK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08612">
        <property type="entry name" value="GDPD_GDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052271">
        <property type="entry name" value="GDPD-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758:SF2">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="9">
        <location>
            <begin position="43"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8110448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="330" mass="39164" checksum="9F576754468B29ED" modified="2022-10-12" version="1">MNYLFYGFIGYWALSLTFFVFPYKKKNVFKHQKYQKYRQRKSIIHISHRGGCRENLENTMQAFQHAYDLGTDCLEMDLCMTKDKKIVVLHDSSLLRMCGVEAHVKEYNYADLPKFLERVRLDFAPNDYISTSTCKTPYMPLFEDVLKKFPDVLINVEIKTPDPEVVQAVNDLILKYQRQDTIIWGARFQKQNELLKSINPAIPRFFTVEGILRVYLLYMTGLLPFFDIPDDSMQAPLYTDDFYLWKLQIANSTSEKFQQMLFHNFIWFLGLISKPLFYHLHKRGIFVYYWVLNNEKEYERALKAGCHGIMTDCPTVLKQYLIKNKLYGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q515</accession>
    <name>A0A8S1Q515_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1420032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000146">
        <property type="protein sequence ID" value="CAD8109881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q515"/>
    <dbReference type="OMA" id="IMRQRNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="341"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="437"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8109881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="682" mass="82765" checksum="70EBE3CF506649A2" modified="2022-10-12" version="1">MDQQISYYQTQQFPEPNQDPHKSQRSQSNTRDLLTSPNIVYYTRTIQQQQTQPPIRLETQQLKPSQSQYNMKVQEIVKVMEPSREELEFYKNKCSLYEKQIQELTNEIQRLKSTCQEKVTYIEDSHRIQQLETNLQQYQIELNRVNILLRETTNESEQLKIKLAQINQQFMGLQQHQVEQERIKKNSQENEQYWQNEIQRLNVLVSQSQSQLQQMQKTLIETKKYEQQYIQQQQVQGQLTTELERITVLLKQKSEEYDQSKQTYIKEIEILSKRLRESELEIQDLKNREINYKQNIDNQYRDNQNLQDKYMNEMVNKNEEISKLQSIIQTLQMTLQDTTKYQEYEIRSKLQNEEINNLNQRIRMKQDELDKYKQQFIIHQNQLQEYQKYADYEIKYQNLAQEFDRVNNSLMIKLQENDQLRNCISKLQITLNDHYKVDDYENKIALQNQEIDRLHQSLQHKIEEIDRLTNEYNKMSSQLRQKNEELENLKFKLQDITQLKEYQQKFTLLSTEIERLTVQLRNKNEEIEKIRQQFSQFQLSEAHKLKELEQKNVVYSTEIERLSNLLKIKLQEIEQNKITIKQFQEECDNQKIKLINQQDVTIYNERITQLNQEVDSWKAQFINLNREYHKQQEQLILSNAELDTYKKQKNGSFKLESYEIVKENQNSKVSQYQFGTLTIRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8C8</accession>
    <name>A0A8S1Q8C8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1470094</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8C8"/>
    <dbReference type="OMA" id="RPQIKYG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002303">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="108"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8111395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="418" mass="48572" checksum="C2C0C055615C8FB4" modified="2022-10-12" version="1">MMPIQYQRQLSQKSRRTPVSTNSIYLQHLHLYISHFYILFLYIQYSRIGFTVTLTNNYRPCIKRVKLISWKHSLEIIKAQLPSLKILKDYGLTEEEKGTYGDRQLLGFDKLELLGRGGFALVWLALKGNQKIALKQIAKPQQSKEAKFSYIKSENIVQIMGVEHSNKDTWIIQELGGKPLSKVLYTMKGEFYKGERVYAISETQQLLDMYEHPQKLIELMYGILNGISCINDKHVTHFDLKPDNILVENNIPKIIDFGSAFSNEDKDQFGMITPEYMAPEALDIMHNWTKYSNQYNTQIEALTQMHGTPKIDVWSYGAIILDILHGVPNWLSYKGKIMRQGRPQIKYGLFAVKGRDLSKIIQKQQQLQLNQVIRQNCNYLSLAKQNQLLDLLKQCLAYDPSQRSEAKELLKHEFFNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9R5</accession>
    <name>A0A8S1Q9R5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1490042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9R5"/>
    <dbReference type="OMA" id="NIRYEFD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="139" mass="16675" checksum="7F6F8DFC2D08A30B" modified="2022-10-12" version="1">MASANQQFSFFQDYALKKIQFVVDNIRYEFDNHKMLWSTDVIEEDDNPDDEVNKEQLKKRGLKQIELREQVRQNEHTISKLASEIQLLNDNINKYQEKLQQSQAEQNQLIDEYIHEHLETEKIKAARERLDKLKRELGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QC55</accession>
    <name>A0A8S1QC55_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type 1 phosphatases regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1540125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QC55"/>
    <dbReference type="OMA" id="CIFHKAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011107">
        <property type="entry name" value="PPI_Ypi1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07491">
        <property type="entry name" value="PPI_Ypi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12496" checksum="92D6DE48EBA4414E" modified="2022-10-12" version="1">MISQAQTITQTSQTILHLTNSQQNEIQQQQQIKKTKKNSVQWTEDTVDNEDLGKLKSKICCIYHKPHAEESESSDTCTSDDEINAIERDKQSKLRHKMKCSKRNKKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QD03</accession>
    <name>A0A8S1QD03_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1510011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000156">
        <property type="protein sequence ID" value="CAD8112310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QD03"/>
    <dbReference type="OMA" id="YVYNCYS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="184" mass="21611" checksum="F8073BE6239E708A" modified="2022-10-12" version="1">MQTYEYSKISSNSIGMNVTLRDLLDQPIQTKTVKVIEVFISNTYEGDYVYNCYSALNISNQGEVLELYNKWPGLIEEVYIKKNDLIGIDSVLYNIKSDFRQDLQVELIQYGITNKRNQNIRINIIKKEQVLEENDPIPLDPKIILEHKDNVFRVQMNDGKMNDINFLVMKNKYPHLVIQYLEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QGQ8</accession>
    <name>A0A8S1QGQ8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1560088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QGQ8"/>
    <dbReference type="OMA" id="FFNIRTH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="38"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="170"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="522" mass="61675" checksum="802AA582135CC4FA" modified="2022-10-12" version="1">MDKQLLFAECIRILQITPLRRSPEELNLLLNFARTITFFRELGEQSVEMLQRCIFVLSYRTIKGGEVLFRVGDSGSLFYVILRGSVSVNIRLPNPEDPQQFELKEVNTLRAGIGFGELALINDSKRTATIIAKEDCVFAVMEKHHYKSILGAQEMAKIQNKISFLCSFPFFNSWSFREIKTISYHFEPVSVTLNQAIIKQNEYCNHFYVVREGEFQVQFIHKNKLFCICTLGPGESFGEEAFLNKDGQFLIKSSIFIQWAKTKYNIVCKSETGIAYKITREDIQKRFWDSKTQIVFLQLLSSIHHFRTLKAEQLIKEYEEKRQKEEEEMKFIHPGIKRSFVQKYNLLSQRNTSSEIEKDEQYDQFKLQEKQAIVQFTKLMKDRNCSYAQVRTFFDQRKFKSQNYFNKRFYHRQFFRFSNKIDEKITHDHDHNKIKTDHEIDGLLTYCTNQQNLGCNNNNSNHYPLTQVLSSPTTVDKFIKRIKNKVQIHIKNEENNTFFNIRTHSPTSHRSINSRRLKTYVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPL5</accession>
    <name>A0A8S1QPL5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1800009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000189">
        <property type="protein sequence ID" value="CAD8116755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPL5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20630" checksum="65C27439B23D0077" modified="2022-10-12" version="1">MLQIALYVPLHVQHAHLNFTVQLVKNHLQILNKVVYAKTINISILIIVMIVLQNVNNVQTLNGALNAIKVITVFILMVNAIVKMDIILLILIQFVINVQHIIVMFVIKLMEQNVILVNVVINQFKTIAFLFVVMEQKLQMNNVMMGIQQEEMDVLLVDMIAIIPVNFAIKESVQNVMMATF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QUE7</accession>
    <name>A0A8S1QUE7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T2400003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000249">
        <property type="protein sequence ID" value="CAD8118444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="QFEISWE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="2">
        <location>
            <begin position="149"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8118444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="429" mass="50299" checksum="AC02556402D29AF0" modified="2022-10-12" version="1" fragment="single">MKKQLNSLGNDIKFLRGKTVEELFYIRKWKVLKEAANKNVRSIYVPLKTLELFHKLEKQEKKFHSKGDKEGLQEEDKAGREEGDQEGNEEEGDKDGHEEGYIVGQEERGEEGYLEVDEQGQEERQIERDEEGDKGEKILMNFQNVYDKIGEANKFLLDDKETVLLIHGVARSGKSTAAKKIEEFIWKLHDTNEKIGNEILIPIYISLPSLKNPVFQAVEETLHQEEYGFDELQLKECKEFLEQKKIRFLLIMDSYDEMNLENIGKNLYINNNLNRIGQTRYLFLLQEVKFLQNKFKEIELRQFDSSQIQQYLEKFSTQSVKMLIFEIYEWQTQISNKGMIDIQQFEISWEKLQQLLKTEISGLKGENLLNQKQIDHILFFLKNDEFIALKSHDALRSLGIQFKKTMERLKVKQNNEVDQIGSVGGNSIX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMJ8</accession>
    <name>A0A8S1JMJ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMJ8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="116" mass="13789" checksum="6481B56C0AB7066B" modified="2022-10-12" version="1">MESPISKIFISDGGSLKNYDQIQNYGEIIRINEFDALKFGFKLLDDIKQKTYITFNNGIEYLQLRVFFQQKYIFGLQYIHLYCLIQEVELVYQNLTMKIDLQESQAHLQVVECFQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPA4</accession>
    <name>A0A8S1JPA4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPA4"/>
    <dbReference type="OMA" id="NTAMICF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001773">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="230" mass="25844" checksum="D6B759E9B4A1FEE4" modified="2022-10-12" version="1">MEENQNLKIILIGDSDAGKTSLIIKFKTDRFTQDSVNTVGIDLNVEKNVKINNQFYDIAIWDTAGQEIYQKMVKFSSKDANTAMICFDLSDSSSIDNVASWIQLLREEAPQNIQMVIIGTKKDLPNKYTQEQLQQIQIAWKANFNNDFPIFLTSSKTGEGIKEAFQSAFELGAKAKQDHNLCPDQQAYKSFKISTSQIQLQQNQKVIALISNTTTPKGSELNPRSESSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JX97</accession>
    <name>A0A8S1JX97_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JX97"/>
    <dbReference type="OMA" id="QQYYKEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8042838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="255" mass="30779" checksum="0548BFE24E17C9D3" modified="2022-10-12" version="1">MSILFTTEELKQIEPHPYEYVQTKTKYLEQMSSFNYTKTTQLPFISVAKAQHRQLIDKVKYYQYEEKKQLPTHQFNTIFEDQSQPPINTEDVRQQYYKEFILQPKEEEISKKDKEKNRYLHICGVINAQNQIDKKEYKNHIKELKQLQREVEQIQQQTKKPYYPTKEMCRNTNLINKLATPMLNMYNVYFKEIFDALIDDILLEEVQFLNEQQDKTLNENVQPEIPEFDNEDNQEQLNVDVKDLLLELGNIMDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYT7</accession>
    <name>A0A8S1JYT7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0100073</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JYT7"/>
    <dbReference type="OMA" id="VDCENDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8045880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="553" mass="62891" checksum="E67CCDBBAD9DA78F" modified="2022-10-12" version="1">MFACGGKSKKANQQNTILLEDKCKIHNNAFDCVSLNYTDKSQTFKACPLCKQQRTKEQFVTFKEIQNKLQTQISNDLVGEFKLKMTQLFNETEQFRTKLIQTADSIHEILVNGIHELFVNSLFKKLPNNLFTIEDIFDLVTTKTIEAFSSIMDQQIQFQKGDDTSTSLLTKILFHEQESRQFIDALKDAQGVKKKLEKINNDFTELKNEISKKIQQISKKSVIDAPKVDLQEPTLQFLIEKNKQEQDVIVEQIKTIQGVKDIIIEPSQIERQENPQVKSIISIDQKLLAYSSGTRIFIFDKTKNFSFIQQVDCENDISALCSVTLDKKTYIIASVEENEESFQLQFWDWENFDKWKVINAHDKYIWQIINLPKNLIASCSEDGTIKIFNALTQQQVQKIAVHQASVNDIALITPEIIASVSDDGTLVASNFLNGDLVGIVPQDNEIKSVIALDGIRFVTGNSSGEIRIYTFVQSIETQQVIKAHDSVVRFLSRIDQKLFVTSSWDGTHKFISVVDGEIKVINGPKKNFPEPLLWIQDIGCLVASAGGILQKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZQ2</accession>
    <name>A0A8S1JZQ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SF-assemblin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0120243</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZQ2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8047934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="28823" checksum="0C9A98D689E757AE" modified="2022-10-12" version="1">MNPNTPFNERFSKISEKLNSIQLQHDSSKAHRIDNICGRITGVEERMQDTITSYNRKLHSLKDEIVRLQKQIEEENNGFETQFEQRVREIAAFESRITTKLEQEIALRKDGNLKLSGYLDEKVVYLKSDIQTEGKIRQEQIENITTSLENDLPKLYDMVKTEGQDREDSDNGTLRRAGDEIKRLNEGLGNQKKLREESETAIFEMLKDLVSRVKSEIEEEKKLREESQESLLGLLEDACNKIYRAAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3N7</accession>
    <name>A0A8S1K3N7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GPI transamidase subunit PIG-U</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0130190</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3N7"/>
    <dbReference type="OMA" id="CFLIVQL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009600">
        <property type="entry name" value="PIG-U"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN ANCHOR BIOSYNTHESIS CLASS U PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06728">
        <property type="entry name" value="PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035918337" description="GPI transamidase subunit PIG-U" evidence="11">
        <location>
            <begin position="24"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="273"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="382"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8049023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="48059" checksum="5A1257149180C930" modified="2022-10-12" version="1">MYRIILTLKCLLWLLGKWDVDLTWLPYFSNPYTQYNNIREAKYLLANNYNPYSTDGIFQKPLIVYGLVLIDNDIIFLIADILVCFLIVQLFGEKQGYRKSILLFYHLNPIIFANIILKNTNVFDHIFLLLTIFSALKIKILSPILFGILLYLNPQYFILLAVISVIFRRESSTTLKFAIKLIVFLFVCMATICLLLYISYLITGDWQFIEKTYIDYYFPKDHRPTIGFLWALFSGLFSKYKCLYHAFFMLLPSCYILPLYNLFVKYAKREKEYLGFMIGVSLFCSFLYFQYIAISDLVIIFILLFQRYDWLFNSLPTIVIIHAGCLILFLGGGLQLTWTHSFTGNPNFTFFQVFVSYVFYVILVSEGVREMLKFRTIQKKEEKDKMKLESEDEKKENEQRNKKVKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3R1</accession>
    <name>A0A8S1K3R1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AMP-activated protein kinase glycogen-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0140265</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3R1"/>
    <dbReference type="OMA" id="INMNRCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="4">
        <location>
            <begin position="24"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="4">
        <location>
            <begin position="123"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="517"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8049988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="539" mass="63819" checksum="47B8881F8C70EC21" modified="2022-10-12" version="1">MNINMNRCQDCGNIHPRQPCAAQPVKFIWPYEGKEVLLFGSWNLFQVGTKLIGNKCTLNLAVGQYEYKFLVDNQWKYLQTQEIVHDNHGSYNNIIQVLPKRAYQIFESNAKPLIRLFEIADEVIGSWDNWSQPIKLQKRYNQFKLCDEYYTYIDLQEGRYEFIFKRGSQYFHDSCQPTIDNSFGKKNNIMVVLINKTESQELDFNNVYWTKHDLLYHTFDHIYGHSMTSIGNSFYIFGGAPSRNEMYKLTFGDHQLNLEETEGEIPRPRAYHNALAYGDKILFFGGVDEHNILNDHFVYVTSAKTWYLAKTDKKWTERERASLTFYAQEELVILFGGYYLSPDLEVELIYNDVYYMNIQQMQWVKLNINNQPQPRYGHTAIQVNEKMYIFCGKNQDEYFNDIWMLNFENVQWQQIQTQGIAPEPRYGHTTNLIKSKICIFGGRNSKSNRLNDLHLFDFITNTWITPTQYGLMPSPRYFHAADIYNGEQLWILGGNIGLKRNEHFYIMNFGKQEEQTQVQQQIQQQQQQQQQQQQQQQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3V6</accession>
    <name>A0A8S1K3V6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0130431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3V6"/>
    <dbReference type="OMA" id="ICTICQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09633">
        <property type="entry name" value="Deltex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039396">
        <property type="entry name" value="Deltex_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039398">
        <property type="entry name" value="Deltex_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12622">
        <property type="entry name" value="DELTEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18102">
        <property type="entry name" value="DTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="142"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8049371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="332" mass="38806" checksum="FFBC08F7D3BB3C8F" modified="2022-10-12" version="1">MRQFGRKEQKQKSLSLPFDQLIKRLNELKVLKNNYIKSLIPNNNDQKKQDDDLLITKNKNSQIQNNIKYDAPILHIIDKQYDLSKSNFQQEIIQSINKLNIEIDNQASHLVNLRHMSSIEFYLLVSQYHLNDNLHFSKSDICTICQFELFEIADWDKFEEILCCEQLVVGLPCDHYYHMDCLTHCIKEQYIQCPICGKIYGVKTGDQPDGEMICHIDYRTHCQGYEDYGTIVIYYNMYAGQRNGQHFPSTYREAFLPDTPEGRQVFRLLKMAFERKLIFTVGRSVTTGKDNRIVWNGIHHKTNLSGGVSCFGYPDPTYFSRVKEELAAKGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KC75</accession>
    <name>A0A8S1KC75_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200305</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KC75"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="127" mass="15077" checksum="867074C09A851E4A" modified="2022-10-12" version="1">MQNNPIKNRQVGFIIAYTLFQDSMVIIINPQKDKQSTDLRNTVHFRDIWIGIIKQIQRMNLQINWKRELLITYDDGITSIFRNNQNYQFIIHVSFKMTAQEMSTIEKKILDEEILIQTGLQNFTINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCJ8</accession>
    <name>A0A8S1KCJ8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0130010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCJ8"/>
    <dbReference type="OMA" id="HQIIVWK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="271"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="548" mass="64759" checksum="BB7326874271AECE" modified="2022-10-12" version="1">MQQTNMLSKIFICVNKDGQAECINYDSMLQGDTIQLLCKGCYEKKSLENSKALHQEKAFNEAQQQFNLVWNRIETKINKISELVQQAQVFRSQFLSFFDQFFSYLDQWQKSLANLNEKSKTIYQLNNFNTKLIQQQEKLFNSSNQIYITKLCNRFREFNLNCQREQQKFSILLTQLIKETQNVVPNQIQNVVSNQIQNIVPNQIQNVVPDQTQNVVSDQKIELKIDSIVPQQECCLNIAFNSSNSIMISTQVFNILTWTFNMGKIKTQQSLIKHKSWVNCIVYSKEKEVFFSGSDDKTIIIWQLQENGWRSSKPFEKHQVNCLLLNQKEDQLFSCGNNHQIIVWKINLEMNELIYQYELNKHNSKVFSMSLNQSESTLVSCGDQKHEIIVWEKGKDNKMKFKYFVKQQQSQNYFGKKLFFLSEKIFIWVTATKEVDKIFVFEIKNEVFQENQQKTIELIQEKEYDDMFLFPIIREKNSNMIIVRHKTHIYFLKDLGLGKLKIAQQINCETTNIYGTITNNLSHLLYWDNKTGGYKIYQIKQTEQFLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEC0</accession>
    <name>A0A8S1KEC0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210242</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEC0"/>
    <dbReference type="OMA" id="FTLCEDA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="276" mass="32871" checksum="57BDCF487E0F3ABA" modified="2022-10-12" version="1">MDQTQYSIHQILQNKRDLLRETIRNNDLEKIFQQKRIQTQQNQESEEVSILSSSSSINYRKTQSIIEIESSSDEASLIRLTKIRTKANLTPNYLISVNEYLKRDPYIFQVQKIFTLCEDAILDIKLTSSDLVPYLVEIIYSVTKYKTQIPFQPLTLLLQAFSLHQDYIILQYIKMYLQNNKQETNEIDQFCRKKCFFEDESFLFIICNNIKYANLQMLLFLCETIKNNEFIQAIKVYGAKIFNKNIEHKKEYRKIIKEIQILAEIRLSNKEILYQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KGL4</accession>
    <name>A0A8S1KGL4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KGL4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="207" mass="24689" checksum="FBF2BEC180BFF4B9" modified="2022-10-12" version="1">MGQKNQKATPVQTREPIEIKFDLKSIWKLLNTEFKKNQEEKDKYISKAKQALKYNDDEVAKQFLQIALNKIKVSQNFLRMANRVLFLLQEVKKSTQTNVDLMQQLNVVTPILLQYAKNMQIEQSQQNINEIDRTLDDIMVQCKVMDPMMSKYDDNFQTNLTVDYMIQQLKKEEAQQLQQLQPQSMTFQQVQQQQQQQQQYKNQEQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KID7</accession>
    <name>A0A8S1KID7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KID7"/>
    <dbReference type="OMA" id="RPIKIWN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="254" mass="29809" checksum="5A795F41BADAB624" modified="2022-10-12" version="1">MKSSRKQPQKMKAFNILHLEHVANRIDQLLNPTYREQFTQVRQIKPGIKFVINNKLTPYKHRQSVFEQIQSSRSSIIQSQDRQRKQNNNSSKSSSFLEKKINQLKKELGDLQKARLFEKNKPIHSVHKMALYNNLQEMQQDHKLLIIDKKPQSARKIVDKSIEEFNGPIKKKPQQHMFLQNLVIENSSNQLEPSVISIPTPEIKAKIKVIPSQQRSVSQGLITQQKRPIKIWNNDLIEFKGWDVEEEDDYLRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIP2</accession>
    <name>A0A8S1KIP2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0190341</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KIP2"/>
    <dbReference type="OMA" id="RHVSENN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051942">
        <property type="entry name" value="DENN_domain_containing_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288:SF0">
        <property type="entry name" value="UDENN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="cDENN" evidence="2">
        <location>
            <begin position="214"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8052732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="479" mass="55794" checksum="3AB48FF3EA6C0565" modified="2022-10-12" version="1">MQNNDPIPLYMLTKKTNVDHLVGSKASRTHTRVETKVSTSISNSQNSQRKTSFYNRPPLSTKNTFSVHVDTDEGGGVKKIQSYQNINNKMEKFSQQNRHLICKFYEGIYLIGNSTIGKTEILYQYGQCHLEQLLPDLVFPYGAKDVQKKLSDSQSEINEILFKRHVSENNPNCFILPIKTSESFEKAQYNSNQLAYCICLSIQDICPSKNSYLKCERVLCIVSYLPLIKYFIDFLIEVMNCAKEERFNNLTFTQYIQKCPLFETLPIQIPSQQIPQSYYDFHDYHVKQLKILNNALSIGLVAINIILERSVIFVAQNVQQLGAILMLFLKHVKWIHQMVASIPPKNIELLNVPVPLLGGLVLDDYQCVEQLIDEYPNVVFVDLFNQHVRNSHDFTKSLIYIFLVRQLQGKSYYEMGQILGQFQQNLVQENSRDEYIIKEFRQTQYFHEMDQLKQSLNVRNIPTKSHHIKVQQEQTFRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLP1</accession>
    <name>A0A8S1KLP1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLP1"/>
    <dbReference type="OMA" id="HANSTHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052258">
        <property type="entry name" value="Diverse_Func_Domain-Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37612">
        <property type="entry name" value="FIBROIN HEAVY CHAIN FIB-H LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37612:SF20">
        <property type="entry name" value="PER-HEXAMER REPEAT PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="362" mass="38068" checksum="42306286BC6E47C2" modified="2022-10-12" version="1">MIILLLILIPTLLSDNIQLARVQYCGCGVALIQSLCIQLDDCRWQNFKCVNITGVPQTIVVTPNNTNNNDNSTDGNSTTGNKTNSTQDEYDFTDPDFDYDSVVVTHKQYDIPDIIEEEVNTDEGGNTDEGGNTDEGTDPGTGTDTGTGTGTDTGTGTDTGTGTDTGTGTGTDTGTGTGTGTDSGTGTDTGTDTGTDTGTDTGTDTGTDTGTDTGTDTGTDTGTDTGTDTDTDTGTDTGTDTGTDTGTDTGNNNTNTNTTQTFTQSTSITYTKTYINKTFNDKVTCSQQSDEAGCNSKRWPDQIFCIWKNSTCISGSCNDLDLIECEKTFFCVNFGYCQSCKAIEEYAQTLLVQILILAFLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KMG4</accession>
    <name>A0A8S1KMG4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">R3H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0210328</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KMG4"/>
    <dbReference type="OMA" id="TRTVCEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18044">
        <property type="entry name" value="DEXXQc_SMUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02325">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H" evidence="8">
        <location>
            <begin position="708"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="881"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="977"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="159"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="319"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="889"/>
            <end position="905"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8054162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1006" mass="118026" checksum="DF08353ACB9FEB09" modified="2022-10-12" version="1">MIKQTQKVYESFEHFCGLQQQWIKQEHEEEQKFIQLISQELTHAQIIQMGYGLGKLKLVKTKLTFGNRQVLVFEQQQKLQQEDNKEEQNKEFSEIKQENFRLKQGDQVGIYIWSNVLNQQKFKNPEAIGVIDRYRKNLRIILEQNCKVPDDNSIYGICLQENQVTYKRHLQVIEDLKQQYEKHPIIQILFCDQQEEKIKEKIDQNIEFQGDTIQQLNEQQKIAVQKAMKQPHISLIHGPPGTGKTRTVCEYIKQAVLVQRQKVLACANSNIAVDNMIERILSISQLKVCRIGNPARMTDQVRYVCIDQLVKKTTSYAIMKTIKTEIQQIEKKLNRTERKEAAELRDKLRQKKKEYFEQQQLAYEEAIQDCQIIFSTNVGSGQFQFQQLTKNIKFDVVVIDECAQSLEISCWIPILKGNKVVLAGDHCQLPPTVKTKNTGLEMTLFERVENELHINTQLTVQYRMNKSIMQWSSQEFYEGHLIADQTVANRTVSDTILLFVDTSCCDFYEQQQESLLNFDDQNKSKYNVGEAGLVQIISEELLNLGVQKQDIGIITPYNAQVQFLKSKINDIEISTVDGFQGREKDCIIISMVRSNQIGEVGFLCESRRMNVAITRAKKFVCLIGDSETVSKDPFLKRLVDYFVENGEQRSAQEYQHDDKIQIPIKIEQVQQDVKKKKNLQPKIQVKEKNKNNKIEDQQIAPNIVQQEQNVLSQLEKQVLDFINSNESELIFKGQNSFQRKELHELAEKYNLNHESIGNNKLKTLILSKIQNGNQSNLQQNDEDKIEIIDNNKSNNQIIDDAKADKSQKVEKQLQQQQQQQQQQQQQQQQQQKQQQQQQQQQQQQQQQQQQKQQKQQSQQQQQQQQQQQKQLQQQQQCQQKQPNQNSQQKQEKKQNTQNKKTKEQLEKEEDEFFDSIVLKDEKKCSFMSNNIQCSQSILWLSQKCQFCNNQFCQYHSYAERHGCEDEACRHAKLQFQSNERKTEKSRTALQEKIQEQQKSRGKKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KNA7</accession>
    <name>A0A8S1KNA7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transmembrane protein 107</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0250076</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KNA7"/>
    <dbReference type="OMA" id="VCLKKVP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036038">
        <property type="term" value="C:MKS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904491">
        <property type="term" value="P:protein localization to ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029248">
        <property type="entry name" value="TMEM107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34341">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34341:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14995">
        <property type="entry name" value="TMEM107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8056830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="152" mass="17195" checksum="BD26056A9A639812" modified="2022-10-12" version="1">MTINGLLIPSKFILVSCHFITSLMAYYGAKENILANFQTKTYDTTSDTYTSAYNSIVSCIILGLIGLGIEMIFIITGITMFYDTSNFLIICLHAVGIIIYSEFIIGAWPYYELWYYWAAFILLPVLLEIVATCFGNILYGTAFKRRLSRKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KP48</accession>
    <name>A0A8S1KP48_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0250206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KP48"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="53" mass="6416" checksum="18A30AB092169F74" modified="2022-10-12" version="1">MAHAVSRTLLSIFVIIRFTFIHLEIVYIMISFDFIIVFLCIQIDRRRKVQQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KS41</accession>
    <name>A0A8S1KS41_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KS41"/>
    <dbReference type="OMA" id="DESIMVW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="149" mass="17112" checksum="A887D48D20D27ACE" modified="2022-10-12" version="1">MQNRLTKELQDLTTGNPLAGVQITPDPNNRLLWTAIVAGPEGTAYQGGKFKLSITFPENYPFKAPFFQFQTKIFHPNINEKGEFCEDMIETKEQWQPTKTVKQVLEKILNILGQVNTDQSLNNQAMEMYKSDKNKFEEFVRAETKKHAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KS88</accession>
    <name>A0A8S1KS88_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KS88"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="200" mass="24003" checksum="174812A0B79E44D8" modified="2022-10-12" version="1">MQQQSSFNNTCIKNLISNNCNCQIHECIKQIINLKSQQFNNLNKLLNLFASPYKICDQHHYPFEFFSEEENDMKCKFCLDRNSKLQLIQNMTNQETQIDLNQGLIDFPNSSDYIENSFKKLWNQKSEELQKDLENTTEIEKKWMERKKIKETIEFLKMTYPKEVHNEFHDAMTKINQIDQVGSNFSNYDVLKYRDLLLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KT06</accession>
    <name>A0A8S1KT06_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0270007</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KT06"/>
    <dbReference type="OMA" id="VAIQPEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="31"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8058037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12625" checksum="01FE07BFA923C8F0" modified="2022-10-12" version="1">MMLRFALRKLNKCQEAIECYDKAISINPKYDKAWYNKGYVLHQLQKYNDAISCYDQALSININPLRLQRKADSLFELGKKSEAKQLYLSALEKGSNDKNYIQKQLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWC0</accession>
    <name>A0A8S1KWC0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0270088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWC0"/>
    <dbReference type="OMA" id="NMREPKT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="292" mass="33648" checksum="023AC14815890C94" modified="2022-10-12" version="1">MKDFTKFVLCALGGTIVGLLYKLNKERINKNEIELEILRNQKSISVQQLKQSFSITDDNQLNLFVEGQIAQTSEILKSTNSKHAGVYIKRSNYELEIVENNGNKTQQRRQISKTINTTDSFILCSQQNSNEKLLIKNFSETKILPKVLKYQQTDQLLIPPEVRTIMREPETDEERAQFKEGKLKKLLTTISKIGVVIEEDILCEGTFICVYGNVIWDRVQNSFRITAPKFFGISKEQIIGYFEADQYYLKFIGILLAIGGTTFSIFALKHLYKTLFNNQNKQETKYQTKTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWG7</accession>
    <name>A0A8S1KWG7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AGC-kinase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0260166</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWG7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8057682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="40" mass="4874" checksum="E89C0DDC09281E9D" modified="2022-10-12" version="1">MSKRQVEPPFRPQLEGKKDLRYLDPDLLQQEIKFEEELDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9D3</accession>
    <name>A0A8S1L9D3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0330064</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a fundamental role in microtubule organizing center structure and function. Component of the infraciliary lattice (ICL) and the ciliary basal bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the centrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9D3"/>
    <dbReference type="OMA" id="RKPQDNG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="70"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="106"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="218"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8062293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="270" mass="31234" checksum="F372ED9E9D30F2C5" modified="2022-10-12" version="1">MKKQQQQSKSLSSRKPQDNGKSVKMQEKLTKIQEAFNFNKASKYIFQSGFQERAWLIKRGYQDRIDFKDSEIAELRKYFSSLDGDGSGAIGIEELEDPLIALGLVNSREEVEKIMSEVDEDGTNEIEFKEFLTIMRGVQKGGNVDQGEKNPIYDFFKKMSNGQLEKGMDKHIPFKLNVSLFRRKQILNAITGDQKELKDKGQKILQAYKRQLLNYKQQDRINRGEDPNDISLDQFPNKDNGPNPRNGFPKLQRVIQPEQTIGNNHKHYDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9K0</accession>
    <name>A0A8S1L9K0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0330099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9K0"/>
    <dbReference type="OMA" id="SMTDEHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="169"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8062363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="567" mass="68405" checksum="52F5C60337C8835E" modified="2022-10-12" version="1">MSKTPNRLVLPELENKSFRDSKQIQDLRRKLEQQKDTIKELVNLVKESKTEQFYKPQFNQSIVQANQEKDEVTLQLLEEVKTLRRQINNIEQGPKQSVQHPIIIPQYLPGPPQQQQQVQPMMSPYMFMNPYFPMPPPFMYPYQQSQQSNQDDTYKKIVLEMLQKSNKNIKDINRKRRDSYSSEYTDDHDDNRRQHRHHSNADGKYYQNNRQNQRRNKDKKQIKDRNHKDQDYQDNLRTNSRISINDSQMSYNQKLNGKGKRKINQKIFTQDEKLKLKRKLSGLFWYIRIGLVLRKYLKRVWLQRRQEYYNTEAQQLIDKFDHEFNYKEVFVLFVVECNKNKYFTKNWNIFDNKEVELKSKLIFSIINILFKKIPFMTKSSMTDEHKQFIKKISTPGGYLLPGHPQFVTDRVELRPNVTIGQINNEVSKLIQMDYIYIQILVQRVILIHEWYSQYSKIPNYKEAMKIFVTIMHQLFIDHFINLPTYENLDSIFNQKQVVYCDFTQQNYIDVAISIDDKHPKYQPTKHCCILGLGSNDEMRPLYEQPGYKDLQIQFKEYCEYFYQSLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBJ0</accession>
    <name>A0A8S1LBJ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0350157</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HENMTIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF530">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE DDB_G0271350-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="49"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="119"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8063821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="193" mass="22978" checksum="7AB559B67CC038DF" modified="2022-10-12" version="1">MQRXIVENLKIFHLLLLKELVREIYIICESQENLVSRFPFYYQTFEFNFPNEIIENQLYLGNSNHANNLKALNLLGITHVVNCAKEIPNYFNDVKQENKNFIKYFQVPVLDLEEEPISDHFDEAYIFINDALSQQENKVLVHCAQGKSRSATIVLMFLMRNKQWTFDQTYEYVQKRRETISINDGFQFQLLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFS9</accession>
    <name>A0A8S1LFS9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein uL15/eL18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0360109</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="LFMCKVN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="3">
        <location>
            <begin position="2"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8064403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="211" mass="24486" checksum="26CA812BA7938D9E" modified="2022-10-12" version="1">MGVDAYKKTRVQRNVQRKVTSTNLYLKLLIKLYKFLARRTDSNFNATILRRLQQTRTARYPISVSRLVKQINTAKDKTRTLVVVGTVTDDVRLLTVPKLNVCALRFTETARKRILAAGGKTLTFDQLAQQNPTGTGTILLRGPRVREELKHXCFQLYNLHKQLDQYHISTSNNNNIMCFLQQMIKSNLLDLQIHLNYNQQVRQTNQLFQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LH57</accession>
    <name>A0A8S1LH57_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0380134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LH57"/>
    <dbReference type="OMA" id="HERKNTD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="295" mass="34717" checksum="568E897D4AB97E2A" modified="2022-10-12" version="1">MRSPSLFSPSKQQRYEKQNGSFQTFSTTNQVNKAQLADFRKEIRNNISQREPFIPQQENKPILLTNLKKHERKNTEGNVAKYMARSSPKKSLPGGLNVEEIFSQKKFIDKNQSQNTFYYQQQRKVDQLFKKDQNDSFSKQDNSFSNLFKPKRSSQAHLSRQSGRDQSPINLERFRSWDTKLDLQLKQLNNTKDLSFYKYSQQPQKENIYETSKPIQTKDIFNINKNANNFNKFLKNSSPPKFIEKIPLTEIVKMQSYFSQLPTVEITTIPRGYLQELQNLQQTLARVVRQSQQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIH9</accession>
    <name>A0A8S1LIH9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0410008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP97 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIH9"/>
    <dbReference type="OMA" id="LYQNPIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038791">
        <property type="entry name" value="Cfap97/Hemingway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029488">
        <property type="entry name" value="Hmw/CFAP97"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23035">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 97-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23035:SF2">
        <property type="entry name" value="KIAA1430 HOMOLOGUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13879">
        <property type="entry name" value="Hmw_CFAP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="197" mass="23385" checksum="FD08A34A213C8E86" modified="2022-10-12" version="1">MWNLLYQNPICFKKKTAQDQLKHITTIQTAKSCLHLQPPWQSTHSLQKLSNKYTHIQDLDIVNQNHILMSKMKEIQKKKTSFKIPTSYASIDNIRKKETTKIIQENQTLLKRLQSAHSQYPVKFLKKDNERLKQYRENLQKTKSISQNNKGTYNDFNVLAQQEFQRKLLYSSQSQRKFLQTSERNRQNTCQTVPSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJJ4</accession>
    <name>A0A8S1LJJ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0410237</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJJ4"/>
    <dbReference type="OMA" id="VSERTHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000636">
        <property type="entry name" value="ATP-binding cassette sub-family D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF67">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY D MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="81"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="434"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8067930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="652" mass="74697" checksum="D51E0DB6ACFA9063" modified="2022-10-12" version="1">MPSVLSKIPLPQSFQNKALSGALLVLILMIVKSKKEASSPRKIPRRQGQGNVDKVFINRILDLLKIVIPSIKSMEVFDLSVLTVFLFIRTMMSIYISSLNGRIVKTIINGDLQKFIKKIVQLGLVAIPASFVNSYLEYLNKSLSLRFRRRLTEYFHDQYIKNMIFYQVSNLDSRVTNPDQRLTTDIEKWANSLSQIYSNFSKPILDIFLFSKKLAVSIGYQGPMYVFLWYFFSGVLLKLVSPAFGKLTAIDQKLEGEYRSVHSQLLYHAEEVAFLQGQNWEKLKINQSYQQLNKHQIVVNKLRLFMGIFDGLLVKYGAVMAGYGIMGLPVFGPNREEYLKKIGNDKSAITRDYVSNSSLLINLAKAIGRLVVSYKEIQQLAGYTRVVYEIKEVLSDLTSGYYKRTQVQQENETGQVVIDQGKHQTIQLDKGQIIQTDDIIEFENVPIISPNGDTLIKGMNFKIIPGMNVIISGPNGCGKSSLFRILGALWPVQGGTLYRPAIDKLFYIPQRPYLPPGTLRDQIIYPHTKLQMLRRKINDQDLIKLLEDVQLEYLVYRERGLDAANDWNDVLSGGEKQRIAMARLFYHKPQYAILDECTSSVSMDIEAIMYNKAKALNITLFTVSHRQSLFKFHEYILKFDGEGRYWFNKQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSP4</accession>
    <name>A0A8S1LSP4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAD assembly factor SdhE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0410139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSP4"/>
    <dbReference type="OMA" id="KMLMHRC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005631">
        <property type="entry name" value="SDH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03937">
        <property type="entry name" value="Sdh5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="13782" checksum="C74B5DDF2E3864C2" modified="2022-10-12" version="1">MASKNFISKRVAEQFTKNIILPNRPKDLYSYQKMLMHRCTNLGMKELDIILGRWSQEFVPKMNYEELNQMESEILDLDTIDIYNLLLQVKVAEELKPLEQLKYVQEIRNYALKGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LT13</accession>
    <name>A0A8S1LT13_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0450108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LT13"/>
    <dbReference type="OMA" id="DYQKFLC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="31429" checksum="AE86E4690E383512" modified="2022-10-12" version="1">MLIIDKIIAIWQSITFGTKFVLISSILCCILDILNNNLLFNILVDIPNLTIYQYELWRLLIPQFFHGNIYNLSISLLGFLVCAIEVEKNYGTIPFFCDIFFKNLIIQIIYVFLCFSLSYLTLDVQGTQSFGFWNITFIYMLNKGLADQDDYQKFLCFPLYFPSKYYPPAFFLIMNSIEYPRLDLIAATLFAFIEYQLFDGFMMKLSKGFVTKIEQSFPFKYIQSRQDFITCDQINHSFVMDDAKMAEYQLEIGMISTTESKIQDQNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXK1</accession>
    <name>A0A8S1LXK1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP synthase mitochondrial F1 complex assembly factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0490017</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXK1"/>
    <dbReference type="OMA" id="RCEIKDE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010591">
        <property type="entry name" value="ATP11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126:SF0">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126">
        <property type="entry name" value="CHAPERONE ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06644">
        <property type="entry name" value="ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8072227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="22855" checksum="A65E1220693FE268" modified="2022-10-12" version="1">MLNSFLNKYKFAFTYPCPRKLREIVKMSLFERESKDQVISLWMEYHKEKQHNVAYIVSKDEYEILKRNTKESPLFLLPIKRKGGHFQLIGQSQTNSILFTFLEEYKKSGSFSTPYFILTIFDELLASKQLALIRGDIMDYKIDKDEATFLTNQFLKFYMTPELYEKYIYTLNHKQHEFNYDDFRNHFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYP8</accession>
    <name>A0A8S1LYP8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0510293</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYP8"/>
    <dbReference type="OMA" id="CESKHIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="453"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="115"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="149"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="207"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="241"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="275"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="309"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="343"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8073888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="58264" checksum="9FC15932F80BF6C8" modified="2022-10-12" version="1">MQQEINDNSKFKSLSITLDQVQIQLNKEQNHDETIKKELDQLEFRKEIQLKNDDLNNINIQQIKLEKSKKKNDLKQRDKAFQEYFQKGLQLTRLQNYKEAIEMYNKVIIINPKYYPALNNKGLLLSRLNQYEEALIYFEETILLQPNNHQVYNNQGLALTKLKRYEEAINSFNQAIFLNPNNWKAFLNKDQSLECYDKAIELNPHSDEAYYNKGSLLRKLDRYEEALEYLNKAIQINHKSDIALTNKGLTLIKLNQWEAAIQIFDQALKINSKNYKAVLNKGFALLNLFQYDQAGEQFNQALKLNPNYDKAYLNKGVLLNRLSQFQEAIENFDKAIAINSKYAEAYFNKGIALQSQELHQEAITLFDIALQISPKLNFKRQKGFTYHDKISHFFIFCKQVLYGQILYARIRILLKRYNNISKKQKMRQFKYSQQQQQFYNKQFVFLQKLIKNLPLFLVNLVFLSGFTILFKNSLLNKDPFQQQLEKFDQLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZ64</accession>
    <name>A0A8S1LZ64_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA topoisomerase 2</fullName>
            <ecNumber evidence="8">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0500035</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7 8">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the type II topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZ64"/>
    <dbReference type="OMA" id="TWTQDFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03481">
        <property type="entry name" value="TopoIIA_Trans_ScTopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03365">
        <property type="entry name" value="TOPRIM_TopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.268.10:FF:000018">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000030">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.30:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000008">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000097">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031660">
        <property type="entry name" value="TOPRIM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034157">
        <property type="entry name" value="TOPRIM_TopoII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF38">
        <property type="entry name" value="DNA TOPOISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16898">
        <property type="entry name" value="TOPRIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="10">
        <location>
            <begin position="430"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="11">
        <location>
            <begin position="675"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1058"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1166"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1217"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1065"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1184"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1223"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1265"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="7">
        <location>
            <position position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8072807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1274" mass="148123" checksum="56F1B68D7FC582B3" modified="2022-10-12" version="1">MRQKTIEEQYKKMTHIEHILQRPDTYIGSLQRTSEQMWIIQNGKMIQKEIEYVPGFFKIFDEILVNAADNLQRDSKQFKQSYIKVDIGNEISIKNDGFPIPVEIHKEYQIYVPELIFGVFLTGSNFDDTEKRVVGGRNGYGAKLTNVYSTEFTLEVCDGKSYFKLVWNNNMSNKQIPIIKQVKKDPYVSISYKPDYKRFGMKEIEADTQALLTRRVYDLAGIYGNRITVYLNDEKIKINSFQKYVDLYLPNEGAIKIFDKEMTTPRWEVVVSYSPTQFQHVSFVNAIYTAKGGTHVNYVTDKIIQEIQNEMNNNKKYKSIEVQKYQIKQSLWVFINCLIDNPTFDSQTKENMTTKVSEFGGTNEEKFKVTEKFSKALIKTDIIETIFQQAKAKADAKLNKQLKGTKTGRLHGIEKLDDANDAGKKNSELCTLILTEGDSAKALAMAGIDIVGRDRYGVFPLKGKLLNVREASLKQILQNEEIENLIKIIGLQKERQYNDLKSLRYGSVMIMTDQDIDGSHIKGLIINFIHHFWPSLVKYRGFLKEFVTPLIKATKGNQTIPFFTVQDFNKFAQDEDIKTWKIKYYKGLGTSDDQEAQEYFKNLRTHTIQFRYDGDEDDNSIDLCFNKKKANDRKQWLAQYNHDLYVDHTKKELGYSEFIHKELIHFSMADNIRSIPSLMDGLKPGQRKVLFACFKRNLKQEIKVVQLGGYIAEHSAYHHGDLSLVSTIIGMAQNFVGSNNINLLLPKGQFGSRAMGGKDHASARYISTALNKITRYLFPEQDDHLLKYLEDDGQMVEPEYYVPIIPMSLVNGAEGIGTGWSTSIQNYNPIDIVQQIKNRLDGQQFQPMTPFYRNFDGIIENLPNGNGIIKGLIDCNQETDIITIRELPIKKWTKNYKEWLDKEMAEEGSQIVDLREYHTKYKIHFEIQMVDGFVQDLKNPLEYFKLTSPASCSNMVLFDSNNKIKKYECVQDIMEEFYLVRLEFYHKRKDYLISKLDREVQLLDNKLKFIKMVISEEIKLRNIKKIDLVKQLDKYGFTRFSQLIQVKSTKVKAFGDSQKQQKNVDDDESEVEEVSGDEEKQIKKQISKKLQQIPSIDISEFNYLLSMPLFSLTYEKVEKLQDELNQRVQSREALINKEISVMWREDLDKFLEAYEEMNEIELRLINQQEKMPGKQMKKPKKKNDKQEQKIVEEKVQKSKAIKSKDPVEEILSKYKKQDDKLSEINSKQNDINSKQLNSKQQESKLQIDNSKQVSQSIQGRRIRRLKVESSSDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZK5</accession>
    <name>A0A8S1LZK5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0410285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8068024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZK5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8068024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="475" mass="56506" checksum="8BD2C3BD5262C13F" modified="2022-10-12" version="1">MGQIFQKHATQFEPSLIVKLKSITMRELFDCVDTIDKYYPQNCYLNLEQFDDIFATLTDNCIDLFHRLADQTGEDGKLSVHTFEALAAFAIFSGESFETKCMFVFRLFDFDLSNTLEEQEMVSTLQCAVRAMCKIAGLVIPSVSMLEKLGYVCFQMMDEDKNKHVDFDEFYEWTVQYDELQEFMLLYSGTQTYQYALKRMINVMDESEQQFNKWLRECKAPQVPAIKLQQNLYQIYKGIDQLSIDTLFYMIIEYTLRSKSIGQEDSSQQLKDLWAWKADHKKNQHLLQNISIKKSDYLDIIRAWNVYSITDVNQLSIINIKDLKLLIWLYEDEEPDYFRINQEMSIIDTNHNNIIERCEWMQYLCSEESMQRRQTPQVLMKKVFDNYDEEQCGLIPIIHIDKLLRDTFKQQMKKMKDFKSIQSVNSLIQQLKVEFMKQVKKNNQSHFDWQQYYQFLVEATKTHSSLNKLLNLNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M108</accession>
    <name>A0A8S1M108_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0510083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M108"/>
    <dbReference type="OMA" id="CFRNQKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="108" mass="12925" checksum="547E5C6FAC853879" modified="2022-10-12" version="1">MIQIKGPKLNFNMPIKIQDLMEEKPYSQFDLESTDCFRNQKISKYVKFAPTTTIFLRYQDEDIYRFRDRLKTQVQITKETFNLNPTLEDSPKKQQLKSCMKIAIDSDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5Y5</accession>
    <name>A0A8S1M5Y5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0540240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5Y5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="526" mass="61783" checksum="9F8A4AD55EB1969A" modified="2022-10-12" version="1">MQNKSYPSTHYTKSSLSKQSIHQQVKPQKSSFTRWLQRLFCIKEINEQQYITNMMEISNYDESQQPTMKIISKFSQYDEYFSIQDPIRIISGSDSQFPFVVKFNEEKLCEIDDQYHQQIPELNGQVVVLITQNPPLALINYIESQASYFIIYLSTPNPIIQDYIQQRSKQYSKPELIIGKFSIKKSLSLVDQYIFMPDYSTDGRQQVVDTVSQLLKLIPNEIDKFTFLIKLQGNPIQDIFSISSQMNQAFIQKYESEFWINKILRLTQNRNAIVLKQRDQKKTGFEKSQYMMLSNNINQKKGYNLTVENTELIMNLTKYQQGNVITHILKCLIYQKGIERRNNNTIDQAKLFCTLIFLIKELKRFSQYDQEDFISLMKIIQQSSFLSCCLCINLMKQEDLKELSQIKMHYKRYLEILICSGIITNEQDSYILQTILNKCLNCPIYDISIPKSEHLNSGENYTQQLIQNEDMGDKIYSIDYDFAKSPKSYQIILGLQQDLNYSELQIEPQKRFPQQTTTSISNSLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8Q1</accession>
    <name>A0A8S1M8Q1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0520046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8Q1"/>
    <dbReference type="OMA" id="HTQNTKT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="151" mass="17816" checksum="17B62C0A011F7E01" modified="2022-10-12" version="1">MRSESINRRKQVEQQQLGEENQRLKIKLLEIANEKKALSQQVKLCQAKKKLSELFRHQQNMMENLILMKKITNVTSSITHRVQLQDFHFSKLKQLDRYLQDDEKSFVKPSIIQSPNHSQQNSQHTQHTKTLDATHIKLSELRVYSNGQLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M959</accession>
    <name>A0A8S1M959_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0520269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M959"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="197" mass="23866" checksum="3FE03A887EA1EF24" modified="2022-10-12" version="1">MLRELRMDSDLDDLIKSLMDYSQDIILQIISQYLQGQFQQEITKNFNSHQVEYFLQKCQQTPIYEGFQKFGQNGQIQDLFIFLKENVGQLEQQLREENSYSYYLMHKRQKDSYDEHFHLNLNLPQINQQEERKLSVDSILLENKKEINFIDKYHVKVLQKYSNEDYEKLFMTVYDQCGKFCDEHKKAKLHGYYTVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9F0</accession>
    <name>A0A8S1M9F0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0560098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9F0"/>
    <dbReference type="OMA" id="ISIVIWR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02208">
        <property type="entry name" value="cupin_RmlC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43212">
        <property type="entry name" value="QUERCETIN 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43212:SF3">
        <property type="entry name" value="QUERCETIN 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="214" mass="25438" checksum="0E7678AE4E139985" modified="2022-10-12" version="1">MLQNLIQEFVHTQFTLRPQLYQRCGFEARYFFNFGEHCDPRHQLPISIVIWRLPPGEQLKQATHSQNDIITYILKGQYRQDNSISENQSFKEGDAIQYICGNDNSYHRDSNNSQDVSEILQIFIQNIDVQRDTYQQIYKQNQIIRNEKYYEMCILEGDSIIDNTNFTFLIIINLDEALLTIDEKQNLRKYECQYFNTLSKIKISGNKYIVIKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MB69</accession>
    <name>A0A8S1MB69_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0570117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MB69"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="249" mass="28305" checksum="1212FA2F60A78F7B" modified="2022-10-12" version="1">MSFYPGYRYPYAAPLTNSIAAPITYAPQMSYAPQVNYAPQISYAAPVQYAPQMSYAAPVQYTRPITQSVVQPVVTQPVAVQQVIQQPVIAQPVVAQPVQAPIKGESRVEYREYQRQVVEMETETVQVQVPKTKYVTDYYPIEYQTEYIPRTVYEQQTEYVPVTKTVPRVEYEAYEREVQRPQQVVVQQPVVQQVVQQPVVQSVMQQPIVQQPLSYSLVRQAPTYAAPMAYQSVAPVGSYPGFYGGYRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MD98</accession>
    <name>A0A8S1MD98_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0540269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MD98"/>
    <dbReference type="OMA" id="PQQHCQG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="152" mass="18164" checksum="B75B5D7DA30EDDC9" modified="2022-10-12" version="1">MINQDSADLDIQQEGFDFQKLFEKKCSNQETRKEEIHKYHQSESQQHSQGDTLQALVKNIKIEKQVAGRNKKNKKIQWNEKNTKIFKDLYQQYSGNLDQILLYFQQNNELNTKIDIKQIKNKFKIEKKKKSLTLLTQSQRISKNLNYQLKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDK6</accession>
    <name>A0A8S1MDK6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0550083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDK6"/>
    <dbReference type="OMA" id="GAFTYET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="307"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="413"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8075803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="472" mass="55800" checksum="0E3BA5D8543B257B" modified="2022-10-12" version="1">MFSNQSKNTIIHLIKDTVKGEKELENLKQSLILQITDSNISYQTCFLHLAYEKDRITRNDLEVFLQNQQIRFDQREISILKYTTYQDFLDLMLPNNQIFLKQYQQKQMNYNNPTSDILNLIGKIIGKNIEIIRKIQAYLDALMLRYDKNTLLNFLCPKTDKNMPVFLQLLKNIDLDISFEDVVCAMRRLDKDQDSDVNRFDWEELVKCSKPPKSNQSSRGNLQKYISKQQSQLSMHRSESRQKTDQENINSNRNVQLKSSTKSLQKHSSLMEEQILEFQLHLITIGKLNEELDQIKLELSSRADYSIQQAFQFFDLDVDGSIGLQDLKLGFLQLGIDVNEFIIRQLLHNYSTHHSQTQWDLNDFKCLFPDSKQSLNSYIGAFTYETKKIIKILIQKYLELIRYKKESTNQMGSDMKNIDIIFNFIDLDQDGIITQDDFKVLDVKSKHVLLFFQSFQEYPYQITYNQFQKYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ME27</accession>
    <name>A0A8S1ME27_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0550074</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Era GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ME27"/>
    <dbReference type="OMA" id="YVIDHRL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04163">
        <property type="entry name" value="Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22534">
        <property type="entry name" value="KH-II_Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:005848">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030388">
        <property type="entry name" value="G_ERA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005662">
        <property type="entry name" value="GTPase_Era-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42698">
        <property type="entry name" value="GTPASE ERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42698:SF1">
        <property type="entry name" value="GTPASE ERA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="2">
        <location>
            <begin position="107"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8075785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="449" mass="52360" checksum="391A7DBC2E26341C" modified="2022-10-12" version="1">MIKICGYCMSNLQKLKKYAPPKKLDAKTLRPYQHPTNPLSKKIDILSPTKPERFLFEDYETPATLYTINEQAIKTTELGTKVSNKILLKPLAQKPSVAIEEQKFLAISIIGPPNSGKSTFLNQMIGEPISAVSNKSNTTVSEIRGVHTDVKSGVQIELVDTPGVTKRYKFSKHFVTKAWDAIEDTNMVIILIDAIKTLDISMKNVMSRLNKIKVDQEQLRSYFRNEDDFRVSEEKPIPKILVFNKMDLCFNKKKLKWLQTEMEDLGKYDQTFYISSITGYGMEELKQYLYSQSFQCRWKYNENQKHLFTPMDQLEQIVRQAIFKRFYNEIPFIIGIYVKEFRVTSKESAKIEIQINVQTSSQVRIVIGEEGRAIKQVKNEIEIVMAEIYKRLFQVKLQVTVKQGDKQIEFDAEKEDRIEHAQLDETLYRKEERERKQLLNVKFSKFLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEB3</accession>
    <name>A0A8S1MEB3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0550215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8076063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEB3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="355"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="441" mass="51849" checksum="F26E10ED41DCC6D0" modified="2022-10-12" version="1">MEVIESLNKQALAQTDSHLALEILDRALGLLQYNADCGKIIDRNLIVTIIYNLAGVNQALCNLDKAAQYLEGTVFNLQEMIQQENNQVFLIKRMHLLAVVQLQYCGILSQLNQHQVASNVAHSTVVLINRLFEISYQYQSSQQTNNKKVQKYQKGQYIFEEYDFKNLLLKRSEELLKFIKQFITTQLIESEEQPKLQELANHIFTWKLNSLNNDKLPKQSFTKLYQRSMIGLLNENEWLLSFNVSNIMYIRLLTYEEVCLKGEILYEFSQKVLIEKLILLSMAYFSLASETRYLKNELKSQFYHLKSIYIVSSILNESSPYLDHLIQSFCIHYQQQLETILEEQSFVDQSTKVSLKRKESLMANKENTHDHLKQLIESLSKDKKFKQESIQKTLNQLQNLKDKLYKQRSETILKRFKTEPSLHFDKSDRNCKQILQQLMKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEE9</accession>
    <name>A0A8S1MEE9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dynamin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0510188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="AYNIEME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF233">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_59, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="1">
        <location>
            <begin position="27"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="182" mass="20922" checksum="66A34AAE115C2925" modified="2022-10-12" version="1">MKSGLWDNMRKMVTLIDDLRDCGVQDYIKLPRICVVGMQSAGKSSLLENICGLDFLPRGDGVVTRRPLELRLVHIQERLSPWAIFKEVPEKKFTDFTKVREEIDRLTDAKAGQRKSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKYYSECHYFRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEQ8</accession>
    <name>A0A8S1MEQ8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclin-dependent kinase 2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cell division control protein 2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">cdc2-related kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0610127</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="6">May form a complex composed of at least the catalytic subunit CRK2 and a cyclin.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEQ8"/>
    <dbReference type="OMA" id="CQGVEYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001908">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000057">
        <property type="entry name" value="Cyclin N-terminal domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF107">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 11A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="91"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8079110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="667" mass="79629" checksum="906CB84602089CAD" modified="2022-10-12" version="1">MLLKKKREFENQKGKENIQIQTSSNKKGEFQVKQKLLLPKNLLYKLFLYLTPKEINKLIRTLNHQYLQLISQQFWNYYNRYNPLLFDDLYSKFKFLQERRSKGKLYQVCNRLTGQQFVLRELDTIKANANHNDGVQTSVLREISYMKSLKSHPNIASIVQVKITNHIVSILYEYYPLNLRDQLKQTELTIEQIKSLFLQICQGVEYLHQNHILHRNLKPDNILINNKIVKISDFGMSRLETFPIIPYTPEDPKERERSNREIKRLWYRAPEMLFRKQYYSCEIDSWSLGCILAEMALRSPLFSGKTEIEYLFEVFQFLGSPDENDWNSISSDVKIKFPQWRKYSLQINELQKNDIVDIIQNRKQIYEKLITLNKVLGNDGIDLLQKLLCIKPEKRLDIIDIVQHPFLNGVIQNTQIHYIEPKFKEYLEFQTEINQTMRSTLIDWLIDVCIHFELMDETLHLSIIYIDLVLSIMKINRKDLQLIGVTSLKLADVYNERSKEYFKQENCNAYAYITADEYNEKQIINMEQQILSILDYNLTYRTPIQFLKIINSKLKVSNESQMLSYFFCDFFIMGYEQLIIERQLYAICCLLLALNTKNEQINSNQQFQFTEKDVIEAFDRFYNVWQFIKLQPQINSLEACMQRHKHLNPRDIQLNIPHQEFITQFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MER9</accession>
    <name>A0A8S1MER9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0590114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MER9"/>
    <dbReference type="OMA" id="PYHINTD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053035">
        <property type="entry name" value="Mitochondrial_GEF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46337">
        <property type="entry name" value="RCC1-LIKE G EXCHANGING FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46337:SF1">
        <property type="entry name" value="RCC1-LIKE G EXCHANGING FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="435"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="50"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8077999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="711" mass="83103" checksum="858E7FE91DA149FE" modified="2022-10-12" version="1">MNQMQNTNHLNPELFSAPKGIKIQIIDPIIKMEDELKNIMLSINEATFREQDTNQLLLQQQSLLQNLEEKYQQLQICMREVMQKFMSYENKNFIRLNENYYGNNQRIKNIMRELSQMFSVHFLEIGVSQRDIVICKQRRLNDRYDIQTQQPRRQLHEQQNQDEQQVITQPQIIEKLFEQIKQPLPPDIIREIFIQNDPQDQYCINISFNKPEEFGCEITLYRIYQLDIYDQSLSLQPAKRIVIEFENNKEQVQQQKLTFTRNHPLNKGELPFNEMIYLSIEAQNKNGWSSELELYPIKLYVKYFEPLSLFLQGTINSILINDTIQFLELFQIPQIIDEEDKSYLLIEDYSLVLLERQQIRNVSIKKDTVAMVNTRFQACQWGSILNFYDETITQHFDVDPIKDFDISPPYHINTDRAITKIACGLYHSLALTVDGNILSWGFNKHGQLGNGMTISSIQPQQISYFKNNQIFVVDISAGEEISICRSDLGDVYTWGKMQNMFGDNIIKVLNAQKEAINLQCVEQSNSQLVPRKINLKAKQIQAGYSCFGALSLENNFYMWGSNEHEVFGFLKCPELDDQIIVHLLDPIRINIENEYEIKDFQLGAYHCVIRVICKSNQSEQFLSWGYNKYYQCGQSASNKNAVKSCKKNQIDLLPQKKFKSYSCGFDRSVFASYDNHIYTLSKNDTKNNDCSVFVEEVMAGDLITLVLGREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MFR1</accession>
    <name>A0A8S1MFR1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Catalase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0610231</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the catalase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="PCEATRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018028">
        <property type="entry name" value="Catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024708">
        <property type="entry name" value="Catalase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465:SF9">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00199">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01060">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00438">
        <property type="entry name" value="CATALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catalase core" evidence="5">
        <location>
            <begin position="10"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8079267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="236" mass="26844" checksum="8D8A15E9E4B530B6" modified="2022-10-12" version="1">MSDNSDNMLTQSTGCPVDDNQNSLTAGEYGPILLQDTHLIDKLAHFDRERIPERVVHAKGAGAYGYFEVTGDVTKYTKAKFLDTVGKRTPIFTRLSTVGGEKGSADSERDPRGFAVKFYTEEGNYDMTGNNTPVFFIRDPNKFPDFIHTQKRNPQTNLKDANMFWDFLSLVPESAHQVTILFSNRGTPDGFRHMNGYTSHXDEYQKILYDYSLLQLQINFDYFIQFFSYEGISVGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MMC3</accession>
    <name>A0A8S1MMC3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0620087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MMC3"/>
    <dbReference type="OMA" id="MGCCQNL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="110" mass="12290" checksum="079261255BF3235B" modified="2022-10-12" version="1">MGCCQNLPLKETETIVMPQLSIKQEFSPKTLQMEPFSLNLIKGDIDCIERLGSTCADNILGEKQQSGVERQQSVQIKGEVIIDLRQAPSPSLLNKDRSSRNLNNKFIGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSN2</accession>
    <name>A0A8S1MSN2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0670191</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSN2"/>
    <dbReference type="OMA" id="NCAISET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040626">
        <property type="entry name" value="Pepdidase_M14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF11">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18027">
        <property type="entry name" value="Pepdidase_M14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M14" evidence="5">
        <location>
            <begin position="286"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="575"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="618"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="634"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8082312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="785" mass="91212" checksum="D2CC792FB2B8B277" modified="2022-10-12" version="1">MNSNNNNNNDSSSEDDIQVYETLTKLPPANELLPRKLTIDIDVKSLYPLKSLPQFFKTYVPFLEESTQRNCAISETPINDDNRTLLDYYDENKQNLVYKRFDQLDLGKRTSNDRTYFATLIANFKRAQPRVYFRGEALFDQKLTSCTSDQIQFDSNFESGNLFCAFKKSENVYDLVIQNDINTRGNTQWFFFSVTGAKAGQTIQFNLLNHLKSGSLFNEGLQPAVYSIKDNQLNGTEWCRDGFNISYFKSPFMKEYPLSLKKKYYQLRFHYTFKHNDDKVYFAHSYPYTYTNLLEFLNTQLDDQERNQHLSRKVLCTTLGGNTCEVLTITQNTLQRRAYRKGVVFLARQHPGEPQGSYVMQGIIEFLTSNNPQAEYLRQNCIFKIFPMMNPDGVVNGNYRCGLEGSDLNRRWKKPNKYLHPSVYYAKKYVKGFSKERQIILVIDLHGHSRKQSSFVYGCAYSSQVKTIERVFALLMAKLNPFMDYPSCTFRIENSKDKTARIQLWRELKINWVYTYECSFYGQQKKHFQVKDYLNCGVSICNALSQIVKDTSKEFTNQANVPDIQQQILEELGKMPQTDDQDNGSDSSQSEAELSDDELVQLFQPKTYTTKQKNYLKQQEFNNKKKPQPVNPKSTLNSQKASILQESPSKNSDQKPPMSSQKPKNTLYRIQAPQRLRQIEQAKQPDPPLMVDKSVQTDDWMYRQWLIQMGKMKKQQEFLSVTPTRNIYKSSSQSVTRFIVKNSSTKNDRGRVDSISLVSSGLQNKLISSSQQRQMMRYSGSNFMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTH0</accession>
    <name>A0A8S1MTH0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0680100</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTH0"/>
    <dbReference type="OMA" id="QLDECCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="494"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8082682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="892" mass="105219" checksum="950F24C85D63E56C" modified="2022-10-12" version="1">MTDDIEQTLYNEFKIQGSSRMSDTFRPQLIQMDMQDIPFKENSDYDSIPQMDSLQNIRFRNISNSNNQGKEVITSKTFPHQNDKIKLYKSTGMLKMLIVNNHVNSFIEKLKKKAYIFPKNKSKQITEHLPEFFAKIKKNWRKSKQIQQQDENSINSISKRLPVFNPASKLMIFWEFCRAIQLTILLWWLPYKIAFNPSSNSSIEAFESSLTYLFGADLAIKFNRGIFDQGKLIKKRIHIIKSYIKNELYEDVIYFITLIFVISDIGIKTYSFQEVIVLVQFGLNFIKLKKYLNKYEETFVESSYLTEVLNLIQLIIITFYFAHFMACVWHYVGVKSVESNQISWTQDSQFDNSNTLQMYVFSFYWATTTMVTVGYGDISGKNIYEVLCAIILMIFSSGIFAFSMNQIGSIFTNMDAQKQQYKRTLLLINQYMNNNQVEEQLQGRIRNYLKYHFHKQEKLYKNEISGIIDKLPSNLKQELIQDVQFRVMQCIPFFNKNFSEEILPQIACELNLQSYTPREIIYQQNQLDECCIYIVWKGEVNLIDDNSGKILKRFTTGQCFGELEFLTNKRRQGTAISCDFSQIYSISRMQFLKILNSYNNDFQQFHQLKDSILFQYQSSPLQCYCCQESHCIWRCPYIHYKPDLERVIKKEFVKETYQDRSYFRRKGDRLKVFRQDSIYQSTRHIRTSNEIEITQPIASGIQQVGSISESNESGEESIKYEKKSDQVLTLSYCYPNDNKRKTVKLRPLALSPVQPAESPKARPAEILRYGQKQRKSYFFPDNSMINNNVVNKRRESVKRNSSIIQTQNYNKQSSGYEVDINQEPFQIMDGIDKLIIYGNYFSEGNYNKIIEGYLRCQKKCKLLRKHMIPSKYAFVRLQKFERLKTHGKKINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWY8</accession>
    <name>A0A8S1MWY8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0710181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWY8"/>
    <dbReference type="OMA" id="HKVDFQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8084109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="215" mass="25360" checksum="DB83F61D7D213D4B" modified="2022-10-12" version="1">MGSFCPSSNIKVIPSPVIQEEIIDPQVEIDTLINQETNYKLSLIHNFSVENPSLQTCDEVILEILSISISFMVKDFSEQSNFLKIQIQKQVQDLIDHKVDFQEDLLKMLINYYDFLSYVQENGKAKVISWWKDPVGVVEIEQALKHWTQEFIKYGGKLRREKAFRKLISDPKPPERFVKTREWIKKQLEGNEEELKPKREKVETYNNFEEGFEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXA7</accession>
    <name>A0A8S1MXA7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">60S ribosomal export protein NMD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0670087</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as an adapter for the XPO1/CRM1-mediated export of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NMD3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXA7"/>
    <dbReference type="OMA" id="YHNNTWR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048899">
        <property type="entry name" value="NMD_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048898">
        <property type="entry name" value="OB_NMD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21193">
        <property type="entry name" value="NMD_SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21192">
        <property type="entry name" value="OB_NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="9">
        <location>
            <begin position="21"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 SH3" evidence="11">
        <location>
            <begin position="252"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 OB-fold" evidence="10">
        <location>
            <begin position="320"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="485"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8082106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="555" mass="65160" checksum="8BADE6067D77C16F" modified="2022-10-12" version="1">MMETENQQIGNQIKPKTTILCSICDLAFTPSEQKTLICTNCIINQTDITIGITKEGIVNYCRFCHRYLRPPWTLCERESKELLSICLKRLRGLNKVKIIDAAFVYTEPSSKRIKVKLTIQKEVLNNTNMQQTFICEFLEHYQQCEDCKKEFTPHTWGAAVQIRQRVDHKKTFFYLEQLLLKYNANDKVLKIEQVDDGLDFFYKSRSHANRLVEYLSTILPIRVKQSKQLVSHDASSNLFNYKYVYAVDLPKVCKDDLVILPQKLCKELGGVGRVQLCYKLTTIVHLIDPLSMKCSDLSIEAFSLYENEIEVYNLKTHQHEFTVVDVERIHTRNLNESYISSSQNTFLPKLTKVTIIRESNNYVPITVKSHLGDILKTGQVAIGYDLTQLTIESLEDISNYPECILVKRQINKEARKLRIFKLKRLEAQNVMEEEGKEKKRNQQKKQNDQDEQFEEFLDDVEKDKEMRKNINLYRDEEKIKKLSKEDLKKKQMKQVQQQKKDDDDWQDEDEDDIKLEDLMKDLNLEDKPELAEPVDEAQEEINQFITKLEKVTIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXY1</accession>
    <name>A0A8S1MXY1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0740045</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXY1"/>
    <dbReference type="OMA" id="CTALQFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09599">
        <property type="entry name" value="M1_LTA4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.30:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049980">
        <property type="entry name" value="LTA4H_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034015">
        <property type="entry name" value="M1_LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015211">
        <property type="entry name" value="Peptidase_M1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726:SF3">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09127">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01263">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal" evidence="9">
        <location>
            <begin position="496"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR634015-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634015-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8085258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="644" mass="74343" checksum="ADBD04219C5A005C" modified="2022-10-12" version="1">MIYLFLCIALTFGAEVDVNTFSNYIEVSQEHVHIEWLLDLDGQYINATSQSIFRVNSNKLKQIDLDIYQLNIIAVYMTSTGNVLKHNIINKGDQSLLQGDVLSIQLDRVYTRGEYINLNIKYNLDSKGARALGFLTKEQTYSKTVPYLFSQCEDNNCRSIIPLQDTPSIKIYFTATVLVKDPRINVFMTGNKLYNMKFKLLNNYENLQALTIYQFELNIKIPAYLIGIIAGEVVEKSTGNGTYVIAEPHFIDDYAQELSDLPMYMKEMQEYIGPYIWGDYKIVILPASFPFGGMEHPLLTFASPTIIVGDKSGVGTAIHEIAHSWVGNTVTGRNWANFWINEGFCVFLERKILSKLNGLDSVKLDAINGNSSAYTSMQTFGLDNSFSSMHPNTTNRNPDEATSRVPYEKGYQLLTYLETLIQEDPFQKFLRDYIENFKFQSIDEDQLYQFLLNWVRKNKQEDSQKIITEIQKVWKKWVYEPGLAPITIDVTTPLFTNANNLAKAWIDGKGEIPEQANDFLQYKPNQKSVFLQYLIDNYKDVETAVMTKIDEQYKLTFYKDQKTGYKWYRLILLVKYDQGLEGVHDFVSKVGVSSYLKVLYDLLGQNYNKQAYDWFDENKTFYHPVVVQAIEKILQKYPKKLIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N110</accession>
    <name>A0A8S1N110_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0690107</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Lipid-anchor</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N110"/>
    <dbReference type="OMA" id="KNGFIMF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001799">
        <property type="entry name" value="Rac2 family GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8083093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="191" mass="21809" checksum="E7E0B2694B740C54" modified="2022-10-12" version="1">MQEGKKELKLIIVGDGAIGKTCALYSYVNDKFPTEYVPTVFDNTVTQVQINGEFYDLSLWDTAGQEGYEHLRQVCYQGTDIFLVCFSLVSDVSFENAIKKWLPEITQYNKNGFIMFLGLKKDLRDPKNPNHIQTQVAETTIKKLGYQLRETSAMTQEGLKEAFSFSIQSLITQRQEQVKTKKPKKSSCQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1W3</accession>
    <name>A0A8S1N1W3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0700202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1W3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="290" mass="34793" checksum="70EDC48D794F682C" modified="2022-10-12" version="1">MNIFSLLHQYLDCQLQQVQVEYYLQSLFITSVIDFEMFDMQTRMFNLRILQLYAINHLILQQNIKQKKIVQLILLWDRIYYFQQIKNIKPKSFLWSQFNRRLLKFQNVTFINNILHVCVQTIFKYPHNYLTPIVFSASLIQPTPIFLNSLYNSKNNIVFIKNQQSAILISSFYQNCQFNYQNIQPLLLWWFLESEYLSYQQIKSIFEVKPNAEVGLFQVENLEIMFCNIEQSIGFQGRALYIEAQRNSIILISQFQFKNISTLFSKYLGYVDLSIQMELKHKLLSYHSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N3B6</accession>
    <name>A0A8S1N3B6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0760173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N3B6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="26303" checksum="205D2608A349DDB0" modified="2022-10-12" version="1">MGICRSEPLPGGFQQQEGKPEDVKSGLEENPQQNQNQQQNENLQKNENNENEAAVKIQSSIRGKKAKQELQKKKEELESDKPKDWIEYQQQFIEPPLPTQFKDLVNYKPTNENRILPPYLGPDGSVYNGQWNKGEANGFGQMLKPDGTYLKGLWKYNIFSEGGILYPNGDFFIGNSKYGQRFFANGVIYKGEADYGIPHGQGEETHPNGNKNPAIYRYGQKVEQEISQQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5K0</accession>
    <name>A0A8S1N5K0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein arginine methyltransferase NDUFAF7</fullName>
            <ecNumber evidence="7">2.1.1.320</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0670187</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NDUFAF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.320" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5K0"/>
    <dbReference type="OMA" id="LPFAPNM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035243">
        <property type="term" value="F:protein-arginine omega-N symmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12710:FF:000012">
        <property type="entry name" value="Protein arginine methyltransferase NDUFAF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049:SF5">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8082304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="390" mass="46045" checksum="460FFED0E6B13B02" modified="2022-10-12" version="1">MKRMLVRDFIYDRLYHPVEGYFVKNIQLGALKNPIEFKQLLGYEDYTKKLAENYPENQWLTPSEVFRPYYGITLGNYINQQFRFTRKEKLRIVEIGAGYGAACEGVLYYMRNHQPQTFSNLEYHLVDISPEACVQAEKRLSQDFKQQIKKGNLRIFNQDFLNYKQHTQNNEMWFFVFLEVFDNLAHDKVIDGKQVYVENMKEFAETITDPLINEVYTMYQQFKQQNKNQDENIEDRFLFNTLRKLISKYYGNQKSNSIFLPTGALQVLKHIKSNFHNPSLVIADFDLLKNNFTQESINAPIVSKKLTKPHERLDYESYLVERGAADIFFPTDFNFVQYMVKQILGLDSQIFKAYQFADQFSQSSWTTTKSGYNPLKEDFGNTSFLVTDHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5Q5</accession>
    <name>A0A8S1N5Q5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0760226</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5Q5"/>
    <dbReference type="OMA" id="DCIRAQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8086469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="51" mass="6162" checksum="1E52034291E40148" modified="2022-10-12" version="1">MISETQFRRDLNMKFSQTSVIHDLSEYGIQQGSSQQDFYMKLFDCIRRLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCZ2</accession>
    <name>A0A8S1NCZ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0870037</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8090927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCZ2"/>
    <dbReference type="OMA" id="PFNEKEG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="13"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8090927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="539" mass="64329" checksum="ABF6633F737724D5" modified="2022-10-12" version="1">MEEEDSKVNDWIIDKHVLLGKGTFGKVLGCHRQDNENELFCIKIISKFNIALRDDEINKSISNEKNVIQILAQAKEQSENIVKIIELIDKPQYIYIIMELCDHDLLKEFELIKPNWFTIQEQIDIIQQIIKGACFLKDNQLIHRDIKPQNILVKIVTDQDQNKRKIFKIADFGLSRTLENMYQKTNLTLVGSKNYYAPEIYYQIQFSAKCDIYSYGLLFYQIAFQGKLPFDENEGQKEHFDKIKNSNFKCNKIQEKYGDLITDLIEKMVVFSEDNRIGFEELQKHNITKLKLTMDRDSIFFPFCDINTEKDIQAKETRNQKQISENIYKIINIYYRKFLLCQHIRDHLKSKFQPLDQYLKILLILIGWIGFIQIKHAFSLIKQISTDFEGILAQSIQLPLLLQYLKTYREQVQKTEQYQKINQTIQQTYYQFNIILKNEFNALLMQIYQDKNKMILQKYQKNLEQKIQLIKRLETQNINCNILYNELNDLMNIEIYQNKQIFDQKDLDLIKRVENLQKMFQIVDYTIIDVNQIFDPNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NE80</accession>
    <name>A0A8S1NE80_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0730107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8085024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NE80"/>
    <dbReference type="OMA" id="RKSHTTH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001034">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="109"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="487" mass="56439" checksum="8F4108CC5D60CDEB" modified="2022-10-12" version="1">MQDDRVLERSPQDTLRIPPRLSMMDRKCSGRFTFVKTQAEEQKEAVQNNMNRIKQQFRCSLNQIINKPIQPLQIREEQLFELGRKLDIEITPSDVENIVPNTKEFFEEYEIHERLGEGCLGLVKRVVQKRTGQEFAVKIVATQDDEIIRNMINEFKRLIKLSHGNIVKAYKMFLDFDNGFQSESQAYVILELIKGKEMFEVINEAGHYSEHDAKELFKQLLSAIEYMHRNGICHRDLKPNNILCLSDKKLIKVTDFNVSKFSDSYKEFGNLQDTEKIEMWTYTGTVAFSAPEIFTGEGYNQMVDMWSAGCILYSMLSGQLPFNADYLNDLIESIKLAKYEFPEEIFQEVSSEAKDLIQQLLQKDPALRPYPDEALNHPWFSETIVRKSLRHLTINKNISHLSSRNSNKLKQRTILLNRGHYNGSSDGDEGYTIQDMKIKGTARKSLFNTAIAPLEQQSDNKCLKVQNGVLRKSHTTHIQEFTQNDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NED2</accession>
    <name>A0A8S1NED2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0820024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NED2"/>
    <dbReference type="OMA" id="NEEETEX"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033192">
        <property type="term" value="F:calmodulin-dependent protein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097720">
        <property type="term" value="P:calcineurin-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043360">
        <property type="entry name" value="PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45673">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="2">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="357" mass="41583" checksum="89B73F28625C4EDC" modified="2022-10-12" version="1">MKIYYPTRVFLLRGNHECKQLTSYFNFRDECLYKYDQEVYEAIMASFDNMPLVALINNKFLCLHGGISPDLKSLADVERIDRFREPPKAGLFCDILWADPVENEDLYDEIHYRGNDTRGCSWFFGEKAVQPFLDNNKFICLIRAHEAQLEGYRLYKYNEKIKEFPQVLTIFSAPNYCDVYNNKAAILKFENCTLNISQFGYSQHPYLLPHFLDIFSWSIPFVTEKVTEMLNYVLQPRPGEKIEDSEDEIPIPKVITGQQNMGQSQSNSRPSGKLQQITKKQIDITKSKISFVSKMMKMQTVLRQERENIIKLKGLCPDKRIPRGVLLQGAEGIKDALEHFNTTRAADRINEKWPETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NI35</accession>
    <name>A0A8S1NI35_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetraspanin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0890053</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NI35"/>
    <dbReference type="OMA" id="MVIFTFC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8091772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="31643" checksum="67B23FA0F603791A" modified="2022-10-12" version="1">MCLPVSCLKCIVEFEAILILIGGLGSFGFTIYLQIEYNIEKQDDTNQLGPGTIVTFWIISSCMILNGIIGILGGKKKSACLLLLFNLVNIGLLLGFIALMILGYVLANRIEKNDIKDYCSSTTPLQQTQNLGQNLLCSSDCECYIENPGNLPPSYVNYSTTDTSQATKVQDCQYYSDNWNDTQKNQAYFFLNIEKKYDCSGWCNPFSLQIFYDINNNFDQSNDQCLFSAQQQLYDTCQIIGDISAGMTGLMGLMVIFTFCLCCHPTKKDQDYYQKLAYYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJZ4</accession>
    <name>A0A8S1NJZ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CAAX prenyl protease 2/Lysostaphin resistance protein A-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0860209</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJZ4"/>
    <dbReference type="OMA" id="RVYFINE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="2">
        <location>
            <begin position="125"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8090786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="233" mass="27521" checksum="422D77B66F907C59" modified="2022-10-12" version="1">MLGGLYGLNFKQHTYLFLCLILPANITGIGLFYLDEALTTLCVLQLFYVMIPGIYIKYLSKEGELRVYFINELEGRQEQIKRGFSLFRSAFGMVLLSLIFTYYWEEEIMDYLKIPLLEHPIYIGAFMFVLIICNPFLEEWYWRLFLMKTYKESEKYRLLINLFYALFHFIILFKIFKSDLEFAIGFSTYFLSIGGSFEHIREKYGFITCLMAHYGMTLAASLALLVVYKSQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NLV8</accession>
    <name>A0A8S1NLV8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0920138</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Assembles a suppression complex (suppresome) by tethering SIRT1 and MDM2 to regulate composite modifications of p53/TP53. Confers both deacetylation-mediated functional inactivation, by SIRT1, and ubiquitination-dependent degradation, by MDM2, of p53/TP53, promoting a proliferative and cell survival behaviors. May play a role in the regulation of spermatogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Acrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NLV8"/>
    <dbReference type="OMA" id="HEVNSQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052472">
        <property type="entry name" value="MORN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511:SF1">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8093132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="23332" checksum="4E0F8F2380F1EA07" modified="2022-10-12" version="1">MQSTNISSPQKPSTASPSKSIGPYRTITTTYAPFTILQKNSKFEDSPIKRVKQFDDDKLIKSIKLIKPNREQSKSCSKSQDKIIIKDIKDDSQYIGSYNGKVREGFGKLYNAQGILIYEGQWYNDQYHGNGILFNHEVNSQFTKYLGQFQFGYKYGAGIEYYRDGSMYVGNFESDCRNGMGQLTKKNGEKFNGLWLKGKMISRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUP0</accession>
    <name>A0A8S1NUP0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0980002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NUP0"/>
    <dbReference type="OMA" id="GYEYEEP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="302"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="622" mass="74744" checksum="0B676078834BBB02" modified="2022-10-12" version="1">MNKQKLAQEKQYRDQAKYRTLFSQEPQNPFGEKDVEKMKQQYAQILKEQVKNTQRHKEQQKTIDRQETQKQIQYESPFGKDRLIHLQKLNRELLKPIQDIDEFVKKKNQTDISRPKTILKNQSTERSNQQYSNDNSVEQVPQYNFDSPNQQENKEYFQNRNQNQILPTQQTQRIRDYQSSQSLGLPEPILQNNSNLLMKNSKNINFPIQLPPKTPGIVYDKQNNPILSDYVSIYDQQFSRLHKSEKSDFMKELNQLRSQFFVQQKPQVQPFKQMANLQQSPPKFTQDVGIQDDQYRQELMERERQEEQIRQQEYLRQLEEQKKREQEEYEKQLREKIERELEEQKRLKQQEIEIQTEKLVTDTGIQHVDQNAVTAAEYMKTLKSLSGSVIDMKNQFIQGQQKLNYELIQLRDKAQRDIEEKLRIQKELKELEQRNQAMKVWEGEKQRQLMQALEKNAPIPQYKSAYMDPKQINPTVKKVIRFKDQTQGYEYEEPNGVMSKSNLMPLNGGYDQKHLHNLKANEPVLPEELEVFRQEIAPQLKYSNPITRQVRMDDLNDQFYDYKYQRQLEHPGQDRIERSKYETQIKNDPNQVLLENDRRLHELRIIDPRGDNKQIQELYDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWA4</accession>
    <name>A0A8S1NWA4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0940069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWA4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8093813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="193" mass="22856" checksum="B6908029BB623530" modified="2022-10-12" version="1">MQVISALAQITQEEDSTSASEKLGQFLQTLRNKVEEAWAQYTAENTNALGLFSQQKDLWFIIAKLKTYKEQFQLQPLSPKLLNQNQEFYQKIRIVKLKIQQKQEKLRHFSFSNKRSRNQNLFEDANDLYHSFDVEYEIVTTGRRQELVLVNKLKDWLLEELLNNNNDVKQINIFSHKLNKLIISSFDLEFINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXA9</accession>
    <name>A0A8S1NXA9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0970129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NXA9"/>
    <dbReference type="OMA" id="KRFMEQK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="162" mass="19567" checksum="3A5387AEA1343FB8" modified="2022-10-12" version="1">MKQQIIQPRAKSIHIKEAYSSRLNNFKSLPKLIENNKTSRYSEHLSPSPKPKFYNHWYIPYEKRFMEQKELKNFHDEIRSNYAQMKEDPLFLYHNLNRPLSIHQDPFSKPDKNQIQKIKKYDKTKIFLEMNKSSGVLKLFLEDLVQKKQRVPAFLMNEQLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYD3</accession>
    <name>A0A8S1NYD3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 299</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0970020</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in spermatogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYD3"/>
    <dbReference type="OMA" id="FNNYQEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027887">
        <property type="entry name" value="DUF4464"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33588">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33588:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14713">
        <property type="entry name" value="DUF4464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8094763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="240" mass="28143" checksum="EECF950A6749B93F" modified="2022-10-12" version="1">MMIAEDFDSEVYDTSLDKFKTYEEYLDSHITKEDLFYLEDIELARQLKEQGYHAKTEILSREQFEAKKKAVEEARLNQNKDKTKALAHTEVSDPSIIEKSPFLRALAERELKVLNGKLLTIIFIRLESTNCEVSGYIDYAHRLKTEDFKIYFEGKKKLQPRPTDLSYYNWRTGQCVSNDSPNFKVDANSGQQGLLFRNKRDRKVINVDPNKKPEDGMTRTEIESPEAIQIVLYDYYTRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZY3</accession>
    <name>A0A8S1NZY3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1010119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZY3"/>
    <dbReference type="OMA" id="YICVDPI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="262" mass="31046" checksum="A56A956F497BAB6F" modified="2022-10-12" version="1">MDLMNCQNSNHDSQRIKYICVDPICQVQQKIGCADCFLEDHVTHQRRTVEQFNDQVKQQLDSFNQIEFQPITNTVQLDLEKQIDKELENCLSCIQYRFTSIKSDLKNLLDGENQKLQESYNVLQQSKNHELDSLKNVNDLHSLNQEELNDLVKFYQEASKIQKNYIKASEIFYDEKQKIKQKKQKYLCKLQQIMQGLMKDFNELMVTKNLQFDDFSEFETPQKSSMSPNKMTSFVESTRSSRRFYMNQSKKLLFGNAIRKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P146</accession>
    <name>A0A8S1P146_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1030158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P146"/>
    <dbReference type="OMA" id="TMIHPVK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="254" mass="29334" checksum="31ADD86626D64DD8" modified="2022-10-12" version="1">MLSETSKEVQKDQNVVPQLNQTPSSTYFVYNCYIPVVQDYMSQPPPLQVRNSSESFPQLIKNNDSIQLSHLAPGVSIPSFQFGGPSAPFVSPDDVMRNVQPTQNHFASFILNEQLKTQNQPTKNRIIEGTQTLCDIEIVLISRYFDMKAHVFCPPNNKYTLKEDTNISNAEWLKDRMLFRYKKPIKESFSDMIDQLEKEGEMNFKNMVHPVKFAQGGFDNIERQVPKSVKSEKPFSVFIFEIQQLISQFLFTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P547</accession>
    <name>A0A8S1P547_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Malate dehydrogenase</fullName>
            <ecNumber evidence="2 6">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1060076</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="2 6"/>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P547"/>
    <dbReference type="OMA" id="GMIGSNM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000010">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000002">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010945">
        <property type="entry name" value="Malate_DH_type2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01759">
        <property type="entry name" value="MalateDH-SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003916">
        <property type="entry name" value="PRK05442.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23382">
        <property type="entry name" value="MALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="7">
        <location>
            <begin position="9"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="8">
        <location>
            <begin position="157"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8098153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="320" mass="35405" checksum="D7BC6811DB89BE9C" modified="2022-10-12" version="1">MEVQQSLTVCVTGAAGQIAYSFIPLLLSGQVFGQVKINLRLLDIPKMETALKGVVMEIEDCAYPLIGDIQTGSNPKELFKNCDLIVFLGGFPRLPGMERKDLLQKNANIFKEQGEALQEVGKEDVKCVVVANPANTNCLILSRYATKIPRQNFTCLTRLDQNRAYAQVALTLNKPLNSLKNIIIWGNHSTTQYPSIEHATVEGQSFVLPDQGQFIERIQKRGAEVLNARGNSSVFSAANAVKDHIKDWYNGREDTLLSLGVLSNGEYGVQSGLCFSYPIRCLGAFKYEIVGNLQLSDFSKQKLKVTEEELIQEQGLAESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7A2</accession>
    <name>A0A8S1P7A2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1080077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7A2"/>
    <dbReference type="OMA" id="QDEMHTV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="115"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="190" mass="22286" checksum="F66DF6390A629D41" modified="2022-10-12" version="1">MSKHRRTKSSGNYFQINHEFLVKDDDFEYNETKFIIQKIQQMATQTQQIVEKLDVNTQGMYNNLFNFGKQQSKEKVDLNLSQISECSSTHSAEYPSLKLRNQGIQSPILMHAQNSQSLQQTIQYLEQEIEQMKIQQDELHTVNMFLLQETQKNRQDLLRSQKENEERIKQLILKIDKEQSNANGTSDGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P918</accession>
    <name>A0A8S1P918_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1100005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P918"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="327" mass="39032" checksum="665E602B9FA24739" modified="2022-10-12" version="1">MYNTEDCSQPKFEDLKNLKCANPEHQNPIQLIILDQNLKQNQRLLCQSCIKDFESNQKTIGFERAISLTYENYQKKKELRDIVLNENIKLLEELKSNFLSIKTFVNQIIEEMMQSTQFWMKDLNQIKQQQYQFYDEIDQLINQNQNIEIDQIVQETKTLNNKWISKLKEKIEWFSKFQSYSDCVNKLKNLSEGKVENSQLKNDLGLKCQQQQQTLQSDCKRGNNLNQIGNLKEEKTNNQPQQQGKKNSLKNNQSIHYPQQPQYVPAYIPKKQVCQQSKYQYPGQLKYNNCNMNNKPSYNRREQQYLPKQCIPPSRQYQFDQDRLKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHV7</accession>
    <name>A0A8S1PHV7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1190090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHV7"/>
    <dbReference type="OMA" id="GFRMMEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8102807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="29531" checksum="D4365D0F1920258E" modified="2022-10-12" version="1">MIVVVASNSQYVSFATIVRLMLIIIIYCKCVWIKNKSIKRGIIISLFKIICSIGFESYTAVKIDNSMNFYTIYQAAIMAVLIVNSILLLLLMYFGWRIYVEINEKSFVQTSPDGFRMMEQLSAIQQQEQQEKSIKKEEIKNPTPDQPELDDKGSVNISINENMSPAMLKLKSSLSPSPGKYRSGKILDFLEKKISHAKNSQKELSNGFSREFNRKFNTDQIQQISLDFGDQEDISSARNPIDVQIQNHNFSKDIKFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIX6</accession>
    <name>A0A8S1PIX6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1190018</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1190020</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIX6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="58" mass="7131" checksum="A72D07DBEB71EE31" modified="2022-10-12" version="1">MNKKFSLIFQKYFETSHSYSDVTDLNRKGRLFKYLINFQMILKRIYKGQSRLRLLHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJY9</accession>
    <name>A0A8S1PJY9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1200152</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KFQRVHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035764623" description="Insulin-like growth factor binding protein, N-terminal" evidence="4">
        <location>
            <begin position="16"/>
            <end position="1743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8103311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1743" mass="199092" checksum="B9AE12C43A3BD3BC" modified="2022-10-12" version="1">MQIFVFIILLQISASQWLPGDSQLITNQIYQEATNDQAYLYTENFIKSGTQYTTANFINCQSPLTSYITLDQHTTKVENIIWLNYQKGQVLSFDLYFHGTWNNQPVTMKIGSFSETIIYTSPTIYQTSGFCDNTPYEVKTFNYQFMNSYSGKMSFESSNINGMVSIRNIFTTQIKCYPSCMTCNGPEHNQCQSCFYDTISNNVCPPCPINQYYVKYLGCRNLCDFDSKLFKNGFCQEYPGNIQYYQVNYFQSTQFQNNQFYFENLKWSIKYDKNHFDTTVCLQFNVGMLFYGIFKYNSGIQRFVNEINQADGSKSIGLKIMILIFGDIPLEGGISIKLNNTYYGSIFRSSSGIQTHNVQIFDVISLEPCLTYSNCYKYELHMFVDIPQYPFLFSSQSNFTDNSAGWGLISISVTAGYCPIFCKKCVVSFECEICDQQFLKYRDGRCIYIHSCVQPYQKKNETHCIDFDDETPYSIFLVKEYIDYANDPTQISKYSILTQSSSPNVNFLKGTSIFYSYWKSYRVFGGPYVWAQAKFQRVHQIENPHHGITIGFYILFGPNFPSSGSFVYTIDFNPSVRITISDVPNPNDDGTKTYKVYQKINHVAGSLKMIWDCQGVNNEPITSYCAVYNYYIAVHQCQPFCLQCINEFSCTIFDDVVEDPNLLKFSQSECQSNQYYNRNEVKCLNCPSSCISCTSPIDCQDCQTSYTLTKLGCVCKQNQYEDSNQCKDCPTLCAQCLSDSYCIECFLTDFREPKNGQCVCMPEYYSVASSSICLQCHVLCKVCNGPSFHDCQICKDIIGIEQVGTTCQCPNQQLYNQLSNSCTDVQLPFSDVCGDGFVTDQEECDDGNDIQFDGCYNCKFQCQESCTKCQLGDCQECAGGWYIDPQTWRCKERCGDLILVGQEQCEDRNQLDNDGCKECRFQCSIDCSSCNQLASICESCQFPGFIPNQYFCENNCGDGFVVVDPSGKFTEICDDIGCNTDCKSCKEGYVLINSICEPICYDSLLVEKEMCEDTFILPYRGCQNCKSKCQLSCLICDTTGKGCLSCEIGYEIFDYICYSICGDQIITIDEECDDGNLIIGDGCHFCQYSCQDSCLNCIKGKCLNCLDNYQLVISTCQQKCDNNIPNPFGQCDYIISSNDGCYQCLQCPENCDFCQNGICVICQNNFELDNIYNQCIQIQFTQFEIQQNCVQLRDNQCLTQSEAFIDLITNQDFVIDRCPQNCKICIFNNCMECQQGYYGKICMSKCGDNIIVSEEECDDGKGWLPNECYNCKLICPDFCKSCIFGICQECQSGFYIDMISNTCNTICGDNILASNEICDDGNNIEFDGCFQCQYQCEIECIDCQFGKCVQCQEKFILSKSKEKCESVQSCEPQDGLYYENNSNSCVNLCGDGIVAGNEECDDSNTIPYDGCNECKFQCNQQCILCEKGICLIESCQLGAILINNSCCGDTILNGDEECDDGNQIDFDGCSNCQFQCNKFCSVCLQGICVECIEEYKLEDNKCIKNEYNVTENQQSKKIDQNQDEICRNNECAFSLRPQMRLVFINQTFNHQYIEINFDQQVQLAALQDEEQLQIFEILIQDLNEQYYNIQLYTILGIGQDLQDVQYVIDIEIYLELQEKPNLQITLIQNVVNFNNQTIIQPQRSILLYIPRIMSQELKSKAISISKTNIAFMISAISISTVSLIFGEASLFIETLSTLQYQSYLKFINLEYPENLYMYFQTSEMYYYKKGRESNQNKWKVCNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKX1</accession>
    <name>A0A8S1PKX1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1220103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKX1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="154" mass="18903" checksum="FD70217332B21E85" modified="2022-10-12" version="1">MKSYCKQNNQKCKIRQGQYLRIQQRSKQKIKNHGFVTQHYCKALLFQSSILFERLTEDSFEQEILKFSIFQKNLQKHYLITSRIQNLKKNKCKRNSLIFHQIQVRDVKLYCLNENFRIIIYYQNETLIYYIENDYFRQIFNEENHSCVQNSLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLI8</accession>
    <name>A0A8S1PLI8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1220179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8104077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLI8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11763" checksum="B6CE14476AB02C4E" modified="2022-10-12" version="1">MVRAKVLESGEYQNDKRSGIWVYIYDNNKIGGEQYNEQGQKNGKWIKLSDINTQLTYIGEYKKGQKVGKWDTDFNYFGNKQIGGGLYDEEGEGIKIGLEDGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMP3</accession>
    <name>A0A8S1PMP3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1240061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMP3"/>
    <dbReference type="OMA" id="NGIIIQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000810">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF68">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATG1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="288" mass="33019" checksum="288E481203D4C298" modified="2022-10-12" version="1">MFTPEQFAANYQINFSKLIGKGHDSTVFLTMNKQTKQQFALKCLAQTNEQNAINLFNEIQILRHIQHPHIITMFGYCSDCSCMLLELMTNGSLYKILLQGPLPLAIANGIIIQIAQALEYLHAKGITHGDIKLDNLLISGDYIIKVCDFGFAKINGQTPIPKTTVSGSEGYTAPEIWQIPIDLKKCDMFSLGVVYFIIITGHPPFESNNPQMEDAWWKLIKKEEWNIFWKELKLTILPEYVRIIIQKLLCVNYQMRYTADEIIQILMDKCATSDQIVDEIKKRIIQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PN72</accession>
    <name>A0A8S1PN72_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1250075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PN72"/>
    <dbReference type="OMA" id="TEDEWME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="45"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="111" mass="12770" checksum="085A5830510D9544" modified="2022-10-12" version="1">MGPTQGKELSPQMRDRVLKLFARFDVDGSKSIEKSETIKYWKSNFAKLNTEELFKSVDTDNSGTISEEEWLNFWTSVLRSGHTEEEISDELESIESGSSWVKFENLDKKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRS4</accession>
    <name>A0A8S1PRS4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1270017</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRS4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01610">
        <property type="entry name" value="PAP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF28">
        <property type="entry name" value="DIHYDROSPHINGOSINE 1-PHOSPHATE PHOSPHATASE LCB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="353"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="79"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8105329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="410" mass="48069" checksum="4D47C0953CBB4362" modified="2022-10-12" version="1">MKYNSQKNAILGIILFSTYIIIDQFIDQILLQYTYKFIFSIKNPSFIEISYMIKSTSLLLSEIILLYFYLSFNKKLDVLLLILFSCVLTGTHAFLKEIYNQGRPYMFNSDVQTLECIAELGKPSGHAICIVSYMISIYYADHFENEEEMKLPLFKAILYTIIALIVCLSRVILGVHSIGQVIYGIIFTLCMYMIYLNIVHTKVKKFILKHIKYQSHRLTIFTAVLFLVFLTLSLSADFYSRNYFAQNPNLYYKFMDAVQECRERNGLEKLNDESGFILQRSTSQSYGYYFIIIGALLGLVIFRGVYEEGLYEVQSKFRTRLTLFIRIMLFYFMIFSVQYGMEYYEPEQYESYLIMIVIGHIILGLCITLIYPILMYFLGLEIYGSMQCVNKSTLFIDYSHIWESESMISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTW1</accession>
    <name>A0A8S1PTW1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1310016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTW1"/>
    <dbReference type="OMA" id="HEYDEVM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8106492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14960" checksum="8DB0090E661E71C9" modified="2022-10-12" version="1">MQTVWTLIHPTRISVQKTIKNLLPSGQEFFGTVIRDSRNKSCKVKVSYKYWHKKYKSYFGNSSEFIAHDEDNFCKVGDKVVIKHCQKTGGKYYYVRNVVIPVGRNPSDVQDDVMKELQNIRTQLVQSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PV09</accession>
    <name>A0A8S1PV09_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1300131</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YNGATVH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035931089" description="Insulin-like growth factor binding protein, N-terminal" evidence="5">
        <location>
            <begin position="18"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1087"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1133"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1187"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1228"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1250"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1281"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1312"/>
            <end position="1331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8106458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1367" mass="159363" checksum="78DB23481384F422" modified="2022-10-12" version="1">MFIWFFHFQFLGIIVEAYYLQHFVTSSISGDEGWLIKNPYNGATVHTYDCGGNLIFGGYQKFGCYMSTKTQIMKYFMLPPHYKVRIDINFWKIDDWPSSQLFYIIVDQWTQSWYFTDNTGSNICADTGSDYSSSQYNDFPLHTLNSIIVEFYSDCSTTNFWGLTNFSITLYECYLGCQFCLDSTTDCIMWILWQSFFQLQNLDDGLEGWIKNKFPTFTLQTNDFQLKMLVLNQWDSAITYLTLPDHLSLIIQFRIEYLSTMPITVRVYINDEWKYGLISEKKQVDYRTWVIDDTKNQIKLQIYSVDGTVGIRELSVILRGPINLIQCIDDNLVPFDGCFAKQESCLEGCTFCVKGECLMCDTRWDYNSLNKNCEPSCGDKIITGNESCDDGNDIPYDGCHECQFSCPKNCQNCKFGQCQTCDLGYHYSNGICNRNYDNLPQDQIQVDYNQFSYFIEETEIVICGDGKVQEDEQCDDGNNIPNDGCYNCNFQCILNCQECNFGVCQQCSPTYELINGKCLEFQTQFRFISCIRSDINENQISDVTYCDDYDITYNLEISGIKQQNIVFQCPQYCEICEYGKCKKCLVDYFLLNNLCITQITKGALLFKDELQLSLIEIGCYKCNDSCQIQCLQCQNSYCFSCIQGWQLINGICQQICGDNQVAILSKEECDSNLNDCINCKFVCPENCQICINSKECLICEQPYVEINNECQLACLTECQKCINGICHDNCINGEINIDGICYSICGDGIKQQKEQCDDGNNIQFDGCFNCEYSCPKYCLNCEEGICLECQQYFQLIQNTCYDGCGSGIKSYDEECDDQNIINVDGCSSNCKIEIDYKCQDQTNSYTECQYFESPYMIVQFLNQTYIKYYIQVSFSQAIYFRDNYDLQSLFEFSVNEASSEDFATKLESNYKPVINQILNFQFQIEIEFLFTTNNSLISLQISLLNEVFNQYDMQLINPSEQIFFKQYTKLSQSDVEKTKQLSQYQEVMIIAQGIGSVVVLISGNFQIFVEILDNLQYQSYLKYTNIIYPENLYIFFETTNLISIIPILDLLKISQFYQIFQYQDFIESYAKLKFYNQNAHLITNLQFFVIQVILTFIFVIILKVICQILFRLIHKVQFQHLLIFKLRSLKSKILIKTLNWIYNFFHFIMIFVFQFFQDGGEGLLLANAWDLLFKTFLYLESSSNLDIISRIQTIISYLTLAITGKFIISSLKRGFTRKNYNLNYRYKAVYLLKQFFFCYFLICFQNHSLIQLLMLTTTNMIYLCLLIFVEFNLEKLDKIQIIILEISIITFTLTSIFHLTDYNYLISEENKIILGFAQIYMLISGLLGIFIKQIIMFLQKIKHRCLQYKKKEKVSQRVSHLIFETVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXI5</accession>
    <name>A0A8S1PXI5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1350057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXI5"/>
    <dbReference type="OMA" id="FEFTKYD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="283" mass="33316" checksum="3F9CF7828820A039" modified="2022-10-12" version="1">MSTANDIGLIFSYVYDKISKIHLHNVTQSLSSIKIQLELILGQMDEVTQNLTYSNLEYLFDWAKACQQSMKINFFQNQEEARLMHEINQILLEIFQYFHNAIDKNFMIPQSTICIANELGNIITQEDNFMEVMGNSTLVNFAQFIDVYATPNFKLGQENIDNAQATFYKLNYGALERVFTSENTLSQIIKNVAQQREHFFQRFQLNVTIGNFEFTKYDEQNQFFFYHPLYALNEEKIKKLLIVQFVALNYQPSIQLDEFIQSPKFADNIRKFYQKLINTIQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q0P4</accession>
    <name>A0A8S1Q0P4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1360112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q0P4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8108303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="49539" checksum="E20C9F08EA87E9CD" modified="2022-10-12" version="1">MNSKSLIKINLNYHHIQKEVLQKFMNFDNTKKIGFFQFLVIYLGCTKAEQLSIIILQRIIKCKKYLNMSIKYLLFFNNKTKMDLAIQLESVKEMIKQLEQREADICRIKTLKQQLIRRIDMGDDKWKIINLFEQQFYIMNIIFRNQIIFVNFIFIILNIAFLQKKLKNLWNWHQQLQNQWILIIIGIVTKVNNQDKSKERISKILQLIIKINSNESRSILLQKLHSIFTLFLRNNSAVNLEQIEKKLSNYAEQEQDKFNYYLHLLIKKDNKLIKSINLRFLCLDTQDFDPQLFKLFLKYVYIQKELMKSDSEQQLYLSDLGKFNQNCIIYEDRLVLKKQEVLHIQNSQWQYLQGAEQLNQEQLFKQMAIQQQKYFEYNCYEDIDIIQILNNVIKLQMMHQYKFYYICY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1P5</accession>
    <name>A0A8S1Q1P5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1380064</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1P5"/>
    <dbReference type="OMA" id="PWAGEVH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03762">
        <property type="entry name" value="proteasome_beta_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8108799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="214" mass="23722" checksum="E6A24643881C192F" modified="2022-10-12" version="1">MNYTDILTSKFEPETTGTTIMAVIYDGGLIIGADSRTSSGQFVADRCADKIDYVHDRIFCLRSGSAADTQTIAKYVRYYIDAHAQELGRLPAVATAANLFRNFLYEYKDSLSAAIIVAGWDPYKGPQIFTLPLGGSVIEQKWSIGGSGSTFIWGFCDSNYKENMTLQEARAFVLHAVAHAMYRDGSSGGIIRLLNVTKDKIEREFIDYKDVPVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2K2</accession>
    <name>A0A8S1Q2K2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1390070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8109069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2K2"/>
    <dbReference type="OMA" id="WSIRVAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="111"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="264"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="881" mass="103784" checksum="5B8449D1233FCCB1" modified="2022-10-12" version="1">MDQQQTDIKELLSLYNNAMQIKKRAEKKLIRFTNCLSELDRRNCGAEIIEQINVVRQKIKEERKNQAEQTVRNSYVQLQKQMDQSSLMLKQKLQEKELQLESMDLEYKCKLQQQQQVINDLNLQIEEVKDYNQDQIFSLTQKNQQLLNLIEDLNKQIAELKQILEHYQNEEFKSQQLNNNSIRISQNSQMLQNIKKLGSQDNNQTTESMDQCQFETLASEVKYQETCENFDEEILLGSSNQCAESKELQSPIRIQDQQSNPNQICELNFTIEELKEQIQDLQQEKQRLIDNLKENENKFESFKEKILINEKNLHNQINDLIQQNQDLLAKEQILQSQIADQKNEIQNIAKLNEDLQQSQQQLNQKDLMISTLNEEQEQIKSQIQQLEQQIELIPKLKEDIEHHQNLIIEKETEIQKLKQNLESEQNLNVEKQKTNEILNEKIIETQNTLDQYIQSNSLLQNDNQNLIQEKQVLTNQINLLNKNQEQQKSEFISKIQDLETQIQILSVKRQESDQNLSQQELLLVNLNRNESNLTKQVQELTSQLNEFEIRYKVLEQENNKQQQQIGVLNEQKNQFESQIEELKQQYSKIQQDLHEQKINKERLESIVNDLNKELEQIKTQNKQLQEEVDEFKRNMDELTKINSSKDLQRDEQLKFQKQLENQYKVLEEQLKNAKQQSQEECNKIAEGYKEELKLMQEQLMRSSENIKQYSPVREMSQDDKYKEQINRLNDELRLSKLQIAQFSTVNDQLKQQQKDLVLKLQNMLKLTPAADQDLNDLILIIQSNLSSCQSSPARQSSKRTSMIDTTKMLMNKSRLSIGSNRGSVLVSQNQISDNGYRNSANLQDNGFRNSLNVQKIPSSSQSNTDLQKILDDVSDQDIVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QC77</accession>
    <name>A0A8S1QC77_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T1490141</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QC77"/>
    <dbReference type="OMA" id="NDCPNCS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000039">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000051">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="355"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="403"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="132"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="136"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8111990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="439" mass="51621" checksum="48E161889905E0A2" modified="2022-10-12" version="1">MNSNHNEQLCQFEQQKPKYQHFYLELYKPKLFQIHGPKLKKSDTLKCQQNKGEQQNSQDDPIYLNIQDTQQQEIKFGLKTMEEQSIQKKIKIIQRFLKNYNFNKRFRLRRNDSSEDLEYLDINKDSTFIQKGFKECKICLSYKRMHQFLPCLHEFCRPCVAAHLKENIIRGNVLLIQCPQLQCSEQFESLQIKNLVSYSLYEKYQRFLKRQLISKDKNVRWCPRIDCENYVIGQGNNLLICSCGQQICFKCGSQYHQDMSCDQAMDFQYLQARKKLQVNDCPNCSVPIEKKGGCNHMTCYKCQYEFCWICRGKYSSTHYGVFNIFGCAIPGGQVSNIKPLQNPMLIRVLMIIPKLLLTIILTSGLLIFLPFFLIYLILSAPYRLLKRISNFRMSNYRKCAQFGFFQLLLLSGIILSPITIICAILISPVLLIIYIVERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QG35</accession>
    <name>A0A8S1QG35_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">P-loop containing nucleoside triphosphate hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1590016</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000164">
        <property type="protein sequence ID" value="CAD8114246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QG35"/>
    <dbReference type="OMA" id="WAPEITH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000983">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8114246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="22832" checksum="0D23C747FA116FF4" modified="2022-10-12" version="1">MSEKNPISIKLIVLGDANVGKVDILLSYIKDQISQDFIPKVFENYTITTVVDGKKINLSLWDTAGQETYNRLRILSYGQADVFLIVFSIIDEQSFQNAVTKWYQDVEVPELKNVPKIFVGNNKDQRNLSNPSHVQYEAAKSKIDQLQCQYLECSSQTQEGIKEVFDQAIKKVLQVKGQKSESTSSKNQNGSNQEKEKCLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKH5</accession>
    <name>A0A8S1QKH5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1700021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000179">
        <property type="protein sequence ID" value="CAD8116213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKH5"/>
    <dbReference type="OMA" id="FANKETF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="301" mass="35804" checksum="3293ADB4C47C3495" modified="2022-10-12" version="1">MFANQDCFEVYEKTQEVRQAFSEFKQNVIIQLGKLEFQIETIIFEQFEQSQTNQIDQIYDNKIDNDNSQYIDDETQELVSIWNKANAQQNEEQIPKYNDQLVKRIKKTFTLWEQQIKELLQIDLQIIPRYSSLKYRFDNNAKFPSIKIIEERIIEQTQTQTHYCFALVEQRLNQIETSSIQFKFPKFVGDIGVGICDKKILKSKDFRPFLNALNNGAFVCFQDSYVINTEQEELNWKTKGFKFFENEVIEVTYEPSLKRVIWKKVLKPNEGYSVVLRNHKRELYFCCIVKTQGAKVEIITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKV0</accession>
    <name>A0A8S1QKV0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1700044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000179">
        <property type="protein sequence ID" value="CAD8116259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKV0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31354">
        <property type="entry name" value="OS01G0793500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31354:SF2">
        <property type="entry name" value="OS01G0793500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035775082" evidence="1">
        <location>
            <begin position="18"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="553" mass="64270" checksum="706E0B43E4F98ECE" modified="2022-10-12" version="1">MTKYSLIFALLLICCFGKKTIYDRLHGTHIEEEFDNEVITEITMYDEEDPKLKYEKMMYTFMKLNETQVIGCRKTDHHEFFGMFFQNAGRIGNGGQNNIRLIKYEDDCYESMRVSYETNEAENRVCVTFTPGKYKNSQQCSEFYLIGTTLNYNIVNIKDQKEHRVYFNFRNQKQIEAFKYSGAYIFRTCDYIENWFGDLLMTIELFFGGFSSNPYLGPIFGSHPPDWMVRSNIELIERATGYRWQERPDVVVDLKADEVNNGDFLAVTRFDGLDQIIEWGTGGRIGHSAMIFEIDGEKYVIESQDAWYWPTKKIQKTKWEDWKVYAKNAGFNVAVLPLSPEKRAQWNQEAAMEYWKKMEGQPYGYHTFLFGWIDTPKDNYPKALSAEFATYLFSFIEKIAPGPITSLVGEALNKRLGTEGLSVSEIAIEAAKQGIELGDLYAMVEKDEWVYSDGPSQACAAFVTAMYKAAGLFQPFHIEALEFTPKDIYQLKFFDSNYVVPEKCKKNDPDLPYCQLMGTHRIELEWYNTVEPYENMNERCPSIPPEYIRPDGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNE4</accession>
    <name>A0A8S1QNE4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1950002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000204">
        <property type="protein sequence ID" value="CAD8117303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNE4"/>
    <dbReference type="OMA" id="CFNININ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8117303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="220" mass="25645" checksum="115331E7AA9E3C33" modified="2022-10-12" version="1">MDSIPLLKYDALWIISNIVFETQQQIQIILDNDGINILFLGLESQYDELIELGVWALANIAADNIKFRDMLLQKGILFHLKRIWKQYKKTKSELIKTIVWALSNLAKGKPTTKFSIRGLIQILSEIRISTDDEEQLIDSSWGLSYLVQDIYEIDIQIDKRLIKKLTVLLNSTKHTLIILALKTIGIFQMVMMSKKIKFYKLQFCSLLKYCFNININQQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNK0</accession>
    <name>A0A8S1QNK0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1790013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000188">
        <property type="protein sequence ID" value="CAD8116744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNK0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="86" mass="10306" checksum="17504F82971464B4" modified="2022-10-12" version="1">MIQTENKYNLIKIWHCQLKLNLKSNLSFSIKCVVQNNIKCFCKNLIYKSYINVTIIGTFEQNLLFLKKNIYEIQFYNPKINPKDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNP8</accession>
    <name>A0A8S1QNP8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1930004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000202">
        <property type="protein sequence ID" value="CAD8117249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNP8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="43938" checksum="7D3661C0609EA0F6" modified="2022-10-12" version="1">MPRYQQCQMKYFVKFGTQFKKKQREILICCQFIEQIKILLMVHIDLKYSFHTYPIKKVVASDQINLQKFLTTVKGQTRLVNYILNQLDQEVQESKINKLKKNLKTTQQLYFNFSLRDHHLAIFKLLIYNKAEVKQTIQSYNMSSKFNNIIKSQYTSKFQLEIGILQYKLDIFNKIQYDQQNEKKQLNISHILVLLLVTQNWLFRQSWSHINYNEILLLFQSSFNIVSPFYIRRTSRYFQNVNSFKSSNHGIYQFIVFKLSKVQTLRCRTIICLDINGNSITNQNCTKEMSLLSGFVILSITVFLIDRISYFTKRKIEWYDTKLVNERFSQKSFKLWQSMQIIMNIDCSLIKGIYQIYGLPTETTSIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2024-03-27" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPX7</accession>
    <name>A0A8S1QPX7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1880002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000197">
        <property type="protein sequence ID" value="CAD8117094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="923"/>
            <end position="1027"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1199" mass="137888" checksum="EC7E1EE7236C6DBC" modified="2022-10-12" version="1">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</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QSZ0</accession>
    <name>A0A8S1QSZ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T2690001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000278">
        <property type="protein sequence ID" value="CAD8118876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KFECAVQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="734"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="155"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="869"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1062"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="734"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8118876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1367" mass="162830" checksum="D1D1CA3AB3D62B13" modified="2022-10-12" version="1" fragment="single">MSDDQPEANRLSVKQLAGRFQSMMKAAPVQQQQQPPPRKSEVIAKNPQQQMQNKEETNKVLNKLEFAENEQDSNPRQGETIEQLQKAIENLQRDCNKKDQKIQDLQENLVIMSEQMKSRESKIREEVQQETKETSEFGKERVKLIAELHKCQVDLQESQKQNANKFSQIQQLTNKATQIQNLSKLEIDKLKQQNQELEEKLLQSQQKIDQLTQKIEELKELNSQLNLQNQEVEDVKQKLEKEFQQRYDEVEFEIINNRQIIEDLQNQLKELKALNLQLESASINGTFDMKQQISELQDQTNELQNQNQELQQKLHAKQIEFDQMNKAKSREIEKLKQDKIELQQELEQTKQISEQTQAETESNYKNQILILQDKFQKSEEQISKLNQKIQELSSDLIQERMLYKSNEAQLNGVITQQKDELSQKSSLVLQLTEKIKILQDHSVQQETNISKNIDDYKVLLDQNNQQITQLSEQIKSLKKQQRQQEQENKQVISQYEQQIKQYLAEITQTKIQKNEADNQKQNSESSLNQIIEKQKVQLQQANAIIQDLNNQIEQFQQNIQDQEEQRMSVMSVKQSLLIEKESHINTLEQQVIKYQNELTQKQDQIVLLQQQCEKQKSEFNQAIAEKEKNTKLTNQQYQEFQNKISDLTNEINQLKSLVDKAEVDKEQNIQQFNEANQQLKDQLNSQNSLIQELQEYLKESNSKEQLAIQKSTQQSLEINQLQLEIGKLKNDLSQQEQKQSQTNLENSYKLKEQQTQNENLKNEYKQIQLVQEKLKQENFQLNEEVNDLKIKLQESQENLKQINQINESQKEQLQKLNDQLYQEQQKESVNQTEKFYLQQLIQQHKDQVNEVHEKNKLITSISESKDKQIEALKKQGEDALKNYEQKKQTQYEEMLQLKQKYEQQIHILEDEKKSLIAEYSNRIANLEEINNDYQKNSSIEQSQFNQEKLNLKQQLLDQQVKIEKQLKEIEQLQIQLEEKTQEIIQINLKNEYEILQLSEELVQLKQLNTKQVEEINERDLMVTLHQTQINLQDDQYRLEISNLNVKIENIIKDNQVQIEDINVNHQKQLLQKQNEIQDKEHQIKKLDQQLFDYQAQMEQRLEYIQEKEKELEKLIQNNQALQNDLQQKEQEIIQLKNHSQNLSIELEKFKQYSQLEQEKQQQVILELTENLKQSEQLFKQQNKSLEDQIKNLEQQISNLNQKIAQLQDNLNQLNQKYQELKNEKQLKENEYEKQLQELQHQNDIQNEAIDSQIQTNVEQSELISKLEQNKQQLLEELQDLVEEKKQVELTYKQTIEDLKIVQEQRITEINKKNQDLIQLKNMMLIQKDEQMDELNLQVQQQQEENIKQLNLNDSLQIHIRELKNTYX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QWV2</accession>
    <name>A0A8S1QWV2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T3830001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000392">
        <property type="protein sequence ID" value="CAD8119893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="IMMGWIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="285"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8119893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="37588" checksum="CAFA4A11BA01A97D" modified="2022-10-12" version="1" fragment="single">MQKPKMIEKEKDFFCSKGHKLPVVTILLDPKSSMDQRLLCIECLENTVINSQAVGLKKIVSLIEENQIKKMETVENITKEQIKLIEQLHSIIDQMKSHVMQQLDQLNTIMMGWIQNLQCQLSQSSQYSFFDELEIMFFKSNNSNSNSYIHEILKTNLCWTTKLNPLLEQFNQFTYYNNFKELLSSLDTITQQYTQSYLQQSINLQQKNQDILSLNTKMNIQQSQTLQSNDQPFTYQLIKEYSIQQEGWCGAIAINKDCSTLVAGSISQINVFEFKQRMLKLIQSLSEHNDYVTTLNFMKQSNQFISGSRDQSIIIWQKDQXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JLW5</accession>
    <name>A0A8S1JLW5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JLW5"/>
    <dbReference type="OMA" id="IRIFEHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="60" mass="7044" checksum="536FED7B7052BC63" modified="2022-10-12" version="1">MNFDAQTMLATQIGKKRIIELNIYEENSITVLNYKKTLALRIIELQRSIHKDFGNSDQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMN4</accession>
    <name>A0A8S1JMN4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMN4"/>
    <dbReference type="OMA" id="AFECIFE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035813512" evidence="1">
        <location>
            <begin position="16"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="93" mass="10504" checksum="37868CF3A70A0822" modified="2022-10-12" version="1">MKYLLFLAIILIALAKRHHQPDAVLSCLQGTCKEEFDICDNSTGCLKQLKKCMKVQERDPFNYSAGYECLEQQKTALDAFECIFGKCLGVHGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JR40</accession>
    <name>A0A8S1JR40_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JR40"/>
    <dbReference type="OMA" id="LNTHQEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="110" mass="12926" checksum="E57975097A6A9193" modified="2022-10-12" version="1">MLSKRKVSSQNQEESSNSIEQLCNKIRKVIVSEVGEKYIQNHSEKGVKKKRTKHKCKRQDEQQLIELIQNDPVEVEEQLSSIKAISESQSWVFEELFEEINKLNTHQEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSZ1</accession>
    <name>A0A8S1JSZ1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080305</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSZ1"/>
    <dbReference type="OMA" id="DRIKMRK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="197" mass="22758" checksum="ABF77F0E66E11B18" modified="2022-10-12" version="1">MNQKSNKVSPNQKTIGFRFNCAINLELLKKSTRMALLKMKYQLPEESDRIKMRKRSLIFAKNQLESEKKPSPSKSKFLDTIKTRCSSIADDNNQMPTKLVFQKYSKQLDNINYGNPQIFQSISSQKESCDTLIFPFQRSIYPQRKLVRKNRAETQYTINSSINQPSPNNAKIKVKSLIAIQERFGATGWEVEPSDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVF4</accession>
    <name>A0A8S1JVF4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVF4"/>
    <dbReference type="OMA" id="HEDCFEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="163" mass="19124" checksum="4EF950A6D447B13A" modified="2022-10-12" version="1">MSFSIHQLQQMFEDKDKSEPHTPIFKGKTGSLDKFDFLISQIEELRQQNIEFQNQIQIHSNQLNQKIKNNDVMQTQIKEIEKQIQNEIIQNKLLTFNYIKKHEDCFEIKKQIINTNKQQQIVSIPDLNNPNRPSDCSTAPSPGMKVQIKRAYKKVSSVIFSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JW17</accession>
    <name>A0A8S1JW17_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0110119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JW17"/>
    <dbReference type="OMA" id="LLCQREY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="65" mass="8023" checksum="90DB3A89FBC9BD19" modified="2022-10-12" version="1">MGNNVVITQEQVQIKEKKCHDRYDDFLLCQREYGFNDLKCRNYYLKKYEQCIKKLDNMKLQIQRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXI1</accession>
    <name>A0A8S1JXI1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXI1"/>
    <dbReference type="OMA" id="TPRFFGM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="138" mass="16589" checksum="EDF34983F3664FD1" modified="2022-10-12" version="1">MKNNTQQSTVSPKMTMYKNAMKNKHNQSTQLGEQLQKNQSLTQFQEQQQNQRNKDQEKLKDILEQNRKEIKINQIQSNIQHLDQQQKSFTTQKQSFSEAFKELQIDNKKLYLPIQECKQRFKENVNFDFETPRFFGMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JY42</accession>
    <name>A0A8S1JY42_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0120206</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JY42"/>
    <dbReference type="OMA" id="CDFGTTK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="413"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8047895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="431" mass="50289" checksum="60A06B83D03AD1EE" modified="2022-10-12" version="1">MNQIFINGKSYFIKEELGQGSFGKVYKAQDPSNNHAVAIKVQQQISQHELDLLHKFKELKFKNLVNILDFQICPNQIFIVMELGLISLQDKIQKSKIPFQEARYVMQQICNGLQELHSLGLAHRDLKPENILIFTLQDQAQTQEIYKICDFGTTKDFQNMKTPYIGTPYYLAPEQLVSNPFNQKVNYNQSVDIWAFGAVVYELFTNIPLFNGKTVQEIYLKIMELPIEEEIKKLQHLETKYQNLLINMLQRNPQKRITIQDIKKELSQIQNQNLVTKQGQQATIQKVCQPGNIQMQQTFQRGRQMNAEDLRNPQQNNINPTIKQQLLIAGKNQNEFQRVAQPAFQPANQQKPIFQPTTLQKPIFQPINLQKPNFQPATLQKPMFQPKNQIPIYQHAPQMQNEFLQDNNQQRGRSQHKTHQQKPRPMFLSPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K036</accession>
    <name>A0A8S1K036_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0120465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K036"/>
    <dbReference type="OMA" id="QGAYYIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050313">
        <property type="term" value="F:sulfur dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070813">
        <property type="term" value="P:hydrogen sulfide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07724">
        <property type="entry name" value="POD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000030">
        <property type="entry name" value="Metallo-beta-lactamase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000110">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000148">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051682">
        <property type="entry name" value="Mito_Persulfide_Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044528">
        <property type="entry name" value="POD-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084:SF1">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="295"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="393"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8048437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="474" mass="53343" checksum="4A083ABBD0CA543C" modified="2022-10-12" version="1">MIKILQKFNRTLFPFSKEVQLKQSETLGKGFYQQQIFTGCLAEYAYYIESNKEAIIIDPLRDIQPYLNIAKERGAKIKYVLLTHFHADFIAGHLSLQRETDAQIIMGPRATAPFISKVLNDNEYLKLGKVKIQALHTPGHTQESTCFLLYDEEGKQHSIYTGDTLFLGEVGRPDLAVKSDITQYDLASMLYASLKQKILGLPDDVIVFPGHGAGSACGKNISAGYSCTVGNQKKNNYALQEMDQETFVQLVASDLPKPPQYFFYNAGLNKTNQFQDLNLILKKANTKLTPAQIVSKQGIQIIDSRNSISDGFIPGSINVPLQIPFAHWVGTLLPHDREIVIVCEPGNEEQTIMRLSRIGYDNIVGYLHFDDWKSSGNPINKPTELEVQDFLKRSKLAQIVDVRTKEEWGRGILKHAQLITLSELPQNLEKLDKLRDISIYCGTGQRAKIAHTLLVANGFTRVQYCKVGYDDIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K411</accession>
    <name>A0A8S1K411_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K411"/>
    <dbReference type="OMA" id="TEYYRYH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="266" mass="32167" checksum="647182FDB8AEAA51" modified="2022-10-12" version="1">MFCEHKGNLPELLNMALSSKYQTDFCQFQLHSPIAIQFQDQVCFEEQEEYFKLYQVQLFSYYTYPYLKDNQKHLTEYYRYHRDIPRIFIKNITSQLEVYHEKQREIRYKKIKKQLGMQVKDTTRDDMIQPFKALKGLSELQTSRQLQQLLKQIKPLHYDKSGIATLIQQQKQQMLQSPNLKGLLKMDNIKTTTTLMTTKRTHHTYHTLQDIKKKSHKKQLTLCTDYSPDERNDFYKRWSDVLSHRQLQYHKNKIKCTLLNKKKSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6X8</accession>
    <name>A0A8S1K6X8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6X8"/>
    <dbReference type="OMA" id="YMHESIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="19"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="307" mass="36784" checksum="4CD60F9EB78A8ADC" modified="2022-10-12" version="1">MSLNKENIEKYQTIKNHPAFEKLNYEIVKSISEQLFVIDYRKYGIVLRENIDKQSFMYLVRNGEFKMTKKKSYEQKQLRKQTIEICILIKGDSFGIEHFKDDIQQIYSYTVSCCSIQGSLYVLDLSQLRYILFHHNYFCSLSQLFKYPIQIMKNQIYLLREQQIIQQEKKEIIREESQFLPRKNSTRLRSNSSQFTYADKVGFIIQGQSQYLNNKFLTNQTIDEDKYMHESIMQNIRAMKSNLQESNKIISKSINAVNTPEERLQFIKHDDYRSKMKIMNLLGYKSKQKRQTNYTRFWNFKSLPSNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7G2</accession>
    <name>A0A8S1K7G2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0140138</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7G2"/>
    <dbReference type="OMA" id="FIKQQVC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001562">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001145">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="29"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8049735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="466" mass="54362" checksum="B9992F74ABD50463" modified="2022-10-12" version="1">MDPKSTAFHIYKQTLIKKNNQPITQVYSYEQKDKIGQGAYGSVYRAVHKVSKQVRAVKVINKLNIKYKDRLLSEITIMELLDHPSILRLFETFEDAENLYMVLEICQGGDVFDKVLEKGCLSVDDAFKVYIQYMRAVNYYQGFKIVHRDLKPENFLFQKKNDMNSLIVIDFGIAKRGVDKLQTKSGTAYYVAPEVLDGLYDHKCDVWSAGVVLYVILCGYPPFYGENEKEILIEIKSGTLQFEGDEWQGISQEIKDFIKQQVCPAPNRVIPKDLLSHKLINQYNQKFLNDFKMISMQNLNQWIKYHPLRRLALYYLSTQIDSCDLVNQKNTFFFINQSQSGLITQQELATYLKVNKQDIQKLWPYIDCNSNGYLDYFEFVALTLTPQDYQKQLQFMFDFLSQSDKVITQKSIKFIFDQNANLNNKWDSISNTKNQIAQVNQHEQQINVKNIIDKDIDFNAFKTIMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K825</accession>
    <name>A0A8S1K825_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0130310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K825"/>
    <dbReference type="OMA" id="YQNNIDE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="684"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8049196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="732" mass="86321" checksum="C8A8E560134607EF" modified="2022-10-12" version="1">MIKHHFKLKFPKQDVVQTKSQTKSTFYIARRIIQSTSDRAINILNTKGIDKINNDKKPTPTSSRAYTTSHYRSYSTTPTNLKGQQSQNLDEQAQPFNTDRQQSISLLSSEFKINTQPKSNRVVSLYQISPKEIPKTNIINPILDQEKNYYQSSFRSAKDIQSNKDDLIKINRYHNDLIDENPIIMKKESKKKQSIFINITYQEYERKSKQIFKKHLQGTQKQQIFLQKVLKNIKDSQSEKSESKSLVRKYSKIWKENFSQTQFQEQQKYTKSSQQFKLYFNQFIEKCLNKFRLYQQITEILDLIEKIQQININLFESVHDLRKLYIQNHNIKVSHTEIIEDLKFRYKKEIISFGMIDILTEVKDDEFQSQYFLPDSQGDIAIKPIEYFQLEITRNNNLYQICPIISKLDNHTKNELLSVESLDMDKIIYQNNIDEFVPNIQNNNILLRKFNAQLIYKKYTDILIGNYQNSNDIIDLEHNNYLQQQQQQIPLNLDKKAMMMNLRKQTRDAKIQARKSVLGFQNENLRSQKISINPKYQSNLLVPEASELQLKRRKSKSLQNLSEIEIEQEDEIIKTNESDQGDIISKYYKDKPVKYKQQLRLQNLQIFDYAIKQEHSGIVIVQLMIEHNLLDELIDYLRQNPNFSLNSRNAIGKPLIMIAAQSGNKELVQYMINQNVLLNVKDLDGNTALHYAVAYGYYDIADLLLLNGANPYIKNRNDQNPWNQNQQLLQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9X8</accession>
    <name>A0A8S1K9X8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0170071</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9X8"/>
    <dbReference type="OMA" id="CKAKGND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000012">
        <property type="entry name" value="60S ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8050465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="184" mass="20724" checksum="261368EBDCF7D396" modified="2022-10-12" version="1">MGKQYAREPAVAKKSAKAKASDLRTHFKNTYEVARALKGQTLAQALKYLQDVLQHKRCVPFTRFNGGVGRTSQAKEFGRSQGRWPEKSVRIVLSLLQNLAANAQVKNLSNEKLIINHVQVNRAQKGRRRTYRAHGRINPFLSSNAHIEIWAAERDENVKKEANNKVVARQSRKQAARSKLAIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAP1</accession>
    <name>A0A8S1KAP1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0170196</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAP1"/>
    <dbReference type="OMA" id="HNFPWGK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000574">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="156"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="413"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8050715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="473" mass="54347" checksum="543D00A075401F9B" modified="2022-10-12" version="1">MNYVTASLKDLLPSNQKAVIKFKITPAKKIDSICQCNLKTCMICSRKRSSSMLSLKSIKNSVVVDENSPGLKRTENKNNFVIQSSLLSKQLSRQVSNHQIQNQFYNGKINQESKGSTNSTTWIMTNSHSRKISVDSPIKEIQQQSLNTMGIGRHSFKFLYVVGKGGFGKVWRVEMKSNRQEFALKEMLKTKIISKRSVNSVMNEKFLLEHLKHPFIVNMHYAFQDRENLYLVLDLLRGGDLRYHIGRMKKFSEEQTKFFACCILLSLQYLHQHGIIHRDVKPENLVFDKEGFLRLTDLGVARLNKDSVANDTSGTPGYMAPEVMCRMEHSFPVDYYAVGVIVYELLMGKRPYNGKNRQEIREQILAKQIQIKENTPGITNKAIDFVNKLLIRKPQQRLGFNGIDEILNHSWLHNFPWGKLLNKEIRSLYVPGSIDGNYDFQSQISADSEPSEDSSMVLRRKSVQGLFEGYKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KF75</accession>
    <name>A0A8S1KF75_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CSC1/OSCA1-like cytosolic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0200093</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KF75"/>
    <dbReference type="OMA" id="MQFINTA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF83">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="403"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="553"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="602"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="628"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="4">
        <location>
            <begin position="175"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="3">
        <location>
            <begin position="364"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="216"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8052971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="696" mass="81746" checksum="A5A2249C7670CBFA" modified="2022-10-12" version="1">MNQNKQYNPFALPPDFQLAQAHAKAKQVGYQNQNQQEYCPCCNMAINKIPIGVCEDIIRLQFLGEGIPLFFQSIKLYSILLLLIFVLVGIYNIITTSDLCYKEYHFNLMDNEENQEQGCYHFIGVAYTVNKTELQLTIQAVLALLGTFLVTAGLFLIKWFQQRNLHFSINSSPSDFTILLRNYPYTHEKQQIEQYFMKYFNVNSSSIVKIIRPLNIQDYLNDQKKKNEKLQEQQKIYALMTRPQEVREKNDILNQLMHEIQVLDKQISLFEQQVTLNNLYQRTDYVLVTLQEESLVDQILDICNQNHWYEIIMDYVGCCDKLLYYLNNDKQQWKFINGQRIYIRRAPEPLDIIWQNFNTSHSEKRCKRMLALSLNFLVVLIGAFIVYGISYTQNQAQSSNTFYVKYVLSGLCALAILIINQILEMLIKITSKYEKHTTYTNETRSVMVTQTIMQFINTALVPFGLFIFTDFDQNNLAVSLFFIFLGNLIILPILEILDPLYIIKLIKQNQILKEQDKRQIILTQQQTHELFEGPDYRIADKYSTIYKSILMSMFYLCIFPYGMMMQILSLFLYYWACKYTLLRRCSYPPTINRNLNDSTLQLLGISPILISAGSACYQFVLIKTYSNLIIHIINMIISCLCVGIYLYFRFESKATHTESALSLNYFEEKKKFSTDYEKCNPITMKQAQFQQQYKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KGA1</accession>
    <name>A0A8S1KGA1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0210001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="MEDEESY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4254"/>
            <end position="4271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4363"/>
            <end position="4384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4390"/>
            <end position="4407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4428"/>
            <end position="4448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4454"/>
            <end position="4476"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="2778"/>
            <end position="2793"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="3933"/>
            <end position="3944"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8053677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="4506" mass="517613" checksum="900C8E76F226981D" modified="2022-10-12" version="1">[encoded data, 4506 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KI06</accession>
    <name>A0A8S1KI06_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0210215</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KI06"/>
    <dbReference type="OMA" id="GTYFICF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035740357" description="GOLD domain-containing protein" evidence="9">
        <location>
            <begin position="20"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="26"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8053951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="198" mass="23093" checksum="816251BC6977A5D9" modified="2022-10-12" version="1">MRIYTLILICLINIGYSLSIQINHNPTCFYVQSTENNCQLIVNFQVSGMDENKTEIIIKSENNDQLLQKVNGKEGSLKQTLKEIGTYFICFKSLNRSFKTVSFDFDVDGDDKEYAQSEQFNQMSKELSRTNRNFQSIYRNQNWITDRENSHKIILENTQKSIYWCSITKIGVLILITILQTIFVYKFFKGIDVNSQTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KI93</accession>
    <name>A0A8S1KI93_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KI93"/>
    <dbReference type="OMA" id="REDSNYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001773">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="241" mass="27082" checksum="6689B34202C33061" modified="2022-10-12" version="1">MEENRDLKIIVVGNSSVGKTSLIVKYKTNQFSPQTSGTMGVDMSIDKNVKINNNLYDVAIWDTAGQERFQSIVKISSQNAHASLICFDLSDPLSLDSVASWIQFLKSEGPQNLQIIIVGTKKDLHVQYTQEQLKSISTAWKANFQDEFPIHLTSSKTGEGIQDAFRQAFELGAKAKYDLNIQQQQLQEQNKKSFKISTSQLVRSQQPPSHQKTEAIISNTTTLRESQMNFNDNKTTQQSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIB0</accession>
    <name>A0A8S1KIB0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KIB0"/>
    <dbReference type="OMA" id="KAPFDNG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009240">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02885">
        <property type="entry name" value="NUDIX_IPP_Isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011876">
        <property type="entry name" value="IsopentenylPP_isomerase_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02150">
        <property type="entry name" value="IPP_isom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF0">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018427">
        <property type="entry name" value="Isopntndiph_ism"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="48"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="226" mass="27710" checksum="CA9E43CE9F2F8E88" modified="2022-10-12" version="1">MKRILQHFRYSQQKTQNLILVDFNDKLIQPIDKWEAHSTKYIFNERSFPHRAFSLFLFDKHYRLLLQQRSNAKKTFPLLWANTCCSHQLYHENEDDQVMKYSRSEYYVKKRTLEELNFDIKDTQIELVQKIIYSAKDNDQYGEYELDYIYFAKLQQDQPQINGNPEEVNDYKWLTLNEFLQFKQLNKNSFTPWFNLINDSYLLNWWSKYQENNKSFFSKETIVKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJM4</accession>
    <name>A0A8S1KJM4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0220017</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJM4"/>
    <dbReference type="OMA" id="HETKFQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8054413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="141" mass="16381" checksum="905078597A02C498" modified="2022-10-12" version="1">MSQEKSSYLTPSVRRVLSNSMITLLGRTIIVFYLKGQHIQQFKQLDINLHDYIQSHSFNIKEIQSIFKQVTTSLMLMHSLGITHTDLKPENIVFANQQKYPDQLNDQDIQLNQQTWEVPLKPMNIIAQSSILDNTEHLKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KK05</accession>
    <name>A0A8S1KK05_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0220159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KK05"/>
    <dbReference type="OMA" id="QCFELGT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000136">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF1">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13177">
        <property type="entry name" value="DNA_pol3_delta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22534">
        <property type="entry name" value="RFC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="36"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8054691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="356" mass="40706" checksum="225BAA6A6FFE746D" modified="2022-10-12" version="1">MLQQLWTESTKPKSLDSLDYHSEISEILRKLAKNSDFPHLLFYGPNGAGKKTRVLAFLKEVYGSGVYTVTEEEKEYKINETSNTTTSCTVLSSKFHIDVAPSDADHHDKVIIQKLIKEVASSHQVNSKQTKDFKVVIINEVDNLTKEAQASLRRTMEKYIERCRIILICESLAKIINPIRSRCLLIRIPAPNQTQVAQILDKISTQYNCKISQQLINKIAIASNGNLREAILYLQSTRVNNTCIKDDQNIGAQEWKIHIQQNIVIPIIKNQLVETMKEIREKFYQLLVNCIPVDRIMFEMLQGVLNQYKDHANKTILYELIKSTAKCENRARQGSKGIVHLEALAAEYMTIIKQFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KK94</accession>
    <name>A0A8S1KK94_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0220219</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KK94"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="157"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8054811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="379" mass="45000" checksum="085BEDEBDD482CEC" modified="2022-10-12" version="1">MNQKLQIQLDFDSEDTEGSDISFESSFDEQLEQEFEQTLQQKGIVQFKDQMERMLQLNQEIGTIKLEKANFEDEEELKKLFPPKQSENLISRKSISNIQITSPRQKSQSENKTNSQWYQVSKNILNSSKKVASQPTLEEFQQRLNLFRINNTKSDIVDLIIEEQDKLLKDIQQQEKKNKAIENAVHESIEAKVRKETGDVGYVSFRKKIQFQFRIRKAEEMAQEKVLKQKEKKETDMYRSLQIFDQLFLNRNYDEEGEVKSLAAEELYKKVKKCQDDIDVKNTIIQFIIQHRKKQIDEYKNKPTILKPTKFISEDSREFSLLQPNMQFRQQMMQQQLNKEILDKRDDLEGENIYLLTKINQILEGNLLKLWKHKLGSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKS8</accession>
    <name>A0A8S1KKS8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0200347</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKS8"/>
    <dbReference type="OMA" id="NIDSHYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF155">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HERC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RCC1-like" evidence="3">
        <location>
            <begin position="644"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="644"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="694"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="745"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="794"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="845"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="893"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="941"/>
            <end position="986"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8053472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1005" mass="117255" checksum="0231154192694FA9" modified="2022-10-12" version="1">MGTCLANNVKMSEDYMKNYQNQKQFTYQQKQQVSLEEEPIHMFNIEIDLQNPVFTLHHYDPLLIEDIYQSELSTDGNVKSNFENPKQVQRLKLENPKEFKRVFKELRSQYYTLQNYILPNIKVEQIKNDYYMVSPIINNNNTREDINGVIRQVVDYKGKSLINIVQSESKYVAIYCKIIQDENIRFIDTLQDYNLNKFNKIQQGNMFFYQILDNQIQNNHLPNYFNGNYICELLIQKNKNKPNLLQLKIDLNDGLYLLSYQSLKEHMKRRFMKQYHLWSCFCLSDNRNKPYFACAIFVNYLKKQHIDLNIEEELSRISKGGIMYFTFDRIKCLDLKYQLKQEELNYILSSEIVKEVIQVSLSNTRILMSFLEQKFSIKIKDKIRLIGNIDSHYFTSKAFYKYNNFIESFQTKIESLQSIREKAYEWLSRNNQQTSYELYLPSIFYNLKNIKFYIPNSLILYQWIEYLKQIDCQNIIQDANLLLQIQNTDLVMETQNYDLIQYQLFDALLKGHYLESQFQCNQESQRFYEIAIKISRQCFGDFRNRGSILYPIEAFCWHRLSVFTSQNGDIEYQSVIQYFFSQFIQIPLPCNNIQCHNYKNKDLIQVLRASIKGFMLYDWLFKRFNELQGENIQIFEPQFCYQPGQIILWGFNQNYQLGQMYQLKYKIPISLKSDVMFVKMACGYKHTLAISNTGRVYCWGNNEDGKCGKPSDIKYVEYPHLIESSFDCISIECGHDHSVYVNSKGIVYSWGCGEGGLLGNNNMISTHIPQQVLIPGACKQMKCGGLHNAAIVEDRLYVWGRGDGGQLGLPLELGMEIAIPTKVDIENIQSVACGDAHTIVLNNQKQVYGWGYNEQGQLGLSLDITSVIQPTLLIEDVDSIYAGSLQSYFLKNGNLYGCGCNDDGQLGIDTNIEQKGIQMILNYKIDNIAIGQDHTLCQSQGKVYGWGLNKFGELCKQIQTKPMELFAYKVQSIGCGAYHSGVVIAGTEKYSAYNNEYDYKIQLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWT6</accession>
    <name>A0A8S1KWT6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0230197</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="4">
        <location>
            <begin position="184"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8055474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="316" mass="37780" checksum="B2C0F5ADCAFD5672" modified="2022-10-12" version="1">MKKFFSSIKNATTQFAKGLFNSKEDIQQQENKQDMTPEEQIQQFDDYIKSYLDSHKELKAKILTNRLNFNNAVLELSWRKLLDIKNLYANERRINFQPRLVILEQQHWERIVIQVAELIQSYYDIQTYYDKQELVKIIKQKVLEEQCIKILFSKEEMKDYFQFYEQSDVLLLQLKQQFPQLTFEYSQCQIKEIQKQYLIXNCLKRYCTCFHSGRTCLDECQCKDCKNCAEFAEIRDEAINHIYKKCHRDKKVPATELLSLQMSYGCKCKTTGCQKKYCECFKRGQICGEQCSCEDCLNIPFNLCQKDLNRKKTVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L116</accession>
    <name>A0A8S1L116_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0310208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L116"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="92"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="337" mass="40035" checksum="D3D92CC9164D794C" modified="2022-10-12" version="1">MSQYERIRPIVFQKAPEVKQVRSNSLAVRSSPSKCQLDLSFDLQVNSTTNNANQALKDINNYKKLYVSESCQNNNKENQLSGKHFSFKADENQYLKQRIKQLESQNNNYVTENKKLAHVLDQQIQLNQSLQEQQQSKNQIIKKLEDVQKMNKYQQSNNTDLKQINDQLIISKKVVNDLEDKVQIVLNENQKLSELNERFQFTENQLKIDVEKYKSKCSILESKLKQQQDESKCLELQRKIKKQNDQLEQLARENYQLKQQLIQNNNQISQQDDKQLQYNNNDKYKETIQELECQNQYLQQMIGIDQQKMNNLEDKLSLLTKENYRLTEIVKNRHKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1B7</accession>
    <name>A0A8S1L1B7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0320102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1B7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="123"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="332" mass="39602" checksum="C10B538E5B069AA4" modified="2022-10-12" version="1">MIFDLQPPSPSLSPNSRIHYYQEQLDKAHEIIDQLEIILSEKEIQKQDQHFYSHVPIPPLQFHFKSPSINSQRSKSYVATPQQYSTPRDLRNNMSMVNQSSVKYIEEMKIRHTELVLQFQEDSTEKEQKNKEINDHIQELKTELIYYQEKQTMTKKEKKQSIQQLEVLQNEIENIESQLNKSREQGLQLLQKLQQYKQDIQNIEQEQETTQKDKQSYPQLVTPDLQKKRKFKNSISHKKLMQNLQQEIELLETQKQHYYNYSNSKFQEKSIDTNIINDEIIQSKQNPRIFNLSVNNDSGFKFNTQVDHENESVKSFKINKQKSRSCESCKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1X6</accession>
    <name>A0A8S1L1X6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0250033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1X6"/>
    <dbReference type="OMA" id="WDVNPFA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8056744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="206" mass="23733" checksum="939239A40BEE55CE" modified="2022-10-12" version="1">MLQKATIRFYLSQARKIPTIWDVNPLAYQRMSNALLGAYSNRQFGVLIRENLDMITDHQVAYAFQRINELNLDADEDFYEYVLPALKEFVPNLTKDHSVSIATIIGTCGKINVKDASLWQLFEKKIVADRLYRYIPLNDLVSMTQQVALSDYGSEGFFNTMEKQIGRHRLALSDEQIKLTQEAFERKRLAAPIIKQLKQSAQKQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2W2</accession>
    <name>A0A8S1L2W2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0320180</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2W2"/>
    <dbReference type="OMA" id="HIGANCY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09599">
        <property type="entry name" value="M1_LTA4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.30:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049980">
        <property type="entry name" value="LTA4H_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034015">
        <property type="entry name" value="M1_LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015211">
        <property type="entry name" value="Peptidase_M1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726:SF3">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09127">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01263">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035767171" description="Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal domain-containing protein" evidence="9">
        <location>
            <begin position="17"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal" evidence="10">
        <location>
            <begin position="495"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR634015-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634015-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8061817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="644" mass="74965" checksum="AF36BD8A583A001C" modified="2022-10-12" version="1">MIKLIIFAFLMHQCLTVDIDPNTFSNYLDVRMNHLHVEWLLNLDKKIVNGTAQISFKFLKHTNYIDLDVYQLDILNVYLVNGNPLKHEIQVVKEQSLIQGDRLHIILDRDYNPLENIIIRIKYAYDERARAVGFLTKEQTYSKKVPYMFTQCESNNCRSIIPLQDTPSVKFTYTSTVISTNPLIKAFMTGEQIASLPLIGQYGLESTFYSYSFQLNIPIPAYLIGIVAGEIEQKHIGANCYVISEPFYLDDYAKELDELPFFIEKMTEYIGPYIWGDYKIVILPQSFPFGGMEHPLLTFASPVIIVGDKSGVGTAVHEIAHSWMGNTVTGNNWSNMWIMEGFCVFLERKTYKYVRPQDYDIIEAINGNFNLISAIQGFTDPDQKSYQTLHPITSWKNPDDSTSSVPYERGYQLLYYLEQLIGEENFKFMLRQYLEHFKFQSIDEDDFYKFLLDWVRSNVKVNTQKIIDDIIAIYKPWVYQEGLPPKSIDFKTPKYDEAVALANKWITQGQPQNADDYINYMPNQKMIFMQQFLDNYTKLTHQRLKELDDYYKLSGTKSGPKIAFKWYKTVILCKYDAGLEAVHAFLQSLGVRSYVVGTYEVLIPNYPNQAEAWFAQDKWLYHPLVANRVEDMLKKKIKRAQITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6L1</accession>
    <name>A0A8S1L6L1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0320216</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6L1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20104">
        <property type="entry name" value="MBT_PHF20L1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004092">
        <property type="entry name" value="Mbt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047134">
        <property type="entry name" value="RNF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23041:SF78">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23041">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02820">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="73"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8061875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="257" mass="30796" checksum="122764E5AEC21F91" modified="2022-10-12" version="1">MIQASEQYIEQVTDLQLINKEILKDNFLKKMRNRENIRQNYIEKRKSINPLNYFLLEIKLQQDTRPKFEDLICPICLEIFQKVTTTQCGHAFCEMCIFDSLMRKAECPVCRVKIKTHSFQYCESFDNRIIDLVNQYGDKTQIEHFRNRQQEMEQWNKSKLVESLAINQKVDIMDQQFIWCVATIQQISKKELFIHYDGWGKEYDEFIPLQSNRIAPLGLYTNRDDIPKYQHEQRQFADIIEYINQHGELPTQNVLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBZ3</accession>
    <name>A0A8S1LBZ3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0340084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8062973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NACCLAE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8062973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3195" mass="356289" checksum="08134EE910C178AA" modified="2022-10-12" version="1">MGFYVVLLLEVVYGLSVFKSDQCLECSILIRKEDCEYANCFWSSNGVDNEGTCNDKDSGVDPGNSDGNLSFCKGILDPEQNCSKVKGCAYYNSSCTIFTGCSAYFFHTTLDCQKISYQCISEGDGCIDAKKCDEYLTQDICENSGSSSGSGKCKWDSVVQKCRDELCSEADLVLSTDKECSKFGFGCITKGQGCIESPLKECKTYESTNQDCSKLIGSDGQCEYLQGSQYCQQKQCETAPITYNKNEECDKYLRGCITKGQGCVQNLQPCSSYNKDCTNYIGSDGICEYEENQEICRSRICENGQFNSDEDCNRYKIGCVTNGISCVSSLQSCSSYKGNKTSCLGYIGQDGQCKGIDDNEQQCQVQDCIKDSNSKYVTDEQCSKIQKICKTNGMGCVYILKLCKEYEGSPEICLQLIGSDGKCQGDGTDGKCKRRSCLDAPSTYITDYECKNFQQDCVTNGSGCIDQTSCQLTVKENSCQGTTGCQWNQSCLDSTKCFNFQTQYICENNSAFNYSYKDGITSYYYTKCLWKNNQCLDLKCSDLISDKYDSDFACQQVMENCVYSGYGCIDKSSDCSLFKGSQIKCSQYANKCWNESNATDISPCRSRLCSDNTIFTTDEECAFFQKGCRTNGQGCIEDIKQCSDYYGTQAQCNKFSGYITNSTERQQCYNINGATINTQCIEKTCDLAIGLTNNIDCGKFAKGCVWNGLNSCVKETSNCTSFYGTQQQCNTFIGNKQPCYGLNTNNSNSQCKVKQCIDNTQPGLTDKQCNDFLEGCISNGIGCTPKETTCDKFIGTAETCSKFLGNGFKCNRMSNCSTRQCSDYTNPTKHQDCYNYHSKCRFVNSQSSCINQTLCTNYVAQGCNNTEKLTYCKTIKDDSNFICNYQLGDNNCSNYTCEQMSTQTECESYTKTKKCYFINNLCVSTNDCSKIPIDNTSADKPTTDSSKLTWCNQYKDINNKQCSYHSTLNSNKCSNYILCEQVVSATNSIDCNRLLYSDPNTTNKGCLYYNNQCYSLKTMCTDYVAIGNNDTEKSEFCQSMKLNTSIGNVNTKVIQCQFVIGNSNCSTATCESITNVNNQTDCDNKALNCVYFQSKCYTKQNACTSYSTSTVSSNKPQFCSSMKNSSGENCGYIDGMNNCINSSSDCKVYRWVSYNSNPAPSPVPSSNQDKRIYCQYRRSTQGRLCGYIYGQSYCSLEINYCELIQSPTSQMSCDLQAQGCTYIQQSQLCVTTSRLTNCDDIKFNVTILSTEITKLNYCKSVKSDSGECTWISGSGQCDNLSTTCTSINIETISNDNDKATYCLNRISKAGKCAWRKGDTINTCRSFSCFDIINVVNQATCDKIMSGCTYYQKQCITTQSKCDQYYAYGTNDDQKFNYCQGLSQTTNNYKCTYIIGSSLCTVKTSNCNSYNVSALSASNKQTWCYMLNDLSSNNCAYSTGDSDCKQITCELILSASSQKDCDIYLSGCQYYKGFCYTKPTNCSSYSLPQRITDSVAFCTNLKNTSNQFCGFVSGSTCSDPSSCSSIIASGDTSTQQQTYCQARKSFDGYVCTYSGGTYCTQVSSSQSCTYITGTITNQDQCQSFSSNCSYQDSTKTCDLNSNLNTCNKYGFSVSTDEDTKINYCRSILSGNCSYQFGDTKCSNQVTSCNQISISNINDKKNYCLARNSQNGNCSFDEQNENYCSQIKCSDIESPNSQIDCNKFMSGCIYYRNSCYQTQNNCTLYKTYGIDDLTKKEYCEGLKTLDNTQCTFIASLGVCSNYSNNCSSYDVSGILDKKSWCELLIQIDGQQCTNINDSIKCSLMTYTCEQITANNQIDCDKVLLNKCIYFDQKCYTKQNICTSYVIPQSITNKQLYCKNMLDSSQKYCTSGFTNCGIPTIDCTDIITNGSDDQQKRVFCRQYKSSDGQMCGYLSGTKCEIERNYCSVFNNVTNQEFCDLRLDNCVYIQKSGKCIEKYLVQDCTFMLFDSSLSNKLNYCQYYNINGNTCTWNTAIDHCELDTKKCIDFDASGQTDKKTFCQSKKGYNCSWRDGDTSQFCRQFKCQDIDLAQSQSDCDSKMYGCSFYINSCFTLENNCDNYYALGTDNQLKQSFCNGIPTKSNQKCTYLSNDYKCRLRDQCSTYNVQNISDKVNTCFGMYNSAGIQCIYVKGNTCNQIDKCETYNGLTKMSDCTKLIDINNRLCLEGKTTCLTFNCEDISDPKSQNDCDTKLKNLCYYNNSKCITISDCSTYKKSDWPSNADPIKYCATLIDKNGHYCTADNETSINCRERQCSDKLFYLNSECKEWKSTCKSDGSKCIESNKECSYFTGNKDSCSKYLDSDNLNYCKVIAGTDQDIGSCSTLSCYQNTTATSDSECQSFQNGCLTKGVGCIINTARCDEYRGTKNQCSKFQGYVSKTQTEFCSGDSTNTDQSKCRSRICSDNTIDNSDTQCSYFKTDCVTNGQGCIDINASCSSYKGTQEQCSKFMGNNKTIYCWNDLDASSDQNCIDKQCQHIMGTTNEQCEQAFPKVLINGIQTLLCVSNGINCIKHPAKCNDFLGNNITCQLFTALIDGPCKGNLDNSIGYCQPRQCYEAPKSYNTDAQCYDYHPTCLTTGQGCMKFQSYDQFGKPTLNCNSIVNNDVCSLKLGCTLASQCLVQVSSCSLLTSQAICQTTILSNGTKCIYDLSINKCRDIECSDYVDINDNNICQKYGSNCTTNGNGCVIMKSCLLYYDKLTCQSAYSTDPINRCTWTNNGCRQRECKDFKGTTNSSCKSFLQGCITNGQNCIGPYYQCEDLLQKNCLIDYLGNTCLFYNNICHSYSKCQDMKLTTHDQCQSFSELCTTNEINCIPLNKCENYTNQVSCIIGVNGKCGWINEKCNLFKGCSNVQGTTTTICQQYSQTCISDGIQCVEQNLCSLYNSQYLCEKNKGLDGDCIWTNNTCRLKQCEDLTTNTDTFKKCFDQLTHVKCSSNGTKCISLTSCEYYEEKSCILGSNGPCIYKLPFDQSTGQISCRLKECQDVIGKTLEICKSAFDSLYKKCVSNGSVCIDLNYCASYTTKVACASGGIDGQCAFQPNASKSNDGICKLFTQCSDADNDKDVCLSNSKYCQWISNSLKKQCIPHTCLTYNTKNDCHPVPSFDQKSYLLCAKVNNICQEVQPTTLTMDTCYINSAQTYLWNTSTSKCVQCKIQNNNNVTTTSKVTNNSSLMLNILVIIISLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LE20</accession>
    <name>A0A8S1LE20_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)</fullName>
            <ecNumber evidence="5">1.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0380120</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LE20"/>
    <dbReference type="OMA" id="YGYTCNM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000010">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000266">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836:SF76">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="11">
        <location>
            <begin position="3"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8065827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="342" mass="36781" checksum="E96F51117F141285" modified="2022-10-12" version="1">MSVNLGINGFGRIGRLVLRAAIENNKPVTVKAINDPFMDLDYLVYNLKYDSAHLKSPFTVEKYDQGIVVNGQKIKVFAQKDPSQIPWGDAGVNTVCESTGAFLTDEKAGFHLKGGAKKVIMSAPSKDKGTPTYVYGVNHENYKADQHIVSNASCTTNCLAPLAKVVNDNFGIVEGLMTTVHASTATQLVVDGPVKGGKDWRAGRAASTNIIPSTTGAAKAVGLVIPEIKGKLTGMAFRVPTINVSVVDLTVRIQKEASYEEIMEALSKASQAGPLKGVLGFTSDQVVSQDFLHDARSSIVDSKAGIGLTKNFHKLVSWYDNEWGYSNRVLDLAVHIAKVSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LLJ4</accession>
    <name>A0A8S1LLJ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone chaperone RTT106/FACT complex subunit SPT16-like middle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0420244</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SSRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LLJ4"/>
    <dbReference type="OMA" id="QETQVEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13230">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13231">
        <property type="entry name" value="PH2_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000001">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF1">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="11">
        <location>
            <begin position="209"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="303"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="330"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8068607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="362" mass="42336" checksum="D5D672CB75FF804E" modified="2022-10-12" version="1">MLKSTKYILLFFISEDMLCEVRFYIPPKEQKTEKKKQESNEDEQDEISYLQQVQNQIVNKAKIGGSSDSILTVHEVPFIVPRGKYTMDFFKKEVRFHGNTYQFTTDYKSITRFFLLPMPDEVNLSLVVGLENPIKQGQTAYNYIVMQFKKDQETQVEMKYTREQLDNIGWKGIRLEYTGSMYDIVCDILSEITGIKVVSPKNFKCKNGLFCLRCSVVPHTGFLFPLEKSLLYIQKPVIYIKHDDIKEILLQRITQTTQNKFFDIKIITKNTSYLFSTVDKEELDNLTQYFNSKKLQVKKIQEENEGINKDKDDSDDDSQNGHKVTLSQMDSDEDDEDFQAQEDSYNSVQFQQKVQKTRAANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPX2</accession>
    <name>A0A8S1LPX2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0360299</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPX2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035786963" evidence="2">
        <location>
            <begin position="24"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8064784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="364" mass="41828" checksum="D6A786D2BB3F37D8" modified="2022-10-12" version="1">MKYSLIACLFFCVLNVEITLNNAITTQALEKLKESSWASFIVEFAEVQLSTGGALSELVETINQLISQLEEEQQDIHNKYSRRTDEHNRDVTRIEQEIQDAAKDIFTGQDFIDNVLIPQKERFQQSLIQLKINIEENRRVLNGEIVNRKKQHEQFLSNIAEINEAIGAVDESLGLLSQISNPQLVQYKRVQTNLGRIQSSFQNRQSFAPMIKALLELATEQNFTDQGSVQQLVKIFNELRIQFVDSLNQETLNESQAETKYTERIAQLEKEFAEFQRSVLIKNSELVANEQKLGETIVYVGQRKDDKETLEAQLQAENDNYAAETDLYIRTISEYNKEIEISKQAYGLLTQPSFESYVRSKVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LT26</accession>
    <name>A0A8S1LT26_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0410240</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polycystin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LT26"/>
    <dbReference type="OMA" id="IMISFKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013122">
        <property type="entry name" value="PKD1_2_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051223">
        <property type="entry name" value="Polycystin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046791">
        <property type="entry name" value="Polycystin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877:SF183">
        <property type="entry name" value="AT14535P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877">
        <property type="entry name" value="POLYCYSTIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08016">
        <property type="entry name" value="PKD_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20519">
        <property type="entry name" value="Polycystin_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="658"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="699"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="756"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="790"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="853"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin" evidence="9">
        <location>
            <begin position="482"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin cation channel PKD1/PKD2" evidence="8">
        <location>
            <begin position="660"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="7"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8067936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="886" mass="105693" checksum="198659F2397CCF1A" modified="2022-10-12" version="1">MRKRKQENQIDQQIDNQSNHYQKLQKQILAHQTKVLELEELIEMKQKRNNFIDNEIKLKLRDLRNVEKIFEINKELELNEVKSQNNEQKSNSSTTIYNLQQYQKTLNQKQFLYKQKISQEYFSNSMEKYFQYFESCIGQELNQNQEIENDNEKIYISPMVKFHAIQIQCFVKLLDPKLRNKVNQRTADNQDYVTITFRYSKSDTFDNLKLICLQYWNIIEFDNLFAPQTFNQDQNQYQIKMRKYEFLAPDFTKIPNTELIERYCENFEQRFEKEKYVNAILKKKIIKFQSHQEKGDTYTQANESQTASQFLSRTFKDQTLVNYTIQAEDSQLAHQQLQNSISGDLSLDDANFVISNMILKMKQSLTKSYYSFIKRFPLLQKMFQIDTFELDLRDQKNLKRKGLKIDDYNILVVLLILILLILNCMQYSLIPNQAVIQSHINQIEAVLKLGKNQEFQNISTLDGIFSYFNDFSKFPFQLCNKQSQFNQYYDLIGAIRFRNIKVKEKECQFTVRTDISNRLKCFYEQQNIDTIETNILSEGNKEWMQFKSKNDISINRVIKGKFGEYDQTGYIKDLKKSQQTIPHYRQEITYEMFQNKEYLNVNTLALIITFTIKSKLDSNFFFIELLCENTNLGIHPNSPKIKYFRLANLDKNKQEIQILTYFKLFFSICLSVYIVFVIITFSKEIQSIKSDQTKFRNIITYFISLIFLMNILVLYTNFVSISQAIQLLSNEQNPKNMIEFEDFEDFGNFAQTYQNYFYYLGLCVAALIFRSLLFLGIFESMLNLIESIQFQYLQIMISFKILLLILLSFCMIMRSLQGPYSSEFSSFDTAFTSLLQMVMNSYDVRKLLENNQILTTLFLILFYFLLTYFTFNMIIVVYIDSYRQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LT97</accession>
    <name>A0A8S1LT97_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0410265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LT97"/>
    <dbReference type="OMA" id="IIFIELN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="147" mass="17286" checksum="857F320A82F7373F" modified="2022-10-12" version="1">MNKYRVSTHPLINQFQWKISKRLEQNIDLSKYSTQFCTLLRCNNEELDFKIPQIIGIIKLGQISRLAFLEFKLLIQQSLIEAQINGQFQNDLDFVRDIIIFIELNYLQSEDEKFLPDSMPSSLSPLKAKLMTTKTSMRKDGWKKINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZE1</accession>
    <name>A0A8S1LZE1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-ubiquinone oxidoreductase 21kDa subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0460221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZE1"/>
    <dbReference type="OMA" id="ATIWGRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053229">
        <property type="entry name" value="NADH-Q_oxidrdct_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019721">
        <property type="entry name" value="NADH-UbQ_OxRdtase_su21_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34062:SF1">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 21KDA SUBUNIT N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34062">
        <property type="entry name" value="OXIDOREDUCTASE 21 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04750)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10785">
        <property type="entry name" value="NADH-u_ox-rdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 21kDa subunit N-terminal" evidence="1">
        <location>
            <begin position="35"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="151" mass="17571" checksum="2A48C66BF0B27A7A" modified="2022-10-12" version="1">MSTYRGTFEHDSFLGWLNLFKIRRLQILYNVGERPPYPVIISKPTVGDVLRNLNKADFGLFATVAFLGFFAARKATLGLTTTEYIRQRGFSIAWNSIMMAGALFACMNSNNRLTGFVDNGLQWRRKEQRLTKYDFTSEFEEGTIWKFFRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZJ3</accession>
    <name>A0A8S1LZJ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0480013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZJ3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="27916" checksum="6C4C636FAB95003C" modified="2022-10-12" version="1">MVSFVVFQQRVFKQFSPLRQLSFILLFAIFNFLKMWDFFMIFLFLTVKQFSEEGQRDTQSSRVKPQNFKYETVQTDVQEALNKILQFFVYFSSLSSKQLNGIWVMTLGQKIEEIILTVKDILEVVIKDYFIQALILDQFFQFMLNLSSIQNQIRLEYPKSFQFCQKQDNNIQKKNLNVKTLNFTNNGDFKKIEVVQHSLGMFLLQQILFQKLIKHKDCFVWELNQMIQQDVQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZQ1</accession>
    <name>A0A8S1LZQ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sphingomyelin phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0460268</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Converts sphingomyelin to ceramide.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the acid sphingomyelinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8071043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZQ1"/>
    <dbReference type="OMA" id="VWSQTRK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011160">
        <property type="entry name" value="Sphingomy_PDE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF57">
        <property type="entry name" value="METALLOPHOS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000948">
        <property type="entry name" value="Sphingomy_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035799843" description="Sphingomyelin phosphodiesterase" evidence="6">
        <location>
            <begin position="21"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="7">
        <location>
            <begin position="23"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="558"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="558"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="569"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8071043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="581" mass="68467" checksum="2F3CA2929C9D0791" modified="2022-10-12" version="1">MNKYIILLILCSICFSSSLSQQEKFFCKQCHKFVHTIQKVEHFWDKSIVWFLELVAEGYCSYAKIEKHSVCKGAIHEMTPVIFEGIQTRFLDEDFICPLIRWCPKVYEELKLEDYVSNILKDKPLNTQWPEIDGTDTIEIIHVSDIHTDLFYKEGSAQNCDEPLCCREGFKLKDYNPKKAGYWGSAAVCDLPERTFEQFVNFLKTDVINPNKKTFLFWTGDNVQHDVWKQGREYNIINSKRITEIFKKANLGIPIIPQVGNHEMFPVDQYDYMTEKDSNLRVEFSNMWRDWLGDETAEFFKKNGFYAKEFDNLKVIAFDSQICNPDNWYLLKDPTDPTGFLEWAEQELKDSELKDQAVYFTAHIFTQECMVPWARRFNALVERYAYIVRGQIYGHAHGEFYNLYKDQNGKPMNIAYISSSLTTYNNKLPSFRKFIVDAKTMIPLNYYEYRLNLDKYNYIGKDAILKWDIAFDFLSEYGVTRMYPSDLYAITERLFTEPELVKKFDFNQNSGTGLSGDTAQYLYCLLNNSIKDDLKVCKGETLTKDWTQYISPGWRKRIDTSGEKEEKKQKKQQKKQKKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0A7</accession>
    <name>A0A8S1M0A7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0500257</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0A7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="151" mass="18452" checksum="3C523213F90DCC16" modified="2022-10-12" version="1">MMPFVLSEIQIKISKQFMVEDMLNQLVLSKFYIKSLYLPLQYYIQFILYKQEKDKLFQFYQFDIQLQQSNVINILDIFSLKCTINQFQVIGRKKSQNWNQYFYQFNQKGTNDMKEQRIWKTLSSKKQQFQLVTQIVKMILKLDDVIFCDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M7B7</accession>
    <name>A0A8S1M7B7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0550182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M7B7"/>
    <dbReference type="OMA" id="MRYHLET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="162" mass="19431" checksum="E16127BE5C394CE5" modified="2022-10-12" version="1">MYMRYHLETYEPKSHQKHSDLKLNVHHSHTSSLPLLLPQQLSPMKEKSPQLSKMGEQILKQWGFFPDIALTTRKEIYTEHIKQEKKYQDKFDDLLYNLNTQRPKCQKNHDFNNDFNLKKSVHFNNVVTVKDMDGQVSQKEIKLFSRNKRRQRTKLENLTVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9N5</accession>
    <name>A0A8S1M9N5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0570106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9N5"/>
    <dbReference type="OMA" id="INFASEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="836" mass="98038" checksum="E9DC87CD35F86A61" modified="2022-10-12" version="1">MANTKQNMRPLRPNFNIKNTSQVSEQEPPQPTLENKEQKAPEPKINLRPFVRPPSSKKDDVQQSQQFQESIMDQKVDEQKNTQQRRTPVNGFRKFNLQSAAPIQQNEEILNAAQQYQGPAINLFANKEKIQQEVSQIKQKELRQMVQVQMKQRPLNAPEMNLFSGKESVDGSDTFKKMKPIKPTNKQEPITKDTIKVAELLENKIQFQAPQEDQQIETKKIPRTRVKQAVQEQIDIEQLNNNQQQNTKQQGEETKMDKTQKEMNKTISKKTGKSNYISQQSQQLNEQPEETNKQFVQTKEVMQNPQQTVTTIDDYKERTIQFNTSTTKNVFQGKTEEQKKRVKELQQLITLEVDDYSNQLVIQPRTEYETYLNHIKANIIRNSCDQAFDDRLTVDTQTDEFQTNDFGGQCPEDYNRTFLAHKQQYQKDNRLCEFIEQACDIFEEILNIDNNRNEIKFDQEQLNHKFPNKINNVFKTKCLNIVAHQINNNQLELFETYYIENDKFKWLNGSLIIQYPQNVLYYTPSKVTCLICEQNLLICGNADGQLCGWSINDIGYNNKYIDKIKELLQNEFKVKSINFASEWNLSILDQFSDANMNQLSQIQQITHNSLIKLIFEIVVMDVFGNIQVWEIQDLKYNEQEKLYVDLGIKSKKKLVLLNTINKNLTKIYSCMSMDIDTILICGPSKVINISQQRSYYDDSDMQPCTISNIYYDYFCVGYSDGHIKLFHKDFTKSIWQLRIGLKKIIWIRILSDGLIGIIDSEQNIFVYFVQDNTQMIGKQLNDKDHWRYNYAYQNGQELTIVGKDKNNDLQLIKERLELKGTKSEIFDNIYLDYYGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCH5</accession>
    <name>A0A8S1MCH5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0590071</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCH5"/>
    <dbReference type="OMA" id="FALGMMK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04515">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052969">
        <property type="entry name" value="Thr-specific_kinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763">
        <property type="entry name" value="ALPHA-PROTEIN KINASE VWKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763:SF1">
        <property type="entry name" value="DUF659 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="6">
        <location>
            <begin position="847"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="184"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="329"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8077917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1070" mass="123218" checksum="79E2799C0DDF091B" modified="2022-10-12" version="1">MNQQTPDTLNLQRNEPQIQNQEPQNAPQSPGQINQFQFQTNYGPGPGMTQGMLKIGPGMGPSSAGSGFLHPGMIPPSGMSPLPPVTSPLPPLIGSHSAGMISTPGIGPPPPGMIPPLGMIPPHPGMGLLPPGRIPPGGMGPPPPGIGNAISSQGVNPTTLAYPQSQNLYNQGQSPQQMIQQNIAKNNDEEIQKLQKELKKKNDEIKKFQTQINDLTENLSTESEKNKEELGRLERELKRKLDTSKIQFNDDKLQLTDQISEFKIKIQQLEEKERQNEEIKKKNQNEINSLTNQNTNLEQQIYSLDKINTQNQQQNKIYNDENCNLKNQIDQQKNLIKGHEIKIQQLNQQIEILNQIKSQQIKDQSYSENNSKQFAEYNTKIDQLTKKNKDLEERIDKIVNDKKILETNFEIIQIENKMLKDRTLFFEQQTKELNTKIEQMLQQNSEISKKENLEYLKSLQQQQENKKQSFQEQLKEQQEKLVQKRQQDQKQQKLLNEMGKQKTKELLSKVGIIQCCFVMDIFKQNEKTRKAIIKATQTCCTSIKMTTNRDSVWGIVAYGYTKSGLQIKSQQFTKSQEELSQFLNKQKLSQDQKDQPEDLKSALKEMLTLNWTEQHKLAILIPYSPCHGKKYHHPKKYSRFFGFWTVNYDNKPDDDMESIIKEIIKRGIYLLIIRFNEDTNVMCSQLEIIYSSLNSPHLFKTLSVKGLDLPSSDHDLVLNIQLMASFIIGTANQTTKTIVNEQIRMQNFEEGLNDMKKLKEDVIMLNKDLQKEGQNQKGDLPEISIEDAVEDQKKFEENVKRAKDCTDGALQALCRRGDIANFQSQVQCEDFNCKVYTVELKQASLKDKIENIQSIKFEESDFDLIEQAQWQCIRTKNPFALGMMKKVFLMKKKNNTEEIYVVKIPIQQGTYNSLQDAVNDCRSHLIAKNMMKIFMEKLRSQNDKIMMVQYTDFLILEENKKKYWIAERFFQGNFKKYNNNDGYICEENSELNAISQAFSYFTYQISSFNYIINDIQGVDNYFTDPAINTIKGNFDETDVGQDGISNYLSTLQLRKDICQQLLKSIDIEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MIC2</accession>
    <name>A0A8S1MIC2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0560121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MIC2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="125" mass="14545" checksum="331F04FF4595EA2C" modified="2022-10-12" version="1">MPSQQISKLTDDLGNLENYNLTSDRIENTKEYYQRPQISMKQILRRNQKNPDEIDNQIFMDESIISETEAKLNSLYGESISIQKNLLQNQKSNPIKVINAIKKMDQIKSIINNVNANKLMNKFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJL5</accession>
    <name>A0A8S1MJL5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0630247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJL5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="61" mass="7296" checksum="5258829FDA2C5C0D" modified="2022-10-12" version="1">MIRYSISQVKIQQTPYFQRLIENFDSALKEVNTLNILDHLHMILHLIVYYQTQINECLMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKJ2</accession>
    <name>A0A8S1MKJ2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0580047</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKJ2"/>
    <dbReference type="OMA" id="AQDEFQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="290" mass="33671" checksum="89DF3A4C51D99807" modified="2022-10-12" version="1">MNLKLFSQAQDEFQTETISFNATLPIKNDSKIEQNNNNTQYSQFVPKKSRQSTSMVFSIQAQEQFDLDELTKQPYFKEDKLNTKLSKKTNQVHNTPSIKQIQVDPKFYSQNKTKKRRILSENSAPSKPIIKIIHPQIITPNEFKYTKKSQAINSDSSAITRSNQNVSQTSKILTLEDKIHRLSQQQKATLNNESQKQSQLELIKEDHTEVDDEQESFFFNTGKQIEKSKFHHFLKIAPLPKRIVTFSSVIKKIGDIEICIGIEGKQEITYFRRIKSEKAKLYNHCNIQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNE6</accession>
    <name>A0A8S1MNE6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0680025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNE6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="43" mass="4680" checksum="ABEEDD6216BB76D8" modified="2022-10-12" version="1">MEITVTRSDAKSEKIHSKLGGDGPFGSQSAEKAMKKLVNFIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPB5</accession>
    <name>A0A8S1MPB5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0690008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8082897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPB5"/>
    <dbReference type="OMA" id="IPPSNWD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045203">
        <property type="entry name" value="RanGAP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46761">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46761:SF2">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="58087" checksum="F8539FD809B96D05" modified="2022-10-12" version="1">MQKQEQKMQKQEQQQTEHSTFLGKIVWILIGILVAFGIKLYINEYQNVNCIINGDQIIMENSGMFIKDSGVAYLSSQINQNIDATKLIADLSINQISDLTNFFESLGNLTKLKELKLSFGNNPIINQESQIIKLVETFSKLKELQHINLGMDAILNNQSRFKIIQQISKIKELITLRISLISCDLSEDGIKSLVPIAEIQNLKTLELILVGSKLKEKEMDSLKLILQKLPKLEKLNLNLYANKIQQDGVNILSVGLSQQIQVRELGIDLYFNNITQNGTQSLMKVVGQMNNLTKLNLGLEFNYIKDEGGIQVGNTLTQFKYLKELSINAATKNFGWIGFEAILLAIEKLPPLDKLELIIGVNKCGAKGAELLKNILYKHNKLKSLKINYIENYIGDAGATFIAEGIQYQKNLEDLHINMNFNSLSDRGALELARAVKNAKSPKIVQLKVSQNQLTDKGIKDMLQLLEQSLSKLDNLEVEILNTALTNATRDDIEIKYGNVEKLSLKLNTIPPSNWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQI9</accession>
    <name>A0A8S1MQI9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0680129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQI9"/>
    <dbReference type="OMA" id="KIGNSDH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="25192" checksum="98786F9532B200DB" modified="2022-10-12" version="1">MLNTSSMNLGDTSKVYKQSNQLGILSTAISKIGNSDHSFHKGVKAANTTNVSPVRMRLFRESPERKQRGLDGLVVGEFHPRKQTQVVYSSIHQSSSPMIHKPQQKQQFQDSPTRAKQKIDRFKNWDSKLDIELQLQNTGITRMGNDIVYQSGPQINYTKSIQFNERLNRNNPSISQSDIVQLTAKLNAIPRTELTNFTRGHAHELSTLQQSLQRILKSSKAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MU06</accession>
    <name>A0A8S1MU06_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0640169</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MU06"/>
    <dbReference type="OMA" id="ILHKHNG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="380"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="460"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="501"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8080796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="581" mass="66493" checksum="4591B3D1A67C34DF" modified="2022-10-12" version="1">MSTMVCTTKGCQHKTLCSDCIVTHPKNHLAAILPLRDFDPSSYSVPKTIAKDLSIVRQRMSMLQNHLIQTREIRKSECKRLQKHIVNYFTDELDSFIKTVILQIEQFYNEKHTKIAEVTQNCEEHINKLNSIINGEVDDSRKFDFLLDLQEKLLQVIVPTLTKQAEDLSRSSISSDILINQNFEKAISVVIEKAFVVSNILNTEKKPVVMDEPEYDSLTIFKVNLKNQQKNLDFYKSAIQPYLHTDLLGKEYKVPQSSDFIPKTVPKDTPQLNGLPFEIFISQQKHLNTVVQGHLDIVTAVCVLNLNQIASAGGEGILKFWDIDSQICLGTIEAHKGEIWCLCAITDDFNQDIAEKLVSGGNDRLIHIWNVQYMTLEFTLVGHLDIVKSLCYLRKFQYICSGGGDYRIKIWNRDKCFYTIEDAHKKIVRTIIQLDDDNFASGGDCFIKIWSAFKGQLKYELAHGDDVYCLKELPNQMLASGGADRIIIIWNLRGGFKAHKLQAHNETITCLGFHKYLFSAGLDKTLRVWEIKDQATLVKSIKVHTEQISCIAVNQYRELIITGSWDKRVRIVSLEYVIQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MX84</accession>
    <name>A0A8S1MX84_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0680079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="33"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8082661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="124" mass="14632" checksum="55CF976C9A2ED145" modified="2022-10-12" version="1">MQKQFEGYNNLEEYFLEVEQIIGNNVNPYDIKCPICLNVYLDPISCDSCMNHFCKKCYQKKQSTKCPLCNRLGETRKAFPLLRQLLSQLKMKCPHFNQGCQVIIDYDXIIQASTLFYFQLQFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXB6</accession>
    <name>A0A8S1MXB6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0710241</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXB6"/>
    <dbReference type="OMA" id="IEEDSHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="251"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="358"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="436"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8084229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="565" mass="67082" checksum="7549E913B75DF480" modified="2022-10-12" version="1">MSMQLTQKPNPFFQVRQHSQNHNTNTTNCQKMRMSIIDQISPIKGQQQKNTQRDSFYQVKGQSGSTVCLLSNKTFPSRFLDSLYTSLNYTQESNVEKKNATLPKGLKNNIETLKDHFKFVNDYLDDFKQQFDKVIQTQIDQVIQVLKSASQLSNVLVEENTQIKNQLSNVDIDKLQSHLEQQEQKISQINSENQQLKCQINKLQEENKKLKDGAFNYDKMSQDPIIKNDKENLISNNNKNKYTQRELSYKFEQAEIKLLTLQISNDQLRQQLQHYQLDQQTISQMKETITTLELKSQSLLNQKDALQQENDLLNQKIENLEKQYKDKQKTQEYLIYQNQLENELIKFKQEQAQSHQTIISLNNTIQQLKEQMNILRTQKEQVENSYKQELLQKQNELQQASTRYQVLWQEISEMKDRLEQRKIEEDSHYMLSNAEKVLFETQISNLNDKIKEYEQILLHNDQKIKQLELQLNQIQQEKENYKQQLEKNGCQSSKILDLQELQWKINQLNQIINKKDKELSKKSEELTQIKQGYDSQMKKNQNLEGKILQLMEQEIKINNNYIIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZT0</accession>
    <name>A0A8S1MZT0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0750136</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZT0"/>
    <dbReference type="OMA" id="RLNMTHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07834">
        <property type="entry name" value="STKc_MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000815">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000098">
        <property type="entry name" value="Mitogen-activated protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="33"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8085918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="422" mass="49473" checksum="DC7A513EE5B34D2C" modified="2022-10-12" version="1">MDASYNYDQYAQKAYSANVFKVDESEFIIEKRYKPTQQLGSGAYGIVIGCEDTKATVPEQKMVAIKKIERTFEHRFYAKRTLRELKILRNLRHENIVNLITLQLPKSRKHFYDIYCVTELLDTDLKRVIDKEHAKLNQDHFKLFLYQILRALKYMHSANILHRDLKPTNLLLNKQDCMLKVCDFGLSRALLQTTKTQQQNPNIMTDYVETRYYRAPELLLGLKTYTQAVDIWSVGCIFAEIVRGKTLWRGQNSKQQIKMIFETLGTPSKSKIMQVQDTFVSSKLIELVSELGTLERVPWDKVVKGLPPEGYDLLEKLLEIDYKKRITAAEALKHPYLKELHNPSDEPTRSPVSNMEFEFEMYEFTNEQLKDMIYEEILLYHFPDFKKQYEDKITNNQSVINHILKGENAKVIDPEADDDYPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0Q0</accession>
    <name>A0A8S1N0Q0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dual specificity protein phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0760040</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0Q0"/>
    <dbReference type="OMA" id="FPSKYSY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000217">
        <property type="entry name" value="Tyrosine-protein phosphatase yvh1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948:SF2">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE DDB_G0269404-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="64"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8086170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15786" checksum="997D1829FF6DD7C4" modified="2022-10-12" version="1">MSEIIPHVYLSSIVYAKDQNWLTKNQIQNILIIGDLPQYFPSKFSYKCIQIEDKPETNIKQYFEECIEYIDSIIAQNKNILVHCYGGQSRSVTIITAYIIRKLRLNSLRALNYVKQKHARAEPNQGFLDQLKTFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N3A8</accession>
    <name>A0A8S1N3A8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0760163</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N3A8"/>
    <dbReference type="OMA" id="QRMYPEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="115"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="258"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="149"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8086402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="759" mass="92289" checksum="CBB2A3F5A87BFF4B" modified="2022-10-12" version="1">MEDRALRTNQIINRYQDYQTTKLGTPGDNHYLKKENWKQIEEKNKNQFRSNDKQTQNFESKTHYQQAGISLRDKEQYNNNTNLKQSYYYEQTKPQTANYQQRDDRQDQLQINIENRQYRDQQIPHSSDKYYSKQSGLPFCNRTPMDPRSNFNSKTPQENDSQNKFKSEYSYSGFKTDFKWPEYQRMYPEISSNKNNQQEKTNLIQKEQTQDQRGLLDIELRLQQRREQAQKIAGQQDGRLNYYSKSLEYSQRQQQIGLENSQVRMKQFQNELRNEVQERLKEQSNLQVKQDYRNFELNSQRNKFERELDNKQKKNDILELNQFEEKLNLNERKNNDYDNLKKDYGMNIRGQQFDEQTSFQTRFNGDSKYQQVSDLPKYHLGKRQELDSHLKYDDKSKYIDRSLKHNKSQTEDIIRYDLKSEGNIFRESNDRLRFKEKLNYETKQHYQFKREDNYEKKQHYHLQKENDQSQGVQRRLDLYEKLQQYDQKNNKKYYDGHVFQVEKLQSNNNQRQETRLNEYKQSIEPTIGYSIDKYEIKKHDQEIQQIDRMEFKRSQFQPEINQIKIEFEKKYGQFNPETSLKQSEFDKRRVIEDKFRPEPIQTKIAGAREYLGQKYYQRDPYRYEQRNSYHEESSNQDRLKLDKGNNKININNKAIEDILQGTALNKYIEKEKNQSKMMDRVVSFTSSKINYQKSPVNDKEERLGYDRLNQLQRGLGNDRYTLNQYSKRLPSDQNLQRYGNSSGLIVKQFKNYDTIDRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6Z1</accession>
    <name>A0A8S1N6Z1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0790119</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="MITEICD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="268"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8087762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="396" mass="45200" checksum="E7BA9F26821CB2A6" modified="2022-10-12" version="1">MIALLIGMPXKQKKIEFNIKIAENLNGKLVQNDKQRIIQLLVNILNNALKFTQEGGCISLKITEGDLFSIIFKVKDNGIGIEEPKLSQIQNSIHDTIEFGAVLKSHQGTRQQGLGLNIAAKLVQGLIESQDNQLIISSRKNQGTVVQFKVQNLLQNNLLQTHLFTLQSGKFNQSVDQFELKEPKIDDSKLIKSDNSRIEIDREDQDQNSETYKNTSRLNDVEKQPEIFLPISPEYFSDKFIDFNQHLLKQKDSFFLKRYGSICQKCCHVLIVDDIPFNQIALKMILNNHQIEADQAFDGFQAIEKVKQKMLQHCSTYKLIFMDIEMPGIDGFQTSKQILELVSNKQIIVICSAYDTQENLIQGQKLGITTFLQKPVKKDDLEVVLKKVFQNETNCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N8E4</accession>
    <name>A0A8S1N8E4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0760125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N8E4"/>
    <dbReference type="OMA" id="WAFSTVW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF1">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="85"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="361"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8086336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="374" mass="43468" checksum="C3C72CAB8C9709F0" modified="2022-10-12" version="1">MSKKDKDYTGFPGDCNQTQLKALAEFRNIVNGMGLSDKVYDDPYILRFLRARKFDINKTQIMFNDFIKWRKENDVDNIMTYMFEELPQVRTHYPHGYHKTDKIGRPIYIERIGMLQLNKLFEITSEQRLIKYYIQSYELLLKRIFPACSQAKGTRIDQTFTILDLKGGSMKMVSKQVYNFIQLASNVGQNNYPEILGKMYIVNAPMMFTGIWAMIKIWLDEKTKNKITILGSSYKDELLKHIDIDNLPDFLGGNSKCENTDALSLNIGPWNPDGTKPLFPVEQPAQQEEETQQLNQTQEDEDQKQKFDQLKSALADMQFASPTEKAPHNPNKYEVTTQNNHMVSDTPLNTEVGEEDNQFSQQQQQQLQQQLQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N997</accession>
    <name>A0A8S1N997_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PiggyBac transposable element-derived protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0790147</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N997"/>
    <dbReference type="OMA" id="ESEFWLY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029526">
        <property type="entry name" value="PGBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13843">
        <property type="entry name" value="DDE_Tnp_1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PiggyBac transposable element-derived protein" evidence="2">
        <location>
            <begin position="399"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="891"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="908"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="941"/>
            <end position="951"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8087799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="981" mass="114194" checksum="75A2981D081EBFB2" modified="2022-10-12" version="1">MSEITSNQPQLESQFLYELVNQQQPQSNVQNQQSQHANIDPPIKLTHDQLQQTNNLDHIIQLLIQEDNQDPQDQNHHSSPQPIQNQEHQQPEDLILSHRQDINSQVILAALESNDEDKKSQEADCKLLYKKQRQTSIIIDDDDIQDDLTNSKKTTKNEKNNVPPPQQDPLPITQNVSEKQEVVLPPPSQRSSEDVPQLEIQKPFEDKPSTLEHITKPEPALKKQQKEFFSFTQPITTTTSSQKKNKNPIVIDSDDELPQPQTSNISILKQKIEDTTPSSFFRKKTTENQPPIEIGENDNFQSNQIDKQQKITQLLQQPIPPPQNKPLNQQQRKHQALLEEIVQEDDGIVQDIENQDWTLFTQLNNMIAPKFSGQFRMLEYKDNTQDMIRLLFGETRFKELMKTCQTNESEFWLYLGTKLIMGYMRLPDINEYFYGEDWIAGGGIKSIITQQQFEDIDQRVEIIQPKLQTESQLAVKLEFNAQTFFRQEYLPQFTQELNDRFKKLILPGQELFLISNFYTLIYSTKIQWYQLIDKESGIILQQFFWATPMNKTLDLNNHRDLQKRLRMMFEPYHLGRHIVYSQGLLNAESVLQLYQQKIFVCTDLMQHSLLPHPPTGDQQVFYSSKNKEPIYLCYKNQQWSTCVSIQSLQQRNQYLQELSQMQFAEEKIAIQPSEPENTSINSTQKIIFIFVIESILHNIRILKKQEIKSFKTELALQLINKIIDYKNPKPLLRKDGSLQVDRSCQTDQTVGIVNDQLYHCPIHNGNARCQVCLSKSILSKTTASCLGCNKVLGTNIFLCIYPCFRLFHLNPKLYLKEGMYYQIVCGYDQYDQDQEDEEVDQQFLDANLRFKPDTSELDSIYAKSYQDFKNMIPINYEDNYHKKTTIVVIQREKGGNRRGGGRTKASRQQQQSIQNQGAQAEQGNQGDTQDTPKKVRKQNKKSINTPQQQGQTEKETDQASNAMFDFFSQVRKQAYKKQSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDG4</accession>
    <name>A0A8S1NDG4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0780042</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDG4"/>
    <dbReference type="OMA" id="MYVIMSI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000982">
        <property type="entry name" value="Inactive ATP-dependent zinc metalloprotease FTSHI 2 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000311">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF97">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE YME1L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="193"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8087125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="416" mass="47041" checksum="ED55F23E1DF62E48" modified="2022-10-12" version="1">MVDFRKFMQIVMRKFNDKLNVSASAAMLIAAFCLYKAKAKPALPLVKISNFILVLQKKLVEEVIYDGTHILFRCVNQAQWLSTQAEPMIKGKELLKALMEYGVPRFQNSTQIYYSEKQMMYLIMSIAAISYTFGIIATNFINSNKISNKSLLAQSTGVKFANIYGLDHAKKQLQQIIEYLQDPLKYRNVGARLRRGVMIYGPPGTGKTMLAKATATESNVQFLYCSATEFIEIYVGTGPKRVRELFKKARELAPAIIFIDEIDSIAYKRKNQNFGAETGGDNERVSTLNQLLTELDGFKENENIVVIAATNRIQILDEALLRSGRFDIKIEIKLPSENDRKGIIGVHLHNKKHQISSGMIELVAKNANGFSGADMENITNESAYIAIEKQQEYIIDADIQEALKKITLEKQHMRDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEJ9</accession>
    <name>A0A8S1NEJ9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0860087</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEJ9"/>
    <dbReference type="OMA" id="ENCFLKN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="370"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8090542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="434" mass="51033" checksum="7657094856DDFB1D" modified="2022-10-12" version="1">MNRSQEDINSRETDKELTKEVIRICIFNCVCQFYMLIFSCKLIWQFDSKYVIIFAWFMDLVTFVIIMLDRNIGKVLSLVYFLEKVLSISFKITIVIHFEVQEIKLYYVPIAQLLITIGLFLFQVLHHDLSKRKHIIIAHSYSILYVIQLLLLTLKWNKLFEYTYYQTFIIAWTALGINTFLIVLLLITMVENCFLKNIPQINGTIIFWFVFYIFGLTILPFFLLRTICQFYEEETKQSNILLASIIVLVSILIYLFLFIFFTLKIRKKLIVVLQFENRQNTIQKIPSNTNATKKWRKLKIPIMFIRISASYYKMLTRQSSIDTSTRNTSSIPICSDVLRPDSLNFRQSPFQTIGKQMKPKPKNQDSGDYCLICFENEPDVVLHPCNHGGICNSCSENLIKTTKQCFLCRSEIRYALKFNQKDGEMLEATDVQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGX6</accession>
    <name>A0A8S1NGX6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0890067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGX6"/>
    <dbReference type="OMA" id="YYRAKHF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="132" mass="15059" checksum="88D2B70F4C6CB0E2" modified="2022-10-12" version="1">MNLSARSYMPTQKSISPLIDRTTKKRTLTIQELTKSTKTANKIIKEKSTEKKLTSNSSQETSETCLTRNDSAQDNNRLQQLKIRVLTEKVQQIEKLDVCKTTLLLKELQYYRAKHINSSNTSVMVALALARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJI3</accession>
    <name>A0A8S1NJI3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Heat shock protein 70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0830014</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJI3"/>
    <dbReference type="OMA" id="QCPVIGI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10241">
        <property type="entry name" value="ASKHA_NBD_HSP70_BiP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000720">
        <property type="entry name" value="Endoplasmic reticulum chaperone BiP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000058">
        <property type="entry name" value="ER-type hsp70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042050">
        <property type="entry name" value="BIP_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035907916" description="Heat shock protein 70" evidence="7">
        <location>
            <begin position="19"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="625"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="270"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8089145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="651" mass="72234" checksum="8DC459E5E40E26BA" modified="2022-10-12" version="1">MKKLLLALLLVSVTICESSVQCPVIGIDLGTTYSCVGVFRQGHVEIIPNELGNRITPSVVSFTDQERLIGEAAKNQAATNPSRTLYDVKRLIGRKFSDSTIQYDKKFLPFEIIDRDGKPYIQVEKGTQSKVFAPEEVSAMVLSKMKEIAEGYLGQNVINAVVTVPAYFNDAQRQATKDAGNIAGLNVVRILNEPTAAAIAYGLDSKQVESNILVFDLGGGTFDVSILTIDNQVFEVISTSGDTHLGGEDFDQRLIDHFIKLVQKKYGKDVSGDKRAIQKLKRECEKAKRRLSAAQEAKVEIEDLVEGLDFSEVLTRAKFEELNSDLFRKTIEPMQTALNDSGLKKSQIDEIVLVGGSSRIPKVRQLVKEFFEGKDPNTGINPDEAICYGAAIQGGLICGEKSDATDGLVVIDATPLSLGIETVGGVMNKIIPKGSFIPTKKSQVFTTYVDNQTTVTITVFEGERPLVKDNHKLGQFDLTDIPKMPKGQPQIEVTFEIDEDGILSVSASEQSTGKKNQIQITADNKLTKEEIDRMIKEAEQFAEEDKIAKEKIDAKNSLDSYIYSIKNQIEDEKKLAKHLTEEQKQTLKDAVQKSEEWIKKDEANYDKEDFEGELKDLQKVCDPIIQEAQKKKDENKENEDDADADDTKTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NMU8</accession>
    <name>A0A8S1NMU8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0850221</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NMU8"/>
    <dbReference type="OMA" id="TINLEMN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8090345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="132" mass="15612" checksum="951E9A043F1C5D76" modified="2022-10-12" version="1">MACKLNYKNLLYEMSRFQFILLSMKRRTEIERVHNIGIKVPIQLSESGDSYTITQGQVNQLKTINLRNNNQSEYKYDILTINDALILSYDYKNFEQSILSSTKRSANENNQIEQDFFDINQIEPQYNQNEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQE1</accession>
    <name>A0A8S1NQE1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0960016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQE1"/>
    <dbReference type="OMA" id="KYDAGMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025982">
        <property type="entry name" value="SieB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14163">
        <property type="entry name" value="SieB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="105" mass="12449" checksum="78243077509514E1" modified="2022-10-12" version="1">MLQQEQLFIVLIVLVIIAIIIAHLITKCQGREKEQQVKDRLRKKFIKNNIETLNDMNEEEALLLQETFKNTLENSKQGKIAKYDARMQKIQEIRQKTVKFILTHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQF1</accession>
    <name>A0A8S1NQF1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0550109</name>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T0650206</name>
        <name evidence="19" type="ORF">PPRIM_AZ9-3.1.T0930186</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="12 13">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQF1"/>
    <dbReference type="SMR" id="A0A8S1NQF1"/>
    <dbReference type="OMA" id="WVPRSVN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000005">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="15">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="16">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="408"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8075855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8081378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8093609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="442" mass="49456" checksum="B080F67A714B016A" modified="2022-10-12" version="1">MREIVHIQGGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGRYVPRAILMDLEPGTMDSVRAGPFGQLFRPDNFVFGQTGAGNNWAKGHYTEGAELIDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGSGMGTLLISKVREEYPDRIMETFSVVPSPKVSDTVVEPYNATLSVHQLVENADECMVIDNEALYDICFRTLKLTTPTYGDLNHLVSAAMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMIGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMCAADPRHGRYLTASALFRGRMSTKEVDEQMLNVQNKNSSYFVEWIPNNIKSSICDIPPKGLKMAVTFVGNSTAIQEMFKRVAEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEFEEEGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTA5</accession>
    <name>A0A8S1NTA5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0990197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTA5"/>
    <dbReference type="OMA" id="NPMMEMD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="138"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="376" mass="42956" checksum="2866933FA6B11E99" modified="2022-10-12" version="1">MSKQNSFLANPLNDSLSDFNAKDYISDNQPNSDNDQEDCPKNPLESDNEENEGNESAVLQHLIDNDNNSSKSDGVSNPMMEMDPISLENVINQNLASIGQQYHICQTILDCNKTIELQQDQNPLYSCDELLDPRQSNKEKQKDIIKNYESQLQQLQKENQLLKNQLIERETNFSEQTKQKDNEIATLNKLNKNLINENTQLKEDIQNQKNEIQSFKQKIHQLQAEKLKYHRSKTGAETTRGYYAQDLQQATSLNDQTIATLSANHLEIRKIPTLSIDIPCPFSAKSKIEKRNQFTFKNGKSDANHLTTEECQTQRQQKIPKNQRSLQDLIHKAQIGIAFSKSNTASLSKAQGQGQLFFESKSTKNNQKNTQLVPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUF9</accession>
    <name>A0A8S1NUF9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0970153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8095029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NUF9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="139" mass="15572" checksum="00F0C6716B4AEF96" modified="2022-10-12" version="1">MPQAQRKDLNDSITITPGPGQYQIPKIRDSQIITMKGFKYNPNNSVHFPGPGQYNPDDSMCKLKDGSVKIAPEQRSKALLTQYVPGPGQYSMKSTLEGPSWGFKKEIRSTLIKKDFNPGPGSYNIPPKFNDVPKYLLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NVA3</accession>
    <name>A0A8S1NVA3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0870107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NVA3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="283" mass="33484" checksum="2D45D44FCF7C6DF8" modified="2022-10-12" version="1">MFKFGKEKFTKKSLPKNFADQILNLEMEIELNDQIQIELIQDLISLYMEGVEYYESIKDRRHLYFQRKLNALMLKPSFINATTKYEESHKPKDDNQIKRAKFQEQTKRIELDLIYSQTESKEAQVKGIVDNHTNQVIKIETIIKNEIANQSDAFQMRLERRRRSKLTHSLSQPEIDLQSQKSGNSNKKKQIELHEQVVQIKQIIHEEDEIAANRKDSTLQKTQEKKWEIIHSSSIMQENKKRTNSLIQRRRSVFLTSKQITSIKRSWSCGATKHLEEEPPKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NVD6</accession>
    <name>A0A8S1NVD6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0980103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NVD6"/>
    <dbReference type="OMA" id="LCSAQTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="173"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="659" mass="78353" checksum="CF4A03136EB3B712" modified="2022-10-12" version="1">MYSKNLIDSDFQSNVINQELKPFVQKIQDRQKRIYELSKQLEENIQKSLSINSIIFERELRNKQKEMRKIKLENEKRVTKIKKEYDLSENSNLSKLLQKQQEYISNYEQLYQEEIQFIKFIDQQTKNSSYYTVIHSILNDSLIFSGQKLDNSSVLEYLTGQNKHKIPNFHSSIKQIIQQLQNIEENLQVILNSNNHDSQLLLLQQSLSNFINILKTTQTQIDELLLEMELIQKEIQDSDKIRKNNIILIQSIIKENQDQKELEIQEYIKMNDNLIKQLFSSIEINRKYSIFQQQSNEDLQKLSQLSEIQLQNKFQVENKKQNKQGLVQLKKVPTVLLSRTRIYSDHKKDLNDILNLSNDQSLEMAGRRDIMAKESSFLYQSQQTINTEKIIVNSVLSKQFSQIYTQSPNSTKYIEKKSHYIPHNTTYKNIAHSDKKTALPLKCACPPQCLCSAQTIMQIHQCNCQLYINSKGYIMCQQCQFSQQIQDYGFYCPQNRQKNKFKNYKDFFISFWSHLTTLTVNEQIVEFWENLQVNCQQQWIQYKQIDFNDLTDEVSFMNFIAYCPLNCKCYHKTSQQQIHFCQSPLIISSLGELVCKKCNFFGSLQFHFIQCPEQKEFYFYQNIDLFLNSIDLNHSNFESEKQNQEFLQKLRKNLIKKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NVK9</accession>
    <name>A0A8S1NVK9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0930164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NVK9"/>
    <dbReference type="OMA" id="YKIMLIP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="320" mass="37501" checksum="767BA2BCAAC2D988" modified="2022-10-12" version="1">MSQIEKQIQKQRKLSHEFEQTYNSTEIHSNLSFYDEERSQDFYDDNSLVDEDFQDIWEMSSICTENSLNPLSIEENPKNKRIRVSPHPELAMKRATSNQKGINNNVAESITKNANSGKGIAIHVLGQGSIGTQSKKDQKSQKLLNKQIRWNNNNNNNNNNNNNNNNNNNNNHDFHQQQQNQYQENNSYYGQSLSLQPQQQQSESHQEHQEDKQIKQSQKITLSYLLMEREIEKQAKIKAINFIEKYMVKCNVDEVVQMISETYKIMLIPDQQLQQLISVLNYEERQFQFNLSLERLSLSRNVHPLASKADQKLRKFFLSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6F9</accession>
    <name>A0A8S1P6F9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1070110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6F9"/>
    <dbReference type="OMA" id="KIHETEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="74"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="314" mass="37137" checksum="8FFB764E754DC4C1" modified="2022-10-12" version="1">MFNYLLEFRNQKLLVQALSNKIHEIEYFQKTGKPYKYGNNEEFIIDGQQLIEFYFYDYMMLQGFPNEQSQLKKSSDIIQLRQRLINDKYLSEMAILLNLQNIINVGNQKSLKNNPKILSDCLKSIIGAHYYDKQNDLETLRDLIHPIIVQLLNKGEQIAKAQWKYNPKSSFLEYLNSLKGQLEINPKLTIEWKKDDSMQNSNKSIYQITLELNSTLKIQKFGINKRETEQNVYLEALIQLRKYDIKHNQNQKTLNQQKTKIIQIQDESQFLTSLNSTINNQDLTIHSFSDQNQDKYNLDCDLQFNLILEKIAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8J8</accession>
    <name>A0A8S1P8J8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1090095</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8J8"/>
    <dbReference type="OMA" id="VSTHHKN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000170">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF75">
        <property type="entry name" value="CENTROMERE-ASSOCIATED PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4 5 6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="3"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="695"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="371"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="723"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="758"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="83"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8099335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="775" mass="90568" checksum="50A9FACD1BF480C9" modified="2022-10-12" version="1">MENIQVAVRIRPDPEPSIWAIHDQHIFLNNQMKKPPNGSGRTTFAFDHCFNQDSKNADIYSKEVKQLVLGSTNGINGTIFLYGQTGSGKTYTMLGKENDEGVLLQSFKDLFTKIEADLNKTYVLRCSYFEIYNEQIFDLLKQSSKLQETLQVNEDQKKEFYVKGLIEQSVSSINEIFDVLKRGEINRHYAQTAMNHNSSRSHAIFRLYVQSITNNFIRQYRREQSQHKFPTLEQLEAENNNQLKGAMVTESVLNFVDLAGSEKVSNHFEDAEIQDTNRRVKEGQYINKSLFFLTQVIYLKAEDKGHVPFRNSSLTKILKSSLDGSSRTLIILCINSAALHFDYSISTLRFGMNAKKIETKVLANINYQNDDEALKILLSDYEKKLHDLEKNRIEQKEGFEQQLNQLQNENQQLQNRITNQNAQHFKCIPKLYKEITWADRFKYDLHCDGAGDILVTNKKVKKPPQHDCQGKLVLSALKVSEQNRKTLLSNYEIIKNQYLQLNNLYNQSKVKMQQQNEQIQYLNIQYNKLYSYASEMKQQLNFDVKQLENTQLEQMIQFYQTQLLKLQIEQQHRGQFINLDLKYQYNIEQFKLIEQNQNELDMYQEYQQINLDEIMYNNLVNVEMVKEKEDEDQSVQSEEFFKIEFPTYLHHSNNITKQTMNETASQLTVQDLSEINFKTVLQSFISEMDVLQPSVSTHHKNNSTSCNKKQTVKYKTPDKMKKQSTSQTTIRGKQNIEPSRQSFNNIPKPKIRPRQSGNANEELSLLLNNTEMSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PDI6</accession>
    <name>A0A8S1PDI6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1140061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8101004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PDI6"/>
    <dbReference type="OMA" id="SICYAER"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035812946" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8101004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="23092" checksum="6A566F5231DC4249" modified="2022-10-12" version="1">MNQLFLFISILGIVTAIPKKFMQIDESNSNENLIDNKITQKQNDIILIDQITDWELAKQAFVNQLQEIYEYCQVILLNQELVGLILVLIISYSFYLMIKQEDIKLKQKSKSLQQQQQRKLISNPQTIQILSDSLEQKNQGLPQMSTIHGSICYAERVNKTIEELIVNEKGILQRSSSHPLLNKQDQSVEELNYLLRDIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PED1</accession>
    <name>A0A8S1PED1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1130144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PED1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8100878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="187" mass="22221" checksum="C62BC58C39E50F90" modified="2022-10-12" version="1">MAINQDPNQKHYYVNKTTTLSDVKLYDNALKEIQFALKIDSQYHDVNQAFNINHLHQQGDIYINICQSKQYEKVEKACDQVLILDSQNQYVLNCQQEIPKKISKQQPQVSQVQISVQYQLKEVASRITENLSFESHKFSFGKRPQIYEHQNEKLKYILNKNNKQVVKQHWIKFLPFNRMIQIHQNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PH58</accession>
    <name>A0A8S1PH58_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1180114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PH58"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="194" mass="22078" checksum="E1C55F3DBF49EF5D" modified="2022-10-12" version="1">MLRLLNQLLENLISFGVDFVNQSFLQVILCYFNIKYKSIKTLKIKQHFSYIQFKYKQQAQFVWECLASLREGEGKGFQGNLKVGIISSLRNLQGYGHMSSTNQIPSTAGQIGSECSMQIKTVLQQLLQLKLQIDNWIQKILLHLEAHFNTKKLTKKAVGRKQPPKSGNAIPKDLSLYFSSKSEIIVISQSVVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PI40</accession>
    <name>A0A8S1PI40_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ZZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1190126</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the MT-A70-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PI40"/>
    <dbReference type="OMA" id="RRTHKHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000426">
        <property type="entry name" value="Putative N6-adenosine-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000029">
        <property type="entry name" value="Putative N6-adenosine-methyltransferase with ZZ-type zinc-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF2">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE MT-A70-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="7">
        <location>
            <begin position="531"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="7">
        <location>
            <begin position="594"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="64"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8102867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="713" mass="83252" checksum="3CC5274BA82A8065" modified="2022-10-12" version="1">MTDQEKSYDVKLTSDTQKNVQKQKTGLKQINLDNLVYNKRERKQTGIQALQPMPIIHHNKVIHNKQGRKKKNTELHTPPREEVKQNQEPTQIKQLSISCEQLVLESDNFSEFDPKIKPKKSKKFRRTHKHIDLEDSSDEAQYKSKLILKRVNKIFKKSYEQKLILSSGSEQENLVKELFKNIYVKIFQDIPVTAAELKQLNQPECSTGQEGDCKLLEEEPKQDEEPLRILTEDGTDFETKRNLKLLGIENQEILSKLGEIKSYINCDIRYFNIDFLVEKVGGFDVVLMDPPWRIKGGQQNDSSFMFTNSKFSLDYNTMSNQEIMDIKIEKLSKKGFLFLWILNTQLNIAYEMASKWGYEIVDQIIWVKLNPQGNNVYLSTGYYFMHSFEICLVGYKCPPGEHVEYHSKISNNIIFSSVRNKSQKPIELYEIIELMMPGSKKVEIFARNHNLRHGWFSIGNQLGETFQKWLVQISCNICGISLQPGICRYKSKKISNFDICQNCYNKKINDGELTENDMFFLANKADEEVLHQYHQCNGCDQNPIWGARFECVTCDNYDFCEACFDNLLINGDPIHKDHEFKVIELPTFAEGIPCHDSKCNGCFQKPILGVQFICKQCTNFSLCQNCFFTRTQSELNGQRHKADHRFEPIYEVQNYQKKTYKCQGCELEKSVSVYKCENCFGFYFCEECYVLKKDDWKCYVATSHKNYHTFIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJ56</accession>
    <name>A0A8S1PJ56_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T1190162</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJ56"/>
    <dbReference type="OMA" id="LESKMLC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000029">
        <property type="entry name" value="Kinesin-like protein KIF17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF50">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="6"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="706"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="352"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="93"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8102929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="736" mass="85341" checksum="B36B815BEEA9E5AF" modified="2022-10-12" version="1">MKRKECVKVVVRARPLSSKEIEDGRKRIVEVDTTRKEIIIQNIKGDGNEAQRTFVFDEVFDMNSQQEQVYHNTALPIVESVMDGYNGTVFAYGQTGTGKTHTMEGKNDPPHERGITPRTFDHIIKVIEGTPNIQFLVRCSYLELYNEEIRDLLSPNHLTKLELREKPEQGVFVKDLSKIVVKSVAELNEWLKAGRANRKVGETKMNQESSRSHSIFTLTIESSEFGADQQQHIKSGKLNLVDLAGSERQSKTQAVGVRFEEAININLSLTTLGNVITSLVDGKSQHIPYRDSKLTRLLQDSLGGNTKTVMVANIGPADYNYDETMSTLRYASRAKKIQNNPKINEDPKDAMIREFQEQINKLKDELARKAGGVIGPNGQIQKIKEQVIEVEDDEELTELQQKAQKEKADLEQQIMQQRRQSTLQEEEKKQLQLKLKEKQKLEHNLKKEQENMHKKLQELQEQLLHGDKMKEESRQKEKELLKARMELEERSQQARRQAEELAKKEAMQMEQEQKYNSTKEEIEAKTQKLNNLIQKIKELKSQAQEQTQFRQRQKEELIEDNRESLRQLNLMNLIIEHFIPEDEEKRLKDKLEFSEEQDDWIIKEFEANPLKKPTSAFCFKRPICNFSRMAINFGDANPRYRPENILQTDLDLPQRMTEDFSLEPSQKIQEVLNVALNEDEEEQQMIQNEKQANVYIEDPSIINKEAILNPPSNPNKVRLQSAIKKNARRPQSGFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKI8</accession>
    <name>A0A8S1PKI8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1220022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKI8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="9848" checksum="3E778D06F72D502D" modified="2022-10-12" version="1">MYNNHIELIGDDQFVNFLMDQNQYQFINKDQIFHMLIVAQLCNVKILMKMIQISYFLYINYLNKLKERNNKKRKNQKKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PL06</accession>
    <name>A0A8S1PL06_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1200099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PL06"/>
    <dbReference type="OMA" id="DLMHEEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="90"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="332" mass="39392" checksum="7EA118716440301F" modified="2022-10-12" version="1">MLTILHFHCQISQVQHIFTLYDESIEIKGPQFLMELDINYKMKIRWEISEKRILSLQIKDLLIQANQDDLIQLKQHLDGRVIYLGIQNIYETMELISAKENSKVCQLEDRFTKACVCCKALRKDAYEIEQLYQSIMILYKLQQYSYFPRIYEIYESKTHLYIVQELMYQHISVDLMHEEIQIIIYELLKIVKILIENKIYYSKIKLSKLMLDKKGNLRLIGFCPAQQTNEIDVENYLDKIGNIMMNLYGVNDNFNSLPKIADNGNEFIVGLMNPNRDYRFSYEDAMQHEYIKNIFSESNLIQHKSPSPKLITDVTESLTFYSKKGSCFQKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PN36</accession>
    <name>A0A8S1PN36_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PSP proline-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1250047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PN36"/>
    <dbReference type="OMA" id="MASTHTY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007180">
        <property type="entry name" value="DUF382"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006568">
        <property type="entry name" value="PSP_pro-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052584">
        <property type="entry name" value="U2_snRNP_Complex_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785">
        <property type="entry name" value="SPLICING FACTOR 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785:SF6">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04037">
        <property type="entry name" value="DUF382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04046">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00581">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP proline-rich" evidence="2">
        <location>
            <begin position="303"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8104747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="511" mass="60354" checksum="0D1F66980F8B1004" modified="2022-10-12" version="1">MKAEVTKSKLSKEEYEKFKRAERRKNNKSKKKCTKEKQEIIEEVVKGDEEQIIYDEETFQEIEIDALITGKHYDNFKQVFDNFNQHTERLMMKTYEKVYENEKEKEKEQEEQQAAEQQQQQQQQQIQQQIHEPNQTIQNYLQQGKKKEAKLSKKQRKQLRWLKVAELKQKVKRPDLVEFWDITSPDPMFLIQLKSVRNSVTVPRHWSQKRKYLQNKRGILKEPFQLPDYIEKTGIAKLRDPFVDRDGGKMVRQKLRERMNPKQGKLDIDYQILHDAFFKYQTKPIMTKHGEIYFEGKEEEMRAKNFCPGKMSAELRSALGCTDFQAPPWLPNMQRYGPPPSYPHMRFIGMASIFAEPSSYHTSVKQVIEDSCKKADQPGLYATFKYYDEEDLNELSQKTGLQADAPVDKSLWGKIEEVEDEGEVEQEEEEEPEAQQRQEQQFDNQSYQFGDSDTRLPGRSISGLQTPDVDLRRNLQNDPRPLYQQLEQVQVDPSSGNQIFMTGHTYVLPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PNQ6</accession>
    <name>A0A8S1PNQ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1240109</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ININCFV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NACHT" evidence="6">
        <location>
            <begin position="1439"/>
            <end position="1604"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2204"/>
            <end position="2245"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2246"/>
            <end position="2287"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2288"/>
            <end position="2329"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2330"/>
            <end position="2371"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2371"/>
            <end position="2412"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2413"/>
            <end position="2454"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2455"/>
            <end position="2496"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2497"/>
            <end position="2538"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2539"/>
            <end position="2580"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2581"/>
            <end position="2622"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2623"/>
            <end position="2664"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2665"/>
            <end position="2706"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2707"/>
            <end position="2748"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2749"/>
            <end position="2790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="539"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="810"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1275"/>
            <end position="1374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8104641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2872" mass="335570" checksum="6BCDF1915A22C525" modified="2022-10-12" version="1">MKQTTSTLRGGGCGSHRIISHSQGNSKSDIQDIEIFITQFDYYVEKICTKAAVAANQLESQEIMIAIQWFIFQEENIYKLNKSTEKVMKSYNLILEGIRKLLKSCLIYIRTDLFKCLYILQFTTSLSKVILSFHIMNDERFMKQDLQKEFLDISEELTQLIEIEKNDLIQNQMELYLFLTKTSFQMAPNNNQDRQDFLRGCLSGIISSIIQMKPNAELLKSLFQGACLIHKMYAVQKNRKLYEVYYQIDMLQWEIINYFKNDKQNDLEGIILQIQSIHENLVKNSNNWKHHFLWIQMIGKILIYNPLLKKQQLYHFNNIGIKQRQIWNEYLHKGLLIQMNHSNDQAVILLNQLQNKELLQLDRLILEDCFKEWKNFLLLKDFLLNDQNENIHFTFSSYLKCKLEVGKIEPQKNENIMAINQIQQFLTFILSDKLITLIEDNYEKLGIVVNIYNNIIKNNNQFNEKIQQATYTSKIIKMIQNFKQYFINTQQIIKIIRLNQRKKNYQIEQLDQIMIIEYKNFQQLKYLMKVLEQILQPLIQEFKDNINDLKIQSQNQNDEIVERERLLEQKQYIQVELKKLELQDKKNSLLDDLQLKLEAIPTNPLNKKKIMESAQSLIQFITNSWKQLEKREISLLQNNNHLIQLFENQLNDIYIQYEEKIQEIINDNEKSILFMKQIISIKYDIEILLKPENLKEIKLIFDDMHFIKSIQNLRFKHLQLHLKLETIKKSFKNILEQTSPQELQKLQELINLDEFLLSVIKEYPKRIICCNSNIEKQIISELISVQFTEHDYQNQLSKQKGLIEYLEFRLSIEEQTISQEEQDLELIEKELGELFVEESPYQLRLETIIEGLAIDESLKNVIEEKFSISEQRLEKDKFNNFLSQLKKIKDSGKYQNIDQIKIEYWDQLAIQTTTVIKTLETFGYIDKEIFQMLLNEELMNLKRLLSEEKISSEIQLNAALQSNQEKMINEKDKITITTKSDQNQENQLHDLIVKQKEIYQINEKDVITWMKSNFFSNLNRRAYLLFLIKVVKLQIQKIIESKELLQRFLEEVNVFSDKLDQIQKYKYEVQTKLQQRFQCRFQEFIKNFEQLILTQINLQQIEDENFESYFERIQTNLSKGNYDTEITQTQQNISDFLKTLKVYFQEKLLEPKYSLNSHIKQEFLIECIRKLYLLDNQEVEYDEKSDQQLGLFDTLKQKYHDFQNNEEWKIKQGLVFTIIQISSNCFTDTIISFCQKSLIQLWVSEKDLRVRNLLKNKNLINMQMQILQRDWQTQHDRIAGEMQQMLKKIDQLQEQIFHEANLNKRDLQLKQIDETTLQLDEYIENINEMGQQLQLITDFVNHIRKGLLRVEGKMNEMKESLINMSNEIKLLRGKSVEQLLEIRKWKVLMEAAYKNVRSIYVPLKTQEKGKNELSNLMNLEQFDDKIGEVNKFLQEEKTVLLIHGIAGSGKSTTAKKIEEFIWKQHDKNKQVPNRILIPIYISLPSLNNPNFQAIEETLRQDDYGFDDIQLKECKEMLEKKEFRFLFIMDSYDEMKLENLQKNLYINNKIKQNWSDPLVIFTTRSEILTSINYIDWFAPEDKTKLKEIQLLKFDQVQKQEYLKKFTLQSIKMLIFEMYEQQIQIENRRAMDINKFELFWEKLLSNLKFDITQMKSETLLNQKQIEKIFKFLKDDGYITLKSKDFLRSLSINLQKLWSTEKYDNMIKQINLNKMVETPQMMEILVQVLPEMMVKATQIVNLKQNFLKNFSKMIKEYFKSQFMIHMYKWQQNKQLTNKIEIESQLDTFVNQDEISEVTQIDIQNLEKIDYHQVSLEVWNKMEDNQIPLQLQISQDFGELDLNLLKIIEPNLLLPNTLFNKVKIQKERIIQVVSDALKEYNLTSYDFYSEFINYYHLKQIEKQQNLGKSINIDSFLHDLKQYSIKLAKSMSNKQVTQVQYKQQGLLYKEEGEEEKWLNEFFNDDTQYGSYKKDIRSCSLIQKKGTNFLFVHKSIQDFLIATDLYEMLALSTDLDLQILSSIIERLSKEQTQNQDYLEFISKLNLSELNFKFDTLSLFERQQQLNDIQKNIKLLLNLIRILKQHDFNQINYSTNFYTETRRYLIQKISKELVIIEFLILIVHLTKIDKNFIQCGSNSLNLLVEMKVDLTKQCFSNIKIKNTSIIGANFAKCNLSGSELENVNINGINLNCAQLFNCKWKNLKIYEVHQFNGHNDTVCSVSFSPDGTKLASGSDDNSICIWDVKTGKLKAKLEGHTHWVQSVCFSPDGTTLASGSQDQSIRLWDIETGEQKAKLDGHTHYVYSICFSPDSTTLASGSFDNTIRLWDVKTGQQKAILKGHSSCINSVCISPDGTTLVSGSIDKSIRLWEFKTGKQKAILQCNSQVNSVSISPDGTTLASGSADNSIFFWDVKTGQQKSKLNGHTNQVRSVCFSPDGTSLSSGSDDKSIRLWDVKTGQQIVKLDGHIHQVKTVCFSLDGTKLASGSTDNSIRLWDVMTGQQKFQLEGHDGIVCSVCFSPDGTILASGSDDQSIRLWDVKTGQQKASFDGHTHNVRSVCFSPDGTTLASGSRDKSIRLWDVKTGQQKAKLDGHINWVQSVCYSPDGTTLASCCVDNSIRLWDVNTRETKVRLEGHQGYLNLVSFSPDGTILASCSSFDSIGLWDVGTGQQIAKLGGHTYCAQSLCFSPDGTTLAYGSEDKSIRLMDVKTGQQQFKLDGHSDYVRSVCFSPDGTTLASGSDDNSIRIWDVKTGQQKANLMGHLNAVNTISFSPDGTTLASGSDDNSIRIWDVKTVKEVETTDIIQKEILNNLLPEGSVYNLNLASSHLTILLISKQPIFQASRALILKGQFENQSSIDLKTLFKQKGSCILESQQQQIQKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PR54</accession>
    <name>A0A8S1PR54_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1280037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PR54"/>
    <dbReference type="OMA" id="MENDRQS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="95"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="164"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="517"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8105645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="605" mass="72111" checksum="A634B2A800A0AFC9" modified="2022-10-12" version="1">MLSYQNVAQNEYQKEKDIPSPSPLSRPKDNNTNQQPTPVPQPFNTLLPANLLHPQTMENDRQSIKSNLSQKDQSFKSNYNINQINPQEQMLLEKQQFLMEEGEDLKSQVRKLQNENQQLKKVIDQLQPELQSFKNQKSIEQDLKAKFDYQEEMIVKLKSEVRRKQEALQENQYKLAELDKLQKKLEDYNLLSSKSNELNKQIKDLQDQVQDQKRQLVYLNSAEHENSNLKEYIETYKQQLREKDIKIEQHREQQEQLYLDNKKMKTTLDMLNFELKKQQENSKDQLHRATLQITELQGQVSSYQKTIANQAHQIELQKQQVKQLEIKEKELYSTKNQVLKLEESIQNLNQQNLLQQKEIEKLAMTLKNGKQEYLNQQQKLVNAEQEIKKQQDQIQQLQKYKDQIIHQCQFLEQELKKKQGNLDQSINQAQHKQIEMKTVLDQQQNQLQIMEGELQKRDAIIFTLDQQNQTLKSQLEQYSQADSLNKNELNSNFAKMKEMHNDLILYEKQVDSISLENHQIKLQNQMLQDRNNQLEFEVTKLREQLITQDNRIRNLENELQSQSFNQKLNTIRMPYTSDQLFPNQQQFLQSNTPKGSFNNQFNFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PT45</accession>
    <name>A0A8S1PT45_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1290128</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="AYRIRQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051640">
        <property type="entry name" value="GRB10-interact_GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445:SF36">
        <property type="entry name" value="FI03272P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445">
        <property type="entry name" value="GRB10 INTERACTING GYF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="1034"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="2">
        <location>
            <begin position="1482"/>
            <end position="1665"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1028"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8106154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1665" mass="198497" checksum="BDC9F582633F93C0" modified="2022-10-12" version="1">MYTNTAWMLDGALCWVSIDEEYSRGHIQKLLNQRSVARVILADNKTIDVPVNQILRASNSKAIDLTELPHISEPEVLFKIKNIIYLDRQILLQNVNTSLADFVQYKKYIDERLIVSSIPPHIFSTLFYAYQNLDIGKNSQIIVTGNKGYGKTQIINDMINFLSKDTLMYYNLIKSFITDNNGHTQGVINYRFIYKQRRLIGLLFQSYYVNFAEYQNAQFFTQKWEELQNIEILKLIQNEKIENAKQFQNMFEKIILIINGLVQLNDNIQMISILEYPGKSDQLSEYISEKIEQIYIQSVFKNCQNQLIQDQLHELNKQISFEDNLSILEELENKIQIPSNIGKLNNIMTKDTQMLFIRVIQNKQDLGQFRIFDSIKFFKNSYPYRLTFNDFYQRYHHLDIFNKKISLQKHINSNQDMKALVTEICKRLTQSKGLLFGQSQVYFKKEAIENLNKLLCNHNLKLSVYAKIIQRAYRIRQFRKVILACVKKIAKTKSIEQQVISESELAYIENEIRKQSQSQLKEEKEEEIIIEISDDESYILLNENQLEEYKKIKKEHIKKKQHSLINGQIYEEVSDVAYSLLTQEQQIQRKDNINKKKIEQQLKELEVIQNEASDPESFQLLTQEQKFQYLIMKKQNQTQVAYRLLDQNQQLKIKQQRRLNKIKDFKQKYYDQLEPFLSDLEAFKQLTIGQQNEYKKKKLQNQKNVALSLLSEQQQQNRRQYLVQQRPRELCKKRFLVDDPLSDQEAFLLLEPIQKDKYKQEKAKFKPLKLLQSMARFSVDVEVSDLESFKLLDNNKDVYIKYKQKLRLCEDISDIEAYLSIDNVNEYIRLKKEGQSGIAKQMANLKQYEELYQLRLQAQQRLDELGLVRYDLLDSIAFNLLSQNQWLQRKQQLNDYQQRKKRMNDIGNFCFDLDSLAYMNLTIEKKNERKQKEKMQILLKNIINPILFENHLHPNSSVAFRLLQNKDIVYRKISLAYILLKDKTFRDQQLKELQVYDWEEVNSLQQKQALLQEKINIYEQEKENLKKEEFSDYEAYKLLDEDQLLDYQQNAYSFLDDEQKQVHRRIIKAKLISNQFHSDLDSYEMLSDDSKEKYKQMKMGDVSDYCAYEVLEDKQEYWKEKQRKKELLEQIESQGFKIYFGATPQVMIKSEYQQFIKQKLTIQQKIKDEEMSFLKQLQQEQIVQQKKSEIKKTYIIQKLISNPKSQPYIIYDNCQLNKRILDIRSAPDKNLSFFLDKEFISKMEKHKFYDYCQQVLNHEGKQVQKIMKCQSENLKHPLTKLQNETTALNVFRSITKFAHMKRSRQTIKQHLEKILCALIGNDAQIELPPAPKSLIKAHTIDIDALKKTIQLETAGRRFSNQEDYECNQENREEILLQIFKQIEGNKKEHLHHLLRLCIVITHCIPYYHPIPYIQYLVDKIENPENKLFKDPEIYKFCKQLIKNLALNTNEQVFKNEIKIPLCPRKYLPKADEIQLMFECKQSIVKLFIHSDTPIWVEVDHHYSVKSVIRSACNYIGQINNWNHFGLIVVHSKFDTIYNTSWLDESANFFDILNKLEFHEEINNNVYKLNKRFEIYLTIKEFFQFNKSDQDILDLVFYQMVFDVKRNKIQLKAEDLQILSELEMYIRYGDNINNQKYNLSDKNMAKLEYLKILRLADSNSIPDIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXA0</accession>
    <name>A0A8S1PXA0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1330098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXA0"/>
    <dbReference type="OMA" id="TNSMIIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="106" mass="12871" checksum="3EF95F89A2C51A69" modified="2022-10-12" version="1">MKKSQFIFENKPSKQFPLDNKVWKLNENPTINEEPLFTEESEIGFQNEIKRIFNDSRCQQYTNSMIINNYTIHSISQATFNHIDSEFYQDYLTNQWDQYRYQSVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXB4</accession>
    <name>A0A8S1PXB4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1330103</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXB4"/>
    <dbReference type="OMA" id="LIDHCIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="513"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="163"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="253"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="513"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="548"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="564"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8107308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1022" mass="120461" checksum="3EF989F493321F63" modified="2022-10-12" version="1">MQALKSREQSNQQKKNNQNTKIQLIDHCIQCTNSQVNFFGGVSITQQGNPNIKIKNVTQVPWYEAQQLNQSQQKRFIDKQTCTTEDYYQNCSNVLSGSYDRQIEFENIIQYANESIAMMGEALSSRVLQQYKEKGKPEELNKLMHAIKFKKEDIDQIFKSVDLNLLKDTIDNYQQMEEQLKQHIVQVQKQTAEKVFQYCKSIFKEKFKKGRFQTADHFNRQTTLGNRDDCRCQIYVGEIVSLQNKTEQQIQLINKQTSDIKEKDRNIQSLELQIKLFENQIKQHIQDKQELKQKIDQQYNQSRRSTVFQRQSSFMVNFSQNDEPQDMLMSSPNAKMLKEERQSYSKILKTPSLQPFVLQNQVQQETINNEQFNDLVKKDKMIERLIVLTNALDKSNKDRRLSQLQYVVEQKGEKQLSDMLDLIKDIEENDLLIKEQKSFGNIQFDNDDLRSPMNASKRSYSQQIIDQSDQIQKQKFLSTKDLNSPKVLYLEQIKEIDENKHILLVPIEQTVTQGKYSKNTSQSSFNDSNQVIEDVSPQNKKQQRLRRTSSNVQQIVETKNNNNKSKRTNSIKLKSKKKVSNQNNSNEAVSNIIQTTSNPQLNSGILTDRQPVRSISQQQQEQYKYERVIENELISNKIAIHNTSLHDKPLINIFQSNHNVQYHTPNNSSRLIKVKNQNQDKLDINTINKQISNHKQNLEINNPNQQKQQESQIINPPQTQTKFQQQLNQQQFLIQEDRDKITKKQQQATTDKNQQQQQQYQYVQENQIKPDKEVTSINIIQEVKKQQQQQNQRSKLPNLEIETVDKYNKSPRKLSTQNNNNKPIDNKLKWTGIESAKFITNIDPNIQIENLNYRPQTTIDNDETKKQEGQIREYINKQKDEYEKKQKEERLLFDQVLFDLPEQQEDLIIRNAINNNQGSIISEDLNDNVLKHMYKNLKENQTQITQTINKFYQRSDEQNIDYPEFQNFINKFSNLHKRCGKDCIHVTRFYMRLGFIPLRYLNKRKAMKLAKPVVLPCFSKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYT9</accession>
    <name>A0A8S1PYT9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1370027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYT9"/>
    <dbReference type="OMA" id="CDIYCKL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="24675" checksum="5279343EED5E5D5B" modified="2022-10-12" version="1">MKRKSLLLEADIIQQIKSAFNLNQVEINELMTQAKRYDPKGTGYVSKTERHFNIFYHLDLKLLDNEKLIAKFKEELKQQDAKMLDLKQICDIYCKLKNYQQQLNDEETITQEYIDAFVALGGNPDRTGVVQKQMIFDIVQSEFELKIDLDSFLGDFQGNQLEFEDFCQLFENAGEDAKSFITSFSQAKRNNNNDFTVRFKDFEKWEKTAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZJ8</accession>
    <name>A0A8S1PZJ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1370102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZJ8"/>
    <dbReference type="OMA" id="LIEYYRY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="263" mass="31800" checksum="D5DBBB762A244CF8" modified="2022-10-12" version="1">MQLPCMFCDHKGSLPELLNMALYSRYQTDFCQLQLRSPTTIRFQDQACFEELEEYFKFYYTFPYLKDNQKQLIEYYRYHRDIPRIFIKNITSQLEAYHEKQREIRYKKIKKQLGMHVQDTTRDNMIYSFKALKGLSELQTTRQLQQLVKQIKPIVYDKSGIATLIQKQKQQMLQSPQLKRLLKIDNINTATTQATKRTYHTYHTLQDVKKKGHKKQLTLCTEYSPDDRNDFYKRWSDVLSHRQLQYHKNKIKCTLLNKKKSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZW2</accession>
    <name>A0A8S1PZW2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1360054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZW2"/>
    <dbReference type="OMA" id="NEINNQW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="56984" checksum="8C0AC4AEC6FFDDDB" modified="2022-10-12" version="1">MEIEEKYNLLIIGGGPASVKFLYNLFRTSRLPEYLNGLGIGIVEKGKCFGSGNLENKMNYTTYPATELLKLMYFHKTNQMISICKDLTDTQAYKTLIQYGEKCPPKSLAGYFLRLLGSVLLKHIYTLKKLQIFHPQREAIAIRLLADGWFQIRTQNLSADTQIKDKYRTIKARHILLCTGREQTPADIFCQEQGLIETLKQNPRHIVGSGQKAFECAELLLSGPVMYINNEQINAYTQKVYTCPLCKSLKCSCFGSLVPQKYIWHYTDVALPEYKKGELKIYFTKPPRPYFNNHQNDYITIPEQQINKKGEANYLTGLRGKSKILFLKMLATQEVRVQLSQDEPNSNQFIDGKECKCNQIEIKDTDGNLVQFQYSFGLYQLDEKGRMKTQKGSLRNLFCYGSNCITQEQLERGCQDEYGDCSIGFDEYDLFFINIKNEINNQWFDNNEFPLTAQSHVSKGGDFDSDPSVHSEVVETETFALNQLQEQFKQKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q649</accession>
    <name>A0A8S1Q649_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1460067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8111042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q649"/>
    <dbReference type="OMA" id="SFERQQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="435" mass="52410" checksum="57D093BFFAE35CB8" modified="2022-10-12" version="1">MQNKEILHKVFDFYCKQQYVQGQFASFERQQYEASIFTIGKWMHFCRDFQVKCNSARLLELFKKQAKHSKELDFDQFIQLLNVLAIEQEVDFGQYQSNLGLDNWKVCVSKMKTFQRPFQMKDQYERIKDIKYQFKIYNPEVKNDEQIKQILLQRKQQNEQLKLIERKKKAFYKLQFELKHFTKSQLLSKYPTLQELIEKLPDPSKSKIQSNSNKEQDQNSNISQQMQSAITWENLNYLPLAKDFLTDLMEQEQEDDDDQYLKEYQEEKYVENSPKKLISNNRYNSIQDYQNERELRSFSDHKQIIKSKRYEELYPIERQIDKSKIFKLKTTNNHSESNNIISLQQIDSNLIRQQILRQQKQQLQNQNYSVELPRQNLSQEYSDIPLLKEKPTTNKLNISMLKRVQQLSGLETIKEQNLLNKIISQQREKTKIAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7T3</accession>
    <name>A0A8S1Q7T3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1470062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7T3"/>
    <dbReference type="OMA" id="SIKHYEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="381" mass="44713" checksum="A781B76764F22359" modified="2022-10-12" version="1">MSLFSRFTSIFGSNQTTQASSTPINQQLQEKLAQIITSNSKLDLKQKGEVLAQYLLDRDEHIEFLFQFLETKSKDLQGVQVVHLLAALHQQFQYNEIIQDVAIKLRETKMPWVQLEKQEWYSTQTEPDLQKNQQTQKILTEFEDKAQPARIYAQLCYVYLQKLAANVDLYRAVLKLNYPYVADKEQIEAKLMFLWHYKIQNLINSGLILIQYNPNLIEIQVALYVDVWRFQKFICTEIEKIIDQYATLPNSDTLSLYEIYCESIKHYEYLNQFHQFTQNITKPPPQCQINNSLLQEFFGFVTKLKVLNQFNFKKSIKVPNKQDPIMGIPTKNPNVINIRRSSQQLNDSLSSESEVEIDLGNNKKMRSDHNRGQSTFQYVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QA83</accession>
    <name>A0A8S1QA83_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1510109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000156">
        <property type="protein sequence ID" value="CAD8112488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00047">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="44"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8112488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="27513" checksum="E6BC5D5EC2E63940" modified="2022-10-12" version="1" fragment="single">MNPQKESEQLPIDLSYKQNDNQFMKYEMAINLQEFVKSFNQRRLEKEFSYLCQITETQEHEKLFFWNEKTHHLNRYIDILPCIHTIVKSDVNEQFYINANYIRGIDNIDKQYIATQGPIHSTVNDFWHMIWTNEVGLIIMLCKLYDHNYKQCEKYWPDISNDYGPYEVKLKKSEELKCGLISNQFIIKFQQQEKLIHHYQWNGWPDLGVVNEDNFVLIDFLANQANSSX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QAX8</accession>
    <name>A0A8S1QAX8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATPase AAA-type core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1520024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QAX8"/>
    <dbReference type="OMA" id="WFIECRA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000199">
        <property type="entry name" value="IQ motif containing with AAA domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052267">
        <property type="entry name" value="N-DRC_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14690">
        <property type="entry name" value="IQ MOTIF CONTAINING WITH AAA DOMAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14690:SF0">
        <property type="entry name" value="IQ MOTIF CONTAINING WITH AAA DOMAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-type core" evidence="2">
        <location>
            <begin position="585"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="823"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="823"/>
            <end position="838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8112553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="847" mass="99790" checksum="BE3AA2773EBBF91C" modified="2022-10-12" version="1">MEALQIEMDPLIENQAKQNLKLVRPDQEWFVYYGKLYLFYVDAYKSLETCYDQLLHPQKRRLLKKMLENTMLRQIQIKQAMIFYSTQGNVIRSDFVNLHQVIFDEKRLIEDTEVSLPHYFSDEQNRKLAYRKQMLNHFLNEFHDTTQPEEEVLVDKAVLENIEQAIWFIQKNERGRQGIERVLLAKQLKKQELKKQEKQKKLQEGLEVHDSTEREEAITVIQKYYRGFKAREIVWDLREQESLFLGFTLEKPLQETDAYKTYHIQREKMKEKQQERLQEYKTALVEARDNLMNDWQDDIQNQMLAERRRWYMSEVQKAEGKLVPPKVAQFYKKDQVRLPLTEEEQEMMDLLDKEKKAAKKNKGKKKKKKKQEDEDIRVEQGPTEAIVKMQDLIEKHNQEWRNDQLNPYQKHENSLLKGEIAKGVEKQIREVVDEMIDMELINLRIALKAKKMKYPKKKKSKKKKAKVVEKAEEDDLLEEGDINFGFRSVAKKKKKKFPGDKGTAKKDPRDMLAELIESGIAKKLEPAKISDLIGNCNPLRQKQEMLQEQVADPSIYDLRQVLVQFIGMPLGSSYIKDNVKESNYVLFYGPHGSGKTHAVRALQTECDALILDLSPANIENKYQDKPDYLLSLAFKTAACFQPAIIYIDEIERIFKGKKKKKKGDQQPSSAGGMNWVKLRKYLLKYGKYFTEKQKENVTIIGCSYAPWNANKKQLKSFFKRRVYFPCPNYATRIMIFQKFFEERKIQLHPNFPISSLAHATEGFTAGNIKEALRKILTERRLILSTEIPYRISEFVDPLAVQQSTFLQQYQEFRKLHDDLSGNKARIDKLKKPAEDEKGKKKPGKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QD81</accession>
    <name>A0A8S1QD81_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1540026</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QD81"/>
    <dbReference type="OMA" id="FWRERSL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000054">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1 2 3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="4">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8113084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="243" mass="27067" checksum="0755239F1DAE89C5" modified="2022-10-12" version="1">MFQNKTDYDRGVNTFSPEGRLFQVEYALQAIKLGASALAIKVNEGVVLAGERKLNSTLLEPKSIEKIYEIDTHVACTASGFIPDARTLVEHARVESQNHKFNYGESINVRALTQIVCDLALDFGESDSKNKTRMSRPYGVALLIAGVSDQGPQIYQTDPTGTMIEYQAKGIGAADEGIQSILKEQYKQDLTLEQAERLAILCLKNVMEEKINNKNVELAVITTVEKRFTQRTPEQIQNLIDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDN8</accession>
    <name>A0A8S1QDN8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1570017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000162">
        <property type="protein sequence ID" value="CAD8113842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDN8"/>
    <dbReference type="OMA" id="ADNCENH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051942">
        <property type="entry name" value="DENN_domain_containing_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288:SF0">
        <property type="entry name" value="UDENN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="2">
        <location>
            <begin position="276"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8113842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="776" mass="91337" checksum="E06672D83E787AEA" modified="2022-10-12" version="1">MLPSHPSPVDKDKKIKIMDELIKTNQKHIDHLKQTITQLKRQLQFEQKRNQDLDKDNTMLKDQIKKLLSKPPLHTQSTQTSNDIHRRDSRQLSTKEDQQFNSASKNIQKSDNDQVNQVNQKQQILNQISPTKQEIKKIQKLDLKIDINSALQRQQSGPISSVKKSQTYLHGLTNVKLIDAIKERIPEKSNRIGQITGDRSSQIRRSLMFNFQNNKSTIIDCELTEEQEIQKSRNSDGKQQQLIESIEEYRVETMEVSALQQSAYKLYEKFYIMGCEKKEFLEFDNDPNIKEGILPANILFKSDSIDTPVYEEIINGFVYPFGNQVEKIKVNDSFRKLKEIMYSGNKYELLDKFSLFTIKNQENNDNLSEHTNQNLLNQANPEKLLYGICLSVYDFIETTPDHIKLTFENRKKRIFWKYKKTYCFLTYFPFYELFQDLLISIINLIKINRTDRYLKKYSEEYEVLKDVDGQQIILEFQDELTKFLNLIQVIKPMLGHQQLELQTLAGTLKYRIPNQLQLDIELRLWSANVTLQILNYKEILNIFLAMISEISIIFVCEKSNILTSIIHFFHHIVRPLEWTQGIIYNVPEQLLTMIQSPVPIIIGVNLTESDFNLMSLADNCENHLFVFLDRDKESKFLTKPQNVLKDISTPSFGGLLQQIETILKNQNNIRNPSPSIRQVSNKNDDKKVIKFYFDDQDQNKSKKVLETFKKIIEDYIISKLPSQNENRIKVGQDLNYQFIEQYLIQSSDPKDKKFIQNLFKTQHFQYFIQQHYCDQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QE61</accession>
    <name>A0A8S1QE61_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1560040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QE61"/>
    <dbReference type="OMA" id="AVMMTYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="46616" checksum="610D54B20978B40B" modified="2022-10-12" version="1">MQYSSDPHYFEMSNYPNNVYQIDPMMNPIVPQANLPTQINSPKGSDPDSQISTRKSFLFQYYVKQIIQLIGITLIYGINQIGSFSSIYLSEVDREWWGSSYTYEKFLKYYSYDATRQDYLNWMEERRREFMDYEFNWVYYFILALTVFFTLFVAFARNYRCLAQKQSIGLLIQTVLIGFVVGGISSGYRQNWQHQGTVFYILFSLIGLIVNAIFNLVLIKINKIQIYGKLSKIFTYFFFGFNFLIAFREYYRVEGLPILEFVVLYSFFYFDHLNKSLLIQPSELPSTINILDLLSNIQTELNSNQFKPNQNFILINKKILLIVSIGFGLVLLELFGYLISTFVFIIITIISVLTIISFVVPTQVAQQTLEIEDASIAVMMTYLDMLCPIQNIIRHRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJZ3</accession>
    <name>A0A8S1QJZ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein kinase PLK</fullName>
            <ecNumber evidence="8">2.7.11.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Polo-like kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1620057</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.21" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8114997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJZ3"/>
    <dbReference type="OMA" id="KCLANII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13118">
        <property type="entry name" value="POLO_box_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13117">
        <property type="entry name" value="POLO_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14099">
        <property type="entry name" value="STKc_PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001669">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.30:FF:000013">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.30:FF:000016">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000091">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033701">
        <property type="entry name" value="POLO_box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033695">
        <property type="entry name" value="POLO_box_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00659">
        <property type="entry name" value="POLO_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50078">
        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="32"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="11">
        <location>
            <begin position="438"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="11">
        <location>
            <begin position="541"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="335"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="621"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="337"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="626"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8114997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="643" mass="72915" checksum="8E1A06C6309377B4" modified="2022-10-12" version="1">MDEKNTAESSEQVVIFEEKITKVNGEVTIKRYQRGKFLGKGGFAKCYEATNLETKKVQAAKIIAKSSLTKNRARQKLISEIKIHKSLQNTNIVQFEHVFEDHENVYILLELCSNQTLNELIKRRKRLTEIEVQCYVAQIINSLKYLHASNVIHRDLKLGNLFLNKSMELKLGDFGLATKLEFDGEKKRTICGTPNYIAPEVLDGKVGHSFEVDVWSLGVIIYAMLIGKPPFETPDVKTTYRKIRLNSYTFPEHVLISDAAKNIITRILNLDPVKRPTLDEIMSHPFMNTGGSIPKTLPLSTLACPPSASYNKQFQPSTNSSSLKMSVNAMPQRLTETTPNNQKNQQKPGNGSSDRFPLQKPSSSGNILEDNFGSSGLNNAQNVGYGGSQRPQSQKPNDIRNSQSQKTLTTHFGGTGMQGVHSVNNLGQRQTQQKQEIYVKKWVDYSSKYGLGYLLSNGATGVFFNDSTKIILDTKSLTFEYMERKGTDKQDICESHNLNDNHPRELQKKVFLLEHFRNYLEGEQNNQDMAVEEYDNKQVVYVKKWMKTRHAIMFRLSNKIVQVNFTDKTEIILSSEHKMVTYVNKAGERSHYPLATALDSQNTEMAKRLKYTKEILTHMLNGGHTGEQQPNQMTGTNWQNQGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QMA0</accession>
    <name>A0A8S1QMA0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1790008</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000188">
        <property type="protein sequence ID" value="CAD8116739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="3">
        <location>
            <begin position="198"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8116739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="455" mass="54108" checksum="3F4B7ECA5D9C7908" modified="2022-10-12" version="1">MGSKEVELKYIRESGQLYFQKIKILQRIFLISSFNRDDKLNLTTTEFINWANSTFKLEQDMCLECYPISYQALKNKLKMILVNNQAARLIDSPTGLGYMLKYDQTDELKLLFKFLIQNKECIIYISKYFEQKGQLINNSFENQEQIEQQKNEAELYFTNILLLMEKAQDIFLNQLEKEPDVQRLYKEALMYLVNKHEKSPKLLAIYIDIIIRREKGGMEIESDKRLTQIVSLFKLLYQRDIFFNHYYKLLSKRLLNQQIQNLYLEKQLIQKFKGEVGTNVLTKLSTMIQDIELSNRFNIDQSISKNMQFDLNVVLLTDGCWPITNIQDNIIRPLKIQNILESKQRKNTRMVLQFGTGRIDIQNVNRKILLECEYMQMIAFLQFNKADSFSIKQILDMTNIEKQILENSLIPFVCMKIITREKQDIEDFSDENEVLKLNMSFTKRXLIITNIRWRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNE9</accession>
    <name>A0A8S1QNE9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1700012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000179">
        <property type="protein sequence ID" value="CAD8116195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNE9"/>
    <dbReference type="OMA" id="YSFQINK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="514" mass="61045" checksum="A2436B6F3CA2F19B" modified="2022-10-12" version="1">MKRSRFDFQSAAERGLVKGDQIGQKLINQNEPDYFNLPSFILPNQSNALNLSVQLTRRMNSDNHTPELQQKALNESQMTKLLLPQIDNNQVNFQHSTQLYGLNDENLGFYHNFLHEQSFQQNNGKKQILRQSTYTQNQFYLNHSPRKRQYSFQINKNFKSNLDITNEKEDSNEIDDSILKNEKNNILKEKDIQQDEDEDVSHVSNPMITQTTLNFKTKVQFQYKKIKICVIIVRAVFRMQILIRPLKQKWIQNQQLFCQLINLFKFKDKLVQNKIKQWTQISLTKVLSVLRFQTLKQWNFIEGNIEGFQKDMAITNFLNLCSHLISNLEITTQQNSFLPELGYQSYLEQFVEYRQTYNIFVTKRTNYLQNKYSKITPIEKAMIATECVIMNNFIPNLVLLTENKKFFNADDQNIQFLIRGFITILQQLFIMTFSDIPQVQTLNSQFKYQQMQIGRSKYGMILVPIQTDMEEFYKGTFKYDQLKLIFERRGLFSNLVKQFKKVVQNIYQHKIQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNR6</accession>
    <name>A0A8S1QNR6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1700013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000179">
        <property type="protein sequence ID" value="CAD8116197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNR6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="337"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="338"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="386"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="386"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8116197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="771" mass="90639" checksum="386BEB19696A7999" modified="2022-10-12" version="1">MQKLSNEYNPNEFVIEAQSSKAPSSQEFEFETQSFTSKEDLINNQNFKPFLQERDSTGKLVGKDKKLHFLKGQQIVLYTSGSKRLQFSTTSILGLNSRAVTADDRRMAKREPLKNFKLNLQADQGFIKQFREITKHIKPSSQMNSGFLGPHRDYYYKTTKTPGPGVYQSLYITATNPIEFPKSPQISKPAQPVCSKDFYDFNMVQKNISGPSFVKTQSRDSAYQRGIFAQIEIEKRQTLKQKPEEYDEQRIDREIDYLLQQQQKYGKLSQPNLAWPVPLQKIELPEKVHGQWTKMLTLWIQEVNRWKINKEYKCFMIILCRNKKLGIKKYYDYQQINEQEDLAILHLVKQYGIKKWTTVAEKMKEIYGLFGRSGKQCRERYHNHLDPTINKDPWSENEEKIIFLAHREHGNKWAEIAKLLPGRTDNAIKNHFYSTLRRSLRRINKMIGDKNKKCTQQIKDIKPGVLSKIFILAEKNPSELKDDHMKKLCQACKGLQDSILEFAQSKQKSLIIQFNEEKFKQLIEKIMDFNGLYTRQRENKLKSRKLNLKKRKNIIEDDDEEDDLYSSFYKIDDNSIQIPIKRSIRQSSRKKIKFNDEDDLDIIIRTKQGPVFKIIYDRFQFQTDENESSQNYYQESLNSNQSEQKQISYQINPSKQISQKSISKDNQNEINLKNNINNNSNFKPIILIKPVINNQEFDQYLEKLKQGINANSQKYLQGEDLNLDINIDFADASKDFTKSLSSKFEYTQNAFKKYKKDSDLDNFLVTPNNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QTW5</accession>
    <name>A0A8S1QTW5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2230001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000232">
        <property type="protein sequence ID" value="CAD8118077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QTW5"/>
    <dbReference type="OMA" id="GHDICIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="1">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8118077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="198" mass="23397" checksum="4C9DA64FFB29EF04" modified="2022-10-12" version="1">MIYQSTRDGLNNQQYWNKVNGKGNLLMVFKSKSDYIFGAYSPCKWESYGGKYVEDNTLSSFIFSQTHDQVYPLRQDQKSNAIHCNSNSYGPLFGGGFDIRIDSNFTDGYCKLGYSYQFSQYKNQSEDPYLFGQNKPEIKESFDQDTINIIVVQQNDYDKKLDAIRRKKISLCFYYQIDQYIRYYQVLFLNHQNEKNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JML7</accession>
    <name>A0A8S1JML7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JML7"/>
    <dbReference type="OMA" id="NYLWCSS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="187" mass="22856" checksum="6131EC1B261F67D6" modified="2022-10-12" version="1">MFFDQNSYPIYKQCIDGRHFLVYPIYFIQKVNRQESSVNQELKLEEPEHKIETKIENKEHKNYDPMDFNTYMFPGQSKNYYKNIGNQFASFIINYFDKTEILKDPKISKFLKIKKQKYTKMHFQQLMKSKKARQLIKIYFGNYLWCSSILKRMKSDVEFYLSLNQQIFRRKPKQKIKTEFNKFPGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMR0</accession>
    <name>A0A8S1JMR0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0040271</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMR0"/>
    <dbReference type="OMA" id="NQKNTRD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001744">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="40"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="337"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="399"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="356"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="428"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8043051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="630" mass="73031" checksum="1654322CB787A591" modified="2022-10-12" version="1">MIRRPRFLSPQERVKSPPPHVNLQFPHKHSLMMQTGPYVFDRSQIIGKGQYSVVYKGYEQNKPSQIVAVKVITIQSNNPIQMYMIENEINTLQLLDSPHILKLLYHHQNNKTVFLVTEFCNKGDLLQKLQRSLISEEEALQYTLQIAKALQTIHSHNIMHGDIKSANILLSDRCVLADFGFAQKIDQAQQTFIIGTPLYMAPEVLFDNEINCQADMWSLGCVLYEMVYGQSPFYSENNDILKNTLTKFKEHGKLIFPNTGVSKKIQALILYLIRIDPSQRLTASQTVSLLEPGNVNEQIKLNENQIKIKQELLNLDEKEAPNLMLQQQQQQANINENNMNSNSQSNQTSIQDIQSTPDNQKNTGGHNSQDEHRRSTKNSNSSAKRKKLLQQLNNLNSRNINSQINNTPSQQSQPQNTQNSDVKKIPQKNIQYPQQINPNTPINLSNNNNNNNNNNNNNNNNNNNNNNNNNNSNNKTISLLDLVNYFYKVYQLIINGKEIKLAFIYLKLVNIYYLAYLEEQSKQQKKVIFVSCQNDYFELEREIKNKYPYWLKEEEFQKIFHSKFTLTQDFLKYSLNYLNKAAVDQQQDEKITEKIKKTLQIIQILVSNERSSTLQLDQQIIRLHRLNSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN33</accession>
    <name>A0A8S1JN33_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JN33"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="600" mass="71928" checksum="14EADF91EAA04416" modified="2022-10-12" version="1">MGNNQSQNIKSKLKKKDDLIFLFNNNKKQNVFSKLSLKTLSLLTSFLSIQEYYNILLTSKETYQLFIQTSGCFQIECHRLLNINDNLMISQNWKKIIQSLLFIPIKALPFRESIKSVRQELKQIPILLEHKVAYSGFQKAILYFQNNSTFDNQVLQYKEYQESKQFAIQNQDLVLKVRQGLDVYIQLPSILMQVYNLHDYIKIYSLYLEIKFCKCNLQQFIQLWNHYKSWISVLEQDTNNIIAQFNYIINAAFPLQRLPIYTVRQFMVSQWLKKSDRGEIIVMLREEFRLKMIESRNKNIKFQELRDYVQYLIEISTNQINIHQYGYYNFEYCQEINTLVHLAVDLSPLLTINYQSDESMLIYIFGQYIYNYQIFELLFKFRIEQFKQQIKIHQQASQKLNQILVQKVEDQQSLHNNNLFDNQIQEFNYHLSMQYDVELKIRSLFKQISYSNDDICTISDNKQTESLQASKISLASTNCSSFFQSFIINDELYHYIKFNQQELFNQIQLFYQRGIAISYYQQEIEPENNNIYDIPELGYYYELTFTQLMQLFSNKNQRSNSIQYSKRQISISIFDQQLNQHKINTKQLSQTEQLLINILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRZ9</accession>
    <name>A0A8S1JRZ9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cuticle protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRZ9"/>
    <dbReference type="OMA" id="NHAAPWA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="22104" checksum="21EFCCE7C087B228" modified="2022-10-12" version="1">MYSYAPLGVSYASPLATSIARPLPVGYAAPVSYAAPVSYAAPVTYAPPVSYAAPVSYAAPQYSSIRGESRVEYVPYQKPVVELEEEVRTVQVPRQKWVTDYYPVEYQKEYVPQVSYEKQIDYVPVEKNVPRVDYLEYEREVRRAPPAPVSYASPLSYSYVAPVAPVRTSVVAPTYGYGYAPSYSYAAPTFGYGSVYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSB1</accession>
    <name>A0A8S1JSB1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0040299</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSB1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="244" mass="28756" checksum="A68F8DF52EC7744B" modified="2022-10-12" version="1">MKQSEQVLLASQQPGLIDTSTKRLISKSSSQFQMPSFQQQPVVTRRIHRKLVPMDGEAEETVVQPIQPPPQPIVHPVQQMIWPVAVQFNEDDALAKIREEKMLALLERQSEMLTNLLQKYKRLEEDQTNKIQRRIKELERENEEIWQRRRQNYLIYNVSQSVDQKLKEFNEFQMRPFLDQGQPVLLKPIHQDDRLFFDKKKKKPKPKKIVEVDDGLVQDPDTGELWDPKTGEVVQQPPSLQQPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTA4</accession>
    <name>A0A8S1JTA4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTA4"/>
    <dbReference type="OMA" id="FSPCINA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="243" mass="28974" checksum="3CBF832CF1466CEA" modified="2022-10-12" version="1">MKTLVLQYPKQKTMYNPSKPAVKIDTNTQFLQTHDLKASNQKSLNVVDRLYQQEEKKRNKIQKLKQEKERSQTPTKISFISKKSQQILENKNLKPFLERQNELIQEKKMRVELQKILATQEQEEVEQYKVLPSRSHSQFIKDSEDWVQYKEFKKQQLADEIQNQNEQSFRPLINPKSAEIADKKMKKSGLHLIDQADRLAMPIRQSFSFEKENYSFSPCINAKSRQLANKYKYQRSQSPWNQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVP0</accession>
    <name>A0A8S1JVP0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0080114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVP0"/>
    <dbReference type="OMA" id="PIALHFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="36711" checksum="76EBCC308BAAF818" modified="2022-10-12" version="1">MEAYSKFDFDRNENFQQYLLNVYPLPPNLDKIKRKWYKKYIDPEFDINQEFQNPSQNTQSQENQKPQKQENYQQPQQSQQQQQYNQNRQQLSSIPICTIIEGMLKVLYFPALLFLLSPQLQKFLNFGSVFICLLGIYRLQGLPKRNHIQEYIHKIIQLEFTSNIFYIFSLFTIDSFAFQLPIALHFMVGAAEFWKQINHEDGISLKIANYIKNNRTEIILTKQKIEIYLFLYSVFGIFIKKTSFIHAMIIFQNVLLKTKFNINMINAQAYIRVWYADKLSENRILPEGLRKILSLIWKGYEKLLHLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXI0</accession>
    <name>A0A8S1JXI0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXI0"/>
    <dbReference type="OMA" id="KNEHDAD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="418"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="478" mass="55789" checksum="DC3609C537E269C0" modified="2022-10-12" version="1">MQRVAPITIKEPFVKVVIPKQSSQPNISSEQNNFFHIAPKFQQQRLTIKQTVPTSKSIEEKRYKQHKNNISENTNNRTNSNSKSRVIATIQNEDDSLQTSENGLKLRKIVGDSHLFSTNVLQQLSKQNLNTHLTNDSQSPGQRKPIQINQELHNLNTQIQSIIIKKKYHTKWEIYKELLLKQCNLLNVYKHQFIQSTSFNDISVRIINNLFEMMNNISIEILCDQESKFNEIIEKINQEKELCLNNCKLIEKERDILIQTLNQLKSPKQSTNTNQIDSMDTGQLQQMQNAMQIKLQEMNEKEAKLIKLVLAIKRSGIDIEKIYNEEVLNDDSAVEPNDKYHGVKHYYFEKNEHDADNSVVNDSDESSFCFLNRLENDSILDSLRQYQYKCNNNLDKKTNNVKLKLDLSNLQQKFQSTQIKQQNNKSQSSQILQNKQSKLKISENQGSTGFHQEFMHKLNEFSESWRIQAIKDEKRTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JY79</accession>
    <name>A0A8S1JY79_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JY79"/>
    <dbReference type="OMA" id="GPKKQIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="420" mass="49523" checksum="6440D0843587C31C" modified="2022-10-12" version="1">MEFNQDAFNLLFKQLCSLSLLSQKTTNELNDIYHHLANQEASLQNQFFLHQTINESEQKLINRFIARISEAYQLMLAKRQIARSKHSKFLLSINHCQIKKFDSKQLLMTSLISAVYQECRLQIELILNKQQDAQNQRIHSQDSNQQGEQIQNQFYSMEVTNKKNIQNEWSINIKDNKKLNYFYTKIFPKQYEVIESADKIQEEQILYFQSRQQNKDRIFKQLLSQNEHIQQSLNWSLDIDLHNYNRQIETKLQKIRLIQLTTKQQLSNQSSPARPSQLSNKSIKQNNPISPLKNINNYPKMPQIIIKQRSISQNVPQSEFRFKSSIVQSNQYQGNSQFTSQNQKQIQNESEKTILFAKLIEKYKQNQSQLNKIRVELGPKKQICSVLKSKQLIDLIPKHSSKLKPRATKNLMQQVHSIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K050</accession>
    <name>A0A8S1K050_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120227</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K050"/>
    <dbReference type="OMA" id="HMEHNNN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="634" mass="73187" checksum="23A9B067C7834863" modified="2022-10-12" version="1">MQEDLLQPSAFQVTLRCKDHKQGLIVKGCVEKNCKRKKILLCQQCLNEDAHMEHNNNVIGIKQFIAEFSQSIYQATQQNKTSLEQFLNNDENLKVYKYIQDNKHHLKKVKDNIDTQKTLVAQDMTFIVEQFQLYLQQIKENVFRKLDLVYSEYESLFEQTRKQFNSKFFAAANYFLSTPMALTCKLGQQGDNSEEYLRKLKLIDNTSLDSLQDLFTRLLLANESPKIFESDQQRLYSTLQEPLQNYFLGWCSNQDRLPQPPQLVQQYSNNSNNFQQNQVLNSPQQRPILRENYQLSDSIVISKSGIYCKFSRALDLSKKEWPHIKLNRKFTLDFTIGAVIGLSKTTFVMIGQNDLKMRVFDTEKKSLLAFDGHTQQVNWIEPLSANTFITGADDGQVYYWIFQGQTAKIQDQYTFSNKPILAGLDLQNNSFAFVDSDGRLGYKNITTKLELFLTVGNSRLQGRLRKYKMALIKPGELFCVYVAGEQGATIIQVNEKEKQLRKVNANTLPCSQTIIPLSINEMLIASSNGVKICNTNRDDILQGNDVDVVLMEFTLSQIMYAICIPVEIGQCMQYRIQKKDNGLYDIQQKDLTFDGITYLSSKLKPRVQLIKNQDKVIMVIVDQNKINFFDITMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1G6</accession>
    <name>A0A8S1K1G6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Homeodomain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0120377</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1G6"/>
    <dbReference type="OMA" id="CDNNELQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF69">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="31"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="31"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="2">
        <location>
            <begin position="86"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="88"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="88"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="134"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="141"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8048232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="47648" checksum="52E6386625637077" modified="2022-10-12" version="1">MDHAQSFEQDNKPTVNEGHLNICDNNELQKNGSQTRRAWSLKEDNQLKQAIKIHSTNWLLVAQQLQNRNPSQCAQRWKRIKPYNYNIRKPWTNKEDQLLLKLVQAHKKNWVQIAKCIPNRTSKQVRERFVNKLNPEINQEPFTKEEDMIIVEGYKSFGSKWCKISKLLQGRPENIIKNRYYSYIRKRYFNIENPYYVIPQPNNELQKFNQEENQNSKQKSIVKRKIEKSKIRKRFTKLVNIEQDEQKIQNTNKDNEQQLDIEKVRLQVTTIEENKQMNNKLNFSQLQEQDDLGVLQNEQIIKEELSNNQSQVKQEADQDQVGKLDQLQESLNLSQSCYAPYPLIYNTVQPYKMMYFLTPQIPMQFIPQYYINGPFVQYGVQSQETLLSKSQEIQQKSQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3M1</accession>
    <name>A0A8S1K3M1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0140004</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3M1"/>
    <dbReference type="OMA" id="TIWEGQD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="236"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8049467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="471" mass="55699" checksum="57B8E452F47E17B4" modified="2022-10-12" version="1">MAKYFDNIWENIFVICFGVLPLLYNFASLAWKKQNKNYRAARLQFNGKRKIFILNQCSIFTKIAILSMFTLLSILQIIVNIQFHSDYVLITIIEFCQIINYLLAGLQIFEDADKYGQTTIATQINLILQFLCFCGFAIFLFIWQIYNKDKYVIKHEDISCWLMFILRGVGLLLFSVIVFFMPQDIKLLQTVVSEYKYKLQMLGDEEEPQIIRQQLITNESNFTFCQPGDQLQQQITDVEVNSYQDNKDINTQRKYIIYEITFKYQGKHKIVFRSFSDFLTFHRDITLTYPKFDFPQFPQMTKYLNSQDIDNRRKQLDLFLKAIANQFGNNDFFLDFLNATGKSLQYKAKIPLPQQQQQKPKKIATQQQFSKAKSIQSLIPETIWEGQDDEDEESIEADTRKTKKASTYVKMHFGEERQYQRFNSVSNLDEDQRNFNFEKPSNDQIGYDQIQDNSPFEEEEKNSLQNVMIMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4G1</accession>
    <name>A0A8S1K4G1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF726 domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0120080</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4G1"/>
    <dbReference type="OMA" id="HWNCSET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007941">
        <property type="entry name" value="DUF726"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920:SF3">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4 TMCO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05277">
        <property type="entry name" value="DUF726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8047770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="852" mass="100275" checksum="E979E7D033073227" modified="2022-10-12" version="1">METAQNVIPKNLTTYFNTVIGALNIFQTMSNKFYPEYMDVNNAKNFQIDDPTVYSSIYQGNPQLENNKRKSRNLKIEHYKLVNQFLDKYNFDKQFNSEIIGQHFFKEAIGCCYIDLQEIINDKTGLFYQQTGILDKTKWLNQAIDEVSLILLEYFKGQFKKEQIFDEYIVSFAEIQNFVYKKVIKRAKSTQKDTYIKLKQKIKQYFKELHDTDIQFETICNQIKLKIIDLLDAKTLIEKQYKIQMDSFQDYISQNYGFNLTNAIIYKYCQKLKRNYVSKAQESFTPYLNFDFQDKYKDYLALIEFYLIQYFHNKQFVEGITKQDLNSNLQYLIYSIEWIQSSVEADEALILLDSLSNYNSNISQSKSQIFWNQQFSVNVLANFKNHNKQRCEKAVQWWFDQVDEQTNIQVICQIYLILAGIKFQDDKLIFGDYEPSKRNAFSLILRQLNNGKFQYLNNSLMKLCEQVTHKIQFQIINEIENRLKNNTQDQLDNVMQLPFQIELASIQVFVFLYGRGQNQLQINLEFYKKYECLKMIVQPENANNLTAIKDQQFSQFQKQFEEIKLKLIENQKKKMQSNELPSSLTFPSQIYFQLLSNQDHNSNVITLCISGFLSGDEDKQSQWGELLHSCSGTVIGLHWNCSETKEFFTTVTQSLAQNLIPQILSKVNFFGVAMSAVQLITQNPYEKAHKEAERVGKYLAYLISDYQLFGNRQINIICHSLGSVIVLECIKELDKIFEKQKKQFINEVMIMGGVADVYKLQKRRWNAVAGKIYNIYSKKDLILNYVLTVAKLFDSPCGLKPIYLGYKQVINCDFTEIVNGHSDYWNKMMKLLMHSDFNNDFKFMTSSIKEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7N5</accession>
    <name>A0A8S1K7N5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0170257</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7N5"/>
    <dbReference type="OMA" id="NIKFWFE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002257">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="115"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8050837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="500" mass="58673" checksum="B4FDC13451662E31" modified="2022-10-12" version="1">MSDSSSYFDIQGKGIWEQCQNKLNEEDILYKGIFLFHNKSTQFCLTQYELFRFKKNGELKKIELLTTSISLLNDNTILLQKNHKQVFLKSTPQNIKFWFEQIKNKCIQNNFRLDYDIGELIGQGQFANVYKITSKKNNQNYALKVFQKVALKNLNVDNVSVAKEIQIMRQLKHPGIIKIYEVYEDKEFICIVMELMQYSMKQQKKRHSEHQCAQIMLQLFMVLQYVHDHKIIHRDIKVFQLSLIFKPDNILYKNQDTICICDFGLADFYNPLSVYQYQRCGTPGYVAPEVLRDLKYDFKVDVYSAGIILYTLLVGKQPFAAHSQNKVVQLNYHGKIDYTQVKASDLCLSFLKSVLSLNPQTRPSAYEVLHHEWFFKTLGESNYFQLLNNNGQQIQPIQTISKEIQNFQAIQSMMDEEQDESSNSKIQIEIPQPTTQYRNNNNGYYQSSIPLQQQPQQQLQIAIDNIQEAIKQIKIQQQIRELNYLTQTLHIQHKEMSGID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDD5</accession>
    <name>A0A8S1KDD5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0200244</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDD5"/>
    <dbReference type="OMA" id="IINFTHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043504">
        <property type="term" value="P:mitochondrial DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000124">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF34">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="4">
        <location>
            <begin position="545"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="299"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8053269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="724" mass="84893" checksum="E0E532ED4322B29B" modified="2022-10-12" version="1">MKSQSIYKKSNPIRSTCIKSIEANQEFYLAVIEQQMCVSICAYNEQTGEVFVTQFLDCTTFNITSSTILRFAPKILISIENNEKSMFFKQLEDELNDTLIYFLPLNVLQIKFELNQFSKFVKQLNQLQLCYAALSYTVNQHLNILINNLPVQFYNIKEVLVFNIQTTLSLDILNQLYKLFKPITNGGKRLLKANLLQPFSTIQMIHIRQQNVKEMLSKDDSTIQTLKSHMMKFKQVDTLACQLQSKSTKLQDQLQQIFKIYNFQKDIINFTHYLNDQKLNNIMDIDINGILMSDEFQNIKQQIEQYIDVTLQLNNIEEDTLFFIMLNEKSNNLKQNRLQFIQLRQEINNLFQRLMSCFCLNLKCKKLKTNDNSIKCLKSGYIFELSVNEANRNNINELDIKRIVEKENDSISFIDKNNKKYSILTEKLKDLNNKLNDCIMNILSETQLQVNNLYEQMQKFCSWIFSLNCLISQFDIALCIRDYTIQNVSNKQQMIFPQFDHNQFIIKDASYLYSVNSHDTFNKLTISFNQFNIIYDYKECLSEQLKLIGQFIILSQIGCTLPCSQFKQQLLSCIYTHFQKYTIQNLAERQSSFTAEIQSLNNLLVSELHDSLILCDSLAISSSHESNVSFSISFLEAIFKKTHFGLFGTQNSDIMQISDLIQCKCMISNNNQIDQIQQNELDQILDEEFNLDYKLKLLNKLQQTFDFPSTFCLIFERNFNKQYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDZ3</accession>
    <name>A0A8S1KDZ3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0210172</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDZ3"/>
    <dbReference type="OMA" id="INSTKHA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8053890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="265" mass="31448" checksum="B2A7755DE2D760D4" modified="2022-10-12" version="1">MKRLIQSTDFNVAIPINSTKHAINDDYYMFQKYKCSSPAILGDVLRCNTPVQSQLSPIENQQKSCFSLLSDNFDFSVKDVSRKYNNGSRQLSIQKENEEETVKIQRIIQQEQIILEEQEILNKKLQNIRLETNQKLEELDKQYLQKRSEIIKQLQFSIGQYSSNFRDSQIKEPPLKSALKKKENKRQHTQDKYQNELNDEDSQDDWNFIHQAKINLEKQRTHQNTDDERTPSKVRFVSTKDANQQKKMRKSINISDLNMFQKFRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLW0</accession>
    <name>A0A8S1KLW0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0240278</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLW0"/>
    <dbReference type="OMA" id="NCNSETR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="1034"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="339"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="242"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="339"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8056370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1144" mass="135946" checksum="59053103BA8EB085" modified="2022-10-12" version="1">MKQKNKMQLPCCKNHDFCGMKYDFHESDKSQIQIQEERALSANIDQFRQSRGISGERQINLNQMTEQALDDWYCQSQNRPMEQLLNNKKRFYVPQQYAEELKSRYRLPTTQKGIKEYLFTDQISKNEKYIIQNGESILNPKNLLFNHTKLEPKIILFGQPIQEREDKKLKRKQVGSRRRGVLLQSDENYRRLSSVDNQSRRFSIHEQPTNQNQQQRTIENHIFLQNYENITRNYTKNEEVPLSFAYKEINQLQQQIEKQEKVKQPEENQIIKCENSQQEKQMIDNNFKSMMKFHQKEQAEIRKQLNQAVQSIKQLINPDSEQEQKISNLNLIPKPFQYTQTQQSQNQITNTDKISQGHQTKKNSETRTRIHTIDDMESSLLTSDMIKYIKYSRNNEDHPINKQKFAGNLVDKINHKLVKEEKLKMCETDSIDYKPHQASIKQTRQIRSSVQQNRKISSVSRRTTINNNNLNMSLSLNKSNQSQNVTKQIIAWYNNDSQNQNESKQQDQNSNIPEIKQLFNYINQGRSENVEINEEEDLIYIQQLINPPIPNKSIMLSNKQSDISVANSKQLIQKTNQLLNCNSETRIKYKTELVNFNALLDEKYEKQQEDKYFWYNKGIKHIKDNIETALHCFKQALITNPLDSNAVQLVAIIYEKMGKIMTAKKWFQLALQFTETNQLKFRYALCLYKTGSFKASQQLLDLIIQNDNSKDRSLYVYLRGICAKRMFNLERAAEDYKIVKESSRATNYSLCIHFMLAVLFSQKRQLEIKFNPLVFCEFHKLVTSIIEQDVYIENLVKFWINQSWVREEELLIELKQTSFFKRIATSILRVFLKKMKLIVVEKDNVIFPKENEVHIIVAGNVNVYDHRVQYKRPDIIANYKQGDILGCPDKDNGLCNISDIWFLTQTRLELISIQKNYFEELWEAQQQMEGIELLTRLQQLSIFKGVSILTLYKLVYELMQKCILKKNTVLFNDGSYQENFEYIHLRKQTKATIQMTHRLIDQYKHCHFQSKLKKLVIKYKHKIDQLKYNRMHLEGFYIMLSGMCEIENPNGFNNYYIKYGDYFGETLMFNTKGFNSFGRIRAFQNNVTILKLSRFHFNKIPTLDLKIMEKNCEKRQEILDLDRIYKQRQKIKEIHNQKQHQPYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KPU7</accession>
    <name>A0A8S1KPU7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KPU7"/>
    <dbReference type="OMA" id="MNQDRVL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="79" mass="9343" checksum="15D137FD2EDCF168" modified="2022-10-12" version="1">MSFLIKLIKMNQDRVLQQETKKKTKRFDKFNYPILKGVSAKAVTFRDQIEGIPIQDIYIVERLQYQEQKKKCSCSCAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQ51</accession>
    <name>A0A8S1KQ51_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQ51"/>
    <dbReference type="OMA" id="FRQTQTY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="165" mass="19888" checksum="1D405FAB8B603D95" modified="2022-10-12" version="1">MSQSHLKCTCQSYRQTQTLREDPGLIEKLKERQMELEEKLMDFDHKKGNLERENLMLEDNIKLELQGLKENQVIQQQLTEELRDNKILTYKKQNELHILIQNVHENEAEKKQLMNEIEFLKQSLLHTNENQIQLAKKEEHIKLLLNRISELEQNITQLKREVQYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KR21</accession>
    <name>A0A8S1KR21_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0230047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KR21"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="18"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8055176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="83" mass="10041" checksum="1D55C9C0334202E9" modified="2022-10-12" version="1">MRKMIDAEQKRTDKSNKIMFMDRECQELQKAVEDLEQKIQYTKKVETEKLQKEAENHKNEIEKIRAYNNSLKDELGRLLEGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVW3</accession>
    <name>A0A8S1KVW3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0250079</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVW3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496">
        <property type="entry name" value="FERREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496:SF0">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Rieske" evidence="3">
        <location>
            <begin position="49"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="224"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8056836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="394" mass="43535" checksum="6219F7189688923F" modified="2022-10-12" version="1">MRKILSSAIAISLRRTPLVVPKRTQRLAATLVSLPLIRYGFQTLNLNDAYEVSIEDNLNEGEMREVQVGPKKEDAVLVCKVDGQIYCVSNSCPHVGAPLSAGFLVGDKVKCPFHNASFSVKDGVHEEGPMFRGLQTFPVKQENGQLVIRVEKQLLNAPRTLNMVTKGDDPTHVVIVGGGDSITCMTLLGDQYVASGGKDRNIKVYKQTYTNNVFEKLQLSMSLSDAHPTEVTSLAYGGGNILYSSSASGELKVWDFMDGTLIKQVKNYAGWCFRIINFQIEEEQTPQQKTQLQTSIPLCGSPRRKKQTQNEYECIKNCFIGTVSFDGTFKVFNGKTLLNDNPQPMITTQLKSLHDCHPFGGVLAIRDSITDKIDILSSGNKNDCNIQLYSLAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXJ8</accession>
    <name>A0A8S1KXJ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0300004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXJ8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="225" mass="26774" checksum="64D3931AA114358A" modified="2022-10-12" version="1">MIIDLVASINQQIEIQQVMMILFTQNVNKYHENTHYLQLRLQILSLYKFDSSIDDFVGQTHMLQSSSSLYINNQALAFQELSAALQYLQQAIVKTNKIKKNCMNSLVILKLIQEQQRYMKNNFNKIFTFILASQYIHNKIQMKQKKMKIQYIYQQILPKYPANLQEHYLQHQKLENNSMQLQDSKKKAVIKSFFNYHLQIYLIMPKIIIMENSIVIFLIEIKLIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYW6</accession>
    <name>A0A8S1KYW6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0270319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYW6"/>
    <dbReference type="OMA" id="HQSPDEL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035753220" evidence="1">
        <location>
            <begin position="17"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="150" mass="17704" checksum="28B6A774933F2B14" modified="2022-10-12" version="1">MFIRLLIFIRISVINQVLNFMKRKSTFFKYGKHQSPDELIKHLNQCSLNPKFIENKSTADVSKQNTVVLDKIELRKSKSRDCIQSQTINRSRLQREFLRFLLTTDGPSSEFYQLEKKKLQIKLQDTYETAQPNICLSKILGQYQELVNPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZ53</accession>
    <name>A0A8S1KZ53_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZ53"/>
    <dbReference type="OMA" id="SHNQFQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="141" mass="16155" checksum="4DED1F70789BED85" modified="2022-10-12" version="1">MGTCQSDNQYQNESQPQSEEEHIETEQMQYVVLASWKHDQQNGSNKPNHIIFGKSNTDYLQGHNQFQINQGSVQNSRIAVSSNQLNVSNIQNITSKPLNQIEQKQRFSAFSGYSQTDRTAGLVKDQDIQRRPINIAKRNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZU1</accession>
    <name>A0A8S1KZU1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0300180</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZU1"/>
    <dbReference type="OMA" id="YENQICK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05579">
        <property type="entry name" value="STKc_MAST_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000604">
        <property type="entry name" value="AGC protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000633">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000340">
        <property type="entry name" value="Serine threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="653"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="376"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="454"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="517"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="376"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="541"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="570"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8060527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1071" mass="124423" checksum="90DCBF96D9F9C849" modified="2022-10-12" version="1">MQKDEPFVTNFYIEQQIKILKPHPIAIKLTADILQNYYQLQAKESIKTLQSQLLSIYQFFQLDNFEGQQEINNKINTLLDEFQKIDGDSIKKISFRRSVPRKSLAKYYLCFFIINRSFKLLSLKLRISFDVISKIWINKQTFPNQLRWACQCIRNIQSQLNQDINRVAKNQSKLFICRICEQQVQSIDMTQHCIQCEKNAESKKRYIELNLELANLCDQAYQEKRNVQVKLAIKKMKDKNKQRQYTNTQYIFRRVQTLDDQDEEDDEKGEYQQEQNYIANIMTQIINFAEKTLNNEVEIEDTKLTLVLLNDLVSSTEYIENQEALNIINSTHKCLLERLEYHKKQEGYKNAKNQQQQQDQSEDKINKIRRAFTKSNSISFRMPRFQNSPSPPSQRISTDPIQEEDDSPTQLQMKTSQKIIPQRKGFRMNSSIFKVSDGSESPKSLKNISIPMNQSKSSGFHNGESNQSINSNNTDNKIDKLFKNTNTDIKNELDQYSPTLKKLSSFKAQLQPLNVDKKQPSLQEISPNLDALNPSIKEGENSSNLSSFESGKSNSATNNDANLQNSQKGSCKKRVKKKQTFHSQDIEKMQQSHKIQHRKSRFYENQICKSPAVIPDYGMDEIQIDKGYHSDSNLIKTVIPSSNQTSKVGIQDFEFIKPLGKGAYGWVFLVKKKGSGDLYALKIIDCAQRNLEAFLEQLKAERNIFEILNSNFVVKAYFSFVHEQYLCFVQEYMMGGDLASILKTYTALDEFYVRHYMAEIILALDYLRQQNIVHRDLKPENILLDSQGHAKLADFGLSEQGVNSRLKLKDSMNSFNTIEIPTCVEKMIDQQGYETVYKQLKKVESILVDKLGSKTKKIVGTPDYIAPEIILGTSASNFSCDYWSLGIIMYELLCGIAPFNDDTVDKIFDNILNMRIEWPQIGDAEDCISEQAYDLMIKLLEPDYKNRLGHKSIEEIKNHQFFKGISWNTLLSKPGLIVPELNSELKDTEKMQQFLKKLEKTNKDNENKKLTQQLKAQLQSLERIDLLKQRSIQESEKYLQQVKFEQQKLRKQIDSLTQFYAKIYQTYSKMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3R7</accession>
    <name>A0A8S1L3R7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0330316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L3R7"/>
    <dbReference type="OMA" id="NATIKHM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="65"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="247" mass="29916" checksum="84D8BFF4B7CF7B94" modified="2022-10-12" version="1">MLKKEEIWQSIKMFQKQNKRELDQTRESEDQSSFISDNEKENLFKNKQQSCQCKQQMLSLKKECMLQIRELKAQHQIEMENLQNQFQKLMIEQKQQYEQEISQLKEDLDFYMSEQNDKEIMLLIEQEQQKDKEIFQQQLEIQITDKLKLEQKLIQCQKELKQYKEQAQYFQTNQYTQPNQQQLQQSIHKNCAPQLFRDTNRKVKLQTEGIDRSIVNDDNSNRVGICNSIKNFNATIKHMEKSQQSQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5J5</accession>
    <name>A0A8S1L5J5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0300340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5J5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="288" mass="34930" checksum="FFEF06EB5E5F8718" modified="2022-10-12" version="1">MISSIQTVDSQVDQIRNSKQYRIPSHYEIEQKAKNYQIMTQGKLKENFPIQNQYNNQIPKQDNLNNNIQKTNTINIAQTQNKIYGQEMQQPLVQSNVDQPLIINDQQYKGINMKEKLIEFINCLKYFQVDITKFQQSSKNNTTEQHFHIKYLFYDLYPKVDFQIWAYNFQRQDLLQQLINLHYYFHLFVEYQRLIENNQRSSFQSTQIQLFINLMNIQIQQDKFLINKILEFLFLCKDRVEIQNYLNYIMHMTDPFQTTYQADPKQNQEQDHIYYLYGLYSHFSVSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L629</accession>
    <name>A0A8S1L629_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycoside hydrolase family 38 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0340061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8062927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L629"/>
    <dbReference type="OMA" id="QSYSYYA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000002">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000011">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000013">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF3">
        <property type="entry name" value="LYSOSOMAL ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="2">
        <location>
            <begin position="334"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8062927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="912" mass="107390" checksum="B15231ACF931384C" modified="2022-10-12" version="1">MIILLIIISIVYGNFLGKVNKVKSDYLRVFLFPHSHDDVGWVRTMMEYYKDQNGVKQIINSYMEQLIHDKTKHFSQVEIAFFSIWWNEQNDKMKQNVKELVKNQQLEFLSGGWCMNDEATSYYEDIIDQMTLGHKFLLQNFNYIPSIGWQVDPFGHSNTQALFSNMMGFNAWFFGRIDQEDRQIRENKKELEFVIHPDSDQRHSILTHVNYYGYYSSPKGFDFDITNPNRQEVTNSNLQQKTEELAAYFKQQQTVYRGNILAHTLGMDFQWSDAASYFSQMDRVINQVNKNTEKYKMIIQYGTPKQYIQALNEQNITYPSQQEDFFPYADYPNQYWSGYFTSRSAFKGYVRRIGRYFQQVKYFYSFIKINNQCKSLCDDKNLKNLAEALGTAQHHDAITGTAKQYVNNDYIKMIEIAYQNMNNQISTLLNSLSNTKSITHTTCHFNGTNVCHSLFDPLIKNQTVILTIIDTKVNNNGMKEYLKIYVPDNLYIKATYEQNNPASGEILCLNGQCILYLLRIVGNSKFLHNFKLQTVESNKDTHIIKIEPEKISIDSDTKLFDKFKLNYNFYYSSSGAYVFKPDGYSHPYGDYVKAYKFTGNMVKQIYIEKTAIKAWVTNFDNDENIFYVDTFVDSIILQDYKGQEVVIQFLTDINNQQIFYTDSNGLTFQKRQVNHRDSWQMKVLEPVSGNYYPVNGAIMIKNQDEDQACAVINDRSQGGTSLSNGVIELMLHRRLNNDDYKGVNERLNEQEEVNNQLVGMRQMISHIIVFYNQKSNPNIVRRLQYQQDLQPLIYFSTQKQINYQYQQIQNKFITFIQQQTDDNLCKFYIEPWINENQYLVRVHNFREKGIQKLNFPPGLRFVETTLTGNQELKNWELNRIKWTDNYNKESSQQNYDEDQVGPMQIRTWIVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L685</accession>
    <name>A0A8S1L685_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L685"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="47" mass="6002" checksum="CC03AE66C4EE896C" modified="2022-10-12" version="1">MEIINQEKFDKYLVNYFILNNLFRIPINPEIYQREDNSNYQYWRIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L7U2</accession>
    <name>A0A8S1L7U2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0320088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L7U2"/>
    <dbReference type="OMA" id="HDFFFQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="196" mass="23257" checksum="F4D6E914B89CA244" modified="2022-10-12" version="1">MTSIKDQPILQNRQKIHEILKNKVQSSPIQHFKYNSDNIKLWQIPEQDKRTTLISTRKSIDQNVTSKNLKKFKLCHHKHGSLDFEPFQLNNSTNRFTQIKSARSKKINHFDLSKQINNINNCQQENKIQTIKTQSQFQNEQIYDENILDLLLLNTMELKEILSVNNEPPKKTQRIKPKITYITNTKVLPHDFFFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L8E2</accession>
    <name>A0A8S1L8E2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0330190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L8E2"/>
    <dbReference type="OMA" id="ACLYLEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="117"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="697" mass="82133" checksum="9332E4EBB04E3828" modified="2022-10-12" version="1">MKEPILITLIVQADKENDFIVQFPLKKVNSMCQIERERLLRFLIKQHKIQAQDKTKVIFEFYDFQNEAFIQEKQVTIKNNTFQFSEKFLGSPEQLVLIVAIFYNFSYSKLIEIDQAIEMFEIDKRQFQEQNKQQQNQIQEYAQQFQEMKNTLDDIQNQLSTQKSQKQIEKADIVILYSNPIIRVNNGIKESLFVDYQSEIDKIVEAIAQSNKPIRYQILNATLNNLVLAINLHPIIIHVISHGDYDEKSACLYLEFQDDGISTKLKTDEIQNMIQNHKAMTRIKLICISSIVAKNIADYIPKAIPTVVFQKFYNQLDLEGPIFWGKFYEKILADFTIQSAYDQAKKDLKLKFWKNDSKFICCCFHKHFEKCPYDPASIGFEVSHSQHLSCVQQKWLHILIPCLPHTTVDEEQCEISCLGRLIHKCTKQCDNYAYQIDTILQKLQITQIDEDTLYANLQSIENKPYGCCCDQLNAILETIENSNQNFAKQQHTYDQKIQLHLFDENANNLNFSEKIENNQEQHIDINFIKVDKQIILVHSYNLQNTDLQEQSLKAISSYFQVYLKQMSIKKQIQKVQINLKDCNINNWIEYVANKLNFQYSNNQDFFIKLKDYFITLKNQVQYLYIFLIENIINIYASDQFKMFYEQIQNVIESHFILVFTVDNVQKDHLKSLTADKLIQLIDLNQLYSLIEIENAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LES8</accession>
    <name>A0A8S1LES8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0390009</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LES8"/>
    <dbReference type="OMA" id="VAVQTFH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="82"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8066280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="320" mass="37614" checksum="5FFAF5D16B2E8717" modified="2022-10-12" version="1">MIKKYLNKFFIYFICFSIVFVYIVNVKVVWIDLIGSSNYRIPYLLIFTILTTLLGWSLFKTMFTDPGRVPQNWGFFLNDPEQKKRKFCLICHIFKPERCHHCSACNRCVLNMDHHCPWINNCVGFQNRKFFMQMLFYVILDSYCAVIGLGYGIYVEFENIMLYLNSEGDLHFIDGLLLLCAFGISCLASCLITMFFKFHLQLVLSNRTTIENLEKKRNEETGQQTDDFNQYDLKPYYNWVQVFGMSKLSWFLPIQMEGGRPVGDGILWPKNHHNESLLLKDHASIPIYNGNGDNIQMQQYNNQLLHSNNQQFRSSSTQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJY6</accession>
    <name>A0A8S1LJY6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0390111</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJY6"/>
    <dbReference type="OMA" id="DSPHKFY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.440.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_eL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020052">
        <property type="entry name" value="Ribosomal_eL31_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01144">
        <property type="entry name" value="RIBOSOMAL_L31E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8066482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13828" checksum="567232A71091552F" modified="2022-10-12" version="1">MGKKEKGKPNPLGEVSRDYTINIHKAVHKETFKRKAPRAVSHIIRFAQKNMLTDDVRVDPQLNEAIWARGIRNLPRRIRVRLQRKKKEEDDGKGKYYTLAQYVPVDSFDNLKTEITKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-05-03" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LNB5</accession>
    <name>A0A8S1LNB5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="97" mass="11129" checksum="D9238C374F862E37" modified="2022-10-12" version="1">MFDPMYQSMNQILEENGNILWLGDCTAAYDRSLLDGRGIKTVLTVAAGLNVSYPEGGIVHKVYHILDIESANIXQFLDTQQYDQHNLNIKWRIKVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LNF2</accession>
    <name>A0A8S1LNF2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0420104</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LNF2"/>
    <dbReference type="OMA" id="YEACEWT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042512">
        <property type="entry name" value="TLCD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31898:SF1">
        <property type="entry name" value="TLC DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31898">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="19"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="46"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8068329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="269" mass="31558" checksum="617F7863F13064AD" modified="2022-10-12" version="1">MLNLENIDLVNSTSDQVKVMSYSFFIWTSIFAIINQKFHLKNHPKKVQDDIKNRIVSIIHGALTFWAAAYIILVEQPSFGASNSQTMQFTMIISASYFVYDFLACLYYDLADMSLVSHHSLAICGYAVATFSRFGAPSSIWGLMSAEVSNFPMHMRVIFRQVGLRHTKLYEACEWTYFALYIIFRGSLVPYMVWNTWPESEVPLLVKITATGLFLQSVYFIFEMKKILTRQYFQYQERKKKNIHHYWFSVNPRIQELSYINNQKKDRVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LW70</accession>
    <name>A0A8S1LW70_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0440256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LW70"/>
    <dbReference type="OMA" id="YTCSERK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="260" mass="30495" checksum="81F38C7DD35584E3" modified="2022-10-12" version="1">MTEKKTTADYNRLQNRNNQFKKCSDCLNNTISKNPIANIFLKMEQALCSSCINTNLYVIPKTQEFSDAITFPFTIYKKILCKIHKHEQRLKCQECSIYLNEKNYAKCPFCEFSIFRRNLKKWSILYPIHKCEVCNHYQCLKCQSIPGIMGNDAIECYTCSERKQDQYFHIKHAIKQMNTFKGIQFLITIFCVAIFTPIQIMGKTTSITKQIIDWQPLQQVTPRQYLFKLDHSYGYGYIQDTFLFCIGKAIPTTFACYIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LWW6</accession>
    <name>A0A8S1LWW6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0500287</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LWW6"/>
    <dbReference type="OMA" id="WRAEGHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000191">
        <property type="entry name" value="Hydrolase, NUDIX family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000111">
        <property type="entry name" value="Hydrolase, NUDIX family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003293">
        <property type="entry name" value="Nudix_hydrolase6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040618">
        <property type="entry name" value="Pre-Nudix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13994:SF13">
        <property type="entry name" value="FI03680P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13994">
        <property type="entry name" value="NUDIX HYDROLASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18290">
        <property type="entry name" value="Nudix_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="123"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8073258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="292" mass="34232" checksum="36596ABA249E61F4" modified="2022-10-12" version="1">MMKKLLPLALFTSYNKSFIKNVKLEILNGVQDDYQCFQIEKGHYLNQFLNNIEQFTTNIIETIKDCKSKQMKAIWIQLDSNQLALAEKLIEQGFQMHHCTQNYLLFSQWIIENEKSRLPNYTTHSVGAGGLIIHNNQILLIQEKNGTKEGLWGIPGGLVDDGELVAEAASREVKEETGLDVEPYDCFFFRDLPIANQYQGDIYFVIFMRLKNQQQKVQIQDQEIKNYQWVEINKLQEFLIQNKFGITQQRLMESIVQYQKSNRFGTQLCNMDMKPRVMYGKEKKYCLFKPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYA2</accession>
    <name>A0A8S1LYA2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0410091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYA2"/>
    <dbReference type="OMA" id="IPIKHCY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="409" mass="48518" checksum="F384D2D376C06673" modified="2022-10-12" version="1">MILFLYNNQILKLLKKFITNQNKNLTEKQLISIFIQDSLTQKILKGNLIRQGSLIQFRIFQPIIKINKYIVVNYIIIVFQFQRINGKRLLCFKCKNFFYDKKQSSQQCFSSLGNFESIQHVNDSNKWDDIKQSRQDLMKKYHQFKTNEVKQSNLSTYQSQKVMIPKIENFDIKLNIKRDTEIKNKDKENILCSQQKQQERSGSNSQRIRYESVYDRLYNLRDTENKGTKVVQKILNHNNNNSQKQSKQILGHNILIQKRSSDPSILMPISSLIKKTQKQQRDQQQDQKNAQNQIHNQQNTSQDPYRVNVSRQQVCHLEKNESNKQNQSTEIQSNLNFSQIPIKHCYFSLINAEEKLQNKKVRSLSSEKNKIKQSFKSQKGNRAHTQGKQENKNQQKINRLYEYILEGNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M391</accession>
    <name>A0A8S1M391_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">cGMP-dependent protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PPRIM_AZ9-3.1.T0520256</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M391"/>
    <dbReference type="OMA" id="GCFVFQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05572">
        <property type="entry name" value="STKc_cGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000154">
        <property type="entry name" value="cGMP-dependent protein kinase 1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000089">
        <property type="entry name" value="cGMP-dependent protein kinase 5-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035014">
        <property type="entry name" value="STKc_cGK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="19">
        <location>
            <begin position="112"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="19">
        <location>
            <begin position="231"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="481"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="20">
        <location>
            <begin position="741"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="512"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAD8074397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="802" mass="92694" checksum="B4E3E4174BBBF766" modified="2022-10-12" version="1">MGSVCKKQDAENELNSLIPKKKGDTITISQNHGGGSQIIQKKRITGNKGEYEPDAVVEKLEVELKRNDKKKLVKVEARQDQEEIVENIQKSNKKKSPFDYQLILNSFNTNSLFQSLSQQEQEAILEQMFYCTTPDGQFVFRQGDLKASSFFLIERGQCQIIIDGEVKKVLKQSDSFGERALLYNAPRSASVRAVGDCAFWAIDRNLFRKMIEDMRMHEFEENRQFVENLKSFEFLTFDQRTAISSVLFTVQFKKGEIIVQQGETATSFFIIKKGSVSVIQNDNEIRKMKKGESFGEMALFQNSTRGATVKAADEDVRCLVITRDELTKILGDKIQVIMFTNKQIWAIQKHQVLGQLTELQIQKIVQNMNQFNYEQGAVIFEEKVQKLVIVLRGEIIFTGSKMKAASEGQVFGDAYFNKQQLSDPVIAKDKCILAEIDFKRFEEVIGGSLDQVINKNQKISEKHKKQESYKHDYSHLKLEEFVSLKKLGQGQFGNVYLVKNPKDNPPHFYALKCISKAQIVDQHLEKHLAQEKQVLTSVHHPFLMKYYCSFQDTNHVFFLVEFIKGMELFDVIRDIGLLSAFDTQFYIGSLILCIEYLNKQKIVYRDIKPENIMVDEKGYVRLIDMGTAKFLNHKSGRTYTIIGTPHYMAPEILQGKGYTFPVDIWSIGICMYEFMCGQVPYAEEAEDPYEIYEEIQKKQLSYPQFLKDRKAKKFMEQLLNKTPELRLGGSYASLKANQWFDQFDWDKLYDKEMKPPYLPPQQKMPATGKDVQGTPIMKELQNLNAQVYKKEKAKDPNWDQEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M565</accession>
    <name>A0A8S1M565_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar proton pump subunit B</fullName>
            <shortName evidence="5">V-ATPase subunit B</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Vacuolar proton pump subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0500095</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M565"/>
    <dbReference type="OMA" id="HGQIMNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12240:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit B, brain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005723">
        <property type="entry name" value="ATPase_V1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01040">
        <property type="entry name" value="V-ATPase_V1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="7">
        <location>
            <begin position="38"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="6">
        <location>
            <begin position="161"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="8">
        <location>
            <begin position="392"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8072881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="510" mass="57020" checksum="652FFA0FF0FA2D07" modified="2022-10-12" version="1">MSHHHKAHDIAKVAAARLNAAAASRNYNVTPRVDYRTVVKVDGPLVILDNVKFPRYAEIVNVCLGDGSVRKGQVLEIAGKKAVVQIFEGTSGIDNLYTHCEFTGSTLQMAISEEMLGRAFNGSGVPIDKGPPVLAEEFLDIQGQPINPYSRVYPQEMIQTGISAIDCMNSIARGQKIPLFSANGLPHNEIGAQIVRQASLVKGKDVLDHSDENFAVVFGAMGVNMETARFFQTDFEQNGSMERVVLFMNLANDPTIERIITPRLALTTAEYLAYEKELHVLVILTDMSAYADSLREVSAAREEVPGRRSFPGYLYTDLSTIYERAGRVQGKNGSITQIPILTMPNDDITHPIPDLTGYITEGQIFIDRQLNNKQVYPPINVLPSLSRLMKSAIGKGMTREDHPEVSNQLYANYAIGKDTASMKAVVGEEALSAEDLLYLEFLKKFENNFISQGAYEVRSIFKSLDLAWRLLRIFPPEKLKKINKKNLETYYYRRKEDEDDFDGPQQQQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M646</accession>
    <name>A0A8S1M646_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0480208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M646"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="17591" checksum="C91365CE8D463F32" modified="2022-10-12" version="1">MPFIVQIKKILEIHQIYFFETKNAKIKVQYVHFLCQSIINNMQKTYFLQKLLYQILIKQILQKINKNYTNYIIKETKYLNIIIRLNKPLDKIQLKSKRCSINQKQILKNKLKYLIWRLIYLLKMLLKINYFFKYIKIKKRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8R5</accession>
    <name>A0A8S1M8R5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0470280</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8R5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="303"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="345"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="395"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="437"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="479"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="521"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="563"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="605"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="647"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="689"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="731"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="773"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="815"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="857"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="899"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="910"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="941"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="983"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1025"/>
            <end position="1066"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8071634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1153" mass="125867" checksum="189B76167FB2E184" modified="2022-10-12" version="1">MKGSFQVKEALADIFDQVKDVDIHIFGAILEIFRIEQVQDSIGYLSNMGNQRRLESKILQQVENITQVDKEQKFSVIGNDMKQITDVLKKLKYHEFNQKDFSSEENEESNLNLIQRIKDNKQILELMQFLVHLTSIDEALIQGGSNSLHLLVQMKVDLRIKNFENIIIQNTSLIGANFVSCNLSGSQFKNVNISGINLNRAQLFNCKWRELRIHELNQLHGHSNYVRSVYFSPDGNTIASGSLDKSIRLWDVKTGQQKAKLDGHDQQVKSVCFSPDGNTLASGSDDNSIRLWDVKRRKLIAKLDGHTSCINSVVYSPDGNTLASGSDDQSIRLWDVKTGQQKAKLDGHSYNVYSVCFSPDGNTLAYTGGSRSLFLQGDNFIRIWDVKTGQQKAKLDGHTSCINSVSFSPDGNTLASGSDDQSIRLWDVKTGQEKAKLDGHVLQVNSVCFSPDGNTLASGSYDMSIRLWDVKTGQQKAKLDGHTSCINSVGYSPDGNTLASGCNDESIRLWDVKTGQQKTKLDGHTSTVYSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHTSCINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHTSCINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHTSCINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHTSCINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHTSCINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHTSCINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHTSCINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHTSCINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHTSCINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHTSCINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHTSCINSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHSDYVRSVCFSPDGNTLASGSDDKSIRLWDAKTGQEIKSSDINYKDILAQFTIPLQQNCPLQEGTINFLIFQASNYITTLLISQQAIFQAQGALILKGEFINQSGIDLKTLFKQKGSCILEDLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9D7</accession>
    <name>A0A8S1M9D7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myb-like DNA-binding domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0530021</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9D7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF69">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="237"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="237"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="280"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="284"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="331"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="335"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8074531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="52897" checksum="57B5F5777B98A45B" modified="2022-10-12" version="1">MFVDLWLNFCCAKRSKQEAQDQPISIKEDSNKPLLDTGQGYQPSPVEQKALQSTSQELRQKLQEAQEKRQQQQQSQQQNQFQNMINKGAVKTSIEMEVEPVEGFLSLSDNKSNKMASWEALELSRQNDILNQLAQFSMLAKSISSNNGPYQGSNPGSQPTSDQQIMSGQYPQYNNVAQTQQLDVIDEYPKKQMIKSQSQLSLETGKIEKKRIQKDKKQLKQVNDKIEKKEVSHAKLWDKNEDAILRQAYINFNGNWRKIAEQLPGRNMNQCSQRWRRLNPQENNKKKWTLEEDKNISFLVQQYDKNWAEIAKHLPGKSGKQIRERYLNVLDPSINTSPWTKQEDEIILKVYKEYGAKWSVASKQLKGRPENAVKNRFYSYIRRVFLGQQNPYSVVLNNGEINGHDNTLSQDSESIKQALSMDQTPSFTASSYLSSFLDDDYMDDEDEPKEKEGQFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAD8</accession>
    <name>A0A8S1MAD8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0570272</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8077197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="251" mass="29924" checksum="E4543DD2A91B208F" modified="2022-10-12" version="1">MKDHQESHIRKLTKIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLIKINSRLSKTFYYLNFSFLKLQQIRQFSKIVEIQNIVFEKVISLRQANLRQCVLEVFSFFPLVQFLIGEYPALLGQQIKKHFDLYLAEIKYLMSFQQFRNMKIPVIYKNFLFMGIYIFRIFESSSFLAESQQVKKLFKWNDNSLAFGNINKRIKRFVIYQQVRINSRFYYRSLCFLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAG9</accession>
    <name>A0A8S1MAG9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0530150</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAG9"/>
    <dbReference type="OMA" id="ICYEEFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045191">
        <property type="entry name" value="MBR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937:SF65">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ARK2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="194"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8074783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="251" mass="29736" checksum="3647D8FA8D250CA8" modified="2022-10-12" version="1">MQNIQRLLIAPDSSRKYLIKQDAMNLLLSIILCLFYVDNLMNDQLPYSAAWFKCSYLFLLFISNLCAIFLYVLFIPQLITLKQEQNKKDQIIQLRTLLQYKLHQYLNINQCLINVLYIFGIILVIFDFFGESLEQTQNIKKLIRYSVCGYLLNRIFITGQFENTLEEQTIVEFKESFIKIEELKELSLSTLDICPICYEEFKVKEHVAEYQCQGKHTFHQDCVIQWLKTPMNKTKVCPYCKQLPDSINKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MB05</accession>
    <name>A0A8S1MB05_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0580193</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MB05"/>
    <dbReference type="OMA" id="KLYPCRL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039512">
        <property type="entry name" value="RCHY1_zinc-ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017921">
        <property type="entry name" value="Znf_CTCHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319:SF0">
        <property type="entry name" value="AND RING FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G08900)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14599">
        <property type="entry name" value="zinc_ribbon_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51270">
        <property type="entry name" value="ZF_CTCHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="CTCHY-type" evidence="5">
        <location>
            <begin position="78"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="143"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8077598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="252" mass="29690" checksum="34AD444689361BB5" modified="2022-10-12" version="1">MIKQINCKHYKRNCDKKAPCCQIYYPCRLCHDQNYKGPKSDGCRVETMDRYNVKEIRCRSCLTEQPPSNLCNNCGIQFARYYCEICKLYEDDKKKNIFHCEQCKMCRLGKREEFFHCEKCDVCLAISIKPQHNCIEKAFEQNCAVCMDYLKNSTQLIQQFTNCPHFIHVKCLEKMLKKGLYNCPNCNVAIYKMTQKEIQYLDQINEDMKNELSEEQLKQVVDIICSDCQAKSSVQFNIYLKCQSCGSYNTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBT7</accession>
    <name>A0A8S1MBT7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0590033</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBT7"/>
    <dbReference type="OMA" id="HDICGIP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035724504" description="RING-type domain-containing protein" evidence="4">
        <location>
            <begin position="16"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="344"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8077860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="431" mass="50232" checksum="FDBF7525079962D4" modified="2022-10-12" version="1">MRLLLSLVLIDLCCGNIVNTTENKSEENQEIDIGLQTYCKKIHCNTYTSCHSCEFKNCCQFNLENNQCLRLLKAPFNYSELIDRSKIEVKNISEYPDPQMNVTFPKCNIVNEVPLYNATNYLFKNHDICGIPKYCPNQSYYVSAESRIVFQNVKSYVGQGELCTIYFQGRMGPEFLIQIGIGNLSLKEVKADLSICLSVGLFDDFPCIDSLQQIKLTKNLYYRIRANQFRITLQFTEDADIDFSQIFYQIISIQKLSQQNQQQLFQFIIILLLTILGSIVIMALIRTILIRIFGRRFVEWLDPEPIFPHRRRNFNVLSEFERLKSQNLIHQIKQNEMKFEQDRCAYCLESFQTGIIIEIFCGHCFHEQCIQNHMQIQKNVGRCSICLVDINKMNLIDKMNQTLQQQQAAQESLANQSQIQENQSMINNNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCH0</accession>
    <name>A0A8S1MCH0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0540179</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCH0"/>
    <dbReference type="OMA" id="CLDIHIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="246"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8075385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="37901" checksum="893DF50E4D499F6D" modified="2022-10-12" version="1">MKSKGDQSKGNIKQQPEKQGNTYKEIQCHYHPNEKLKYFCKKPDCLQPLCKICLDIHIEEHQEQNDTKDVISYQKCLSEVSKKMSDNLEYFSDKIGQLHNYIDRIINFQCPQNWMNLIQSSENQIIDLIKSYFSNLKSYLFSTTPEINKLYSAQDRLIICSTRLDQLKSELENLKNENSVTQIILYYQGDVEKLNNKYLQYLEQTFDELKQIDFSTLNAPKILINHNKLNNLLELLNEYINYEQQQEVQEEQEEEEQVEQDQQDEEPQIDQSDQREQTIQNDQSEREEEQPIDIIIAQEELQDENEYPQISNEEDLEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MFL4</accession>
    <name>A0A8S1MFL4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0610036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8078928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MFL4"/>
    <dbReference type="OMA" id="HSPQIGR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8078928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="106" mass="12189" checksum="8EF101AAD69FB636" modified="2022-10-12" version="1">MGCIAQKVRSQPRDQGRTTYPNSQSQNQLNQQSKILATEPQVGRVNSEINVPHSPQIGRRLKHMVSSDELQKYITKRQSITQSPFKYIKNSPEQRRKLSLMNSPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MG02</accession>
    <name>A0A8S1MG02_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0560120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MG02"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="164" mass="19396" checksum="33FAC23AD44588AA" modified="2022-10-12" version="1">MLKSNKLTSESALTTNRPTSRIDTSQLNRGYSQYTQRQSTNKKLWIKINKVTEDVTDSKPYIAIEKQRNTNSCHIRSSLSRKTSSDIKNQIDYNEIKHYIKEVDHNYEDEPFSKKDNQKLILQYQKEKRLNHLNLILATFRDRIKSRNISNDIESNNQQSIYII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKP6</accession>
    <name>A0A8S1MKP6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0640206</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="RLTSCKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003559">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003672">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF12">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="1049"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="109"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="316"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="796"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="66"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="116"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="131"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="328"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="796"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="808"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="840"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8080870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1371" mass="156327" checksum="4658959887B9A536" modified="2022-10-12" version="1">MQSISSEVLKAGVTKSNVRSNVTAKRPNQLGGGRTQLGPPQTGKDTQQSSQDNVIVKYKDSVVTPKELITKRFLQLAEQLNREEEQKLKQLQKPADTKVSASVMDSNMKSFHQSADKSSNSDLKVSSSNNPERSSFMESKQSDTYSDSDSKRDQSEQQEKKPKQKKGLKEEDLEENIPINLSETPTQCLMFIPSSFVNSEHGKKTGQIQRTQKYEQFIVEKIGSDNFTKRFAQTFNYIQKHKPQQCTKIEKNDTGAFTAVWDLYDASITDQLNEFEILQEEIKQSIENEYKQLNKNPYFLLPTELEAIKIHAERPVLGKETSEKSQSKTRSGIATNTKQQPINNLSGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIFRPSSFVNSEHGKKTGQIQRTQKYEQFIVEKIGSDNFTKRFAQTFNYIQKHKPQQCTKIEKNDTGAFTAVWDLYDASITDQLNEFEILQEEIKQSIENEYKQLNKNPYFLLPTELEAIKIHAERPVLGKETSEKSQSKTRSGIANNTKQQPINNLSGGLTGQGGDERIQDSSSSHTGTKGVTNMGSQQANIQQQVTKSHGKTSNPLPEQQQKQYEQKQDEQDIKEEEYMKQYVQRTNSQISEQEQIIYQSQQVQTSLKYVERILNQNLYHKQYIQYRNYPEVKFEKMTLNEDPKQRGVAFKRNFQNKLEDEEVVEKKQQDPITQLFSYKCQLSEQCKVTSADWNPVNKDLLAVSYSNRETSDGHIMFWTLKNPTFPERIITYPSKINCCKFSESQPNLIAAGTVDGIVAVWDIRRKSNKPITENKEMAGKHSDSVWEVQWVGKGAKGTDKGESLVSISSDSRIVEWSMKKGLEYTNLMQLKRVVQSSQKENLQEGVNFRMSAGFSFDFLQRESAMYLAATEDGTVHRCSKSYTEQYLDNYFGHSGPVYKVRCNPFFGDIFLTCSADWSCKLWNWTEELPKANFQQQNLQDEVLDFEWSPHTSVLFASVCKDGRLELWDLTKNNMLDPYFTIMPQDQQIWPAKTMVRFAQNSPVLITGDAKGDINAYRLYGYEDNDPLQEEEKLRKLLYPTGYSKGQKEQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTC5</accession>
    <name>A0A8S1MTC5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0700135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTC5"/>
    <dbReference type="OMA" id="KTIKGPF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="240" mass="28108" checksum="018B39D2AC79F90E" modified="2022-10-12" version="1">MNQLALDKVKQFESNSGLNSPLSMTFRLSTTLRQHSRQMKRQKSRSQQKPFVLHQKTIKGPFRRKSCYCDECGQLGTFQYKCMNIKGPRPYRKAILTRRASNQHIQSAQPKSVQRIKQALKKMKTYDESKINAMQDSNKNPQTLQVIQEQMTKLHSYFSRQQQQQIKVYQLAQYTPNCSPKHSKTPRNLPYLSPYVTLRKIDSQLLKPLKPAQLIQQRSQLLTMHRTKYHQKTLSTVSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWK8</accession>
    <name>A0A8S1MWK8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquinone biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0720176</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Membrane-associated protein that warps the membrane surface to access and bind aromatic isoprenes with high specificity, including ubiquinone (CoQ) isoprene intermediates and presents them directly to Coq7, therefore facilitating the Coq7-mediated hydroxylase step. Participates in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the COQ9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWK8"/>
    <dbReference type="OMA" id="CAGFGWN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013718">
        <property type="entry name" value="COQ9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012762">
        <property type="entry name" value="Ubiq_biosynth_COQ9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02396">
        <property type="entry name" value="diverge_rpsU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08511">
        <property type="entry name" value="COQ9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="COQ9 C-terminal" evidence="9">
        <location>
            <begin position="118"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8084620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="221" mass="26178" checksum="80C91861E0DC0F59" modified="2022-10-12" version="1">MYKFIAKFHFCDIRNKLFNLSLIHAINHGFNNKALQQACLDLNISSAASTVIQPIDLIHYSMRKWNREIIDTMTFDENFKQFTVPNKIKNLIKQRLQLQGPYMGRWNEAMALGAQNANQTSAILWQFADDSWYLAGDKSQDYNHYTKRMMFLYIYISTELFMLTDKSPNFFMTWDFLDRRMSEIKDFGSQIELISTTMKTLWNSGYYMTTMFYNFPKGTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZ34</accession>
    <name>A0A8S1MZ34_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BART domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZ34"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023379">
        <property type="entry name" value="BART_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11527">
        <property type="entry name" value="ARL2_Bind_BART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BART" evidence="1">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="70" mass="8734" checksum="17247C3D3CA4173E" modified="2022-10-12" version="1">MGFLNYFFSNKHKVQYLFRVYPKKQNQIWIYDQHLTSPIWKIAISEFWVENCIIFDDEDQYKRNIINLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0X8</accession>
    <name>A0A8S1N0X8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0700044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0X8"/>
    <dbReference type="OMA" id="KSCIVER"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000808">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="215"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="26039" checksum="D2D83FBF3A894952" modified="2022-10-12" version="1">MKQNQQLELSKEKVPLFKFILIGDQSVGKSCIVERFQNEQFSYKTKATIGVEFIKKLVQVDKGLVEIQLWDTAGQEQFRSMIRGFYRGSAAVIIVYSVNQKESFDQLSIWMNEIEQHAHPEIIKVLVGNKCDLPREVEKQEAESFMNNNQFSLFFETSAKTGENVEEAFVQAAKLVLLKYLSSESFRQATRVNKKSADPINIIKSSNDNQSYEGQSPSQQKNSNSSGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N127</accession>
    <name>A0A8S1N127_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N127"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="309" mass="36130" checksum="445790C65DC32558" modified="2022-10-12" version="1">MFDQQIKSLQCAAIETQAYYDIYWQVPSKQAAAYNMLLDKFNNTKIKIEVDYNQIQLHPQIMEIINYYNHIQTSQIPLIKATTEIVILGPPSSDPNQHYNRSIQKICSKLLNENLLDDLSNFTIEKQIFQNLNNLIHCQGLNTVILQPCTIPVLSNYPLWFSQGLIKTLEAYQVGYLELEEIDYLTEIQIKFIHSSLNYIDSKLKQKNSIRSKNEPNTLSPYKGSNIHQSEIDNQQSKHRTKSILVSNWFHFKTYSNLRPRDTQSQIKRHKAVSSSQVDLHTPFSPQKQSPFNLKANIKPRWFEHIYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4Z1</accession>
    <name>A0A8S1N4Z1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0670091</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4Z1"/>
    <dbReference type="OMA" id="FFEFEWQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034870">
        <property type="entry name" value="TET_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23238">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="9">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="9">
        <location>
            <begin position="109"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8082114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="146" mass="17056" checksum="402552F012645B90" modified="2022-10-12" version="1">MAQQKGKNKINHRQRQGDWICSNCNNMNFAFRDSCNRCNTVKNMKNNESKGFKSALFLTESNGDIPPISDRSNKSSGEKTDIGTNKFSFDKLPSMEPILKQITKETQPKYDFFEFEWQCQKCQKINQYYKIHCVECGAQRYRKSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7A2</accession>
    <name>A0A8S1N7A2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0750099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7A2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF2">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="206"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8085846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="442" mass="51520" checksum="B3987314D58A469F" modified="2022-10-12" version="1">MLNKHYKNISHNQIRITKTNYKQLNTQICLILKKAIFYKFQVFQIWSVGIEQQHVCFLFNGNIQDQFDNNRITYHTINSLDNKIDQNTYTIVYIVAEHNGINELSRFRKILKQLPSKYYTQLKCLFLYQANLLVKTRLWLQTSKRWSLWQKKTKYVNDVEDLLKIQNFQASWMNLVPKQTSGLDQIEPTQLVKQTGGSFGSNLTNQKLNQFGIPVILDCLLSYFVTNADRFLKPDIFRKQGSINEEEQLYTLLMNENYECLNVVEDVRIVCSMIKRFFTSLPDPLIPQNTLKFICNQIVQITPQTEIALLEEVFGKLPSLNRVLLTVMVSFLLCVGNYSEFNHMNMNNLAIVFAPCFMRAQTNDISSLEQVGQSVKFLKILFDNFDKMFQNVTFKLYLQYKQTQSCSSTSSNSSTGPETMSKNQILSSSEDEIVPQFQQKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7K4</accession>
    <name>A0A8S1N7K4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0780034</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7K4"/>
    <dbReference type="OMA" id="AILMVYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="282"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="474"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8087109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="546" mass="64371" checksum="2B590E42C37ADFDF" modified="2022-10-12" version="1">MRTTPNSFKETASKTSTQDYKKQQSQPTVSIPESSQLFHIPQKYCPQRFKPQPTISKSIEDKRCRVNKNNLSELNNRTNSSSKSRISGFTNNQLEDDSMYSSQNALKLKKIIGDSQAYTTNLFVSKSNLTCDSSSPGGRQNKSAYKKSIDFDINRFNMLKEKTLMQKKAQSQQQVNQSKELIDQFLNLDKQIQGSTLEQQKKQQYLKQRGLWDPFKDLFIKQFARVPIQFKSVFNQLQIQVREIEQQWSDIIMQIANNLFELLQNSAILMVYEQDLKLNELIEQMKRERDFWQNNYKSLEQERDILLETVSQLKQTLEKERPLQFNKTDKKQIDLDSVNTSQLKEMTILMQQKIQEMSEKEQKLIKLVLAIRRTGIDIEKIYNEEVLNDDSLSEQQNQSNRENKQLIPKIERSFHDADNSVVNDSDESSFQYNGRLEDESIIESIRRFENRNQNNIYATESKSSVKMKIDLSKCNQQQQKIQQLQQLQQQQQLKKIQQQIQQQNINKLKIPDQEGIGFHQEFMMKFNEFSESWRVQVLKDEKKNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N837</accession>
    <name>A0A8S1N837_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0820112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N837"/>
    <dbReference type="OMA" id="HKQVDKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="499" mass="60004" checksum="94B0CD4EA1740FB9" modified="2022-10-12" version="1">MISQVQDLYQLIYNKLVNKESLESSLLTKLHTQIFLQNMEKNYELEEFYYDILRKHIKNHYSISPEQLEILINIVYYLGIFINQQSLFTKAFQIIKENLIDNQPIIQNSLYLHILQACGSQNIYLKPGHQFFNIDINNNYFKSRFLTPYLNSITQRIPKDNIIQFIKDEQQNIKLLHPQSEDYIINELKLFIIQQQLKTIDFKQNSQELFDLFTFLQIEDRQQYNIKQKLINIEEKDNQINPFYQLAIKYKEFLLQYLDLNFEQFFEVFKINLNNIQSFYQNEKHMLIALNETIEEYLIKNNGCLQKLIEIQDEEVGYFSQCMIQSIDHFITLMMQNLFKLVSPYKSNFNISLQIAKISKMSKYHHKQVDKFKLKELEKYLRIAQNSFRPLYLPYPQFISQTAEIIQDIPYQLLKYLPKEQNKRYQLCNYGYAEITYNENYTLVINTKQLVSLFHLINNNIKQVDQNNLIMFKKIGLVNIDGNLNQDWKPTHKCLILFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9M5</accession>
    <name>A0A8S1N9M5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GPI mannosyltransferase 1</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">GPI mannosyltransferase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0820088</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of the glycosylphosphatidylinositol-mannosyltransferase I complex which catalyzes the transfer of the first mannose, via an alpha-1,4 bond from a dolichol-phosphate-mannose (Dol-P-Man) to the glucosaminyl acyl phosphatidylinositol (GlcN-(acyl)PI) intermediate to generate alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol and participates in the sixth step of the glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the PIGM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9M5"/>
    <dbReference type="OMA" id="MLWFIGQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990529">
        <property type="term" value="C:glycosylphosphatidylinositol-mannosyltransferase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051751">
        <property type="term" value="F:alpha-1,4-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004376">
        <property type="term" value="F:GPI mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007704">
        <property type="entry name" value="PIG-M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886:SF0">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886">
        <property type="entry name" value="PIG-M MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05007">
        <property type="entry name" value="Mannosyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 11" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035863402" description="GPI mannosyltransferase 1" evidence="12">
        <location>
            <begin position="22"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="144"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="315"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8088802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="380" mass="45005" checksum="E2945E050542A1DC" modified="2022-10-12" version="1">MSPKIAIIIGLLIRLGLIAYAEIQDKYFNLKYTDIDYSVYSDGAQYVVDGGSPYDRHTYRYSPILAYILIPNVWISSFGKILFSFVDMLACVFMQKMVKSTFLLNLWIFNPLTIQVSTRGSSDTIIVLLIYVMLYLLKKEMYTWAALIYGFMVHLRIYPIIYAIPLYFFIDSHQPERIYLGIISKNKVKFALISGGLFIGLLILFHFIYEDFLFQTYLYHFTRKDNRHNFSPYFYQIYLSFESITRTQATLTFLPQFLIVILAGLKYYRDLPFAMLIQTLGFVVFNKVQTAQYFVWWIALIPLALQNTKMNNKEILMLSIIWLILEVQWNFGSYYLEIQGYDVFTMIFIQCIMFFLSNTYLMIKFIENRKPSEFYKIKMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAC1</accession>
    <name>A0A8S1NAC1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0760243</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NAC1"/>
    <dbReference type="OMA" id="QINNMIP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="150"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="313"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8086503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="378" mass="43869" checksum="A4A0244B1C9753B1" modified="2022-10-12" version="1">MDEQKQNKIILKILVKQALIQQYLININVCCLLNDYFYTIALNGLAIIFIAFVYHIIPLKSSKIKYIVEYSLLAGVQILAIFMKELLIYLASSLLGLSILSVLFKICRTQLNVDQSLGLFKLCVGMLKCFLSLQLLFLSLHLNSVLPWSWMQIFVILWFILVCGLLFQICMILNLLYKTLKRLNYSIERRNSTNIKYYGGIWYTIQLHCLTFNPFFTLLNFCLTMDQNFTMIYLPIMCITGWMIMMIVSFIIRKEQINNMIPQVGTSENEVFMNEGIPKYLMKISSTYFQQTDKPFEILSTSPRKSNDAEMKCLICFENESGYVLMNCGHGGLCLKCASNLLLKNKECYLCRQPIMKVFQIQKNNFNFVEVIGIIETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAZ5</accession>
    <name>A0A8S1NAZ5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin-like fold</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0810012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NAZ5"/>
    <dbReference type="OMA" id="EGPRYIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050553">
        <property type="entry name" value="Thioredoxin_ResA/DsbE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42852:SF18">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_47, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42852">
        <property type="entry name" value="THIOL:DISULFIDE INTERCHANGE PROTEIN DSBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="933" mass="109604" checksum="D184A70D9ACB8F9F" modified="2022-10-12" version="1">MKNKQKSKFGQQLIASLPSYLNILEITNYIAQKEQDQIQIITAYKSRNRLKAADSELYQSIIEKLEYSKKDLDANEFELMAQFLNEIQPKYEIGDQIKKISDIEQIISSNNNLNFQTQKVTLQIQKEFIYVINIWDTIVLGYNGPIDEVVQLHQMKTEWHGTVKLIAISLKSTTEEIIELVTLKKWQYLDHYKLIKNKINNHQFLNMLNIEGLPFALVIGKNGKIIHAGYHYEVALDDLITQEMLRKDEDAPQLEELKISNNDEIFLDNKIINELQAKNQEQNMVGQKQSNDNRVLNISQQNKSNEDKNYEKFKESKLQNANSMIKVQLKVLKQYLLDIQKVLKNLHFQSKFQIVLRRQRRLRFDGCLMIEQISDLELEYQITYQEMEMLDYFMEQVWNKIPEHLRIVKVSNLYSRSDIIKNIISVAFAKHKVSIKYQQIMKKSLIWDSNSQEMIINNSEGYTVQSSGLNLENFYEGIQKAKLSIEELRKDFDYCVEINEVIEKLQSNTTLGRGKKFAPILNYKNFGSSKKESISHIKGQVLVILYWQCNKDCIVQLRNIDELIIKNNESWGKIVKFVALNIGNEKEYISFLEQNKEHQSRLQIYNKERAHLTDTSLYAITQVPYFILVDKFGFIRYIQSPINLERSIQQLVKENERDTYQKNLKAPIQQQSVDLIKRIISSNNFKTQLLSIDQNRVIEFRLDFEVQQDDKAKNAYQNIYLNYFIRDKQEEEFNQLLDKIFSIIPEDRWIIRKQIQQTVSIQYPGNKCVVCSKDISKEPKQYYCYFKKEHVCVECAEFTDLSQKGMYMYKYQDTLIFINGPLHDESVLHDIDSHKIGKNRQLQKGQKASQKHDFVCDGCVISSEGPRYIAVNARPGNYKKDGYIDYCKNCFQILKNKGSSEAKNIVAKNSHQGMTADSIFTRVLFNYGKYRDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDH3</accession>
    <name>A0A8S1NDH3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0800219</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDH3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8088366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="279" mass="32094" checksum="E90CBB47D04E3272" modified="2022-10-12" version="1">MFFQKQGVALIMLNSEECAIKIEKSPGIGQIQNEIGMLNKLKGISGIPVLISYGVTPENKSFLIIPLLHGNLGEIAKKKQLSLTQILRIGLRITEILEKVHYQNILHLDIKPENIMISQPLVNDSKILHPDLIQLIDFGLSQELIRNSKLLKDVFIGSLNFASRQSHKGEQLGYKDDLESLLYVLVYLRNLTLPWFQKPSWGYREVDIKVIGRIKQFHFKTVSQTQNFPLQFQEIMSYIDTLKYDIMPDYGYIKSLFMEMIKKPQCSIILNTLSILIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDJ4</accession>
    <name>A0A8S1NDJ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0800030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8087991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDJ4"/>
    <dbReference type="OMA" id="NLMTFEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="183"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="502" mass="58725" checksum="2DB278A7120CAA2E" modified="2022-10-12" version="1">MKKNNQSTITDIHFEKLQRPDLLYTRNQQNTQQCIQLLLKDPTIRLEQENEKLGQLLRGHEFFKTSDNEFLIECCQLMRFEQFQKNQVIFSKNQLDQNKLIIILSGDVVLNKGNQEMIHLKHCGSNLLLFGSECYSDISDWNVTSKTACKVAAISKHDFRVNLMTFEVSRITKEISQLYAFPIFKNVPYAQVRKFYANSMSQTFQCLDIVYLQEQMIKQIYLVIKGIFELREQEKGIAKAVHIFTQGQLIGENELNNTTSLCNGTAFCISEEGQLLIFDYDYFKDKVLPQIEDNLSIKQKTQITKNEKFFNNKVSLTGEIISKKHLSRHYNFKKSNEDYDEQQKQTIIQKNKIRSTHSINSIHQSNNSEELNESQINNLNSNLNTSQVKQSIKQNRLRINQRNTQKSIHFSNEQIRSQIINSPIRSTVNKKSSLQLSLQKLKEDITIQKSQQQMNLRMPLKNELLNKFLNRKEEYGDIVSKNRMRRTLSQTTNVKKTVQINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NG63</accession>
    <name>A0A8S1NG63_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0810096</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NG63"/>
    <dbReference type="OMA" id="MIIYSHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="499"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8088493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="901" mass="105660" checksum="E1FE83172A0D3A98" modified="2022-10-12" version="1">MIDDLEQTLYNDLKQAGSSRISDSFRPQLIHLDLQDIPFKENSESDSVPQVNSIQNLRFKDISISKSKNKDMCSLRSFQCQNNLQDKNDRQRHPRNSGISKMLAINNLVKQFIERLKKKAYIFPSNLGEKIRENLREIIHKKKNQKRLKQIQISDDGVKNQIFRKLPVFNPASKVMLIWEFFRAIQLTILLWWLPFKIAFNPSSSRNIDAFESALTYIFAADLVIKFNRGIFDQGKLIKNRISILKHYVQNEMHEDIIYFITLIFVISDIGIRASGFQEVIVLVQFGLNFIKLKKYLTKYEETFVESSILTEIVKLIQLIIITFYFAHFMACIWYYVGIKSIEVNEISWTQDPKFDNAGILQMYVYSFYWATTTMVTVGYGDISGKNIYEVLCAIVLMIFSSGIFAFSMNQIGSIFTNMDAQKQQYKRTLLLINQYMNNNQVAEQLQGRIRNYLKYHYHKQEKLYKNEISGIIDKLPVTLKSELIQDVQFRVMQCIPFFNKNFSEEILPQIACELNLQSYTPREIIYQQKQIDECNIFIVWKGEVNLIDDNSGKVLKTFTTGQCFGELEFLTNQKRLGTAISCDFSQIYYISRNQFLKILNSYNNDFQKFHLLKDQILFHYNSSAIECYCCQESHSIWKCPYIHYKPDLERVIKKSFFYDSSQDRSQFKRRTIRQAVYYQESLLQSSKHIKTSNDLDNATAGDIQQIGSINESIESPDESIRHEKKSDQILTLSYGLPSENRRISQRTKLVPASPLITESPLRKPTDMLRYGQKQRKSLFFAEQSVFTALQSKRHESVSKTTSLGQQQNLIKQSSNYENDSNPEPLNIIEGLDKMIIYSHYFPDNNYDKIIESILKYLKKYRLSRAYMIPSKYSFVRLQKYDKMKSQGKKSLGIKQHDFMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJY0</accession>
    <name>A0A8S1NJY0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="5">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0770174</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="14">Carbohydrate degradation; glycolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJY0"/>
    <dbReference type="OMA" id="DMIFDIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00318">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000003">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000006">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF0">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8086944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="419" mass="45446" checksum="21065EC6EFD87777" modified="2022-10-12" version="1">MLSSKAGIDHVLRQFTNKRVLIRVDFNVPIKEGKVKNTTRIQGAIPTLKKILEQNPKNVTLMSHMGRPDGKRVEKDSLKIVVPKLEELLGTKVNFVNDCVGSEALEASNAGNGQINLLENLRFHIQEEGKGLDANGAKIKADKESVKKFRKELSSLGDIYVNDAFGTAHRAHSSMVGIDHKIRVAGYLMKKELDYFAKALETPQRPFLVILGGAKVADKIQLIKSMLDKVDEMIIGGGMAFTFLKKIYNVPIGKSLFDEEGYKIVDEIIAKAKEKNVKIHLPTDFVCGTGLDASSPVALHDLKSGIPDGWLGLDAGQLTQRENADAIGRAKTIVWNGPQGAFEIEQFKNGSVSMLNALVKQTQNGATTIVGGGDTVNLVGANKANDKLSHVSTGGGASLELLEGKILPGVEYLTNIKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPB0</accession>
    <name>A0A8S1NPB0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0950001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPB0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="63" mass="7337" checksum="7473B7E85FBD2DC3" modified="2022-10-12" version="1">MDKTVSLQAEKDLCTQYFDDGKCLKVYLKYKTAIGIFKIIQVRKTICENSSFTLEQYLSECKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUL4</accession>
    <name>A0A8S1NUL4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0860178</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NUL4"/>
    <dbReference type="OMA" id="CFDKYKR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000134">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="30"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="94"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8090724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="348" mass="40039" checksum="ABAAEDB38137CF59" modified="2022-10-12" version="1">MFSLSPIKQNQYLSASNQFEQPLDDMSCIIENGSVGSLYLGNIESASSLENLKKHKIKGVLSICMNKIPFEVQTSLQHYLHIYLEDCESENISRHFENSNQFIDKARQSGNVLVHCMAGISRSATLVAAYLMKKNNMSAQDAIRLLERKRWQVYPNNGFLRQLQQYEKVLLQQNGRSDISSPLRDQWSKQFQTPTKDSLFYNKYDEQQQSSQKTRPLDEICFDKYKRKSVGQGDLIRSPENNEKKPFSLTQHRPSSAIRTTPDLMKKKNVLSDAIHGNKKDGLAALALELNQIDWQSKKLDFESKYSKPSIQQNNTKSFLSPTRPLQLNQHQSKLDELLNSFNRKSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZB7</accession>
    <name>A0A8S1NZB7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0980181</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZB7"/>
    <dbReference type="OMA" id="PHIRYIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="35165" checksum="DEF91686BE804C90" modified="2022-10-12" version="1">MGGGQSKAQKLQSQQRANKPQLPQHQTPKQHNQFLISEPIQRYFWNSQFDYENLISEILFSISIKRCMEYLQQFKLHMRYIKKARNYIMMLHNVVYMNQNLIINIKNNDCVLIPFFSLYHISKFQVIFSSSDKKWWEGKYFVITQKKYVKLYFIIIFIFLIISKILYHLKRKALFKLLFFLCLTDYQLQFLYSDSISYLTDIDQFVKEQELIKQQNYQTYRRSKLESNVEKQKQQQQLLYQQQLQDSKIPTQQIDDMEKQFKFLSDHSLIEIQEFSQKQKQVQETQKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P049</accession>
    <name>A0A8S1P049_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1020013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P049"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="154" mass="18593" checksum="70F31DCEE109A8EA" modified="2022-10-12" version="1">MGKKLKVGLSQMKMFRNKQNFLWLEIKQMKSSLSIWVLINNKSQSFYKQLKYAQKLIKARQTNTSYEYNEFLTNCQYHDLRYCKQQELHRSGLEEKLIQNFVMHANFKTTQVYIQENQQNDVIDFHLTETILNDQEVYKSAELILNFKDDNDYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P304</accession>
    <name>A0A8S1P304_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1040012</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P304"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001126">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000818">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF136">
        <property type="entry name" value="EIF-2-ALPHA KINASE GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="175"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8097380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="537" mass="62754" checksum="F3CB00AED7336117" modified="2022-10-12" version="1">MNSNSEKANIDSIIDQFYGFKVIEEVPEEERHFDQVVPQPDQTQIQKMLDDSLQKRQEFIQSNDCKEYSQMLIISQIRMDFTNDDPFLKHLMNVEKAIKKAIRNILLQLQKLENKQQFKEACDKLIKIFYQDINIGFITSTGQIKSTAYAFILKSIIELINRKKINLQFKKIPLKITLTFFAIDSFQQIIPAQKIYDPRNAFIKNNKFFNDYIILSQTNQDELRNGISIFFVYRPEDDQIYALKRSKLQATQLDFFSVFSTTASNSSITPDIRKIREAKILSKLTHPNILRLFAWWIEQTNDGYYLYMQLEYCSFPGYKYQPTDLLTFSYYYLNVMQNSEKINKIKSILNQILDGLEYIHQRGIIHRDLKPENIFVTINIKGDLQVVLADFDQGKDVREEKLSTMTDERLPQEELNSITSQNTITTGTCGYQTANYVKDSHYGMADEFYAIGIILLHLVIAFPGEPKNRNHYAKTFVMANNAQDVLTLFDTWANRFVKNKSTDFSFTHYENVMVLAKLLTSSKKLSHADVRKMINEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P328</accession>
    <name>A0A8S1P328_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1030187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P328"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="96" mass="11801" checksum="4D10AEAEB749FD0C" modified="2022-10-12" version="1">MFFRITSYYRGINEIKGKALVNRKRFAQIQRDKTRSVFPFYFFLMILKQKLFKIIQMAVNFIGLINKTRSGKGKYICYYSKRIFGWQFQWIWVNDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P3J9</accession>
    <name>A0A8S1P3J9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1040127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="722" mass="84221" checksum="40D5E6C5F6B284A9" modified="2022-10-12" version="1">MLFCQEGDHQQSEIIGVCLNNVCSKQGLCCFYCFKMFHQNHINDIMNFKDFINWKKRKEQTFNIFKENINQLQQCAKLLSDFISELVIIDEQVENQSYQNLTKEIKHLIIIDQLEKSIMYKVSELASLIPPLKLEIQQLKSSRGQYQIKNDSFSGSIDCISQQGYSEERIIQIDSIVIPIQKQQLNFTFSNKNKHEDILISYQGKEAKSSSIFIQTRYVICNELITENSFARIKVQCGESVSIGVCLQNITFQQMKPYEAILDGAYILNHEGVITTYPKDFKNSDQKFCGIGFGDIIIVEVNMRERKITWINKTAKXYKFKIYLQKSIFFQIFGFINLNKVERIILRFQNYYGQQQRNLIIQFYFFNFQLTDMLFCQEGDHQQSEIIGVCLNNVCSKQGLCCFYCFKMFHQNHINDIMNFKDFINWKKRKEQTFNIFKENINQLQQCAKLLSDFISELVIIDEQVENQSYQNLTKEIKHLIIIDQLEKSIMYKVSELASLIPPLKLEIQQLKSSRGQYQIKNDSFSGSIDCISQQGYSEERIIQIDSIVIPIQKQQLNFTFSNKNKHEDILISYQGKEAKSSSIFIQTRYVICNELITENSFARIKVQCGESVSIGVCLQNITFQQMKPYEAILDGAYILNHEGVITTYPKDFKNSDQKFCGIGFGDIIIVEVNMRERKITWINKTAKNKIFNLIINENVQGLYFIFGLTSNVFDSSFLKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P975</accession>
    <name>A0A8S1P975_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1090064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="353" mass="41268" checksum="AF8E3DA53A484407" modified="2022-10-12" version="1">MGVCLSKKLKMNKQQSPVNKNLEKTFQKTKYYQEKYMKEVFDSEKQCEIKMVNVKTSQSTDAALNNSFLRRKALSKSRIGLSTNRKGAIPILNTKQVSFKDSFKQKYHKVIGLLLSNSNLSVVQNNQNQQLAICQQLRKNDEKDQSFIKWLKEKNIEHDNIVGVQEIQVDDQDYYIIYEYWVDQQIQNKTELEKAILINQMIESIEYLHDNDMRHSALTLNSFKLINNKPFGVLKLIELNKVHELSELSIESWKYLSPESLSALGNRSKARDVWALGIIALELLMNEHPFIKNVKEISIRKIMMKINSLNNQQIEQMLNNLNKDLKQLISGMICIDPXFNQKLIIFRHNFQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCS2</accession>
    <name>A0A8S1PCS2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1140110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8101102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCS2"/>
    <dbReference type="OMA" id="IYISKHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="468" mass="55238" checksum="3E00BF9000772EC5" modified="2022-10-12" version="1">MNNSDQQKEQIFKQFIKIYSNRFQQKEFQHTLFSSQILIISQEIIQQEQLLQTQIVSLDQIKEQREQLLQKLDQKESELSRIEKLIESNNKREQNQHQFEDLQDGILMCKEKCVMLQEENLEFNKMAGSLRQQNDQLKIRYSQARLNYDNNLSTLNELQQYGLNLNYRIMKQIKIIKDLGQLNHCGINQNRKSKLENFHLEELYNNLELQEQNIDSEVGESIQQLISIKRELAQINMKDSSQNSPRQQDLQKVDPILTSGAFRFKTHHNLGEKQIRQRQISQQEDDLRAFSELNEEDEVKQSFSDEDDKHPEQISHKQPWYFKSAVPIISSKNSEKKWENYSEKKKHHKNKYSFASASSNSSFNRMGVMTLKDEFQSLYQQQQVDEDIKEKDTEMNDSDEIPQLIQQQSILQDSTIIINRKSQQLSKSKVQIETIEQPVKSDSYKNLKIAGVLLATCGIAGWLYKKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCT2</accession>
    <name>A0A8S1PCT2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1130104</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCT2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8100799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="86" mass="10180" checksum="A0291681C819D437" modified="2022-10-12" version="1">MIKERIVEPIQQQLNIQLEEIKETQQLQEIQQPILQKPIDIEKRVEAVILQMDGDGNSNSRFKFTNSKNVPQDYEAIQQIKQYLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PEU4</accession>
    <name>A0A8S1PEU4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1160072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PEU4"/>
    <dbReference type="OMA" id="YYQITVT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="120"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8101666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="277" mass="33337" checksum="D2AD489FE5AD3EE8" modified="2022-10-12" version="1">MLVKRYRVQLNHLFQNDNKVYYQITVTKVNNENETKTTLNRYSQLKDFHDKLHKNVTLLKLQMQLPQFPGRSLFSKTNQNEEKIIKRKIELENYFNELFSIDKILSLTPVQQYLPIEILQNQQMNISIRIENYVIYDEVVVYILRFKNNLLNDEWIYKKRYSEIKSIHDALLDQGFKNKLPQFPTRKLFGQTNENPETIEKRKEDLENYLNALFCTQEIQESQIIQFLIQDSKKYHEKNLKLEELKKSSTLKAKGGDLNKRIDEFPQRKNQQINENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJV0</accession>
    <name>A0A8S1PJV0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1210035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJV0"/>
    <dbReference type="OMA" id="HYYVIAG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="171" mass="20043" checksum="F813A456A89BCEBE" modified="2022-10-12" version="1">MYHYFVVVGGMILGLIVLAVLNKNESQKKKVKIDDQDEQTKLKEQNQYEKQESNKFEPQNINDDMEALRQRKIESDERKNKIFKNLSVEDRQLVEEYFKKQAKQYISGEVNPADRRAAKLIQIIKFSLYVLFGLAVYISLTIAFNTTSPLMIIENLKHLVRTFLSQLFAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLH9</accession>
    <name>A0A8S1PLH9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1230067</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLH9"/>
    <dbReference type="OMA" id="RMQQRND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="370"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="742"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8104220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="873" mass="101697" checksum="67AF91B851C19AA5" modified="2022-10-12" version="1">MKFNNKEFWYYFKCIIDIALSAIIYGYSKDSFIVTINTVTIQLLGILLSPCIAKKFGRKKRKYIQIIMLLAQLAQIFYCCFYFTEMLRYGYLLIKFYEKQLIDNLQKQFSYIFFIALNALQLFLMISQNEQGVTYFTVGYATMLIFKEKHQNMKKKEKQKIKFKEFEKTDIKPSRYQTRQSLLINRISESVSMKWMNKLQTFPVGIVIVKKENLEILFENDQLLQIFEAESDIGKFILENIECTIIQKKNNYFMHQTSIVLEKNGMIQASGTFNLTQQKIQNKTFTIKQILNLLNMGHLEKFKTRDDQLELSATFCKGNEAFFEERNLLFKISSAQDENEFLIIIQDITLQTNFKKMETKEQFIVKMIDSFSHELRTPLNSAELFLQALSQTNSLPVQLKENFIDPAKNSLRLQAYIIRDIIDFTQFNQKMIKYDFSDFNFVDIIQEINDLFKLIFQHKKIGLHVNAQRSIPTVIKSDYYRIMQILVNILQNSVKFSSKGQVEVVIQGFDGYVLFSIQDQGQGIEPQLLTKIQQTLQDFALNRSLSQQHEWQGLGLLVSQMNLITLAPQNKSYLQITSKGLNQGVEVKFRIKTQTQVFTQQLAQHKTFKRGSLQTPNTVPDLSEGLQGLLIITNAEYKNASVKNIPVKDLPSIADYYGKPQSNQIVGLQSQLETDLQIQDSDRFGLTHQLIQIQSNFYPKFLTTKHRQNSLVSRMQQRNDDQSQLQINFNKVKFKFKCQCQRALSVDDDSFNQKALEMILGQMGFDIQLAYNGQQAIEIIKNTKKCSENCQLYIFILMDCQMPILDGWQTTKRLRNMMTGNIIPSIPIIGLTAFNGQYEIEKCYESGMQIVLTKPLNIDDFKQALIRIYRFVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PP07</accession>
    <name>A0A8S1PP07_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COMM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1260104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PP07"/>
    <dbReference type="OMA" id="EVIHDQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="1">
        <location>
            <begin position="123"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="194" mass="23001" checksum="240FEF3314E7962C" modified="2022-10-12" version="1">MQNYVYDIDSIRGLNLSLKVPLSLFAYMVDEIYKFTLSYQSAFSLQSMLQIFQRESVALDISESEFCINSYVYLIRFVQKNNITAEHLSKWLKENTLVQDDHREYLMKVLQGFDPETVKKVLSLGKLKELKWTVNQYNGDVKYPNLKKLYILLEFHIQDEKGNLQKQIINLTIQEFKMLQMEFKEIGDILRSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQS9</accession>
    <name>A0A8S1PQS9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1270111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQS9"/>
    <dbReference type="OMA" id="KTAGVYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="1">
        <location>
            <begin position="129"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="252" mass="29362" checksum="AB34F0055C02BCB0" modified="2022-10-12" version="1">MQQKLSRAQLIGHYRQKPIEQMPESFKALCQQYPQAFKALSQTPYQTRPIRGFNQEILVYSSNCATPLKTAGVYQSGGGDLFRFDEQQILTPQPKQSNTFDWSQQQLQSIKKEIVFSRSIPGQVNYLLQKIWYYVDDYLKVQGPFSSIQMDNWYLKGYFDDILRIGFKPENSESFIRIYQLKTQKQSKKVLKLLRRGSCPPSITASQEATIQLKPTVQQQQQQQKQDIQQQIDTQNIDNAPTIRVPTLRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PV44</accession>
    <name>A0A8S1PV44_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1320048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8106904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PV44"/>
    <dbReference type="OMA" id="KFGKEYH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="592"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="592"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="609"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8106904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="634" mass="74145" checksum="6D4E4691084D5C9F" modified="2022-10-12" version="1">MLQQYNSLVVDASFINKNFVQVSVIGKGHDFYLGISSISYLMHHKKEQGRYILQIYENLGYDLFRTKVMQLQKAMLECVNDVNTRIILLAEKQLNDKQSDLYVLYQLNELTQRLDQQKQLTSEQAQELLITFCKNLLITGRVTYEFLDLAPQNIYVNGDRFVVSNMGISYKGSKFHRPYTPITDSFYKEKTLTNMIYTYSFQAGLVILCAVTKINSAEFFFEDGQLKNNLLQKVLKVFREKLPDLKKQKLKKKFNSLAKIEKPFDTDISNIIHFLSLILSMDQQKRSIFSLLICHPENPLKVENPFFQEPIDVEQQYIGFGKLDGGRIEFEGYGIAKFGKEYHYGIFKNGTLTDDGTVEIKYNNSIIYLLKNLVISQKYQNAIAEVNKYIFWGEFDNKQYKPNGEGILVNVFTTQKLLPKKRQSMYFEGTMKMGIKDDGQEFYKNKTEFSGKYKDNQPNDGSITYGPKHKFYGNIENFQRTKGILIYKNKQFEGEFENDKVKIGKLSYPDGSRYEGQFQDGLRCCQDGKFIYWNQKLEYCGGFLDDRFHGKGILTQFQTKEQLEVEYNYGKCLTELPESFKQLMEKKQIYELKQQDNQTKENKQKQNNDEDIEEDIQEEGLGDEKQDDYYEDEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PVW2</accession>
    <name>A0A8S1PVW2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1330142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PVW2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="98" mass="12135" checksum="A52FE7B55F1FFCC1" modified="2022-10-12" version="1">MTTIRQYFIQRLQTYKNQLYYKQIAYLRPWQNHLELQKVEFTFVKQMQFLILQSQVINQQRKKSQNQQTFYKFKSLITSHRKRLIDSFQRAEIATNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PWV6</accession>
    <name>A0A8S1PWV6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1340036</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="RDELTQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000335">
        <property type="entry name" value="ATP binding cassette subfamily A member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000298">
        <property type="entry name" value="ATP-binding cassette sub-family A member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF36">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="333"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="467"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1087"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1147"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1173"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1202"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1239"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1284"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="551"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1348"/>
            <end position="1585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8107541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1758" mass="201240" checksum="A4946F802419EEE8" modified="2022-10-12" version="1">MGHLSSLLRKNWILWKRNCFCSTCEIILPLILILALGGIRGVVDKTNLDETAFLQPELDWKSSDPNVPVMLYSMINEKEIEDKIMIIQKWNASLNLVDNLKSLDTKSRLSVLPKMKNCIDNYNYQQERFWLNGHVALGPNENHEIVKELKYIFETYYNYQTIIFKNNDELNEYTADSDYGRDGVPRICFGVMFNETAGNNIYDYQLRFNTSGFNDFEIPPTNLMEVDPIKYQDYEKVNTYWESGMLTVQTFIDNIILRIETNSQATINPKFSYVHQQEGVKDEFATFLRGQFGVYLILPLIIIYLRMTYAMIYEKEKKLREGMKMMGLNNTSFYLSWIIQYLLIYTIISIIATILLSAIVFTHTDGFVLFLNYWLFCIVLIFQSMFISVFFTRALFGLIVAIVWYLLMYMVISLVGSGQEIVPESSYWGASISSHAGMSFAFDVMVMFEAQGRGVSMSTISTKVENYAVNIALIMHILNIFFYLIMSIYLDLVFPNEWGKKLHPLFCIPYFNRSHHSQQQRLRKKSSLIHQERYEEVEQALKDQESRKEVLQISNLTKIYPSGKQAVSNVSLTMYVGQIYALLGHNGAGKTTTISMLTGLLDITEGEATVFGYDVETQIEEIRQFMGVCPQHDILFDNMTVKEHLEMFATFKGMKPEDIPAAVRRMIEDVDLLEKTDYLSKNLSGGQKRRLSVAIAFIGNSKLIYLDEPTSGMDTSARRYIWEMLKNYKEDRIIVLTTHFMDEADFLGDRIGIMGEGKLQCSGSSVFLKNQFGNGYNLTIVKESTLTESDPIIEVIMKVCPEAILISKVSAEILMQLPLNAVIKFPKLFAELDNNLKTLHIQSYGISITTLEEVFLKVAQIGAGHHQVNDYMEMEDKNKQAIQIDDFDINQIRVTNSTQLFFNHTIALLMKRARFFKRDLKSLCCEILLPCLVVLLGLILMTIEFITEPNIIILTPPSECYGNGIQYLWGGLNDQSLFSQINLEMYNSSIQVFGDDSLINLQNMDQNYFDTFDLRSNIGWYYLTSNSNNQYSYYMFVNTIFREAPIVLQNQMNQAILRKATNNNNYQIKVTNSPLRKTYEELNGSKAIAGFLSALVFSMGMAFIPASLISYIVKEREINIKHQQLVSGVSVKAYWFSNWLMDLGKHVIPAVVCCLLILAFDISALIEGENYGFSWLIFFLYGWAIIPFCYFFSFAFKQQGNAMLLSFFIHLLVGSIISLIIYILRLISSTRDVATILQWIFRLIPSFSFAYGILNACSKDIYMIMEGWTEMKSTYDMEVSGGDVLMLAIMGAFYLICIFIIEYFEDNGQLQKFGSSESSIPYIPKPIDDDVAKEKQLCETFRPNEKAILVKELRKVFMLGEGKHKVAVDQVSFAIDQGEVFGLLGVNGAGKTTTFKILSGELKPTSGEAFIAGKSVINELEAARVNIGYCPQFDALLDNLTVREHIELFSDIKGIPYQRKEELVEKKLNEMDLKRFEHIQSGQLSGGNKRKLSVAIAMIGNPPIVFLDEPSTGMDPEARRFMWNVISRIATQRKQSTIILTTHSMEEAEALSTKIAIQVSGNLRCLGSVQHIKSKFGKGYEIEVKLEKPQKNEIQLLIQQMGLENNSRLDQNTTVDILRKINSSHLEQQIIMKGSGSHIYNDIRKPNRLAVETLAEYVIVEKMGDLLQKFIQQSFGQFEIIEHFQTFYRFRLLGQITVSKLFEGFEQNKKQNRISQYSIKQASIEQIFNNFALQDSQQQQAHEEIKGNQISVQIQQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PWW5</accession>
    <name>A0A8S1PWW5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1330042</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PWW5"/>
    <dbReference type="OMA" id="IHYDAYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053238">
        <property type="entry name" value="RING-H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155:SF627">
        <property type="entry name" value="OS06G0192800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="268"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8107188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="400" mass="47770" checksum="AF7913B7CD434377" modified="2022-10-12" version="1">MLATQINHIINTLISQSQSKINLENVHLFVFNKCLCKSGYFGKYCQLQRKIAVLQIQYDIIIKPLQWIFIGFETKQLNSNDTLKLRIRDQSSEMLMGLGMQGIENHEVPNIHYDAYIIPKKIQQQIYQLKGDLIYYQSMCMNCTFLNIGFYNQHSYPILFYFELFEKEQPKDNDPYHLQIILQSIFITCFILITYLIFRKIKQKQQREQIRLVQREFRGNRRRRRIQQQQSDDIQKGFNLDFIQTYFPQFYYLDLIDKYPQLSQFVECVVCLESMAPPQQGKLTERDHIDHCSLTPCYHLFHQHCLFKWLQTQKCCPLCRKEFIEQDIKNKPWISFNNDISLRETSRANPFVQRMKTRSENTQQSDIPLMVDTENVETQPNKDIVVVNIAQKSARDLIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PX30</accession>
    <name>A0A8S1PX30_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1350003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PX30"/>
    <dbReference type="OMA" id="SHWNITI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="65323" checksum="0FB5195BACFCC947" modified="2022-10-12" version="1">MFTLQSKENLQDYQCTFKHNQPVSLVSLQENLLPNERLYCKQCIDYIEHDLKPLQGVIKIIEEPLKQILKQYENDLIPQLKKLKNINGILQQLRRTIMQELDDLENRVNDFTNSLINIGKQMANYSFFDCLNNLIINQGELHIKPIDLIKINDEWCWEMIRGLDRFQSYEENKDIQIILNQIMKNQLFFVKYKDARKTPENIFQLSPNTINNNNKQSTFEIISKFQLKKKVDCYALAINHDNSLFLVGLDEGIKVMEIQSDMQQQKYNTNQVITLKDVGIYKVQNLVFFKKKTAMLNSFISSSDGCLIIIWSQATNFFNKSKWNQIFKLQQKPLQNWLIRNYDIKFLQLNSEENQITFALNSQIHFLSSSKSWACEQKIQNSLDEIRGLSINPNGNQLVACDLQNITIRELHNSEWKLKQTLPIIGQNFCFINNFMFSFQPIQSQALSIYSFDCNIREYIKSSEIICKGLVKQDKIIESYNSTYVSSKNLLLSINGNFINILQFSISQNLQNYECRVAQSIEFKDISIIGTISDNGEFLIIYDPTLNEIQIRENQDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZU5</accession>
    <name>A0A8S1PZU5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T1360044</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX41 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZU5"/>
    <dbReference type="OMA" id="FKTIWTL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17951">
        <property type="entry name" value="DEADc_DDX41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000657">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DDX41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044113">
        <property type="entry name" value="DEADc_DDX41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="17">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="142"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="337"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="533"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8108169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="560" mass="64345" checksum="E0BEC37C71340D8E" modified="2022-10-12" version="1">MHQQQQQQDEYKRSLVQEKMQMKQQGIDNGNMRKINDEDIQKIQAYEQMQIENAMKNSTWSTLMLLKSKEIVKSKWRPKKKQRLWDQYKIDKILKKYSIMIEGNDPPPPIKSFQDLRVDNRILKILSKMKIKKPTPIQMQGLPAVLMGRDIIGVAPSGQGKTLVFLLPALLQCIEEEMKMPIIRGEGPFALILLPSHELAILTYELAKQYCQKFQKKGFPAIHCLLGIGGMDMSSQLQSIRNGVHIVIGTPGRISDMVNKKKINMDLCRFIVLDEADRMLDQVFELEIRNILEHFTGPRQTMLFSATLPKKIQEFTKQTLVDPLVINVGRSGQINLNVIQEILYVKQEEKLHYLLDCLKKTTPPVVIFSEHQNDVDDINEYLLIKGVEVVGLHGGKQQEDRTKALKQFLNGQKDVLVATDVAAKGLDFPDIKHVINYDMPKDIESYIHRIGRTGRQGKTGRATTFVNRQQEESILLDLKYLLVESKQKIPQFLEKLKSDEDLNGSCGYCDGMGHRMANCPKLEKQKMRILTNPNKESCPDFLKGQDYRESQSAERQNQML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZZ2</accession>
    <name>A0A8S1PZZ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1360069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CQALEKT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31513">
        <property type="entry name" value="EPHRIN TYPE-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31513:SF2">
        <property type="entry name" value="MRAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="705"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="831"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1160"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1199"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1236"/>
            <end position="1261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8108219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1290" mass="145388" checksum="7995D746188E9B6C" modified="2022-10-12" version="1">MNNDLELTSNFKIVSQENVTIDSKSIITASTIIIYSNHTIVDGIIQSPFDCYTNQGVGKAPIFTIEGLICLSGGGSSAGLGGRGSQDKQCQALEKTSKQFIKSHPYNHPNYLRSGSGGAGTISIGSGGGMILLQSLNTISINGELLCNGVDGQFINLQNQFGGGGGGAIQILAKYIDGNGVIQAKGGNSDVSNQVGEGGGGIVLVQGELSKMAKLKIDVRSGIRKDQQAQNGTIQFLPCEDGYHLNKFQCVECPSNSISFAKRGHCFKXILIKSSLIYQNHAKFNFVCIKNHLIMNVKQLYFTQSNITNCEIIKIDSEYIEIHNINLINNNTSQYQFQSQGNILVQGNIEQIFNHTELIIESQNDITIKDAQINIDQLAIVSHNGLIEITNSNVISLNPKCNMNNDLELTSNFKIVSQENVTIDSKSIITASTIIIYSNHTIVDGIIQSPFDCYTNQGVGKAPIFTIEGLICLSGGGSSAGLGGRGSQDKQCQALEKTSKQFIKSHPYNHPNYLRSGSGGAGTISIGSGGGMILLQSLNTISINGELLCNGVDGQFINLQNQFGGGGGGAIQILAKYIDGNGVIQAKGGNSDVSNQVGEGGGGIVLVQGELSKMAKLKIDVRSGIRKDQQAQNGTIQFLPCEDGYHLNKFQCVECPSNSISFAKRGHCFKCPGVLFGQYYVDWPSCNEKVCNQFYCDPITFISNIMFSLLNLILYLILLLFISIGYVIRRMYRMQEIKYEKLPFDFESATRYMTIDHLLTDELMKNNNFQLIDLMYHVHRITLLGNNSPENPWKLPSHPESGIEYLMDLEEYKRFANKINKFAEWKKQEKILLIIISILYYPLYWMILTYIKKKKYLRIIEYFHQSNIMKLMDDQFIKIKISKSADYTLLYFDIFNYKISDLKYFFLACPYYILVSGEGNFLKPFYLCESNPFLSCLYFSINRNKEIHEDRNKILDNLVTHKKRMENSQKLNNFIIKFNRYARAIDVDSNDFAQNVQKLIIFCNHQNVKFFSDFLITINIIIIIDDEFTILENYESQEFIKFIEEAKNKHLRFGLFIYDSEANPEEIEDVLDNQRISLEQSNQFQVLEEDKESFDNVQNLEDLKHQYIERIQDSVQSSQEIQSEKQNKSRNRKSTMEIFNKIRYLFLSHQVPTVGQNTKIYVQLLLSILDAIFLPLLIQQGIDASDFYFEQIMLLSFPYPLSIIISPILGCVWMTTQSYGIAKVHSIFNSLSIFNLIVHIIIMIQKMIPLAFVILVINLILKILLMINIKRILSLNIKLNKLNQIKHIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1A1</accession>
    <name>A0A8S1Q1A1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1390030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8108991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1A1"/>
    <dbReference type="OMA" id="QRTVNHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="481" mass="56422" checksum="D2EFB7999F12165A" modified="2022-10-12" version="1">MQSDFQEEVVKSEYVQAEDQSFKFVNMPSYNPEQLVEDRLRESIRQSAAQRTVNHQDDPTSVKFHRHQSYNQLQAWMEQVGIDMQQHRQIIKDVFRILEKRIAISNRSIQNLIHFIKTFVQHEKQNVEYLRTKNNAINKLFQDGQTLYYPKLDETIKEICNYDQGNAIKILNLLDGIDKFIKEKLDFNIEMFETGISIHRDQYRKAFSNFASSSSKLTKYRRKHQGLYLRQMQGESKGKDLYHTERKLINSFLEMAKLQKNMGQSVTKLFDEVKKQEVHRYQIVITSLRMYLDKHVQLSQSKQQQISAQEELIYGLINQLEAEQVVQSFEVLNFLGQDIVKIIQKMLGKQSLTLPQLQEFLCNYPDTYIKLGSVQENHSLVKLRIKNAQIDCGNVLKTWKPCQIIATIDNNILIYLKENNEFKGKFLCDSSQIVQKTRVPLTAEIKYTKPGFIFDSTKTALLQLEAQDFDALHGLLFKQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1F9</accession>
    <name>A0A8S1Q1F9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1400131</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1F9"/>
    <dbReference type="OMA" id="IEMETQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045194">
        <property type="entry name" value="MGRN1/RNF157-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22996">
        <property type="entry name" value="MAHOGUNIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22996:SF0">
        <property type="entry name" value="RE60872P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="235"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8109414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="287" mass="34123" checksum="AE0D2B5AC4BB6E80" modified="2022-10-12" version="1">MGNNQNNNNNTANHQSVQHLTNDPNYQQVLRDLYQNNPQQIIQDFPQYLQNQDQHPARFKHNVEVKKVVPKQNNTYIKKDSFKLVYIEYIQYQTQIDASTYQIEFVFECSEPMTLKIHLLAVETINNEFITQKINAFQQKTYNFEPVSGYKFDQFQFDIRQIKLEDLEYTNQEKRQYPLIIEMETQEKQLFQYCFFKLNQNEIQLQTFEIKMQKNGKAFSVRDVYGGQKDQDKDCVICLSNKVNTLILPCKHMSLCQTCCQGLKERSSKCPICRNRISSFRIIMRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1G6</accession>
    <name>A0A8S1Q1G6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1390066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8109061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1G6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="119"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="250" mass="29939" checksum="77892787BD388FAC" modified="2022-10-12" version="1">MILKHYSVKINNLIQNDKVHYQIIVTNVNNTSDTKTTMNRYSELKDFNEQLIKNINLLKLQLQLPEFPKRSLFSKTNKNQEKIIQRQQELEQYFNQLFSIDKILSLPPVQSYLPIETPINQQMKISVSIESYTVYDDVVIYSMRFKNRITKEEWIYKQRYSEIKNIHDALVEQGYKGKLPPFPTRKLFGQTNENPETIEKRREDLEVYFNAIFSTQEIYDNEIIQFLISDSKKYFETNKKLEEQKKIQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4N3</accession>
    <name>A0A8S1Q4N3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1450005</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1450006</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q4N3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8110628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="67" mass="7932" checksum="5ECFA3C058694099" modified="2022-10-12" version="1">MNQPGKGIILREQSFLGLFRLTTQKKCKKQIDFHFNKLISSIISIYYAVLYYDYKSQKDISINNRVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5I9</accession>
    <name>A0A8S1Q5I9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1430114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000147">
        <property type="protein sequence ID" value="CAD8110295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5I9"/>
    <dbReference type="OMA" id="MHDIHVS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000181">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000084">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="141"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="452" mass="53440" checksum="A1D287BF883D523A" modified="2022-10-12" version="1">MIGYQNQTSEDQDIFGSQAFEIIDKVQRKPSQRESFSKKQSFVNKQAKRSISAIKSDENKMMINNGQALKRSKDDHPLTGQEQLEFKLIQVEISDFIIIEHSENGKDYKILDQNDWKKIIKQKSLSGYMMHDIHVSLLTQNLFFIERRDIWLLFCQIDSIKQKINQKNSTFFQYSTQDNNYSQQIDKDIPRALMGNRFLNLQENQISLKRILMAYANYDPELGYTQGMNIIAANLLICYDLKTNDEKFLEDVEIIDPSRDENVFYLFIYIMVELNWRSVFIPGFPGLIRMMKVLDLKFQNELPQLYAHFQESNVDLNVCFQQQYLSLLMYGISWEISRMIFDLFLFEGEVIIHTFLIGMLKYCQDNLLRLKTFEEITKFCKSEMIRQFYELFSIKFVGNKDFSTILLNLGFIKVQGQIVPPQPVSPVKQVKQNNKFEIWKGTFLKNFFQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QAU8</accession>
    <name>A0A8S1QAU8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1520003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035771851" evidence="2">
        <location>
            <begin position="21"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8112513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="258" mass="30037" checksum="281306610FE3DB90" modified="2022-10-12" version="1">MLQFNSIQLLLLVLAVTTYSNEVFETNTMIPISVIAQIRNTEFKDQTFQNQLLDNQNEVSDDKNEVNNLFLIEPSKKSLKVFSQKSLLVENQPEEVQKESSQQNEQILSQEQKDQQNKISIKVNKKLMKDTEISQENNIEEQHILEKEELQPIQNDKIPIKISQEKQEIENNDENEITIQDIKIESIHKEPISKIEENKSQNEDVIIIKIDEEIKATPRIFGILDNDSIKQEXLLKLGLTEIQGSLIYMINFNHKRIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QHI2</accession>
    <name>A0A8S1QHI2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1620090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8115063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QHI2"/>
    <dbReference type="OMA" id="DWRIHIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8115063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="388" mass="45446" checksum="76424D779EB75E54" modified="2022-10-12" version="1">MQDPVLQCLQENHYSKQIIGVCVNSECQKIRPYCQQCLKCYLHSEHLHDLQDFTDFYNYVQEGLNTYNKMQNLAEELQSIAKLITIIINQLSFNPKINLKDMSIIAINNLINKIIRVKEVEKTMKNNLELAMIPLQKIKQDFQELIHKNNEQQLIREEPHKKIPQKSKKQRRAVKECSKGLQIIQHQIMTKQEEIKHQVVCIKPSKSKDEKKQQQQQQMIINQGKYQFSNLYKNRAINVIQPENSAEGFGFCVCDQAISKIGITLIAFRIIKCDWRIHIGVCDRDILIESKYDPNLANLDHGTYLINNQGDVFSTADIENNDKSKSFKFYNKNIITIEIDYQLQQITWIKKQPNETFTLKFDTQKELYPCVKLWGSSKVELLNPIILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJ96</accession>
    <name>A0A8S1QJ96_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein disulfide-isomerase</fullName>
            <ecNumber evidence="4">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1630057</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000170">
        <property type="protein sequence ID" value="CAD8115244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJ96"/>
    <dbReference type="OMA" id="AYYWVAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF132">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035864381" description="protein disulfide-isomerase" evidence="8">
        <location>
            <begin position="21"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8115244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="512" mass="60419" checksum="440D60CB31B1B022" modified="2022-10-12" version="1">MKAQHLMLVCLLIMFASAQLAPIKTEEEIKIDLSLEEKLEIKGSNDIKPNQIFNKLYEVDKEAYDYLKKSFKNVVHLVFEQNEEESQVKLLNSIIENVNFTKIDVIFVKSPIGLIELNKGIIVKNFDKIFRIHNFENLQSQIFDIINPKIEFLQTEDDVNKYFSQNQLNSLVLLPDNYTFRDQIIEDLETLIRNGDLKEARIDGLKGYNSNYKNYTAPTLILHKGNDKFEQYEYNLTYTDQILDFFYLESLDLVNRLDEDNYARVFGGPIQTQVHLLIKGIFKQDLFNAYYWVAQNNKMNDLKYHRLIFTYSTFDENPNLWSLFDFDQTTKTPQLVITETNFRKYQLQKYICQSETLNQETIQQFIDDFRNKNLTEFYKSEPIPEQIENQDIYKIVGQTFSQEVLNKGKNVLILFWDSKNKELFDDYFKLLNEFSLIQNKEKIKIGHIDLANNEHPKITVDSVPKLMLYLAADKKAPKEYLKSSNLVIQELKAWLDYYIGDGIAYKQEKTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QK13</accession>
    <name>A0A8S1QK13_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA processing factor 4 (PRP4)-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1660030</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000175">
        <property type="protein sequence ID" value="CAD8115766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QK13"/>
    <dbReference type="OMA" id="LNEPICY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.110:FF:000015">
        <property type="entry name" value="WD40-repeat-containing domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014906">
        <property type="entry name" value="PRP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846:SF0">
        <property type="entry name" value="PRE-MRNA PROCESSING FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08799">
        <property type="entry name" value="PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00500">
        <property type="entry name" value="SFM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Pre-mRNA processing factor 4 (PRP4)-like" evidence="3">
        <location>
            <begin position="73"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="199"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="302"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="386"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="428"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8115766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="503" mass="57614" checksum="496B1DE7814E593E" modified="2022-10-12" version="1">MNLQQQERQRVTDNLSQISQLSTRAPTIIPQQQESLPFISYTTENVDRQQKQQEENERKQKLMAEARKLMVPTQESEIKSQLRQLGQPVTYFGESAVDRRQRLRTLLTEHLMQYGRLPQFFKQQNNHNMGNLENEYFLYEGIEQLRAARIEIAKLSLHNAALRVEKQRLRRLTVDALLEDQEVLDEMQKVNHFAIQMSQFGDSSGISRSTISPNQKYLATAGGSGECKLWDIENASLKSSFLGHLTKCHSIAFHPQSLLTLSSQSFANVATASADLSIRLWTLDLDQVEVENLGMLQKSIVLKGHEDRINKVIFHQDGKRLISMGFDKTWRLWDIETQTELMVQTGHSRAIYSGTLHPDGSLLFTGDLGGFGMAWDLRIGKGILPFVGHVKGILASDFSMNGYHIATGGDDNFIRVWDIRRRNVIEKIPAHIKLVSDLKFQPIYSKFLISASYDGNIKFWNSRDWSLHSVYEIQDTKHTSVCITDDIKTMITTGLDRKFVIWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKH9</accession>
    <name>A0A8S1QKH9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1630012</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000170">
        <property type="protein sequence ID" value="CAD8115170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKH9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045862">
        <property type="term" value="P:positive regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="61"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="8">
        <location>
            <begin position="243"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="74"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8115170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="331" mass="39212" checksum="A2FD56B9C7E36452" modified="2022-10-12" version="1">MGNRASNHEEISAFLSEFEYQQKNFSIQYDYPQRKVFKAAKKRNQISHSSNRRCSQNIKKSLEYKVEGNHYIQLKQYKKAIESYTQAINLYDRDSIYFSNRSVANKLLNRFQEAKQDAEKALKIDEHNSRAHFIYGTIILIEVQMFPDTTESLIKQAQLGIKELESAQDQVKISKNEQKNKLRVLINQNLAKGKRMIYLIRQEIDKRNIQSLKQTLLDISHRQQLQLDWNLIEQNIKRKIEIQMPEYFVCPILFEIMEEPILLNSGLSYDKSSIQQQFRQNGYIDPLTRQSIDPLSLIENIQLRNGIIEIKKEYGWIGIEHEKDYKAIRFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPB0</accession>
    <name>A0A8S1QPB0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1880010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000197">
        <property type="protein sequence ID" value="CAD8117101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPB0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="124" mass="13725" checksum="8DA09089ADA5D3AA" modified="2022-10-12" version="1">MKCSSNINEQSCTYALLQFQSTQYQLCRWNNSLGPQGVCENAYSALLQTSQICLNKTGNIFRWITNNESAGKCVSYGAIQLSFNCLRSGTSNCQELNSCSLLTITNQSEMSLVSCAQNKNQIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN87</accession>
    <name>A0A8S1JN87_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0050012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JN87"/>
    <dbReference type="OMA" id="DCTIGTT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="130"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="392"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="566" mass="67423" checksum="76A90B193887EE0D" modified="2022-10-12" version="1">MRLNNIMKLALTVSLLIIFMLTAYYYWNNSEDYVSNIFPPQVDNDLSFFSQSVCQRVKNTELFQGWKKINEQALQLNERYSTELLKKAAFVIEQLKKNQQIYTQSQGEGQQFQYYQKNLKECVNRTKISKLELEEIKNKTNQILEQENSEIKKINENISVHQNGLKDLNNLHSEKRIQLNIVIETLKGLIDQKERLRRSYKNEKELKDYQKELEHDINKISDIMSQQELNITIFKSRIEEQESKVQKITKRFEVVNNQLQDLKSLEQYFRQDDLYIKEDVPIVQVFSEYSNTLKDRAENLKILAQKEKDPIQRQKNLEALTEIQKALQISSQMYNSLLEIKLECNQDDCTIGTTLENLKLSDLKEEIQKKQTYYNQIYTEKQSMEFSLQQLMQQLDNENSAYMINKQKLNKLIENQNLLNKTKNQSEELSKLTIKINQLEQEKNQLQEEVEKLLNQANDQQHQIDAEFEKVNDKKKRVETLQVDRNLSINEKHQELYKQVKECIKQKKIYDNFVQKQSNLIEKANEIKAVVQTEVRQIESMLNEYKITYQDLNLQSNNDYEKKQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNP2</accession>
    <name>A0A8S1JNP2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0040218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNP2"/>
    <dbReference type="OMA" id="TLVHTEE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000184">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="163"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8042999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="351" mass="41287" checksum="3E8D155CC103B382" modified="2022-10-12" version="1">MNTKTLIFQRSTINSEQWKLLQRDPQLFKLAQQLNRNRTQSNITTSKQMKIGNQSISRIKLDSIKIDSNDDTLQQNEEKSKQKMIIASRSGQFIRKLNLNNQVYIKKVYQGNYFFDKIKEILTSGLKDNCPLKKMSRDALNQIHMIMTYQESIPEVSLLYQGKSQKQIKIVFDLDETLVHTEEVQKDKVYDFQNNEFGLFIRPYCRHVLKELSQLADLFVFTSANQKYAKTIINLIDPENTYFKGHFYRNHCISLQSRMQLKHLGILSNNYSKTVIIDNSPIFYMGQPYNGIPIAPFIDDAQDNELMKLLNFLIEKIIQSDDVRVTIKRYFQYDMFRQFSDGVTAFNILYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP58</accession>
    <name>A0A8S1JP58_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JP58"/>
    <dbReference type="OMA" id="ACSFVTM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="147" mass="16707" checksum="DF68F67613E91E8B" modified="2022-10-12" version="1">MQRLSSLLIGFGLGIFGMSLLYDPWAQERFERTVRETARLENSILNNVDVYLQMRMFLGYILVACSFVTMNRIGLLYVLSIPALIVYAAINYNHVIYKSERLGGEQAIQGALFYIAFCFASIDGLIKPKKIQQPKTAQKKVDKTKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQ78</accession>
    <name>A0A8S1JQ78_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQ78"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="81"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="16407" checksum="6C125AC92E55F849" modified="2022-10-12" version="1">MLQTYINRFLIKFGIIKKDESFIVREEIRENETWSDLRQNRIKQIKDDLESGKLMLIKRSLNNQDELYLYRVFQCKLIFYEGISSVHLAASLGRLKMLQDLKLYGAQFDAKDELGMTPLDRAILYNHSEIIDFLKQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQQ4</accession>
    <name>A0A8S1JQQ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0040118</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8042900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="998" mass="114880" checksum="970651DD5C4FD33A" modified="2022-10-12" version="1" fragment="single">MYSELIISENINHTFEYNSQYKLIKQFQRNFLKGENIHYSFWNGYPIFGGSYIWAQAQFERILLINRPHHSISIAFYILYGPEFPSDSKFICSINENILIIKNKNDAKQQNDGTFVDVISTKINHYTNTLNITIECQGENNEPIKAFCGLYNFYLAVHYCQPYCNKCTDHLTCQEWNSTDYITQIQFSQVDCNTTQYFNVHQSLCFDCPSSCKRCNSKFECLECIDSFKLTNLGCFCTKSQYEESNRCNDCQTSCNQCLSYSQCIECTDSVNMVLSNGQCNCIDGYSLNSDSKLCISQGSITLIECKPECNNCVLGKCYECDYRWNVDPFEYICKEKCGDFIQLVSEQCESSFILPYKGCYNCKPRCQSSCLNCSTTGQGCLECVEGYSLIDNLCYSICGDLIVTYDEQCDDGNLIYGDGCHFCQYSCQDSCQDCINGICYNFNLNHPLTIIQCNPLLGNEIYNNSQLCNLIGELYQLSEQYNYNFACDINCQSCFFQICYICNENYMLSIDSRQCIQITLLFDYQLEHCLINVNNYCQQCNIYAYYDKIEQKCKPNLQHLKQCQYQMIQSPKQYCSQCFENCIQCSYDGCIECKKGYYFDDQLKCISNCGDGILTDNEQCEINDQNCLSCQFQNSQYCLDYHQELCLQCQLGFYYNLVTKMCTSICGDGIKAEDEDCDIVNQNINVGCQQCKYYCNNDCNLCKAGQCISCNQGYILLNNFCYINDLRIENNLIIEQLDIFKFETYCLTNLGYYLPLVFNQCIPICGDGIITQDEECDDKLTYQSHICFNCKFQCPINCLQCLYGQCIQCIEGFSIQENRCYPKCRDQLIKSDKICEDDNNIQFDGCYKCQNSCQIECLVCQNTKCVQCISGWNLIQGKCNQICGDGQVAIISEEQCDDPQNLNCRNCQIFICNEYCKLCDNQQQCIICNDTFNLVNNKCINICGDGLLTQGFEECDDGNQIEFDGCYNCKYSCSKGCLVCQENNICLQCNNEEQFXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQQ6</accession>
    <name>A0A8S1JQQ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0080462</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQQ6"/>
    <dbReference type="OMA" id="DWSWEYA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF18">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="10"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8044893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="327" mass="36302" checksum="84B56074242C11DD" modified="2022-10-12" version="1">MKHVTQQEYGSPQVLKLGESDIPKPLENQSLIEIKSTSINRADTLQRQGLYAIPKGSTSILGLEFAGYELDENGNRIRKVMSILPGGGYSQFVVVNKDHLIDVPANIPLEIAGGICEVYLTAYLLFKLSDLRKGDSCLIYAAASGVGQAALQLSNIFGINAIASCSKGKVDAIKKYTKNIIIRDESIDEQFKQLQTLFPQGVNAIFDCIGKQNYKLTVDSLAIDGKWILYGLITGGLIENFNLGSLLQKRISLINTTLRSRPDDFKKNLIAEFKQYVLPYIANGDIEIPVDSITKIKWDQNGINEFIRLHTEMDQNKNVGKQIVVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRL5</accession>
    <name>A0A8S1JRL5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRL5"/>
    <dbReference type="OMA" id="KWLKCES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="65"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="303" mass="36190" checksum="533C7BF3CA95A6E5" modified="2022-10-12" version="1">MENLLDSQHIEAMINANIKNIIKDNSLNKEIFNLLSDQQQQISQTILVDDPVYINGHYYYPIKTGNIIVNRRYGEFVLLAEYLFKTQPEILQKPLPQKEFGVIYLFSFIQENTQLLKLRKQKFQIYINDIKKQLPNNQIIIKFLENPQSFYEQIVNIDYSLIQQQRYIEKIKNFYETKWLQSNNESGELNTKKELLQTKIIHLDQIQTHLVILYLNLQYKTKSSEIYIQQNEIQELVNKIIKNQELYQDDLSANKLERLTKKIGEVIELYQNVRTNKNQFDSFEKLLDDCLKLVIFTLKELQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTI3</accession>
    <name>A0A8S1JTI3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090342</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTI3"/>
    <dbReference type="OMA" id="SQRQKKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="469" mass="56430" checksum="162334847A020375" modified="2022-10-12" version="1">MLNSNVQEIKQSSYNDYIFDSWAKTIETSRVQTHQQTQLKIQSQRQKKFKYSNILYQNNDQIITTKSQVHQKYQDVQLNSFSNNLDELKQKEQYSFQKYQKVRSTNRNHSICRNQNEDLEIKYESIVNYSTIFSQRIKNSQKQSLKYSQNCKLILPKIKQKQTQKILINKFLVICRVIGKIIILYFKTLSNTNPLKITNYLKLQKVLNLRKIFKYDRNINDSLSKSIREWVQPSLQKIFYHLQNTLIYTQDEKVLDLRKLKDQEQIWTLNFAKFLFQNLELITRKGNIPKEIIKAMSNALYQENNQYVQLFLAQRTKFYNYPFSILEMQLICSEYILFNAIVIELFDQANNLKYQSFNHNLNCKIQVLKLTSIINVFYINIFKDMPIINFDYKEEQIYSRKLYITNDQEQLLQLVDTKEINNSETLILGLKHQEYIQRILQQREKWKQKLELIFNKFINNLSSQIDISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXY3</accession>
    <name>A0A8S1JXY3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXY3"/>
    <dbReference type="OMA" id="EVVKWEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="472" mass="56749" checksum="6C7FA4123D935DE3" modified="2022-10-12" version="1">MLSSNEVVKWEWQPFNNNIVHNGQTLRIEASKVIKYQNSNQGYSFDLKLWKPFPFSANIGWNWHKSWNIPELECQLKIDNDFDHHILNTLTIVANLYIVKHNYDKMEMESIGCKGCPEGVFRNGISILRQLKFNTTSYKNEYQKFQIVVVISQINSSQQYVIDAKCSPYIFVDSRKHARQQALNNNIQLIIDPFLPENLDKEFVKKERKNQPKGLLKVDNTIQGLINYFTAPNIRHKIKHPLFLAIKFDKCVKLYLQRQNLVKNSKDIILKVQSILMKSNQERQYSDKGMALLFQIDQTNELEFKKVYEITQIFENDIFMLYTKQDQIPEDYIPIEETSRLKYYYKQLYPNLINQKSGVNYSSENQEEQPIIKQVTLNEKENENQIDTHLQIKQCQQQTQQQEDINNNQLINYKNQLSYYYNYQQQQQQQLLLLQQQQQQFQIQQQQWYNNMQQQLQQQQQYYMMQQYHRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K065</accession>
    <name>A0A8S1K065_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K065"/>
    <dbReference type="OMA" id="PYLNHIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="585" mass="69819" checksum="69BA0E8F7DFD3459" modified="2022-10-12" version="1">MRRTTQTQQPTLFDQIISQTNKDEQSQEKQSKQSKVNQSQQSTYNKKSLHNLQEIVYQKAFDYQEYKQSLRYNNKNLKKIVLQNINNPHEKLIQSLIKYPLSSLEELTLENVEVTDSQFQIFQQKGGLSKCFPNLTHLTLNCPIMTSLSINYLFQGIQTLQHLDLSNTQIDNQGIYNLCQLKWPFLINFIIQWCSRIDNLDELGENAENFPNLQYIDSRFTQTDNWAYNSIINSKISVTLNQLRLFGTHVDLETTQWKSSYLMNLFMKTMKDYNFQEFKIQNNLACTQSSSDYKQMKINTLISNESQKMHLNISWKITPQQGVLQFVNTAENVRNKFANFGKLFEFLQKQWQLRRSLLSLCIIFDQFLCDPNNFDIVLKTIIDFKELKKLKFYVSNQTLLNPSNYFFYFNQIGELKQLMYLQLDLQNNEIVINESFLNRIIRTIQQLSYLNLFYLNIRFNEIKNIKDLHNFNSQIINSIKNPYLNHIYLIETKEENLNYRFKQALSLIHSNASKFININQFQNIILQEDAQNYKEKIFKYQTELFYKKKMQLIRCCVLSNFHQFNYGNRFRQQVIYQILDMIINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0I2</accession>
    <name>A0A8S1K0I2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0130037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0I2"/>
    <dbReference type="OMA" id="SAWIVKK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00177">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000044">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF56">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="START" evidence="1">
        <location>
            <begin position="25"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="209" mass="24026" checksum="218C68667D853577" modified="2022-10-12" version="1">MQNNYDQLLEETKTKILSIVELKPENQWTLDTNKDGYIIYTRNNPENGLKINRTETNAAVDPEEFINLVIDMTRKKEYDSNFLEGRLIEKLDQNTFIFYARGKPPTFFIDARDFCLLSRVYKLGDDHFMTITKSIEHPQVPAVKGVQRGEIVFSAWIVKKQPNGQTNIIIIGNMNPKGDIPKAIINQGAKFQAEGVKKAVEYLTKNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2G1</accession>
    <name>A0A8S1K2G1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2G1"/>
    <dbReference type="OMA" id="KDTHPWG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007304">
        <property type="entry name" value="TAP46-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933:SF9">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04177">
        <property type="entry name" value="TAP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="333" mass="40040" checksum="60F5E9EDE87CF7BD" modified="2022-10-12" version="1">MNSEEEILKQFDTLLKQVNIFEEQTPGDDKLMELIEKFQQTQQAIIKQHIFSPNEEFNEIKTEHLKFFLLPYYVGWALTQLQNEDIRQKNIETSNYYVKEFLKLLRQYEILKGIQLKLFKSLMENDTLQINRDDKIANYKEQQLLNKQIQNLQKLDDDKLSREIITLQLNQKIISSFDILNTNQQELEILKFKSQMKKDKQLQQQYQDQQEKPIPKMKVWNIPKPTMQPQFFDPHCQHCQTESELRQKHVTEVWQPNAARLPNMTLEEFADSEMKFAKDQEAKMKKAQEEQQKLEQDKDDDKDYWADQQTLKDRNWDDWKDDNEKGAGNKMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4I5</accession>
    <name>A0A8S1K4I5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0130050</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4I5"/>
    <dbReference type="OMA" id="TLWRGAI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000009">
        <property type="entry name" value="Mitochondrial 2-oxoglutarate/malate carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="15"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="116"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="216"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8048723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="35102" checksum="A8C6096CE7A326C1" modified="2022-10-12" version="1">MGENVLEKHWLTKITDTLQPFGIGGLSGCVATCFIQPVDLVKVRIQLKSEKLGPNAGSEISPFRVFSEILKEGGVLSFWKGIDSALARQVFYTTTRMGIYKTMYQRSKQANNGKEPSFLAKSWCSIVAGFIGSLAGNPADLALVRIQADSTLPVEERRGYKNVFDAFYKIVKDEGIVALWRGSTPTVIRAVVINVAMLGPYDEIKERLNHYFGTKDTQQTRLLASAAAGFLSSFCALPFDNAKTKMQKMKKDAAGVYPYSSIFDAMGKTAKREGIVGLWVGFPTFYFRIAPHTMITLLTQDWLTDKVNSWRKKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7T8</accession>
    <name>A0A8S1K7T8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7T8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="242"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="417" mass="49483" checksum="7A3DF2E236D8A9AD" modified="2022-10-12" version="1">MLYDSKSSKIFSPPKIFFDLDKKRNDAMDIVQHQHGEVQDYIEQVNDKTLIAIQMEEQRLNQRYFHLLNQLEKTKQQIIKLEEKLRQQNDLDNHPQIKQIISELSKVDQYRLALIKKTTQLTQELQLNQFTIKELKEQVQNKRLQIRDCVRDCWQIQQQIINSKSNYTQSTSRTQRIHLQSLDISSNRSNINLFITNQDTNTHQLSLSQLPPIKLKKPSKIILLEVLQKASFRGQQQPVNDIIEIYKQINILRRKITEQKQEIKKKQNQYSSIRNLLIYCANLSLKQFKNKQKITNHNGYANSIYFDFNTSINQSITEISSNPNRERKIQNILYDTLQQIMINMIDTKQKDEQNSTQESIIKNAISIEQFMQFSSEQILGLLALQPRLMHELLEKFEIKYRQLGMLSQKMKILTQNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCR7</accession>
    <name>A0A8S1KCR7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCR7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="5"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="85" mass="10096" checksum="6343C7ED8304E4C9" modified="2022-10-12" version="1">MNSLQIIEFSNRVFDKYDKDKSGFIEIDELTFLLNNLAKEIKSQPPTQNEINYIVRYLDKNNDKKISRIEFQKLGQLMVKVLEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDF0</accession>
    <name>A0A8S1KDF0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0190302</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDF0"/>
    <dbReference type="OMA" id="CDIQNIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001236">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="76"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8052677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="362" mass="41975" checksum="7FDA46125D77C848" modified="2022-10-12" version="1">MICSLDLCNTSISLDSLMKGLIIQCLWCPNAFYCCEDCRQSDKQLKAINSLSKHKSQCRGIQQTVLVDKQNILKEMQLLEQIGQGSFGTVHKILIQGQLYALKKMNKHQCSKEVRIHKHLNHKNIIQFHYFIEDDEYVYIIMEYASQGTLTMNRDNCDIQNIFLQLCEATMYLHQKGIIHRDLKPDNIVLDNFWNPKICDFGLATYESVISSFSGTFEFMAPEVIKNLPQTQKIDVWSLAAILYWLLEGKPLVQGNQIEKMQQILDFKKPQFTQITNPYIKDLLNQMLDPDPDQRISLQGVLNHKWTQSINTIEKEINDISTQPSVQRLQQIEIQQQLQKHDSSMLQKIISLFGCGGREKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDT5</accession>
    <name>A0A8S1KDT5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phospholipase D</fullName>
            <ecNumber evidence="2">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0180386</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDT5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000011">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="7">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8051895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="397" mass="46119" checksum="66218727AF975C28" modified="2022-10-12" version="1">MQEKQLRQTIEDDDEEQHYYILENQDKQKSKIQDEKFDQIDEQDYEGMQAKMNLEFFIPHIDMNQNQQKCVTQLTRSSGTWSTGIKQTEKSIQNAYVSLIKNAQHFIYIENQFFISNTAGNPVKNLVANALISRIKEAHEKQQRFKVIVFVPLLPGFEGEIDQSNSAVLKVQLHFEYQTISRGGKSIIETLKQDGIKPENYIQFFGLRQHELSPEPNSIPVTEIIYIHSKLIIVDDEIALIGSANINDRSLLGNRDSELAIIVQDQIKVDSIMNGIPYKASKFAHTLRTALYMEHFNMKYEEVIDPLSLQFEKESTAQANINTRIYKQIFACYPHDDIKKVSDYEEFKANKHLDEYDKLKQFIKGHAVTFPLYFLCEENLNIKVTQKEYLAPEISFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHA8</accession>
    <name>A0A8S1KHA8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140393</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHA8"/>
    <dbReference type="OMA" id="WHIPISK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="162" mass="18939" checksum="E8DCD1C00C58F658" modified="2022-10-12" version="1">MGAACQYQQQGSDTCYNQETQWYIPISKEFKPKAINTSIKHDLRANYLELDCQYCDKPEMKEMGAQTQVTFYNINQCVTQKKKSKTQKKQKQQKCREKVSFENSESSKKRSVSQSSKTQNKHFLYQSYTNQRKNQVQDFQPYVVLINSQKVSPSHVVQVSID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIT8</accession>
    <name>A0A8S1KIT8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0230149</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KIT8"/>
    <dbReference type="OMA" id="RQKRIMK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="3">
        <location>
            <begin position="194"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="19"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8055380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="288" mass="33758" checksum="F0A9B860476D6732" modified="2022-10-12" version="1">MTDLEENLFQKSEVIKTQFQALQEKVQNFVEEIKILNDQQQSLQIATLQYNDIQISRKQFKIKAEKTKELIAQMEKIARGNEQKNVANKIKQQYLKEVDRQKRIMKSFVNSSFEQKFSKISVIRQSKAHGQETQQEQLKYFVDQNYVKLVSKDQRVSNNERSNLSQVHAEEINRIKTVSIDEDYIENDIIDLENMLIDERQRELDLIEKEAFQLNQIVNQMDEMTDQQGQELDIGQQNLNQVKTNIIGVSNELVEADQSQKQSMKKYKYVIGGLIVVIIVLIIVFIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KK87</accession>
    <name>A0A8S1KK87_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0220214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KK87"/>
    <dbReference type="OMA" id="QIRHENY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="429"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="822"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="579"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="149"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="429"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="509"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="523"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="823"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8054801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="940" mass="111776" checksum="E6C78C36B37A6DB5" modified="2022-10-12" version="1">MTEAKSKRDLIWEEKRRQREERLLRMEGQMLENNANQFQLPPPRLDDQVPISFPSQPQEYEPYIPPEPYVPIENYRNPDPPRMYQGSIPQQSDFREQRDKNSQFQQQPAIQQSPLKNPIKQQQDEYREYLKKQMEDKEAEKKRRREGTNNNQQQIQQTQFQQQSANPPPPLPTQPINRQKQEYGEFLRQQMMEKEEQKKRRIQSGNNQNPQSQYQVQQQSQQYQQQIYQPQQFEINQGSYSQVNQQSIIPYQQQQPQQFNQNKQQKQEYGDYLRMQMQLKEQEKNDRKKNLQQRMETGDAFPFGKGGGGAPNQRGANIQVAQNYNQQQIYQHQILNQQQPQYVQQQQSFQQPIQQVQYQQSIMTPQQYQYQEQNIPFDQQPILTEPQGDPQLKGGRNRLMMENQNIDDIKKKEQQKIEMQRVLQEQIEEKKRKKEQERLQKEMEDRQEEERFRRQKEQEEMEKKREDDLKKQQQGKFEQDNIDLKEKRRLERIQERKNRKNQQQDEDQPQQNFQGFEQQQQQIPPPSRNLPPSQPKSENYLKEFLMNEKMKEGVSPHQSILDFYRNGPQPMNKVEIQQNREIDDIKRQMLQQQENLQQQIQQVMSQAQMAIEGRNKAEQELIALKEQVKNKYLQEERHHDDLMIALVKNDPKWEKQVFPPHLLAQAEVGAYKDLSMPALNENFNFPNLSQNKAPTFAPAYESKPLVLKEESKEQKQNFREQFGLQSLVSDSNMVPIDHNASLVPQIQPMNKKVSEVQLDKIDELNAHFEDSLKLGIGETYQIRHENYKQRPPSNNGQHPSWNVPSIDSEFVKEIDDLLKYKPAQQTLNRPPSGFTRNGLEQKQLQQSISNRPPSQTNQRPPSTFGRVAQGILGSTDLNYKGITLDSEFPDMTMIKKVTNTDTMDIAGIHKKMDQKLMHLNEIEEDDDLKKLDKLLYQYNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KM81</accession>
    <name>A0A8S1KM81_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KM81"/>
    <dbReference type="OMA" id="IMYKFYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002276">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="88"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="325" mass="38358" checksum="F523434B7A12995A" modified="2022-10-12" version="1">MEFNCIIERESFQLNVKDRSLQISNKSNQYSFDLSLKILITWLFRENEINGFLLENLVVEGDGLNMKKLKQFLSGKITFSGIQIFYFFHGEINVNINKATKVCTLINRQTKLKFSCKCFKKSVLGIEKIHKEIEIIQKASKHGFAPKIYECFESNNCIYLIMENLERIQDQEFMDDDIRVFLYNLIQTITSLHKENIVHKSIKMSHILFSEDNKLKLVGFGQANDTNIQNNEFRLDIFKIGMIMYKFYQKQNFQIDQLPVIPDEGNQLIQSLLDWRNDSPFQLNLLLNHSYFKLLKNEGIKFQIQCMSPKFKTQVYQNEDDESNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQ52</accession>
    <name>A0A8S1KQ52_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQ52"/>
    <dbReference type="OMA" id="NQSRCHT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="392" mass="45700" checksum="B9CB2528CBF2D8CF" modified="2022-10-12" version="1">MDDIYSQMTDKFNCDSSNRTDDYFMQVCQPLSSNRCQQIEEIEFKFRQSKQDYVFPRIPFEETPDKYQTIQSPHRLKTEGNEKSEDQLDLNFNFNSSSEHISDDAVVLQPKTYQQKLKPPQPKKPKNAVTQQTYRTILNDLERKLHTINCKDFIKKKYEQKGASRAKSNSNSTTHYNSHYQRKPFEIDTKTSSVKKSIPTESKKSLSKTTLAAILLKQKSKSNPSKTNNIFNVNNSHINLNVNINSQTMDLQQQALTVAQILQKKQLLNRSSLSSYQRNSNRCSTEPLEEMKARLSNNHQKNKTTVTNQSRCNTGMDVRAQSTIQYLNQMKKQQIKKYQKQQQQQQQIQQKQQPTMQIKPQQNIKQRSSTNTSKPRISSGKYKKYNLSIQMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQD2</accession>
    <name>A0A8S1KQD2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0260165</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQD2"/>
    <dbReference type="OMA" id="ARSICGT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000048">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="43"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="8"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8057680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="292" mass="34364" checksum="AF53577CB3FE4B34" modified="2022-10-12" version="1">MGICFGILDEDNKEDKKKNPNPIEQQDSQKEKEEEISYQLENFTFKNVLGTGAFGKVLLAEHIKTKQYFAIKIIDKKQLENYNFVESEHQILQQTQSPFIIKLYFTFENNNKLYLGTEFVNGGDLFVHLKKNVQFSEERTRFYAVELILALKYLHENRIIYRDLKPENILLDQFGHIKLTDFGLSKFKFSQITYTACGTPEYVAPEILLEQGYNECVDWYSLGILIYQMLCGTIPFYSQQQQEMINKRLSQPFEFPKYLSQKAVDLIKRLTENEVHNREFHQGFKTLRVQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRU2</accession>
    <name>A0A8S1KRU2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0260315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRU2"/>
    <dbReference type="OMA" id="QTWINYC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="223"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8057978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="334" mass="39599" checksum="B21655DE8FF82BD2" modified="2022-10-12" version="1">MKRQGPLFRKVESPQLSPKTQSQLQQQQQQLQLQSKTQQNNAEQQIQNKVQNYLSRKSVNLQRLSQRMDDINISKRSILYTSSAERDLKISSRFFKDFIGVSQQLKNKEKQDPELLKESYKYWAQAIKTKKDPQKKKKVGAVFSTSVRMKLEKMRALILDCVKKLRFMKLDPELLMQNRTILKNPYQREGSYVFFKGVTKNDLDLVKLMLDKCRFYAFDVNENFQTALHICSRKGHLQIAKMLFQHGTYPDVRDVSNRTPLYYALINNQKELVQLLLSHKCNPWSFSGCTYETDDPILTKMLKIARRLDMLLMMTPYNTRQQTWINYCRVFYEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KY36</accession>
    <name>A0A8S1KY36_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0270188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KY36"/>
    <dbReference type="OMA" id="YHINEML"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016286">
        <property type="term" value="F:small conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015449">
        <property type="entry name" value="K_chnl_Ca-activ_SK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10153">
        <property type="entry name" value="SMALL CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="2">
        <location>
            <begin position="239"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8058383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="423" mass="50108" checksum="C935E30DD213DA56" modified="2022-10-12" version="1">MKIIGPKGLEDGRVTNLYFLKFEFMEQVCFVFNLVGIFLGAIQYELEFEEYDSDDPFRDISQWLLWMIAGSTILLIYFTFYRYQTKIQWLKSRGVIGPREKFYDINENFYMIIEIIVYCLIPLSFTETMRISFYNVAQGAEAYYHINEMLTLLMVIRVVYLYRTVLTLTFWSSNRTLRVCDFYSCEHNYLFVIKSLLKTSPYLTQFLMLLSLIFVFGYQLRICERPLTRVNDIIDNLGVYYNSVWCIIITITFVGYGDYYPRTDLGRFVVIIVCIVGIFLLSVMIVTFIESLKATPIESQTMSLLERIRLRQIVKNEAAQVIKLSMQQAYRWRNGTLTEKKQHQIEIDLRLAMHNFRFALQQQSKFGMDNQTENIINHIGLDLKQLRNEQNLDVSLLMEKLEQQEKISKQLMEKMNLILSEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1S8</accession>
    <name>A0A8S1L1S8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein Sm D2</fullName>
            <shortName evidence="9">Sm-D2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">snRNP core protein D2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0310054</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the snRNP core protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8060931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1S8"/>
    <dbReference type="OMA" id="IDCRHNR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030532">
        <property type="term" value="C:small nuclear ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01720">
        <property type="entry name" value="Sm_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000156">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027248">
        <property type="entry name" value="Sm_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12777">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="19"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8060931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="103" mass="11962" checksum="9C57A78F503CEC52" modified="2022-10-12" version="1">MAETQKRTAQEETLEKGPFGLLTITVSHNTQVLIELRNNRKLLGRVKAFDRHMNMILENVTEMWTEIPKGNKGKKAHATNKERFIPKMFLRGDSVIYILRNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBP4</accession>
    <name>A0A8S1LBP4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0360132</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LBP4"/>
    <dbReference type="OMA" id="DKCSKWL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="221"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8064449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="271" mass="31879" checksum="6418794B90DFC874" modified="2022-10-12" version="1">MIIDQNPKQQQKTEQKQQELNQTQKLGIIVKNLIIIKKINISHPQFSIQLIKMEANQVNDQVIKQLLYSKKYKELVDAGTQLGLVKSYSKMKYDGIIYSIGQIIQLSVDGNRDKKTYGKLIGFCNLQVDEYLIPMIKVQKYINKLELPQKLKELQEGISEFELFQSEIEEWLFCTQIDHEIKLISIKEYEEMTIQNERTYFTRADYNVEKDKFTPPISKWTRICICNQISNPDKSYIQCDKCSKWLHYECAGVQAQLAQDMTFYCAMCRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LEK1</accession>
    <name>A0A8S1LEK1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0390129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LEK1"/>
    <dbReference type="OMA" id="CIRINPY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8066518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="262" mass="30924" checksum="9B66417E312FE8C5" modified="2022-10-12" version="1">MSRQCHSVQKNSKFSGKQTNLIQGQLNNDFIRKFPQCAFISTPLSGKSLSAICSPETQKLNEKMQEELSQLDDLPIYHFPQMFNREEDKMQYQVQTIQFFEKSQMLEAILNYKRQCWDVILPINEFISEQNSQGFWILKIITKKYIELNNYDNSKELMNFCLKLLNRNLHINHDDIVQQLDGCIRINPYCLLKKSSIKTSISNLLSNLLFENKITNNLEIFKTLFNDFENKDLDSLKNHNNLDIQLKYLKEILINKLLRISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFK2</accession>
    <name>A0A8S1LFK2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0320069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFK2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="92" mass="10800" checksum="0E5DA733EA496927" modified="2022-10-12" version="1">MQIIAGQYYEDSCLFYNIENLNNAMFSYMQQNHSSNDLYDSAQQEIENPEAGNNPINPNNFQFSSTDDQKQDESYIKIQKQNYQYKICGSQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LN27</accession>
    <name>A0A8S1LN27_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0420051</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LN27"/>
    <dbReference type="OMA" id="KDSHEYA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000019">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF46">
        <property type="entry name" value="THREONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="250"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8068229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="609" mass="71934" checksum="D27E4240F2CE512C" modified="2022-10-12" version="1">MKIIYKFTQQVLYQNKQYTILKPSQLSVPKEAFIAKVINKDGSTSLHDLNREIKQDCTIEFLDFSNQDARRVFWHSSAHVLGAILSKFGAELEFGPSTENGFYYQCKLDKEIELDQLNQEIEQYLKQKKHLFEIQNISYDEALKLFNKNSYKLSKIKQTALQNYQIYKLDDFIDLCKGPHIDHTNRIYKLIINHSQPYQNSFRYQGIAFPNSKSYLEYQKQQECDHRHIGKHQHLFVFNKLAPGSAFFLPHGTKIYNKLIEMIRKEYRERGYQEVITPQLFHKDLWKQSGHLDKYAENIYMTHDNQMGLKPMNCPGHCLLYKTLQHSYKELPIRFADFGVLHRNEVHGTLTGLTRVRKFTQDDAHIFCTIEQIQQEIKNTLDFLSKVYGRFGFTWELTLSTRPDKYIGDLQIWDEAERQLRIALSDTPYTENPGDGAFYGPKIDVLIRDHNQKQHQCGTIQLDFNLPERFDLTYHHENDKFSRPVMIHRAILGSIERMMAILTEQYNGKWPFWLSPRQVMIIPVSEISKSYAKEIYDQLYKLGFEVELEDTEQTLGKRIRKGEMLHFNYILTIGVKEQESNTVDVRERGDNHKHQSMKLNEFINKINLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LNX1</accession>
    <name>A0A8S1LNX1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0400098</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LNX1"/>
    <dbReference type="OMA" id="QRDAQYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000243">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="20"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="75"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8067073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="235" mass="26935" checksum="58FDF7138429BA76" modified="2022-10-12" version="1">MKGLNLLKYTLPGEQPINLILSEKERGKLFLGNLDCLINQQILETNNINCILSICTEEKIIVGPKYQQIYLDIHDNMNSQISKVFERSYLFIENALKSQQNVLIHCAAGISRSATLVLAYLMKTYQYTLEQALRFLKQKRPYVRPNPGFLLQLLDYENMLHGCITSILGPELTPFDIQRDPQYKPQPKVELRKSLPLKQVSGPSPVARVERKTHTEKLTRVSQSISTFQPKAFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LS10</accession>
    <name>A0A8S1LS10_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0420286</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LS10"/>
    <dbReference type="OMA" id="FCKNDCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="7">
        <location>
            <begin position="52"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8068691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="140" mass="15766" checksum="4A8BC4942E28C9D1" modified="2022-10-12" version="1">MLKNSQISCYSLQKSGESQTTQDTYNNTYKGQKKSVLLILNQSQLIDQPLEVQSMFIFPSSADESRTPIFIDCPACRQKSETLIAYQNGNYTFICALLLLLLCFPIAFLPFCKNDCKDVIQHCSKCAATVGQAQFKPFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LVW9</accession>
    <name>A0A8S1LVW9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0450233</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LVW9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="136" mass="16766" checksum="775290D38B30DC80" modified="2022-10-12" version="1">MMQYFTLEYQDKYCNQYEKNKFKNQQNLQFYINKQKIRSKNSIKFFKNLVRSSYQVNRFSNNQYKKESEQIPHKNSESLTITNNEISYIKYLEQLSNGISKKYVIEYFKKQKIKSDQFINLIYPLLQIEQELFEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LY58</accession>
    <name>A0A8S1LY58_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460198</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LY58"/>
    <dbReference type="OMA" id="KIQHFDA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="129" mass="15404" checksum="4EB5AF81D48AC585" modified="2022-10-12" version="1">MLNKALTYCLQDDDPIRISELIGIREYQKQPKLCQSTFVTSSDLKQMILNQKRENSVQSYRKIQHYDGDLKPINKTFDCRLPLNLNSCRKYMNLKLVQEPTKPKLYFGIKHQQFKIKIKMQSKRLEDMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYD6</accession>
    <name>A0A8S1LYD6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0460021</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYD6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8070553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="24451" checksum="581126DCF34F8C05" modified="2022-10-12" version="1">MQQQKREKTITFQGGQQQIQQENQYQPVKGQHSEGQQYQNNPQSQQIIPFSAYAQYAQYPQQLNNASLYKTDQPLHPIDQKKMDLRSFKRLYRFLCKIVFLLSIFYCYVNYLPLHLLDAMLNFNMEEEQRGQLVSCLLSRLSFLLLELVQVYVQKGPFYITKLQWSQVQYLKQVDLLQYLPLLIIQTIIYIIKRMKKNNQKRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZB5</accession>
    <name>A0A8S1LZB5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZB5"/>
    <dbReference type="OMA" id="CFNICTT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="91" mass="10820" checksum="67283EE9249FFE94" modified="2022-10-12" version="1">MLSEDNRHLFDQYRQTLKSHVFGPEISLNQVVAKQLEDDLKKKQSVSFKEEDDIIIVENWKEYNVDVSSQNFHQKQKQKSHSQCFNICTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0M2</accession>
    <name>A0A8S1M0M2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0450278</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0M2"/>
    <dbReference type="OMA" id="IIHIANQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052969">
        <property type="entry name" value="Thr-specific_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763">
        <property type="entry name" value="ALPHA-PROTEIN KINASE VWKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763:SF1">
        <property type="entry name" value="DUF659 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="3">
        <location>
            <begin position="303"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="270"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="419"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="208"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8070476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="502" mass="58997" checksum="AB4F1AFC98D5BF59" modified="2022-10-12" version="1">MPPRKTQTIKEVQKESQSIIDQKLGSTRSKKVKKNESEEDSDFITASSKSKQKNSNASSKIKSSTNKQKESINESKDDPQDKLSPELKPSSQRQKARTKGEKIQDKHNTKTEEVKLKQQNEDENKNVDNNHQQQGNIQEENKDNNDNNKDNNNDCNKDNNNEIKQQQININNQNQNDDNQENLKNNDQKHSNLLQEMNENNNKEQLDKQNNQEQKQIQIDNNTLQNQRNNIDESNNKESDEQKDEKHSQQQNESVVKSIPQIIVNEINNKTDQQNIRQNEEEEKEQLDKQIKVVQQNQETDVDIVFCCDINLLEGRSVDQLKNTIEFIIQQLKEKEHLSSSIKFSIVRFNDLITNQKLQQCQVIDLCDETEILQSLEEIKQMGQIEIKKGLYYGLKLATQNTKWRQQSNNKSYRYIIHIANQQLELDNQSDELEHIIQEMNNKNIRYKYLRIDKRIDLDFDGKQYLQNKLNSYEESFLKEFGCLKNVVSSIILRELIHENNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M6R2</accession>
    <name>A0A8S1M6R2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0510087</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M6R2"/>
    <dbReference type="OMA" id="YCCASIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="312"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="897"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="433"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="314"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8073501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1165" mass="136495" checksum="9AB2A4DC5AD1929A" modified="2022-10-12" version="1">MSVIFDQLDNIQLLRFRFTQVIKLGVSIYYIENCNLKRLVVTDNQIALYNESETINVLQNVSQICSDGKTLFCLQTNGDLYQIDQQPKLLISSKNYMSITPTTAIDHSGRGFFWKDNKPIYSHCKHISTYQDKMTLITIGGKIFNIENNTQIPIQQLNGLEINGYFKKSLLFQFGGIAITENGDAYCYNTKMIKLKTSNLEDIQASNRFIFGIQGKYILQWNLDDFQNHQFFVNQTIFKKMNYGHEIQINCKKKEFQEVKFIFSNQYSQYCCASIQQISNKNQNDQSISILERTFCRSTFQNLKQQLFDQWDPPSKRKAQEVRQSKFLRGERTDRSDASNRQTDRIDRKDDISQQVQKHQQQNNNILIEKQNKQDKQINLQEQKCLQIDSNFNFKNSLKQSSDILAPLEISKFNLNDKIQKQETQQLNNSNKSDKLVDQIQQKQYNLKEQKQEEQLIEQGLNNQNNELQQKLQNEQKIEQQQLLQQQQLLQQQQTNPSEQQQLNSINMPKQCFQEIKQPSKLLEQQKKQFDQILISCNQQNVQQQTDNFEDFQKIKEIHQENIYSQQNIIRQKLQSIKEIYDQTRFKTQNNSLYTLDEESSVEQSICQQELEKGRFFQRQTLQINDLFDNDFKQVSNNQENQKLQFSSKDIHSPTQSLENKIKFPQVNLSELQLPPIQSSLNNFLSKLQLKPEKTIDQILKSQTQKEEENENIPQSNPSELISHHQITQTKKIRSFLKPEQMQNQQQKNAVEIMDQQSIIDYQNNSDFIDQQHDSFMQILQTDKKQSENSKIQNIMSIFDQIHIQPIKRSPSKMNMFKKQDQENKMISDSNGQLDYNLLEIKNILIENHPKILDKKLSPPKTNSKLHKNLVTFSQDISQEDIEDRVMQVVNIDQNLQSASHKHSPIKQKSPIKDNNISSSKKYSLQLSEQKVLTKINQNADVRQPSFDVKIIPNISEQKQSLSRLRIQPLNCNAVRPEKIYTPLKSRRNSTARGSSNETNRFKQLQIDSQVVVPEIQQNQTPLINKFEPLDLSNAMYHIDQTLFENTTKRTPVAQVLDMINVNQKSNQKILKEPQLIKVSSSSQIPPSLRTNSVGPISKKTEVSPLKVQRQIKNQPHSKSVNDDTIRLKRRQQDIILRKLFFKLDIHLKLNKLEFMFNLKQKNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MA01</accession>
    <name>A0A8S1MA01_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0510023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MA01"/>
    <dbReference type="OMA" id="QYKFIIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050167">
        <property type="entry name" value="Ser_Thr_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257:SF964">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_50, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="771" mass="90463" checksum="D8C79DFD4D9416D0" modified="2022-10-12" version="1">MIKSNGLVKIIDFGLFGIKKYQSLLHFYTNKSGFTAPELIQQKGLVVSGSQEGDIYSVGMIMYEMFEKKIPFEGLQLQNIQECLNKAVRPKIESKSLMDDIIRWCWQQEIEKRPLVDYGTIVVGSNIYETEFLDEIYIYYGNKTTNTISFKDGLIISPKKLELPEYKINRSERGDIIMAIIKEAMNLLEYSKLSDLGIINMFELQYVNQLKSYPSLRFQLMDREQPSILMDVRKSYFTMHNCYQIIKSLNNIDPSTYFKNKYVLLRYPEKKMIVQIKSVVFDQEFTYKGLCLEDYFKQKYQLRSKSNVYLETYNTQNIKKQFLIAEFCELKIDYIEQDVTIYCQQLEYWNKIINNSTSFIQFLNQYKFIIRNEQVEFKQQYLEPGNLVLNNSTKSIYQIVASRTSSEFEYYYSDFFLKNPTTFTTSMTINKLMILVTREIIELRDNFKQFLEKFDVFIKSRQYKISQPQIYDINNNLNEIIEKYVTEDAYVLFVGDSNTDFVQARITLLQKAIPNQIIILPIQDQEINRLLAMMTSNLGSVPWSIKEINGLINNKRSAVLGIWKTDNSFSACLSINKYLNKYISSFGDLDQILTLLLATYYATHKILANQIIVFAEDDYTQIMEQRIQGVIQLIRDQGLAQQILKPEIIMVQVKDAIAERYFTYFERNFESYYCKNPQVGCIIPINEKQGRFSFHSQRGEQGCLQAQQLNISSANTKEIAQLYYHLIFLNFDLSSTTYLPAPLHYAKKLSKNQLQSEQYKKAIEKGFLLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBT0</accession>
    <name>A0A8S1MBT0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0540272</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0540273</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBT0"/>
    <dbReference type="OMA" id="KQSEQIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="218"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8075573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="310" mass="37274" checksum="E4C31AC3756CAC2D" modified="2022-10-12" version="1">MRKSQSSLLLKSSFMSQRQTLPTLIQKIGVRYATQYELTTQNNIPSHLDNLELIRIQEKYKWKCEELDRTKQSIEREHKTLEDRANQLKKGEKEIRKLTNLIEEKLIQLHQQEESLRERVQSFEQKVKDQEQQFILLQQQLEEQSNQLNIEKNKLQIREKNIKEKEYITELKSKEIEQYIARLKYYTQNTNQKENYYHLQIDNLNSNLQTIVQHELSIKNIEQQLSQQVKSLEKVEQNLIQQLENCKQSEQIIDQQELKIQVSEDLINRKLEWDQKQIISAKNSQTSLFNFENSRMEISHFVHSALSQNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ME95</accession>
    <name>A0A8S1ME95_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0600210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ME95"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8078707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="17599" checksum="188DAC547EB7915E" modified="2022-10-12" version="1">MLSLYVQIEGLIDSMNIINKLFLIIQLVILTFLNQKFRTIFNHKVAQQLKLIIKMCQIMKKLYLIFNHQKIHQLIILGCLTERFDQTINSLHTLVKFNQLRRIYSIVRYFIKGIITSNSLRLNKFMRLIYFHKEMNKGQILKDLNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MET8</accession>
    <name>A0A8S1MET8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0550186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8076005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MET8"/>
    <dbReference type="OMA" id="YLHISAK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40131">
        <property type="entry name" value="C1Q DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40131:SF1">
        <property type="entry name" value="C1Q DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="89"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="62124" checksum="064E5831DC95D608" modified="2022-10-12" version="1">MSQNTLQTYLQNQITSITLNIEHIQKDISLLHNQFEQKIFTTKRELNNRDDSTQIQIEQFKQQFDNTIKVLEQKMLHIDENKMDQNEAYQMMESNRKNIQQDIDLLKNEMAYQKQYFDDQINEKIRHLQISNLTEQVHQIKNQLQQYYQEEQRQLNEHQILTKSLLNANTKAFFQEIERIVQQINSIKIEINDQQQLFIKKPELDTKINQIHLQFELKSDLIEVQNALNTQQIDIAQRFQEFKDEVRTVLELQGNEFYQLINKKANHTEIMSILQNKIDMDILVQHTQDKVPLKDFHQHMSLIENIAVDLGKKQDAITFVEYKDANNLQIAEIQKDLQKKLSKRDFKDILEKKANHEDVSKAFKEIQSILLTKFSYEEFNKFQQNQNTLNEQLCSQNIIGKWLFKGNFLSPGSLIPWNIQAINTLTENFLWDKDKPNIIVVAPGIYEITFGFFAKRKPKIDIMVNGETIINAVNNSSYVVHHSSGKLKDTKNSVTGLTMIDFISLSSRAKINIAYQGDLGEGFLSLRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MF46</accession>
    <name>A0A8S1MF46_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0550241</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1320023</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8076115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8106867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MF46"/>
    <dbReference type="OMA" id="GPRNKMP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000005">
        <property type="entry name" value="50S ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8076115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8106867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24449" checksum="0E9368AF7CFD2899" modified="2022-10-12" version="1">MSKIQSEQLKKFIHEMLTERKKRNFKETTELQIQLRDYDVQKDKRFQGSTRLLHAPYPNIKIGVIGNLTHCDQAKALGLTAIDQEGLKKFNKEKKPIKKWCKPFDILIASESLMKVIPRLVGNVFTKIGKFPIAIPETESVSSKVNEVKSSVKFQLKNTLSLGTAFGTDEMTEDQLRQNLSTTINFLVSLLKKGWQNVGTLHIKTSMGKPIKIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MG50</accession>
    <name>A0A8S1MG50_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0560167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MG50"/>
    <dbReference type="OMA" id="NNCKLCG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="573" mass="67344" checksum="FB64B3F8C71C5A57" modified="2022-10-12" version="1">MQNNCKLCGQSQRMLILEKLQLKYFEAFNFYFAKPINEILSQIANTPHVIYFKDYVILDEQQEYMKRYYKKEEVKPRLDILTDFYVTNYKELHPYLLVVESHTLMTKRNKKIDKLYYQRMQESENPNQSQNHVVVNNVLNSKLSSKSQISYETEEHDIYSSQDQPNKQNRLDFKGISLLNNYEDNKLFLELLDKQYHSINKMTNKYKLLSPNFYIKSAPLSQQNIQTNLIKDLHSIKNVQRRTNNMDLREKKAIFANSQSESKHSQSSFKISKIKDSVDQQVDQFCDYVSGQIEPSDMEGKNKMSRIQSDNITIQKMLKEYQGNQKRQLKSTISQDLLVASTSTLIQGYQGSQKSIKQDQNNNNGSIQNISQRQDQIKKVYQPSSQKKINQNQLDRIAKGGCLTDRNMKQLPISEDLFTKLHDIKIKSSQNAESQKKPEFNYMIPSQYNAMSTFKIANNNKAKSQYTKKTAGFDFATKSQHQQQGNHSQSKVLNINTQKTDDIVKKYIQLATQSIQNHKKFDFKLNLQNLNNHNEPHLDEEFQNKRLNFLTERHSQQIQEKDQFITHRKIVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MI71</accession>
    <name>A0A8S1MI71_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0630136</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MI71"/>
    <dbReference type="OMA" id="MGAGMSW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04151">
        <property type="entry name" value="Arl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000624">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51422">
        <property type="entry name" value="SAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8080187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="179" mass="20514" checksum="D1BFD63D60C6F351" modified="2022-10-12" version="1">MGFVFSKIFNSLIGQKEMRILILGLDNSGKTTILYKLHLNEVIQTAPTMGFNVETLTYKNLKFQVWDLGGQNAIRLYWRSYYPNTNGIIYVIDSFDEGRLKTSKEELMTLLQEEELKNVPLLILANKQDMQGALSETEICEFLKLEEEKSRNWTIVKCSALTGLGLSEGMEWMANAMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKC7</accession>
    <name>A0A8S1MKC7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0630036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8079989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKC7"/>
    <dbReference type="OMA" id="MHILIAR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035774330" evidence="1">
        <location>
            <begin position="20"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="287" mass="34635" checksum="7778CC74E6E63EA4" modified="2022-10-12" version="1">MGMHILIARLDMIVLLTQPESLNEIQQFEIQKYLKLNKSLFTENQINTLIQQIQNKNYEHIRNLYLRRYTRLEELLSLDLSIEQIYDYCIRVYLKEIKKPGTTTLKIYNNIIETHRIQQSYFLREPSIQQLHYNCLDLEQDETNKYLLLSSLYNRNRFEEPRFIYMSDLLRRRMQQMIKDISDRNQTIQKSITQYYHQNKKKSYCLTPKPVRSRNNTFYKNNAKIAGLRVQTEFVIEKRLPARSIYQIKKIQLLDENENVTPNNKNSCKALGRPRSLIRTKFQQLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLT4</accession>
    <name>A0A8S1MLT4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CFA20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0640043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLT4"/>
    <dbReference type="OMA" id="NNINDGH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="60541" checksum="223D408B09320F83" modified="2022-10-12" version="1">MTHLPKINQENTLFDCRELKSQQSENVIFDKTTKSFVLKVGPSNLFTYHNSDDVYIKGSKIYIQIYLIEKLKFILELSNKQEYFRIVVSPNLQQAYSKKLEEVKISSAFILTNKWISLSFDIESFFKDAELQFKMLSIHSLCRLKKIYEGNQPNNINDGHCLIQKICEKRILELLPNSIRAQSEKTDIRKNQLGSQQQRRLSTISNSQEKIVQTESDNKKYSIKQKNLNFQELPKNVHLGLKTKSQTQPLNQNQKKQQVSLHQKQLSNQKNQIQTKSLSQQQPLQSLQIHNQGLNQKGQLSQISSNRSKKLTQIGGNLTKQLSQECLLEKTIKLATGLQVIEEVNINKPPVYAKSSSNKFTAQTQLQKKAVQSFLRHTQEFQNGSHFSHEPFDDSKTDNELGYKYSQVSQGVKDLYYEGDNSEYQTKYGKFSSTFTRPLGLSSYNHRLSSQQSQNQQQGSTNEQFYLSSQQVEQNFSPNQTANEIEEQIDAPEIKSKMTDDSFNSINGLQIKQEDSSEDLAVSKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MM01</accession>
    <name>A0A8S1MM01_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0640106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MM01"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="319" mass="37180" checksum="A981670E826C5766" modified="2022-10-12" version="1">MSWLFNKPQQPVQQPKLPANFAEKVLNLELDVESNAASKEEIQELLELYSQAVEHYSSIKSERYTVFTNKIQALLMKPYVNKMFGAEGKQESKQIQQQHVQKEQIKNNIQFIQQMDSNKQVQQMMTAAIDEKIKRENMIADDFKMQDHRVQQRMLKRIKDPSKYNLAISKSTSLRHLKLDIESQSQQSTLPDTLEQGQDIRSDPVLDKYLEAAAQQQFTDLIKNDYDQVEQLPQQPKSNQENKISIPEPELVSLPIKPSQIKDKLYSYFNDDPQPIDQQNWSLQLGDLSHPKTKKVREMVNKNTELIKQKKELNNILPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNS6</accession>
    <name>A0A8S1MNS6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0670093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="DEECAYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035794254" evidence="1">
        <location>
            <begin position="17"/>
            <end position="3198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3198" mass="353519" checksum="2A7742478D737871" modified="2022-10-12" version="1">MTLFAFLVIEISFGFSVLKMDQCSECSKLIRKQDCEYGNCIWSTNNESIEGICIDNGSNVDQGNSSGQADYCKSILNPAQNCSKVKGCAYFNSSCTFFTGCSAYFFHTTFDCQQISLYCISEGDGCIDAKKCEEYLTQDICENSGSSSGSGHCKWDSGNSKCRDELCSEAGIGLTTDQECSKFKFGCITKGQGCTESPLKECKTYESSGQDCSKLIGLDGPCELLQGSQFCQQKKCETAPITHTKDKQCDQYLKGCVSTGRGCVEKLWPCNTYNKDCTSYVGSDGICENEEKSENCRSRICENGLFNSDEDCNQYKIGCVTNGKSCVTTLQNCSSYKGNKNTCLGFIGLEGMCKGIDDNEQSCSVMDCVEDSDSKYVTDEQCQKIQKLCKTNGMGCVYNLKLCNEYDGSAETCFQMIGSDGKCIGGTDGKCQRRSCQDAPSTYNTDYECKNFQSGCVTNSNGCINQTSCQLTLKEVSCLGTAGCQWNSSCVDSTKCSNFNTQVICENNSAINYSSADGITTYYYTKCSWKNNYCQNLSCSDLNGDKYDSDQACQQVMSNCVYSGYGCVDRQSDCSLFKGSQNRCALYANKCWNDQYATETSPCRSRLCSDNIIFTTDEECAYFQKGCRTNGKGCIEDTKLCSDYQGTQAQCNKFTGFISNSSERQQCYNTIGATITTNCIVKTCDMAVGLTNNTDCTKFLKGCIWNGLSSCVKETSDCTAFYGTQQLCNTFIGNNQKCYGLNTNNAYSQCKIKQCVDNNTPGLTDKQCNDFLEGCVSNGIGCTPKETTCDQFSGTSDTCSKFLGNGQKCTRMSNCTTRLCSDYTNPTKHEDCYNYHSKCRFVNTQTSCIDQTICNNYVAQGNNNTEKTAYCRALKDDSNFICSYQTGDINCSNYSCEQIMTKLECESYTSTKLCYYINNMCVSTKDCSTIIIDNTSANKPTTNSTKLTWCNQYKDINNKQCSYHSTLSSSKCSNFILCEQVVSATSSADCNRILYQDPDTKNKGCLYYSNQCYTLKPLCSDYFAIGNNNTEKSAFCQAMKLNTSVGTDNTKVIQCQYVNGDSNCSSATCESVSVANNQIDCDKQALNCVYFQSKCYTKQNACTSYSTSTVSSNKPIFCSYMKNSSGEHCGYIDGMNNCINSSSDCKLYRWASYNSNPSPSPIPSTNSSKRTYCQQRRSSQGRLCGYIYGDSYCTLETNYCELIQSPSSQLSCDLQAIGCTYIQSLSICATTSRLTTCNDITFDDQILTTDADKLNYCKQVKNYYGYCTWMSGSGYCDDLTSACTSISISSISNDDDKKTYCLSRMSKAGSCAWRKGDTSSACRSFSCFDIINPVNQATCDSVMSGCTYYQKQCITSQANCNTYYADGEDDDKKLYYCSGLSQTTSNLQCTYIKGSSFCTVKTNTCASYTVSALSNADKLTWCYLLKDLSSNICAYSNGDSACKQITCELIISPQSQNDCNSYLSGCQFYKGYCYTKPTNCGSYSIPSTITDNVAFCTNFKNTSNQYCGYVSGSTCAAQSSCTLITAVGVTTTLKQQYCQARKSQDGYVCTYISGTYCSQVGTSLSCTYITGTITNQDSCQSISANCSYLDSTKTCDLNSNLNTCDKYLFSSTIDEDTKLSYCRSVLSGTCNYQFGDTKCSNQVTQCGQFLITNVNDKKSYCAARNSSNGNCAYDEQNESYCSQIKCSDIELPNSQMDCNKRMSGCTYYRNSCVQTQNNCNLYITYGSDDQTKKNYCEGLKSSDNTLCTFIPGVGVCFNQSSSCSSYDVSALTDKIVWCEQLVQSDGQICTNINDASSCSTKTYTCETITANSQSDCDKILENECIYYNTKCYTKQSSCSSYIIPLSITNKQLFCKNMFDSSQKYCTGGSTNCGLPTNDCTNIVANGSDDSQKRIYCRDYKSSDGQMCGYSSGLNCEIEHYYCSVFISATSQEYCDQRIDNCVYIQKSGKCIEKYLVSDCTTMLFNTGLSNANRISYCQSYNPNGNICTWNTATDHCELDTKQCIDFDATGQSDKRTFCQSKQGYNCSWRNGDTSSLCRKFKCQDVDLAQSQSDCDSKIFGCSFYINSCFTLENNCDNYYALGTDNQLKQYFCNGIPTISNQKCTYLSDDYKCRVRDQCTTYNVQNVSDKVNACLGMYNQTGIQCIYVKGNTCNQVDKCETYTGLSNMSNCTTLFDTNKRNCIQGNTSCLTFTCDNISNPTSQSDCDTQLKGLCFFNSTNSKCINISDCSTYKKSDWPLNADPIQHCSTLIDKNGHNCTADDATSILCRERLCSDKLFYLNSDCKEWKSSCKSDGAKCIESTKTCSQFTGNQDSCSKYLDSDNLNFCKVINGSEQDVGPCLSLSCYQNTTANSDSECQSYQNGCLTRGVGCIPNTAKCDEYRGTRDQCAKFTGYVSSTITEICSGDFTNTDQSKCRSRICSDNLIDNSDALCSYFKTGCVTNGQGCIDINASCSSYKGTQQQCSKFMGNNKTVYCWNNIDAPSNYNCIDKLCNHVLGTTDQYCEQEFPKVLINGNLTLLCISNGINCIKNPAKCSDFIGNNISCQTFTALIDGPCKGNLDNSMGYCQPRVCYEAPKSYNTDAQCQDYHPTCLTTGQGCMKFSSYDNQGKPTLNCNSIVSNDVCSLKLGCTLASQCLVSVSSCSQVKSQAICQTTILQNGKNCVYDLSLNQCREFQCNDYVGINDNNICQQFSNNCTTNGNGCEIMKSCVQYYEKLTCQSAYSTDPINRCIWTDQGCRQRECKDLKGTTNQSCKQFISGCITNGQNCVGPNYLCQDLLEKTCLTDYQGNPCIFYKQTCYSYSKCEDMKFTTHEECQQFSELCTSNELNCIPLNKCQYYTKQESCNIGIDGMCGWNNNKCNLFKQCSDVMGTSTPICQQYSNICVSDGVKCVEQNICSFYNSQFLCEHNKGLDGVCIWIDNACRLKQCEDLTTNTDTFNKCFNQLSPMHCSSNGTKCISLSSCQYYNEKSCIIGTDGPCIYKLPFDKKSGQISCRLKQCQDVIGKTLDVCLSAFDSSYKKCVSNGSICVDFNNCSSYTTKIACASGGLDGQCAFTPIASQSNDGICKLFTQCSDANSDKDTCLSNSKYCQWISSGVTKQCIPHTCLTYNSKKDCLPVPSYDQKSFLLCAKINNICQEVQPTTLTMETCYINSAQTYIWNTSTSKCVQCKIKTDNNVAPNSQVSTKSSVLLFTLAILFNLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTD6</accession>
    <name>A0A8S1MTD6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0690054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8082989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTD6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="137" mass="14781" checksum="55646108B503D752" modified="2022-10-12" version="1">MGICHCSKSNQGEQKQVKVKIGGGEDGTCTWKVYRGVTEYNLVTTSGWEMKFELAANGTISGGNKNDNEEGAQKKLYAGTLKNGILDCIATFEDGAQIKYDGQMDGSIMKVTCKVIKGSNKFHLGDIADCVGPVKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTY9</accession>
    <name>A0A8S1MTY9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0630160</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTY9"/>
    <dbReference type="OMA" id="PWITSGC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001435">
        <property type="entry name" value="Probable myosin light chain kinase DDB_G0275057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001500">
        <property type="entry name" value="Probable serine/threonine-protein kinase fhkD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="11"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8080235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="314" mass="35640" checksum="BC717D1FB9FC9132" modified="2022-10-12" version="1">MQGNEDINKVYTLEKTLGEGAFGVVKRAVKKSTGEQFAVKIINKENLSNDDLLALQTEVEILTQIDHPNVVKLYEIYEDDTYFYMVLELMTGGELFERIVEKDHFSEKEAAATLRPIIDALTYCHKMGIVHRDLKPENLLYSSMEPGALLKVSDFGLARFVGSEEVMMTQCGTPGYVAPEIINGKGYTEAIDFWSVGVILYIMLCGFPPFYDEDNDKLFSMIKNGNFNFPSPYWDQISNEAKELIKGLLTIDPAKRLTTEKILKHPWLLNNTHKSIVNLQAKLKDYRASKKIKRIANVLTIARGWGKMAQVKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MU93</accession>
    <name>A0A8S1MU93_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MU93"/>
    <dbReference type="OMA" id="YEHQYHS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="329" mass="39287" checksum="87B621B53D1BD2AD" modified="2022-10-12" version="1">MFNLYSQRGSIIEKDRIKSQGNTLNVPRTDSLLTDQKHKGCLQRINILIQELDRMSEAQKMANLEIQHLQKEIEKLQRSGNQLNQLSTLDEQGKEEELRLEKQKFHQYKQLKEQQIIELENDLIKTHKELKEEKLIRLDLNHRFEIKCQEIERLKQEIDKLQTQSNSINDGGILNQLHGDLIQFKNENKELKKINIKQQSQIDKLLEGQNINQQINQQYCETQQITQQSLEEALFPSNKINPIKKMNRALTIQPIEPYYNKSLYQSNRLSINYPQQQQFKIENNEQEQENDNSYEHQYHSNTKRKEYSNNNCKVFEDTQRTISKQYNQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0J0</accession>
    <name>A0A8S1N0J0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein arginine N-methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0680054</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0J0"/>
    <dbReference type="OMA" id="QWIAKEP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000040">
        <property type="entry name" value="Protein arginine N-methyltransferase-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF53">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="5">
        <location>
            <begin position="132"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8082635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="302" mass="35519" checksum="37BE0569E3F41DC3" modified="2022-10-12" version="1">MDNLNDEEIYNDRNRIEPFVKAITRNKHLFKDKIVLDLNSGMGLLSILASQSGAKQVIAMKANNYASEIIKQNNIQNITLYKEKIREVELNCKIDIIIADWMGNMLIYGGCIQDVIYARDKYLNKDGFIMPDKGQLYIQSIEDSQYKKMKINFWDSVYGVNMKWMKQWIAKEPLLESIKEEQLNSDEQLIYEINLQNCQLEDLSFSNQYQIKIKRQDYATGIIVWMKYSFTYTHLPIHVIMGPTKSPFWKPVILYFREEIPVNKGDKLQGSLAVKFESEELLQIKLSVHMQQYNYVSYFKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N236</accession>
    <name>A0A8S1N236_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoplasmin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0750056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N236"/>
    <dbReference type="OMA" id="QMPLRIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoplasmin-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="147" mass="16678" checksum="E6EF5B7208CDF6DB" modified="2022-10-12" version="1">MFYGIQLSAGESQTIQKVDDHNQFVLSQAVLVKGTNLNVLMNGIVIASLNQKISQMPLRIKITPESQITKLEVKGNGTVHLCGYYIQFDLQNEDADLQSVAQLLKQSNQITKPPVQKAPVVPIKKIDTQPKWDEMDWEDEQFQKRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6K7</accession>
    <name>A0A8S1N6K7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0790044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6K7"/>
    <dbReference type="OMA" id="ILERCET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035847667" evidence="1">
        <location>
            <begin position="20"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="270" mass="29906" checksum="44374B904CCEF348" modified="2022-10-12" version="1">MSKTIILCLIFLSAYQTLSTKNRCDCVLFSNQTECENVPYCSWSNGACGLKNCANIILERCETFFRCSINQDGQCESTGDCSKYTATTQIDCMFKRGNCAAEAIQTNGVYKCRSYTAVTCASQTSENCKNSFENENSFCWLNQQGKCQSYDPNTCSGIPLEICSNLGCDASGTECKAYTCGSNLTVNTCILANDGYFGAKTLCKWNTTTQKCEERSDITELTQNQCSLLTDGGFYWLDNKCTVCPNEDDTDDTSYEILLSSLIIIHLLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N764</accession>
    <name>A0A8S1N764_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0820194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP97 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8089027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N764"/>
    <dbReference type="OMA" id="SAIGCNR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029488">
        <property type="entry name" value="Hmw/CFAP97"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13879">
        <property type="entry name" value="Hmw_CFAP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="183" mass="22085" checksum="CEE7B4C378A793A5" modified="2022-10-12" version="1">MNKSNYYYYTNDSNELLRRDRDFLIHQQKLEEIKRSKSAIGCNRRTKSIKLPKSINIKDEMKYAEIQHNNQLLATNLARIHSRPSQLLQKTQNQQISLKSSQSKEKLNQHTFKILDENINLCKRILDQQSQIDFKQKLQEYKQHKKIKQRLLKVKRQFEQQEKIGFDQRFKSKSFCQVQMNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N765</accession>
    <name>A0A8S1N765_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0810191</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N765"/>
    <dbReference type="OMA" id="EHEGIYC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="517"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8088588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="598" mass="69760" checksum="6E42B0360498BB66" modified="2022-10-12" version="1">MTERHRFSSQQKLPILDLTQTNRFPTEGLKVNITVQSGDAQFVSFRSLNELNPKFGFPINSMDSSQQLNQTIQNDLTQNKKMLLMLGKTLYKQKNEYQEYQENIIKKIRTVTRMQKQVKKVPHRIESFKNNNNDKSPNVIQQFAKKKVTFPMYFNGAFFTKLQHTWVPTIRENTMAADFQKKIYMFGGIGSEVMGDLIEYEIQTSKFREIKQNGDIPLNRYGHCLHSIGYPITLSTQFTYDNQQYTQINGKQLIMYGGEMQFNSALKLRENLSDTRIFDLETETWQLLKPNIENIPESRRSFGSCIVGKGLIVMGGIRSRFSVFNDIHYLNLNLCKWNPLETNKNPFNKGLAFQQLVCAYNKPQLIFEKKDTRSKPYKALEHEGIYCFGGCYIDEIKNTQFYNNFMALLKLDQPNNPWVIPETFGKQPDPRIQHTASYVQEMNIIIIFGGRDDSRSHPYFNDLFAYKICEKEWVQLDIYGNIPRPRASHSAVAYKSQVLYFGGVSMNGYLEFSVNVAEFNQNQIRQLQAEMKAYEQTTEVQTQKEECQRQSILSKQYFSLHQIAPIQKPRNMTKAEEMNQQIQQLSFSNFPLHLKQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9I4</accession>
    <name>A0A8S1N9I4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0840060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9I4"/>
    <dbReference type="OMA" id="STQDNMN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052945">
        <property type="entry name" value="Mitotic_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43628">
        <property type="entry name" value="ACTIVATOR OF C KINASE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43628:SF1">
        <property type="entry name" value="CHITIN SYNTHASE REGULATORY FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="816" mass="93991" checksum="CD9DD2380276B037" modified="2022-10-12" version="1">MDRSTHSNARPASGSRDKNDQGKKEHVIAQLEQIVMDRLKNTIKDIEVEQERQMKQKQDQGKNDQVAVALWQKIKGWATEQPFKRIANQIVDNQKIPQEARQRYSKPFLDQQKLIMNIEQDKSKVKIKLNEYYQTLAKSTQDNMNSISVSLGRLANAGCCGKGEALKNFENEYRKIELDFIQWAALPNLENNNVDIFVVKTSLNYDDFRSFLIKQLNDYFPSAAGETKIIEKPVDKVVYVDKPVDKIVYVDKPVEKIVYVDKPVEKKVIEYVEKVKEVPVYRDQPIQDDRLRTAHEMLYGIGRPQNITEALNLYYELAEVNQDVNSFNIIAQIFSEGKFFPKDLNKAYTYWEKSAAQQNAEGLYRIGRLLLDQVIDGQMLSQGKLQTRDEQKSEAVVFFQQASEQGHLDALVDLGEIFENGLKSEDGESYIQEPSLESAEFYYSQGKKVRYPRAINALGLFYYNHPDFQEKVLGNNYRKALKYFEIAKELGYANSLYWLAQCYEFGYGVGINLEQAKSYYKEGALKGDVSCRLQYMHYVMKDCSNSGRQEDYLFAHQYLIQIMIQNPEITEVYFYLGHLYECGFGVQKDPQNAIHYYFRGAKLKNPTCMTKLGDCYHSGFGVPQNQREALKFYKEASELKDSEALINMGLIYEQGYEGVSIDYAKAFNAYEESSKLGNAKADFHLGLMYEAGKYVKKDVNYAIQRYQKAAQMGCQEARDLLQKKQIPMLYDDSGNNDQEMNVHAANRNAIMLNEVIPMIQNPYIIQQGKKTNQSIIAHKSQHQEMGEGKMISILAQPNATQSNYVQSHYQKPGLQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDX8</accession>
    <name>A0A8S1NDX8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0860235</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDX8"/>
    <dbReference type="OMA" id="ELCHHTE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="20"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="62"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="104"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="146"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="188"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="230"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="272"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8090838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="359" mass="39597" checksum="B0939A4535C2BE56" modified="2022-10-12" version="1">MPKNDVHLWDVKTGQQKAKLDGHSDYVRSVCFSPDGNTLASSSSDQSIRLWDVKTGQQKAKLDGHSSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSSDKSIHLWDVKTGQQKAKLDGHSDYVRSVCFSPDGNTLASSSSDQSIRLWEIKNKYCSDYRFKEIQVKNTRYIYYYGLPILQKRVFYKKPFPFRRIIGVLLVSNIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHC4</accession>
    <name>A0A8S1NHC4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0760002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NHC4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8086094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="397" mass="47623" checksum="FD978A96E27D1C2A" modified="2022-10-12" version="1">MLFDVILTDREASISNLGIKLIYFMDYHLDPQILAKLVGFEYDFNSKQVKISFSYLVTQEDSWQTKKGHEAQIFESVASFEYQTRFIDLLESIYKHQEKISHTQTLCTFCCKINFKATKGPQKSRFRQSTLNKKFKSKASYFSADRQYAQSKFFSGLKKRSNTYIYQVQYCYQNNILSTHILALQQAANPQFCIKFHANRVSQLSIKEKIKKLNFTQSHHIKILQQLRSDQDCVQTYSLLLIFSQKLSLLMLKTGITRYINDQQHNLYQEKFLFYLEFLMQILNMLLDSPFYLIYYQNYTQTMPEFHNQKKEKEKFQCIAWSKVIMNNKKISMKLLLKLKVWKKEYLEQLLKLKKRRRFKTVQDFFQIIQIQYIQKNTSNKKKNLEKVDQVQNQYMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHJ4</accession>
    <name>A0A8S1NHJ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0840012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NHJ4"/>
    <dbReference type="OMA" id="DIANFDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="665" mass="78952" checksum="7738123A7A49CFD4" modified="2022-10-12" version="1">MIKYFFGSIKLFRKQGIQDATKATTTKLDQITNPELTKDDIANFDQFYSNIVKSMVVKENGRKKVIQQTYTPNIYQILEQKIGNDHFQISAFLQFRRDQLREEEVVYCLDGLIRAKQFIQLKNSDYLQHIDSLISNKKLQFTKIECLEQILIINKQLQFLSIDSLRLIAKQLNEIGQYIEKEYELTKNEEPLEMLAQAFILAAQIEYNHTITQSWLIHVRKILKRHFKLLKFDTILKYFITIIKYQNKLQTELDSILDIEEFFGENYNVLTLENYLIIIEHLALIKYKKRSEVVIQFINNINSFTFTIQQLAQLAKSLAIMEIRLDELWKRITSEFLQVIQLVDAHFYLGEITLIFQSLLKINPLSPQDLTTIYKTIKNISIDESYTIKFQILYNFSIKGHFNFDLFQDLCNYEKNNFQFKYLNSYKQEKLFEVIKVILYNSIFYNKLECLDEKVDIAIQKALIILCHKIYEILSNPNYELKNSFQTQYELFIVSSLFQKYIENSDGLQETLINTKLENIRQDAGKKLDRKIQRNQFQIQEALKLLGFIDYFKVGYKEEAYQELVRMVQVKLIEYESDFDELNKLLHQYHIQNQQIMQEYTSLILNKQAELTIEQLQKYSHVLKKSGNQSQDLELIIIQRSLTNNSYLGVVGLDKPNYEVNFLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHQ2</accession>
    <name>A0A8S1NHQ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0900086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NHQ2"/>
    <dbReference type="OMA" id="YYFNVTM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035753665" description="Cystatin domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="14341" checksum="B91BFA4AAF31C60D" modified="2022-10-12" version="1">MKILLVLFFVITIKSERLLVSQDNGFVDDDNDFAGGIFPITDSNSDTYNKALQYAQKHYAESCKLNDLQWESLDSSKNQIVQGMLYYFNVTMKGNEKQEKYEIQVWIQADREQTIEIIQCKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NIT9</accession>
    <name>A0A8S1NIT9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0820138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NIT9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="337" mass="40029" checksum="31C7B91F97560A61" modified="2022-10-12" version="1">MIRQLKIQHLATNGEIIQVLENILSPNKITQKKTKEQSKHKINQNKQKQHIKSNLIALPYQYSKNLYKQMNKKQGQNKVYHFRIRVVFSRSSNQKIQQVNIMSFVIQSGEDEIKNKRKNGEKHLMKISQQNNLSTIQSNSHKNSQENTQEQQLQQIGSIFSTIQLNEYIILPVQYIKDDSTLIIKMMKYFLSIIIQILKNANLIKVEKNCQSDKKLIIQINRNQTLSRYYSELKELKINFLAIESQTRKQIIKIFSQDSDKQQQVSLQYINNKIDDIQQQEKRFIIDPFGIGQQINYYYSDKNYSKVIQLYKTQLKILRNVYIIQIYSYVIQIDNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NNK0</accession>
    <name>A0A8S1NNK0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0940214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8094078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NNK0"/>
    <dbReference type="OMA" id="QEMPTYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="114"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="27014" checksum="6BE02D453F9DD225" modified="2022-10-12" version="1">MSNIDQLLRSRPKILKGSQNLIQNKPTNEFSLNLDKALFTMQPAPRLIVPKEVNVNKKKYEGQIDQVDITEQEFEKLIPKKQKDSPITNKKIVNDKQEMPTYILEEEKTKQFVMEALSEELKRLDSNTDNLLRTLDQEKEKMGLMKQQNDEIEQQVKLMVQNKRLENRISQLEDQLNVPKEIKLDLLNADWNVANEKIEQMFKDNQELFNEADQIAHEFAKQQLEYEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NT25</accession>
    <name>A0A8S1NT25_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0920140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NT25"/>
    <dbReference type="OMA" id="QKQWNNS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="110" mass="13036" checksum="E300B8B6D30BC352" modified="2022-10-12" version="1">MTTNLGPKLKFVMPTKKSISLEDIFEVNFDLEQRQWNNSKMERKVSFASSTLIFLRYSEEEILHFRQRLKSQIHSSIELIESSYLNPQLCSPGKQNRKKSCFKENDEQFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTW5</accession>
    <name>A0A8S1NTW5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0920008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8092895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTW5"/>
    <dbReference type="OMA" id="KHINNFE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="226" mass="26413" checksum="D68F487535C77790" modified="2022-10-12" version="1">MNHVIKDNLIDLNLKLTDENQRLSLLVYNLQRQLDKAAVQINYAQSLEERLEIVYLELIQLRKDNQNLLEQLYLVSQTLISIDKEEAVQQEIQSVKILYEQKLQVLLKENKRLNRLNKELQNKQINNIQTHASKLMELSVELKNKLDSFGYQPSSLNSSQVAPKVLTKQIYDNLIQQLTQSSGEEKTFRIPKRLPVLQSKEIRIPKNNSHKNFTAIPFIQNINNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYS9</accession>
    <name>A0A8S1NYS9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0910046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYS9"/>
    <dbReference type="OMA" id="LKHKTIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="110"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="476"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="586" mass="68039" checksum="DEBFC6E6F1C2FDCF" modified="2022-10-12" version="1">MSNIQIENIEYPEDDESFVESDDSELNPFQFELDQQKSKDERVEIMYRALLKNRDNILEVISDANHNLKKLRKYASVLQKEHQIFNILKKAYEDLKHKTINHDQLLISLEKGYEKQIQELSNELQQLNQQIIQQAQEFEKQACLYKEQLTQKQEQLTLKEQEIIFIKNNELQLSDKLKNSQAELVKLQKESNAIKKIKTSNFLNLNEKSDFPFSTFELVIATKIKTQLVSFLSSKDIVNLCLTKKSIYFALCQFKVVIPQIIRCSTIQYQSQMKLLTKQVDLFKRLSNDCPEQIVKTQIIKYFDANLNPNEWLDPIIIEANNFVKGSQFLGRLEHIENADSTDGLKTFVQSMATRSQQTSNLLKSDPFNLSQFTANLIINSNDREAFLKERQNQLKKLKASQSDVQEKIFNLGTAVAADVGLFGLYLATMQRIFASLYVFGSYINIECRSQSSLIYYLAEQHWQLIQKQTILESMIQDRQQRIQNNSNEIKALNEQTKGLQDLIKTLQASIKAHKIETLNEQTEKAKIKQELQETKQLLFSYGKNIKLLALEIRELRKVNKEIVMQNNKAASQISDLKSQVAQIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZE4</accession>
    <name>A0A8S1NZE4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0920027</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8092914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZE4"/>
    <dbReference type="OMA" id="YCIVELK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14008">
        <property type="entry name" value="STKc_LKB1_CaMKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF77">
        <property type="entry name" value="SERINE THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="29"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8092914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="307" mass="36390" checksum="D65AE0872C174AA9" modified="2022-10-12" version="1">MNQQCKIFKSKEIVYQRDPNDKKKIINGYKVMKIIGEGSYGKIKLAIRDNQEYAIKKFNKFILKKKNKMYKNPNGTTKYVSLLDEVYREIEIHQKLDHPNIIKMHEIYDDDEREKLYVILEYAEMGQILKWESKQEIFTTPWKFDELTFKDYTKQMLMGLQYLQQQYVAHRDIKPHNILLTKNHQIKICDFGSAQQLTPQNDRVKGTEGTFQFVAPECLRENKKSDIPGYNGHLADIWSLGVVLYCIVELRLPFQSEIMLELFNQIETKEIKMKYDGPAKELILQMLIRDTSKRPNATQLLDDPYFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-09-13" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P127</accession>
    <name>A0A8S1P127_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1000155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035885476" description="Secreted protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="67" mass="7749" checksum="7532C54B541499FD" modified="2022-10-12" version="1">MKLLLFVLFVYTYATSAQYRQAMEDLNDDNFGQTLLETIQMHIQSDEPVGNLVNMLQNLQSQVEXLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P2H2</accession>
    <name>A0A8S1P2H2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1000065</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P2H2"/>
    <dbReference type="OMA" id="NVWVMIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF28">
        <property type="entry name" value="DIHYDROSPHINGOSINE 1-PHOSPHATE PHOSPHATASE LCB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="78"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8096044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="173" mass="20094" checksum="B78D39D273C41C23" modified="2022-10-12" version="1">MKIFIAATIIFILCLILDVIYDQQLWDVNTRITKYMQKQQTPGLQSMFNFFSNWINIFPGIALLMFIFTENKLASIIYMCLIQFTISFNSILKNVYHQPRPYWIESDIVALSCNKEFGKPSGHAMGSLMMSFLLPLMVLPNTFYKKPKLIKSIIICFASIWTFMTALSRIYLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P432</accession>
    <name>A0A8S1P432_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1040140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P432"/>
    <dbReference type="OMA" id="KIKITHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000051">
        <property type="entry name" value="Protein phosphatase 2C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="149"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8097634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="428" mass="48720" checksum="152A9190BF04044F" modified="2022-10-12" version="1">MFQKFGQQLKPIINRSSSENKKEQPIPKPNYHFTQKRDRSESRVQQSTVEDQNQNSLKPQSNLIQSVPAQRLTIKLPDLKGYQKQMEKSTSGADLTKKPPLLKNRLILDSKTKQQLQGQQSLLTQTLPIKSQREENQTTQQQGKNKIKITHYSQSRAGFDGIQEKTNQDRELCLQLDDNNYAFAVMDGHGMDGEYVSSFIKELLKYNLTKFYKVFDFQKVFFEMHQKMKFQTEFGCQFSGSTLTVLLIRENTISCGWVGDSRAILVKRNNNNNNLNVVELSIDHKPHLENERQRIEQNGGVVDTYHLPNGAPIGPSRVWARGAQFPGLAMSRSLGDTVAAAIGVSQTPDIKQLEIDNKEDIFIVLGSDGIWEFLDNQIIAELVYPFYQKNDAQGACQKIIQESVAGWKAHSEGIDDITAIVIFFQYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P545</accession>
    <name>A0A8S1P545_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tubulin-specific chaperone A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1060007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TBCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P545"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004226">
        <property type="entry name" value="TBCA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02970">
        <property type="entry name" value="TBCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="17"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU364030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8098015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="116" mass="13557" checksum="E0B376C0451FFE01" modified="2022-10-12" version="1">MAEAPLEKKIKVLMAASRRIQKEYLAYKNEIQQYENIILGIEDNEENALKLKKKNELLVESKSMLPNTFQRLEENNQKLMDQVVELGMMNEELTEQAQQCIDQTQLFLNQINPAMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P875</accession>
    <name>A0A8S1P875_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1080155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8099074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P875"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8099074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="66" mass="7749" checksum="B96542C53FC2446D" modified="2022-10-12" version="1">MNTHTISQPKPLIRLIQQIKTDSVSVSSTQSYDSHFSEIERPENPMIKNFIDNYEDDLDTFFDQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P884</accession>
    <name>A0A8S1P884_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arylamine N-acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1090030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P884"/>
    <dbReference type="OMA" id="FFWFRIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF0">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8099205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="35266" checksum="1D1DB57E7F5A4578" modified="2022-10-12" version="1">MQKQIKVSQEDFKTYLKGIGLPTDIKLSVSVETLYLLANKQLIYTFYQNTVLHLKDSKPISLEFSDILQRLCVQKIGGLCYEHELLFYYILKHIGFNVELIQCFVQEAGCPYDPEFPTTHAFLYVTIGDDETYLIDPGFGTRSLRFPMKVNFKDLDATYNLFPLEHYRFTENESHYQFQHLIDDKWVTYYHFFKPLKYATIEEIHNDYHYLFTYEKFSGVRDNRFQLCKYTEQGKIQFFWFRIEKKFTSFKKIFKFDEVKKIEYKSYEELKDDIKKELGFDLPEFHLVKSNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PAA8</accession>
    <name>A0A8S1PAA8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">STOP protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1100083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PAA8"/>
    <dbReference type="OMA" id="HYNQEYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8099673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="49341" checksum="02679FA8F7ADE131" modified="2022-10-12" version="1">MIPEHIIEKLKTKQGHYSIDVKESFIDKQRRLSEIERQTVLQKKHKEMQLFRQTFSSSNSKLSKPGKRIMIKTKGCSQAIPLEQFQQTISTPLPPTPSSNIGRKHHQSLSQSLPRLEQENNYITTLRKEPELQLNTQQDTHNQLEQQNQNMQTEQIEQMQIQTSEENNNDHIQCMVEQHLSDYQAFVMHLAGKCICVQCPCGKCKCKFEYQPFKPNISWKSHYNQEYKETKMKDQELKMNLNSIGRYQTLDLNEFKTTMASDYKQCDPFPNYVIEKQKYQANYGSTPMTSYNKFYMDYGDLHHEQFKQSHYKTVIPELKFNSSTTYGSEFKTIKQVDTTSQKPPNGKPFPTIDLFLGQSQNKLVHDQKIVEKCFQVKNFQEPIQTIPAFDKQFVSMTKKDFVMKEIPCIRNQYSQQQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PEL1</accession>
    <name>A0A8S1PEL1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1160125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PEL1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="127" mass="13983" checksum="505D59084B69FA10" modified="2022-10-12" version="1">MATALGLGYGTIAILAGFVLALLITFFFGRSTRSPELVCLLTMLIPIILIIIFTASPKKSQILTDSDQVDSYLPVDIFAMVFGIVSALGALLCILGHYYFQGLRAIRISSQLEMERDKQKLERKKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PEY4</accession>
    <name>A0A8S1PEY4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1140078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8101038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PEY4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="172" mass="20737" checksum="99F40ACF323A4DCD" modified="2022-10-12" version="1">MVLFIKDNIKMEQRMVEVHSNGLMVLFMWETSTKTIFKDKENIVGKMVENMLANGKIIKWMEKEFLLGWMEDDMRVNIKMIKNMDMVNLNGLMEEFIRENGQMENNMAKESIQVHQRWKKKENGKMVKQQGGQEEEINQQKSEIEIHFYNNFFTQFFGFLNICYYVLQTYFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGD0</accession>
    <name>A0A8S1PGD0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AP180 N-terminal homology (ANTH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1160140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGD0"/>
    <dbReference type="OMA" id="QFFRVIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="449" mass="52853" checksum="FE496E9BD4FAD84F" modified="2022-10-12" version="1">MKSFSNLLSSFVSKQYTPFLQKLNNCSLMQNTPTILMADILYLIECFEGFGPECKGIQPTGQEYLQQFFKVINNKNLDGFIGIKIILTIHLILYEFQIGDSIAEQMIKEGSVLHVQQNQQFSMFTQNYLMYLLKLAQNLKLFYACKIGQYPIFDQDLTQMQCQQNTRDQQFQQFQILNKNSNFRDTQLQYRNYQQKKLELNKQQSTGSITIEQKIIYLFKLHNLLNQCIQVLNICINTLKQLENQDSKNIFIELSCVLWNDCMLMYKFSSQELCKFLDSFRQLPSQQLLQIQQIYLVTINSSTQIKQLYQDRNYFDSQNIIKQPFWFEENKQLSQEIQNRIIDNKMQQIPDSTRKMNKVLTPQTQQNQISPTNIAQPFTSRIGNSEISTKTTIFSQPYFIANKKFQQQTKQLETQQSTNKRIKSHFGSIFSDEQLIISPKESSKLQQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PK54</accession>
    <name>A0A8S1PK54_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1210094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PK54"/>
    <dbReference type="OMA" id="NTKQRWE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="147" mass="17235" checksum="B46E73EB5B56979E" modified="2022-10-12" version="1">MSKLYDTNRKFNNTKQRWETLTANIGNKENIQVNNKIAATKTIKKCSTTITIEKKKPLPQRTITKYFKMTNLKKYPNLQLYDSKGNAREFLCYDDKVLNIPPAYNQIISHSMDNDCQSDNEQVESAVRQLTYFLESTLNQYKQQHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PL68</accession>
    <name>A0A8S1PL68_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1210106</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PL68"/>
    <dbReference type="OMA" id="YEWSEIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="16"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8103554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="463" mass="53358" checksum="26CA7932AA1B81DF" modified="2022-10-12" version="1">MNPQQQSKTKVFNNQYAIVKKLSSGSFGVVFLGQDIISKQDVAIKVEKEENEEVKSLDREVQIIKILDGVEGFPKYYWSGEDLGYNILVIQLLGKDLAFHFKQLKKFNLKSVLTIGIQAVNLLERAHSKGVIHRDLKPENMMLGVGNEISKLYLIDFGISKVYRDANGRHIMFKDQKSFLGTTRYASIAAHLGHELGRKDDLESLMYILLYFLRGQLPWQNMVNVTDDERTQKVGELKLSLESELFKDQVPEFQMIFSTIRKLQFKQEPDYRMIVQELRKAAETLNINIDGNYEWSEIKQSTHYQSDTNQNLSKQNIQFNNSNEMKKSIEKQLSGAIQHGQKDLLNMSSQNFLAPPPLSSRNNTFQRDDIRKNSSLTQQSSIGNYCQSLNPNYQKSICDDPLGSRQDILQNKQLSFDDEKISNFESNEIIENIDGQEPLIQKYEKVKKGIKINLIQSLRKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLU8</accession>
    <name>A0A8S1PLU8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1230072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLU8"/>
    <dbReference type="OMA" id="CWWNEYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20546">
        <property type="entry name" value="CYCLIN_SpCG1C_ScCTK2-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000375">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="1">
        <location>
            <begin position="17"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="261" mass="30568" checksum="37B690F907814DBF" modified="2022-10-12" version="1">MKLLIYEDWKNSASSRDNFTFQQEVKIQSECYQIARDFEILLKNSEQGTFNQYFQKALSIFHRFAHQVSYKKFNRHIYMAACLFLSAKDNDMFDWSVKRYASAFISYCISKKAVDIDGLKIENDDQNTYKIIQEKIIDAESHILQMIGYDLHIIVPQLYFQEAKTKLVVAQGDIQQLFEFAQKFLSDLFLNNACLYYEPKIITLCAITMASKLLKITIADLSNGEPWHSIFDQNLEANVQTQKEILEMTNYFDQCIQILKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQE4</accession>
    <name>A0A8S1PQE4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">folate gamma-glutamyl hydrolase</fullName>
            <ecNumber evidence="3">3.4.19.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1260052</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + (n-1) H2O = (6S)-5,6,7,8-tetrahydrofolate + (n-1) L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:56784"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="EC" id="3.4.19.9"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.9" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQE4"/>
    <dbReference type="OMA" id="EPVSSHF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034722">
        <property type="term" value="F:gamma-glutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046900">
        <property type="term" value="P:tetrahydrofolylpolyglutamate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000054">
        <property type="entry name" value="Folate gamma-glutamyl hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015527">
        <property type="entry name" value="Pept_C26_g-glut_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315:SF0">
        <property type="entry name" value="FOLATE GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315">
        <property type="entry name" value="PROTEASE FAMILY C26 GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51275">
        <property type="entry name" value="PEPTIDASE_C26_GGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035894519" description="folate gamma-glutamyl hydrolase" evidence="4">
        <location>
            <begin position="18"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2 3">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR615527-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8105104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="327" mass="37264" checksum="C95F7AE7FAF909B9" modified="2022-10-12" version="1">MPRILLLLSCLLFLSQSNFIDDIEYLTEINNNVVIGIFTQPSDPDYIDYPSSQYSYLAASYVKFVEMAGARVVPIPYEADNTILEKYFQGINGIIIPGGASELDTPTGPSKFTKAVAYMLNRALQMNEAGEVFPVLGICMGFQTLHYIISGYKTPFLYRVYGENGISHSLENGDRNFSLYKDFDDDTYQAMQTNQYLYYSHNWGVSPDLYKKYPSLDAFFRITGTNQDIKGQTFIASMQGKQYPVYGVQYHPEKNIFEWKVSAPHSYLAVKVSANHIDAFVNQARLNNHQFNAEQLNKAVIYNFKTVQEKNASFVQVYFFKNGELSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PT47</accession>
    <name>A0A8S1PT47_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L13a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1280094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PT47"/>
    <dbReference type="OMA" id="EVGWHYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1180.10:FF:000009">
        <property type="entry name" value="60S ribosomal protein L13a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3250:FF:000001">
        <property type="entry name" value="60S ribosomal protein L13a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8105758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="23065" checksum="5A7570A9B148D797" modified="2022-10-12" version="1">MFEKEIVIDGKGHLLGRLASYIAKELQRGQRIVVVRSELIQQSGSLFRNRVIFEEYLNKRMAFNPRRGYKHYRTPSRCFWKVVRGMLQYKSKRGAAALERLKIFEGVPPPYDTRKRQVVPDALKLIRLKNHRPFCSLGDLCASVGWNSQAVVNRLEEKRKQRGAAYYKRKIARENLRRKAIGAKELATINSELEKLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUZ2</accession>
    <name>A0A8S1PUZ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1320014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8106851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PUZ2"/>
    <dbReference type="OMA" id="KWNENNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039877">
        <property type="entry name" value="TMEM131-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22050">
        <property type="entry name" value="RW1 PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22050:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 131 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035899979" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8106851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="698" mass="81566" checksum="1FE656E3A27E3EB2" modified="2022-10-12" version="1">MFLFLLLYLIESRQIESCKQNMDSFQIKGIHFCVSDHHQAKEARNQVLQDSVSQNKVKAIVQEIESLDNQNPFWYSDLAIYLNIDEIHITGGLIDHSQNVSIVNQQIRITKHVYFKNLINKELMITNIFTSSPLLKVDFEKIVLPSNSTTQKTRVCTMIYEQPLSEIRSKTFEIWLAIEIAYFLRVPIKLIVYDKHLMCQTKQLGQCSIQGQHSTIEFNNLGIYQQYTKIFSFYNPNPVKIDVSYKQNERKKNQIRVLLANRNDIKSASKRKFSFEMQPYTRIELKVIINTNYYEILANSKLTFRTQYEIIEVDVTYSVQKGSVNYYPSVITLDPGMTTKVQQFDIFVRSTFSQKIQLLQVENQQIKHQNISLLTMATNIPTNSRSDILHVDYMGKQNFYSDSKYHKQQAKELLNSFGRNKIKIQTDVLQDSEILINYKSNQKFNEQILKKIIKQDPKILISQQTVVISEQPNIFFIVISILNMLYIATLLRNNVQKIKELKIVIEGNFSQEQIDFQSIFKTQESIQLQSKSETNEVVSDKADEIQDMIIAEDIMSKENIIYTNLQSQIDESSSHFSQGESLDDDQISEYQEKVVEQRKVSNQKNFNLFQRVEFTIPTQITLNTQSSQECWDTKDPIFLPQDSDEEMLDKNSVQRLESMLNQIQHKLPQFEQSTLKLPLKWNENNQINPYFPKPPPGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PVH6</accession>
    <name>A0A8S1PVH6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1330004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PVH6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="70" mass="8463" checksum="1F048BD8263FBB0A" modified="2022-10-12" version="1">MGNLLMHTRISLDQIQESISYQSYEIHTIIYVNFQLSSVINNIKLEYLFRFSQWRQEIHAKIQRHGILRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q150</accession>
    <name>A0A8S1Q150_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1390105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8109116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q150"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="60" mass="7602" checksum="F6360D1C1B8619E6" modified="2022-10-12" version="1">METNKIKRFIGEFNYKKNIYLKILESRIFDRMKPIPNEFYDCNTQLEFTEQQRYYFYTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q208</accession>
    <name>A0A8S1Q208_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1380105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q208"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035929530" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="432"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8108883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="56122" checksum="67DBBAEA44AEDCB3" modified="2022-10-12" version="1">MLFIQFLLLNLIHTEKIQKQEIKRYSIIHGRVSTIQIPIQDVFNVEIHHLNKTLNKTKEGEEPSVLFADFCQPKNPSIYLDHIQSINVCADQCKIIIQNNKIYSLLPGYLKVLEMNDEQGFTFFSETLLQISQNLTEMLITTNIQQDNKLIILDGVSLFIINIDENDEINKPIKLHTSIKVVTQIMYYDNLIYVFGNNTLEIYIIQEKLVELISSINITKFEGQNQNIFGVKLKSPNHFIWITESSLGYVQLDAQFQPIQFNTFMRLEGKIIDVEIFEDLTYVIQKLNNKINLQYVLEYEGGQLKQNLSLTTKVRQIFSTQNYIIILQENLIQLAFAKIETQQLFSKNILENTNKILKYDQGQLIVQQQNEIVQFEIKQQQAQLVCEPNQNYSTGTYMMELRTGYFVNETKAYHNQLVEINVYFEIFENGNVGLYVGLTIGLAMTFMTMIIFCVFFYKLKAKYQLVREQYHKNSTEFTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8A2</accession>
    <name>A0A8S1Q8A2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1470084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8A2"/>
    <dbReference type="OMA" id="IDQTNCF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="252" mass="29940" checksum="42820A7EBC61CA4D" modified="2022-10-12" version="1">MSNQTYIIRELIDKLSQQHKDYKLFKQLIKRQSKALESFNNEMIQISRQYNSDLFNGIHYHMSHFQSDLQNQYALLKELGKHFYEIKQEAQYYSKNYTKFELKLNSTSNAFDENSLKVKQTLQETTNFWAQKQEYSVIRINDMSNKGEEIIKKTNSLIYKFSEKHFFFIENYKTCSGRKSRNSSCNRTSSSIQSGLKSKETTSIYQDKSIDQTNCFQVGQSIQDFESNFNTLSIIEKEQSQLEDRRNLKKWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8N5</accession>
    <name>A0A8S1Q8N5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1460031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8110970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8N5"/>
    <dbReference type="OMA" id="IRVHIMN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="1">
        <location>
            <begin position="8"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="38300" checksum="8AC4CDE907B1D6F5" modified="2022-10-12" version="1">MEFQQQEYDVEFVHGKRYLNSFEYYIKWVGYSKKESTWEPETAFEGISNQLITRFERCQNYKNYHTKIYKANSHIKIRVHIMNKGCQVYKKYTSENKYTLLAQTNKTESNGQNYKLLQEPNNYKNYENRNDDKEDYDFFMRRGRGRPRKSETDREFLSYKNDYKIESQKYLHKQYPLQITECSFKYGHYKTEKEFIQEMLLMIKKKEQKKKRDDEVIIYKPQASLPSGYKSQYTDLPKFNLLNQKYYDKNSKCQVSDGKIPLECIISHHLMQNKLLVFKCKDNDEQVCYFDFDTLKSYYPTLLLDYLSITSIWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCH8</accession>
    <name>A0A8S1QCH8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1520076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCH8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8112657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="372" mass="44431" checksum="9DC94F3DE07D6959" modified="2022-10-12" version="1">MNNNSINYNQRSISQHTKGSPNYENFQTKILKQTYNYQFQVPQQINQFQNDQSRRECMQVRSNCNQFSRLQDGKQITYIKNKEEITDINKTYYEPKTIQNKNSSLNKEETRQIGIKMRSISEYGEKPVQNIQYQNLSHQNDNGKPQPKIKIISIQEKGLPIQQISYYPNRVNQAQTITCNEIKSEQQQKQKQQYQTKNSQISYVQQSNQINSVSESLINKHSQINQHSQINQHSLINKEPQLNLFETEIKTKVFDCYQCRQSSYENQYVLNCQHTYHMNCLKELIIGQINNFLQNPQITCLCKSKIHKLPFIDKSFYEEQQQKLIQKQLKFIYQSNQTKFRKCQQCDFFWVMDKYNKNNTLYYCKCQEKGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCK5</accession>
    <name>A0A8S1QCK5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1500059</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1500060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCK5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8112150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="183" mass="22437" checksum="C967BB5212188981" modified="2022-10-12" version="1">MKLNYFIDLSNHNKKIQLQVKNDQYQIKKIIDNQEHWFEEILEYYEEIVYEYNENDLNELNLQSQMDLYYLIQDNDIPEDCQEEIISLYEYYDTNDQTFNDLLDNQQTENKVNFDVQNFEEEESQLYDHEESLENDNLIKEKTINADNKQCSQGYNKQMKIKYINRTKIQKNNVKQQKKLKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QEV6</accession>
    <name>A0A8S1QEV6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1600020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QEV6"/>
    <dbReference type="OMA" id="IFEWFFF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="33552" checksum="E6808CD7F5C2AE9C" modified="2022-10-12" version="1">MSSRPDFAPIIQQWWKNLGFGQQFILFLQITVRFTDSFFVPLLYKLYNQTQLVQNGDYWRLFTAPLFSVSYIQIIINWMLFQLIVNPYETTVGSTYFIFDFFFKSFVLESINFLVNFGLSQYDINYQYTLSYGISGLTIYYLCSKLLASPYSKSNFYLFQITNYNLLVTIPLVTFLYTQKLIFIFAFQIAIFEWFFFDGMLIRLSNNSIEKLSQIFGQFKKYNSYKQPSQSLQEPKLFVESAKIQEGIQKEAVQEFGGRGITIGTDQEVERQWRAQGQNNYQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFL6</accession>
    <name>A0A8S1QFL6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1570109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000162">
        <property type="protein sequence ID" value="CAD8114024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFL6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="177" mass="21000" checksum="D71B643866D5C091" modified="2022-10-12" version="1">MNQRIRNQTQSQKSDAIFETDIDSQLKRLRLRNSKYVSAVNGIGFQDLLTKISENIPQSVHEEYQETKKEVLKLQSIVFYMKIWVFFRYHQLYLTKRIFLFIELNIRTSQSQKFVLSSNLFYKIYCANTTRSTLRFSNVIVQLIDLLGAFREVNLELVQEIVKQDRGLIIVLYNGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QI16</accession>
    <name>A0A8S1QI16_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1580071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000163">
        <property type="protein sequence ID" value="CAD8114180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QI16"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="640" mass="75138" checksum="A167E63C1035E557" modified="2022-10-12" version="1">MEKLKIFQLNLLANDLTKIDHILQYPNWNETNKQLLEIFQFCQTMKNIRYIESNKDQPENQINQNAVKKNILEQVQHQKINIKEFLIDKYQDQIEQRIVQQQFGLEKEGFKQQEFPQELEAQIWSGTRFIRTKLLINLQNNNEIQYILDGSIIRSDKIKDTSRRPEILTHLEQIQYLQWKGDFGNNNKKIGKWIATWMGNTLDGVGGYYTNDGKKYGKWKELFQNYWKEAQVYEEGEYDNGLRIGDWKYNYQDKIIDGGIYNYKGEKNGQWTELSKTFWDKSQIIYNGQYKDGKKVGRWAIKYEGVEIGGGQYEDYHPQNILFQEYDYKPTQQQNSQVQSQLSSANIKANDGIKNSNKIGKWIELSKGFYCNSQVTYEGQYRNGKKIGRWDIWWKKDYDDKIKVKIGGGLYDEEYSFKIGKWVELSEGFWDNSQVILKGEYKNGKKIDLWSIEAFGQLIGGGSYNKEGSIKIGNWIELCDGFYGAAQIIQKGQYLNGKKIGKWDISYREFENQPYIQIGGGVYDESASVKIGNWVELSEGFWDRSQVTFNGWYKNGKKVGRWSSYLKKNYGDFLDVNIGGGSYNDQDSLKVGEWIDVKDQFVDFQQLVYKGQYKEGKKIGSWMEIEIRKNQTRGIVNYDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJT7</accession>
    <name>A0A8S1QJT7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1650096</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJT7"/>
    <dbReference type="OMA" id="QVMFNDF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF1">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="85"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="361"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8115666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="374" mass="43517" checksum="EC45F6FEA9F1F849" modified="2022-10-12" version="1">MSKKDKDYTGFPGDCNQTQLKALADFRNIVNSMGLNEKVYDDPYLLRFLRARKFDINKTQVMFNDFIKWRKENDVDNIMTYMFEELPQVRTHYPHGYHKTDKIGRPIYIERIGMLQLNKLFEITSEQRLIKYYIQSYELLLKRIFPACSQAKGTRIDQTFTILDLKGGSMKMVSKQVYNFIQLASNVGQNNYPEILGKMYIVNAPMMFTGIWAMIKIWLDEKTKNKITILGSSYKDELLKHIDIDNLPDFLGGNSKCENTEALSLNIGPWNPDGTKPLFPVEQPPQQEEETQELTQTQEDEDQKQKLDQLKSALADMQFASPIEKAPHNPNKYEVTTQNNHMVSDTPLNTEVGEEDNQFSQQQQQQLQQQLQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKD3</accession>
    <name>A0A8S1QKD3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1640037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000171">
        <property type="protein sequence ID" value="CAD8115411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKD3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8115411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="39" mass="4873" checksum="8375ECE5A6D49244" modified="2022-10-12" version="1">MQENVKEISFQNRFQINYIKYGVIKQKVINEQYIIYIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKS8</accession>
    <name>A0A8S1QKS8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1700024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000179">
        <property type="protein sequence ID" value="CAD8116219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKS8"/>
    <dbReference type="OMA" id="CESQIEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="98"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="364" mass="43375" checksum="41F0D2448519EB7B" modified="2022-10-12" version="1">MLADKTSAYVNQSSVQSKQPSKRVQLDIQYVAQTFEKTQSINNLQDMFPDNQSTELRNSMTNEESEIEKLSQAYADKYYYEMKFEQYQNLLTQQQGTITKLLNEKKDLLARLESAKSNEKEISLLQQLEVKFIESQKEKQQLQMECQQYKYQVEQLLVANNELQQSFQSLHNQFVEYKDKQDKYSIKQQELIAKLTDDIGNQKYLQEIHSQMQNIKQQNNQLSEQLSQQELNFQIKLQEIQEKYLIQSNELTQDCESQIEQKEEELHLLKMEYEKQKSETQKLHNENMQLQNQIQQLTQNYQDDIQAQASLLSEQVAEYKIVIQNLENQLYQMNQEIKQRDRSSSKDSMINSRLISNYISRYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QML9</accession>
    <name>A0A8S1QML9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1850010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000194">
        <property type="protein sequence ID" value="CAD8116963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QML9"/>
    <dbReference type="OMA" id="DYSSNCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035763686" evidence="1">
        <location>
            <begin position="23"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="496" mass="56855" checksum="BF57D11600B8DA50" modified="2022-10-12" version="1">MKLNFYLGFLQFCFVFSQPTWSTIYSLLPGLNGTDSWFKNHLYGGPISSCSGYDSFGGRRVFGIKQVFIKIECWNGESFQLIYDNDAEIDKFGSENDCNGESDENQVQPFIFTLQSHFSQSLVLIMTTNVNTEYVYILQFQKPWGIREFKIEIQECPEGCLFCQDYISDCKLWNHIASYWQNSINSEGWLIDNNQLLTSSFCAGIQIAGGTNILQQGQSIKKVIQNVPNHFKIQIVLKLWVMGITQSQNLNLKIGEQGWYTKIDAQYTLAIECVNSQQVNIYNIALNIDHSSPEIEFKMFIENNKPQIQFWGVSSFDLYVAQCSIGCEECNEGLETQCSNCLKKWGLKDGKCIPAPPLEYTNIRIYQSQGLKINFINSFQLYIQELDQTITTIGQNKLIIDKSIIFSSFLISVKCQDKMKIESFFRNCNQCDNDQISFSNYCLNESNIVNFSVIFADTVQSEKELIINISQTKVEILQVILQNGIETEVQIMKIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNI6</accession>
    <name>A0A8S1QNI6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Aurora kinase</fullName>
            <ecNumber evidence="15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T1700037</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="15"/>
    <dbReference type="EMBL" id="CAJJDM010000179">
        <property type="protein sequence ID" value="CAD8116245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNI6"/>
    <dbReference type="OMA" id="LAHPHIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001241">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="17">
        <location>
            <begin position="10"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="91"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="169"/>
            <end position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="218"/>
            <end position="219"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="11">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8116245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="474" mass="56143" checksum="637C3BFED310DA39" modified="2022-10-12" version="1">MNRFCDYKSCQYFIKQPTIEQRSKLNKVMCPLCMGANYCSTRCRDLDWPIYHKEICKKSQPDRARSCNDTIDSCSTTSVRRKPEEFEIITIGNKQELGRGSYGSVKLVKDKQNGLLYAMKIMNKRQVFEYCSVENLKREIKIQRKLAHPHICKLHHYFEDKENVYLILEYAQNGSVFNYIKKRSKLPENEAFVYFFQTCLGIDYLHKNKIIHRDLKPENLLLDQDGNVKICDFGWSAESLTEKRMTFCGTYEYMAPEMLNKQPHDFSLDIWSLGILLYELLHGNAPYRGRNNEELGNKIKSGQPINFASTLSTEVITLIKGILKYIPGERLTMDQIFDHPWMVKNATNYNIDIWTFVYKTQYPTRQIPTANFQQKQKPIEIQKKEILARPISQDYNKLKEQQIYNNNTNNYKAYQNEYINKQEDFTKPRISRVSNRNEIMMHQHQLQQQQQQEEKLSFMDRVFLAFGCLNREKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQR4</accession>
    <name>A0A8S1QQR4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1850012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000194">
        <property type="protein sequence ID" value="CAD8116967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQR4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="109"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="337" mass="39413" checksum="1632890C21DE858C" modified="2022-10-12" version="1">MIQQQQNNQSKYFSHKLLYSITQKYPKHCFAQAINNNNTLLVLGSSIVCKIIYLKNELKQLQQTKMHKGAISTLNFFKSRQQFISGSYDSSIIIQPTNLLQNLKYITKLQGHSRGISCLVVNPNTEDLIISGSVDKTIRFWYYSSSSNWSCSQTINEHNDQVNGLSINQDEINKFYQWKDLMDNCGILNKRFLKMVIVLHLQQQIYLHFNPVMVNIYNYIQLIIPLDYMQNQKEPQVKGVGSDCQYYFPQIYISSRKLLLSKNGYFVNLLQFNFDSENWDCEFIEGIEFDQRGNFGNIFGTMSDNGEYLITWDCMAGNIEIRQFTYKNDMNDHQIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMS1</accession>
    <name>A0A8S1JMS1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMS1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035941768" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="21475" checksum="BE4C5D4249DCCF3C" modified="2022-10-12" version="1">MWLVGCFATQLFLGYTFFQGTSSFDQLSNILEMISIQFITNIITNFQFIQHSPNVRIILLQNQKLELKTLKMYEDEFNIDLLDPKILNIYNFQYLYQESNKSSLRTIQEMGTMEDLVDLLKKLLEFHSALCSLLANVSNIHFQEIFNLILMKLQNKTSNNNDKQINYNILCDNIFIVYFIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPV6</accession>
    <name>A0A8S1JPV6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PPRIM_AZ9-3.1.T0080107</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPV6"/>
    <dbReference type="OMA" id="GNCCATT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000299">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000383">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000828">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 27" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="27"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="330"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="366"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="401"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="437"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAD8044543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="473" mass="53991" checksum="5E37F3C5817BFAA8" modified="2022-10-12" version="1">MNQEEVFFKKQWFILSHQGKIEQFYDINLKKVIGSGTYGSVVKATLKGTKNQRAVKVIPKSKVKNPDKFKKEIDILIQLDHPNIIKLYETFEDQRNVYLVMELCEGGELFDRIMDKGCFSEYEAYEIFLQIMQALNYCHSNGICHRDLKPENFLFLTKADDSPIKVIDFGLSTLFEDPITSKQTGQKLTMKTKAGTPYYISPEVLKGSYDELCDIWSAGVILYILLSGVPPFYGDSDPEILDAVQKGDYTTEIPELKAVSEAAKDLIKHMIISPDKRYKASQVLKHKWMIESNKPTKQLKLNFAQLKNFNGSNKLKKVALTFIASQLNEQEITDLGKLFKQLDKNGDGVLTIDELREGLIGVSDSQQKDLATIIRSIDTDGSGTINYTEFLAATMEKSLYMKEEKLYQAFKMLDLDGSGKIDKQELQTVLGKSEKVIDEKYWDDMIREADKNGDGEIDYNEFIEMMDKFSLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JR60</accession>
    <name>A0A8S1JR60_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JR60"/>
    <dbReference type="OMA" id="CAREHKG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="522" mass="61012" checksum="7BFE2A9491EA098A" modified="2022-10-12" version="1">MNQSSIFGSVTSPNPESQMNQPSSQLTQSQQIPIPPSSGLYMSTPMPPPVDPLLISQQIQSQMIQPNSSIPQQQLDTTYVNQNQNNQFYYPYVESQTTEFQKVTGYNNGSQIIYQPSQYNQSSYNYNYQQQQQQQQQQQQQQQQQQYQQQSVIIPPHQIDQQQKNIQEFSQSSIIILNKQDSFSTQSSQILENSKIISNSKIKEMDISSTTHSMIVIEEKTNEFLIQPFDEKLKKEKQKNKQLVNSNFFIIQNNNTTIPEFQCQERLCQLHSKPIEKICAEQDCKENNDLLLCQDCARDHKGHNLFDIQEALFLILKSQNNLSYDDEVIETIKTKTFQQFKILKSNITWQLKEIEDQLQEGFDKFKQFLQEKKSSYQQAFNDLQFGGLSKMIEQTNYLFDQGLFLEVNLQSNESQYQQLAAQLDKVKVSMSRFEQLIEDNKQEHVQIKLNNAELLIESQKIQNLNDLYFQIKIDALKQKFTNILDQELLILQDNKAIRLKGNLEEINLKDQQNIQIISYQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVB5</accession>
    <name>A0A8S1JVB5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0100458</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVB5"/>
    <dbReference type="OMA" id="CKNQSIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47026:SF2">
        <property type="entry name" value="FLAGELLAR ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47026">
        <property type="entry name" value="PIGMENTOSA GTPASE REGULATOR-LIKE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="107"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8046563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="282" mass="33630" checksum="BE7FE85044075D32" modified="2022-10-12" version="1">MSSFQRPTSSRKNTNTLPAQQGDLEEYIQILYDHQKSCEKAGKYLEADQAKKRLAELKKELDSKNKGDVKDRHCSEKQEIEKAHCDEFNQFNTFWDQKMMEFDSEAQKVKEQTLQRHEDELRQFSEELENSIPVKPKDSAELLALRKTEEQLAKQENYMEAHLIQQKILASERDELEKWTVGRQQKIRNLISQLRQKQINELNALTQRILSGQEEQRKIRSQELEKLLQKYQNVRKELESQQVQEMTRLDKSCKNQSIMQQSRMNQSRMQQSYAKDENQYIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVX1</accession>
    <name>A0A8S1JVX1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CDC20/Fizzy WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0110099</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVX1"/>
    <dbReference type="OMA" id="CPWLPNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF8">
        <property type="entry name" value="FI02843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="7">
        <location>
            <begin position="77"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="203"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="336"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8046782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="390" mass="44521" checksum="2133B6AB2954F4A1" modified="2022-10-12" version="1">MDRFIPKSKSKLLYQSHTQSNYNDLLYHINEEKVLNFGNQKQQHNYPISFLDQLHNQYRLPTQQTIRQISQVPEKILDAPDIADDFYLNILEWGNNNVLSVGLQNKVYLWNASNQHIEQLLQATSNVTAVHWINDHILGVGFDDASIKIVDVCSQQTITQLYYHNERVSTMSSSFELLSTSGRDNVIFNHDLREKNNNVVGVIQKHTQEVCGLKWNQSGTTLSSGANDNQLLLWDKRQMSLRLSCEGHCAAVKAMAWCPWFPNILVSGGGSNDKNIKFWNSDTGLCFKSIDTGSQVCALQFLPRYRELISSHGFSKFQISIWNAEVIQQAKLIQELQAHKSRVLHLGISPDQSMLCSAAGDETLIFWRLGTEQNNQDKQEMCSSKNLFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWP5</accession>
    <name>A0A8S1JWP5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWP5"/>
    <dbReference type="OMA" id="KSEACMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="9"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="37388" checksum="0B4E33DD549C5D57" modified="2022-10-12" version="1">MSTDNTELLSKFLNEYNQLKKQNQEYQQKENSTYQSLINENQLLVQELNRIKKDNQRLIDNNTFLTDQLNQLKELYQETKQELQESFNFIQTINKTMKTGTFEHDDRDINKLMEIFSVNSMEQLLITAEKIKTVMLGVAHLESFCRQICEIIYDQNEEQYNLEQVFPIIQKWKFDSKYVDCFLNFKNQLEQTLSLKQSTDSQIIDAIKSLQNTSNNDIQTIKSLFKIESSDNLMFKMNQTFILLQDIQQFIKIAKRLLELDENMKSEACMIYILKIIEKIKQNQFNDPDLILKIMKTLKVDHPSQILNKLEQCTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXS5</accession>
    <name>A0A8S1JXS5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090445</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXS5"/>
    <dbReference type="OMA" id="NIFQNYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="211" mass="24991" checksum="5A194FDCD915E633" modified="2022-10-12" version="1">MYQNIFQNYMNTPQRQYIPQCPPQYYYPVQNPFYPQYSIFQAQPVLIIKKKDDQTKQEENIVQTKPQKTVSIVDFEKTSEQIQTPSVQIQSDDKGSNLKPNLLVDSTNIQKNFSKAIVQYALRQKKIVYEILGEEKGNQFLQFMSELVNQLRNLFHLVKYTQDEEYLKAIRILGNNFLRKESTCYIYNSKIRQKTSHIKHKAIVKKVLLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0I6</accession>
    <name>A0A8S1K0I6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0I6"/>
    <dbReference type="OMA" id="NGCYEIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="445" mass="52791" checksum="ADD21D9ABFD1F8EC" modified="2022-10-12" version="1">MNSNWQNEFNWIHVSGVLISQGNENMQIDLVPQQKDVLNELKIEIHPSVSIKKQLNYNDETGWSWHKSWNLPEIEYNINSLPNQIKLQQGTLFVQIFAVKALEKPLQYKNLGIKGESKKQIIDQQVNFRCLKFTTTSYNNDHKKFHLMIILLYSPVNDQNIVLSSIISPEIYVDSRKFARFHNIPIHQPSFTELFHYDLLYSIITKRETKNKSVKMIKIENSIIGFINYFTASNIRNKIKHPIFLALRFSMENQKKIKLLISLQNEDNILQKKAMELIQQLENGCYEIYTQQCYLPANIIQIEDLQQLILQYGELYQKSQKQNSKNKNSINTKTQSQSQIDNIVVQKVKIEQFPNIDNKPLQTTTYQFENSVKKEEDQYVQQQIQQNIYQQYAQNANINQSQLYFQSYFNHTYQNYMTVQNSQSSNQYFYPYPFTSSQYIQWKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0M8</accession>
    <name>A0A8S1K0M8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0M8"/>
    <dbReference type="OMA" id="NDQQFSY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="170" mass="19618" checksum="BE10DC1C6A102F56" modified="2022-10-12" version="1">MGSSFCFQPSEKSRDELKLITNKQYKLNGPPHLFKPTPIVNISIFDEQIEKEESPQIEILEQPNNASNDQQFSYIAEQSEEIDSSQQNPWIKKTYAIETINVSNLKNDENNNNKNNISQQTNFHKENNIQIIGKTKNMKEVSIISIKQGSNTNVTYDKQQLKNKTQNKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7A0</accession>
    <name>A0A8S1K7A0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0100302</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF-assemblin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7A0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008374">
        <property type="entry name" value="SF_assemblin/giardin_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06705">
        <property type="entry name" value="SF-assemblin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="151"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8046338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="250" mass="29651" checksum="47F2DB4E5E54EEB6" modified="2022-10-12" version="1">MSESNVKGRVQAIFEKLDNISTSVQDEKNSRFHTISQLIMAFEAQLQHNSEQKEEKFNYLASRMRQLGEYLEQEQEDRIRQEAETQKLLTDLERHARKLIEQNSNQRVEQERKIVYQIGQQIDNLQGEVVKEGLSQSESYQFVDAYINEDLPKLADQLQNEIGERKEVEEKIQVQFFQQLTDLKEAFDREKKEREAKEEEIVESLREISGRISEALKKTRTEREKTEETLVQLVEKVVEKIKREMLEMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB33</accession>
    <name>A0A8S1KB33_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0180332</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB33"/>
    <dbReference type="OMA" id="RFQCIDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8051787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="174" mass="21091" checksum="434B784F2D78D9CC" modified="2022-10-12" version="1">MLEKMIKFQVVTKEDKMNSLQPLLPKIQFNISDKQHNYTETIDSIGNRQCGSYRNYSQKRNQIKSFRRSKIVGYSQLINNYKNASRQSQENPFSEELLIKQLKNNRNQEQFSERQYTVMMDYQQNKYHTIEATTKNIRYKSQPKKLECNELDMKITPYIHQNKRFQCIDYYYII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB64</accession>
    <name>A0A8S1KB64_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ran GTPase-activating protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0170286</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB64"/>
    <dbReference type="OMA" id="RRVEMMA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8050895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="39282" checksum="4615E2D31A7B996E" modified="2022-10-12" version="1">MEQTYTFTIESKKQNIDALTEEQQQEYKAHAEECEIIVLKGNSYSKQFFNQFSEFITTSKKLQKINANDIFVGKGKDEIPQSLQILGNSLIGLNILSLDLSNNAVNPFGAEALKPFLKQAHQLQRLFLNNCGLGIRGVTQVSEGLQEGEHNLQILAIARNRAECDGAIEISKAFPTCKKLQELHIYQNGIKQKGMMELLSSLNKNCSELTTIDIRDNFVHEATTQVLSDLISNSTHLTAINISDCNIQGKQNKQILESLSKLVKIERLGYNYAELNDVQGNELYEIIVKQVDNVSKLELKGNEFKKATKQKFKELFVNKEKVLGAFDSDDEDEEEADELQKLFSQLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCP2</accession>
    <name>A0A8S1KCP2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0190095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCP2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="30395" checksum="3B550A347F37870F" modified="2022-10-12" version="1">MRSMMKNYQDTKLKGIGMYFLNFYFYHMLFFILLGPFVVLFQFKYPGIILLKNMRFLKHSSSFYMQTLLWMGSVLGGLMYSIKNKSVATMTEIIFMWLAITTHSVVIAAKYATQCTARIKLYKTQELLDEMLSFDLMFQDWFCQSSKVLFLEQYRTLRRHEQECTLYKFDFLIEPSIKKSEAIKNTNISFYLEWAIKQQMKQTDPIIQYVYYNGFHIFGYLANQFQQNNLQSSFYKYLISLFYFMESLLFILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCZ0</accession>
    <name>A0A8S1KCZ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCZ0"/>
    <dbReference type="OMA" id="CFHIIMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="326" mass="39499" checksum="9F58F0BD3A88BA3C" modified="2022-10-12" version="1">MFKKIIQGFSKYSHRSTIPSLLKDPRYTRRGISKPKGETRMLRKPSIPDDIPNAHLYYDHSKFIEIQKNNPPHKLKYESTDLDFILSVLNNDSFIHTQDFKLWKAIKQLNIHHPNEVKKNWDIFDRAYRVILDNARYLDIYNFRSFTQVSQQYYLEDEKVWTVLYRISIQFFVGWEHQQTPLLSKLIEKTYQQSFLFCFHIIMQQVRKFKIDVSNIIIHLDYLMRNHLRFYKIHSEETYNKVENVEDKNQLGNFIAHFAFCIQQRKDIFNEVDKEIKEKQVEEFLHQGQKLCLESFDKLTAVTKKNLQNSITYSRYQYKPLFDKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHG4</accession>
    <name>A0A8S1KHG4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHG4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009563">
        <property type="entry name" value="SSSCA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051888">
        <property type="entry name" value="UPF0148_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16537:SF1">
        <property type="entry name" value="PROTEIN ZNRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16537">
        <property type="entry name" value="SJOEGREN SYNDROME/SCLERODERMA AUTOANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06677">
        <property type="entry name" value="Auto_anti-p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="320" mass="37240" checksum="11C74D1D829D1B0B" modified="2022-10-12" version="1">MSQFQRMNRKLDEGWKLTTMVCECKATLLIFLENRNLYCPKCDIITQDQVILDQDSQDTKIKTNQSGSESKPEIIQKKNSKDYDSDDFNFDDQVEDFNQRFLLNKAAADEASKKIGQLLLQGWTMLGESCIVCLQPIMKSKQGLRLCVQCNKEERIDQPKVQEVKSNITQEKDTVQMVVQQVQQQNIQIQQKKQISEPAHQPQLEQKIDNIKYDISNITRSKEDAQTLITSQLKIYYEKLQSSVDDINFYNLNFLSEQINIIEQQVSQTLNNNTELMSTRINTLKILTSIHEKLMQQNSLERAQKFMGIILIIYNIVQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHH2</accession>
    <name>A0A8S1KHH2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0220133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHH2"/>
    <dbReference type="OMA" id="DSERHVF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="82" mass="9421" checksum="DA8D9EDA2CB063E6" modified="2022-10-12" version="1">MSLHSSFGYDSLRSVIFARAIKECEKIQNEHYLSENGSDIKSSISSDSDKHMFDREYSLESAESQMTFNNQQSTLSQFNTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKE2</accession>
    <name>A0A8S1KKE2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0200270</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKE2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="129"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="351"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="664"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8053322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="782" mass="91638" checksum="1876A61586120FB7" modified="2022-10-12" version="1">MIFDSEIQIVKEIMEYLLYYVSNVLFLQREEFRLAFLMLEMQLKLNRRQYCFNGQNCKLMNQRKKILNEKINNINFMFQIKITMLILYIVSCFEGKNLIPLCINIGLICTQFGSLKFQTHTIFQIYETLILFIQIALITLYNQQMIIYNVIYIIVLDETGRFKSCLFQKVSLLGTSVFIIVFTSTNVIEYLRNSAFALGIIIVMINKGIPKKEEFNNLFSSTRTQSPRVYDTQGLMIIDSQNLNCIYMNEYIKRKLGDQQLIINKIDSLSKKKLDQKIQNIELFELLNEFNLKQLSSMQILDSNIMADHIIQVTRDENQNKINITFQNSKERDDYLKKTYLYQILKQIFNTISHEFGTLLNFMLAIAQVAIDKYPNQQYYYSIRNTCEIMHNFIYDMVDYNDILGNQFKLQLEQLDIQTILEQVTTLFQEQCTQKDLQLDFENHIPESWQIISDQRRIKQILIHLISNAQKFTLNGSIQIKVMAINDDLIQFSVTDTGIGISENDEQNLRNMLEQDYKDEQKLAQNTAGFGLGCYLANKICLHLSNKQFKLEFKRNEVGTTFWFQVQNNVLPTQSIGKILGIQQINKHSYIDLKQSQIERTVGKKLSRVASKISINSKSRVVSFRCLIDPVKENTESVINEEVNSIERKVRTCTLNRNIKKSIKFLIVDDEIINILGLQLLLKRMNIDIDYVFNGVECLRQIEQKKHIYQGIFMDINMPLMDGYETTKRLISLYGNQIKIIACTAYTDSETKQRCYDIGMSYFLNKPVNIVELQKILIQFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKU9</accession>
    <name>A0A8S1KKU9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0220353</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8055011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKU9"/>
    <dbReference type="OMA" id="HADVCIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000011">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="15">
        <location>
            <begin position="48"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="16">
        <location>
            <begin position="247"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8055011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="449" mass="50901" checksum="660147B34ADBADB7" modified="2022-10-12" version="1">MKEIITIQVGQGGIQVGNACWELFCLEHQIQSDGQMINPQVIGINDALKTFFYESDHQKLVPRSIFVDLESTQIDQVKTSKFKALFSQNQFISGKEGAANNFARGHYSIGKDYGEICLERIRMLADNCSSVQGFMMFNSVGGGTGSGLGTLLFEKLSDDYNKKSRLSINIYPSPETSVSMVEPYNSIFATQSLLEHADVCIPMDNQAIYDICKNSLDIETPKYSNLNRMIAQVISQITVDMRFNGALFTDISELQTSLVPYPIFKFLISSYAPIITHFKMDHVQLSTVEISKLAFETENMMVKCDPKQGKYMSLSILYRGDIIPKDVSYSISQLKNQKTITFVDWVPTCFRVGINHKVQQTLQEDDICKALRSACMISNTTAITQIFSKLCHQYDLMFAQKAFVYWYLQEGMEEGEFVEAREGLASLQKDYEQADSEIFEEYLKEKTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLP6</accession>
    <name>A0A8S1KLP6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0240156</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240157</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLP6"/>
    <dbReference type="OMA" id="AIACWEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8056128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="217" mass="25772" checksum="32F9A5E39DF7A18D" modified="2022-10-12" version="1">MQINQEFLFEELASQNNHQEIIDFCDSQISSIKFSQINSLTNQTHILDYSLELYKATEQSSGNQILSRVDSFVNKFQIEHAYEVYYKQTIKSLKILQRYQEIIEYCDQLQILCQEKNQEIYCQKEKSQQIRNKHNMNYYLQMGKEYLNIIKNDLNLIEEAVACLDYGMIQNDSFQEQFNQERGSKIINICLEKLLKNNEKINEAEAYQKQLDSLELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KP01</accession>
    <name>A0A8S1KP01_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SUI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0250146</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KP01"/>
    <dbReference type="OMA" id="CEFMITQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="3">
        <location>
            <begin position="22"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8056969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="12469" checksum="586B02D459C1CDD7" modified="2022-10-12" version="1">MTDNQFDNQNEFDEMVNFNFVIHIHLKKRSARKTQTIIVGIPDEFDLNKIIRFWKKQFNCTGGIISKDEDYGDQVTIRLTGDNRQQIAKFLVEEGIALQDNIKVHGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQE0</accession>
    <name>A0A8S1KQE0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQE0"/>
    <dbReference type="OMA" id="HEKHHRQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="726" mass="86630" checksum="F5EC2102F2951C98" modified="2022-10-12" version="1">MLLTNNSSQNEIQEIIAYSQSNLSIMLNMEQFLKYLRENDPFAIDFLFKNVQQMFDILFVDFDKVQMDQFVLYSDLITKIFEIIDSNIEPIMQQIENYWEVIFDLRWRKVTPDIQWGLAHRLLQLIKERDKKFVIDQFQRPNFLVSFLPSLQIDSVAQIIIIFYEFGFHQQQLDFLKAGFAIFQDFEPCKAINFTYIVHEIMVRILINEQIEYIIKGEVQRKMLSIIVRDDINSITQKNAAHVLSLISYHYSKNMQNIQLYEPETLEITQKFRSTEFFEYFDVKILAQILNQSLLYQTKVGFLTIKLIEIIDNLIRITDIQLWERIDKANIMELILSLMHKFNKSDILISSVRKMISFIYDRALNDYHPYWASKLIFKNKIHEKHHRQIDYQIYQFEQKLSLKLIKDGDFLLYFDDLIQIEEELMVSHNWRTSKVQQMKKEERHKNKLGEDPSSPQIENNIIVSAMDVDDFKYQEQDGINESTNNIFINCQANSNPKQNQQEITKNLFETIPIITAYVDSETNSESSHNENEIQNDKNVILEKNIQAGQYHFEDVLDHIDYKLNQSNKLSYSDQLDKIIKNRKLSNNSLNQDKQQNKVMTNSLSNEYDTSFLKHQRRNSLQPVPDINKFNENFEEFHIDFLNDRKILKTDNNCILQVDEIDLEKESPQKFMQIVFQKQNKEILFRTKIVDQINLNTGKKIEKVEDIKFRQIQEKDLHFLDFLNNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRV6</accession>
    <name>A0A8S1KRV6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0250285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ISKFQYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1067"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1238"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1343"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1565"/>
            <end position="1585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1628" mass="193248" checksum="C1E10BBC782309CC" modified="2022-10-12" version="1">MYARPLLLFNKFFQNINLDFNYDNTQIWSEIFLILMLAMQLLYPFCFIHLYCSYRVKVQNKQQRKQYKLFKQREQQVIISLTQNEINSIINVIAVSEIYITFATKIFKYIFHYPIIQLALRKILTQSNQYSNLNTYQILILIITYLVFILNILFYFLNESHDVQFSFKKLDYLSRFYSNKTKIQSLKIEILIILVSILDNLEINNIVLIIFEIILLIIILTLSYRYPQYQETLINQIQIQSFSLYLCIIFCILIFAEGFHQDCSELILIIIPLVFEITNLIFIKQENKTQTLSYENKNFDYLMMTIYNQCQEALGRNGILLNEIKYRLPSNLNLYAFSTNHLINCQKLNGCFCTCYKKQNDHFITRQQFKQYIKELIRFHYELKLLTLNKNNSQDYAKSQFYYILYISQVIKQPTKAFHEIIKLKNNMYSNLNLLDQMYTNSIELLVKSDFLKMIENKNITNQKYDCFKVFYYNKCINQWKQQFSQVLQKQKQWYVTLLNRNLTQLIIDGKHLQNLISIQEKQLKYIFNTNPLSQQCHILIHLFYKYINFNKKRIEIPTIDSSIAFKFQNSINGILFHKEASLVYLTLLDTKGIIKNYTKTFRDALCTIDSEIMNNSINNFIPDIIAEVHDQYLDNFVERGRINIMKSDRRFLLVKNKLGFIFPIIAKVRLETDLGSDFGSSALILPTYQNYHYIMLNKYGLVEEISDKLYNEIILPILCIDLKSIKSLDCLKLIPKLIKSWKQLNQTDVLEQELKNPIQSYMIIPKKRKKQQILLSSQVKEKSTIYEYLKQQDQINHNYFDNFKYMYMFRITFKIVEFHTFKETIYCIEIQTIKSVRESQRIEIFEKLIEKSDYLNMSRKIIKLSPDKIDIKKKKCKKLEITNQKGSLGFISFQPEQQTIYNLETQKLIEDDYMIKSKEMQIRLTNPLYILEIEECQNQISHPNSQDKRLQDQQTFRMKSSVAHSFGALNHFCSSNSNGISQAFGSDQNKLIVHSSAILDALNENKESVKSKNSDELIGSIDNFLFDQDQMKSISSSQISDIKVKKTQLKHNLFDEQTKQHWTIRLFNLITFALLMIFNIYYYYVLKQENQIIEQALETYKISNNFQVQINNFILAFNYSNNNIFNYTLYMKFCANRFQNDISKFQYYSLNIEQNEIPYLQINEFENITNLNLFYSLGYFSQYLLTQSNCSNQEYFLEFIARNFITIISSNSVLNQTVIKNGLDQFIKAQQSTKVSFYFTMISLCFAFIFYLLLLIIINKAKLKIVELFNTISKGQINLLIEQISIIGNFLEKIDFINEETTEQQFDQIKSKFASTPGIRIKTSQKRSSIKVKYNKSQENTAFTYFIYSLLLLFSYFIISSQYVGSIIYQSSFEQQTIYSFEQLLLFKSSQSYLLQQKSFQKLLIKYLQINKEDMLFLNKLEEALQYMEEQFDILQGFIQRLNDQKSFSNAIITNTIKKNSCQAFQFTIANNIEDSQFFNEMICNNIDSLTSGLTISLVEILQNFQQFYTINKLNDMESKSYLFNMDINLENDIINVLYTSHVLTLLHDFMAYEQQQEISLNHFVHTLRFIFGIILYLILIIISDKNIRMYLHKELIKTKQLFLLIPLEILCENANVLQQLIIRNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KU25</accession>
    <name>A0A8S1KU25_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">VPS10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0250077</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KU25"/>
    <dbReference type="OMA" id="NIDPPAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.80:FF:000007">
        <property type="entry name" value="Sortilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031777">
        <property type="entry name" value="Sortilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006581">
        <property type="entry name" value="VPS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF27">
        <property type="entry name" value="SORTILIN-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15901">
        <property type="entry name" value="Sortilin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00602">
        <property type="entry name" value="VPS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="710"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="5">
        <location>
            <begin position="36"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="754"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="756"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8056832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="812" mass="92953" checksum="5FDBD63281BBF4C7" modified="2022-10-12" version="1">MRHIILLLIMQCFASNIDVQISYIDAPLINIHWCSDTVLALTSKGSVYRSDDRGRQWTKMSEIFHRKALIQLEDSDERVGIVNNLVPSPVDKSLVLFTGTDQIAWISLDCGKTITAVNAGKQLREYQFHPLEKDWIIASAWKQCGSDELLAGTPCVSYKELLLSQDTGITWHQIATYVNQFTWGVKNKEMAKYTPKERILASFEPQGKGHQSISSWNMDYNLYYSDDFFQTRTLSVPAGSRFMLTESFLFVAKVTSQVTQEVSLYVSGTELGQYKYSIIDADSKLLEHSYSILDTSENQVFMVVNHLKPSSPLGVIYISDSTGTRYSRSLENVSRLENSAEFYRVQGLEGIYLANVYAEDQAKIYTNQVFESMEEGFYAQQNGFKDEDLKKYKQTRITFDKGGQWVPLKPPTVDADGKPINCNKCQLHLHLSQAFYQFAPIYTQTNSIGIIVATGSIGKYLSYRQDQVHTYLSRDGGLTWIEIKKGSYIYEISNHGGLIVMAKDQETTNQIVYSWNGGMEWTTFNILDQRAEIQNIITEPQNKGTRFIAYGAIQLESNGIRTEQGLLATIDLDELHQRNCIGQEKAGEIGSDYELWTPSGLVNPECLFGKKVTYMRKRREAACYNPEQLERIISVTPCQCSQEDYECDLGFVMNNQQCIPINGTLNIDPPADCDGYYTVSSGYRKIAGDICEGGVEHISMRFSCPNNKNWIYDWLVIGGILFGLYWVYNNQEKVKEYFARTEMREIPQSKQEMLIQQIQQRQPKQQQQQQQQQQYQPIDLEQNEQQDQKFDEDLIEKDDDIQIEDETAHELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVP1</accession>
    <name>A0A8S1KVP1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0290051</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVP1"/>
    <dbReference type="OMA" id="MHTHPSD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24141">
        <property type="entry name" value="2-5A-DEPENDENT RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24141:SF1">
        <property type="entry name" value="2-5A-DEPENDENT RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="451"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="484"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8059560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="587" mass="67985" checksum="9ED0744A4AAC09D7" modified="2022-10-12" version="1">MIKLGNLLAQEQAKLKSELKAVQKQRTTRNTTSSTFYPNISKTSTLLYKSQVSPSINTDFISQNCSPINSPKMFTHPSDPDPSFSFNKNIHQSYRNKLETVQLPPNKPPLRRGNKITNLRNLNNNSIAQSNPLKGDESKEKLLQQTHFLCAIMQQSHSATGKIVANFLNGQLEKNSYESIIQMEKSAQALQQSAKEMSKALFISDDEGEFENLQNKFIDQIRFSKYRKPNQEKNIVFKRNYGQKHEMMIDQVASQRQKTDENQDFDVFIPKSQMDQFFQIIQDNIDGRMKEQTEQLIKDEQKLINGMIANVKKFREDIREVKYTMNHVRKNKKALLPSQQQVSAYRTLKTLRTLKSQSSARIQSPKTQRLDEEAKADLRLRVMKALRNFMDKLKRFNITLNDVVNNQVFPSQAYERPNSVEFFRQVKADNLREIESMLRDCRYHVYDRDNQQKTPLHHAVSNNSIEAIKLLVENGADLDARDMMGRTPLHIAAKNNNCDTVRVLLVYQANPSIKTVAGKTAQDLTEMPVIKALVKNAKKLHFMMNLQPSKKKKDFWVEKAIVYFQEDDPEKILKLQLYKNQAKLFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0Q3</accession>
    <name>A0A8S1L0Q3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0310132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L0Q3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="150" mass="17112" checksum="383019A21F3A7AB0" modified="2022-10-12" version="1">MGCALSNRKKSGQSQTIIFQQTGQNQGQQQLEGANIYLMGLEIEATNKHFSAPVKDSLLQIKRMKQQRKKIYKCIQSKSKHSSQMYKSESLEQSFSKKSEYSSKKQSSENSNQQQSGESNSENQLNNNSTYRNISVHEEQKNNPIIDEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4L7</accession>
    <name>A0A8S1L4L7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0300173</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4L7"/>
    <dbReference type="OMA" id="LIKWIPI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="99"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8060513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="414" mass="48869" checksum="33D2B6598F18B2D9" modified="2022-10-12" version="1">MYPLQKLKQISTNIDESQNMIEQENKTIQNIIQCHCCKQIVDSKYSKLNAKYICFQCLDQISLLKVRYDLQSQSKLFKSPASKYYCKLTMTPTSQFDFTLSQCQICYQNKILIKVCDNNHFFCETCLQTFIQTNFQNTVNCIQFNCKSNINYQLMYLYLEPDQLIKWIPITQQIHCIGQYCSGIWSVWPTYIQGNVKITSLNQCKCQLCHIQFCFKCRTVHTGLTCNNDILFENYKKQQKCFRCYCCNSLCQPQFCHKNDEIKKFKEWKPNIYECYICKKQFCFDCQSQTNITLFSNQQCDNCLNTFNIQRKKQKKIPIIKKIFQFLFTALILVLFSIYSMIFIQIPQIIDCFVNKIDPICQSQNPVKIILLIINIPFIIVILVLIYVITLIPLAIYNSFQTFIVKKGIINNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5X9</accession>
    <name>A0A8S1L5X9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">STOP protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0340031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8062867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5X9"/>
    <dbReference type="OMA" id="RANNWEP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="41833" checksum="7B427D6AC67AEFF2" modified="2022-10-12" version="1">MQGLLQPSQQTLLVRSPTEGSLKSYSRYESKGKKRIVYKNNKGEIVDPHEKLAKSQHSHQSQPVHSVKSRTATPTPSDSVKTQTNSIFDLVPIDDPIWLDLIPIEIQQDQNFNLERLIMDNQEYFVNLLGLYMQERQQNRIQEIKVSLPQKKQTTYDQAHKKLNALDQSNFNIKMYDQVPVAQSLQLQTQYETSYRTPKINPLDNYKPSYTKDNPEMLTLTSYKQNYVNWKPCLTERVGPQRGQSTGALPFIGKTSYQENYRVNNCEPMESAKKKSLGPFASGSSMIFKEALSKIHYPSYQLEELPPRKKNSNHQQGNGSYDGQFKTTFKNSFAYKVPEPIVQDRYWKQKLIQKILEKKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6C3</accession>
    <name>A0A8S1L6C3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0330063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6C3"/>
    <dbReference type="OMA" id="MQINVRQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="244" mass="28914" checksum="50334F8992B31E41" modified="2022-10-12" version="1">MQINVRQSSKSRILLAMIENYQKSSDEEAMLEASKNSSVAQIEKKFKKSQVNQKYQFPRTDENNQRISSESPIAKQFSPKEHNQQKDSFKDLLKKIYLSNLKKKSRKTQLNPEFIKQQYSILQQSHRKSFQTTNKSKLQYSLSQNRLLTDVGYKQSYSRGQSPQIKQFYSNRQVGTQLSKFMDYNAIEKQKSQQNFYQQHSCRSFELRYQFNKSPYSLLQNKSFDLIKQLSQFSSLQKFKSKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L8C2</accession>
    <name>A0A8S1L8C2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L8C2"/>
    <dbReference type="OMA" id="WISKHTV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="307" mass="35545" checksum="427AD9D34A0DEE4F" modified="2022-10-12" version="1">MQRAQSAAPNIDFPMEDMFKIATRQTSGFGIEGYEAPKKYVDPIKQMEERKFLTQKKGQKNRNHVTKRGHYLEDLQKLYEKLPAPNKYNIVKPWVPLKQEGRVKSAPQKRSTFIDQIFKEAKIRGIPGAGKYNVIPTLEDVLKKVEEDKKKKIAPVERPTYLNEIQHLAVINPGPGNYNPHRIEKKLKINETKPSDQIAKHKEQERKRGKSCLPDIGTYNPEPVEFTSFNKLQQLTKKKDKSDKNNFGREDRFKDPKKSKSKQVPVPGPGQYPMIVLWAGKEKDKNNPKKKNYLDITTRGISRSIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LA57</accession>
    <name>A0A8S1LA57_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0370013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8064980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LA57"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8064980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="18091" checksum="05E62FCAB132EF46" modified="2022-10-12" version="1">MNWLEQFFNTKILIGLGVGVLTASLSLIPTLKEQNKDNSQNQKMKLSTKSLPSIQDLKKVYIKKNEKQYLNRDSTSNDRKKNNKNSLKTETNQQDFYFNFDDVIRKKRKLQESDKLSSHKNHLFSKKFNDNTGLQNMIQQNNSKYQILDSKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBW0</accession>
    <name>A0A8S1LBW0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0340064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8062933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LBW0"/>
    <dbReference type="OMA" id="RMNESTY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8062933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="90" mass="10975" checksum="D7AC1CC3DA3F463C" modified="2022-10-12" version="1">MEQSLEKELLQRMKQSKYLLTILNHRSIEKIQNGFIGFQITQGLVYKLRVFLLKREGENKIFKILFQEWFGREQTAKDKMHELLQQYKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LEM8</accession>
    <name>A0A8S1LEM8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0390149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LEM8"/>
    <dbReference type="OMA" id="DCNSNQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="318"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8066558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="424" mass="51264" checksum="222C2C598B723AAE" modified="2022-10-12" version="1">MSQKDKKSVKSDKSKEKKKKSTKLDKKDSKILPPPTDNDQQQLIAKIISRIGDKESKSLANCDDDDDYPQIQKYNNNNNYQTNDFVIQNNRCENYPTVNKNNNQLRTNKFMIDQTIYQQQQQQDHYNQKNQQQYQDQYYSLNQTQTQYQNQQFTQQATFDYRYERIKDQVSQSLIPKRQEILEQVQKIDNRIDEIKYQSQKIENITREECEIIIDKLKSVETQKLQMLYHDKNELLRDIDQIEIFQQRISYLQDFNSQKNLHIFENIERLSKKRIKKQIDIYPDDLPQDLIQLKQLQLQNQVLQKLMDLKNEIIWKLFSDSQIENKKIKEELEKQANQEFQEWYNIVDQYQQELNRYKLQCTFCGINFDYQLINTDCNSNQQDAIIQFDCEQIPPEQFFGTQRHFFCKSIQNQQSQIQIENNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LMU8</accession>
    <name>A0A8S1LMU8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0410095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LMU8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="106" mass="12404" checksum="34497DED8F20DC45" modified="2022-10-12" version="1">MNQISQNILVQKLVDYKLRKNKNKRKSSPQFSPPKMPISQRQTSFSNGHAYSIHQKLKSIQSEEKVFIPQAIPQSLICLIYQVKCSKQYNHMNNRQVIYGPKILRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LNX0</accession>
    <name>A0A8S1LNX0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0420195</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LNX0"/>
    <dbReference type="OMA" id="LQINIKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641:SF19">
        <property type="entry name" value="NEPHROCYSTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_6G03870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="311"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8068509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="48272" checksum="1BCFE3C763899D76" modified="2022-10-12" version="1">MEEQLKKIDILIKKQNFQKSIKKLEELQNQLDQRIINPEEMIEDDYIIQSNIYERFATIFIDINQVYRAMQYLIKMIKIEKDICKASNNTDSILRLCNSYAKIGRCCFYCCYYEQTFKYLDYAQQLLIQNNLTNTGVYALTLTQLGNYYRFMFQDDLAEQMLYESIKIREELFSRDSIEVADSLHGLAQLFSDEGKIQDAMFSINQAISIWTNVLGYEHIKTAKSIYLKGNLYLRMKSTQENLEKAEQLIIQSLQINIKIMGNTSQDIADCYHSLGKIKAYNKCSNEFEEYFQKSQEILQELYGQSHASIAIILNNFGRSYYERKQYEEAVNCFQESIKIYTQLCGEMHGNLAITLKNCADCHKELGRFKQAYNDYSKSLEIYKKMQINQQQINTIQNQMIQISENFLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LP00</accession>
    <name>A0A8S1LP00_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0400113</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LP00"/>
    <dbReference type="OMA" id="MIPNIIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000003">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="5">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="5">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8067103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="216" mass="24899" checksum="3E4EAB110D91CB2A" modified="2022-10-12" version="1">MSYPFGNQFSAGMPNAKYKTSLCRHYQATRQCAIGAKCQFAHGQEEMRSINDPIPSALIAVLTVPPPYQEIMKPMMPQQPAFKIPCKYHAQNYCKNGQNCQYSHDVDIQQPMIPNIINPYQPQYPQVDMRQEIKQEDPMTTVFIIILQTLEQIFSKDDIIQKLKIAQDQVKCGNLTVGSECIKLIMTDPERTDDEIEQYTTIYNNACYYYHQLQQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LRZ0</accession>
    <name>A0A8S1LRZ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0450220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LRZ0"/>
    <dbReference type="OMA" id="NDSFHCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="52"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="482" mass="56799" checksum="9C4503521284E9FA" modified="2022-10-12" version="1">MGTCSSKKGTLKCINLRNLQNNLVSEGPASPTLIFSKRNDHTLDFNYVDEVIQPSNLINENYLKYYNQIRHVDKVIQNQNSIIVQHILTRQIFIAEVLDNNDKTNKYISLISNLFHENLQDFVEIYYYCSQYTIIKNYCNGGSLESFILNESKHITIYQKQIILGQIFNVISYLHSKSIVHGNLTLKSFEIVDQSQTIQIKLVDFFSVVISDKKPAIKLKFSSPQLVQAYLDNQIIEPTFKDDVWSLGIIGVRIVYNFSLFTGNSFKDFLENVLQGKRNQIPQINDTYYYEIQDLLNQMLQVNPQKRIDMIEILESSFMNSFQFNLQPRNINPIMQSLQQILFYMMAEMYSDNSLEYLSQLKNTKRITRFQLATLVRKQYKHIYDQSESLFAVNQIFKSQSELQYIELISRFTDKRSIITKSNLQKAFLHFSNQQHYITLNDILPFFQEKELQLKQAFKLFSQEKIDILQFFTLMFDYLQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTA5</accession>
    <name>A0A8S1LTA5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0410271</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTA5"/>
    <dbReference type="OMA" id="QTHFNTQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="278"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="122"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8067996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="440" mass="53091" checksum="F5336F4F4A477A54" modified="2022-10-12" version="1">MKSISQQTRSLIQKQQELGLIDSRISQLKQQLQLQESNNHIIEFKVNKILETKKNAFKDQSDSSRRIENLNQEQENRNNKIKLLKQLQEEDALRRKRDLDEEKRLEQEVMKERQMLYQLQYKHSQNEELQLKQQQFQKIQEQKFRMKQKLDMQRIEKIENVRNSQIKKKAELSAKFDQQQQEYQLKIEEEQQLIKKLSFSQFRYQALKDDLDLAQKISIRDYTNSTTNQGFIKQPFDQSKIKNVILTSPYAQIPSILNQKNIEKLNLLQKLLKPSFKDQQQTTERLNKKNNQTHFNTQLSDRTHKKENSSKFETETSKTKQNKFQPIKKQNTNLNTQRKSSHNLIVDQDRKQNQQSDNKSNSQDKQKMEEDNKENQQKDDYEQENSNNYQDDNDYENDNDNQTPQQDQDKQQNENQEQNQYNEDEFQDEEQYPDDNDEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LVN2</accession>
    <name>A0A8S1LVN2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0480204</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LVN2"/>
    <dbReference type="OMA" id="YINQEME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="256" mass="31272" checksum="61CCE184F17D5733" modified="2022-10-12" version="1">MHSNNQYKCNLKNEFNEYYSGQSNHTLPRFQLMKELCKDGQNMHEFIPFIRERDQMNRLVRINSEKHKLYTNTSRSPSNRNHTPDQFWQYNKNHIIPERRKKMSFKFNYQIKIDHKTEQILQPLITQQDNVEISQSKTERNLIYPPIETKKKRNNKFTNANVNVRQNEEPKKQQIKIPLEILQPYINQEMEQMKQDLFFKVDQDYQFPNDLTEKLMQQKKHIENLRQRLREWGEMKTEEDIEDLKQHAKFILSKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXN3</accession>
    <name>A0A8S1LXN3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0450150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXN3"/>
    <dbReference type="OMA" id="MIQINRS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="425" mass="49600" checksum="1F332F14900581CC" modified="2022-10-12" version="1">MKEQGRYSKLSEEEAAAQFELRKQQIMGNLLEQYQSSHKNKKTSKISKPQEINQQEFIVFNPKKPLQKNPKQKQNQLKLVKEESPQQNSTPTPEVEVEQIEQIVEPTPIEQHPSIVKIDSANLKKLLLKDIGNIDLEIKRLTRETKDFEMDKLANKSQFSYQSQSTKEVAPRNNPRQTSSQQKQQSKVYYELRQRPSSKESRISDSSYTKKAECFKGVLRSSSLKKLPQQQQQQQQQQQQQQQQQQQQQQQQQQQEQKQNQNLTQQKQVNEKQSMNKTVTIESENKEPTTNKISQSERKAPRPMIQINRSQSKQDSPYVDKKSTPPVKAMLKVQFNSIPIGSNSTKNNSASYFHHHKESYNSQTSFQKNKCVEAQIEEAFQLYNNLKFLMQSKSSVLQVRKDGNQMPRKTVDFFQHKEIAKPTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYI9</accession>
    <name>A0A8S1LYI9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0510268</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYI9"/>
    <dbReference type="OMA" id="WIPFAND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="6">
        <location>
            <begin position="8"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="7">
        <location>
            <begin position="22"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8073838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="39085" checksum="6023BB6B877A3DE6" modified="2022-10-12" version="1">MNNQDQTIKILKISTCSLSTLGSLFIIVVYFKMGMRRNFALKLIMCLTISDIIFSLSNLMYIYPGPKPQYKILCIIQGFLIQYSSIACFLFCFLLCLCIFWTVIKNRVNLSEHQMTFQLIGFCSPILFALIPIITNSYNTQIYVCGIANDDNDLYKNNQALFVQIESISLFYLPLWFFNIVGMTFIIRVSLFMTQLRSQSYQNQRSRNSDQQEMQLFTQQEYELQLSQRITQNMLLYPIIMLICYSGMTIYAIAKLCDQEIQILRGIRFIMADCLGLCNSIAYSYNAIMILRLKKMMQQQQALPMMKFRSSSKDSDYSRKQFESFLSIATIYNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYP2</accession>
    <name>A0A8S1LYP2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0490059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYP2"/>
    <dbReference type="OMA" id="IITHRQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="619" mass="72727" checksum="6204FEB293A17DDD" modified="2022-10-12" version="1">MMMKKSQGFFKNLDIFGQNIQLNFNGENQYQTAVGGLFSIAIIAVIGFFFQQNIVNFLNRNEINLNTQTIFDSNPDRIELNDNNYMFAIAIEQPQFNTFPFFNITLKQRIYTRSSNGTIYKNDTFIDLIPCTQDRFQNIFKDQNFTPQFTSLKLQEWLCPQYNYSIILSGGYTSDLFEFVKIAVSDCSNDTASNTILSWKPKCSSSQNRDLYLQQERSFRIKMYMTNNVINPLQVQNMSQVFLDDESFFSFLIQTGTETDVFYQKYNITTDDNVFPYLKEIQENVYNVKKAGDFKTSTVQNNGQQYSAIYMRRSPYTFVIKRDFQDIADLLSYLGGFANIVALIFGILIKSYNKSRFMIDLANQIYDFPIKNDTPEENQKRKEIKKTIARAKSRTIIIKPQDQDQQSQLQSPKYTQNQQLILNDTIKKEEQFISKQDEIYPAKSEFIITHRQEEQRLFQQEQEQIIKQLGINDRKGYLTQQIQKILNRSKPILFNYKYILSKIFCTKFFYDRNCILLQKAIQKINKDLDLCVIIDKVKEINLLKELLLTQDQLILFDFAPKQTINLLEEQQNMTRSLVRRTLDSLRSNSTQVESFNQDDSLNAYYKLFQVTITIYNIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYP6</accession>
    <name>A0A8S1LYP6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0470168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYP6"/>
    <dbReference type="OMA" id="DNVNKVQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="308" mass="34399" checksum="119F4ABB271D8196" modified="2022-10-12" version="1">MLRFIYQGFSTLVKQNPLKNGFRFENQNLTWTFGEFDTHSSAFAYGLIEQGWKQGDKLMLLLGRSNTSEAATTFAGAAKAGVITVPFRSNDPNEIEKTISIVNPKGIVFSPNQLIGETKFIEILKNLVPETTQTQSGESLKSSKFTNLKWLIHTGFYSYPGTYKFRESLVYASRNFNRLSLPSVTGPVTAQLRNGQLQTYTYQDLAKMSEKVQGSKHVIISGDPQTVTNFSIVVGGLERGYNTVLTGADNVNKVQKILKYYDNYSLVVDDSVLSEQQQSVDVNNLQNLFTVGDVNKAQNVFQKQAIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M2T8</accession>
    <name>A0A8S1M2T8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0520137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M2T8"/>
    <dbReference type="OMA" id="CLYNDLQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="125" mass="15154" checksum="3263FD98EE6968D5" modified="2022-10-12" version="1">MSKLKLIETIKQQEKLIQKLQSQIKRSQSHFQSPQNIKQQNTQRSSSRSIQNAKKLKEKDQTINILTERLNKAFENENYIKYKIQKQRVMFKCLYNDLQNEVYKLKTEMLQLKTPQRQSKKLLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3A7</accession>
    <name>A0A8S1M3A7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAYSvFN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0520267</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3A7"/>
    <dbReference type="OMA" id="ISIHYSM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039159">
        <property type="entry name" value="SAYSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019387">
        <property type="entry name" value="SAYSvFN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004027">
        <property type="entry name" value="SEC_C_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13527">
        <property type="entry name" value="SAYSVFN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13527:SF0">
        <property type="entry name" value="SAYSVFN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10260">
        <property type="entry name" value="SAYSvFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02810">
        <property type="entry name" value="SEC-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="SAYSvFN" evidence="2">
        <location>
            <begin position="147"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="292" mass="34777" checksum="191B72ECAB788249" modified="2022-10-12" version="1">MLKLGIKIGIRINVKSCDNTLLKLFRDFLRQRDQRIFIQKLSDFANSQIEKQKDNQSMDPYMKKIEQNNQHKDLIKDICSKFLKSKVKQNQRILLVEYSNLAIFDYYYYMYKVELVDSSEFKDRSKYYKHLLQTYFQWSFLKYFVYTIIWLIGGRISILYSMQGLYVIITGFALIFTNLGTREKGTLSAYSIFNKGYKKLMGQMTEEDVANMYNMGGVKKKKDDLSEDDDDDKISKDFEGLSMEQMMAKLSKLGNKQCFCNSGRKYKKCHYYIHQRIKQQEDEDRRKGIKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4X9</accession>
    <name>A0A8S1M4X9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0500131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4X9"/>
    <dbReference type="OMA" id="WDIGYLK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="704" mass="82385" checksum="E5198F81F39D7C50" modified="2022-10-12" version="1">MRNRILSKDDDNVKEYFSPQKRKQDNTVQIWQGYMSIALNSTEAGVRQFLSQMRRNGSFQQIEKKQKPVITEQEQYLETPNHSIVKSLDPQFPPFIRKSKDITSYEFLSQIYELHNRKARNQSLIEQLIGKTNNEIIVFPKTIHRKNIQKRSQRCQSVAQKDIELDSIKGQLIQVKQVQVQPLPKETTPKPLKRLIPIIKLERTTSPIKKAIAPQSQNKKKVLIDDTRYWDIGYLKFIQCPSVLEINQIINFTNALQVLPAQHQKYYKLYVGRGNNHMMVKSIFSMRAQWTVGGNLEEDDLNFIWTQKFVDFPQSEIKSIQRSISNETIEGWIDSNDQTLIRQAWDRVEGKSKKKLADYNLESQPILNNLCNVKELLTINHQNQSIRIHNHLKEGNQLGDKKFLFLNLSKHCQENEIDLWTLIPLTYHIQGPNDQSFYLFKKKFEEVSVDSQFKNIWIIKPGEDSNRGQGIKVCNNINEIYQHISQQNHTFILQKYIENPFLYQKRKFDIRGYCLITIINGAKKVFWYKKGYLRTSSSLFSLDSLDNQKIHLTNDAIQNKMNGYGKFEKGNKVSYEQFQNYLLEQNKQNNTNYSFEELYKNMKQLTKIACASSINQINNTDQVLGFELYGLDFMITNEFKPILIEFNTNPCIETGCPVLAKIISGLLDNLFRFIIDPLFPAKKTNLDDFNSKNDFELLLQSQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M7F4</accession>
    <name>A0A8S1M7F4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0550202</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8076037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M7F4"/>
    <dbReference type="OMA" id="HIYTTAT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="361"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8076037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="394" mass="46014" checksum="235036BF8A3AF22F" modified="2022-10-12" version="1">MTHLIKDTFYKEKLYQVSLSSFSFAQEQAGKQKEAKQPISEDVLKKALTTYGKTAEGQHYAYLSFMANNLDLTSLHGIEKFKHLQHIDVSNNSIKSLKPLNGLKYIITLKASNNRLTKLLDLKHIPLQIMDVDCSNNEIEIIPDLSCHRFLRYLNLSYNKIRQIEGVQKNKYLQVLKLANNHIDHIENLDGMNLTELDLFGNEITILDGLTQLPKLRKLELSQNQIKSLNGIVDLISVRELRMANNKISRIKELSYLENLVFLSVLDLCYNPIQNRRYYRWQVLYKLPGLRNLDGVQVPPEDIVKAENLYGMDLEDRKRIFKEVLPDEEFIDRRIHISELIEPETEDENENQEFIDQYDKTGKMMQKQTKSMGSQKSISEMYHSNKSLQEREYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M839</accession>
    <name>A0A8S1M839_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GPR180/TMEM145 transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0530247</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M839"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019336">
        <property type="entry name" value="GPR180/TMEM145_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10192">
        <property type="entry name" value="GPR180-TMEM145_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="GPR180/TMEM145 transmembrane" evidence="2">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="68" mass="7723" checksum="5E884B93A2BBDC2C" modified="2022-10-12" version="1">MILICQSIIMIIIKYFDGSLDKYHNFYGIGGWLLMLSKIGLTFLNSIGIYNLSKQVKKKQSIVLIAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8D3</accession>
    <name>A0A8S1M8D3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0520084</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8D3"/>
    <dbReference type="OMA" id="YRWEVLG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="40"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="158" mass="18936" checksum="87391CA172CC685A" modified="2022-10-12" version="1">MHCHLLEDPQINNNDQPKKIKKKNKYDYHRKKRRSSDASNTKKLGQKFTSEEDKLILQLVLNVGPKFQKIHKHFPGKTLAMVKNRYYKYLRYRWEVLGQNYKHLSVQQDSYENLCEQQKIISDQLDEEKSNLITQIISHTKLLSNARMFVEYLVEQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9R2</accession>
    <name>A0A8S1M9R2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1279 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0520271</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9R2"/>
    <dbReference type="OMA" id="TTAWICR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045866">
        <property type="entry name" value="FAM210A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009688">
        <property type="entry name" value="FAM210A/B-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377:SF0">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06916">
        <property type="entry name" value="FAM210A-B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1279" evidence="2">
        <location>
            <begin position="3"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="97" mass="10821" checksum="4AFCD69F44D57799" modified="2022-10-12" version="1">MQQKAKQFFATYGKLGIGVYFSISLVNYGIVYFALKQGVDMKQVGSKLGFDTAQGKWEQIETYGTPTAAYIIYKLMAPIKWPLVIGTTALICKKGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9U4</accession>
    <name>A0A8S1M9U4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0530133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9U4"/>
    <dbReference type="OMA" id="KFATICQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="660" mass="75944" checksum="2342EACA5D7E9982" modified="2022-10-12" version="1">MNFNLICPQHKQQIIQACIDLDCSVFPFMCKVCMTDQKIISLHTKHNQCLIPYQQYTTLVSKQIEDQLQDANKYIDLFNKESQVVQQILLSDEHLNNMEGYVRSQKQQIESDINQALESITLLFELHKTELINQLDQYLKIYKNNFFILKEQLEPIHFLMTKCKYYANENNLKQKLHTKPDLGSQLKLSIKQMSIIKKPDILKQILDKVKKAQELQYNNENFTKLLTDAMNSIKDFYIKNVSIPNQQPIEIQQQPNILTTIQTISSPPIKSKSMDVSNADKQTMLDDNKTVISAVDSKISVFNQLQPSNIPNINISVLDNRIIEDTNQSKNHSNQRFRIKKKLSIEFQVTSLALVSNAFVALGLADGTVKLLDMTTSKVSFYPNTYIQHTKPVSQLLILKQNKGTRLASLSDENYSIIWTLGENYVPYPERKLIGFKSKLNRIMDIADSSHLLCQSDTNLQIINYHDNRIAYTFDFKSQLIDFLYVSKYEKFATICQGNIVSIYSLKINNNLQGAMLLVNCELEYIQSALPISNISTCIAIEYLNDIIICYGCIDGSVKLFNVLKNIFIGEYQLFNSKVQELIIVKQNKLFILVAFAESIKQIKAIKIQENKINMIEMFGELLECPAKTGKNVIQTFFKNRSSFYLLSDSQKDIGLYELC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MA42</accession>
    <name>A0A8S1MA42_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MA42"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="88" mass="10538" checksum="63654F7175086776" modified="2022-10-12" version="1">MSFTYLPFHNLAYCPKQVIEFVQNPNLNLQDLNHMIEQLRMPKNHLKAIQKMQILASRQEQFLQDENTMQNQEEEIHSPKQLLSQIPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAB3</accession>
    <name>A0A8S1MAB3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA methyltransferase 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0570012</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAB3"/>
    <dbReference type="OMA" id="QVPPYEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18105">
        <property type="entry name" value="SpoU-like_MRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047182">
        <property type="entry name" value="MRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047261">
        <property type="entry name" value="MRM1_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00186">
        <property type="entry name" value="rRNA_methyl_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103:SF1">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="RNA 2-O ribose methyltransferase substrate binding" evidence="10">
        <location>
            <begin position="18"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8076699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="261" mass="29682" checksum="24E9D6269C0E5CE0" modified="2022-10-12" version="1">MYILKLGSRFSSQKSYDYLYGINPVMSALYANKRQFTQLYVNQTQQNDYINPRISNILNRAQSLKLDVQMIPKNKLERYCSNDHHQNVILKCSKLNYCNQLSDESNFVLLDSVQDPQNFGAILRVCFFLGINTVIVEKKGQCPLSPTVSKTSAGALELMNIFETDNLATFVKQRKHEFQVIGSGFESNSIPIEEFQKDQKKQKKIIIFGSESSGIRTELFKQCDTIVNIMSSRHTFPETLVDSLNVSVSAGIILQQVLNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MD60</accession>
    <name>A0A8S1MD60_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E2F/DP family winged-helix DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0600043</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E2F/DP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MD60"/>
    <dbReference type="OMA" id="PQPLEMM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000360">
        <property type="entry name" value="Transcription factor Dp-1, a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="2">
        <location>
            <begin position="103"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8078430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="302" mass="35231" checksum="7DD673C94A126228" modified="2022-10-12" version="1">MLQESQFVSISQKHLFLPNSPFEINNQSDKVYSQLPFEPQPLEMMFDNLPFQQTISQQIIKKPSKFSNLKKSTPPAILPQKRSSHNQLQLKMPNYESQKEPLKSQKGLKHLSIKVKQIVFEFKSTSYKDVAERLIQELIQEEGRISDCDNSKDEQNIKRRVYDALNVMIASKVLKKEGKKVKADFDTLVLGNNLLSQKSIQKEQLIDMQKIVEIKKKQLAEIVCKIKAANSLIQRNKSLELNQQQQLFYFPILIFTQDANNSKFIKDRKQLKILLKSKANFIADLDIVKKLYRYIKIKNNFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDJ6</accession>
    <name>A0A8S1MDJ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0540299</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDJ6"/>
    <dbReference type="OMA" id="RTCTFEE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007218">
        <property type="entry name" value="DNA_pol_delta_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14303">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14303:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04081">
        <property type="entry name" value="DNA_pol_delta_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="85" mass="10165" checksum="DD99FAA5DEFEC09D" modified="2022-10-12" version="1">MNDDYSRTCTFEELQSRIHKKQPSQRQISNLIDQFDWDSKYGPYSGMTREDRYNRAVVNGLQPPPEIKNYLSTKPHPYTQFYKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MET7</accession>
    <name>A0A8S1MET7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0570113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MET7"/>
    <dbReference type="OMA" id="VCTCCCW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="120" mass="14089" checksum="2CCA8076E2303D2B" modified="2022-10-12" version="1">MVEKSTMRWLCAVCTCCFWLNYYGFLNNCVRGCQICIDVFDATVSAVWKGIFCIFMCCCFGFECMTCVLCIYSCIELFNDPCQRYQAEDSWINAFWQLVYLKDLEIEGTVREKLKEIFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MGW8</accession>
    <name>A0A8S1MGW8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0600231</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MGW8"/>
    <dbReference type="OMA" id="EFNPNSM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000918">
        <property type="entry name" value="Kinase, Wnk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000427">
        <property type="entry name" value="Wnk protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="76"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8078749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="574" mass="66258" checksum="F02D98DBA4658577" modified="2022-10-12" version="1">MMRIENMKDDQIKIRNMILIKDLINSDLQIFEVIQLLLVMKSGEINQNMNDDTSLSSDDEEQTSKIIENDPTGRFSKYNEEIGKGAYKSVYRGYDNESGCEVAWNVFQLMNVSENEIRRARQEIAILKTLKHKNIISFIHSWQSKQKKEIVFITEIVNGGSLKNYLRRITRPKFKVIRYWCKQILEGLEYMHQQNIIHRDLKCENILIDTNNNELKIGDLGLSIQMQSNNTSSVLGTPEFMAPEIYHGNYDTKVDIYAFGMCILEIVTGMKPFCECKGGTGQVIKKVMESQKPQSLDVILNEKIKSIILECLKPANERPTATQLLHQYFQSTNNDEDNSPVQLNESLLNQISHDSKNSSFFKNNLSKNITELKGNTTNNINNNLTNQTNKLILFHEFNPNSMKQSEVRHCEQQNKLSMDTADDFSIEQQFGKQYKEYYITQNDETLLDLEQRQERELQLLLSLHKQQKADLSNKLQQTNRTIIPYGSGFLSSKSFSTLFEFNNDQIQQIQGNQQQSQILKVFPICDSTNSEENGRKTNNKEDDSNIIQSKQPCKFSTDVVMQSKVQNVRTPIQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLV2</accession>
    <name>A0A8S1MLV2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoglycerate mutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0650096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLV2"/>
    <dbReference type="OMA" id="RCVQTII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051710">
        <property type="entry name" value="Phosphatase_SH3-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469:SF27">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8081190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="25696" checksum="31619705922163FC" modified="2022-10-12" version="1">MLFLIRHGERADDSTNAEKQRIILSFDPHLSINGESQAKKTGKYIRNILKLQSIENIILVTSPYLRCVQTIIGIASQLEQQIQIYIAKGLGECFRQDWFEKDVLSELHYYKSSQFYDHVFKQYPLLDVPYQEVIQQYPESIQEFFGRFSENYKRMREYFQNKHILVVTHGYGVHAVNMLEKQVISNNADYCSVNVISYQKDNVDYLLLNSTAHLKKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSK1</accession>
    <name>A0A8S1MSK1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0680097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSK1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="34349" checksum="B052EAD8AC53CEEE" modified="2022-10-12" version="1">MYKSIISDHFQKIKEAIVAFIMEISGVEFIHFINMLNYTLQLMFESSFVNKIDIITIVTQKCIITIILKLLYIQLFDKSQFKYEKATQNCIQVLASIIGLFLLKTFFFGQVNITRWINVAYNPISTMRIEYKDYDKVLISLIVMLPLLLFTGNIQYQLICLFQPLSQVFIQIIKYLMNDEIEQFQLVLYTNLIGLPIYIMLSLFNGNVQNIFDMTYFFDILTMCISQMLVYLAFDELKNQYSSYLYNWTLFGCIIMLSMVFMYREDEVILIFLPLLVILIMFASISIQRQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSR0</accession>
    <name>A0A8S1MSR0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0630099</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSR0"/>
    <dbReference type="OMA" id="MVIVIDK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051731">
        <property type="term" value="F:polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032324">
        <property type="entry name" value="Clp1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF6">
        <property type="entry name" value="POLYRIBONUCLEOTIDE 5'-HYDROXYL-KINASE CLP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16573">
        <property type="entry name" value="CLP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clp1 N-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 P-loop" evidence="5">
        <location>
            <begin position="119"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8080113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="441" mass="51883" checksum="4347CEE4E290C7D7" modified="2022-10-12" version="1">MEIIELKEEEAYRISDPTDIVQVTLIEGAVEIFGQELLMKKLYEFPPHKPFSLFCWVPSKLKIKYKTKEDQPGYIFNEEELNYHKMMHDFLKINHVLESQRQMSFKLTDKIGPRLLVLGSHSCGKNTLCKTLINYSLVYGWKPIYVDIDPDNQQSEYPHSIRAEVQTCVKEQMQKNRVTYYFGYQYKDIVRDNSKRMLFDRLTEELSVQVDRKLQADLERAKEQKVQMLNQLGKVKDQKLCELIYMKSQEDKDEKEIYASGIFVNCPNFLGLHNQIIEQIKFLIEKLKITMVIVIDKPDIYRDLDKMQDIIVYKMEKLKGVVDSYNLKELESLNYTSFQSSQSIPENQLELYQMQVREDKQIMFEKTKSTNIVKLTIAVLHLKKTQLEESNSELNSKLILQAPLAFLAYVDNFDKDKGYLIQIPNDNCRQTLQNNVCIIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSY4</accession>
    <name>A0A8S1MSY4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0700075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSY4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="11350" checksum="001EBD08C990F999" modified="2022-10-12" version="1">MGKFTKDKRVQRNSSYSKQVFYNILIIYKLITIQSLFTIFRIFIIGKPKKINLELDQLISYYKQMILSKFLDKLREPQIYVQLLVVGHKFWLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MT15</accession>
    <name>A0A8S1MT15_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Mannosyl-oligosaccharide glucosidase</fullName>
            <ecNumber evidence="10 11">3.2.1.106</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0630263</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(4)-(alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + H2O = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:55988"/>
            <dbReference type="Rhea" id="RHEA-COMP:12806"/>
            <dbReference type="Rhea" id="RHEA-COMP:14355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:59082"/>
            <dbReference type="ChEBI" id="CHEBI:132537"/>
            <dbReference type="EC" id="3.2.1.106"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the glycosyl hydrolase 63 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.106" evidence="10 11"/>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MT15"/>
    <dbReference type="OMA" id="GMDDWPR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004573">
        <property type="term" value="F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000153">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.110:FF:000011">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031335">
        <property type="entry name" value="Glyco_hydro_63_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031631">
        <property type="entry name" value="Glyco_hydro_63N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004888">
        <property type="entry name" value="Glycoside_hydrolase_63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412:SF11">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03200">
        <property type="entry name" value="Glyco_hydro_63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16923">
        <property type="entry name" value="Glyco_hydro_63N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 63 N-terminal" evidence="13">
        <location>
            <begin position="5"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 C-terminal" evidence="12">
        <location>
            <begin position="265"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 C-terminal" evidence="12">
        <location>
            <begin position="545"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8080436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="687" mass="81474" checksum="322F53B01355B95F" modified="2022-10-12" version="1">MLYKWGTYKPQLIHAITERNMQTFNPLTITFYYFMNNNIRWEKSIKYKINERGNDEPTYFQYLYHDGERYAIHETIDGEYKVRFTNEFYKVNPEKIEDWVTTVVNNDALVGDEEYISLVYSIAIEKLNQEKTDLRKLQVEEEGDSLKITITTAQQVNYIYVTLESNGKQVNLNQNDNFGYSLQFQNPTLNYNLRTLLYSELRKTKEDIPFKFNKTIDQEQGNIVFVQYVAKRNELFKFQLRYMQREISVSSDYFFDARILKKLDNMNFFSQFNRTFNKIQLKNPFYLSCSMSSFVNLIGGFATSFGNIECEEANPICQQQKMMFTPTPSRFGFPRPFLWDDGFHNMIVCRWKSQLCIQSLLDWFDTMSSNGWIPREQARSPEQKTAVPQAFLAQKQSETNPPTFIFNFLYLDSLKNGEIQQQVRKLYTKAIDWYHFWIKSQGVNEGDNFLLFKWWGMNDNINFGSGMDDWPRSHNGLKSKFNIDASMWGWFFADSMQKLATIYDPQKSNQFHQTAEMIKKSINKYHFDPDDKIFKDVLIDGSFSPHLGYPNLFPIAFGFIDPTKTDILDSYVNMIKKELWTNHGLRSLSINDNHFGKGDNYWTGPVWIPINYLILRGIQKYYPTHKGLMDIYKELRENLMNTVCINWRDRGYFFEHYNQRQDGLGRGYHPFNGWTSTITLIIHEIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTL5</accession>
    <name>A0A8S1MTL5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0680131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTL5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="311"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="548" mass="65011" checksum="102EDA3DF1A0328B" modified="2022-10-12" version="1">MYNLVNKVIDAQTIQYPKRQFYIIKQLENTYEGGLYIANAQEWGDNSQQYALKIKKEMRDDEKALISFLIQEQNNKKRNDQNQLSNHIIQIYDYFECQQHHILVMEISQQNLLEYITQNTNISISEKERICYQITLPILWFHQKKYIHRDIKLENFIKVGNIFKLIDFGNVKQSNPSDKKTKQIGSLIYQAPEILQNSNMYTEKVDIWSLACVFYEVLSGLPLFDGQTYEQINTQILDKNYVLTKINQLNSSQQIKTLLIKMLVYEESQRLTIQYVYNELNQYLNNTPVNKMTIQDENICAYSTKFPNRKFKIIQQLGQGGEGKVYLCQSIDWGINDKNKFALKFLQNSNPDEISFIRNLITNENQYNQTQQMKNISGLIKIFDTFQWNKQDVIVMEVGQDLLKYLNQYQQLKMQYKLKILIQVQFFNQFFKLSQSIQQLHELKQIHKDIKPESFVIVGYEFKLINFGFVRNSFGIGNTISQEASPYQAPEIVQNSHGYTFSVDIWSLGCKLIIKQLHNKFNSLIKNFWRINQIRMIYLKIQRVFQYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MU80</accession>
    <name>A0A8S1MU80_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Kinetochore protein NDC80</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0710051</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDC80/HEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MU80"/>
    <dbReference type="OMA" id="TEMLCKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005550">
        <property type="entry name" value="Kinetochore_Ndc80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055260">
        <property type="entry name" value="Ndc80_CH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03801">
        <property type="entry name" value="Ndc80_HEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="1" id="KW-0137">Centromere</keyword>
    <keyword evidence="1" id="KW-0158">Chromosome</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="1" id="KW-0498">Mitosis</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Ndc80 CH" evidence="4">
        <location>
            <begin position="14"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="192"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="436"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="558"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8083898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="599" mass="71203" checksum="BB9A940F80D61C9D" modified="2022-10-12" version="1">MNNNRRRQSHSAERVNQQSSIKVQSEQKPPFLLDLQNEMEYIEDILILMARINRTQFQEPQQLKHALKTQNSVYLNILQLIMNVIDPYLEIKSFSDEKFQLLLKRLGYPCKHMSKTRFMSLGSSHTYLQSLHILSWLCRQAQQIIQINERIENGQYQPIEIENESFSNKIKKKLAEYLQDGYIGTIDDSALEKCYRDAKQKLTLELMQLQKDISKLTCELNVIKTQEPNLPEIEEQYKNLDAELMQVTQETQENQQILESTRQEINQNMNKYLEVDNEIIKVHKLIESKRQYIAQQPIKIDKAKEMEAKDYQLTIQSQQLLNELQKYQKEIDKARQEKTEMLCKISNIIQMLNSGKVKFDKDYETFEKNLYSYQQKLEQNNGIQIGEDENLLDMELDAQFEQKLANEQIDYKSRKSLVAYDLIQKQDTDSILKLLLPNKEQILQDLNLQLQDILNKLSISKRQALEMKNELNQKLKQLEDIECAKKMIIQEISQLNDSTEELKVLKQIYLEHQEKQQILLKEMEQKKLEIKFIVDRKNLDVIEIDRLKKQNQENQEVIFCMQQQAEIIEEKNTQLQQQFKDWVKDNLDQFLSLIQEVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MUD3</accession>
    <name>A0A8S1MUD3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MUD3"/>
    <dbReference type="OMA" id="QDSELWW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="252" mass="29286" checksum="D401F60D86F0B0FE" modified="2022-10-12" version="1">MGIQCSKRKAKPSEQFEIRTSQTQTSPQNQPTSKEALIYCDSSELATFMRTKPVKTFLADLMKKKQIQLDSCDQMAAIIESKSEIILRCYNDFTEKALKIKLKVLEYYNENYLRYKQQTHDQSTNQELLANETMQLLLETIIILNCLMDKNFDQDSELWWGDGYYDDRISVLSVSQDTSQQNDKIVQPIQQYLNALKNTIVQSIPIHEQQRVTEQPQSIANSTAIAQVHLTKFYHDLKLQFVRDVDEEKVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MVW7</accession>
    <name>A0A8S1MVW7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0720115</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MVW7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="123"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8084498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="488" mass="57139" checksum="C9AC7635155ED749" modified="2022-10-12" version="1">MRQKSQTLHEGGERKLQAVDYDKCIYNCLLTKKKFIGRGLRYLYLFKNLICIGKSPKIQIPERQFRINSELRIQWTYTKNKLKSVIFQVSGQQFEYHGTNDQLRELKQKCALYVFQIKIQDEYQAESVLGKGSYATVLELTNLYTNKQYAAKCIDQQRINEKKNGYKQLQQEIETMRILSERKHDNILQLHELYIGNQNYYLVMEMAKGGSLLSLMKKRQTLFSRQDIKLIMKQLLEGLEFIHNHNIMHRDLKPENILFMNKDLESLVIADFGLAQSVDTHPYTYPKCGTPGFVAPEILEQDTELARYTVSCDIFSAGVILYVLLIGEPLFEKKDRKEQLELNRKCEINLSKFPSDQLDDLEKDLLMKMLSKNPEFRWSANNLLKHKFFVSEDSIESEVDFYKLNNIAILKKQAMPTFSKNPFIQFGKNISLVRDKTQTISLRQRNCTLIMQTNLRFDKSSQEQLITEQVNGMGSFNLYKDEINQTNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N322</accession>
    <name>A0A8S1N322_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N322"/>
    <dbReference type="OMA" id="LICEYPL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="471"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="533"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="555"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="635"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="664"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="705"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="773" mass="89374" checksum="22C4EB589942A0EF" modified="2022-10-12" version="1">MFLFMLLSQTLACSDQNCVECNQTTICTACVSPLILSNNQCVSSCPTNYYPDSINNVRQCLKCNDKCLECFNGTPSGCTKCMKPLRLMCGNCIDSVADSGCSDCSSQCITCFGRTSSECYLCKLPNFHSPNTNSCELICEYPLYGDVNDFQCKNFCPSGTYGQPITRQCLPNCPDPYFKNQLKTNCVKECPIGTYPEDKSCLICHTSCETCSGGKSTDCIFCKEGLYMNNKQCGQCEKMQYSNNETRTCVDFITYSLNILEAYDSTIVFEISFSSSINPDTLDLKSQINVMDIDSSQYQMTLSSVQDDQFYIITLDFDISHRNTQIYCNFTNNIMNEINTPILFSSAIQDLQIPAQFIPPSQEANSNSINTYLIISYLSYFCFLFLLCLILRTQRRMFYLIINNLQFIQVMIFINFTFSTLTLNSLQLLDIVNLRRIPALGISYDGTILYFFNTPIYNQIPNNNFLYNAGYYYNFIANGGIQILGILIIFWVIWIISYILHNLYQINNKIETLKFYRTMLEGSSDLLVRVHEFLYFPLILISILQLWGYDFSSDINIASFVLSIVTILYYLLYFRSVYKSQFLDIDQQEMENKYFTFFSDFKQSNEIKKNYEFINYFLKTSNACSLIFLNSFPYIQFSISFTLISLYFILLIQRRPYLRNIMNYSAIFGYLIIIGLYTTTIISELEYQAKINNIAITNEDVEKQYNFGLSFMILIQIVLGFYAITFSYNKLQDYLKFLEKKPDWGKNFLQKMEKIMFSDKIQEVEMGNMSLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6B1</accession>
    <name>A0A8S1N6B1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0740080</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6B1"/>
    <dbReference type="OMA" id="PANCFEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="10"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8085326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="360" mass="42413" checksum="517097BC20EFFC08" modified="2022-10-12" version="1">MSKSIGKYVYDEKNLIGSGAFGKVFRGFDTEKKIDVAIKVSLTKNVRKEIAAIIQNEIQVMKRLQHPNLIQLYDVFEQDDNKVIVMELCKRDLKDLITKKSLQENEVLNIVLQLVSGLKELVRLGLLHRDIKPANCFECGGIFKLGDFGSVVEANYNEKKLMKQQFGTLAYEAPQMMMKEKYSVKCDVWSMGILVYESLFGQLPWQCQSEVQYLRAIKTWGVRFPIGKRISNECKDFILRCLVIQENNRMDWDQLFEHRWLNIKENDRIFNNSLVFEQIENQLLDHLNELTSQQHFHPNQSFQIYDFDNKGVLDYLKFERFLVQLDPRLTKKEIMAIFSHLDKDKKYVIDLSQFQQIFRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6Z4</accession>
    <name>A0A8S1N6Z4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0680163</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6Z4"/>
    <dbReference type="OMA" id="WGRPCIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002931">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045160">
        <property type="entry name" value="ATG16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19878">
        <property type="entry name" value="AUTOPHAGY PROTEIN 16-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19878:SF8">
        <property type="entry name" value="AUTOPHAGY-RELATED 16, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="503"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="66"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8082794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="535" mass="62434" checksum="00F17ACDC7DE06D6" modified="2022-10-12" version="1">MIDLIEQYRTEDIKRLSQLFNKPITQRLMEDYFKQNEKAIEFYQEQNRLYLKIEGMQECEVQPKELIELQMRNEELERQNRDLYTEVQETSKQLRHLKEQSELQQQCIKNLEVEKVESQNKIQKLEDILEQKNKDIREIVESRDKLIVEIEQLTIVRSQLEEMMLREKMKILEQLNEINELNESVQRQKRVLEQDQDRFNKQYKDIQTQGKFQTLAPLATEQQLAKNAFLDDIPQMVVRRLNKFHNVEIYAIAQNLNGTLVATCGGDRTIRLYDPFNLTQVSQYQTSSDEVFTSIEFSPSQDCILVSSTDNSCQLFYMQQWKQKMRGIGHVDQVTCSTFLGGDKDSCVSGSKDRQIKLWNINNAFCTRTLSTGSQVLSIISEHNVPVIVSGHRDGSIRGYSLKSDPKPIFQEKSFFDDSVTSLCISQDNHTLLCCSKEGYYIKSYDLRMNKLLKTYEHDKYMNSHDHNMISFGPDEKYIIAGNSDSSLISWNINGRFHKQLTKGQHEGVVLCVSFHPVSGQMYSADTRGNLVIWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9C3</accession>
    <name>A0A8S1N9C3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Group 1 truncated hemoglobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0790162</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9C3"/>
    <dbReference type="OMA" id="CPHTKYT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00454">
        <property type="entry name" value="TrHb1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044203">
        <property type="entry name" value="GlbO/GLB3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47366:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_37, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47366">
        <property type="entry name" value="TWO-ON-TWO HEMOGLOBIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8087829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="14172" checksum="A98575067166AF10" modified="2022-10-12" version="1">MDQNIFDSFGGDGQISDLIDQFYYKVLFDQTLRGKFLKADMTRVRLQQKTFFAQMFGCPHTKYTGKDLIEVHKNLNITNQQFDRFLHHLKCILSDMNKPAELIDQIIKKVDSHRKLIVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCQ9</accession>
    <name>A0A8S1NCQ9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0720123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCQ9"/>
    <dbReference type="OMA" id="HIINQDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="1">
        <location>
            <begin position="162"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1095" mass="128842" checksum="104821727E5CAAA3" modified="2022-10-12" version="1">MVDLINQIDFKREIANQNQSVLLPQNIQAIKVLITQSEIILEPNSKSLPIDTRLIINRKDFRISVEDLKSRKLQESSQLYSAFLGFINIKSVIFLLMVDRCSHHQLHPLYKDFFHIHTTKFIALDNRAASVTEIQEAVSNLKNLLSKGFYFTYEKCDDIDREEFMWNRGLCKQLYTQQIKNWDVLMIQGFMDSFSVYLEGKRVQVALIAKRSLKAPGTRLAQTGVQKDGSVANFVETTQIVVVANLKCQFKQIRGSVPVFWRESGNLLMKKLELYGNEQDNQIGFTNHFNKLVQKYERVLAVNLMNKNKQTEHDLIQAYELGIHQYQPDRLKYIYYNFDEITQGVDFHIINQDIMKISNFIKNMQFDAYDLITNQRKLKQRGIVRTNCLACLDRTNVYQQRVGLLMLEYQLKCMGLDLERILGQKILDINENNTKFLNPIIVLFKQMWGDHGDFISYHYTGTGSLHTNQERGQQSFLDILGSGMVPLSRFYRNNFSEHLRQESILTLLGNSTSERIYIDNDQSYLPFTTLEANDLSKIGYANLYYINYKLSEDQSKYTIYAMNEDQNIQIQCFIENGKQYNEIVKIHNELDSKFRSSIKIKNIDNKHCLLVELGTINLYQLVQNELHTFSELQLLQILLEMAQQIKLYQQQGLYLNGITAKSVWFQYINQNQYKIYFDTATYIPTTFNQYPTTIKPDYMDPIIIQKIDQQEQALERQENIYNTNDLITQHTNNFTDEQVQQAQIWILARIIYVILYYPQPTQTNNIDIMLPNLNQMIEKQKFANLAQLLKKMFRQDLISPIIDIDQLILETQKIKEQLGNQPNLQIPDKPFKQIQFQEKQLRCLVDLCKHFRCYEQGLQIIVQLQKLLQDAPLEDKVQLVSNHLFFLIYLGRIQEAFETMLEFQELLDQCSETEIPNIKQIIIETNVLFLRLFQEFQQLNFTNSQLKEILLEDPPLNLMHPLKSILYLIWSEISIEEDIQLNYALKAFEFYRNSKVQHEYLKIMIHIQIIKGYIKKQQIQKAQEHIQNALNIIIGDSLEKCKLYIELAKIELEQQEHKQAIELYDKAIKCGTKVYKNDKHPVLLQWQSMLAQIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEP8</accession>
    <name>A0A8S1NEP8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0870120</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEP8"/>
    <dbReference type="OMA" id="ICNIYVM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF0">
        <property type="entry name" value="UNC93-LIKE PROTEIN MFSD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="444"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="468"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="251"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8091080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="491" mass="57574" checksum="CF2122D456BCE92A" modified="2022-10-12" version="1">MNELQFLQYRYQLRIREQKSAKDLWLSTYCCFLYMLLKAFNITVKMAMMQIYDFIYSNNFPLDPDPGLVSFGNIQWFCYEMAAPFSLPMIPLLLQKVQFRYIFFFSSFGSILFILPLQFATICKDSDESICNIYVMWTFTIVFSFVSGFCKTLLLFSMLYYLSNLAGGREKIIYYSSFYFIHQFQWLIGSLIGNLLVDFCSRGSLLETLKHVYIILIISSLFLCSLFLTIPDEKKKSKFKKFNKMITQLLIQNGKENQKILRLELQKMNQKNESNQLYNKLIDLEEDNDERKKIQQIMDTQVQMMQTQQFFTIQSFSYEEHNLQDILSEKPKSSDDLHWVYASQKTISIEEYVKNNYFGNLYLTLSVLCLDGFYYFIILIFNVGSMLSFLLLQLPNLLTINSVQDPEVRAATVYEGFLYVGIGQIFGCFYMGLFGDFSQKITGLYYILGVYQLGCFLAWGIYIYQIDFLVFVMSFILGFSCSAMILSTQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NF17</accession>
    <name>A0A8S1NF17_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phosphatidylinositol 3-kinase</fullName>
            <ecNumber evidence="1">2.7.1.137</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0810184</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NF17"/>
    <dbReference type="OMA" id="LHKFAQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00896">
        <property type="entry name" value="PI3Kc_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057756">
        <property type="entry name" value="PI3-kinase_type3/VPS34_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008290">
        <property type="entry name" value="PI3K_Vps34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE CATALYTIC SUBUNIT TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000587">
        <property type="entry name" value="PI3K_Vps34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2 PI3K-type" evidence="8">
        <location>
            <begin position="18"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="6">
        <location>
            <begin position="566"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8088581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="884" mass="103750" checksum="62025A93CDF44733" modified="2022-10-12" version="1">MELQNYTNYTPHTWLQDVPVKLTISNLWDGNNVLDSLIDDPNKDEQLIIVASLYINGQLQYPTKTFKAFSRHSLQKQETIFFNSNIKDLSYNSLIALSIYSTQQKYEEAKPLGSTTFPLFDENLKLREGKINLLIWPNTQPDTSFNSKTPGLVKDRNIQDLNFCTRKIDEVSKGEQNDTNKNLSKQNLQIRLYYLSKIIPTAFLEVQLDQYFQQPILFEDYEYPQNDTEQQIIGGGYYKQPEDQIANQAKAPTQRIFIDQNQLSDTFRTSDFDQEFGKPDPIHDMMNLILRYSDHPETLKPQKDEEEKMRKMMARPLIGELKSDDKQLFVKYRYWAMQYGSALPFYLHSVAWQNQKEEQDALTIMKKWSPIDREDCLFLLSSFFCANEFKKSKKNKISMLEIRKYAVEQLSKLSDEKIENVLLQLVQALKYEPFDQKSDLVNLLFDRAIKSKSLATYLYWYLHVEKDGWHADSKNTILGFYYTTFQEFERMLQSKNEKLFKDIQGQSKFRQKLQSLATEIRSLKNDRQFRINYIKNQLSQSAYQNSSELPQHPCCLYPEYELIRAVPERTTIFSSAMAPIRLGFQVIKGDEESEIKLIFKNGDDLRQDQLVMQIFNLMDELLKGVSQDMKLLPYRILACSKSDGFLEFVPDNVTLQEIIIKKNQGMQPYLRQISEDKMNPYYWKTLSLKNNDQKDPENLKIIQDHIDQVVKRAQYGINPTFMTNYIYSSAGYCVLTYFLGIGDRHLENLLINNEGKMFHIDFGFILGKDPKPYPPPFKLCPEMVEAMGGQKSELYTLFKSKCVEVYVYLRRYAKLIINLFLLMADAGIKDYSQEAIEQMYEKFRLDDSDENAEIHFLGLLEESINSLFAKMTDKLHFWASYMRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFX9</accession>
    <name>A0A8S1NFX9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0740220</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFX9"/>
    <dbReference type="OMA" id="YAYCAMY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31652">
        <property type="entry name" value="LIMR FAMILY PROTEIN DDB_G0283707-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31652:SF0">
        <property type="entry name" value="LIMR FAMILY PROTEIN DDB_G0283707-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="465"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="240"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8085604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="507" mass="58088" checksum="5468B08B4BEF53C7" modified="2022-10-12" version="1">MDVFLIIISCIIAVLLLGINIYILALYCHPSDSGFGASLFCKILVVLGFTLAWGQILLVSADISASKGVFTTEGDSMMIVWYVIYCTILAMVAFLIPCAQFYYESDEDKPIMKRLLEVMCYEFILLGVLLTLLLVGFTYLGTARIPVHTITQTFNSQVSATYPIVLSNYAFNQIESDTNVEISVSFPVFLMGFLAFIGWFLLVLFGGVGLSALPIDLIQEYINRPKVIKSSEAMQKKMRLKNQAVELIQFGQQIKEEEKELALVNGFMAERKVKNQIKQKAKKFQAATEALQNEYEIFKLELQYQNVNPIVWIFKLILGIIFIILSLLWILQILLYMLIKINGFPAYSFLNKMFIYLEGTVMSFIGTILLALFTLYLLGCTYKGNLKFGLRIPFLFTIYPMKVNETFMNSFLFNANLLLITSVAVTQFSIQAFSEYTQNSQIALMFLGQIQYLYFFTWFFDYNVFVIALLSWTGIVLIYLLVRKPPKPYELLQIEKRKTQDFELQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NH32</accession>
    <name>A0A8S1NH32_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0750179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8086004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NH32"/>
    <dbReference type="OMA" id="MIVPTWG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052775">
        <property type="entry name" value="IUN_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190:SF1">
        <property type="entry name" value="SI:CH211-201H21.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190">
        <property type="entry name" value="SI:CH211-201H21.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="1">
        <location>
            <begin position="30"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="350" mass="39222" checksum="A8C9DE3178BCEB69" modified="2022-10-12" version="1">MAEQLKFTHPFPKLLFDDSHIQFKKYSKYIIDTDAGSDDAHAILIASYILKYIRTDAELIGITAVAGNAALENVIKNVYITTRIGHFGENPPKIYKGCRTDTLRRFYRDNYFLEDGLGGQQLRLLTELGLQDKPLEYFHEKQHACDFIKDSIYKYGEELCIICIGPMTNIYLTLQMYPDIVDKLGCLFAMGGTYMGVGNAANSVAEFNVQTDVEATAAVAMAKFKQKMLLPFDVVLAYTLDKQNGKVIFENGANTKKSQFVESIFKAVSHDGNYILCDELAVMVALVPSLIIYSLQKNISIVSDGQARGQVIIDWLTLMRGNQKSVVSILTAFEWNSVIEIATKANQDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NR43</accession>
    <name>A0A8S1NR43_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0910067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NR43"/>
    <dbReference type="OMA" id="TGTQYPE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="198" mass="23505" checksum="3A9F7BCCCF424E54" modified="2022-10-12" version="1">MNHSLYTVHPTYTPINLRLAEEYFNTLPSGDISRQFYDGVQYRAVKGLFASYALNRFSQYGKLNSLYKFYPYQKIVKETKEELCEDWTSNIQLAAIVTGASWTFLYQYAKRGQVLSILREYGSVLKTHRLFRQYLYTLVLPFAIANEYTFIHYHDHIEQLWQVHANRLNKKDLSDPAGTQYPEELRAPRRDVFINKWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NT72</accession>
    <name>A0A8S1NT72_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0980179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="327" mass="39644" checksum="AC10FE91E2A63F0B" modified="2022-10-12" version="1">MGGGQSKAQKLQSQQRANKPQLPQHQTPKQHNQFLISEPIQRYFWNSQFDYENLISEILFSISIKRCMEYLQQFKLHMRYIKKARNYIMMLHNVVYMNQNLIINIKNNDCVLIPFFSLYHISKFQVIFSSSDKKWWEGKYFVITQKKYKGSIQTFVLFMSEYFQSRFMNQNQKMIIKKLILYLQEYELIINYNSYIVTAYLIQQISINSLKNRNQLNNKIIKPIEDPNQKVMSKNRNNNNNYCINNSFKIPKFQHNKQMIWKNNLNSCLITLQLKFKNLAKNKNKXTQIWLNNQLKQQLSYCIYKHLITQVFWQIWINICHRFQILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTZ0</accession>
    <name>A0A8S1NTZ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0940038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTZ0"/>
    <dbReference type="OMA" id="CQKHCKN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8093751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="171" mass="19554" checksum="68EEB3C4C6ECDF51" modified="2022-10-12" version="1">MQYVPLTVHLSALAQLYQQCAALSEKHDRLAKGLDIFNTEVNHFTVAPIFDQERIMQEQNTEGSQIQDFSSQKPNTPLSSCKTPVEIDLVTDEDAQIEQGTKPIQNETKKIKKNKQNVNSQNFLQCPMCQKHCKNIAGLRRHQSRMHVDKPLKNVTKKMQNKKQLGNNVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUJ5</accession>
    <name>A0A8S1NUJ5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0940114</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NUJ5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="421"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="3">
        <location>
            <begin position="369"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="488"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8093901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1030" mass="122049" checksum="73E1A3CBA4B5D890" modified="2022-10-12" version="1">MFNIEFDQDVELRAVQNEDFEQQYLDRCLREYRKEPNLTIPQISRTQSQKTIEAECLHKISETPFDENVILTQAQNSAHFSNKYLQKHEKRCSIPNAGTSLMCSFYIRRFIEKISQSRKKLLSMNEIHFNLIEDKAADMQNLFNHQSECKRQGYTLGKMKTLLRKESVINDLSTQEQIKEQIRKIKKQILNLLTVIINKIPIIQPESRLKMIWDFFASFFRILLVILIPLEIAFQPCILFTDFIVLTAMILLILQIDFLIRINTLSYRNGAAIQNRWELLVYQFKKEFFTDFSTSFLLIIFIMIPEIDNNLNLFLLLTLAQYKYIHETFAKSDQISYLTRPLRGIIGLVKFILTLLFILHLFSCIWFWFSKISIEDSWIKFNGLDLKSWELQYLEALYFAVVTMLTIGYGDNVPKNSIEKIITIIFILGACLWFSYSVNFIGGIINDITQNQVERNRKMRVINKYMDQRKIPFNLKHKVKEYLTFRWKEDDEVDLEIEQTLLEQLSDELKEELDKEAHKIFIQKSALLQHFSDELKDALSKSIKRKIIPPQNTFSIDFDDHQHLCFVEQGVLLYQHLDRKQRSKMNAPIKQGQFFCVQDFIIQSHQKDYFKSNGYVSLLILSKLDFMTTIKNYPEDFQKFYEMRELMTLSLKPPQLENGVFCPACLCFNHSLAKCPQIQYIPDREAILKRYLLPKIQERKQYYRDFTKRFNSDQVISRSRLEKDIIQQFAQVFQSENHQLIQEQQKVQLIYEQESLSSCVDPSPCNKFQNDGGFQAAVLKSKRLISETSIQLSSNAKLQDTKSSLHHALQSRKQSIINLNIPSNIGKSPRNLLIPKVAIIKENEENEESSNCDSDDSNSNRNQEIKYQLSISDFNENMMENVINLYHQLQRELESNENDQLIQKAYYQIEPIYWSFHQQNLSEFEMMTTYDYFFKNYNYNEIIRLAQKNTFSWQSQFINKLQRYMLYPFLFIQKYMSKKRNLNNTEKIKRDPSKRLISRLNTLKNSLKLKKKQSIIIKPTQKFGQVAPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NY62</accession>
    <name>A0A8S1NY62_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1020022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NY62"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="122" mass="14444" checksum="CD823BBD159F5D47" modified="2022-10-12" version="1">MMINFQICSVETGKGHMLKFQTNQTHGPELLIYPERSQPLKIPARPSQYRKRKWVYSFLQVQNIKKPTSKKIVRQKRRNVSYVHYIQFSTIFPSLNNLCRQDCNSILSLLIQSNILLFVLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYU0</accession>
    <name>A0A8S1NYU0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0970096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYU0"/>
    <dbReference type="OMA" id="DPEEFYC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="402" mass="47105" checksum="4406FFA9B4616C39" modified="2022-10-12" version="1">MIIQKGNKIQIDNPNEELDKEVLQNCKTHEAFFRSYCTFCYPSKDPEEFYCQSDQEKPINYFILDFQENKYLDNQLYNAGISSYDQFLHKDQIANLKNKQGTDPKDIAIQLGLYTQQDVEELIDRAVQENWQDLQEFSVTVDFWEISQTKTFFNRAEQILEYLNLHNSIIIYPSKRAQFIKMLEINQKQASRFLASFQIIDQQASQLKSDTIQIQRKSSLPEYADGQQKRILNSLLKTTQYFPFGGEIVYFTDDYKDLQTIAFKFGKQSTPFNIYCDPKDIDKYYNVAGLSGGKVYSYFSEEKRQITHKLRAIISNNYIFTESQTNKFEKFYTDINFPIDLDYELKDPTNDNPGGCLQVILEDDDGYYVEQKIIQMNSNDDLHEPSVSLSQETLNLTDLPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5A9</accession>
    <name>A0A8S1P5A9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial 2-oxoglutarate/malate carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T1060099</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5A9"/>
    <dbReference type="OMA" id="MMDTSTM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000009">
        <property type="entry name" value="Mitochondrial 2-oxoglutarate/malate carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035771715" description="Mitochondrial 2-oxoglutarate/malate carrier protein" evidence="12">
        <location>
            <begin position="22"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="5"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="105"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="206"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8098199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="33985" checksum="2ED1A8C78CD33E96" modified="2022-10-12" version="1">MPTLFELISPFLIGGISGCTATTFVQPMDTVKVRIQVRSELKGQGQTINVNTLEIIKDIIKTEGPFGFYKGIGAALLRQVTYATTRLGLYRAIDDHYKRTHGRSMIFWERCLASSFSGFVGSIVGNPADLCLVRFQADTLLPEAQRRNYKNVFDALYRIVSEEGLITLWRGSLPTVIRAIAMNLSMLTTYDQIKDIIVSLHGKGKEDYMDKLLSSAAAGIGCAIASLPPDNLKTKLQRMKKDPTTGQFPYKNIGDCFLKTIKREGVTGLWVGLPVFYTRVGPHAMITLLVQDTLTQMWNPPKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6M1</accession>
    <name>A0A8S1P6M1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Katanin p80 subunit C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1070059</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6M1"/>
    <dbReference type="OMA" id="QNDMKQC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008352">
        <property type="term" value="C:katanin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028021">
        <property type="entry name" value="Katanin_C-terminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19845">
        <property type="entry name" value="KATANIN P80 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19845:SF0">
        <property type="entry name" value="KATANIN P80 WD40 REPEAT-CONTAINING SUBUNIT B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13925">
        <property type="entry name" value="Katanin_con80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="131"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="173"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Katanin p80 subunit C-terminal" evidence="5">
        <location>
            <begin position="378"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8098474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="550" mass="64693" checksum="362F5BDC31AE81D0" modified="2022-10-12" version="1">MKQNTIYDYQNESQIICCKFSLNQNQFISVNEKNIFLWSEQVYQPLMTLSNSAKVSCIQFSQQNNLILEGTQQGKILILNLEQQIQQEIKSSHKDVITNLTNYDINTYLSGSQDSVIMIHDLRQNGPIAQIKQHKQQINDIVTDLDTFRFICGSQDATISVWDFRNYSQYQALLEHQDSVNNLLMIKNTNTLFTSGDKFTIQSWDINKQTQKNRISLTQQPIQKMAFDYQQDILICTQQNNLDIFNTELLQLNTMQMDWQRIKDIKIEDDIVKILEDRGEILKLHKFQLELNGLQVEDNYTDIKDIEIPFSQQMQFDQCFYPSQSKENFDQSYEQEDQISDDIITTQKLSLKFSQLIQTKSNKDKYIQLEIIQEISNDHSKIKMILNDRIQKLKPIIKLYSQNDMKQCIQQINRCNDNSVLIDLFALLKLDPAQRKIQAEDIILLLENVIIILNSKYTYQVIKGLEFIKFCFYQIKDNILSYKTAKKQPQNKAQDKNQIRQKSYDTLILQFDKIMKLPKLNKLLQVKDTNLVNLTSQIKNEISQFLNKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8F6</accession>
    <name>A0A8S1P8F6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1090102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="297" mass="34819" checksum="10A39413015330C8" modified="2022-10-12" version="1">MEVFQTSNQKQIRTKQNKKQFNLFILGKAQQKKDEQQLKDTFQYYKLKDEYLQYQIHKNTTKGLMLFQNKNINKSFIDASSHVSIEQEQLRTRTPDIRVLKXSSHNPQREQMLKSLYQFKALLYANDPGIEKYLHDLQGQKSPHKRDNSQTKKSSSVRKEQEPIMRHPKRKTLGCGHEIDVVDSSGECLVCTTQSPFDEFKSKCVNVFKALQSLDNDDFFRILQQLDDPLTISLSKQRCQSSTGELFKDPMNYSRMALAEQQTNIQQIIRKCLRCNKDIQNHDSKNISYFCNSCKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8V0</accession>
    <name>A0A8S1P8V0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1090048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8V0"/>
    <dbReference type="OMA" id="HRTEKYR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8099241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="169" mass="19785" checksum="0EB7D5D4342D246E" modified="2022-10-12" version="1">MNCQEYIRYSDILDGKYEIDQLESIHRTEKYRSTSSVQTNHYKDLIQDLIQAQSRCADNINQLGYMLNQQSSKRHTQSDFSTPKLNMSQVSQISQIQTPNKPIQSTPASPQLLNQLNNDNLLDQIDKLIKIRKLDQSILLRIIRKHMNDCPYFVQLVKQEFRNSINSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PAQ2</accession>
    <name>A0A8S1PAQ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1110066</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8099974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PAQ2"/>
    <dbReference type="OMA" id="NLESDNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00839">
        <property type="entry name" value="MPP_PAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041792">
        <property type="entry name" value="MPP_PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025733">
        <property type="entry name" value="PAPs_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45867">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45867:SF10">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14008">
        <property type="entry name" value="Metallophos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035890800" description="Calcineurin-like phosphoesterase domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="449"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="3">
        <location>
            <begin position="133"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase C-terminal" evidence="4">
        <location>
            <begin position="369"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8099974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="58949" checksum="D7B1145F7AB2454B" modified="2022-10-12" version="1">MFMIIFLFLMESIQKQCEAYGVRLFLGHYYTSLKIDTDALRIVFNTKNICNNQFQLFIKGDLEFNITNYKTKLLNMTDVYIGTKANIEYETYIHTFQIPKQLSSIVFYTLISNDTIIKQAQVKLPHLQQETTKVLFFGDMDSRWVYNRSKQTFDWFENQIKQNIQFDSLIFTGDMAYNLESDNCQRGELWLSRISLFTSQYPFMITPGNHDSGYNRKQIFIREHFEMPYQNMHNTQDYENYFYSFNIGFVHFIQYDPVKIIYKADPYNLIRDQLLLQFRNDLITAVQNREEVPWIVVFTHYPIYCNFMDDDQCVNNFQYLAEFEKLFKEFHVDLYVSGHQHNYQRNYPYYQNNSASFEMDGNIYYNYESPITIIEGAGGADYGAEIMILENKPYTVKQMDQNGVGLLQVMNKTHLQFQQIRVSTNQIIDEFWIIQNRDTYDIDYFNLEYWNIFLIVWILIFLLILILFYLFRRLKLKFNKQYLHATDFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PAX8</accession>
    <name>A0A8S1PAX8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1120051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PAX8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8100327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="52" mass="6283" checksum="23CD136D8E700978" modified="2022-10-12" version="1">MYDFTNKQLKDMIYEEILLYNYPEFKKQYEEKIANNQSVVSHLIKGESAKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PD76</accession>
    <name>A0A8S1PD76_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1130061</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PD76"/>
    <dbReference type="OMA" id="YATFAIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000210">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="106"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="583"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="471"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="586"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8100713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="603" mass="70386" checksum="79C9428FA7DD0E01" modified="2022-10-12" version="1">MLDHKRNLSQKNIIVVVTCQHKQVSIEISIDRNIAELMNKLHQHPEIPNAQQIVALRTASHSITIDYLLSIPNERAQLSIDKIKQENLIAVYGKPQTKSTLTLRDFKFENCIGKGGTSEVYLVRHYISGRLFALKMIKKQYITDCRRLEQVLREKKILSQVMNQSKFVIPLYATFAIKDHLCFLMEYLPGGEMFYHLQHYHFTDEEARLYISEVICALEDLHQHNILYRDLKPENILIDIKGHIKLTDFGLSKLDLKDDQLANSFCGSPEYMPPEVVNRQGYSYPADFYTLGCLLYELLLGLPPHYSQNTEEIFYKIQNEEITFSDELNSDVLSLLQNLLEKDYNKRIHDFHTLKKNPWFTTIDWSAIRTQSIKKMPISFNLKDTNIHQEFLQMDVSHLNQQNDDGELNSCDDLFDFFNFVNDQLKDVFQFKPKLRVQSDHIIDLKLLQLRRQSNNNQNINSINNNNNNNNNKIINKKKNLKLNLQDIEKQMKFTKKQSLTPQNVMPLSLLRKSFQNLIKDKPQNRNQDKSPTYISILSDRPAFDKNKILNILSTLNFQKKGQSSLHQQSSLHQQASWRPMEIKRGSSSSAHIKSNLYSISKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQ77</accession>
    <name>A0A8S1PQ77_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1260088</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQ77"/>
    <dbReference type="OMA" id="KIITVAC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894:SF5">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE RSMI, CONSERVED SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="955"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1139"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="312"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="637"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1081"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1140"/>
            <end position="1150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8105161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1159" mass="136069" checksum="67F807BC1D2E5CE6" modified="2022-10-12" version="1">MHLIMKRLNISLIPRYIILKQLIIYSMKQQQLPSFTETGLKFKTLSHTNSLLKQISSQQISTRSNNTTVTQNNIHIAEQLKLAKAEQKSQQNSFLLNSVVQNSTKQLSNKTTFRNQLDLEEFVKFTKNKKQANLLLEKDRKEQISVMSRVREDVSHYYDPIKAERKKSDNLLDEKKRLERKKPLLNRRQISQMSSPQLSLLDMNEYLKFETSLNSEIRNLQTDAPMGRVQTIKLKEWFDKQKQDDQPDTLNLLSVTIKELIKQIKFECFERGLLIEEIWTKVVNMINDMKFQTDMFISKGKIQMLDEYATQSKIFSYEIESLKAEVKKLNEQLITENKKNHVLKDDYKELEDQFGKMRLQSNDLKKIVAFLTKKLKQANSEIELLTKKLQNEKKHRFSQVAIPHNEIIVNQSDKHQLSKQNSINNAPSMFYLKQGRNSIMPQKTDSLQNSLQSQVVKQESSSEEERDEVIEELMDDKNIIEMDNMVMNMDKIITVACFAQITGLDFLEYATKEQSCQTLISMQKSDFNYFTQSQEKFDQVFEQQKIKQNIEELVKVYEDKFKNQTSSRQLNTLIEKKFFVQKDANEMPSLTDKNMLDSEIFDESKRIIHTQVDIQQQKQHNIQQVDLEKVKTLIELLSGAEMQNKQKDQKLNEMQNSIKSLNEQIEEMKKKLSLFSEADLYNVACGKEQIKKEQKQIEQFLESQNLKSEQKITVRKRDAKKMTQAKLIFKGPQLGQKVTVVYEFQKQNAGPMLIEKIKQKQLPKIKHFMPLKLLIKQINVIYADRISQQKENSNIKEQDLASFVYSYFLSQFGIKKIAEQKFLILVVSVKHYKSIVRINNFAKFLNLFDTYVNFNLDELKRYLEAYDYITNVSQLGILNTDQDQELRYMVPYLRVVQYIGIFADSRMTSEEKEELKKELDLLKENDPKQVNKQHLIDFDQFMIQLFAKYKILVSRAKEYVINAFAACDLDGNGMCNFEEWFLLLRHIEPDRLSSEQISEIFFANADLLVKGEQNFSFEKFAVVCVEFGLFSEEAQNQFLQIKGQKTEIMSQFQKLLDSWPTIRGVIEQRFDQIVLLDADRLDQWRQIIDTLQKKIQELSSHLQQSGIEKKVKPLLIASLLLQKESAMLLELQNDYEDESMGSQLQSNQSIRLAEDQIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PV78</accession>
    <name>A0A8S1PV78_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1330043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PV78"/>
    <dbReference type="OMA" id="PLNCKRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="218" mass="25748" checksum="0BA1B1A3F67C9EC2" modified="2022-10-12" version="1">MPPFTPDARNLGGRNLISQQIWNHLQQLKEISQQIPQLLISPRKIPNSPKQSIEPLNCKRMNRKQDYLLCPTYLFRRKTVSESFTSLSPKHIQDETQLLFQEPLLRRQKNMKSPQKIFPFSTIQLGALYRVKANDIKINKNQDLIQWNEVEQEVKDLNNNYDELLIQIQQIIIDFGIFSAQDYQILIQMILRIKPQIPKMDLLNIFNELIIKQQLQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PWN1</accession>
    <name>A0A8S1PWN1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AraC effector-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1330005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PWN1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010499">
        <property type="entry name" value="AraC_E-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029441">
        <property type="entry name" value="Cass2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053182">
        <property type="entry name" value="YobU-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36444">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN YOBU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36444:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN YOBU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14526">
        <property type="entry name" value="Cass2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00871">
        <property type="entry name" value="AraC_E_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AraC effector-binding" evidence="1">
        <location>
            <begin position="8"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="173" mass="20031" checksum="EB2F6D89E5894C99" modified="2022-10-12" version="1">MDPQIQAPKRKIVEQPSFTIVGFQRNILQKEELDPNTGQIRKAHEDYFSQQIHDKIPHRLHPKRTYCIYYEYQNPNDQNENRYKMVLGEMVSEVNDLPEGLVAVTVPAHLFCCFDCGPGPIPKVVIDAWSSLSTLSPEELGGVRSYDFDFEVYPEDLNLKENIKFELFIGIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q251</accession>
    <name>A0A8S1Q251_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1410137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q251"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="195"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="469"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="304"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8109736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="508" mass="59967" checksum="EE90E716676B958C" modified="2022-10-12" version="1">MFIKAIHSSEQEVGRNVRSSKKKYLWKFSIDNVDQEIALFISSLSGKKEVRHNGRPIHQESSFFGDFRYLHYIQKCLVTIAPRFDSYELYLDNIPFAEYYNTRNINQSHNPQNRPLNNDDGDQYDLSPLKDPEPPKKISQDQLQKLKLTVPNEQTNITSNKTVTKQQELWWKQGVNEEFANFSTPSTNGTDNYFGNFGFTFDTPQKESKKSTQQNQQQNNHNQNEKPNPFFEFNQNSWNVQSQPQNNCNFGCTQQSSQIKPLQSPQQYSEPKLPSKTEQQYNNMITPQILNQQNQNQLFNQYNFSNQQQQSQQLIQQQQQQQQQQQQQQQQQQQSQQIQQQQQQYQQQQQQQFQQQQSQVFNFQTTQPQVRQQQQIDLLDLFESCQPQKNNTIQQQEFKKSPSQASIMPLQIQSQITPIQLPIQQNQILTNQFPNHLNYQSQSQYSNQTQSTYPQYRTIQSLTPLTSSYQQTQKQMPKELEDMFDQPAQVVQPPIQRRSTPFDDDLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q334</accession>
    <name>A0A8S1Q334_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1420140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000146">
        <property type="protein sequence ID" value="CAD8110049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q334"/>
    <dbReference type="OMA" id="DTVPKIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="336" mass="39187" checksum="BD54EDF27E1D1C4F" modified="2022-10-12" version="1">MIEPYNHDSDIFDDEVEHPVSVYSLKAHFKDDIRMRMQDKQLSELDVQDLNQKMNQLTLNKCNFKTDIRRHRKLAQKQQECLNIEKQTQNDSNQLQATLKQRQQAIQLSCTDQMTLMDTVPKINFIREMKDSINQDATLDLRIDLKTQSQSTFDIRKLRNGLSPSPINRLSAKNQSKLSQRSPEHESNLDLSFNYTMQVASQGSIQYQDNKFEQQQCKKSTINQKHNHQRIKLKTQQHVHSQRSSQLSLLQIDQEGFRGRSLSSVSTPKSILKNPSYQNSVLDIVNKIKFQGRPCFSSLNLIDSTPKKLQQNIYSSAKRVKFCLTKQQARRENIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDN5</accession>
    <name>A0A8S1QDN5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1540116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDN5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="213" mass="25986" checksum="2FEB2C6FB3FF0442" modified="2022-10-12" version="1">MLNLQVFPSLNYSQTQSYNGIETNNYLLRFQFISLIYNIFDISFSSNYNFRYLFTHFNFTQYIIYKKIRTLNIKLPKYDSRSLDRLYPCILLILYWLQQILKKYKKSKRYSELQELHIQCVVDELKGYNKEENFHKFFEVLQSSLSTIGSFKFLVLCHKLIYQLQQEFAIRFIQNKLIPGDDTDKSRLAIYYYNFLFKLCENFDYYKEVIENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QEZ5</accession>
    <name>A0A8S1QEZ5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1550014</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QEZ5"/>
    <dbReference type="OMA" id="SCGYRVC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044613">
        <property type="entry name" value="Nep1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468:SF1">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="224"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="11"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8113347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="427" mass="51062" checksum="3D3F8C0A434E537C" modified="2022-10-12" version="1">MNGFCCCFQSRKGSDKDRPEKNQTQDQNIKKSESSISKLKNQQDIQNKNLGIQNQQNSQERNSITSWNQDCINNGFDKLQIQSSNFNNLIIFINILCYYSEDSIKYYQNQPNQYECEVKLYQTEYDENNNPKFRLNEGKFLINLEKKTFNLIKSGICFQEQSNFINQDFGEDTIQKNRIQENKNQQIKILDPETNEQLNKSNRDNYEYICQQQQNIKIWCKYNQQFSINDLNILKNKLWMTSSIIDSYVLYLNLSSDNKYFQLKQTERKNKKRILFFPSSLTTNFGDDYDIKKVRYLFESEKPQFKDINFQLSLLYHYIGFPVNKKNRHWLFLLFELSSKKVFIFDSLHRSEQDGKLINAIAQILNLNEIQINVHPHSGQQRDTYSCGYRVCSFMKYYQEKQFQENVQYKYKEDDMICILKDVINPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF61</accession>
    <name>A0A8S1QF61_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1570039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000162">
        <property type="protein sequence ID" value="CAD8113886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FLAYECN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="323" mass="38982" checksum="B04320BE0DA9FDBB" modified="2022-10-12" version="1">MQILRVDEFSDFLEKTLNLKADCYLASLFKQFGRFFNIAKRMIELLIELDKHFEQENRSRTLINNAIALLNQFFIEHVSFVQWSYCEQFIVQGNERITQIRANIHPIFHQFDQTKIKKSLCEVLLHLQEKNTQLLQQMTLLSNEVNLHQIILKQKEEQYKKFNDNIEYIKQYKQTEDPEFKKYFALFIKLQPLQQSKILYELYKTIILCLINCSNLLCLLEIPIENSKMVKQIGKKNLKGNTHEVQLLMKYSQILVNKEQIWNTLNKQHYEDLAETIKLLEKQIFYXTSQLSYFIANFHLFQWITYNLQNYNQLNNREQKEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFK1</accession>
    <name>A0A8S1QFK1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1550114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFK1"/>
    <dbReference type="OMA" id="EYHCERC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="427"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="434"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="463"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8113547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="477" mass="56665" checksum="2BDF1343F4300031" modified="2022-10-12" version="1">MKQEFCLIEGHDKEELNYICNELQCIDLKNNKLLCQKCLCQHRGVNYNVLRGMAINNYNQYIQILQSDIDENENLKKSVEQSIKEIQGKIMKMLSDLQKSIFTFVDQQSQKLNTNKTKAQQLMQDSNPSLDIYIFFAKFSDKIAQRKQEITKQQVKKIINDLVDDLNQKLEYLKQQDINEYKIIEIELQSFQIESIFQPKLPYSYCNQHFTTKSSICIDINCLQSSDLDYHCLRCCKTTHVEHFKNDLLEEYDKLKKEQKIKQNFLVNEKQKIKDNTQKLVNDQINQLKVEQKEQYLKYETTIENIKSLQQQFEINLSVQDTKYVFGDLLLQFITTKEEELQLQLNHVQKNLEEKLKCCKMQDKQTFLEQCKQLQIKQDEVFKLEKTVQELQSIVDKKDQLLKTQSSENILNLIYTNTQQIIREKDKQTKITEQQSQQSQQQQQSQQSQQSQQSQQSQQSQSLRNQILNPNNKKLPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNZ4</accession>
    <name>A0A8S1QNZ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2020010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000211">
        <property type="protein sequence ID" value="CAD8117523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNZ4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="41" mass="4945" checksum="892A03275DA849C1" modified="2022-10-12" version="1">MLHEIQVKNQNQYNNNIYMFLPQVQKKLESYICFLSPRASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQ49</accession>
    <name>A0A8S1QQ49_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1750006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000184">
        <property type="protein sequence ID" value="CAD8116570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQ49"/>
    <dbReference type="OMA" id="IEWSDKA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF13">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="61"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="95"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="129"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="163"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="197"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="231"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="265"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="299"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="333"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="367"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="401"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="435"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="469"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="503"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8116570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="63245" checksum="64EEB8ECBCC5988E" modified="2022-10-12" version="1">MIPINQDVKQASQYQQQNPNYEIEQLEIQNFENQQHQQTQSQAKESSEVVEKLNQIEFNNLENMINKGQHLQQMGQYQKAIEFYDKALVIRPQFANCLNIKGVCLEKLGQYEQAIEWYDKALAINPQFVGCLHNRGVCLYYKGQYEQAIEWFEKALAINPQFVNSLNNKGVCLEKLGQYEEAIEWSDKALAINPQCVNSLSNKGACLQKLGNYKQAIKLYDEALVINPQSVNYLNNKGVCLEKLGQYELAIEWSDKALAINPQSVNSLNNKGVCLEKLGQYEQAIEWYDKALAIDPQFVGCLHNRGVCLFYMGQYEQAIEWFKKALAVNPKYLNSQIRKGQCLEKMGQYQQAIQCYDKALFINPQSVNSLNSKCVCLEKLGQYQKAIKFYDKILTINPQSFNSLNSKGVCLEKMGQYQQAIQSYDKALIINSQFEGCLHNKGVCLEKMCEYQQAIQQYDKALGINPQFLISIEGKGVCLFKMGEYEQAIKWFDKALDINPQAVSSVKGIGFCLEKMGQYQQAIKYFHIALAITPQYEQQYKDIIQQLFQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQ90</accession>
    <name>A0A8S1QQ90_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2150004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000224">
        <property type="protein sequence ID" value="CAD8117889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQ90"/>
    <dbReference type="OMA" id="CFINGLI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="32708" checksum="D3A6C8A0A5603F40" modified="2022-10-12" version="1">MFLINQEKQFVWEEGTQMLRAIYWDISVKPSEQKTTTLQITFTNNQEIQYSRDGSILRVDLIEDISRKPEILTNLDQIQNLKWWGQYGKLNQKVGKWTATWNGATMKNVGGYYSNTGNKQGQWITLIENYWSQAKVYEVGEYQNGIRKDNWKYIYDNKKIGGGRYNYEGQKHGKWVELQKNFQDHIQVKYTGEYKNGQKIGIWDIEYRNERRNHFIKMQKCFINGLIAVVEHIMKLVMDFSQGLRKEKQIYESNQPQSIITILFPDQFVVQIEMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QRI1</accession>
    <name>A0A8S1QRI1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2350008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000244">
        <property type="protein sequence ID" value="CAD8118359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="303" mass="35167" checksum="5328CF5C618C299B" modified="2022-10-12" version="1">MVLYSSSAHFIKGGTQYLYCFILISSIPNLLESLQISSLCIEFEFLKLFVSLPQKDMLYXEYKNNKKVGKWEIFWNNEEDIKQIGGGFYDDGGDELKFGKWVEVSDGFHVYSQVTQVGEYKLGKKVGRWNICYSDSEGNKTIGGGLYEESGTEIKVGNWLEISDEFTSYIQCFCFSGGGTYDEEGYGIKLGNWVELWDEFQILSQIMYNGEYKNAKKVGKWDLLYRYYDESEFKLIGGGQYNSDGIKDGYWVEINNGFQWTSEIIDDGEYKKGKKVGKWSQQYRDWNKIEEGFIKIKEIIYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMH0</accession>
    <name>A0A8S1JMH0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">tRNA(His) guanylyltransferase</fullName>
            <ecNumber evidence="3 11">2.7.7.79</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">tRNA-histidine guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0040130</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 11">
            <text>a 5'-end ribonucleotide-tRNA(His) + GTP + ATP + H2O = a 5'-end phospho-guanosine-ribonucleotide-tRNA(His) + AMP + 2 diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54564"/>
            <dbReference type="Rhea" id="RHEA-COMP:14193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14917"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:141847"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.7.79"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the tRNA(His) guanylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.79" evidence="3 11"/>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMH0"/>
    <dbReference type="OMA" id="WKQHTEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008193">
        <property type="term" value="F:tRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025845">
        <property type="entry name" value="Thg1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024956">
        <property type="entry name" value="tRNAHis_GuaTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007537">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729:SF6">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04446">
        <property type="entry name" value="Thg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14413">
        <property type="entry name" value="Thg1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028980">
        <property type="entry name" value="tRNAHis_guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNAHis guanylyltransferase catalytic" evidence="12">
        <location>
            <begin position="6"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Thg1 C-terminal" evidence="13">
        <location>
            <begin position="139"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR028980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8042911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="290" mass="34457" checksum="30D5E0BEE1C7160E" modified="2022-10-12" version="1">MANSKFEYVKQFEQMQNLLPNTYIVVRIDGKGFHKFTKYYDFQKPNDEQGLKLMSFSACVVMETFPDIQIAYGQSDEFSFVLKKDSELYCRRSDKIATCICSTFTSIYTLNFEKFMKKPLKFPYVPIFDARCVCYPDLQNIRDYLSWRQVDCHINNLYNTCFWGLVQKGMNKQEAEKTLAGTNAGDKNELLFSRLQKNYNNEPEIFKKGTTIIRHPTNEKINLKVELDVDSQEQQKLNKKQKKKLKYKELNQQELNQLPNQQKEDLQVQEFYLDIIKDNFWIENKTYILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMK5</accession>
    <name>A0A8S1JMK5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0040190</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMK5"/>
    <dbReference type="OMA" id="CICILVG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15845">
        <property type="entry name" value="SNARE_syntaxin16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000081">
        <property type="entry name" value="Syntaxin 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF83">
        <property type="entry name" value="SYNTAXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="74"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8042971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="171" mass="20226" checksum="346A50E5297D62D6" modified="2022-10-12" version="1">MIRDIEENLQIMETDDCENDLEYKIKQNAKMCLGISLKEVTPQLRNYQNQMMMQVQLERPSQNLKVDFEQEYFETLILDRNDRIKQLGEKLKKMNELFKEMNRLVIEQGTLLDRIDINIDQTFTRIKKGKEQLVQANTKQETSDRAQKCICVLVGLNIFIALLFVIKHTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMW3</accession>
    <name>A0A8S1JMW3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMW3"/>
    <dbReference type="OMA" id="MPLFICM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8042847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="32954" checksum="426DA9AB95AF2896" modified="2022-10-12" version="1">MNLQSLPQEQQSLQGFINQIPFGIKLVTIITFVVFLINTLFDDQFTKCFQDQPLKVFQEYQIQRLFFTQFIESIFGLIIMPLFICMNLSDLEKTIGTVVFLLDFFFKNFMIQVIFLILSLITLNNIKPSQGLWNVYIVYISLQCFANPDQLRSLFFFPCVVPSKYIPYGLVMIGLIMNQNIDSIAALILAYVEANYYNFMIFRPSQHFIKRLENSFLFKKAMSRQDFFVMTQNLQGNPSTQQQQLPVRQAEEYQGKGIQIGGSTNFDNIVIDNNDQFQQDDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNK3</accession>
    <name>A0A8S1JNK3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leucine rich repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0040188</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNK3"/>
    <dbReference type="OMA" id="AYNKMNH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22710">
        <property type="entry name" value="X-RAY RADIATION RESISTANCE ASSOCIATED PROTEIN 1 XRRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22710:SF2">
        <property type="entry name" value="X-RAY RADIATION RESISTANCE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="451"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8042969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="59483" checksum="6446130362C901B1" modified="2022-10-12" version="1">MKNKANSQIPFPIAYNKMNHFQLPLLGKKKRKAHTKNPFKTKIYYIPDDTVMRVKCLDGFLMINVTNNDNPHEIIRANLSRLRIQSSKPEDFQYFLNLQDLDVSENQLQLEDLTSFPALKILKICANNIKNIEITHKNTFEQLQHLDLSFNYLTKEAISSLKHITELKELYLVRNELLELPTDMAEFTKLELLDISDNAFQSNQTASVLWEVLGQIPELKTLNISKNFLRGIHTERLVVGYFNKLETLDFSYNIAENQHNLICARNFTNLKKIIITGNPFGITRDHQGLEMEIHARTGGILINEEIEKSYLKKPKVKKPPCKFEQLKRVENDILKLPAIEQSKDGFLLQNEEDQSQIDENQEDNMFVTENQQIKQQQQKIQQQYQLLNNNQKPQSYEEFKKLAQEILGDSKEYDQNQNTSGRPVLDLTKAYQQLKSVINKPEVVDYEDYKEPNYMKPTASIPRYKYQQVDDPEDESIQKRTDNNINSEENEDFNLELLQQGQNQPNIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQ97</accession>
    <name>A0A8S1JQ97_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0080279</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQ97"/>
    <dbReference type="OMA" id="HHEQTET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026306">
        <property type="entry name" value="RSBN1/Dpy-2/CEP530"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354:SF11">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354">
        <property type="entry name" value="ROUND SPERMATID BASIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035736132" evidence="2">
        <location>
            <begin position="17"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="334" mass="38511" checksum="A04DB3501C936CE9" modified="2022-10-12" version="1">MNKILLSLLLVSVCLCQEETLIQLENGVSLTNLNKLMTMDFNKLNCQANLLQMTHQLKMWAELYQDEKVIHHEIRLLSTAHKILNQQKFNRLRNTNTVQRAKRVLAALRHNTHKKQHTIYQQWRVNEIDQLKHSIQLLQTSTTEDESKQCCDRIEQLINKFLDERKHIAKQCGNADVTINIINDAEGKIQIINKKCHEKDPDVKINRVDSIDKVIVGQSQQQKKEEQIVVTEQRQEQVVEEHHESQVVEEHHESQVVEEHHESQVVEEHHEQTETVTEETVEEEVEVEETVIEETVTKTTSSKIVEIPDDDDDVVEGELVSAQGVGASGEGGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQI2</accession>
    <name>A0A8S1JQI2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0080382</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQI2"/>
    <dbReference type="OMA" id="VKYKFNF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF514">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="117"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8044813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="312" mass="36028" checksum="32C942A357A864C3" modified="2022-10-12" version="1">MDIDVDKIIEKLLESKGAKQGKAVNLTENEIRSLCVKSREIFLNQPMLLELEAPIKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGRQSLETICLLLAYKIKYPENFFLLRGNHECSQINRIYGFYDECKRRYNIKLWKTFTDCFNCLPVCAVIDEKIICMHGGLSPELTNLEQIHRIMRPIEVPDTGLLCDLLWSDPEKDMQGWQDNERGVSYIFGPDVIANFLKKNDMDLVCRAHQVVEEGYEFFAKRQLVTLFSAPNYCGEFDNSGALMSVDETLMCSFQILKSQEKRNAPQARPRTPKYVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRT9</accession>
    <name>A0A8S1JRT9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRT9"/>
    <dbReference type="OMA" id="HNLDTRG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="223" mass="26482" checksum="A15CFDA77D1D9B22" modified="2022-10-12" version="1">MINIQPRTRQEREALRDKDRIEKSRIDLRVGFEARGLGVGTLIHQAPPQSTLYVPENERFDKDFAVADKKQREHEVWQREKIIERKRLEGLDRETRKWDYQEKVETKDQVKLMSHTQQLTQGKRNSNGLAYNPITLKYDNSEQGNQLRQFDDKAKVRQFVRAHNLDTRGNTCFNILTGEQRGGVDQIVPNHLRSNYQQRLREADEQQNIKHYAIQQQLLNQYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWW2</accession>
    <name>A0A8S1JWW2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inorganic diphosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080445</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWW2"/>
    <dbReference type="OMA" id="LYANEQK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000010">
        <property type="entry name" value="Probable inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="30916" checksum="3FCF8E1D96379138" modified="2022-10-12" version="1">MNILRIVYRYSQSFSYRLSEQGQGFSYKINLHCNDTIKSFWHDIPIYPIKDQYNIINVGIEIPKERLAKFEVSKTIKYNPIVQDQKKKKNSDEKELRYYAQFAPFNYGFIPQTWENSTVDLHDGFKGDDDPLDILEFSIQKEYRPGDIFQAKIIGAFCVLDQGEIDWKILVLNTEEADKFKINDFHDFEKQHSDISRLILNRFRYIKTFEGKKENSILFNNQIFDANKAIDIVKDGHKQYQDLLKDPKLNKKREEFQITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYD7</accession>
    <name>A0A8S1JYD7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Calcium uniporter protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0120082</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCU (TC 1.A.77) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JYD7"/>
    <dbReference type="OMA" id="MIEFEYP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015292">
        <property type="term" value="F:uniporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006769">
        <property type="entry name" value="MCU_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039055">
        <property type="entry name" value="MCU_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462:SF10">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04678">
        <property type="entry name" value="MCU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium uniporter protein C-terminal" evidence="16">
        <location>
            <begin position="158"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8047772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="343" mass="41261" checksum="4034994B31659D86" modified="2022-10-12" version="1">MIRFAKMFRGLNHRSQYWHIVSKRNVQITFKDDLLVLTFPLIMGSKMFFAHQQYSFLTLQEQLQLEESTKVEFNLDGTDLSLSSNLFNYILNDKKNKAIKLVIQDEEFTMTPAYQLVEAKMLNQKTQLKHSPQQKYIQMVNMGMDPSHANTLSWFLNSCNIQQNLTEKEIVQVMEQGLSILKQPTKDKLYELHHLINILEAEICKQREQLDVMIAQAEQTGYRWLTLLFVLSLLQIGAFYYGIYMVDEWGWNVCEPVTYTLQCVTVLLGMIFYMRYRRQREIETIRWAFMFRKQIKERNWKDRWNHLNTLISLKEEQKRGLELRKTILYNRMNYHYYVQQQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYQ4</accession>
    <name>A0A8S1JYQ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JYQ4"/>
    <dbReference type="OMA" id="FTWVICN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF2">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="1">
        <location>
            <begin position="167"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="367" mass="43129" checksum="E5356993187557D3" modified="2022-10-12" version="1">MQQQKCYVIPGLLNAGLLNGNQPIYIFILNQIQDMKEVSLKMNSYLQTVNAFHPFTWVICNQQTLQLEISEVKQILNQFCKNIPLQQFQRIDKLIIISDTIQLQQIINVIPSAWSTILTNKIHLVLRLNQLINHNIQLTQYQVEFLSRINNTYQEEIPFYGRDISEYPLANTGVPECLEAILQYYDRHDDYLKKQGVFRISGSVQQEQKLIQQFQIRNYSVIDDYDPDVVSTVFKNLLCQLKKPIFPFEMYDILKETQINTSKEKLLEMFQVFFAYMPEVNRKTTKRIAELLFHVADYESENLMGLNNLSIVFAPCFLRPEQTDLSDLQGAKIVVHHFNLLVQNVEFFLQEFRINRNSIPQKVQHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYT4</accession>
    <name>A0A8S1JYT4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0120125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JYT4"/>
    <dbReference type="OMA" id="DECKHNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="226"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8047813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="386" mass="46899" checksum="8144902F159BD0F3" modified="2022-10-12" version="1">MQDIRQMRQKYESIKQSNPNTPKKCLTPTISAQYIKNSPGLQMTPPSNPRLKKICNNCLNQSLMKQKEHRVQQQRQEDQKLFQQVKQSIDQENQYRDQLADKNRRKFLTYHKINGDLIQLHQQRIKTEQQNEQVEVANFFKQLEKDDKLRKIKQIEKKEQLQQYYLRDLKNQIQFKEEEKSQQKLQNNKSDIIESQYWTSLENEQIHNQQIKAQQQRQMINYWQYTLGEKQKLKQQQQEINKIEQEQLKYLQKSNDKLIQATELAKKQQIEQFKQEIQNQVKINEQKRKNEENKKQQEYYIMIQQKQQEEQREKQKQLDKSIEKLALIKDIEQQINFKQKVQKDDKERELSENEKKLLIQETPIKLVCCDECKHNCPSKVITNILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZI8</accession>
    <name>A0A8S1JZI8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Actin, cytoplasmic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0050241</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZI8"/>
    <dbReference type="OMA" id="RNDYDEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8043588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="359" mass="41721" checksum="318A4379FD3FA352" modified="2022-10-12" version="1">MNNIYSHSIIIDNGSAFCRVGYSSDNIPKYSFPSPVLRQKYSRNQISEEDYLIKYPIQNSKIVDFDTMESVWHNAFYNYLNTIPDESSILITSYLSTQRKEKEKITQIMFETFNISQFNIQIQSILSLISSGKTTGFIVDSGNDTTYFVPIYQGYKLNINDNFCNIAGKSCSIYLNHLFTQSNQKRIENLEILDYIKEQYCYLTQETQQELQNQLQDPFKPIVYELPDGSHLQFGIQILQSPQIIYNPNIYFNTSLGYHQLALNSIEQIESDLKIPLLRNTMISGGNSFIRGFSERFIFEFNKISDQRYFEQLNITKSQKFSAWIGGCIIANLAQNSNLWISRNDYDEYGPNIVQIKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZX4</accession>
    <name>A0A8S1JZX4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZX4"/>
    <dbReference type="OMA" id="NYCKINC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="262" mass="31608" checksum="EB39647304DDC4B4" modified="2022-10-12" version="1">MGEFVNIDFTKKNAQPMDFQVDAQPINIYMKIRIDLRKNLQIPRNNLNRKNQKYKIGPNNKKTNNNIVVERIQRYMFRRRKIKKKIKNYCKINCYNSSRIIIQSLIKRLNELLDDVVKIEIRSHQRLQNQEIEEEKAQQLIIQRLEKEKKGNKKNEQNQKFMEKWYLDQLKNIKIMNSYQQRKNQMLKLKKGQKNYSEEIWYLLKQEIKNDQLKSSLRHVTQGDLLVKSEFNGCVRRGLIEPKSKSNKLSKAKVPLFKIKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1L0</accession>
    <name>A0A8S1K1L0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">EF hand associated type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0110113</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial Rho GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1L0"/>
    <dbReference type="OMA" id="HETTWGI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003397">
        <property type="entry name" value="Eng_C97 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000011">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000737">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013567">
        <property type="entry name" value="EF_hand_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052266">
        <property type="entry name" value="Miro-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08356">
        <property type="entry name" value="EF_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="537"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="EF hand associated type-2" evidence="15">
        <location>
            <begin position="224"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8046810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="568" mass="66001" checksum="FBFA84A3F21635EE" modified="2022-10-12" version="1">MKKRDLKLVLIGDSGVGKSSFVSALINQISNKASVLDKHQPISLPPDMLNHPECITTLIDTKCAPHQLPEEVQIADVILLMYAIDDDGSSERLKRFWLKELRDKEYKQPVIIVGNKLDLLGLEDDRDYHRIFKVIKQLVKDFNSVEMGIECSSIKQQGIYDVINCAQRSFLYPLAPIYSIAEKSLTEGFKKALTRIFRICDKDGDGVWSDAELEKFQKKVFKRQLDYSDIAGIKDMIEEELHDNSNKKVITLEGFIALQKRGIELMKIQICWTILRFFRYKDDLTLDESIFTNELIFDQDAGQTVELSDIALSKLKQIFEIRCNSISQLKNTLNQKQFDDIFYPVMYRTNFPHLNQYYPQEQDIINLTQWLAMWNAFSFFNYKEAYKLLCYIGIEIKLSDTFKEQNRKNSWSSVQKSIDRKVFHIAIVTRKKHGQLEKEFGNLPLIITTIQNKTYAISLYDELEAEQQIEKRRLSIIDFLLIDQNCQFDTAQLIPKLSYNDQKPFWDQISQINDILHQNPFGYSESQIQLLKKQNSLPIVELASVITLLAFMLTGGYFLGKKVLLKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1N5</accession>
    <name>A0A8S1K1N5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0100181</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OPA3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1N5"/>
    <dbReference type="OMA" id="YEIYEAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010754">
        <property type="entry name" value="OPA3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499:SF0">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07047">
        <property type="entry name" value="OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035858924" description="Transmembrane protein" evidence="4">
        <location>
            <begin position="17"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8046096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="23159" checksum="77FF010C3F3C34D3" modified="2022-10-12" version="1">MLPQLKLISLVVRVFARPIITSKKEISIQKVQMSKQRFITQQMLNLGNAYYFWDQKIDRIYLNQNLKVEYDQLNEDEALKLGTEFFLEILIYTILILVSSFEIWKNYFEYQDRQQNINNTIRVILMLKQNLQYQINIIKEQKLKDLNDIQQVYEIYETNLKSFQNKKDKAIKQISIIKQLHIQDTKKNNQDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2L5</accession>
    <name>A0A8S1K2L5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="3 12">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0130069</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9 12">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of a large and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="3 12"/>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2L5"/>
    <dbReference type="OMA" id="WLNIKTE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01679">
        <property type="entry name" value="RNR_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.70.20:FF:000010">
        <property type="entry name" value="Ribonucleoside-diphosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573:SF6">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="13">
        <location>
            <begin position="22"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8048764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="833" mass="95071" checksum="B0BF2FAE9810FA7E" modified="2022-10-12" version="1">MDQDHDYSFEHLQELAKQKNISYVLKRDGGKQNIDIQKIAERLQNLASNLQHININLIMWKVIQGMYEGITTVQLDNLAAETCAYMNLVHPQYSLLAARIAVNNLHKETSESFSEVATKLHSFTDKYGRPAPLIADDVYKIIMDNKDIIQKEINYERDYQYDFFGFKTLERSYLLKEGKKPIERPQQLLMRVSIGIHKEDLQSAFQTYHLMSQKYFTHATPTLFNSGTPYPQMSSCFLLDMVDDSIEGIYETLKRCALISKSAGGIGLSVSKIRSQDSYIRGTNGISNGLVPMLKVFNDTARYVDQGGGKRKGSFAIYLEPWHSDIISFLQLRKNHGIEEQRARDLFLGLWIPDLFMQRVKDDSDWTLMCPNECPGLQDCYGQEFNKLYTEYESKNMGRVTMKARQLWQEIIDAQISTGLPYMLYKDSCNQKSNQKNLGTIKSSNLCTEIIQFTSPDEIAVCNLASINLQKLIKEDKTFDFDKLLEITKLITTNLNIIIDLNFYPVKQAEYSNKRNRPIGIGVQGFADALQRMKIPFDSEDALELNAKIFETIYYGACQRSLELAQQLGPYDTFEGSPAQQGILQFDMWGVNPKLYDWNTLKQNIVKYGMRNSLLVAPMPTASTSQILQNNESFEPYTTNIYTRRVLAGEFVCINPHLVDDLIELNLWTPQIKNKLIMNNGSVQNIDEIPNNIKQLYKTVWEISQKAILNLATSRAPFIDQSQSLNIHMAEPTMSKVTSMHFYAWEKGLKTGMYYLRSRPAADPIKFTVDVEALLKEGGQIKISQQQSKQELIDESASKKIVTNEGKTVKKGKPQIIVTEDGDEVEVCLNCGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3P6</accession>
    <name>A0A8S1K3P6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0130195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3P6"/>
    <dbReference type="OMA" id="IIPKNIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="244"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="527" mass="63044" checksum="9417E2E53811EF79" modified="2022-10-12" version="1">MSQHITNNYFQHFLKNDSQRRIKTLNSINDTNSTKIKKVIIPKNICIDKETLYEQLYHEKLRSKKLSVENQQLIGVLSQLESNINIMDQTDQRKMLSQSFLKIKQQDQEIQTLKESIQYKVQVELKRQIQDLQTQLLKYQLSSKFEGDQYELIEDNVSLLNKLESQKQYIIDLEKTRSEYILIKAKFNQLQQVLKLKEQQIQRFRDRDPLSEMNLLRNPTKNENLLMNELNVKKDEIQLLTYKLDQSQTMIQVTQNALTQLNQESKHKIQILENEKRILIERLEKLQLDYNSILEKQKQMDKYLSQFQKKKIQTSPFIYGEDSLFQSATQLPLSPTATSNDQLIVKQVRKQQIEQIVLELKLSLRKKRITLQEAENILFSNENDISINEIEKKLKEDPFTIKSSTLLARYLVEDYSEKEFVYDPDLKVPQAQVRSIFKNLLQNYTLIFDDQVIETVKIFIQHYLQDYCQKKSLSIINQDRIIDCMQLYEVKWNGKHSDYLQQEYYNKYSKFQEYEINNLLRLFEDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K736</accession>
    <name>A0A8S1K736_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">T-complex protein 1 subunit zeta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0100252</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K736"/>
    <dbReference type="OMA" id="KNAIEDX"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03342">
        <property type="entry name" value="TCP1_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000038">
        <property type="entry name" value="Chaperonin containing TCP1 subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000058">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000004">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012722">
        <property type="entry name" value="Chap_CCT_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02347">
        <property type="entry name" value="chap_CCT_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8046238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="58476" checksum="F94E746A3B204D1C" modified="2022-10-12" version="1">MSSVQFVNSKAEVLRKYQALAMNINAAAGLMEVMKSNLGPKGTLKMLVGGAGQIKLTKDGAVLLSEMQIQHPTAAMIARSATAQDDIIGDGTTSNVLLIGALMKQAERLLAEGIHPRVITEGFELARKEALSFLDTFKYQQIDKAVLINVARTSLISKLTPDVANQIIEIVVDAVQIVHVPEKPIDLFMVEIMHMQHKMGAETELIRGLVLDHGARHPDMPKFVKKCYILNLNVSLEYEKTEVHSGFFYNSAEDREKLARSERKLTDDKCQQIIDFKRKVCEKNGYGFAVINQKGIDPVCLEMFAKEGIVGIRRAKKRNMERIAKACGGNSVNAVEDLTESDLGYCEVLREYTLGEEKYTFIEGVQNPTSCTILIRGPNEHTIAQIKDAIRDGLRAVKNAIEDKCVIPGAGAFEIATSVHLQKFKDSVAGKAKLGVQAFAESLLVIPKALAENCGYDVQETLIQVTDEYIKNNIPVGVSVNEQGFIAPIANGIFDNYCSKRSWLNIAPTLAQQLLLVDEIMRAGKQAGGAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KBM4</accession>
    <name>A0A8S1KBM4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0140357</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KBM4"/>
    <dbReference type="OMA" id="TATETRC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="10"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8050172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="370" mass="43108" checksum="3AFAC6B7D5F06A12" modified="2022-10-12" version="1">MYQCVLNYTINKSILLGKGGFGSVYLAQNELNQESLQSSQNNYKKLKQLVDRELSIMSTLDNQNIVKMAHSYFDGKNIYIFMEYCNGGNLKKKIRELQSIYPCPKFYQQKYEKLAQEVFWDIIDSLNYLYSKNIIHRDIKLENVLIHNGKYKLSDFGLSKFLSDIDEEPMSSILGTPCYQSPQLLRQQVYSPKTDIWSLGVLLYELLKGGELPFRGFNTQDLLKDIEIKLKTNYIQNQLLSVENKLLQYLVSKMLVIDEESRLSLKDLLLKVNQFRINAQLMQKDNITATETRCKPYRPEKITSKKHSCQNQEIINNKQSIPIRFIQTYKEKKLEATFISKILDDKLSSNILILLALMLFIALMQISIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCE7</accession>
    <name>A0A8S1KCE7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCE7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="132" mass="15314" checksum="DCD3EF3708AB3675" modified="2022-10-12" version="1">MEHQFVGSSLNISPFRYQLYYVWNRQGHFGTSTSIPNSTSIDIQFSQAYEINQIIVWFYDIDGRNHCFKIIAFDQYNQETELFQGLNLIGIVKISFQDQIVQSLKIVLLHGGTTNDLNIIKISAFYAHRSMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KF36</accession>
    <name>A0A8S1KF36_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0210139</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KF36"/>
    <dbReference type="OMA" id="QRMKQNK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20908">
        <property type="entry name" value="SUF4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23215:SF0">
        <property type="entry name" value="BUB3-INTERACTING AND GLEBS MOTIF-CONTAINING PROTEIN ZNF207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23215">
        <property type="entry name" value="ZINC FINGER PROTEIN 207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="118"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="125"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8053858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="171" mass="20584" checksum="2E51D8A0563751C3" modified="2022-10-12" version="1">MGRKNRDLSYLKPFCFYCDKTFKNEVYLHQHQKAKHFTCQRCFKKFSSCDSLKNHVESAHHEVLTKIPNATGDRSDIKNKIFGMQGVPRVEIEKRIRKGAEEYWTKILSAQYQLRRKENKQRMKQNREISKKENQQDVKIDNEKEEIEQHEYNPEAKPISFDLDYEQLQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KGU2</accession>
    <name>A0A8S1KGU2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KGU2"/>
    <dbReference type="OMA" id="YINDCRT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="167"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="386" mass="45858" checksum="9D26A8782CE952BF" modified="2022-10-12" version="1">MLLTRENIEFYTQRRKQYCEDFLKLTNQFKPKLELLQKKLIASKQVIDGPSKSIPHQQKIKKVNKVLASLSKSKEVQQKIDKEMIRCSCQYKLDEVPNCNFNYSQTCNKIQQAMIPLIQNLQIQIQDKQEEILSDLDCADLYIKRDEFQKMKHKSKRLQQIIEKLQVMELKQEEKVYRESIKKLEKLDQAEDYDETLPYSKKALMSKKKIEVQALMDEERKQRKQFENERIREAIEEQKQKYQSLDNKIKTHYRKMGLQKRKLESMSDLREYINDCRTNSQIKLDSQNNLKQQQQLKSILNADKRFSITLFSTQMNSQNITPIACEINGLTAPIISNVNTQRTIINNFGKKTSQNFNKIKRRYHTLGQRLENGSNFDFQDNKLILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KI54</accession>
    <name>A0A8S1KI54_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0220238</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KI54"/>
    <dbReference type="OMA" id="KMLTTKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035806932" evidence="2">
        <location>
            <begin position="16"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8054849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="345" mass="38678" checksum="B9A044F0C3D94C7E" modified="2022-10-12" version="1">MKALILLVLALSAFAFTEQMMSLEELANFNVKTMDCSRSDQFSFIEKQMKQWEDLLIHQKAISHDIKILEQMEKMLTTKHHSFLEAQVSVSGKKLLKKLHKLELPLTKSKIGLNQVKVLREQCHALDSENHEDRAAAKKELCKLLREYINSLNNCRQQCRSTPITVIKIKGQIKDIEIIRGGCTSNGSQTGVKVTSEDDETHEITIHHHHKGKTTSETTQAAGSGNQESETQAESHEGQESQNEQTSATETGETGEEEVTEENTETAEQTNQAEEEAAEETTEKGEETTEETTEETTETTEETTEGTTETTEGTTETTEETTETTEETIETTEETTETTEETVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIW7</accession>
    <name>A0A8S1KIW7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0210202</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KIW7"/>
    <dbReference type="OMA" id="TDENQHS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35245">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35245:SF2">
        <property type="entry name" value="DISINTEGRIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="4">
        <location>
            <begin position="907"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="198"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="207"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8053925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1139" mass="132623" checksum="D121077BD7A59AF8" modified="2022-10-12" version="1">MSSEMKSDEDQIAKTKENQIRDWKEQIKNKGYQYIETYQFVSKGKNFNQRNYGVTVDYRFLRVIQQLDFLESQQIQFYMNIFKDVYKDVKLAAGFCHFFAEFWTQSTESLSFNINPNFSWNTQISNFNQYQVNRNQDYYQIQNQDFYSKQNDMIENNKHDENREIIQQKLKLNNNQETNGNNNSSIIQGIDSQQTDEIKNQTDENKNSSIIKGTDSQQIDEIKKQNDENQHSSIIKGTDSQSNDEIKQQTDENKHSLIIKGTDSQSNDEIKQQTDENKHFSIIKGTDSQSNDEIKQQTDENKHSLIIKGTDSQSNDEIKKQTDENQHSLIIKGTDSQQIDEIKKQTDENQHSLIIKGTDSQQIDEIKKQNDENQHSLIIKGTDSQQIDEIKQQTDENKHSLIIKGTDSQQIDEIKKQNDENQHSLIIKGTDSQQIDEIKQQTDENKHSLIIKGTDSQQIDEIKKQNDENQHSLIIKGTDSQQIDEIKQQTDENKHSLIIKGTDSQQIDEIKKQNDENQHSLIIKGTDSQQIDEIKQQTDENKHSLIIKGTDSQQIDEIKKQNDENQHSLIIKGTDSQQIDEIKQQTDENKHSLIIKGTDSQQIDEIKKQNDENQHSLIIKGTDSQQIDEIKQQTDENKHSLIIKGTDSQQIDEIKKQNDENQHSLIIKGTDSQQIDEIKQQTDENKHSLIIKGTDSQQIDEIKKQNDENQHSLIIKGTDSQQIDEIKQQTDENKHSLIIKGTDSQQIDEIKKQNDENQHSLIIKGTDSQQIDEIKQQTDENKHSLIIKGTDSQQIDEIKKQNDENQHSLIIKGTDSQQIDEIKQQTDENKHSLIIKGTDSQQIDEIKKQNDENQHSLIIKGTDSQQIDEIKQQTDENKHSSIIQGTVSQYNDDIKIQTNFNQASQKLQIQDSKQHDFNNSNIYQNETLNNQNFNQQHIQQTQQQPVQQTFVQPQNQINAQPPQKTFTFKIINMSIEEPVKKQKFQINKLPDLLNQVAQMNGLSSKEDLKNQDFLYFPINLSQAHWISVVVNLRDKIIYYFDSYYEQVEEDVREGIYIILKSLDFNRNIFRFETKWNKQYNGYDCGIFILLSLLHTFQGDLKYSYNQARASEFRNRILYDLAMVASQVNLSKELFESILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQ81</accession>
    <name>A0A8S1KQ81_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQ81"/>
    <dbReference type="OMA" id="QSSHAMT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="352"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="426" mass="49734" checksum="1EC73CEB8673D9FC" modified="2022-10-12" version="1">MQLPPQPAGVMTYYSDQYEPQCPLCQSGEHTSQTESNFVQSSHAMTGQQIRGRVVYTSPERIVSRKVVEGVALSPMQLRQQQAITPPRMMPITSQMINTPPIIQQKSPIVVQQSPVIVQQAPIQLQPSPIAINNSSNVIQQAIYQPQIPNMQLQYQVQPQIQQAQITQTNINTQQLQQSQLIQQELLLKQKEWAEKYYRQSEEIQQNNLAFSQLQTQYQEQLEQYEALKQAYQLTTQYQAQNFIPSFLQTVQYKVINKDGKSQYNQEEIELYWRHQVFKLEEQMYQLWEKINEKKLKKDLPIKSEDTQKIEQLQLEVGHLEHIQRMKMNSVVILRNKLNDLMYQHDPATEATREYDLQLQQLRQKVMQLSSRLTEVQDEIQMTETQTEAIKSGKGYKFVSVKQTTSQVRQVTNSVRTNQAQDYQVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KR57</accession>
    <name>A0A8S1KR57_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230318</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KR57"/>
    <dbReference type="OMA" id="DIMNNNK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="442" mass="52033" checksum="6D41F88F909E82C9" modified="2022-10-12" version="1">MPKHNPIYTPRFDEVLTELVGTLGEAKWAEITRQMKTIYGLSIPHKPMVQARWRAIDPKINRQIFSAEETVTHWHICVKYKCHWDAVKAEYEAIGQIRDKCFLSQKFYAHFQFKLVDLNRMMGKMVAYNQPKIENMRDTTKKRILEINAREPQSVKDQSAREIIQSCQMLIKLLTYVVDNHKEDYKVITEELKKMITPEKFARVLQNIFIIDLIVLIACNELKPSDETPDFIKMDERKKIKYKKPTKVKRWDEALLLSSSPENTEDEDVLRTEQEINKVTGGTNFTKADIEEIKQSPIYCVCNTDQNQTFYDWYNEAKIQKATLKTDLLVDVMDIMNNNKDKQKSEKTKPVQKRTALLAKKQMKLIEKKKNELMKQDKDDKIKTVRGKIKIDKEVFPGCYTKFFDKEDEDDYEDSQDNPIYSAIDADQIYQIIMQQKQAQDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRX5</accession>
    <name>A0A8S1KRX5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRX5"/>
    <dbReference type="OMA" id="ISKIHDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="697" mass="83121" checksum="EC4BB2903AA5FBC5" modified="2022-10-12" version="1">MKHARFNNQSNSPREIGLFRQCIRFQPKERSPQPNEEDAMIYSKLRRFGLVRDKALKYCLTDHCKIDASQCRHGPPNFKIFQTSLDKQKIIRGWQMVGKLKERSSEKFQKAKGDDGLMQYRNQEEQKKFERLKNYFLGLSNSSKQVKLPQFQMGNGRDHYFELADYYEQIKPLSRTLITDTESFDDIIPKYIREQFRIEPRVQRPGLKRTDEEKGLKSRLKALNELIETYREAGSPNDPSIEISNLIQKLMMKDNVKNFQVQVYQKSKYLMRKEVQLQKRDIVFQAIDILSFSQEKNKEKINNFNKNLINQSQKLLVGQIVNLMELYLVKISKIHDYFLIIFKLLLQLMRETAFSSSPFEKPNKLTSGLFRDCQRFISRRKFTEENQENATVFAKLRRAGLDKKKALKYCQAQHCKLDTTQNEFGPPGYKIFKQNYDPIKQYKGWQLNGKIKLQDQFEYEKISKDINLGLRQQPNEDLLAETHRKTEERCNFTELSRQELIMKLRSLTRDDNRKYNTPIYQLRRHNEIYYELADYFEKQQPPQIKSLVTELEAFDDIVPKKYIKQFYLEQKSTGNKKKNLKHEASEKIQRRSRILMELTALFSPVKSDNSNRRSFYTKQDQMWQVQNDSAITSLLDMDESNNSRNTERNSIFVFRFAKVKQTEEQERINQFQESLKKVIQKNIENINYSKKQNKYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KS18</accession>
    <name>A0A8S1KS18_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0270105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KS18"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="133" mass="15344" checksum="BC2B188DFCE299D0" modified="2022-10-12" version="1">MTSNLNEPFIIVKNYRQQQQEAKKEQSKVKPAPKPKELSKKIAKPKKISQTPQFQQPIAQKLDPQLFSKLLQQHQQQQLMFQQLLQNPQVNQISGQDDDLDQYSPVYLYGIPKTPQNIAIPRGSTGDEDDFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KW30</accession>
    <name>A0A8S1KW30_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KW30"/>
    <dbReference type="OMA" id="TYYCNKS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="118" mass="13642" checksum="0F1CD6B121AD64F6" modified="2022-10-12" version="1">MNSQQQTNPEYFPPPTYYCYKNGHQLTWKSSSGHPCAECKIKNCRSRYWCQQCQEAFCLQCMPPPLFGIMCGAGHPMKQSIVPHHVCDYCSETIQNQAHRCQICDFDICPNCLLQKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KX29</accession>
    <name>A0A8S1KX29_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0230234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KX29"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035725524" evidence="2">
        <location>
            <begin position="19"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8055544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="84" mass="10116" checksum="B40081EF5DE55AB4" modified="2022-10-12" version="1">MICKSLGLFLFKFSISIMQIIKPIKKQHRNYHISVLKIFGKSKSKLGINFLFIRIYIKSVQRHTIILTITTHYNIIIYYIFKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYW4</accession>
    <name>A0A8S1KYW4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0300336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYW4"/>
    <dbReference type="OMA" id="FIIVIEE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="197"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="466" mass="54621" checksum="CE42FFEAECF12FD8" modified="2022-10-12" version="1">MEQVDQIREDYKIKTEGIIQNMQQAIDESFNRIDAQLDQIFNDKFSFIQRSPSQLQSDLPKINTLFQFQNYDYQKQINQEVTSLIKTNIQKVFQKKDQYYQNQQKENQIKWENKIQEKDYQIQQFINKQTIQQSSISTQFNLKPFTYQLIQQNSIKQNELCFVIAINQDCSILVAGCDSQIKVFEFQQEIMKQVQLQSEHGGCVTTLNFMKRSDQFISGSNDKQIIIWKRNQSNSWISQQKLNGHTNLIVSGSCDNQIKFWLKQNEWICSQIITDHNVGVYGLSLNEQQNRVISCGDGKFIIVIEEQNKQWIVIQKITVEINGYRLCFIDDNTFTFQPYAKEYMHVFEMNSTNKYTKTKDIPVKGGSDSCSFPQQYIKSKCILVNKNASNVNLIRKKQNGEFIIEQSIYFGTGSNYGYMTDDGQYLITWDYQTKEYQIGNIKNYELLNTINIIDQLISNQVIAQAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1T3</accession>
    <name>A0A8S1L1T3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glutathione transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0280358</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1T3"/>
    <dbReference type="OMA" id="YFRTIWL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000202">
        <property type="entry name" value="glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000039">
        <property type="entry name" value="Glutathione S-transferase theta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051369">
        <property type="entry name" value="GST_Theta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917:SF8">
        <property type="entry name" value="GH16740P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="88"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8059353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="26245" checksum="A745FC595C3E3D8B" modified="2022-10-12" version="1">MIKLYIDWISQPSRAVKAVLDILKVPHEVKALKIQFDEHKSPEFTAIHPLQQLPLLQDGDFTVAESHNIMRYIIKQRNISTNLYPLTDIRQQTRIDQYLDYHHTNTRRCLHLYHSVLISPIKGEKVIPEVLEKEKQDVAKVFQYFESNWLKGRNYICGDQATLADISACCEMMQLDLIKFDFQKYPITNAWLNRVIRIPEVYQAHHVAFKIIKKQNPSSQFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L361</accession>
    <name>A0A8S1L361_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">PPRIM_AZ9-3.1.T0300254</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L361"/>
    <dbReference type="OMA" id="YTEEWSK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000632">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000522">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="61"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="359"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="401"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="437"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="476"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="CAD8060675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="502" mass="58284" checksum="9627AE30E607F3AD" modified="2022-10-12" version="1">MGNTCCSQDVISNTPEIEIRPKELNNNDKQFSTQATKKSTYLKLSPDLFIQMKREDIFSQYSIGKALGEGAYGQVSLVTNKRTGIVRAMKAIKKDCLFEEEEQRLFQEMHILKNLDHPNIVKLCELFQDEKCYYLITEYLYGGELFDRIQKAKTFSERDAANIMKQILSAVAYCHTKQIVHRDLKPENIIFTSTDDDAQLKIIDFGTSRRFEQDKKMTKRLGTPYYIAPEVLLKKYNEKCDVWSCGVILYILLAGYPPFYGKKDIDIYQKIVKANVPFYTEEWSKVSDQAKSLILKMLCKDAEQRISAKDALDDPWMIEHNQTNLVDQQFLKNLSEFSAKSKLKQALLTFMACQMIQQHEVEDLQKLFKELDTNSDGTVSKDELKKAFQDKIMNKDYFVESIEEKIENLIQQIDINQSGKIDYTEFIIASLQQQRLITEEKIKQTFKILDIDGDNYISKAEFQRAMEGVDDVIWGEFLEECDDDKDGKISEAEFIQIILKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4X3</accession>
    <name>A0A8S1L4X3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0300228</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4X3"/>
    <dbReference type="OMA" id="NDFYKCH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="274"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8060623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="45374" checksum="933E76B6F4E82F1C" modified="2022-10-12" version="1">MITNFIILVYLGSWTLFIILIILIGYLLDKQYKLASISPQINNNKNPGETTFGILPITSKIQSQTRIQQITRLDNNELEDFFNNQGDNSPNLNQDDQLYSNGERNISEQHSPIRFTVDPKQVSSPRINRTPQRSNTLRRTILRKPTIGTFEGIDTVQTTNRRSLRTPGKRLSQIQSDRSSIGDFTPRSNNQKYQQNQPRISQFSNFGRVEAIKQDPNYLIPKPENDFYKCHQFSKIFYSHKEGISRIFMILIIYFRQIICMDVSGLLINYLPNFNFWIIIGITLSCCFLLKISDYYSIKCIVHHHQKLKYVLIMVWISSIGIIIWLIIFLSFDNLQWFIEYLPSLLLDLIVIDPLKYFMIKYCLQEPKKIREPKSKVDVLKVMQQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LD29</accession>
    <name>A0A8S1LD29_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lsm14-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LD29"/>
    <dbReference type="OMA" id="PYTKFPI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025609">
        <property type="entry name" value="Lsm14-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12701">
        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lsm14-like N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="179" mass="20998" checksum="46F26B8DBB3F71AD" modified="2022-10-12" version="1">MITINIETPNHSRYQGLLHKIDTTENTILLHSVINYGKAKRPIKQIISDTEKHNYFVQFPLEFVKPDTVITTNDGKSYKAKLSKIDPYTKFPIFQNIQLICKQCEEGEKKSFDFKSHRVISINKKQQIKMKQSLHFQNKSVDLKSSYPPCITNNYRQMAKFKELTEQIGYRPKLKCKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDN5</accession>
    <name>A0A8S1LDN5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0370002</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8064969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDN5"/>
    <dbReference type="OMA" id="QIYGMAM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF9">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="383"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="20"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8064969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="404" mass="47392" checksum="4C89CA13EBC59190" modified="2022-10-12" version="1">MENLFCQVHPDIPFFDLKKYHQVKLIASSNNRVYLYKFLNQAVAVKEVIFQQLQNEQIEKLKKQYQIQKGYNETIIQIYGMAMQQQQRFSGKFQTIIFICMEYMKYCLQDHIELIRNQQLPINKNSFSNLLKSTVQTHAFLQSIHLCHGDIKPQNILINDKINPTQFKTCDFDNMIQLFHAKLQPCIISTKQYHAPEIQQAIKEGLESFNYNPYKAQVYSLGLCLIQMHPQIDFFKINHITFMLIEQVYGTQIQTLISKMLDPINYNRPDFFEALQYLNRNSIANLSTEQLEYQGNDILKSQNSYFRNTPDMFDTLRRESKYPNQLCDSVDTTSDFLIQGQVNLSSEENNNLYDVQVSTQTGISSNKNQKQCCSSKYINKTNILIFCSILLLLVLFILLLKYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LF58</accession>
    <name>A0A8S1LF58_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0390069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LF58"/>
    <dbReference type="OMA" id="LHDDRGY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="282" mass="33933" checksum="37D1488B9344E70D" modified="2022-10-12" version="1">MDIELQQLDKVKWNSQEDDLLLKLQKHFQNDFQQISSQMNKIFFYNKMTPSLCQYRWRYYIDSNINRSEFNQTEEHSMFQQMKKNDLYTNQFWFELSDQFQKRPPSVLKVCTMKFIIKYLIALSESYVQNKAVKKRYLARIKQFNIDTMITFFRLGKEKQKRTGPPTLLSQGIDQCTQLLLYLEQMISTRGLMSKYDDQSEQASIKFHSMMECLIFIDTIFRIFHKISPYNQGEQAERDKMETLLPSKKSTISLHDDRGYEDQAFEQLNKEDVRLIYSSEYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIZ2</accession>
    <name>A0A8S1LIZ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0390262</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIZ2"/>
    <dbReference type="OMA" id="EHKFVNM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06623">
        <property type="entry name" value="PKc_MAPKK_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000040">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF32">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="97"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8066719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="377" mass="42944" checksum="D993759C294A4411" modified="2022-10-12" version="1">MSKKIKNTQPLPKVNWKDTDMEEELTIKIPHANLINKIIDPRSSPQEFFVPIRFPAQQHMKDADGFFIPLPKEDPKKKLQQQHQFQNKRYQIQLQDLISIDDLGQGSSGKVIKALHRPTNLIVALKAIQVMNDEKFTKQINLELETLVSCDHTNIIRCYGAFLEGAQVAIALEFMNLGTLQDVIKKQGKIPEGMLGLIAYQLLKGLDYLHRTKKIIHRDIKPSNLLINSQGEVKISDFGVSGQLLNTQDQRSTWVGTITYMSPERFLCEPYSSNTDVWSLGLSLLECAWGVFPYPHPGTNETTHSLGFWEIKEYIVYKSAPPSPPDFSQIGADFIARCLQKDPGQRNSAAELLEHPFIKQYEDVSLQYLEGWLNINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LP47</accession>
    <name>A0A8S1LP47_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LP47"/>
    <dbReference type="OMA" id="KNHNDCY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="326" mass="38755" checksum="0A52A938016C2BEF" modified="2022-10-12" version="1">MFSFGYKNLYDPKPIKPGPGDYELRKDLSVQTIQNKEKIIHRSISNKKLQNDDSFIHRLKNHNDCYGEYQLNAQRKLPELISKQQNKHSFTTSERSNCFLLPAISKGPQYYDVQDIKSDRSLNIGKYSSRKDIFDIQKDDISKNNCSKFDRSISIEQKMHKPKNHWSQDKPNRKYVVSYYQRVKPIPKFREYSYVSKDKIGPSLIDYPKDKIKGRTFKKDQRFKIAKINKVDIHQEYFIQKLVKNKDYVQSGEKDSSSSEEFINLKILDFTEKVFFDKQIRLKKPQQDVMRQKAPGPGNYDIKVQSGKQYSFPKGKRYNKFFWDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSR0</accession>
    <name>A0A8S1LSR0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0440001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSR0"/>
    <dbReference type="OMA" id="CHINQNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035925322" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="21743" checksum="1BB201D0C3436D21" modified="2022-10-12" version="1">MKKGLALIIIFFLLIQYTEQYCITQFKQQQSILKGNHKNVLQLQKGSLISSKKINFFEYGNNASFLKDNPYTIMTNGVDFTYCQLGKYISMTPMQKYLLNTLKIWLWEFDYIFQNQFRYYDIIIYAILNNEKTKIYENKQANCIVTIEFPDQFVEEFQVLNVGGNTINMELHIIKAEAYYKYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSV0</accession>
    <name>A0A8S1LSV0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0470009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSV0"/>
    <dbReference type="OMA" id="CKSVSEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="309" mass="36178" checksum="6C74DAE92160B77E" modified="2022-10-12" version="1">MLRSKENKEAEIYDCLSRDDSFIINDMENLGPWHKYGGSIHQELQRISSLQLCKSVSEHHTDKIINSTRQIAQMQSGRRLSQETIKQTQFPLDEKIMKSTSIHNESKSRTQLMKQLRSPKPKDSSLITTKQEETQTINIKAEKTNVGFFVQCYPNRNKFERSKLSNFQSSKEYFEKIALKMMYGLNRDQNIETIQIGNSNQQFIKQQEQQERTSQSRVGPQTVGRNYFYKKELKFHSNNTSQINLNKTINNSINSILNQQQNIAKQRIPFNKLINKKTVISFASQNQLLEKQYQQSQKQNKKIQRMVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LU09</accession>
    <name>A0A8S1LU09_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0430209</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LU09"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057496">
        <property type="entry name" value="FAN-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF123">
        <property type="entry name" value="PROTEIN FAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25400">
        <property type="entry name" value="PH_FAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="3">
        <location>
            <begin position="221"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="2">
        <location>
            <begin position="323"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="664"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8069123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="721" mass="84975" checksum="D5F3EBC4C3BFBF9B" modified="2022-10-12" version="1">MFFSLSSIRKPKQEFNLLHLETQEYYFLSLKVSLHNNNEIRGKLHICSRSLVFQPNQPKLPMIKMKYSNNLIMKILRNIDTKTMNQIMSIKWTPSLLMEDIDSLDINQLYQHFKRNKNNELLDRLYKIQSRTANQQYTIMFIRVDKLFVINRNPISPYITETCDENIVFSISQSNSKQNIVRFLKLYQGLTQAEDENSFINTIINTTLNEAMNEHRAQSSQQGLKIELAVKCKLIKPEGIIYGLFSIQRDEGIKYIPLINSPKGKILMWQLTDIKFFLKYTYMFKQIGLEIWFFNKKRSVLLVFEDIQQLETVYKYLIRKTTKNQISSITIEKLTELWVNGNLSNFDYLMSLNTFSSRSFCDLSAYPVFPWVISEYNDKTFDLSSPEFFRDLSRPVGALNKHRLQKYKQAYQDQLKNQKNNNNNDMIPYIYPTHYSSPGTVVYYLIRKIPEFVIKLQNGVFGPTDRIFRGIDSTWYTTLNLHADSKELIPEFYSLDPDFLINCDKLELGMTQEGEIIDDAIIPPWANSMTDFLLKMRMALESDYVSSQLPKWIDLIFGCKTRGDEAVKQDNLFYPYTYAENVNWNQCRTSIEKQALETQVAEFGQVPIQLFNSNHPNRKLKIYSTLRQQQQQQLNSSSQRNLQINDIQQDSQNKDYVTCLQNQVSSLQWENEKLKFNLDTIKQELQFQKIETFNSQYDQEEIDKKGQDLMSDDSLYQQIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUH5</accession>
    <name>A0A8S1LUH5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial import receptor subunit TOM40</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0430288</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUH5"/>
    <dbReference type="OMA" id="GRYTANK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07305">
        <property type="entry name" value="Porin3_Tom40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037930">
        <property type="entry name" value="Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10802">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8069281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="38155" checksum="B8427F8B76023289" modified="2022-10-12" version="1">MAAINKEQVDMDNPAFAQQDPHLKINDDEEMGLPPQFRNRYPRRPPLFDGLLNNVRAVTKKVEHIKGFKFEVAGGLSNNFHLAHSWMIPPSSKGKAPNPNPMKQPPVPSYTLAAQYLGGSLKTPFDQPTYIMTGRWDSTGKLEAAIIKKLNEMFNFRFSAFYLNSDPLNAQMHLDCDITGEDYVHSIKIGTGLYSFNMMQTIGNRLVLGYEMMTLTERNLSLMSYAMKFGINKKQNFYTQYVGAADQLILAYNHRLLDKAYFMSELEYSNQTGESRAILGYRQKFAISEVIVTVNSNLKFSSALTLQGFAYQLKLCAIADYKKDSYKFGYGIAMGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYC3</accession>
    <name>A0A8S1LYC3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Importin alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0510232</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYC3"/>
    <dbReference type="OMA" id="MIKEVQC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="466" mass="54550" checksum="8420F3380E1B62E8" modified="2022-10-12" version="1">MFNNPQEYAAQKKEAFRSEIRRMDRERIFNQKRILISQQNEMTEKDDLELTSLIPLIQQSQMNQNIQELKQLHLFAFNCVSGNNTNQIIQNQEFLITNNYLQHSIPSLMIKEVQCDVIRTLANSCNHKMFLQQQPQYLIQSLEISYQLLLTLTEFQDISDILIYLGNIANKWPEICQYLVKCLDRLLELSVSADSNLYRNICFCMHNICYVMDNQSLGSKDRITCLKILDQGVNRNDCDMLQNEILHPLTVLYAQDEEIDKYLISSKIVQYVFSTLKGEFEETTLQVLKQFSLTESKSFSNFLIEQDILDVALEYSNNRKKQIRKLSFLIVINLAYNDRQISNKIVKHKIINRIINGLIASVLQEKQNCIQVIQNLQKTGDNAVFQTLIELGTIEIIGNFIDEVDTVILKIFVDTLCRFLNYAKEVQQNKIIEDIKKIKPKLELIYNENKDPKFQDLFQLFLQLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M834</accession>
    <name>A0A8S1M834_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0550157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M834"/>
    <dbReference type="OMA" id="NKQMLSM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="16192" checksum="71FFCD3469EB716E" modified="2022-10-12" version="1">MEQVLNTLLTQSPTKQIQIVFINKNLLNRKNSLFQIQLNQLQKCQQSLEMQGEQQALKQSCIKRQQKSPLIRKQYTKDPQNKQMLSMTQQLHQKFIQQKQKELVQFLTICLTILSLIVMIVEYHQQENQRRFYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8X5</accession>
    <name>A0A8S1M8X5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0480009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8X5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="137" mass="16214" checksum="F459DF625FFE9488" modified="2022-10-12" version="1">MKTSFLNEFTLIMNLSNNLAEEIQGKIQAQQINFFHQKFIIILSRQKEKNESDITLEIIPNKQLSIVRQHSTTSSMKKRRRQINSKKQDRQISNGIYQQQQQQKSIENEIIDLIKIDELKLQNQFILQMIVIPIFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAQ9</accession>
    <name>A0A8S1MAQ9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAQ9"/>
    <dbReference type="OMA" id="MNICAIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="199" mass="23485" checksum="60CD6FB1BBEF1392" modified="2022-10-12" version="1">MNICAIRHIPYSNKNNRRQIVVDQNRAKSQQGSRLNSATHYRCSTQQSNNQTPNQGKLQFYEIQPYVGQLIKNQQQQFVSTQLTKSPSFRVKNYHPQDIIKSQTQQSSQNQLQSNIEKIERMNRMNFLGDLLQQMDGKNQYTRQSIITELATVPKNKIDIFNIKNQQEQNIEELHFYLVESQQRIKQHAFVIEQQKYPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBJ0</accession>
    <name>A0A8S1MBJ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0540217</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasmic vesicle membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
            <orientation evidence="1 6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="6">Cytoplasmic face of coated pits and vesicles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the clathrin light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBJ0"/>
    <dbReference type="OMA" id="PFEMPIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000996">
        <property type="entry name" value="Clathrin_L-chain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01086">
        <property type="entry name" value="Clathrin_lg_ch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8075461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="165" mass="19335" checksum="910437759CDEA18A" modified="2022-10-12" version="1">MDEFFDDPAGTIETYIVPEVAAQPLMFNDAARQPFEMPIAPNAGVTVLVSAEQQQRREKLREVEEERIKLIRERDQEERIQKQQRKEKAQQYLLQFQTQLKSDIQARKDANKQAQELRSLNKSQYKNSWDQIAQNVALKDGEYPGEKDVTKMRQAILNKRKDLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBY0</accession>
    <name>A0A8S1MBY0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0540085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBY0"/>
    <dbReference type="OMA" id="VNSIHRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000810">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362:SF309">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="273" mass="31489" checksum="11567F0782824D0C" modified="2022-10-12" version="1">MHIKTTLLSEGSSSNVFLTEDPNYVMKEFKSLYPASLRQKEAQILELLKGEHIVKLITYTDNYLILERLQSHDLFEVIKSQSLNHQLLKETCKTLIRIINSIHRMQVVHRDIKLENILIDKAGRIVLCDFGFAELLNSCSVKRTVGTLNYMPPELHQESLLGGMNSENINTQILIKSDVFSLGVTVFQIILGFQPFISTKPSANCKLWKLILQKKWSQYWVLVQKLSNIQIDPLTQNFLEQFIQPDTTSRASLDDIYSHPFLKEVKDDVHLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDB7</accession>
    <name>A0A8S1MDB7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0590258</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDB7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018724">
        <property type="entry name" value="2OG-Fe_dioxygenase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10014">
        <property type="entry name" value="2OG-Fe_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8078287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="234" mass="27535" checksum="F9273B64C21A0881" modified="2022-10-12" version="1">MKVVQALKSTKFPFASFTNRDVKSDFQFLKHHFENLVPDEYLQKRLKLPSDKIMRKRRMMNYHIEFDKDGNMTRFHEEYRLDNYDESKCLQHANIYEELTKEMKQDQIYKDFLYNTLQITWQLKQFQKAQISVHAVRTISYIDQPASPTPEGPNRDGNDLEIIGVVNKVNCQGAESQIYSSDKSKLLFEMSLEPGEGYIIDDKSLWNYATSHKPIDQSQLGIRDILGFVLKIDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ME66</accession>
    <name>A0A8S1ME66_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0550099</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ME66"/>
    <dbReference type="OMA" id="MYTYKEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF94">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="37"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8075835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="375" mass="44170" checksum="F7538DACDC0AF662" modified="2022-10-12" version="1">MQFSKQEAILKELFEDDYFTQIITHPSVQSPYEELTCAICYNLFDIPIILPDCKHTFCRDCLDQWVSKQAMNCPLCRIDVSHYLTNKQLKFSLIDFNTMFLIDNIEVKCPNCNWKGKTKDLKLHQQKYCNLMSCPLNCNLKFTSQELEFHKDICKYRIIKCPQYCGEEFEAHKIFNHLQNCMYTYKECNKCNCILQGIKMLNHNCNIDSCRNSQFGCKKQELHTNCDFEFNYCKNCYDFLMNKDLQTHTCQIQNTIQEVLKSYQNQLLESLQPVKHDLKLLENAMKKCQQESILLLDVPADKVNIVEILQQFGPIQNMKQYLNFPNLDQKKVAVRYFNVNSALCCKKYIKIFGITTQSLEIGQKQIKQLPKQKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEI2</accession>
    <name>A0A8S1MEI2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0600113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEI2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="63"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8078548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="421" mass="49144" checksum="2753064D1A831945" modified="2022-10-12" version="1">MKKNQVDQQVQSRIRNYIKYQAKIKQQSQNDEIEQILKQLPSYIFADLQKNIQTKIMSEISFYKSNFSKNVIPIISQTLQIQSYTPKEIIYQQGQLDDCSLYTVWKGEVLIVENSSGKLLATLAKGQSFGEIEFLTSQHRQFTAISKDLSQVLRMPRETFLKIIKYSKIDVEHFHKLKDQLILYQINQISSCYCCSENHSVNDCPYTFYKPNIDVIIQRENAQEVVSIRQTYERTGDKKGFFQLQTLKSNTDDKEEQGSRIHSIKNETAPAVSPSRPKTKTITQIHYKNYGQSNPKEDEKQIESPFFAQSQTEIQNEQNPNKLSIHQLLELPSIKNRQSQSNNQIVISSLSQLELIEQEFLKVFMLDIDKVGIFQSYFPQNNLEVILLSFRKYMRSKKVVITETLGDLTRRKIQTENNQFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MHU0</accession>
    <name>A0A8S1MHU0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0590115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dynein light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MHU0"/>
    <dbReference type="OMA" id="THESRRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21450">
        <property type="entry name" value="DLC-like_DYNLL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000018">
        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF35">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="1" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8078001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="101" mass="12107" checksum="FE8084DB8A2D4590" modified="2022-10-12" version="1">MSSQTTNQYRMYLGARVLWPPDCPDDILEGAINETQSCLKTFEAKDGQKMAEHLKKYLDTNFEPYWHVFFGKNFGCHSIHEKRRFIYFYIDKTAYLFYKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ML01</accession>
    <name>A0A8S1ML01_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0650042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ML01"/>
    <dbReference type="OMA" id="EITNIQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="44"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8081088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="162" mass="19256" checksum="73465B88FB4778AE" modified="2022-10-12" version="1">MMQENEEASSQNGSPNKISIQKKQKKKNEYDYHRKKRRGSDGISSKKSGQKFSQEEDKRILQLVFNNGPKFQKIHRHFHGKTLAMVKNRYYKYLRFRWEILGQNYKHLSVPQEQLETLCEQQKNVSNMLSAEKDDLISQITSRTTLLQNARMFVEYLVEQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLY4</accession>
    <name>A0A8S1MLY4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0590241</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLY4"/>
    <dbReference type="OMA" id="FPIQTFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07852">
        <property type="entry name" value="STKc_MAPK15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000238">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000166">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="17"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="352"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="357"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8078253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="547" mass="62425" checksum="DDB94B6CF314FA62" modified="2022-10-12" version="1">MENDKQSQFEDHILQRFTLLEFKGKGAYGVVWKAHDKQTKSIVALKKVFDAFHNKTDSQRTFREVIFLEQVSKNANIIKLLQVIKAQNNRDLYMVFEYMETDLHKVIRANILEPIHKKYVVYQLLKAIKFLHSGDIIHRDLKPANLLINADCLVKLADFGLARTVNSTSDDDVPILTEYVATRWYRAPEILLGSQKYSKAVDMWSIGCILGEMILGKAIFPGTSTMNQVEMILEILGTPTEEDIKSIAAPLAKPILDDFQYVKPKNFKTVFSQESDDTLSFLKNLLVFNPQKRLTVEQALAHPYMAEFAGSEDETVIDFPIQTFMDDNVQYSIEEYMTALYQHINQKNTQQVQQQNWFSSHQSPANASKPSNTLQQSTTPTSALDKRKKKGDSIEGCNNHSENKQIIKQEVKKKVSEDNQNQKMSAFEKFKSIENMIKMQKAKLGTSNGQITVQNSSKLQYGNKSVNITRADSLKKKEASCELLVPSPAAKSKIQKIVSQNSFKDPQKIQIIKSQSKLPKHESQHDKFLNKKTYLQYILSGKAKLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNN6</accession>
    <name>A0A8S1MNN6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0680085</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNN6"/>
    <dbReference type="OMA" id="LIIQHQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="21"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8082667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="575" mass="68005" checksum="62BE9364F6E4C8EA" modified="2022-10-12" version="1">MKGIQLQNQLKKDKKMNIKSFYIVGLIGEGAYSEVYEAIYLKNNRKVAIKKVYKESITLSNKQSEIYIERHMMKQYSQKYPSVVEFIGSFQDKQYLYFVSEHCPFGDLGNIIYDIYQIYRNTRSQELEYFIKVYIYQITQAIIYLHNEGIAHLDIKPKNILIDKNYNLKFTDFATCFFFEENKQPPDLIDQIKKIQQSHVRSIKKIESESTQYRSNFVGTPEYISPEMLNNSRASKEADLWALGCIVYEFYHGKQPFTDISENAIFNNILTLNYELDNSIPQSAQDLIRSLLVLNPSERLGQEDTQLIIQHQYFQDIKQDYIPWQTEIQIPFEYKDLFQEIIPSQSKSSILWEFTPANVQMKQELVKKKQDRLKTIMEELDETVTPIPNRLIRPQKIHCSPNQPIFKPERTIHQSPSNQDMESHCNSVFKHFDKCVGFIYLNTNRWFCIPQIAILFGYLKPPCLLIDFIKGEKKYLPIDDTIKISNDGVHYVHTLEIIRCQNHPRFFINLKMLKQSLQIGSRQFNQQKMNILINDVLFFIFPVHKLFFMNNNKISIQFMNELQKIIQIVKQLNPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNY3</accession>
    <name>A0A8S1MNY3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0630113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNY3"/>
    <dbReference type="OMA" id="IKAVNNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08877">
        <property type="entry name" value="START_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34560">
        <property type="entry name" value="POLYKETIDE CYCLASE/DEHYDRASE/LIPID TRANSPORT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34560:SF1">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="52632" checksum="DF9EF3A3F33D3B10" modified="2022-10-12" version="1">MQNKNKIFTDNFQCNFFDILSYKELKSDLEKLMQDINEYYQNGQDFHEAYQIYVFVREKLMCLQGFNKEQITDLIQQLDKNEEMIKFYDFYREQHSPPKPQQLKNLLTHFQREQIKHQEQQFPQIHNNQTIIVVSAQEINFQQFENQQILSEIQLGPSCQELYNIVLSCDNDSKLDKAYQTIQLIDKTQLSQDQIIRLSVIESSYEFMTKNLIELDSTGWTLDKNSHGISISYKFPPKSSTVSLLMEAEIEADCAKLMSLITEVELFSEYVPFCNYATTIKTLSKTQKVCMSQLYFPVISNRETVFLGQGIDRLEEDGTILFLCKSIDQDQQFLDYYDLQLHKSKNVRLQLNYYIFQITPITKTRCKIKAVNNCNPQLSFVPTWLVALIARKFAFQLIEKIVKYTKSFEKYPWYKKTQENPEFYQWLQNKIDNYFAQSINTKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQ77</accession>
    <name>A0A8S1MQ77_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0670200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQ77"/>
    <dbReference type="OMA" id="IMNACKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="386" mass="43799" checksum="EF5D3F1D700F49EA" modified="2022-10-12" version="1">MNYFPCPIHNGHVLSIICTDKSCNDTILICPLCEHADHQNHQTLPLKIMLQLVENSHNSTNEQNYNSIFDVSLFLDRSHNECLRTVQRFNEALENTSKQLIKKVDTYYLDLKNQLKAFANSKIPIHYLLDSVQSYTDFQQYKKNIQQILGQFAFNNGIASIQSIDAAIMNACKIQIGQLSDQLIDLQNEFDGKVEIMGNLFRAQPITAVDLVQSISFQFSPQYKGANITISGQNMIAEQKTSESNGQRFTICEPCIPKNGVYKFGFKVIKYAGWIGVGVCHREVIVNANYKFNYTNIGHGSYLISNNAYSWSHLQKDLNSAHKSFDFGVGDIIIIEVDIPKKKITWQKKKTQIKFTMNLDTSQDLYPCANLCSPGDTVEIINKIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWC7</accession>
    <name>A0A8S1MWC7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Cytidine deaminase</fullName>
            <ecNumber evidence="4 13">3.5.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 13">Cytidine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0720220</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="2 13">This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2'-deoxycytidine + H2O + H(+) = 2'-deoxyuridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13433"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15698"/>
            <dbReference type="ChEBI" id="CHEBI:16450"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>cytidine + H2O + H(+) = uridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16069"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.5" evidence="4 13"/>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWC7"/>
    <dbReference type="OMA" id="LTHFTCV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055086">
        <property type="term" value="P:nucleobase-containing small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072527">
        <property type="term" value="P:pyrimidine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01283">
        <property type="entry name" value="cytidine_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000008">
        <property type="entry name" value="Cytidine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050202">
        <property type="entry name" value="Cyt/Deoxycyt_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006262">
        <property type="entry name" value="Cyt_deam_tetra"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01354">
        <property type="entry name" value="cyt_deam_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004064">
        <property type="entry name" value="PRK05578.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644:SF2">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="14">
        <location>
            <begin position="3"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="44"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8084708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="144" mass="16066" checksum="95E1689F8B0A8B73" modified="2022-10-12" version="1">MEITEEQLIQEAIKAKERAYCPYSNFRVGCSLLTKNNKLYTGCNVENASYGLCVCAERVAICKAVSEGDRQISTIVVSCDTQEPTFPCGMCRQTIIEFCYSGNDIKIIAIGKDHSKPKYSKGSEVIPYAFVPQDLHVDPQLEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWF0</accession>
    <name>A0A8S1MWF0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tubulin--tyrosine ligase-like protein 12 SET-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWF0"/>
    <dbReference type="OMA" id="QKGDECT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057954">
        <property type="entry name" value="SET_TTL12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027749">
        <property type="entry name" value="TTLL12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46088">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46088:SF1">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25556">
        <property type="entry name" value="SET_TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin--tyrosine ligase-like protein 12 SET-like" evidence="1">
        <location>
            <begin position="331"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="799" mass="94095" checksum="7A26014809850C1C" modified="2022-10-12" version="1">MNFQNLTGELEVFYNNHGVQLAGINFPPKVELLEQLKTKLENKIYDAGEYFKIVDDQEQESYVLQCTKPLKAFEKVFLIDHALSFRYTELHTAIKQVDRLKNILKYWNKKQPLFKIDKVYYHTEDFDEMALETLKDVKPKEDTLAISFFGNNINSIDEVIHFLNNYKQIKAIWLNGNPVAENKEQLLLSIEEQCPQIELVDLSFTSNATEWVIRYVTADFDTKKANSQEPLNYLNLSGRNVLRLKSVKQISNWFPTLRVLDIKNTKLNDLEEVNAFFSLLPNVEYLTCDLEVEDVLYELHKTNKLNAVAPHLRLVNQRDIRYESYTPAKEEHNDIQLIKQNLFKLTGNYRLLTSDQMDESAVWYLMDELGSSIQHSDTPNVSVVPFLYLPNGKIDDDAVAYSILFPIKDIHEQPIFRDYLPGVDEAKFRSYRLNVWFDIPYDFILNGYNEYKKKLHNLKIVEPQIISKPIEYLPKENIKVITDLEMVTKNLTLPYVSFTLDIDEANLVFFSAGVHDDINQKYADKFINQYPHEYAIISKEKLANTIQSTYGEVPWLQETFNLQTQLPLFMGAYKTRELQHLNNLWIIKPPNMARSMDMVVTNNLDVVLRLIETGPKLAQKYIERPLKLRNRKFDLRFIVALKSIEPLSVYLYKTFWIRISNNPYTEDVNTLSIYETHFTVMNYGHKLTQIFMHEFIEEFNKEYYPHTWEKQYESIQHMVRQLFIALRTHQPDMANYTKNRSVYGMDLMIDSNGFQPKLLEMTFSPDCTRACKYTPTFYNDLFECLFFGVENQNMIQIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZS3</accession>
    <name>A0A8S1MZS3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0730016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8084842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZS3"/>
    <dbReference type="OMA" id="HNINEMA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="237" mass="27802" checksum="BC5FF1738CAF1E98" modified="2022-10-12" version="1">MNSTTPKSSGINYAKLRKQRSNQDTLPKSMSTNIKMDINQTQQFGGIKHRSTSQHNINEMAQIQLFDQCLEKQANQNEFLVFLDRQMQYFCQNTPQNYSSFRITNKAVQEEKKKLQVELQKCNQENAYLIKEIKDLNKTKQMLVKRYDALQGLILKERLNNQPKNRQFSNQKFQHLFNQISPVQETISSLTDERFESNTTSLDSSLSYEDDQSSASQLLDLLQKNSKHRFNFQKQMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2C7</accession>
    <name>A0A8S1N2C7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0650062</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2C7"/>
    <dbReference type="OMA" id="RIVHHSG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004977">
        <property type="entry name" value="Ribosomal_eS25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12850">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03297">
        <property type="entry name" value="Ribosomal_S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8081124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="139" mass="15116" checksum="A97503F7AAC2DAE8" modified="2022-10-12" version="1">MGKQPTQGQKKTKDQIAKAASQRSKQTKKKWTKGRAKDKLNNAVYLDQSSYKQIETQLPKMGSLITVSTVSDKFKVNGSLARRCIRHFAKSGLLVPAGDQNSKQYIFTVNAQVVAAQKAAAAAAAADKPQTQKKAPAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N3X5</accession>
    <name>A0A8S1N3X5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0770043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N3X5"/>
    <dbReference type="OMA" id="EAFVFWP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="29574" checksum="C6AF405933292159" modified="2022-10-12" version="1">MEDLDYAGINFGVIAFLYLSSIFLILKTNTKSFNKKIQDQKETITNSSVEQILEAFVFWPCETKKNHKLICLEKVIYIFVIFTNQEVAQSLYEQQNNIQDTMQDYIYKYLYIPIICFGGLVFQKVFYYTFYCFYEECIFKQLEKKYPKTVKFAFSDPRSKSMYYFGYVIANLVVILIVFIDSKDDIFGNCIVSAVISSLLQIFGLELLFVIVFVYFIQIDNKKYTILFQQKKLISLIDKTEKNNNESTPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4G9</accession>
    <name>A0A8S1N4G9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0720174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4G9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8084616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="132" mass="15226" checksum="DA44FF1EDAA58712" modified="2022-10-12" version="1">MKILDEIKKQVEMEMQKIKKVQPTRIHQPLNSQLKKDLIILINKGVQLKDAARELKITYHEAKIAYNEYRRKALSSQSETESTILDIRTAGVSLLRVPPHHFIVQSVVENTITSVRKLYNVIVQNPILNSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6T5</accession>
    <name>A0A8S1N6T5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">LMBR1-like motif protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0740216</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LIMR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6T5"/>
    <dbReference type="OMA" id="QLERICY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051584">
        <property type="entry name" value="GPCR-associated_LMBR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355:SF0">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="479"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="556"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="566"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8085596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="582" mass="67984" checksum="33EFF4B0F033CC87" modified="2022-10-12" version="1">MLTEDVVFYSIATTILFIYVVYLIWEYSAKDVPLYIKILTFISWILTFSIVLVLPIDIANSTKQHNEAQQLETMGNIKILWRIMYWGNFFLAWLVLPFFQDYEGSGDFDIRGKIRYSLKKNLTIYGLGTVIIVGLTIYLLIKDNFDSTHVEGVLIGVSNFFGLLLVVILLGQGLVAIPKLYYREHKEEEVLEQCYQQAVLLDEQRTEKTYELEDICRTLQLLQNEYSDSEFSRYLMIILQKIPQEFQKSIRQTLQKYNASNIPDKYKPLNLQVLASIHKYVKCLMFDLQRIQTKLNIVNQKALKFERKDLADDETQFETKWSKMIYKISIIWNLKLRKYYCVVLAITYALLSLFILFCELITFVAKTNKELNPYYLLLSSINRYYLTELVLLLPLLYMMFCTYYGLFAMKISGLISFNKHHHTDAPSLMFGSINFARVSFPLCFNFIQITDILEDQATSFSETVGNLAESLFVQSYKTILPLMLLIMCFFNLFNIGDKLMRTIGLGQYAQQERISGMSLEGKKIIEKERELKKKNNLNDSNSQIVFQELQIMPKSKIQDEEETSRKNSSNQGIRNFDQILSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7Q0</accession>
    <name>A0A8S1N7Q0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexose transporter 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0810203</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-fructose(out) = D-fructose(in)</text>
            <dbReference type="Rhea" id="RHEA:60372"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:60373"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>D-galactose(in) = D-galactose(out)</text>
            <dbReference type="Rhea" id="RHEA:34915"/>
            <dbReference type="ChEBI" id="CHEBI:4139"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="7">
            <dbReference type="Rhea" id="RHEA:34917"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glucosamine(out) = D-glucosamine(in)</text>
            <dbReference type="Rhea" id="RHEA:78423"/>
            <dbReference type="ChEBI" id="CHEBI:58723"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:78424"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-glucose(out) = D-glucose(in)</text>
            <dbReference type="Rhea" id="RHEA:60376"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:60377"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-mannose(out) = D-mannose(in)</text>
            <dbReference type="Rhea" id="RHEA:78391"/>
            <dbReference type="ChEBI" id="CHEBI:4208"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:78392"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-xylose(out) = D-xylose(in)</text>
            <dbReference type="Rhea" id="RHEA:78427"/>
            <dbReference type="ChEBI" id="CHEBI:53455"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:78428"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7Q0"/>
    <dbReference type="OMA" id="VAQFLCM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="265"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="362"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="398"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="16">
        <location>
            <begin position="14"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8088600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="475" mass="53565" checksum="A6E269A17D7CEB82" modified="2022-10-12" version="1">MDIQYKSALPFVLLVFNAVLGSLFFGYSISYLNSASNFYPIYYLIIDYDVSTQNLLKALVGAIFTLSAGAGAMLSGNLLQKFSIRKSFMITDLIGIIGVLLEIISNIYWFYLARILIGFCVGLNSSLIPLYIKEFSPNRLSGSLGALNQLTINIGILIGLISAFFYKSNVDEQSNDNIFRFFLEIPLIFCVIRTLLLIFVFKNEPPSYYVQQNQLSQAQQIVEKYYLREKVDQVLDKIKSVIESKRLQKETYKDLFMNYSQRKRLLIGCGLQVLQQFSGINAIMFFSKIIFSSILNNDETKINWANVTVGLINITFSLLAINLLQKFGRRTLLLYGTITCTIFLAVAFGFSFSQDPNTTIEILIVLAIFGYLGAFQLSLGPVAWIYDADILSEKGMSLAVLCNWISCTFIAFAFVILDIDHNKERLSIVFGVFTGCCILGVFFIFKFVQETKDRTSSAINIFFSLTKVKEEGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCC2</accession>
    <name>A0A8S1NCC2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0820130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCC2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="108" mass="12870" checksum="477B092983A1A0ED" modified="2022-10-12" version="1">MIKIIIIKNSIKSQQFDHQTQKIQKKSVQIVFQNYESNIYFEVIFYGCGLNEENINSKRLDDCKLGLAQQKYIKLLGANDKQIEIICSQFLKQTRILFELKIFYCRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NH74</accession>
    <name>A0A8S1NH74_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0890216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8092088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NH74"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="124" mass="14749" checksum="C077CF95EC488926" modified="2022-10-12" version="1">MKKKIQSFKIMNKFVYIILGCLVLTLLTQFFQLNYRVFMANLCLMSTTKLLKLNFKCIINYQMKCNILLYHDGIRSASKVIIKYSLIKQLNIPQSYENIANCINNEYMKENPKIRIQESSHYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NI50</accession>
    <name>A0A8S1NI50_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidohydrolase 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0890063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NI50"/>
    <dbReference type="OMA" id="HEPQMDE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22642">
        <property type="entry name" value="IMIDAZOLONEPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22642:SF2">
        <property type="entry name" value="PROTEIN LONG AFTER FAR-RED 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase 3" evidence="1">
        <location>
            <begin position="83"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="560" mass="64656" checksum="C7711AA8CA4833C2" modified="2022-10-12" version="1">MNNQIQQLDIIVIGTIYISFQPLVQVECMQIKAGKISRMGQIDEILNQAQMQNENLQEYMKKSGYWELEIDNMRIIKLNELQMAMPGMIDSHVHPQSGGIQLSRLKIQKYCSWEDAKNAIKDSIMKGEHDKNEWVFAYGYSQGWFKEIKQNVQELDDICPEKPLIIMRDDCHAYWCNSKIFEITQSHQENGIYIEEQMNIVRKFIPKLSETEQQNCLLKGLQHLIKYGVVAFQDAAVKEKLFHNYIKLYQNQNYSNQLPYCCMSLCWNDLFLEQTQSIDEEVRFETVENQIKQIRDLNLQKLYCHSVKIFVDGVLESKTALMNVQYCKCQHNGQLMIQNIPQIIEGLNDRGIQAHIHSIGDRATDIVVEAYERISHKFNKNVINYLAHLEFVSPKAIEAFKKFKLGANFSALWFYESPELSLMPTCLPEHYMEGMYPIRDLEQCIIGLGSDWPVSSANPFDAIEVAVTRQPLGHTQKPIPIGDLKQRITILQAMHYYTEGSAQLIQKPGFGILKEGGPANFIIISQNVLKVKPSEIHQTEVLSTFIDGIEVYQTLKVDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPT5</accession>
    <name>A0A8S1NPT5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein-serine/threonine phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0820023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPT5"/>
    <dbReference type="OMA" id="MKLVDMC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033192">
        <property type="term" value="F:calmodulin-dependent protein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097720">
        <property type="term" value="P:calcineurin-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043360">
        <property type="entry name" value="PP2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45673">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12508" checksum="2E3A59DCBA8DB314" modified="2022-10-12" version="1">MDLLKDPKGDRQVNTIPTPPHRPLSEELLFIDDKPNWKLLKEHLFKEGRITKSQLMKLVDMCNYHLKNEGNVIYVDDPLTVVGDIHGQYYDLMKVLEMGGDPEQGKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NR76</accession>
    <name>A0A8S1NR76_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0910082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NR76"/>
    <dbReference type="OMA" id="RIEDYEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="458"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8092696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="522" mass="61584" checksum="6846D491774B71F4" modified="2022-10-12" version="1">MKADSFHSLFSDKSAKYNQFISSQLFVGYKVRGKDQQKKQYIVSSPSDDQKMSNNFTASQSYVNAMKALQDKIKQLELENQSLQNLSSNDSKSNLKSNERKLKESSVHMKSSENLQIKELEQKFMQQEFISKMRIEDYECKITQLQQNLLSITQQADQKYREYMEEIQNLTQQLRIQEENNNNYRNKVNSQNQIIQQEKQNYSNIQFLLEQEKNEKRFLQQNNEKLQYELSKLKEKLFEADTYIEQYINCYNDSKFRKLEEENNQLKLQLDHLTTENQQLNESIQILKSNLNLKTKELEESEFRRVKKSSESNIRIEQLRQQLITLSTKSNLTTISPRITKKKNIKQVSSKEMSVKPSKIRDLRQIKSKSLNKLMDPQFLTQSQSMQNNNFMHQKQQGYDSQSSGVSRLIVKTLEENYPSSQKNEICNNAVIIGNEITQTDQQSGRKNQKYIEAVQRIRFLDELLTALNLQYQDIEEQIQQQTDMTIKKEKRQKLLEIIDSISDVNKELNDLVTIKKLASQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTD2</accession>
    <name>A0A8S1NTD2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0960159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTD2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="84" mass="9983" checksum="83510CA9A28CED4A" modified="2022-10-12" version="1">MDQRKKIRRKQERKLIRQGNFVSKKVYIIDKCCFKGLKGSEIFVKTGTLLELCKNLKRLTINSKKNNVNKQCFFESIDSPDEQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1G2</accession>
    <name>A0A8S1P1G2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0950121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1G2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="29207" checksum="1F2A982FB2D59F41" modified="2022-10-12" version="1">MKFIVILVDILQNFMKNQNRKGKPKLGQVCSLILFKSCSYIQKVQEIVISLLFNFSFFKRLVRCNAIKSGVKEIGNMFEKPSLNSCLSQLKKDYCIFQKGQEVMRKFFLFLKEISNISECKTKWSYNLNLQFHYSIAHANKYQYIQVFHNKAFEGFLKIFQFHSFVKYSYQLKFNQKLLFFSFHYSFKLTISLMLLFYLFIIYIIIIVQMNQYSFLNNQTHQKYFYMNQFWKKQFQINSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P217</accession>
    <name>A0A8S1P217_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1030075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P217"/>
    <dbReference type="OMA" id="QPNIQFG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8097143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="840" mass="99675" checksum="B9D97B8E2F302952" modified="2022-10-12" version="1">MKSKDNYKLLGLTVFLAATSYYYLQKRKNRPLCQKIADKFQSSYDKIESYFYGKGIEELKSSLVNNQEYMQILQQAENDFFDLQIVYEEISKVDFLRELSILNKQNNQLLEIKLVAQYVEKNSLRLVQQQIIEKLASQRMTIMQPNIQFGDSTIKFLMFVQCAFLRLIYEVENVLKIKNNNHQIIRHNFILKCILDYLNKKLTDYPNLKNLVAIVMLEKIGSNQFCFEFYHDCKDLYYTKTIEDCQCLQSCDCCIFDLLTRYISIFQLSFQKQLFEFIKCNQLFQTHEHILQMGKILLKNIFYLGGQLNQNQLFYYDTFSPLLNLLIEATSNKNIQEEILKSEEFRVSIQYIEKVIKSNENSFLNNFLDGLIDLYAIIYPLFYNQNALKSVINDDVKIKSILSTFFGVLLNLESRDTYTSNKQNDISILDWFAMNMGSIFTQQLLGNISVEFILQLLKLKEIQKEIIDTYIYCLRTSQNKRIQDKLQQKQNIDVNTFISKLISLVVISLLKEDFTNQNMDSIIDELEFVDEDERSNIFLNIMRIQFEIPSMNLNYLRYVSQKCLQLSEENFQSFNGYFTNLLNPTTLSYDQYMISMQIGIIYGKVDSFFASVGQSFQRDLQVYEQYKDFLQRDLILIVDLMENPYYFANVLVHNGNCPEDIYQNLIKKAAISIWQLNPKINFSQLQYQISLFLCLENLPKYIQEMFIENQNKIIRLKEQYEQLWPFQCLQRDLSSIDIYLKLVGDEVVFNELLQDQASFEFLSRIQEVLFKSPNFIQIIYQLLLCVNRLNLKIWIGKILFYAKKLRLNNKECDILLKSKECQSFIQEMPDNYRKYMLLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5S4</accession>
    <name>A0A8S1P5S4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1070006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5S4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035896738" evidence="2">
        <location>
            <begin position="18"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8098368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="346" mass="38942" checksum="E827D6595525AA72" modified="2022-10-12" version="1">MKYMIVVLFALAATTYASKTQDQILALLQTGTKASDAIDTVFGLLNDLKQSNIDAQFAADQKNETDEWIGAQTIEQFTKIKALNQKLFQQAVENRAEYEQVLQQTKNYLAWNEARRDSIAAKIETLQDNQCFSNQLFVKSIKHNQEALEVIKLLKQDVAGYIINGDSFEFTQVKAQSVAEKLKQYSNLFQEHQIKSFLALAQEQQEQSSGNGSTLAEKVLAVLEGLESELEASLENLKQNEINASWELAGWVSLSEAEITSLEVEYERKQVFADRTATQIQAALAQQAKSKIILQESQDALDQAQADLEAKRADYEEAKAKRNEENAILDEVIIMFKKQVASWSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7C7</accession>
    <name>A0A8S1P7C7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1080119</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8099002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7C7"/>
    <dbReference type="OMA" id="DIFNEAP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF26">
        <property type="entry name" value="MFS-TYPE TRANSPORTER SLC18B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8099002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="22366" checksum="79C01CDAFA82B55F" modified="2022-10-12" version="1">MCLAYSYFQPIFAIFFQDQFDVSPVDSGYYMSLAPFSYCLGAFLISRIKVHKQITILIGIGVVAISEFMMGPDPLFGIRPQLHLTMIAYLIFGFFIALPYVLTLPSIIETLEFRFEKKDHEKCICLASGLFNSILSIGEFLGPFLSGLITEYLEFPRSCSILGIYLILSTVLYIPNVFQNKYKIQRVKVQTFDEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8Y1</accession>
    <name>A0A8S1P8Y1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1100045</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8Y1"/>
    <dbReference type="OMA" id="ECFVRNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="4">
        <location>
            <begin position="134"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8099596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="257" mass="30368" checksum="6E348CC0B51B512D" modified="2022-10-12" version="1">MNQYSQKILEKRESFAEIDEQVNLVYGDQVENVIEKSQQPQLKYMMSSSSSNDKDEPIQNSSLKEFHLNSIELKVNNQFQNDIEIQSYKHTNDSVQDFNYNEQNKPMRTRRKLNVYKSLIDFQSENNSSSQNQSIVICKCTKSNCLKLYCKCFHQNKKCNDLCNCIECKNKEEDFGIRFNALEKIKQKLHKQNNDDDLFDISKIWGCNCQKSQCQKNYCECFVRNQKCSSICRCKNCANKKKFPFQQQKKQKIQTQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P9Y1</accession>
    <name>A0A8S1P9Y1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acid phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1110067</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8099976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P9Y1"/>
    <dbReference type="OMA" id="MLYLTEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00839">
        <property type="entry name" value="MPP_PAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041792">
        <property type="entry name" value="MPP_PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025733">
        <property type="entry name" value="PAPs_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45867">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45867:SF10">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14008">
        <property type="entry name" value="Metallophos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035866757" description="Acid phosphatase" evidence="2">
        <location>
            <begin position="20"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="3">
        <location>
            <begin position="133"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase C-terminal" evidence="4">
        <location>
            <begin position="369"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8099976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="57829" checksum="2805BA5F34D89607" modified="2022-10-12" version="1">MFMIIFLFVMESIQEQCEAYGVRLFLGHYYTSLKIDTDALRIVFNTKNICNNQFQLFIKGDLEFNITNYKTKLLNMTDVYIGTKANIEYETYIHTFQIPKQLSSIVFYTLISNDTIIKQAQVKLPHLQQETTKVLFFGDMDSSWVYNRSKQTFDWFENQIKQKIQFDSLIFTGDMAYDLESDNCQRGELWLSRISLFTSQYPFMITPGNHDGGFDYKYTFLREHFQMLYLTEYDTQNYQNDFYSFNIGLVHFIQYDPVMIVYKADPNNYTKERLLSQFRNDLITAVQNREEVPWIVVFTHYPIYCNFMDDDQCVNNFKYLAEFEKLFKEFHVDLYVSGHQHNYQRNYPYYQNNSASFEMDGNIYYNYESPITIIEGAGGADYGPEIMIYRDQPYTAKQLAQNGVGLLQVMNKTHLQFQHIQVSTNEVMDEIWIIKTSDINNEKDNSLKYWVISIIISAIVILVLFIFIKYNVRKQKTYQKAYDSNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PA72</accession>
    <name>A0A8S1PA72_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1100066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ANIYNRS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1349"/>
            <end position="1371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1426" mass="167377" checksum="3AA1B64DE9B2F9C0" modified="2022-10-12" version="1">MYIIIYFLFHEIYCQCYQKYKDQTIYLSPGETLKLDLQNYIYGKQLQYSSSNNCLESKIKYKNITLNNSQQSDYSEYSLCYDPKSGVQLGQTIQMYVVNEKVIISFYNISESFSIPFYTTEQIIDTNMICYNVLCMRLNLGLIECNHKIKKQSIIFLANATSQQTQKIFQQTNELGSIQAGPINYINDDIVNYYKMIYVQDESRIDIYTINITSKDVTVSTRIDKQIYQLQSLNLYDFLIYNSALYFLSKELGLCYIKNYINQYCWKMTDIFDVFSVYIEQNGYHSILLGSTITLDIFKVNLFDDQTYKIIQMYKLQKNMTFSKIFHNDKYSVIVGNKNNNTKKYFLEIYSQMDDLTSNLYKTIEITSSKELFYFDSLNNNAYAINNKSIFIITPQVSYITIDDDQCYGDIIEIQASFPSLEIESLCNFTFYIKKLEQESQDIYDRQELDSSVIVLNKSINNLYLNNLVIGPNVDYYLYNKSIDNYFKNSIKIQNKNSNEDDFERIKFKEQFRISYAQTMDIYSIIFNRAFRIAIENQYLQIIQWKDLTLQIFHCIIYQSDQDINCNYKQSIKFQQPILQVVTGLICSSECFVIKQEKRADLYIEYNNKFIVTNCFIESIYNIQTIKLHLEKYLVELENNNIQIFLPVYKQSNNCNLEKVFEINSLELEKLINTNKTINIFNVKTNINIYNLYISTNVGLIIIDPGLLRFQQVQLIAFVKFKQQFDTVLQVIRNRIITLQYQSIMIINLIQQNFYYTEKLLPKYDFTITNTTNSQVAFSQNYFFLQAKDSNYSDVIIVYKLDEPQISAFHTYFLKTNNLDFNIIQNQEDEVFLVQLFDAQKLYSSVILQFKSSQSKIIKVLFQGFTSYAKLMDVYVNVKTSEKIYNLSLIINETQTITYNNSDQDIKKLDGYNDYIDGSVSEWSIKCQSCQKELELINNINYNQYLTSIPNTTQIKHTNDYVLIQQEQSIIAIDNFGLVKFYIPLPLSEQSIKCTSITANTWETKTKLVVSVCNTTSSAQFYITSFLSSLPVAMGPFYSPLVINQITHIRMLHEILFILDVITEYVYMFNLTIEDNQTDNLRVINQLNAREFTADKAQVFVSFDIAYNDDTYLLFLLTNSSQFIIYTYPQDDAYTSDLISFFYELGFTDIPSNIVPQSLIVAQQGQKQEKEFYRIIVANQNFHHYELEITIEYLQKDTLINIEFIRAYMQYGYFYATGKIRVSEETQGFFGLIYQNPLDEKQKLLVVYDRLSKSKEKLRKILGGYKIFSNNQILFDFLHLKKKNDEFSFGIILQVGSQLSSLNLSRYLAIKIKDKEFQTQNITFNASNDFGKWVSFQANIYNRSGQTNLLLIMLSLGIVVFIVISSSLLYIQNKLNKLELKDLDVIEEEEVIVKEAIAIEEPISIEEKEEYDIYKEIEYDIVKDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PAL4</accession>
    <name>A0A8S1PAL4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1100123</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PAL4"/>
    <dbReference type="OMA" id="KWAREIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000019">
        <property type="entry name" value="Oxysterol binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF148">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="130"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="506"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8099753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="61297" checksum="E6FA49EEF7737C9F" modified="2022-10-12" version="1">MICCSSSKKKDENKKPIEVSEQLIPDNHQGDRQTDQSCRTNQQQEVELEIEERPKKNSVGYQVQDENVSASICPSFVVGKTPFTLFVISLLEKQQNMKKESQQQEEIKIDFQEDLSVIVSYPQIEAEVKNNEKDKSQLEEIPDQPEVIQEEDEEEEDREMSEEEAKNAAIYKWAKEIHDYNTDNTEELILKAVSNFKPLQSKEESRSTLAHKDGGKVCKNKKLIRTLRAIGKELIKQIGKQILSGNLNLTTVSFPIKAMIPKSALEKVLMSSCLFPLYINRAVLEKDPIERMKLIICATLGNFYLNCSFLKPLNPILGETISGFYQDGTTAYVEQISHHPPISYFLTIGPNNSYKFYGYYNYEAKAGVNSLSLKNKGKRFLRFADGELIEYNFAGEEYSGSFWGPMKVECKGKITFLDKKNDISACVELDSVRWKASDYLSGEIKNKGKKVCKIYGSYMGYIEFGEIRYWDVNYIRPYECKVFKPNQFLLSDASFRPDLRQLKRNKVQEAQKEKDDLEMQQRQDAKLRKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBK4</accession>
    <name>A0A8S1PBK4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1120099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PBK4"/>
    <dbReference type="OMA" id="LYMYVIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8100405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="37489" checksum="4C75B873A291CD90" modified="2022-10-12" version="1">MIKPIYREYQNHQGDNEDPNNTKMVQITDDSSLNRINDTLKDSINEYNKYGVQNIFSPSKNINDKKELKDDTSQDPKNIQLVIQNITESDKLIEINTTDHGDKEEISKFQKGMLSLQTIKYQKKRIQFRNPKYEEKVKTKKEFNWNSNAIKQWKINVNIVLFVLNLLRVIKHKKQEGNKITQSQDVERNQNIKSNFRLKQFGLTILKFIVGISLALFISVLIFPFILISDLFLRIYTYINLYVRYALSHENLFYKFILLIQLYMYVIAIVIAIITNLYQSIITTVELIYNIVGTLQNNISDLTRLLYQTIQNPFSKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PE70</accession>
    <name>A0A8S1PE70_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1160059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PE70"/>
    <dbReference type="OMA" id="FECEEMY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="389" mass="46595" checksum="B98E88E2450BB5E6" modified="2022-10-12" version="1">MLQRHSETIEYMRECFRQKILRDHREAIFKNKRRPYECKKQGILRNDSLNNQFNYLYLGETQNVNETQVNTSKKNEQEMTIQQQMHQILNVEFINNFKLINLETNLLISLNKFPALSNDEELTLWFQLIEQVIMQSDFQSIIVNISQLEQIINQAIKYKKFDTLFRTLNCLLKLVQLNDISILKYFECEEMYNYLIYALQNQQLNAIVICEKILEISQYGTLFLIRQRLLDYIYNFLLENFEFKNFFITFLSKINCLNVDEAVTTLIRSKIFNEIINLHGTEIDAQGYRCIGVLILNLIQKVNNKDQLKYLIYNTNLMCMLQQILSLYHNEDLILMGIQILQIIFNQQNYEFVQQMKKDIPQILLQLYFLKKCCVENSMSEKINQLIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PE75</accession>
    <name>A0A8S1PE75_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">alanine--glyoxylate transaminase</fullName>
            <ecNumber evidence="2">2.6.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1150081</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.44" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PE75"/>
    <dbReference type="OMA" id="MLVPTNG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047304">
        <property type="term" value="F:2-aminoethylphosphonate-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019700">
        <property type="term" value="P:organic phosphonate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01376">
        <property type="entry name" value="PhnW_aminotrans_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012703">
        <property type="entry name" value="NH2EtPonate_pyrv_transaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03301">
        <property type="entry name" value="PhnW-AepZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010006">
        <property type="entry name" value="PRK13479.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02326">
        <property type="entry name" value="transamin_PhnW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42778">
        <property type="entry name" value="2-AMINOETHYLPHOSPHONATE--PYRUVATE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42778:SF1">
        <property type="entry name" value="2-AMINOETHYLPHOSPHONATE--PYRUVATE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="6">
        <location>
            <begin position="26"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8101377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="365" mass="41323" checksum="508D4B7D7E764D53" modified="2022-10-12" version="1">MQILFTPGPLLTTKTVKEAALVDMGSRDPKFISIIQNIRSTLLKLSNVDEKDYTAVLLQGSGTYAIEATFHTVLKRETDKVLVVSNGAYGERQAKILTAINKQFVKLTYDENQKCVVDDIIKTIENDPTITHLSMVHSETTSGIINDIDFIHKLSRRVVFILDAVSSFGAYEIPISKLNIDFLISTSNKNIQGLPGFAFVIANKQILSQCKGNAASLVLDLYDQEEYMIKTQQFRFTPPTHVLRCFECALKEFEEEGSIKGRQKRYTELQQYLSQQMQNLGFKLFVDPKDQGCIITTFLFPKDPKFNFNQFYDYLAAKNLIIYPGKMTKAETFRVGSIGALTMQNMEQLIEAIKTFIAETNLQVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHT2</accession>
    <name>A0A8S1PHT2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transporter ATP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1170185</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHT2"/>
    <dbReference type="OMA" id="IMGECVE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000461">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF1">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="80"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="387"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8102213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="600" mass="67747" checksum="BD8256DD12DCD0BB" modified="2022-10-12" version="1">MQEGKKSKKFFGKDIMNLLKLMVPEKRFCIIGMTVVGLNSALLLYLPQVTTDIAKLQELKYDQENKEKSQQTNLYYHVKWGGVCLLGMFLGGARRFLNTQISNRVGMRMRYAMFQKLMSTRETQQLHSQQLVHKLSNDVSLVSTGLSQDIFVMIRGFITTIGGAAYLSYYAFPLLASASAAAAVMGGSAILLGKYMSQFKVKETQELGCLSEVSQEMLQAHRLIKLSDMEQKENQRYSAQLFRWYEQATQFSKMQALNMGLMEGGGLYALILILFQGCYLVATGHLDPELAKYFIQAVYMASGTRAMIGIYNELVKTAAIYKTILDVHPNLHESELYNQSDLTKNYREYIRQDDILKAKLLNPPQSAEHTKEWQELYEHSSIPPPSIQFKNVQYSYPNHENSTLTFNLQINSGEIVLLQGPSGSGKTTALNLLTKLLTPSSGEILIDGEPLSKKDFKWIQQHVSYVQQDGIIFNATVYENIIYGNHGYDQSLERVIWAAKLARAHDFIENMPNKYQTKLKSDGTTSLSGGQKQRIVLARAILKNPKILILDEATSNIDSQSESDIIQALMQICQDKTVIIVSHKIENYKPLLTKAINLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKS3</accession>
    <name>A0A8S1PKS3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1210092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKS3"/>
    <dbReference type="OMA" id="QYSSSHD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="4"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="225"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="260" mass="30383" checksum="EB386187867BA4EA" modified="2022-10-12" version="1">MDPNAQINERYTKLQEKLGALQFEVDNTKDAKIDEIYDRINKAQSELKDIINNYSEQLGQLSGQFQDLTKQFEKQNEQYSSSHDKKLKEIKNLENKLQKKLDDDVKANREQSEQILSNYDQQVQDLLQQIGQEIKVRNQQVNIINTTLQNDLPALWQYNVQENQERVQEDQSIVKKASQEISKLFDQANQGKSQREDAEVGIFTMLKEVVIRVKSELEEERQLRMDGHEALLSILEEAYEKMEETHAKVQKQKAALLESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKT4</accession>
    <name>A0A8S1PKT4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1210021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKT4"/>
    <dbReference type="OMA" id="YFISIVM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="522" mass="61688" checksum="4D1CE1AB4841C098" modified="2022-10-12" version="1">MKNQNYESLSFNILNCPSFKQQQTIKVISNSLVNQRLNIPLLQLNSQLEKSSIRPKLFKNIFEQTNLKEGSQNTYTYKNKTQIETYQNMENNRKQQSIIKNDFQRERVLTNQYIHEQKAQDIYKLKSETNRDKKINRPIVQLEQDFQIIQSLEFNPQRETIIQSQLYNNKNRIKISINNNKHRSYTIDGIHNKDNYQLGTIDNSKVKKKLVVKKLKLKIIAILVRAVLKLSKKYKLIFFNRNKALEHFSRLKKPHLSHFNKMAYRQIQQQYRNFVEQIFSKIIKLLNSSNYIKDYTDILKQQQELILDFQKQRLCFFIKLILQDLELITRKNIIPGFILHSLNLCLFSGKNTQTSQFVVNRTKFYSKTVHSLTQEQKVLIAIEFLFFTIIIPNMLDIVNQQVFTNQDHQAITQIYFISIAILITIFFTNHFKNLPNIESSNVKPVQLILKICEEQEEKPVQTKLILSPNIEKNEEMLISGDLKLNFINKIFIEKPAFQKEMAQVFSKIVSNIEALIDISNAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMK9</accession>
    <name>A0A8S1PMK9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1230073</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMK9"/>
    <dbReference type="OMA" id="IKLKPNY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="170" mass="19820" checksum="5C778991DC701DED" modified="2022-10-12" version="1">MLDQALQFSYFKEPPSKFLEILQQPLNGIKLKPNYFYKEYENSPNKFKRLRISTQHEGTVNNIVIDLNIPSPNFRVQHKMDYLDNNITIHSQITSQKTTPKSSRSNSPLKKLYLGSSLVAKRKLNGYVKHKKSPDPYEICRQMTKKIQYKKTETLTIASQTLYLLSKYGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQT3</accession>
    <name>A0A8S1PQT3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1270033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQT3"/>
    <dbReference type="OMA" id="LFIDTQV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="12973" checksum="14A0218508EC6D21" modified="2022-10-12" version="1">MDLESNDFLADGDNRNVKSRSKNNNNKRNNKVETLQRDQQKQKREEQQHKDEDWSDEEEKKSNAKKLEQQQKKLKQVQKQLEYKKDEEEMQPKLKGKEKRQAHVHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PT87</accession>
    <name>A0A8S1PT87_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1310075</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PT87"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="3">
        <location>
            <begin position="10"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8106610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="469" mass="56610" checksum="4415E23957F1AC69" modified="2022-10-12" version="1">MIKKQQEKKNKSTFIRKLYEILENQHNSNIIKWVENGTAFMICNKQLFISEILKQHYKHSKISSFLKQLNIYGFKRVFNQTSKITYQHLQFTQGFVNFDLPKITNRTQEQNTIDDIEFINLMKELRFISQNQVTMCQKLEECKQIQSTASQQISALNNSMLQCILISREAEYLQKFLIKIVNGMESQFQNNMQLLFDELFENVNYTIKFIIYSYSITKLTQKQQQEYQIQYYQRPRFYMEQQQNRMNLFIKFQTQNLSIYSNHGVNKSIYQKQQKKSGVTMTSYYFEMQEFEASYHDVNQAALIFYEDTGGSHQDTDKFLNWEEDLIQISKSSPSQRDYKKNICRNILRQAVKSMRRGKQFEFLQMELQKETTHFIDYYQKNLHTINGFRSLKNQLIIDQKDTSELKLWKRVFQSYMVWFLNKRASLFILNGEANNFKEYLRFKNEVMLFYTQFPEQWCSNEPSWKICN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTB2</accession>
    <name>A0A8S1PTB2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1300014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTB2"/>
    <dbReference type="OMA" id="ENDYTRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="291" mass="34043" checksum="DBC1A5125AAB27B1" modified="2022-10-12" version="1">MIKLKICETQKTKSIFKFTQALSDPNIPIEEVQNQGPQTHSNKELEMDIDQLSYQKLFSSNEIDQEIDFYDSRKSNSQMGVSPGLSITSYDLEQTDKALEIQPYGSVSYNQREVPQIVLALRKRRPAQIQIVQTISQSQESCKTLSNFSLSDLSPMQTKRLQVKSVIEFSLFENDEEEIYSVFEDELNHYGTLPQSKIDFLDNLHKFLVLYKMRAGTFPRQRENLENEMIISIAMNLAKVDKQLFKQYFPSKKVFFDDENDYTRIKKVKRKQIRQLTLVETPDFQYVKDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PU24</accession>
    <name>A0A8S1PU24_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1290095</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PU24"/>
    <dbReference type="OMA" id="NGDMLMK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="43"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="251"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="390"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="390"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8106088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="52521" checksum="04713DCC16308333" modified="2022-10-12" version="1">MFKLCYRFTKAHLPVFKQYPLHPKILEILDTKGLHTATPVQDMLFTCQNKVRLLIGPTGTGKTYAYLLPILGKLKQEEEEQNKILTEENKPRAIVVVSTKELAEQVEDVSQEFTKAMKLSTLALGKGTFKKEQSYLQEGVDLVVTTLDRLQRHRSAQSIYLSRVSHYVVDEIDTLLDSSMQDDLKKLATYFKDKDITTTYCGATYSTKVKNFVESQYKNQITPLIEKQSHMHLENLSHDFVHCTTLDKSEPFMALYKECQNKFKGSIIIFCNEISSCHFLEFFCKKNGIKTVSLHGDLPKGMRSQNVAEFRSQQCKVLITTDLGARGLDFPFVDAVINFDFPNSTSDYLHRAGRAGRAGKKGFIYSLYHNSQQSVIDELRKANEGERPIRIEESAYSKTNKEDSPKGKVIKSKSQEKQQSKTKSQKQHYVKGYKPAQKQTKQERTTSKSYFKKVHEGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXA7</accession>
    <name>A0A8S1PXA7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T1350075</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Plays a fundamental role in microtubule organizing center structure and function. Component of the infraciliary lattice (ICL) and the ciliary basal bodies.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the centrin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXA7"/>
    <dbReference type="OMA" id="IAKMMEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001132">
        <property type="entry name" value="Si:ch211-285c6.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="48"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="357"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="395"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="431"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8107900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="59119" checksum="C9F77057A10B6E56" modified="2022-10-12" version="1">MGSCFTKQQKEEVHKRHSSKLKVIIPKNQLLETNPVYAEMPFDLGDCFQLNKNLFKDEYNIIDPNPFLSTEYGIYQWCYCKKTDSRKLVKTIKKSTDPKSDEPVLILKNIHLMQYLDHPNIAKMMEYFNETEYYHIIYEDYVGGNLLSKMINKGGNPEQMAAVIIEQVLSTFVYLHSKNIIYRFLNHNTLFCDDQLIITFLEFGAAKKIQHNLQQPIGDQYYQSPEMINENYSFKSDIWSAGVLLHFLLSGAMPFDGIMASSIKNSIMRGIIKLEDTFDWDKIPEAREFVKKLLNFSQYQRPSAQDALKDKWIVKAKQKKKALKMNPALTNLRKFQKCEVLVEAIIMQIVQMTLTQEQKREIFQIFQEFDTNRDGKISTQELIEGYKKYQTSTKLEDQDIEKLVKKIDSNGNGYLDYTEFLLACQDKKKLLTVEKLKLVFAQLDVDKDNALSMIEMRRIFGGNRISDQNWHNILRKTNLQQKSVLSEQEFLEFIIKTIQNDHSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q268</accession>
    <name>A0A8S1Q268_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1410147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q268"/>
    <dbReference type="OMA" id="KFNVCRA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="101" mass="11835" checksum="E8E31D1EE595F9E3" modified="2022-10-12" version="1">MISLIQTEEQIQQQQTQSQQKNKITKQINKSVKWQEDIIDNENTGKLKFNVCRAKFIHQLEIDDKSDTCTSDDESNALERDKKSKQKHKLKCSKLNLKDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8Z9</accession>
    <name>A0A8S1Q8Z9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1500068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NQNYSQC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026736">
        <property type="entry name" value="Virilizer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23185">
        <property type="entry name" value="PROTEIN VIRILIZER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23185:SF0">
        <property type="entry name" value="PROTEIN VIRILIZER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1744"/>
            <end position="1765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1778"/>
            <end position="1806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8112166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1865" mass="218679" checksum="D61C28B776566752" modified="2022-10-12" version="1">MNYPMDHVVQLLYFDNLEHLESGQNQTRQKVKEGKVCDEIIFQSLVKVQLIRIVKPGYNVHQNLKKLITITQSEEPIRNLEIFAKSYQSNKPQSRYQLLFGSPEISSNNCDILITLEQPYITDFIVFRGQYKRLTICMYGEIMSGHSIQSYNPSYGKNIGLQELNDVIDTRIQRVRTITQPVDNKIYQQINKQRNEVLDHIYNQENYHLCVDNIQPQLLLEDKQFNQQESQKQIEILAKELKSIDQDYDFVDKQLVSLYHEKLEQMHRVLTKHLKHSKADIHSAAHFNDVDRIYKLLPTSLVDSVIFAIHASYSSAIRAFPIIIQLLSSKYHALLFLDKGGLNKLLEFLIEIQSSPSYFKLKVIETLYNCMNHVEFCNELITRNVEIKTQDSEKSPKKKKKVKKDKEKKKKENKEGKKKKNKKKSRSRSRSKSKSITQEQQQFTNEQVSNFNGFQLLAYTASKSEDEKIVQACHVALNRLQIYVRFRKLKNMIDLVYQGKLAFNEDGTMDFLLNNLPYQWEFENLVGLDLFLFAVQKDFYCDKQRELMYCLFDAEKTKTANFKLVKEILQMKDLCITYANAMWFRQMQFMKYIAAMMVLNQNYSQCCQLIYRILQIKGGIVVICQETTALKLIIRVLTKNNDIQLLNLIKSSLVLINLMDQIYIKLLGSDYDYELISLLGQLQQYTEEQDEITQNCAAVTHLMNNEFFSEALLYIMSVTKLDDIIEHEVEITIMSHIFINILRYNDTHIGLFLADRLIAPNARFIKYINTEKSRFSADFRLSQILLAGVLEPYELFLAKDTTKAIRESFHQKLKFVAINQDPDKNKLKEYFGVGEHKKGIHEELKSEFRLLGTFDQPYQSLQFLDGAIKIFKWLCNCNKFYFIPFFEHEVIAGVECFAGKLINLFSAMTSKNIQTEYMLIKLPNQQQILRHYFDLTYNTIELIQERLKFRVQLGCESSFHSNDLCKILIDLWVSLQKVQPNGLRGCKQKVENLLNILRNCFNYVITLNSFQDEDNDQLKLHAQQGSQEIQTLFQSLYLSAFRNQEEQKYILNLMQYLIKDNQNKPQFSNNLFNVLFKPMRKNDDEFENYTKNGDLGFLIDTMGDQKCLQNLMQSSLRTFDTALQNEMFKVLRNLLELYDHNISQHLFKIIEDQLKNYSNKIKALAKKEDQQLYLKYRQDWVLLGLFLTQAIDICLCNCGFMYIFNYEHLNEDMIKIYKLIDAENDYCDSQPTEKSYKLLIATLRNQILQIFKKAVDHECGVSQFLQSESQSYQYLIEDIPHITYLQDLLKSLAKNLVKHIEYSEDSKEYYHLNLISSKLIIEILECTSKNIVGQNILLYSDYTQSPYKAKPIFQLYTIVELVQQSILNYNNLPFQSVYTSIIEQFISLLFRLFVSIPNNVIHPGDVRKRAIQTLMFNKQENILDFITNLSNYLSNIPQFIKHVKLLQFLLKQIESNNKEIKDQNVTLPSEPRTHLVVFQLIQEKIQQLQSKGKQQQILSSFFKQTVLEGDKMLKYQSLTVSEVTPWKLAFKELKKESSTQSNTFIFPEVLYKDQIFKLVPLEIPSYPKTIINDIMSLQIRQPLISKDRVGFAKISSVQPALEPIRPAVPIPIRTPTVDAPPPLYKPEMMFPLQQPNVQPHQNPINSTPVIPIRPQNASSNYNLLDSIAPPVTFHQQEDPRLQRRQDYNMHPMQQIQQQSGIQQQQQQQLQQQQALQQPIHQPYQISQIPMFMPQIPNQMGSQLPNQMAPPIQQVQPPQIPQQIPPQQPILQQPMMHMHPQHPIQQPPIQQQQPQQPQQQQQQQQQPNELAELIAMMNEQEKAILREFLTSRDPRKLEQLEQKRIEYPRIDQFLMALQQQQKKKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDI2</accession>
    <name>A0A8S1QDI2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1560077</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDI2"/>
    <dbReference type="OMA" id="CKIEYHH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20335">
        <property type="entry name" value="BRcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22584">
        <property type="entry name" value="Rcat_RBR_unk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000893">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="177"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="181"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8113728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="381" mass="45457" checksum="61333CFC5049BF17" modified="2022-10-12" version="1">MIGIESLAKEIKYSLKSKNADEMIMDSYLLGFLQGKNHNKNNFAQNLELIKNLREFIEIQVYTNEQILNLYNRALNANQDLLQLCNNIEENTFKIEQFNENQFQPIKCQVIQNNQQQQNSNQQNFQIFEDEEEKNFQFAQRIQLELDTQYLKEVNTQIQMEDMIRKKKKQQEDEQKNQIECKICLEVIPFIEMATLQCSHIYHQKCLNQYCVTQIQARQFPLCCPTIECKKPMIYSDLTEVLDDQNLYEFQQFTFKQYVESHGDEYSWCPTPDCKYVFIADDAQFNCPSCTKSYCLHCKIEYHHGQTCQAYKEKIQNELRQKNEKILDDQFFQFVQGAKYKQCPQCKFWVEKNQGCNHMTCRCKFQFCYVCGGVYGRCHCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QM71</accession>
    <name>A0A8S1QM71_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1740019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000183">
        <property type="protein sequence ID" value="CAD8116546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QM71"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="115" mass="13273" checksum="04C606B3E9777843" modified="2022-10-12" version="1">MYKLESFWSLNSNNKAQTPILIKVFTNYLQIISTISTFQLQVPAGLASVVGNPIELLAYFLDCFLFNIKDILIIYFLLNWNLIMTSSYIIVIFTIVAIAIITKQTQFNFSLYLHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QM90</accession>
    <name>A0A8S1QM90_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1700010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000179">
        <property type="protein sequence ID" value="CAD8116191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QM90"/>
    <dbReference type="OMA" id="TNRSYRP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="521" mass="61736" checksum="30501E58F3AF9E5E" modified="2022-10-12" version="1">MLQLLQPGQFNLHVTDKNAVFSPKLNDHQPQPYALKIKNKAIQLDLTQQGKEGLTDSHLTQTNEHSQNQLLKMKLYELENVSLKQQNEFLSREIGQKHKEISKLLKLCQNSQLPPVIRQKQFTFFDQEFQEYLFSSSGIQQNGNDSKRTQSYDYQSQSKNDYQSDSYAYHNTNRSYRPSRIQSRNSKELSKYQEDFSQLPNIRLKSTSREKQIQQRRKRSLLNVSRIKVLFLYVLAYIRWSKNYRQKRVQKLQQLKQIRQKYTKCLETISNQDVLLCKGIIKQWISNIIDPLIKSCSTKQFILQSNENSKNPNSESSIQCRQNQLMLLTIQILNNMEKFTEKDKIPELFQTSLFLSLFKSQNIKAPLFVAKRTKYYTKNTIKIGIYQEKMIISEYFIFRLLISNLIESSNNMIYQNINHKMQCKFIILAIMGILQILYQDYFSELKQLDQPSTELFQKRIKISKQQDISIIIDDNIDQEESLIFGLHCKKHFENLMENNQEWLQEICLKFAKILNNLHSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPQ4</accession>
    <name>A0A8S1QPQ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2060010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000215">
        <property type="protein sequence ID" value="CAD8117669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPQ4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="207" mass="24706" checksum="A443AC11BA63DAFA" modified="2022-10-12" version="1">MGNKSAKVERQDSSELLLSQHTNTIDSSQVENSKIFVQENKLSYCFACCNCFGLIKTQVVEIQLSWIDYTLRKLNRGQYRHFQWSDLMKLRLQNLDIKNFEELQAIYLNQIRMIISSYETNSSFYKNKIAEQNEQSKYNIFEYYEEEANTIFEDKQGELFTNNQKLPIIHLDQSFKYFKFILQIILGLKLEDFNQPYQLKNKRINVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QTV0</accession>
    <name>A0A8S1QTV0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2350005</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2350006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000244">
        <property type="protein sequence ID" value="CAD8118353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000244">
        <property type="protein sequence ID" value="CAD8118355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QTV0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8118355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="187" mass="22342" checksum="E2412FDF760160AD" modified="2022-10-12" version="1">MQRSPNQKVTHICTYNNDCQKKLCDFCLKCHSIDVPFEYLLTIEELINRIDILHFRILRTEEMLFAGYYGLNKNIDIWKFQGQHEIINQLQQTIIEVNFFDEIGIKFLRQNIQIMKQCLTSIKQSLQAGRETMIYQYGILNVKDKMQEQVDQRLQDWDMDRIINSTFLVHHSSFNYLVLAKLNTKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QYI7</accession>
    <name>A0A8S1QYI7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbohydrate-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T3370002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000346">
        <property type="protein sequence ID" value="CAD8119560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QYI7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09620">
        <property type="entry name" value="CBM9_like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8119560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="23054" checksum="0A5E86E5187D37C5" modified="2022-10-12" version="1">MILIFAIIIVISNAVIDPITVENCKWGPTQYKLAHQNEGTTENVEFTYFTICKSLNHKLVIDFFNVDSFIDAHLTKCNDPLYKHDVDEIFIGTKTEYLEIEISPNGALFYATIGNPNGTCSNLITTYRQCDEITYFAEFVTPTAWNAHIELPISMFTQPIYANFFRVNKMKDNSTHYMSYNPINASPACFHRPDKFIQLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMC7</accession>
    <name>A0A8S1JMC7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMC7"/>
    <dbReference type="OMA" id="PCPQFLN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="181" mass="21713" checksum="7FBB36E9CE0F3160" modified="2022-10-12" version="1">MNYLLKQINEIKFDKKVRSNYQLQKYKKKQIYIKKQKNKKNKKMSQNYEQWKLDQMKQKDFSKKRNKNVDSEGYFGKTELNNGIDYKKNNNPYLDDCVDNQNKQRKSDNDMNWHNAKKDREPNGELQVVLNGNVVTILKQQIKTNQKRQNRKYSDQETYAMSNNISGPKFSDIPCPQFLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-09-13" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN12</accession>
    <name>A0A8S1JN12_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Armadillo-type fold</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040394</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1383" mass="163026" checksum="9D1E5A1DE9402390" modified="2022-10-12" version="1">MNLIDSLLNEFQQLLNDQKKNQVLKPLIQGIIDEIKKHKSQQNLPIQEILKIFIKLPESKSNQTSGIALNIILKFINSNLLQKADLHLLQQFLYLLKDEINENVQLKLVQCSVHLINYDIVNINNPESIEKLLFIFFTLVNSKNPIIRTTAMTGLMEILDFIYISYKDEPHKNGVTIFLQLIQGMKGQKVPFIIQNEFSKGICRDMIYQIIQILGCFLNKDEQLNKLIEEFSGIIYEEMSLDSADIQFNARRIRDSFSMIVTLQRDISLLKHISLLFTRSPQYGYVRYWILEGILSLLKDPKLVMILYQGSTQEEHQQQQTTFLQHLIFIVKLSTEQEPQYTLNQSNQQQTYAKQKNVYQQNILNLTEIPYYNKSLFTQKSIECISNFTDSILNIANKNQIVFFNQNYKQVQSKIIVDENGKEVNQIIETTQQLFLKTIQNLMTNLNDETQYQTILNSLQNWIYLSNSLGQVKTREAFIKYLSSLCVTKQNITLTKYQLQSAKILFKIAQIGNLLDIKSWYIIMKTMQQFDTLIQKSQTQNAIQQEQYHEVYQQDITQLNKILEGLFSSSNEYEDSHLLQMIEAINQVTLSLMEQFNNVQNLVDSKSIQFGLQKIHQITKQNWFRIHKFWDFITAHFLCIANYKHKAFRETALEIFSQLVQQGFIYFLRPDQSCCWDGDSWQSHLLSPIQQMINIPYTDVKETLLNIIFKLIQNNGHELNILGFNTIIEILLISCDGTEPTGYVNIGFHILELLIGQFMHLLDPKTTRRLLPLIKQFRQRTTEQNINYVSVGLIWQLADNLNKICTNQTSQIEVEELWTVVLQSLKDLSLDNTPDVRQSALHIIIQIILINCGSFRINFQIDLLKNILFKILDDLIGRVAQLQQLQVIFHMKIPKYLKEEDYPKFTKFSDQDLQTSIKTQNEVIIAWEETLKIMIQNLTKFLKKIGQQEFKQQAQQLYNETFIRLIISFKINNLDIKWEIIRLIKENTEIMIEKNLVASFDWAKDFIICIQEFIGQKIQDNRDVKTLINKILPEVCELYVLFLKAHKLEQDQFPEELIDNLYESYQAIVDLPINIENIQNIKIWMDEKQLHDYAEDFYVYLNTQQDKSKFYNFLLFNIKKEGGMTKLQDLVIYKYIQLLNTYIEDKQLTDPDLIKQYVEICLIQLSTRQNQQKVNNVKNYNKTSKPLWVQVLPLIIQTIEICQKEEYVQLLSEQLNFTQQWDKIISIDGLKSCIDVELQICEWLLSQDILDREIINYWELLSRNINLYQLEDLFYETRQKLMNIVFKKHKSLYTLNQICQSLVTQFMKDEYMSGSMPLSRKRVQEIIQLLLELQSLKDLNLEPHPLLQVFECLVELITTREIDIKLPLQNLFKSVAFKIRTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNH5</accession>
    <name>A0A8S1JNH5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNH5"/>
    <dbReference type="OMA" id="YDYQNIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="338" mass="39443" checksum="F23E047FB2E99A69" modified="2022-10-12" version="1">MKIVMRSSKLEQLMKFKGKHDISCQEFKQLIMDTRQQFTQLNGEQLNCVVDLMKHDQLVDDDNTDYLLTAIKNVEDQQIKYQLLDKIILPTSNFSKEQVKQIEQIIENCQQYAMGLSLMGKMVAFYDYQNIQIMNKFIKEFDVTKTNAEQLGAACIGLALGQYKDKQLWDDIAQHCLKLDKDFHSYMRIATGMGLTNNGNVEFWNDFIEQFRKQLKTINEDLFTQSLQVISQQNVVSLELCEAEIEYYIINSHIRIENMMSIYVSMARKQLGSQQLYSKLQQDIYEMQLSGDDLILYVIELMQVKDRLPNQTLNVLVAKAYSESQNQELIDKLTNLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNS7</accession>
    <name>A0A8S1JNS7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNS7"/>
    <dbReference type="OMA" id="QITNGHF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000289">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="66"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="217"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="614" mass="72393" checksum="A2FE9B027DD1A13C" modified="2022-10-12" version="1">MKQSNQKNKQAELLTKQQQQNETLLTPALNYFMNPTLQTTELIESLMHIQFLKNNCPEGMQLHDFVIQAAKYVKFETFSFGHIIFNYGEFGDKMYIILKGQAGVYVPKSKDDIENEQNLQNIKKSKKMLFQDDALFENRNNQNYYQNGIYLYHKVFQYFSGQCFGDVALTSDKPRSASLLVISEVMYCISINRQQYKQMCEKAIQEQNKTMELYQRILHGISQFSISKFIQNLRPISFVSQSILWEKGQEPQYLLIIIKGRVEIYINLDDSQILQNKSIPGLFTIKLKRVVLSQITNGHFVGQEELLEDIPQRKYNCQCVDNTEALFIEAEAFKKIRKNFPDIINLLKNITTQNKNYINQRLTTVVKSIQNYQDKINKEPLNKIDKRTFYECLDLQQQMNQEQTKVLRSRNKKLFTLSQSIKKKVETFQQKYKDKSPQDLIITNPELQQTIQERQDRLYYINNKKLQYNRPRHQEGFSLSNEIKNFKLNFSKSSQRFQKTDQNQMDSNFIQYSSQCISEAALSPVSKNSLQFQLTQFSDSLKKKQNFLLKMQKTMRQSNKSYLSIRNMKSLHSQNNNGKMIKTEVSFMNEQCLNKYERSLQSLPKLLLQENEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP42</accession>
    <name>A0A8S1JP42_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0060325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JP42"/>
    <dbReference type="OMA" id="CAKNISN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="174" mass="20451" checksum="ECE01CEB595DC22C" modified="2022-10-12" version="1">MGQIQQICAKKLSNCDSSISDIRQHKLKRECFRDQSSKMTFDQSTVITETLDELKLKLSQDEDKYNQDVETNISIVESFQKLETKNLSQRELDEITDLNVINKSDGIKSRSKSVFIRNTLPEIPPKSILKNREIQQDQEKRNFTQQKRVRFSIKELQKNKLLSKYNGYKKISYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPH8</accession>
    <name>A0A8S1JPH8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPH8"/>
    <dbReference type="OMA" id="FKQISGN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="383" mass="44042" checksum="33385C717C98958C" modified="2022-10-12" version="1">MTDLIKQLDGMFTREHLEKNKYLLKNLDPNLNLPIVALQKEYSLAKHSIEEILGALKQCKNCKLHEGYVTLLIPPHTKNIIINEISQTNADFKAYLIEKYLKNVECEIAFEGGCCRVTFKDDEVGLKFLDQAVNDPKDPLKAYVEPENVYQSLTKTLGFNNQAQYLYQYNNQMFQNLYIRKASDDVAGQNQSEKINKPYYPPVQQQQSNPRKQSGQERKGSEQVEHQDKKTKTPTTGPKEIYVEKQSLEKQEQNVKNVEKPPKVQQSPYVEKKSTSRKTSEQIQPAKHKGSKDIQGSPIRKASIQYKKDNLIEIFKQIQGTLKANPKLQKLSEQDRALLLRQNGELTLEAIHPTPCIRKESYASPQQHIKQSPAQIPQRKPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRP6</accession>
    <name>A0A8S1JRP6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040113</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0650227</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRP6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="151" mass="17462" checksum="4375AC1A5F42C72D" modified="2022-10-12" version="1">MTVKIVGMVQCLLIWQKQNLLQNLCIRNFIKRYGCILAISEKNLDREIKILNLNFISNNGTKGTEVQVQNLRISMQNSRIINNFAFDRGEGINFNLNSNRFLLNQIIICQNSALERSGIFLQGNSSLNLENFSQITQFISFCYFIFNNINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JS53</accession>
    <name>A0A8S1JS53_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPRIM_AZ9-3.1.T0080396</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="13 14">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasmic vesicle</location>
            <location evidence="14">COPII-coated vesicle membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
            <orientation evidence="14">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
            <orientation evidence="14">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JS53"/>
    <dbReference type="OMA" id="FPPHYAE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.730:FF:000005">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000001">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000041">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000043">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF0">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 14" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="15">
        <location>
            <begin position="65"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="16">
        <location>
            <begin position="132"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="18">
        <location>
            <begin position="393"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="17">
        <location>
            <begin position="507"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8044827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="752" mass="85870" checksum="360E2F4ACE64D222" modified="2022-10-12" version="1">MQQPQGQQQIQPLDFYQEEYKDAVRLSWNTLPATKLQSTRAIVPMGCLYTPMKDLESLVLVQYSPLYCKCGAILNPYNQIDFRNKQWTCIFCSCKNMFPKHYADHITETQLPAELKKTSTTMEYILANQQAAPTTFLYIIDTCIPQEDLQAIRDSIQQSLSIIPPDSQVGLITYGRNVFVHELGFQECPKCYSFKGSKDYTTQQILEMLQVSTKPEILKRFLVPLSECEFSFNSILDDLQCDPWPTQPGEREVRANGSALKIGSTLVESATQFSKILYFVGGPCTIGPGQVVGLKLEETIRSYLDIQKENPNTQYLQKAKKFYNEIAQRAIKANLTVDIFAFTLDQFGLLEMKQLAEKTGGVVVMQEKFDSDMFKETYKKVFDKDSSGFLKMGFGSKMDIFVSKDIKVQGGVGPCISLKKGGPMVSEVSLGEGGTTSWYTGGLDRNSTILFLFDLSSTKDTSFSQCAYIQFVTMYRHSSRLQRLRVTTIQRRFADHNNIMDMIRGFDQEAACVTMARVGILKAEQEESIEVLKWLDRSLIRVVARFGSYRKDDVSSFRFPQEMMMYPQFMYHLRRSHFITTFGASPDETTFYRASLSRESVQNALVMIQPALLQYTIDDPVANAVNLDIQSMKPDVVLLLDTYFNVVVWNGENVQKWIEEGYYDNPEYEYVKELIDAPNSDVQYILEDRFPVPKLIKTYFGHGQERYLKSRVNPSVNTVQNENVESGNYITDDASLKVFTDHLIRLAVQTVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSM9</accession>
    <name>A0A8S1JSM9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSM9"/>
    <dbReference type="OMA" id="TMSRYSL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026508">
        <property type="entry name" value="TMEM164"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20948">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20948:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14808">
        <property type="entry name" value="TMEM164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="32665" checksum="98693C2722DC4FCE" modified="2022-10-12" version="1">MDIVRWGLYGNIYTETYEHFSDGGPKCYQDMTNTFRISISTISAIICIIVMKKCWAKIKYSQELTRAIGKKPNIFEYLVGLACWGTFAVQTGYKLYTGRGVFMNNPCHIVLLMQGYVLLTKKHKFSAIVFICQLRWLYGVFAALKFPVITGLELPLEVEFFWIEHLLGAFAGPLVLTLSGRFFFFEWKTFFIHQMFGWESHVLYQRIYMLPISLMTWANLNFMLCRPDHDPFVPYIGNWYYVFSEFYLNLVSIMAFVIVFILTWVLRKIIRYKEKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWN1</accession>
    <name>A0A8S1JWN1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CSC1/OSCA1-like 7TM region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0110026</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWN1"/>
    <dbReference type="OMA" id="QIRIWRT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF5">
        <property type="entry name" value="RE44586P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="405"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="449"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="530"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="2">
        <location>
            <begin position="311"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8046647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="585" mass="69051" checksum="C8FF8928E1B12266" modified="2022-10-12" version="1">MEYEQSDYYLLLGSSIFINITLIAITIFLWKVIWLQYPIFIEEFQIKSLSDAYNIIQTANTNLILNQCGFDGLTLLLFEQKLFVGLSYYTIFYYLIMGICKISWNYLNDYQKLFVQLNILPNQDFIISSMLLLVIFAILIQIRKELQIIYIQMFTTDQFTNYTIRTLHIRGMDYDDYDGVMMMLEILKYLNDVGDPGTIMGISIIPEYSKLLELEKNRCLFKYQLGILELQKPLFYPLPNLSLIEQQIDQQLQKPFKLSGHCFICVDRLQTQLKLCNQNALTQYQIQLAPDLYDINWVNFTIESNQIRIWRTIILNLLIMTLLIFVTSPQALYQYLSKFPGLEFLSFKWTILIPEPFGRIIKNNIPPIILISINQIILYLLDVITQAEKHERWSQYHISFFHKMFFYLILNVLVIPAFLLQSSETLFNITYNGFNIQLQKAFDNQTNYGLYYITFLFYSGTGAFLVELIRPSELYFNYLSSYMAYYMRLYENDAQHYQKSNEFSVQYGYISAQMLLNLTIICIFNTTSPFILIAGLWYFGFRFIGDFFMLMVSKQEMVTSYNQVVLHWDSGLLSKLLVAIFNLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JX63</accession>
    <name>A0A8S1JX63_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JX63"/>
    <dbReference type="OMA" id="DQDDKYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="77" mass="8902" checksum="1B893EBD24B41291" modified="2022-10-12" version="1">MNRNFINLRVQNKIEFDAIITQLDNGSPLSYNQVKKFSGIKNLDLFETQDDKYETPKQSSNSLLDEDSNIKGKMKEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K086</accession>
    <name>A0A8S1K086_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acyl-CoA oxidase C-alpha1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K086"/>
    <dbReference type="OMA" id="NEQAFNG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003997">
        <property type="term" value="F:acyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055060">
        <property type="entry name" value="ACOX_C_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF250">
        <property type="entry name" value="PEROXISOMAL ACYL-COENZYME A OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22924">
        <property type="entry name" value="ACOX_C_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA oxidase C-alpha1" evidence="1">
        <location>
            <begin position="258"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="599" mass="70505" checksum="7BF30645DAA0A307" modified="2022-10-12" version="1">MNSEDFQVSKQSLTYLIYRGQPQFQLFQEFKKFIQETPEIQPQIIHSSHNLNEQYNIDLIQVDRLTKFIQSKGGLYQAESYVQYFGDFVSPNVQRFFQIQLLNLRILKTITQNKELQNLLAQANTLKVSLSLLSNEQAFNGSLQLSELSTSIQFENNQATLYSKEGIYKIGNNYQGKFGTHALVTCKNQDKIYLALIQLKDNKVKEKWLGKTISIYQNYAIQFEGSKVMKYIELNQEDLELIEQWESHQGFEDCQIALREIMRLTDICYKYCSMRKQFRTTDDKTERPILTYQVTLTRLINAISFAVMFYISVQEARKEFFQDVFMKKARRKVSKKFVDFFSLYITEHSVYVAEQLRDVFGGFGFLRISGVPQIQEKLIWLSSFYKNNRIQKQTDYLNTILDVEKNKQVVLYYLGQYFMGDPVSMFAELGPLTQSNVSNPFHVFGYHVAKIKEKLLQQEHLKYSYSAYQFSQLTFELFASQKFFALLAFRRYRASNFILDRLLLNQNFISLSLMETNITNINLIKQQIGQNAQELLTVLNLLIDSYEFEHYGILLGQNDQEKFYQKLIHASKYENNRNLEQVVNEIRLPVQDLFNRPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K100</accession>
    <name>A0A8S1K100_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0130263</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K100"/>
    <dbReference type="OMA" id="FHGTGKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="19"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="62"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="104"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="238"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="284"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8049150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="317" mass="36220" checksum="4C64D7D721F16267" modified="2022-10-12" version="1">MQSQENWLDNSIEPFQKFQQAHSNSIECLQFNPSNQSELATGSHDKLIKIWDVTKQKETAKFSGHKEGIWSLSYSLDGKQIFSGSPDKSILVWDAKSGKNVQALKEHKNRIYWIQVSDNGLYLASGGQDGHLILWDLRKLKLIKDLQISMDIVYNINFSQQSKYFFTGDSMGVIKAYDSQKIEEISNTKPAQKNKCYAIQSLKMSEDNYKLFVASKNQSISEYNFDGKKKELTKINQSAVHCDSVHCINFDKDKSRFGTGARDGAARIWQTERKGVIYTPLYNLIGHKQRVTAIEFHGTGKIIATCSWDQNIHLYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4S8</accession>
    <name>A0A8S1K4S8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YEERSHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="981" mass="117567" checksum="D4094E827F2B3A73" modified="2022-10-12" version="1">MYQNTLQNSIKRFDKLTTSETKYSRQSQDSYYLKNKQQTQLAIPSFRTLITSRCKTQQTIEIQQDKSPTTTQRRILKNKDEITEKLIPRSNEILEIYDEFEKNYNNVKMLNSLTSELNYLDQQFYTNINKQPQENITQVINYQNNYLTQCDLNYEMKLEIQRFRFQYYRIRIKDQESPIQITFLSQNAQINSLRSYFSLKIEFPTKFNADYQVNSKSIRIFANNTTSFFTEDYLFMTLYSQNDFLCTVVVTFGFERKIKVNKPQKAQSLFEDFIPRTKKQKSQPKFYQPEARLQNLLNPETLQQIKSERQIKQQKVLNNGKNIIEEKLLDKKSKLFVKDQIIKYREIEKMTKQKVSYRNALQKAWLKCIFTTIFLHNLQYFLLCQKKQIRLEARGKLLVWQLQTKSLISVKDFGDTPIERTIFKSSLSLNAICMILKKPIIQKAKKVVTSYLQSIILAISVLNKYQRWTAKIISIQRNFRNMKCKKRNFKEKLWRLIKEEFDDIIYEFAQLKGITSYYDYEERSHIHIDTVLMFSLLDEYAQXFRFQYYRIRIKDQESPIQITFLSQNAQINSLRSYFSLKIEFPTKFNADYQVNSKSIRIFANNTTSFFTEDYLFMTLYSQNDFLCTVVVTFGFERKIKVNKPQKAQSLFEDFIPRTKKQKSQPKFYQPEARLQNLLNPETLQQIKSERQIKQQKVLNNGKNIIEEKLLDKKSKLFVKDQIIKYREIEKMTKQKVSYRNALQKAWLKCIFTTIFLHNLQYFLLCQKKQIRLEARGKLLVWQLQTKSLISVKDFGDTPIERTIFKSSLSLNAICMILKKPIIQKAKKVVTSYLQSIILAISVLNKYQRWTAKIISIQRNFRNMKCKKRNFKEKLWRLIKEEFDDIIYEFAQLKGITSYYDYEERSHIHIDTVLMFSLLDEYAQKKKEIWQKYIHDTFVVNNPKNRLISLAINFKKPSLFQLPNEFEIRDIIEKYAAIKRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6A0</accession>
    <name>A0A8S1K6A0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0140217</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the citrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6A0"/>
    <dbReference type="OMA" id="VLEWLFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06103">
        <property type="entry name" value="ScCS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007128">
        <property type="entry name" value="PRK09569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF8">
        <property type="entry name" value="CITRATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8049893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="455" mass="52363" checksum="CF2C275457506D93" modified="2022-10-12" version="1">MQHIFRSFNSKSIFHSSNLLKERLREIIPIKQALLRDIRKTYGQKEICKVTVDQAIGGMRNVFGLFYDISLLDSKTGITFRDYNIPEIQEYLQKAKNGNEPLPEALFWLLLTGDFPSDQEFKFVQEEWKRRGELDQETIKFITSLPKQLHPMTMLSQSVLYLQKDSTFQQAYESGKATKPQYWEYYYEDAMDLVAKIPRVAALIYRHKYKNGDLINSDNSLDWAANYSHMMGFNQFNAYELFRMYLSIHADHEGGNVSAHATHLVGSALADPYLSYSAGINGLAGPLHGLANQEVLKWLIEIREKLGDKVSNEKIQEYVLNTIREGRVVPGYGHAVLRYTDPRYIHQKNFAERLIKDDPLVDLVKQCYHVIPPVLKTIGKIQNPWPNVDAHSGVLLNHFGLKEYEYYTVVFAVSRALGCTANLVWARAFGLPIERPGSITLRWIEEKFGENQSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8Y9</accession>
    <name>A0A8S1K8Y9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Basic immunoglobulin-like variable motif-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0170347</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8051013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8Y9"/>
    <dbReference type="OMA" id="WLIVADC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171:SF12">
        <property type="entry name" value="BASIC IMMUNOGLOBULIN-LIKE VARIABLE MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-G CELLS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="44"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8051013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="45050" checksum="C52ECF6A634C91AA" modified="2022-10-12" version="1">MNSNQTDLHEQQVVENTENIDTTNTEQLLDFTNMFKTQKVAKPKLLNRQQQQQQQQQQQSQSQSQSQQTQSTEEQSNKSVQNTVPRNLTITQEKMSQRRCLDVRRWYSLSRPQYQRSCGISSVVTCWNYLFSTLGVGNLNPLSQEEVIVSLGLTSEVGPHFNDVEFGSFSGNMSLISWFKNLCRLHKVQGRAYFLWKNEGDFATPGVDRDVALQKLTAGLKTDKVSFIYHAYDHYFCPIGYECTPNKQVEAFADEIDYNDCQYWIIIAEPAKPYPMFTVRKWVDIAQDLELKYPQYMNIRKMDEGIKNYKYEKGVSQHCIMAFERIDPKPKKIIEKKEKPIIINEEVHLEKTNQLNQKVEINEQIHQNTEILDPLINNQLKQNDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAW2</accession>
    <name>A0A8S1KAW2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase S11 D-alanyl-D-alanine carboxypeptidase A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAW2"/>
    <dbReference type="OMA" id="ALDMCRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.710.10:FF:000097">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581">
        <property type="entry name" value="D-ALANYL-D-ALANINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S11 D-alanyl-D-alanine carboxypeptidase A N-terminal" evidence="1">
        <location>
            <begin position="20"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S11 D-alanyl-D-alanine carboxypeptidase A N-terminal" evidence="1">
        <location>
            <begin position="157"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="319" mass="36025" checksum="91137A4C07937034" modified="2022-10-12" version="1">MNENKSLHAKRQSALSLLNPPQTTALSWCIVTSMGDELSSKSPSLKLEVASLTKIMTAYTAIQLCEELNLDYNEIYIRIPRIATQIGGTSAFLRRDDTLSLEELLYALMLPSGNDAAISIALHFGSILNTQRKQKIASYITCDTIQGFYDDTQMSNMNRFVRQMNENALKLGMRDSQFSNPHGLSSKANKSTALDMCRMSSAYLKIPILKQICNSKSYSCRNYEWFNTNQLLHFENYQGIKTGITPNAGACLAVSYESLLGLIIVVLIGAKDQKRRFEETDKLINWISREYTLYFNKTMYAPPRTAQSKGRSFIIKKQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAX6</accession>
    <name>A0A8S1KAX6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1079"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1117"/>
            <end position="1136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1137" mass="136403" checksum="77114C6BBC7A3211" modified="2022-10-12" version="1">MNKFEDDLKYAQKANGNEFLNWLQNLSFELIKQKEYSYGQEFLKFQHLVYELIFKQDGILNSSFRTTQMRFGSIEDTLFFIKILNALSPNEQSFYQSLPSEYPFNPVQIDILPAHLQLFLKDPSIINMNSQFQTFQEIFQDNILVEKNQLKFCVSAKMMFWIHLLNGAVNFKESSLNATENSQKILDCAFSKCKKKFNTKDFLLKQLTFNPYLILIRRIIEYMVMQIKPGNPIQNNQNKQITIFTFQQMIILLQEYSMYEHLYVQKLLDLNLTNEQQKLFYKIKPSSVVLDTQMMLIYLTIFFNNRYGQDLEATYSQFKQFIKSTDILSKQSQIFYQNRGFFFECGRQKQQITYQQGLYKFLENSFKFYHNDRQCNQISLLSHLSTYVLFLRQQFAVYEISIYNQVLIFDQALLSCLISQNTILNQLQKQQNFQYKSNNPIARFILSELQPIYQLCKNKFGQCPQAILFKEYRPSNLLQCIESKFNAQEYEINFHFREIDQFRNQMKNLLRVHYPFYTKLLIGIFKCINNLSYLSDPEYQGIMHLLTFIYSQDQNSFFCDLLKFQQNQPYQIPAYFEVKNFCDIYIKDSKEEEFQIKQNVNIQKWIQQIIIQLIQYQQQKNIKNDRIVQYLGEQFNIPISTIKVIPIRNXIILLQEYSMYEHLYVQKLLDLNLTNEQQKLFYKIKPSSVVLDTQMMLIYLTIFFNNRYGQDLEATYSQFKQFIKSTDILSKQSQIFYQNRGFFFECGRQKQQITYQQGLYKFLENSFKFYHNDRQCNQISLLSHLSTYVLFLRQQFAVYEISIYNQVLIFDQALLSCLISQNTILNQLQKQQNFQYKSNNPIARFILSELQPIYQLCKNKFGQCPQAILFKEYRPSNLLQCIESKFNAQEYEINFHFREIDQFRNQMKNLLRVHYPFYTKLLIGIFKCINNLSYLSDPEYQGIMHLLTFIYSQDQNSFFCDLLKFQQNQPYQIPAYFEVKNFCDIYIKDSKEEEFQIKQNVNIQKWIQQIIIQLIQYQQQKNIKNDRIVQYLGEQFNIPISTIKVIPIRNLSISKSTGRLSRAKIQINQWKAPLRDFEFYFLFVLMWYLSLGLDKFRGQQINEFPSTQWPRKLASPVNLLIVSLLTYIVITLVFMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEW1</accession>
    <name>A0A8S1KEW1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEW1"/>
    <dbReference type="OMA" id="TAISFRC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="226" mass="26571" checksum="6C34CCC2A16B482D" modified="2022-10-12" version="1">MILITSLITLIYAAYDGTDYLKGEGSFNIEQNWIMNQTEEYMETQFVTQGNDKFLKLYKKAESELSFKDGFYHLFEESQPQHIRFWFASGNKEIEDTNLRLKDTATDKTAISFRCGYAGFVRVNDNQMITFYNSSVSTDSKIIWTQADILLNWDTQEVLVFMNESYMGSTSFYHQEVTKVNEVMLYNLKPNTTSWWKNIKICKERCDNFEFTQIVVICFTLFILLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIL5</accession>
    <name>A0A8S1KIL5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0220309</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KIL5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="122" mass="14796" checksum="446F4463045C7587" modified="2022-10-12" version="1">MLKRDKKQKVDKIVQTNMEEDPRQFYLIDTQEEISGQILERLRQQPIVQMKKVPFDKEHGNTIDSVPINIKSHVKSENELYFELQYQNPNLLPSYYPFYFLRERFPKLLIEYYKNNSVLILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKG0</accession>
    <name>A0A8S1KKG0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200399</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKG0"/>
    <dbReference type="OMA" id="HQWIDSR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="528" mass="63381" checksum="98281563D0833E40" modified="2022-10-12" version="1">MRHLSISQSQISRSKIYTSSHPESQFLQLDNDILEIYDEFEKNYNVIQEIDKTNTKIETKQKNIEQPKIKSQQKKLLKTFDGLQQSDLEIEFINRIQIVRFRFYYFRIRIKGKQSPLQILLSFPQKITSTQYKLFVSTSIEFPNKFNADQQVQSRFVKINSKQNSKFFFEEYVYLTFYGEFDAIIGMQITFGEQYTASNGQKQIQSMINFDIFHERRTIHPKDKILQNIEMAKTKMQSKLDEMIKAQHRHTKSQETIQRSKLIQKEVRQTRLIKLLVKDQIAQFREVEKVHQKEQQLQILVKKNWILITTIFNVSRQIHRRLEKVRRKYKIAARAKLLVWQLHTKALIEVRRYGDNPFIRSLSKSSLVLSTFTSYIQEKCLQKAEKIFSQFLVDIILYQTFINKHIQLISKIKCIQRNYRKLRLVRKNFKDKFIKAFKEKIYALYQGRGSDQKPKINIEQHIISKLFEEYSAKIKHQWIDSRRAINVLSVLKKSFSVQRQDIKLYSLPNEQEMQLIIQQYYKIKKQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KR47</accession>
    <name>A0A8S1KR47_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-guanine(15) transglycosylase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KR47"/>
    <dbReference type="OMA" id="VPHIAHD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050852">
        <property type="entry name" value="Queuine_tRNA-ribosyltrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46064">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE ACCESSORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46064:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE ACCESSORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-guanine(15) transglycosylase-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="374" mass="43243" checksum="0370ABAF8478C045" modified="2022-10-12" version="1">MKKSPLFIYTTRMGAYYHLTPFNQQSLNLDTCLISAGDFFHMENTLNKLQSETNISFRQYINQENKTLYMTSIDSYKIKCVEGQSNQDGVKIAQLEGIKPITNQQYNKLARLINPEILVSLTEQPGMDAGQKSMKRSYQKSSKFLKESLDELKDTQISIYGAIQGGLDTDLKIIAAEEQKFAIDYPNFRGFTLYGFNKGSSWQDKEDALSVLQSIYDGYNLSYILAGYGDILSILWGMSWGIEGFEVEEPFRFAQDLKVILIPNFDAKDSDIEQFSGDKINFYELEGKKINYINFPINSDVKENVPLNPDCQCFACKNHTKLYISHLVECKEMNAQVLLTIHNTHQYQKLQQKLQEMQNQKQLNQWIRWFLQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSQ1</accession>
    <name>A0A8S1KSQ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="44160" checksum="5249C75F078DFF7C" modified="2022-10-12" version="1">MRQRQTYQPNYLQISQLEKPKRYVHSYGKELKTPDSIKPLSALMNLRLFLYKTKQKLDYKQASQFTRNDPLVKEQPSFLYNKLQIEQDNQIKFQAYLDGDLQADEYLTLQNYKRLYKITKNDNGSFDNTNDDIPYYTQRESALKKLQERIIHRHNLNKRKRGSEISSQIDTSKCDTPSIQLAKQLKKVIIRKHMNKINEELTAENTLKNLKMVQNANFAVQQLQETLEKDKSLQSSVQKKVNSTKQLKIITRRNKTEIGEDNQDNRIQLSNEITNFANNTIYESQIKDKSKQLKSKNLRFSNAKMNQIFIQKSQXEIASIYMANQQLMLIIFLLIFNMNVSMNAHQSSQKCKWRYISNLNECQFFRLNQKNMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUH5</accession>
    <name>A0A8S1KUH5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUH5"/>
    <dbReference type="OMA" id="KIRTEVW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035940518" evidence="1">
        <location>
            <begin position="16"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="217" mass="25472" checksum="C3FC066EC3406021" modified="2022-10-12" version="1">MILLLYIFIASTHQAQYTLTVKKGDQYETPTYPAHQKNLEVVQIRIKLGLTAATYVVPHNENQMRCMTSWNKLYGYSRCLGSLHHKDSDRFVFRRAQSCLKYNEQGVYEIPNCQEKDLVEIAAYAYDNSVVPYEHPDQLIRIFNTKIRTEVWYQYRLIFLADSTIYELMDDNGYTLEKQTIQHRTCYAWKNGYRLNLYFGGVCEAPVDIVADHVDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUJ0</accession>
    <name>A0A8S1KUJ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0250333</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUJ0"/>
    <dbReference type="OMA" id="EWDAKLM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035717940" evidence="2">
        <location>
            <begin position="17"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8057223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="112" mass="13519" checksum="6D199211E14BA8EE" modified="2022-10-12" version="1">MRFILILVVFVALVMSYNKKDSCQNIQKFTYDEWDAKLMCPNPLAPILETQNIPKTKCSRFKPNFDSSNRRLNTQDVEELRQKMKQKKEGNLKFDHDQQYRQFKEQQRKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXZ4</accession>
    <name>A0A8S1KXZ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphodiesterase</fullName>
            <ecNumber evidence="5">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0290334</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="5">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8060058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXZ4"/>
    <dbReference type="OMA" id="RDMVIQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="PDEase" evidence="8">
        <location>
            <begin position="279"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8060058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="629" mass="72995" checksum="C22C010DE424C6FB" modified="2022-10-12" version="1">MTTHNYSSQFLISYQQLHFNMLASRSDSQLRSLENPDKQTMRGESSKRSDKLLEIPRKKLNMRLYTEQEQKIQDLINSKSITISIIALVGIYAILIFVIVALEELMDETEFNNVSIILSWIELGILIVFILEIAVGLYAWGVMKYYKDKWLILDTLIILLSLFFVIFELADQKTSNVVKVIQAVFRFLRIFLLIRKAQTFRRLSTISTISTPAEKIVRFLSELKEVIELESMKLDIDYCIDKIGNNQLYQMGKLDEDNAEAMGWLNQSQQGKSSVRKIVTGDQQQQQKKVLANFQKLNIPKRVLEVLDKQHDDLYIDPFECDKLSGGEGLIYLMLFLFENYNLYDVFLIKPKVVRSYFEEVTKGYQDNPYHNRVHAQDVAQTCNFLLNRCKFIEIGQLDEQDIACVLIAGAIHDYGHPGLTNAFLINSKNELALTYNDQSVLEMFHPSQCFKIAWTNQKANIFENLPFQKYRRIRESIISMVLSTDMAHHASELAITNSRVAAPDFEPYGKDKQRLMDLVVHSSDVSNPTKNFEIYKQWTEKVLTEFWQQGDKERELGLPISYLCNRYTTNMAEAQLGFIDYVVKPTFSCIQGFLPAFEPYFENLDKNKAKWQELVGYYKQQLASIQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZH6</accession>
    <name>A0A8S1KZH6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0310043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8060909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZH6"/>
    <dbReference type="OMA" id="EFKDSAC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="255" mass="28589" checksum="B64064164EEFDEFB" modified="2022-10-12" version="1">MNTSFEQINKSPLNHSVSKQLYSFPKANRFKEFKDSACPNIYTLPSMMSKRGAGIGYGQKSDLIQESITPGPNNYQIKTTLNASNGWTMPVGRDKSNKYDGIFLGLIQKTPGPGQYEFQDSKSPIKYSMRMRTESQKEKDRKPGPGQYNLPEALNENGKYKISKFRDSGAIILSPPKAKSSRFSPAHELTPGPGQYQHTGDIDPKGLYYCSKFADTKSTVFTRAKRELTKIRVESPGPGAYKLPSEFGYYEKPIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1D7</accession>
    <name>A0A8S1L1D7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0320045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1D7"/>
    <dbReference type="OMA" id="HNVNMEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="312" mass="36994" checksum="2C866D05A5DD889E" modified="2022-10-12" version="1">MKPQKSIIKELKTYQQDLYKSGREGKQFSINDFPNLSRKPIEWTDCDQFIKLRQMKCDKKLAIRLCQQYKIHRTDQLFFDTLHNVNMENIGYKKIQSSTSFLLRQIRQNQFIKQKYRMEFQDTQIENKMSSILDDSFQKFQKSLDKHLEIDKDQSHKFNLKQMTKEELYSKLQQLNIIPKQNLSMDHLPSSNSTHRFNLVIPSGNSLHISQTERKFSKPFVPYSSISKNRGSNVSIKQQFTKLQNNQLNIQSFKLIKQNKQGRASFNPQVNVAKEIKSMHHIGHLNDMPELQKQHKRHFTNYDITGNIVNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1P7</accession>
    <name>A0A8S1L1P7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0320111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1P7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="401" mass="46973" checksum="9CDF9D325509D145" modified="2022-10-12" version="1">MDKDIIDIHLFHLKQLHYPSSQLLVCKSSDNELMIINQLGTIVAQYNHTYDILMTDILHNVDEFYIGIYDINRNLTIINMTIIDRNIKEQPIDDIRRIDKNGFYHDIELIWASSFQLNHKATSILVNSGRNTRVFLVGDENGAISYYFQNGTLKNKNTISSTPIMNLLRRHPHIMFQTVKLIGFLNSVSLTVVTPKCDQPAASIVSIYQENIYSQNIYVLLSNQDILIYQIKSGKHHECSISYKIPTLQKIKNIRVINSYLIGYDKTLQIYNTSLYKSLYQDEVLYLTSLDDKRLWYQYYSIRSHKVNALTSNAFEFYTLNNKTTIRQIELTHVPMPQSSSFSLMESFRFPMILVAIFIVFIFQFWQKKKKTDNESDELSAETKKKIDDMVKQYKKYDKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4T4</accession>
    <name>A0A8S1L4T4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHHC U11-48K-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0290151</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4T4"/>
    <dbReference type="OMA" id="GEDMEQH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="4">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8059756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="149" mass="17975" checksum="9A5BA0F20CBCC9FA" modified="2022-10-12" version="1">MQQQQQVCCPFNHLHKMAPNRLIFHISKECQERKMNKHMYRHCPYNFLHIFRGEDMEQHIIICPDKKEPDAPIQLDQNNMENQYLQGDPNWGLKNPNNMAIEIPINNNQVQQDSSLIDQFNNLKLQRQDDIWDQLWKQQNNNEDDFDMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L605</accession>
    <name>A0A8S1L605_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0340356</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L605"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8063480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="406" mass="46950" checksum="3B20810C861F1CB6" modified="2022-10-12" version="1">MFQPKSMKFTPHNSIVGNENINTLNKSKANKNMCCMNHPQKSAKYCMAKDLSVSLCSKCAINMAGKGFIVEEINGFEERQRRDVIDKFLNLVNRNLNQYDKALSGLLTKKQDITKYFEQQVDKVIRFHQLVTQQLSEEKDKLIQQLTQNFKQTSLTYEETIKSLQNQKQESIGMKNDVQQNLDRIVKQIAQEPFNEIMNCYHKTVEKYMSDIEDLQRKPIEVIKIILNEPYKIDEFVTVGLQKTSLIKKNLSDIGLRTITNEDVYESIAKRDSVNNQKTSKTLSSLPRTPKTESSPFKSVPITNIDLYHTPDQQQIKTLKTQTSLQQAKMQLAQVQENIEQHYEEGHSQMIERDDVENSYQMMIAESVSDSEDFIDQLNLIQSLTVGQNGHINTLKMYSSPQFKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L7V1</accession>
    <name>A0A8S1L7V1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0320093</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L7V1"/>
    <dbReference type="OMA" id="MDGEDWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06606">
        <property type="entry name" value="STKc_MAPKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF56">
        <property type="entry name" value="MAPKK KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="63"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8061712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="382" mass="44029" checksum="99030D0DC4720CDA" modified="2022-10-12" version="1">MGLYISRFCKYFRGKESRVYQQQNSGSISQHRSKILVQNNSDGYDSEPEQHFHHTESLVKIQWKKGELIGQGSFGRVYKCMDIKTGRILAVKQIELGYVDKESLESFHQEIKILQQLKHKNIVEYYGCDEDKHHLSILLEYVGGGSITHMMKKFKFNLIEPVIQKYVTDILHGLVYLHNKGIIHRDIKGANIIVDTKGVCKLADFGCSIIGLNAYSLKGTPNWMAPEVINSQETGRYSDIWSLGCTIIEMLTGKPPWGRFQSPMQALLTISSKQCSPPIPINISSNLKDFLDKCLQFDHKKRWKAKQLLQHPFIIFMPKKISKTEQLTPLQKPTEEHNNFDLHVAPQLEDIKKDEEYLDRESYPMILSKRSSGVSDEKHLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9L4</accession>
    <name>A0A8S1L9L4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0340050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8062905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9L4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="82" mass="9499" checksum="E985547F05AE66EB" modified="2022-10-12" version="1">MNQIQIQNRLNSFRATSQRKRNTSQYTQQSQVLVKNKCVQFQKIKQNNLLELLLMDTGTRMPSPISSQISVGIGRIPKDFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBJ8</accession>
    <name>A0A8S1LBJ8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U3 small nucleolar RNA-associated protein 11</fullName>
            <shortName evidence="5">U3 snoRNA-associated protein 11</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0350162</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the UTP11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LBJ8"/>
    <dbReference type="OMA" id="HRKENTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007144">
        <property type="entry name" value="SSU_processome_Utp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03998">
        <property type="entry name" value="Utp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015952">
        <property type="entry name" value="U3snoRNP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="190"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR015952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8063831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="219" mass="25940" checksum="A70F8B35B138FE60" modified="2022-10-12" version="1">MSNFTNAKPQRKYRERAQPTSRQFLGILEKHGDYKKRAINYQRKKEQLQKLQLKAALRNKDEFNFRMLKSKVKDGVVYEDQDESSGDEQEILKQIKTQNQNLLKASIQQKEKLTEKLKEDLAMVQFEQPTHKFFLKEARKKSFDQIEDKLQGEAQQSTPNSELTQKINKLTEALYEKERMKRFYVAITKSKTKDNVTAKPHQRKRIKKGKGKGLFERSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LC31</accession>
    <name>A0A8S1LC31_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0340105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LC31"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="51" mass="6066" checksum="C3DC1067B7D1474E" modified="2022-10-12" version="1">MQYQIKQLQTQSESSAFIMEEGVGEFMFDMISNYMIKIKSKKINNQEKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LD55</accession>
    <name>A0A8S1LD55_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0380222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8066008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LD55"/>
    <dbReference type="OMA" id="ESQMKHP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="357" mass="42749" checksum="4DA139DD2FAE5498" modified="2022-10-12" version="1">MSDIYSNINSQLHTNQNLSLYARHQKEARIEHSKLTPEEQKEKLLQVYNNRPPVFSDEAKVPPKQITATQGSQRQKLQMGIINPIHQFEDPRIIKNEAKQKEQEWYAQRMKQKQRPKPEDPLLEPQPDLVLQANKIQLKPPPMLEKEKPLMQDHLANPYIYIKKIFKKNSVESQMKHPDIWDKSTHVESLRINKVMKQEGKKRPQSAKVIQQQQIPQQDKQKEKLNKKEREEFYLKNLKMKEFLFKFFKTINHLLKARKYNKIIYKGDQQNYDLNTLFNLWADHKKFNFKSNPEKFITKEQFVDFLLLIGLDPDRSHEAERFFDYCHEDVVKAPGITQQEQNTQAQQDSKWFRLFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDG5</accession>
    <name>A0A8S1LDG5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0380283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8066128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDG5"/>
    <dbReference type="OMA" id="DYVSCNM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="26444" checksum="74303C0B6E6CCBEE" modified="2022-10-12" version="1">MNNSFSAEAQSYQVRSVTTGQNIQRTSNGEFQDAVQRYELRRSSKPRQTQNNQQTTVITSKYVQMDNEGRNNNYDLEASRQSQRVLEKYQMNNQNSYYEVNRQSVKEVMDKYKRGRGTTTSQLQTTEVKYSAIPQPQYEQFQQKETVFEGRNSRYVEEQDYSQEKQMTLNYNTIQTNQQVYEVDYEQIKEDCQISDLYRRPQREDDEQVDLLDSDYVSCNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LEH0</accession>
    <name>A0A8S1LEH0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0390103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LEH0"/>
    <dbReference type="OMA" id="QICIRMF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8066468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="131" mass="15577" checksum="8372D1AD34810B3A" modified="2022-10-12" version="1">MNNHSPSRLEQNKSTQESRLNTLRKYYELSLEQRKDLEYFTLIKKFINNQEEYHDTDSSNAFSLSETSNTVYIEGSEQQQICIRMFDIIRQMKRAVKQSQKLCSNKIKKLNKIVEKRHKEINTIKISLGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHG2</accession>
    <name>A0A8S1LHG2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0380008</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHG2"/>
    <dbReference type="OMA" id="ATMPIFN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01801">
        <property type="entry name" value="Ubl_TECR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000131">
        <property type="entry name" value="trans-2,3-enoyl-CoA reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF28">
        <property type="entry name" value="VERY-LONG-CHAIN ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="150"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8065655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="309" mass="36656" checksum="A4B19674BF56256A" modified="2022-10-12" version="1">MSDQIIIKKRKGEYIEQEGKVINVQLNSSGQTILNLKKEIAKITKIKPIRQWLTTEDKQRVFENNELPLNLSGIQNGQTLVVKDLGPQMLWGAVFYIEYLGPILMFFMLYKLGNQENYTFMQKTAYWMVILHFIKRILETKFVHVFSRNSMPLKRAFINCLHYWIFCGFFIGIELFYLRSFSKLQSWKYIFVGFFGLFEFLNLMCHIRLSSFRKKPELKQSDGNYIAQNKQRQIPFGWGFGAVSSANYFWETMAWVSFSLFTCSYAAIAFTIFSFGQMLIWAKQKHKRYIKEFGDRYPRNRKAMVPYII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJQ2</accession>
    <name>A0A8S1LJQ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S59 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0390219</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJQ2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007230">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP98-NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04096">
        <property type="entry name" value="Nucleoporin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51434">
        <property type="entry name" value="NUP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S59" evidence="3">
        <location>
            <begin position="587"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="152"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="728"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="409"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="679"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="730"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8066635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1243" mass="149059" checksum="D084DBF8BEB23AC6" modified="2022-10-12" version="1">MSNTLFQRNGNNNIFQTSQQNSNTWTIPQPQKKRDVQGSYRAKFEQIKIQVVDDKNVLSTSIIFQPQYDKMTMIMIRLEDYYLIRAQQIDDFHISTIQNAIQCKRFNPTLISKVAGQPINIPLFPIQKGTNSIWESNNQTQQQQNIFNTNVSSSLNDNRQQNNFFSSMNNQNNQQQQQQQKQQFGNIFNAPPINNNTNTNNTVNIFSNPRQDQQQQNIFNSNVYNNNKPQSEFNNYQTKDTNLPWTQQQQQSGNIFQQNKPQLQIQIPNQLQTNNNDTNQFNNFNNQNNNNNQSINSIFSQPQQQFMNNNIQQPFSQQQQQNSQQQLMFQQQQFQQQQQQQQIYPLQIPSQPFQPFQNINTINMLSNDLQMIQYQNQMNPIQFIKQSVDMINNFTTTLNNSIQEMEKLYKLEEEKYLEQEYLINQLEQKKRIERQKKQQEINLLPKKKNDSHNLFRMMPSSSNLNLANSQKSSRISIQQQQSQINCGFTTSRISQKSNKTKREYIIEIYFEESENFITFNEEFNDIKSKIDFKEQILQKIDQMHVFKSDKHAYFEKSEFIIDENNNQDNHQTLSFRILQTYRPILTKKGYYTLPDINQLTINQLKNVEDFTIFNEFGSLRWDSPTNLLYLNLDRIVDIQDSKVEVYDLDQIHEYLKPKINKKLNKSCLITLKVPITINKDNYESELKFLQQKCKEQNIELIEIDQNKQQFIVRVDHFSIYTFVHDENDEQQQQSENSKQQQSQQECNNKKENSLEQEFYEKQQTFFQQVYDLQNLSKQSTNQKILKINQTKRQNIQFVQDFQICVSNKEMNEIDYQICQYFMQEYEIQKEIPIENQFCIKLLQCLFSIRNNNQSQLVPYEKRLLGQRLQYFNILFGNSLIQLQDYIDLIEIYPDQYEFSDLNHIQRPNQFQQGLSLILFFESILESRIKDNIQSSYIEKVIKEIKNNSLDQTQKQQQILQFLSSQPTQKIQLPIKIDDWISEILQWKVNQSFVQYSQEWWLGLLNLLEQFEIQSTELASLIYFLSSKFNRSLDKIYNYLNKNNDTMSLFLELLIYNQDKANIKIHKRIITNKISFLIENNENTQFILQILNQFQELFSKSEYQKYKELIFKMQNTLFFVNKFQIIDKQIHLQKLIYQQDFINLGIYILSLKSPIFQEYVQYIFQYTIPILIIFGMNECLQNIIKEIKLKQNIYKLKEREQKILNMSEMFCGTMMFENQVFQNFESKNVFEYQMLQILDEYYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTJ6</accession>
    <name>A0A8S1LTJ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0470137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTJ6"/>
    <dbReference type="OMA" id="QIVIVNY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05467">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048:SF0">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="184" mass="21450" checksum="CDFB73F67CFD00EE" modified="2022-10-12" version="1">MNTQVFFRIYCKTQFSQHVKVVGNEPQLGNWNPSKGLQLLTNESMYPIWFTDYPLELLAYSKLEFKVVIIDDQNCYWECCPNREMNVQSLKQIVIVNYDNPSIQVLGIKTLVSEVELPNIEQNRARKVSIQVQDYMSYTDSDSEDQNSKKSQLTKDNSLFPSIESFNSCLQPNEDLQQDRIDMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LU37</accession>
    <name>A0A8S1LU37_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0460099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LU37"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000415">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="40"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="244"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="617" mass="72651" checksum="D93A4CC7DFBD4BFC" modified="2022-10-12" version="1">MDKSTVNVRSSINFLNNNDQEQREKIEIQLIPALQSFQSFIKMTNESQMAQIVDSLMAIDFFKKSCPEGSSLRDFIMHAGKALTYEYYKQGQIIFHYGDYGDKFFMILKGNVGVLVPKGNHDIEMDRDFLFETNKNEPWSYAWKGRKLQSTIDMELLLAQGEDPWVLSNRYFEGGVCLYQKIYTYYGGQTFGDVSLYTDKPRTASILVLSEDIHVITMNKSEYKQICEKSLQDMNNNVEYFMRMLPNNSKFVITKFMQYMHKIEFAPQSILWKEGEESKFFMLIFKGRVELIKKIGKVRITLCQLSDNSWVGQEEIFDQSIRQSTCQCADNTVAYYIQIDEFNQIRRNFPEICKILKERSHILKEYMKIRYEMIMNNFINKPKLIQKERQKVERKSITEIFHTSIKTKTQDIRSHSPKSLSIIEITEYNNKINQSRGGFRNLNIFPLDKDSVLSIRDRVCRQLTKQNKKKQRVQPQTMREGTLLSSTISDLFQQNDQVKQHSLYKQNDLKKNSFSFLNLFAQGQSNNIPTNNSDHFTSRLTTQQSYFRMKTEQGRSQIQTQTSFDQKNNSVELNKLRSNSPLNSIQFSDRHQNKRIHSFKMIARPKKYPQLLKYKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LV09</accession>
    <name>A0A8S1LV09_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0440272</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LV09"/>
    <dbReference type="OMA" id="DHANYMG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051218">
        <property type="entry name" value="Sec_MonoDiacylglyc_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856:SF25">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035849193" description="Fungal lipase-type domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="87"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8069855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="289" mass="33177" checksum="592C23883564DCE0" modified="2022-10-12" version="1">MMKILLISLVIVLSYQTVEYNTYDEAFAKHLFYLTSASYCSQQHIKNWNCGKPCKELKPITDISFFINATNDNAGYGAYHPETDEIYLVFRGTLPWSLTNWIEDIDFIKTDYPYCSNNCQVHRGFYYSFLGIQDQVLNCLKTLTKKYPLAKITVTGHSLGGALAHHALVYLATRGFTISKFYTFGSPRVGDKNFFTYVNQQLFPGPKFRVTHNHDPVPHLPALIQGFHHVNQEAYYKDYLLVIHKKVQLCNDNLKEDDNCSNANLVDLSVSDHANYMGYDMTLELLTCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LWR5</accession>
    <name>A0A8S1LWR5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0450009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LWR5"/>
    <dbReference type="OMA" id="EDNYEAR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="2">
        <location>
            <begin position="37"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8070003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="136" mass="15166" checksum="A8C6BFC752D2AB94" modified="2022-10-12" version="1">MTEAEQTFYVSPIANPIANSKLQKGLLRFIARQITIKHIKRGVKEVGKALRKKTPGIVVFAADISPVDVLSHLPIQCEELGIPYIYVRSRLELGAAAQTKKPTSVVLVQVPTKDDVKGLEKYHELFDKVKESNPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3I8</accession>
    <name>A0A8S1M3I8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0490096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3I8"/>
    <dbReference type="OMA" id="DEECVPI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="247" mass="29645" checksum="11DC7971B8D73F12" modified="2022-10-12" version="1">MHPHYFPYKFTQYHYQTPPYLYPQYLQPQYYNQNNFLQYKHPQFQYAPLPTQQQQYILISDDEECVPIQKIDKPIPTQTINTTQTQKKTVKLLDLDTLEAQGKLYDYESSQSPPLPKRIIKKPPKPIYKPQRRKQIKIRKEPIRHTQQKIKHVQIGIQRQQLNYPQSNVKAKFIKIYEKQEDNFSKLFDQLKLNFSEANDEDIAIVLNMVGKDYQKAETFINQNSFFLQSYLQNYKDISEDDSTIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8D5</accession>
    <name>A0A8S1M8D5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0550208</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8076049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8D5"/>
    <dbReference type="OMA" id="DIPMKSH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000121">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="282"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8076049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="477" mass="55151" checksum="03DFE7C2D26AB25C" modified="2022-10-12" version="1">MQKTNIKSVTARNEQAKAKLTKEQFLTLAKMCQSSSTQQLLNKKLPSQQKPMLANQLNITPRNNTPKSCRTAQEMKYFLYQKNLVKDSINKPQNHSQLLPKTKNSLHRNFSQLEKSSNSQQNNGQSTAPNTVKKKYNLLSTDSANKLDYRFSSVQFAQQIAQQLKKKFEPQQPQQKKENLHVSLDDFVTQINRPQSIISPFKQTKSLSPSTRVNIVPSKKSLYYVSSLVQSFRHILPQNQEQQIFRDHAVQTFNCVSFCVKLQEPTQMILEQKRMEIPMKSNYKFKKTVVFDLDETLIHCNENQNLKADVYLPITFPSGDTAQAGINIRPYAKWILQELSQLCEVIVFTASHQCYASQVIKYLDPHTTLLSGQLFRDRCVLSQDGVHIKDLRVLNRDLKDIVLIDNAAYSFGVHLENGIPIIPYYDNKEDKELKMLYDFLVDQVLPAPDCRIVLQSVFRLREYYNYGEPKQAIEKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MA99</accession>
    <name>A0A8S1MA99_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0510082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MA99"/>
    <dbReference type="OMA" id="EDQLNYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="61" mass="7433" checksum="86D36848D10265C8" modified="2022-10-12" version="1">MNQGNQSQQQVNNYHNPLEDQLNYKNYYRIMVLLFAILVILGIMQTYQFTQYKREKLLTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEL7</accession>
    <name>A0A8S1MEL7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0590089</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEL7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="167"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8077949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="254" mass="29351" checksum="4025D1F514608607" modified="2022-10-12" version="1">MEDIQLIIRRIKRSGEPRLNLSGKQMISIPPEIYQLKLVHLDLSYNKITSIEPRILQLTTLEELDLSKNCIEEIPEELLSMSNLQSLNLTDNPLITKFQILNGHFHQPQLNQILQKLFQISPGSYQQQPKQEQKIIPERPQTQSRAIRQIQELQHTNHVLEVESNEFEVHEIISQGGFSIVHRGYFRGTEIAIKKIFNPNITQQLLDEINNEIEMLSLLRHPNIVLLMACCTKPPNLVIATEFIQGGIIKFQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MF38</accession>
    <name>A0A8S1MF38_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lipocalin/cytosolic fatty-acid binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0610204</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MF38"/>
    <dbReference type="OMA" id="YCTTATY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19438">
        <property type="entry name" value="lipocalin_Blc-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000027">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047202">
        <property type="entry name" value="Lipocalin_Blc-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612:SF34">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08212">
        <property type="entry name" value="Lipocalin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035738248" description="Lipocalin/cytosolic fatty-acid binding domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="2">
        <location>
            <begin position="23"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8079240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="184" mass="21280" checksum="97A45EEC0C8B116D" modified="2022-10-12" version="1">MQKIVLIFTIVTLSLAIDAVDDFDVESYLGDWFEVASSPWVYTTQEKNGFCTRARYGTLPSGDISVYNIQRYGAGDGPIRFIEGYARIPDLRQKGKLKVYLNKGEVVGGDYWIIELGPLINKQYQWAIVSEPEMLLMWVLSRNPQQYREEYEDYVRDRVSALGFNGVLNQYVPRFFDKCVPYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MGM3</accession>
    <name>A0A8S1MGM3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0620202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MGM3"/>
    <dbReference type="OMA" id="NYMWNLE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="61" mass="7204" checksum="67C06156F75437C7" modified="2022-10-12" version="1">MDFILLIQRKVSQKNNYMLNLESLDKLISQNCIDILLLESQSILLSYISHLKKKLVKQKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ML45</accession>
    <name>A0A8S1ML45_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0580172</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ML45"/>
    <dbReference type="OMA" id="SKEPWAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="636"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="542"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="551"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8077556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="714" mass="84770" checksum="5A56E705590FAE85" modified="2022-10-12" version="1">MDCEAVYEILQPGFIFEVIVNKKNIVKGRLDVILQKFLAFSDKCITSQHQVRSADFYQFKKQKHLLSQLTQINVMRMKGVIYEQDVIEDFSIFTYYINFEKIQVGDHLIAVLIDKHKMTMSLKNKSIMGGKQWSLKSLQNPSDFDNWLYFRLGYAKQIGVNFQYQSMNEEEKKKAQEQINANLKFKEETGILKMDYQIEQDGFFPNKQNYPFMMQSLGLSQNTSFTNRNNLKSFMKINLDHRRRLYQIIYFFQTFKSIRLARCPIDLCSQIEESKQQLKNNKITILISINSSYQYNLLNCNQINIFYQFIMLPKFQNSFALDKNLQTLIESFNQSQKNPGAISRNRIQNNYPCIPSVIRQPPGSQTLNLFLEKVTFILLSNSAINTTFLNDQQDIILKLLFNNKILQLVILDRPLREQKEDDQSFNLYQQSVYPFQQQHKLKVDFKLEDSDEIEVNGDDNFVTILTKAQIKQQKNEQNKKQIQCTRLKLKLFKFNSNFQENGIQNQEIIFLLLKKLSKNILNPYQYPKSNLQHLIIKPSTEQPIIPPQQDLNKKQSIKTEVKQEPEKEQEGVEQPPQIPEKIFKTYFLQQVKSLYRDKEKHQTKNCTSEEKIKRLFEEVRIKQFKQQPYSVDKNFFKCPYHRCQKGYKRPNQLKNHLKQNHQKLSEIGFTIDDNGEFKYNERILDYCLLLWKVYPNFVKSVINEMRQRKEQPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLS0</accession>
    <name>A0A8S1MLS0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040112</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0650226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLS0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="450" mass="52621" checksum="1F72A6309554CCA5" modified="2022-10-12" version="1">MILKNKIINSAVVITGQSLEILNQNEKVAFKFDCYQSSAIKSQLLSVQEFQRISLDQIKIVNQLRIDLSIISIQSNSKIMLNTNDYVIIHNMSFIGNILLKQSQTMYFSLIDIYSEITQIVTLEIIIYYYNIFDQYQIDPSQNSASLMQINAGQSNLSLKNIISSHNVLTNSLYTFISISIHELYIQNFQIINHIYLNADLWNQYYQLQIQSEPTQSQIISIINNIFKIESLSGVLLIKTSKLNFINGFFSNIIASSSLIFNINLQGDGIMMKQLLLMLKKSLLSLVIENLNLKYIQNRISSSIFSIYPSSIKNNIQLKQIYWYNSFSLYNQFISLEFNFNNPQKPIFNINLLIIQSENEFINYLQFLGKVSIFSKSKIFQKTKQQYIQLEDSFLLRESQLKDQYYLKLSKQLIINQSKLQILNLLIYQHFIHRIQQNFLKLMKFNQCQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPF4</accession>
    <name>A0A8S1MPF4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0670094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPF4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF10">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8082120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="268" mass="31839" checksum="B7BDF0AFA1EAF722" modified="2022-10-12" version="1">MLAKDKQWQYRKIQAVMSKWINEADILIEMIICNFPSSIEVQKNRTSYLFECPLNNTIPQFMRECDFKGPLICIIQLNLALLNLQGVFQQISQSYQEQILKKVDFFFWQNIFRYCKKKLFKQNTDDIQLSIIHIRCFLWLYFMIRMLSYQGGVQKNNYLIYIGSPRILSNQINELFSFTINKSAQCLKRLFQSDQLTNCKSEKTRVLTLAGCGELHLDICLNYLVNDFAKFKQFDLIQFYLIKKLFKQHKMLSLLLNLSIISYKICLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTR6</accession>
    <name>A0A8S1MTR6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0700208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTR6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="87" mass="10490" checksum="CA56818028900182" modified="2022-10-12" version="1">MPYFQIQIQIVNLSFKLDTMNNRNNVIDVETHVKHVQIKQYVKNVFCQSEKQLNYLFFLFFLKYMIILINQVKKQIKYSSPQSISNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MY68</accession>
    <name>A0A8S1MY68_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0670213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MY68"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="27330" checksum="18CAC7F5F5602631" modified="2022-10-12" version="1">MIISNMTQNISRVSDKTLTETNLIVYSLNKFKRSSLTNRNIQLNKEDLIQSDETQLRRKSCYCSECGQLSQFQYRHLNNNVSIKKIKIFSKNSMMSKSMKIIPTSNRQSILIQRHQFRMNTCREIQLSHVYNKSPAARLTPMKENTITINKIKEQYYNELLEQMKKKNRLNFHQLSLPQTKRDQELLQNKKNIQTPQIHRKQKPDIRPYFSHLKLKPINQKFQTSHFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0B5</accession>
    <name>A0A8S1N0B5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0760039</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0B5"/>
    <dbReference type="OMA" id="LDYSYDW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="11"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="348"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="348"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8086168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="469" mass="54064" checksum="3DC17108668736DB" modified="2022-10-12" version="1">MQTASIFNGKYVAQKKISSGSFGVVLLGHDKDKNIDVAIKIEKEENEDVRSLEREVQVLERLNGIEGVPKLYWHGEQDDFNVIVMQILGKDLAHYVKSKKKFSFKTTIQLGIQIVKVLERIHNKGVVHRDLKPENVLFGIDDESSKIYVVDFGISKIFRDKNGNIHPFRDNTSFIGTTRYASIAAHKGFELSRKDDIESLIYVLLYFMKGQLPWQNMQNVSDEERTVKVGEMKMSIDLRELCKDVPIEFAFILEYLKQLQYLSEPNYDFVVQQFEKAAENLCIQLDYSYDWDQQQQPMKISQSVHSEIMQRSTTLNQQLQPPSTGEMKKSLDKQGSNLIRQMSNNQFLAPPQSNQLQPPTLKRQDSSQQPSLVSGSVMLSTYNSIRPKYQPSQLGLDVKVSIDTVPKEYIQEQRKTSESAQQKKAQNSYTETAGWRDSIFEENYDEIPLSKKYGMLQQKGVDMQKYICY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N442</accession>
    <name>A0A8S1N442_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0720108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N442"/>
    <dbReference type="OMA" id="TTDHDKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="150" mass="16786" checksum="D29171B3508690DA" modified="2022-10-12" version="1">MGLDLKMRILLSNTFMSLVVVVNLALVSYLKENDGGDYTQFSYITNISTADHDKYRNYLNLAISAFVLMLVGFLIGMVQPFLKQAALAYVMALLILAGYVCLFAGVFINDTFQYNFSDGKSFNYSLASFFGMGGVFIQGMVNSWRQRSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5J3</accession>
    <name>A0A8S1N5J3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0760170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5J3"/>
    <dbReference type="OMA" id="PYFENQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="354" mass="41502" checksum="83BFF9180C24503C" modified="2022-10-12" version="1">MHLEIHPQNSNYRQNQAGLQSKRETSSKLSQHQSPIIPICLKQSSNRENEVPQEFDGEKSIYKSFIDEYYSVRSNFPNSPSVKFQKKKIFDDIQIPSQKTSQIKTNQILNEKSQTNNQEIYHTYQQLSQRDATLIQQHPYFENQIKSLQIDSQQQFKLECLKDSNKENQIVFKNKQILITLKRISLIKNILNCELTFQNIGNIPISVLDVLYSNDQTLRLSKENDCIKSLTPGYGQISTITIEIVNVPFTIVNVSLEYNNQFFKTYLPITILNFIQDYQTTQSRCVNNHNHYNKLIINKKNWNIESRIIIGNILNSEVEIRVENQNNQLVLYCQSDLEQLNWQILNTLYVLFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6M6</accession>
    <name>A0A8S1N6M6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0810091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="DFLNIMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1137"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1315"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1426"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1625"/>
            <end position="1643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1689" mass="202391" checksum="4DD733A757904964" modified="2022-10-12" version="1">MMEHLLVNFNIGKLKRPSYSYSIVLITITFCQLISGIFSKYYLIITYHYPNLQFSQSSQIISLIKYLDINSVIQENEIIIQILYWWLSTQNIVTYTLYGICYYQVKYQNKKNLMDQSSAKMLTQLLLIDQWITCQPTQLVYLNIVYCSTNLNCLNIAINTQIQQVLASCFLFIRFISQNIMILFFINHHCIKKDALSSGSRFILLYLEVFRILLNIQIAFPDMSQEFHVIFLIYLVLSTCIACFLFKINDFKLFISNRNVRQVFKIFIFSLFGISIGLTINSISIHFNITQNDNSFSISIVSVILIIWIFYQFYFNQQIQHICQPALIGDDLIQKIFNLKSIAKQIHINHEFGQADSFYLGIIFQHFTNQCPQEKKERCFCMMRSLYDPKKLKDIKNSQFSMLRQKTLYLKYLIKTWYEIYLFNHPQDVNIRMNYAVFLYYQMNNQVQSLIQIKLTRTYKLNLLQQFDLSKIEMQFQNEIIDNNSLSYQNELDFEYVIKIEQIIKSIEFNIRNFLDFSIKFWRQVKQKVIYEQDLWNLNQQIILTIQKSDELWNQINKYKIINRDKLIIKNFLIRRPKWQFIYNWYYLYVLNKKIKQSMLEYCDSQNMMQQPVDEDSGSDNQNQIDQFSYLKPFNYTSAIFHTTQSGQIVNVSESAYDIFGFQQYNNINQLLPQTIIRNHQYGIEQFVQTGKSRSFYKKRKILALNNEKYLMPCKKYLKWHISPLSQIEYICMIRPIYRHQSVNYILVNNDWEIDCVSEQISEFLQPGLCLFLLCPKLLKYSYYSQFLTEEDLQLFKLKRTKNEDTNQDSIIDAKFTNRKITIVPTNYKNQYVQQQQTQDLVSMVLEDDTKNVEESPLWINDALHEEQQEEKDQLFRRFHKVVDEDHVKLTFRLPKKIKHLIDDYAEIKQEIFLNGLDQMTIKSNKLKDQKIVKRNSAGKLIFDKRMLFSKLFQYQEYYYETLYNHFKNSFQKYCMTSRMLKTVIKIEASIRFTKNTIMDKYQIIKITNLNIIESQALDKKAKVVKLLNRQNQVVRQRQSVFQNHQSQHLQFFLEQNFQQQIEQNYQKLLHSNVQNSEVESYRNTTNRAFMSEDQVISDFNTQKITSLSLKTQNNIFIKEENNKPAKQDAQLNFFKILNRIFIFFLIIFNLIVLYQGPNVNQFELDELGLQSAMKQYDFLNIMIESYDKLLNAFYCQNNILSQITNQSDFYQDLQNSFNNQLSEIKSLYQQSNFVQKVYKSKNYDNNQSTIDLINLYTSNIGNLQLIDQNYDFDQQLDFFRTIFIPFLFNYQSRNIFQIIDKLIQTYQTQDNICFTIIMTSTAIFGFYLIYNIFNILKLIIQCQKVAKQLSYIEKTQVEESIKFYLNLKFQFSCISSQSGLIQNNYFQSTNQTIIKQMDEEEKSSNLKRQKQKQYELSKRWKRIKVLMIIKYLFFMSLISFLSYFYFIHIMRFNNLMLNLLNSNYFTKETTQLLSLTISKELFIYDFFNQTYIDIQNYKQIQSRYINNNAELQSDLYNTNIDEIDEIFNGNLCIATINSIVNFQYCDTYLNGALKYGLKSYNFLLSQMAQQLLNPEEGLYENTTIEKIFEFDQVNKFQRVGYNQAWKILGQRYSQYIDESAQLEIIYLSCSFTFTLVLFIVLFEISFFDQMLQIILEVRCFYKRYFSNNTIDRYKIIRAQLIQCKIINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N966</accession>
    <name>A0A8S1N966_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0830087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N966"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="57" mass="6351" checksum="5ED05A628478D477" modified="2022-10-12" version="1">MVNIKMAQRLVDGIQFGFGKRGLKISGGDYIKLMILTTQQRLGSGQRQLLILDSIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGC7</accession>
    <name>A0A8S1NGC7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0830147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGC7"/>
    <dbReference type="OMA" id="MFRQNTS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="221" mass="26040" checksum="8C37EF27FD80A33A" modified="2022-10-12" version="1">MTDQPYLSLSKVKSSHSHHGYHKKLVLQLDEDEQQHMPMFRQNTSKSRHLNNGTLSKSKIQNIAQLQEMFKQFKHFKCSLQTQTPAILMMKSSQISKKPQSQSKSTLDAKEVSFEDKQKVMIQARKTMFRIVMDNKLRSQSIKDYGFNLRPLFNQVIEKNDSVLEQKIQEQRQEKRKSDLFSRSPFSNAYYATKALSKRSRMNVYERLAQNLPADSRFHYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHT1</accession>
    <name>A0A8S1NHT1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0820192</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8089023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NHT1"/>
    <dbReference type="OMA" id="AHETGKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46368">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46368:SF4">
        <property type="entry name" value="OS10G0403700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="3">
        <location>
            <begin position="141"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8089023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="39849" checksum="0543E7841C1109F7" modified="2022-10-12" version="1">MNNKVRIGIMSTAEIAHKVCLAINCSEQAEVYCVASRSLEKARQWADAHNIKIAYGTYDELLDDPNVDGVYIPLPTSLKKEWAIKAANKKKHVLVEKPLPGADSSQCLEEMIKCCEDNGVQFLDGTMWLHSLRTQIVKQKKQELGKISKVIAAFTFKAPNEEWLHGGNGRTNKNQEPQGCLGDQGWYPVGAILFAFDYELPQFVQCLQYKLNKVDTIIEYSGFIEFSNQRYAYFDCGVTSPHRSFVDIVCENGQIRIDDLVGGFGRTGNFNAYFENFVGSERFFVDDLQGKEEVIKVEGSNHTVKMIDTFANIIKSKKNDHQWHHRSLTTHKVLTALFKATQKVGEKVQILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NNZ6</accession>
    <name>A0A8S1NNZ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0970142</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8095007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NNZ6"/>
    <dbReference type="OMA" id="QKETKTW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000334">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF75">
        <property type="entry name" value="CENTROMERE-ASSOCIATED PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="5">
        <location>
            <begin position="22"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="340"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="646"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="95"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8095007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="702" mass="82329" checksum="A90881188D685646" modified="2022-10-12" version="1">MSNMKGIEQQTKSKYHESHVENVLVGLRLRPTDSNDKICNIINEKSVIVKKSNERFSFDNVFDEAASSKDIFTKMVQPIVQKCLLGYNGSICAYGQTSSGKTYTMKGSNKEPGLIQLSIDYLLQSINKITDTYCNLKISYIEIYNENIYDLLIRDLQEMKPNTSNEYKWETINSLNQFEEIFKLGEEQRHFGETKMNDNSSRSHVILQIAIEARQKCPPFIIRNSYLHLVDLAGSEGLSKTQAEGLRRKEGFLINKSLLALQNVVSKLKENKKDQFVNFRDSKLTKILKPSLNGNSLTVMLLTISQNNENYLESVNTMRFGMSAGALKNTVNINEIQECNNFQSEAYNEMIEQVENYQNQLQEIQQQLEFYKNQSQEEQNTIKQIKELVKEKNILIEDQLKTITEQIVEKQRLQEQIHQLQRCNQELISTSNENSNYLNYFRNIVETKGVLNPKLMVECITQTDSCEADQQIKVLEQENVSLKQQISEMKNKFSFEIQDVKDMVSGIKKNKEFTPAKKLKFQSNKDLNVQNENQKETKTWLQKSLQKTNQYLESLNLLTTLQTQLIEQTKQNMILRNFLDKKEQEYTELERANFVLHKSIFDLQEKLSEIQNDDKKENVWKMNSEKSNKQESLIKKRQRLYQSPTKDELKRNLFRQEQRNQIYEKELNKYMKNLDQISKQKQELQQFSHHLYMACIENNIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPD4</accession>
    <name>A0A8S1NPD4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0810107</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPD4"/>
    <dbReference type="OMA" id="LTYRSMT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF36">
        <property type="entry name" value="KINESIN HEAVY CHAIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3 4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="7">
        <location>
            <begin position="80"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="599"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="602"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="618"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="147"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8088504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="638" mass="74018" checksum="414E009617ED0AAE" modified="2022-10-12" version="1">MSYNSKTIDLILKAQEPNQKQFTTFQQFFKSNNPPPLLFEKNITPKNVKAQRSFSNKTQDSIKETKRKSLSQLKYPEYRMLKIYSRIKPGECQLNYDKQSILIETNSYNFDRVFEQSATQADIFDIMAKQTVDDFLHNINGCLIAYGQTGSGKTFTMFGESSNPGIVLRTFTHLFLRNKETIYVSILEIYKDHVYDLVSGVQDLKLKEDLQLGFYVDGLKKIKVDKLESCQELLQIAEENRHVAETKLNALSSRSHLILTIQMGKTKLHLVDLAGSEKVNKTGAIGETLQEAKKINYSLSCLGHVIQCLSQGQDHIPYRDSKLTKLLMDSLQADCRTSIIVTISPENKNQDETISSLKFAQRARFIKKEIRIATQKKTYRDLELEITQLKQELLETKQRMSQTSSVSQFQCRYTTDSEIPCTESLRLTYRSMTNENKENCLPNLKTQENNIQLELENKINQILIELQTLNKEQNFDNVKRQLNKINELTIEIKKTYKVLVSTQGLNNNHLHIQSQFIETQQEQNKQLQKIAEQISNMDSSKPSFNIAINNMIKNLQQQQKQNIDDMQSKLEKMYYDIKKTTTVQDYNLNHLALKLVASPNRQQQQQQQQQQQQQQQQIKRSQSKTNMNPSVKYLKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTQ7</accession>
    <name>A0A8S1NTQ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0930199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTQ7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="197" mass="23468" checksum="B96840F08E55CEAA" modified="2022-10-12" version="1">MYENNFATNSIYLLTFNIINFLLLQFEIGQIQDYQKCRSKKNRSRNIKRAMLDEKRHRKIEREKKQERRNGLGGLKSKLAVAVAVPWMDKLSFDIKRYQNSYKIKGVYLQFPLLETLEQKTQRADQVSKFIIYNKNSLINKNQIPNIFRGKVKPNKKLTNSNNYHNQINNNNNKISKKTTTQTKIQKKYQTLNQIMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTX1</accession>
    <name>A0A8S1NTX1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0940028</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTX1"/>
    <dbReference type="OMA" id="CFAVIGG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15848">
        <property type="entry name" value="SNARE_syntaxin1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF307">
        <property type="entry name" value="PROTEIN SSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="197"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="228"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8093731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="297" mass="34407" checksum="46D2470DE1631BAB" modified="2022-10-12" version="1">MNDLYYQLKETAQQNGITIELIPSNRQSIAEMMPDFQKKSSMIRMKLQLIKNNNEEISRLTEVIQTSASTQKEKESMKKVEQLQVQINKNTKEIKKLFEEIDKLVKEGDPDEPETRAMIYNQKAIQQEVASILQSGQNTFFNYNKTIQKKMKRQLEILEPNLTEQQKTDIISDPQGLEKMVLKETLGQPSLQLVYRVQDIQDKFQDIQKLEKSTQYCFQMLSEIAFLVNQQGEQIESIEQNLNKAKNYIEKGEKKLAKEQEIHKKSRKKMCCIILIGLIAIGVITTPIVLKFVKSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUN3</accession>
    <name>A0A8S1NUN3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translation initiation factor IF2/IF5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0860188</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NUN3"/>
    <dbReference type="OMA" id="VKHHKSR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="4">
        <location>
            <begin position="59"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8090744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="183" mass="21141" checksum="72D64C2C9946AC88" modified="2022-10-12" version="1">MDIIDFNKPNTVKKVKVVKKVVTQAPVVNKQQQNKEYYNKALERIIQLLKENNPSLATQTNIQLEKPQIDKLGTKKTLWKNFEAICTQMNREAQHVSQYFLIELGTEGSQADEKLIIKGRYTSTQIETLIKKYLYEYVLCSLCKSSDTTLIRDVESRLYSKECKNCKATKTVATLKISSKRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXD2</accession>
    <name>A0A8S1NXD2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1030084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NXD2"/>
    <dbReference type="OMA" id="YLFEWIW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8097152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="42383" checksum="74A314D596EB82BC" modified="2022-10-12" version="1">MELQLIKKEMFFLLLYGVIFLALSLLMIGLFEYNGMFYLFEWIWLWFFGMILSMVFLIKEESINEEEREQLLEKEISEHKQTYIPLTLYAIHASIQAADILLICFELFYMSFGLFIICQGIVFVFYIIYTNKYDQLKIIYAGVVLLTGIFSLALDIDGNDKCYIGLIATLLQIGTNIYAIKLKEEFMHQYVFQHSKYISYTGLFKFIIWTIAMVIVNFIPCPQNTKSHKNKCPSNEKLGYFSGYFESTFSEEYNKNQGYIIAYPVIVFVVLILISSAISYIDNKITIKEQQDKKQLIQQFVFFPLWIWLYNINNSVTLRVFCILFFILQWLLGLYIIFYDKKTVKYEQIEEDQDSEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P3K9</accession>
    <name>A0A8S1P3K9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein Abitram</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1040129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P3K9"/>
    <dbReference type="OMA" id="YIIEMNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039169">
        <property type="entry name" value="Abitram"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13651">
        <property type="entry name" value="PROTEIN ABITRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13651:SF0">
        <property type="entry name" value="PROTEIN ABITRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="18092" checksum="2EA39A2BE3D1952F" modified="2022-10-12" version="1">MQQIEEQQQQQGQEQDEDLMIPLNVQARLSEYTDRYFVKSYSEHQYVFQHSNRLYLCGINKNHPIVKSDSDIKEIEFTQKEQLVKGKKKKGGLKVKSNTVMLKVHLENGEVYEVQAGQDGYIIEMNENLQKNKQYLKQYPETKGFVCIIQGCRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P683</accession>
    <name>A0A8S1P683_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1070098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P683"/>
    <dbReference type="OMA" id="NILWGIP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051942">
        <property type="entry name" value="DENN_domain_containing_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288:SF0">
        <property type="entry name" value="UDENN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="UDENN" evidence="2">
        <location>
            <begin position="391"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8098552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="813" mass="94659" checksum="46B868EB8A58DD06" modified="2022-10-12" version="1">MGICYSHDTRICSEEFQSIPLPNLQQEFTDEFTQQENQVTVSQSKKDFDADLPIQTLQNNLNSHPLSKTLYIIYNKCRIERPIEVEQGTYQGQWFAGKQCGFGRMYYKNKDYYEGFWLLGRKHHMGIFIQMNQQPLIGEWFDDKFLGNDISNNLTKDSKLAYSNLLNLQPIQIKDQLYYGEYTNNGKQGFGLCIQKGEYKFIGYFSNDKKQGYGIEITKSDVYRGCFKDGLRCGIGHLKKNKNLFIVILINICFFIMDHLSQRQLLQLLYEKDCYIQALEQQLEFSRQEVSLLKSQKQSIIIQNLSSDEDCNLDENSEPAISDSRVEQFQSSGKKIKLKAPLQESNNFNVSQFRQSTQYLKQKVLDSFDIQNVTIDNLKVIRNNDQLFDDLYILGLGLELSNDPQVIYSYNQVKNQQYILQISKFIFPDGCKPKVIPLSFSLEDINLMMNQNYSIDSSNCFVILTKQTEQQQLKYHICYRYTDFTILSNEQVRFSKRAICLTTSKYLIDFYQQILIAILSQLKCMRNNLYLRTKSISQIDTNFFDKNIAQISFRILSQLTQKNSLNFITINNQELKICENDNILWGIPQLFKKLNSQSLIKIFVSCLIEKSIVFVSKSPQLSTAACLLCQKYLLKPFAWIHPIISNLPLENIVYLGSPVPIIAGLECNFTTLQSQGVINKFQNTIFINLDSKQQIQFGNTDAIPLLSAELMTYLLGRLDSYFQIVQQNHNNYSQCLQIFNQLFHARVIRNIPIEPIRQNTNQTSKKSTKLILDYDKIALKTLQNMGTINADKVIWKQFFQTQIFIQFIDQYYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8U9</accession>
    <name>A0A8S1P8U9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1100033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8U9"/>
    <dbReference type="OMA" id="KTILIQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="205"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="338"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="460"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="631"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="960" mass="115019" checksum="3ACC42E7D4DCC1AA" modified="2022-10-12" version="1">MDIKLQVDNLIKPIKEILQNIDQVKSQIIQSHIHQFALSILEFVFLGNCCPCTLTPVEYKTILIQYIQQLRKFYQNPKESTICQIVSKLIKQRQDHGDREQEGFMNSDEEETRLVKQISFQQNTQDYSNDANLEKHKVILQQKDYEIANLKAKLQIKEQKLLDLASGYLKDLQNMREQMYKKSDDFDYYEVSYCDVTDIEDPRYRDLVNNKIANLKSQYEKKTREIYLFVQKQKSEVQNLRQIVGNFEQKQKMMENVEYLMNRIFSLEQDPYKIWKYIQDIKGNDYFHDVFEKQKPASGIRYREVNKLLMVTKAYDRELEYFKKGIEDQMYVYLERACGIINLQNQDLLDEIEQLKREVERIKQFENRVNQQIMQQFDQFKKQLKQYQEEYLDIKSKELQQLQKIQLKYATRLWYIVSQIKKGKDIKELILYAYQNDYEKLTSQQFLELENNYLKNMIAIKTKQEEIIELNNKLEQIQLSNYELKSQNSQMKVTIEDLKAKVKNHQKCLQQTIKHIDSKINPEQLNLEDNNRETTISFKDKLKQLRQQHNGLKFIQEVNEQFEKNNKALEFLSVYYENKSTQTMICNVNKSLTEAIEIYTMQNNDENINEDQGYFKKDSNNVECQTDLIDIDELLKQITGLKQQIKHLSQDIDDIEGEGTIQKHFKLSNQRNQSSDVYSSQKSANLKRRYVQVNESKDSNSNYQDELRVSNDKNYGYQLGQTNQLIILHQQQSVEKIAQTQTLQTSKLQQKGVFNRLFEDSKKKCERIQQIKKNLELLEKEHWQQVGELVKEDQEIMNAFKDSLSKSKHFYQKQLKQNLEIEQKYFPQKHNRAQFYFKKFKDFHIHQQELNEIYFDPNSSDEKTTEIQRLNIKSVVDNRKLNGQIKFNGLKREEYQFFQQLERRKLQSRTFRSTQQSPKQKKVQYVSFTKPFIASLTSYETNDFNNQQITKHLQKAYDLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PC43</accession>
    <name>A0A8S1PC43_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1120181</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PC43"/>
    <dbReference type="OMA" id="ITVTHIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF137">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="21"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8100569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="318" mass="35770" checksum="DF193E5C3A5387EF" modified="2022-10-12" version="1">MDYVKSAIITLEIQQPRFAPIYYILSSSLLFSINSMFSKLISNIPSSQIVYFGAIIMCLINTIVTSNQKISLYGFTTDIYKKLFMRSLLGYLGTIFFYQGIKQVSISEGQVLFRTSPLWTSLMAIYYLKTEKLERNLFVNLILGFLGIFLISQPSILLEMMGYEKKEKHIETSLIGIILMISAAICSSIIQVLINTLAKSVNQLVILQYFCIITIVATSTNLFIFPSKLWVIPNNFETIMLIMIGVTSYITQLLMNRAYIKGNLAEISMLGQSQVIFGYIIDLLMGTNMTVFSNLGTCLIVASLVKVVQDKQKAQKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PC81</accession>
    <name>A0A8S1PC81_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1120049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PC81"/>
    <dbReference type="OMA" id="MLVIWAA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022127">
        <property type="entry name" value="STIMATE/YPL162C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735:SF1">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12400">
        <property type="entry name" value="STIMATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="35120" checksum="E8B520839DEE2D5D" modified="2022-10-12" version="1">MSTEEPEAQGCELLGGFGILVQSILGFLCFLVLVFKRHIEKPKRVWKIFLMDTAKQFVSAVVQHFLNIGLALLLSSADSGDQCQWYFITYMLDCTIGMLINCLLITLFEKLFQKLGQTQFQTGNYYTISYPQITTYSISSNDLTQAQKAKPKVEVAYGVYVFQLTLWILIVVVSKVILYFFQLILGEYLLTAVSWMFQPINQYPELELVIVLVIIPLIFNALCFWVQDSFLKKSKFKKKEKNAVLDALYEKTDQPVEVEIIDGDESCRPSITGIELQSQQKNNDLVITETKYDVEVNEQQTDSSNEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PDL4</accession>
    <name>A0A8S1PDL4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1140076</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8101034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PDL4"/>
    <dbReference type="OMA" id="RWIEDTA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="18"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8101034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="38926" checksum="D947E984CCABE79E" modified="2022-10-12" version="1">MVIHLNLVIKQNLEPKRMENHQLQEDKASATAKETIDTKDPEQSAGANQHAPNDKVRKIEKVPDFLTDQAKQVFEKLGEFVYDQDQEEYKDLPHLGPYEFENGSVYIGQWKNGQRHGRGKQIRQNGSLYEGYWYQNVACGKGRLIHFDGDIYEGEWRNDNAHGQGKYVRMDGAQYIGQWEDDRQNGEGQEIWPDGAIYQGQYKNGIKDGRGTFKWADGSIYVGDFYQNNIQGQGEYSWEDGRKYVGEWKNNKMDGKGVFTWLDGRRYEGQYKDDKKHGYGEFKWPDGRVYKGEWSNGKQHGKGIYIGSSKVEKEGEWQDGKIVRWIRRGDQPIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PEL0</accession>
    <name>A0A8S1PEL0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1150092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PEL0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="93"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="220" mass="25973" checksum="2ED1548C6B07D980" modified="2022-10-12" version="1">MKNCSQSFLFTTKLSQIIRNGCTVTLTVYHNRLLLSGLRKTSRSEKIDKEINFQTTHYVLNWEYSHHSKDIEGFTLYKQNTKRYTFGGSNDQLSQLKNILSGLIGFTNWEQDYKFVKHVRNSQKCQIMLVKQENQFQVIKLIDYQETPEELMALKLIQKNPHPNVLGFNSYYIDNIHCYLVMDYLSQGTLYDLQKISDVTDSRRFKSFALIKNHTSRYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGM0</accession>
    <name>A0A8S1PGM0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1170078</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGM0"/>
    <dbReference type="OMA" id="HMPQLCA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004531">
        <property type="term" value="F:deoxyribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09120">
        <property type="entry name" value="PLDc_DNaseII_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09121">
        <property type="entry name" value="PLDc_DNaseII_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858:SF23">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03265">
        <property type="entry name" value="DNase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035748245" evidence="3">
        <location>
            <begin position="20"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8102001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="357" mass="39796" checksum="FCDC33732B5EAA4C" modified="2022-10-12" version="1">MASLKSFLLFSSLLLLVFGQTCIDHSGNAIDWWFILKMPTDKTFSVRGMDYLYCDAKNNCGTFDWQTDQLDDLTSPLQRTIAQIDFHDDNVMSVLWSDQPWNKNTISDRAHSKGILSANINGDAFLISHSTPTFPMLDDAYDQIVLGMPSSSQVYGQHYMCLSITTTEANRLATEYIIAETLTNRANSPAAFATAFPQLYQLKTNSRTKTYKTESGTVLSAALQDSIKISSKGGFTLTAYSKNENLVEDFYADVVAPALGIDFIMETWGNGTGGLQDPVCDQVPKSYSNLVRQHGAFTFSYTKDHSKFGITAASNNVCFCDLNRQTTQQKRGGVIYCFQHDSLWSIINKAFISRQTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGU0</accession>
    <name>A0A8S1PGU0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1160073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGU0"/>
    <dbReference type="OMA" id="YEICLAF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="20419" checksum="09CECA70C88E1A2D" modified="2022-10-12" version="1">MNFNSNHQKFSKNQIHHFSRLVQILEKKAVMINFDYIRTYSMIKKRISSIDSSDIQQLNQLQQNQHSQQSFRRSSQLMTPKGSMLQAIQELKIKNITEQINQQQYETSNTKQGFRKIIELERRARNIKANLKDNKILLKSNEEKFYEICLAVVLIFFIFTFTYTIIHELKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PH22</accession>
    <name>A0A8S1PH22_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1180191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PH22"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035746727" description="NADH dehydrogenase subunit 6" evidence="2">
        <location>
            <begin position="21"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8102600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="14067" checksum="663D2BBB143013CD" modified="2022-10-12" version="1">MKKLLAILILLFSIVGLISSFLNVKQEILYFEVGFISYSIFLVIICLKGSSEKVKAIFQSTNILLCILQIHTNYYSTLQCINLSGQNIMIFNMIMFYFSSIKEAPIQLIFFFVSRCVITGINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIB4</accession>
    <name>A0A8S1PIB4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1180082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIB4"/>
    <dbReference type="OMA" id="MGICAFD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="35235" checksum="4B27042885E0089D" modified="2022-10-12" version="1">MGICAFDHKSVENQELQQQAVDLQLDSNGKVLQTQKQNTLDENQAALKIQNSFRIKQAKQEADEKKNELLLDMPKDWIQYNSKFIIPELPKQFKENVQKSLTKQDRLLNSMKAPDGSIYFGQWNRGKQNGYGQMIKPDGTYFEGIWTQGKFQHGGILFSNGDYFIGTETNGERVYKNGIIYIGEVQFGIPHGRGSLHYPDGSTYEGEHNNGLITGIGKFRYQNGTYYVGQLLNGIMHGKGILIETDGNMYEGDFVNGQKEGKGILKYPDRSQFEGYFKNNQRHGKGKLIQRDGQVKDCEFQFGEQIYPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PN55</accession>
    <name>A0A8S1PN55_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Arginyl-tRNA--protein transferase 1</fullName>
            <shortName evidence="5">Arginyltransferase 1</shortName>
            <shortName evidence="5">R-transferase 1</shortName>
            <ecNumber evidence="5">2.3.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Arginine-tRNA--protein transferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1240090</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an N-terminal L-alpha-aminoacyl-[protein] + L-arginyl-tRNA(Arg) = an N-terminal L-arginyl-L-aminoacyl-[protein] + tRNA(Arg) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="Rhea" id="RHEA-COMP:10636"/>
            <dbReference type="Rhea" id="RHEA-COMP:10638"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:83562"/>
            <dbReference type="EC" id="2.3.2.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the R-transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.8" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PN55"/>
    <dbReference type="OMA" id="KETHDMA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004057">
        <property type="term" value="F:arginyl-tRNA--protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017137">
        <property type="entry name" value="Arg-tRNA-P_Trfase_1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030700">
        <property type="entry name" value="N-end_Aminoacyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007472">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007471">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367">
        <property type="entry name" value="ARGININE-TRNA-PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367:SF1">
        <property type="entry name" value="ARGINYL-TRNA--PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04377">
        <property type="entry name" value="ATE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04376">
        <property type="entry name" value="ATE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037207">
        <property type="entry name" value="ATE1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="N-end aminoacyl transferase N-terminal" evidence="6">
        <location>
            <begin position="17"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="N-end rule aminoacyl transferase C-terminal" evidence="7">
        <location>
            <begin position="348"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8104605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="588" mass="69876" checksum="3A5F3CDB4338AC99" modified="2022-10-12" version="1">MNIDNINLAYFKGDHSSTCSYCNKGKSYSLGFSTNKLYPSTYQSMMDRGWRRCGTYYYKYNFTLNCCLAYTIRANALLIQHKKGQRKIAKTFLNVVQSDILKNKVEDMKQTLELINNNIKEDKYKKTSNKQQQKQEIQPKQKLVQEKQQQQIVSQEIINSLKLHFQQFQQIIAQNIQEVVQLFKEYFEQKELIIEQVQQIFQINLNIDTVKIFPSQVNHKGDYYTNFIMLLYGLNKKQLFQDFPVNKFALKFIPFVQKYFISNQFLFIQETTGFLSIFTKDNLQKANVHLIQIQKSIDQEKNKMLIEQNDVNQQKQNIKLIDTFTFNSNSFQIGNMKVLLKPAKCSNENLQLYKRYCKEIHNNIKENKTKYKQFLCEEGLDSQKLIQSQIDASKILFLGCFHMRYYLGDNLVAVGVVDITETSLSSVYYFYDPIFEEFNFGTFGALVEIEYVKLMNQYFPDFKYYYLGFYLQNTNKMLYKGDFEPCELLCPETYTWVELTKELRSEIDKRQSQDINQRQIRISQQNIEIIDDMKSNDKSFYGQIQICLNDQIMKLSESNLQVEKLTKYFDNAYCRFGRQLMEKLIFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUM8</accession>
    <name>A0A8S1PUM8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1320073</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8106955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PUM8"/>
    <dbReference type="OMA" id="VFEIPIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090734">
        <property type="term" value="C:site of DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052639">
        <property type="entry name" value="TRAIP_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46569:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RFWD3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46569">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRAIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="17"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="95"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="234"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8106955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="336" mass="39940" checksum="C0689FF6C026C280" modified="2022-10-12" version="1">MSALKAKGRKVFEIPICTICLDPMIKDLCVFTVCGHVFHYNCGVNCHQNSRKCPNCRMRSTELQSLHYAVEEITGIDEQMSQLLNDLTVDQKQHMAKLLADHEELLHENQRIKQRNKFVEEKNESIQTEVQHIQAELKRSLEKNKELEIKEKQQQSKLKTLEVCFQQLEEDNKKLKQEVEKNMSLLKGFDYLESLAKVINNPNEDMYLKLIRQKSPIEQAKQIYDLYVIKVGQFKNVQKEVTQLQEQREQQQKKYEQLLQKYERMKDLYDEIQKENLSIKELSRNQNQNPIQKIIEHYEDKQENEFKRKPIIMQKQLSFGCSQLRKQPLSQSLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PWY5</accession>
    <name>A0A8S1PWY5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1340135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PWY5"/>
    <dbReference type="OMA" id="NSTPWNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="68" mass="7802" checksum="A77D7027E204D7BE" modified="2022-10-12" version="1">MLNHGLNSTLQNQKTVKKDLLDNKHGMLFNPKSMKLKLLKAQQDIVDRLQEHISEKLSTTAQFIASRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZF4</accession>
    <name>A0A8S1PZF4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1370142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZF4"/>
    <dbReference type="OMA" id="DTYHYAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="136" mass="16657" checksum="BFD5166FCD631E1A" modified="2022-10-12" version="1">MDYIYIKQHLDNDPKMQRFIRNRILYQTSDTYHYANYKKRMNQSFDQSIHQTTLQKYNDLSYLVIDKLRQSPTRKSYYSRPTSATQRSPYIFQTLQTQESQAVRNFKRKLNSSKRCAQKQKKQPIEYTCRQYKFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q074</accession>
    <name>A0A8S1Q074_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1390079</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8109087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q074"/>
    <dbReference type="OMA" id="MENEYHA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000919">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="11">
        <location>
            <begin position="282"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="297"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="302"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="315"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8109087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="351" mass="40112" checksum="1FB768D4FD206D7D" modified="2022-10-12" version="1">MGNCNITDKKDFSDSSILSVHNFEFIEVLGKGGFGKVWKVKKRKNKSYFALKMMSKAKIIAKKSVTSVMNERTLLCQLRHPSLINMVAAFQDRENLYLVMDLLSGGDLRYHIGKNRKFSEEETKFFCACIIIALEYLHSQGIIHRDLKPENLVFDSEGYLRLTDLGIARIWRPDNSADTSGTPGYMAPEVLCRQNHGIAVDYFALGVIVYECMLGRRPYVGRSRQEIRDNVLAKQVQIKKNEVPTTWSLEAADFANQLIQRKPAQRLGSDNPEAVKNHSWFNGFQWNKLLNKELQPPYLPRNYSNSGGSTQDTEQDTKENDLMLRRNSIQAQFEGYSHDSNEPITAEPSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q0P0</accession>
    <name>A0A8S1Q0P0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1390109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8109124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q0P0"/>
    <dbReference type="OMA" id="QTFITFD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="65" mass="7460" checksum="BF4F4F68D3920CBE" modified="2022-10-12" version="1">MDGYSLAHDRNVVTIFSAPNYCYRCGNQAGIMEVDENLRQTFITFDPAPRSENESVPKRVPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q198</accession>
    <name>A0A8S1Q198_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1370144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q198"/>
    <dbReference type="OMA" id="REPRMTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007062">
        <property type="entry name" value="PPI-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398:SF20">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY INHIBITOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398">
        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04979">
        <property type="entry name" value="IPP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8108608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="155" mass="19107" checksum="7D9D48618352E8E4" modified="2022-10-12" version="1">MKQSQKGILKKDTQHDIDMKKELHWDEYTIQQQDLERGNKTKIDEPKTPYEDEIIQQNQQEDKIEVEDEIEQDLIQAQLNKQKLQQQHQQKLDIEELNRRLKEDLERERKNMDDDSAEEEEKRKKHEEFVKKRKQHYNEKILIKEAMHKKQFEDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1E4</accession>
    <name>A0A8S1Q1E4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1410013</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BRX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1E4"/>
    <dbReference type="OMA" id="QMFIKMF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10480:FF:000009">
        <property type="entry name" value="Ribosome biogenesis protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026532">
        <property type="entry name" value="BRX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRX1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Brix" evidence="6">
        <location>
            <begin position="12"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8109509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="249" mass="29359" checksum="3587DEAB18D3F95E" modified="2022-10-12" version="1">MAEKQHKVFYRNHILIVPTRNLDLQHRHFMLDIMSILPHSKKTNKIKYEQLRQVIPSLCENHKCNSFIVFHTISNQLILVFGSYPSGPTVQFSVLNITTIKDLQLAGNFSKKGRVLLQFDQRFETIVKYKLIKEIITLLFNVAQARFTDNFIDRIFTFTTEGEAQNRIWFRQYEIYQQKELREIGPRFALELISIDEGLSSGNILYQGSQINASQTQFKQKIRKSEKKFEKILNEINLKEEEIEVEEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4W1</accession>
    <name>A0A8S1Q4W1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1410119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q4W1"/>
    <dbReference type="OMA" id="PRQQEDV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="226" mass="26917" checksum="54DF666C19EDE92C" modified="2022-10-12" version="1">MRIFRQAFEQLGGSRSRTKSDPRQQEDMCVVPPEEYFKLDRPQPLTRQFFTMLSTLEQKIVKECTVERVNQVVQMYQQCIEFYDSRKDDIKYYFVDKIQNALYEMQQISCLKTRLVTEVQSPVNQKINNLSPILIEEDEHYIRNKKKEKSIKLAIQEQVIQVVKQQPEKVQGMVDEYHEKLDQHASLIKKSVNQQEHQFNERLRKRSINKKSLSRCNSSGILEQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q785</accession>
    <name>A0A8S1Q785_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1470086</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q785"/>
    <dbReference type="OMA" id="MAPEHRT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000082">
        <property type="entry name" value="Shaggy-related protein kinase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF0">
        <property type="entry name" value="PROTEIN KINASE SHAGGY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="28"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8111379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="359" mass="41486" checksum="E76636A3BC45B27A" modified="2022-10-12" version="1">MKQRLVTQADDTPQDNQIQNGNKLSNQYQICSVVGNGTFGMVYLAIDTKTNEKVAIKKVYQDRRYKNREHLIISELNHPCIVKLRQAFFTQGDNAKNTEDIYLNLVMDYIPETLSKVIRNFKNNKKPFPNQSLKIYSYQMLRALAYLQGIGICHRDIKPQNILVNPINHVLKICDFGSAKRLVAGEPNVAYICSRYYRAPELIFGATEYTTAIDMWSIGCVIAEMLLGEPLFPGESATDQLVEIIKILGTPTIDQIKQMNPQQQQFKFPQIKCHPWAKVFGKFKTEPHFIDFVSKILVYSPRDRLKPLEALLHPYFDEIRQPNFGDGHLNLPNFFDFYKEELQIQPEIAHKLTPSWIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7D5</accession>
    <name>A0A8S1Q7D5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1480027</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7D5"/>
    <dbReference type="OMA" id="PRRYWIL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049680">
        <property type="entry name" value="FLVCR1-2_SLC49-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 49 MEMBER A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8111536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="442" mass="49735" checksum="3AE235DF813DAB66" modified="2022-10-12" version="1">MHPILSFVAAQTISNYGWASATNLGVILTILGCGIKLLINQAGFIVLIIGQVLCGIGRPFILNSQASMAMSWFHPKTRIMIITLLNVVNTLSLVVSAQLPGIMFKSYNLEDDTDYENGRSRMQRLCLLEFYISLAIIPCLFMLRSRPKQVPVKINKIERKSGMLKIMLRLFKNRNFVLLFIPFSLYFGILKAYLVIMELLMSPYKYETDQVAGVVSFPLIGGLIAQGAFPYFVNKYQIQKAFVRFFLLFSTLAMASLYFALKSNNVIYIYIVITLLAICVMPILPVIMDMGCDLISPIEPSFAVGAFYMGSMLFFVAFTYILTFITGKNKDDKRVLYTNIFSTIMMIIGFLCSLFLKIDNEYYALDIEQDKGSLVHPLEKGDSIVKEKCPSLVYRGTQSKMSISNQKGSMFSCSHNNINIVGDVPLIPGEEADEPIDQDQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8A4</accession>
    <name>A0A8S1Q8A4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1490049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8A4"/>
    <dbReference type="OMA" id="GQKNESI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="109"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8111809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="284" mass="33658" checksum="1C492820C4312079" modified="2022-10-12" version="1">MDDKLYELFQKTLIENSNVNIQKIVQGQARVIQQNTQPLGQKNETISIKDVQKKLEAIEKNLMIQDSARKRRTSRYHSKKCSVKMSTQNGSNSPSPYQLDFEDAKDYEVDILQKENQRLQERLEQLKQQNEEDNSEDLNREKEILQKMKKDYQIKDEELKILNNKLDLLEIDLNKKHSQLLQKSKKLEIQQQTIQKNEEQIKEKRDCIDALQQQINLNISQVISLNKNELKQKLPQKLGVQLDVIEQLILSTFKERQVAQQEYLCLRQLKNVIKDQQKEFLKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8Y1</accession>
    <name>A0A8S1Q8Y1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1500053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8Y1"/>
    <dbReference type="OMA" id="IFLWHYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8112136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="381" mass="45001" checksum="92AA86F66B41726E" modified="2022-10-12" version="1">MSLFSKFTSIFGSNQTTQASSTPINQQLQEILTQIITSNGKLDMKQKGEVLAQYLLDRDEHIEFLFQFLETKSKDLQGVQVVNLLAAIHQQFQYNELIQDVAIKLRETKMSWVQLEKQEWYSTQTEPDQQKYQQNQKILTEYEDKAQPARIYAQLCYVYLQKLAANVDLYRAVLKFTYPYVTDKEYIEAKLIFLWHYKIQNLINSGSILIQFNPNLIEIQLALYLDVWRFQKFICTEIEKIIDQYATLPNSDTLSLYEIYCESIKHYEHLNQFHQFTQTITKPPPQCQINNTLLQEFFAFVTKLKVLNQFNFKKSIKVPNKQDPIMGIPTKNPNVINIRRSSQQLDDSQSNESEQEDDFTNNKKFRSDHNRVQSTFQYVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9A5</accession>
    <name>A0A8S1Q9A5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1480026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9A5"/>
    <dbReference type="OMA" id="AKQCCNL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="221" mass="25971" checksum="2DBD87274B2366C8" modified="2022-10-12" version="1">MLDQCQYFTSDYSYQFLCHRIEKRIYPAHYKVKNRLINSFLGKIQIKPMPFEIVSITNMTNVSKHIAESEIIYENAKTIDDQEKTQVPPNELWEKAKQCCNLLENSKVEAQDLIRVSHLYDQILDILQPPKPRNVYFISPHNSDTEIKQKKLVYNPEALNNLIKRLRSGRVQSQTRSLFRSTQRMQTSSIGKQIQKTELNEQLSAQALNFLKQLKKKQLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QD62</accession>
    <name>A0A8S1QD62_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1510041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000156">
        <property type="protein sequence ID" value="CAD8112370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QD62"/>
    <dbReference type="OMA" id="WQFKKQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="114"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8112370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="188" mass="22230" checksum="FB0EFF38E9F877A2" modified="2022-10-12" version="1">MDFEHSDQFTQRVEQIIRNFLHIPEEGNPNWKEINQKLDQFQSQLVDIQLSILQYLKSSFEAIREQLQCDLGGTQIQTLSMSSSLNQQNHPLKPDEVKVNERINQIGLNVYEIEKNLEIKIDLLNNKVENIRINRDELQEQIKSFDARMESQWQGLANVSDQIWQFKKQFEPLLIGVQQQKKKFSLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDL9</accession>
    <name>A0A8S1QDL9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1560103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDL9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="11457" checksum="2398B946DC2D1856" modified="2022-10-12" version="1">MFCSTYQHQQLRQYMPAINFLIQNFLIQKCNSLVFTKCTLETYLTYGLCKNYCPNYYQNNLNNEFFQLSPKNLFLQCSLLFTISIQYLLIKNDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QGF5</accession>
    <name>A0A8S1QGF5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1560037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QGF5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="134" mass="16153" checksum="91320D7E04E75077" modified="2022-10-12" version="1">MVPNGLSILVFGNHQQNYRKLCEFYNSHKRYVMVQNLLIDQTIKYRQYLIINSSIWIFKNNYLKYYINLSQNICAISYYLSIWHINYIEFNYAQIHNALFVWTHTAISDLRISITSIHSYLLWTLRVDKSEDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QGJ9</accession>
    <name>A0A8S1QGJ9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1590104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000164">
        <property type="protein sequence ID" value="CAD8114416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QGJ9"/>
    <dbReference type="OMA" id="KPEDSHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="378" mass="43149" checksum="9E036501CEC6DA32" modified="2022-10-12" version="1">MSTQQELPIFNQQTPQPSSQISKVQYPILSNVVPAAQYIQLDDVQQAVAEKSVVIEINSDRSAFLIEYYKKILLQHSIILGLFILGLTKWFSSIVVIHHYWWSYFRWTWYVVLGLLIAIALGLPILRKYRCIVSQQSKLFHFQTVLIAFLLIGIAGNQRGNSNHKTVNIWIILVLVILIGHDLLQIIIVKTQRTHYYTALMKFAIMGSLALNFIVGLSNKNLDRAFPISEIIIIYALYYFNQLNDQLLKQTHELPEELSIVERIQQLTLIRNLFNQEARQNQIIEQIQDTHCVISKHLVIVIGSSVGYIAVLVLSIVTGNGFLIFTLILGSISMLSVILETQVASRSLKPEDSHYAVCLTYLDMASPLRLLLRSLFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QMY1</accession>
    <name>A0A8S1QMY1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1880012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000197">
        <property type="protein sequence ID" value="CAD8117103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="604" mass="71513" checksum="60FE0D93E7C56474" modified="2022-10-12" version="1">MDIKLKVQILDLIVKPVNFIIEDSNFKIIQFFNCIHDQRYYISLNGEDCFENPIKNCLYAFEVSRYQKKINSLDYQFTPYFDNTYVICGQCQQQYAFNEETNLCQLQKSSDCYYSYLSAITQFEEVCLFGPKIKSYRDPVSFITESSCLNYNCHICIIGQINIKVSYLCLECNNGYYAEILTGLCVPCPQTLKCQVCYQQNKISKDKWKNQVRAYYRQVIDDDLQSHSFIDYGLSQNPNDYEIICQFCLQGYEIHNDKCIQVCSDTCLKCKIINDENICIECPSIQGKRSLSLHNNQCIQCSRNCVFCRQREEDEIKLINPIFNNQEFNYYSNQCLQRQKGYFDKDLKMYVDCPFWDCMKEIQINLYLHCSQEQYYQQIQSLEREEDVRSYKQSNLLLDDLFSTSSFQEFETPEFYQMANEQVIKSIIIRIFSFESQTCIIYNNKSIKQKFSQNIFSAINIQLELYGNGNTVFLYDKIISIVNFKKVHIEGIILSPLSVHNLKQLFFHSVFEQTVQLMDIRYESNLIQDQSQILIWNSTNIHIDTFFTQNLNLTDIFAFIIIEQIKMVQTIHISNIKIIDCYFKSLSVXSHFCLFRIQSIWYIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QR26</accession>
    <name>A0A8S1QR26_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2290003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000238">
        <property type="protein sequence ID" value="CAD8118209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QR26"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="33" mass="3771" checksum="91E1823C43B33902" modified="2022-10-12" version="1">MPKLETLLQFLLASPWSTSQQVNILGTVIWLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QUK4</accession>
    <name>A0A8S1QUK4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2430003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000252">
        <property type="protein sequence ID" value="CAD8118494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QUK4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="126" mass="14567" checksum="AC7BF5714FAEF983" modified="2022-10-12" version="1">MQQFVVLNIYLDTAKTDQQKFDTCTNIKDNTGLLCGVTFLKKNVSSTPNEVSTCTYNYTINENTDSLKLSQYSKFVTNIFQELHAYSKLVKRSVLYRIVVQSQLPKFIQKPIMFKTIFSSEENVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNM0</accession>
    <name>A0A8S1JNM0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0060085</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNM0"/>
    <dbReference type="OMA" id="FRHEMPR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048995">
        <property type="entry name" value="STL11/RBM22-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF6">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21369">
        <property type="entry name" value="STL11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="202"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8044003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="344" mass="39534" checksum="BD9DD5E0DD15354C" modified="2022-10-12" version="1">MAERGHALKQDISKLAWEETDFPIVCNNCLGENPYIRMLKDRFGKECRICARPFTTFKWKPGNNSRQKQTEVCQTCGKIKNICQACFKDLEFNMGLCTRDKFLGDSKIEIPEHTANRDYWAEQANRQIERLILPYDKPLPMLDQILKEPRLADVNNQLNDGKSEPANQIYAEPLENPQDIDLLYKEKFEAKGLLPPDDPKISSLYISHMSTDIKESDLKHLFSKFGKLNSIKIMEHGQSCFINFAKRKDAETAVNALYNNLIIKDVICKIQWARAPNKKVPIKDLIQSLQPEENNKAQPPQAPPQNIDKRQQSEDNLLLGLLNTQMTYPNQNPYQKGGVNNKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNV9</accession>
    <name>A0A8S1JNV9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0050188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNV9"/>
    <dbReference type="OMA" id="PRHAYVQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210:SF1">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="195"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="422"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="528" mass="62435" checksum="DE8C1299BEC6CEEB" modified="2022-10-12" version="1">MIAFIESQQIEVVQELFRFCSCLQKLHRSLIIIMNNDNNNNPNNNNNNNNNQNNFLSPFPHPLLDLEQFLEFGSSFQFNQFLNFYPIGEQQQRQFFRMNAFTSRSSNQDEEFMLYIQNQSRREPSSPRQKNSNLDKYMQKRAIPIHNEELNKNEPYIKVTVLRNINYPNTIHSNEYFINQQGLVGSHKNADSEDILIGRSHRTEIIPNDIMLPEDRIISRIHCKLVCKNYFRKEQKIKLIYILAIQNIRTSSNFPLPKRAMQIISQFLEYPRHAYVQDLGSLCGTHFKIQKNEPNIMKYDQKYSIGSDTHFNVLFCESYKEKGIEIDDQFYRLLRKISRLKQLKHEIHFSGQSFLERFQKIQTQEDDIEELKIKTEDYKEIYNKLKEYKISIISIKFNGSGIDTGKNYNLFIHPENQDSIEFQIGRGQENHVKINSNTISRKQSRFKYSKSLKSWIIFDGSKERDSANGTWVSLSTIEQAEKKQESDLIVLKNNAEIKISEFILKIEMFQGKKQNKINKQLLNELKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPX1</accession>
    <name>A0A8S1JPX1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0050222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPX1"/>
    <dbReference type="OMA" id="INKWILP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="1">
        <location>
            <begin position="226"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="VPS9" evidence="2">
        <location>
            <begin position="878"/>
            <end position="1011"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1011" mass="118795" checksum="DA9EF15C933D8310" modified="2022-10-12" version="1">MFEMHEEQNNENIAQIQDSNQLRLSKRQSFKSDTILQWNSLQENLQLQYNLLNKENETKNERISILLNTTEKYYQTQLRYKDLRRMYNQQQANNYHLKQCLLGYKFENYHTEIQKFLFLLMENQELFAQLIHSSLDVGVHNLDEFIFDILTFLYDPIIGAGLQEGVFKLLSELLKLELTILKNPLELFQDKSNLVCKFLIGFCKGPLCQSYIQQVCGQALNLLMNVKEELELNPNIIYESQLKFKESKESAKLLKLKQETKVRKLMTNLLPILMQGTEENKDINVYENVRRTNIPDDILNKITEQITPTIQSYCQTPLQRIMDPQLLEETVKILYDRQKVTEMIINSICDNILNSLHNLPFIIRALCRLQYQIFKVHYQQYTQEELFNFLIQFFINKWILPEFTAMFINQQQLEPSQYQTSNITAISKILLSVWKGETIKGLNISLTNVAVFNYFKELIDLPQTQLNFYIKEKSEKHFISYLLSISDLQILLNTISKSRIEGSLGGKQLTRYTDLRELADSIMRYNQKMGNDFMSNNVQWKGQKVLASNMHFTVHRLSDLYNVNYIDIAFKFTEKFSAQDKEELLFISKAKQVVAQFLISIESISLLTKLRNNIILSFDNLIYMYKDSYENKGQIIKQYENISPQLYANYLLSIYKMIPNQYTSNNLSLLCQEMIQEFDQKVTYAKNQQKAMLEVFLKIITQLQTERSEIKALNKQLKTIELKKKLNYFLKNVKIPCLLSFRYGQINLIPIQDISKIKSLDPKDNEKQSFLQIANGEDLNSLQLELPFSSIRDFIDILSGLNHLKFALEGDNVYIEIVQKLFQNYMKLVEEKLEQQSFFPQNQNIKKNNQYIDLCYETAEKSVVRRIMMKYCTVNEIKEDKSFIEQCQKKLKHLEEQQEVKICLSELPMLQKMSESFRQIDQVITPIEKLQMISNCLSNLSLLLKISGIDDNPGQDGTLPVFIYLVAQACPKFMKTNLNITQSLFNFDRWKNSTYPFSSTQLQGAYENIMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPX4</accession>
    <name>A0A8S1JPX4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Diacylglycerol kinase</fullName>
            <shortName evidence="5">DAG kinase</shortName>
            <ecNumber evidence="5">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0060263</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPX4"/>
    <dbReference type="OMA" id="LWEVNIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF121">
        <property type="entry name" value="DIACYLGLYCEROL KINASE (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="6">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8044181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="415" mass="46832" checksum="4EF8DCE38313F3CA" modified="2022-10-12" version="1">MEFAFFLFVNPTSGGNRAGVYLQLDAETIKFNLNQKPINVYFTSLHQRDQVEMNLRRIKEFQQRNVQVRVIVCGGDGTVMWVVDEMHKHGVDYSNCPIGIIPFGTGNDFSRVLGWGGDTDGDLGACLRNFKQQISQWLNAKIHDFDLWEINITVDAQTGSFKRIKKQGDLFQKEILQKDKDVLKQLDKRMSNYFSIGVDARIGFGFDKKRTQSACCNKCVYFCEGLKKMCLKNPTTNQVIQGMQILKEQNVTIEQITATVPTDILFKTKGVNTREDQQPLNESKFILSGDPVTLLCLNIPSYSGGAGAIWDNCRGKVAVKEGTQKLQDKFVNQDFGDGKIEFVGFNSMIGMANERFIHGNAVRIAQGEGPFLISFKKNVSPYFQIDGEYFQAVNPTLALLKRSPIRIKVLMKAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JR86</accession>
    <name>A0A8S1JR86_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0090148</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JR86"/>
    <dbReference type="OMA" id="CCHDSSN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="469"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="825"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="630"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="836"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="636"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="876"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8045123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="983" mass="115518" checksum="FD55FC7886D6011E" modified="2022-10-12" version="1">MNLCLQNRKLNFLQQNNKGPLILFQFTQNFYSIPGKMLIYTEMKSIFKIQKKIQTRLTYEILIIFQYIKTMLIWLRKQKRLALEIYEDYNDSIYIDCQNELIISQFWPLFVTIMKIYLFYSIVLIMISNIEEYESFNSQKVTLYTYLLILLPSTMIIYDRWIIEKLSKRQKVVLTQIIFITLETLLQLYLSQLLMEYESLQRLIGILLIFTIMLIVMATMNNFKLRLIILIKFFVYLGYKTEFTIFQYLFTYHIVFLNLLLVYYWDQQIMRLSKIKHFAEQSLRSIPTAVCILNSDCQQVLFSNRYMKKLIDKSHTKTKTFMTKSVKQDYLFETQPSIMSNYDEIFQFFSQMFANVEEVKNQLPPDSSKFNLDNLDDEQINLNQIIQYIIKTDKKTQGKIYTLRCQFIDRPEMINDESQKVVIEVKMKTLLQYEDNKHATLINLYDISTNLKSIYYKNLNQFKSKVIRSISHELRTRLNAIQGMIQMVQNYNQNFDREVQKYLKAAFNNCRIQNFIIGSIIDYNLLRERQLPVKLDKEVIDLFKDEAELKSIKIKYEDKIQTNNFELLDSEKFQSILIHIISNSVRFSKSDGLISIKLLKDLSNNNNNNNNNQTNSYIQIPDNRAQINKHSKLQVPHSQSPQQQRLNGSECCHDSSNNKQRRTYSKYKMASQFSSNGNLSTNLADVYMIEVRDNGLGMTAERLEYIRSLLNGEDFQVERNSNDASSGMMLGLRASNQLIKNVSGKDDNGNYISIDSDIDKGTIIQMHIKIKLISGENFSSELILKESSVENEGIVHSFQNFNLKLSPWSTVAQIHTKNLCQCENTFMIVDDEPYNLIVLESLLKKLQHQVVKAENEKMVEQFQDHKCKGYKGIIMDYQMPIMNGEEATKYITQICKDKNITPIPIFGLTGFSGEDEINRLLESGMREVYVKPITIKTLEDLISNISRSEYRSLDYVSNISQQFQFMDIEYAEEDKLVLEQSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRL3</accession>
    <name>A0A8S1JRL3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0040081</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRL3"/>
    <dbReference type="OMA" id="FCYINNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052842">
        <property type="entry name" value="ER_Co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057305">
        <property type="entry name" value="Thioredox_PDIA6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45184">
        <property type="entry name" value="DNAJ PROTEIN ERDJ3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45184:SF1">
        <property type="entry name" value="DNAJ PROTEIN ERDJ3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24541">
        <property type="entry name" value="Thioredox_PDIA6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035876420" description="Thioredoxin domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="28"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="PDIA6-like C-terminal thioredoxin-like" evidence="3">
        <location>
            <begin position="262"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8042866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="45403" checksum="4365DDA9E609180E" modified="2022-10-12" version="1">MKTSILVVILLSCTFAKQLYDVKKSYVTPVNEINFEKQINKIRQTTKYVTIVHFYKYSDGESRSFAPKYDEFTNEYKGIFRIASCDCDEAEKICQKESVSKFPTFRVYPPYPVPAIDYEGPLEIDGILKLATKYIHNNIIEINESNINTFINENPTVPKVVLFSEKKGFPLVYKGLSVEFEKKLSFGIVRSSEKALLDRYNIKAFPKLLLIKTNEKKPYPYTGEYKFKPMFDFLNVHSEVFVPGGGSSADSAATKEWMMQVVPQLHQRSANDICLKVESVICVILMNNGDKPNNELVDELKKLNQQYERHINRGTVFKFMWLDVKTESKWGQALEFQGEPKIVLLNPSKRKRFAIHDGEMTFEAIQRTLEKLISGDLKFKNLGESLPDFVKIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JU34</accession>
    <name>A0A8S1JU34_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JU34"/>
    <dbReference type="OMA" id="NENCYLE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035919120" evidence="1">
        <location>
            <begin position="18"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="710" mass="82453" checksum="846626BB06B3E9D0" modified="2022-10-12" version="1">MQIKNLLIFAICYTVFSNENLTFNCSNITDITQCQKDSNFCYIVEANVPYLSAENKIEGVILDKVNDKCMNLNFFTVCDQLYTEKSCKLGTCLWDPIEERCYPWISAPCQSYPKEFCQWNPQCELLNHTQILYLNQNYTLMNNVDSYQQDGRKIGTWLYEFGQIINVIKSEVDQEEYIFTKCTQKIRCEFIQITNLNNADYECSISELNCYYDEQEGKCKKISALTQCELIKWESKCNSGTAPCIWINRKCQMYDNTMVSCSQLDQNRCLNNENCYLEESTCKSYLSCDDFKIKSSCDTSNFFCYFDEYEQQCKQLTKHIQCKKIGKQNCEIFKNCVFNEDAQLCEELLEDFYCQQFVQSDCINTNAQSKDCIWNNNLQICNWNYATQNCYGKTADNCDTLDCYYDEVMNQCSIIQRNTNCEFLGQNACNNENIKKQNMCQWSNIRNLCLSIFDFGCEDLNQDFCKLNHKCYIVQNKCNTRKQCKDNTSIIECQQDTQQYCYFDYDQQACRRVTTQTLCNLIFREDSCKQAICSWQKGKCESVENVSCSQLEIKNCKFSSKCLLQNNQCIPLDICETNNGNKSTCNSDPHHCFYNTDTQKCKSIDGNTNCSSIYGADNCIYGPCTLLRLANNKLQCIKYEYADCALLKKEQCNFGQCAWNVTQCVPYPVILEDQPNDNPDVKPDESGSVQVGDYSKYLHILLNLLIIILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVG6</accession>
    <name>A0A8S1JVG6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0110021</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVG6"/>
    <dbReference type="OMA" id="PRHKDVT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000983">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8046642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="197" mass="22176" checksum="C59DCA6EBC5E1808" modified="2022-10-12" version="1">MAEPLTIKLLVVGDGSVGKTCILLSYTTDKFPTDYVPTVFDNYQSKVQIDGHDVNLSLWDTAGQEGYNQVRQLSYDGTDVFLIVYSVTENTTFQNALEKWYPELNKDKFAQVPKLIVGNKIDMRDEGNSRHIKKSAAEQLVKNINAQLFEVSALTQEGLKPLFDTAIKIAYDKKQKEIMDSKKSQQPAQTNSCCQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYD9</accession>
    <name>A0A8S1JYD9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JYD9"/>
    <dbReference type="OMA" id="WHYESED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="216" mass="23699" checksum="EC21BD0966AA5220" modified="2022-10-12" version="1">MYRSLGLLIRSTSIYNQMWIRGPKKAKVEAVATSDDLINIWKDRKDPEIKEIDEYPLWLLELAVPLDSIDVASIQIAANDKAYHPPASHVRSIVRSIKRQRIILMNKYTALNRDGDVIGDDDLNDQEDQLEHIQQEKLAEEAAAEEAARLKELEEDDEDTPKARKPAIRKEEDDDDKPAAGKAGDKDKAASGAKGGAAATGAGGKAGDTAKGGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZL5</accession>
    <name>A0A8S1JZL5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100218</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZL5"/>
    <dbReference type="OMA" id="NVQQICK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="335" mass="39326" checksum="CDAD508B41E1813B" modified="2022-10-12" version="1">MQKRILNNLLQDKIYIKKQQTFRESKQINTFPILIRNKSCECQECGGGIKKQLEEYQLGQVNESKFVPKLIAQHENIKYCDISQSHELKITKNLFYLITPKKIVLQQNTSLTEQPLISPTLEQPKRNIQKLHRLIEKYDSKVESNVQQICKTMPDAISIPIQKVKIQRQKTKQYSFIRLLPLEDEDDNQNINNANSSNSQQKTPLRVQVYKELKGSSILQQLQTPIFRKSKFNKNQIIDFEDGMRKAMEKIKEMDEISNTSKMISTQINDENKTKSRGSLLLKSFLDCTRQIDKDPNNIKQLDVFLRPESRQQKQMLQSKTFSYQKVYLPKLNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZU2</accession>
    <name>A0A8S1JZU2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0120271</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZU2"/>
    <dbReference type="OMA" id="MLMEYNL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06174">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF9">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8047991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="536" mass="61498" checksum="43B6E04021A61EE6" modified="2022-10-12" version="1">MDKRFFSNQTFHFLTIYFINFDHGILPASIKRIQSDLGINEIQLGLLSSSVYAGIIIGSMIGTGMYDKYKTKSILIICSLFYILNSLQDFIYFPIWIDRYGGKQKDQQLIYIQIGVPLGTISGFLISSLSLQILQDWKYAIYFQIVALTLCLLYYGFSDNNIFSIQIQEERDQSQSFSTVLLQNCKTFFSNPLFVLSMLALSQLFFVLTGVQFWITDYLNSVMLMEYNLASQSFIIISITAPLTGVYLGSQILDNHAGNKGFRIMKIAAIELFLTSISLALIPLFDNQGMIVLLVWCLIFFGSSAIPSLMGIMITSLQREEKICGNQLSHLFFDFFGYLPASTVYGYVQQISGGSTSRKGMVMLSCICFLGLIFLLLGIYKKYNQLRLIYIDLNESEFKNKLRSLSHHSGDNMDDYEKKDFSYIRMQTASKKFKQIIFPHYNDQIEGDQLSENESPLIIGMETQRIRNAYRSQCSYLHICYNVSSIIGRRSLENDLFMIKDDQLQIRLKSQNSFQLQVIGANHLICEERVVHQQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2D9</accession>
    <name>A0A8S1K2D9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0130192</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2D9"/>
    <dbReference type="OMA" id="TEQRNHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000051">
        <property type="entry name" value="Protein phosphatase 2C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="3">
        <location>
            <begin position="203"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="PCI" evidence="2">
        <location>
            <begin position="655"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8049027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="857" mass="98819" checksum="86AB0E913074C9AE" modified="2022-10-12" version="1">MLTQSLQNKFDFKTRQTSQIGIYTLLHNQFINLYNSTKLLIIVILDFSSLKPPRHPQGENQIEQHRIQKPLLQKRYKITSNETEQRNHKISLPSLANFRKTEFNSIENIVQPISILNQENGLRIPNRLPKLENRNEISKSKYEMQLNQAPKDEEQQKPKTKQKKTSYTPEQRISESPEKNISQQSKKKKGQKQFKGMKFIHNTKAGCQVNKQIKTNQDAAIIFPQNIQSQNYGLIGICDGHGVNGHLVSDIIRQRLPIYLEFQLQSQNPDMEECFKNAFELTNSEILQSQFDTALSGSTTVLAMIQQNQLWTANVGDSRAILCRNSNGWRSIPLTRDHKPSDESEKQRILQAGGRIHSSRDFYGNDVGPERVWLSYVDAPGLAMTRSMGDKIGAQAGVSSIPEVFEFTLSQNDKFLVIASDGVWEYLSNEDVMNIIVPYFEEGELEQAGEKLMMEAINSWKMNSPGRDDITFIIQLCVVKLIYQLFIDMSLHFQYLGQGLSSQKTMELLQKHCSSNDHNKIADFGAVFDELLEQQKYKELIVSTLDIINLSQDKTLLQPIYSAIQYWMTKLESKEQQIIIDLFLKHIEQINVEAIFKTKIVAQLFNALSSSSLSQNLFLKLIECAKKWNTQQLIISPIISNIKEFLNLWTLSDPEILKILNAISELIDPQDQQYVIQISEYILRNINTSQEQYIKTFINFQNINQSFLKLCEITQCSNYQIIEKTQVAQLIKLVLNGDLSGVQAFLVKEEVYFKQINLNVEQYLNQTRIAKFIQLSSQKQSYSYQEIAEALNIKLEEVEIWVIHAIQSQNVSAQIDQSQQRIFILDNFKKLLTKDDWQNLHKKLSALLTKLKNVQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K379</accession>
    <name>A0A8S1K379_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120348</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K379"/>
    <dbReference type="OMA" id="NDNLWES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="872" mass="105118" checksum="8BB10E8236A41C6E" modified="2022-10-12" version="1">MNIQLAQKVVNQWRMFVEKKREVRSILVTQYKQTYLKVLYSYKEFKKKKFQQYIITNASKLIYKALKINLQRVQNLKQFIKHNKIKRFYILKQQDNIFHKITKIQSQNAILKTIRLPKYTKCEHKNLIPLIFRRVQKAVILFNFHYMKKYYGETTIDKDMIKNEFLFYFTNYVTVKRPWKQYQLTTSEYDRYQHVLQKVDKFQSEEYTLLKSVELKNINILKKIYLYNRKNKEQQLQIYFDYQLNEIGAACKIQKFFRGKKDRKIKGMQYSIIIGLINQSRAIFVIQKWFRRIRQFHRNNFFKDISFFISQIPTEHLYLDMEIYQKIEQIVQNQQYTIKFLEQYNTIVSDAESVRLMFSSQTQCLNQSTYQQTISQLNNLPIKVIPQWLIKQIQMVPVQQQKFIDYTIVKQEISNSKWSCRNRQYQKKQFEKLNELNFFKQCDIYSLLHLGAKISFDRICKRSYIRFTYKSTSEARYRALALALITYKFKFNGVCIQMLNDNLWESPYQKQEQFLQKYYQKIQKAYKIESQELLSSKLQNSTSICLILCQNEFQAEQFKLKQNEIQLQFFNDTSRIPLNFIWMSTQQIIKKVPIKIQNQQIVQTNTMKLSSILQNSQILQNRNPNEDDQQFEQSIQSKLFVTNELSPRSPKKDGINKSTQLPLKYSVLCIDNPDIQFQEPKFKRRTEQSYLEFAMTSRFRTQNNPLNSSKVNGLYDFESNNAQNHKKKNNQDLENISENTEYTILQRRYKEFILNDNKTKGESLKSQKPNEKILLSEFTNLINKQGKKIINNQIQTQRAKSIDRQTKKIFIYSQLSQSVTKREDYKDYSLAFPKIEQKSSKKHTLDSRLSNYQKYQVFPEDQYTMAKDNIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3M6</accession>
    <name>A0A8S1K3M6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0110445</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3M6"/>
    <dbReference type="OMA" id="RSERQIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31652">
        <property type="entry name" value="LIMR FAMILY PROTEIN DDB_G0283707-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31652:SF0">
        <property type="entry name" value="LIMR FAMILY PROTEIN DDB_G0283707-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8047470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="756" mass="90154" checksum="FBC4E7F5795F4EAC" modified="2022-10-12" version="1">MQHREEMIKQLQNIQDPYVLQKQRQIDVKRKYEQLLAQVNLIYDEDAKERQYQVDFQRFIHFYKFLARRPDSEQSNQSIVNRLQLWVPDTIVLNDREMPNYWLYSDSRGYVFRTDAFTSKNVVSKLANYTSPDELVAVIKKQQFRNMELVGNEIKLVCCRDLQSQINVNFQNRMDLTIIQKFIKSNGPKAFICRTIWRKDKNPFCYIVTNKEDYFSQTTNPKSEFTKYITNVNQQNSCTIVNTCRGKYVDETVPYVKNIINYIYIHLHISFKEFAADFIKDESGIWWFINVKGFIIDKSPDKILWKSITHYGEDIHEEGQPIIQQQQSKIISPKNKTKVADMCQKLKICKYCEQSYPEQYLQYKMTLKMIIQTDKHLYWRGKTFNWIERSDVTNLEVSNLYHEHKVCKQCYTLYKETEQLIELQLQFNKQLGLPSDYDTINQMLTLKANQVNNESIKTGIDESKQIFNISATKQLILNNQSIPIIHNTVIKTLNRFRFMILIHSIRDVPQNVDLSKHYYIECNIFDQKFRIKLDLITGQIFDKGYFLTLNRMRLYYFFSDQRKGWVEYVNQIKILPMYLYQDNIKIGTLEIELQDLLSERVIRREFLKVFSVKDCYPILSWSLNLTLGLVDSGPANVTRIKLQEHFGIQLPNPDYCTCEPLPAEWMTILNQKKDVDLHRSERQITNHTLKRVSTAHAKLQKSRFEGSSLHQDMESFNDSTKELYSQLNLQKEIKLYEIDEDERKNKKRRLFQQYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8P2</accession>
    <name>A0A8S1K8P2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0110127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8P2"/>
    <dbReference type="OMA" id="KEFSACK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0072">Autophagy</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8046838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="150" mass="18183" checksum="334A0CECF0825F61" modified="2022-10-12" version="1">MDKVNAEEEGLINRKQNKEFSACKNYIPVIVEPYPQTKLKKIDDNKWLFNKDSTLYPVMYKIHETLNGAKNMYFYVKRQNGRYALLKHENKLQLVFKKYKSNDGFLHVYYHNDMINRSKLLDYCFYFAQIVIAFYLVLFIYGYLKMHEYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAJ9</accession>
    <name>A0A8S1KAJ9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ceramidase</fullName>
            <ecNumber evidence="1">3.5.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0190048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAJ9"/>
    <dbReference type="OMA" id="RTHYPEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017040">
        <property type="term" value="F:N-acylsphingosine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28583">
        <property type="entry name" value="ACID AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28583:SF1">
        <property type="entry name" value="ACID CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035841398" description="ceramidase" evidence="2">
        <location>
            <begin position="16"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8052169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="366" mass="42560" checksum="FF5E82BD022346F5" modified="2022-10-12" version="1">MQYGILFMFLSTVTSFNYLPEIEIDLSATPKQRWKLAIRTILDLYGYQNSFGPVFEYHNKNTFYILEPQDYKTIANAIRTHYPEYSQELDGIVEEFNRSEITFEYLSVWAYFHEIAHISAEIIESTAVLLSTADQIIHGRNMDQGPPQIRNITLHLIIKKDGKYLGESVDWQWFKVGFVTLLKYNVASLDQNWKFSDPLTKDQLFTFIEQGVPSLAWEYRYVLINDNLNTFDRVVLYLEQNQVACAFYNVIGGTSKNQGVIISRDPFDMNPTISLNTSILGQTNYQYLVQTNYDHWLPDPQNDLRRTIAENLLGQLQTNLRNEFGVFTVMDTYPIHNEGTFYTIIMNAKYNRLIAFGQPSITQTDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KBQ0</accession>
    <name>A0A8S1KBQ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0140436</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KBQ0"/>
    <dbReference type="OMA" id="INYMAKG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="47"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8050326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="349" mass="40856" checksum="8630B099C25C9DB0" modified="2022-10-12" version="1">MMEKQNQEIEIFDIEKQIIQMNQYEKSVLSQHQIQNFIRSVIRIKDIQVLSNLGKGSFGQVFKVIYKNKIYALKQQLKQQVLQDVKYIQSELSIMKYLNHPFVVKLHHSFQTQHYLYMLLEYCDNGDLTNYMTKGTIIQETQAIFIIAQIILAIEYLHSLNLIYRDLKPENVMISKNNYIKLIDFGLSKEGFNVTHTFCGSPAYLSPELLNGHGVTQSTDIYTVGLLLYEMLSGYPPHFSNNIRILLNGIQNEELRIPKHFSGSVKNFLEQIITKNPNDRLSIKQIKNHIIFAEIDWLKLQQQEYIAPKLKKKETNKLTQTTKLIDHDYPQDGERINEVFGWDYSCQID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KBT7</accession>
    <name>A0A8S1KBT7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190283</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KBT7"/>
    <dbReference type="OMA" id="RNKLQPI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="221" mass="25957" checksum="A3E3383C075E73DE" modified="2022-10-12" version="1">MNQCLNKLLVLFNLAYEFILQKLIEILQIGGIIRPAIREPPILTLELQPIDQSLKQPLTLHSSSSTPVFTKNESIYRRAESEKEFRKCIIPNLGDTINKVECYSRDISLLNLFDEKQDSNQNQNQLDNHNIRNKLQPIIEEQKLCYNLENIEVQGINQYSINHQQNIQNQQNNQHSEEQITQEQFYSIIDNPNKITQSEQFYSIIDRSSFRHSFNKNLPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KC31</accession>
    <name>A0A8S1KC31_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0190348</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KC31"/>
    <dbReference type="OMA" id="GRTYNDR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000129">
        <property type="entry name" value="Dual specificity protein phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF511">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="18"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="79"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8052739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="326" mass="37276" checksum="583B78FAB340AEF4" modified="2022-10-12" version="1">MLQPRPPFPSGQMDLILGKTENYGALYLSGIEAAMNPLLLQQNQIGAVLTVGTELANLKFDCEQKLIMLHDTAYDPIRRHFEEAIHFIDEQRKTKNVLVHCFVGVSRSATLVIAYLMQTYNYSLQVALTFLISRRPQINPNPGFMQQLQQFDFELNKRRQQRPASTQYIRCYNERDRRESMEVRSTRQIPNQSPLKRVNQKAMSAKSLFLTPKKSEAQNYFISTPAVKPNNSIEIKQIIKSAQNLDSAVLRQSNYYGNNTKKLAETAINGFKSASSFCKTFSNHFPMAKNQMLAQTQTLLKRTQGSGLCQKGRQPIRAQTKIEYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KGU9</accession>
    <name>A0A8S1KGU9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0210169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KGU9"/>
    <dbReference type="OMA" id="INVEAHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8053887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="28449" checksum="970C13EBD6ABB95C" modified="2022-10-12" version="1">MLILIVLSFAYAQIQDEQQLTNAACVIFSRYYLQSNQELAGRAVGEMIQLQQLTKEDAVMFAVASLVESCLESISTQETMEIIQQLQSQKINLEAHQHLVEKADLKKYGSKKKSAKKNEILKIIKSIDDMVNQQKREHEGGDDGNDQSETGRKRQRNKGKQQELPDMSIFELKEWLVLSSFVLVGVLLSYIICCVPERKKKEKKDKTKVKQKDQDADKLSPKKEEENSQGEDESSPKEVAENKEKKKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIB3</accession>
    <name>A0A8S1KIB3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0210223</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KIB3"/>
    <dbReference type="OMA" id="KSECNDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="379"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="638"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="215"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8053963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="872" mass="101606" checksum="90EBED407146116E" modified="2022-10-12" version="1">MKKLSQQMKKIAGRNSFQESPQRQEKKNIKSSLGTVKTERPVEKGKAKLLDYFKRERNSLAQAVNTMRSKLKGSKSIDNVFLQTLKSDRVKTSQPNIAELRVHTDEGNNSKRPTLKNKNLSLATVSLEKKEFFKHPLTTKTSYNQTPTGQSKPQLKKQKQKDQYLFDMLKNAQMIQNQYQKYFNQKLNKQSILSDTSTNHRQSIEDILNYQKGHKVSSQIIQQKPIKKTDNESKSMNQGHSKTEQTPQPKQITKTKNDEILDYDPKQKLQIILYYKNSKYYYLFDFANQSTCNLYNYLIQQIASIEKNHLQQNGSTDDDQIQAEIKTICQFCTIQKKVPFDYYLSLPDLSLSVFQGITLSLEPLYSQPQTTKKVGLKDFQLIKCIGVGGFSRVYLVRKKDNGIYYALKLIDKKFIIDNKKEIIVQNERDIMIKMDNQYIIPLHYAFESKFYIAFVLEYCAGGELFYHLRKLKKLNEQDAKYYFVEICIGMAYLHSQNIVYRDIKPENILLDLQGHLMLSDFGLSKPDMAAEDFAYSFCGSPEYMAPEMLMKTGHNYLVDCYCLGALLYELVSGLPPFYSHNTQEIYSSILTENVQFPDYVQISNQLKDLIIALLQKDPTERLGQKEGVIEILTHPWFSDVDFEKVVNCKLPTPYKPEPLKYNFDEEEFNKGDAEFRKQFAINMLNEFQNVDTAKYQLSNFYYQKVKQDEYINRTNIVNINKLNSEFLQKEPLSPSRQILNKTKHSPYESKKSLKSNTKSECNDYFKKHNLLTKSSQIMQESLKFGHGYSKTQQQSQISNQELKKLKLMFDQSKQLLSSDRLTTIPDQNQKNQIERIKTEQFGNLPSPKTTTHSTLNKLTKYQKLFGSEKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKV5</accession>
    <name>A0A8S1KKV5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0200367</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKV5"/>
    <dbReference type="OMA" id="WINPPDF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19519">
        <property type="entry name" value="RecA-like_CDC48_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19528">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000004">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000001">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000003">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000048">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="6">
        <location>
            <begin position="42"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="5">
        <location>
            <begin position="141"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="252"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="525"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="782"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="799"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8053512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="820" mass="90785" checksum="52105FACB4D0381F" modified="2022-10-12" version="1">MDPKNKPQPNQPSQPNQSNQQGQSEKKDVSTAILDRKKAPNRLIAEEALQDDNTVIQLSQAKMNELKLFKGAPVLLKGKKRKETVAVPIPDKLDNEKIRLNKVIRKNLRIKLGDIVTIKPLDQVPTLTKVHVLPFDDSIEGIKGDLAQTYLIPYFKDAYRPVKKGDYFICRGGFKAVEFKIIATEPGEIGIVGPTTTLFTEGEPVKREDEEKLDEVGYDDVGGCRKQMAQIREMIELPLRHPQLFKTLGVKPPRGVLLYGPPGSGKTLIARAVANETGAFFFLINGPEIMSKMAGEAEGNLRKAFEEAEKNSPAIIFIDEIDSIAPKREKVSGEVERRVVSQLLTLMDGLKGRGQVIVIGATNRPNSIDAALRRFGRFDREIDIGVPDEVGRMEILRIHTKNMKLAEDVDLAAIAKETHGFVGADMAALCTESALQCIREKMDVIDLEDDKLDAAVLEAMAVTQEHFKFAMGQVNPSSLRETVVEVPNVKWEDIGGLEEVKKQLQEMILFPIEHPEKFHKFGMQPSKGVLFYGPPGCGKTLLAKAVASECSANFISIKGPELLTMWFGESESNVREVFDKARQASPCVLFFDELDSIAVQRGSSAGDAGGAGDRVINQLLTEMDGVSAKKSVFFIGATNRPEILDEAIIRPGRLDQLIYIPLPDEPSRLNVFQANLRKTPVASNVDLAYLAKITDGFSGADITEICQRAAKAAVRDAIEAEARQKQALQMAPNKASQLIKADPVPDLNRKHFEEALRHARKSVTNIDLQKFEEFKKKFDPSFSKGSNQGGFSFKWPEAGGQQFSRGQQSRIQEEDDLYGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLF0</accession>
    <name>A0A8S1KLF0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLF0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="193" mass="22852" checksum="0F152E6F3844BAE0" modified="2022-10-12" version="1">MKNIKLDIFNLHSIHSKHFFSKKDIYKIATIDQIGFQGLLIYKMSILIRTPGQQDFFQTFPKFSNGFICLQNFKEQFISLTSLKTIQYFFIYRVIFNPLPLDFFQQSPLQIQVLSFIFRRLFQFSKLQIIRGSINFSLFSAPLGQQLQCNWQSKSQKNSQLPKSQTYLLSTLQYLQSNILKELQKILTYINTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQE7</accession>
    <name>A0A8S1KQE7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0220358</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8055016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQE7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="71" mass="8597" checksum="D89C1D4EB1A34868" modified="2022-10-12" version="1">MLLIKDLNFNSIYKDLKNYQKQQIFGRFLFAVSFQGVFIKETQDIELIERLKIAIVLEEMDQRNKQLMIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQR8</accession>
    <name>A0A8S1KQR8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0250350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQR8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="81" mass="9762" checksum="9F92063E108E4BF6" modified="2022-10-12" version="1">MKSGFVQQIYRKIIKQILKCMNLIEQNKQKLRQYWTSIFNKKILIELTVISSQPQILIEIIKYVILLMNPIDKLSEDNYFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KR02</accession>
    <name>A0A8S1KR02_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0200271</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KR02"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="300"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8053324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="420" mass="48183" checksum="8070C39B79BA2D70" modified="2022-10-12" version="1">MVDYSDILNKKFQLQFAPVDIEELLQEVIGFYKQQIEEKGLTISYQIILDDKSKFIDGRRCQQILSNLIANAQKFTVQGGIKITVTEQQGFTIFSVEDTGFGMDQETFENLNQILENEFKSEEKISQNTAGFGLGCYLSNQIALKLSNLSILAGGGLKYIRLEKGIRVSFKIKNQPFDILYTNCDIQSMKSGLLFNENGYFDSRQSMLDLQLTNRKKSVENHLMLTVNRKSALLSISFSPNQIIYQVEQELKQENEIAERIRQCSFIKRRETQKKSPTFGASSCDIQDITNIKNFREQPHILIVDDEMINIISLKILLNQYNIKCTSAFNGLEALNKIKESNERFDLIFMDVNMPMMDGFQATEQILKLDDNFIIVACTAFTDVETKAKCYSVGMKYYINKPVTKLELLKLLNHLNLIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUD8</accession>
    <name>A0A8S1KUD8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0250132</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUD8"/>
    <dbReference type="OMA" id="HTFCQND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="64"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8056942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="138" mass="16034" checksum="1D2CCAE7A78F5744" modified="2022-10-12" version="1">MSNFQNLNNNSGDLNEYHSDDEFDFENLEIPQLVRRNGAVQSENVQGFIILDKMSDKMYKEAACPICLCEDEELYPLFCGHTFCQNDINNLLSDSELKQNIVCCPICRQYQFIESFKQLQELKITQMNTVQKDNNLTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0R7</accession>
    <name>A0A8S1L0R7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0300136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L0R7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="38" mass="4645" checksum="C3D30C1BBDBDD32F" modified="2022-10-12" version="1">MTLILHFYHVQMSFTFNRQFSYGERCCKTCNGRNLKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L148</accession>
    <name>A0A8S1L148_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0280238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L148"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8059113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="86" mass="10520" checksum="D85B9B29F99222ED" modified="2022-10-12" version="1">MEQNPLSKFKITSQVIHKIFNKTLLQQRSFWKVKINLQNKNKKVYLQVLKKNQIEIKSNKYFEKNQSSKCYKIILKSQQFVYLFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4T0</accession>
    <name>A0A8S1L4T0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0330132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4T0"/>
    <dbReference type="OMA" id="KVQMRTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8062429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="113" mass="13339" checksum="237F538F610E7A3A" modified="2022-10-12" version="1">MNPQHSKFALLKNYDFELPKMKAMTLHSFHCGRLKTTQSPEPKMPKLQQITFQSTLIVKRLQPRCRQRKVQMRTISLHKINNTTLENRTIIPLNQSMDIKGWSQKSVMEIFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L819</accession>
    <name>A0A8S1L819_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0320155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L819"/>
    <dbReference type="OMA" id="EFEPKML"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="60309" checksum="09CB41AD31C0010A" modified="2022-10-12" version="1">MIAKIVQKLRSLTMKSQIILFNVLTLVIVLSVVWINYYVQTQIFSSISFSSLYQILQKQDQDKIGFIAKNVALIIELKFLSILKQMEQIKEFYIFFEKEKENIVNNYKMDSCISIEDYINDNTSLYTPKFCYQAIGVPDYVTIPQNETEILILHDFISFLNHYSFSLDVSFSGMIQISSVSKTKYFSQFPFCFLLPWFDITSLPWYQNHLLKTQENQREGFIFSPLNDFFITKIKEMSITSSLYSDGNMIGIIREGLIISDYLIPTVPYNVLLLDQDGLVVYFNIEQLRNKTDYFYIYDQNVTGFNKTDWEDMIQFSSQRKKVILVLENKLQNEKVNVYSLQVLKNNFTLIVYTNITTKIDQQNQSTAIRETSFINFTISLFGQILLGFVAIILQIFVLLIVFKPLKQFNSIIKQYTLSQGNNVNSEIFKMIHKKTQESDALTTLQNKILNFSQILSERQGKKCDMCKIWESFTFNEKELQLDFYQIKNQFQILQNGMQEFEPKMLKIIKQGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9H3</accession>
    <name>A0A8S1L9H3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0360297</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9H3"/>
    <dbReference type="OMA" id="WDNVDIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8064780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="189" mass="22024" checksum="9C37EC4DD886C8C3" modified="2022-10-12" version="1">MAARQVYCRLQPKVNNYHNDGTGRDSYISKFNGGQMNQTLQSQLAPQANHDFEKMHYLTKSNSNLGRSGNLPPQIPIKTTKYWGDGTGRDYFVIVNDGGQCNPCYWWDNVDIQFQRQLRNYQKTGPLRISSACSSKQQDRLAEPKKQLQIKQRPHTPNIKQAHKIYQSYQTLKYQSKARNIRYKTYNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9L2</accession>
    <name>A0A8S1L9L2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0360317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9L2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="249"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="847" mass="101242" checksum="95BCB9E1AC237B2E" modified="2022-10-12" version="1">MCNIHNIKPGWLLNSKHEYQSLLVSSLKQQFSENQKIIQESSYENMSQDLCLNTEQRKQELKMYKISRVIGSSLERQVRFDKHILFQMKDHKPVKQFPLFKAISVENKVNHQNTWHDKQGDKARLSIEFEKDHSIVFIYFKEENQAFKWQRYLIIERLLQQKPKQVAKCFEYCFHEYEDKFTFIWALKFAQQLNQQAIELNKLKKQRAHMDANYFIQQEKRLMIFHINTWLQKSQKILKDEQDYQVQLQQMSLKQQQELQQNKEQLELERIQKEKLRIQMLQNLVENHIKPKKFIIQKFLTGWQQRSQFIYGTNPKKFSIKIIRMYIQNNIQLIRPLLELRFISERTEETKDIESIKILDGLQYNIQQSPYFTCRLLAQMNQQRRKDYTAWCFKDSLLGLQGKTFMTVENLGFKDYIDFQVIDTIRTNTSSKILRIQFQDLIKYLYEGRSFWIALEYEQSKIFVEMDLLEHSNIDPIFLDRLKYIQLNAVLRKVSIKMMEQRTPLPIIETVKYGLEIERYQNDIQNLYQSLYIENICLQPQCQPANWFSQLDSLEKVVKPVTDILKRNYLEFTDVTDNVQIYYQIKSIPNLRLPLLKDVDEFINKLPIVQENQHKQCRGVLISFLIYCNVRTIQLNVSFLTILFQIANKFICISHPHFLYDLSTPVFGNKFDSEAQAFWLFTGFVELMKSVHFPLVKQDTLFCPQEIVMQYMEVLHKEIVQHCKNYDIAVENVFFDILSSFYTNLVQSMPLCNIWNLIIKKISTNKCAYQLILIVYIEELSYKLLFCQNSEDFKNAISLQAQLMDIQQLEAAYSGCESNLNRIINAEQSFHESVEHSNIHQPLIQDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBC0</accession>
    <name>A0A8S1LBC0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0350106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LBC0"/>
    <dbReference type="OMA" id="IVRYKDF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="27"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="207" mass="24171" checksum="3096E7F872996087" modified="2022-10-12" version="1">MKRKPTLNAEYQDLVSQAKSMFNLSQDEMNEIIIQVKKFDPKGTGYVGKHEVDDVLRDLKLLDNDKLINKFNEELKIMNAKFLDLKQICDLYCKLKQYQQQLNEEETITQEYIDAFCALGGQLDKSGFVLKSTIIETIRKEFELNFDLDQILGEHGIQLDFDEFCQLFENAGDDAKSLLTSISLSKRNQNDFIVRYKDFEKWEKSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LF19</accession>
    <name>A0A8S1LF19_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-related protein Rab-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Small GTP-binding protein rab1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0370078</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Protein transport. Probably involved in vesicular traffic from ER to Golgi.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LF19"/>
    <dbReference type="OMA" id="FDWLIKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001018">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="180"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8065061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="211" mass="23511" checksum="9BAC1141B237C490" modified="2022-10-12" version="1">MATSIANKKDNFDWLIKILLIGDSGVGKTNVLLRFCENNFQPTYLSTIGIDFKIKSIDVEGKKIKMQIWDTAGQERFKTITQTYYKGAMGIILVYSIDDKDSFNNISNWMNQIKQHASENVCKLLIGNKIDVPNRQVSKEEGEQLAKSFGVPFFETSAKEGTNVADAFFAMAKAVKANLQNEKSPNPSVPQNGTKLIPNDVSEEKKKSGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJW0</accession>
    <name>A0A8S1LJW0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJW0"/>
    <dbReference type="OMA" id="DPFKSSQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="111"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="304"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="600" mass="70127" checksum="9F9D79E8F9CB31D4" modified="2022-10-12" version="1">MKPSRPPICSIPNQIIVYDQRLPRPNSTKNAPDTRQVIRYTTYDLYLLSHITKAFNIIYFRASSPTAITRIPHQEHIHVNQFQNLPIDSDPIQHKQATSVEYVPIQQEQQHQKYIEEINQKNQAIIELQEQISELQNKLSEKIQDYTVLKEKFDIEFKKFQEEIQLQIKGIDLNIQLNEYTSYFMQEIDRKVLNLQSNINSQIDEMKVDLESKLKQNEHFSKNLLSNTTIRLNQDEQHFQQELSILQKKLEQKMDKNMAEQIIAQSISNLQQQYQTQLSQAHQIIDKINSDKSDEINKIKIIINNQLLQVNQQAQSQIQQILEMVQDNSQSQQTAKQYSQQLKQQYNSLVSKFSQIQQQYTLKVQQKDEKLQIISKQNEELKKEIQDSQLIIQKQQQELYKISSNNKFQKSPYVQQILSPKNIKSNSFYTNSTSAGLNFTKNRPLSVSKKSKKQHNSNPFEISIEDFANFNENSILDSNQSIELLTQNGKSSLIQSPKNPFKSSQLMNINRFLNENNTKIPFIESTIKKQSENIPPLHQAIKRRLLYLGKQQKTLLCTYSGEQLCDAEMGMQLLDDNGKPFIINEDEKNELIAKQLIQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LLG3</accession>
    <name>A0A8S1LLG3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytidyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0400209</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LLG3"/>
    <dbReference type="OMA" id="VYPAPWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004105">
        <property type="term" value="F:choline-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045049">
        <property type="entry name" value="Pcy1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739:SF13">
        <property type="entry name" value="CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739">
        <property type="entry name" value="CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="3">
        <location>
            <begin position="11"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8067295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="186" mass="22164" checksum="5D267FD397C958B4" modified="2022-10-12" version="1">MNKEEKQVRVYADGVYDMFHYGHARQLEQCKKLFPNTYLIVGVCSQEDVEKFKGKSVMDGYQRTESVKHCKWADEVIYPAPWIIDEKFLNDHRIDYVAHDDIPYTTADVDDAYALCKKLGKFKATKRTEGISTTDIIGKILKDRHKYLKRNIERGMSRQELGMGLWEYYQLKYDWLFCQRRKRKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LLS4</accession>
    <name>A0A8S1LLS4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH-ubiquinone oxidoreductase 21kDa subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0430072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8068900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LLS4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053229">
        <property type="entry name" value="NADH-Q_oxidrdct_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019721">
        <property type="entry name" value="NADH-UbQ_OxRdtase_su21_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34062:SF1">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 21KDA SUBUNIT N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34062">
        <property type="entry name" value="OXIDOREDUCTASE 21 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04750)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10785">
        <property type="entry name" value="NADH-u_ox-rdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 21kDa subunit N-terminal" evidence="2">
        <location>
            <begin position="35"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8068900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="151" mass="17551" checksum="38E5310DBF33A747" modified="2022-10-12" version="1">MSTYRGTFEHDSFLGWLNLLKIRRLQVLYNVGERPPYPVIISKPTVGDVLRNLNKADFGLFATVAFLGFFAARRATLGLTTTEYIRQRGFSIAWNSIMMAGALFACMNSNNRLTGFVDNGLQWRRKEQRLTKYDFTSEFEEGTIWKFFRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LNP1</accession>
    <name>A0A8S1LNP1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0410258</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LNP1"/>
    <dbReference type="OMA" id="TYKGFMA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="26053" checksum="718EAB1E53D6D3B2" modified="2022-10-12" version="1">MKGILSRQSSLSNINHNVNQALGISSQYDFEKGILVNVPVDLPEVKKNSHQKLPRLMRKDQKLQKFFVLNPKQQIKSNDRSKTKRLIPYQLIKVEHPPIGYYKLNESSLVNKSVVDMSKQPTARIPIRKYKSKSIDQEPQIPLPQLLDEPKKIQKPRITELSNLKLQEWIQKELEQLKDAKKLQQTTYKGFMANEKEKSDNFQRLFELTSLKLKNSYVKYFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LRN1</accession>
    <name>A0A8S1LRN1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LRN1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="165" mass="19214" checksum="E625DD3A2B803B4F" modified="2022-10-12" version="1">MCNKPQEKKSVYYHGKDKNRSGRIISTNEPPLLQNQVSQKFSQFSNEYERRDCCINLLCRIANLVIQLIWKNLQFLQLNNNTLCYLDNKFTFNFQKTCSYIQAIIVVCKLSDEAKLTVQVLNQTLQSAQVNNVFSIKHVFIQHLKALCYATSFKCRAYSINKTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LY98</accession>
    <name>A0A8S1LY98_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0460223</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LY98"/>
    <dbReference type="OMA" id="MIQADNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000116">
        <property type="entry name" value="Cathepsin D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000104">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035804649" description="Peptidase A1 domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="7">
        <location>
            <begin position="89"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="118"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="279"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8070955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="414" mass="46799" checksum="F9694C73A7C1DD0A" modified="2022-10-12" version="1">MNQFLFSLLVVITLCGQQQGNFLSEKQENQIFTIKLKETQKIVTAKEMYDFLSTKQTYFQFGGYVPKLQQNEQTKKTSLKLHNFRNTQFTGPITVGDQEFQVIFDTGSANFWIDSVKCKNEGCKQHTQYKPSLKSKHLGYALNVEFGTGDLNGEINSDIVRLGEIEIEDQNIAEIIEENGSVFQNTGFDGIVGLGYPSMAAYNFNPLFDNIIKEKKLKSNQFSFYMSNQVNSYESQITFGGYDVTKLDGQVHYHPVIDQYYWMIQADNILINGKDQGFCPKGCKIIADTGTSLITGPFEDLMKLLDLTNINDDCSNLNELPKLTFRIDGVDYDLDSKDYIMELRDDGTEIPINTDVSASAFIQGNSDRCIGAFMPLDIPDPQGPAWILGDIFLTKYLSIYDRDTNMVGFGKAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3B6</accession>
    <name>A0A8S1M3B6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0530195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3B6"/>
    <dbReference type="OMA" id="KINFFWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="42450" checksum="8E6453362D3D23D5" modified="2022-10-12" version="1">MINYQNYAQYNNTQNNQNNTQNLNPQPQQNGYQFSNQNIHNQNMYQQNMVQPANYPKLNFEKESFSEDQPDHIIHHDYLQQQQVTQQQEPYQQYNNNDNQTQYIYVNQQIATPFIQPENENNQDIMTEHLRRNLIYKINFFWIIQQTVQIILCIINMASYSYDNLFFDYSLVQYRITIYIFLFLSFGYMLVIRFIKPLWRIRGYQSIIYIIYTILYSFFISGIVSSSHLSFYKNKMYFLIGFLYFIGTIGILIMLIQFQFQIKHFKEEDMKLKEFIKGVILGPIGILFLITAIFGVDAVSMVLCYELIWSYIFTLFYINSLLQIYRGKFKLKRDQHFVGVILVYINMFIPCIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3T0</accession>
    <name>A0A8S1M3T0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0490145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3T0"/>
    <dbReference type="OMA" id="RINGAQK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="109"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="330"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="479"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="507" mass="59982" checksum="BFA3142F603C2AA3" modified="2022-10-12" version="1">MSQSWKMNASIETVLLKDKRSATSQLVQMKPNDVVAQELYDLYNYFQEIQKQNKNLQDSITTLRSAITNLLSLSGRKCQEGEFDKVCLNDYKIIQSIMNRVRVDIQQSEDKSNNQSNSQQNQIVDLQNKIYFLEDQIEQLTTKCNKLIVVNNQLFQQNYEQINNLKQLQQKQIEQNKKTSQILLENQLQSVNQNSINIEIDCQQFREKIIDINNQRQQLIELMQVTLEKFPQIQSDKKVQQNIKNQSFDGDDLQNIINLIRKSQNAIKSYDDGITRLIKQVNSVVQNKENKTIQDLLQQLSKNQECFIYIQNIFQRIIEMLNQMTQVQKTNELEGQLRKMQDENSDIKQQLQDQIKQLKEYNKKEQQLLPNQMINISNKFNNIFKEFNDQVKKEIYKFSNIIKILLPNVTDVSNISPDQDLLHDIQSFIKSIESQDINQDDDTQKQKWLRSINLFKEIYKRIPNNSIVNQYIIKSVDSIEKFFFQIEEYEQNVDSFKKEIKMSAQTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M469</accession>
    <name>A0A8S1M469_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0510031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M469"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="114" mass="12979" checksum="9FE34C5A577C3903" modified="2022-10-12" version="1">MQQHCNLILNERGTLWLGDYESALNLEFLKSKGIRTVITVAAGLNLKYEGIVHHKIEILDIELTNISQYFQTANDWIERGFNIGGVLGSINLLQEVKFIAWQVSPDLQLQQLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5S1</accession>
    <name>A0A8S1M5S1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0500223</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5S1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="304"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="577"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8073131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="709" mass="83054" checksum="EB49F03F7D14D409" modified="2022-10-12" version="1">MIQILVTYTLHIEIQIPQFSFAHSLFYAYLMFKNQIEFWILSINILFIGLYCILRQYDYLDQQVIWSTIHYAIVYIILTILNYDVFKQQNLQSSPKLIELISTSERYRWKEMSQCELFQDNYTKNMCFNQIREGVILYEQGYEKPPTFINRSAKQMFQISDENQIQIAYSNYCRIERMEPKRSIQSIKSIGIDMQNSQKQYSESLSFNSKQQSQYIPQQGLKEITLLQMLDKIWQKQNKESYLFYVQQSKHLTQSQQSPILEVSVYLNSQVRKVMTLICRDLSYKKYIKQLQSHYYQKSKMISFVSHEFRAPLGCMITMLEHVAKEQNNLYIQNSIDNSKYLLNLCNDLLDLAQIQANKFQLKKERFNLKMLCQECLQMFNLQAEKKQLKLMLQYDSSCPDIIEQDQNRIKQVLINLIGNAFKFTQEGQILLRVELIDELVIEISISDSGIGVQEQDKEMLMKAFGKINSEESKKLNAQGVGLGLVISNKIAQQLGDGLRFESIYHKGSSFSFTIHLDNIQSYESEIIKSVNKKQSTLRDSEMMENLYEDGESSKEIQIIVTQPKKLMNSTVECCSKVLIVDDTQFNIDTLQMLLIRIGINQIDYSINGYKAIEKVKLKEKCNQCNSNMYQLIFMDLEMLGINGINASKLIFSYCEEKNIKPPIIVACSGYQKDIEFPKCQQVGMKFYLEKPVDIRQLQSIIDYYRSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9J5</accession>
    <name>A0A8S1M9J5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0500296</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9J5"/>
    <dbReference type="OMA" id="VLIHKQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8073276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="510" mass="60605" checksum="97703816797EB926" modified="2022-10-12" version="1">MYQTETKSSLYASKLIQQTNYSALSSLPKTYIRQVQAEGEIFMAAPQKCAKIWKEFRRNDFTQVQIPYKTALQVFDACKEDFQMMLKISSGKDFFDLFDQDNDGYLNEDEQILVFSIIKEKMQQVANLLLQIQQYVPFKQLMKAIRTLEQNICEYQDILRQKIYKSEVSTYKEIGFEKLDDFYEKYYNYFQQFENQKKIRIQMQIQKQSEEMGQLEDKLSKNTEFLKVKPKRKLKDLQTQEKLVSLDERVEEAMDFRRELKNMEKAEQERVASEQEKRIESQKDELQRKHQKQMEQLKAKLIEQEHKLIIQLKKEYNVLLKQIGLHSNEIDRIQSSATQNAIRKGEKEGELKRMKERARIQNFIIGETKRTMTPKPQQLNTSTYEDTVSSSPRSPAVLSKYDRAAHEIKVLIHKQNYTSFYIKKKYGADLPVNYKPEPYKLQGDNHDRIEKILSVKKKNPHDVLPSLTELYDENLKEQDKGSTQKSEQELVQERLKKKQFILEKLEESTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAY0</accession>
    <name>A0A8S1MAY0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0580178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YTIVRTH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1032"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1181"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1286"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1496"/>
            <end position="1517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1561" mass="185172" checksum="C1DC78009D4DF086" modified="2022-10-12" version="1">MKRFLLKIKAFYIKDFLSIKHSKKITFQLAVVMIFFENLQMISVYTQSLNTISYDEFPNYARSLINYFNIYTLFGNSITTRIICVMVGFSIKSILLLKMLCLVSSQDLFLQQKLTLHDIPLDQKKQNYHFEECWQSNLLSWYIQINLEVLQIPILFCAISLVSDLISLNNLNGFEYLGLLLSFILIIQQLLLGTLLHFHQIEYRFKNYHLLSKQQGLKNDILYILRLFIIFLSGSLSNTIAIQTLGLIINSIQTVFTFKQLTYIDHRILSISFKINSLMLLYQIALIICEFGQQKIKISLSLLILFFYPLILYLIQQLSIRQEWKNDLKDQQQFLRRVYWNVKQQVQFKKRQQNQDQQRLFQIYTIVRTHLQECKQMRMRKILKNTSFQQKCFCQTFSNQKDQIQNIEKCKEFLKLLLGQELEDQIKEEGSIQKTLTYICYLNDIKKAPISAIYEVIRISTVEKLSLRFQQIIHQLQQNSQQRFNQLIKKQNLVNQIFDFKKACLFEESIKILKLNFFLIVKQQIEFYEVLFSPIINAKIIENKGLSLVTNINDLENQIQLIFRTNPQNGECDTIYKLFQKYLNYNKSRTKQYRREGSLTAEFIQSVDKIIYNQNSCVVQITLLQPRGNVIRYTRSFQQVIKYKDDEILNQNIKKFIPSIIANDHDQYLNNFVETGRINVLKRELRIILVKLKSEFVIPINTRLRIEVNPLEFGAQALMTPVNQTYGYLMLNEKGQIEEITQNIYEDIFKQYLGLDLDSVKGLDFLLMIPELSKIWESLFDENFDKLDLYQEGQLMIPLIEKQTISPINSPNYGNKQLLNKRLIEYMKKYSQSILKYQINFHLTSLTTINLRVVIVEIPEYRQTINKQYASQVELIQAKPTKSQINITYQTELLTHKSPLNTPPPSKTLMNECDLEYDNLIEEKKMIEEFRNQLASLTMNNTIQNTYSKEPIIQQQQCQNSTDIKIIMPSNVLSYKSEEYEQRQFQQEHQIQQQYNGSVGSRSSQNNSNTIRQQIKNCLQNNKGLSFRIKTFLFIFYTLLLSGFILNFCILLINFQTVDANVNFESLPYQLSYYYNEFVIAKVYQSQNEFEFNKLLGVSINYYQQHQKYVDEIVSLMPQIQIINDQTMQRRIINLISQMSEVYHNSNSTISLEDYLNNTYAFHILLDQFYGANSPLQIQSLLNYFILEEIILFLLLFSYAYFYVSILKIKIKFYKLFCTFSRDFIKDQFIQFHSLYNQINHSKFKTNETNEESFEASMISQFQKNSTQSNLESQVRIGKQIQTPQISLNLFIFFFTMLIFSVFYTFYYFGTYMLYKEVINNIEDNNWKRIYYENIANDLIFAYSQQALYYNQTINEEMIQYQNKVLSEFKSRISQISLPQELQNILIDRILLDEVCSVYQEGIILQESDYQDLFTQEQCLSIPSLSKGLIYAVQELFYNYNDFVEDIQSKYYNQISKISFLLDVNTQIKRLYSQFSFQIIIEILRKEIKNFISYTIYLNTLTFILANFLAGMTILIAKFSIEKAYYQYQDNKKILSLFPYEKLMENAYVINFIAQDLNFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MHE9</accession>
    <name>A0A8S1MHE9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0590004</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MHE9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="220"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="254"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="288"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="322"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="356"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8077831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="47436" checksum="0DA102ED3D4AD5B4" modified="2022-10-12" version="1">MKVFKCQFVDHEDESIMGFCINKDCQKSTQFCFKCLTKVHQDHPNDCLRFDNLSTVIKEHIKTHTNLITQYHQIKSQLVQVFEKNQKKLEQYIQILKQIDSELQNQSYQFVKQQIPLLKLLYSKDSYEKQNSFIKELNKTIKTFETLTIDDIKIQEISDNSTHLKQQDSIETQKKQKAQELQLKDEQKRQKIVGKQLLDYEQDDEACKFQPNSSKDGISAGNYLLKGNSLLNQNNYEEAIQCFDDAIKINPNNCLAFNNKGNALYQLNRFEEAIKCHDQALTINPNNCLAFNNKGDALYKLNRFEEAIKCHDSALNINPQDCDVLNKKGNSLQNLYRFQEAIKCYDKILTINPNNFLSFHNKGNALYKLNRFEEAIKCYDNALKINPQDKEAQTKRLSALGMLKKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MHR0</accession>
    <name>A0A8S1MHR0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0570268</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8077193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MHR0"/>
    <dbReference type="OMA" id="AACYFNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051616">
        <property type="entry name" value="Cul2-RING_E3_ligase_SR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46224">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46224:SF6">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="61"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="127"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="228"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="386"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8077193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="50533" checksum="106E9FDE4CEADD64" modified="2022-10-12" version="1">MSDIKEIEVEKKFIEEYFKQISTSDIDTIKRYLLYVKQRKRGNENEKVPIRTFINVISEGKGKKAIHFGASRGDLDVFKFLVKKGADVNLLDDEKNNPFMIAVQHNHLSIVQYLIDFHHVDVNYTRNTITALHLAAQQGAIPMIELLLSAGANINSLSNFGTPVSFAVAYQQNWSALHLIKKGADLNIVQNGMPSLLHLLIDQNNQELFNTIFEEFPDKIDVNIKDPDGWSALHLCAEKGLLKFSQTLIKYGADINYENQKQTPLDLAVQNEKWDCVEYYREFALRKEPQIPPSLVQLTDAQVKDLYNEINVEKVEANKLLQAEKYEEAIIKYTEIIEKSKQIVDQYRDEIVKIYTNLANAQLKLSKYKDILTICKQARNVKKDWVKIYYYEGQAYEGLNEFGEAAASYFEALKIQQEPGMKALFDQAIKRGKELHKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKS1</accession>
    <name>A0A8S1MKS1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0630132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKS1"/>
    <dbReference type="OMA" id="YLYINPW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="249" mass="29230" checksum="80110F022008FB3A" modified="2022-10-12" version="1">MLIRRVLYRFSKEKINKILDHEDYLTEQIDKMAAAFNPQNNKIFNNYKVFQNKLNVDGFSTKLNRSASIYITIWSMVGGLSYLYINPWVTLIPVFFSIQTISSYMKGRKYLGKLVQSITLEKDQKKCLIELGNKNVLKVNVTDNELLNISDVIQVSNNKIEEEVNEKYRQSNYVIQFETKYDSKLYEDLRVLVTKDNAEIPNIKLLQDIITGEDVSGYIYEEIVQNKEDVVRKETDEELEKRLKDELKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKX0</accession>
    <name>A0A8S1MKX0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0640231</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CIESNQK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="245"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="279"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="381"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="415"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="486"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="588"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="622"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="656"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8080920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="705" mass="82770" checksum="85A80F7D1D61A613" modified="2022-10-12" version="1">MNQKFQQKQDFKCKDHPQTSAIFWCKSIDCRENRIFCINCQKQQKHYQHFIDDVDDINQLNQFLITKSKQSKDLITECQLQYKSTVQSYEQLISGLNYKFCIQEDKINEFQPNQALTALDSLVLFDDMKIHIKSNIIGPLQKIQKILEDLYIQLELQTIKYQISDQQRATNQIEYQKGSFFYIYFFQLSLQIQQINQMMQFKYLINYYQLIPLIQNLSNSLNKLNKQQEAQQIYTNIIKINPQYELSYWKIGDILREQKSYQKAKDYYDKCIQINNNNSASQFGIGECLKETYKYNQALEYYIKAIQLSQSNQLYIQSYGDCLRELERYQEAIQAYDQILNNQSKNADASAGKGICLLYLNMYKEAEVLLKNSLTLKLDCILGYYGYGLYLYSQDQYQDAINYFVKALDINPQHIHSLNGKGDCLRLMNDYQQALEWYQKALDINPQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGLKETYKYNQALEYYIKAIQLSQSNQLYIQSYGDCLRELERYQEAIQAYDIILNNQSKNADASAGKGICLLYLNMYKEAEVLLKNSLTLKLDCILGYFGYGLYLYSQDQYQDAINYFVKALDINPQHIHSLYGKGECLRGMNDYQQALEWYQKALDINPQYVYSLNGKGECLRGMNDYQQALEWYQKALDINPQYVYSLNGKGKFNSINQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MMV8</accession>
    <name>A0A8S1MMV8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0640089</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MMV8"/>
    <dbReference type="OMA" id="PRIKFDW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8080662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="40041" checksum="1B6C50A5426337AA" modified="2022-10-12" version="1">MATNEDKQYFKTGVVGFVFMYSMFKFGHYFNNNRKTKNQIKKMKQYPIKNVSEVAKEVQNQQKSNIYFIEGIPQKGSLYSTFKKIPVILRIKQRTSLFSCDSFLEPRIKFDWKEASSSSDYQIDKPLYFGLKDKQNQTEALVSINDETDITTALMSFGFKDEKRSLSFAEHIGNYATIFYSSLFRILGPAALIQVLFRDGSFRGVHIGYRDNEFGIKTDGFLSVLGQAVYNTETKKITIENPLALTLSKSNFIYQLEKKLQENGVAFKFWCTLSFLSGLYISRRVFLFHKKHPKILKKVIALFKKLFKRKQKREENNINNEIAKPLDNIPDEKKAEANETPTELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSI9</accession>
    <name>A0A8S1MSI9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0670171</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSI9"/>
    <dbReference type="OMA" id="NGTKHGN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8082272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="40583" checksum="578644436CA00DC3" modified="2022-10-12" version="1">MGICKSLPASTEDLPTMDSSGHKQTEIVIKEDTSPIYDENKIIVIQALYRGHKVRKSLMTTLHSPETSAPTQVEQSKFCFDPNQLNTYDQSSILMKNGAIYKGEWKDGKANGKGQYQFQDRFYVEGTWASNELQGEAVYVNGTEKYKGQWLDSMFHGIGEYVYSDGRIYQGEWKKGLQHGMGKEIYKDKSTYEGKFKEGMKYGLGIMRLADGCVYEGEFENDQFHGYGSFIWPDRKKIFEGYWKNGTKHGNGTMKWGDGMRQILIQLIFKVESILVNIQKDLNMDMEKCNIVMVAVIRDNGRMDYKMEWEYLQTRKGMKKKDSGLKENQRNGFELLQSFDFQSLLLLLYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSX9</accession>
    <name>A0A8S1MSX9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0630129</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSX9"/>
    <dbReference type="OMA" id="FEYENTG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12232">
        <property type="entry name" value="RRM3_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001286">
        <property type="entry name" value="U2 snRNP auxiliary factor large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="203"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8080173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="435" mass="50874" checksum="B14D31906067DA1D" modified="2022-10-12" version="1">MNQEVENKEIKEQKEQIEQESDDNEKNEKKEQTQQQEQKKKKKKNKKKKKWGEEEEIMTDFDDDWQNKVKLSKILQDPDLPQHIKEKYAIYENNPYMMIVSNVPLNVQLKELEEYFNTLITSLDPKITERPIKAIEYGATKSWVVLECSSKEAKRALVTQDQVQFVNNCKIKVERPRKFLERILNPQAKEGELNAEQKQEDNTRLYLGGLPTYLRDEDVMKLIQSFGTTKYFNLVKDTTSNTEISKGYCFFEFENTVSTAKALKALNNLQIGDKKLKICKVQGEPQQNKKINGRDQPSNYAGSFLASCDLLRMPQVQQMLTIPQSALIPSKVVQFLNMCSIEDLYEDDLYEELMEDIRSECIRYGQIEKIEIPRPDKESGFCNPAVGKIFVKFYYQIPAKKAKFHLAGRTYNKRTVITSFYPEEQFDYKDYLING</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSY6</accession>
    <name>A0A8S1MSY6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cathepsin L</fullName>
            <ecNumber evidence="10">3.4.22.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0710210</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in extracellular digestion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.</text>
            <dbReference type="EC" id="3.4.22.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.15" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSY6"/>
    <dbReference type="OMA" id="PKTHFRY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000104">
        <property type="entry name" value="Cathepsin L 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035820787" description="cathepsin L" evidence="12">
        <location>
            <begin position="24"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="36"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="119"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8084167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="327" mass="36503" checksum="D8A4CC5E1100D7DF" modified="2022-10-12" version="1">MEKTLLAVGLMMLLGASIYLNNTQELSDEIDTANLYTNWKMKYNKRYTNQRDEMYRFKAFTDNLNYIRAFNQSPEDKTYTLELNQFADMSQQEFASTYLNLRVPKTAKVNAANDNFQYKGAEIDWTDGSQIKYPAIKNQGSCGSCWAFAAVATLEINALIELSRKYDLSEQDLVDCSGPYDNQGCNGGWMDSAYEYVADNGLAEAKDYPYTAKDGVCKTSVKRSFKHVVGFKDIDSCNELAETIQDRVVAVAVDANPWQFYRSGVLSKCTKNLNHGVVVVGVLADGVWKIRNSWGATWGEAGHIRLAGGDTCGICAAPSFPVLEQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWM9</accession>
    <name>A0A8S1MWM9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0610228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWM9"/>
    <dbReference type="OMA" id="FELMPNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="413"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1107" mass="131305" checksum="00DCD3DA498909F7" modified="2022-10-12" version="1">MSLSNQIYSLLRTQETYKQIKQYVERVGNKDQAVYQPICDAIIQALKIKEPVLNLMASRLGKEAVDQRNVEFNTLFSEICLPYYEKIAEFKKDDHSDDRGKHYFSENPDKNQSSYGNSFLRLILESIEAWAKIYTCKSDQVTYTPFFLSYARLVVKKIKFPDEFKYYKKEEVDKNTTSIFKTHSKNIPQVLEDIKSIPKSQPSQLNQQDQPYKQVQPTQPIQVQKIQNIPTPDNDTQKEINQSAQLLINLYSQLKTQLIEKALSLDFKATGQYIKDLNQVNQSLYHLMEKIDIKVEELLSQDDEDSSLELLNVQDEIKILCQYFQKLQKDELTPQQYRNKFEKYKQPVPKQQRGNLQSIYEETENSQSSMQFGKVDKKFVPNTNIDSLGLQYNAEQIMQDFQIQLSGITEQYEKEKQLRQQEHEQEVTNLKRQIQEINQVKNIQENQIQELKKKEIRYKEIEQQYIDIQKQKNNSSQSSDEVKKINTTLTDQIKALKLQNEQLQNQKEKDRNDIDKMRTHIADLKEENDQQKTEIEKLKKELYIEKNQITSRLQQQEEYYKQEKRQLQEQIKFSSSYIDNHNLDKTPSKEISDLQKRLENEKTRSANLIHEVNNKSEQIKIFEQELKNKQIELVQLRAEQNIQFTALDIQVQQKHQAEIQNYKQLLLSLETSKKIEENSFKEKLNLLEQKLKISFSENEKLRNESSILKNKIQELNQQLEQQIIQQKQDQIQIESQQQLFNNKLDLSKIKETGFEYVQVQSPESISSKQNFSQAIYPVSAKKQEKPLTSIPYSGYQHFQNLQVDFIENIKNLFIRTQQIKVLVQSDIIYPYLTAEEYYEKKIQPHKGIVIQFKRIIPNLVGNQNNLILYKMYHLNKINNTIFVDNQDLKIAAQKYIQKNGSNYYINYGLYFKTDLQNLNLKAYMKNFMNFETIWASQLEIQQNLQKKQQILLEVSIKLQQSEIFETPLLEVNYNNKIQQVLLPLKILSMIQYHRISKIGYQRKWKNSKIYRSEVFEYNTLILSNHKDITKLNESFSMTNPEKIDSYIQGLDEIKYFAQFSLQYYNIDGIIKFELMPNQKMIIFIGLENNKQNSLIQKLINAFQDIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MY31</accession>
    <name>A0A8S1MY31_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0620266</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IFT46 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MY31"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022088">
        <property type="entry name" value="Intraflagellar_transp_cmplxB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13376">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 46 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13376:SF0">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 46 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12317">
        <property type="entry name" value="IFT46_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8079914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="217" mass="25000" checksum="2D0ABD81D963917A" modified="2022-10-12" version="1">MQKNNYGFGVDEKAKKIDNEHSDEAIELKDENDKKLVVVKKMKTNVTCLPTTLLDQAGARGAKQAAQSVLFQEGKVSLSAGAQNPNDYSNLQVGAEVKQYFEFISRYFYFSFKIQLYKVPQMVLDAKLKPFIPDYLPETLVLVALDESALNQSKRAKFDLLMRENVKVTKRAKILLFMLQKMLITTQRKLQHVLMMQQKYIKKSNLHLFHILNQACY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MYI5</accession>
    <name>A0A8S1MYI5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine Rich Repeat family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MYI5"/>
    <dbReference type="OMA" id="NTMQIIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1091" mass="127087" checksum="007955AC5B528BD3" modified="2022-10-12" version="1">MCNKFKAINMDDTSLGSIDSKSVMFLTASPSQFKTISQVSLSRKISKLEPLIVEKDKRNDSRSQERGLLKDLGVSQVLDWKSFMEKDAEQIKEFNETTQKFRRSSIQSQNSQIFLSPQKTDKQNPSQFEGRRSSINLKTFDSDDFLEKMKLGNKQLPNLTTTEFMKCVRQKEKLTEYAKVYQQSPENLYLKLNNSQNFVPKIFGLVSPLGSPLTQVNASRYLRNIDDCRVYAEAMATTQSQGIQKMQLNSNIYEPRQFKELLISFPNTVRELELKDCKLNFKHVDTLMAYINKNLILKLNLEQNLLRDQGCNMLIKHLMNNNTLQCLNLCNNKITESSSAALSNFLKQSQRLLELYLGFNNLQINGSIQIWKAMYKNTSIKILDMSHNAIASLECAQAIAKALSRPYNELVHIDLRYNKFNQQQSEIIAEGLIKNETIYGFHFEGNYQDIIVNPNGFLINRREEIKQKQKKIDLLNKQQQYFKLLDEEKIESSNNLKTDDDLCLAYHRSRRMKSTELNKQKINMINKLDVCWICEGWQEIKFTWNPKSGSLKTEPIFIHFDFDDYKPYLMTFLNGEFFFVKMCPPNRSIKYFFTNPILGIQCIAEDQSVLSLNQPLPSIPFLYNNEILVDGNTMQIINELISPNNQNLFDRYVPLVQCKPREKMALFDFSPYLNIASTKWSVETSIFRYFPPDTNKLIEECFEFDYQNSKINRLVKETELQEVKEILKQFYFQLFNCYKYFASGNPTAPIPCLGPTDYVEFLISNEIMEGVKPNDIDIGFTSTAGAKDVSFPQAYEKGIVRCQLMEVLVRFCNDKYIRSGLCQTMTEGLSSLQQQCNEFLSKFDSSQSWRKMRLWNEKCDILIHDRMPMIKCLYKYTCKLSKKPQQYKYDYVSSQDFKDLLKQSNVICDELTDRECYLAYLQSMITQKDELFQPKHYQMTLYEFIEALARIAEKVSIIRGEKVLEIENRRAQDLQDKISGLLLLMYLTLIDEIKKALPNEPDVKHLEKCMNNDFRSKKQKLEDSFTDGDEPPYDVKVELPILIAQVPNLQGNLNGPAKRMTIRNIKLLKPSTTKYSLTNFVQFFISLNERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N086</accession>
    <name>A0A8S1N086_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0750187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8086020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N086"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035924206" description="TNFR-Cys domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="25782" checksum="42646F4DBAF31EDF" modified="2022-10-12" version="1">MKQLIINLLIRFIPIIGVVGLREYSQWIEVFGNANGILCPELHTYIPDMHPHQCVSYATICKGVTEAEIKTEVNGTQYVCHPRFQPFDEGTPTKGDYSFHCGSDFGQTLIMERSLDGHYKFYCKENLYPKTCLIQDKQRCQYCKFPFFGFECESVPQYYDRTQTNPVRKCDGQCEECDQNKVCSACRFDYEKNNSTDNLCSKSKMLWFSTLCTNQKRQFEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1E2</accession>
    <name>A0A8S1N1E2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0770011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1E2"/>
    <dbReference type="OMA" id="GSAQCCT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="194" mass="22183" checksum="006B2AE69DAB1838" modified="2022-10-12" version="1">MGSAQCCTKQQLEEKETEIQIKQDVIMKQSGTQQDNPLPKNKLSSRKSSPDEPGTPGFADLRSQQSRKSEQHQSHSQKEIQLIGQLEKHDSPFKSQCSIGKRKNDSPVCSFEATNKLKSLDSNRIKKIQTFEEKVNSRDLSPIEDDGMLDSQRSKQADEKSVKSILKQELKYSKFRKQDTTFDAGQRKVQFYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1I3</accession>
    <name>A0A8S1N1I3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0760192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1I3"/>
    <dbReference type="OMA" id="RCITIPD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="644" mass="77795" checksum="0941C1CAFACB0E5D" modified="2022-10-12" version="1">MQFLSQKFVSEKFEGQFQKYLKKFRIRFQLIQKVIILPLIIYYTIEKVFSSSWFVVSLNIILGLIVIFSLKFQKKCIRVYEILILIGILLFNLFYATSQYLNVHPERYQFIDGYFLAILSLTMINMMDTIQKAFLLLFIYLIYLIMIPNKAEPIWQQIVKFFLYIILYYQQQRYQQQLLRLTYLQYFKHLTIENTIRENLDMKYYVVHFIETKRQFLLKSEDYNQYLNEEDQYAFQQYLRKINISSSSQDIEKQTSTKMRSAKMNLEQFLFYLFTDRKKLQSLQSYENKYLEHQHHIFGFTTGESYIIKVIKCYDTEPCAILLITEQQKQQFVDKLKLQNKATLKLLNYFSDIFTAQIRVALIVLNRMLKYQQKNKKNVIKNKEYSFLKYINSQLYIAYNQFYNISDYFQANSEFRRIQITRFNLIQVIEELFEKLKYYRKIDQIMTRNFVLKTKIQELYIKSDMKQISQLFFNLTKFAMQYSDEIQIDLDEGFDQSYPPQPIINVQILFKNSTGTKIELRKFPIINPKTLQEIKTNDKRPLDLDVSISLLIIRNLGPFDKMTIRKTSKQFYKIQFFIYKSMNQDLHLIPIHSFDPSLFIKRDDDWQQLNHFGNQSVINCYNDSPEIRLDTLRCITIPDTALRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7C4</accession>
    <name>A0A8S1N7C4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0810073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7C4"/>
    <dbReference type="OMA" id="YANGDSF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="32024" checksum="8967A21517ADA9AC" modified="2022-10-12" version="1">MFNIQSNEQIQQEIISKISHLQLSETDDDNSRQSSPALDSRLLEIIRELPQHLKQKLGSLIPKTNYQLIKQNDDSIYYGIMENLQKQGEGIQIWSKAGNLLEGQWENDQLEGYCRMNYANGDSFECQFKQGRTNGFGIFKSQKKTVKGLWIDNAIQGEGQEIKSDGTKYFGQFYNGKKEGRGILILNDGCKYEGQFKNNLFHGEGTFLWNDGSNYTGTFTKGLILGFGQYLNGNGIQMIGQFTELKQPRGCNEEKNQHLQTIVFVNQYNQSLMIEKIRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7F4</accession>
    <name>A0A8S1N7F4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="18">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PPRIM_AZ9-3.1.T0810123</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Na(+)(in) + ATP + H2O = Na(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:14634"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="18"/>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7F4"/>
    <dbReference type="OMA" id="SDHVHVN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008554">
        <property type="term" value="F:P-type sodium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000061">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000045">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000191">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 18" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0915">Sodium</keyword>
    <keyword evidence="15" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="340"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="791"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="824"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="863"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="906"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="947"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="977"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="43"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1034"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1043"/>
            <end position="1058"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAD8088520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1058" mass="118537" checksum="DC2C44C1C33AC416" modified="2022-10-12" version="1">MKQQYSKQENSKDFEEFHNIEELKELFLLNSINDGTSFQKVIKLGGDQGLAKQLKSHLLKGIDSEAQVQENREKYGNNDPIEKEPAQLCELILECFGDTMLQILLVAALVSTVIGIINEGVKTGWTEGATIFLAVFLIVSITAGNNYLKERQFQQLRRKLDDGMVQVVRGGIVEISIKEIVVGDILQFGIGDIFQVDGLMIQGSQIKVDESPMTGESDEIKKLPFNEMSQQQQSSKDHHHFSPFLISGTRCLDGNGYMLVLQVGQNTVQGQLKLLLNQDNPPTPLQQKLEGVAENIGKLGTLVAILTFIALMGHLIYDVFVDHKHEFFTLLSLQLIIEAFMIGVTIIVVAVPEGLPLAVTIALAYSVGKMKDEQNLVKNLASCEIMGGANNICSDKTGTLTQNIMQVTALWIENHTYNNQEINITSKISKQSIEIMSESICYNSIANPTKDRDTNRWTQIGNKTECALIELADNFGYKYSNFRQNDRILRQIPFSSKRKKMVTAILNPKNQSIRIFSKGASEIILGQCFRYISNNGAEQILDKTKKDDILHNVIENFASRSLRTIAIAYKDLEPQSQAIKGFVNAKAHVHQINEDEIDKDLTLIAIAGIKDPIRPDVAESIRQCTKSGVTVRMVTGDNIITAQSIALECGILEKNRAQQEFEVIEGKRFRELVGGLVNGKNEEGNEIKVVKNMQIFSKISKEMKVMARASPEDKYLLVTGLIQEGNVVAVTGDGTNDAPALKKADVGFAMGITGSDVAKDAADIILIDDNFSSIITAMKWGRNIYDCIRKFIQFQLTVNLVALFMSFTGAVILKQSPLNAIEMLWVNLIMDTFASLALATEPPSIKVLDRQPYKRSDQIVSPTMYRTIVGASLYQIMVLTFILFLLPKFIDCSIPQELIGQKYPKNVVQMSIFFQAFVLMQVFNSISCRQLDYQTRNPFANFCNNPLFWIVQTITVIVQILLIQYGGKYVKVSHLTLFQHLLCVGFGIGGIIFSILFKFIPEQLCQKIHLFREEEIKTEKMDDTLTSKLRRKSTMRLHTSQRSKHDLGSLKKMSSDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9Z4</accession>
    <name>A0A8S1N9Z4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0840132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="117" mass="14398" checksum="53E66331E81F7F08" modified="2022-10-12" version="1">MQISEINITNRITKRNKEQKNQILRLKQFQQVFIKQTDQDLFNDPETGKRINNYMLIINAQEYLIIVXFMNQYIKNFYKLELIKNKRSKLNKIILERIQFNNLKYQIQMLIIQYNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAS1</accession>
    <name>A0A8S1NAS1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD-40 repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0700218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NAS1"/>
    <dbReference type="OMA" id="NTEDIWK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="261"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="344"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="386"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="428"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="470"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="512"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="554"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="596"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="638"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="680"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="722"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="764"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="806"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="923" mass="101120" checksum="593F1E71D735E8F6" modified="2022-10-12" version="1">MKGSFKQKEALAEIFAQVKNVEEQIFGVILEILRKQKVQDCIGFLSEIGNQSQLELQILLQGENLKQWDKEQKLSVIGNDIKQITDVLKKLQDHDFNYKDFSSEENEESTLSLVNSIKDNRRIIQFLQFLVHLTSIDETLIQGGSNSLHLLVQMKVDLRIQNFENIKIQNSSLMGANFVRCNLSGSQFNNINICGINLNGALLFNCKWKDLRIHELNKLNGHTNPVASVCFSPDGNTLASGSLDKSIRLWDVKTGQQKAKLDGHTHYVWSVCFSPDGNTLASCDDQTVFLWDSKARKQKVKLFDPNQRITQVSISPDGATLAFCSILNPICLWDVKTGQKKAQLDGNCGTVNSLCFSPDGNTLASGNDYHSIHLWEVITGQQKAKLDGHQGIVFSVCFSPDGYTLASGSSDKSIRLWDVKTGQQKAKLDGHSDFVKSVCISPDGNTLASGSIDKSVRLWDVKTGQQKAKLDGHTHYVFSVCFSPDGYTLASGSYDNSIRLWVVKTGQQKAESDGHSGIANSVCFSPDGNTLASGSSDKSIRLWDVKTGQQKAKLDGHTHYVLSVCFSPDGNTLASGSYDHSIRLWDVKTGQQKAKIDGHTSGILSVCFSPDGNTLASGSHYSSIRLWDVKTGYQKAKLDGHSGSANSVCFSPDGNTLASGNDDNSIRLWDVKTGQQKAKIDGHTNGILSVCFSPDGNTLASGTYDKFISLWDVKTGQQKAKLNGHTNGILSVCFSPDGNTLASGSYDNSIRLWDVKTGQQKAKLDGHSGSANSVCFSPDGNTLASGNDDNSIRLWDVKTGQQKAESDGHSGSANSVCFSPDGNTLAFGSSHKSIRLWDVKTGQEYKSYDKNYKDLLIQFKIPLQQNSPISEVSNYITTLFISQSTIYQAQGALILKGEFISHHGIDLRPFFKSKGSCILEDLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBR5</accession>
    <name>A0A8S1NBR5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0840007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBR5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="13051" checksum="EE760621D24BF4CA" modified="2022-10-12" version="1">MNQNKIFKLQLIHKQNNDCEQYFNQNYKQIVDLVENFDNNLKNLKKQIEQPIIEDHHTLSKVEEIENEDQELSQFQIQQEDKEELEQQLNQQQEQDQIIIQDQINNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ND29</accession>
    <name>A0A8S1ND29_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0830023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ND29"/>
    <dbReference type="OMA" id="NVYTEQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="253" mass="29837" checksum="BADF663BE2C2FCBB" modified="2022-10-12" version="1">MFQSYIINRKLKTEFSKVTQTVRITNELQRSYEHKNYSFEYPSTYDQLPKKLRCQSTYSKLCGFRSHKYQLFHDIISEFAEHKKQRLANVIIQNLKKSNLPTQGSARNIFSKNGPNLNHNYSQFMKKVKDFKISEQNVVTQTPYSIQENMTLTRQPSLEQVLETKQTITLSCSRKQSPKMLFKPQQFILKSKFKKNNALEKILTEARNQIKTSQSPRSVHISQHKKTKSQPINVYTEQNKLLQRVILKTKLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEC8</accession>
    <name>A0A8S1NEC8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytoplasmic tRNA 2-thiolation protein 1</fullName>
            <ecNumber evidence="6">2.7.7.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cytoplasmic tRNA adenylyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0860052</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEC8"/>
    <dbReference type="OMA" id="KPVRGIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="CTU1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000529">
        <property type="entry name" value="Cytoplasmic tRNA 2-thiolation protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03053">
        <property type="entry name" value="CTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056369">
        <property type="entry name" value="CTU1-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032442">
        <property type="entry name" value="CTU1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00269">
        <property type="entry name" value="TIGR00269 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16503">
        <property type="entry name" value="zn-ribbon_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="7">
        <location>
            <begin position="52"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Cytoplasmic tRNA 2-thiolation protein 1 C-terminal" evidence="8">
        <location>
            <begin position="286"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8090472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="322" mass="36602" checksum="D8ECAE149A887FA3" modified="2022-10-12" version="1">MVKYCQSCNVQKAFMVRPKTGNLICQNCFFQAFEDEIHHTIISNQMFKPGEKIAIAASGGKDSTVLVHVITLLNKKYNYGLNLYLLSIDEGIKGYRDDSLETVKQNQITYQLDLRILSYKELFNWSMDEVVAQIGLNNNCTYCGVFRRQALDRGAIQLGVDKIITGHNADDMAETFLMNLLRGDIFRLPKSTAIITGDDGDNGIATLPRCKPFKYTYEKEIVMYAHYKKLIYFSTECTYSPNAFRGHVRELIKNLEAIRPSAVIDLIHSCEQLDAPNQNQKMPQKQLCQKCNLLSSNKICKACTLLESLNQGRAKQILQIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJD1</accession>
    <name>A0A8S1NJD1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TmcB/TmcC TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0850194</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="EMHESTC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057352">
        <property type="entry name" value="TPR_TmcB/C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25474">
        <property type="entry name" value="TPR_TmcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="983"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1179"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1283"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1505"/>
            <end position="1531"/>
        </location>
    </feature>
    <feature type="domain" description="TmcB/TmcC TPR repeats" evidence="3">
        <location>
            <begin position="476"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="866"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="867"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="882"/>
            <end position="894"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8090291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1576" mass="185821" checksum="FCFA7D04924E2DCA" modified="2022-10-12" version="1">MIGQNNQSLQISIILSVFQFFQLLYFPFQIQIQYAWHNQKLSSKIQKFLQYFTILRMLINQSTSLYLMVMYSMISIILLTILLMIILTIQKRSNNQKVIILFLAKSIQFIMSIGFAEILRILLGYLLCKTDQNGNLRMLYLVDQECWVGDYYYHAIFVIISLLLFILVVLVCSQLFIELRNNKKNTFSQRQANSYSQIFVYIIFSMISYSLIQLPKYSICVIVIQNITSFIFFYRIYYKNPFYNQTVQKIWVSISGIVFHTNLMQLMTFLFNHVILQNTMLGWLITCPMIIIILQGRPSHQIDLIKVNLARYQSTEMIVKDCEYLVELSDLYEKDQSKEIQVLGFLEMHESTCLQSNCAIKINKNLSVKFKENPKYTDIRLILKELINQLYQNGLNKFPQSIDLKIHYSYFLYDHLQEGQKALSELEKAEQLNPDFGQQFQIYQMKKTLEDSFQKSSLEVNTIQYTISNDRNIQFQQNQLKQKIQECSYLMLQFWSQLLEEIPNLTTLQIIGRKVLGSIHQIRKQISIMQKANAFNQFSKKFLYKFSEYVFYIIDTVKFASMNINQQNDEVNFQTKFLDQNQQLGSQDLGLYSQPVIVLELFSNNNQISIVKNINQATCSLLGFSKTDIIGHPLAQLLQTNYSKLHQYVVQNYFASDSQDQLSILPQRQQFFQTKSQYVILTKSSGSIIQTEKGVFQFLRLTKDINYRNFAYIIFNEDGKIENISSSCISILKLDIRKLQLKQLNIQQLFPNLLSNKVIYLSKMSNIIYDFQKSKDKSMIFEDYEKEELNSNGFMGQLFEISEKNTQIKKGEKQQTFGYYLTVEQIETKQVIPSPILKRYQTTKEFTYSLEQRAFIYGIATSQYDSVRGDNEHQEESKQKIFIQSRQQSRKQNSLTQNRFQKAYGFDIKTLRLFKGEIKEVIDTNEEEEDQDYIRENTEHSNLEIIINKQQSTKINTQNELIQYLQQISYPAFLRRLVLLNNILILVSFTLSCIMYSTLYITFSKFQNAIELAAASNQRFSSLLKIQSNLQDLRGFNLNIEELEFQNIGENFINLNFLEIKNELNFLLESNKLLTSSDLTINKKYQTYIDQFEEPYIQMFSVDNSHQNFTYSQAVQSLIARAITLNNSHLSKFIDSNEDFHYYLHNSFNSIAKYQYVSQNYYFSNIYSLLDDLDTFENIFFILSSSCLCLIFIFCQLFVRSHQSHIRNIISAFLEIKEQYLKQIVNKIQNFIQLLQTNDEDDVDQFELEQNENQEEHELGELTKNSKKRKSQFSSESENKIKIQIIIVTLLFYSYFCYLYFSSITIISYTNLLIPLVNITSYIPTQYRLIDNSIKEMLYDANAIIMNQLNSVDKMNQMIDQIQNLDAELHILNQQNEHILSTEYIKLFNEIYILNPCAIIIENDLSVTNTSCLTFNDNILEDGLAVAISSFFENACKIIQEYYYYDPNTIYSNLTFNLSSNHKQNYTYNILNTKESNQNRKMQKVFIRICHLSLIKELLHQLSNYFSFLAFQMTLLFMIFCIITVFIYFLIWIPILTNFYFEAQKNLETLTIIPVEYFSKCVQIQEHMQLLKELNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJZ2</accession>
    <name>A0A8S1NJZ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0840108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJZ2"/>
    <dbReference type="OMA" id="CVWPGMR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF94">
        <property type="entry name" value="TENZING NORGAY, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="279" mass="32164" checksum="F91AEB6FCD5F6D37" modified="2022-10-12" version="1">MKLFRFAYSFSKYSSQNHSLLQQIYSYNYRDHYIAYLLLDHQSKSIISIDPGDFEAAFYNIKNLEVNSGYKLNYILQTHGRSNHTSSVLELYSKFKDAKVYSGIVENKLGTLFQTDFAREFQPFQIGDLAICFIQANGHTFDNYMIAVTDTNAQSTKIPILFSGDTILVNNVGEIEDYGAFFATLQKLRGFHDETLIFPGHLPQQEVFLFAKTLEPDNIIVQNKMGLDNLIPTSLIEERMGNPYFRYSQLNGKVKESDPIKYLKKLKNIQNKYFNGQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NMC8</accession>
    <name>A0A8S1NMC8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PSI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0790121</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CATRTCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035903532" description="PSI domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="2239"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="71"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="129"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="197"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="865"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="1299"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="1707"/>
            <end position="1752"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="2098"/>
            <end position="2145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8087764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2239" mass="238311" checksum="9F550B7CFD712C74" modified="2022-10-12" version="1">MKRTLLIIALISIATCQVVNKSEACTCTQLLTLADCGRNPGCSWNTTKLACEVPQSTGPVTVTKNYGKSLYCEGQAQTDCLKLNECAWIDNKCTFFTSCTPYEKASKDECQEISKRCITDGQICVEIDLCSTYLTSTSCYQSKTTYCVWDEPTKKCSDVTECGQLPIALTKDSECRLYLKKECTAKPGGGCVESGTNCEDQTSQEGCVTNKARSVTCYWNDQDNKCTEKKCEFAKKSINTHADCQIFSDKCTANEGGGCVDLKTCADGKISEGCKIDSAKKDCYWSTTEKKCKDKVCVNAPNTLTTNSDCQKQFLAKCITNGGGCVDDTSCSSSTVQEQCAVNRFNNRQCTWNVTCKDKTCENAGPENVGHDQCSTYSKLCTGKANNATGCQNRTCENAPTTITTNPGCEEYLPGKNCITKKDGGCVTNKNCSDILIRDACVKDINKKDCYWDSVAGNCLDKTCATLPTRLNSHPACNGEINTCTVSSSGTCVDLICENVVDKDNCVKDKDGADCIYYGSCFQKQCSAASQSLITHAECQGYLQTCTLANTKKGCMDIPLTCSALIKENCELKANGEKCGWTGSACVDIVCTTAPTKTDDDYTVELCENYKPNSNCVPNQSKKGCMELVAKCESRTIIEQCNVAGTATNGGKTCLWDTINSKCYEKTCANATKDGYNGKVVVTSDEVAHNDCQAWLKDKDNKPLCVVGTANNVCAPLPINCSGLGKNSCVVANVLKVPNSSPVEYLNCFWNVTTSKCVDGNVCANIDKTSHNDCTNASNKKCTVSADGSKCVDRQACTAYTQEIQCKKDKNDSPCSWTEATKKCSDTLCLSTDEDSKSFITHDNCTAKSNQCTLTNLPCGLKKTSCANYGNEKACLGGLLGDKKQCKWDSDAKTCGDVADGDFVCANVKGANLTGKYCNTLNVKCSVNAARTACIDAKDTCGAYTEAPSCKWSKNEGDCFYTGGACKAVSTLKCSELSGLTDAECRTLKSSCIFGASGKCKPDCAGRPNPQTYNTCQKFDSECSVKNDGSGCYYIDKNCSQLPTSTCTKGKDGKLCKVNGATCSDLVIIDDDANCGNVTSATNGKLTAKYCGEASGNKCTAKPDGSACAKIEANCAAYTDTTLPLCYFSTEGYCTIDSADTTKCAKVDAQACANISLGGAVELSDNLCSEVKATCIMDPVDKKKCKDYTVDPPTCGDFKGTFNFFNCFHFLNTCTVNSAGTACITAEATCDLYDASEKCGYATTDGPCVWDATVSPAACKPKSCDAITTTDVAVPSIDTCSAKQQSCTVRTGGCMKRVACTIYTTKDQCVENLSGGKCIWNTNTNPGKCFDKTCTNADSTYNDHAKCQGVGNCTVKVSEDLTTQAGCINLGACSDYKVADQCKINASKKDCEWSLAETPNKCVDKTCTSADPATNTDYEKCSAYITDGNCTLAAKKNEDGTYTEGGCMPLALCTEYNTVNQCKKSKNKDNAGNLLNCYWNDTTKKCGDASCLNADDTYTTHDACVTYGNTASLKCTVDQDDKGCVPVPETCEGMSKGQCVDVDAKSNKCIWLEVSGTGSCATRTCENAVSPATAADCSNHLFYCTMIDTGSKCKTKTCEDYNFTEDAACAALFNNSKKCTTNGKFCVNRGTCAQALSQKGCVTDSSLNACEWIVPTDTTKPAYCVLKTCNTAPKEFTTESQCLQYFTPKTGSTCNTQQGGGCVQRSTCSAAKAQAACNTDAAGFICAWDADTSTCRNQECKDISGTTHAACQNPTDKNFKGKCTAGKGGKCAVVQKCVLTTVEAACVQGTDGPCIWIPDSSNPDGTKGACFLYDSCRSLDWGKDSQCKWISDKCTTNGKQCIGITSCAKTNVDGGCVTGTDGECITTVQALGSLNKVCTKYASCNSALFTTHQECQDANPKCTTNGTSSCIELAACSTYVREACKKNKDGVQRNSNGQITSTGDCSWDETTNACRDQNCSDLKLNTTTHSGCSAQLITCTTDGTNCITKDSCVKYTTNSACINSVGTEGLCQWVEGTGGAAGSCRVKTCDDIPAGTTIAACSIISTCTTDGIKCITKGTCDKYTTKTGCNSRGTDGICVWTETTTGTTTTGKCSLMTNCATGSKDQNACQQASNRCKWTSATATAASSCVDHTCESYNAASGRCSYFPNWDSTKYNICRNVAGKCTAADPKTLLETECYTQSAYMYSWNSKTNACSQCGTTIVTPNNSTNGNNTDNNGSTTTDSGYVLGVAVLLGYLMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPP4</accession>
    <name>A0A8S1NPP4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0950056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPP4"/>
    <dbReference type="OMA" id="MRGPKYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="92" mass="11015" checksum="A0D40009796B8A80" modified="2022-10-12" version="1">MQGPKYQQQNIQELCRSKFDDDEDPEIDQDVRFDRFGNPINKQLKFHICFRDEILPSQQVFDIHVVENWKLNNLIEDKEESDACCKQLCQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NR24</accession>
    <name>A0A8S1NR24_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0880134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NR24"/>
    <dbReference type="OMA" id="QCNDMEL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013584">
        <property type="entry name" value="RAP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08373">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51286">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAP" evidence="1">
        <location>
            <begin position="262"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="328" mass="38611" checksum="3511672CAF2A8122" modified="2022-10-12" version="1">MQQLSKELNALISKAIQFNTKLDPFTTNELFLKWKSISTNEIDNINQFINIGIDIVKLQNRYSRSIKKSYTLLNEYIQKYSKDLENKTCGNYLSLARTTHLNDQLSLQFIYNQMKIDSDVEFSILKYCADLQCNDMELVSQLIDNLHNKIDNQLLNQYLYCLFVLFPHDQSSLLINLIKDKNILEITPSNFLLLSELYQNKNIPIPDSWKIGDQDSILQKNKQSHLQKEIQLFLNMVNLPYEQEKLIDDLYQIDFFLPQFNQILEVNGPQHYVYDIEQNNSKIFTGKHYYKQMCLSALGYDVRNLCFLDFYKARGVTAKLIYLKSVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NU44</accession>
    <name>A0A8S1NU44_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1020132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NU44"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="94" mass="10871" checksum="674DF6D1460A66A4" modified="2022-10-12" version="1">MAMDNSKFEQMSKNFPSQLKFRLNPVYYGARVGIQAYLGLLVGGSLLIYLNTFNGITFPYLYKKQKVEYQAVPDFYSKQVLLYQRTVPNTVTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NV53</accession>
    <name>A0A8S1NV53_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0870077</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8090994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NV53"/>
    <dbReference type="OMA" id="ICTQEIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362:SF309">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8090994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="508" mass="59758" checksum="D30E810B763E4B71" modified="2022-10-12" version="1">MGNEPSSTNGKTDFLTQDSICGPTLINQMQQNQWRIGLQHPHILQTLGGQPEGDQFRYYFEICPITLASLLSERYGTKQYFPEEDLQALLLGIVSALSFLQEKGISHGDICTQEIFFDSNSSSFKILDSNLINGRGYAIQEFISGKLKYLAPEIIIQPTQQMSEYQLHKNDIWSLGMVILEAGTLKSSDILYKNGLQHKLIQDRINEIGIIYGKQFADNLKIMLNFNPNERLDSVNLFNFLLEQQRIANESEQQIQQQQLLQQQQQLLMKQQQLQQQQHIYQQQQQQQYYYEQKISNQSPVNRQQQQQKNYQQNHQNFQQQQYLYNQTQQSSPYRNQIQIQPHICNQQQRSNYHQQRPSQDFMMIQHERTVSIEKMPSRIIKKIIHPDSKIGYQDNSNSASRTVQNQYWQQKHTPQKSQKQSSPLQRQQNSGYQSQFIQQQKYNVISNKENQFPNNTNDQFRWKQDIGQSKLVIQQYNQQKPSIQGEKSEEPIRRVIVQSQALRDNFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYD6</accession>
    <name>A0A8S1NYD6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1010077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYD6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="82" mass="9941" checksum="1BBDAB4D515274F6" modified="2022-10-12" version="1">MIFKIIYKTFDETEVRIVNIRVLSWFSRICDIVENQWLSLESPFLGIDNPAYSQNISSPRFEEQLSIYQRKYQNIREKILEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P201</accession>
    <name>A0A8S1P201_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1030038</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P201"/>
    <dbReference type="OMA" id="YCDHETE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017069">
        <property type="term" value="F:snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040031">
        <property type="term" value="P:snRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039772">
        <property type="entry name" value="Bin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010675">
        <property type="entry name" value="Bin3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024160">
        <property type="entry name" value="BIN3_SAM-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315:SF0">
        <property type="entry name" value="7SK SNRNA METHYLPHOSPHATE CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315">
        <property type="entry name" value="BICOID-INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06859">
        <property type="entry name" value="Bin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51515">
        <property type="entry name" value="BIN3_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Bin3-type SAM" evidence="7">
        <location>
            <begin position="102"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8097073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="367" mass="43882" checksum="5B55D7ECC327F1FC" modified="2022-10-12" version="1">MNEQFRKFLLNQEKSDVQQKKSDSAPQIRLKLQSKRKTNVDLGLETFVQKKVKQDEKEDKKQPNLMEKLQMNPIDLVKSTKQYSYGNYKKYYHLRLQQKWEDPRLTILDSIYFENKSILDIGCNDGTLTLLIALKHYPKLIRGIDIDYTLINKAIEQMVHLDDQQKKIQKQEFKPIIEDLPVSFHKYMEQPMSKAIEQQYIHQTIEDMNKQNEHNNNSILFNQIAKNNIFPHNVYFRVQNIIGNKKYDEKYDTILCLSITKWIHLNFGDIGIKRLFKTISNFLNVGGHLILEPQEWKSYKKKKYYSSEFKQNYKEIQLKPQDFSKVLEKEYNFKLIQQINPDDESAIKKSKSTFRRPILIFEKQNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHJ5</accession>
    <name>A0A8S1PHJ5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T1170150</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHJ5"/>
    <dbReference type="OMA" id="STMPKFE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="17">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="103"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="301"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="454"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="470"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8102143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="533" mass="61176" checksum="C4E03655565307A4" modified="2022-10-12" version="1">MDHQNNNTKQRDFAVLLNRNPQTRPQINSTPIQKVFIDPTQRIYEDIIVSEYLEEHKIMVESNQIQVPQPFIEWKDCQFPNQLNKRISLKAFQRPTPIQASVFPIIMSGHDLIGIAQTGSGKTIAYLLPGLVHIECQRKKGGPMMLILVPTRELAMQIQEHIIYFSQAYNLNSACIYGGADKRPQEMALARDPDIVVATPGRLIDFLDAQVTNLHNVTYLVLDEADRMLDMGFEQQVRKIDSYIREDRQTVFFSATWPKTVQNLACDLCHNEPINLYIGSQEVTINKNITQETICLYQNEKLEELLYILEELSNKDKVLIFVETKKDCEDLASYLSEHGFFCMSLHGDKTQQQRDYVMKEFKASKCKILCATDVASRGLDVRDISLVINYDFPNQIDNYIHRIGRTGRAGDKGRSITMITLDAMDPRIAKQLVDLLKDSEQVVNEDLYDFAYSKPYQKSKRPNQRKQQNDKPITQIKSNNSTNLNNQKPQQRQFNLKPSSPVKQSTSSMPPPPELKKAFSDAPVFGFFNSKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJ61</accession>
    <name>A0A8S1PJ61_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1180145</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJ61"/>
    <dbReference type="OMA" id="NFETMNW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="38"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="74"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="187"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="257"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8102508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="381" mass="45912" checksum="F021BE61C82811B8" modified="2022-10-12" version="1">MDKKVSKKLKNFETMNWLKNRFKNQVREKYILNEKELKEQIMMKAVFKSIDRDKSKFLDRSELYDMFKRYGINITKTKLKEFFKRIDQDEDDKLNWNDFKQALQNQEALQMFAELMRKVKESTEKQGKQEELNFIPLSFPNMIQYMNYCVLREELIQKINSNQLNNQQKIKQCKNLLSLEDICYNKVVELKDDNDEEQEEAAFIKTAKKSYDHQQVAILRRLQEKEQTEQRLNRLRKLSTSQYYRSETEKVNRSLRQKSCQLNSDKNQNTQSEFKQIDQLIFENPKDLPIIKNEIKQKVQIPMLMSERIYESDNPYKYNHKIKENKKDSSIVEIPKGQHFLTQNPISLRTSVRLSTRKQQNSPNRIRILSQTELPKHSNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJT8</accession>
    <name>A0A8S1PJT8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1200046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJT8"/>
    <dbReference type="OMA" id="VNSRCIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="109" mass="12600" checksum="C0F0F2A88B56E26E" modified="2022-10-12" version="1">MDQTYLRSKRKYIKCSLQTRKLLAELVLIQGLKIKNAAKRLQIKYATAKSIIIYYKQNVIKKQQNCKPAKRCQYASIKSAISYTIVSKLAGQDVNSRCIHFLNMEVSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKK6</accession>
    <name>A0A8S1PKK6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1220023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="223" mass="26278" checksum="44622B982CCEF1E3" modified="2022-10-12" version="1">MIQNNLGDFIPKNLQQIINLKWLGQYGNLNQQIGEWKALWENKPILAGGRYNDNGHKEGPWNELFCNYSEIDQLIESGIYQEGIRVGKWVTSKLLYICQTQYGKMSIGEGMYDDQGRKVGKWVDLHPQFSKDFLLVFEGDYIENVKNGYWSTKFKYFSENKYTIIGGGTYENGVKHGHWIDLSEYFNKQVQKLLXCLYRIICLSRYFLLQFRSYNLFRFLNKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQK8</accession>
    <name>A0A8S1PQK8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1270070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQK8"/>
    <dbReference type="OMA" id="YLSSCAW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016034">
        <property type="term" value="F:maleylacetoacetate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673">
        <property type="entry name" value="MALEYLACETOACETATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673:SF4">
        <property type="entry name" value="MALEYLACETOACETATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="92"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8105435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="24544" checksum="845A0E2EB29F77D8" modified="2022-10-12" version="1">MSTSRLAIRLYTYRGYSAPWRVSIMLKLKQLQFQPFFVNLPKAEQLSNMYSQINPLQIVPTLEINGQLLHESMAIAEYLEEEFPRNKLLPKSPLEKAIVRSMCETVNSGIHPYHASRFYKYVHNYVSDINRQEISEPFLDRGFTALNTLVEKHGGDYAFGNQVTIADAFLYPAFRGDDVSYLVNLDKYPALKKVVENLDQLPEFAYEHPDSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRI5</accession>
    <name>A0A8S1PRI5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1280030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRI5"/>
    <dbReference type="OMA" id="MWQSGIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="280" mass="31151" checksum="35363D281CDC667D" modified="2022-10-12" version="1">MSITVSSCQQISQSPLNLSTAKQLWSFPKSSRFGKLENPNNCAVAFYDLPDQKEKRAAGVGYSTKYDFTRNGPKTPAPDAYKIVGELDINKSKNRGFPFGVSRDKMWQSGIMGGLNKVTPGPGKYESQSTLSSTRYTMRPKNSQDIMILTKNVPGPGAYNSVQGIDSKGKYPISKFTNSCATLFNPPRSKRFDDKQYSNDVPGPGNYKLDNIGIQKVIFQSQFQQTGFYPISNLHSSKCRSFPHDIRKTYSVSSMQTPGPGQYRLPSDFGYYESKSVSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSG5</accession>
    <name>A0A8S1PSG5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1290089</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSG5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="79" mass="9618" checksum="4DF3F2C2583A52FE" modified="2022-10-12" version="1">MLIIIIIFTQILNIQINIAPFLKVKSKNFYFNINYFESLREREVNFQEFDFWLGNLMYDQEENNSSKQHMLSFNSMKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTB4</accession>
    <name>A0A8S1PTB4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1300033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTB4"/>
    <dbReference type="OMA" id="TAQNELW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="194"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="356"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8106262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="429" mass="52922" checksum="83E48B529567848A" modified="2022-10-12" version="1">MNAPHPEKMIEKFNQQMDVLLKHEELQKKQRKINEQKLNIIFDRNLQIETKDRKLQEQILSSRYQNEQLKIQKEKLKQQQELEKQKKDKKIAQNELWQNEQKQHEKEIQQRIFEIQNKLKYEEFKKKTDDLINQLDYNLRKVNIRYLEKQEQKERYVNDYMNQKQKTLNSSMQMNESHNQNVYSNYQMQMKRIQEIFQQKLNNYQNKINKIYKQKYEKMDQISQKVQQSQEHLELIKERSNQIYQNKIKKLKEKMDYFQDKINLIEKKRLSDLLHNSNKEFEHIQQINQIRAKSENIIRNKSLNLLNKQMLKEQTFKLVQHQNQLQLEQKLLRLNQKQLTQQSQIIKADQFKQSYIQQLEQKITQRDMRLQDQIQNKLEIQKRRLKVIEDMEKQKRDMLLKLESEKITNTSDLKYFHSTPRIQSNLFIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1G4</accession>
    <name>A0A8S1Q1G4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase/HSP90-like ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1410023</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="DCKLRIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="549"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="578"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="620"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="649"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="2">
        <location>
            <begin position="1029"/>
            <end position="1143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8109529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1360" mass="161025" checksum="9B44D2AA77F8343D" modified="2022-10-12" version="1">MNQDMLSDPSSSSKFSDLLHFILNSNSKSNKFQKILPRLSITESLNNSIRNARSRIQSLKHEPNGFITSRIDFIQNTGDYCYKALNQQTLSLKQQRGRIEDSIERSVQGSPSVHSYQTTKFKYPSMLESIRQRRNYNLQKYLDDNLKVFCDIIKKKDIEKLISFLNYPFQYSQEQNLLNTFCELLYFIAYFGNTILQLAKATFLFKDCLKLSELCRNHQLKIKILIQLFSIAKQLKQYDKAYKFILKALSYAWAENLDDYEIDCYDKLGMCYFYQGDILKANHFHSKWVKCEIEPRDSYYRITSKEFIQLYERSQPFCREFDDKISRYIHIPFTNLKTGQSFDCNSTLKYNNCESIPLLNEILNGYEYTEYFLEYHYVDQAQKQYLKKSSLPRRALEIMSKYDKNKDKYIFDHKIHENPIYKLSLQEKVNYRKTKFYSLDSVEQNIQKFISQQREPFKKSEKIYVKESQRKDLSPENVSLIIYYFNQLKMQEQEFLKNENKPFLLKQQQQITRTVLQSLIHEYHILIKGLTNQEYQDYCNVKGACGQTIPLILILPISLAIGTLQLVLLFFKNIREGPLIPAVIQFILLQTAQTLIMYMILKACEVIPKKIENPLLFGKITSYIFMCLSIAEGTLIIERISNFNQFSQSYFWAIFGLIIWQKIIQLFIYDCKLRIGIVIFLYIYYVFRLDCDLFNKDIIKVISYICLEIIFIIDAELKNYSQYQFENNMQFRNNKEDVQFMIPQVQMQTQQQILEIITNQFPIIIFDQTKELLHISGVGLKQLQMEDDQNKIDLISAEIKLRKMEVIQLFTTNQQQKDNLIQLKRMAKFGSTCGTNEKGELLHSLINGPALQQLRILRQPQDNQRIIHYNPDGVLEQLLNNLINDQQSEYKGISKFNLPKNQFKYFQFTLIKVPKGNVFQIFLILEDITQFYQDINLNQKKEEEDLPIICQEFRQMLFCQQNFQIPLKLLNIEMKNTIKYSKQQYLQEDIFKPDTLINQLQTKYQNQINTNIKLQFQNNLKLNTGEFKTDQLRLMQIMQILLENSINNTKEGFIKIIIEDDKRQNCIQISVEDTGKGINQEIIQEGFNINQIRSLNIVNYLTKIMGPLFYCKKITGQVANKGIRIKSLQDCGTTISFSIRNLSLDEFSTQFIIDEEVSQDEMDVDVDEDEIMERYIRNDDQIQYLQRFNKGLRSFIINRQQIQKITKPLDVNTNKKSTKLVGMMKLQELGRFKNIQQQNGQCQCNQKLIINSNQDFERVMKQYVQDNVSFIHEQDSFKHINLKIRDKQCLNHSCQVYKQIIINCYKSDFNYRELINRILGVNNNFKIIVLIYNSNGDIDMLGTYIMMPIQIDVLLQYLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5F1</accession>
    <name>A0A8S1Q5F1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1440096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5F1"/>
    <dbReference type="OMA" id="NDYMNTF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="4"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="134" mass="16299" checksum="59C5113019D5768E" modified="2022-10-12" version="1">MNDVLIIDERLKQLQEEVKMMEQQYGFSEEFQLKEVGNRLKKEDNSKQQTKKQTNRYQNLPHFVQQKEIKQQKMKAQLSNKWVEIFQEKPSIMQFYDVDRFLPTLQSPYDIQIKPTVPNDEDIYETYIDNLKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q879</accession>
    <name>A0A8S1Q879_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1470068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q879"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035728923" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12961" checksum="6560868272481A59" modified="2022-10-12" version="1">MKLIQIAILLSFLSILLSKRIHLNQEIQQCIREKCPEQLKLCYDDNACQLQLNKCRPKVDKPPKQGTKIKPFSNVLSCLKKNENSYNLIDCAYSNCEKTPQSQLYQYLLGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8B2</accession>
    <name>A0A8S1Q8B2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit TIM22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1490054</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the TIM22 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8B2"/>
    <dbReference type="OMA" id="FETNICQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8111819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="146" mass="16281" checksum="56D8F5D94F5B1058" modified="2022-10-12" version="1">MAQEPNPFKHVTFMDTNCAAKAGMVTVAGGAMGFLMALFMNAVEMRDMDYGRTKQSTRYVLRRDINKMFGMAKGFAIFGAFYSIFECQLEKLRIRDDATNSFLSCMFSSMVLAAESVGWKGLMMSGLGGGMFGGVMYYVQIKFMNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9K3</accession>
    <name>A0A8S1Q9K3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1420014</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1480099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000146">
        <property type="protein sequence ID" value="CAD8109845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9K3"/>
    <dbReference type="OMA" id="RIHATMY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="105" mass="12183" checksum="D8374E4B57508829" modified="2022-10-12" version="1">MATGGQILRYNGGTCYAMCQDVFSWYNPSIQICWKGCDYATGRVNDPVLRQEAEDMCKRYTAEAMWTKKGELDNIEDLRIHADMFPENPRNIYRACLAGVRRQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QB68</accession>
    <name>A0A8S1QB68_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CDC20/Fizzy WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1530062</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QB68"/>
    <dbReference type="OMA" id="IQLENHP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF1">
        <property type="entry name" value="FIZZY-RELATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="5">
        <location>
            <begin position="159"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="203"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="286"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="329"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="417"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8112902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="471" mass="53767" checksum="0DBC5A3B45D83B4A" modified="2022-10-12" version="1">MSKLKSQTVSYQALCNDASIQLENHPQTKQENQYNLSSTDYKRQKSFKGDRFVTQLKKNFSILPENKLESQDIASAQMALEMLYKQQILEQEPIFESDQMQFINQSGFQYNQQNNFNIHNSKIYNSILIDHKYFTISETLSNYYGKYVRKIQKVPFKVLDAPQLQDDFYLNLIDWSSQNTLSVALNSCVYLWNAQSSKVTKLLDLHNDSVTSVAWSLRGPHLAVGTKIGEVQIWDAIKLQRVRTYKGHIARVGTLCFSDNVLSSGSRDKLILQRDLRLKGNYFLKQSAHKQEVCGLKWSPDGQMLASGGNDNKLYLWSSHKQDKPIFRLSEHQAAVKAIAWSPHQHGLLASGGGTADKTIRFWNALEGKPLQKEDTGSQVCNLMFSKIENELISTHGYSQNQIILWKCNNMKRISTLVGHTCRVLYLAMSPDGSTIVTGAGDETLRFWNLYPQINQDFKQLNGPLHIPTIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBR9</accession>
    <name>A0A8S1QBR9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1540103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBR9"/>
    <dbReference type="OMA" id="KQQLYCM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="131" mass="15471" checksum="FFD04F33CC65A3A9" modified="2022-10-12" version="1">MTQLQQMDSRLVSEFQNDLKKSQSDMKNTQCHKILEEKGKKYMKIKLNTKQQLYCMVQKQGMKIKDAALILGIKYATAKTIIFHQRQKRKAKRKCGERMCGYTEKYGQRITRLQIINIIGNEVVHQQDYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDD8</accession>
    <name>A0A8S1QDD8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1560051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDD8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="33" mass="3873" checksum="777AAF1CB69D539F" modified="2022-10-12" version="1">MEIVQFAIWDNGLLTPGKMDNARNVIHQEIQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDI7</accession>
    <name>A0A8S1QDI7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD-40 repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1560105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDI7"/>
    <dbReference type="OMA" id="MATFMSP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="218"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="260"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="302"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="344"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="386"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="428"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="470"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="512"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="554"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="596"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="638"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="680"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="722"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="764"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="806"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="932" mass="102717" checksum="E5DA127A8CF835CB" modified="2022-10-12" version="1">MQSSFKQKETLADIFAKVKIVDEQMFGVILEILKKEKVQDCIGFLSDIGNQRQLESQILLQGEILKQRDKQKLSAIGNDINQIADILKKLKDHDFNYKDFSSEENEESKLSLINSIKDNRRIIQFLLFLVHLTSVDETLIQGGSNSLHLLVQMQVDLRIKNFENIKIQNCSLMGANFVKCNLSGSQFNNINISGINLNGALLFNCKWKDLRIHELNKLNGHTNGVVSVCFSPNGKTLVSGSLDKSIRVWDVKTGQQTAKLDGHSDYVRSVCFSPDGNTLASCGDDKTVYLWDSKARKKKVKIFGRNQIINQVCFTSDGTTFAFCSIDNSIRLWDVKTGQQKAKLDGNSGSVYSVCFSPDGNTLASGSGYYSIDLWDIITGQQKAKLDGHSGFVYSVCFSPDGNSLASGSCDKSIHLWDVKTGQQKAKLNGHCDTVRSVCFSPDGNTLASGSVDQSIRLWDVKTGQQKAKLDGHTHYVWSVCFSPDGNILASGSYDNSIRLWIVKTGQYKAQSDGHSGPANSVCFSPDGNTLASGSSDKSIHLWDVKTGQKKAKLEGHTHYVQQVCFSPDGNTLVSGSYDKSIRLWDVKTGQQKVKLDGHTHFVQSVYFSSDGNTLASGSDDNSIRLWDVKTGQQIAKLEGHRDTVRSVFISSDGNTLASGSLDNSIHLWDVKTGQLKVKLDGHTQGILSVFFSPDGNTLAYGTYDDSIRLWDVQKGQQKAKLNGHTKGILSVCFSPDGNTLASGSYDKSIRLWDVKTGQQKAKLDGHTHQVQSVCFSPDGNTLASGSYDNSIRLWDVKTGQYKAQSDGHSGPANSVCFSPDGNTLASGSSDKSIRLWDVKTGQEIKSYDKSYKDLLIQFIIPLQQNSPISEANINNFILLVSNYITILFISQSTIFQAQGALILKGEFINHQGIDLRNFFKSKGSCILEDFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKA6</accession>
    <name>A0A8S1QKA6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIPF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1620117</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8115117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKA6"/>
    <dbReference type="OMA" id="PEQKGCC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039765">
        <property type="entry name" value="Yip5/YIPF1/YIPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="7">
        <location>
            <begin position="51"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8115117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="25476" checksum="DAA77402ABDA9877" modified="2022-10-12" version="1">MDFFQEEEEEEHPEQKGCCAFLTIKYYQPYFNVSEDDVIDRVKASFLPFRKDFHERVAGNPDLWGPLWISATIIFLITAVANINQMNLEGQTTYSVAYVPQSAALLYCISFGTPVILTLVMKILGSDIRFFHTLCLYGYSMSILMPITILCVIRNSILQWCLVGYGMISSSSFLIMGMRKILGDLEQSKRYIIIGIVLAMQFSLYLLYKLVFFKVIEKNSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKF5</accession>
    <name>A0A8S1QKF5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1620006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8114895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKF5"/>
    <dbReference type="OMA" id="LFTIPGW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="358"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="397"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="434"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8114895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="479" mass="56395" checksum="137757D56798E7AB" modified="2022-10-12" version="1">MNPLNIQSENKESELKEEQAVQNEQEEVRTLTNLSIEDKIKQKKINDDHLERNKKRLIRQFTILFIITLFIIIERILYSIIVGVENELLSDFQSLFNLRKSKLTEFDEMDYFNDNFLFDMAGSVHRNGISIMIFTNYFITLYVGFDALIAVKILYTSYLSVFIVAFLQLIYSDPRPFWVYEKLVTSLCIPSYGNPSSFVCQLTFTLFYTVYCYKTKNHTRSFKTYDQKIDRKVKIVQLCISLFVFLYSILLFLMALQYLINMILGLIYCFVFYAFYVTFENQINNIIKHSTIMNIDSKRYVFYISFFLLITETIAAMLISNEYELVNIEWANNFMNCLYMTDINAMKIPAQLLMGPHFTFQKTSVIFALIGALFGVSHCFRHINSLEWYKGDKRKRILRIIVANLFTIPGWIFALNVESIALNSRMYEWGASFFLMESVAFLLFYFFMFAIIPLYVFKYLHLNASSSSSYVVVKLSEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QMT5</accession>
    <name>A0A8S1QMT5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1670014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000176">
        <property type="protein sequence ID" value="CAD8115905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QMT5"/>
    <dbReference type="OMA" id="TKEPHYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8115905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="51901" checksum="6CD03372AA4A5525" modified="2022-10-12" version="1">MIQENDKFKEYQKMIDKINELLIQFDEQIQQNLKVAMLVGNTANGKSTLFNFLSGFEFQITKKDNIKTKYLTLKYPDEKYASDMANGVKSVTKEPHYYFNKDNNYLLIDFPGFNDTEGAMQQFFVQVLFNRIVIKTPIRIIFVVKIPDGNLANRGVEFQDFISRCFAQHIQEFSQVTLILNQYMDNLDDQDLIDDVKNQLKDISKNVNENIAVVRKIREDSDLEMKFNNQKRQEIWNVIEKSNPIRFKPTQFKKQDELASLIHKKCDQIIQNLVSEICKQIDNFLNDLSKEDIEKLYQKFHDIKLLVNEQTDQQIFCRYQVLLKQMQEIAEILGWQNNQKIEIDTFLKIFKFVSKEEDIIEIKDFLEKNSQLLKNNLIIQKDTILKKIMEVEKRNLEDAERRRNLEDAETRRNLVDAERRRNSQNLSRKKQSSCQIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPA6</accession>
    <name>A0A8S1QPA6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T2050005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000214">
        <property type="protein sequence ID" value="CAD8117633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CDPTYSD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="55"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="89"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="123"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="191"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="225"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="259"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="293"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="327"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="361"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="395"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="429"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="463"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="497"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="531"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="565"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="599"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="633"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="667"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="701"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="735"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="769"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="803"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="837"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="871"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8117633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="918" mass="104040" checksum="828C34BCC4A4E595" modified="2022-10-12" version="1" fragment="single">MQYEQAIAQCEKVLKEEPQHLHALYRKSFSLQDLNQHSQAVSCIEKALSENFKYSIGYILQGYSLKSIGKQKDAIDCYDKAIQLDPYYAQAYTNKGASLDDLGQYNDAINCYNKAIQLDPNDAQAYFNKGNSLKNLKKYNDAINCYDKAIKLDVNNASAYINKGNSLKSLLKYSDAINCYDKAIQLDSNNAKVYNNKGASLIDLCKYNDAIDCYDKAIQLDPYYAKAYTNKGASLDALCKYNDAINCYDKVIQLDPNDAQAYFNKGNSLYNLKQYNDAIHCYDKVIQLDPNDASSYISKGNSLQNLKKYNDAINFYDKAIELDTNCSQAYNNKGVSLKSLKKYKDAINCYDKAIQLDPNYAQAYTNKGNSLSNLKKYKDAINCYDKAIQLDPNYTQAYNNKGNSLQSLKKYDDAIKWYDKAIQLDPNYASAYINKGNSLQNLKCYSDAINFYDKAIELDTNCSQAYNNKGVSLKNLRKYKDSINCYDKAIQLDPYYAQAYTNKGASLDDLGQYNDAINCYNKAIQLDPKNASVYINKGNSLQSLQKYSDAINCYDKAIQLDPNDASAYTNKGTSLDDLGEYNDAIVCYDKAIQLNPKNASAYINKGNSLQNLQCYSDAINFYSKAIQLDPHNSLAYFNQGNSLQNLKKYKDAINCYDKAIQLDPNYPQTYNNKGNSLQNLKKYDDAISCYDQAIQLDPNYAQAYNNKGASLQNQKIYNDAINCYDKIIKLDPNYASANFKKGASIDNLYSYNDAISCYDKAIQLDPYYTLAYINKGAYLDDLGKYNDAIKCYDKVIQLDPNYAQAYINKGNSLQNLKQYNDAINCYDKAIQLDSNQASAYINKGASLDDLCKYNDAIKCYDKVIQMNPNQALSYMNKGNFLQNLKQFNDAINCYDQAIQLDPYYTSVYIHKGYLLTQX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QU12</accession>
    <name>A0A8S1QU12_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2890002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000298">
        <property type="protein sequence ID" value="CAD8119121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QU12"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8119121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="198" mass="23942" checksum="3E37868CB212A102" modified="2022-10-12" version="1">MRMLNTKRQLLIACFIFKFPLHQLKLKYIWMLSKKYALSLVKLQWILMLSQVGSLQLQGFIEFFMLIHLVDSIQTFFDKIFYKSSEERYLYQKPYYFFNYEKIFQKQSKLINKYLDVRQILINAIQFTQKIEDLQDFSNKIDMLSSRQIKFISRHEVDELLVQKNYEELINSYGSINSEHKIGNQDYCVIPKLKVQIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QX43</accession>
    <name>A0A8S1QX43_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T2780003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000287">
        <property type="protein sequence ID" value="CAD8119010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="281"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="326"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="57"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="181"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8119010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="995" mass="114836" checksum="0B8FB55E46E97EF2" modified="2022-10-12" version="1">MIQSKMIEKEEELLCSLKHKLPIQMVACDNNLKRNQRLLCPECMENLASKAQLMSFKKALQNIQDNQKQKKESVENVIMISIKLIEQLQKNLFQLKSNVVQLLDQLIGNVDEWIKHVLMIGQQNVTYSFYDELDKLINDKKLDQFNPQTLIDQINQINISWNQKSEKKLNSFKSFQDSQKCEEILKKLKKLSGTKENIQEQILNQKQKEVIQVENKQKQLEQQLMMYEKVQFQLIDYSNYKSSYCLAMVFNKDGSIIITVDFYIIEIWNFEQGRFKLSNSYNEHSNAITCLVYSKKTNNFISGSDDHKIICWKQINQNEWKCSQPFEEHKNSVLCLILNKQEDQLISGGQDLKIIVWQVDFMKNHLTYLYSLDNHSINVVSLSFNQSETLLASCGFNEFIIWEQGLQGKWELKYKQNVENGCKIHFINDQQFIWVTQDKQINDILVFELLNGVFKQNSNKTITLINNNQCEDYILFPIIHNKDRNVILXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKLFELLNGVFKQNSNKTITLINNNQCEDYILFPIIHNKDRNVILIRHKYHIYLIRQLNDGTFQILAQLNCQTHESFGTMTNNAQYLVFWDNKLRKYSSYEILYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQ66</accession>
    <name>A0A8S1JQ66_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0080248</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQ66"/>
    <dbReference type="OMA" id="TPLWNMP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030391">
        <property type="entry name" value="MeTrfase_TrmA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045850">
        <property type="entry name" value="TRM2_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45904">
        <property type="entry name" value="TRNA (URACIL-5-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45904:SF2">
        <property type="entry name" value="TRNA (URACIL-5-)-METHYLTRANSFERASE HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01231">
        <property type="entry name" value="TRMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="492"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8044682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="63611" checksum="1A20F6419E62773B" modified="2022-10-12" version="1">MKPQICLLGIQKFAKDKQVLKQLQEQLGDKFINPTEIIKPKMKPFCFLQFIDEDEKNKFVKLIEDNPELKLRIGEVHNENAFEKQLEKKIKQQKQNEEEVKQFQEKHQNRDVIERKENELALQTAPLYQIKYEEQIEQKMVELKKVIKKLRNQFIQKNGKEYAPDWILDTNLIKINDPIICDEQFRFNYRNKSEFTISYNSQKEVCVGFNVGSIQKNNDSVEQPNTCPVTPAIAMEIANKFQTYLQSSKFQPFNRQNGSGVWRMLSVRKSDRTKQLLISVSINKDNLNEDEYVLICDELITLFKTTYGEFSLEGLLLMHSNKLGQDLASSADDTKILYGNSYYQEIILDKKINISYNAFSQNHTPQCDKLYQVIIDLCKNKKITKFLDLCAGSGTIGICVSDQIQDILAIEMNQSSCKDANENYKINNLQNCQIICSKVEDVIKKVAQDLKSFEGKQQLMACLDPPRAGVLNNVIEAIRTCKGIDHIIYVSCSPPQVIDNLIQLCLPQSKKNRAPGFKLVNVTLVDMFPQTSHFEAVFYLERDYGILSELM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JR82</accession>
    <name>A0A8S1JR82_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JR82"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="24234" checksum="4C36B97D44A79D2F" modified="2022-10-12" version="1">MIPKMVRKQLHHKLIEVLNILLSQSSHLFWLLFRQRYRLHLLKQKIFKFMIYISIKQALIYQSIQRLIYQYSWSLEILEIFDIRDIIKEIIKSQINQNYIYIYTLFDKEGVQLRQNDAIQKSQIRVVVVSKTWCFLFGIIIVMRCVVVYALLLMFNSCNQFQQLQKLRAYNMIVIALRLIIYKQLENERFFGVFLMRVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JT78</accession>
    <name>A0A8S1JT78_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0100070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JT78"/>
    <dbReference type="OMA" id="QFCFVQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF253">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="110"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="117"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="162"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="166"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="216"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="216"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8045874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="321" mass="38329" checksum="27112C68DD04C0D1" modified="2022-10-12" version="1">MSYDEDFNFDDLNNDQFCFVQENQFENDILDQNNQLQFECDQLRDIDCQNEINQISEDLDAIQKMSSTHNFDIENNSGLLHKNQCFNNENKVDDCTFESRSRSQSREIEKNTGKMKKWTQQENDLIANLYYQFNGDWQRIIERMPGRTLTQCKQYWQRKHKSEQSKKSKWTAEEDQIIKDNVNKEENNWAKIAGILKTKTGKQIRERYINKLRSDIIDVKQQPWSNLEDQKLLQLYKQFGSKWSQIARQFSGRSDLQIKNRTNKLLKTQSGTQPQNEQLKLQLENLESYLELDKTCEKSQNKPKFEFPQYNSYSVSSQFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYG5</accession>
    <name>A0A8S1JYG5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JYG5"/>
    <dbReference type="OMA" id="MTAKQKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="274" mass="31520" checksum="A0D9CDB307961631" modified="2022-10-12" version="1">MGNSNKVQQNVIHIEFMTAKQKIRKDKALIKNKVDQSNNEHLQDIICIQDLTQYYEVDFRQTILTSDETIHVKKIVDLVVNPNSKTPNNLIQISEFFKQNVKSFQIFFNNQMFSQDEQQYQFAYEPLYIEVIYKPKHWMEKAVEKFKTYSNKIMSALGFISKFAYIAIPLYTSLSALQPFLMPTLKTAKVALRSTQNPQTQTAQSVVKFLINTLKRASYIAAPLLSIVAQYMPMSKTMVAGIGLYLGKGMLWNVIKLCITIFCPFLAPLLMIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K212</accession>
    <name>A0A8S1K212_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K212"/>
    <dbReference type="OMA" id="MEQRLLY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="306" mass="36573" checksum="ADFCA41B8C959456" modified="2022-10-12" version="1">MKNKQNIVDTQILGLMRSSKINKQKFKEQKEYCAIKKEFQIIINIPKELGIREYLRTEIDIIFSTDYQINSNLGELCTNIFTVGYKVLQTDYFQDKQVFTLFPRNLQCVSIDVIYNLLEKMNLQKSKEDIQNFMLDHKKRNIQISQSNDVHYVDMYASQFPYESYLIRYDSTGSKVLKYINNVKYIQLMGITREMMEYNLMQTQILPCAIRVESYLDIWSKMFEAISRKSNFFTAELQNYNGQRTFVKMEQRLLYLVQDNDNSIYCYLYWIYHTNPDPAIAEENYQKELDSQNPKPKQCTYKQICN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2V8</accession>
    <name>A0A8S1K2V8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">proton-translocating NAD(P)(+) transhydrogenase</fullName>
            <ecNumber evidence="2">7.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0120286</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>NAD(+) + NADPH + H(+)(in) = NADH + NADP(+) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:47992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="7.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008750">
        <property type="term" value="F:proton-translocating NAD(P)+ transhydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05304">
        <property type="entry name" value="Rubrum_tdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000028">
        <property type="entry name" value="NAD(P) transhydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007698">
        <property type="entry name" value="AlaDH/PNT_NAD(H)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026255">
        <property type="entry name" value="NADP_transhyd_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024605">
        <property type="entry name" value="NADP_transhyd_a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034300">
        <property type="entry name" value="PNTB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00561">
        <property type="entry name" value="pntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006942">
        <property type="entry name" value="PRK09424.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10160">
        <property type="entry name" value="NAD(P) TRANSHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10160:SF19">
        <property type="entry name" value="PROTON-TRANSLOCATING NAD(P)(+) TRANSHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01262">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02233">
        <property type="entry name" value="PNTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12769">
        <property type="entry name" value="PNTB_4TM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01002">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="567"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="593"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="623"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="647"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="675"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="715"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="746"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="774"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="945"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1002"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1044"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1070"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1100"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1124"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1152"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1192"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1223"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal" evidence="16">
        <location>
            <begin position="36"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding" evidence="15">
        <location>
            <begin position="177"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="413"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8048023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1266" mass="134561" checksum="D3E1122C6F80787A" modified="2022-10-12" version="1" fragment="single">MLGFHRQATKQLLLSARTCFSSKGDKKPSHPKFIFGVPKETYPNENRVAATPESIKNLLKDGHQVLIESGAGLKANFADNVYQEVGAKVVDTNSVYDQSDVILKIRPPENTKALKESQTITSFIYPAQNKELLNQLQEKKITTFAMECVPRITRAQTYDALSSMANIAGYKAVLLAANEFGRFFAGQMTAAGKVPPAKVLVIGAGVAGLSAIVTAKNMGAIVRAFDTRLATKEQVKSCGAEFLEVKINGEVVDGAGIGGYAKEMGKEYLEAELALFLKQAKEVDIIITTALIPGRPAPKLLSKELVENMKQGSVIVDLAAETGGNCELCKPGELYNHQGKVKIIGYTDLPSRLPTQSSTLYANNIGKLMQYIVGTIKDPNGKMTQVDLNDDVVRQSLVTLNGELKWPPPPIVTSKSELQQADTSKAASHKKQEVQVDPYKQAWKNVINTSASLMAMMALGYQSPNMEFLKMLNTFALAGIVGYQVVWGVTPALHSPLMSVTNAVSGIIIVGGMELMDGKYLPGSFTGLLAAISVAIASLNISGGFLVTQRMLNMFRRPTDPAEYNYLMALAGATGVLGYYGGLYYGIPKEPLTNLSYLASSIACILALGGLAQQQTARIGNSFGCLGVGLGVAATLGYKGYSPELLAQWASMVMLGATIGGTVATRVAITDLPQLVACFHSFVGLAAVLTSIGNYLHVFPHLAEDPAALVHKLSIFFGIFTGGITFTGSLIAFAKLQNLLPSNPTVIPYHNQVNIALAALSVASLLIYNQTGSFAVGMTALLTATAAXGSIIVDLAAETGGNCELCKPGELYNHQGKVKIIGYTDLPSRLPTQSSTLYANNIGKLMQYIVGTIKDPNGKMTQVDLNDDVVRQSLVTLNGELKWPPPPIITSKSELQQADTSKAASHKKQEVKVDPYKQAWKNVINTSASLMAMMALGYQSPNMEFLKMLNTFALAGIVGYQVVWGVTPALHSPLMSVTNAVSGIIIVGGMELMDGKYLPGSFTGLLAAISVAIASLNISGGFLVTQRMLNMFRRPTDPAEYNYLMALAGATGVLGYYGGLYYGIPKEPLTNLSYLASSIACILALAGLAQQQTARIGNSFGCLGVGLGVAATLGYKGYSPELLAQWASMVMLGATIGGTVATRVAITDLPQLVACFHSFVGLAAVLTSIGNYLHVFPHLAEDPAALVHKLSIFFGIFTGGITFTGSLIAFAKLQNLLPSNPTVIPYHNQVNIALAALSVASLLIYNQTGSFAVGMTALLTATAAXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4H1</accession>
    <name>A0A8S1K4H1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4H1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="14357" checksum="D6E0B0B936EBB6C5" modified="2022-10-12" version="1">MKIHNTWFAQATYLGIIGAFFLLLQVLQFQNIDFIIELPKFNIFENFMISIQIITYFNYFQVSVSTFYIYSAYFINLEAQISFFYFSSTSTGIKRYSLKFMNFDLIVNQIKINLRKSIQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6H3</accession>
    <name>A0A8S1K6H3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vesicle transport v-SNARE N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0170020</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6H3"/>
    <dbReference type="OMA" id="MQNRIRN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007705">
        <property type="entry name" value="Vesicle_trsprt_v-SNARE_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF26">
        <property type="entry name" value="VESICLE TRANSPORT THROUGH INTERACTION WITH T-SNARES HOMOLOG 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05008">
        <property type="entry name" value="V-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Vesicle transport v-SNARE N-terminal" evidence="9">
        <location>
            <begin position="9"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="43"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8050387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="215" mass="25208" checksum="416498620CB43C65" modified="2022-10-12" version="1">MSQLSESSSELFVNCDEEVTAICKKLQQILTTIDIQEDKIFALKEAQSCIDSANENLKQMEVELQGFEKNIKDNLKQQFILQKRKLDEINKIYTQMKSKYEIQTSKELLFGKDQQRQKLLKEQEQIYDQNMKLQDAKMVIYGVEKEANEIQINLRRHTDKLSSNIEKQQPIRDALGNSYVLIKTMQNRIRNNKLVLFVVCGIILFAILIIIFMHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K888</accession>
    <name>A0A8S1K888_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0130371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K888"/>
    <dbReference type="OMA" id="MNSFLFY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="141" mass="16555" checksum="788C2D556636311D" modified="2022-10-12" version="1">MNSFLFYDPHFLLQQQLLYQQLVQYNSMLALRNTYSMSQSSTPQIQPKSPQECPTIDQNTIKNDETIDDQSSFELSKSNKIKKLIKKTKEAKSNKSNPSQNNEKKKIFLSMKDIKDVQYKKFKLVQEEQIQEGLTNLEQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9Q3</accession>
    <name>A0A8S1K9Q3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0180039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9Q3"/>
    <dbReference type="OMA" id="PREYDYT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8051323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="90" mass="10318" checksum="EBE92F70BB3CB55C" modified="2022-10-12" version="1">MGVCVSQEKKNKSFKCATQSQLNLQNFMITQDKQTTTLYHSTKTQAQVQPREYDYTNPHRQYNVLLNGVVFAVVNSVERSFSDEPINNEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KES5</accession>
    <name>A0A8S1KES5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210297</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KES5"/>
    <dbReference type="OMA" id="CVQHNND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="185" mass="22068" checksum="8E888D5BCC974D84" modified="2022-10-12" version="1">MKNPWTQQTVAQQQITYGICYNILNKKYQLQSDEKQQFDYITILNNANKIQEMKQKLAIQIIDQQFGDMIDPKKFEQRIQQLDSLEQIINNVLLCLQKCQICVQHNNDTKSFIRNDGSVYINIPFQYKTQFIQLLRQAQEKQLELVDPDQQIIQQQRCLKDLNEQQQSIEEIYSQIIQLIEQLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KF16</accession>
    <name>A0A8S1KF16_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KF16"/>
    <dbReference type="OMA" id="KEKNNRC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="183" mass="21530" checksum="B79EC932A42FD3E8" modified="2022-10-12" version="1">MNEQLNKMEKEKNNRCRLLHKVYAQKQKPPFNNMSFQIRVQNMLKQIQYAQNERLRCQSEFENKFPSILKLNPLSRPQNIAPLNLRLNTIRTSSRQSNLIIDGTTKKSQEKRFQQASQDSNLRLSSSPLRFDIKNKTSIQTLVLKDMIAANKQQIKLDQQTQVEYNLIENISSWSRKSSESIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KFV7</accession>
    <name>A0A8S1KFV7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210326</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KFV7"/>
    <dbReference type="OMA" id="QYIKWKE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01248">
        <property type="entry name" value="PH_PLC_ELMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="118"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="512" mass="60365" checksum="4ABA53375ECE0C09" modified="2022-10-12" version="1">MLNSKKLIKSNQLTELNNFLLQIKQAFVTDDFQQAIETANINDNKIPLQEIIENNIPQTKYDQQKQTIQQPQEDIQNLLQGLTTLLEKNLQESHNLYQIFQSNKQFNSKAENSNSSLKYQKEENVEDLQEDIKQNEIRLLQMQNSRLLSKIESLNEQITQLEQDIHENSNDQRQLISLINSTYCIQRIQYTVKQNTNTILGKINDEEIEQGVFVIVDDQIILSPSYLDENKMILNISQIVDVTYQNDLQIIIHYKEGKLIIILENQQEFSKIDLSLWSILRKNISINQDKLFENSLFERPFRQEINIDYKKLKEQLSHIIPLIDQEQKQQQQQFTLNIKNSDHFTEVHQSNNVQIQSHQSPELRGTLPKQMVQINQQTKLQHALLQLKQGFSMIKYSEGQRKPNIKIIYLTENEQYIKWKEELQPFDQKNTSEKKSIQISQIKEIKDNAQGSGFEKFKKYRKGKESLGITIIAKRNLELDAPKQEIKDLFLECLKILLENKQNNKQEQKNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KGT1</accession>
    <name>A0A8S1KGT1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KGT1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="194" mass="23494" checksum="128AF747076EC4B2" modified="2022-10-12" version="1">MSISNISQQQQLRKNKKINQRLEKIGDESLNFKPKSPYNYNYKFKKVRQLYTNTLTPLNERNIQNGAQNQQLINKIFQYKEVESQSISINQTNQNKQPSLYQINHFLIKISIFQGISKKNKSNNKLNFKKKNQIYQKMIFSYYQIELIRINIIRRWIFLVEKLRKYYIQCLRIVIHFIGQSQKCQSEISNYERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIE9</accession>
    <name>A0A8S1KIE9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KIE9"/>
    <dbReference type="OMA" id="QKFHKIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="308" mass="36355" checksum="EA5DD03AF56FA6E2" modified="2022-10-12" version="1">MLDVISTFSIHSCQSRRNRQTGNLSKGSTARQSTRPTQKFHKIQLDDLAFKTSPKSEQREIKGIQATNEQNRYKENENENKFESNIYPLIIFQKISYRQLLKEIKFEKDIKSLHPSDKRNLVIYRIMCELFQELQFEIQNILICQHPISKLFYIYFSDTNYCPISKCCNQNVVIRIGEALGGEIKCLNPKCPLGILTTFEIEEKILWNSKLQQNRAISTFISSGKYRTKSLLQSVQVPENNTAYAPKQYKQNQKSCKRSSTFYQRANQTNYEQLYKYELSKNLLLNSQIFNKNFDDQIDEKYRYQCKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJ59</accession>
    <name>A0A8S1KJ59_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0230033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJ59"/>
    <dbReference type="OMA" id="IMDCMKC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="103" mass="12569" checksum="56E87B2B6A78DCCA" modified="2022-10-12" version="1">MSENYNFSDFVKFRKGSTFGIGKIENFSREEDWFEYFEYILSEELPTGWFIYCILYCIQILHNTHFPIILIIVFFFFINFYQVSNHIMDCMKCLKQINQRKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKZ5</accession>
    <name>A0A8S1KKZ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0220113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKZ5"/>
    <dbReference type="OMA" id="MTWLTYS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8054605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="134" mass="15645" checksum="0C43E1D3E0CF0F7D" modified="2022-10-12" version="1">MSEEQAVDFTFSSLLPTAEKLMTWLTYSTLFGLISFPLIALTIDKQFAFHFWILTFLDAGLLFTLLYLKGQLSQIEREKKEFTQDESKKSQKKKKPLENEKNKVAQKAKKESQKHNSMNYNKAVGKQKSFQQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLC8</accession>
    <name>A0A8S1KLC8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8055023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLC8"/>
    <dbReference type="OMA" id="MHGKGQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="169" mass="19818" checksum="38ED1F94BBDC74C2" modified="2022-10-12" version="1">MSTSYGLHGEHVRIYRDEGWYEGGWVYNQMHGMGLLKWSEGHEYYGNWKNDERAGKGKFKWANGDEYDGDWKKNEMHGKGQYKWADGRKYDGDWVCHQMHGKGQYIDSNGNEYKGDFQNNNKHGKGKMIYADGSVYYGDWLDDQRHGFGEYESANGIQYSGGWFFDEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KMB1</accession>
    <name>A0A8S1KMB1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KMB1"/>
    <dbReference type="OMA" id="MISKLIW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33802">
        <property type="entry name" value="SI:CH211-161H7.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33802:SF1">
        <property type="entry name" value="XK-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="255" mass="28926" checksum="4FFA68BFFE42FE19" modified="2022-10-12" version="1">MDFVLNLIHQSQTIPFRQGVKFNNYNLVCIISVCAYILALLINGTIGVKTAKSTSDSYKLVVTPPSWAFSIWGVIFSTTLVALLWSIHSVSWSLETHLYFWLYCFTISVWILLWSIVSKITILLCKIVLILLTINIYLLWKSTLVLENDDWSIYLMRGAIAFQLGWASSAQCLNFCICLVHVFGATQDMISKLIWVCIVIAHSIYLGLAYCHSKQYNKNMLLNFGAYLLSMSWAIMGVAISYNKYSSGKAINKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KND9</accession>
    <name>A0A8S1KND9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0250096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KND9"/>
    <dbReference type="OMA" id="RRKAEYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="322" mass="38456" checksum="CC2ED18DCAECB497" modified="2022-10-12" version="1">MNQQSFVQVNQEHDQIKKRVNSLKFQLKDIDQKNFILQNKLKDLKQLSPNARKSPIAQLQEQLGLDRKQIREQFKPIVEKEGIYVRNERLKTESQLQNQRNIEIQQKKKRVKQIEDQDSEVLRRKAEYYEKKIIEVRQRQLQEMEMNKLIVNKKIFDINNLKKQEQQLLGEISLAKQKEQQLNNRFCNVVLSTDNSIMLPTIQKTNSRMSQKSNYKQRLVLGSHSVQRSDKFLDRLQRSDTKNNDNSFIDYDENRRLRIEQRKSSQSIDQLNIGCQTSFIMTDDKKQESVQYSENFDSYCSNYQEESSDNQQKNKSKQKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQF8</accession>
    <name>A0A8S1KQF8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0260054</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQF8"/>
    <dbReference type="OMA" id="IMQQSTM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000194">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000195">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342:SF14">
        <property type="entry name" value="DEATH-ASSOCIATED PROTEIN KINASE DAPK-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="7">
        <location>
            <begin position="665"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="7">
        <location>
            <begin position="767"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="448"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="448"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="462"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="487"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="507"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8057458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="859" mass="101070" checksum="5D4FECEE7B4904EE" modified="2022-10-12" version="1">MNIRQLDNIVSWLLSKFKSSPYRWNEQQIHSFCESVGLQWAVSIITIQTAPKQRSYNNQETHTYLNKLQIPLLDACTRQYEEILELCNAQSIYKLISHSIDINLYYQIQKLKIWSLIVLKYQHYREIFKKHYGSKFFSSSKCKSCTAEISCACQILSNLLIEIEIYIKSIYELSNIQNQNRRSSMYISSDIINLLLKSISHLPSYDQQPLFWDMDDIKVLLHVIKFQDYYQLLFQNQIDGFVLLILVNPPFKGDNLFLKCLLMSQQNYSQKSQNLLFQNRQSMVRNTMQTASKYHQINQFLHLLHYLLQILSLAAYKNQESYTEYKHLGQRKVSQIRWISLYYFNKDTHVGQANRRSTLTEQFKRSSKSYGSVESQMKNRNKTETLDQLEIKIMVTDQSLEQIKKQFSQPNVTHSAQISPNVQRDTNWIKFKNQTVNQFNIEDSVNKNSSFLIPDNDSNTQEQERDRQNTETHVQIDEEILSGSNVNQSQEEQSQQLSNQEEDQQDLNDQMQQQQQQQQQQDMGESVILGQATIMSQSTILQQSVIIQQPTIMQQSVMMQQSQIMQQSQIMQQSQILQQSQIMQQSTIMQQSTMLEKSIQIQQIIEQQVNQRIQQQRQIKEQILKRIEQITLQPLFDLFQLQQQTHLKLKCDEQQKLKVINIQGASFGRNEDCTFAFTDHSKISSKHCQIYYKDKAFYLVDVGSKGGTFIKINHNFVIEKDMSVYIGNRFGFKIIDINTETGFLEVRYEHEGTAKHKQIQLKRGDKFLIGRERSKNNFTFMHSQCNRLMSAKHMEIYYDYNFKAGSVKLMINDLESKNGTWLRLSEKQIVSAPFHLQKGVKFNLSFELMFDVFELVCDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQG0</accession>
    <name>A0A8S1KQG0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0260180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQG0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14738" checksum="B17CD03DA8098EA7" modified="2022-10-12" version="1">MPPKKKKSKKSKKSKKKEEYLPSIFNIPQYENPDIVTPKVDLIIKLVHPVTDLFTLKIRVPITTRVEQIHEEISKMHQGAISGIQVCRDRFSPEDVLDPKKTLEQCGIFDGEIKLLYDYKPIVGPLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTA1</accession>
    <name>A0A8S1KTA1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0240028</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTA1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="413"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="686"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="734"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="802"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="926"/>
            <end position="960"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8055926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1076" mass="124637" checksum="BB45967117D7969F" modified="2022-10-12" version="1">MTNSEWKLIYSDFYQQLITTHQSWKSLETCLINHIFLPQTRQCLTNNLEFIRLNDERKGIEKQFYYPHYQLRIITDVIYIRSQSGVNSQFQIQINDSSGPYVIFSRDYQQSTLQSYYKVCNFDCTLSCNGLDERRIEIKTHIIDTIVHQFPIFTISYCFQPQNDFMRIGLRNILIYVNSCHISCASCDGEAQFNCLSCYQGSPLGGVCLCDESNKYIHRLTGCIQECPRDYYLTDNSNYCQFNPRIKSMYNYFTSSTISNGNQIPYEPWEYYPDPFHFSNTNNIFFCSGQDQVGKFMYSQFMQLKLPKNEGLVFLRIRVSFHFFGWQPDSVLKLIVDQQSQMRIEKTLNNYTIFNGRLNKFESLSCSSENYDYLRIEAILKTYTTTPIIKFQAIQKLDSEFWSFNNVTIDYGLCQSNCTKCETFQKCLICESGFKLYRGTCVESCPSYSQINNQDCIDYEDIIDNSRYLIKAFYDMNTTFEEVTKVVDNFTDLNDNIQTSFTQAYYSFVPEKSVLGGVLVWSDGKFKKTFQNLQPHYQLSIRMNITYGDENNGWFKYQLDSYQSNKINNPDTGNINIAGNNKKETTIYQEYQYTHNSNQLDIVLSADTQGMSLEDAFMYVSEYFVIVHYCAPFCSSCTGPKITDCNSGQYSGYNLTHYCSSSEYLKYNSDTQIYTCEECNQLGCLECQNELICTRCEFSDNNLQFLLDQGECICYPSQYLSNNQCLKCSKYCESCFGSNNDQCYTCNSDFHRSINEYKCQCLNGFYDDGYNIQCIPICGDQMIVDGEDCDDGNNNPFDGCDQCKYQCQDVCLNCHNGKCYQCKDGYTYDQTTYKCLTTCGDQIIQGDEQCDDGNNDVQDGCYLCKLQCDINCINCQYGNCLKCDEFNGWYLNNSHKCQYICGDGIVSKQYEQCDDNNKNPFDLCDNCYLTCSDHCISCENGLCLKCDVGFRFLPQTKQCLPICGDSMIVGYEQCEDGHTEIINSCNQCKFKCNSKCLICQYNKCLKCEFGYYLNNNMCEQYCGDGIKVGDEECENIVQIVDKIVEVDVQFALKEYVNFVNQDIQKNNINVNLFVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTF0</accession>
    <name>A0A8S1KTF0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0250303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTF0"/>
    <dbReference type="OMA" id="VQYVSMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="124"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="453" mass="53455" checksum="D19CDBF7133A547B" modified="2022-10-12" version="1">MNQQRRLSPAPSSPIQRNAIISQHSNRFQFLQVTPSTHPIQYVQMQNYNEARQISRERDNNQIPFQQQQVCPKGFQSLRIMSQPDGNSSKSPVVPVMQSQPNIRPNTNQNSLQMYIDNALKTQREEMLKLLEQQNKQIDYMNQQQLLIIQQQQQQQEKKKESINIESQLNQVKEQLHIQITQACREMIHKLQKQQQTDFKALQVQIEKLKSQLYQINLSTSKQSEKLQQSFKGKENETEEYQYKKHIPQRNSLDNYDQYIKKYEQFNKQVNDYLINRKIPDHLSSILNTSKDSNSEIHDIEYIQSDHPLTDITNIQNTKQNKQYQSQPLQQIAQPSITQQFMTQKTTIQSNNQINEDSPIKALNEQMMIDSLDNINVHDPNNLQITKEEFDQLKSQRMSTIHENEDEDEELMYQVDENGFILSQDGHPLIDETGKRIQLTKQELQFYKSKLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVG0</accession>
    <name>A0A8S1KVG0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0290011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVG0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8059480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="25800" checksum="ECA39980409D39B7" modified="2022-10-12" version="1">MMYFPSLMVKQRIYINGTIQIKNLTGTKMYYHVARDKQSKNYLKGYISGLQLSSYCTTPINTLSPNCHYSSLTCYGPNSINCLSCQLNSQRIQSITQKICACKKKYVDIENELICKPLSEIFPQMKEVEQELNCDTLGNDVFTIEKKLMFICPGYSSFNTKKQDLIFKQFVLTRQLLKILSVHKTQSYMNIMKIFHIKLSTEWREIQNFKYNLKWKWRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0C9</accession>
    <name>A0A8S1L0C9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8060847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L0C9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="56" mass="6639" checksum="B7EAD68DA1195220" modified="2022-10-12" version="1">MMKKFQFVDCATRPIFNNVAVGYNYLLGAFSQYYQSLFYLCSNFIPQIAKIWINEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1A9</accession>
    <name>A0A8S1L1A9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0290271</name>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0290272</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1A9"/>
    <dbReference type="OMA" id="TQIMHIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000003">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25427">
        <property type="entry name" value="zf-CCCH_UNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="186"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8059944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8059945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="225" mass="26171" checksum="E15E6E794996024C" modified="2022-10-12" version="1">MYSQGPQYPNSKFKTQLCRHFTQNGVCALAIRCQFAHGPQELRQNAQQLQSFPEQTIQTNAYNKVQGINPMIVNYKTQLCKHFNPQTGQCKNGPTCTFAHGENELNTMPHLQNQYLLMQQQMKQMNQQQMQAELTQQILVMILTNMEHIFPGQQQIIALLKQGQEKAKSGDTQGASEIIKLIIHDQDRSKEEKQQYQQIYNNAQRHYDQKLKDIQSSQQQQQQLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L463</accession>
    <name>A0A8S1L463_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0330051</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L463"/>
    <dbReference type="OMA" id="HKYQTIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000019">
        <property type="entry name" value="probable aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044638">
        <property type="entry name" value="ALDH7A1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521:SF7">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 12A1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="4">
        <location>
            <begin position="40"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8062267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="534" mass="59816" checksum="1ED179CC4438E50A" modified="2022-10-12" version="1">MQKFRNIASFSTIPSYFSLDPNQLSARNPHKAQNFLRGKWIDSHKYQTIVDPMNGEPFITVPDVQDKQELLKYIESAKECPISGLHNPLKNPQRYQLYGEVCHKIAAELKKKEVHDFLVKLIQRVVPKHFAQASGEVVVTERFFQNFSGDQPRFLTRSFNVAGDRPGQQSTGYRWPYGSCAVVAPFNFPLEIPSLQIFGALITGNKVFFKGDSRVNVVMEQMLRLALHCGLPATDVDMLFGNGEATEFILRQGNFRNTQFTGSSKVAEHLTKVLNGKIRIEDAGFDWKILGPDIPDIDTQNYVAWQSDQDAYGSTGQKCSAQSILFVHENWVKAGFLDKIKQLASTRNLKDLSNGPIITWNNTQIKQHIENILKIPQSKILFGGQELKNHSIPSIYGAFEPTALYVPLDQIQKNKSVTTELFGPFQIVTTYSSDQINQVLDIINNLENHLTAGIVSNDVNFLRYVQSNTTNGVTYSGIRARTTGAPQNHWFGPCGDPRGAGIGTPEAILTVWTCHREIIHDTDVAPSGFKGQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5H0</accession>
    <name>A0A8S1L5H0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">intramembrane prenyl-peptidase Rce1</fullName>
            <ecNumber evidence="10">3.4.26.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0290303</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic sidechains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.26.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase U48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.26.1" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5H0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039731">
        <property type="entry name" value="Rce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046:SF0">
        <property type="entry name" value="CAAX PRENYL PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046">
        <property type="entry name" value="PROTEASE U48 CAAX PRENYL PROTEASE RCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="12">
        <location>
            <begin position="106"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8059996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="260" mass="30877" checksum="6B5697F01A70CFF3" modified="2022-10-12" version="1">MFISSVYAWKQIKRYFKMRSNAKLKTLSVAISVLLMILNQRFLIGNCSSIMECLYINVNLNSILYPLICNTFLFLGPIYNSILEKYLKNEGFVNKFNENPFKDDRMLIRIVLAPLLEEITFRALFYQNAENQYQYRLLSSILFSLAHSHKFFSFFKKERKFRSLYNYKETNKIQNFKTCFTKALLTTLFILMFTFIFGFYAASVFLKTRSLISVILLHSYCNYLGFPKLKQIFSNTKVNIIKILVVYFVGLVFFYYFITM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LAE1</accession>
    <name>A0A8S1LAE1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0320129</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LAE1"/>
    <dbReference type="OMA" id="NELNYCD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF3">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="486"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="647"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="500"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8061766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="688" mass="81666" checksum="611F05E9912851A1" modified="2022-10-12" version="1">MKGQYYSLKRKINTSSQLLRSNQLLSLAETNDQIQKNKTDSQLSNSHKKISRSINNLPKKDPNLDLAFMTITKPDSRYDFSKFPNLEKKTDVQRVNWFTKQNKQIEQQQNFLEFLNATRTQSTKNQTKTKVNVQQNKIDDKNELNYCDKYFVIKTPIKPILIKQKNTKLISMSSTMSRIKKKALNRNMNLEQFIEKNNELRRHCQTQQEHQYTQNSILEQLQIKNIQHINLYSSVKRGIKNNILHNQYKSEIKDLIELDDELTIQDYETIKELQKKSIFQNPFSNPHHNEINQFNSQSIDNNTKINEQNQNEQLLIIQKRKNKIKRVLLQITLFLHWMIEHKLKYEDIITGNVFQTKPYQSEKSKLCFQKVKANDIDLVNQFINHNKYLVYDYDNFKLTMLHHAVIRDYPEMAILILQNHAEINSKDIQGRTPLFYAIKGKCNQCVLILLYYKASPWGNVKNTYDKYLEFLDPKVRDLYKKAKTRTLLVIIKINIYIQMIILILFLISFSYAHDWDENTKVKLLNEEQYDLLNKDEGIFIMFYAPWCPHCIKLIPTWEIQGQQSNTAAVNCEQHSQLCSRFKIKGFPSLIYIPPQSKFGYKFYGNRTNDEFDLFIKGGWQKEGILQIEILSEYTLVDQIIDYLKDPMFIGVIVVMLFLIFMICCMNRLDSEREEIQKNKQQKVQKKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LD74</accession>
    <name>A0A8S1LD74_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0360064</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LD74"/>
    <dbReference type="OMA" id="QCKYLIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17744">
        <property type="entry name" value="BRCT_MDC1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051579">
        <property type="entry name" value="DDR_Transcriptional_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23196">
        <property type="entry name" value="PAX TRANSCRIPTION ACTIVATION DOMAIN INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23196:SF1">
        <property type="entry name" value="PAX-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16770">
        <property type="entry name" value="RTT107_BRCT_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="5">
        <location>
            <begin position="501"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="349"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="349"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="371"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8064315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="660" mass="75807" checksum="73DA056F4776119A" modified="2022-10-12" version="1">MIQVLDQNDREIYKYRLLQGENRFGSDPKQCKYLIRNVQGVLFSVALLNNDVYFTHFSNSDQVYKEATLKSDQKVEIPKNFIIEMVPLRNYYFQNFKFKLLNSIETIQQNNQCTLQTTQILEDFSGTMALDDILDIPQQFIPKVSITQQMSTTQVLSSQDPIVTSTLILDEIDQSTLSSLNELISQYSGINSMVKSIHSTTNDAQTQTEIIEVEPQLQKTRTLFSDLFKSKRQTSQISQQIDQHHQQPSLIEQTIIDSQMQNIDQNQQNNQQFLNKKADFKGTMIDDDLNKAFSSQQIKSVDKLEQSQIKRFNSIQSDNVQITQSKQDNNNSKIELNDLFGSLPELPNQQIQQQQTIKKFPQPKSNILNTRYDDSQEDKNKSQDQQEQTPIKVQNQNQKQTSSIWKQGKKQSKDDLKKVALLKKITDEISDETSEESNQSKQKLRHTNSIELPATKIFQQNDLKINKKLGNKKTLQKKVKSVHLIAFSGFQANEIPQANDLKKLGLEIVNNQLEKFDLLILNNPPKRTFKFLSALMLGAEVVTMDWLTNSIKQGKAIQKYHDYIPYSDEFINSFGLSIEQILQSRDTYFESNKNLVKIFDGKYHVKVDVTPSKAEIEYLIKLGGGEIQQKSGRNVITISDTKVGNSMPSDFILDGCMFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDQ7</accession>
    <name>A0A8S1LDQ7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Opine dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8064999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDQ7"/>
    <dbReference type="OMA" id="KTYNWAR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051729">
        <property type="entry name" value="Opine/Lysopine_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003421">
        <property type="entry name" value="Opine_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38015">
        <property type="entry name" value="BLR6086 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38015:SF1">
        <property type="entry name" value="OPINE DEHYDROGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02317">
        <property type="entry name" value="Octopine_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Opine dehydrogenase" evidence="1">
        <location>
            <begin position="189"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="403" mass="45919" checksum="FB13CE4838E41888" modified="2022-10-12" version="1">MIPTSIVVCGGGNGSHATVASAGRFENIQVNVFTRKPQDWKQEIVGLTKGSPWESLGNFVGKINKVSNNPQDFVGAKLWIIGGPAHVHHEILQKIAPFVTKDSFVGTLYAQGGFEWMCRSVFGDRIRTDNITYFGLYNIPWLCKIFKYGESVRIIGPKTKLQCALSNLNRKEELFLLLENMFQIPVLQAPNFLTLTLTPSNQIIHPARVYAVFRNWDGKQVFQPSEVPTFYEDFDDFSAYMLQILDDEIQAIKREILKRYPHFNLDLIIPIKERIIQQYGDQVKDKSNLKTVFRTNAGYATIQIPTKPVQGGVQLNTEARIFWEDIPYGLCILKDLSEMLGLQTPGTDKMIEWHQKFMNKEYIQNSRLNRSLLKETGAPTKYGLDTIEKVLGIQEVSQQIPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LEJ5</accession>
    <name>A0A8S1LEJ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0390124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LEJ5"/>
    <dbReference type="OMA" id="HNVTQYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="66" mass="7634" checksum="4832327493B19972" modified="2022-10-12" version="1">MKQAGYGGQTKPIFRKKAKTTKKVALKLECVKCKLKWLKVIKRCKTIVFVDANQLKKQQELKKNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFL1</accession>
    <name>A0A8S1LFL1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0360318</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFL1"/>
    <dbReference type="OMA" id="DSPDMRK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8064822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="205" mass="22957" checksum="86320F1EF87A473F" modified="2022-10-12" version="1">MQHRTEDYDLLYKLVLIGDSGVGKTNILNQFTRGEFLQDSKTTIGVEFASKNVMIDDKTIKAQIWDTAGQERYRAITSAYYRGAVGAMIIYDITKSLTFENVDKWMKELKENADPSLQIMIVGNKTDQKHIRQVATDCAIAYAQRNGVAFIETSAKDGTNVEEAFKNILQQIYKSAKVVKPTPQPTKFIVADTDVVKPEKSSGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJ54</accession>
    <name>A0A8S1LJ54_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0410152</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJ54"/>
    <dbReference type="OMA" id="FVWFGGI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="23"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8067760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="310" mass="35855" checksum="D0F33B6D00A75F76" modified="2022-10-12" version="1">MQEIGSTLYLTLLIIISVLSLIGCSVLLFDYFKQRFNANLAAKLLAILSISDAIYVIAVIVNPIAQNDGAFCAIQALFKQSSSVSTFIVNFFFCLTTYLTIVKNIDITEDKYYKYKRNSKIFSILFPFLIALVPITYNGYGIQYNICSFKTENAYWVGEFFLFYVPFGFLFGASLFFLLKIQNFLKEIQSNKLGLDDFRINRRTLITQNSQITVGEQYTRFLLYYTLVHLFCWLPIIISSLFNIIWKRNFLWFGRISYLCACSQGFLDFVLFMISKRVSSQSYVYERNNSILTADPIDIYRGSVVYNSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJ67</accession>
    <name>A0A8S1LJ67_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370244</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJ67"/>
    <dbReference type="OMA" id="NTRNFGT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="531" mass="62584" checksum="941996B0EBA77F3E" modified="2022-10-12" version="1">MNNHSTQIDILPKLITNQQKLLDPIHIRNTQQRHSQNESKILPHIERKSMLQGNSKQIGVQRFMYSFDYNTRNFGTSCDRGFQGLSSYHLSQKFGHLGTKLQSVDKNIVADLTDGEDVEEIKKDERLFYKKIYKYNQMPVLALIKSFEIDWIKEKDDQVNRKLSKSLILNFFTALQILNTHQDFIEFFMKHLQLLELNTVTLKQKGLHQLKTKVNKFKNEEFPASYLIISNQSGQGYFKINFPKIEIYFQEYQYVQTIQLSPMKLYELVNNNFFQIAEIVSEINVDFTILKQKLLQQIEYKAKVEQKIEEKMKPQMHNSDQIQLANKIESQQKVLVTKPTIPDVAIIISEKKSEFPEKILNKRMIKCVAQKDEKNYLCVDFLPIQFIIKEKVSQKTIKLYSPTFKEFNEFLTQLGSKHVKQIIDEYLITTFDLKPEDFDYKEFKKGTQISFSFPTFSELNLEITEEHLNNGLLSVFVKEIEINNKDLQIIMEPCTFGIYNQITCMTEKRNCTSKELTNILQKKYQVKFQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LN91</accession>
    <name>A0A8S1LN91_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0380226</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8066016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LN91"/>
    <dbReference type="OMA" id="CNIEDEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="3">
        <location>
            <begin position="696"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="449"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="567"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="468"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="499"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="519"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="571"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8066016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1048" mass="124997" checksum="6D12CB2B7B3BB366" modified="2022-10-12" version="1">MNEIAYPQTQFQRYSSRQINKVTYYCQSLNAPDKDILEKKNQIYKWLRNLPKKQLEQVLSFTSYYRVNCFLKMFKQDQNQLHEYWELDQSPFVVDVKIKLNEQYYVRECNRINMTNQWIYAQQYILDHTYISDYEWLFDTITIKGNIDEIIQSFEILSSQSIFTKSWRIETNEDEEYSEINFDLYQKQKAFRTLSEHIVDEIEKAILIKFQQFEQKCTYTKQDQLINFHQIELNFNNINYDIETQEYFKILEHSKDEMQFIDEKVLLQSWLQHIKCENLNLNDVEQQIQHLLQDKDQIFQSPLKFTISESLLFCKYLICKQLKNTYPLKKPVEENLKEKQKLKQKFSDLKLFQGKFDQLKQINSYFTSSEKSKILPENEQRLFSTNLEEKEQQVYEYSTNFMKKLIETVLIELGNYKQYKKKHKKVKNAKKQSIVEIVQPEPTIQVSTDIIKDDEWNDKPKSKKQRKKQIEKQRKKDNLERKKVKQMNQSQLKNVQQNDKIEISSKSSIKDDNEDIENDNNNNNNNNNNQEYIFEQQQQIQIIEKVIQEIPIIINQLPDDESEYIEVQNKKQQKKQSKQKQSKNKKQSNKKDKEFKDNQSLPDEQQYIPALKKSTSQSVQKNNNHQYDNLKLERMQSQQQNRIILQKKENGINKIQRSGLVKSKAQQKEQKLYTYDLDQNQLQKIYKTILEQKITNDVKQIYQKEIDSFNKYKIARNISIQRVQHVIKSYFKNCDTEIFGSSTTGLALKDSDVDMVVYGLQVYTKQQLFEPMRRLIEIFNELQWAVQCKHIFQASVPLIKVLVDPSIDFLSFKGEPKYILMQCRNLDLNLKYGDPSQNIFIDITFELTPPYAIYNPYIQAFNIGFQSTQYTIDICDKIQGFSEVAIYLKKLLKIKDLNDSYTGGISSFCLTIMLAAIGQDHNIGQKLINFLHKYGCNFDPNKWAIYLDEKGQNNFCNIEDEQSNQPLTIISPINLQKIQINVTKIQTILQLFQQLYIEIMQNIDQIESCLQKKINQKELLDNYNSHQIQRLVDLVFWQCPNLLESHIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQ54</accession>
    <name>A0A8S1LQ54_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0420016</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQ54"/>
    <dbReference type="OMA" id="NHENIRQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="408"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="665"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="667"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="681"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8068161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="814" mass="96385" checksum="56EF0D2DECC57A7C" modified="2022-10-12" version="1">MTQISQEYNLEYLDKASDITKVQIQQQRQLITIISVNDKDARDIIYYLQDRQSIDISQISLKKRIKQFILSLPKVSNPNSTFRLMWELVSMIFIFVQMIQIPLVLTYSVELSFGFQIFNQLMDVFFYIDMAFNFKFAYYYRGEIIYDRKKIAINYLKLWFWLDFLAVLPYDQMFNAEESQAQLIKIVRLFKFIKIVRLLRVLKISKILQKLEESFTLAQSFQEIIQFLKIACLILSIAHWIACIWNIIEYTDEQVNLTWMHKYGIDNADWEIKYISAFYFSVTTMITVGYGDINANTQTEMIFAIFAMVLASAIFGYSMSSFMLIIEGEDEKIQEQRIQNSKIIRYIKQKSIPKELQSRVKNYLEWLAGSAQIARNYEQYVLRSLSANLKTEIICLMNGRILHKVQLFSKEFTPQLINKLVYVLNEQILGPEEYIFKENSLDNDKIYFIQNGQINICITQRETLLKTLQKGDYFGEIGFFGRKPRNASAKSIDFVNVMSLTRIDLWEAAKPLESDLLKLYFMKDCLEVENNLKPLKLRCYICDRPHHIAKNCTVFHYRVSRKNIILEYFNHENIRQSQDRKINKKVKYNLYKIQEAALKYQTNQNRCEKQIKILQNDYIIQDVKIAFDIDKQKEYSEFFNEFNITNVAKNINFFADMILTEIEEKKKSRSQSRQTQKRLKNQTIAPRQSKATIGNMENILNNKSLENSSCLSSENSISCIDRLKSKSKRLLDLQSQYHVARRSLTNTTPKQFDLINNHNILVKKIKNLKHNRVSSVTRFTQNYNAELYIFKKETFMSKFIPQLLYNTKFNQFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQ60</accession>
    <name>A0A8S1LQ60_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0420203</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQ60"/>
    <dbReference type="OMA" id="INQWEDF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="13"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8068525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="312" mass="36418" checksum="29D368C741B187C1" modified="2022-10-12" version="1">MSQRKKKIEATPTRVALVCLKTTQNNIQINNQSLLKIFSQYGSISKILIFERGQLQTKVFIEYDEVDSAIDAIKSMNNTKILNQISCNVYHSRLKQLKLDTVPYTKGLDFTNPPSNTADQMESNEEQQEIVRINQWEDFQFDNNSTDEVSDEEEPQFSEEKMSDIITKLNQIDDEINKTIETKIVTALDKLQQNTMCIQVLITYDILNDMNFVIKLFCFFGQIKYLKQSQIYIYIKYGSNQQCQKAFQLLKDYLEIKIQYNFENVNGKLIQPDNIINYNSLDAIDDQSAIQELFQSYKIIKQQNNSLIQIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LT22</accession>
    <name>A0A8S1LT22_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0450113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LT22"/>
    <dbReference type="OMA" id="NHEIENY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="2">
        <location>
            <begin position="2"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="180"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="324"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8070209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="485" mass="57442" checksum="9F43424F71316C27" modified="2022-10-12" version="1">MKAFRYQVTLNISKVELSVKFPCTLQVMWKRGLNKCLTRTKEKTKNIFIVNEVLTLDFNVGENTQKKTHLIALLNVDGQSKLAGVVNFNINETMPIEGQCALNLERSPDPNAKIYFTYQVVNLGETDLSESRASESFHQKTLADSFGFDKENLNQRILSQKKSSTQQISNYEVLLTKELYEKSLKDLDQQKIKNTQLQDQVKQLSNEKSTYNEEVKKLKNEIIQLQNENEYLEEQFESSKQEIIKLKENAHQINQCQIQIEKLNHEIENYKIQNTILENKYQQTFKKAKQLEEKVEFSHFNKIERSTNTQFLEIPEYVQQNTTISQLDEQVKEYKIKNDKLTYDLQFTNNKLKTLEQELSQCKCQIIQYQTQQSQLKEQCNRFEIENTKLKMLIKQLEQDQNNKDKVQNKSQIDYQKQIDQLKDQLQQNIEKLEQVNDKYRQAKERIQKQNDIIEQQNDELTMQNMTFLKELQLIAKRQEQFGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LT63</accession>
    <name>A0A8S1LT63_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0460113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LT63"/>
    <dbReference type="OMA" id="CGLVFYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="94"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="354" mass="42438" checksum="8C9AC5A93DC7D104" modified="2022-10-12" version="1">MICYKSNFFSIQKYKIFMLKDEIQLSNDQHSFVIQLNSFEPKIMWIQNDNSEIISFELFLNKKKSIYKFAPNIGNLLKEFLNGKVCFQGINNHYKIAQYIQKRNYGSLVRMRSIYNEELVTCKILKQGKVEFEKEFRNEVVALQFLKHKFIPKLKEYYIESNHNYIIYEFIEGASLDKYIKKNILNTDQILKIMKDLLQVVQYLHNEGFNHQNIKLENIFYCSMMDQITLIDFGQQKSNDITSQRHIKSIKTLFTQDYTEMNLFTNGGFSFEKDFLDCGLVFYQLITQQILNNQEIINFYENKRFHHLVNEINNQKISKFISKLFNYSNLQNQNEIHFIQDIDEIISFNRDIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LU40</accession>
    <name>A0A8S1LU40_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0470232</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LU40"/>
    <dbReference type="OMA" id="IMRYGIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="45309" checksum="5CF313A1C716D914" modified="2022-10-12" version="1">MNNDQSLEDIDQFIHSYVPQQSIPNILSQAITQDQLLAQNVRLLQQLHEQQSILNLQSLMLLNPIVQTQVNHESKKIKKTNKTIQVIPSATNTKENAQHLLEIKGNVKNAYIKKITSLLSVEGDKLLDDDFHDDLEIESCSDLQKQDKLNGLLNSNNDQTQMKQKGRQSAKMSRLRKKVYLKLLEKKVSDLDQQISEYKKTTRQSFEYLTQILQSHPILNSMIIGNSAAIDQVMECSSPDQAQLILDSYIMRYGICGIKRRDYFKYAVKNIQKNFLKGNYGLLLMSWNNHINNYDQEFNHYVEIVKDEAKLEDDNQVYKILPTVDKMLNHRKLSQQLLLKFNQDIKNLRSLQQEVEETVNQFTQSLSPIQHLDFIKILEKQGRFNRFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LV27</accession>
    <name>A0A8S1LV27_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0460286</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8071079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LV27"/>
    <dbReference type="OMA" id="KEHLIVM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904430">
        <property type="term" value="P:negative regulation of t-circle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010569">
        <property type="term" value="P:regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090657">
        <property type="term" value="P:telomeric loop disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001352">
        <property type="entry name" value="DNA repair helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002687">
        <property type="entry name" value="Helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF34">
        <property type="entry name" value="REGULATOR OF TELOMERE ELONGATION HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="53"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8071079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="900" mass="106343" checksum="F11A5E505DA88839" modified="2022-10-12" version="1">MKTPFGIQQYFANKQIQHQQLLDEESEYSKRIIIKEIEIDSNICKKQVSLMLDNTPVYFPHQPYEVQKIYMESVIKALNKKQNALLESPTGTGKTLSLLCASLAWLKKNRQDQLNFDQPKNIKIIYSSRTHAQLKQVAMELKKTIYKPNISMLGSRDQYCIRGDFNMLKGTILNQSCRKSVKANQCQFYKKEHLIVMAQSYSTLINSLEEAKQFGQKNKLCPYYFERQRLDFADLILLPYNYLLEKDFLDVVQIENSILIFDEAHNVQSTAEDGSSFLITHNHIVEAVKDLEKWIDELESVTVFYDQLKTKLNSAKVSSDLNEFKSIMITIRIFAQYLESFKNTQEFIITDKEDKYLILDARQIQFMIFENTQDKDNTFQLWSENSQNNYAKGVNKTNFAQYLIHCSTLIEVMGELSQIPGYHFESWVKFMKNVYDLIIVEDEREKQKLSLDSLQNEFNQYKLSFILDQSNQLSINMWCLEPSLAFSRLFNKNIYSIMLTSGTLSPMPSWVSELRLPFDVQLSNEHIIDLNKNLRVFQHKIFDFQYNQRNNEEQICRFGVTLLSLCQIIPNGILVIFSSYSLMNKFRSKWTYNKLIPRLSEIKACLWEPQQSSEMQNVFDTYKLKAKKGAIMFAVHRGKVAEGIDFSDELCRAIFLVGVPYPPKQDNHLLQKMNYLDKLFSDPEFKHQQRIRSSEWYIQQAIRATNQAMGRVIRHINDYGIVYLCDKRFEQKDIKQGLSKWLQPAIQPWINDDEIIKQTKDFFLNRKNQINEKEQIEQKPIQEQEVKKRKLEFFGLQNKYEFEKLKDETFDRMKQQINEQQNQNDYINLKNYQMNNITQDKQLNNTNSDKQCDIVNYQNSNYSNLDNSHIQQKQLKRDFDNILQIESNKKKLCIKLKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXX7</accession>
    <name>A0A8S1LXX7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0450195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXX7"/>
    <dbReference type="OMA" id="CNDYHTK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="237" mass="28034" checksum="156F5C0DD18D41C1" modified="2022-10-12" version="1">MNSNSKSIYKNVEFQDEPYSTNNKFAVYVPKQEIPKYSASQQVFYNQQQQQSPQKQLKVEFQDEQENRNLQNQIEQQQFEQEYQQSQSNQPKAEIQNHRNVTQNRYRLTNQTPKTAQGKDQQKFYSSTPSQSIIKQRTLQQFKNSLYFDERINKVPKSTIRNHIIDSIVSDADLLLQRRELMTSIYKLKQLNLLKNKEFAVNRLPGVGKSSFVYNDYHTKSTNNGYSRNFGGVFYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZS9</accession>
    <name>A0A8S1LZS9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0480074</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZS9"/>
    <dbReference type="OMA" id="YRPGQHP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="446"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="162"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8071832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="476" mass="56588" checksum="A79266D722C491E3" modified="2022-10-12" version="1">MRYSPASQQYGQAYGYSQPQQSARPPYQSYKPNDNAQSHTVTHPHVREMYHREAPITVVTRDEIESVWKAKCSQLEQALTECQTEVMRLRGIGATEKITYVEDTLKINQLYLEIDRLNKCLLDMSLELDQYKHKLYMATDKQARQQAEIDIDKMRSIMIENEAMLNAEIVRLRDQNEELSKRYRILELSMADSRHQLSLKEYEATLSQLRQEIDRAQFSINSQRAETEEWKLKYQRLEAQLRDSAQYEVEIRRLKEMVDNRNREIESLKLNSQFENDGKLQNLQLEIERLQNELRQKNDETNRLRSQIQQQELTIKYLNSKITELDNKILQMKQENDSLKANQMNFKPSFQSNNEDKEQIDRLRSQIQQLNIEIEDWKSKYNELDKMYQDLEYEYQNNNTTYEEVTKLTNDVSIWKNKFQELNREYHQVQEELVVTSAELDSLKKKQNELSKSGVRSSAASYRPGQHPDVFPQAQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M1L0</accession>
    <name>A0A8S1M1L0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0430033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8068854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M1L0"/>
    <dbReference type="OMA" id="QVFTRNY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035911222" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8068854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="43457" checksum="D5C07320C132B96C" modified="2022-10-12" version="1">MYKVIILCSLVLFASCMSEGETLLQSLQSTEFGKTIIQTIQVELQGNSSVDKIIDLLTQMKDRINGEQDVERQKSRDHTQFCDDKYDEILFVIDSSEYQLAKDQQTLPLFIQEQKNKQRQLLDKQEIENRNNQRIDELTQERDITRQQYEARRDELTNMVGALQEGKRIISKLSTKKWDPLAGTYSFLEFSQFMFNELEVHQKLLKKQSNGIGLLYELLLETSQDPGIQANQQGVAKIQEIIDELIESVFDLLKQELLTDNAREQDYQNQKERIVIQNRRLEATIATFKARVLIINQTILELNNDIRFNTEKSTLLRKQKDDWERTCVDYHNGYVEATKIRTQQSDILTEVIQVFNRNYNDFPSLIQKISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M1M3</accession>
    <name>A0A8S1M1M3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0520186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M1M3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="210" mass="25465" checksum="421B4D3F5C031310" modified="2022-10-12" version="1">MELDSDTMLQMTYVEKCQWNFLNLSFKSKFCQKDCFFDKYNVSYYLNWILYTSWYDISKFFFHLILNLSILKQDWKILNYQHIVKGFHIYKRPLCRDSQTNYQIEIWVGSIFQQVYHQYGAIRTQWDEWFYQSFKLYKIFVSKKFDVNKAKMLNSISELKFSTKILGNLNFDISTSLTHLKLISTRKCQQGKNQKWQLCLKMIVSSLLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3M0</accession>
    <name>A0A8S1M3M0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3M0"/>
    <dbReference type="OMA" id="CNYISQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="266" mass="31473" checksum="D598952F47032E8F" modified="2022-10-12" version="1">MSKQWQIHQSVQESLCSSLNKDEILEEFLNICNYISQEDQNSIKSLNSKIQTIKKHYAQQKCRIEHQFKYIQTLLEQKKKELMTLLNTHEQELDKTFKNLLEKVTQIQSVIFNIQNDILSNRQEIVSEVDDDTFHNILSQFDQEIQMTQNYKKEILSQKITLIIIEEWTSHHQDVIKNLLNQCITIKDYQKQIETQHSELTASTSSDKFYSSNRSSQEFCIKQQTPLIISFDQKYFLTQKQTEISKSDSDMHDSCESDFLKELTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M479</accession>
    <name>A0A8S1M479_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine Rich Repeat family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0470116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M479"/>
    <dbReference type="OMA" id="MFAIVEE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="941" mass="109578" checksum="6651DC6D7FE3FB02" modified="2022-10-12" version="1">MIEESNYHSQQDNKYQNQLDRRRSSAQFQDVSTMINPNSTHLTRNASYSMPHESQINPQILAKIAAMVPYNSDNKKKFFRVDHVLKLKKKPDPLEEEQENEIRKNLQLSPEIVKEDLQNKPLVGPQAVQLYYQHYKKVNKVRQQNDFFKISDSVQTAMLRQAEQIDVLPCKIGVVKTKGSEQSLQISHQKYGDRYASMLSEGLKVNPNIKDFYMANNRIQSSGATQILNQIGKVAKVLDLQQNNIGKIGTDSICQQIQSRENKLEVLNLEDNKLGDRNVTRILKALLNSNNKLKSLNVSKNYLTNDIAEILKESIIQLDNMEQLYIHWNQIKGWGGQKIFEALIENKTLLVFDAGWNSFGIQERKDVQCIQKICDFLITNKNVLHCDLSSNQFSLNDCKLIANALKQNRTIYGFHFVNNWGIVDARGFLQIQENELQRVLGETPRIKGLQQTTTIRQENVCWICEGWQEQKFIWIPDVSGEGEADPLFIHLDFEAYKQVYIPKNQDEISLTLMIPTNTTQYIYTMTDTQLIANDQSNIPYGHQLKINYNNNYITVLMENINYVKKDKHFLIFDQVEFKPQINVLPRTPDPVYIPPKLKKQKRIWTFPISIWAKDFKFENEDLLKKCFEKDFTCSKISKVVKNPDELLEIKNVLWAHYKLIKETYKQYSSNSPTGDIWSISSNVITEFAQQTELIDGKTLKLSDLDLKFIATCAASIEYKGNFRNPERSLCRFQFMEFLVRVSEDKYLKTKVANNMLEATKMILEQCKSYMSTFDAQKWRNERYFNEQCDDCLKFFKPLLNHLYNKYSVKKVKPGQKKFMCLDEIHEICSQANLYDERFVERDADNAFNLSMMLQVDELESDRIFQMTFIEFLEAVARIADKVSMPGIDAVDLTWDQRVSQPLHLKLEKILIHLANTCASEEFKLQYGNINKSMFAIVEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9V9</accession>
    <name>A0A8S1M9V9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0530048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9V9"/>
    <dbReference type="OMA" id="CDERAIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="531" mass="63778" checksum="2B3236F18CC6545C" modified="2022-10-12" version="1">MNQHTNIKLKYWNNYNLYKLKLYLLLTQKNLTKNQTQLNRSECKHNYSSEEVQFKNLNKVHNNLKKIIIIKFKLSKLINYQQLKRTMKRSQTAFKKDFGHFAKQHRSKLFLKRANQAIVNNFEQIRKQKSIITSNINLIGKQHLRTIQVKYFRTNLSKVLFIEKEMTAIEFIVLALKEYSLERRFDQNLYEYKNYTLAYQLETLEDQMEMDEDKFRKPIQRTKSYIEKDDDNIAARRVSCDERAITSQVEYSFADIEDRTITIDYPATLSCKQVDIVVNSTFQLQFGIDLLTLKKDYELFPEYIILMVEDVQTQSNQQIKCLNTSKLENVFDQIKIGSTNKRSYNDYYLRLKYPCIHTDISPLSLNTPIKELPIHWLVLEKKYSIETQTTDLIDISNKFSNFKQPNNIEDGLLSYNPQECQCKIESRHYLSSIYSYQEYALMQLKKDEQYQVIIGIDYFDIRIIYINQQNNLSQNYSLFQYVQESQEIRENYQNKGNQRKFFRQKIKQILFHIQEQCPQFQIIMLYSRIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9W3</accession>
    <name>A0A8S1M9W3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0480201</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9W3"/>
    <dbReference type="OMA" id="KAQFNSS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="313" mass="35432" checksum="719D06C975AD3646" modified="2022-10-12" version="1">MSLLLLNASNRIAQGFLKVAAESGKYEKIICADIFPTYFTVQRLLKFKQQFSTNIELFKVGDRQDLHDVIKQANNLLYVSHDYYQVTASKKNLLVATLDLVKTRNYKTVAYVAPVEHDHQEEIDEWKHLEVEGRRSIPQLVGIRSDITFGPQSTFTNKIAQRIYNGQSIYFKSTGQSCAPIFTGDLEAIVARVLAGDHAGKLLLAKGHKHIDFKSIIHLIEDSLGNNYKAQLNSSFIEKIIHPTNNCIIGQQLYCPSYINLTKLIANYKALENTGYDQVVGDKLVDIEDYHKNNKTVVDQSLKADYELSYLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9X0</accession>
    <name>A0A8S1M9X0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0530008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9X0"/>
    <dbReference type="OMA" id="RCNPEDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13969">
        <property type="entry name" value="ADCK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045307">
        <property type="entry name" value="ADCK1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF19">
        <property type="entry name" value="AARF DOMAIN-CONTAINING PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="2">
        <location>
            <begin position="121"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="502" mass="58519" checksum="C9402214464CD35E" modified="2022-10-12" version="1">MIKSFGYLVCGAVMIPSITLAGSTVYLYPEFRTNPAQFWLAFQRVYRVVKAGTNMALAYLTQPDQPMDDKHYYGALQLRQAFQQNKGLYIKFGQILGSLDIIIPEPYRATFEVMCTQNIETPFDDIERQIQVSTGKKIEELFQTFSKKPISSASIAQVHQAFLKDGREVAVKIQHPWLKEQIDGDVKLISLFTDVAEFIFKGFKYKWLAEELQVNLPKELDFHGEINNAKRIKEILKPFPDIYIPKVYEEYCNDRMIVMEFIHGTPLSDILREKEKHDFNYPKIAHTISTAFAHQIFKHGFVHSDPHKGNIMVRKLNGKQQVVLLDHGLYTTLTEKTRLSYSLLWQGILEMNLDYIKQATNDLGAAQNYKLFASMVTSKRFSDLMNTSMNMQDRLKRPKDQDEIAILIQKAAKKHKQITIILNQIDRKLLMLFKINDFLKNIEFRLGNPIDSCQITYKYVSMVNYEDKVKKQNIFRRMMYKFRLLLHKAKFEILSIVMSIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEB6</accession>
    <name>A0A8S1MEB6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0570018</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEB6"/>
    <dbReference type="OMA" id="NLIRVKW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="116"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8076711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="439" mass="50902" checksum="DD5FEF5B485D20CE" modified="2022-10-12" version="1">MKQHNIEYDQCLFQCKTKKKQFIGKENRFIYVFEDRLIINRNQNKGDPSRVIMFENLIRVKWNYSINSKTFKTFLKSFTLITNKEQLKYYADFEMLIALKKILQKFVQQSSIEDEYETQQVLGEGSYAVVFQLKNIFTGQEYAGKCIEKKKLDKIDKGLKAVMNEIEIMRILSPHAQIVNLHEVYDGENSINLVLDLCSGQNLQAELTKRNSMLEDSEVKIIIHHLLLAVEYIHSKGIMHRDLKPENILFQRPQDFTTLKIGDFGLSAIQTDAPYLYPKCGTPGYVAPEIANLVEKDKEYSRVCDIFSCGAIFHLLLLGEGVFPGKGHLELLKLNKECNINPEDKRYNALTIEQKDLLFKMLKTNPQHRPSAKELLNHPYFTNQKLNAVGIFSMNIIKYEEDNTLVMQDLIHSPTKVGLEKRFSFLQMSNDRNTPLKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MGD3</accession>
    <name>A0A8S1MGD3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0610157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MGD3"/>
    <dbReference type="OMA" id="ARHKYLD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="100" mass="11560" checksum="61080D9DE742BDCC" modified="2022-10-12" version="1">MKARHKYLDDHKSLQDTLPELRQYGQIRMLSNTLAFTFTAGFCAKVFSKKKFTKHILYNVGLVACFPLIAVVGHWQDEQSKIAEKALHDHINLKFQSKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MGI4</accession>
    <name>A0A8S1MGI4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Deoxynucleoside kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0570059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MGI4"/>
    <dbReference type="OMA" id="CVQDRTL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019136">
        <property type="term" value="F:deoxynucleoside kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01673">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004279">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002624">
        <property type="entry name" value="DCK/DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050566">
        <property type="entry name" value="Deoxyribonucleoside_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031314">
        <property type="entry name" value="DNK_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513:SF35">
        <property type="entry name" value="DEOXYADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513">
        <property type="entry name" value="DEOXYNUCLEOSIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01712">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000705">
        <property type="entry name" value="DNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Deoxynucleoside kinase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="246" mass="29470" checksum="AF91CD98F6B29C10" modified="2022-10-12" version="1">MDKKFIIVSLEGNVGAGKSTLFEILRQEFPKAIFLMEPLEQWQKVHGNSNLNILEKYYSDIQRWGFTFQIYAYQSRLMAWDRQLRAVLKEQKLQQIDNQFSSPSTNNDDEPILVFTERSIESARELFFKLCYNDGTINELEYHIYEEFYEWLMEHYKQYLVDCVIYVNTPPETCLERLTRRGRQEEACVPLDYLRKLHQRHEDWLSDTNNKFKIINIDATKNYVKDLDVKEAVRRQLIDEISTLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MHI6</accession>
    <name>A0A8S1MHI6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0570160</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MHI6"/>
    <dbReference type="OMA" id="ICWRSIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF388">
        <property type="entry name" value="GOLGI-SPECIFIC BREFELDIN A-RESISTANCE GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="4">
        <location>
            <begin position="52"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="520"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="379"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8076995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="782" mass="93002" checksum="219E13A2556DAD52" modified="2022-10-12" version="1">MLDDDEDQVIIVEEDSPREKSKPNQKKQYERQQNNTDKILNGNNPLIQQITICEKAVQERFHIDQFITRFSKDQNSAIQYLIENQVIENTNEDIAKILMTTKGLNKDSINKYFCKPDQKHQDIFEAYCQLLNLKDKSYLDAMRLLLQRFRLAGEAQMVDRVVKIFAKVYNQQNPDVFKSEDVPYVLAYSFIMLSTDAASTKILAKNKMTKEQFLKNNIPVFPDISPKYFEEVYDSITKEPFQTTLDYLEQMYNRMILCNEKIQGEQITKWLQVAFDLMKGCNLVKYGRYNGGQPRKFFLSSDEKRICWRSINNDNEPARYINMSDVHDIALGQNTTEIMIKNNIPPEFDMFCFSIITIHRSVDIKVNDLEIKSKWINYLRAVIINRREMEAKRAEEKQRRQENEEKRSEIWKNDILPFWRSHWDYEPNKPLNYKKYISVKKEEAVQKAQNQANSASVLESLCRCLKKTISLNPIQNRQQIQNQQYSNQSEINNGSNIQQEMIVQGKKNKSMLLMILWKLGLPDFARRTLWPIIIGNNLKIREELYSIFVRKTTIIQEAIRGDIARAKQQYPFINDVKAKELSNILHAFSNYRPDFGYIPDLIDIAVVLIKHLQEYDCFQALVNLLHQYHFLSVFQNDTRQIEWRLRFFEENLQRILPFVFSHLKAIKLETKLYLMKWFLKIYLHQFKFPMLSRLWDNFLLEGEIYLFKVGICYLKYFQIELKMSNLDEVVRILTNWQPEINEDYFFIQIDEIPIKDDDYTKFLEQQKAAVLNTQIHQTLIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MHW2</accession>
    <name>A0A8S1MHW2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphatidylserine decarboxylase</fullName>
            <ecNumber evidence="3">4.1.1.65</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0590130</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Phospholipid metabolism; phosphatidylethanolamine biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.65" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MHW2"/>
    <dbReference type="OMA" id="CRHYGVI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033177">
        <property type="entry name" value="PSD-B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00163">
        <property type="entry name" value="PS_decarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067:SF6">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE PROENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8078031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="328" mass="38116" checksum="E4816E9BED5CBDC9" modified="2022-10-12" version="1">MSILEQALKKAVGLGYMKWGLIFGFANGIAVYSYYRENRNKYDLEAYQQSVTRYVSYQSGKMAETYIPKWLRSPLFSLFGYVYDVNYDDMLESLENYENFQQFFTRKIKSREFDKSVNKLIVPADSKVLSFCEVKDDSPILVKNVHYKLGYFLTGQETFEMTPQILNDAKKRQNTKLYSVIFYLAPGDYHRYHLPSDFQLKSRSHIVGHLAPVKISYISNTPKVYETNERVSLFGTYNFGLMSIVLVGATNVGSMTLNYDKEFQTNQKAQELFVYKHYDPNILLRKGDELGMFKLGSTVVMMFEAENVKWNIEEGQKCKWGDVFAEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MMV4</accession>
    <name>A0A8S1MMV4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine Rich Repeat family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0600098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MMV4"/>
    <dbReference type="OMA" id="DMIQEWR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8078533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1203" mass="142816" checksum="E49F96343A77A99D" modified="2022-10-12" version="1">MKNSNERSKCQLPRLKDYSKEKAQKLKSFFKSNRSISENKIAESKFKTKKLLIDNQNSKDHSLMAYYIKSSEKLTEMRSILTNTSVQSDQGIVKKKNFPLGLLKHRFSPIEQGKKETQEINDDVSKELMEAEQESIKSGNSIEFDEDEYIMSDISSEDEPKRFNGIELQNEIKKIFQKLNQEIDQSYFYFIKRQERLKDLAINFNKKNVDPIHFYCLNDNIFPKRIDLSNCLVNEKEMHFSDLGLTEKYFPLIKNLLQSVKSKTVKQLFLCNNQLDDKSLLYLIEGFPLSLKEINLSNNVLGRRGALILSNHIQKFTNLKSINLQNNLLTDKGVSVLLNCFQKNVTLLKLNLSENQITDSCSTVFYQFLLQNNYLEILVLNWNQLGPTAGTMIVKGLQQNRSIKVLDLSYNHLGQNDRSNFMQALCETISIPQITLAHLDISYNQLSEKQLKLFSVALQKNNTLYGLHIEGNKCSAKVNPYGFIQFTNDSDEFKVVQQKKTAIDGVNYIPLQGDNLFGNDCCWICQGWMEYRFQYTPENDNNQDPIFIHLDFLDYQPIPMTSSYELRQQLIEAQKRKSNYFDLTTGEIIHQLKQLVNTDKRITLAAITEAYTEETSKKNEEITKQMILELSKFYYTTYQMCPPKRKILYFFSNPIKEEYFIDPRVMNMPAPLDNAILQGKDPKHAVHIFSDGTKLAFTKVQQVNYIFSRQEYIIDDKDNYKPLIKVFPRSSQKKYVLRRFANFLVRKQAHLITWHKEDSIFRPFNGDTEELLNDCFEFDWNCSKISRFVKSEYEKLKLKEYFRQHYQLIKDVYKFYSSVGYQPPNFDVFCIQFPQYMKLLNKLAIIDGDLLKSQDVDIDIVSLKNNVDQKYIYNPEKAIIRYQFLEMFFRIANDKYVRTDIYKSYADAIFRLLKEFKEQYEHFDMIQEWRTQRLWTKECDHLLQIKMPFIKKLYDYVTDITNRKWYFKLKWISIREFKEFCKQFNLHEYLSEKLQVIIYNFSMMTQIDELTLDRNIRMTFIEFVEALGRIAERISPTPIQEHIDSYTLLQRQQLPLHVKFETLLTYLYYQMKSRDYEYEQFCDLFVYFKLEQSPKSSFPKIDKSKKLDKDHDYHNDLTSLTIYNAVSYLNQNKQPYLNPKYKQFLKQNIIQTSPIRPFQHILQSEKIKRARLQPETYNFESRLNQQQFKAQSPNKQITLSQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSZ2</accession>
    <name>A0A8S1MSZ2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0630241</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSZ2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="35" mass="4033" checksum="D49E1AC70A89899F" modified="2022-10-12" version="1">MGFLLHINRRIQNAQGKKNTLVVLIKYHLNIIGCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MVI1</accession>
    <name>A0A8S1MVI1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0670022</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="MKSFETH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045162">
        <property type="entry name" value="Vps15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583:SF0">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="952"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="1351"/>
            <end position="1384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8082021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1384" mass="163497" checksum="4CB8C782C615AC90" modified="2022-10-12" version="1">MGNSLIDHRGKLNLMKSFETHNTEQDIGVRQKRLSDNKLFKTMHYKMHHQNLIVKVHLLLKQLTHNELYIHYQNFQYLIEKLEPYPNLMAFRGLINFGKLEKPSPQEMQNECNKDYLAVSRQMLYMTLEERLQSNYKLPICLLHFYTLQLLSAVKTLHQQNLYHGHIRTSNILITSLDYLVLTDFASYKPYYSNEFEVIRNVYESSIQKCTLAPEKYADALEIQYIANMTKQQIYDLQKMDMFSLGCVIYEIYTGESLFTFKQLLSYKRKEREPLIQVDGFIGDLICYLIDLDPNERYSAEMAFNNFQTFICADEIFEFLQKICYTINFKGEFMYPDIRIQLFRQILSSTQFKQWNYVMPIPIQFISLNSFNDLDIVSQYSNRFSQIKFNIPQSCDTLAGQIENNENLIIKNNIQNQEDNSIKGNQKQNINELICWKSKSFDRKSLLESVKINNNKRQNEYIIMILWIFNQMRNCRFLQTKLCGLELVEYLLEFIDDSSMYKLIIPNLGLFCEDYEGQTQFYAFSILLDLLRRFNYPHSNKTESLIFQLYIWEQIKTRLQDMNQNPMLALKIGDIAEILHIFNRKEEKNLYLQIIQKKLQDDNQELSILLIQNIKNVLKYYDSSQTIEIMKLLIAQLNKIHLKIPLLEVGFDLLQYYAKTDMSKTLLTCLQLNTRNEIALFRTLRIVTKASEQQLLELDKIKQFIKEISPLVLHHNRWVRDEAQQFIIIQLRNFNSLDNYLHILPHIKKYLKQDVPHLNEEVFTQLIQKPVRQSLFINQNQEFNNNSQPFSEREQYNYNNYYHEYILKSKQNLEISAQKLNNNQVLPSTSVKQRLNEFYEAAYKLKFIKQESYNRFQEFIKKDRTLEDYSNFSYDYFKIDLKKFENNQNFNKKYLVSQSSTQWINNLNFNIIINSFIQKSYQLNKSEVKFQRSIQPKIVRQFKLKNHLVTTIPEHDDIVTSLQKHCDQSRFISGSHDGTIRFYDMNKIQEDFTCGSIKQITVFKDQKLIDKVNALCFLEGTDSFIVGTNSGQVSQYQIDQRVTTFPQADSGIRRIVNYDNAFCYVTEKGSLILEDIRSRSQQHLSVNKKCGLASSLIHDNQKTLLSTLNGYLILYDNRCMMQMNIYQLLSKDDQPLPIYNLCEMNRNSLLEGINPNSDKLIGIGYQSLNNEVAFFDLKMNKNTIIPTLFLHCSQNKNIQMEMPKLKSVLNVEQILNNVNKISTYAQLSDQTIQKFNLNFSSIFVKEQLLQNMWKNYLDGSFQDTTSVTSLLCLNNRGYNQKLENTIITATKDRSIQSWTLNSDINNSELEFQTNVICSPYNSNRKFKQFIKGDVFIIEDHEIRNGPNTSNLIEHKDSVTDMIYLDNENSYLLTASKDHTIKIWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MY16</accession>
    <name>A0A8S1MY16_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0690049</name>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0690050</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8082979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8082981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MY16"/>
    <dbReference type="OMA" id="AFMLLYD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF47">
        <property type="entry name" value="TRANSMEMBRANE BAX INHIBITOR MOTIF CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8082979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8082981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="255" mass="29263" checksum="804623927DD8A35C" modified="2022-10-12" version="1">MLFESSIRSGDPRLSNRNKSGQATEVQNAYDHSFNGRIIYARNLYSKLFLQLIIVCIYVWIVHALQDLDHFLEQTKWIFWLSLGVCIGISTLAFIYRNQIKTAPINWLVFLSFTLSLASVCGCLVAFGNSQIGLLIFVNFASLIFFLFLYSSTIRRKITYSGAVLFVSAGILIVFEMFTIYTKISLFWIMVISLSSFLFAFLLLYHTYSNLNSEDSYDISTADDVSGSVTIYWDIILLFLKMAELIKDNLFNRHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MYD1</accession>
    <name>A0A8S1MYD1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0670248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MYD1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035847364" evidence="2">
        <location>
            <begin position="17"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8082426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="181" mass="20551" checksum="B3BCE03660CAF914" modified="2022-10-12" version="1">MYKSVLICALLLTAYAGHVRKQHGAAVHQKRTFNSAFMEFVNLGDSDYHLNPKEAQHWAQITSEEVKHETKKHKSLVETHAEYIPGVVGQVTDLSNNAGVYSYTVTDSNGNIIEQDSGDHLAKQFNTAYLQMTQEIERGPAMIKLQTDLDNIMREDQMEQERKEEEQRQALEGTALSEQDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZX1</accession>
    <name>A0A8S1MZX1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0730041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8084892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZX1"/>
    <dbReference type="OMA" id="MASQMIL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="59191" checksum="1324E87FF60EDE44" modified="2022-10-12" version="1">MNKFTLTFYHKDLEMQYQTTRIFMRKRILYTILFSYLIMNTMSYVQNIIEKSNEFLIQLELLLSCFLVLLYLIYCYMDNNFSYYFCVLNVVGCMMQFQLLNYQSGQKIFIFGANLMASQMILLQRSDFILSVIQIFIIAFTRITLLILLEEIDYLTIFTTISISIFLAFVQFRSDKNRRQQFLLTLKNNHWDEYLPSMIGKPFFYFEYDHTQFLIKKIHRQKDIPIYKDELCEGCNLRNLLREYSYQNQTLESYILKRAKSRVPLIQGFEMSCQNKKKGILFIEYTEIFSDAHIYIITIIQQSKNSNKVLIKQKQAFQVYLFKYIKNLMKIFNHRNSFTKIVNLNINYICLLYQQDQTIKRYIPKSILLKIIKLINNKSRQCNIQIICDQDIEIQGYKYKFISFWLQIVNLAIQINRREQIQIIIVKIDCYIEFVICLSQMAKQSLIFWISQNKFMQNLQNELFWDVDINNLRLKMFQDLDISYIQYLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0L0</accession>
    <name>A0A8S1N0L0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0760095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0L0"/>
    <dbReference type="OMA" id="YCEKIAI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8086278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="314" mass="36040" checksum="C86B2C6F246134D5" modified="2022-10-12" version="1">MYQEFTTLINGMSNSIPKKNIAEVYKHNRQRQMNLVQRKSWAPSIKYSMFQSNYGTNDYQIVAQESSDSESIVFTPSQYPSLHKVASQIIMISQYSQIPCSTVLKDGLKSKLIIKNIDENIEIQKVQEFVDKLPIKGFVQEIKLIYYQSNPCLLFQLKTDQPLIVANQFMSKQNQKQSKSIFGYKFSCGQLALMNEDFAAIILRGLDKTISVSQIEKIISYTLKMDQNECIRETQKHIIEINDIGCITIVLKDLEYCERIVIALNGTNLNGLQKNKILVNVHPESVKIRKIDLSQSIQKGLLQKIIKVNEDDMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N751</accession>
    <name>A0A8S1N751_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0800211</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N751"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="403" mass="48489" checksum="CC4A6A0835D5FEFB" modified="2022-10-12" version="1">MLNLQYVTEEVYTFENEEDIIKAISLDQFQPGTFNPLNKSISYKINHNKCIRLRDLIFWIESDFIRIHLGQLLYLILSILQKVEDIENKGIEHNYLDLNRIWIQLSEISQYPSLIYQFLDYSIHFTGYNCSLKDQDLYQQQASIKIKEIIKIIIDKCFNRIQLKKNNNNQEIRNQIYQQILQPIIDLCNSNQSINIIIEYIKQKLNDYNYQYNQNNKLIQSLNIDDNDNDYIGSKRYKLIQKVNNDIKQIIQFGQKYGIIIIEYLLNYSIPRIANNLKSYSLIDYQKKALKQQNIQNERYKELTNMIEQHIQDGVKYHLDEYSNYYKFDINQQEIKQLEQEIINSILQSPQVKYFYNTYWFQQNEQIVNYIFAAITKISKKEIEDKLETLFIHKILLLIIDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NB81</accession>
    <name>A0A8S1NB81_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0710073</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NB81"/>
    <dbReference type="OMA" id="DWNTIIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="35404" checksum="4990E1A4781E6B16" modified="2022-10-12" version="1">MSLLLLNASNRIAQGFLKVAAESGKYEKIICADIFPTYFTVQRLLKFKQSLSANIELFKIGDRQDLHDVIKQANNVLYVSHDYYQTTASKKNLLVATLDLVKNRNYKTVAYVAPVEHDHQEEIDEWKHLEVEGRRFIPQLVGIRSDITFGPQSTFTNKLAQRIYNGESIYFQSTGQSCAPIFTGDLEQIVAQVLAGSHAGKLLLAKGHKHIDFKSIIHLIEDSLGTTYKANLNQSFIEKVIHPTNNCIIGQQLYCPSYINLTKLIANYKALENTGYDQVVGDKLVDIEEYHKNNKTVIDQSLKADYQLSYLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCR4</accession>
    <name>A0A8S1NCR4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0860066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCR4"/>
    <dbReference type="OMA" id="SQQDYQV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="452"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="63003" checksum="5DFC8E98549292AD" modified="2022-10-12" version="1">MDKNGESIFTSEFWRDINEQYFIVQSPVQIRDIDILKSKAGNRRSQKCLNPLNKLIRQNNRTKLFQTNLNNLKTRIQLIFLKRQQKQEPIQNLMDEMIDQVYFNYLSNGLQDAQELIKNISLCSDAQQLQIYNKEADLLSNLMHQIDWSDELSIAIEEHQQQLNEISNNNKNALQILEQHIHQINLTVQYTYNSYQIGEHKLKLIRQRITNHENELNMLKQLIISQQDYQVAESIGKATINNYNNTITVIFDKYLSQTPVTLSLEEFDNLQRLREEPYDLTNQQSNLRSDDVFEMQMCHYCKQMVDIKNLKQCQYNHYLMGLHEYNEDLLICQRYQINGKQIQQYLLDLYSENYIIENEQIMCQRYFCQNCLKFDFDSYETTSYLWICPLCKGLCTCQRCQRNDIIYKMKRQFLELNGDLEDIYKSSYFESLINEKRRQIIDIPLEFMNIPKIEYEKNIQQKSNKKQNQVNQIKKKINKVDSNIIKKCQKLYQLESSSSSIKIKRSKEITTQNTISSLDSINSQMYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDF7</accession>
    <name>A0A8S1NDF7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0860171</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDF7"/>
    <dbReference type="OMA" id="WINLNEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042234">
        <property type="entry name" value="WDFY1/WDFY2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189">
        <property type="entry name" value="LD41958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189:SF1">
        <property type="entry name" value="LD41958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8090710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="500" mass="58348" checksum="133B9BC62B72B1E0" modified="2022-10-12" version="1">MQNHSFCKLSVPTFKQYEQFQGVTLYCIDVQVIGVTAWRLELRYSDLFDLNTILKAQFQKLPKFPGKTLSKLTTTQELEKRRIKLNTYLSSISERLEIVTCPIFCELFEIAKYAPKYVFEQPKIINSQQHLQTIKNCQYIKEQEALLVVSSQTEGYKQLIAFMQNDENSISEALGQVELFSLKDNQIVLKWSQIFDNQAQCLYYSEGALIVGLEKGKIIVYQLDQDLNKPQHPSNYQMHKMRVNGVFYHKLKKVIYSISQDGTFVVTQRDYLKHKDQLLLKGELTTLLVCEYREIAFIGNSKGSVFMLDLNKENVQYLKRVDCQVLSKIVDFAINNQQGFLFAACESGYLQVIDIGSIGRESNSKTKICISTNQQTKKISYCQQRHHIYIGDTLGNITVISEQTGCPIYSLRAHELDVMITLVYYLEESNKVITYGKDQMFKMWEFPTWINLNEIKYQEYQEIKQEQLNKQQTKSKLQKNNQAYQEDDEDDLNDWHKNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFS4</accession>
    <name>A0A8S1NFS4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">B9 domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0870032</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the B9D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8090922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFS4"/>
    <dbReference type="OMA" id="FTAPFEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036038">
        <property type="term" value="C:MKS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010796">
        <property type="entry name" value="C2_B9-type_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12968">
        <property type="entry name" value="B9 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12968:SF1">
        <property type="entry name" value="B9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07162">
        <property type="entry name" value="B9-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51381">
        <property type="entry name" value="C2_B9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8090922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="238" mass="26988" checksum="54EF6AA2EB3F5EA9" modified="2022-10-12" version="1">MNTRNYKKDVSDQGSMKGQTNQNFGYSQPQQFQDGISRIQEEMMDPRNIQPDSMFVNFSGVLECGECEDEESLRIEYAIKYGGDDWTYLKTSNNADDAEPSGISQLSTSRNLHSRNIVWNFPFECNFQTKNIFGWPQVVVRLSGPDFMGRSVAKGYGSVHVPTQPGYHERIIRIFKPLPISGFTGFLGYLLGNTAELKNFDKVISSGEGREVTRVKSVGYVKVKFHVTLVNFDKFGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGL7</accession>
    <name>A0A8S1NGL7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0800114</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGL7"/>
    <dbReference type="OMA" id="RIPVLNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001037">
        <property type="entry name" value="SRSF protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF9">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="91"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="314"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="314"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8088155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="649" mass="76586" checksum="0C6FAA845766A099" modified="2022-10-12" version="1">MYTSDLFQSTVMSKSYYQVLQDSNLIESQVLQPVCQMATSQYMTQSMQKPKKMELFHQLDEEHDSEDEGIEDYKIGGYHPVHVGEVLQNRYVVVQKLGWGHFSTVWLCKDFKFDTYVAIKIQKSAENYLEAAYDEVEILQKVAQHVGDEKWLKKLIQYKPNQRLNRDDTHVVQLLNSFVYRGPYGCHFCMVFEILGVNLLEIIKRYEFKGVPMRLCRKIAKEVLIGLEFLHDHCGVIHTDLKPENVLLQLTQDEIRDIIENGQLTKNQIFKDRLDFYHQLFDIKKEEPIKLEDSVKTVINEELKVNEQVQENEQQKLTKNQLRNQQRRQKKKQQKQQKLQEIDMRIVEEEQFIKNKEEEIKMEEKREFQTNLFKIDNKSLFKQIQKNEFSVKVADLGNACWTHHQFSTLIQTRQYRSPEVLIGSRYNATADIWSFACMLFELLTGDFLFEPRKGANFSKNDDHLAQIQELIGKFPLKFSQRGLKSKRYFNKDGNLQRIPVLNCWSLTDVLIEKYKYNPKDAKELASFLKPMLNPYPERRATAAQSLQHSWLKSDSDGVKMNDEELREYKISRGQVEFKKTPESEDEYADRSETPQLILPLPSRHRYRIEPRCVNRNEIDRSFTDLGYIGFGNGIEIDMLDSTGNWQFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGS5</accession>
    <name>A0A8S1NGS5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0890126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGS5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8091918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="336" mass="39340" checksum="80F492FB1748169F" modified="2022-10-12" version="1">MQGEELLPDHINDEIYRKLEHLYLRLNEIVRKAEDVLDLSQSVSFQATLKRLNTASKGENYQKIPKNYFPPPSDQESFERKFKLLRRCGHILSDTMLKVKTSLVELHDATCKQKQDIVKMKKDEYTIKHEKPFSINQQQKNGTQQLKPPQTILLDEDDDIMILPKIEYVIEQPQMLSISIASDPVYYISCTCLLQNTLQQQIPEEFKLCIAIESLQYYDKFYLNSNIIQECKTANRILLQLNGDSNCVTKNCIFYEKVYLNMIKQPIIQLNFAKYKQVSNTISLSSLILQIYSINKHCFYMGSINLVDQQGELPFKRLEILSIQLIYYIFNLLQVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJA7</accession>
    <name>A0A8S1NJA7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0860089</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJA7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF33">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3 4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="6">
        <location>
            <begin position="6"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="353"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="438"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="83"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8090546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="586" mass="68672" checksum="EE2F2052D80C61B9" modified="2022-10-12" version="1">MQNQQKIRVAVRKFQYKNRLEWIGNNNKRREKQKKLQVTCKSSRFDHVLPETASQDQVYKSCEIDELVQSVIDGYHVTVFAYGQTGSGKTYTMEGQRDNEGIAIKHDGIIPKTIHSLFNKIKQNALQRDYSVYCSYLQIYNEKIYDLLGETSKANYSIPINGLKMRWNVRDQFLVENLFVYQCKSADDVIKLFKLGSRNRITASHKLNFSSSRSHSIFEIKIESIDLKNPDYFITSKLELVDLAGSERISITGTEGKQAKESIEINKSLMTLRQVIAILSDTNNKTIPPYRDSKLTCLLKQSIGGNCFCLMIACIAPLDIFYDENLSTLQYATKAAYITNIPIKNDDPKLKVINELKQQLNSLKLELSKANEQIEFLSNIVQKKDNNQNINNINTKSNFNKLQKNQQRQLEQESSEDDVNKFEQAQLNDRLIDSINMVRELLLSNKEMREREEQINQKCESMYREIQFLQKDNFELRERLGDPDQSMESHQKRPQTIRKNTMIVEEIGCLPEQMTQFQKNFQKDYRDIQNKDPQMLMRNQNIRAYSQRTQSRQLPLISNYQLSFEEVQQDEMTNFRRRSLYQKQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKT0</accession>
    <name>A0A8S1NKT0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mini antigen</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0910090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKT0"/>
    <dbReference type="OMA" id="KTEQCEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035848681" description="Mini antigen" evidence="1">
        <location>
            <begin position="17"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="34594" checksum="20B1BEC0C4B5D381" modified="2022-10-12" version="1">MLHIFVLFNIISFLDAQTCSTTQITCESITDSTLCYETKDSNINQICEWNTNKCEKAILNNLPCSKYTNEYTCFYRSYGCRWDGTNSTGYDTEEMKNNVNGKCVDQKCQDFTSEHDCSSFGQISCDWQDDSCVQVTKCQDFQTVKGCRNTKFKEKCVPIVNGEVLSLGQKSTTIFDSFECVVQQCKHKSNEYECTFVNGVQCIWGTTGCAVCASFNTYEACVQNKGLCLWNQNQCQNIECQQYKSPNLCKLKTEQCEWNRLKMRCQLNSTQANQHCYSEYLDQQNQGLVIQIILIISFIFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NNN6</accession>
    <name>A0A8S1NNN6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0970087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NNN6"/>
    <dbReference type="OMA" id="YFINLMS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="12605" checksum="89BCBD3900561DD6" modified="2022-10-12" version="1">MEIQFVDEKFEYFIQDQLYENLRVEDDPSKVKEYFILLMSDLIKDKEVKIEPLNDQKEYVAQVGNGDCYLIKQSRIFKCNSFSALKSKYLQNCSTALSNMIDLTLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NSJ0</accession>
    <name>A0A8S1NSJ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0850019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8089944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NSJ0"/>
    <dbReference type="OMA" id="TACEMNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="194" mass="23300" checksum="BEA46053EE31D429" modified="2022-10-12" version="1">MILENPSKIKWKQQYIRKYRDLLGISNLKVLPPEPTENHITPQHLDYINKQRSKSLKQNDESILQCSDRRSLHTKIYTACEMNQIINHAKICGLLHDKQSQKSQNKQNQSLLEQNRKYFQDVRNLENSWNYKRRQQEEQNKLFNSIPMEQHYKMKLKQKKIDLNIQQLIEEQRTLFLTSNTQQNNELSSSSYPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NT19</accession>
    <name>A0A8S1NT19_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0980144</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NT19"/>
    <dbReference type="OMA" id="HENQFHR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="33"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="564" mass="65831" checksum="60B60B2947D67FBD" modified="2022-10-12" version="1">MGTCQYNNCKSYIDISTLNTTVNIIDQLNNDFMVVHEDYEHPLYGQVNILEYKEPKQYDFKLVHKLITFTEQEDFQYALQYYQAKFYGFNHPNLLIIHAMQYKQIDQFFTTEYKLSLFLDYYETTLAQELIYRKKIYFEETEILFFLDSLIGSQAFLQSKGYALPSLKFDKVYLSNLLNGAIGLKVQSPLFHNQQDVEFNELFHKIINGEEVRLHEYPYITPEQWLMIQNKQLEPYNEFKSNVFILGLMTLELCSAKQSITLYQDYMIDPNILIEQINKVNDRYGEALPLILEAMLHYDPKDRRDFLQLNELLDSSQLKGKRLAQIWTSDDYQYSLTEKKVHFSTKQHSLVHPEVQSQLSNQSLLQKGSHLSIRQVISKNSKESKASSSFIQKKESYSPQIQKKNLFKKFETEQASKGGSKKLITQDGFGVETTPNGLTYEGFFNAGKKQGMGKLINDENEIIYEGYFHENQFHRTGILKNQNAQICQEAFNYKDFNQLGEKWARYDGDFNFGQQDGYGQLVLTNAERYTGLFKNGKVHGAGVFQTLDGKQIKGVWINNILQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NV09</accession>
    <name>A0A8S1NV09_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0980058</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NV09"/>
    <dbReference type="OMA" id="CRCDNIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06612">
        <property type="entry name" value="STKc_MST1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000421">
        <property type="entry name" value="Serine/threonine-protein kinase PAK 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF2">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="12"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8095209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="525" mass="60942" checksum="2C7829542F3FBFFF" modified="2022-10-12" version="1">MNETQINPENYYRLECVIGEGSYGQVYKGIQLDNGKVVAIKIVPSSGEIESLKREIQILRDCRCDNIVKYFGSYHSNGQLWLIMEYCQGGSVIELIKSLQNPLPEEIIATILYQTLKGIDYMHSHKKIHRDIKCGNILIDHLGNIKLADFGVSTQLVHTMADTDTVIGSPFWMSPEILLKSRYSKKTDIWSLGITAIEMAEGEPPYAHIHPIRAMFAIKNNPPNSLTDQSKWSKEFNQFVKRCLILDPKERPSTKDLLQDPFFQKYCRSREYIQQFILKYKKQIESYKLSKQQKQHEQQFQFSQEQVGVPLDTLIECEEEEDLGTMIINNVDAIQMNETGTMLEHQYEDADIYEKAMNRVSEYGLSSLNQQRTNTIISESPILKKKQIEDKINQLNQEMQNEIKQVKLKYGEQINSLQKALNQIVVQKHETHKSVHEQKGFAISTNYFKQLEQKNKFSAPSTPVQSQSKYRLDQENLNLENQLKQTKYQSTNLQKLQLQIQNRINDVPKLDNSQKLIKQLINRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NY12</accession>
    <name>A0A8S1NY12_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0980094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NY12"/>
    <dbReference type="OMA" id="VQDGRYC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039341">
        <property type="entry name" value="CFAP99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34649">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34649:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="569"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="719"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="378"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="719"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="768"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="779"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="788" mass="94572" checksum="84EB6AC27A999228" modified="2022-10-12" version="1">MYELYMKILKQYQILMRYQQLITHAIDLIKSYNPIILTPDSHCEQYIQENCKKLFDTEIMFLKQVFSGVQRYEEFLKILTKTMFTILSSTTNRNDGTLYQIFSYLIIFRLDELPYNEFKKMVNEQDPVKMNVLLQFLFDIEKIQTHIKPLWIEIYDPQYIQETVIGGLEQHFPMMKDLLTSLSSRATGKQSELVEEDERPQSATKKQPTKTIPFKLSETKKKPEPPPPPKPEQYKSKPVPSYLNKRTLSQVEEENKQRLEQSKKKVQDTYKEAKQFEFKTEQRPTNYETVKKEVEDTLNKQLQFNMKYAKPVPTELEAAEIKLNAAAILREEMLLKKKQEIEKERVKNLEVNLRDSGEFEEWKKQQDEIEQIARMEHQQQKKIEMELAREAAMRAQEEKFKENRILAEKMKEEAIERLKERQELQQEQVEHKKQLIEQIIEAEKKVHIQVEKVQEKNKQMAEDQKLEMARKLELKRQEDEQIRIKREELIKQIRELERQPIKRTKGYDPTETMGYGLLEEMSLAELRDRLEVVKAERKAEEEEKRKEIVQSKDEYLSSMQKKVQEIKAHRRAESQQKNMEREAKKLKKQKEEELRKKIREEQLIEVQSKISQKKQIKSAEEQRIAAEVRETKLRQQYMNANKAMVEMKAWKSQQDGLEREIKNRQNEKLIVQEGIESVNLKERKILAEQAKRLVKTKVDFNQAYDKDLRTAYQMNEELHREEAENRTQMHNIQREWKEEHTKNMITRDEYKQKISETSLQNSKKKSQQQKVKFQQSRQLEQLPSAQYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYF8</accession>
    <name>A0A8S1NYF8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0970035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="RIMISGV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035790685" evidence="1">
        <location>
            <begin position="17"/>
            <end position="2059"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2059" mass="230659" checksum="8FEB8691432157DF" modified="2022-10-12" version="1">MLILLTSLISIIKCGSILRPTGVTPIWNSVANADVQAQYIFSYVLETYLASYSQIEVQFPLQYLSGLGTTQSELVCIPSCVVSGRSVIFTYSEEMKNGTIYTASVQPIRNPATQGGTANFILRSKRGDNVIDENLIFGIIGIADAINTMVFGEVVVNPTGSFYAGDYTQYILKFKTTTTIIYNSYMLFTVPTDFELEPFPVCSAYPINGIQLDGTLFCSQVNQTNVVKIEGITGQVIGLQDYALKISMRNPKYAGTTKTFNLGIYKANTQMMFEEVKGISGVTIQAAQLQNVSMTQVQNFINSRSRIMDYEVRFTPTFPIPAGCNIKLIFPVYMQIVQPGDYNQAGQFQMNYVKYGLEDISEDSPIGFFYQDSIQNYLRITNFQEMTMNNEICVVVRVINPSVEGYTTPLKLYVFQDITEYQLLQSDVLYAIADVATVSAGNQNVPTTNTNNWFLKPRTGFFPNYQDYSITNNYANGALATLTLNIDPSATVPKFAFVYITVPNEFKVNNDTITGVNAANCKFYLQQNSPTLNGFVSPTNSLSCTATDQVIKAKLPFAFDVIQYPSQALPAAPLRNQWQIQLTNAFYTPSLNGTYFFDVTIKDTDDLTVLDSFSYPVQINANLLTGSIVNSIPKNSKEKAIVDIQFITVLQIPSGQKQNRASDTRGFLEFHFSNAWAQDLGSGVSNGGSIPCYGVNALIPYEGSELKCILFHSTATSKTIVRVSNFRYISTNTNVRIMISGVTNPTAASYDIDIKVFQKKNRIFTLLNQQLTVTSTLNAAPATITCTTAPSYSENRVGYLFDISISPCLTTAISANFYILIQLPKYDIGFIRDQNTITCSLTSQLYCIPLLGSDFILIVTSASVPLSPTPVFKISNLQWPRYVEDFSTYTLNFLIINNVGLGVVQKYQFNTLTNCVPSQFSAIQSLVSNKGKGFVDATYSFTFTPQADIPEGSTIIVEFPPFYDFLNGIHLPTFQQSGLLDLSDTNKVSIQINLKLIKITGFKQFNKSNQFQVIAVSVKNSQSYNTASGFHVYVLFNGKYVLQQLNFYSFDFTTPFKAGKIVINQITASILNADEEVDYLIQFTPQNDIPIGGQIKVMFPDENYKKLPSIPDCRVSGGINTFQSCILNGKTFIIETNSRYKAGNGVIDLVIKGVQNPDQGTTQGFVISTEYDGVKLDGTDESNLNGRTFTAASKTNPIILNSLVFDPKNEGEEASYRFIFLPYSQLSISTQIEFKFPNQFDPLLGRDIQCIVNSGLSGGSYTCSVDSRIVTLKGYDAYTPEISNPIDITIFGIVNPNSNGGSTTDFLKLYIRQQGSNIYDQSNKEIAQLSFLAAPGWSPLISIDVSTYNVRWNSTYNFTFQTYKNIPKVQSKGSVLIDYPSQFEIIDGSITCSSTTLFAATLKCSSYRNRITSSGNYEDYVGRMYIQLKKVLNPIEKGESDFFYIRTYDGLNLRIIERNFANLDPFKFSYEFPGPLIKVNNEKTVTIERGTQAINIPITIEFPCLLNLKLIPSENEFIVNPSSIPLTLGMTLQHFNISVPQSIYDGTYTIYWTLDNELDPPLYTPVKRTLIQITKKQGVKITQTPILSIPIQGQSLLTEYILAAPPDTELTFSLYLQRTYFAIQLSKTAITVAAGQQKFGFAVEYNKSVNVDGPQVERGYITIQTEGVNKNIYKLPSTSVEFFIISQDTKPPSWVTCKIDNIKKTSVNLLVALNEPCVVYYLIALKGTVFPSLGDFQNQIIRYSSTQSQMGYLIAYTADLQYQIPLSKLIAETEYVIHILALDRGNNQAEKRSIEFKTLDRPVSCYFTLIFNQRTVSDDFIRNVTESVAKILGIDQYYIQLQQYYFNRLLQNSFGITVDEIKSYIDLELAAIPDSDSFPDPYDVVAGLQNKTQLLTSRIETLNLSKEVTAQKFTRYVPRFIKQPKLVYIDYQQCAIQLAISNYGWGFAVAVVFESQDQTWPSPYQIMKGLDYQNRPTPNGQIEISDPYQDFILRIHGLESETDYYIYVIGGSVHFRAPDLMDKSEIYQDRIISFRSPQKPKLNINFGDLIMMSILIFWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1Q5</accession>
    <name>A0A8S1P1Q5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0960078</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Microtubule inner protein component of sperm flagellar doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RIB43A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1Q5"/>
    <dbReference type="OMA" id="KLAXSER"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008805">
        <property type="entry name" value="RIB43A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14517:SF6">
        <property type="entry name" value="RE41410P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14517">
        <property type="entry name" value="RIB43A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05914">
        <property type="entry name" value="RIB43A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="163"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="218"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="44"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8094554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="243" mass="29516" checksum="C910E5384051A0EC" modified="2022-10-12" version="1">MSHYQNPTYNHAQMKNQVGVSNLKMLDGEDLTAGDRRKLQQLQMKDWVQQQTQENQQKKQLNKQIQQQYDQQTLQINQSLKELEQEQQRRRVEMEIANQQINNQLAKEKQDREEYMARQAQLEKKQHMEEIMNNDVWTENTATCQSALAPHRVIPYHYKGMSEQQRQEIRNDQAKQREQNEQKRQQEKEDEKMWAQYNEYNRKQLIIQEREKARKLQTLRNNQKESNLLSQTEQKLKLKNEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P2N3</accession>
    <name>A0A8S1P2N3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1020035</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P2N3"/>
    <dbReference type="OMA" id="NCTSVEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000161">
        <property type="entry name" value="Probable dual specificity protein phosphatase DDB_G0269404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF511">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="42"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8096654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="199" mass="23112" checksum="9DFFE786CA139A59" modified="2022-10-12" version="1">MQQGEETQGKKDNENTHNIQVQNLMDKIKILLIMRQLKEDSIPIEIIPNLYLGCLGCALNKKKLLEQNIKFILSACEMPKAPFSQDFTSLIININDSVDQDIKSKFEESNQFIENALNSQQNILVHWYLCQIYSFAGKSRSTTFIIAYLIKNHKMTVNDALELVKTKRQIAQPNTGFMKQLQQYYDTLYNNNNDQKQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P2V0</accession>
    <name>A0A8S1P2V0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Matrin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1030173</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3A3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P2V0"/>
    <dbReference type="OMA" id="QRCYHEE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051421">
        <property type="entry name" value="RNA_Proc_DNA_Dmg_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031774">
        <property type="entry name" value="SF3A3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024598">
        <property type="entry name" value="SF3a60/Prp9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021966">
        <property type="entry name" value="SF3a60_bindingd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786:SF2">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786">
        <property type="entry name" value="SPLICING FACTOR SF3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16837">
        <property type="entry name" value="SF3A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12108">
        <property type="entry name" value="SF3a60_bindingd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11931">
        <property type="entry name" value="SF3a60_Prp9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="9">
        <location>
            <begin position="405"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="342"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8097317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="500" mass="59702" checksum="8AEF9BB5CA32AB05" modified="2022-10-12" version="1">MSSTYLEQVRAAFSQIEVIEKTIVFCMQSKQVIPNQSVLLDHRIHNLIQMAQKTAADALILIDGADGWKKEEMNYLQGVGGSGDVWENFYERFKEIKDYHKRVTNVQTMQNKVEFYYEQAFAPPFKEPYFSGEEHHGRFLDMHASYKEFLNLKKLKESNKIKIGDYLWYLQNFQNFHDIPLYIKEKEGSKYKRYLINMLEYMRSFYQRVNPLQDVSKIEQKIDIDFQTQWENGQVKGWEHRKEETEEIRGHPHYCEACQKKFQNENTFVNHFDGKKHKALAKLKEKQIEQVIQSINQEEQPKEDNEGQKKKKMAYLEVAILQYKDTLQQQLNDTMNLVRKKQSRRYEENEDDEAVPVQDQQLDQPEEASSEDESPIYNPKNLPLGWDGRPIPYWLYKLHGLGVEYKCEICGNTSYWGRKAFEDHFQGWRHSYGMRCLRIPNTLHFKEITKIQDAISLYKKIQQDQERSKFRPEYEEEYEDTDGNLLNKKTYYDLKKQGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P652</accession>
    <name>A0A8S1P652_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1070073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P652"/>
    <dbReference type="OMA" id="HKIQDEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="248" mass="29684" checksum="9D9C00BB9B296275" modified="2022-10-12" version="1">MKQQQISTPSTQFGLRHSLQIQSSPNIKLYQQSTYHSPYQSPLLQQQSLSQQRQQIQQNNLVNINRSLFNDNQNKKCKQQQIVDLNNIEEPWRQKVFELQRKIQQLEQQQQNQECEQDESELSQVYSQIQQLVNTIKILQEEIQNLQEKLNIKQNIIDGYDKQLIGKDKELDDQNHYIQELHNQLEEQTLKMDDKQKLLLQEINSWKKKFIEQNKGLHEIQEEQIKIQTQIDNLKNIKLILNKQSEQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7M6</accession>
    <name>A0A8S1P7M6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1080141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8099046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7M6"/>
    <dbReference type="OMA" id="CYPMMTP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8099046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="146" mass="17560" checksum="5895068C88AB911C" modified="2022-10-12" version="1">MSFPNQDYLAMIKSKQQQQQTQSFQQQFCYPMMTPQPWNYWMMQQQFQQMPQMQMSPMLPQQQMMSPVSPFNVSMIPPQFLLQPQQTQSKLSTSQSQPLQNKQYVEVNGTKVKMYMKLIKEDDDMLDYDEIKREKQKKVNLTNKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PAL3</accession>
    <name>A0A8S1PAL3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1110178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8100196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PAL3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="98" mass="11404" checksum="8B1D5B043C8D8446" modified="2022-10-12" version="1">MKFQNALIVSSFCQDLTSVNNYWINFAIHRLNSNDLGRLITINSMVIVNALFYQLNVGLIIFFKDKEKLISNLSRHFMIHIVVLLWAIFVNGIINYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PB71</accession>
    <name>A0A8S1PB71_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1110073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8099988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PB71"/>
    <dbReference type="OMA" id="WIKQPLD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053128">
        <property type="entry name" value="Cystatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12319:SF2">
        <property type="entry name" value="CYSTATIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12319">
        <property type="entry name" value="CYSTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035927174" description="Cystatin domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="16199" checksum="5B1BF465737FB3D1" modified="2022-10-12" version="1">MKAILLIALILCFANCSLETIGLRHKLSQIIQEHQSSLQEKQMSGGWIKQPLDEFEQQNNNIIKSTQDAIEDKFNLSQDGYQYEKLLQVTSQVVAGINYKVLVQYEKEDAKRIFEVQQFVVPWNPSANSITKVEEIISQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCR0</accession>
    <name>A0A8S1PCR0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1120158</name>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1120160</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCR0"/>
    <dbReference type="OMA" id="CVYFGNG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001534">
        <property type="term" value="C:radial spoke"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22963">
        <property type="entry name" value="DD_CrRSP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006802">
        <property type="entry name" value="Radial_spoke"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13159:SF0">
        <property type="entry name" value="RADIAL SPOKE HEAD 6 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13159">
        <property type="entry name" value="RADIAL SPOKEHEAD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04712">
        <property type="entry name" value="Radial_spoke"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="413"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8100523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8100527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="443" mass="51015" checksum="9EB8E9B0190965F7" modified="2022-10-12" version="1">MLKEKLQEIKDSNTNLYQHLNNVLTKILLDNPKNAFELFEDYSFQVKSNGFTFDKYNEFDNAERTKVTMDQAIQQKTLKLINKINLGTDEEPQESTQTGYLPNLTEESYLYEWAGIGIDENYKIFKALTLLSVTKQASKLRFWGKIMGRDKDYYIAEGVATSTVEDGELPPEVEPRGSGINTKSYWACTDILNDKDWVELPLVTPQQINISRQIKYMFTGNLENDVITNPYFFGKEKHLLKAQIVRITQSTVIIPKGQYILREGEPREIDPAPEYKLPGFHDLKTLNDWMHFNQNILNCGRLVHLIPSELPEGVEPEAYQKQIEAADPFENRLKPISNDKIQNNKPAWKIRIVGDTTDYNPLAKDTNNKVNNGVIIIKSLVWPGLVTVYSNKVLSQLYYGYGFKATNNHFYPKQPQQVQQEQPDLPEQPEPNFPAEVPQQQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIZ7</accession>
    <name>A0A8S1PIZ7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1180095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIZ7"/>
    <dbReference type="OMA" id="SINDQCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="19"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17446" checksum="63860B76BE6AF78D" modified="2022-10-12" version="1">MQAIEEIKRIVKQDSFIMGQLYHAVGEIHYFNHDFEDAIEYFDAAYDIKIQYPKERLSQILTINYLGSSCYHLGEYKKAQELYEISLSQITEKSTLIEAQILNNLAMTKIAQNTNAKNDLDRAISIYLIYFSETHPSVRRALRNLKFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLN5</accession>
    <name>A0A8S1PLN5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1210053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLN5"/>
    <dbReference type="OMA" id="HYKAISN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="88" mass="10500" checksum="D35701B578C772F7" modified="2022-10-12" version="1">MSLTYLPFHNLVHCPKQVIEFVQNPDLNLNDLNNMIEQLKMPKNHYKAISNMQYLASKQEHYLQDDNTLQNQDEEIHSPKFIFRKILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PPU2</accession>
    <name>A0A8S1PPU2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">IBB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1260033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PPU2"/>
    <dbReference type="OMA" id="IDKVQME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IBB" evidence="1">
        <location>
            <begin position="5"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="526" mass="62792" checksum="023CE6A871C36C44" modified="2022-10-12" version="1">MNSHLQMKREQFMVQIRKQAREEIFSKKRHTSIILDGDMLQKCNPDETINFIYQTYLEQDFKHLAKLLKQYNMNYLKLLEDDNINDQVVLNQFINHFSSANLNGLKLFFDIIRMSDIPIEFNLTSDRLMCINHVLVILINITYLDRKDIVENLLQNDLINILLKNLIDRMVQPDKIQIHFDRWCEILESICLILINIQFELDNRNVLLFREEILKSQLFRVWSKFYPKNPITKTWKAILLLECKLFESNEIDLIDIPHYNFNCIISQCTFLIDKVQMEDESLYKRVLEILQKLSEFKITIALTLTLTKFVWEKILKLNLFPSKFFSIFTNFLAEKDDDHYILQYLIDIGLIESMVNSLQNQGDEVIIEKIFKCFNNILYYPHAFIVERTITVFIPFLHNYLNQYSIVKQEICIEYLYLLQNLINHQQLSDYHLNEIFINLDCLQYISDMLLSCIYLTKLIAELFTQFQKLPPQFKLVILQKVDTYDFVEILNNQATILKNKDDGSINLILNLIQLWEQSDFEFNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PVZ0</accession>
    <name>A0A8S1PVZ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1330070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PVZ0"/>
    <dbReference type="OMA" id="DFMHTHP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="140"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="638" mass="76018" checksum="03B3E709EE485818" modified="2022-10-12" version="1">MNELENEFLTQCNIESSAFSHSYIEQIYNEVHFNNIAKRDQIARLKKEKIQYQIKQEKQQLHVEEIEKALEQKQYLEIDLDRILYSILLRQKYINDLEDQLRLINSFYQDLPIEHRESQQTKKLSYLSYSKYKLSYDEYIKQLENKKVEVQDNLEKIKNETQQTQQNYYSLLQKIKQAQQTLVEQEKTIQQLNDQNETNIFKLHEQRKVVIDLQVLQKLERMYLTYEEGINEEFITEDINKVDCNKEISDFMHTHPFGVSFLVENLIQQYRKYQVQIDSESSIGLGLMEEKQELQQELKELKELCERFYIYDENQDEDQQFQDIQKLMGFLTMKQSQNNFKQNLLHKAETFIIKVFGNLYNAIKRFNDIFQAIHQKRMAIDLKSSQIDSKIKNFCANTYIGHYSLSLYQYMNNPLTSTQPDLYYQIQNSIILKLYVNEDALNQYLQAGSTFIKLYLKASDYYSNQIHSMIELIHSITQIPKRSKFTYNDSPLLQQNYKSKLSIELSQKKISLRTDIESRAQVQVNFKTKTTKRNSFTSTLGSSRRIQQDINEENYLESERQKIINPKNNISNTTKIPSEQQKYCDLLSERGHGFEKKVIIQNFKSLIKLRQIKRKFKTNPITPKSIIGSSLIFKSTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXR5</accession>
    <name>A0A8S1PXR5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Calpain family cysteine protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1350043</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXR5"/>
    <dbReference type="OMA" id="QYDYSEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000261">
        <property type="entry name" value="calpain-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF379">
        <property type="entry name" value="CALPAIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="101"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Calpain catalytic" evidence="7">
        <location>
            <begin position="237"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8107852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="777" mass="91328" checksum="CE3A405C013D3603" modified="2022-10-12" version="1">MSAVEEIERCLAQAIQQRTATEIIATMGTKVLTLQNFQQLIKKNNKNIINKEVEEYYYKILTDFDSRVGITQEIFEKMVENLPKPKRRQDVVMSLIEGIQSKKASLERIYKFFDYDGDGKMQLNEIKELLEYLYPEINEEDIKILMKNNNEVQFSFENIKTLLSFEQNMNAEIVIVKDENDISDSEDDDEYYDEYGMFKGNQEEYFDEYCFTEDSPFYQDFMNFKNLYFKLKESKTLFIDPDFKPDETSLGNVDEKFEWKKLTEIWKNAQIFQKDLYHSNNELGVHTLVSPRDIKQGLLGDCYFLSSLGSIASKYPDKIFDLFQTPIINSYGVYGVWLCIQGIWKLITLDDYVPIYNDQPAFSGSDSQEMWVILLEKAWAKIFGSYGNIISGDPREVIASITGGPTWCISSDESTFIERLKQIMNSYQNYIVCAGTYSDKPEYEKLGLVRNHAYSVLNFRTIKLPSKEEVQLIQLKNPYGNDQEWNGDWSDKSPLWTQELKDNVLQNQEEDGIFFMCIEDFRKHFENVFIGFCNPYFKFFQITIQCQKKKSAYILLNIKKDGDYYFSAYQKSQRMFKQQSKDLQQQYDYSEMRLILSRMNKNNSIQPLTYKTETDMSFNIYGRLQKGQYILQLKVNWIMEGWDQSDVPLVSYGEDTTEMKFCQKISNFQQASILFEAKEKRAEAKPLNPSHPELVVLKKKSQAYGYPYLYYQNLSKEKTFNFEILFEGDIKLKKPHSGNQVEIELKPGQDKLIVYKFMVKGAKMKYQIHKFKCGSVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1B6</accession>
    <name>A0A8S1Q1B6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1370149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1B6"/>
    <dbReference type="OMA" id="QFFEISE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="94" mass="11405" checksum="4672976D3FDC1B88" modified="2022-10-12" version="1">MSKKLKEFLNLDYQIHNKCLSYQAIRIKTDHPKMDFIYLIWKMGLQRNNYLWSLESLDKLAMLNYTDINSLGNQSIMHSYIFHHKKKQGKQKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2S5</accession>
    <name>A0A8S1Q2S5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1410031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2S5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="216" mass="26625" checksum="671DFD8D9D0A747F" modified="2022-10-12" version="1">MFKNLMYVEYGQELQCHEKLYQNALKKQQKQKEIDYQKQLQMLINQVNEIEDEPPQQITKDFYDYSMKWKNLVNQKRFQEQYQNEMSMQQKSQQHQIQKLPQNYLSPIDGWKYHAQQYFDKKESRQQNESSFQPSINEKSKMMEFDEPVEDRLIKYGQQREQKYLFEKQFEKFYEQVQNDQRPTKPNEEVFLRLQNNGTYNVISKNVNNHNLKYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5J6</accession>
    <name>A0A8S1Q5J6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1460009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8110926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5J6"/>
    <dbReference type="OMA" id="HNIHEMA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="237" mass="27818" checksum="604B1BAE112CD037" modified="2022-10-12" version="1">MNSTTPKSSGINYAKLRKQRSNQDTLPKSMSTNIKMDINQTQQFGGIKHRSTSQHNIHEMAQIQLFDQCLVKQANQNEFLVFLDRQMQYYNQTTPQNFSSFRVNNKTIQEEKKKLQIELQKFNQENAFLIKEIKDLNKTKQMLVKRYDALQGLILKERLNNQPRVQQYSNPKFQHLFNQISPVQETISSLTDERFESNATSLDSSLSYEDDQSSASQLLDLLQKNSKHRFNFQKQMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q940</accession>
    <name>A0A8S1Q940_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1500093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q940"/>
    <dbReference type="OMA" id="FCICGAK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="484" mass="56634" checksum="B846D4DD95385DE7" modified="2022-10-12" version="1">MNESDLLRCKREQFHTEIRRNHLEKIFNVKRKVSNSDAQISIYYTQQMQHIGKIKILDLLILINEKVNKPIVEMVQNDLGLQLTKLLNINELYSNWELFKNILIYLNNLVLLPDEHLQSYPKFNEMLISFSQLFILLEAEIFRKGLCIKGNDQNCPLETGYDDISRLSYAFIKIDQILMYSSTTFQSRGELLRSKIFVHSICHAFHNQMDNSVLELHLQLITSLLQDLYKQDNDCRKHAEVFLKPICSALSTKNLDKLNYLLQILLNLTFVGMHNGYINQIFKFNGGLELMQILNEGLNDQLTLRILAEIAQQSPAHIEILIKNGLIQQLKQKLCSNNKQVQNQALWVLEFCICGAKQFYDIIFTQIDITNYVIQLLNSPDEQIHIQACFTLCAYLQKGNFQQTQQLCQSKIHLKVFQFLHLQNIELKQAIIEAIIEILNKETWQSSQYQLISPQERENLRFVLERIDEDQSIFSMINNLLSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBA9</accession>
    <name>A0A8S1QBA9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1500097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBA9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="175" mass="21192" checksum="A4FCDA521C013AC2" modified="2022-10-12" version="1">MERKILQRCLSFNVQLKIQFEFKADKYYILIEVYGQQYYFKRLLKHQEEIMFNQNELELESVLENLHKMGQVLTYDLCIVPVKFEQFAEQVYYGREGSYNAYYGHIGDGDINYNIVLENVEEMHMEYIKSRREDHYTYKEFEWMYMCGEYGSRLIEMILHASLKGTMKLLSICRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QED3</accession>
    <name>A0A8S1QED3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1560071</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1560072</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QED3"/>
    <dbReference type="OMA" id="DQIKVHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036434454" evidence="2">
        <location>
            <begin position="17"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="61"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8113716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8113718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="319" mass="36500" checksum="CC501F049ACF861B" modified="2022-10-12" version="1">MNRIGFGLLCIMLVTASSNVMLQTADHSAFIEVFKTYSKQSEFGGRLFGELLMMVQKGETLQSLNDHVEELRKHLADDESEDEKYFNEKQQEMEATFSGLANEVEELRVSVEQAASRIQVLNNQQTIIDGLFANDQQAYQKRVGDQNAILDVIGQLLLRVSELQQGGGHSLIEKDNLLDEIKALGRGKHIEVLAQITAHLQTEQVEHIQELLNRLRDAVLNSLAIDEEKEQENIRLYNKLSGEIRSSLAQSDRLYQELDAHFQQKELELNTKKLAVREVGQTLQDRNDQRQFILDRVQEAQKLLNDNLDRIARWSVESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QRV6</accession>
    <name>A0A8S1QRV6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1950004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000204">
        <property type="protein sequence ID" value="CAD8117307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QRV6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035848122" evidence="1">
        <location>
            <begin position="16"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="189" mass="21550" checksum="A63E5F5193598139" modified="2022-10-12" version="1">MKFLLLFFLLGFSDFSTLPASDANAKFSQNDLYVLREKSIIRGHYKDVASQFNGALLSSARTISFWESGIDNSPLRNLLYYTPNYLVIGHVVAQLSKSIIVQFLQAYEINTVRFWMWDGDTRQTDIQVFTIAKDGQTENIIFDGFTQQNIMVVKFSDQLVSGLKFYNRGGNTKGTQSMSIIKIQAFYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QVC3</accession>
    <name>A0A8S1QVC3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2540006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000263">
        <property type="protein sequence ID" value="CAD8118647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QVC3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8118647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="60" mass="7535" checksum="59518DDF0D7C0991" modified="2022-10-12" version="1">MRDQNLLGNKQKNNRFFRLISGNKIKQRKRRDWVWIFSLLMSNLIWIMKIKEKRIFLNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JLQ1</accession>
    <name>A0A8S1JLQ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JLQ1"/>
    <dbReference type="OMA" id="INVQWEE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001230">
        <property type="entry name" value="CCR4-NOT core complex subunit Not4, putative (AFU_orthologue AFUA_6G08820)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039780">
        <property type="entry name" value="Mot2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="38357" checksum="4D1622515FBE5BE6" modified="2022-10-12" version="1">MKNQTNQSEICQYCNLSDCECSDPEDWDSCESPKPKTLYSFKEINLQNKSLLAEIRIICKNLIYVIGLAPNIAKEDQLKKLEYFGQYGQIQKLIVIQSNTFNPPSHAAYITYRNEQEASLAILVKIINIYLFWHVKNFPYTTDKQRLPLEPQNIVQTSQRVSNAKSRIVYTCINILKTKTLHKCQKKYEEMNNSKWLFSYSQKLAQANFKKFYTKINYKNALQKSVFPTTQNILDNMIKERVVEPIQQQQQQINVQWEEIKETQQQQELPQTIQQQKPIDIEKRVEAVILKMDGDSNSNSRFKFTNSKNVPQDYEAIQQIKQYLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMH1</accession>
    <name>A0A8S1JMH1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0050165</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NNCINCA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035872960" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="3576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3331"/>
            <end position="3351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3431"/>
            <end position="3455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3461"/>
            <end position="3477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3498"/>
            <end position="3521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3527"/>
            <end position="3549"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="3025"/>
            <end position="3036"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8043512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3576" mass="415980" checksum="27C5FDF4CCF301B4" modified="2022-10-12" version="1">MINLISITLLFWLAATQYKLILQGPASKKMDCKKLDKNDNGFKGSQFFYEKLSNYGQNTNLDKFTFRLWFMVYQKMRFPGKQILFAFLDGYDTDSYINLMVYFNLQSSNYTLYVINEKMNSELMNILIDQQNDFFIGYWNYLLISIDQTSGDVFLNLKLFSTYNYQLQQFQDSLPSKKLNFRFGVHSRITNEQLYQIIQDYKACVYIANFEYINGWTTMDREIYFQDQIDLKYSLKPFQSEGLNVNSQFINVKLIDQKNIEYSGNIGLNIYKDTRILYNFEEQMDSFTIIFWMKPQKISSFFKLISLTDEYVKLISLGFGVNQNYNFLIYQNKLEIYTGNLNPNQWKHITIGILEISRNQYFKATNSVKMLKVYIDDLMVYLQQILNFKSYKRLLIGPLLLESRGQEVVDIQDFKIFNGYGIYESSGDCRLFAGLYCAFCKSLTHYCKEQDPLDDDNIYQCPAGYRIENGYCKPNTIALCLRQIGGSCIECEKNTILIQGECQPQTQLNRVSPYPCQGLNVVLCQIINIVKNSELTSVQRARLCNSNQYNIAKFECTGNQVASCKYSQYSGLCFECKESFYLTELKMCVSTCFKNKQDRFNYINKCVQDCPYKYTFNHPCPTSGGKEPDLGCSLTPDCQADEIDLGYTCLSISQQEIGKSVGCRPILISQYAPNQPLFCHPSCKYCFGTTDRQCLGCHEGMYFTPYLGMCLQNCSDDRDNIFIYHNTYIWKCELKCPSNLLTQGDMCVQSCLDGYVVLNGICRSINIVDEYFLSSEIVEIEGINQIIQSWNYCPFLCNTCQNTQICNSCINKYPFENNVCKISCFPRFSLKTNNLSTCKDQCDPNELTFWNDNLYGYQIAECFLKKCGEIEADQIYQSYLHQNDNKKCVYPCDDGYYGNSQSILCKPCIVSCATCIDKSHICTSCKPLLFLKGSTCLTSCGQNFKNYMNWQCEVECSSGYTIKDSTNNLYACVENCGQLYATYLYVWRGQCYENIPNISVFCIDYQCEDCHSSCLQCQGPNNNDCLACYQNSYLLNNECVQDCGILKHDQINWKCVEECPIESLQSIGISLVLNKQITICGLTCLLETFQFRNECYAQQPIETFCLQRPDYQFCELCSSLCTICSSSSSSDCQQCISTAFQYGTTCFMECPDEAPLKDTINKQCVASCPTGFSEDGYCVEFCQPISYIYQLKNACYTVGCPIGTFNETGSKICQDCYIGCATCFGNNQSECLSCIVGYFIDQETYCSDSCLIEPNIIEDQINRRCVQFCPIDSFLQKLINGRYGCKETCPEYYYSNICVSYCPSQTYQDGYACISCASPCSICFGSAITQCTQCDIGYYLYETTCYQICPDENPYANIQDSKCVQTCPNFLYLPKMLCFDSCPFFLYRYEIANQKQCVDNCLSKSYLLDQDCYPCDSICKECYGPANGNCLECELPYFLNGQTCEVTCPSFYDLTDHQCKAACPVNLVIQNIYCQTQCDQDYLQYGQNCLLQCPQFTYLNNNTCQNCNAYCLTCFGPNFSQCYSCIDGFYLNGQSCQQICDNYYDQDNKTCINNCGSKYLIRDQKQCVLQCPIGYISCEQECVTNIQDGFYLNNGQCYRCDQKCTKCTSSTSCSECSLNFYLELDSCVNYCINKYLYMDPISRSCVSRCPIGLYHQESFNRRFCMLDCTFKLDDQCVQSCPIGFYNDNSFCKLCPSECTECQSITFCLKCQIGLYLENNWCYPVCNYKKTDRKNQICVDECNLDLFEYENQCVESCPSNPIYFYYKSQCVQSCPTSTFQQEQYCIDCHIQCSSCFGSNNDECYSCFLGYYLDNNQICTQTCPFLYDLINQKCVFQCQKDQYLEQNQCVEKCNHFLYDKQCVDYCPSQTYQQNTQCFNCSNNCLECNSFGCIKCKIGSYLNDGICDSYCQIMYNDVKQECVEICTNQEYQYLDHCYSQCPIDTYEYKQICLLDCPIKTVQYNNYCYDCPERCDICLSEYECLKCNNLYFLFNGQCILHCPLQLPYEDTVNRICVSMCPPNTFIRNYTCLSTCNLITHQNMCLEQCPKGYYGNNICKHCKLECLACNDLDYCTECDINYYLEDNQCDNQCTNIKDLKTKKCVEICDSFLYKNICYQKCPIDTFQFESICVIKCPDGYYGSKDFICEQCPYQCFSCQNQFVCIQCNIGYYLFENQCLESCPDQSFSNPINNKCSNECPPTTFIYQNQCLFQCPNEYLHDIQNYKCVLQCDDQQYKEKNNCLACSIECNKCYTYGNNNCINCANLYNLNENGYCLGECPNGYYKNEQNCEKCLHKCQTCINQIQCVKCRGTNRNSIDCSCQKGYYDDELQENCQKCPCEECISQQNCQICKNNLQIPKCNCDRKLNDNWCITCEVAKVKIYYSDDLNEIVVYFGYLVSVNLINPFQPSDCYLWFDNSIIFGQNSKCYLAWNRYSVHIQLSPYSTINIGDELQFKQSFYRDVDQGNCSQQYIKQFIENKLLEPNRFIKPYIIFDVPQYVSSCKQIEIRQILIQGTAQKIQTVISWQLHSIDNEDYYLSMDSFLATQKNEIIIPINTLQPNLKYTITAKYINIFQRVNYTTFTFNTIPIQTPYIYLTYNPLTVNIYIFDCHITFSDILSDQNVQIDILDSLIQDQKYISIKQSIHSIRKIPLDEILLPKQVPLLFIAITQSYIIQEILILKSKPIEISFLQKNRFIGQDDQINARAYDKNIEDIILSTKDIHYQWLCTNLHDLQPCKTEENKILEFAQRRIINIQANSQNSTFVFYVKASKDNRWTIKEQLIVLLDLNIQEEFVINRDQPTDLINLNSEVTILLQQSQTYAFIIQNHKVLQYIKISNASLKFRMAEITQDSTHPIFIYLVPGTDSFGFKINEIPNIFTFSIEPKIGQSMDYFNYTIDNLNSDQIASIYYYVDYYTFKNDMDAKSLLNGIPLIFNTQITQGQFQIPSGIADDPIWIVCQIESNQGALIFKWTQIVVQRRNYEMHSLFEDLANLVNFSSLQSIHTMISLINQEDKQVCLKQCSGVGTCVDQKCRCPPGYYFNDCSGDKTQFENSNDLIFQSMQALIVKDALLYFQQYKDIISNTSIDKCLKVLINYITNLNNRMEQINQYSINMQYQQTNQFSYSQIDTRQILSIQDISNAILSTEFIWNKALSISNSSIYQIMHHLNDFFIHIIDLSFLAILPNEKFEISMSTVNLIIQRSNNLQNLSSHRFLQQDINTDYYDIVQAIYISNFYQYDGYYPYPNQKYPLYDYKIRQQNRYQNIKISKPITYKFEIHNDTTNLVCISRNANTYEWTKDNCYLSIINNSYFCNCTMIEPVTICNDYQFIYQGKPPFYLKLPNFIFIFYFFQFILLAFVIYKSQYGNKQQIAKSNKFGRVLKLNKFESVRMMFNNNLPSPISPISPISPISPNRIYPENDCIKETVQNIDQKVINKFNFNYFWRVQFIILKWNICFIVGEILSIFYFEYDIQIYILLASVIICRINQEFWKKQFKYYEYMKSILTLNLIKIIFFIFTTSTFIIGVYLYFWISDQTDFILSYITTNLIDLLFLDIIIFFANKYLGQTKKKLIKKKIQEFKLAMQGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNS4</accession>
    <name>A0A8S1JNS4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNS4"/>
    <dbReference type="OMA" id="HDKKETQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="349" mass="41264" checksum="179A5AAFA66EE23F" modified="2022-10-12" version="1">MQQSIQPIQYYEDQRPSRTILTTQPRSQTNKSSRSIKSNSTHRDQKSRISNQKYELISCCSCSCCGCSRKSSTRLKKTPTPTPKVRRNYQENPSTNKSNKSIKQSSIVSSESKKSRKQKILNSSQQIKQKCEYHHYELRQSHQTTHLSNRKSRPSSAAIINPETARRQKLKEYQEKQKQLQYLYSAKQLQPQPREQSLSKVAWRADISRIEEGKERKSRIEKIVQIQREILNKSLSKHRSQSKQESKKIIPPPDPQKLKLLNQRKKLMENKNQKIIHDKKETQRLKQVLNQLKKQKQDEKEQKTKKTEQLSKLQQYQKEHRLQCKKKKFEPNEQQIEQAFQIVSSDKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPD3</accession>
    <name>A0A8S1JPD3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0060416</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPD3"/>
    <dbReference type="OMA" id="QSQICIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="47"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="215"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="383"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="479" mass="58030" checksum="4495C788B71BC072" modified="2022-10-12" version="1">MYKPSRSLRQSQEKQYTNNKPSPQTRAVSFATTMEDPLRQISREFDRDIYKQKLKIQEVELLEAKEQQNQIYIELNEQKLKNKQQQQVIDNLEFKLHIEKKDSLIYQQQVQRLEEQYNYLQDNYNTQKQQIIVLQESIEQIQKKNNLEKEMRNEKELLQEKEIKFQQNEIKRYQEEIIKQREHMLLNNQQQNTNEFIQILENKFELVQKEFIFYKKEKEQEIEKIKQFIDSYQNQIECLNQENQNLQDKQLIISTKEEHQQQITSLKIELESQIQITKQLNQQIVILQQQLNQTNIICQNLKEEKQNAIKEKQAIETYYLDQLQKQDVQLSQMIKSSIQNEQKAQIQKLQIIIEQKDLMIRELSMQKDCNCNKQIKNEQKLIIRDLEKKNATLVMEVERLNKILKQKLTELENQTSRSCEENHKKYLEEIDIWKNKFLSINKQYHISQEQLMVVKTELDGLKKTKQKENIDPIQINKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JS01</accession>
    <name>A0A8S1JS01_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JS01"/>
    <dbReference type="OMA" id="VEYECEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052763">
        <property type="entry name" value="DnaJ_C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44825">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44825:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="116" mass="13732" checksum="05AD4D766664936E" modified="2022-10-12" version="1">MNYYELLGVDDKCSPENIRKAFLNKIRFCHPDKNGDAKEAQSLIEAYKVLMNCREEYNQSLRSQFKYCFDIMTVKCNYVEYECEQCGEENQIELNSQTNVIVECRSCNLKLQIFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JU83</accession>
    <name>A0A8S1JU83_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JU83"/>
    <dbReference type="OMA" id="FVEEPEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="593"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="593"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="615"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="630" mass="72559" checksum="732F5C10737F8192" modified="2022-10-12" version="1">MSTKKQVQGGKTSRSNSKQSVSNPISVRKRFSSLCSPSNNQGQSICVFCQKFKNKKVESYASLLNKALFAKYSSSQNYYYSKDINDIIDEESTPAVVFYRDLECIVEEEEYLKRSYAKKETTSKIKALLEYYKYHKDIPRLFMQKVYITINKFHEKKRRIEYANIKRRLNIPDEDIQKPKTKEKKKKQQHSDEDVTVGQLKHLLKDLKLDTYSYLQKKVDISSSVQLREFVQQIGQKYDYLGQISGLLSIDQSNSFLLRSQDLSMINKNLKQNYLINHKPKTNQNSKAQIIDSKTSYQGSLDKININRLQFNNQGEISSLKAENSIKAGEQTKRSNQEQSPLRNTSQLQTIQKVYSQIEGFANFVQKQQQSLQKQQKLNYGQKSNSQRKVSANFNSTDCQFRIPSRQNSTQKQSVDLQSYQVNFLRTRVASQLQKYDDDYKSKQPLATDRIQRQSVYNVGEKIQQKYSSKNIQKIPQKFTQYALATLRNLQVKNCEVKKKQIVNSNNMQGFTLNSQLLHNRTKSQDAIGNYKTSPPKQPQRNDNSKDPLRITNNICVQASSKKGGGVYNINQNNIVNIYIEDLKSSAKIKQGGSQTARINSPLKNNPKGGIFDVYNTQRSNLSPASKKMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXH1</accession>
    <name>A0A8S1JXH1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0040127</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXH1"/>
    <dbReference type="OMA" id="SPTWITK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000098">
        <property type="entry name" value="Copine-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000401">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF106">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="128"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="4">
        <location>
            <begin position="295"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="614"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8042908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="677" mass="77911" checksum="4D49085F5DF11858" modified="2022-10-12" version="1">MYNSQIEQSQFSEAIQKLELFISCRQLDDLDTVSVSDPYVIMYQKNNNYWTKIGQTELIWNNLNPNFATSIQLEYHFEVQQHLKMEVHHYISPTQSKIIGIAETTVAEIAGSKDQIFMGDLVNISGKKSGKIIVKADQVKQCNDELLLTLSGHSIPETRFWFWHGTCPFLRFYRLRKDDNNPVLVYETEFAKDTTSPLWKQISCKAQKLCNGDYQMPIKVELWDYRSSGKHIYLGETTFCVEELKEHSTQNKILKKEFKNKMKKNESSGVLQFNKFQLKTRYTFLDYCAGGQQLNLIVAIDFTASNGNPNDPKSLHYMTLNGAPSQYLQAIISVVEILINYDHDKKVPVYGFGCKPRMNLINTNQTLHLFPLNDNPEDPEVYGLDGIVQCYRNSLHHLSFDGPTYLHPILKNAMDMAQQCKNQGSENYLILMILTDGQTNDMQASIDDIIASSYLPLSVIIIGIGDANFKNMSILDNDDKSMVDSKGNKAIRDLVQFVPFNEFKNDPALLSKEVLAELPDQLVEYMELMSIPPKPPKNIQANQYQIPINNVSQQNIGIPQNPQFNVFPQNQNFQTDQESQQYISQQQIYQQYQQQQYPQQPQQFIPPQQYIPQQSQYPQQQIYPPQQYPPQQNYQYQQQQAPQTGVATKTFGQGLAQQGFLKGFVQQANQQFNYPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0Q7</accession>
    <name>A0A8S1K0Q7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0Q7"/>
    <dbReference type="OMA" id="QEDICAK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="433" mass="50110" checksum="16381E48A8B83BE5" modified="2022-10-12" version="1">MSITVEDLAANIDTVLASLGDEKMVNWEVVEQTETNLMKHFDQLFERLYTLKDSLQRVFLINIKNLRQYDAKDLAQKVKDKKLEPVVLKSLTQYAFKNDKEEILLNPSLIKGQVNSIVLPWVGNNLSVTDKIKETNEWLDQKLAELEVFKPKESQEQTKTQEIQPKSEGNDKSNYTFKSDKLNEIASQIQIMHIPSTNNMISIKTYTQIVEGSVDLAEPEFIRITLENRKARREVLYSDGAKYVQLLLDYVNDIENLLMKAQEEICKKIDISQQTLEQSEMLLMERGLGQHVFMLQASARQRIKDKLPKQKQVQMNATKEIIRYQIKLLNEKQDFLKNMIDKLPTTYESGQLVPMVLNLVMGDMIFEEHSYEEEDYISNLENPQLFQDPDMMELLKSIETGVVNLLGKTAFAQPPQGMMGNIPPQMLQRQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K193</accession>
    <name>A0A8S1K193_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130188</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K193"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8049019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="63" mass="7398" checksum="2AE6A75B44C15AA1" modified="2022-10-12" version="1">MKANLSKEFKVQIVFNNDILNELDAERPQEEMINRYCNLVESNKIKLKASMVMEKRVVVDLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3F1</accession>
    <name>A0A8S1K3F1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TIP41-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIP41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3F1"/>
    <dbReference type="OMA" id="KWASHIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007303">
        <property type="entry name" value="TIP41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF16">
        <property type="entry name" value="TIP41-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04176">
        <property type="entry name" value="TIP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="39573" checksum="3E2B88F94CE23D75" modified="2022-10-12" version="1">MNIISHKTYNDVNIEEYEQNGWNFIFTKAGMYNTSDLDMLQDALTLQGIPDITNGNNCGLIYNKQFNFGFLVDPKDALYLCNYQSRETNYLDIKKDVKSNKLNYISVIPEQAQLKYANIWNQKQNADIKVLNKISDTFYSSPYKGTIVDGDQINYQFQRFKQITGIQGKSIFEKQLNENVIKNEIDYLNQVFNIKSNTFHCEYTEDEIPLNNLTQANPIKWASHISLFEDELGDNGLSNCEYRFRVMGDCFFGLIRSYLRIDDVLIRIFDTRIYHQFEWDYVLRDFTHREDSWTNIQKNGFNFTPQWSTDQNQSFMIQNSVPIQLHVNDKIFFKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4Y4</accession>
    <name>A0A8S1K4Y4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0090404</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4Y4"/>
    <dbReference type="OMA" id="YWASQRL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF0">
        <property type="entry name" value="NOTCHLESS PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="274"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="276"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8045408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="887" mass="102040" checksum="CC115E2F82B30C16" modified="2022-10-12" version="1">MGNAVLCQEPKKKTTRGKQNDRTPKKIEISESAPQTQRIQQQQQVRTPKPQQVDNYNCQVHSDEPIKAVCMCEDCSHNNKILCVECLIDYSCSKKLRISDYLRHLRSCLSKDAQEAFMELFDEKNQVKSDLNRQITAVIEMVLTKLSSIGQQYVPILITNYYWASQRLPFKLKEQTIHQLELILENKANEAHPETLDFAIYLIQNRGVVETMCEQIERKKNKITEFSNDFVFKFNQVVSKYMTAIEFLFHLLTTPMEYLLDEDQKPQLMSKRIVQNKDQSNIQSRIKQNEDKSNITSPKESQQINNQQSQQQQKVNFKCLAHNQEDCQHKDRKMCADCLIDTKCVDRMKMSEFSARLSRILNKEIIEQLAIPWDDLSIHKGIEKLFSTAGENVKNLLTEYSLSVKEMFVKYHLIEESTPKELRGQFTKEYFDQFIHMMTVQKIASNNPAVILGIKFINNKQLIDNMLSAIVKTEQVLPKKADKLQHKLVSISQLFLEKIQNLMDLYKQHGEIEAIQKQYQLLNNEPFCLEESIYKIKQDGKSLSEWEPFHFFKPSDKNIIDVCFINENLAAIAIIDGTIIIYNVNLKKQTQILKHGIFVFSLRAFQFNGQSVLLSSGYSPQTGNSLCSWDLKTLKPTFIVSQAHNNSIRKIKYLHVNPQFSQNFFTNSTTTAQLSSQVSQTGSMNSIFLDIDEQILSCSDDGIIKLWKLDTNLKIVEEQILKQGQQVLDFTLLNGCILVSANNDKTVSLSLPFSNINPFQYINDDCLVYAVSPIDQLISKKKQSIQQKFITGNEKGIAKIFQWQNGRAVIMKQLNLHEDKITKISMLQPGFIASVSNDKSISLTLIQQAKVLTKIEKHEYGIVSFAYLKANGFMITADQGGNAILWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8E6</accession>
    <name>A0A8S1K8E6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8E6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="95" mass="11372" checksum="11E73169D6D22A07" modified="2022-10-12" version="1">MKTQVSQQNTPKAASKYSYKESYQQRTPLRQDNFLNLYEKYLNCQGRKRSHQMDEARSPLQQSTSLHYLNKKNEVGVHRFSLDNFNLDKRQFPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8T3</accession>
    <name>A0A8S1K8T3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0110152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8T3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="56" mass="6532" checksum="2E28E2E69817C05A" modified="2022-10-12" version="1">MLDYAFKTHQDTIYNSQEVQSIIDQIVEIQNSNLLINPTITVTPQKIFKVLFLNHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAU4</accession>
    <name>A0A8S1KAU4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0180287</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAU4"/>
    <dbReference type="OMA" id="QWSRCYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="3">
        <location>
            <begin position="474"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="1">
        <location>
            <begin position="474"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8051697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="553" mass="65245" checksum="2EDF2B7609F90D48" modified="2022-10-12" version="1">MDHQIQLKELMKRLHQQLKSAVTLQSKIQNLNYLLQQTSDFLDLFAQSNSMKIITNYLKDALKSIQINEELIDKILTLILKMNLSEEILRESEIAKPLLKIKDKKLFDNIRSNMIEQLQQQWSRCYIKFKSLLDEWKRNNNQKDKQEDRKKKGKRNQSSSSSESSNKKKKNKKKFVKFQRDDLLLNFKYFKRDDEPNQRGMTMEEVIQFQKQYAGEIKRLNQINGAYNIKHRESLMEGKEHKMQLDNIQQMLEQIPFIKPAKLLLQSQINYDTNYIETVIQQKRTESKMSAFYNRDNIPDQPGYNEISNTQSGLQPKIIHLDPPKREDLIYMVQVICNRGLKEIEEKNKNGNKKQVKGILKKPPEEQIKQKDVLNQAKQDLQPKMQQLVQMVEQKHQHSQESILTLLHDIIQKLKDIGEEKYIKNLKSIIEIKLNDKQTIKSEIDRVKAQQDQQRLRLEQLQTINPVQALRLRAYKTKHCHNFHSSIGCTRGDNCNFIHDSRYPGRPAPTLQQPNFLNKTLYPQSNMPIIVPPLLQQLNPAILLGRQKKYESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEC5</accession>
    <name>A0A8S1KEC5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PEP5/VPS11 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0190031</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEC5"/>
    <dbReference type="OMA" id="LMTMEFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048284">
        <property type="term" value="P:organelle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16688">
        <property type="entry name" value="RING-H2_Vps11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002610">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 11 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057308">
        <property type="entry name" value="CHCR_PEP5_VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057307">
        <property type="entry name" value="PEP5_VPS11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF24">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23341">
        <property type="entry name" value="PEP5_VPS11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23356">
        <property type="entry name" value="TPR_PEP5_VPS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PEP5/VPS11 N-terminal" evidence="10">
        <location>
            <begin position="10"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="8">
        <location>
            <begin position="400"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="830"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8052135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="953" mass="111204" checksum="B7A7CAC8B25E87F5" modified="2022-10-12" version="1">MRQTEVEQVKKFQFFNLENIQDQRANGDPTAPTLNDIGISDGCGIGTKLFLSSQSKNLIYVYEANSIQGFFQPHNDSFRVMKCTKESRDGKLVTFGIDSENNQKSSYVKFWDPNTTDYQNMKPIKVILVNKVGQPLQNFNCFCIAKDLSAMAFGLQDGQILIFKAKSQNLMTMEFKDQVIQTDQEPIKSVHLSRQDQNLNLFCTTDSNIICFQNMQNRKKFSIPAGALFDLTAKGTLIGCPKDDTSTIVEYSDTKKEATWNVDGDKIEIRFFKQNYLIMLISPRQDKEQVDQSVQLTIFDLLNNYIAYYKKFEKIQRFIPVGDYLYVITQNNRGEKNLIRLTEKENTHKIEIFFKNNYFDVMYRFASNQSSDKTLLAEISRLHGDHLYDQHDFQGAIKQYINTAGILEPSYVIGKFLDVSHVDFLIQYLAALHHEKQADKNHTALLLNCYVKQKQITKLEEFLKESSFDSDLFDIDTAIKECRQLGHIDLALRLAKSRQKNEAYLSILIETNKDQNNKEQNRQDCKSALMYIKDEIQLDEKAQYLKEFGQQLMKAEPELCLEIIQNLVLLISMVQNLKKRVDTQKGIESISILTPEELKVWRYFNLSDEEIKKVFSITFGKPDEFLHLFVVNDEYLESYLKFLIENCKTLPNEKAIFHRYFEYHLEKYQLYYKDESKIAIRDTQLQSKEQGIMKLLENQENEKKYDKNHLLVLFKMYNFVPGIIFLLKKLQMREELLNFYISLKENDQIINLCSDYGREETNLWIQALKYFAKPENGAENYIEKVLVLVSSLENLSPLLILNILSKNKNVNFKLVKNYFTNKISKDKKQIDDCQKVVKEKMKKAADLRAEYKKLKTQAKVFQSTKCNCCDAILSLPSYHFLCGHSYHEHCIHTERACLLCPQDTQMFFKRKQEFIEASKETKPFKEKLYAAADKFDVICEYLGQGIISNQKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEF5</accession>
    <name>A0A8S1KEF5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0200157</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NNPYDGC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035838287" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="2249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1848"/>
            <end position="1872"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1893"/>
            <end position="1914"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1948"/>
            <end position="1973"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2005"/>
            <end position="2029"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2049"/>
            <end position="2069"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2153"/>
            <end position="2172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2184"/>
            <end position="2207"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="739"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="811"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="929"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="984"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1031"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1106"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1223"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1331"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1392"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1514"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1636"/>
            <end position="1668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8053097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2249" mass="260052" checksum="07A8A9230090605B" modified="2022-10-12" version="1">MILFIEAFIILLTSLNLIQAEWRLIDSSFSEITIVDNNWKMKNSCSNGASGGYSVIRRTCGVNQLQYVRLQDEQQYLYKTFNYKSFQIQVIFDVFFDKANSNESQLKVYYRQDGSENTYDLYSREYDQDDVIQNSVYICKSSSSLFELQTVVSTIVTSNADKFTIEFCYNPNFEVEDDSYVKEIGVRNLLVFVNTCYPNCLTCQGPNETDCLTCFDSQDTQEGRCYCISEQQFSETFIGCRQECSRDFSIAGNDKICVNDNRIESKYSFFDDNDIPQSNDQRYTPLIFLDDKFHRKNADLVYESCNGKSLIGKMHFNEGMFYKMNLKNSVKFLRIRITFYLFNFQESSKIEILHNNQLQSRIIKISSGFIFDNLIKIFQNEKNCDASYTLLRIEMIFKLSNSDPTILLQGQLQKDTESWGFRNITIDTGFCQQNCKVCSDFSNCAECETTYYLYKNKCVLSCPPHSFNCIDYDQITPYSRYIAKGFYDLNMTIQEINKFYDSSPQPLINQATQQKFSFLNNKLVLGGLLVWNDGSYIKIWKIQQPHYAVSVYFNLTYGDGYIGQFYYKIGSTSSPYIGPFIKDGGGSNIIGRSDLESTSFFNIELTNFYSNDLYIEFKCEIEIPNITQEFCAISEYFIVAHYCAPFCTSCTNLNTCQNWETEHTNSNCENNQYLDFDQLTEIYDCKNCNQIGCSKCKSLDECTECVNNDFQLQNGICQCRPFTFFQDNNCIKCNKYCENCYGDSQENCITCVNDFHRSIQRNQCICQPGYYDDGINLPCLPICGDLIIVDQEDCDDGNFNPYDGCDNCKFACNFNCDICLNGKCYQCKTEYELINNDCLQICQNRNLVLVHQCNHSSDNCKNCQYKCSDNCIICHFGICILCDEIKGWYAQEDGKCNTKCGDARIVTLTEQCDDGNNNPYDGCNQCQFSCGMFCQNCINSQCLQCQEGYYLIQSNCYPQCKDEILVYPEQCENLNQKVLKGCFNCKFECQQQCQDCQFGICQKCKEGWYLQFDGSCQSICGDNILVPLIEECDNINNSIHVCSLCQYTCDVNCSSCYKGRCRACFQGYKLEQSIQKCVRICDVDSLVNYDEICNNENMIKNDSCGLCKYHCQNSCTQCTIFGCIECNSIGWKLNRQLHHCEPICGDGILVQNQEECDDFLETNCFQCKYFCQASCLICIQGNCFKCIQGWFLDLDKKCYPQIGDFLVVGDEQCDDNNFIMYDGCYLSQYQCQQSCINCIMGQCNLCEVGYINFDGKCLEIYGDQFIIDDEFFHFPQYFFCFLERKMLKFDNKLSKLDYFNNKCLSICGDGYIQYDEQCDDGNNNPYDGCYLCLFQCDNYCNSCFKGICTQCQFGYYLNKIKNQCLSFCGDGIIAHDEQCDDGYALVGQKCFNCKLYCQEQCTTCIDGQCFNCQQFGWQLNLISRICQPICGDGIVTGNEQCDDGNDFNNDECINCYQKCEQQCTNCFQGFCYECDIEGWTIYQNQCIPICGDQLIIGNEQCDDGNLIPFDGCHECKFQCQNQCIDCQFGICYACQTDGWILDHNNLCSIFCGDGIVIRFYEECDDGNEQPYDGCYQCQYQCEQQCTQCHNGICQECDQLGWIISNNLCIPYCGDGLLVGNEQCDDMNTIQNDGCYDCKFECNQYCVDCREGICNECQKGMVLIELLCLSLCGDGILINQFEQCDDGNTENGDGYWVCINIMHSQSMCEFERKPDFIINVLTSYSQEFQDIQIKFTQKVKLSSKVQNNLTQYIQTRIINSDRNKYNILDMKSAEINQNISKDIILSYRVTFFESIELPIFEIEFFNDSIISELNQTLIYNKKNIQLQTSIVLTQIQKQMAQVTSNFNEAIIISLAILSTICLLTGSSDVFWNLMDQLQYLSYIKYINIEFPSNLNIYFEVFNLISISPLISALGFDVLFNSIDGPTNYFVSTTNKFQKDDVNAYFLKNFSSIIFCIFTTYASYFSILSICYLLYKLSENQVIILGMYISKNILKLRKKLRIQLSKFYYNGLLRLILSIQYDLCFACTLQLAYTPKEENIILFLNYKLSQILFIFQILATFSIINIANALSKCNSLKNRQKYEALFEGISENHNFWKMQYNTIQMIKKQIFISLIVFLQENSSIQAISIAFMQTIFFIYTILIKPFDNIQEFIKIIITESLIIFNVLTFLFYHYKVEMSLTSETLIYMGWFNIFMFSSILIVSFIVDIIHQCQKLIRFIKEKFKQENKPSKPLFYVNEQNVSLNKRTIIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEK7</accession>
    <name>A0A8S1KEK7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEK7"/>
    <dbReference type="OMA" id="QIKIFMM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="58747" checksum="7D3F669048CC7C5B" modified="2022-10-12" version="1">MKYIQNLILTFENQNLEQQYQLEKQNQIQLPIFIFIIVLSFISNLVVLGFHFLNHKIETWYLNIAFSIITIILFVFIVIMKKYQYLQDAITISNIILGFLEFNVDPATSNYIEFYSYGNTFMQLQAVLYIVSNFGHAFLQVFFHFILRIAITIVLSKRTDYLLIFIGLTSGFIILVTIYYKERNSRKLFIQNLKENYWENNTSFIIQKPYFRINYQKEQLLFNLIKDNKIEQFPGYDNFYCEGCNVRKLLRYYCIDKNVTLEDYLLKQKHHHLSQKLIITFKKRKFLLKLCSLDIQQVQYLFILDEINIISKKEEDRKMKQNDFIQFLRSNKTYSYQHFFNWGAQSLLFLNTKIIKKINVQELMIQILQIFRQHIFHQISFQIIAQDENTSIFTFYYQIKIFMMQLFEILSIIQRNNILQSQIIIKRKGCDILIKITNIDLETFEQMFKSNFFLKNLQSLLLHEIEKQQGIQLHLKNYPIKSFVFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHR9</accession>
    <name>A0A8S1KHR9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHR9"/>
    <dbReference type="OMA" id="QGEDGHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="314" mass="37213" checksum="2767135B4BF08D01" modified="2022-10-12" version="1">MNKLQFVFKFCTESQKSIYKLENAQKYISDLVQKTDWISFAISKHIPKQVRQDYLNICQFHNELARIEETSKERSLGVGKLQYWSDQIDQIFLDNPQRDPLSICLHNSCKNNPLPKYLFQKMINHRKQQVERSEFANLVDMATAAEVNRGSQFLLFLRLLRMDIENQDLKSLTEYSAQAVGITDYLRRVPFTLQNYKLNLPEDIKIKHSINVRSLWDRIRGEPKEELYDAVLEVAALARTQMINANKFSNKIPAQSFRAFLHLIPTEQYLEELEYYNFKIFEKAINQNSYVQLPITLFKNCRKMRFLNKDYESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKI5</accession>
    <name>A0A8S1KKI5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-disulfide reductase</fullName>
            <ecNumber evidence="1">1.8.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0220264</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[protein]-dithiol + NAD(+) = [protein]-disulfide + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.8.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[protein]-dithiol + NADP(+) = [protein]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18753"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the nucleoredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKI5"/>
    <dbReference type="OMA" id="WPAIPYN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047134">
        <property type="term" value="F:protein-disulfide reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052259">
        <property type="entry name" value="Nucleoredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13871">
        <property type="entry name" value="THIOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13871:SF96">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13905">
        <property type="entry name" value="Thioredoxin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8054901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="144" mass="16756" checksum="5AC0EECFD609E0B4" modified="2022-10-12" version="1">MDNNFFGDTFVNKNGPCKIKLSDMKVVVLYFCASWCPPCVNFTPTLVEFYNDVNLETKQLEIIWVSYEESESQFKKYLEEMPWPAIPYNDKRIQQLVDKYEIKGIPTVTVLRKNGDIAKKNGKQDILKEGEGAYNLWEQLVNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSN2</accession>
    <name>A0A8S1KSN2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0210239</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSN2"/>
    <dbReference type="OMA" id="RATMIDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8053984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="50886" checksum="98B390599ED31E50" modified="2022-10-12" version="1">MVFIKRLVPLSIFYCFEGIMLGLILYSVPSNLIDKGVEINRYYFSFVVLLPYLIRYIIAPLIDSVSWDKIGRRRSQLTVLYFGLGVLLYFSNFQSLDPSTWFWNTFWIVCFLACIDLIIAAWLVESLDKQDRGYGALAQFLGILIGGFIGAYIFKMFNSLEFCNTYIYAIPQEIPYITLQIAFKDLSYISVGLAVACTIIIHNEKTTNQDIIEAYKSALGAFSNRHLIVLFLFFCFFRIGQMPIDLAHIHLKRGQFTEGQIQFLEILTFPIACILPYYMAKMIKTRVLEIRLIIVFSIYEIAISGVFIITLFASEENFPFPYRDTLLKISLISVWLLNLINITLAQSFIYKSTQSSIAATFIALLTTGINLQFIFEGFVEYLLANYNYYLIWICGVGIYALFFFHLASRATMIDQLNCQEFCLEIDIKKEKLLINTELATLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KST8</accession>
    <name>A0A8S1KST8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0230335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KST8"/>
    <dbReference type="OMA" id="YHIEFEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035924806" evidence="1">
        <location>
            <begin position="16"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="587" mass="69406" checksum="B6C92A6D75CED8ED" modified="2022-10-12" version="1">MQLIFLFFLIQWIKCWNVITSKKIIESIKNDYLIFEDDYQIVANGSYSIKYEGVNGYRRLHIIFDAKSNFSQVEILIKVNGIKRNFLIIEKDFKEYHIEFEHQTSDIKIEFIFDDQIQLSMRNFNIFIEECPQNCKFCLSGFCDLEIIDQCEKLRFKEQCVDECPKGYLSEFRECIKTSLNQISLNQKSVKNDFGSIKRFGENQELIIDFEQNFQGWLEIVFNCYGKQEDHYNSILKISKQNTQSETIHPFLPIYYNTQSYKYCKSRFELDCIQVKGYFKMEFQEQNNIMIQSYTKNVENSGYWNIDEIKLYDKEIKYYDCSINECEQCSFNKVCSKCKQNSYLNQNLCIKQCPYFTIQKDSKCINIDEQQQDVSFLIKLVSPFQDFLQIFGQKTFDMKEQISFRYENGTTFIGGGLKDNWRRTTFTKQLNLGKHYQIRLMFNLSIENSTSDSDCFTYTIDGKTFLVHQNVSYIDRTLYHNKNILNMNLGCKISSNGRCVISNYYFMTMKCSPLCLQCVGSLASDCRAFQKDIDNFDYLKLKCLEGYYLDNYGCQKCSVGCQICESLNKCLKCKDDKDGQFYCSPYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVT6</accession>
    <name>A0A8S1KVT6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVT6"/>
    <dbReference type="OMA" id="NYYLPKC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="166" mass="19686" checksum="9B57BECF512E7779" modified="2022-10-12" version="1">MFEYYAIGKESNKIHDIHKYLLALCNKKMVEKLAKTQNKQQRVSTSNEQKIRPSISYRPKEKYPTLIGAQYKYFKAQPKRKQIYLSTLQKDQSESTPRLKANSIIQEQKIENQMNSARAIKTAYQQNERDVQSQKAEKSPKNYYLPKCDSNWIIHLKNIRSRQEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KX15</accession>
    <name>A0A8S1KX15_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0270212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KX15"/>
    <dbReference type="OMA" id="DIFWEHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF83">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="505"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="542"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="588"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="685"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="739"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="784"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="865" mass="102707" checksum="7B6B556C3B72A4D6" modified="2022-10-12" version="1">MAEQIDILDFNEAHRNRNSQNQETDRFSARKIKQLLTLEDVDSWVEQINMPHTRNFEFPSSIEKATIHSQSFYIHPNKKFLKENPKIINCDCCGQMIRRKRYNIFTCDMYSIFAGEYGISIPLYFSIIKLHFFILLAIGCIFGIYYCYLSDKYCSQLNQNNHTQKELYEQYCASFLAAQHFLLYNFNQQEIINKNYGEQYTFYILGYVAYLIMMMTPLMHEVLLRLQQIKYWNYDSLNHIRIPKLTLYIKHIPKKFNEQELQLYIQNAIASKYKDKIGGKFIEEIVYIYDIHPIKVLNEKRKETYLKSLQIVNELKINPKLELKGQLIDLVFQLIKQTRDIQKTLQYGLPFTQKVILKFNSESVTNLVYKFYDKSFFNNLLQKIETIKESAQNYQEFQKDKFELQAQLLEQSETNSSKSSIISQQSDLQEMVRSQSEIFNQPSELKARMKQSIIDLLRLPENDEQQEQTMQQSTIDEKKGLHLQRGFRQDDIFWEHIGMHTLKRFFLRLITTCITLLVGIIIMALFEILFILEQHLKRQVGWVSSLIVIVMTLFTVFLSVFSTILVIFMTKRSQRSTYSLQEKNIITFMSPIQQVCILAFPFIFVLSILQKHSALNSIAVVLAVSKLAQVRLISKGLIHFFHLRSVKFNSIKKKTMKNFMPTSYFQKQLNQLLTPPLFPIRSRLFYILFIYSTGMMMIFFNPIMIVWCLVLQIIFFYYDKYSVLNEYHYDKLFTFQLQKYLILTYQVIMIPIYCYIYVVTFTNTTSHIGADGSLINEYSTVSSLWTFLGYGTCVLSFTSFLFFRQQIANFFVFVVLRYQKPLRDSTIFNEKSYFQSYNEYFHDLGVHELNTILEDTFKNMQYKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYQ6</accession>
    <name>A0A8S1KYQ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0240098</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMC family. SMC2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYQ6"/>
    <dbReference type="OMA" id="ECAGTTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03273">
        <property type="entry name" value="ABC_SMC2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027120">
        <property type="entry name" value="Smc2_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="7">
        <location>
            <begin position="512"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="171"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="243"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="432"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="677"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8056014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1179" mass="138266" checksum="7DBED587C9536D10" modified="2022-10-12" version="1">MWIKEIIIEGFKSYAQRTVITNLDPEFNAITGLNGSGKSNILDAILFCLGLSKEYDTLRIKKLQELIYKNGAAGITKAEVTIVFDNRNKEQSPLGYQDCDKLTVTRQITADKSKYFINGKSETQKNFKNMFRSVQLNIDNPHFLVAQGRITKIINLKPQELISMLEETAGTSLYNEKKREAQKLIQKKEEKLKQVNEIIEAEIQPQMQKLTDEKNIFQLWRAQEAQILVLKKDLFSYDYYQKAKTLRLKKNDLQIVNEQITNQEEKMRYENAEISTIQEKIQSLQEQNRNNKYEQITEKYKEKQKLVNTLEKQVQNTRRQKETIESEKIKLEHALRTYQTDKERTDQKIEIADRQLKQVSDELKEKKDLLDEQIGQQNQSEDGNIAQNGKQMIQRQINDTISHIDSNRKDLEQVTERLQRIDNHMLSSKTIYEQMQKEATNLDTKIDLLKKRIEQSEIEIQKSSTLEQQLLDYKSNRGDLDQQLLEIKKQISQSQPFIFQLNLSRMKDWDQNRVYGKLFSLFEVKDEQYMKALEFGAGAKLQNIVVDDSTTSTYLLKNNVLQTHSYIIPNKEIQQSEAKKEFVQAAAQIAKENDGFAKPAIDLISFSDKVINSMKFVFGNFIIASSMDIARKIAYHPSNVQKCKVVTRDGDIVDPSGTLTGGYTNEKAQLLPKFKLFNRWNQEYKEIQGQIDKIELQIEKIKQDIEFKEQLNRAITQDKYQLEQLMIKQRKSNQFNFQTEQNKYLNEIQDLQIEQDRFKKQIKEGEDKLVELKKELQLIQQGKNTKELIQAQIDRTKKEINKLKQQIDQQKKELIENQVESQNYEQEINKCIKKIKEETNNLDKTTIALDQLINELNINKDQYLKITEEKNLYESKNAIHNNQMTRLLEQLQERQKYLNSTVEQLNGHQNELKKLEREQHDLKQQLKQLEDQYDFIRQDKNELSQDRLSDKFRVLETLEYEKTKQQFQRLEHDQGKLGKQVNFKVEAMTEQVEKEFQSLKDKKLILENDKSMLIQNMGELDDKKIKTIEKCFLEVNKDFSSIFSSLLHNAQAKLGRLDGQSIEDGIEMNVSFSHQQKNLSELSGGQRSLLALSFILALLKYKPAPFYILDEVDSALDLSHTENIGQMLSQNFKQSQFLLISLKEGMYQNANVLYKVQFVDGVSKIDRHELKKKKQQQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L159</accession>
    <name>A0A8S1L159_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0310238</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L159"/>
    <dbReference type="OMA" id="IIMLIPE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00105">
        <property type="entry name" value="KH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="109"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="279"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="394"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="408"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8061297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="508" mass="58464" checksum="227521CF8CFE7422" modified="2022-10-12" version="1">MSQDQAYDQGLSGQSSQSPSHQQNQKRMNQFSIDFFIEKRFINQDDLIPILEEVRSQYNVESIKLNIQNQIPALEGTAFSIIDHDENKLNARCDAVNKILQIIEQRSKQILEIIMLIPEGTVSYVIGTQGKQIENIKLETKAHLVINQPIYEFQLRTVTIIGDSSRIFNAIKLIIKQLQERGISNEDYAKKAEPLDPRRVMTKAKFAFLTMIIEYILKNRELEKKYSVRLKGKAINDIKIKKNKLKKDEGILQIDGTLFNVQEAIQNIIKKVTQQFKKNEFDIRIVMPSNFASKLIGAKGCQIKELANKARGAQIKVLSDRDDTDVGQDCLVQVTGSMENKQEATILILEQIECFKNGGPILESGKYINENFAQQYKNSVQVQDMKQKRFVWNNQIGRKQSSSSRSDRRQRSRSSNKRQKYIRRSRSSSSHKRKTKQQLLKTKIVVKQSLIEMMHKQLIRFCSDFNVNIQTFPSDEGNETIVKINGDIKGSIAVIQYILSEQCKLSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2Y6</accession>
    <name>A0A8S1L2Y6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0300204</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2Y6"/>
    <dbReference type="OMA" id="YNRQSIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="215" mass="25545" checksum="33F5497252AD2D2E" modified="2022-10-12" version="1">MNNNHYYRPTLTGNKNRRRVQETNFAAILKMHSNKNANLKSSLLFHNPKDIDTQFNLINRSIDVPRLNDRSASKQRVKTEIVKPNIDGQFQNQKNDYTTAYFPRLKERIAQSQKKIFQEKAQNDEIEDSPIQDLQKSLDYSNQAYKIRNLTPIQQRTLDIPKFQSKSDHLNQEEIMQFSFGLQYNRQSIEKQKPRFPKDIFLGDVRKVKRIFQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6T3</accession>
    <name>A0A8S1L6T3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0310216</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6T3"/>
    <dbReference type="OMA" id="ICTDHMV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009082">
        <property type="term" value="P:branched-chain amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000017">
        <property type="entry name" value="Branched-chain amino acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000027">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825:SF44">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825">
        <property type="entry name" value="SUBGROUP IIII AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5 8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8061253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="398" mass="45164" checksum="DA0CBB65689F281F" modified="2022-10-12" version="1">MLRNISKVSTFQTHFKQIYSFSYYANLDLSCPHPSFQSRDLTITHTMHYITKPPCDPKIMTFGAFHTDHLLEIDWSEKMGWSRPQIVPFKSFSIHPFAACLHYAIECFEGAKAYRGANNTIRTFRLDCNMYRMKQSAKRLSLPDFDGAELQRCIEQLLKVDRDWIPDRPGFSCYIRPTLIATEEALGVRASSRARLFVVLCPVGPYFPSGLKPVRVFCNTSTIRSAPGGVGGYKVAGNYAPTVLPLKEVQKIGFHQNLWMLPDGLVQEMGVCNLFFFQKNEKGEKELVTPMLDGTILPGIMRDSILEITRGMGKFKVIEKKLYIQEVIESIESGRMIEMFGSGTAVSIQPIEAIGYNDKIYEVKYDAKLNAGELSHELFDLLTKIQTGGEDHKWIKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L721</accession>
    <name>A0A8S1L721_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0350085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L721"/>
    <dbReference type="OMA" id="EPNNTEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="133" mass="15895" checksum="989F9A3AEB6A6CB6" modified="2022-10-12" version="1">MFGEPQILSPVKRQFIMQRIINNMNEISKPESTSPVKRRIRIQSNRIDYRKNMSMVENHSNKQITEPNNTENKKLFHIKANRKMSQDQMHPKSPPMLPSLFEITFQNPFLGQFSQRKYQRPTEFQAIVYKRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L845</accession>
    <name>A0A8S1L845_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0320185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L845"/>
    <dbReference type="OMA" id="DAMFFEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8061822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="269" mass="31132" checksum="FE86EF952AB35A00" modified="2022-10-12" version="1">MQYHYPQANYFPQQNYVSPMQMDAGFNSGLDALSRCPSVFIKQRPDYLETLGLCEKKNAYFVYQSDSMGNKPDFKQQAPIFKCKEESSCWQRNCLPGACRGFDLKVKQYNERQDTNTVFRMSREYKCTFLCFGRPVMEVQLENGGKIGSIYYPFMCCDKGVDILDENDQIVYQIRGSCCQWPFLVNLPCETCQRARFDIIDSQGQKISELWKESAGFCNALCDVDATNFRLLFPPQSSSRQKALLLSAALFIDFNYFEESPQDKQNNQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L8F4</accession>
    <name>A0A8S1L8F4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0280144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L8F4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="247" mass="29719" checksum="F3689ECE99B0CDEE" modified="2022-10-12" version="1">MSGCGSREKELREKVGFSQSSQDYAIKKFSNMTKQKPLYFQFLQMEYRRIIQFWDFKQYFTENPVLAQNSIFLQEIMVDKIMSKLLHCIPEFILIFQELKENLYSLSIQNSKYTHLIVNNKVSLHYQFQLLQKDKNKDQNNLVQVQEVVDMNIIEFGQMVNNYKQVDLWKKTKKFKVVQYYYQKYISFKLIPFLFEDHISPLISSRLKDSTYLQIKLTPNLLISLLRIHNLSAFIIENRYSYQHLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L8J5</accession>
    <name>A0A8S1L8J5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0340033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8062871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L8J5"/>
    <dbReference type="OMA" id="YADYNIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="343"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="253"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8062871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="51615" checksum="32DBF215439E728D" modified="2022-10-12" version="1">MNQQKHFKFILQYLEFSSKSDQQSYELTILLKRSTAAAQQVVTKLPIKNPERTMTICRLPIDQVVEFQQRMIKKDGIYADYNIKFLFYLAGNQLLGDCVINLAYFLDGGAPQDQKQKKPLTSTTDKRAALLFKVIKDSMFESQELPIADIPAPVNGPAPQTARTSSTRKIDSRTTRARSQSPPKEFKSTIMISKANEQPVQQTVSQSQFQQQQHQQIQQDNLTQKFVQLGEKFQQQKLQQTIQPTTSSPPPEYDNFKEINDLLSQQEAELEKQIEQLDIHIRSDGKFKKIFDDSTFNNFDVSSQDTEIQQWKQKCAELEEKFNKIQDENQHLTVIIRQQSEQLKKNNQRSQSGYKQINDNTLNQSTTSNQEQYEKTIEKKNQIIKELSNQLLELEKNANVNVIDQLKSQILRLEQQMQEKEDQWLSQERIYKDKIIQLLQNTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9G7</accession>
    <name>A0A8S1L9G7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PARG catalytic Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0340025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8062855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9G7"/>
    <dbReference type="OMA" id="PMNDNEA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004649">
        <property type="term" value="F:poly(ADP-ribose) glycohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990966">
        <property type="term" value="P:ATP generation from poly-ADP-D-ribose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046372">
        <property type="entry name" value="PARG_cat_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007724">
        <property type="entry name" value="Poly_GlycHdrlase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837">
        <property type="entry name" value="POLY ADP-RIBOSE GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837:SF0">
        <property type="entry name" value="POLY(ADP-RIBOSE) GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05028">
        <property type="entry name" value="PARG_cat_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PARG catalytic Macro" evidence="2">
        <location>
            <begin position="244"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8062855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="514" mass="61743" checksum="6A7CDC698E9E4E76" modified="2022-10-12" version="1">MQLRVRANKQEYFDFSNISNDKHFMVQFNKIANQLIKDNQVNLINYINEFIIQQSQTKIRNNEIKQNIQKEKSNYEQGKNYNQSFIEQQIQFTRKQVFILLLMMYSNAIDKDNQNMQQSGFYQINMELIKQSQCKQSQEKLKCIHSYFINFYNRFLKQDQVLNNDQQLNQNLKQIQDLEKEIQEEEYKHSQKIQYLNQKIEMIRNQLLNNNNEQDEFVTYIRRTEFRQYNQIFNDQQYLLSYFTKNKGRCEDYQNSVLVNFADKNVGGLSLDTLNIAQEEVLMLTHPEALISMLFMKPMDDNEAILIKNVIRFNDYDGYGQTFRQKDEDYFKLIQNSSFKQNNEFSQQKEDVNKLTQNKKIIKNHILCMDALYYKNWQLQFDQVCINRELTKSYIAFSLALDYTNNENISTGKWGCGIFNGDVQLKFLIQLLAFSKALQDNKQKNEQNKIQDYKNKTERMIIFSAFNDNQFDDLIQFYENALMKKDQKYCKTIIQEIENVKLQIKQQNNYGPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBV7</accession>
    <name>A0A8S1LBV7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0350237</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LBV7"/>
    <dbReference type="OMA" id="WMTYKTA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000100">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="6">
        <location>
            <begin position="207"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8063981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="492" mass="54410" checksum="D6905A7DD4C3E926" modified="2022-10-12" version="1">MHIQKISRSIINRSCFSTSAVNEGTFLDQVMAYFNKASGHTNITQDKLDFYKSTDIVIKFNLPLVRDDGTYICVPAYRAQHKTYRLPTKGGTRLSPHINIEEVEALSFLMTLKNSILDLPYGGAKGGIGINPRKFSKREIETLMRRYTLELAKKNFIGAAIDVPGPDLGTGEQEMSWMKDAYTKFKGHQDINALGCVTGKAINQGGISGRQESTGMGIFFATREILNDVKFCQSVGIEPSLKGKSVIIQGYGNVGSFCAKYMYDYGAKIIGVAEHDGSIFNPNGINPYELAAHKTKTGGVKGFVNATKYWEDEQAIYQQCDIFIPAAFEKTVNAGNADKFNCKIIAEGANGPTTMAAEEKLLSKGVIFLPDILLNAGGVTVSYLEWLKNLKHINPGRMTRRWEEQAKHRILEVIKMSTGLNINIKDSKLAKKMLEGPSETDLVHTALEQSMIEAVNNIMATSKEYKVNLRLAAYISAINKLNEHFEMAGIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LE71</accession>
    <name>A0A8S1LE71_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0380249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8066060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="THECQNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035875209" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1263"/>
            <end position="1283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8066060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1305" mass="155854" checksum="6B40CCAAD3C5A571" modified="2022-10-12" version="1">MITLNFIQLQTVLFILCLIHKVQSNNDQKQCQTVLLKQNEKYCELREINTFFVGQRSDNLTNINTLQSYEDLIQISNGPILYSKDDIIISIKFRELIDENEICSFIYNPQLNTYSINCISTQPTYIIDNQIIIQINNQFNILIQTEQNFNCSQFDYNKIGFAIFCFSSDQIKLYQLQKEQEQFKQTIIYQSEILNLSCLLKFHYYSNIFFIIYFSCQGWIIHVYENNSFKLQIDQNLLSEKYNTEGILLDVLTCQHNIFMLLSSGGYYYHKEQFILLFKAENLNLKDIVFEFDFHCNLRTAVKSQSENYVIYSLPKKFQEIIFNTKELPQKVFQISNPPCILLKYENYVSFYVNELVYQTITIKISHLIQLSNQRIYIGIGEENQIKLFQIKYSKPCLPYYNDNQKYSNYYLQFIDKYNLNFQKISFIECYKPIIMDNDELKKEYAIFINIQNQIYYIQRINYEESFQVSFLASSIQSIQPYKINFQSPLNQIKFQVKNNEIKCIFNHQLKNYKEKFIIKTKNDLIYSIIVQGPKNNIIEYHCQSSQDYIYQNILPLNEQLLHKFINSNNLILLDQQKMCLYKIQLDEQNQNELISKILCLDSQVEKIFSLNNQIIFKLKDQPVFYNMFSNLGQIKIEQNFMLGQIKNQNFQDIFQIQIDFYGVLYKDQLHLIHKQVVVQIINIKAEILGFLSNSQSNLISSLVLIDIIKNELQFYVVSTTEFIKIRYYNLGNYVPVQPLQFKILESKIIISCQHKINKKIYILIMNCAFQSSLHLLQKVIETSNQFFWVQRFYIYYFDNKNELSFQDLLHINIEIQYLEFDNKISQEIIIQIEIELENIEIPPFKKDIMITLINPKILKLIQTDYNLLINNNNQEVNFRNFILGEIDNIILKSNDEFQLRYPLEKEMVQICQYYCNKFCFQVNNFEYMTFFKFGLFENYLVYNFQKQINNLLFVEIIKQDLFLFINQINNQTILISLMQIKDNFFIIQKQLNTQIFINIKNIFLMQNILIIQTIQFECQIYHITSDLYLEVMINSFQKVIVINLFELFNSKLIIYRKFEILIMNTSMKNLFIDFGTPILQDSIQIIKQDYNQNKQQLDLILFIINKQVYDIQFLIRLYLNEQKYEFMNYKILRYPKSIQSCKYILLSETEVILECGLTRYFYDIQNSEFLCDSIYSSKILDQMEILNSTHYLFKNHFLNNQSVVLSIGKRGGYSLQKQSQYNTESISLEFQVISIDQMGTNQVNLNLQVIQKSFEQNEISQLSIIILLIIFIIFLATIKILYRQCKQQKLSNVIKKEISQTELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFM7</accession>
    <name>A0A8S1LFM7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0350008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFM7"/>
    <dbReference type="OMA" id="HARIRWA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="228" mass="27123" checksum="1DE8555948D28112" modified="2022-10-12" version="1">MKNRFKVILQPQNQDLYLEADQSISVEELFEYIKTSLKIDNDLKNWNCYSKSKCMYLDLKSELENVQNDNLIINTQNFSNTHTGMHQDQQSINNQHFQTPQQQINNQYQQKNKKQDQINSYVFNEIPNSNKLSINFVIRDGSIKRQFPRQFLRQFSRQFNIHDKLIQLKDAVMEYLGFESDDEVSCDLYIFGQHYRGYCLEALTLHQLQLKYNVEVQIVLRQIGYSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LG53</accession>
    <name>A0A8S1LG53_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0370036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LG53"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035718401" evidence="2">
        <location>
            <begin position="20"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8065002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="198" mass="22842" checksum="5034A2C0DAD3F074" modified="2022-10-12" version="1">MFPYILALCLSLCIQIVNGVSDNIEVITSCWLITVQDNQKFKTFDHVVDSVAKRYQQNDKDKIVHDIRIYMIKKCFATLPLADSKQIIKDFEAGLSFHVTYREIVKGFDYTHYIKDLKNKDLIPNPQLDDTISNIVKTLETEFLKDTQSEIQSTVPPIGVFGMNFNYLIVFPILGGVFLTYFLIKAFKTLQTTQKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHE1</accession>
    <name>A0A8S1LHE1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 10">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="1 10">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0400175</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="1 10"/>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHE1"/>
    <dbReference type="OMA" id="MDIPRPE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07852">
        <property type="entry name" value="STKc_MAPK15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000238">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000166">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="13"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="353"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="416"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8067227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="543" mass="62172" checksum="1C21475ABB231F11" modified="2022-10-12" version="1">MSDEIEPHILKRYEIVRKIGKGAYGVVWKAIDKRFKNTCVALKKIFDAFQNATDAQRTFREIMFLYELDHQNIIKLYNVHRAENNKDIYLVFEHMETDLHAVIRAGILEEVHKQYIIYQILKSIKYMHSAELLHRDLKPSNILLNSDCSVKVADFGLVRSVACRQDAPSPILTEYVATRWYRAPEILLGSHTYTKGVDMWSVGCILGELLTGKPIFPGNSTLNQLDRILQLTGKPSTEDIEAIQSPLASTMLEAINPPQVKLIHQLFPTASDDALDLMYRLLKFNPNKRLTAEKALAHPYFSKFHNEIDEPSSDKVITLAIDDNQKFSANEYRDKLYEEIATRKRYSIRNYEDLNSQLHSPPPISESNQIRDRPQTQEVKSQQQQQQQDSAKLLDSAKQQDSAKCYDSGKQDPLKQRNSGRSNSKQSKFVQSALNGLINPRQSSKPTKQFLQTQQQPNKTYAPSFPNTSSITIQKNVSYTQTEPKVTSQQLYQQYYKLLNAAQDKLQGNQESAQSQQKVKKGLSALFGSNQNLIRQSQNKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIZ7</accession>
    <name>A0A8S1LIZ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0410110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIZ7"/>
    <dbReference type="OMA" id="HVRHISQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="137"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8067680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="245" mass="28989" checksum="664C63E39F9F0249" modified="2022-10-12" version="1">MQKTNSYHLKTNKKHKSSPLTSQELLQKLLDFKQTQTTMNCSYENKTLVMGGNLAKKISKSISVGSRSWKDIVPHLHESDNCLKQKEEIVNTLKRSKHVRHISQIWSMRNRTDSNFHTPRKEGVNDFIQNARIFLKEKLLENKLDKLCYQVQQLKNKSNILELQNKLLFENIKQHHNEHNNMKERKVLMGKLDQMITMQKKQEQSINYFKQLFKDNDDEGRRVKTQTSYPKLRPVLYSGYIGLKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LK79</accession>
    <name>A0A8S1LK79_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0380037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LK79"/>
    <dbReference type="OMA" id="KIYMLCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="152" mass="18076" checksum="F186A6455B8F3158" modified="2022-10-12" version="1">MERIDSFEGKLFYKIYMLCKKNQINEEQKGKLKDLVILRDERIEIAMNQYLEDKDEFRLLENFHNYSGEEYSPKSFNNFSDDVSVGSHSKFKGKRPAKMELPKKTFMINLRKDTDVTTYSKGFHLSTARSSDNRNVLAFNERYTQQNWSECE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKU3</accession>
    <name>A0A8S1LKU3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0400109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LKU3"/>
    <dbReference type="OMA" id="YLYQISM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="23909" checksum="0240CA29394FD7B7" modified="2022-10-12" version="1">MQRKRRCAFDSDSLEEYIQLATIGQNQTNLFYDDKKLNWLNGRKQVDDDEEIIKDIEGFEHNYSSPKVDYIDTSELPPDIVLKSSDDIFSHHIMILGDINEYVKQSLTIFCMQSRKNSNEDFNEELISPIKSKLCLDLAIKRIQEKNGVHIVKFLMHDMTIQNYGPIINGISISRSQFTFSKLPITYLYQISMRIIHKLRKNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LLT0</accession>
    <name>A0A8S1LLT0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0420300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LLT0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8068717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="32938" checksum="3130DE56152618E9" modified="2022-10-12" version="1">MYRKLKITEFLQFKSPQKQYIKVQEVTQKTDSLLVLGFFQIRFFYKKYQLKITRQMNSFIAYQITLSVYGMSKQDNKKLNQIVIMIVSTQSVSLLMEIHQLFLIMITPSVYGMLKQYNKNLNQNAILMILDQSASLLMEIHQLLKVEMALSVYGCLNRIRNQRLKQSIQRNIGKLHDLVVIKQPFYRYLVFIFFGQHYYSYSLYILNTNLLSQRSSCPKWRVYQLVRLRFKIVIKIKRMLYFGKKNATMTFYYFFENQYILKVYFLYFFFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPT0</accession>
    <name>A0A8S1LPT0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0400258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPT0"/>
    <dbReference type="OMA" id="QRQAYPM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="237" mass="28259" checksum="01360CCBF35CCD8F" modified="2022-10-12" version="1">MINDNRNPGFFQQPNQNQYNIILPNNNQPIIGQRQAYPIQPPTQSIVQQPLQFQQQFPLPPQVNYVPQTIQSKPNVLPTVYQEKPTQVINMDEIEAPWRLKCATLERQILELQIQLRKGPGPTEPDAIEVIDDTRLRELESKKAQLQQMIQDRDREISELENQIHQQQESIEIQIQERTVHYSNEIETWQKRFQQLERDYQQSQRQLQDLQEEQKRLEKQNQDVRTSQQRVGQSRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQI2</accession>
    <name>A0A8S1LQI2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0450017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQI2"/>
    <dbReference type="OMA" id="VFHERLY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8070017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="264" mass="31415" checksum="4C895D35A278863D" modified="2022-10-12" version="1">MLFVSAVQNALSSPKYKSAEKQKKQKPSIDSEQLTTLLQLEAVFHERLYNIDHVTKLLEIYSYLVELLEPNLQSLKDYFLEKMEFILSRPETIEIMMENDERSTALQNRYTTLINSQALDQLKLDEEQRFNRPFLQQNSDSNSKMRFSVFKKHQAKEREICMEVQERKQDQKGDLNSLLQEYDQISKEYDNVIKKQLQDQNNTINQRMAIRKNKKKRTKQSNSQENKRTSVVNPVRTTLSKWKLITEIQESAQFWRQSINKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQZ6</accession>
    <name>A0A8S1LQZ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="203" mass="23978" checksum="A59DFF90A2DCBCC5" modified="2022-10-12" version="1">MEISQMKQETIQPLFKQKYENILFIMLNFFQSISCFSNREKLDKTNIFIQQRAQTMSIQPISYKNTLDQVLKNDIIETDQYLQLDNQNYIESFDFESSSGIPSTQQPSDFMVLKDGKLQQQIQDKIEQNAQKEQNFTRRQQNFGIPLKMLNYVSNIKSIMENGIKLLNICQEEMFHNVVKDGEDCNLLRFLKENNGLKKKXRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LRT7</accession>
    <name>A0A8S1LRT7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0420266</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LRT7"/>
    <dbReference type="OMA" id="FIDELIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF94">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="77"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="253"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8068651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="391" mass="47160" checksum="3D47082C79FC0CA9" modified="2022-10-12" version="1">MQETFDQLEIEIENFYNQFPDSFEDEIIQNQNIQQISQLDNPQILNQSESQLEKEYIDVVRILNFEEVKVFIDELICPICSHILIDPRICLDCSQGFCKQCLSQWYNQYHSKTCPCCRSISYQPNDGSQAPKLLFKLLSKLKISCKYYNNGCRESICYDNKEKHYYNECEYRLIICQYCLGKIIKINSKTHFQECEFKPIQCTWCSQTFSYYDYQTHYGCCSFRTFKCTQCLLEYPFSQINNHTPDYCSKKQIKKFKNQYQQSKQEIQKTKQQLLEQMQQIKQLERFIKKQKLQNQENQYEIQNNEQINNSEEMNQFYEDIQLHIDPYEQQQQQFYWQRINIQEQYQDTQEKSVCSENYLEYPIQEVSFTFSDIGGTQHSQNSIQEEGYDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSF8</accession>
    <name>A0A8S1LSF8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0410087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="24124" checksum="CB6B9F1A40285B6F" modified="2022-10-12" version="1">MQVLRTADFFGVPFIQQIDPKQSIYKSAFGGLVTLLICSASLAYAIWVLYLWQNNQLSPKISNSIYVSDFSLLDLEYDVIKLYYWKFGENQIDPFESKILLPLVMYTTNFSFTELQLIQMSNETSVDGSIWHFRQQMENYIQHQKCIQKQFYVKRYIYSQMXFFSQLNDEDADLLSLSNKAKEVQLSVPDEICIEKDFYENNKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSK8</accession>
    <name>A0A8S1LSK8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CPL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0420192</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSK8"/>
    <dbReference type="OMA" id="DNYCQKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012959">
        <property type="entry name" value="CPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040059">
        <property type="entry name" value="PUM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389:SF0">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08144">
        <property type="entry name" value="CPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="78"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="CPL" evidence="4">
        <location>
            <begin position="349"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8068503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="537" mass="63486" checksum="8309CFBB12E7389D" modified="2022-10-12" version="1">MGKKRIVEENQNEEPEEVQEVIAKADNKKQKKIAKNQNKISKDVDFISFLKEDIRFLIQHRNKENEEKQQKAGEVYNKIEGILYQIAQTKIGGRTIQLVIKCADQNIRQQIFSKLLNDKQFGELLQSKYGHYIGITMVRNMVPEFRNQFFEILLKNANHYVAQGDASIVLDRFLTREATTQQINKVKQLFLNHSEKIDNLAMKIIEKGIHNHLLSLQILKVALPNLVPEERQKVIEYFQQLENLDYLRHKDGISIFCAILNVTDKKERKNFLKNIQTYMQQSQEHLHRNSQFYLALQKVIFTYDDTKQVNKSILQDILPEWLNSIHVFKIIQSIYQPKLRDDIKFDTIGLQNTVSLKDDEIRVKELQDYCFESILNSLQQSETNILLESNINQFICYFIQYIIKYSQIQALDFIKNQIKQLLYFKVDTIFSREVEQNGNYWILTQPEAQRIAKCLIQQQEIAQNEFKEIIDNYCQKIKSLLIQNLKVTLISKAIYLLLALIENTNLKEQVLQDVKKNKKWIDKLEKTQSLQILYKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LU41</accession>
    <name>A0A8S1LU41_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0380251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8066064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LU41"/>
    <dbReference type="OMA" id="NIMYGYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035921425" evidence="1">
        <location>
            <begin position="18"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="384" mass="43763" checksum="C8ABAAEB31ABD95D" modified="2022-10-12" version="1">MKIILTLILIYRNAINGYLVACNTLSQINCQNSGYCQWISSSCRSLECHKVTEVRACSGKLLGNLYQKQLCKVAQNIDPKFLNLCIGYKQDTIKWGYLVYPQDNTQKSTYVASSGQLISLIATKKYPGQFKSEILTLNILKAQANDLINIMYGYLQQTNELCDREIPISFLEKAIYESMQQIRDDETITFTSSTPTKTDYYLALWGMTDAFFLKLRSQKISQYISYKYLINFGFANLQMEKLCLDTQFEVFELTWQYQQSGYIEVLIMPGEQFGFDLIHSNVIIIRISDLDGQNMSSQIQLKWIHQSQKIFISKQIIQIDITKMSIINNISGTCTQLGCVASLILNERKAYIIGDSTIKNCVGRSQSACRLGECTWNDSTGICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZM0</accession>
    <name>A0A8S1LZM0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0410295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8068044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZM0"/>
    <dbReference type="OMA" id="VDYISIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="227" mass="26645" checksum="5E5CFC5D171E030F" modified="2022-10-12" version="1">MNSSLIMKEEVQIYQKHNFFFYFIRTEHKKLHEKSIQIQPLSFSFNNNNQDFLSIYEITSKQIDQYISINDVQINYSQDGLSNAIVCAYIIIKGFDLESAFQVMDFLQIDRPTVYYLSSLKRLDNQINSVQNLNRSSIILKTDLDLDQTIQNSLLQSEILNFPIFSQRLIETQRNSQILPSEYQATSEIFNQQQISFISFGQQDLQNINEEPQINKIQSSIFVNKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M2U4</accession>
    <name>A0A8S1M2U4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0520007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8073959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M2U4"/>
    <dbReference type="OMA" id="TIWIIRF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="30028" checksum="6DB2E5B690F04777" modified="2022-10-12" version="1">MLYLLYWRKSSNRQIDQKKNKVIPALEQPHLQLHRSQSVEQTIKKDFRRKMNRLGTQTAETVNESSNKIQKSTLKTTSQMFSSKYQIIKVSSNLVTLDISQQQEQFKSIPSMQELPETVRTQDTNLLTIKESEEQRTEFLNSRIIFKLINRLIVLQGFLGFLLFNNLHFALASLLSLITIWIIRFSQTANVYIDVLQLILFISFDFTSLFLFNPNEIWTYTYLVLLAIEVILEFIILNTQKQKNFENLQASAQQHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M325</accession>
    <name>A0A8S1M325_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0490004</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M325"/>
    <dbReference type="OMA" id="HEDASKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13431">
        <property type="entry name" value="TPR_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="332"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="366"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="400"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="434"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="468"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="502"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8072203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="68019" checksum="EFA82EA995A7311E" modified="2022-10-12" version="1">MDLSNLDFTIPNKDSNVLQQQQIKIAITELKNNTLSMINNLQDEYHHLKITIEHITQLLSDNIISLNEIISSLENNVLEDNLLSQIVECYNYEKNQSLISQENKFGQGLFKAYELLNALENLNNNQLYCLEHKKYSNILQKEIELLNNKKWEEAHIILEKRIIQIKNILSYAQFFNSTLSIYLGMSLMELNQPGKGIKLKEKSLKNNGNLIEDISKETDQFLEYNNSNKFINLFIKCLTLEDQNNFEQAIQISDQILQLDQLNIQALYKKSKFLVNLEQTQEAIPLIDQVLQINPNYVLAINLKGVILEKQRCFEESIQYFDKAFNIDNSFVTALFNKGGCLQDLQNNKEAIKCYDFLIKINPKNYLAHYNRSITLVDLQRYEEALTSYDQTIFLNPNYLSAYYNKGIVLNTLKRNKEAILCFDKVISVEPNHAKSYLNKGYSLSDLGQFNEALEHYERAIQIDPLFLEAYYNKGILFDDLKKYEDAIRCYDEVIKLDPNDSQSYYNKAISLQNLKRDEEAILGYDMAIKLNPSYDNAYNNKGLLLEKLGKKKQAIQNYKAAIKKCIKDQTIFSKRIKRLKNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3B1</accession>
    <name>A0A8S1M3B1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0520108</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3B1"/>
    <dbReference type="OMA" id="MESQIYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="113"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8074159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="933" mass="109594" checksum="4B150AAEB670A2B7" modified="2022-10-12" version="1">MKIYIQSPVFQKFIFNNQQQRNKLQMPVSQDKIEKPSPMSRSLTIKQRRQTQNTVETRGLSLSISRETQRSFFKEPIQNKVIPTDQFQTMIKEIQVGNSIQVITKLEQGPFSKKTYLILGIAYKQLNQFDKARSWFNKCLSEYPNYLDGYIQSAVLEFKAQKYYESLKLIEQALLHNPNDKQALQYKARCHSALKQHQKALEIYEKLNNTDFIGRSRHCLSLIKCNYFDQALIKIDTILQQDSENAEALLLKGIIYFKKKNFMESQIYIEQAIQMSTSHQLSAKAISYIIKSKICLMDFYEAQLHLSRADQLQRNFTCINKLKPFVNGTIKLMKRQFAQGLQLIRELKSISKFLLPIYYKFLGYAQFCTSDYVSALKSYEMINQIERGQLYNKYVAEALILVQKCQYEPSIKLLDKAIEIFPNKLEPYYYKCSICLLDKLYDGVQECEQLLELAFNKQGKQPNLFFARAIMKSYKRRYTSAMKDLEKAIDKSEDSISDHFYIRGILHAQSMRLKEAIKDFTTAIHFNKEHSESYLERAKCYQIKGDTAQSFGDMNKYVKYNNSDEIHYWSGSLLFHNNAYEEALKAFNDAKQTNQILILKLKCEIKLQQIDKACIISNQLTQQNVKEPKYKLDFQMLTYIKTILDTIQNKKQCDSVYFPNIDQKDIGLIFQSQDVLLFKSSSMIFQHKYNEALIILQQLENYWMKSQKDRMNSISSHQDADSEKSEDLLNEMVLKSERIVQHIIGIKYNMAIIYLLLSQYTNAKMKVEDIREQVGNVVSDELDYLKLLIQNQIDKKQTHVFNKSILTFLMNEKSIFNGFPTIKIHLYGQGIFECKLSLNFPDIQPPNICPEFKKEFLFMIKPNLIENKPEAPWLQRTSQQILVFTQNQLNDDLDLTTDDEVETNEKRKKKRQVQNLEIDEKVQKRLDQLMKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8G6</accession>
    <name>A0A8S1M8G6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0520005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8073955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8G6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="103" mass="12570" checksum="84D59C52AFED53BB" modified="2022-10-12" version="1">MRCYCGLQIRCDPFDDFYNHISNCKQFLSKSIFYKEFEKPNFYIFKLPQLYTLKAEFLFIINQIDEIINKKEINQTNSLKRNSRQLKYRIKLQFNQIIISNPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MA35</accession>
    <name>A0A8S1MA35_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0510038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MA35"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="288" mass="33284" checksum="632E51EB4F1CFC65" modified="2022-10-12" version="1">MATLFQCDGANCIEAFLEVAISTTASIMAINGFRIIFRDRNKLLSKLNKIIYGISMSQLILLSTYFIFWGSDFVISTIRCLRILNEILLCSLLAEIGFEKDFLEKLEIFLKVLSGFVFVEWFWTAIISNEEYDYYCLKMDLVYLSGTTVILTLLACGFGFYALDQLYISKQELKPTDQNKMDEQALEIKIFLGCDLLSGLIQFGWDYWANMSAYTIADCKSYYEAYSITSIFVIFIMKVLTLMISPMAIYYILYYKQRLQFNYRAANVLDINVLIDRRSELVVELTNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MET6</accession>
    <name>A0A8S1MET6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pirin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0610143</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pirin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MET6"/>
    <dbReference type="OMA" id="TPWHPHR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="2">
        <location>
            <begin position="22"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="3">
        <location>
            <begin position="170"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8079140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="33253" checksum="654D99C6F6BC8477" modified="2022-10-12" version="1">MSRRVLKIFTPPTVPEGQGAQVIRIIGNNHLRSLNPFMMLDHAFVKLPAGFPDHPHRGFETVSYLLKGSFYHEDSKGFSGHLQPGDIQWMTAGKGVMHAEMPGSWDELTSGFQLWINLQAKFKMVEPQYQEIKKDLIPEAKQNGVLVRVIAGEALGVKGPIYTRTPAFFLDVKLDENSEFLQRIPLKWNSICYVYEGQGQFGGQNANQHQVVHLDINDKEEVLKVKTGTQKCHFIMIAGEPIDEPFVQHGPFVMNTKEEIQQAFQDYYEQKNGFEGAHSWKSKIQNLSKKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MF24</accession>
    <name>A0A8S1MF24_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0570158</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MF24"/>
    <dbReference type="OMA" id="SCDSPRC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000227">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF375">
        <property type="entry name" value="PEPTIDASE A1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="10">
        <location>
            <begin position="34"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8076991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="503" mass="57971" checksum="8F0D1CAAEC2C5E4C" modified="2022-10-12" version="1">MFIIFILQLLYVLGQDDDPIHTSVLYGSSDLGYYYVNIYVGNPPQKQSVIIDTASSITAFPCDACDQESQCGNHVDQYYNRTNSSTYKELDCNSSFGECSCLKCFNKQCIFSISYSEGSYLEGFYVNDQVIFGDLLMEAKTSQNESISKKDRSVRSVFGCTTEETYLFKTQLVNGILGLSPKTNTTLAFPSIVDDIYNQENGMKLFFSICIGRINGYMTIGQYDFSRHQKNSTYYTINYFPSQNHPFYVVKISEIRVNNKTILPGSDLQQGEGTFICSGCTIIYAHPHVTRELVNVFVCDSPNCPNMEYFEDTACYIYDQTLHGSYEQFISFFPTYEFVMDNNFILDWTPKDYLTKSIKQDDAYCLPVQKYYGNRKVFLGQVWMRNWDIGFDKENQTLTFVRSNCSSDNLKHNFTADDLFLEELNQSNITLKKRYPPPIDIDQEFAYESVRVIAVFIISIIIIILIFCIRRIYLNRKIQIVSKIPEDEDETQKTVQSKVFVAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MFG0</accession>
    <name>A0A8S1MFG0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0610011</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8078878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MFG0"/>
    <dbReference type="OMA" id="WHACTEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000005">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="7">
        <location>
            <begin position="108"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8078878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="303" mass="34658" checksum="1A1C31A8C26E5AA0" modified="2022-10-12" version="1">MSDIDQWIETLKNGENLKETDVKILCNKAKDILNNEDNVIRVEAPVTICGDIHGQFQDLMELFKVGGDVPETNYLFLGDFVDRGYNSVETFLLLLALKVRYPDQITLIRGNHESRQITQVYGFYDECLRKYSTLNVWKYCTEVFDYLALAAVVNDSIFCVHGGLSPYIKTIDEIRIINRKQEVPHEGVMCDLMWSDPDEIEGWSQSARGAGFVFGADVVKEFNRRNGISLICRAHQLAMEGFKLMFDNSLVTVWSAPNYCYRCGNVASILELDENLKKYYKLFEAAPTERSANSKKTIADYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MH00</accession>
    <name>A0A8S1MH00_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0570065</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MH00"/>
    <dbReference type="OMA" id="LIINTCR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="15"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="283"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8076805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="318" mass="36154" checksum="80C7CECEA844AD79" modified="2022-10-12" version="1">MIQQFQLKGHKSTYIIYDKILGKGAYGIVLLAQAINSGNQFAAKIINKKSLSPTDIVNLRNEINIQSKLSHANIVSMDDAFEDNEYLYLLLEYCNKGCLFTNIQLSGPLREEKAFEYFIQIVYAVQYLHSNKILHRDIKLSNLLLTKEDQIKLADFTWSTSFQMGFVTPQICGTIEYMPPEEMLHNDLPKNGQILIKNGISEECKQLIKLMLQVDTSKRPSAKEILSSSWVQRYQRNNRLTINTCRCYSRFKDNMTSPTKILQQGSPMVSPLRVAYGSPLRSPLASPLNSPTVKGKFDSKQGSINPVSKQRLGTQQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MK43</accession>
    <name>A0A8S1MK43_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0640121</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MK43"/>
    <dbReference type="OMA" id="MRREWFV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8080700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="20644" checksum="C0E0FB903DA048CA" modified="2022-10-12" version="1">MGIPLSTIFDSWFSKKEMRILMVGLDAAGKTTILYKLKLGEVVSSVPTIGFNVEKVEYKNISFTVWDIGGQDKLRLLWRHYFTGTQGIIFVIDSSDKERINVAKEELMRLMGDEELRDAALLILANKFDISQVTVDELISKLELQSLRREWFVQTTCAITGDGLYQGLDWLSKQFKKRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLD4</accession>
    <name>A0A8S1MLD4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0650125</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLD4"/>
    <dbReference type="OMA" id="RALDCIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641:SF19">
        <property type="entry name" value="NEPHROCYSTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_6G03870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="100"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="490"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="575"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="680"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="612"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8081248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="818" mass="93803" checksum="81BF18899B2E7186" modified="2022-10-12" version="1">MGNQCCGNQPINRDANEQAFAQAIPVRLQQKLKPESQRFHQVLISSGENDKKDIFSNERQQDFILNLKPYSHLATNDSVVVSIRVVCHDYSYKDELDWYIDESVIIDPNCFETSLLRVIDKKTGNKYTLRVINFNDYEDYEFKQALYQIYIMQLIGNKCKNLIYLHDCYILENEDKEYSKGSIILLMEYCDSTLHEIISFRKFHNWQWTSDEIRHVFGELAQALGQLEQLNITHRDLRPHNIWYCSQSKTYKIGGFEEAKLVKKPQTLHNQLSLNNLGINIQQENEELLNTIRGVPDYLPPEVQKYVDASGAQTVGRYNPFLADVYQLGLNILMMDQLEVNFESSELRNVVRKLNNQRNGSRGTSNDYHDVLKLMLVGDESSRLTPAELSLFTKSYLSEIPLNEDNLVESMKYKKKYAGIESTKVYQLIAVAYYDLFEWEKWLEKMEEILVTYENAKDDLHAAEVLFDIANGFIDINNLTSAQDYFIKASILYNEIGHQLREQNKLNEALDNYERALDCIRKAHNGDDSYEGALLLENLANGYRLQGNLTRARSFLEEACRITEQEKGSDSLEFAKMSINLGKVHSLLGDYKHALKKVKKGLRVTEGHASGHQNLQQSMLSYNNQNQQLQNSALLLKAQGLTMLGEIQRLKGSLGKAKKNIEEALAIREKIQGSGNLEVASTIEVLGNVFFEMEDYHQAKSQYEKALVIKKSKLGDTHVEVALTLNNIGNSCKHLEEYDKSQKFFADALVILKQSQSGDDHPLVATTYGNLGILLKQIGNIQSAQQCLTKCIQILDKTLPKNHPDSLFYREQLQEIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQ88</accession>
    <name>A0A8S1MQ88_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0610254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQ88"/>
    <dbReference type="OMA" id="ATEQFDC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="123" mass="14190" checksum="9B2229F3AB7EA5EA" modified="2022-10-12" version="1">MIATEQFDCLPIEEVSSMLGSIQVKEYPIFDVQKGVLTKKKVKYHNYDDEEIEIKFECEQSQYCTANEKIVKIPPKGLDFLRIRILVPSNCQEHQISLTVDAHDNHTGLIKQKIIFKLNVVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MRE0</accession>
    <name>A0A8S1MRE0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0610255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MRE0"/>
    <dbReference type="OMA" id="IMQECEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="178"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="348" mass="40436" checksum="11E6AD533E938CEA" modified="2022-10-12" version="1">MSIKSKIVTQVEDGQAEHLAKLLYPPYQTYFHQAKVAHSKQTLLSSLQQVGMNVQEFQDKPVQQIMQECEQKLKKTYTVKVVGIKDGELAVISKRFALPKKKSLKQISNKEDPIFQLSQQPYQIHSQFPNKQLKAVKKVKDPQDEEFKKRLLNWQAEQFEKMKGKPQAEDTSDEEQNQESQSLPQSSSLKNKKSLPRIKLQKIRRSDSPKNENSEKIGDTQEVYAVFHNQNQNQKMSYSRLLNHVNDKQKKNEKNNHSPPLTNQLVVVNINKKPPEPPKLGGIIMELDEEHENYQSDKKQHDEQQQHQTQQSSSLQITQFVLNDTDAMQKRLKKIQEKLHHSEPEGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MRF3</accession>
    <name>A0A8S1MRF3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0680146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MRF3"/>
    <dbReference type="OMA" id="WESYVGM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="63"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="804" mass="95200" checksum="C1C6C43AAC88C3E5" modified="2022-10-12" version="1">MGANQSQQQGQLEKKKLRKRDYLIGMCMSQLASKESNDNSKLRKSLSENMKLQSQDILTKMSEEEMPNNPIKILQYFLNFLTIRECFQLKLVNKKLKFMVEMSSNIYSNFLTQFYLRKLQLRCFVEYGLHQRFLSVYYSQLLSIETNQDESWIRIYLSFQRSIQQMRIIEEEIQKTFNIEYPLVDKILEIGRNPVMPIPLLTDDILKQCTTSWFQLELAQRTTDYTSVEKVPLLDELTDKIYQDCLSKFDFSNMKHTQLLFELRWVVIDNFLKDPTILDSDNIPLLLRFLLQIFNFIYQRCLFSRNVLMITKTEKNPAMFLNMYTILWESYVGMMWALNRALKKIFNKIDLIFEEYFTTQFPKITFTSALARLWSQIVIKGTRNSQMDSIENELFTSFESLLKQKRVVLQQFYMKDHCTNEQQLIGQRSNDDYFKYCPSAINCLLNKFTSNILDLSLHEQSINWIGHSNVKVGSLYGIILDIVISQANELYSKASQQLSTNYLVFKDYIIADSNFMQEILNQWTVQVCLLPRGIEYLYEKVKINLRQHVLSCQLNQYIVESTNHNFEDNILESKIIGNNFQQLQLNEPDDLTYRVERDDSFLENVICEILKEEKISQNEQDQSQIPLQSVIKQPSYYQYSSAKQQSIIEGDYNKIRMLSTFTSSTRVSQITQLTSVYSQQIMSSYKSNLNMNQIKDIKTKLEQNQWAIYLKDIYNIEIDNKMIIEKRNNQIQMRNNVRKIPQEIEEQFNSNIEFTKVWSLEDTMSLFETKIKINQQEGINQNRNFSEMKVGQGLLASYFIQQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MRT7</accession>
    <name>A0A8S1MRT7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0620225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MRT7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="133" mass="15704" checksum="047F84A760544572" modified="2022-10-12" version="1">MNQNQQLCFVQKIPQANFQQFPNNYGYNQNNESQSTKKKQMSSSPSSDLFFSSITKNYFQYNCHQPKAHPKEVQNFNINTSCAVYQKQGSENIKRQDYINTFKQLKENFKNKQTLREKTQSEKLKLINTQSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MW59</accession>
    <name>A0A8S1MW59_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphatidylinositol-4-phosphate 5-kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0720165</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MW59"/>
    <dbReference type="OMA" id="CKFFEDN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00139">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="63"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="99"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="PIPK" evidence="3">
        <location>
            <begin position="459"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8084598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="814" mass="95642" checksum="6EADE09C504D2E2D" modified="2022-10-12" version="1">MCCSRKQKTNTQREEINKLGKLTRLTETELIELKTRFARMSQGSNFVTKFQFRDNLGLLGMGTMLHLSDRIFHIMDDDKDGKIRFEDFALYFDKVSHGDAREKAEISFKLIDKNKVGSFTSAEFNDTMQGVINSWIAMTGQNLTSETKKQIDQRIKLIFSTMDVDKADRVTLQQYQDSVVADPALLEIFDFARRGVTLETIDFSIYQQQRYIQNIEKKIDQLLEYMKQDNKKQEKELPHFVQGTVEQEQKQRNSQVDNLHSPIVSLFKYESEETQNNNNYKNDLGINKFFASEQFPSINYNHCNELSIEHLKNDQNQDFSFYTQQKPKKQLKALFQQIPQNQIEGDNQDPKEEDQFYQTEKSLESENEKNQFNLFIDQMSTDQLKEKYKSMITCVEDISKEVQRLRELIDQNQIKQQKMELLDQINEQRLHTIINIPRKQQSINNQKPTKKPKISVSFGHENFNLVLNMMIGIQMAVSSINIADDYEVGPKDFKLKYYFELLPRRAQGDKSSFKVCQFFDYAPRVFNSIRTIYGIDNHQYLKSIGPESILQSLIKGDLSCLQELTSTGKSGSFFYYTADGQFTLKTIHHQEFRFLKQIMRNYYYHLKNYPETLIIKLFGMHKIGIKYQTMLREKVIYFVIMSNVFSTNQEINVRYDLKGSTYGRYTIDNDPTVARKDLNFLEDKEKNIKLNINKNKSMLFFNQLEKDCKFFEDNNIIDYSLLLGLHAKGNKQLDNESSVYSENPQFDNFSKMVTVDNQYTLYIGIIDILTNFSTKKKLEFLSKRVIYGPTISAIPPRDYAERFLKFLKDHLFAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MX59</accession>
    <name>A0A8S1MX59_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase S11 D-alanyl-D-alanine carboxypeptidase A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0730005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8084820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MX59"/>
    <dbReference type="OMA" id="HAISIKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.710.10:FF:000097">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581">
        <property type="entry name" value="D-ALANYL-D-ALANINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S11 D-alanyl-D-alanine carboxypeptidase A N-terminal" evidence="1">
        <location>
            <begin position="236"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S11 D-alanyl-D-alanine carboxypeptidase A N-terminal" evidence="1">
        <location>
            <begin position="356"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="497" mass="56431" checksum="01C640F37279701B" modified="2022-10-12" version="1">MNANPKDAFSVSLSKFHRKSLHSQMANTSGCNQLNDLPNIPLPELKVRGLQSAKIKASPKRSPHILKFYVHDAQCILNDPIKKANRRYENQSEYVFVDTYLDALHVIAQEEKKYKQGNGQTIQSEEFSEQQISINPQPTLPNLPAQLMRERMRVYAQSQQKEPQSIESQLAQRQPKKVIKTATSLMTKKILPNKLTVSLSVHQAIPSIKHKVSAKRWVMFTKQKNEKCFNKSVGWQEFQKGEVASITKIMTCYTTLTYLKEYNIEPDQIKIKIPGHAECIDGTSAFLNAGGILTIKQLLFALMLPSGNDAALVLSFTIAYLMILQKTEIYQYNRYLKGAIIDIEPQIERNKKLLKHTFLDQMNKHAISIKMENTNYSSVHGLNDEKNVSCPNDISKLIEKCIQLEVFLEIITTKIFKTHALTDKGGKATLYKWKNTNKLLKKSGWMGVKTGVTPNAGPCFTGYYKNNDMEAIIVVLNCSSMNQRFRDAEVLLISALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N144</accession>
    <name>A0A8S1N144_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0740078</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N144"/>
    <dbReference type="OMA" id="YDSEARN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007941">
        <property type="entry name" value="DUF726"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920:SF3">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4 TMCO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05277">
        <property type="entry name" value="DUF726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8085322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="844" mass="101224" checksum="84E743D9088E0CF7" modified="2022-10-12" version="1">MNQLAKIQYILVVAQMVPKIVRTVSNKFRQYDDQIKVQQSYGDENIELSTIFQGDMTLEQHKQTIFKHLDKIKNEVIEFLKNYNFQKIYCTEKIKQKYYDEKMNFCLINLKEIAQNKFSIFTYEIGTKNEIIWLNKALTKMNQILILYFQVKFKQLNYQEDFLLYFAEILKFIQKRIIKRVLKCLPQAYYNIQNLLQYVCENSISKDFQLENLEKQFQTNYNLQFYQKSITETYNRIIEIFNKDALEETLIKFLNQEDQQTRDKFRVSEVIVNQFHKIIRKRMRYKILNSLQNYKQRDDSILKDKITQLFLKQLNQVQFFGGIEDKQINQNIQLIIFSIFWIKLGFNKFKINFLIKELGKYHEYEDNDFQQIDIQDLELHQKIDFYDQMKNLEIQFNKAIEWYNQNVDEDTNLLVICQIYMILANIKFKNQKLYFGDYDSDARNILFIIIQNFNDLKFGYFNHEIVKICENLTHNLQMKLYEDINLKIQKEASQGYTQSELYKQRSQIIFNNEPYHFLLQDTSVSILQSPSVIGKDYHQKHKLFDFILKTKEPIDEFYKNQQLKLLKQKYQEINDNINQKKLCKEYKFINILEEPKLFHFQTLSKDDKNSNVITLLISGFLSKNEDKLSKWKPLLNNRYSGTLIALHWESLEILNIIKQISEWFTNKQNEIYDQIENNQFIETTKEATIVGKCLAHYLDEGHLFGNRQINILCHSLGTEILLQCIHELDRFSQKKLINDIILFGGVADIYELSKRKWNSVSGSVHNMYTNNDDVLKYLFKLNCNFKHPCGLNPVNFLYKKIYNYDVSDIVGGHSGYWEKLDIITNISDFNSDYKILVKDVKEIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1P2</accession>
    <name>A0A8S1N1P2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor beta propellor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0700159</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1P2"/>
    <dbReference type="OMA" id="KREAWPI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002671">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011387">
        <property type="entry name" value="TIF2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Translation initiation factor beta propellor-like" evidence="6">
        <location>
            <begin position="202"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="450"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8083636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="484" mass="56623" checksum="842CF5902B788392" modified="2022-10-12" version="1">MEQNIPHQLVIRNKNGVYLSNMLAKSDEEMQLINQDGNSQLIVLEDYILMFNKKTIKLHDYKQPVKELEFTNLQNIHLTESKQNLIIMDKLDQQSFQLQILKFPSLESKGINFNIKNFKREAWPILKFSSEDQYFYIIQNQKLHCYADNKCLFQTDIGNCEYFSVQPNTQKPLIVCYSLMDFNIKESFLKIVDINGKIKYEKKLHQTSDLEVIWSPNGQSLLINKGFHEDNTNQSYFGQNQVLFYDFPKNALRELPTYEGPIHHVQWSPSSKEFILLSGFMPSGAVMYDNTCKPLFEFGKDHKNKIIWGNGRFVMICGFGNLDGSIHIWDAKTLKQVSNNKYKSASLCYWSPCSRYYVCAKVTPRMNVDNQYSLFDYHGKEIINRKFLELYDVQFRPSNIKFEERPPTPTKQVQQEPPKKQFIAFADNPLAQQMRAMKAGEGARVLQVDETFGKQSNNNYPPGYDPPKEKKKRVRKPKQQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N8Y6</accession>
    <name>A0A8S1N8Y6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0830109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N8Y6"/>
    <dbReference type="OMA" id="HDNISQD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="80" mass="9429" checksum="F3706B02FF0DCA5B" modified="2022-10-12" version="1">MKERVSDIQNIFNNTMAIKQIKYREKLRQIQETDLMSSLNQFRKLTQQDLAQFQEGAKQIFKIIAPGFDVHDNISQDTIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NA11</accession>
    <name>A0A8S1NA11_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0840152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NA11"/>
    <dbReference type="OMA" id="CCGYKLK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="117" mass="13749" checksum="87648DDD718B9FE6" modified="2022-10-12" version="1">MASNNHTQFTYIKRRHFSKKRKQESKPRNYTYITSDAKQMFLNLFLFQSYKIHDAANKAGIKYSSAKTILFAHRKQYKQKLIRDKMAANSINSKCCGYKLKQSGQEYPIQVITRVGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBM1</accession>
    <name>A0A8S1NBM1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0780181</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBM1"/>
    <dbReference type="OMA" id="MEADPFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001800">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8087401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="210" mass="23696" checksum="B90F57271ADAC04C" modified="2022-10-12" version="1">MEADPFIKIIVVGEQGVGKTCLLNQYCYERFEANSPPTIGCDFTTKVTQTNGQTVRLQLWDIAGQEKYNAVSKLYVRGALGCLIVCDIKDPNSLEETLKWKSIIEENSDQNNIPIMLVQNKCDLVSDDEKTAYYQTLQGFAKTNKFFSCIQTSAKEGTGLKQLFTELIQEIIDKGLLVNQQKNFKQSTNVQLNSVQSQQNQQQKKDKSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEF8</accession>
    <name>A0A8S1NEF8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0780228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEF8"/>
    <dbReference type="OMA" id="THENFAK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13983">
        <property type="entry name" value="STKc_WNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000918">
        <property type="entry name" value="Kinase, Wnk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000427">
        <property type="entry name" value="Wnk protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="38"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="503" mass="58458" checksum="3654830AFEEE1F76" modified="2022-10-12" version="1">MENNQIMFMNQDDQVQIFSDEDDQISQIIEQDSTGRFCKYNEQIGQGAYKSVFRGYDNQSGCEVAWNVFQLNSVPENERRRVRQEISILSSLKHDNIIKFIHSWHNKSKKEIVFITEIINGGSLKNYLRRITRPKLKVIKNWCRQILFGLEYLHRQNIIHRDLKCENLLIDTNNNELKIGDLGLSIQLQQSFTTSVLGTPEFMAPEIYQEHYDTKVDIYAFGMCLLEMVTGVKPFCECKGGTGQVIKKVIEQQKPQSIDAIQNDKIKAIILECLKPPEERPSATQLLQTHFNLQVTDNDNLPVPINEQLLFQLRDDSKNSSILKCNLSNKIMLTIGSSKFETNSEISIHKQTQQQGLVKKNLIIETEDETEQIFNKYYKDYICLKNDESLQDLEQRQEKELQMLKQLHLQQKQEYLKKTSNNFNVKSSSQPFQSSGFLSLGSYQNFFELQQDSICPKQIIESPCLERNSNNRLLKLEMITDNISQQQSFNQSQQVDIQLEVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTI6</accession>
    <name>A0A8S1NTI6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1010167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTI6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="99" mass="11460" checksum="1C6D6BB876768B0C" modified="2022-10-12" version="1">MAQGKNSSDKYLIKFDGFLSHEKFYQRCAKTYAFAQNINTNIIMIKIIFPQSKEAACVKQKFSNINIKISGKFWLKINSNLWATIKIKTQKMGEFSDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTY0</accession>
    <name>A0A8S1NTY0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0940033</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTY0"/>
    <dbReference type="OMA" id="FNCKVDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="140"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="268"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8093741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="585" mass="69273" checksum="0E9D4BEB8A0B3076" modified="2022-10-12" version="1">MQSKTFRSFFDEQTIDSSFWQKSISQINIEKIEHQFMLFEKSQKTGFWVSKNYIIYDSKLMKLRPNKQTIHQINLNMSRIEKIKYQNDQEKESCNRKKYGIRIIRNQRCRELYSRSKEINQKIWEELKKQSLQITFKHDYDIQKMIGKGNFAKVYLCNKKSDKQQFAVKVFEKSKMINTETDRLALLKEISILRKLNYKGFIKLHEVYEDETHIYLVQEYLQGGELYQHIKKNQKLPENIVAGIIATLLKSLEYLHKKNIIHRDLKPENMILRKKGILDDLVIADFGLADFYDPLGNYMFQRCGTPGFVAPEILQDKLYDSKVDIYSVGCLMYLLLVGKSPFKGSTYDEIVMRNFNCKVDYQSIESIISIQCLQLLKLLLNRRPSQRPNPQEALKHEWFMLNLDNQRYRELNNNEEQDQLNKEINKSIINDVGSCGYMKYFSGIQTYDKKPEYSTPQICNVNKKQDATIYTPQYAMLQSIQEQFKDSEKKISIRNLEDEFGDIIFEKESPQSHKLPQYQLIGKLKKVLDQNHLSKQPSLIIKKQSQNQIKDIEQHLLSITQINQNQTQYKIVNKIMNNLKNLEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NU93</accession>
    <name>A0A8S1NU93_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0860108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NU93"/>
    <dbReference type="OMA" id="ETCINCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8090584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="279" mass="33321" checksum="762B74ED50B74306" modified="2022-10-12" version="1">MLLKIKQDIEQLFDKVQIFKKESRPIYPNLKDQIDEKINLIKQQFNNVDEKIIKNYCYKILELKQINNTMQDIKIALDITTIEGSMMLKKSHMLIQQESILSVTTLQKKIILKLNQITELNEINDVMYNGNEFEGICIFRLHQIYHNEVENLQQQFQQLKSFNNERLNRLYQSFCDIINDSKFIKIQQQISSYSDIINLIDEYRDQLCEIIKQKNDQSQIINRTKSLQNLTSVSQDYMDEDDDHYETKRGKYHNKLKQEEIKSKRKESSSETCINCLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NY54</accession>
    <name>A0A8S1NY54_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cache domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0960134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NY54"/>
    <dbReference type="OMA" id="AYQIVIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="66418" checksum="9805E2793D15FE0A" modified="2022-10-12" version="1">MKQQKPFATKYIGQLKLRTQLVIIVSIQVLIIVSYVLALNLIHHSLLINYLSDVSDQLFEENSKHILNNMMEEYQYHYNQVFNLNGNALVSFHRLYHISKNQVLLHPLDINPLYQMKYGGTTKIPDPLRIIKGYGNFDISYSFMCYSNLSSYNQPKTIEEAVGIKLQEQIQAAGQIFYQGNLINQSFLYSYIIKEKINSIYPCLNRDKTIYQYVAEKRDWFIELKKNYESKSPYDHYNYTFTKPYLLYTDKKIGLSMTLPIVDKDLKLIGGLASNFLGSQIVEMLKVNSFGFQMIYLVSDKGVMIMHPYKVTVEQLPLYIYNESITGFNLTDWEYIQNLNNDSSCPKFDQISSLLKCRFNSYYNQEMIIGTRDFPQFKMKLIMLLSSQEYLKFYDDFQSTLEQKLHETTSNYIIWLFVVFFILCFMIIVITQILFNPIENIKQQAIHLINQQRKKRQQISQFTEVLMSDEIQSLVQAYQIVINKLESLSLTKTSLCKQIEDIPYPTKQISITQSDVKQNIQLNRQFQILNFENLKDLCTDSQYEKAGISIIRNVLKQKNNKFLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NY89</accession>
    <name>A0A8S1NY89_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1010037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NY89"/>
    <dbReference type="OMA" id="GPESCSY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000026">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="8"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8096329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="140" mass="15623" checksum="3287EE4A30737B38" modified="2022-10-12" version="1">MQGAPEVPRRFRLLEELQNGEKGIGDGTVSYGLEDAEDMQMTNWNGTILGPYNTAFENRIYSLKITCGPQYPKVAPQVRFVTKINLPCVQANGQIDINKFSILKNWVSKNTLESILTGLKNEMSSAANKKLQQPPDGQTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P061</accession>
    <name>A0A8S1P061_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1020038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P061"/>
    <dbReference type="OMA" id="RCPITTQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052438">
        <property type="entry name" value="Chromatin_remod/trans_coact"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572">
        <property type="entry name" value="GLIOMA TUMOR SUPPRESSOR CANDIDATE REGION GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572:SF0">
        <property type="entry name" value="GLUTAMINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="458"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1184" mass="137877" checksum="348E2CC25D735AF1" modified="2022-10-12" version="1">MHSTQQMSKFSSTYSTNYNTNTRRYVSSAKTSPMTGPLQKLIETDNDNFSKRDKNPQNLLQEILQTNNITKIQKLISTFTQHDLNIDNLSQQELLNIILKVYQLNLYEEINFINILYEAYTDKQHKDVLKYLILHCHEQLIGKSGNTTRLLEIFQEMLTLNPDFTSFQLEYQLDSIPVSFSRIQLRCMFLLNQYDILRSLKGAVALFEQLEGFIFQQFKSNTIPTQSDISTLVIAYILLSEQLELSNRHGLNKQALEIIGKPIDCSIIDLKRKFIYNASKLSQKYLGQMIFDQINGLHRKMSPDLNRSQIVSQDTIIHQFLLDSLDMVYLKNYKDMFDIDRLSTNIITQPYVYTLMEKILPESKKKDKVLSILSDDQIRPNSGKQFQIKQIKSDLNSPNQSKFKQQNFNVLSNNNSQTQIPVITTNKPISNRPPLNQQLSSGSLHSSGKLNQFDSQEFEIIQNKLVVQQRELDELKQLYTLQLTNKKQEPENNQLAEQMKKKIDQLENNLFQIKNENSINKKEKELKQNEVIELKTQLQDLITKQSQLEKLLQSQSQQQQMIIYQQNLLQQQQQQQAAAQTTQSLNQQQSNSIIQTASMNIQIPPKEAQKHQNILNHSPDDDFIQMDDTTPYIYKQNQSYIIQLLEMLDINIAFAKMYTRYQSASQDENDQWNSDIQNIANYRVEATVVETRDEGKSILLKAFDEKNSLLCQENINLELLKGLITYVDFQEMLPTNQPSINTTHQFFKFLVLPYTAVVPDEITQEMKLQFWPKPYGLLNGLTLRIDFMDKNCLIYIHHIETDQFRISICDPISKDTLRLDLEMDYSTMDAFFKDAKSIKDQYAYFSKTTLLKMTEKTPKFGDDDVAEALQEKHFDKNKVKQIQQSISLNQTYMVENLIFKNPKEFLKYLKTIVIQFEQYLKSLGISFSNTLFGQKYFRCKSWNTGSKNQLQIVLDNQDQQNIQVCLQNCFETFGPNKTKIKAKGLTTLPYSSIQREFSVMFDKLQSEEKIVIFQQLLYSFNLNVFDKACEQSQEQFDKNPIIQNSYDCGSYKRMILYDNSKISVVTISVIGANRRMCGVKFSVFNIETTQEIGVYLPTSQGEWDLRSLDKQKIKSSVENVPFAEFFLTQILRCPITTQILFKKILKADSKNNQINSNEIKNRKAFIERIDNLITWQEVLAAAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P2V8</accession>
    <name>A0A8S1P2V8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ethanolaminephosphotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1030172</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P2V8"/>
    <dbReference type="OMA" id="VWQKPFL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000036">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF37">
        <property type="entry name" value="BB IN A BOXCAR, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8097315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="43449" checksum="FD0ED543D709D96F" modified="2022-10-12" version="1">MKYLNYDFIAPNHLHYLKSYRYKGTDQSLLYNYILSPLAEFCLRFVPMNVAPNVLTLMGLACIILPHILFFFVMGDNFAGFIPNWLLWLTALLHMLYMNFDNLDGKQARRTKNSSPLGMILDHNFDSMIILLQGTSMTTAMQFGNTIFSVILYIIPSVPFYIIAHEEYYTHEMNLPIINAAAEGTISVAVVFAATAYYGCDMWLQKLPWFFNYQINQFVMLMFIISVIITMPAVFLKIKKFTSIASLLKQLRYFFFFNTVILYSILFTQTNVIQDHVRAYMYTVGFTMSKAVGVVALNHVSNQNLPEYLNSIYIYIIILLNTISGQIFGKTIIDEGCLIQFAAIISFLIHIHFLYNMARQISEALNIKIFQINSTNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8H8</accession>
    <name>A0A8S1P8H8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1090117</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000890">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="36"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="148"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8099379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="228" mass="26234" checksum="63745E28C5684598" modified="2022-10-12" version="1">MIPHLHLVFKIHIYNQLLNPFKSPQTVXYISLISKRELTIQSLNNQNITPLNIFNLLSNFGNVLVIIYIKHKTSALVQFQKLSHAQNALDHLNNQIFFGQKLKIFYSNYQEIQFPLPFQSPQTVVFMPQQSQFRFSETKSISFNPPSQTLHLSNIKGKICEDESYIKELFRGIGNVQAIKFIHIDKQKHMALVRLSSLEEALNGAALLHGKEIMGRKINVSFTKSKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P901</accession>
    <name>A0A8S1P901_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1100090</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Assembles a suppression complex (suppresome) by tethering SIRT1 and MDM2 to regulate composite modifications of p53/TP53. Confers both deacetylation-mediated functional inactivation, by SIRT1, and ubiquitination-dependent degradation, by MDM2, of p53/TP53, promoting a proliferative and cell survival behaviors. May play a role in the regulation of spermatogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Acrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="LYITYDC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052472">
        <property type="entry name" value="MORN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511:SF1">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="481"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1004"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="964"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1004"/>
            <end position="1014"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8099687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1291" mass="150533" checksum="0A5DCD49E0402345" modified="2022-10-12" version="1">MNTNFEITTQTFKSLHEPLQPLPRSCLFTKLFKSRDNKVIKRKQFQTKKEWLTWKNSIIRELLNQRILSSKYPDKVLTILNYTVETSNHQRNLYITYDCGDEVEPLFQYLNNNTISFQEQAQIFEQILQITCILFKSQFEHTNLKPNNILYNKKQVLITDFGSARTHYQNFTKEYTAQAEKDWQNNLIFFYPQEYQKIMQEDNLDFRNEQWQEVDISNQNFRKSIDIWALSMMMIQVFENNQTLPKKLTDYYGLNEQLLNQKIENLKQYSAGIFSEVIYSLLIGKKEPEQVYQRFIKQKQMLNIVSPQIESTFTIQQIFDNDNIIDDDVIIPPQKPENYFLDKNQIKNLQGQFDDDASSVKSSNYQINIIEYTEIKPNNLKQPPTLLPPGTGKKIAKMPEIKKYDITATQNFNPLSEIKDNEKPEKGKKPTIKITEYDQAQQQDQNQVKPFQETTIPGIIYPPLISNVPFIFDRNKQPEISPLTQIKDPSNVPEPIIQPPPSGPQQSDFLGIINLQPFQEDKSPPHSPSSPSQDGNSSFQFFKPQNLDAFRDFTDDPIPPSTPKKDQQQQYQTQQNGNYNLLSLEDLDKPEPPPIPPPKVPIIIQPGNDQSIKTIQDPTNKIPPPPDIPITLIPIKPTNIGDVTGDPVTDIHEPNYVSPKQIKDIQNFKQQEQLVQVPIIDDPFQNNPLDRNISRIIQVGGINKITSPLQPILNPIDQKGQQQGKNPSSLDYIKEKLKEGVTTLKEEDQKDLLPQLQMIQRVLNKIQKHITDPKEILDLEFEKQQQIPQFINDVLLQNEKEQLILFKDVNDGVESIIQNQQACQLIQNNQIEKNLHLLNILNPVQLEQGKVGLQSNKILLAEIEKLIEAKKNLNRFIENPNFQNLLQKQQIDLLNKEELKIYKNLLEIMPKEKRQAEVEKRLKQIEQKKQDEEIQEKLIEFLENPSFDKLLPNINLKQLDDEKKQKYKEALETLLNKVQQRQKQAIQEQIDIINKDIKKIKPGDKRIEDKKLKPENNNNNNKPSINQNFQDAYLNVMKKLGDEVDGDLLSLNQYIREFQLTSVNYQLTDLLPNDCQKYQEYVTKRIPKFYDFQGSKYKGETLNDQPDGIGILRKNGVSSIYKGVFKQGKFYWGQVLEMNDQGQLTQYVGYYNQQHQVKHGKAKLRWYQPQKIGFCGGYHFNDYESYEGDFILGYIHGKGKKTYSDSSEYEGDWKKNKREGQGRYTLKKQGKKTITYEGQFLNDFQHGKDVKAIHHHQTGLDLVVQGEYRRGKPIGQHILIFNDIPQRQIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PAW2</accession>
    <name>A0A8S1PAW2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1100169</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PAW2"/>
    <dbReference type="OMA" id="WIAISRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="510"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8099843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="584" mass="67993" checksum="5A540DEBCA3E0A56" modified="2022-10-12" version="1">MIDGWQDYLNNVNFYTCGGSKYFGISTNNWIAISRIFLDLEPHSHIIVEAEFHIIGNQTSPTFQIDEQSISYYSVISSQDCGNSQPEYLKTFSITYSHNRRNVWTTILNESGFQILENICFPFCGDKLILEEYEDCDDGNEQTYDGCFQCKFKCIEDCNICEKGQCILKCQDGYEFVNNSCLSVCGDQIITKEENCDNGNLRLYNGQNCFDCSQGTCLECNYQNQLLISNQCKYQLNCGDGLLQESEDCDDGNYLAEDGCKDCLIQQNWICITITRDTQSQCSFVQAPILQKFKRLTLIRNYNFEISNIEKKNWNSNLYIIKDVGSNVSFGEFIISIEIQQLLEFRPVLKIIVNQTVSNIDDVILDVFEKSITLKYTKFLDEKQMDYSQNLKNLNKYMIFSLSGITGINLLFGSVDLFFEIMAISQFQQYLKYINQQYPQNMEIYFSVYDNYLASPGFHLFSSNFLVFQYQYCEGKFNAYNQNTNLIINLSSPILKFIIFLQVNSIISNYILYFVVSMAKMALYNWIFCLRYFNNMTSLSLYINPKVIFKCSQSFYNICLELIKLKRFMIFKDYKTNYFSMVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PE29</accession>
    <name>A0A8S1PE29_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1150056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PE29"/>
    <dbReference type="OMA" id="QAELMLI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="261" mass="30281" checksum="62308AC47D70D03D" modified="2022-10-12" version="1">MIEGNLGELIWGSVELTLNVLLLDRLRLPQKIPAQGVLRLSLIHEYNNYSDTKVRYVVASLLTPDLYKYFRNFQDPLQFIYGCFMFFDLLVISEIITNTDAIYAATLLLRLLRIINILYQIFKAAMTIKIKKTIQYIPHSITKPSQVSPPSSLITTKANSHDEDQDQEILKQPKKLLNPFLYQFNIRTSKSSVITITTNEITQLDSLLDHQLYFDKRTLLMIKLNLCLKAYHQNKIKKLPTSEILLKQIANYQSELMLMHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PM88</accession>
    <name>A0A8S1PM88_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1220061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PM88"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029375">
        <property type="entry name" value="CFAP141"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35818">
        <property type="entry name" value="C1ORF189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35818:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15104">
        <property type="entry name" value="CFAP141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="99" mass="12267" checksum="FA0B81734A7B26C4" modified="2022-10-12" version="1">MQQIKRNIQLNQQYSEAERYDQNLKSISRNTWWHESKSKYDKVNELKFMNKVYSKEVENAYQELKKRRNCMLKDLYEKEAREWEQELRAKGLAIYKNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMT0</accession>
    <name>A0A8S1PMT0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1240091</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMT0"/>
    <dbReference type="OMA" id="CDEYIDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="346"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8104607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="509" mass="59916" checksum="D67A10029D9D4CD4" modified="2022-10-12" version="1">MKQTLPVLAHFEKSSDEIAKYFALKYGNNYHIIKQLQNQRKQIETPMLEAKRTLRTIEQECDGLKRSNSKQSERMKATSTFNFSRPGSQFDTFIQSTQHFDLNSGQLLFQNIKQIMESLQNKEDPPNFQLYWESVYMFKWEQYIYCFVRPLTQKIIQESIFLSDIVISFIQCQMKENLLNNNSQEHQQEMIVLTKLLLENFCNFLKKILTQTKQQNGLVIQLQTKIDLILCKNNPCISFIQELKKNNSFIKNIIEKLLQNDNPNIQPVITTCRYQLSQLKSLDIQSSRPQLVNAIYLSTSVNQQKEQYQSPLKTLFKIPHQQQYVMHLRESYSNLEPVEVPFIKTPFHHSLCVILDLDETLGHYNDKIGKFIPRPGLIELLQGIKEYCEIIIFTAGLQSYADHAIAELQCDEYIDYRLYRPHTTFNGNNFVKDISKVGRPLERTIIVDNTPTNYEQQQDNGLLVSTWIDQEQDRELFELKDLIIRIAQAKTKDVRKALKKYRDYISRHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQB0</accession>
    <name>A0A8S1PQB0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1250128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQB0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="147" mass="17273" checksum="5404DC5C66FB3165" modified="2022-10-12" version="1">MRQKGSQQFILNKQEQMVSSQQMDSRSISEYQNVQRESQSDLKITQCPRIVKQKGKKYMKIKFNTKQQLYSMVQRQGMKIKDAAQILGIKYATAKTIIFHQRQKRKAKRKCGERMCGYTKIVGIRVSRLKIICIIANEIVHQLDYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUH0</accession>
    <name>A0A8S1PUH0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1310121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PUH0"/>
    <dbReference type="OMA" id="NMNEGMP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="154"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="216"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8106702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="619" mass="73679" checksum="DD9CC445DE5742F6" modified="2022-10-12" version="1">MLNSSFNFFEENDISKLRKYIISKENEWKKDKAVLEQKTYLLELQLEDYKLRETNQRKLNDTITQAISDSTANQKKSYAELQKNIDIQQKDQSKIKFKEQIKQLEHQVRSLLEQLQEKESQGKEMELQYQKQYMLYDHRISQLEQEKLVNYQDNQRLRDNLIKQEDVYKQKEQQQKMIYDQELQKAKDLCIQDHNTKQQDYELKFAQLSTLHEKEKEQLQLRISKCQNTIKKYQQHLEQNIDVVNLKQQYEDQIKQLNNQNTEIQAQFQYEKQILLKQIEEIKKMNRDSNLIENMNCYNSTSKKRSIDAIKIKSSLQSYDSPVQCKSFHIACPESKIQIYNNYLKNSSTQSIILAQKQQIQSQLFQNNQSFDKSSNMNEGMPISIDQFNLMKDKKQKSQSTFSIPTSNNYNLNSYLQEQSIFQQNQESFQLDSKNNLKEETQKPKILKQLNPNISNRQIQFDYNRSIKHELQKQKESNYQRSMSQEGVKQQICNLNKMLGQTEISYQQSTSTQQITNNTSLNSFKVPAFTQNQLNTLKNSSQYMCPSTTLRMKENIQKIRYNHNHSTHEETKQNKENHQPLKNNQKSDIKLIIGRLLQNKGKQSDQLENTGYIMKNNRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q6E4</accession>
    <name>A0A8S1Q6E4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1440010</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q6E4"/>
    <dbReference type="OMA" id="ECINKWM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8110361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="580" mass="69244" checksum="6ED260A441BB046F" modified="2022-10-12" version="1">MKIKQIGKQLEKRGEIKEALNQWNQKILENLEDIRFYEQKTAILMRNDEYEEIIKCWDFGIENNKNNILFYKAKSIKQIYKVNTLKKLGRDIEIIECINKWMHLNNNCSLVDDGIPQISSYKFEIVNKCDEQDWIIKKKLLCSSSIREYIEYTNILKKLGKDIEIIQCFDWGIMNNIDNIDFYYAKSMYLESQGKNQEAIDCWKFGIQKNQKCVKFYESYSILLTKLNRIQEVVDCMQFGIEQNPNQITFYAKKVNALIELGEQQQVINCLDYGISQNKQNINFYDYKCRILKHLGRNQDCIECRELACELDNLDRVEEQLECWQRGIEIKKDCISFYQHKFNILWKLKRYEEALQLWDYGIQNNNQNEKFYYQKAQTLEMLERNEEMLECWDKGIQINPNNVEFYKEKCYALMKFEKFEEADESWVSGREDSNNSHNQLSDNEENSPLKLSENAEKALKCWDLGLSNNPHILEFYNEKIRLLKRLGRIKEALTCREQGIENNENDPEFYMMLAEDLKHEKNIIESINQIDQGLKKCDDSRLYATKCESLTKLKSLRKALIYFRLSDSGRYLGRKLKYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q6H4</accession>
    <name>A0A8S1Q6H4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1460129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8111162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q6H4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="56" mass="6758" checksum="8B5B23CC4A51E14B" modified="2022-10-12" version="1">MEEIQENHDKTNTNIQLEQLIFQQKREKLKLLNKIKLIGKIVENVKFLGRIIQKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7P9</accession>
    <name>A0A8S1Q7P9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Armadillo-type fold</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1480030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7P9"/>
    <dbReference type="OMA" id="DNELQYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001611">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8111542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="58421" checksum="3DAEE1B7EAE0BF8F" modified="2022-10-12" version="1">MDEIKLVESDDELQYQPQTLKNQKQSQPKSVQEMREQFRLQIRKEKIEEQLEKKRMWFIPKQRLQSSQLNFSINNLFNEFKIVYAQKEMNEISQKLHLIANIIDDRNRIHSLYLTQRNFLQFKDLLNDESYYIDTYTIYLQLTQDDEYFQLPIEDIQWSIERSLDLLIENKQIDDLANLILGYLGNVSSKLQQIQNYLLNKNTIDILCQLMKDSLLHNVCYLIQTICSNIPEQYKPQLIVILKHLKINKSQEILQTLFIINKQCDQYDKEINYFEIFQLLFIYLNEYLINENDFESFYSALLQMKYLSDFQQLYSIFQQFNILDIINKLINYKYQKIRTAVLYILQNLCKQGHNVIQQIFSQQELIKKLINILTTDKPINQSLVIRSIQHILKYGNYQDFTVLINLGLAIHLKSTIESAEPYIVLQGLECITSLMNYGLSLQTQLNVPNPILAILQQNQLIQAIEDLQYHPNKEIFVQSQKLITEFFRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8S0</accession>
    <name>A0A8S1Q8S0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1490127</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ICLIESG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23810">
        <property type="entry name" value="UBCc_BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000002">
        <property type="entry name" value="SUMO-activating enzyme subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2660:FF:000005">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000146">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF4">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="1095"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="846"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="427"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="2139"/>
            <end position="2183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="846"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8111962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2388" mass="279714" checksum="0F431FCDB3D19708" modified="2022-10-12" version="1">MSQDEYEDYEEYYCDREQQIEEEEYIEPQLQKVESTTQTQQYVQIVDPNKKEDPEFLTLLQENNIPQLEDILLSDFSMFFERQAKLFNYCANIIQFTLNSQKIENDQVEQLSKILKLFFQIQEIGLQSQFSQQQSTQNLGYSAQKIGYSINSDQQIVLTLLKLILKEDDQLQSIRVYLPRFLIFAINPPQPFTCATSPRIFQTIIVNRINDELFNNSFEKPQKLDILALSYFSQQTQNISIYKEIITILSSIFESLLDQKSSQNLNCQLINLLQFFLAFTTNQTILNIFFNNKFHRILYNSIRFSQPIFGSLNPPQWSKEPQIIDAMVNLIVRIVSIDQVDKIFIDILQDDFTLLLEKQKFDYIYRVILPILNSEQVRLIPVCFHQKLSVQECKQQVEKVLNSQKKNILSSSQLVTDILTKKQSEKINQLVQDHLSTQNQQKQQENEQLQQKQQINENPYRIKGQWVNLHQFNLQNPHLDSYILLNILDNRNNVLFIFDLMAGNENIRLACFINNIIQDKKQHTYIQAKSNYQSFYVMMIQDDHFWYVNSLDKKPHVILDIQQSMLQISHHNQPIACMDFINFQSSKFGFPFEKIQQRMLLIDQITASMIDQDCSLIQIEIWTFDESKDMLLSTDIQELIDHKSYTQQLNYYLSQFRFQPIHFISHNLTIQELSEQLKEKINIEEFKENEINDILVNKSIQQINLKNSSQKPLLNYFQTYIGGIEKILEQINKHLYTVKADQVYKQLFIELHFLSQIDGFYYSIIQTNGFLQLLLAVAFLNDKKEGKEAYNKVIQLMYESLATLLDQSPDLKEQALVINKIQIRIVNRIDSYLNLLNRNIQSQFQPNFESNKSNNPKLLDNQKGKPKGIGYGDIGYKTQIFDFQKIQEKQQQQDQPKKQIEIQQNQQEINLETLMRCLRILLDQNYKEYQVIECLQQSNILNFLIKYFQNINITQIEGSKAFNEILKIIENFAKHQVTLELFLINDNFSLFRQLETFNQWAIMFIKTFNSKIEEVNKFMEVYNYVESCLYASNMIKIKYLNQDQQSGYQQQISMHQFYKPFMKKLAVGKCEIKSDPEYLSVMKDYYQNNNNPNQVKMQKLIQEISNIEDNLPMESTNSIFLRYDQDRMDCMRTIIFGSSGTPYAHGAFLYDMYFGDDYPQRPPKMKLSTTGHGKVRFNPNLYNCGKVCLSLLGTWGDSWIVNFSTILQILISVQSMVMWENVMFNEPGWESQMGTPNGEQANRGYCNFIKVQNIRYAMIEQLQKPPKGFEEIIHKSFYLRKDLIKKEIESWIEQANLPATYGQTQNQCNYGSTPGLYKQELIKVYDELKVELEKLKFNIGTDFYTITDEKKKSISINQEFINKIEQQDINNMEMPQGLDMNNIDISYEDNLQQRQFDSNDQNLQNLMSRYIGVVGLDAVKKQSESTIFIHNLNGLGIEIAKNIVLSGVKRLIIFDPTIVQLSDLGTNFYLNNEDINYRRDERVLNKLKYLNPYVKIDVLQNNIQELNLNEIQVFITQDPKLSIEISNQNKVAVILAQTRNVFARIVTDFGNEFNIIDKDGEQLSEVMIESIQNNLVILFKNQNHNLNENDIILIQEVRQKEGQMESYNQKFQIRNVKRNSFELITDKIFCEYISHGVVYQQKQTYKLTFQRIQNIQNSLSNFCENMGMLDRIGEIKRGLIHFCLNNTNQLNDDWNLDKIKLFINEIQQQEIDKKLNQYFNEDVFNNYKNELMPLMILLSINTQFQPLCAIIGGMTAQEAMKAINRKYTPIYQVYVQSFEDVLPFKLRELNNLYSEYKQFLQKYGINQNVNSRYKDLINTIGCLQNIHQSNVFVVGAGAIGCELLKNYALLGVGKNGAIYVTDPDIIENSNLSRQFLFREKHIRKPKSLTAAAVVKSMNPDINIIARLDKVSQETQDIYHNQFYKQMNCVTNALDNVQARLYIDSKCVENDICLIESGTLGTKGHVQTIIPNLTESYASKQDPEQNNDIPYCTLRMFPENNIHCLEWARDKFEQYFYRKPSALVQLMQEVSPQQQTVDLALRILKKYPKSFQQCLELGRQKFQKLFVFDIQALLNAYPLDSLTKEGKLFWSPPKRAPQVIEFKGAFAYKFVEYYAILTAQIYGIEIPQQYDLNQINIEILSKQQIKKNKIQDLVEKQQNNQIEQEEEVKNYNQLLEEARNLLNQIKPNIPQPQQFEKDDDLNHHVSFITSATNGRALNYGIQQVDWMWTKLKAGRIIPAMATTTSCIAGLQTIELIKILKKGNNYRNTFMNLAIPFIMQSEPGEVEKKTLANGMEINIWSKYQLKIYKYLEPLSYIIRQLENLFNTQVISIQQGAKVIYVSQMLPKDEVELYKLLNTPISSYVQFVNRASQINVQLKGSSWVVVILTMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBR6</accession>
    <name>A0A8S1QBR6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1540035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBR6"/>
    <dbReference type="OMA" id="REQYIME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035798094" evidence="1">
        <location>
            <begin position="23"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="363" mass="40746" checksum="6BA00CD45C8A1893" modified="2022-10-12" version="1">MARIIPVLTLILAVVTARVAKSESPKEVLAQINKDSFGNSVLSVLQLQLATGGPVGEIQILLNNIASQLNGDQKKADKVHESDTVAFEKIIADLEQEIAYHQTQIVALSNLRDSTTEALGEAEAEVRVVTSDIANNEKSFADESATRQSQHETWVRKDAEHVDQMDAIDEASKIVQHLQAGVAFAQLKSRFEKVQAKLMESKHALFKPLINALTQLASKVDNKSIIKILELLAQIRQQLVASRASLLATEERQAANWEVQSGHLQEEHKRLVERKAFLENSIVQFKVTIQEAVEDLEDQTLFLEDAEDSLAIQERWAAEQESQYEAQTFEREQQLEVVERLQEVLTQKMSAASEFLEIREEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBV5</accession>
    <name>A0A8S1QBV5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Translation initiation factor 4E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1540007</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBV5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF8">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8113046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="18595" checksum="7246BC948DFBDE91" modified="2022-10-12" version="1">MKKTIYSKLSDILSEPQKCKIFKQFNDENADAQIEAIQFFKNDIKPWWEDENNKNGGEIQFDISTQYYAVYDSIYLDFLLQIIGQATEELRHINGLRVLDKINAKQGNRIRIELWMDIDPKDQDESIGRLIEWIDSTLKKHKIDIDQTQFNKVSHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCB7</accession>
    <name>A0A8S1QCB7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1520013</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCB7"/>
    <dbReference type="OMA" id="VCPITYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045862">
        <property type="term" value="P:positive regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="61"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="8">
        <location>
            <begin position="243"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="154"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8112531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="331" mass="38758" checksum="69C6E7A9B28AA519" modified="2022-10-12" version="1">MGNTTSNHPQITEVLTEYPFQHKNCRIASHSSQRKILKAASKQNELPHKSNRKFAPNIVKSLEFKSEGNHYIKIKQYKKAIESYTKAINLYNHDSIYFSNRSVANKLLNRFQEAKQDALQAILIDQYNSRAHFLYGTVILIEVQMFPDISEALIKQAQLGIEELEKASEQVKQNKNEQKNKLKVLINQNLAKGKRMIYLIRQEIDKRNILTLKQTLTDIAHRQHHHFDWNQIDQIIHKKIEIILPECFVCPITYEIMNEPTLINSGLTYDKHSIQQQFKLNGYFDPMTRENIDPLGLIQNIQLQQGIQQIQQQYGWVGIENEKDYKAIKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFR4</accession>
    <name>A0A8S1QFR4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1580013</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000163">
        <property type="protein sequence ID" value="CAD8114074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFR4"/>
    <dbReference type="OMA" id="EHESTWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF888">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="44"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="976"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="989"/>
            <end position="1008"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8114074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1078" mass="126250" checksum="78240C1E42F7146C" modified="2022-10-12" version="1">MFGETFETIEEQYNPTTITYQTRKAKNCADQMDMPELSRIKHFAGLKNQGATCYLNSLIQSYYMCPEFRKVILQLPLCKETIEDSANFAQNESRNRFLLEFQKLFIKLYSLQSKATSTEALTSSFGWNDGQQMFQQDVSDANKVLFETLDRSLYGTPFIIAPFYKGVVFHHITCLNCNNSHGNEEIMYDLNIQVEGNKNLSEGLFSYINPFLLDGTNQYFCDICGFKVDALKGDKIRKLPSILTITLNRYTFDYERMQRVKLNDRFEFPLEIEMGAYLENPIDNLIYELQGIIIHRGNAHGGHYFAYFRDLLDEGDWLSHIPEYWQEKVEYQQQKKKNEDEVDYDEVQLPFEISNPKVAQGWYDFNDNSVIPIPVNKIQKQYQGSESAYILIYRDRNLVPSKLSKEEIPKYLYDYVNKLNEKIEQDRQIYEEAKQHIIITVADASIIDLQNHKLLDEKYEKLTYKLRLSSTIQQLYEQINKDGYWLLEFQENYQNKLLHFPRYLKNLESTLEEEKVEHESTWILVKKDQDISQVGLQKVPYRLNLIINKQPQSFLLYISTTLLELKQLIFALSGIPENEQELRNVQNQKIISDLSDETQLGDMQLTIETQILVKKKMPIQNVNKNEKDLQVGDDIVSILVEQGDKVGVSKFYVNINQTVQELIEFIKEQFGIIDAHRLRNLNGSILFCKTDLPIQLKEFQNFREGGARLQIEKGEVPENGKISIVVQYKEQNIEIHVDPREDKIEQIKQEACKSFNLDSSQHRLYKVDWLQVASEGLTDETKTVSQCYLRDRDLLMLCDKTAQIDCELIRFQLYSTTTGYPDDCLFQKHETMRKDATLKDLKQMIISTFGLNIDIDHLRVRDINKNRFFGQVFRKLELPLQKLQFSTNDIVYQVLNNPENLKDDEMLLLIKQRDPINKVYIKPQEFIFKTSKTPTLNDLKEQIQNFIGIQEGNELQLAKFIIYEFQWLHINPNGLQKQQQGKNKGKQGKQGNQNNKQVQQTQKQQQQQNICDNLKKNPINLGEGDFIGYRIGNHPNDDFQTKEDNELRERMIAARKNKHNALGSIKRQQEQGFVVDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJ47</accession>
    <name>A0A8S1QJ47_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1620094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8115071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJ47"/>
    <dbReference type="OMA" id="HVPERFC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8115071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="30774" checksum="8FAECCD3EA897BD9" modified="2022-10-12" version="1">MYVPLNVSNDMSQPNFYQMNDYKQISNQPSSLHIIKSLTGIQMQEQYNSSTFNQGSQNSRILNLYATNFCGQLISQIPLFQCVSNPQSSTISSGQLKSLLLFVQKIEQPNSQQMPNSLNQNGLFLRLERKWQFSFLCFNRPRLNVFYVEKGQNKLLGYVEVPYTFITKSCYIFDGNNNFKYKITKRLLGQSKTYSPFPESNRYDLDYFDTKDSQDQVISQMRKQPKSEYYFDQQANFTIVFPEISSAEGKVLILAAALIFECMYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QN95</accession>
    <name>A0A8S1QN95_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinase domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1760002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000185">
        <property type="protein sequence ID" value="CAD8116611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="EMANCEK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050637">
        <property type="entry name" value="NLRP_innate_immun_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45690:SF4">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45690">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="689" mass="79868" checksum="A9A4AA67FB609C04" modified="2022-10-12" version="1">MSSLVKNQSSTKINFKKLIKKLIMKMNRELDKNQEEAAYHINRGALACFTTLCNTLSINNRVIEVNLSGCNINVYYAIEISGLIQKNTNIQILNLGSCLINSFGLSCIFKALIDHSGLKILNLFNNQAFTTAIVQDFCRYVLCGKNQLQEINLSHCNIQNSLLAQIMDKTHHLSSLRRLKLSLMCLNSEGIIALSSMMLNYTGKLNLDNLDISYNQIKNDGIELLAKGLFMSVRQIRIKSLNLNGNHISQTEFLEQIILKIQLEELHLAHNQIIEFQNQQVLNLKLLRVDLSYNRIEEIPNNFFLNVFYLNLSNNQINTEGAYQISQVLSSNKVKWMYLDLTNNIIKTKGFISIIYALIENTSLTSLSISKNQITGEGILVYIFNHEQIKLQYLDLSYNQLRYDLVYALISMMKESRLKSLILSQLFNDENEIQSGKQDCFEIKCTNLKELDFSYNPQIMLPILESLSKQYNRIEYLNLNACGLNNSILIEKLSILIEKTYTLTTLQIAQNHLGSLNQQSLEQLSSSFGKNSTLLNINLSNNKLKDSIQFLINGLQNCCSLRNLNIRNNLIDENXNRPIVGWVMLIRSFAQNNYFQIYEIQNSEFIFLEQVIINMNHQIRILIYHQINQMIFYQFMNIYPIMIKTQDLSLIFLQQILYQYIVVTFRLMFYSKINIVVFFEIFNQYNQNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPM6</accession>
    <name>A0A8S1QPM6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA ligase family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1810001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000190">
        <property type="protein sequence ID" value="CAD8116775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="1">
        <location>
            <begin position="414"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1286" mass="152272" checksum="98BE6F7A9C68FAF3" modified="2022-10-12" version="1">MSLFQNHSQFTICFNFNFATQRQGYEEYDGIIWDYNYNPPKEKKTKFRIKFTINQEIQYLREGQILRIDQIIDTSQQLKLLKNLEQIQNLQWFGDYGENNQKIGKWGVIWKGETLTNVGGWYSNYGKKEGLWKELTQNYWSKAQIYEVGEYTNDQRKQGWQYIYENKEIGGGEYNIQGEKNGKWIELSNLFWDFSQIFYQGIYKNGYKIGKWDTLYKWNDKFEQIGGGLYDEQNQQLCQVKIGKWIELSDKFNYNSQVIYNGLYNNGQKIGNWSYLYRKNDKFEQIGGGEYYQIEGRSMKNGIWIELNDDFKNDSLIIYKGDYINDKKIGRWNILYKSQEKEIFNQIGGGSYSQIDGNSFKNGKWIELNDGFKNDSLVIQNGEYKNNHKIGRWDILFKEDDKNKFKQIAGGSYDQVEGYSVKNGKWIELVDGFSKWSQVTYEGKYKNGKKVGRWDIFYKSFWNGKKEQIGGGLFDERDSIKIGRWIEESNTFYGQYLKGKKVGRWDIEFEGKQMQTLLLXFYQLYRDQIIDTSQQLKLLKNLEQIQNLQWFGDYGENNQKIGKWGVIWKGETLTNVGGWYSNYGKKEGLWKELTQNYWSKAQIYEVGEYTNDQRKQGWQYIYENKEIGGGEYNIQGEKNGKWIELSNLFWDFSQIFYQGIYKNGYKIGKWDTLYKWNDKFEQIGGGLYDEQNQQLCQVKIGKWIELSDKFNYNSQVIYNGLYNNGQKIGNWSYLYRKNDKFEQIGGGEYYQIEGRSMKNGIWIELNDDFKNDSLIIYKGDYINDKKIGRWNILYKSQEKEIFNQIGGGSYSQIDGNSFKNGKWIELNDGFKNDSLVIQNGEYKNNHKIGRWDILFKEDDKNKFKQIAGGSYDQVEGYSVKNGKWIELVDGFSKWSQVTYEGKYKNGKKVGRWDIFYKSFWNGKKEQIGGGLFDERDSIKIGRWIEESNTFQYDQQITYSGQYLKGKKVGRWDIEFEGKQIGGGLYVDQIKGSSIKIGKWIELDDEYSSGIQVTLQGEYKNGKKVGLWDIFYKGVAEKEFKLIGGGHYQEQKNGGSIKIGRWIQMSYGFWNESRVTFIGEYKNGNKIGRWEIWYQKNYDDKKNELIGGGSYDQEYFNKIGIWIEPKHKFRSNSQVTYKGEYKNGKKIGRWEIWYQKNYGDKKNQLIGGGLYDEKNSIKIGRWVEISDGFQDYSQVTYNGEYQQGKKVGRWDILYNDFWKGNNQQIGGGEYDEENSNKIGKWIELSDEFECRQQVIYNGEYLNGKKNGKWMKRDLRWNEKWQELKYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QUV2</accession>
    <name>A0A8S1QUV2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2320003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000241">
        <property type="protein sequence ID" value="CAD8118270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QUV2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="447" mass="53563" checksum="A519C014F4A052A4" modified="2022-10-12" version="1">MILFEAINTVTNYLKYGSDFKERQIFNKDWTKIEGRESYRISNLLLFQKATFSIQINEEGTVIYQKDGSILFIDYKDTLNQTKNLEQIKYLRWHGQYNNLQQKIGKWYALWKGENLEAGGYYCDQGLKTGEWIELFENFWDKSQVTFRGQYFDDIKQGKCGGGYYDQEAIKQGYWIELHKNFYNGRQIVYKGIYYNDAKEGQWDIFFRSYFDNRFKKIGGGVYNREGQKNGKWVEESENFWNGCQILEQGEYKNGLKIGIWESLLKEYKDDHYKLICLGCYDNDIKNGKWTEIHEKFMRVLKQEGGKLQINQLIMLLEEVISMNTDRKQVFGQKQKIMDNNQELIIFLDLYQRFHSKEDIQTEKKLENDKLIMIIRQCIIKLDYYFSEEEGAIIIEDQNLDYGLKQMIKFRSLIYYLFNKILESVICRKLLRRNQNRKLGNSNQQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QY92</accession>
    <name>A0A8S1QY92_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T3930002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000402">
        <property type="protein sequence ID" value="CAD8119974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="465"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="510"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8119974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="720" mass="85256" checksum="783E2D2414E384DC" modified="2022-10-12" version="1">MSFCPEHQQKVVFYGSAFSELKNRKLCQKCIVNMNKNLMLIEDLEKQFSLVKLQFMREIQTKRNKNLEVLHSIKIYLQKQRNQISDVMNIAITYIDTQVELIKKVKEESQETLQISNNEDFQTFSKLICQETSIYKNIQVSLLQYIKKVNYTQLQNESEKILNQINIDEQKFQENAFKDLYMKYNFNNESDINNQWICEEHKDKIYYVDLNQSNVVPSRVACMKCVPQYFTQYTTLDTLKKLWENRSLEIQSSINKNDKFINKQIGKAIESLLQVKDQICQLIDGCIEKINLIKQNQNWIFKENRFLLKKDWQYLDKKDIISIANILSNQKPEKTLIKEIESQCMQQLLFIQVDVESSYEKIQSIIRDQKSIFREYFHTDVNQQILQPSEQLIKSVQIEDSKEKEQITIGYQNLLNFELLQDNSYKEEQIYAIAFNEEVTTMIVGKSKLITVFKFDGKLTSIQGLNQHKDKVTCLHFMKKSSTFVSGAGDNLILIWELNNEQIWKLQQQLDGHSGAICCLVLSKNENLMITGATDKKIMIWKKLNQWIHFQTLFHHENEINSLSLNQAENQLISCARQENKIIISTKHSKDLLWLEARYIQTTQWGLSLSFINDSLFTFQPYNQKVLELYQRKEGSEEFLKSGQIELKKNSESMKVSSQIYIKSKSVFFHQNGSQIFIFRITDDFEFIIKSFEFQSDRXLNSYYLLLFLIYKRLQNIFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQX4</accession>
    <name>A0A8S1JQX4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8044983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQX4"/>
    <dbReference type="OMA" id="WIDTNER"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="522" mass="61728" checksum="911ABBEDAAC8900B" modified="2022-10-12" version="1">MHQQIQNIQVFEKSTYSNQDANLIELEWWNPNEMKFRDVQIHITITQENNIIYSSQEGIILRKEQIYDNPNKSEILHNIEQIQYLEWLGEYGKNKRKLGKWIATWNKSLLIVGGYYVDGLKQGLWTQPFTNYFSKAKVFESGQYFHDQKYGRWHYIYKNKNIGGGSYNAQGVKYGKWLEISEKFNDDSQLTYCGEYKNGKKVGIWAVLWNDDDSWYNEEYKNKLMGGGSYDEEGDGIKQGNWLEVSDRFDNHTQVTFKGEYKNGKKVGNWHIFHKQNGKGKSIMKIGGGLYKEEEIKQGIWVELSDRFYDFSQVKYNGQYKHGKKIGRWDIFYVKEKIGGGQYDEEGDETKLGFWAEVSDRFYCNSKVTYHGQYKNGKKIGMWNILFNWQGNKQMQQNIMQYICFSGGGLYDKRGDGIKQGYWIEIWNGFFYQSQVTYSGQYKNDKKVGRWDILNEGNQIGGGSYDEEGNEMKFGRWIELANDFWDFGQLTFNGEYQDGKKVGRWVEMDLYKDEKREIIYQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRZ0</accession>
    <name>A0A8S1JRZ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0040544</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRZ0"/>
    <dbReference type="OMA" id="WIHRERF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001534">
        <property type="term" value="C:radial spoke"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006802">
        <property type="entry name" value="Radial_spoke"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13159:SF0">
        <property type="entry name" value="RADIAL SPOKE HEAD 6 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13159">
        <property type="entry name" value="RADIAL SPOKEHEAD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04712">
        <property type="entry name" value="Radial_spoke"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="412"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="424"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8043320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="457" mass="52067" checksum="C229602517756BE9" modified="2022-10-12" version="1">MSKLSQVLKSHTDVNGVTLFDHINSFINEVLQSNHKDMNSIELLSDFTKKNRFLFKKLSSESEVNNLKDKIPEFADWVKRVSQLLKGQTVAQMPTILQDFVASARLLEAAGYGFGEEETYQIQLSIKQLSIETNATEMTFFGKILCRNSDYYVARGVVPKLPTDYLAPNAEPRGDGVNYYTFWVTNDILTDWNELPLVTPEQIKVAREIKYVCTGNLNQELKTYPPFPGKEKHYLKAQLVRILHSNLIAPKGLYQQKEDNPKELEFVEEFKIPEFAELQNPDSWVHVPAQILKQGRITLYTDGLSEEAKNALLEANPDLDSETTRLKGIAEDRPLEPFYQTNWLIKTVGDQMIYNPKDDNSGNNVYATVILKNYTWSGAVTVANQNQWAFFYVGYGFKDTQAAYVPIQNNDLQLEPEDKSEFPEPNPKNPPKPPEPEKVEGQEGQDGQEQQADPDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JU11</accession>
    <name>A0A8S1JU11_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0050490</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JU11"/>
    <dbReference type="OMA" id="CMRAKWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000178">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040724">
        <property type="entry name" value="PheRS_DBD1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003210">
        <property type="entry name" value="PRK04172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18552">
        <property type="entry name" value="PheRS_DBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="247"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8043836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="518" mass="59545" checksum="1E54D7BBBE344108" modified="2022-10-12" version="1">MDQKQDYANIILTQLESIETLNSLEIAKSLSINHQDLVGCLLQLESANYIKTEGQKQDQLILTNEGTTILNSQSGEYEIYQKIPQEGISMLDLQKLFGIEQVQKVKQEQGEKKEKQIEDPKKKQFDLGFGNAKKMGYIKMDKGLISKVANDLPDKIKLELSQLNTIKLGSQQAKLLIERKLAMISTIKYFEIKKGIKYANKFREQFAALTTEQLLNGEWKECDYKSINLNAEGEKISMGNLHPLLKVRKQFSQILIGLGFEEMPTNQFVESSFWNFDALFQPQKHPARDAHDTFFCSDPELSEEVDTNLRDKVKSMHQTGGVGSIGYQYEWSHEEARKNILRTHTTAVSSKMLYRIAQLYKEKGYFPKKYFSIDKVFRNETLDATHLAEFHQIEGIVIDKNASLGQLMGIIREFFRQIGINKLWFKPTYNPYTEPSMEIYGYHPVLKKKVEIGNSGVFRPEMLAPLGIPEDVNVIAWGLGLERPTMIYYDIRDIRTMVGPEVKVETVRQNAFCVFESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0H9</accession>
    <name>A0A8S1K0H9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0H9"/>
    <dbReference type="OMA" id="YIWPGET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="270" mass="32453" checksum="749748E5F4EEE851" modified="2022-10-12" version="1">MKIVNFKTLKSLFSKIITNRTIILYSSLFDTSLQCYQNENEDYNIILLQGIQTQIVILYLDQIFTNMTFQILKQNMKGNSKQSNSLIPFQGLIYYPFGIYLGLIQPTYLQAYDQMLNQKEQEDVKQEVKEEYDSESIKKEYESKSIDKDCKKNIVKFSLYIWPGETKNYYKNIGKKLSSFIINSFDQPQVKEDPFIIQFINMESQNYNRIHFQRLFHSKIARRIAAKFFGNFQWCSQFISQNKTDIDFYLRHNKQMYRRKKRQQQSQFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1Q6</accession>
    <name>A0A8S1K1Q6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0120432</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1Q6"/>
    <dbReference type="OMA" id="TEFCETD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001908">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="14"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="330"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8048362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="439" mass="51392" checksum="137C55C0BF2BDE69" modified="2022-10-12" version="1">MLNEKVIGEYVYLVSDECKVGVGQFSHVYKGYHEKTKKLVAIKQIDKKQTKGIFEQMLRNEISILKQLDHQYILKMDAYYETPNNYYIITEFCETDILQIIKQKGSLPENTVINYVIQISEALKYLNSKKIVHRDIKPSNILIHEGEVRLADFGFAIHQDKVGIEDRQFQIGSPLYMSPETLIKNEYNHRTDLWSLGVLFFEMIFGVVPFYSTEMDDLIRKLQQYQKDYRLIFKYPISEASTDAIQNLLAFNPEHRCDIKKLQIILKNHDKNRVFGDASMHLQKRTATPDKKRKVTPDQSPENRSKVQLAKKELVKVSCPAQFFDKKSTYFAKNKPNKEGDQQNSKREENLGTDRKDNGTAQEISIIRLHDQKPQNNEITQENMNNEEFLAFLIKKLESAKTQDTNQNKQINECQFLLYQYYGKDQNMLQSLKNKEMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1X2</accession>
    <name>A0A8S1K1X2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1X2"/>
    <dbReference type="OMA" id="KSIKHQP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="640" mass="73747" checksum="1705A18ACDDB764C" modified="2022-10-12" version="1">MSTKKQVHGGKTSRSNSKQSASNPTLVRKRFSSLCSPPNNPGKSPCVFCQKFKNQKVESYASLLNKALFAKYSSSQNYYYSKDINDIIDEESTPAVVFYRDLECIVEEEEYLKRSYAKKEATSKIKALLEYYKYHKDIPRLFMQKVYITINKFHEKKRRIEYANIKRRLNIPDEDIQTSKKKEKKKQKHSDEDLSVGQLKHLLKDLKLDTNSYLQKKVDISSSVQLREFVQQIGQKYDYLGQISGLLSIDQSNSFLLRSQDLSTINKNLKQNYLINHKPQQNQNSKVQILDSKTTQQISYQGSLDKINFNRLQFNNQGEVSSLKAENSIKVGEQTKRSHQEQSPLRNSQQSQTIQKVYSQIEGFANFVQKQQQSLQKQQKQNYGQKSNSQRKVSANFNSTDCQFRLPSRQSSTQKQSAELQSYQVNFLQIYSQQLLIEEPEQLPNYKNMMMIIRQPLATDRIQRSSVYNIGEKSQQKSSSKNIQKIPQKFTQYALATLRNLQVKNSEVKKKQTVNSNNMQAPTLNSQLLHYRTKSQDAIGTYKTSPPKQPIRQDNSKDPLRITNNICVQASSKKGGGVYNINQNNIVNIYIEDLKSSAKIKQGGSQTARINSPQKNNPKGIFEVYNTQRSNLSPESKKMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K763</accession>
    <name>A0A8S1K763_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0170405</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8051129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K763"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF50">
        <property type="entry name" value="BIFUNCTIONAL ALBAFLAVENONE MONOOXYGENASE_TERPENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8051129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="12674" checksum="CF92BAE0BEED8B09" modified="2022-10-12" version="1">MQLNIRLEFSEKHFVNPEDFDYKRWLTPEPILNNDNFIYIPFSAGPRNCIGQHMALMETKIILSQILKQFIIQANEHVIPKWTLRFVYQLQPSNCVRLIKIQEKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K875</accession>
    <name>A0A8S1K875_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0170315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K875"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="98" mass="11485" checksum="E3F627648463E015" modified="2022-10-12" version="1">MIRISTCKGDQSIDQDNEINWCFEQLPTEISAKRHENKIRTDKNNNEINGQNKKFSITFRDEIFPKEGLTDVQIVENWKIYNVNKEKAVADDCLCYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB76</accession>
    <name>A0A8S1KB76_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0180359</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB76"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8051841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="36" mass="4231" checksum="FB50C8A86BA58858" modified="2022-10-12" version="1">MFIQKFNVNFKSLNTLELQNVKFIGLFCYNINKSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDJ7</accession>
    <name>A0A8S1KDJ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0190225</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDJ7"/>
    <dbReference type="OMA" id="YQGLEFN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="221"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="326"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="406"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8052523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="455" mass="54282" checksum="D7B858A4B95A5C71" modified="2022-10-12" version="1">MFSTKNQNHRKTASSAKSNETQKKSSNQYQSRYTNIQIFQQLTCLKMLKKLVLGTSQSLNKNNSKTRIFTEGNEKQLKLHTSASPIKNNNYQQSSSPPRRNTSSQVKAITKLLNKNINHTEYKRVTRDQRNQSADFQQLLAQQQLRQENYLLKNNSKKKFNSYGNLNDQVYFSQKGDLMECQSTVQLIQNNFEQKLINSKGSFSMKHFPSPMNICTQQITLQEFKDKLNKFSKDLKQNNNNIFEELQKTQKKQQQYRYDKYDYQESEKQIRQEISQRSKSVDSQSSRSRLEILKYSKDYLNQKKIKQQQNIKEEEFIINTQNYINQKKLENKIDQLVFQMQILKKKSEELELQNRFLFENLTQFQKESDCNAEERNLLMNKLDQMIGMQKRQEENLNFFKQIFVKENDQSRRIKTEQQQQQQLQEDNIQLNQRTEILSKQRPFVKSAYQGLEFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KFL9</accession>
    <name>A0A8S1KFL9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="ORF">PPRIM_AZ9-3.1.T0190251</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="31">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KFL9"/>
    <dbReference type="OMA" id="TLHCHEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000199">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000695">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="27" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="29">
        <location>
            <begin position="133"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="30">
        <location>
            <begin position="431"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="30">
        <location>
            <begin position="469"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="30">
        <location>
            <begin position="505"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="30">
        <location>
            <begin position="542"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="27">
        <location>
            <begin position="45"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="27">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="EMBL" id="CAD8052575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="571" mass="65962" checksum="599D417E5CBC1767" modified="2022-10-12" version="1">MGNVCCRDDSRQQRDELDTSPNPEASKPPEQKALLSQQGSKNAQIEEIQALIENQKCEYSEVHQEPEQLQAVVMKEEQYKNPEIIQSQKITNEKIKNELMKSSQEADSKSKKRIKLGFDIFVKEKEGSIGQHYIFGKVLGQGAFGKVWKVTHKTTGLVRAIKQIKKSSLIKEEEQRLFSEMNILRNLDHPHIVKLFELYQDENNYYLVTEYLSGGELFDRIKKMSSFSESIAADYIRQILLATLHCHEQNIVHRDLKPENIIFISEDPQSQLKVIDFGTSRKFDNQKVMSKRLGTPYYIAPEVLGHSYTEKCDIWSCGVILYILLCGYPPFVGKTENQILDRVKLGKFTFDPEDWETVSKEAKEFITKLLRMDPNKRLSAKQALDDPWLAKYAPSTQVNKKALNNLRQFQAETILKQALMSYMITQMSTQKELQDLQEEFQRLDENNDGFLSKEELLKGYLQIQTDHKLVEDEVERILQIIDINQSGQIDFSEFCMAAMNQEKLLSVQRVEQAFKIFDQNGDGFISKKELEAVMGDLGDDVWNQILTDCDNNGDGQISYEEFVKMLKNKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KNT1</accession>
    <name>A0A8S1KNT1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytosolic endo-beta-N-acetylglucosaminidase TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0250125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KNT1"/>
    <dbReference type="OMA" id="INASINM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033925">
        <property type="term" value="F:mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000355">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032979">
        <property type="entry name" value="ENGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005201">
        <property type="entry name" value="TIM_ENGase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246:SF1">
        <property type="entry name" value="CYTOSOLIC ENDO-BETA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246">
        <property type="entry name" value="ENDO BETA N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03644">
        <property type="entry name" value="Glyco_hydro_85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosolic endo-beta-N-acetylglucosaminidase TIM barrel" evidence="1">
        <location>
            <begin position="251"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="960" mass="111681" checksum="B0B24BECCFB9122C" modified="2022-10-12" version="1">MSNYFLNSGLKRDGQLKLVPEHVRFNLTQLTPSDVGIIQFQLILAEQEQNIIELIDIHDNLGNPLIKITLKQRELLIQYLDNTYKFEIQQIILYKLYPIFISFHDEISLITNFSIQSQKLQQRFTYTSNKVGIFLGSKSNLNAFTGITRNLQIRFVRENELKMQMEGEIKIQKSILNDLNDIGQIKYKLPFSKPLQSIEELDNWKPNEEYTILPIQQLKKITRTTNQPLLVCHDMQGGYKEDIWCFGNPMRQNSYRFYYMTHCDIFVYFSHTFITIPSVPYINICHQFGTRILGTIITEGNDNTLTNQILNLKYVDKLVQICQFYKFDGYLLNIETNVENVDLFIQFIKELSDKLQQIGAILIYYDSHNAEGNLKWQSELNQDNMKYFQACNYFFSDYHWNLNKLSNTEKNVGNQRNNVFVGIDIWGRGQYGGGQFDSYIALQEIKRAQLATAIFGQAWTYETSNDSRSQFIQNDNSLWQGKNTVSLFDKHKNQWAITFNGGNGWKIIQENGEETAISSFDWCVRTYTIQLNLVSSNKGGIIQFNAKVKGTGPKFDDLYLIGIELYDRNNRCIASIDSFSTKVNKREIQFNVDSVNIADDKWREHTLSIKTTDDTQYVKFIEVGKDVEYWAGNYGTQFTGESMVYISYDSNKDLLRMLKPKTYQEFPLQTYFNNAVGEAYYIDGQKNSHYKGYYDNLNDFDYTLCYPTKKIISQDIQDNWESSINFSDSWNGSSCIKIKGDLQVHKSFLVKLYKTRIDPQGNINASINMKYFDKKILQLSLVMKFADKGLQIFKPTKILTNKGWDIAQYIILNQGKSLKSIYLKIENISQQQQAVDGLIGGLAIYDDKYTEQMKSANVDFKDPTKYIKNIQLKESITNLYDLYIQLELGELGPLLKTIRIFNDKQWIGNVRSNYAYFPELPFDGKEMFVRLQIVLNNGNHIKAESVLPYILYSDQVVRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KPV4</accession>
    <name>A0A8S1KPV4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0240323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KPV4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052920">
        <property type="entry name" value="DNA-binding_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358">
        <property type="entry name" value="ALPHA/BETA-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358:SF4">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8056461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="35676" checksum="1AE071C06B141FEE" modified="2022-10-12" version="1">MISDLGPKIRYLQTKAIVRVDFVFESRNIQLNASYFLTKAQNHRCMIYLHGNASCRLEGIRYAEVLASWDINLCVLDFAACGMSQGECITMGVYESQDVIELMKYIEINFGKVDEFMLWGRSMGAVTALLLTENTKIRSYIIDSAFSEFRQLLQDIGNRQFGVFSFILYLAIPILRWKILNHAQFDINLLNPIDKLKQVIPNKKFFFVAGKSDTLISYQYTLKLYENCQMTKKLDICEGDHNSNRPSTTLQRIIEFINEACEFQNNQEYQIKIDHYFKFQFQQSLEEEEDVSEIKTPQTKQQFISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KS91</accession>
    <name>A0A8S1KS91_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KS91"/>
    <dbReference type="OMA" id="EVECFFP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="265"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="356"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="62566" checksum="A02F7AB1D65077F0" modified="2022-10-12" version="1">MQGQQQQKVKMQENLGQLCCTNKHQNTEIEYIIKDNSKRGSERLLCSKCIEFFNNPPNLVYITKAKERIELVKQLCYNQTEKVAEQFIQDIAKYIENLRTLTSDLQKQINNLINQCIQWIDNLNYYKTQKLQYKFLDEIDNLEMDEEELIQQSLAFDIYEINSINENNDGRVSLGIQNVQQTIKKFSNLQGMAYKIIKNANNYEHFQYKKMKSITYQVSFEKKEDQLCNALAFNSDESLLATAADKNIKLWKFYGGELLETNTNLQGHIDQVVCLVFSQQENLLISGSKDETIKLWKEISQNKWQPVQNIKIDSSYSLSLVLNQNENQFIAGCFDGSIKIISFNNDLQTVLENYRLDRHTKPVYSISLNSKGNQFVSSSSDKQIIIWEKNNSQLWEFKYVVDKSINDVCYRISYFGDETILFQQKKNGQTHFLKMQNSKFVERSDLRLVLNNQDPEVECFFPIIYNQKNQVLVLKHSQYVYIIKENKLQYQVACSPIDCLHPWNYGTMTKDGRFLIIWEYATKNFKVYLLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVI4</accession>
    <name>A0A8S1KVI4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0220368</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8055024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVI4"/>
    <dbReference type="OMA" id="DIMHEGE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13431">
        <property type="entry name" value="TPR_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="324"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="380"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="414"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="490"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="496"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8055024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="538" mass="62517" checksum="599042609C3298B9" modified="2022-10-12" version="1">MKQQNLFTCQDIAHEGEVIQGFCLNLGCQDSRSQFCLQCGFDPKKHTNCKKDLKGFGQIESFLTKFNQHILDLTIQLNKSYSSVKTKYEEFTKQLHNLKISLLNISEGLSQQDYKQIKDNLQIIKEWYQYSNNQEEIMKQNQIGTQLFSIKSMIQTLDLDNKQKQQIKGIYQNNDITLEQGIELLNQKKWQEAHEKINQYLKLLEKQQSLATFFQGISLIKMNQPGKGIINIKQAKKKINSNLYRDLLDYSDIELRLNPNNEYILIAKKKKYQLAIDECEKLLIEQSQYLHALYRKSLSLQNLNEHSQAIQCIDKALNYDSKYIVGYSTKGYSLDDLGKYNEAIAQYDKAIELDPNNANAYNNKAIVCYDKTIELNPNDLNAYNNKGRSLHNLQKYNEAIVQYDKAIKLDPNDANAYDNKGHSLLNLQKYNEAIVCYDKAIELQPNNAIAYNNKGQQFVYCNRSFITQFPKYNEAIVCFDKAIELDSNCAIAYDNKGSLLSDMKKYQQAIENYEQALLDCKQDQNKFKKLIIELRNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVR1</accession>
    <name>A0A8S1KVR1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVR1"/>
    <dbReference type="OMA" id="WNENEDL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="152" mass="18056" checksum="3003A419C0A76E55" modified="2022-10-12" version="1">MNLDQFQSTEEDWPQQSWNENEDLDFSSSPIMFGRKSHKIKKENDINNQDYYIQIQQIEEPSDDQEFNKFFINSSIPLSKKKIRKSKKINSSTINNLYQEIFSSILENSESQQETLKKLQQCQNIKVLIDGLERTLMNIKQMLLLKVKNNIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWV6</accession>
    <name>A0A8S1KWV6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0280315</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWV6"/>
    <dbReference type="OMA" id="FLTHIYR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14010">
        <property type="entry name" value="STKc_ULK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056981">
        <property type="entry name" value="HEAT_ULK4_RUNKEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044591">
        <property type="entry name" value="RUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46562">
        <property type="entry name" value="SERINE/THREONINE-KINASE ULK4-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46562:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ULK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23606">
        <property type="entry name" value="HEAT_ULK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="382"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="307"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8059267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1241" mass="143005" checksum="E2C7CFC1D137A41D" modified="2022-10-12" version="1">MNNYHIYDEIGKGKYSVVYKGRKKKTIEYMAVKSLEKSRRTKLLNEVKMFSGLKHPNILKFYHWYETRNHLWVILEYCPGGDLMALIEADQQGLQENIVLRFVRDMAAGLNYLHSKGIIYCDLRPSNVLFNEYGVAKLSDLGNAKRLVDMISATIGPDVEMSKRGSPYYMAPELFHEGGVYSFQSDLWALGCIAYELCIGQPPFQCSSFTELVELILNAEVKPLEVQCWDFIKGLLEKDPLKRWGWEKICKFLNIQYLNVPSQPHFETWCKKNNLVRVTPVQQNNKQVDIVRLSLNVHKNMLREDVPSSSQQQQYHKQISSNEKDFKLTNKDQEILIGSEQQQEDEEIDKQPEEFQTNVQSDLNSINSPHLQQKPFTIKKDSVHPSTQSLLNQSPIPGGITPQQKKGNENDQLTPIDQLFTHSSDNSVKPIIGNREIEKSIDATFAKESLPFQYINSDDVNRNIETDQIEFHFEQIYNSLQVAKQQEQLNILNYFEQIIQTSNAANRLINSAYVQLLLKLLTVSKLTQLKYRICCVLGLLIRHATVIEPEVSQHGIPEAMVEILNSKEQSTVRRKAAAALGEYLFYGATQMEEDPQNPYWKMTNLSFNQLIKVIKSQNEDEIVKFYCIKTIENISSQSISIGQRFAKSEIVLMILQCFLSTKNDSLRISCAITLANLLMLDNQQVDVFMQSLGLKNLVSIFLDNLQRVQQAMITIFNIHLLQSVSTQDSSSNNVSQLASNAVNKLLMEESQLIKGLVYLLDHGNAVSRGKAAIALMLMIKFNIVTLINLSEEQLRFYQILDKGLRDNNDYLKQCLQHLVQQLCDCTPIMITQINQSLTDSQLMEYSPKKTNGNYLNVLFQYSQTPVLQQSLFSSQYLPILTELLSLSKNKTQIQTTLLNIMSKMCTNSKILIKQQQVIIQQVLKNCIDYMVKQQSTETKFMFLKLIIDLLSALLQEEGIYDYPNFLKPYSNELNNIIITLILPLLKELFQEQPPLPFYALKLASILLTYNSLFLPHFKRHEVLQAILDMYKPDSNNITGHTLNIIQKIVEGSSIEDLKQFQIIDKSLQIFVQFTKQKQEWAFEDLSSIMVAITSKIINELQRGNNKIVVSPDNTLIPQPFPQEFLFFQDQFYFTLQYAQQIINIATPGCQLTLLTYMLHLTYFYPGKLSQIRQQLFNQLIPLLCPQLNKRIIKLLHWVVVLNEQKPFKIEKKEILIQVLDKYQKEDKSIAMAAKDLIKYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZH8</accession>
    <name>A0A8S1KZH8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0270309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZH8"/>
    <dbReference type="OMA" id="IKCRFYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039740">
        <property type="entry name" value="CNOT10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12979">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12979:SF5">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="322"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="719" mass="83997" checksum="B1C2EA54719DDDD9" modified="2022-10-12" version="1">MAQSFAILQNSFKHFGEGHYKESMEELQKFKQLKEQSDQNQSMVQINENIILCEYNITRDANACISKLDEIIDEIRKPQTQKKKKQDDDSILQYNKAIMLFLSGKIRQAHNLLKQLKDNYNLDIYLNIKVNLLLVETSFQLQEYVYASELYKKLSSEDTFKSLQQKNSKANQIDDQQQQSSQQIYTSLLIGSDLPYPDQHPNTFSKEEFQFLLNVIKFRFYMLSNSKDWKKQLINLEQSFKNYIIQLDQSNGMAQSMVPQFSQEIQPYLKLQAQMILKLMKAQKHLTLNENVQCIKMINPQSEIQNQQNLEFTKQKQFLYLAQIYNNLGCVHAKMGKYALAAVYFHKAISQTKLVQQQSNLYEGIIMTNVKQRLYAMYQNLADALFMDQQYQKALNIYNQLQDLCNQSAKFWYNRGVCFIQIYHDSMPDKNELYEISEDHQQLNAQDEGKKIILQSKEIYSDADEEVQDQFYKIDQKNEENKFVQSKANKEILNNAIKSFRNAIILSKKEKREEIIHLDQDQLQILGSQIFESSIVFLTYSLLCRGDYNLALSQGKEALEYNLSDNNKFIIIQYVLEAYIQISKVKDALNFMNSSSVSQFLNKFNNSNLQFQCRNTIGIQTTCIANYGPKAIYHFNLSVLHLHNNNITLAWNSIQAMMNCCEININSSNVAIPIPILNLLIWYYLKSDNVQLAIHLIKRRRLLTGQMGKNKISLLNITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L193</accession>
    <name>A0A8S1L193_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0320087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L193"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="232" mass="27445" checksum="8C1D67692648DFE2" modified="2022-10-12" version="1">MEPFAILTNIIKPEYLNYYRDWIYIASEKEARGLQLIGAIYKHKGRKLFRAKPPTVQLQELTDQQELDFKKAEEMHRKNQTSTTYGTEFGYLQKLLKPQNNVFKFKKCSELEFAQPLNDLAKLFLDNWIEMNDELEFQELVLNCLRALFSRFKAQLVPKTEMKVKYEYKPDWKLSNPIRVDKAGTDINAYKTNYNAIFKQRLKQEQIKQKIAELDGTDFAKALTIHKSFKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2E5</accession>
    <name>A0A8S1L2E5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0320142</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2E5"/>
    <dbReference type="OMA" id="QFRDLNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="426"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="543"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="577"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="636"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="777"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="674"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="106"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="268"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8061779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="850" mass="99701" checksum="E18C3B4C5DA78754" modified="2022-10-12" version="1">MSFFRSKKMKYYSLVIPRESAWVVMNELARLDQLHFVDYDPQLPMINRPFANYVKRCDDSLFKLSCLELLLKEFNKNLNYCENVDSLLDYYQQVQQDRMKPGHTYFDELEQEIEQKKIQIQEQSANLQNLLDRVNIITEQQLVLENAKEILGQSMFQQQVPHNPNDYQQLKFGQLIGVIDKEEEVRFKRIIFRVTKGNAWVQIKDLNNEQIDNSIRKSFHLNQNTTSQPRCLFVIVFPGAEESSSLKIKLLKVCDSFNKQRIEYPNSMDQVQRKMMELAQQLQESKSLIEMTKQQLEQSLDKLVLQQQGCNCSYFEYMRLYILKEKYLYVNLNYLTMRGSIFTGYFWLPEGMEIIVEEKLRNAMINNRDHYPTGQIQQIKPQPYTPPTYFNLNEVTMPFQEIVNTYGIPRYQEVNPGLFTIITFPFLFGVMFADIAHGFMLFLCGIYLIFWKNYLLKQTDSMFNLMIPFRYLIVLMGFFAFYNGFIYNDYLSISLNLFGSCYSPEKDEWKRESKDCVYPFGVDPVWQASGSSLNFMNSYKMKLSVILGVIHMLFGILMKGVNTLYFKNYLDFFCEFIPQLLFMICTFGWMDFIIIVKWLNTYENNTDPSIIETMINQVLKPFDKSVNPVFPNQAEFQLRITQILTLIAVICIPWMLLPKPLILSSKNDKHKVSMSDSQYQPLVSDKQVSEQDEDSNQEFDNDLQNVANLKSFSEQTQEEHDSGEIWVHQMIETIEFVLGGISNTASYLRLWALSLAHGQLAEVFYDMCLAGKLDIGGILGGLLGGYFYIVFALLTFGVLMTMDVMECFLHALRLHWVEFQNKFYKADGYLFNGYSYKKILNDYLKQSNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L436</accession>
    <name>A0A8S1L436_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cathepsin propeptide inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0290014</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="2">
        <location>
            <begin position="51"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="198" mass="23267" checksum="C879B7BDF26BE220" modified="2022-10-12" version="1">MPQKFFKKRLKFFFQMEKTFLAVGLMMLLGASFFLDNTLEVSDETNKATLFDNWKTMYNIQYPNKIDERYRLQVFIENLNYINAFNQSPEKKTYTLGLNQFADLSKEEFEEIYLKLRVPNSDNINPKDKNFKYTEGAIDWTDGSKIIYPDVKHQGKCGSCWAFXQMEIQCLGLICKNLIATEIKQTYESSKDSKISFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L918</accession>
    <name>A0A8S1L918_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0350131</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L918"/>
    <dbReference type="OMA" id="KSMYKHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001326">
        <property type="entry name" value="AP complex subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="7">
        <location>
            <begin position="11"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="609"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="639"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="639"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="661"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8063769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="680" mass="79486" checksum="1A058F40A4C97A19" modified="2022-10-12" version="1">MAYFGNNPQRGELQELYADLNDLNFEKKKEAVKKVIAYMTVGKDVSDLFQSVIKCLEFNDIEMKKLIYLYIVNYSRQKPDDAIMVIQNFRKDVRKSENPLVRALAIRTFGCLRVPKLNEYLIEPLKDCITDEDPYVRKTAVLCVPKVYEVSPELCPPLLELLQKLLEKESNALVLANLIQSMREIEIVSGKQIINLNQKIIQKLLLAVDECIEWGQIFILDYLATYNPQDSKQAEIIIERTLPRLSHINPTVTFCAVKVILKYLDFLENGDLVKNLCKKVAPSLISLLSWNQPEVQYTILRNISLILQKFPILFENEIKVFFCSFNEPYYIKYEKLDIMIRICDSKNFGQVLNEFLIYLNEADPHFVRKTIKSIGKIAITYDKAIDKAVSILIEFAKNVQQPTEPVQELLIQMQLIYKKNKQMYKHEDSLKIIYSIIDYANEPESKSACAWIIGEFGEFIPKSVEKMKEYIDNFQMEDRLVQLQLLTSAVQLYLKYPSQCSVLIQQLITSAKDSFNPDVRDRTYIYWRLLSTDPEIVKNLVCFNSGVFQNFSKDLRLWDTKDLVIALENMGSISNLFHKLPHQLYKNIKIKINNQQDIKIYKGEEKQETQTQVQQNENNKKDQQQQQQQQQQQQQQQQQQQQQQQQQQDIDLLSFDDPPVNNNNNNNNNINNKTDLFELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LC13</accession>
    <name>A0A8S1LC13_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0360192</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LC13"/>
    <dbReference type="OMA" id="DEMIQKT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8064569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="262" mass="30481" checksum="4B2936D671418D53" modified="2022-10-12" version="1">MQEDQSKKVDPMDAFRKKEDYKEACQKLDGLHSQNKENDETKYLMLFKKQETSRKDLCVIIRVYNGNMIWMQKIDRAALDEMIQKTDIKGTYSSFCELIRFAISENSYDFVQFEAQLDIVLYFSLQRHVQLKGQIPLDQGLPITNDEDTRKIQFQFVCDLVDVLKATQLQFTKEIEVNTIRKKKNEQMIDVEQPPLQKPPPPPKKNLLTIGQEILNTPEVLLPRKGPYGIPFYGNQYVQFPSNADLINPSKKKRKTNGGKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJB4</accession>
    <name>A0A8S1LJB4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0360305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJB4"/>
    <dbReference type="OMA" id="YPQANIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="857" mass="99959" checksum="7CF8F900C2C7843F" modified="2022-10-12" version="1">MKFQEIQREIYTNDELCLFLKFHLRISSAACLTLSIIQLYLSSQYNLQFHPFFDMLHLMTEMISILFAILGLVYSFSYITKLQQANCQYYENQNDNISRFLFIAICFTLTIVITLGVMNPIFYGIGMQSKVAQMEHSSYEDLYLLYQSMKSQSNLIIEVIVSAAQTISLIFMGYVFLLFSKNTITYNYFIYFFALIANLVAIIIYSNENDIKSISIINHVIKSNDKYLITYISGVSLFIALIIFSIFLIDTLGQYYRIFKNPIYKFILGNILIGFVFALIGSNGQLIRNLQQYQEIYLAKDECRNQMNLIPSEILEDLGCSKKYFGVQESFQLDCPNEQQTQIWEHKENEKFGCINWNCCSLISQILLKKYISYCLLTNWEAILLIMLSFMLFQIRLVQENAISQTLKQIALLFSFSLIFAGIFAANSLPDIEIYRKTYVSPLNYIPVNNLNTLQTHQMFQVIDWLPQQCSQKVPIEIIQNIKDYCSNQCKQIDLILSIQNGQFLVNKINQKDIIISQEDNVDQEFLYLQGNFKEIIEHIEDKIQVCLFSSSAAIKWNFEYSEQKGLHKHTKQLDSLLIHEQIMQIPSKVQQEALNQLIKYYNIQLVFYQNNFLPIGTQITIYNSLLYNCQSININAEKSILINESNIEKNELAFYNYPQGQYATIVFNTDNYQSCIYIQSNQNIYIHPIIPIQSDLTITLSWFGMYDLDLIVENKICKVGKKYQNECGTIKTHFDSLIGNQQTSTNLSNGEQITIDYLSTYDYKIYVQITLNKEEFIRLKKTQQDNINLYQQSNAQVQIFAKNSRYPQANIFIKQIVQATQTIKENDKFKYKWFVGCIKNGEVLNVINQIKINYEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LMT6</accession>
    <name>A0A8S1LMT6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0410090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LMT6"/>
    <dbReference type="OMA" id="FEKNCIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="226"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8067642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="385" mass="44954" checksum="832C5110D7AC82AC" modified="2022-10-12" version="1">MHPKNSTPNFNIEKQSVPQNSCPNRSVQNFSVYDDYQYKKVTTKLDSKDPMFHSQIFDQTETQKQQIIELQRQIKIKDLSYQQLATAFFQQQQKFEELNNAFAKIDEEKKYLQKEIQNLVEKNEFNLFDMKQLQEINTLFQNENTQLKLAAVKLKDTIVKLQIDNKQLKDQIESLMTNPLSCDRKGSSRLSFDYLLNEAEQTQTAQGISGSSLPNFEKNCICQKKIIHIQSEIKKYQKQLQEKDQLIDKLTQQNIALQRAQMKIQKCQKKQNLFGTPETEIAKHDPILVQDDSNDTSRQLNTYLSDKRFNNVNNGLESQMPLKTSSSARSVLMNKSKKEVEQIYKKMNQSALFTASLFSTMLDYKNLHQNCQQIRSTSYNQVKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSU9</accession>
    <name>A0A8S1LSU9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0430255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSU9"/>
    <dbReference type="OMA" id="NKVHERT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="126"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="274"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8069215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="42594" checksum="9979ECDAD0A39B8B" modified="2022-10-12" version="1">MTLSESLFQLISQFIELLNDLDLETLNVEQILNFNIMIIIFISFFVAIAEQTTFYGTTLNIFKNYINVQQTATSFLNKVHERTEQHEEIFKKFLSILERIDDLYDQQEKLLIFNDANIEYVLKKTRKNYNDKINQISANIDKNSEQLEKLEQNLQKNNTASLKIIEELKNLSSLIQEVLKDNFSINYQKVIPFLNEKIQSQYFSQLQKLKTLDYQLLVKELEKYSSFIQQQTIKESKKRDLANCYSLINSVLKQALILLLSEKQQQQYEVSIKALIIREDNKQLTQQLQQIKRLKRLFEDSQEFKITLMKQYRENIKFVQFKKKQFREQARKIIEVNEEKTKMFINEENILNQYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTH0</accession>
    <name>A0A8S1LTH0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0470120</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTH0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="392"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="397"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8071320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="425" mass="51388" checksum="C08A8D06C58F5C5D" modified="2022-10-12" version="1">MQQQSSPKIIDIAINTEMLGRYAYYKIQVDQNIISRRFNDFVHFYDALLTNYPGLFVPRIPEKQVMGNLEEDFLRVRRRMLQYFLNSIFKRKILWNSPETKQFFNEQNQTWLPLNNVSLSLQEKYKQNAPDCSNIEITLDMKYEYYDFQSFVVKVKPMIENFKKMCNNYVTAKTNFNTEKLIFVNYVLPEFEKNLLYKSDKKFFEKIKPLDLMKNQKYDKIRKFQSDQILIDLYIMESDIESYLISFEQIRKWEKKIIQQEDKIRQFQLDLQQTINNQRNPVEKLFFRSKDKEISKLQNQIYQYSKSIEELKDLINITIGQICLIEIPDFIKEKQYNFFKLIKFVGQQEIEQISITADYWHSILDSRLISQIEQQLIKDSVIIIEQKLEDQSQQLNEEEKSQNQENKDKKSNMILQQQKQQSEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTW5</accession>
    <name>A0A8S1LTW5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTW5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="54" mass="6749" checksum="416630C91E5B2879" modified="2022-10-12" version="1">MQKVDSQQNFTLYNTYESSEIFDYSKNYKFRVHLEYSLYWSAQIRIRGTWKQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYK5</accession>
    <name>A0A8S1LYK5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYK5"/>
    <dbReference type="OMA" id="GFIDYLC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="757" mass="90721" checksum="9A1541B0F4A0D60B" modified="2022-10-12" version="1">MILKIRQLFSTITEPATGGRQWLKFFQNSTGIQINIERSLKNEATENLNYYVRQNYKTFNQEQLFQAYIKKDLQFEIQKQVQMEVLERFVADQMNFSENMLITYLNKEAQLLPAFYSVIAQNLLLNGLKIFDQLNIYQQIEYMRLCQQAGFLHPDIFQKFIQNLKIDDQFIESLSLLQFRNIITLFDYCATSPYDLGLQPGFIDYLCNKYIQKLSNFELVASEITKYEFSQLMINHHFSFLSEEQYSEIKIAYAKILERMFNKNYNFVVHRNLNTKDVAKKLLWIYKPDDFKLIQKSLNHLISNTIQQGEFKADHMIYALPLYFKYFTPQLYYLLLRQCHKQNLNFKISKYDLKQIMYLLNSNALKGFRSIMADHTILDDVDNFIKNTLTSYVNNIKDEELLNFEEIFNQNMEQEAELDFNDAEYAEILPYFQNNNKNQETQNHVNQRQFIVNLTQKFKSLQNTLYKNGFQRISNQKINKRVSILEPILSYIEVLYYVSIYNPEFQPQTKLFKTPQILKLVNNLIYLPNLLENLIQINQIWPNLFNEELKSIIIANKTKLDQVYNSCQDCQEFIDKVEGQIKLTEQGKSRKLQYRNKNYQGLLQAFNFARPKPHNKLSPILKNPFEEYTIEETYSNQDFNMYWKLTQRSENVIQHTHFKELMWQALKLDKNQWIMNYSDFPHIVDFACTKQKVVVFLDEYPRLIRGLHKVYTIETERAAEYFSRLGYQVVFIDYNVYHPDNEQMLNILKQKFVFRYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M065</accession>
    <name>A0A8S1M065_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0500119</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M065"/>
    <dbReference type="OMA" id="AININFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="420"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="341"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8072929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="491" mass="56364" checksum="688A6DDD27C8CEA1" modified="2022-10-12" version="1">MQQTTEEDVQRSKAKSELKSIFLLVSIIMGFCFLFNTNITFGWDYKIIIVYTCTTQFAFMISKLLSYKGYLPMNLKISVNSYCCFALLESALLITYLLGIVYNNEVSAININFISYALLILQFVILAITVLISRQSVESDAQDQSKQIQEILVAAIKSVGSLQIVFLSLKLSQQINWCWMQTLVIIWFILGTMTLIEVCLILDLILKCCNNQLQNRKQLIFGGVWVNIVIIGFIIDIGLTFLGLGLFLDYNIGQVNLYGFITSIVFYIFQIFFYFKNQEELILFLNLSEAQQQTRPSEQPNEQITLKERIKEIHRISISKVPQFMVKLSATYFRKQEINENQKNNKNELGSSQDKKFRSTSYSDIKKQKEIKKSPSQDLDEDLTKKFDQLLSSPRIKLEVSESMAPELSSRGQYKQNLLCLVCYEKESNMINQPCGHGGFCQECSQQLLSKSNYCMLCRKPVTHTLLVQGVENRESLVEVVGIYQGGQKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M484</accession>
    <name>A0A8S1M484_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-dependent RNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0490241</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RdRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.48" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HYEDENQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007855">
        <property type="entry name" value="RDRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057596">
        <property type="entry name" value="RDRP_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079:SF55">
        <property type="entry name" value="RNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05183">
        <property type="entry name" value="RdRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RDRP core" evidence="2">
        <location>
            <begin position="506"/>
            <end position="1093"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8072663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1343" mass="157494" checksum="E10DAB69BF5590D4" modified="2022-10-12" version="1">MQYRLKNVEKSGASKLSALEIATQFENTISQKFGNVKLLRIDINEQQESIITSVYENKDPNNMYNKFLDVQFIKEILQVEELEQDEEDDDYEDFDNGVVLDYKYQQLKQAIDCFFEFSKDLDQVKQMQLENKFKSIIGNTTNKEQKLQIKLLIQCKGNQVLYLHQEKLFFNNFDKDPAYIDLISMNPFVNPDVSNMMNSTLIKVASFQLGMLQFIQEQQQLQLQQFFTSAQFQKQMNMPYQIYIKESKGNEKCLQLIIGFMHQFSIRLDIDLTLCQTVYQQGQLCYYFKMLSPPSCHAMYHGRYKYENNLIKSQFVKVDNIFLNNLVQSEKINEIQRLLFLNNTTILIKLDQNCEYLKQKISSFHNINVIDYPIAHIKLQNDISYFRLMNSKYLNFSLRFNILSAISQNKFNIQKHIQDLIETCEAGSKKLSEKSTQIMNAIFEQTFNTFCKITSNLSLVNDKDMDRKNESHLKYFMSVLKRTYQDFIELNDPTMRLAYTRRVSMTPSGILYFNKQPEVTSGILRQHYNIVEYFLRAHYEDENQDIVLNIPYITQSYYYNFMFPSLKILGQNYELFTWSSSSLRQGTCWFVDFNGAGKQRFEIIRSIGNFTNLKYDEIAKNAARLGQNLSTSISVELVGPINIIIKDDLLDKNKKLYTDGIGKISQDLIDQIRQKIKVNQKIKISAVQIRYEGAKGLLVLDENLEQKTIVLRRSMIKYNPAKGGYPNKIQILDFNKFRGGYLNRQIIILLLTLKVKDNVFQELQDEYLKRIEELQMKDASIYKYFSVDYCDEQIDVPNIMDALRAYINIGMDDNILCQGILAKLKQRGLLQLRKKTTILVDKAARVLGVIDEHKLLQPNEIYCLVQDENNIDQVEPENIQGEVLVTRNPCLHPGDIKKLNALSTQEILKRNNGKNPYGQLINCLIFPASGNSLPCQIAGGDLDGDLYFICWDNRLLPPELVKSMVYDNMKQNQVQYKKIDFRENNQKFHENVDKNFDMKSMFDFLYLYLNSETLGLIDNSHLAWADKSNKIANDPNCLELAELHSDAVDFVKTGKQVNLDPKLLSKIFPDYMEKEKQSTYESQTIIGKLYRQVLQMGIQQDQQMVYGLGFNQNGLPKIEYKLLYNFDKLINECKQIKQNCKITINLEEKKDQNEQQQENNDEQINEQIIQLIQKYIDDNFKRHYESSIKLIMHIFEQIVGITQIFSVKSEFEIYSGNFSSMQKGDGLYQKKKLNVEKYQKRIIAQIIQLHQEVDRKLQTLSEIERLQRISLINFILTYSPNSHPYPTFTILGEFISQLMKQITSLEVWKMLFNIHQIWYRGVGQYLFWKDLLKIYKNDQDELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MD84</accession>
    <name>A0A8S1MD84_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Golgi apparatus membrane protein TVP23 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0600063</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TVP23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MD84"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008564">
        <property type="entry name" value="TVP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019:SF7">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05832">
        <property type="entry name" value="DUF846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8078470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="251" mass="29320" checksum="E0CB64612E79CC10" modified="2022-10-12" version="1">MINQIREEHPPINLIRPPQLGGFQGPRIAESIINQQQGGMEGGIQNTRQYWNEKEQNWLRLSVNLNPLAFANYELFKSANMDISESPNPVQCILCFILKFIILILYFLTPYYQTMKLYETIVVLGAVDFWIIKNIRKLVGLRWWVEIDDFGNESWVFETTTQEKGEIQRIHSRIFWFVQLFMFLMFVGMFIINASQLQISVSICMFFPAAFTGYNLYAFNLCNKIKRSKLGESFLQMSAGRKSVKMPLKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDM0</accession>
    <name>A0A8S1MDM0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0560083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDM0"/>
    <dbReference type="OMA" id="RWYSEYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035902044" evidence="2">
        <location>
            <begin position="17"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="50"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="276"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8076391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="374" mass="43800" checksum="F5007F71D4D33AF3" modified="2022-10-12" version="1">MKVIFTLFLSLLIAQCDKNLNSKIESLLQTQLGHTLLSTAQLALQTNTPLDRIIDTLQDLEDKYQKDQKEEDQENREFQQKCDTDLSDLNTEIDAINRMKVKIQGAIDQLKASIESKKKILNDKNALRKNLKKGMNEVDVIREYQEKIYEEKRGEVGKIIKTLEEVKLVYATKLRTNFLELQTEKTKVQPEVFAEVTKQLVSFGKFKSIGKPFQNIIDFMIQMSKNGNNIGVLRNIIDLSESLIRQIDEIWQVERKAEEERVRWYAEYQKFMNTDFKIYDKEIGELEAQLQSLKDRLAENEKKMDDLNIRLESKEYTKEDRRNECQQAAYDYTQRRTSRQEDRNIVSEVVGLLNVNMRDLKETLAQKMAAGDHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDY0</accession>
    <name>A0A8S1MDY0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0550143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDY0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="95"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="328" mass="38846" checksum="6FFD273A27146719" modified="2022-10-12" version="1">MQGILIQQQQFTQQLMRIISKEFEPSEGLELELKHSIESFFIEIKQLEYEMKESIGKYCNNCAKYKQSIIDEFHDTQHDQSKHRYDELNTEFLILKACNQQIELQKQELQQKLIDQRTKYEELNRQKKEDKDNYRQQYEALKKKAQDKIHRLKDKIVVFQTQLQTYAIESEHLRIIASEGKINSPQISSAIKFKLKSILKEVHQLRNLSNTLLLENSKIILQAKEQLQFLNFKKDIQGTIIKRNNTPPEPAKIKQYYSQSQSIQNSPDRKLKTEKPNMIRKLNVTRSSISKNLLAEFDASAQITKFIKQYYNADQQLRNSTGFHTKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEP9</accession>
    <name>A0A8S1MEP9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0550165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEP9"/>
    <dbReference type="OMA" id="FIMINPI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="525"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="638"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="669"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="705"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="771" mass="91218" checksum="EDFBE62A9CFDF6B5" modified="2022-10-12" version="1">MPSLNNIKLSINQPPQCSKLFITRSSDLALNDIQVTTICQQSNDFPYRYQLKLFLRESDLIWFLQGQSDYSLIYYPFQNQNYFSFKISTSVDFSAVGVLIQVLDGGGSISNVFEKITQKSANINCTSIQYQNYTLQNKISLLFEAMNQNCDQLHRRIYHDLLYQSILPDSNDNNLKFQAIKLYNKFLNKSVSIKPQNRLLNEESLQMCYDQYTTYFYVTNPPTEIQINLTQKLEGLKENIVKLDAALIYLNQLKQQCEEELHRNIFVWNEEIFQQYENSQDGLINLLYFVDDIYSDFSTVDIKNETTYQMIVGLLKYIPMIIEITQNTILVNDKPIVIQGDQILQQIKRRTKQFFNQQFNIDATQEDYLIDFIQYESTYLLSNPLRFSADFDNLLQKELNDESLKIYSKYYYQIKLKNVYKNRFIEFEDFPSKYSTKFGNYQICTNDTQILKVYEIQCLLKTLSGKIHKCKVNRILNNDTMEQSCECYEFGDIFLTTSTSISKVIVNNTQQQELEIDNVFSLEQIFVLIICTGSLTLINFTIYIFYLIKDCQNQKDIIIEKSNLNPPFHQDQKTLMYQGNSIVFKEKFKEIHQTVSLFYYSDQDIQLSYRILEVCSQFNLLLSLTILECSNQTNQIHFIFLFIMINPIIILILRIFYKIIEAIYRFRRIAAFISNFILIMLLMAPNLILLIFFKLRIQIQQEQYMVAIIFFGNILISQILIEPITVFVRIIFYRLITTSIKNMDMNPIYHLMHFFVMHSSLEEIFEEFTRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNX0</accession>
    <name>A0A8S1MNX0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0670114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNX0"/>
    <dbReference type="OMA" id="IFTECEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="140" mass="16678" checksum="EF4F4AD499EA1DE1" modified="2022-10-12" version="1">MEQHKQYCKISKQDRKQIIHEVLVNKRLILEVAREFKVLPSTCKSIINTYIKEGRIGKKERRIRKLKKVTAIYTITLNPLNPLSSTIFTECEIAEVEQPSLQSNVQQQELPQKKPYDFLSQWTENYIQDYKKYKFGSKYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPD4</accession>
    <name>A0A8S1MPD4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0650097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPD4"/>
    <dbReference type="OMA" id="QIYNIER"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="159" mass="18762" checksum="47A3B1B1AE5C8CD0" modified="2022-10-12" version="1">MQIRTTDNQIVTINIEAFLKYSQLFEHIYECEQTTNILDMEIQSNILMKINQFLIDMLPENPSVIPKPIPNTDLDSFLNPIESNFLKSLQLYNIERQLIRKAADYLGIKMLMNLMQVSFTILFIGLTPNQFIEKYQLKINYTKELQNNIDQIYDNIIIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQU0</accession>
    <name>A0A8S1MQU0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0660132</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQU0"/>
    <dbReference type="OMA" id="NAKIHFT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="2">
        <location>
            <begin position="2"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="181"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8081712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="486" mass="57737" checksum="7765EF923FE41AD0" modified="2022-10-12" version="1">MKAFRYQVTLNISKVELSVKFPCTLQVMWKRGLNKCLTRTKEKTKNIFIVNEVLTFDFNVGENTQKKTHLIALLNVDGQSKLAGVVNFNINETMPIEGQCELNLERSPDPNAKIHFTYQVVNLGETDLSDSRTSESFHQKTLAESFGFEKENFNHHRILSQKKSSNQQISNYEVQLTKELYEKSLKDLDQQKIKNSQLQDQLNQLQNEKSNYTDQLNKLKKQIIQLQNENQYLEEQYELSKQEIIKFKESAQQVNQFQVQNEKLNNEIENYKIQNSILENKYQQTFKKVKQLEEKVQFSHFNKIERSTNTQFNEIPEYIQQNTTITQLDEQVNEYKRKNDKLTYELQFTNNKLKTIEQEISQQKSQIIQYQTQLSQLKDQCNRFETENAKLKILIKQLEQDQNNKEKVQTKSQSDNQKQIESLKDQLKQNIEKLEQVKEKYRQAKEKIEKQNEIIEQQNDELTMQNMTFLKELQLIAKRQEQFGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTD7</accession>
    <name>A0A8S1MTD7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0630049</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTD7"/>
    <dbReference type="OMA" id="CIHPEST"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071913">
        <property type="term" value="F:citrate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF4">
        <property type="entry name" value="TRICARBOXYLATE TRANSPORT PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="7">
        <location>
            <begin position="11"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="7">
        <location>
            <begin position="106"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="7">
        <location>
            <begin position="203"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8080015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="32915" checksum="E632041AC376CDC6" modified="2022-10-12" version="1">MAEMESKQNIKPAMLNMKNFQIAVGATLILQPLEVLQTSMIATGYSKHNINFKGLSLIGSQIYKQEGLRGFYRGTILSIAKNTVSFSFFFSGIEKYSPPAHYPVYLQSILNLMIASTSKLAGTIASTPLAVMRTKMQIIGNNEYRKIDKCFIKIAREEGFFGFYRGVIAAILKDVPFSGIQYTIYRNLLNFSGIFTKGQDPKTNSIVVAICGSSAAVLAILVTYPFDNLRIRQQAQNKSSNLLTLAKTIQQTEGLKGFYQGYLPRLIKKCIQSGVLWIVYEKLALKQRKKNTEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MVA6</accession>
    <name>A0A8S1MVA6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0650149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MVA6"/>
    <dbReference type="OMA" id="KTCCEIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32046">
        <property type="entry name" value="G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32046:SF11">
        <property type="entry name" value="IMMUNE-ASSOCIATED NUCLEOTIDE-BINDING PROTEIN 10-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="2">
        <location>
            <begin position="136"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="531"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8081296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="663" mass="78920" checksum="3F94E72DE5134F76" modified="2022-10-12" version="1">MGNQQTQQNLKEDISDSNQIVTANLSGIKELEAEFLNFINDKKRRDRWQSCLKQFKITSMEDLISAYGLDKESKVGRRCNEAEHLILNNYFLKKQKEYETTFEEQKLITQNYIQEYVKNNKFQNDIIRIEDKEYHIRNILIVGITGQGKTTFINSFITYLKGFNTHRSYENFHFRYYIDKPNNKDVKSHTQNVQSYQIIYEDYLFNLIDTPGLGDTEGLEKDQRIVDDIAKYLQQNLYENNQNLHAVLIISQSSTQYEVIDNKLTLLQTSMLSILKLFGKQMSNFAQHCLSFSDFTAANTLNFESPNSIFYSLTEQQLQFIHEYQKNVQEQSKQFDDPNFATQMQLNQKDNMYQQYYQFQNSVFQVKKLGFIEKIQIKKNVENYFSITQRVKNSLGFELQNSVEVIAQRKILKQERDQIHLKLKNLITNKNRINDNIKKIQIYQNRIQESSNFEFYEFVTECKRQFQPQDGMYFTNCLQCNKTCCEICPIKNEYLSNCVQMMRSNNQIICQSCNCQLDKHINEKYRWSFTTEQIKRINLSLQQENENAKSQFDKLNQILVKLQQSLNDIQEDIKITLIQMKKCFELLLSSALYTVDIQDEEAYDFVLRLYPEYKEAFQEFQTQEDMKVSQKIRQDIKEKKLLISQTLLSIKTQQFQDQSYSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWD8</accession>
    <name>A0A8S1MWD8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0710106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWD8"/>
    <dbReference type="OMA" id="DITELLW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="595" mass="70903" checksum="4414DDAB41D0EBF0" modified="2022-10-12" version="1">MKQEAMQEIEKFQKNYLFDINFNTKGLVKLLGQVSYVYDNHDLIQSPQFQEILKSSKENLYQFQNIRELALLLNFCFKHQLGDENLWFAAYQHLLNTQSQIQVEDLLLILESINETNLEYLKPLFKQFENAILEEVQRNNHKVTIKALQIYRKFQPDNNNFFYSIKKQIIDSLPQTNTDSVLNYLRYYGLGNEPIEDRQEILNAIMIHLYKMQKFLNKKQLLQVYYTYVRTQLGSDILYRLIEDRVAILIDEFQLNEIEQLLIIAANQKNNIIFKYAERLLKQRLLDCNPADLVQIAVLFIENNQGTPEFIDSLEQHLIQAIYTSDDITELLWALVQKQYNSKWMEKIADACKERVPNLSVKQLTSIVWMTSDYFKRLKNKEKQQSKYIDLYELIEKHLIQYFEEGEVINRDVALVMNAYIRNIPISQDLVDLMEIYILNSTEEELQELDGWSVSQIIWGFSQIEQYQNLKEFLNFMIKVTIECIGEMKIDELFTVLRVYINEKQETEEMIKEAIEKTNFWMDQLDLNQVYLGIHIFTNTSFSNLQGINELIKKLLDRLEFLKSIKPQIDPNEDEEIDHDKEQKLRLEVEKYKYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXJ5</accession>
    <name>A0A8S1MXJ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0730139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8085088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXJ5"/>
    <dbReference type="OMA" id="EMNTQFH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8085088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="322" mass="37433" checksum="81E9B6EC8C541D07" modified="2022-10-12" version="1">MESSIKTSGRSKRALNKTLSKNYATQIIDLLSNKNQLCLFGSITKNESPINRIRILRSLRIRTQEDQQNRLSKSFDLKVVHHTRQQQRRKSGIEQLSEKQMHLDCLYPKTCVQQQQILLNEIQKTKERKKTIFKRMSSILIKHQTTLRNIGNQNSINNQRNSEIQLLMLQQSQQPIDELALLQSQEVKKTKIPQSIIEPELQKVVLNVSRKCKKPFASIQSYVNQKTEKHQSQKELGNSNPFLSYMSCQSLTEMNSQLQSNSPQTQRKLISLKVACPLPEIKLISSARNYGNTNDINFQKQKFKNNIYQGDLQRKKRIETKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MY84</accession>
    <name>A0A8S1MY84_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0670223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MY84"/>
    <dbReference type="OMA" id="QIIICIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="841" mass="100853" checksum="4FAAAAA9718BE125" modified="2022-10-12" version="1">MEQILILIKQLYNENEEIRKLSEEELNNIFNDFEKIKIGFECLCNQNIEKSLRVQFFIYLKHYLQQNYRTILINHKDDILNILLFYLNKVDQQIIPLLCKLINFMILNTPDSTQIIICIMDKLLLDISYIQLITEMMSNQIVKIKLKLSGYKYNFSKIYQFFNINDYNIIDLILNFYISLTNSLIYLLEDNDYITIINQYCSFISNNPQQSNILGKFSKIIIRLIQKKLPPEHIQQQLFSIITYNYLDSQYHLDRITAYRLLKILNQIQTSQKINYQKQRELLIFIVINIIYLDLHSYQYLYLNFDDENENDYYLLKIRNFGIDMLLTILKQDQQNKIFQEILLISDQVVPFHTNEESICIKKEAHYYIISNLFYKIPIHQIDQLFLQELLQCLKQTNNIYIPIKIQVLILLKRFLLKQIIQNQSQIDYLLSILKEQLQIIAQINNPNLLCAMIDLLVCLQLFYPQKNCLNKQIYLDLKLYYSLQNKLIFKQSIIKCMVELEQLHLGDNSLILIVQQELLKAQQEQNFQKYQLKMLIDVMSFIVEKQYTYQITLYLNIIYTLVHSSLQQLKHQQASLNLLNLFLKKLLIQQEIKSNDHEISIRMIQILFIDCLDEDPDLFENIVQTYYLILLHDVADLNIALQFITKYVKHLKNQNFLSLFCLFLFRLKKGLFQQEIQQFYKQLYEDIEQDDGRTSNELLCGLSILDINQYEIKEIMLNQQDVLYIMTNKAIQFITYKNKFYINYLINFYQSMITKKHLIFDMEQLSLNNSFLVKLHEFITQLVIKQEDTQYSIINQIQKIFEISYLTKLFINDKIIQKFQTSIIMIQSQDDLNLNELKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MYG2</accession>
    <name>A0A8S1MYG2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0670056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MYG2"/>
    <dbReference type="OMA" id="ISDCFEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="134" mass="16145" checksum="73A72C038CE45513" modified="2022-10-12" version="1">MKKFLNFDLQRSSYANPVMKEFAQLSLLKKIESRKLLEVAELGQRCYSIEKNDNKWLEQISDCFEHYKIELQRVGNMLKQAEYEMMQIEDEFMTKEMNCYSLVSEFEMESCIKDYKLQCLNQKQKVLDRINELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZL0</accession>
    <name>A0A8S1MZL0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0750101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZL0"/>
    <dbReference type="OMA" id="NQIEQCL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="172"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="246" mass="29132" checksum="0AF947ABADF5D971" modified="2022-10-12" version="1">MPQESIEIQMLKQQVQLLQQELQQIHQYGNIRILWNNIFSKLETITFTSQQPLKIYQQLMFRQSLSQDQIKLEYFSQDAECQTDDDQIKLNVQDTIKSCIQSIKIELKIQQEKYFQDNLNQIEQCLENILNYSKHKQNKSNQKIRSSINSSLSIPTTIINLSTKENYITKPISNLEQELKDSQINELKKQNQALKQDIQKLQLIQNQLSTQLEEKEAKVKLLNFMVLNHQQQKIEFGFPQNDNFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1S6</accession>
    <name>A0A8S1N1S6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0690138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1S6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="83" mass="9721" checksum="930B29CF11E5FD5E" modified="2022-10-12" version="1">METFSIIQQIVMHGNPSILLNLLLLIIHILQIFQLKLFCTKNNLIYLTDTENSLYLFFPETNPLPMIHDLSDNVDLNLLIPYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2G1</accession>
    <name>A0A8S1N2G1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0780199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2G1"/>
    <dbReference type="OMA" id="PYCENIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="111" mass="13209" checksum="8ABA36EA31925B5B" modified="2022-10-12" version="1">MLSEPFVLLKNYRQQQDAPKTLQKKKLIPIQKKLIKKTPKSKPSIIEIDRNPQQNQIQIQQYIEPISLIQQYQDDYLNQDQYSPPPFNELQYTFHDSYSEYINLPDEALGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N597</accession>
    <name>A0A8S1N597_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0710239</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N597"/>
    <dbReference type="OMA" id="WYITLFT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000003">
        <property type="entry name" value="GTPase activating protein (Evi5)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000085">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF4">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="4">
        <location>
            <begin position="107"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8084225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="371" mass="44295" checksum="4C38767AD324AF19" modified="2022-10-12" version="1">MRSTQLTQCIQKEEDDQFIYEFDNLSEPQQNLQTPDFQATKQNEANQQVSYSAQIQQTDQYGFKSQINLIHHKDRKTLNARVEKWRQMINEFDKQNAQLIKERTRKGIPDGLRILAWPLLADIKKAKEKSNTIQKMSKYSDFLTKNEFKFEHQIRLDVHRTFPDNINFQDQTVLSESLVNVLKALSEAIPDMGYCQGLNFLTAALIMVTNDENAFWILYRLMTHYNLTAKYKDPNSLYREFYILDNLIQQYHPYTSKILKRHNIDLFYFTTEWFITLFSTALPINLFYRVFEIFLVEGEKTIFRCALAIIHFKEQKLTQLNDFENGLQYLRSNEDLFHLDSNQFINLMLTKYKFSKNIIQQLDQKYKKNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N8G5</accession>
    <name>A0A8S1N8G5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Radial spoke head protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0750039</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N8G5"/>
    <dbReference type="OMA" id="MIVRISH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001534">
        <property type="term" value="C:radial spoke"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22963">
        <property type="entry name" value="DD_CrRSP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006802">
        <property type="entry name" value="Radial_spoke"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13159:SF0">
        <property type="entry name" value="RADIAL SPOKE HEAD 6 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13159">
        <property type="entry name" value="RADIAL SPOKEHEAD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04712">
        <property type="entry name" value="Radial_spoke"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="408"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8085743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="50635" checksum="B7B9A6E640EED2A3" modified="2022-10-12" version="1">MLKEKLQEVKDQNTNLYEHLNNLLTKILLDNPKNAFELFEDYSFQVKSNGFKFEKYDEFDNAERSKIKMDQSIQQKTLKLINKINLGTDEEPQEATQTGYLPNLIEESYLYEWAGIGIDENYKIFKALTLLSVTKQASKLRFWGKIMGRQKDYYIAEGIATTQVEDGELPPEVEPRGTGVNTKSYWACNDLLNDKDWIELPLITPQQINTSRQIKYIFTGNLENDVITNPYFFGKEKHLLKAQIVRITQSTVIIPKGQYILREGEPREIDPAPEYKLPGFHDLKTLNDWIHFNQNILNCGRLVHLIPSELPEGIEPEAYQKQIENADPFENRLKPISNDKNAWKIRIVGDTTDYNPLSKDTNNKVNNGVIIIKSLVWPGLVTVYSNKVLSQLYYGYGYKATNNHFYPKQPQQVQSEQPDLPEQPEPNFPAEVPQQPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9T1</accession>
    <name>A0A8S1N9T1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0830173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9T1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="47416" checksum="88D6E422A4C60012" modified="2022-10-12" version="1">MLSLSQHSLLVLTDDRYINYFQDQILFWQYIIPSSVDIKNATMKYCQMTYLVYFISNKVYRFNPYDKNLQEFETFLNYQPQQIEICDQFIFVIDLQRITIYNDSVQVYQIDIKNYINITQLTILNNNQNGYNIFILDMQAGLLKIEWNQQSHTINQLDLIQNGVALGINNDNNFYVAYKRQYKYIIAHYQIIDSQIQIVRKFQSKKIIKKIIAQKDYVLLLTNNKIDILYQNKIYKILKFGIKDISIISNKIIGISTFDIFQYLYEPIPNYVQCFYQLNEILPQQLDYQLTFNQINITIQKKRIEFSVELQIHKSDTMKIFIGFLVCGIVLTLILLYTFITLKQKNLLINEMENNLKKSKVKQVPLFKSLECAISPKGQHSKFQSFDQTSNFDLNKLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAQ7</accession>
    <name>A0A8S1NAQ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0800196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NAQ7"/>
    <dbReference type="OMA" id="YSEPPNF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="207"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="393" mass="45799" checksum="A3A8ABE39AEF83CA" modified="2022-10-12" version="1">MSIQAVNYEVEDMGRYFKSSKKKYKWTFMLEDKQHMLELEFSYLSGKRRLVLDGRVLHESMMLTSSFQYPFTLEGFALNVIQQGDAFELRINNKVFSHLYTQQKTQNEFVYDEKLNTNNKTDNNFYSEPPNFNTQFSNNNNFQNNSQKINLNIKFENHKDLNPIQQKPSITQQTKTSTTFGQPGAFEEFEQFGQVSKTSNNPFGDSFQVQGFQQGQTTQQQQKKVDLLNLDDDIPQQKPIITQDQEPLFQQQQQQQQQTINPQQTNLFDAFGNGWNQQQQQQQPQQQQQQPQQQQNLFQQTNQISQPFDAFNSIPQNTQGFQNQQIQGGFQNSQAFGQEFPQAPQQQQNLNILNLYGNQQPVVGNLAYSAFPQVQQYNQPVPQSQPTPFDLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCS7</accession>
    <name>A0A8S1NCS7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0860030</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCS7"/>
    <dbReference type="OMA" id="WHCTGAS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="393"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="416"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="485"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="64"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8090428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="563" mass="64502" checksum="2777B5C2C0B880DF" modified="2022-10-12" version="1">MYEDINLQHQTNTYIEQKDQIEVIDNTEEIEFKQLNYDQALSQVGSSGYYQKRAFIIFGLQWMITTWILFGPVFYFIQPTINCKNDLNCYNECTTRILDEQCLSYICEDTSRIEFLVGNSLNTAFNTVLPCNKTLQSIIKSIVYFGSLTGFFIFSFIADNYGRKLALTLSWTITTIGSITLASSQNFQMITSAMFLLGFGGNPAITIHYSFINEHSQGHFRELQNVGLQVFFAIGEFTIITLAYYVTDWRSLAIIATIPIILLNFANLFILESPQFLYSKNKSQCVQTLNQIAKINSTKSIYIDELITNPKSKEKNQKMYTAWDLVRYKSIRYIFIACLMMFFGIQLIYYGISFVSDQLGMNFFTSNYIIALFELTAYLVTDFLVTKLKRKKNIILGFILVGLMSQYFLIQFDNSLFIIMQGILAGLMRFIICVIWALAFVYVSELFPSVIRSLALLLVSAGGSIGSIVQAFLNTLCQKYQVHPMVVFGIIGMLCGFLLLPLKETLNQGLIENIEEEEKIMNIKYQQSIETEESGGVDQMNNVNEEREFLIIKTKNINTQKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDX9</accession>
    <name>A0A8S1NDX9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0800107</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDX9"/>
    <dbReference type="OMA" id="MFENTNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34969">
        <property type="entry name" value="OS01G0621700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34969:SF1">
        <property type="entry name" value="TH1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TH1" evidence="4">
        <location>
            <begin position="639"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="825"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="268"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8088141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="953" mass="111975" checksum="91812D374FA714FA" modified="2022-10-12" version="1">MFSDLIDQFRQTYQNKYLTLLEFSNILDQYARLDIRCDLDDNVKEQLYKFIKEKQQNVTYCALETVILEFLLQQEVIQPQSQINVDTLGKCARRLPLDMLQNINNDFLMQFKNFYKLIANKYGPIVDYRELERLGKELQRTIKINSIEIMRYIQPFVDNQQKVKIDEILELSNDLERLINDQTRRQSKGFENRKQNTVEQQGEDYHYSSEEDNQKQINLISSASNDRRNFTNIIYQQTDIIKQKFINKGGLSYLTEINKATQCIEGLIANLINDIDIKNKKIEELIIEKELTQGQLQQFEIEIQNKDQQIQLCLQDNDIFQTQINQIQDQNKDLKIQLNFYNEELKGITRLETELKEMQIDTKNLREQLNKINKENIRLQKNNKSLMEIIEELEIKSQQNKDIDTIKQSLQQHKDQINQLQIKLEDLQIQNQQLEELNQIYEKAKEGLEQQIIELKREVTHYKKMFENTNIENQQLRSSLIPRTSMFNSQQMNGGGLGRLSKISIGGGITSSKIYIIGRNSSQRMSSIRGNQYGSTFNPLDEIQSGPLKIEEVQDIDNQNPDQQNVNSNKPSMINNRIVARINIKYENSIVEVINEENEDKEQFVTNNEKPKEHKLLQQFDKFWGELGTKNLEELAPDTQQIKIDENILYYRDFLGIRDDPKIMEYLKLEKNIYKSIIQRCFSDGVYRIDAKGKKARRILFLTEHTFYIFEGEKKPSKLSRSFPLKNIHTLVFSEASPVICCIKVAGSDDYLIETFKRSELNSFLTDIFASQKISLFKVEFLVQFKIKMKGVKDEIPISQIIKKQTSQDQGKNPTYKVSSKQGLYSQNMGWLQIFKKKMFQGDWQEVFVILTNVGLVLFKKPGDAEPILFISFVDAVVIKNPLFDTTKQHTLKIRYENCDSEFVFNATSNLLLDQWHDAIQNAIMEQVRQQTQYMVHQQQQVVEHRNQTMVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NG92</accession>
    <name>A0A8S1NG92_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0830005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NG92"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="44" mass="5125" checksum="B5729ACE6E4A74D2" modified="2022-10-12" version="1">MGSSINIKLDQQLLFFNVIIFSMWNYSSQVVICQISLVFQLIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NH13</accession>
    <name>A0A8S1NH13_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0890176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8092018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NH13"/>
    <dbReference type="OMA" id="WMLAYNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="2">
        <location>
            <begin position="248"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8092018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="434" mass="51220" checksum="75747C4E75BA4C8F" modified="2022-10-12" version="1">MSDQEYDYDYDYDENQIDQEEDQDEIELENNYNRAEDEWKLNPDMAFQLFNEVIQKEKSKDINSRKWSFKSYQFLIQIAIMKSKFQDDQFLEYIQGFLELLDKQYKTEGDKALKIVVDALMNSNNSHLISIVLPNLLDKLKFMNQIGIYCGASMKLCKDYFQKGDYIKLEETINNIQSVLDNSQIQDEDRKKAFISELLAYRVLLYNITNRQDQIKPIYRQLLKSNLDLLEPYISGVINLTIGQQKALERQYEQSRKKLMEAFYNFLDASSPEAKQTLIYASVISILENFKMNLFDDMKSKVYDQDPMVKVFQDLRLSYEQQNVQTFSNIINSELFQKDPFLAQLKPYLQQIIVECKILRYAKCYSTIKLDYLSKQLLIEIPVLKKYIQGLITKKQFNGLIDEIAGYLEIRGEVAETQTKALKVIVDQIKIFDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NH19</accession>
    <name>A0A8S1NH19_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0800184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NH19"/>
    <dbReference type="OMA" id="TCICTHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="264"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="390" mass="46578" checksum="AC752FB426FB73B1" modified="2022-10-12" version="1">MSKICQKHKKYEEKFICIHKTILQKEGNKFSCIKCLNWKKSDDYLKSMEEVEEEAKQNVAKYVEMQSKIINSNNEMKQKYLQSLTELYHKLTQYFENQQKSITSLVEQQNQILTQNINQANSLLNEQSVLDSQSIDNFIIFTTLNEANQHLIENKYVSINKYLERQFNYEFAEIQCKVNQLINMEFKFGNIPKYEVEQFKILTADKKKVQFPYCQDHQMQKTCICTHEDCCKQQEQDYFCISCAMSNHDSHLKGNFIKTVKKIKTEQNEKLKIIKEQYEILKQEVENRIDKELAANQQQQQENFVNSQTIRENLKRLEAQIKINPFDQNQNYIFNNINQNLLSYDQDMIKLEFQSKLDQLEQLKQHISTIKEEECQQQCQTTEEQSLTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHL6</accession>
    <name>A0A8S1NHL6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0810088</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NHL6"/>
    <dbReference type="OMA" id="TKFIDKG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04318">
        <property type="entry name" value="EcAsnRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000016">
        <property type="entry name" value="Asparagine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00534">
        <property type="entry name" value="Asn_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003037">
        <property type="entry name" value="PRK03932.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF34">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="8">
        <location>
            <begin position="234"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8088485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="567" mass="64713" checksum="844E704B2C054E77" modified="2022-10-12" version="1">MQYQFLESDELFTRIKTITDFAKQTAAATQKQAKKTEKLIGEIEKPINVPPLPAGFKVDIPQFDVTKRLNKLPRSWDLPQQLYKKHLPKYNVGTIYRIQELLNVGEGYIGQTVTVAGWARTVRDQKNLCFIELNDGTSFGGLQIVVEDKLSNFDQIIKANTGFSLKVTGNIVKSPAKGQLIEMLVADPTKHEVVIVGQADPQEYPMAKSVQKPETLRQKAHLRPRGNFFSAVTRIRNNLAYATHVFFQNNGCLYIHTPIITGSDCEGAGEMFRISTIFDNDIKKIPQINGKVDTAQDFFKKEVNLTVSGQLQVEHFCISMSNVYTFGPTFRAEKAHTHRHLAEFWMIEPEFAFADLFDNMEAAEGYVKFCINYILQNNMDDLKFLDNRVKPGLIDYLKDIVSKDFIRCSYTQGIDILLKAQKAGAKFENSDIKWGMDLNSEHERFIAEKVFQRPVFLYDYPKEIKAFYMKVTDDGKCVRAMDMLIPQVGELIGGSQREERYDVLAQRIKECGLKIEDYGPYMDLRKYGTVPHCGFGLGFERLVMMVTGVENIRDVIPFPRYHGSAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NIH5</accession>
    <name>A0A8S1NIH5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0910023</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CIHLSFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="497"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="1145"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="1200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8092578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1273" mass="148185" checksum="BD2536CD32593630" modified="2022-10-12" version="1">MKGKQILIQFELKEEFYYHSYMNFQKKLRKFLEKLWLNSVFIANFIQGIWWVSNTLWLTQPINYFVFILQLACLLVRCFQQFLITKEYGLKLVIHIVSLLYYVTYFESFLLIESHQHLQVLTNCLLLIFTYMDYLKIAVLKYGLICKLGIPLYLIIRMIYKLINDFSLYELESLIIISLLFIKYTIMLQITFNKIIEEKTNKYQLTIKEERDSTPILQNQQIFPQIKSQSKVSQSPQHNLSVFIRDPPSDMRLIQYSNLSNFQKINKSFEKSDHSMSSFYNNLLNLFPQGILILNSQLSVSYMNNKCEKLLDCQGSEKVLEKVKACVDNAKIRDQQMDEQSSFQAKYQSKQFHYHLLKEIICLLQKKNIPIDVFDIMIYPQKYQYLFETNSNPIFADAQLNFMSQVFIYEWFIKSGQNQYSSQKKLKLIIIPTSMTNQQQEYFQSSSQIQSCSRVNFQPTNEIENPILLIIIKNITQKHKFQQLRDEQIIHHSLIKSFSHELRTPLNSCQHMLNLLKQQQSKEEFQQCLAIAQNSNALLIHQINDIIDYAAIQSYQFSYHISQFPINQIIQEIEKLYKDLMLLKRIQFKILISKNLVSFIMCNDKQRILQILVNLLNNSIKFTKEGGCIHLSFIEKELFSINIEVKDDGIGIAEEQLHLIQSSLYSTIEFGAILKSHFEIKKKGLGLSIVAKLAQGLTESQDNQLIIKSIKNQGTQVSFQVDNLQFNQNIYQQSSNLFTQQTGKFNQSERQYTFNKIKLDEKGVKQMNNSFSKVQDDEEIKDQNSETYKATSRLNDFEIQQEILLPISVEYFPHKFISSCKNLDPSKSSYQKLICQNCIHVLVVDDIPFNQIALKMMLKHYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNIINIKGGCIHLSFIEKELFSINIEVKDDGIGIAEEQLHLIQSSLYSTIEFGAILKSHFEIKKKGLGLSIVAKLAQGLTESQDNQLIIKSIKNQGTQVSFQVDNLQFNQNIYQQSSNLFTQQTGKFNQSERQYAFNIIKLDEKGVKQMNNSFSKVQDDEEIKDQNSETYKATSRLNDFEIQQEILLPISVEYFPHKFISSCKNLDPSKSSYQKLICQNCIHVLVVDDIPFNQIALKMMLKHYNIEADSVFDGFQAIEKVKQKMQQHCQIYQLIFMDIEMPGMDGFQTSKQILKLTQKQSTIVICSAYDTQENYIKGSKLGIDTFLPKPVNQEELEVILSKLFNIKEFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQU7</accession>
    <name>A0A8S1NQU7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0960077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQU7"/>
    <dbReference type="OMA" id="SYALFHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="190" mass="22627" checksum="369524440CEC85BB" modified="2022-10-12" version="1">MAQDQRSPFELRIWHFYTEEEKSITLLNAYKQRVLFSEDIKSFLLKDRLNTWSYWGFPLACYALFHYTGALQPYIATKYSVGAQRMIPAAISGLLWLGWVHFNPFYTTLQSEKLSLLNLIERRVGLNMKALNEQVPRTWTTQEIHRQMREAYNNRHGFFTNILYPSEERASPLQDISSYPFKYRRDKIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWF3</accession>
    <name>A0A8S1NWF3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0940105</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWF3"/>
    <dbReference type="OMA" id="LWRSHPD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="110"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8093883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="287" mass="33853" checksum="DC84338DD8FE6C72" modified="2022-10-12" version="1">MNQSKYINENLTSKQKFIKDFLIGSLSYFTTILICTPLQRYQMLIIQQQRNPFKSHHRTLIQILYERTEGINHLWRSHPDDLRYILSHGLLFAINDTYKKLINSYFQDEKHSKNAFIAGAFAGITHLFLNYPFLVARLKLKDPEYKKISLIENNFISQFIRIIKTEGILTLYRGFPIALLSTAIYRSIYFGGYNHFQKYLQVIPFFNNIITKIALSFIITTIAEIVSYPFEQLTPKKSRTYYNDMTEIIGKYRTMRGCLAIVFYGELFSIYDKVMTSFILDRDELNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NX46</accession>
    <name>A0A8S1NX46_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0970084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NX46"/>
    <dbReference type="OMA" id="THSYGDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="585" mass="70037" checksum="192034E31EB786F3" modified="2022-10-12" version="1">MLQFISKRPFCVKLKDINTLLSRLSTNIKELNYVNRNIDKAIKSRDYQEILERLPNSNSQLDNYGLQCGLDSFSHIFDHLGYDRKLEPVRQFLSKKADNLLQYPINVLCDTYYNLTLLRSDTNKFQDQILTHIKINQSIPGEQYVRLLRALTRKFNLTEDDHQLIKVLNQLNIKQSQVQILLKCFRYFAELHTLHQKPLYAFPDLFEYIATQIKKFPTHSYGDIFCAYHHLGTTFDQTFLHNTVKQFEQSIQTYKNGMTQLAMQQYITILRIANKLDKSDIIKFNWDLHFNVLKELIQQRDFQQKNTITSFCSLFIDKISHFNQNEKDKLFEMIISFNYQTPGIWDFIYQNREYLTQQQNQQILDEFIKYGQEQQQVSDQKYPQNYVFYNKYARLYNLPERKEQQFLLGGLVLSQYEQTTDEVQIIKFLQNLIIKNQFWSSQFKILLTSINGIQTLNFWKQQTIKNTGRVKDEYQIEIQRHLNGDYYLFPKVFNQFQCQFIIWSFQHFQIVPVLVAPIFYRSLGTNQNLIDELVDLFKLHKCSFNEVGYIWEQMNYPNNMKILSFLKTATSEDSSQRIIYKLKEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NX72</accession>
    <name>A0A8S1NX72_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0970104</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="SIWISKW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000080">
        <property type="entry name" value="ABC transporter C family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000063">
        <property type="entry name" value="Multidrug resistance protein ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000610">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002673">
        <property type="entry name" value="Multispecific organic anion transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="138"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="318"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="752"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="792"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="889"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="978"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="103"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="443"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="756"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1070"/>
            <end position="1303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8094931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1311" mass="149918" checksum="40B3371B0FA1C5FB" modified="2022-10-12" version="1">MDLPSQFNLELQPVSNQQLPLLSNSEEESHGEKRSLLTLLFFLDVYPMMKQSQKVSLESDQIKELRLQKRSLKMHEQFSSYLQKDGKLIKQLLLFFMSPLLKVILILLVLTLGQLCMPLLIKTVIDFIKSENRDENDAIYLILAILFLRIMNIFSQAHSRRMMLCVGYDAMSVVSVEIMRKCLRVSLISTTEWSSGEITNLIQVDAQKLILITSYISSVLMIPLQLGISLYLMYSMIGLSFLIGCTIILIMILFNIFTGKQIVKSQRKLLKDKDERTKIANEIFSQIKFIKINALEEHFLSKVEEAREKEIKSIKNRLYYSAINIFSVWLTPQLILSMTFGLYVYLGHQLNPSTTFAIISLFQILQQPLLQLPIAINSLIEAKLSLKRISKFLATKDLMTNCIHTSEFRDPTAAVDFQNAVFYWNKQINNSVELKANLEDQNEGIDNVKESFQAQQSEQPILKNINLRIEPGKFVSIIGDVGAGKTSFFQALLGEMIYLEGYGQPKIRLNGKLAYVSQKPWIQNASVKDNILFGKQFNQQQYDNAIYYSCLTQDLQILINGDQTMIGEKGINLSGGQKARISLARAIYSDSDIILLDDPLSAVDAHVGNFIMKECLLGKLKQTTRILITHALNYCKYTDYIYLFEKGEVIEQGTYRNMLKSQKFQEIKTKFNNNYNEDLEDSILISNPLDDLKKHSKSDSNSNLNSSTTITLPIKSTQQDEVDDLMILEERQKGNINYDVFLQYFAHNGGCLSFSLVMVIMIVWIFCYLGSSIWISKWAALSSKDEEFSRNTLYFSIYFTFGFMQAFFAFLRGVTIIHQSIKSAQIVHNKMMKTLIYAPQCSFFERVPQGRIMNRLTKDINSLDTEIYWNISWLYTKVSQLISNTFLNVYASTYLIIFPIMGFFLICFKMNRLYMKASRELQRLELISKSPILSYFTETLSGLTTIRAYQQTNEFLYNFSKKIDTNKKIYYKQVESNAWFLQILGLSSLIVNISAIVYCIYYTQNPAFAGLLMTYASNIDVNILQTVESLSLLENGIISFERCLAYTNVKSEKRNEDNVRVQNWPSLGEIQFVNFSVQYRQNLPPALNNLNFKINSKEKIGVVGRTGAGKSSITLSLLRILESLEGQLLIDDVDISTLSLKQLRESITIILQDAVIFNATIKENLDPLGQRSNEEILSTINQCCLNRLISNRDGLLTKISEGGDNLSAGEKQLICIARAILKKTKIVIIDEATANIDVDTEHKIQQVIQSAFENCTVLTIAHRINTILHCDKIIVIDKGQLKEYGFTQELLNDKKSTFYSIYQEALQNEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXM7</accession>
    <name>A0A8S1NXM7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0970190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8095091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NXM7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="25701" checksum="52DF83D92D99A175" modified="2022-10-12" version="1">MSLQSRKSIKHEKFLQTILEQSNQFISIFLVIQFFSLSSLYNSNSIQIKQPPLQLIIPQINQHRFKNLLILLYYFVLLGKFNELQFLILLKLSLFFLNILIIEGEALSGLIDLYCVLGQTRRNKNCFFQNYMPYTSYLINALFSKSTFFFFLIFFTLITFKERVALEYFKSHLREYFCIILKSNISTNTFNQLNQLLWMAQKLIQQVSLLYTDNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXS8</accession>
    <name>A0A8S1NXS8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0980033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NXS8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8095161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="56" mass="6869" checksum="06B122FFB076B8CC" modified="2022-10-12" version="1">MIIFIEDRNQNSQFQIDSPIMYCYGIFEVRNLQFDNVIFFLSIFKKKIIKLLYEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXU4</accession>
    <name>A0A8S1NXU4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0980043</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NXU4"/>
    <dbReference type="OMA" id="KPQITIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012389">
        <property type="entry name" value="Cyclin_P/U"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF108">
        <property type="entry name" value="PROTEIN CNPPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027110">
        <property type="entry name" value="PREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR027110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="166" mass="19790" checksum="C750CA15C219071D" modified="2022-10-12" version="1">MSVVMERGLEESLEDNIIYAIAKVLDEIVQETDIIESPIQTVFHTNKKPQITIYKYIERIKIYTYCSNECFILALIYIDRVQEKNSDVVINSYCVHRFMLACILLSIKYNDDDYYKNDYYSRVGGITLQELNSLEQELLTLLDYQLFVSQNQYYYYKEKLMKYAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZ16</accession>
    <name>A0A8S1NZ16_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0970160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8095043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZ16"/>
    <dbReference type="OMA" id="YNQREIP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8095043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="287" mass="33713" checksum="BB02949729F560EA" modified="2022-10-12" version="1">MINQKINDAKITKFPYSGHKTQSDTNIIWCHSIDQSPKTQSNKDIDTDKLSYEKMFSSNEVDLEFEDFDTKPTNSYQVVSPGLSITSYDLDQTQKSSDIQLYGSVSYNQREIPQIVLAFRKRRPAQVQISQASQFNQESWKTLSNFSSPDSSPFQNKKIQIKSFALIPLKDEEEDEIFSVFNDPYNFYGTISQTKLENMNHLQQFLTLYKMRTGTFPRQRENLENEIIISIAMNLAKIDKQLFKQYFPSKRVYFDDENDYTRTKVIKRKEQRQLTQVVTPNFQYIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P123</accession>
    <name>A0A8S1P123_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1000150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P123"/>
    <dbReference type="OMA" id="YHERPHN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="106" mass="12249" checksum="E339A3BEC29148F4" modified="2022-10-12" version="1">MSNQKTPKSHNSSIIYTPIPLRLQYSASRSSKQGSNSNILWDSTSKESFLLAHSQSPQKVLLQTFESLYHERPHNPMTRDEKFKELENLISSFDSEAFIQEVELFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P182</accession>
    <name>A0A8S1P182_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0950032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P182"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="452" mass="51004" checksum="B1D57CF669B495F5" modified="2022-10-12" version="1">MDSGSFNGYHIIDAAPYKGSRTIYLANQETLRVLIADSENNEETSYSISLSTTQSSTCDHIAATEDGVYIFATCQLNNNKVFHVAKCSTTGLLECHQFGTIQTIDALFTVSQLAVNGNYVFVLNGNPQRPRNYEGSLNVYRWTASAQQVTFDLQKIFDKAFFGNQSSYFSSFYLANYKVGVTNYLKVLLVDSKQNLYAIDASLSTFGVITWYTTYSQLNLYTFINQGQFVSQTSSYYQVLELAAPYYQSSDQTIRLRVLITTDSSAHYAISLTFDGKSISTSIPLSYQNVIYLLNQYSTWTALNKAQVGNDYAVIAYTDNSQLLLASYLLPKDPIQGVPLIKLVGGTQSKYGYPQVSDFLAYIPKNIGFPRLYANIIRNVDIKENLLQTYYLNPTYQLYVYNVGGDLAEQYVSLTLNNAFTSDTGYVLLRQFKTEQQEEDKSLLETVYDIFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5Z7</accession>
    <name>A0A8S1P5Z7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1070038</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5Z7"/>
    <dbReference type="OMA" id="ALCCFGR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052983">
        <property type="entry name" value="MFS_Riboflavin_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43385">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER RIBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43385:SF1">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER RIBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="477"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8098432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="529" mass="58547" checksum="B8B882DDD44EC438" modified="2022-10-12" version="1">MEQRDAQTNVQTNGAVLATEHGGSEQNKLLPKMSESSSPMKTDLQINQNKEIYTDEEMKNFTKRGIISLIGGVILHLELGTFYVWGSISPYVCAWMREKDKGVTLNFMAIIFPILGVITMSVLSFGIKIAERIGFKVTIGIGSFTIALAFLIISFIQEIGGFIAVYCIMVGISGGLLYMLPIICGWRYFPNRRGLVSGMTIGGYGFGSFIFNFVCKAIANPNNLKPTVVEVEDGKDVKYFDSEVGDKVPLMLQVLAASYLGLGIIATIMIRYPSEIDPDKMLATLDAEELKKRKEGAPAPAPPVLPAHKECVEIGRGMKHPSFIILQLIVLMSCTFGMLISNCYKYYGLELGIDDATLTATGSVAGVMNGSSRFFWATLTDKTSYKFTFTIISILNLITTAILPYNKDGIGYLLLIAVVYLAEGGLLATYPVICAKIYGKKIGGLMYGFMFFFVGVSNMIGYLLYAFARKKIKWEGVFWICFALNVIGIVLGLILKEVGYDWRDQKVIDAEKEKERELENKRQLAINQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P985</accession>
    <name>A0A8S1P985_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">transketolase</fullName>
            <ecNumber evidence="5">2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1090069</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the transketolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P985"/>
    <dbReference type="OMA" id="VYCLCGD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07033">
        <property type="entry name" value="TPP_PYR_DXS_TK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000129">
        <property type="entry name" value="Transketolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051424">
        <property type="entry name" value="Transketolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004559">
        <property type="entry name" value="PRK05899.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43195:SF1">
        <property type="entry name" value="FI06132P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43195">
        <property type="entry name" value="TRANSKETOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="11">
        <location>
            <begin position="335"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8099283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="644" mass="71016" checksum="DDFBFFFAFDF2DC8E" modified="2022-10-12" version="1">MSDRVTRKGKSQPQKEEPKQAKKQKVQKETYDVAQLQDIAHQLRIHSIEMTIASNSGHPTSCASMAEIMAVLFFTKAGMHFNPKDPGNFGNDRFVLSKGHAAPILYAAWSMVGYIDSKELLNLRKIDSILEGHPVPKLPFVDVATGSLGQGLSVAGGMAYSSKFLDKINNRYWVLMGDGEIAEGSVWEAAHLASHYKLDNITAIVDVNRLGQSEETSIGHDVNVYRKRWEAFGWKTFVIDGHNLNQLTDAFDQCRNVKNQPQVIIAKTFKGKYLEMENKEDWHGKPVPQAQVDFVKKQMKQQDGFTLTPVVPTQIEKPQQSHFTLEVNYAVDGKQSTREAYGKALVGLSKTDANQQIVAVDGDTKNSTFSIKYKEAVPTNFVECFIAEQNMVGWAQGFSCRGKVAFASTFAAFFARGFDQIRMGGISGSQVKYIGSHAGVSIGEDGPSQMGLEDIAMFRTIPNCVVLYPSDGVSAERAIELVANHKSPCYVRMSRPSLPILYSNNEVFEIGKSKILKQNDDDKILLIGGGVTTHEIFKAAKQLSEQDKINVTVLDLFSVKPIDHDGILNAANKTSLKTILVVEDHYSEGGLFEAVSSCLSLNNEIKIHSIHVDKVAKSGTPAQMLSLYHLDAAGIIVKVKSIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PB43</accession>
    <name>A0A8S1PB43_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1120095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PB43"/>
    <dbReference type="OMA" id="HIMLIDR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="683" mass="79861" checksum="CE20747E6F4A4030" modified="2022-10-12" version="1">MNHKSTYQSSFGGFTSIATIFLVFLFFSTNFVDYLDGKNIITKQTQVYDDDLTNLQLDDQDFIMALGIEQSNFLSQPYFTLSLQQREYERLPNGTVLKNITQLPLVPCTLERFQQIFERYGKNFSEDFERLQLKNLLCPQTQISMNIGGTYTSNNFNFLKIEVTPCKQSSSNDRTCASDDEIKQQLELNGNFKVQVYVVNKVINPNKIGSDYISIFLDDQSYLSFVPKKINKYANIYFRQYQFQNNVALYPFNEDQNLNFISIDNTETKEITDLGRDSDTIYAAFYFRKSPITILVERKNQNVTDLLSKLGGLLQISLIVMGFIISAYNKITIVFVELSNKIYEFSPDIEEQNKQHQLNLKLIDNAYEEKQFRQEYYVNREGQQQQIPNSEIKHKSKILRLFGKSQPDKHIMLIDRPTTENNECVNQVLNENEDKSNQITQLAIKDHNILAQRLNCVSGLDYFKKQINLILNRSQPLRFSMKIFLNQLCLRKAFSNSLSVLFYNQAVDKINEQLDVFNILTKLNEIDKLKEILLTNSQQLLFDFASKPIISMIEEKEIPISRTLLENRARNTNVSFQRKRTRKSQVIQSNNKSFFRTQSIDRDNRIYNRIYRAYDMILQQSADQNPQHEVNRKLIKKLGDEMQAIFKLSKLLDFDGIRRLRANSNIDLICRNTQNIPEEIMSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBC6</accession>
    <name>A0A8S1PBC6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1120145</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the inner dynein arm light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PBC6"/>
    <dbReference type="OMA" id="ISICPIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183:SF0">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT INTERMEDIATE POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183">
        <property type="entry name" value="AXONEMAL INNER ARM DYNEIN LIGHT CHAIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8100497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="15179" checksum="FFCDDF8E26508119" modified="2022-10-12" version="1">MNKQTKLIQIKKSLVKYENPIEVSNLNDAKGLQGKKKAQLFPQNQNQIQYVSHVAATREDVGNLQKLLDGRLLARQAKEISICPIREELLSQCFDEIIRQVIIDCPERGLLLMRVRDELKITIAAYQTLYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBM8</accession>
    <name>A0A8S1PBM8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1120003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2519"/>
            <end position="2541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2627"/>
            <end position="2655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2667"/>
            <end position="2689"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2714"/>
            <end position="2736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2822"/>
            <end position="2842"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2848"/>
            <end position="2868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2957" mass="345710" checksum="17FB8C7973FE5153" modified="2022-10-12" version="1">MLKDILIQKSLFSLVTIIALASCYQFNEELLIPSSYPLESTIRYDIGDFISFEFWSFYITNWIPQSYPQVEQKINFDKPKQQLLFLMKSLDDNQVLAFMYTNVITNTYVVHSLFVEDKLSQIAYDPKDYEGVWIITIITLEYDSLIYETVGSLNINYKLLYLNQAKPNSVEFLVGGTGVIKQQYELGIFRGRLSRIFIQEYYRYPWDIYDGTIYWLRLNEYEKKINILPGLAYFDGVIQKEFEFDQFGRKFCLFGWAKYYSQEAYQLTQYLLVRLTIFKNYKDEINIGDEIVKIIVNLDLSNQNNCGYDVISHHYFMPYPISIEKLATNDKITLRQDLPYFQLLTKWHFISFEYGLEEQRRKSQFRLSYYENQQLITNSYLMGDLVYSNLFINTKYYAIFGGDYTIYKKLKGQLADFKFFSNYQYDLNLNYYCHDNCQTCYGPLNNNCLSCYDDRGRVFLEEYHQCVCPIDQIEINQICKSYSDLFPSLIYDEISLDVSNSDCPFGYFYLPTNQQCIQCPQWNSYNLYCIDCLLNSKNWYIKPICTKDFITQQVSQTDDAYTQQDRSVLDYDLYNIDDSYNLNLIEGVYDYCDITEIQFENCFQLDVNHLSSTAFGFCKENRYYYNFKCKLLDAACKKINQNTQKCTECLSGYYLSDESSSCILCPNSCPNCLSLTECLSCQTGFGLINNQCQKCGNFCKVCQYSNELEIMVCLSCLDNYFYYLSLNSQDCRINELTNCLIAFESSESDPHINSLDYMFRPYSGLTFTKCAKCIDGYFFDELYLVCLEGKQKNCNQFYLSKQGVFNQQVCLYGPYDSMVMIYSFVDECPRMKLNCQYCVVEQLTSYIVQNERIPKKVSYSCLVCQNGYYAQKKTGQCLSCPQDLHCLSCFQQNKISKDNWKNDIRAFYRASIDTEFFQHKFTEYGISQNDIDYEILCTICQEGYELHENQCIEICPESCLKCILQNNKNICIKCPQGLKIRNRTLINNQCISCPNNCNLCYERDKQDVHSINPLFNNEEFSNYSYYCISGSNGYFDQELGIDADCEFGPCIKSIVLNLNLICNNEEYYQQLNSLETEEQKKSFQHSNILSDQLFSSSSFSQFETQEFYLLANQKSIKQILIKITSQQHQTCSVLDKQQISQNFSSNIFTAIQVELEIFGNDFTTFQFQQQLLIVNFKRVHIEGIKIDIDFFTYGPSILKFKSAFEQTIQLINILFWQDDPRITFQTIMQNAKNVYISNFIVQDCSRQFYIEYFIKIEKIQSKQTVKIENFNVIYSQFWDLHFFWFDLKKDDLVELNYIYIETSLFNMGFINQTNGELRIFQMKISYSDFYSNNGLFLIKNLQYFNFDSLEFISNTVHYGKIIAINNYSDLMNLNFSLNQFKDGAQLIVNDKVEIFYIYIGNLGFYLNKYHENARFIKFTQNIIPNRELIINQLSIVANKFIQQPLQTVFDILEMSLIYLDVISVKIDHLIIQKAYGIQDIVIINSEKLILKQIEIQQHENFKFKGIHELFECLEQSIINEYYYLSISIYDVPIIEITNLIIQKAESINYPIIDIKTSILKTKTKNIIQLSELHFKENLILITNKIKQASLFQISSAEDYQIEIKNSNFEKNIMHQYIQIDLSNSGLLFNFDCQHCTILLIDLNFTQNIAINTFNSIFYIQAKYLSIQNSIFVQNSQFDYLILQPYLYWGFQEDQEIFFEQIQEKFPIKVLTGNGKIVCQEIFIRNVSISNSTGSGLYIILEKEASLKIYDTNISSLSSIYQDDNEDGGAFLIDTSISSSTSIYLQNVNAQDIFCRNRGGLIFIQSGLGKTMINLQDITIKDVYSLKGSIIFCEFSASTKQSQQFVLNHLDMTNSIQGYLRFLNRFSITNSQSLIQLQFERSLFELAYAKLISIKNIILFSLNYESLAIISQTSNLEISNLRISNSTFLNSFLTVNSMKNQSKLRLVDFQVINAEIQHQIPILEKCIQNQAFDQIKQICSYDNSLKESPIDLKYFKNQDSVRNAYCIIYQMNLQQKKQISSLLDIFPQNSQIHISKLQLRNINCTICQKGLVNVNIYDNLSMFVMNSVNFYDNYCGKQSCLNIVKSFSTYRILQSLIYIINEKQYDVKLQKFQCIRNFANKGTCLQIQDIKILIEDSILQNNTAEQQGGSIYVIGHKSFFILNSVIQYNKAIFGGGLFLKDQISQNVIQQETIIHENIAAQFGDNFVQPPCQLSVSIDLKNQLPKIKIIEKDNLLVEQIQIGKYQLFVNNYSNILYVPNGQTMSQYQYFDWRNQVYIGYNLHLRLIPLDAYNNVQENLNNTECLISGRLLIENEDQNLTENFTENFTNFNSVLFSKSDYNFDNITFFLDDQLNMTLQIQFHCNSIFTPIYGKAQEILGYHNNYFLRMNIKSLPCQLGEIKSLFDYTCVLCNATQGLYSLVLNSNTCSQKDDVTTSEIKSAQLKLKHGFWRPYFDTDEISQCINLLYNCNGGWSEGDTSCFQGHIGALCEECDIYNIRGFGHFSTSTKYSCGSCVEQSKNIFVITGISLWTLISILITVRSNISLNEQMMLKRVISRLISSRVQYHSNFGILIKMITNHLQILSVIFTFKFSSLISIGNVINAISNPIQTMTHSLDCFLKDIFDFEIHYLIILWQIIMPFIYICIFLCIYSFALIILKLKFNLNVITTTLIYMYIYLQPYLVGALISLISYRQISRFLWIQANVSYRYDTQIHYQWLLKFCIPILILIALTIPLYFFISLYVNQNTLNKKSTRQKWGYLYNEYVLEAYYWEVIKIVFKELLIIFVSYYEENVVKKGILLLSLVYIYQELSQKYRPYKSFILNKLDAYSANVCGISIAVGIGIYIDQQSQTYEIQIPCLIILIMLNFQYIMKIFKEIVTSFVEENALLIDNIKDTILIKIPQIQKYKIFKKILQNRGVQKQRTQMRYLKLKKYLLAKAKYMIKCQNNYGVKISSSTKSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCL1</accession>
    <name>A0A8S1PCL1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1130092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCL1"/>
    <dbReference type="OMA" id="LRITFQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035746393" evidence="1">
        <location>
            <begin position="24"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="659" mass="76003" checksum="A8903204B95AAEDB" modified="2022-10-12" version="1">MLRVVCFFMFCQIGITSMQLAYSAFTENTFSSSQGWSVIGGSLGYEVRDGQTIFGGNNLFGAGVSIYKLILISSPHHTVKLKLKIWAINTWENESIQTKINGEIIDQQTVSSNLANSFGQFLYQEIIFSHIQRSLILEITSTLQTTSSKKYWGVRDVYIYTYSCPVGCYFCTYLDMDSSQCKPYILKMSYFTEFSFYSNEEISTFGKYSTGTTLTRQLFLEPHYQLRITFQFWKFDALQTSQYQLQIDNSIVWLDSQNQYQKISICGSTTMPEQYYDVDITINHNNQYTTFQFMSDLISPTDSFGIREMNVYILACDSMCDLCEAGNGQSCISEIKLTQIEIENDLTQNSFASQSDWGSYFPSDATGQIQKTFLTDTYFPNMNYLSLYNLLYKDFVLEDHEKIKVSFQVYSSGPKQNINLKIDEDYDEIVEIGQDIPTSICTSSLQYDIEDYPYFEYTFDHTANFVRIQFESIPYSSGIYKYGFNQFKIYSIKEDCSTTPCTQVEKLVNSNFFTTQFSDPKNWQLLSYSPIAISSCDSKSVVGGINIGKYNIFLQKRISLSQSHQMLRLKLTIYFLEAWVKTKSLRILADKVIIWDQSVDYQTNLQNLCSSNNSYKDQLVTVDISFPHNKSTVTFQFVIQNGDGSEFYAIRDYHLFYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCM1</accession>
    <name>A0A8S1PCM1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1140085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8101052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCM1"/>
    <dbReference type="OMA" id="LRICTEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="177" mass="20934" checksum="FFB5DE51D2C05A76" modified="2022-10-12" version="1">MIDQAIQFYYFMEQPISFLEILNQPLLDSQSKLNYFKMKYAHQSNKFNQLRISTEQNIISDSLMINFNIQSPNFKVQQKFDYNHNKLTIHSQIITQHATPKSSRSNSPLKRVYLSSSLVARRQFNGYVKNNKSPDPYDINHYPYDIHQQMTKKVKKKKIDTSDLISKTTYHLSKYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGK3</accession>
    <name>A0A8S1PGK3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1170181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGK3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="239" mass="28020" checksum="EB1C5AD77F35A2FA" modified="2022-10-12" version="1">MGISSITNTNVPKNQSYFQIKLEQKHNRISNPSQEPHTNINQFDHKKETIRKINQLQQYCKSQTALEIAAQKYSIKKSFKGSSDLSTVPTLIDKLNRDIINKKSSVIKDVMKEELKKKIPKKQKEINYELDSMFKNYKSFSQLCLNENSTIQKEKIYNGYLKVYNSGKNDTIFLDKIEERKKSLSDESEEYRAFLGEIDEATIQRDVYNNNIRNPFLDDKITVIQMKKDGYLKRRITQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PL63</accession>
    <name>A0A8S1PL63_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1210152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PL63"/>
    <dbReference type="OMA" id="SKSPWNR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="241" mass="28604" checksum="49AE2E516EF03F98" modified="2022-10-12" version="1">MSALILSYPKQKAMYNPQKPAILLEGNNQKCIKEVKQSHQLNVVNRLYQQDEQKKNKIKLLQKEKDISLTPKQKSYVNKQSQKLLNNLQLKPFLQRQDDVLQEKKMKEEFQKLLLIAQEEDQLQKNTIIPTRSHSQFIKDSQNWVEYKEQKKLNLLEEINTQNIYSFKPVLNKKSLQIADQKMKNSSLNQVDKVDRLSILIRESLENQKENHSYSPCINTKSRMIVEKNKQQRSKSPWNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PNG2</accession>
    <name>A0A8S1PNG2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1230070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PNG2"/>
    <dbReference type="OMA" id="YMEPIAT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="633" mass="73836" checksum="12E0D889905BD7AC" modified="2022-10-12" version="1">MKHNKEQQISKENLKIIRQSELIQNTVVWTNKNLFLHYCQSQNYYYTRDINEILADASSKAVIRYKDWHGYDDDDEYLKRYYYTDEYPQKVQLLTEYYKFHTDIARLYMEPIATMLNKYYDKKRKYEYYRIAHLIEEENKKNPNRPPKGIVGEQPSPANSQETQKEEQSPTTARRIRNIQILKDLSWLNKSRLFNKQKIDISCTLQDICKYLGNQAFEQSSLFIQPGKTEELKLNKFLTYVNQQVKKTQIKNDSQHLKPKKKLSQPQEQLIQTLIQKQQEESKSRLGSQHSKQNNELLLQMNKLSKDFNVNFVRNQVDSLFKNKQSNENPQSLTRVDQNEQIKQSANPKSKTIIQHQPKISTQLFLKELKKNVQSIPQLLNKYNTNNIPSPTTNSHNKMSISNQSNIGKLNLKQISKILIDEDPTEQEVKWKNGSQTHRPLSGTQNFFSNRNSPTISRIIQNDLKINKNKQTPTPSSQQANTKINKQSGSQTNRKSSQPNIHIRYTSNQDKIININININDNPELKKQQQSKHKKNQSEGKPLQLNTNLPDTQQEYVCLTDRGGASEFLFKNSIAASCQNSPKTQKIKRVSSGTTTLKCSMSTKNSLIQQMISQVTKQQQKQEVLTSQFRKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRS7</accession>
    <name>A0A8S1PRS7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1270024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRS7"/>
    <dbReference type="OMA" id="FIQYEVK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="106" mass="12712" checksum="76492CDFB176D9FB" modified="2022-10-12" version="1">MDNLILLLKNLFIQYEVKIDEQEFNNLENEMNKVLIQHKERSPFKDITNNEKQAETQLEAYGETIIKRIESVRKQSINNILQEESFPRSSQSIENNQRQHLHYHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSG8</accession>
    <name>A0A8S1PSG8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1280026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSG8"/>
    <dbReference type="OMA" id="NSHQPGH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="154"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="232"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="497" mass="58471" checksum="8DF86B476DB47119" modified="2022-10-12" version="1">MDKSVLLEMQLKEALDRETNLKKLNESLMKAMGDISNHEKGKEIQLLNQLHEQEISKLKTILHEKITILEYEYRNKCQEFNKLEKKYNQLIQDYEELENKEKKCYKCNDFQLQITQIQKQNEIQKEGLAREFSEALSNQKSILEYDYNCVKLKLKQVTFELDQELKEKEKLQKNIQELCQSHEQAQRSLLQQIADLNRVHNETIIQNNQYQISTQTREQTQSSKICQLEQDKIILEKNIQEYKDQISKLENQLKQLDLSLKEKENKLIEQKFELSKKLTNEKKIAEQCQAVALKLKNDFQRKQSILIEESLKKEQQLKSVQTQLTRQKSKNKYYENALSQVNLLEIKNSTPKQIKHKKNNFSNDFHNSHQPGHTKNALSQGHVNKIKILPSSIQKSEADSIHFNLTDMLNSTRNPDQQNLSCRQSNEDIGSHIEQFNCPAKFVQTNEDSVIPIQQLTDRQLSPRTRIQTVNPKGPPQRIRQIHADLYKENLVVRPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSL1</accession>
    <name>A0A8S1PSL1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1290037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSL1"/>
    <dbReference type="OMA" id="FYYEKDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8106002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="391" mass="46059" checksum="375E6521C7F54244" modified="2022-10-12" version="1">MQVNKCMFCDKLNNDGSFHLILTRALYCKYSQSQNFYYEKDINDILDDKQVKSTIRFKDYVQLDEPTEFMRRFYRYFESDDRIPALLEYYKYHINIPRNFHCKVINKRMEKNREIQYCKIKMELGLFEEVKQQTQSKIKDTSEDCSVSQLKYLLRDLKIESTQTDISKISNTTVLRDLVQLIGDTKIQPKPFQIFNQNKLIQKSLKPTIDAQRQMYQNYQGTLKKTIEMQLKQAQQLKSPPLTQRIPKPIQTSRQTSAQKQRSKIEIPIKTIKKPPQLKSRQASLGNIEMTKSCLTTARNTPQQNELLLELAKKMFSTKMTFNNSNQKKKLEKQQQNNFFVKGRTSLAGSQKNFEDLKNSMRSLRSRHLTPSQICSSNHGTPKTKKKMILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYA7</accession>
    <name>A0A8S1PYA7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1360004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYA7"/>
    <dbReference type="OMA" id="VVEIAYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="584"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="607"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="646"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8108091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1139" mass="135023" checksum="C68CF67A7B4E954F" modified="2022-10-12" version="1">MPISVKEQEKKQQEKKEQQQEVAPVNPSKDNEDIKPRKEQQVLSSLLRYCKSRPDPELVWEPHQNKQLMSEFQELIENRFKEVKRSSSIYKYNAKIGFQAFIYLLNILELIFTLSYGSYNEITNDNTYYETCYNLSIILCVLLHSGTWMYTYKFREVVRESFFVQIAYYIFYLIMGVLSYFKLAPFIYYMNRDQSIRQFSFSEINKYLQLSGEDKFRNPCRLFKERTKPVNIFRDLIFHRVALVSMIITMTIQTIPQLFIQGFYNTQKESWDGFNVFSYLFLMSNLIYYLLELQFIVFTTTYRQMQTELQFKLQKILLYSIQETKQLFKSDLNYMGFVKSFYFHIDPSAFSPYQKKRCMIQILYFLMKQKKVKNIEFHYIDLYHEVTLQYLANCFQLIKVESINLLYSNDNNLAQINRIFRGINNLKIEKQTSSNLNTKWEADNIDLNASDEKINEIQFIENPKINTGWQAVQKNQFIKDDYIQLSAEYQALIPNDLRRSLNVNRGISGLIVNQLSRIEDNQQNLLVQIETNEQIIIDAKNQIDSITKRDLYAQSVGNIQFLLKLYDYYEVWSKLNQYQATIQTFWSVLNGSLQIVSLIYLNNEPNMFISALIALTAINPILQMLSFAVFQQRVFKNFSPLRQLSYILLFAIFNFLKIWDIVMICLFLAVKRFSEDVKRNFSGQGYLKFKSYASKFSGSIATSVFQNETVQTDPNDIKQIQNQPFYEAVMWRTNVEEALNKIPQFFVYILSLASTQLDGIWVFSFGQQIKEGIFAVKDLLEVVIKDYFIPALILSTVSVDQFFQSMLYLSSISNQILLEYPKSFQIVSKVKQQYLKEKFTFKINVKTLNFTNYGDLKKQKLLAQFRYVLASINTILEIDQAQRLFCMGAELNDLIRCLKESPLYQIKLNYYLDEVKPEHIPYINAFIKNCPKNLQFLQIQVESSNVQQMEVYVERNNILTAFSYSYFQIIQQYKDKSVVVQQQTLIIDEKFLKLDRYNFEQFYFEVSGNLNLRNCHKLFENFDKLQVFKVSIFNNAEIQTFEFSQNLRSRALNILDITCENIILSFKQYPFNNLTQFKMILKRCEFNKEELKRILQGLNNKTEVVYIDMSQCLQRFTVTEQKDLVRGLEIKKIDAFIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1D0</accession>
    <name>A0A8S1Q1D0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1370154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1D0"/>
    <dbReference type="OMA" id="DCCEAFF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362:SF309">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8108628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="792" mass="91909" checksum="DFA55422197FEF5D" modified="2022-10-12" version="1">MMTGNINPEYRYLSILAKTWNEVYMAENLQTKQKCCIKQVLIDELLRERFQSEIQILQKIKAYPHPNIISFEDSFYYEKVTTQDKAITFGCIVMELGITNLQQYIQSKKQSDPEFRFQEYEVADFFVTMIKAHSHLQEIKIAHRDIKPENIILFNDENLLFKVCDVGFGTEIIDEEQRSRTIGGTVLYQAPEVYRAFKRRKHQAKYNPYKSDVFSLGLVFLLFATTHNMTKQQREELFDDEMKLYNYLREKRKQVKELYPRIKGVSKILKLMLDISPDQRYDFSQLSQIIEERQYLEVKSPAKINHKHLQSIVNLDDFQLDIKNKEDVYLDLNGMQNKSQDELLKFLETIAQQVKNFQMITLQIKLEMSCLNVRTVAPIEKILNRAQKLKELQLHLWNNKLGNLGLLFLTKAIMKMSELKRLTLEISNNLLSDEGINNSLPLLEGLVQMEELKLDFGLNYLPMDCCEAFFSLISAMPKLKKLDLSLESNYMNQVLSKLLKQTVKRLPLLNQLSINFSNIPMHNEGFYDLGESISELENCELFLWGSQIKDFGVEEFAKGLSKCEKIKHLNLTLWNNQITKKGCESLGQVLPKLKKLSSLTIDLSKNRIEDEGVRFLAKAIHEMQQPLRVLQFWIENVSCSSFGLRYVNRFLVTQTKLRNISLNFRSNQIGLDGMELLYNGFGQATQLETVNLILDHNPLGDDGVNTLFKGLCKLKELQSLFLSLESVQGSGNSVTVMLSSIKSWTELKELHVNFLKNDTDLADDINLKNRLLEIQSTAKINIELKTINQQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q471</accession>
    <name>A0A8S1Q471_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1440063</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q471"/>
    <dbReference type="OMA" id="VTCGEAH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF408">
        <property type="entry name" value="OS09G0560450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="75"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="3">
        <location>
            <begin position="130"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="133"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="186"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="239"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="289"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8110456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="392" mass="43554" checksum="DCA86981F232469A" modified="2022-10-12" version="1">MIQESKQNRVCVPTQCAIQEQLQYVTCGTDSTFCITINKKVFMWGQGIQIPKQFELQDIEQIAASKEHWFAWSETQVFGCGSNKQGKLGMNNSVNTPTLFDIFSEASQYGWQLQKIAVGLDHSVALIEVEDENKIFVWGSNTYQQLGFDASEQFVAIPHQLDLDNLPRIVDIYAQHNYTMAIDEEGRLFSWGSNEFGRLGQNAFAKCMKLPSPITSLSNFKIAKLALGTFHVLAIDTQGNLYSWGRGLQGQLGHGNSNDCSKPQQINSLQQIRDVACGEAHSIALQNNGSIYVFGSGQFGQLGLGDYKQQDIPQMLQINGEMIACGRHHSAVLTKNGTLYMFGNNEQSQLGLECGKSGAYQTPLPKQSPMNVLQHLCNLDRPIISLSQLMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4B9</accession>
    <name>A0A8S1Q4B9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1430003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000147">
        <property type="protein sequence ID" value="CAD8110077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q4B9"/>
    <dbReference type="OMA" id="RICCENT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="523" mass="61983" checksum="1A60CBE06A5AF83F" modified="2022-10-12" version="1">MNISSQNNQTQINELQEVTKDQEYFDQAMQLLFRRQFKIIFTKNQEIKIISSNGEIIRSDIILSSCHEDPELPFFNLEQIKHLKWLGKYQDTKKVGKWIATWKNEILLDVGGQYCQKGMKQSRWKEIFKNYFEKAQVYEEGEYINGKRIGIWNTIYQNKLIGGGFYDQQGQKNGQWIELCNYFQCDNQIIYQGEYLNGKKIGTWDINRKRLAIFLNLNGCKKKGNLELIGWEQYDQQGQKNGKWVELSDDFQKETQILYCGEYKSGRKCGIWEIHYQIQSNRPFYLIGCGIYNMEGYKNGRWVEVTKNFLSDGQVIYEGQYKNGKKIGKWDIKVKNDQTFEKIGYGSYDEQEHKNGIWLELSENYRNIYSEVTYYGEYKKDKKIGKWEIYYKQSSNYPFKLIGSGSYNDESVKYGIWIELSDYFSNQNEVLYQGVYQNGRKVGKWDIKFKSHYFNDFHNIGGGIYDNQNLKHASWVDVGDNFSKNQSIIHKGEYNHGKKIGLWEEMRICCENTIFKIGEKIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5D9</accession>
    <name>A0A8S1Q5D9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein disulfide isomerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1450088</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5D9"/>
    <dbReference type="OMA" id="KICAIGF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052842">
        <property type="entry name" value="ER_Co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057305">
        <property type="entry name" value="Thioredox_PDIA6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45184:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_102, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45184">
        <property type="entry name" value="DNAJ PROTEIN ERDJ3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24541">
        <property type="entry name" value="Thioredox_PDIA6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035897798" description="Protein disulfide isomerase" evidence="3">
        <location>
            <begin position="17"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="31"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="PDIA6-like C-terminal thioredoxin-like" evidence="5">
        <location>
            <begin position="529"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="666"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8110789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="729" mass="85417" checksum="69BAA40723755DEC" modified="2022-10-12" version="1">MYKIIFLAVLCSISVALFENSYLIKNANKLQDFRDILESEFVSLVYFYSDQCENCQRAAGLIEKVAEDQEGIINVYGTNCDEINKDPKSNEQIPYCSSDVIQHLPQITFFEPPRQSINPYTNEKMIATEHRFQGEASPQSLGQYGYKFIPNHVIRINTLEELKKFETSDSKLNKVILFTKRKETSPLLKAMNIHYLERVKFGEIVASPETQSLLDEFDIKDLPQLVGLENNGDNTYNREIFDGDLLFKPIKKFVRGLASKDRIPYEQVNEQSSKKQEERKNRRQKQDDQEKNNEKQNVITAIKLDQNILNKQLLRNDKPAFLHVYKETPHKAWEDTIKKYKSLFDYYELQVNTQEDEELIKELQIKSYPSIRFYQVGNTAKKRASKISFTKEYSLDEINKDIQELIDDKTININEQSLQMQLSQYITDNNIVVLYFYQTPQVGLTYRVLSQLQEYHGKYKFLSFKNASQKVIDQFQIPNQPAITIIFRDVKDKKELEEELKPDQVKQALFTGKHGYDEIKSFLDSFDESKKTSNKKVQEIQNQEQLEEYCEKKQTLCYIALLNGEHKQVKHDDILTKWEYQLEVLDKIKNTHGSKQASFVFIDASCHDELLLKFDISDDALPNFVAYSSNKKIYSKLIGRFDYDSISKFIDKQYKGQSSNININSIQIIEKNCEEIFQKRKEASQQSGLSDFDEEILKEILEEERLKKKELEKELKKGKKKNKKSKDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8D1</accession>
    <name>A0A8S1Q8D1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1450135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8D1"/>
    <dbReference type="OMA" id="WEMFIIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="432"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="711"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="827"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="432"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8110880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="991" mass="116312" checksum="090B31FD91FFA547" modified="2022-10-12" version="1">MNQNSTQVPILKHLIQIRRAVIQNFKSKERCRFHMLELLLLIENVEKEFKGVVYDEEFILSIMKNLTQIDSDELMIEILRQSLPFWNATIQMMFVKHTLTILSIICKTQNIQLRVAILQFVMDVNIKTYDPAFRLVVKDGLLKIIIQILKSPKEVKELALIALDIFQPLDHSMSMTHRRTEYIPKIPQPTMGQELPCEFEKIFSSETDISSRLNDSMIQPFDNRNFYIDSSPAYRGTTESQFQNNTLTLTQVVEQKKYNQEIIKYSDDDSLINSSFFGVLNQDAFQKPLQQMDSIYRKLEEPSQFNHNTKNAQTLCDEELRAELQDQVQDLHINNKFINPLQYQQYNKPIQDNSNIMTNFTNNNKYSSMQTYENKSIDSYRLQSNLQQYSLNNEQIKQNPIQYNNNYTKPVETNLMIQNTPPRSRFISPTLQKQIEQQQQQQQFPDFNQNSNNNISKTEKLRINSPDQFETIKITEIVNVDNSQNQSLTSPSPSAPILPLRHSSFVNNKSEPLTNKKNQDVYYSQMVQPLANQKILQRAIVNRAQDNNEYELDAQLQQYSRKIQSPHKNNTFLDQQSELQSNRTSQQQIIIPQPQKQLYSVQSPTKLPQSPKLITKQQERLDQKELFLQAEKAIKFQRTQPKAILPEWQKQSNKKDTKQTQLNNQIYSKNNGNKQDDLQVLCVICEDLIPFEDVDSHSIRCLAKSKQQAKTQNAHTLILNLNQKLEQLKQNIIIQSKQLGDDLINSEEIINCITKIIQCSYNYKKLKQYNELLGQQYQKYQQNFDQKRFIMILTLQRVLAVSQEKQLKLQFVEESQNNQLEDESFLYNELNKLNKQVEIEKANLEQAKLEQELFKKIQKEDLENMKYLRAQYLEQNVDNEVLSQINSEWDVRNPETKTNRVTNSIVSDIQYQRDFDGMAVRKEGNSPTIIQNEDYKKAFYQLAVQIKLNLPHNHPGKNLLLQDAYEKAVSLKVPKNGWEMFIIEEIRNIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8U2</accession>
    <name>A0A8S1Q8U2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1470131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8U2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="454" mass="52410" checksum="5E602D61CD59B21A" modified="2022-10-12" version="1">MDIQAEIIKIKQKFQFHYIKQNISKQYTQKQLKSFLENQMIQNSNIKSPKTIKSKALIDLMERIFRKLTSKKQQFPIVRLEPSFLQYQPVNKGNTARLSYQQLKAQKINSLHKVKHKNGSSYESTLQDGNKFLMPHNPNNNNEKAFTKKNICLKMDIQINETKSIKVLEVQSIQGQSEKFNIQSISAHEMNYQLQYTKILEQIGGVIAGTLGSLTADCIINGIDAQHQVKDYQLLLLFKEVSTCLKCTIFWKSSSLCWNWINSIYDKNLSRRSFIGIEGGMGLGALGGPIRVVIDPISGASDNLLGRATDHFTQFTRWLLNQSRITSQDKLKIVKEKVKILILIAQTDTFIACLIPSIDRNKTEIQENEEIGIQFNEYKYIGYDASCQIITFRLLATTEDEILQQFQNNETNIIDIAQKILNNYDLHSNTNYIYYNLYYRQSNLLLKQCLHTNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9E1</accession>
    <name>A0A8S1Q9E1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1480051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9E1"/>
    <dbReference type="OMA" id="QSNDNCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="164"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8111584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="237" mass="28232" checksum="D60C8675E853EFFF" modified="2022-10-12" version="1">MQSQISGDLEAQIRIANEQISIEQPLMEQETEDRNKEQEEELTEEQKERRRKILEVQLANKRLLYSIHNKDMEIFRVKKEIEDQKQLLAELQQQEQEIVNDSYLSHRALVSYDTYLRGTLREQDRKIYELELNNADISEISARIEKINKEYERNIQPLVVGDFKTQIDADIKSSKNQIDALRNQFKEIVDDIVKLHEKHLDNIDMYSTLQRQLQEQLLANEQLDYELSQLKKSKEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QB60</accession>
    <name>A0A8S1QB60_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1530103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QB60"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="289" mass="33970" checksum="A84B44E0620811BF" modified="2022-10-12" version="1">MSLQDKQTFKQILAEYMGKQYYTSFILQSDIGRNVFVKNGEIIRIEHYNLPYYLKPYQKHLQQIQHLKWRKNESENLENEVFWSVIWNGYKLYAGGASNINGAKVGKWIELFEHFLEKSQIFYIGNYQDGIKTGKWISQFQDTSIGGGFYKENGQKDGRWTEIENYFREYIQIFISCYLITYGGVYDNGTKVGIWSLNCNHNHIGGGQYDINGLKTGKWRELYEYLLENHEIFIEGEYQNGIRGGFFKIILNNDIIGGGEYNQNGLKIGYWIELAQCFNMQQLLILIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QE11</accession>
    <name>A0A8S1QE11_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1570108</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000162">
        <property type="protein sequence ID" value="CAD8114022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QE11"/>
    <dbReference type="OMA" id="TTREQSQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="123"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8114022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="490" mass="57507" checksum="297769805BB8D0EA" modified="2022-10-12" version="1">MRQKSRTVHEGVQNTLQSVDYNECIYQCILTKKKFIGKSPRYIYLFKNHICIGKSPKVQIPERQFAINTDTRIQWQYSKNMLKSITFQVGSHSYEYFGSNEQLREIKQKFALYAFQMKIQDEYQAESVLGKGSYATVLELTNLYNNQQYAAKCIDQQKINEKKNGYRQLMQEIETMRILSEHKHKNILQLNELYIGNQNYYLVMELAKGGSLLSLMKKRSTLFSRNDIRIIMRQLLEGLKFIHSFNIMHRDLKPENILFMSKDLESLKIADFGLAQSCDQHPYTYPKCGTPGFVAPEILEQDSEFARYTTVCDIFSAGVILYVLLVGEPLFEKKDRREQLELNRRCEINFQRFTQDQLDDIERDLISKMLAKNPNHRWKATELLKHKFFVPSDDQIENEIDYPKQINMAILKKSAMPTFSKNPFMQSITRDKALTTREQSQTLVIRPKNCTLIMQTNLQFDKSSQEQINPLEKLTNFIGSFNLIKDEINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QEP2</accession>
    <name>A0A8S1QEP2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1580057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000163">
        <property type="protein sequence ID" value="CAD8114162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QEP2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="43" mass="5282" checksum="B4BBE51E9DE688CF" modified="2022-10-12" version="1">MTHCFFQQELMEQQEFGKLNMAKIQQHIYQFSVFILIIFMQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFV6</accession>
    <name>A0A8S1QFV6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1580060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000163">
        <property type="protein sequence ID" value="CAD8114166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFV6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="26787" checksum="B6B1BCF96505E2AB" modified="2022-10-12" version="1">MILCLKVSKLFYEIFIYNLFQIMCIHNIQITKNINKVQNSSSSQKVLQQEKTPFTFYRKNLIELLFHQESEIQNQVQFVQETHLKKNKFQCCQWQQSLIKYKILEPKLSKELDEKYSDQKIQQRLQSNSTDGSFVFYEEEQFAHITSKERQVFAKEVIFFNYISIKEEKAVRFTTQFIMFELQYFILIININKLFSKSKPEQLKDDRKWNSLKFIITIGQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QG70</accession>
    <name>A0A8S1QG70_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1550018</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QG70"/>
    <dbReference type="OMA" id="YDYEFIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000089">
        <property type="entry name" value="Cyclin, N-terminal domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000183">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="140"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="240"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8113355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="360" mass="41253" checksum="F27ED157C4E252F9" modified="2022-10-12" version="1">MKDMSKKIFEKENQAEVSNNPRHQNKSLTQLVSLTLIGGNRKNELKSIELKNNNYTSISSRPLVFCKPEPKLKTQTTSSPQKQSPIKPKQVETQSNELCKLINNYSEPILDYYIELNNLTPKNCLSNHTVSANLRAKMVDWMVEVLTSYKCKDQTFFFAVKLMDTYLSKTQQKHIPQDLHLIGVTTMFMSCKYEEIYPVKLQIVHEKIAHKKLTKDEIKDKETNILSTLDFNLVGITVLDVITIVLSILNMNQQLYQITLYLAKMALYDYEFINNHTYTQIACAALIVSTKIVEQIDQSLSSEVVIPLIVSTLKIDNSVAMDSANKLLNLAKGFEKQYPNLENLKKFSKFQLSDILKTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QMX7</accession>
    <name>A0A8S1QMX7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0350014</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1750014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000184">
        <property type="protein sequence ID" value="CAD8116584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QMX7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="173" mass="20221" checksum="3526105F1E3B07E7" modified="2022-10-12" version="1">MGLYLEQTDSKKSKRIVQSIVQQKYYTMEMGKSKDNGMEQKEIIGKKLKFFRLDNMQTIKGQDFGDIRIEIKIIQTILLSKFRKLACGQIQVLIILIRIKVTYLGEQNKDKKSRGWKIYNNNSVIRGGQYDRMGQIIGLIYLKISTKRVKSLLVGLCKWQTIGKMGYLFQRQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QP68</accession>
    <name>A0A8S1QP68_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1710029</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000180">
        <property type="protein sequence ID" value="CAD8116347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QP68"/>
    <dbReference type="OMA" id="GFNVFLH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051019">
        <property type="entry name" value="VLCFA-Steroid_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899:SF13">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8116347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="310" mass="35446" checksum="72C3144ABBDDF205" modified="2022-10-12" version="1">MFSEFIKAYPKFQTLLYILGILGLLKCTKILCFLWKLFRPSANLEKYGTGSWVFITGASDGIGKQLAIKFSQRGFKIILVARTRSKLEAVSQQLQTESHIIVADFTQSMDNKLFDRIIDEVGERDVSIVINNVGVDAFNRFHLISDEEVYKTIIVNCMPVTMLCKRFIPQLLKRKSHKSAIINVTSLAGQIPTPYFNVYSASKSYGEFLTQTLSVEYPELEIFALRPSEVSTNMTFNRKPDLMTITSSDCADGLLKRMGQNTNGHWNHEIQAELYKLVPSWLYNWVLIKFIAPQWLKERTSTTSTQKKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QTK7</accession>
    <name>A0A8S1QTK7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2310006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000240">
        <property type="protein sequence ID" value="CAD8118255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FMVTWDD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="181"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8118255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="396" mass="46265" checksum="379C034189E67991" modified="2022-10-12" version="1">MMGIDFSITGETLKQINSQSMNNIIAQLIKFNSECQPFIQLASQNTETLIKCFSKFFQSQEILQDPLEQNDQITTNNTNSEIQLFKYQVINSIKENDYCFSVCFNQDSSIMITGYMDGIIKIFQFNQGNIKILQRLNEHKGIVGCLNYMNQSNSFLSGSLDNSIIIWKIYENNKWYAQQKLTEHNNWIRCILINQYDDLIISGSRDKSIKFWRKDNLWQCSQTLYHHNSDIMSISLNESQNYLISCAQDDNEILISQQQENKEWIIIQKINQQGQRLCFISDSLFIVQPYLSSKMNIYELNHQTKLFNQINELQIKNGSSCHCFFESQYIKQKQILVCKNGFNINLIKINQQAEFKLIQFIDLGTGNSFGRLTADGQFMVTWDDKTEQIQIXLIQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QU42</accession>
    <name>A0A8S1QU42_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T2280001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000237">
        <property type="protein sequence ID" value="CAD8118187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QU42"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="567"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="251"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8118187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="786" mass="92956" checksum="8C0EFF8E388338FE" modified="2022-10-12" version="1">MIEYKVETCQVHSQEAVIAIDESELDLKIRKLCLQCLAKGYKRKVTLIKDSINQVEETKAQIKQDRQCQLQNNLESLSSLVENIEQLKDFYIQKIETTIEFVKKWIIQIQNIEEEFINKMSSHDQNDLQQFLSFIKQQQDIQIKEQPQFQSKIQEILINMKEPDLLIKCKTILLNVHSISTVFEKNTIIEDETNDLIELKLQCEDHPQKRIILLDVGQEKTCRKRLACESCLDIHPSLHYKSITYVHSQWKQVLQKRQENMNKNLESMQQKVESIKNMLQQMQKSINQQFVESIQKLTSKFNQYGIQVDEVQRKINISWQKLSQEDISKIAEELSKIDQPSIFDDPLLHEYTTQDNQVNKIMQDILLSLQESQVTQLNQLNPFIKGQLLSKMEKQLQGTYTGLDQLGYTLKDIDTQTNIKNINLQLQNQEIQYSMQIQKAKNSNFKLEFQTQEIQSQNLLREKENLKQFTYDLIKESSIKQDQTCCAMAFNKDKSIVAVGCDQQIKIFEFKQGIMEQIQLLNKHENNISTLNFMKKTNQLISGSHKDGQIIIWLMNQKNQWTCSQILNGHSKGITCLIFNNNEDLIISGSFDKTIKFWVKQIEWISQQTITDHEKDVYALSLNEKQNRLISCSDDDYIFIIEFYEQDKLWIVKQRIKQINFGIRLCFINDNLFSCQLYQKDQLYIYELNNSNGQYIKTKEFQVRYSQKDGYCLFPQQYIKQKCLLINKNGQYINLMKKNQNDEFEMGQIIEYQTSRIYGSMSDDGEYLITWDEYSKEIQIRKYQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QU75</accession>
    <name>A0A8S1QU75_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2220005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000231">
        <property type="protein sequence ID" value="CAD8118060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1335"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1393"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1444"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1481"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1518"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1579"/>
            <end position="1604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1654"/>
            <end position="1675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8118060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1772" mass="202649" checksum="BCC48F08E6D79C02" modified="2022-10-12" version="1">MDSVYQKQNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNNFLHYQIDDPSESSTSLIQIISIGGLIEINNFICQSNALTNSSNSFISLKSEIIQLTNYSISKHNVIPFELWMSYYDFSIDIDEYDQDQINLIIQQIFKIKSKGGGGLISASSFSCFNCSFEDIIGLKSSVFEIKTQGDGNISFINLTIKSLEYDLSQIVDSTGCITIYSSNSLLNLQIYNAVFQNIFNRMAPSIFTIIPSLQKNQVRIEDIQIINTISLKNPIINLQFSSYDINLNTVIIRNIKISQYEDIWIKYFDKTGMISQSEIQDLTGTSNAMIQLSNCKVVIQNILIQGLYACPLIKLINVQYLFISYFQAIEIQTFYGFSLLELKLNLQIKQSIYFEFVYIYHFKPYIVRDASIFYNPKINYMISGCGQVENIQQNPQIYYFNDLINLMQTWQLSSIIEIQTDSNQTVIYLQNILLQDNLCQDCSNGLLFLKMENQSQIIIRDLKCSQNMVQINGCINIVTNYSQNQTISISNSNFLLNNGTNGIAIYSSGIAISIAKCKIIQNIGALGGGLFLEMNNIHFLIRETIIINNFADKGGGIYFQGQSPINQENFIQSLILFNKANTESSNFFEFPAHLSLIINSQEMQSEEIQISNIKLRRLLLKPYKIIEQGVCKTSSSLMIPSEQVIKDYTIFIPSQQVSLNIFDEFQISLRNSRNELLTNSQLLFCQVSQINLEFNQDFQLDESEIIATLQADYNSYTFDLGSISFHYNPYQEENEYLQILISCSTLESTEYLYYLINARTYKCQLGEFYADEGCQICESIYGFYSVTYNATKCSIFDKIKFANITSNSIQLFEGYWRPNLYSDYTEFCFKNEKFCKGGWMVGNELCSIGRIGGLCEECDTHNIRGEGQFFKNQSDAECFGCSINTITPFILTSLWAIITILITLRSIEKSNQLFFTCKLNFKPNIAKILFKLGQDMESIFIKMVINYLWIFSVIFTFNINFSISLSLIESTSNASQFMAFNLDCFISNTFENELIYLRIIATFLLILIQFIIIQLGYNLYTLLTKGRFQRSIISNTLLYLYVSNYSSLIKQRCFINLWHLESLLMDVCICYTWTNNIWIINSFIFFFLLLNKKGKLAIIKVRRHICYLFNEYKQESFFWEQIKFLKKTSIILVLTFFESNILFKASLLGLCLLVYQLLAGKQQPYIISSLNNLDLQTGQICSIAIFIATAKYVNEQEAENVSSQILQIVIIILFIKLCSLFIFDIFRGYAKKYKVQLFTLIYKIIRNIKPNCMKAESINNVITQLKLKEIRLRSNYQILKMASQEYIKRQKCNCQATRNQNLSSLNRYKQSESVTNKYLLTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QW11</accession>
    <name>A0A8S1QW11_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T3470001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000356">
        <property type="protein sequence ID" value="CAD8119603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="LMIEPEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="152"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="186"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="220"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8119603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="279" mass="32915" checksum="A4703918C5923F2B" modified="2022-10-12" version="1" fragment="single">MGNNIQKEKDNHSASATKHYTRSNELKIAYRTEEAILEINKALNLNPKYAEAYTLRSELYYKMELYDKALQDVNFSLSINNNNAESYYQRATIYWRQKQLNLALNDCWKCVQINKNFAMGYCRLGTIMWDLKEIDKELEYYNQAIQKDSNCYNAFICRGCFYMDGQKADLALRDFNQAIQINPQYSLAFYNRGYFYYKIGEQDKALNDYTKAIQLDPNNVITYRNRGFLYSIIGDKEKALNDYNKAIQLDPNFAIIFNNRGHLYYNIGEKEKALNDYNX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMS8</accession>
    <name>A0A8S1JMS8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="EMGQQPE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="533"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="572"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="611"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="650"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="689"/>
            <end position="710"/>
        </location>
    </feature>
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            <dbReference type="EMBL" id="CAD8043593.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
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    <sequence length="1282" mass="151218" checksum="763D2A7A06BE9BDE" modified="2022-10-12" version="1">MSNTANKNAPAPKLKKESQNQSTIKLAAATLKKQPASQIQKASEPPQKKTANTDSLLEKTSDLAQAAKSTKVVRTTTKSITTKIDDGKLKQQSEQTFKQDFTLNKNSKKQTLKPGKQQEKQKVEQQKDQRRPEGVKYQDDTNSQASNQFDQTQDINNSILDQESILENFNQETTEVNNKDSILNEKQVEQRNQIILSQTENINESINLDCEEDNMLSVKIDDANDDESKSPYKEVNLIEYDDNDSKNIEKDLIISQKQICIQEVQSSNQDKQSKKSLQDNINQFENIQAQQNQQNQLSIKEKNNVMMQDIEINSNQQQESLEKNLNEEQKMVENIDVQISIMQQQQIYKQQDNNEQDGENKIENETNHQDQLQIQEDQEQNNQQSQQQENVLQENNQQDGENKIENEKDQLQIQEDQEQNNLQSQQQENVLQENNQQDGENKIENEKDQLQIQEDQEQNNLQSQQQENVLQENNQQDGENKIENEKDQLQIQEDQEQNNLQSQQQENVLQENNQQDGENKIENEKDQLQIQEDQEQNNLQSQQQENVLQENNQQDGENKIENEKDQLQIQEDQEQNNLQSQQQENVLQENNQQDGENKIENEKDQLQIQEDQEQNNLQSQQQENVLQENNQQDGENKIENEKDQLQIQEDQEQNNLQSQQQENVLQENNQQDGENKIENEKDQLQIQEDQEQNNLQSQQQENVLQENNQQDGENKIENEKDQLQIQEDQEQNNLQSQQQENVLQENNQQDGENKIENEKDQLQIQEDQEQNNLQSQQQENVLQENNQQDGENKIENEKDQLQIQEDQEQNNLQSQQQENVLQENNQQDGENKIENEKDQLQIQEDQEQNNLQSQQQENVLQENNQQDGENKIENEKDQLQIQEDQEQNNLQSQQQENVLQENNQQDGENKIENEKDQLQIQEDQEQNNLQSQQQENVLQENNQQDGENKIENEKDQLQIQEDQEQNNLQSQQQENVLQENNQQDGENKIENEKDQLQIQENKPQNIQQYLQQTANVQQFGNQQDNVYIQAQYQSNQQQKNQITFTFYQYQQQDNNNFLHIQQNKQVIQEDEIQNLDIGSIYFNQVQQSTKQNTIDNFHLSYNTQNLESQQNNQNKVSVYIQTQSTNNDQINPSQNLEQNHHILNISQEDPSLQNTKEIQFNHDYKLGQNSNRFEKYNEIEDQNQTQDMIEQSQFLQDDKTDLLYKNQNLVNDQITDYNLTQVEENNNEQYQQKDQKIQITLQDKQDDYIVDIKQGNQTPSTNVQEKKQQKKKTLICKCCQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP43</accession>
    <name>A0A8S1JP43_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0060126</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OMA" id="RRCKEAC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Fig4-like"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738:SF5">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Syja_N"/>
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        <property type="entry name" value="SAC"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="SAC" evidence="5">
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            <begin position="149"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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    </evidence>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="845" mass="99853" checksum="A5CF17FFAF84B177" modified="2022-10-12" version="1">MNLEYPWDVQKIKFKNIQVFRSHQKEKYILIGNHTLSQQFCILHLNILKGPDLYQKELKQYIYEEERQFETLEDAKQYLNKYNLTYLYTVSGIIGFIKLVQGYYVMFIKRRKSIAKLGKHKIFTIEERSIVELFDGPYSQIESKYKKLLQDYDLEIGFYSSYTYDATSSLSKNIIPSEDVQNKNQKYHQIGQYRNLFMWNHYLLAEFDKIIKDKRWVIPIIHGYCEQSTIKTVANYFSITLLARRSTKHAGARYLTRGINEQGYVANFVETEQIVIELDKSTCQRPACSSFIQIRGSAPVYWYQEPKMYLFKLPIKIHQSDPYLYATKKHFCDLISSYGRQIYMVNLVKQNEHNKREQVLAEEYFSAINNVKDEMQSQTEIEIGYLGFDMKAQLKQDKEEFVNKCYSLAYYCIMKTGVFLFVKEPKCNENVIIEIQNGVVRSNCVDCLDRTNTFQQLIGEMALGIQLAKINQSSLKFNLLLLNESILQEFREMYEQLGDFVSQQYGSSLAHKQNIGQKSKRIELFTSLKRHYNNNIEDPQKQNQIDLFLGIHRPSPDGPFIGERITIFLFPDTKYLQQYTQHKQWWVNPYLEFQQKSLIDEISRRCKEACQQAKKDQKMKIERKQTIFNFDIIEKMKHKANQKLKGKNVFILSSPYTKVREKPPNYVSKPFNLEEKLKKPYYDVIQIYEAEEEGDSPCDSHHSSPKLNSQNLKTRFSLLKGNEILEIEKYTRIKTFDELTDEANQIRKYLKYNLSSDDEEKLRNNLKRYLNGEQPKEQVALIDKNQDIQKQYSEVQKSIANSETVQDISIQQTKKIPVEQKSVKFLESLLKKNFAQSQKLKINKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPH5</accession>
    <name>A0A8S1JPH5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0040563</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPH5"/>
    <dbReference type="OMA" id="SHRICNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:cytoskeleton-dependent intracellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00065">
        <property type="entry name" value="FYVE_like_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947:SF28">
        <property type="entry name" value="GIRDIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947">
        <property type="entry name" value="HOOK PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="19"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="405"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="440"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="782"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1077"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="412"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8043339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1140" mass="134722" checksum="FD380A407BF7F8E2" modified="2022-10-12" version="1">MSRKSTQVLIKSVILSEARGKFKKCQICQKEFGIFHKEHQCKRCRRAVCQNCANHHGPVLGDAGYSKQSHRICNTCKEESESIKKFIEQYKIGIGKDTFSLDWLKSSGLTVDIAKKEYEFAQKENDRAKDKGEFMKMKQELQLAMIDFWLNLTYSFREFLFNLMKDIELDLLQQQICRVLGALLLQYPEIGYGSDQVMITIFLLCFCSEASAFIILTILYSDIIPSYLYPLNLRKTPYDYQHEIDQVLQVLEQGFKIKQSELQNIKPFIRTRISRYALTLGINLYQFQTSFYMINLILLGPKTGYDNFIKCLAVSFYQQFEDIKTFINSQEDAEAQILRNVKSTQCEKDFSITKVIIFIPHKRVTQSVVIQRSNEKKPSNQIQPEHNKDVKVEIQIKTNQNMSENAAVQDKGYQEQRRKSVEVKSTPEVDEEKTSLKLYIEKIEETLMTKHKLVTELQNRIKELQNQTPQFNNNNPDVDKQLIEANEQLRKRIQEYETQISQLEEQNKYSNKLQEDAQETVISIQKSNKELEQQLENSLRTNKDLYLQIKDLKNQSSQLQTPSPLTPQREKNFQINIALVQKKNEEQLQQQIDQEQQKIVQLEFQMNQLNKELLGLQQLNQDKLKIIGNLEVRLIELDSTKRENEKLALEIATLFKKIEHHELVIIQQQEEIDDLKDKYDKLTKQHEIQSEELDQVRIEKRQNLEEYDRKENELLLIIEDLKNRLTEMTKQYQTTSDQLNQSLDHNNQMQNLVQEKDETIQLLETGIAKGVKKIAEIEANHHKQKEDDRKKIEELNQIIEQNNQQIKNLEDNIAEKDAKLEEHKKEFKALKIIFVTLEEKWTKLDNDHKDLSGQHQRLIAECEEWKRKFNEVDGEHQGLKEKYRLIIIEYEKVKENTNEKMNELKSQNDDLHRKLKALEDKHQLLQNQHQALLDRLEKETQNHIKKVGELDLIIIGLEKKAHEYKTLSENQKLEIDNLQAIIKELEGKIAQAEKDQQHRHQQLLVQITQHLEKVTKLENRNSKLELDVLELEKRIQELILYNQDYKNEIIKQDEKITILIKEGQTKESEIIKLIEVIEKQKLDLIDYEKIIRELRQRIQELELIIIEKDKKIVEQEKSIKKLQTLLVQFKTLSEEMASIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPQ0</accession>
    <name>A0A8S1JPQ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0050112</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPQ0"/>
    <dbReference type="OMA" id="HRVTLQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000586">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="194"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8043459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="227" mass="25631" checksum="8E4C842B98EC6A10" modified="2022-10-12" version="1">MNIYNYLFKFIIVGDTNVGKSCLLLQFTDSRFRNEHDATIGVEFGSRNLKINEKQIKLQIWDTAGQESFKSITRSYYRGSIGGILVFDVTNRQSFEDVQKWYNEIQGYACDKIEMVIVGNKIDLEERREVKTEEAKKFAQKFGFDYFETSAKTGENVDAVFESMANKVLAKIGSGEIDPTQEIYGIKIGSIGIQSKPANPPTNNQPSKPQTITTTGQNQNQKSESCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQE0</accession>
    <name>A0A8S1JQE0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATP synthase F0 subunit 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQE0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035941064" description="ATP synthase F0 subunit 8" evidence="1">
        <location>
            <begin position="23"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="10726" checksum="357DA29855CE16AC" modified="2022-10-12" version="1">MNITFNLFLSIILYWAIKTSRQDAGNQKEKRNSLQLKKEQETQEFQGKQDRKIFLLFKLLEEYKLQSLCPLSSRIDQGFESSSVNKVHNNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRS8</accession>
    <name>A0A8S1JRS8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRS8"/>
    <dbReference type="OMA" id="CINLSIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="668" mass="79090" checksum="4E8C109A33373B2F" modified="2022-10-12" version="1">MNNLPFNKWTLSFIDKQYEDLYEVEMNKYRQSYFRIIEIIILLLCILFFIFNLMGGRQIIVMVTLAFSSLVIFLALIYGQKLASKVRYYYFLMYMTSLAVSLYVASQSSQKSPFLYGYSSATIAIIQFLFSHFKLRLLTLLIVPAVQLYILDVFTVQDFGYIILTFMTQFLILLYSHYNEFMFRLAFSYMTYNLKLKDITQEYIPHSFLALSLNVRTNSFQLEFQNQISQTNLNVEDTKTLIEMMRNIYVVPKGQIPTCVDLQNINIQRSGDLSMRRLANKKQTLEEFAFYKIKQNLQDEQQQPNEQNEQMEGVFYNQVKEKNIIVSIDIKTINYGKNYLLIIIKEEKQPQMLSKSEEQIRFLSKVIQYAANQLSASHQHLYNEIIGLKLGSLDTNKFWQIKCINLSIMNHFQNFYFFVNSTQIDSFLATYNKFNIKHFLINLQQHFSYISQKQKKKFVYQLNLDDFMIKVNSKFLSQIIINIYEQCLKQSDSNSTINLNVTNELNLNPLHNNIIQKNCLQELDFIVKVAEDSQESPTKIEFLKLIKFEFTFFSSTQVDLQPDNTLILNPQTYEDFHFNNNQEFQLNHPITNFLLKKIGPYNTIQYSQNIYYDQQCCQQALIVFPSMMDLIQQKTLYQNQISFYIYTDQTQLTQSFIKYAQQKSFLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSX8</accession>
    <name>A0A8S1JSX8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0050384</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSX8"/>
    <dbReference type="OMA" id="FRILIFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045913">
        <property type="entry name" value="TBC20/Gyp8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913:SF7">
        <property type="entry name" value="RE60063P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 20/GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="589"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="846"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8043730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="875" mass="104719" checksum="4843F1B003BB1E12" modified="2022-10-12" version="1">MKLKNIEQPFKTKQFLDECMNTSVKSINVQKQFMLEVHQLCKKFSEDLQHLYKLLSVQVDDEETLSQNTVLSNYILSISHIIKSRSEILFEEAIEPFYQFEANYSHLNNTISKNYGLLLDNLKASNELLKHKAADYYEDQNQTFQAQNEQQYKLSLNQMNCMWEQFFKELPQYYEQYQLNEETRLTVTEQTFVKFIEALEYMFKQPAKITNEKLHQSFEIQREKFKTRDLYGELCIQSKKMIDFMNEPKCEMFTSRDEYFRNKAAILLQESDSSKNSNFKDEDFRFIKQLIEKPQQNLQLLYKIKLIPNLTSKEREEIVSFLLSELKQKNVVQLQSHFQSNFFRILIFKLFKWFEDDFQNQKPSSKLIQQFLQIIYISLSLKSDQGCSLLSKISKKVEIFYSQRLWNSIYEELLTNYHLQKQMIDITLKYVSFLQTPNSKIQLPKEIDQKQDSIFNGLFSTKTRKITQNESINIVNLILIASNLPPQTTSSIIMEIITNIQMKPSCSIPFIELQEANQMLKEFQPQIRQKQLSQKFGKRKNTIYCLIKSLKYLQFNENYLNVICISKEYSKTIKTKMIQYFLVSEQTALLNLQQRVRLWSELLDIEVNYKKIQQDYLSASKKLSREIERSIVVDIKRSFSTDCEQRDQIINQYEIKLENLLRLHAFDNPEISYYQGMNMLMVFFLYVTNLNEELSFRLFRSLLEKLLRDIFLEGLKSMRMHFYILDRLIAIFIPRLFIHWKQLQIESPQFATAWYVTLYTNSINLSQVENNQVCFDIFEIVIARGWTGFYQSTIGLLKFYEKILIELDFDQSQKFLNNLSKTQFYRQQIEQQIDVPYFSNVKNLKISIKKQLITELEREYYQLREKIDQINLNHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXL4</accession>
    <name>A0A8S1JXL4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXL4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="117" mass="13276" checksum="53E4337401DCA742" modified="2022-10-12" version="1">MADPIKIVMCILCCPQWFSYYSFLHNCARTCDITSKIFKSIGSTLWKVLFVLFMAFCGFELVNCVLCIVGCVHCFGTPFQAYQGGSTWVDSFHKMVLEPSCDFEGKISDILGKMFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYB8</accession>
    <name>A0A8S1JYB8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JYB8"/>
    <dbReference type="OMA" id="VQKWEAV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000220">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000249">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="61"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="208"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="522" mass="61231" checksum="6825E48533DB8F1C" modified="2022-10-12" version="1">MLRRDSTLSKQSLEDENQMKIESEVLKIVQILSMESQERTLMQLNYVARRLERSFTYFKKLKSQLREDILLKLIKSLFIERFQQYETVFNQGETGRKMYFVLEGEVSILVKNQDQIDSTINSKKCKLKKFEDVLAIRYPNFRQVATKKQFDYFGEIAIEQRIPRTATVIAKESTTLAVLTYESYQKLLRDITQEAITIRSELIAQIYPFDQLNETSLQQLLHDYEEINLNGGALLFKRMQKADCLYLIISGEVLVQKWEAVKNKEESEEENIIETNMLNKSSKVLVNLGVFGRGQMIGDFEQFLNLKQPTPLLRCTQGVVQNQAKIFRIWQSQFNEIIRHTLGMNWYSNYLNEKYQQKQNHSIPKIKLTNRTIRTQSNITILNGIKDPSYIKISSDRYKIIQNVDEKLPTVPKQSPKRKLQLTIDYKDKNNLKYYNAVTPNLQNKPQLNTNQNFSMKFFATSLKQRILDQKNEENSLNFGSTKYLKTEPSSELNIPSCNTILLSPRYSKLFKKETSFHRIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYW8</accession>
    <name>A0A8S1JYW8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cathepsin L</fullName>
            <ecNumber evidence="10">3.4.22.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0100088</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in extracellular digestion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.</text>
            <dbReference type="EC" id="3.4.22.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.15" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JYW8"/>
    <dbReference type="OMA" id="CYIANDA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000104">
        <property type="entry name" value="Cathepsin L 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035717223" description="cathepsin L" evidence="12">
        <location>
            <begin position="23"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="32"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="112"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8045910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="320" mass="35178" checksum="245AB844AA4AC1BA" modified="2022-10-12" version="1">MEKTLLAIGLLMLVGTLYNVQSVPEVQLATQFTNYQATFNKQYSGSELLYRLSVYQANLADIKARNEKLGREIFGETQFTDLTDEEFAANYLTLQINPDDLEVPKAEFNNVNATPIDWRTRGAVQYVKDQGQCGSCWAFSTTGVLEGFFKVTTGELPDLSEQQLVDCSTLIDFNQGCNGGMPSRALNYVKRNGLTTEDAYPYTAKETGSCRIKGGPYKVNGSTAIAANEAAHQAALQQGPVSVAVKASDWKNYKPKSDDYIFPDSECTGQVNHAVLAVGFTSEALIVKNSWSTKWGVDGYIYLQGGRNTCSVWDNSVVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZB0</accession>
    <name>A0A8S1JZB0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZB0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="140" mass="16497" checksum="05D84ED91835E19D" modified="2022-10-12" version="1">MIQIQVNNQININSLKQETQEVPIINEYEEDQPQIIKLTQKYLHEMRINQNQIYNYKKNFENENDFIAINFDFTQVELYQDNIQTQLEKADQCDGFELIVDCETCYGEIGLKVVYSLNDLQGNSKSLKLVISIVDPYHQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K257</accession>
    <name>A0A8S1K257_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cilia- and flagella-associated protein 58 central coiled coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0110183</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K257"/>
    <dbReference type="OMA" id="GRNECCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049270">
        <property type="entry name" value="CFAP58_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32083:SF0">
        <property type="entry name" value="CILIA AND FLAGELLA-ASSOCIATED PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32083">
        <property type="entry name" value="CILIA AND FLAGELLA-ASSOCIATED PROTEIN 58-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21771">
        <property type="entry name" value="CFAP58_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cilia- and flagella-associated protein 58 central coiled coil" evidence="3">
        <location>
            <begin position="405"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="118"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="427"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="658"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="743"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8046950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="890" mass="106249" checksum="ECC9A1C944A5D7BF" modified="2022-10-12" version="1">MAGVSEEGLSLAAMNKEINEYSTDPYIILQIEWDFEGVVRDFSVDPDLELFKIKYETFYNSFKESYENEVRWFKICQKHNMEIVFYTYKVQMYSKMNNDDNQQSETFQKELEDKWRKIEDLKIKEKELKERIVQEKIEIESLKKQCDRVVEDIEEQSQIQLNDKKILIDEIKKNFQSAKSKFEYLEQQNRNLKPDLDNEEKQYYDQIQKIGDLEDKIAKIDADSKEQEKRKKKKELKMADIKQKIDQAKKEIEEKQSKIHDIKKLIVSTNITILTKIKEKESQHTKQKQADDKYAAALKDRQNQEEQLKSHIEQIKIQQEQLEKLHIEIQKEKKDVKKTHKEQEIAAKEKNRIIKEKEEQLIEKNTKDILIETEKIELQQKKDILDKINEMNSQRIKLRTQQQKTLKKEEDVQHQTDEDIVNKINKLKKVENQVMGYEASNESINKMILQLQREQEKYGIEASQAHAKFYQTVEEVKIKNNQIAEQQKQIAEAEARLKHQQQLYETVRSDRNLYSKNLLESHNEIQELTKKYTRMKHQVDQLKEEIKTKDQQLVKEDLEFHKVEEENTKIEQDKAKIEKNIKVDEDLIKNQESHISRLKNIIQGAQTEKQRQQKDYEMVVNERDILGTQLIKRNQELQLLYEKIKQNQSSLSKGEINFREREIELKSLKVELTNLKNELKSTQDQTACIDELRKEINILQKKLLNEKNKVKALSDELENPMNVHRWRKLEATDQEKFERILKIQTLQRRLIAKTEEVNEKENLIKEKEKLFMELKNILSRQPGVEIHQQLAQYKESLKENTTKMKITLSQLKQSQDYVKVDMLKFEIDRIKGDLQDIKKKYFQMRKAKDNDLLQNDQNGDQEALQYNGGKKQNDLQSYALFGVNANRIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3K5</accession>
    <name>A0A8S1K3K5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0140215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3K5"/>
    <dbReference type="OMA" id="HFFSAQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8049889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="335" mass="39614" checksum="57EEBEC64B98AF3F" modified="2022-10-12" version="1">MSTYSLYPVMHEANTFEIFSMLLQIGLDRANNTKTIQQRINQTEHIKTPTDFLSFVNINEKCLSQSLKQEKQRVEQEFALIEMLVPNKSKTHLMSFYHLSLKLITQWLPQFQVKVNLSGEFLDIAELYGQFFIASGMNHRLQQIQAIKKNNLYDKIPFMPLLMKWLHEYCRSQFNKLFASLYETISQMKSNYRQIVSKPKEEIFSPRVNKMPINRPTSCIINKTRSISNYSIRKTSQVVTKRNNNQSVSKLINLISDVIDIDLTHYDQQTHKKKEQIFSTLWSKNNHVRSTHEKYQQTYGIQKPQIQTHFFSTQNKRPTSFFKHSFHIDNNLSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAR8</accession>
    <name>A0A8S1KAR8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Intraflagellar transport protein 140</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0140279</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YAQFMES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030991">
        <property type="term" value="C:intraciliary transport particle A"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002222">
        <property type="entry name" value="Conserved WD40 and TPR repeat-containing protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000015">
        <property type="entry name" value="Intraflagellar transport particle protein 140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056154">
        <property type="entry name" value="Beta-prop_IFT140_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056155">
        <property type="entry name" value="Beta-prop_IFT140_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056168">
        <property type="entry name" value="TPR_IF140/IFT172/WDR19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056156">
        <property type="entry name" value="TPR_IF140_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722">
        <property type="entry name" value="IFT140/172-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722:SF7">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 140 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23383">
        <property type="entry name" value="Beta-prop_IFT140_1st"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23385">
        <property type="entry name" value="Beta-prop_IFT140_2nd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24762">
        <property type="entry name" value="TPR_IF140-IFT172"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24760">
        <property type="entry name" value="TPR_IF140_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="IFT140 first beta-propeller" evidence="7">
        <location>
            <begin position="18"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="IFT140 first beta-propeller" evidence="7">
        <location>
            <begin position="152"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="IFT140 second beta-propeller" evidence="8">
        <location>
            <begin position="406"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="IFT140 second beta-propeller" evidence="8">
        <location>
            <begin position="583"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="IF140/IFT172/WDR19 TPR" evidence="10">
        <location>
            <begin position="756"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="domain" description="IF140/IFT172/WDR19 TPR" evidence="10">
        <location>
            <begin position="1142"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="domain" description="IF140 C-terminal TPR" evidence="9">
        <location>
            <begin position="1271"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="697"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1103"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1411"/>
            <end position="1449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1111"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1411"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1434"/>
            <end position="1449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8050016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1449" mass="167812" checksum="06DB19D25A01221B" modified="2022-10-12" version="1">MSILSEHTITLKNETITTISISNSAINPFIAICTTSRVILYNDQGEKLDYDFTHNQKPTAIVWHPIQPQLSIGWQNGTITLWSEDTRTAKEESAVHKEEINLIQYNTNGSRMVSADITGLVVVWRGITPMCTYQKEGIQTICIFADLNNIIKAQNLFFFGGKSGLVCLADDSKHCSEVCKVVGSIKCLMIYEKYNSVIIITSGLLLVQFKISTSEKTQPDKKVKLSIAGESESLQSVWIGQCLIAISSNENMIRMLQLEADENYVITLHEYQELYPKDKILNDKITCIQYSKKSKNLIAGTKDGKLLFWKNFASTDESPVESEQWKTLKIINHNKPINEIVIGKNSGLIAARSSDSVKIVQETLVHGKIQDTVRILQVDFNKILIYFKNDQQNQDKGVWPMFLWDSKYPIKSIDCTQQYLLIQSANKIEISEFTNNSQILQKSSFDKKCVKAALFQENLILFYEYKFEVTNFKGVQKYFVDFSESDGAIANYEFNNKTMVIWTYNNYFRLYDFSRREAQLQGFNCKFENDKGSLGTIKQCAVNCDGTNVAIIADNHGNQNDLFFVYNSENNNFQSFELPKYRKAIQIMWDHSDPRFFGVTTICTKIKTNSENDVEEEQTDFRAKKFFTFFFNQQLGIKEQDCYILDEKQEGVLSIRIPYIYVIQIKEIIVNDNNIGQTKDQNTNQDNLKNDSNAIKIKEQSNRNQDNQKKNDNNVQRKEQSYRIQDILMNDFTGLERADEPTKRAVINFSYHLSCGNLDEAYKSVKAIQNPTVWEKMAQMSVKTRRLDVAEICIGNMRFARGAKAIRETKKEPQFEAQLAMVAIQLNMISEAEKLYYQCKRYDLLNQLYQAQGQWEKAVEIAEKHDRINLLSTYYKLAKQYEISKEWDKAVQYYEKSGNALKEVPRMFYEADELQYLEGYVLQKKDPVLYKWWGNHLESEEKYEEAMKFYLEANDALGIVRLSLHQDNLKYAKEICNEKNDPAAHYYLAKYLEAKNILPEAIQFYTKAQYYSEAIRLAKEANLISDVTAISLQAPQLIMIQSAIYFEQKKIIDKAVLLYMRGGQLQKALYLAQKEKLTDYVKKINQEISLKKQETVDLTFTNTGSLKETPSQQKLANQQQQLQQQSIQSKDKTPPPGSLQDLINKKQFEKVLETCDNQGLQITDDLVKQMLDGLDDKRKKEVQLLIAEKSKKMGNFEQASKMYIQMGERVKAMKALCKLGDVEKIIAFANNARMAEIYILAGNYLQTGDWHKTPELMKHIIQFYNKAKAFDHLANFFEACSSVEIDEYRDYNKACAALKEAIKYSEKSTNQQKTQQLQQKLKLISEFIQAKQKSPQEMIQLYYKLLNDSNIEYAVRQGDIFAELIEYYYAQGQYEKAYEEIQKMQKKSIVLNPYLDQELIQKVMNYMKQQVGSQQQQQKQQQNKKQPYGKQQEQNDDDFQEEDVIDDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDS6</accession>
    <name>A0A8S1KDS6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0210152</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDS6"/>
    <dbReference type="OMA" id="FDHVDSA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035747767" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="262"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8053870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="41720" checksum="FA059A3D03A2A8E5" modified="2022-10-12" version="1">MSLIRFNILALTIGVCLSAMTPQASLLMKSFHQFGQTPYSDQLKELVQIKLETGGQVDEVLKLIQELLDSLKQDQVDDDVAHSRQMAVFDQNINELEDDLSRLNTDLANANVLIQTLAELLVILRETILTYEKQLAILNEQEQFIRNARAADIKAYNRRVEQANKVINALNLIIEKLSRAVDQQTSEQDRQAILTQIHAECHEQFGPNHPVTILIKLTTKFDVPTVQRILEKLVQIRDGASKSLSEDIAAEEVASQNFDTSMSEIETLRKRLSTDLENLNQQFNDKLNQQKIVLAQKEQLLIDIPITEELLQLTKEQQEQYHQAYVSRQTQRQSEIEVVQKAYNLVFDHVDSVKKSEDLTAKFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHQ6</accession>
    <name>A0A8S1KHQ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHQ6"/>
    <dbReference type="OMA" id="LVEYQMN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="255" mass="30404" checksum="EE5798DCBC04080E" modified="2022-10-12" version="1">MSDRSRTISLRKQQILEKIQYNFQKIDPAFQVKYFIGENSNLVEYQMNKWNRQSRQKTVYEQQIERAIKTLRHPFTLESYVDKPQFITPSSDKNHKEIKQNEEKTQIAKSPIKEETLTRNCIATPSSPLKKLFTQYQDLVKVQYITSSEQMKKLIQQYSDSEKPYSSSRNPEFTPSRQINLKRKKKGTNQFSLIPPSPIPRNREHLLQKNTNNLILLDELQDDLESFRKSLGEMQKFKNLFPKRQQKKSKYDFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KI86</accession>
    <name>A0A8S1KI86_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0220253</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KI86"/>
    <dbReference type="OMA" id="HILMEMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000003">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="122"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8054879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="241" mass="28274" checksum="4478C4D8F58D7A2F" modified="2022-10-12" version="1">MSYQFGVNQLQSTTVPNAKYKTILCRHYQATKQCAIGSKCQFAHGIEEQRQMNDPMPASALSSITTTSQITSIEQQQKNQQPFTLQISLTQFLQEWFRMSICTWYVLIIFYQIDSDIQTQTASQLQQQQQQQQQQQIQLLQQQQQQQQQQQQQILQQQQPVDPMSLTLSHILMEMQQIFNQEDLLQKIRFAIEQIRQGNLPLACDQIRSIINSSDRTADEIQQYTLLYNQSVAYYKQLLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJE8</accession>
    <name>A0A8S1KJE8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VASt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0220237</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJE8"/>
    <dbReference type="OMA" id="IMQTNQS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF4">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="4">
        <location>
            <begin position="145"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8054847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="410" mass="47302" checksum="2917C83EBA6E156A" modified="2022-10-12" version="1">MSAKDLLIQLFGLPKGEIIFQDYSCALKGLISKYGRIFIAENHICFYANLAGSKTNLVIKLDDIAKLDSKNKSDIDITLKDGKTFCFNGFHDKDQVYNLMNALISGQPLSNQTTFQTTTDSAREDDSQIENAEVEIQFLQSGASMDQEMCKFTFSFNLDKFFEFFLADDALVYSIADHRQSEKDTDIQLTKWTPIEDNPTMFQREMKNVIKLTGVPFKDKSKMHKLFTYKREADKLIYTCTTHTLDVPYGNCFQAEEKWEVSQLEDNKCLLKIFASVVFTKSTMMKGTIMSKTMSGLKEDYEKWINNVKIRLEAMAKSQKSQVSNINHEYEDSKKLDDNILNKIMQTNQSQQQKQNISQSQFYQGRKSELLYLLSIFLLIIIMLIQIGILNKQSQKLENLEQLVLKLQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KP90</accession>
    <name>A0A8S1KP90_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0250253</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KP90"/>
    <dbReference type="OMA" id="LQNQYLM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000003">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25427">
        <property type="entry name" value="zf-CCCH_UNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="186"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8057079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="225" mass="26155" checksum="1161615CE3F247C9" modified="2022-10-12" version="1">MYSQGTQYPNSKYKTQLCRHFTQNGVCALAIRCQFAHGPQELRQNVQQPQGFTEQTIQPNAYNKVQGINPMIVNYKTQLCKHFNPQTGQCKNGPTCTFAHGENELNTMPHLQNQYLLMQQQMKQINQQQMQAELTQQILVMILTNMEHIFPGQQQILALLKQGQEKAKSGDTQGASEIIKLIIHDQDRSKEEKQQYQQIYNNAQRHYDQKLKDIQSSQQQQQQLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRM2</accession>
    <name>A0A8S1KRM2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRM2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="152" mass="18237" checksum="95D8321C1E838151" modified="2022-10-12" version="1">MNVIHLEVMIISKNVKKHLKNVKDLKVVHIANDKIKMIQKEISLIMVLLLLLCKFTKTFWFIEMEYIKSQNEYLDFMEDMLQILQDGENKMYINIGLFKTHGVKVGELKQQLNLVQMVLQICLYKHYQLFIKQYNKLFIFFEVNNSNKSKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KS46</accession>
    <name>A0A8S1KS46_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KS46"/>
    <dbReference type="OMA" id="CLSHHQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="256" mass="30587" checksum="D184876A174229B2" modified="2022-10-12" version="1">MYYQENYRKARQFLHQRQKLIDFQPCKLNQPQITTIRQILEVIRSKERSNSQFKYIKTTEQEKQDDYQKPQILISSNLEPKDHNQSLASKLLRLNKITRNKTQVTPISYNFIDRIIEPLKKKTEIQSEQNSPSRIRKIIPCIEYQLPTQLIFQDNQKQVNNIIERKFCIQKSRPKEIINLQQPQNKSIQNCLSHHQIINKEKASPTLRRLNKTSYQCEQVKFINSIQNQKIEKQIQQIRKVSGWTIQSQESVQTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSP8</accession>
    <name>A0A8S1KSP8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA-uridine aminocarboxypropyltransferase 1</fullName>
            <ecNumber evidence="2">2.5.1.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DTW domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0230310</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine (acp3U) at position 20 in the D-loop of several cytoplasmic tRNAs (acp3U(20)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-amino-3-carboxypropyl]uridine in tRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62432"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:16092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:82930"/>
            <dbReference type="EC" id="2.5.1.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TDD superfamily. DTWD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.25" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSP8"/>
    <dbReference type="OMA" id="VNAWGLN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016432">
        <property type="term" value="F:tRNA-uridine aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005636">
        <property type="entry name" value="DTW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051521">
        <property type="entry name" value="tRNA_Mod/Golgi_Maint"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15627">
        <property type="entry name" value="NATURAL KILLER CELL-SPECIFIC ANTIGEN KLIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15627:SF8">
        <property type="entry name" value="TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03942">
        <property type="entry name" value="DTW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01144">
        <property type="entry name" value="DTW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DTW" evidence="12">
        <location>
            <begin position="54"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8055696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="270" mass="32575" checksum="EE82317327497373" modified="2022-10-12" version="1">MHIRDKYIDRQNYKDSDQISNEEQYKRANEFFSQFPKIDLQAEKVRCPKCNLLHKVYCLKCGELLVNKELIPKVELPVNLLILHHNKEKHQKSSAIPAKFLSDQVEVKDFPSEFEFDEYTYVLYPHQNAKYIKELSQEELQNIKKFVAIDCTWHQTGAILEKISNKYPQQKFIKLEDYQTYFWRYQHHSLKCLATIEAIYYCYQEYSKAMNINQNYNNLLFFYKYTFQQITDSVQQEVGNSNIYQKIEQKKQRILQSKQDAKKEKLFKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KW06</accession>
    <name>A0A8S1KW06_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0230061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KW06"/>
    <dbReference type="OMA" id="RMNDKFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="376" mass="44535" checksum="A9FCE9E632DC3844" modified="2022-10-12" version="1">MHNNNSFFQNDISQQQIDQALLRLNALLKDEPPKLEKQKFKKPILINQEDSSSIVSSQPNYTQQTKMTSEQINHFVDRMNDKFKQTQNKIIELRKDKIYSPIKVNEKSQSILKQKIQNGEYQSLYERAKIRQIEKQEKIKENEKQKILEEMMTIKTKCSDSKSVDNFLQKMDYWVEKKTLYIQNQQQEKIEKELEGVTFKPNLNPLSTKIAKQQNKDIDILERFQKSNQNKQMNEQRLLKDELKKTPFTPKLNKNSILINNKAQKQQQFKNHNYFLNQQTLNQSKESSLINFNSINQKQISIDTFNSKQSKSLTPDNVRSKYSTEKIKEDNSIKKRSTTPLKQNKIIPNVQEIKYNPNLKFLMTLAQKELNYSSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYZ5</accession>
    <name>A0A8S1KYZ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYZ5"/>
    <dbReference type="OMA" id="QCWELEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="430" mass="51335" checksum="3287216E71EF3D7A" modified="2022-10-12" version="1">MQENRKQILKSQTSDRSIKNAANTITKITNKKIQQLAFLTQFPQQRKYTVVEKVNKLIDKTQSKLKNQQIPKDVQFYKRNMKKFENLSSFINSKGLLSANFSMPKLRVKEKVNEQEHEYVDYLLAKNYKLQQERQRRKSKQVKEDYEQCWELENSDYDYESPINVGKELKIAHQDQLKGEIEAKAKLWEFQQIRKYKRKITNIELKSSLRSSQHNVEDNDEIVEDTAHLNALHYKEQSDKVIKMIWNAAKKIYNKKQDQIKKEILMHQIAKNYRQKQREMAKEEINKILITGQKQHLTFAKTVRHLPENGDSKQNTEQYFRKKGQTSRKIQVPFSFSPQNLQEDIKSNRKITQINSRLHDIVNQIDNIPVGDQTQTIRKMQSEQILYQTLRETLQPDQIKDTLKGLFLPTDHLYKRRLQYNKKHFQNQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZA8</accession>
    <name>A0A8S1KZA8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0300054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZA8"/>
    <dbReference type="OMA" id="NTMHNLN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051266">
        <property type="entry name" value="CLCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579">
        <property type="entry name" value="CALCIUM-ACTIVATED CHLORIDE CHANNEL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579:SF43">
        <property type="entry name" value="ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="142"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8060277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="488" mass="56700" checksum="A3ABF4C91003AEB6" modified="2022-10-12" version="1">MQIPSTESAMSQDHNISKWRSYGLLETDGGSSSFSFQQKKKKPIQKSNTMHNLNQRIKTQTQDNNDLKNSQSESKQRFSQRTQQSIPSRSSINKQPLDDDIQFDMFSVNPGSNILNLGQQTIPIVLQLRTKTTEELDQIGVDLFCLIDISNSMQGQKIEYVKQILHSILTNLREQDRLCLISFNNEGKLLTGLQKVTPETQEYFAFVIDDLQCIGTTQLWKGTEVAFDVINQRKNKNNWARILIFSDGQDEIALTKIRKQLEYNYDIFTIDSFGFSNSNANKRLSAITNLRFGKHYIITDEQQVFKCLEEAFANFPFNLWDDVTITISTNSQNIPFDKIMITEIHSEGWVELQNQHQYQITIPNLEIGESFLFPIELAFQKFNDNIIDKSKPVSLLKGKIEMQNSITGKRMIKNVDLDAIFLYENDSNTLDFNTYFNCKQSPLVIWVCDQQQTQFNRKSYFELICENLQLKWQLQQQRIKQLYDEEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L199</accession>
    <name>A0A8S1L199_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2'-5' RNA ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0290262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L199"/>
    <dbReference type="OMA" id="RRWMPHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37474:SF1">
        <property type="entry name" value="2'-5' RNA LIGASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37474">
        <property type="entry name" value="RNA LIGASE/CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13563">
        <property type="entry name" value="2_5_RNA_ligase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8059935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="23280" checksum="C2B3C90AE055441A" modified="2022-10-12" version="1">MDQQIKKVFHTALVLVPPKHCIDQIQLLRKQHDKAYDRWMPHVNLCFPFVDPAQFEQVFQVLQNHLKDFPSFQVRLREFNHFQHGKNCVMWLKPESENDGIQKLYQEILKVYPQLDDLNKKSEHGFQPHITVGQFGINQIEQRKKAFQPQFHEVQFQCQEIHMISRNGQDDPFSIIHTIKFKTDNPQAYEYPGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4N3</accession>
    <name>A0A8S1L4N3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0330102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4N3"/>
    <dbReference type="OMA" id="VIDYEIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="518" mass="61599" checksum="CC1691FD16D53588" modified="2022-10-12" version="1">MNELAQINEYIKKIEELYSESFFEKRMNASESEEKNNEIKFMMEQINQDQGVIENLIYISLFLIGTLQAKQDDELENNDFQEKQRKQLISQLQQERDEIQQYKNLYHEKCQIIDEQLNVISDLEQQVSILKNQNTNLENQNLRIQRSQLDNGSITEIIQLQEQQLVKNQNYQQELQALNQKLKSDLQAYQIENNDYKDQIEELNQNYKQLRFELSQLKLQKQQQDILIQSLEQDLKIQSEIVIDYEIQIDLLKEQLRSINTPQLIQNEKCIEKKQEDQDNNLQSSAYTTNLEATQFSDIQHFNQQQTQPNLKLQLPLLSYRQQLYKFQIPEVSERQEQSDQTQKGNKIESDVYTVVSTFEVNQVEKENLRDKTKSFQGDNLIESIPNQQFISQTARLDLNKTQIPKFQFSDINNRITVAKQRKSKTIEQVGVQQYLQQLGKRSNSKLIEIPKSSPDPYQIFFELLVQCVKINSENFDEIYSVNTDNLFKECQKEGKPYFEWQDWIQKRLNIEQNWNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LA01</accession>
    <name>A0A8S1LA01_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0340175</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LA01"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF3">
        <property type="entry name" value="KELCH-LIKE PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="3"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="76"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="144"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8063151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="636" mass="73693" checksum="D9439641D0B9D964" modified="2022-10-12" version="1">MECPMCWELYAQNRVARNLLCGHTYCSICLESIYNVNKRIECPLCRTKHEPHVKPHLLSKNYVAMDLASKHLEVQKKFELCPIHIKLPLQFFCEDDQSNMCTECIAEHYGHKFFKYEHSVRIQSKLKTQFEILEKQIKGFDNCKEQLQLENVKLMQQLDDQFDRLIKKIELRKQELKDQQLKIFTTECEQIDQELAFNQSLLTNMASLTTKLEQKQGELKGVKALKNNDLIKEIDDLEEQVDRDVAQQKQLKISEIKKLPKLVFDLKLINEISKFGQFKKDVSNPQICFFGDKHKILIYNIENNEWTYKQMPNNTLEYNYYAAAVSLPSGDIVITGGGVSRNTMLISPSKGFQSQALKSMYFPRKEHACVYLDGFVYAIGGYDGSAKQMLSCCEKYSLVSDEWKIIDPLQKQKCAFAAAAAINKFIYVFGGFDGRERLNTIERYSVKENQWKVLDVKFKQGFSNAAAISYDDNKILILGGGSNQGFSYDLQVYDVNEQVVKTLSRMNEGRDLRNKLIIFDNNLFACGGNNQSIEKYSLSQQVWVNLKSYDQLVQDNLDSWCSALTFEINSSTSVSNLLKHNQQKQQQYSKQIYQKYKFEEQVSFDNDSFNQDAQSEELEDLEDYAYNQQVGGLFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LC16</accession>
    <name>A0A8S1LC16_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0350159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LC16"/>
    <dbReference type="OMA" id="VRTHWKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010516">
        <property type="entry name" value="SAP18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13082:SF0">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13082">
        <property type="entry name" value="SAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06487">
        <property type="entry name" value="SAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="157" mass="18568" checksum="202A3B02E3E5F849" modified="2022-10-12" version="1">MAQNKKDQIKVDGIHGRYGRFRDKRNQNDKDRHQVPFLVRTHWKYGQITLEEFNKNQTQEILLYLFLSSKLRDIVDQLKYQLDGVYRRDILFKVSNIYLDQHGVQKKKDLGIVHSVKSGKDDDSDLKEHGFKIGDILMVEIETKNQNKQPISQIVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDU5</accession>
    <name>A0A8S1LDU5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0380182</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDU5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665:SF9">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8065930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="29303" checksum="D3F1984C694FEB63" modified="2022-10-12" version="1">MESPKQRQEIDEHQNQVQQPQTHLIQLDIEETNQQPVKRPAPKTFGDLTIIFIFETIGTGLFAYGIVASNGSDFLIAAYLYAAIFLTCKFTGGHVNPAVSFSFYCDDTISSWTLRIYWTAQLLGAVGGAWVAYLILGVVTSPSIKSTNIEWMLADLCGEAFGTFMFVLFIHIQVHEQTRQTNNDIAGIAWIALALFFSRQLSSHSGGCLNPAMGVGLELFEAFWLNDTTKLANFWVFLFAPLFGAYIAQIFYNSIYLPLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDU9</accession>
    <name>A0A8S1LDU9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDU9"/>
    <dbReference type="OMA" id="CLRTPYP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="137"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="208" mass="24093" checksum="71DED98D083A3D64" modified="2022-10-12" version="1">MLPLSQNIYHRQSTSLLQKHFETRKNSPVTIIRAPALPKRQPLSGSSLCLRTPYSDVKLRMFTQQTEESKFDSTMKQDDSQNQQLSSFIDIKIDKLSCTKTTAAESVEEEKQQVKKNFAKQSYLQMQKQLLDRDLELLQLQRTIKILKDQNKKLINENSVLKKENTHMKTKIDSLEKRIEGSPTHARISEYISALTEVDKIKIESLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LE83</accession>
    <name>A0A8S1LE83_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0390058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LE83"/>
    <dbReference type="OMA" id="NRNQSHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="289" mass="33335" checksum="9CC760E60726CCE9" modified="2022-10-12" version="1">MKTFDNAIKPTQKPVCYQVQGQLNKYKSTTKENNSIIQCEELFQTIQTDTNIISEVPDAKIISQSNMPNSIKMTLKSHEIQELPIVQENETHSQSIFKYLQKDEQSQKLSTNTNSQHSNTSKQTQEIFNNQCNRNQSHKSNQPSQNQSQQNKKKDVSPQRSTSPYRQFLQRPQQNQVSTQQRTKSPFIKNTGTQKLTPRTNQRTTPSASPFRQAKENNISTINQRNLRTDKSQNKLIQVKIKETHNQNQSHVIVDLSKCPTQRVLMKQDSNRPKMKVELSLKSLIKNGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LG42</accession>
    <name>A0A8S1LG42_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0370263</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LG42"/>
    <dbReference type="OMA" id="NIIAMIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641:SF19">
        <property type="entry name" value="NEPHROCYSTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_6G03870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="95"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="201"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="256"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="309"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8065421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="362" mass="42350" checksum="DD19C1C85F219107" modified="2022-10-12" version="1">MDSIRERLLQLDQQAQQFQQQNKYLEALNVFEEMLMIKKSAYGEDSEEYFKTSDKLCELCNLIAMIFLQKEKFDASLEFLKKADLLAQSSTRYKAITYNNLACFYRRNGKLRSALQYLQQALEIEIRQETAPSLADTHLNMCAVLSQLNRHAEALEHALISVVLLQDEFLMKPPDQKMEQSQNQDQKSVEQKMKDRVAVLAIAYHNLGVEFEYLKRFDEAIQTYQKAVRFGELHLPQDHQLIGNLKNVLGNAQEQINQQRAKDSNKRQQKELQSRNQNKRKLVEVEATYKQSNKKKTEKTVQHQQQQQVMGQTIRSNPQNNSVNIREDDQEQQGGVQQESSYNDHDELQQEAQTQLQQQQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LGX9</accession>
    <name>A0A8S1LGX9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">guanylate kinase</fullName>
            <ecNumber evidence="2">2.7.4.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0400253</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LGX9"/>
    <dbReference type="OMA" id="HFFEHVK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:GMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd22973">
        <property type="entry name" value="DD_CATIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000776">
        <property type="entry name" value="Guanylate kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00328">
        <property type="entry name" value="Guanylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047501">
        <property type="entry name" value="DD_CATIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117:SF13">
        <property type="entry name" value="GUANYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117">
        <property type="entry name" value="GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="7">
        <location>
            <begin position="74"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8067368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="264" mass="31046" checksum="C8464C2041737F3B" modified="2022-10-12" version="1">MDQQQFDSELQTYFLHIKHEIKQEHIEYLKKHPELNQILNDFTSQIILEKPDNVYQYAKEYFSFFNQEKDLKTCKPLIVSGASGVGKGTLLQMLFKQYPQQFVFSVSYTTRAPRPGEVHGQHYYFVSKEEFQKEIEKKAFLEYCEVHGNYYGTHLAQVQKVMKQGQVCVIEIDVQGAEKISKSMPNQCNYIFINAPSTEELRKRLTGRGTETEEVIEKRMKNAEKEIEKAHQLGFYNELLNDDLQKTMKKLIDLLKTFYTDLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJA8</accession>
    <name>A0A8S1LJA8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJA8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="99" mass="11593" checksum="CD24AA7E63D246C8" modified="2022-10-12" version="1">MQQQYTQGLIQIDKHLVELHNISKNDDQILESFQQTSQQLSDILKQIKTLMYVNKINADLTKIKQNNTETERIPERQTKSPAPSLQDKIQGIMNKRMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPE5</accession>
    <name>A0A8S1LPE5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prefoldin subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0360209</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPE5"/>
    <dbReference type="OMA" id="AIGRCYV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8064604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="14284" checksum="4F64E54D2077E233" modified="2022-10-12" version="1">MQQISKEKENQIQQAMDLEKDVLSTLAKVRQYEASINTLKRNIQKCNITLKELSSIDQQKTFQPIGRCFILKPKADIVNEVNENIKSHEREIDEYEKVRQHLVTKGKEKETQLQEAMKSLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQT8</accession>
    <name>A0A8S1LQT8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQT8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="42" mass="4961" checksum="90314833F3CED346" modified="2022-10-12" version="1">MKQILRDRIILRMKQWILTDSVSKTIDSLDHFYIPIPFGGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUQ7</accession>
    <name>A0A8S1LUQ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0480033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUQ7"/>
    <dbReference type="OMA" id="MDQQFES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="150" mass="18103" checksum="8DFF8ACD382B69FE" modified="2022-10-12" version="1">MDQQFESYPIQENNQDIQEMILKQLQQQAFTNYISSTQQKERNLLSENIKLKEDNYKLQEKVNMLEIKQEELVNEIQDLRQLVKRVYNEGEIHAEKLRMKNMELVQQNETLSKGLKNLQNNMECFSSIKQMSEFYENVEELDEIYQEEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYH3</accession>
    <name>A0A8S1LYH3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0460041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYH3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070876">
        <property type="term" value="C:SOSS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044818">
        <property type="term" value="P:mitotic G2/M transition checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356">
        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF0">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT B HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="138" mass="16152" checksum="9913A7E4E1FADD69" modified="2022-10-12" version="1">MMNQQMFEQLNSILFKQKFENQIKNMYPTQKNLEFKFIILKKIETQITKTYKKITKYWIADETGSAFLNLHDMDESAISPGDVCVLVGAYTNLFKGMMNIHQGKNGIFKKVSEFDLQYLTQPNHSLKNWGNDQQTTQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4J0</accession>
    <name>A0A8S1M4J0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0510142</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4J0"/>
    <dbReference type="OMA" id="FASASWC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665:SF9">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8073592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25345" checksum="73E9641DA91FBE2C" modified="2022-10-12" version="1">MEQITEEQKQAISKKNIFIFELLGTFFLVYISVCSQNEAYQVALGLFGCMLVFGRLSGGHFNPAITLAMALGGGIPYGTMFFYFLPQFLGAFGGAALSFLLLNMDKAPYIEDEPIKELAASLFGEMIGSTIFLIFALICFVKETSLSQNRIATLVILSCIYYACREYTITSSNSLLNPAAALSLTVFDCLAKNWDQMINMWIYVGGPIGAAILATMFYSQLYHSITHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5I0</accession>
    <name>A0A8S1M5I0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0450265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5I0"/>
    <dbReference type="OMA" id="QTNSYIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="167" mass="19326" checksum="37505E194698D349" modified="2022-10-12" version="1">MLYNDEIYYSSEIHEDNSSTQSAYSCCIAFLCMLCGYVLLQTNSYIEGKSNPYYYQCTMTLPLTYFCGVAFLIAAAVRLIFLVVFCLQSQKQQPHSSLFHKKVKHSEVMIAMAFFFFGSFILMVKEQCYELTVALLFVLILTAFMILQICCYDMEDDEVICEEILDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M969</accession>
    <name>A0A8S1M969_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0520229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M969"/>
    <dbReference type="OMA" id="QKYTIKC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="264" mass="31252" checksum="6C85C6087503F3B8" modified="2022-10-12" version="1">MMSNIIHEEKFTHGTEVISLYIISFDSNQNKEEFQNLIKEKQIQTIFILEDQEKILNFQNINKSLVGKDQKEQFDILFNEIYLTKKNLGLILSKTNNKSLYFFASYMILREQKNNTWLYQQNFYRTLLEQEKIQLINLIKYNPSKQNQMFLETLKIDYPNLETFSSYVINAERPGEQDQNNLNPPDDNSEQDPIFPKISIDSVIIQDSSQNNQSTQIQQIEQSNSHNSNPQFHLNPEQEQIILELAPSIKFKSLYQKYTIKCFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEQ1</accession>
    <name>A0A8S1MEQ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0510246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEQ1"/>
    <dbReference type="OMA" id="YIAQPTR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="405"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="55422" checksum="985BC8638D8CC2B0" modified="2022-10-12" version="1">MNSLQQLYLQAIIKHQEQEQVKQEEQVSKQSQQILKQQQLLSLISCSPNNLVENKAQSLIDIQNRVTQYFQQQVLRKQVVTIQNCIEYHLPYFLNVLGNLIIHGLDQIQGEAEQISILKAQYITQPIRITMSSSLFQTLSLLLRSQLVQYDKGTQYLSKFLIFRDDFCDIFFNTLYQYQITSNQQVSQQLSFSQLNSMIKKIPEDIWGTFIFKMLSQQQQKLNKDQFLVQFNQYTVNMNNFFKKSNLIFDVVENSVKQTAQMHNDSIFQEYLLIMGSFKATTQDFLKKATSVYFEEQNDEIDLNEFNIRTSRIHKQMNCSILYILPMLLQIESHMIDMIQQDKYNENAQLKNSCNYYLKMIEKLKNKCVYNGEEECNCKKCQCVRRNRNSAKESQKKKREALEKLGPLQDAYEQLQKKVKGIENENETLRQLLTEVFKHPTVSILSPEFIEPLTKVIQDTDPVQYSSSFEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MHG7</accession>
    <name>A0A8S1MHG7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0590024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MHG7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="255" mass="30172" checksum="CFCAAB95CAC86B1B" modified="2022-10-12" version="1">MGNQEGMCCLSRDKEYHVLNNELVIFREYLNVKITVKLGDISKESGESVICLYTNPEKIPLQSSHSQQVYDNLCKIGFENMYTQNLEMSNYKCITYYRVRAFESEKDLFQYYNGYKEWYYNISINSLTQLNDDQYKDILICDCPIKQSRSFSSEVLVRSVIDFIEESNNRLHIKCITIMNCDKKKSRFLKYELIKQIEESKQPALRKDRQNKFMKFITSTKSVTSEMGRQNKCDFSLKDFDLGDESKLMNLKEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJR0</accession>
    <name>A0A8S1MJR0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0600212</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJR0"/>
    <dbReference type="OMA" id="FKQMIAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051216">
        <property type="entry name" value="Teneurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219">
        <property type="entry name" value="TENEURIN AND N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219:SF69">
        <property type="entry name" value="TENEURIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035944087" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="403"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8078711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="480" mass="55702" checksum="C04B1BC860ABF6F3" modified="2022-10-12" version="1">MIFLFIVKITIAWKTQSIKENIQTCQIFESGNQQECELNDLIIFRLDSYFAICLISSEIGVQSVLYENSQGNQNFICVLKDQILIYPLPYTCGSQFKRLQHQNQFQNYSLTRDNSSMYIFGGFDGSELRNQLYKYNFEDLFNNQITYSILPNNFIQKLTVSLDLWDGSKEDFQFTSNFPQLQKLKNLGYKYTVSSILDNSQCLSSNIPKCLQYSVIVYVDECNCIKLFYGRDSNNINQNESWLYYLDDQVWQETTLDSIIKFGTTPIGQYFPNINMIGFYAENTYYFYYDWEWYKNYEQLNFGSMFKQMIAYDNKTFLLNSSSILYSDYTDQKNIKSQQYGIYICQDDYRGYDCQIQDLQCLGSICFNDLLFEKICVYCQGHGTCQNGKCICDEGYSGNDCSQYAKCLNDCSNQGSCIQYFPSPQCRCKQEDKRGGEDCSTIFCLNDCSNNGVCINGVCECINGVKGDDCSILNLKFIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKC9</accession>
    <name>A0A8S1MKC9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0640170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKC9"/>
    <dbReference type="OMA" id="QGIMTLY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="32829" checksum="1F42ED38CF37C2FD" modified="2022-10-12" version="1">MDQEDDTNDIEVQNIKEQKYQDQDSDKQKKMKVFMIIITIINIFQTLLNSIIFYYYVDKNSFFCKLSYYPDPSPNYYYYNYNYYLLGQTLVIVSLFFYPLMIILFRCFHKSTIFKKLSILIILLFFASFTLFTCGFYGIFLSFDQNSEVKSLYFYLAISDLLLAYLINSIYLCFTNYLFQRNFEIIQYVATIIITIIYMTYIIIQIKDIDGEIVTFQLIISIIYFQGIMTLYLMIQLHLLFQTLNSNYKLSLNDILYGYMQIQTRIFLFCFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKM0</accession>
    <name>A0A8S1MKM0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0650205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKM0"/>
    <dbReference type="OMA" id="NRPRICT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05467">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000541">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF106">
        <property type="entry name" value="BCDNA.GH08860"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="970" mass="114649" checksum="CF54E014050E41BC" modified="2022-10-12" version="1">MSYISFKVITETRYSQIVRICGNHQILGQWNPHDSYPLYTSSETYPEWSHESKIQIDPSFRLEFKCLIQDGDNYIWESIPNRIHKCDFRRNFLYITFNKPSMNIRTFQKYEISNEFVQEELMKVQRLNRSRDYDPFDNALDSSDNESIPELAIDERGSAKVSIRQLEADWREIIKSHYECKQKLKSISKFKKISEQYGSSDLIFDYKRKRSSSTQIFTDSYVICLSLKIPLKITLIDQFFKVTNKKDIINKYKFELTSDPYYINMYNLFSNRLSLKAIWIGWIGIQVADENEYILIQQYVYEQYKCFGIQLDDTILGCFPQLISPVFNNLTPQLRLHSEDDYMEINKIFSKYVQEALQCTLFQNQFNHLHSIFIFDYHLYLIPIYIKEGLIRKNENRPRICTIMRRSFPNPKQFRILSCSETILSSILMSDLISLIQLKDLYQFMHCLPQKYQKLTQVSGMSAFTIEVLGRKIILDYGSVGLNIKQLQNLITIDEINQEQQFILLGVDSLSLLSGLQQKFKIIEQMYLIKQYKIQLIQILYTNNDEDYQSNCQISKYLDEIQNLAKQINQNCQYELIQIRLDLSQSELVSLYGKADVFLKTSLRESICSNYLEYIYVRQYKKKAAKLVLSQWCSLKVDHRKINPNNASESANTILDYLIDQKPTFINVPSSDDWLHRLNDHLSYCEDFWLDKQLYDSAKELTIGLKDKHFCSIETTNIVSKFANHNYLDNQRIIILAYTHRFLQKIQKLDQLVQAFERLAQDENNTLIIVSDQDCEIMELNFGSINNLYMIAQDGLFIKQNNQTTFQQIVERDDQIQAKLQQLSLLEHLNLTEKNQIYTLSFKEQIKDTNAAANVFLSNLIKELRLEFDDYLIFQENYAIKIKHQYQQIEELLKMIIINETNQKGLVSFANIISFDRGWLEKIMQIFQFANLPLNKKFHCVSISDQIEKNSNLIQSNCQIDMLWKVKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MP44</accession>
    <name>A0A8S1MP44_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0650056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MP44"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="33742" checksum="FABA85D71E910EC6" modified="2022-10-12" version="1">MIKYNSENHFKFHNVKVFDKERMILYFKRMPIEQEENDNLYNGLNQLIMKERIEQTKQKIEDGKQVTSSVKMDQGELPKNRTLIIMNFDSPFDRKFIAKVFKLCGIIRRVYVGSMKVVDNNKKRLLHVGLVVFKNEYDLTKCFDIEYFQQRINNKFKSVPITTNYEKKLLQNYEFVDEKKNQIIEEAQKEGYQIAMNKYGDFETNIEKPKFEKRNWVGRNKNKTKKNINTEEIEQVIQGLDDNLPHQKKKDILKLQKQQLQQQFQQDIENLRQKQKKVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTR3</accession>
    <name>A0A8S1MTR3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0680152</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Coated pit</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTR3"/>
    <dbReference type="OMA" id="MPICELS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09251">
        <property type="entry name" value="AP-2_Mu2_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000002">
        <property type="entry name" value="AP-2 complex subunit mu, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043512">
        <property type="entry name" value="Mu2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="8">
        <location>
            <begin position="180"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8082772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="436" mass="50354" checksum="A36EE49A00F435B8" modified="2022-10-12" version="1">MISSLSFINQKGEILIYRVYKDDISRNEITQFCAKMIATKENKEFPIINIDQTSFIHISIKDIIILATTKTDVNVAMVLEFLYQLVLIFFDFKSKICKSYFQGNLDENCIKKSFVLIYEILDEVLDYGIPQIADPNLLQKFIQEGGVQQETTINIEKLRQLTFTLTGAVSWRPIGLYYEKNELYLDIIESVNLLISAKDTVLRAEVVGSIELKSKLTGMPECQIGMNDKLLMGKQQKQTRQTIGISIDDMKFHPCIRLPKFDKDRTITFIPPDGHCQLISYRISENINIPFKVNVFYSEIIDNKLEIRLKIKSIYDNNIYGTNVLVKVPVPKNTVNAITATALGKARYEVEEQSVIWRIKKFQGDFETSLRCEINLGATNKDQVWSKPPLRMEFQIPMFTASGLRIRFLKVMEKAGYKTNKWIRYLTKGGEYLHRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWG6</accession>
    <name>A0A8S1MWG6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0650211</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="QSIRYHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050944">
        <property type="entry name" value="FAM83"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181:SF29">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="701"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="568"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="450"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8081383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1318" mass="157607" checksum="FD2B32DD93F0B365" modified="2022-10-12" version="1">MLNQFAVELLRDLNCVRGNPLYFDTSLIQLFESISQNSEMQSSPCINPNDILAVINNFGTDVHFLSDHIMKQKTNDYSKLISGYRNRLSDSTKQKFKLMFNKQINNPIIYQQHLTHCYIHLYENTNFYLLLIVLQSKYVIVDSITQKNTGIHIQGWLNYPTQLTGIQFVDGDQYYELQDSDTKQKQITIENQNIWLFDLFIDNSSTQILPLISHKFGDIPIVQFFCEQQVGEVQLKEQKSLQSPSHPILKQLHLLSSRDPLQPIKSNLQNKIEQLKGQKFTGFEQEHHPKVPHTTSKNLQLFRIEENQYEQSSAKPDDLDLYTKNLESKYLNAPKFPCLIDGLISSESQMLEKSQNPYVPLTHKEQSTLSEIRHQTVNTQDSAINEHLSNMLDQKQLQNIYQDLCREVEKPQNNLQQSQQIFQPYNVESLYFGNSQQHSNRNYESQDLFLQQQQQQQQQQQNQFYNQQQQQQQQQQQQQQQQPFNQDYSQQQQQSYQYQQQQYLQQQQQQQQFYYEQQQQQLLQQQYQQQLYQQQQLQYQMMLYHQQLQQYPNNLQIQQPEEQYYFEEPQLESPPKKWSFSPQKQDESIAIKKRTSNPEPQNKTSYRYSIRQSIKQQKKSEFQLSNTQKILQKEGAQIKDNEIPFKCKLKINSIHPYDFVDLRSNRAQKKMKEIEQGLLSQSIRYHKDHNFNALYRNEKFSDIVLIVNDHQYKSHKSVLIQVSNYFKELLETKQFELNLQVDNLQLFDMVYKYIYLGKLTDSEWNEVGIHQSIKFYRLAEKLELYQLIEQLISRVVIPMMINEFVLDWYIIGFRNQNSHLSYIFKFVYVYTVTYFSLNCKTIIDLIRGRQDDSIIKDQQMQNKYKKIFDLMTPEEIYFLVQQPLMQTDFEGVDNLLWLITEKKQCQQIDLLKQLSMDHLSNCGFKYKDFTNYQFEQNLPLLNNQIIIYPQRLQETKKRRSSIYTDQEFHYSESLFDEYSFYQQNSNTCKINKLVYEMKISNVVGNSIVISECFASQSLAWRLIIDINDKNIISIYLQERGSIKNNLKYMNLEDNLMDESLPQYTKLNFISVLVKISILNKQEQSNERVMYHTYPTNQFHTVGFEDIQTVGSSLWLKVYIQEDPLHSALMHWCGYNFNDKFCQLPYFTLYSLLKSDMLRISEEKQILGLLYNLQKSEQEQNLDLVIHPIRYFYVPIGEIFTLSKDCQTVRKNKLFQSILNLLLSHYLDGKQHNQKPRKTYAKIDILINQSDFKKEWLKWLIYSDNKTLKINTNDIKKEVIEKQKKIERQQYQQYFQVYNYTPKKQLEQQQQQSPKCNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWM3</accession>
    <name>A0A8S1MWM3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0720186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWM3"/>
    <dbReference type="OMA" id="SHYEYMR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1083"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="160"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="382"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="775"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="856"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="332"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1094"/>
            <end position="1115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8084640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1115" mass="134331" checksum="50BF1809C28BC475" modified="2022-10-12" version="1">MNNKWPTSNQAPLQQSRQKQEIDNSNVPNNQPAHHVKNDEQVRQLCQKDSPISSENRSRIKSSYDIAKSSISQKNEKTETSYSPIIKSTYQRDISPQFQKNENHKLLDSYEVRLMKLSQENGQLQSQLFSDQQKYNQIIIDFELQTKCLEQKLQTEIMNIQQHKLLIQNKNEEINASTQELLRLEQKYFDLESKMFQQQKDMGILKQSNDSKTIEIDNLRQYIKELQAKNVRDQEELKLATDSSIRINYELQIKEIQVQHGNNIARLNDLLQQEQQKYNRLLNQFEQQMNELNYQNIKLNDLKIEKDKLFDENAKLQRFLEESKVQQLEFLRESDSQRDRQMQQDKNHQNTLNELKSHYEYMKKSQVEREVKEVTIKFQADKLNYESQIRSLNQRLIEFEGKINLKNEENRQLQQKQQAIESELRIFIQDHEHYKRIKESELQEYQNEFQKFKQLQFETQQKNQNQQNEIQRLNQIINDFKTEIDLVRSQSQEIQKQFLIEYEDDLKNQKLKQQYEIKQLQDLINELKNQNQQQELIMKQKDEIVHQQKLNVSQKQGEIDGLTLKLENLEKLKVQEINQYQSEIENLQREQHISLNKLTNEKSLLEQQIKQFKQKLNDFETQQIQQEFNKEQERQELYQKLQQKEFQLQQLQNEKNSISSQLTVYKQKIEQFDQIISELREQNQQINQEIEDQKQQNESDRAQFVKKELGLENKIQQLKQQINQREQELQQYLSELDTTNNRVRQQELITQQSQDEFRRREHQYVQEITNLKQLMDTTQQRQSEYQSMRETLQEQVQSLQEKSQNKEKEVEDQQQKYEDLREQFNQYVEETNTKLEYYQTIEYERKESDLKYQAELNAAQDKIRVLEIELETLQLRQNQLLKERRELEDLLDEKSKEIESLKNQLNKDYISNEEYTLLQKKYNEQTDELHLLEQKQIKFGTEKKQLERQIDKLNQDIELKDQELLQTDNLMKKRRSEQDELNKKIDQLTKDYSKVSHDLLDVQAQLSTKNDQINALKRENEDLQIQLASAKKQYEKTQEQLNKKNQELLEKFNDAEIKKSYSSGENTATKVLTATTIIKTSQVQLNTPLIPEEESQKGTERSDQQFKASEKSQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWM7</accession>
    <name>A0A8S1MWM7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0700198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWM7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="30640" checksum="D6650DED701DF35F" modified="2022-10-12" version="1">MFKGEYQNGQLFILGLNLMQKQYQVNVINVDLNYIALIFVNSKIMKEFLKNANPTIRLSCNQFDNNHPFKINAQRKEKIDYEIFCVICIKGYKLMDQRFIKTCPNLFLECNILMASINVKDVKWIQNLENLLYVIINIGFYHNHQSIYVQNNNTIYTVGSPEQGLFIDYFLQFNCIGFILSHKFIFEIQYERMIWQRIFSFFIYLFQYLIFIELKLLYHILFALLLFYSQIQVFNFQSKCHQNNSHNYVSIYSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXQ2</accession>
    <name>A0A8S1MXQ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">V-ATPase proteolipid subunit C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0670148</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXQ2"/>
    <dbReference type="OMA" id="MVNVACG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033177">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, proton-transporting domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18177">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6F_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18178">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6F_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000002">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="10">
        <location>
            <begin position="34"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="10">
        <location>
            <begin position="124"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8082226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="194" mass="20834" checksum="894A354BE18F1214" modified="2022-10-12" version="1">MNWGNPYNWDAADWQQATWGERFMFIDPYFWSYFGVGLALATSIIGASWGIFVTGVTLLGSTVKAPRIRSKNLISVIFCEAVAIYGVIMAIIMIGKVQTIESYPDGQEDECYTTALFNGYSLFWTGVSVGLSNLICGIAVGVTGSGCAISDAQTPETFVKILVVEIFGSALGLFGVIVGIIQCSGATFLKNCTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MY82</accession>
    <name>A0A8S1MY82_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0690096</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MY82"/>
    <dbReference type="OMA" id="TEYYCEE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035895412" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="14629" checksum="22CB893373056AC1" modified="2022-10-12" version="1">MKPLLLFSFLVAVRSYCYFIQYGTEYYCEECCFWYEGDWHCETYQYCYTFLGIIIAIWCAIIGVYAGLYFYAKNKTAKRLRVIYQQSQNCLRNNTQNQIPENNEQPTQIAQINFVELKEQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZK1</accession>
    <name>A0A8S1MZK1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0630220</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZK1"/>
    <dbReference type="OMA" id="FMESHYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051889">
        <property type="entry name" value="CEP41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44390">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 41 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44390:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 41 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="128"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="233"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="245"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8080354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="290" mass="33745" checksum="50AD4B1356B8C0AA" modified="2022-10-12" version="1">MDKLPIGYVKGKGNPLDKKVPKNKQYDHIKQTLNTGPTVRDIEVVSNAKIAKKRSELFKRIKCSTVFNYINENTEQETIYKLADQQQQEQEQLQQFDTQSQYSQKTRFTEVSQVSAITYATEQLGITDQSEFLLLDLRDPDEFELYHIKEAINYPAPNLRQDKLTQQIHRFKNQKDKHIIIYHFDEKNGIPSATLFAEKGFENVYLLSGGIEKFLQNYPQCVIGIKVPSIPKPEENPNKIIKRSNYKETDSQINKSEKNISDSKSQISQKTKFTRQSSQQKQQQSQYIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N188</accession>
    <name>A0A8S1N188_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-related protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N188"/>
    <dbReference type="OMA" id="PNTLFES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="674" mass="78319" checksum="468AD9BD009DD9D9" modified="2022-10-12" version="1">MILLIIFGTVFGLEYQKIFSYNGQYAHVISSECNAKSVEDAPRLYNIIEQQYQFQWLMTCSDDSECWAKVTFSDEGTNICCYKPNQQQDNVQNTIKSLQDKIQNIENLIERGTDCIIIQYGSHSIKMNKASEMLPKKFRTLIGYKVQSQLKQVQSTDLTVIDESLLLLEQKLISKGALKAQPKSMRGKPRAKIEVDLRNIRGFLGKKEQKQPQCTLTQMMEDKSIVFEDDIFTGDYLVRQPIKHGFFNLTENYTIQDVIEDLYKLTLYGLNCKLGLKNISDYYCILVIPDIFQRIQIKMLVDMLIRQLGFKGIYLHLESVLSSFGANLQQCCVVDIGYEKINITCVDDGVILPGTYVRKNFGSKDIDLVLMRQVTKRNACDQTVQLQSNNYGDLLQMEKLKEKACQLIQKEDKLNYNYELHCLRNQKEKRFFVQHNDGLYISANTLFESEPFNEIRKQDMHECFDFTGRLFEMQYDPEDNFEELSGGIQLSWYWGSKEKEQQYFIEGNQMLNPNYMIPLDHMISYSIAQVQDPEIRQKLANNILFTGGGAHLIDLVDEVEALLIEKFQVMEVNEIERVEVKTIIRDIRPINMAWVGATVLPKTESVSELWITAPRWLGNLEPQKDELEDMINQFDGDVQQLEKKLIAFAKKDKEKDRSCEYGIKHLKEKIPFIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N3D4</accession>
    <name>A0A8S1N3D4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0710233</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N3D4"/>
    <dbReference type="OMA" id="YLQMMEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8084213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="283" mass="33826" checksum="3E9174FCBE6AA60D" modified="2022-10-12" version="1">MNKFELALQTQQELLNYILSTQWKDDLEKQQLEVIFQKVQTTCNIKLEIDEFFTKEKQEQLKSYIYEKQQQIRKINKNLQETLNKQAQVRQSPTCVIAQLPPRFKNNKESYQQQINYFKNNLIELKNCHQKLCLDIDIKQQSETIKKQINLLQIAENFKFELNCLESELMQNRLYLCEQFDSIRKQIEIIIDDNLLQNLISYCSAISKGNILLKKQLIIIKQIYSFDTKDFYLQMMEMDKENKQTSYSQDTDVTSQQSPQKNCDQNQIINKNLRNQECLCVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5D1</accession>
    <name>A0A8S1N5D1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0670157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="MCINTIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2358" mass="273108" checksum="5213317F2EBB3497" modified="2022-10-12" version="1">MLFIFLIVTQVYCQDICRQLNTYETCIGSKTEYCQWYPHQRYCRKSDDLMQGCDQNLSVKLCTRQISTQMATQALCIFDSICHKINDITKAKCNQNLNKYGCIGITNPQQLCKWEDKQCQYLSDEEIANLNTNFPNLILSMSVCPLITGHLIAHSNVLDSLSSSDITDYGLLAQQDDMLDEQKSYDEGNLQNNYLNSQGQFIWYVPQKQSQFNLLNLQINDQSRVGCIGIDISDDQMFRTLFNIDDQKVINGVNQIYCQYININPFNDKMSVFVNNKCEIINFEEVEQRTDLKCENLGRYACQTSPKNMDCQVVQPINQYEKSCIQVQTQSVNVDCEPLNGIATHKQCSQSSKYCFLYFNGKEGYCDGGCYNLITKNDCFKKSNCYWIDEEQNFLVQCSPLQGCTQMGLSRIYCENLALPCIWMDNQCKNGDLRILTCSEANTKYSCTHVQRQDQLCIWYNNMCINTITHLIFKNYSQLPENLIRNRNQCLMQDKGSYRYDEITKQCYRDYILDTQDLSNINKYFCLSLQLNSQWNYEQQKCQIIQPLEYCDNRMNINPKLCSYSSNCIYDEIKQSCTQLTGSISCNTIGLTKTLCISNLNEFCAWINNSCQTIKIFDNCVQLVNVSPYSCSMIDIEPCSYHNGYCIATINNNNQCQQFMNKVQCQKQQSECYYDLMDCKQIDNPTQYSTLNCNGLTQTVCIKNQKQPCIWYENQCQLYKLQYFDVCNNSVNEKACQQKIHNGKLSIQHFCEYDTVQQKCLQNINNIVDCGSNLKINLHRCVSFTQSDCYFYNYQCKQLSTDSNYKDLQLSTLKCSQVNTNICQKIKTPDQICIILNQGTFSTCIDISLTQQYANLYTCLQINSNLNGSPSICSLATDNCYYDSGDNECKIQTDTNIIYKCDDLISKSLCLTQTKYQCIFTKNRCKYYNNSYNNIDCQFRNIYSCETSGKNCRWNSTDKICEDSTNYCPVAYNQSHKVCQNGRGYCFNGVNGCVLQQLYKELPCNIALSQQLCVSQNHLCLWINNKCSFYPSHIKYCYQLQTQQECLSIQHLSCVFYKSQCIEDSTLNQISDYYDAESYQYCFNMQMNLYSNSQQCIQVLGNLEHQQNDIENTFNMQCQQTSSMITCIHQRSSKCTFNQQKCITSTLTSCIQEEGIFHSPQTCLSIPNCFWYSNKVGQGFCYQNQLSCDNIQLKSLCLSDFGLNCKYESNKCQDAIFTDCDQIQNIQVNYKVCKQLQPNCFYDFENNLCKKVNRKIIECTDAQNFYDCVYAYKKNCLVQYDIDNKYEQCQSISPNSFNANNNLNSCMELTVNPYKYNLKTYTCVIVNNEQEIEGCSNINQISCLKLTKQFQCIWYNSICQQYQLQDDLECDQLNYQVCLIQTLKQCIWLNDQCKEFENQSDPGILYSQGYCQGKSEYWNSLSNSCFTKNSDLVIIQCDNFGLDKSSCLKQNYIECYWNLQKCTSIINKVNLTCDNISNYNCDTDFNCKWNTGVNKCQDNSNSNLECSDYTYHSCIEISNKNCLFNYSQNKCQEIQKNSIILQNCHEYLSFQLCRLVKNQICTIYDKVCQEWTPRLFDCMYIQNQYGCMYAPMACQWLNNQCQYIDLENKVLCKDLPKSYNKRSCQQQSIDDCEFDDILLQCSQKIIRMEDINNVHLGNMLDTNLYLCEDQLNYNDCMSNFKQQCQWLNDKCVQSDLNQCMNQSYLSCLNNKSNCKWRNKRCYQFDNNENIEEIPILISPTVCSIFISDKKLKYSETVFSCVLSDSEIDDCDTIGLNDNACYNIKKSKCKFTNNICSFYQLDVQYQNCSQYKNINSKVCSSLQISCKYDESLYQCVSATDTDFCTTKGLSKKACLSITTEPCYWNNDVCSLFIADQNINQCDNYTQTNSLACQYIDYQQQICTYDIINHVCTNIYNRFQKCTQPGLNKYACVGLLYEPCQFVNNQCTPFTSRTSICYGIENVNSLACAIQVLDKCNYQKAIFSCYGVPLKIPCNSLGINQVACGLQANCVWDHDTTSCLYKTTPNINMCFSLIEDSCIQNRSCYYENNKCRLKRCLDLNEEECVNIGSLNNEICFLDIFNKCQIAKECEDIVLLNEKLKCDTIYFNQSSCYQINNNCVSSQALDKICPQTDCSESFCVKDNHICRSPICSDFDSNNCPKNNCAFMNGQCTQITTCSDIQELQICNLSTINNQQCSWQPSALRVGPYHCTNKPCYLFGSSWQFCQGNEMNDQTCFVTKYAQCESCEEQTDQATCLESKLCTFTNNKCKSILCKYFTNEQMCVAAQRCYWSQIDQVCRKNCEKIVEQEQCDILDYECTWNRFTYICENGPQQNPDLSYFINESDTSANIIKLVLIICLFYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5K3</accession>
    <name>A0A8S1N5K3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0730103</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8085016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5K3"/>
    <dbReference type="OMA" id="WVIAEYN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057496">
        <property type="entry name" value="FAN-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF123">
        <property type="entry name" value="PROTEIN FAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25400">
        <property type="entry name" value="PH_FAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="3">
        <location>
            <begin position="226"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="2">
        <location>
            <begin position="328"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="620"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8085016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="733" mass="85810" checksum="4E1713CD521111AD" modified="2022-10-12" version="1">MFPSINSIKKPKKNFNLLHLETQEQYFLSLKVALIPSAKSDEIRGKLHLCSRSLVFEPKSSQLPLMKMKYSNGLFMKILKNFEPKSLGLLLSIRWGHQLMQEDIDQLDLASILNQQKGEQKSNELLEKLYNKQRKNMIQLVCNCFFIKVDKLFIVNRNPISPYITETCDDNILFTISKSNSKQILIRFLKLYQGINQAEDEFTFINQTIQQTLNDSLLEHKQNVGSEGHKIEFVHKVKLIKPEGIIFGLFSIQRDEILQFIPLINSPQGKIMQYMFTDIKYFLKYRYMFKNNGIEFWMYNKKRSYLFVFDDQTTQESVYKYFQKKASKHCLSALTKEKITEEWINGNMSNFDYLIALNTLANRSFNDLSAYPVFPWVIAEYNEKDFNVNSPSFFRDLSKPMGALNKHRLQKYKAFYKDQLKDKKQDPKPYLYPTHYSSPGTVVYYMIRKIPEFVIKLQNGVFGPTDRIFRGIDSTWFTTLNLHADSKELIPEFYGLDSDFLVNCDKLELGITQDGEVIDDAVIPAWADNAPDFLAKMRIALESPYVQTNLPLWIDLIFGNKASGNEAVLNDNLFYPYTYVENVNWDKCKTDIERQALEIQVTEFGQVPVQLFQTAHPQRKLKIQGNLKQLEDEKKELKQQNLNLQNKIQKLTQQIQQSQDKELVLQLQTQLAALQWENEKLKFNLETFKQEMITNKEVIQTFNSQFDQEEFDKNGFDFLNDDSLLDSSKKPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6C1</accession>
    <name>A0A8S1N6C1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0820124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6C1"/>
    <dbReference type="OMA" id="ATQMKLC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="363" mass="43295" checksum="D57035D1BB8EBAE1" modified="2022-10-12" version="1">MSTQKSEKSIKNHQQDEKLKKLFMKITLNSLRKYFNTKQLPYHPLITCVSIKPGCINRCASLQSYPNQSVWKSLLELPNEMQQEIVEFLINELNAKQQKFSSKFLIEGQERIRNNFDFQSFYKEPIKPDVLPNDNSSDSKKIEDIEIKDDGTKIKQKKWFKGEDFVSESKQIATQMRLCNQIPPLVTVNSIHYHKMRSLLEKIKEQKFKQSEEMKIFVEELEKFIQKDPKSVELKKKKKIKKSNDKKDHQHDDQESIQRSKSLQDEVKVEDNSAQDFLDQFKMKIKQIEPGVVLYLPQYKKIDPQYHQYYKNLEDSYFKDLYYKVKVDEIVLNTKNILRFARQVFQNLDFIIPYKQENLNSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCQ3</accession>
    <name>A0A8S1NCQ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Isopropylmalate dehydrogenase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0850008</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8089922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCQ3"/>
    <dbReference type="OMA" id="WVFFREN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000014">
        <property type="entry name" value="Isocitrate dehydrogenase (NAD(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="9">
        <location>
            <begin position="20"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8089922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="348" mass="38369" checksum="AB25899A0F0F4B83" modified="2022-10-12" version="1">MNIIRKASTTILRPQFNYQKVILFPGDGIGPEISKAVIDIFDSAKVPIEWEFHEIHKKRVTEQGDLITEETLKQVKALKYALKGPFETPIGKGYRSINVTLRKRLQLFANVRPCKSIKGVKTPYPGVDVVTIRENTEGEYSGLEHEVVPGVVENLKIVSYNACQNIAQYAFEYARANNRKQVVACHKAGVMKQGDGLFLKVCDDVAKNYPEIEFSEEQIDTMAFKLANDPTKIDVMVMPNLYGDIVSDLCAGLIGGLGLTASGNIGKDCEVYEAVHGTAPDIAGKNLANPTALLLSGIMMLKAMRLNDYANRIENATYQVLEEGKFLTGDLGGKSTTTDYTKAIIDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NE13</accession>
    <name>A0A8S1NE13_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0800127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NE13"/>
    <dbReference type="OMA" id="ETVFEYH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051218">
        <property type="entry name" value="Sec_MonoDiacylglyc_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856:SF25">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035884903" description="Fungal lipase-type domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="81"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="271" mass="30615" checksum="28F509A887E16638" modified="2022-10-12" version="1">MKIVFALIAIVSAFNYDPSLANELTALSFAAYCHPENILSWNVGTISQQYPHLSKIQIFENIELETRGYIAYNSHSQAITVVFRGSSNIKNFIADIDAKKIEFNPICKCQVHEGFFAAYTSLKIHLDVLLGEYRIKYPYAKYHVTGHSLGGAMATLFASELSMMGIKVTLVTVGSPRVGDSDFYDWFSTLKVTHSRLTNKKDIAPHLPPVRYEFEHVNTEIWYKDGVNYIICQEIKGEDQKCSASVYSPNLTDHLTYLGWSSNSCKEQLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEH3</accession>
    <name>A0A8S1NEH3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0780233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEH3"/>
    <dbReference type="OMA" id="ERAIKWM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF661">
        <property type="entry name" value="GH16847P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="37731" checksum="BDC2A61DFBEAFD4A" modified="2022-10-12" version="1">MNIYSERAIKWMKVLGYLPSDGINQFLGEMEHKYNQYIMDITKSDEMLIFEQLSKYTLDNQAQTKQQFEEQKIKTVSSYMNIEQYQKNEQVYRMIEIDNNRILSKCEFLNNSCNYKIEQLFYTDNDFYKQNYNGVQNSTKLDLLKRILYIYSLLHPNIGYISGMNYILAPIIKVIQKEADCYFCFELVMEKQKHLFLQPDNEKGLQAIMNRFDELINNQEPQIFKHINQLGFVPSHFFVGLFITLFTSYLDIEIVIELWDNMLAQEYQDYQFKIILEMLRVLRDKILKSNINQFLELITLKNENQVNIIKSILNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NET4</accession>
    <name>A0A8S1NET4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0870135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NET4"/>
    <dbReference type="OMA" id="NFKMAFD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8091110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="62553" checksum="0963308B3D22ADD2" modified="2022-10-12" version="1">MNTKLETIPVLQEKCKFHQNEQANLICLTASCLNQGFFCFQCKNHHEGHNVIQGIEFFDSLKQLVQYDGSQKSSNNVNQVSARIVTAMLTTIDELEALNQYFSETLGQIRLQFEKYCQISNDVNVINNFLIFSQKETIEPQQFKDKFEECLKRLCLKLQKLHIKSTVTCQSNSLFLPDERLFQEYLEPLISTLKGIKLQAESSFTKPLYYNKLDLNFKNGLMPIVNSFFEPVIFETDQPKEEDKVLERLWIDKNETMRKMLMMKQRTKEEQVIEDEPYSYGNGRLEKVFAVAGSKVWFLDFIYSKFKPTSKHSKIEPKQACIICKCHQKFARINEFLNCKYGDLYGPYHLEARLLDNFKMAFDKRTNKEYLIDRGGLYFHDLCVYWSSLVEYDEKKGTIDFESLQEAVKISQETYCYLCKRKGPTLKCNNENCQIWIHYYCWKELEPSQQYLDNQKFRMLCYQHVPAKYRKPLWASQNLLLDDQQLEKKEFKEFRQSQFVKFTYQEAGQSRYIQKLEQQATKQQFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGN9</accession>
    <name>A0A8S1NGN9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0800124</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGN9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="2">
        <location>
            <begin position="212"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="75"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="545" mass="64660" checksum="6E387811FBF4A6EB" modified="2022-10-12" version="1">MDYLPNCQIHDNPIIFLDLQPKISKGKRALCRLCQLNRPTPIEKALDIKKKQIKSELEQYNEYFSSFLYSIERTIKDLDTVKSQFSKNIQQIEENLIKEKEKLLQEKNEIIQNLNLDFQCLTITQLQNIGEELSQKNPQNSQNFEKNKEKFDQEINQIMKCKELKILSDLITLQDKLTLILNQKESEPNQNKGFIEYSLNKEELYLNQKNHVLLIGGSGSGKTTLFNKITNSNLNVEQGQQITSDIYIKNSQYGEGFTLFDTPGYVNQGMYLKTLPAYLYTLENFEISRIVVVIRFNRLYNTIDSYGDILTVFKRYKDKITIVITFFDQCIKKEYVQNQIISQFQQHQIKSIIFTQIQDTGETLCKLLDTEIKSSYQQKIQLKETEIIQILKLEEDKNCQCDIKDKEYNLNCKLKKILKLPEMNNIDNIQQFQSSLESIILQVIKTNFEMFQRIEKKYYNQFQEFLYLQSFINFTNGQITQILIVAEQKMNKIQWDQYKIKLQKYIQNQMISYDNSLEIAHQKCINMFKSYVKNVKFYYYWDISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGV3</accession>
    <name>A0A8S1NGV3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0750139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGV3"/>
    <dbReference type="OMA" id="KAESQFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="261"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="365" mass="42969" checksum="5D14F8A358B2999C" modified="2022-10-12" version="1">MNVCSNFTLSLTGGFQECRNCGREKKFHRLDFENNNIEYVQKASNNNAAKFQQEKLQQIAAQKQVEEDDFRAKACNNFVLALIGQFQQCGNCKIEKKYHKLDTSGQIAYQSKSQVVQPAQQTQQQKKTTAIAEVVQETMMVSSVKDRIAQMNKKVSSTNEPQFRPSVKREEVQEKIPGQAEIVTEQVFTSVSDKKSVFDKQRVRKTIEQSTQQIREQQNSEGIRKAESQFITSSENTQEYQEQQIQNQNEQQENHQEEPAQDQQNEYQEQQQQVEDDQAKEQQNEQEQQQQQEQQEQQQQEQEQYQQQEQYQQEQQQEQQVIPQQENQEESNNQNEQVEESQQQNDQHLDEQQDIQQQQEANDVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHR0</accession>
    <name>A0A8S1NHR0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0820182</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8089003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NHR0"/>
    <dbReference type="OMA" id="NDSENCT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF98">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="165"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="YTH" evidence="11">
        <location>
            <begin position="454"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="612"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="612"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="638"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="687"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="702"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="779"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="789"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1033"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1087"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8089003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1265" mass="155145" checksum="7815ABDD28CF2622" modified="2022-10-12" version="1">MNYVRNGQLCPILLKGVLNRKQEPLFEVESLEQRIKRRLLNVIYLSKVMFHVTILVWECDRRKQNFLKNLNIDQIYLNKNEEEDANVFIFEEDQQNNIIQKQTPQQILFKLFYTLGFNLKNEIFQLEDPTQDIIKELYQLIFEDLRNDSQIRISDKMIITHLYNLKILDTKYNKSHSRVYLIEKPYFINSERNTIVVKWVKYDSQENLKLYLHREIKLLEMVNDSENCTKMFCYDNIYDEQLIFMRFYHATLEDLNNKIGKVQLDKIQLLYLIKQMLLALKGLHSRQIIHRDLKPQNIMFEYLNENQNILEMRCVLIDFDRSDQVQRTIKSNLNLQSYYEGTPDYQPPEGTQKQYTASYDIWQLGYIIQCILLRDKNKNYSRLKDRPDQAQYLNIFKSDHQKVMEQYPEFYNLLMKMMDYDPTKRPKLEEIEQIYIQFLLLILIMEEIFKFPQSKFFIVKYKLKAQVDSSLNNKVIFIPKKFDKQIDRVMEKSKYVGIFLSCSDEKSFLAFGILQSQSIEFEFLTEDQKINLSDLKGNQDSFFKVDWTLKGVLSYEKTQKIKNDLNNSLPVNQSREVQELSGSNAEELHSLLLKQKTYKPQPLQDIRLPKSYNQQTLPKYPNNSDEQISRKKVQSRSRSRDKDKEKSHDKRKSSKDTNDDRGKLQDKRKHEQHERKDSYRNSQDRHNKSRRSRDRRSREKSKRSQDIKSEDRRSKDRKNRNDQNKWQFHKEFVDDKQVQSEVGFKDKDRLEKEKQQREQRERERAKGQREQRDRELQKMTDNINLRQSSDWDKDKRIEYEQEQIKLKDKEYDRLKENDKAKEREMEREREREKEKQREKEKEREKEREREREREKEREREREREREKEREREKEKEREKEREKEKEREKEKEREREKEKQREKEREREREREKEKEREREREREKEREREREREREREREREREREKEREKEKEREKEKEREREKQREKELEREKDRQRQKEKDRYREKDEDKQYDKEKYRSNRYEKKGEHYQQKDKNVHHYDDKKRRRSRDRSRDKQNYSNDRRDREKRVEKDRSDRKHKIIKQEVKRKSSSSSQSQKNRQVEIKITQNPNSKNIKLQEDLKPKRKYEYENEFYSKSVSLSPHEKQIEIIEPVKTPEKIQILESIPVQNTLPQQNQIPSVNIQPPKQPTIQQVEVVIEKKEETPRIVIQENVKRVLVLGQEKPQPTQESNFQLQTGSMKIIKSATGNTTQTIIVQKQTTQDTKLGQNHLSGQEKIKDRIIIKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJA4</accession>
    <name>A0A8S1NJA4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0880063</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a fundamental role in microtubule organizing center structure and function. Component of the infraciliary lattice (ICL) and the ciliary basal bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the centrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJA4"/>
    <dbReference type="OMA" id="EFMRMIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF59">
        <property type="entry name" value="EF-HAND SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="105"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8091429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="174" mass="19792" checksum="D16F536646D54C9A" modified="2022-10-12" version="1">MNKKQQAPVAQKPVGKQQQVNRKPQDRPGLTEDEIEEIKEAFNLFDTEGTGRVDPRELKAAMQSLGFDQKNPTIFNMIAELENEGTDIDFDQFLDAITSKLGNRETRDGINKIFDLFDDDGSNSINLNNLKRVSKELGETMTAEELAEMLERAASNGRDISREDFYNIMVKRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NL88</accession>
    <name>A0A8S1NL88_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MutS-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0850159</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NL88"/>
    <dbReference type="OMA" id="YKFEMEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051026">
        <property type="term" value="P:chiasma assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF20">
        <property type="entry name" value="MUTS PROTEIN HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein MutS core" evidence="4">
        <location>
            <begin position="325"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="5">
        <location>
            <begin position="668"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8090223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="870" mass="101862" checksum="E7197FB2A07B8DBB" modified="2022-10-12" version="1">MKPIHTPINLSLSQLYQSEIKQQQQPDRILKDLNGSAFKFKFDSNFSDRKQRQPQQQQQQQYQTNLDCGLRKSSIFEDYKFEMENGNSCRNNNQIYKFNHLNSSSEFNYQKPNIQNVQGQYQSNQALIGSKMLSPLQKMNHFIQEDLESLRFSQQFQKLHFNDNNVDDYKISLYYYKPQIGITYQMKDNLLLVTIDQSQITKIRQYLNEFQIDTLIIFYSINFPQQLLMSIKQIIQGVVKFCNFMQWEEYNIQDKLTQVGFMHPEIYNETLIDKQIYINLCSALPILSRSNLDTIINQLQENEKCCKLKFNDSLQNQLGILKQENKQLSIQQLFEKYFQTFHAKKIFLDYLKNPISSYQIINKRLQNLYIFQQLSLQPSNQKFIKMPGKIGSFTQILKVLSTYQEKSEPYSKLFTQLQYLIEFVTVVKQLVVIDRRAQQQTCQLLKDLSNVNLESICQLSDIIETSLLFNQSNVQIKEQKYPQLEQLLSLQINLKTRIQLIKNKHQSELALVGIKPQSIEVKYVNNSYKLVVQSNLNLEDKVQTLGWEMISERDAENFVVFKTSYTLSLDDQFGDLENQIISMQEKILIELVKQIQSYSDQLQQIEQLIGEIDYYIGLSIAITQMRLCIPKLNNNNQMKIQGGRHLLVEQTKKQNVEFQPNDFVLNDKRIFLIVGPNFSGKSVFIRQIGIIIYLVHCGLPVPALNVDTYLLEEIHALFPCEGKQQSNLSITNLELLQLSSALKVSSRSLVLMDEIGKTFKYQVGSNLHKSALEYFMQMSPTPPIVLSATHYQNHEELLQQKCLQQGEMEVAFESNKNLIFVYRIDFHSKRVQKVSFGLEVALLVHQNEELYQRGLQLYELLTKYKYLQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTK0</accession>
    <name>A0A8S1NTK0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1000020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8095958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTK0"/>
    <dbReference type="OMA" id="HTSYIFC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="264"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="56359" checksum="63EC68700EDA51BF" modified="2022-10-12" version="1">MTQDYYKQKIEQLTETLLDQFESSFNKFENYLDSLLNLTDSINSNYQQIAFPSVNVPQTNFENLNFAQQLFKEIQPLIKQNVNNQVKHKKKPIYQEQNLQMLGVKQNMTSEFQKLKIEKIQNQMEQKVSDRDVLLQESNKNKSLLCYQSNNEQFETVYTNNQSNVKSFTYQLVSQYSISQNECCRAIAINKDCSIVLAGCGKEIKVYEFKQGFIKQIQILNEHNNDVHTLNFMKKSNQFISGSYQSIIIWQYNQNNLWVTQQILNGHNSWIYCLILNNNEDLIISGGSDNTIKFWTKNNEWTCQQTIKDHFNSVFGLSLNQQQNKVVSCGNDKQILIIEQQRQNKEWIVIQKITVELSTLRVRFIENDMFILSQCGQDQISIFEMNSINKQFTKTKDIVVKCGSDGYCLFPSQYINSKSILLSKNGEYVNLIKKKQNGQYLTEQSIHFGTNVLFGGISDDGEYLITWENKSKEIQLRRYNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYT0</accession>
    <name>A0A8S1NYT0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0970110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYT0"/>
    <dbReference type="OMA" id="AFRFIFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="304" mass="36359" checksum="EF7B3A60C92C0834" modified="2022-10-12" version="1">MSQLFTNPLIFTNLINQPQLFTQHFPFQNPLQTYYSQIQVPSNFAPFQNYQSQQYLQLQLPFNGYHDYVIHNENSTNSQKTSLAIGNSPTTYQILNPIQQHLLNKQKLREYIVLIIDDYHQIHDITQNLRNINQTQLAKILEILATKQQQQIKSREELIKFCLRKAFRFIFKKISERDNISKTNLKTARQEFLTIMEQEKKIPLILPFRKNSKNKTMNNDFLKEVFSSQIFQSFYKEFLDHIDDTIQTDRKKKIDKLQDKIWLSLRDNRTTTFDIKRLPWSQKNTERVKSIAFELLTFSQNDQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P104</accession>
    <name>A0A8S1P104_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1000130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P104"/>
    <dbReference type="OMA" id="YMQFREF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="137" mass="16378" checksum="46E0E2263F74FE15" modified="2022-10-12" version="1">MIFSFFYSSKNRLDRKRLPLQQTRSENQSPSNYPHIYQTQPRKQQICVQIQKNDKSDETDEEDIKSSRDCFLQIGTSEASKLINHKNRIKLRSVSMQKKQIQYMQFREFDNLDQIAEEQLQLQFQNLAVHVGQFQHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P3U7</accession>
    <name>A0A8S1P3U7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1050008</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="QSNVGHT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="213"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="255"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="297"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="339"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="381"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="390"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="423"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="465"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="507"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="971"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="991"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1013"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1055"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1097"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1139"/>
            <end position="1179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8097732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1247" mass="139420" checksum="24A86BB0871E8FFD" modified="2022-10-12" version="1">MQGPFQEKEVLADIFGHIKDVDELKFGVILEKLRKEKVQDIIGYLSDNKNQSQLELCILQKGVDITQAEVVKNDIKQITEVLRKLKDHDFNKIDYSSEKNKESTLGLIKSIKDNKRIIEFLQFLVHLTSIDDTLTQCGSNSLQILVQMQVDLRNKNLENIRIQNTSLVGANFVRCNFSGSEFNNVNISGINLNQAQLFNCKWKDLRIHELNQLGGHTNHVTSVCFSPDGNTLASGSYDNSIRLWDLKQGKLKAKLDGHSDHIMQVCFSPSGNMLASGSRDKSIHLWDVKTKKKIAKFDGHTQCITSICFSPDGNTLASGSYDKSIRLWDIKTKQQKDKFVDHSDYVTSVCFSPDGITLASSSYDKSINLWNVQTAEQKVKLIGHSDYVRSICFSPDGNTLASGSYDKSIRLWDVNTGQQKAKLDGHTREIMSVCFSLNGNTLASGSNDNSICLWDIKTGQQNTRLDGHSDYVTSVCFSPDGNTLASGSYDKFIHLWDIKTGQQKDELDGHSDYVTSVCFSPDGNTLASGSYDKSIRLWDAKTGQQKDKLFGQFVSLLMEIHQHLVVVITLSVYGIQKRDNKILDWMVIAIMSHQSVSLLMEIHQLLVVMISXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNSICLWDIKTGQQNTRLDGHSDYVTSVCFSPDGNTLASGSYDKSIILWNIKTGQQKAKLDCHSNCVTSVCFSPNGNVLASGSRDRSICLWDVKTGQQKEKFDGHGRSVDTVCFSPVGNKLASGSLDKSIRIWDVQTGYQIERFDGHSDRIWSVCFSPDGSYLASGSYDKSICLWDVQTGDQKEKLDSHSDRIWSVCFSPDGNTLASSSDNSIRLWDVKNEIQTLKADKTYRPFQNNPLSLSKTITILRISQTPLFQAKGALIFKGDFVNYEGFDLRSLFQSKGSCFIEDLNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P4U3</accession>
    <name>A0A8S1P4U3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1060009</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P4U3"/>
    <dbReference type="OMA" id="QNTYVSH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF0">
        <property type="entry name" value="UNC93-LIKE PROTEIN MFSD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="413"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8098019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="49776" checksum="04944FEBAAFA7C9C" modified="2022-10-12" version="1">MKQNANLGKVLYMNIVLTFHLSQCLALASLLPQIVDQNNYSYLGGLGLITIYTVNLIFNLVAPLYLTKMKYRYGFAFQAGLVLPCFIPAYIVSRCDGNDIQNLICNPILLIPLTLIGCLFLGAGLGGYFVLQNTYVSHCSTEKNKELFFGITYMLLGSAYLLNGVTSDQLLQYIGRENFFLYSGLFECILSLLFIFVQQPDKRNQTQISEANQHMIQNDSVDKLEEKVTNINEQFAKIGFKAQIKQILQKFQLKEMKFLFSLFISTGVVIGFEFGIFHKFISASLPGEDEITINVKTARIFLFVGVAQILSGICNGLSRYFFGIIQNSIFYGNIFQCLNIIAILSAFQKDYNINIILGFLIGFTDNSGQFNSAVIISDIWKEDMAIFGLYLFCQNFGVMFTNLVAIFLDGQYLIYYLILLITLQCSTSFSQQQFKRSLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6T3</accession>
    <name>A0A8S1P6T3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1070137</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6T3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000134">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="30"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="94"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8098631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="351" mass="41131" checksum="FA72FBBC8A21B112" modified="2022-10-12" version="1">MFCNSPMVQKSYQSSSLSYQQQIDDMSCIIDNGQTGSIYLGNIESASSLENLRRHKINGVLSICMNKIPFDVQKQLKNYQHIYLDDCESENITRHFDNSNQFIEKARQNGNVLIHCMAGISRSATLVAAYLMKKSNMNAQDALKLLERKRWQVYPNNGFLRQLQQYERALQQQTNKNEIRDSSLLKESLLKNKQFQTPIKEISFINKYEEQQQYSGIKTRPLDDINYQNYKRKSLGIQFYCSPEQSQKIQFSLTNHKLNSAIKTSPNFIRKQNYLQDTIHGNKIEGLASLELELNSLDWQNKKQGFEQKFAKQQTQNKNKIFIYQNKPLQFNQHQNKLDELLNAFKRKQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PA64</accession>
    <name>A0A8S1PA64_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1100102</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PA64"/>
    <dbReference type="OMA" id="PADCFFF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IPO4/5-like TPR repeats" evidence="9">
        <location>
            <begin position="102"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Importin subunit beta-1/Transportin-1-like TPR repeats" evidence="8">
        <location>
            <begin position="453"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8099711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="865" mass="99314" checksum="08D564E2D284790B" modified="2022-10-12" version="1">MQPQQFLDLVQCAFFDSNNQRGQAEQVLLKYKDECTDEFLMYCASAFANRAIQNRLRIACSTLIKRLVGLVHPSNQIMWLACTQNTKNEVKIKFMDQLIDPEHEIRRSAANTISEICAIELPRQEWPDLISRLTTNSKHSNVLVKVSAIMTLGYICESLKNHQSSGISEQDAKVILMGICVGMDINEQNVEIRITAIKALQDSLYFMNNLFTQQEIFTFVKNLILQNAMSNIQEIKLKSLQCLIDYVKQLFAFLPVFISDLYQTTQSAFENQGEVTIAAIEIWNTICAEIKEIITENTNQQTPETNSVDCCVQFFKKNYEGFIIPFMRNLLLDDGDAEDEYQGLSVPNSSCKGLAYIIEFAGNNTYDSVKNFIQNTITHQQWEYRKASVMAFGALAEVQTEEIGILIKSALTSLFGCLVDSNIKVKKATTQTLSRIAENYPQCFHEHDQAQQMLVTLLDQLSNKISIVQNLVWVFVYLTEGLANFQNSVFTREKFTILQHLASTSVRPDIKNSEIALIDTAFMAILNIIYSVTETKLCNDYLVQFYQQIQLLENSNQVPIEIKYHLEMGLMSAMHGCVVRLDDTTTSEKQFEDIMKTLSKVDSRVKNDYFYVLSGIAYAFKKKLTKYSTQIISELNKPLSEPEDMESFKTALFCLSDIARAMEEEFVPYMNILNYFFGLIKNGSFNRELKIQVYNAIADIILGLKDKSTPFMNDLKEILKLGFAASIDLTKSKLSVDQDYAERLKETMTAFYTCMLHAYCDPKTPIFELGDTVQWFIIFCQEMCNLKLKPTIEYVRLTLCCIFDCSHFFQNLPDMKQKIKEFITSDFVIELIRKLSQFNDKDYQDCVSFAQQLLNDVYGYQLKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBP5</accession>
    <name>A0A8S1PBP5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1130009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8100617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="16938" checksum="98CD0C0F0FF8A5E4" modified="2022-10-12" version="1">MVIQHCLQDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAKFWDAKQLTYSGEYKNGKKVGQWDIEYRQDKDKPLFKMLSGGGSYNEKGDGVKIGQWIELKGKSKNVTNNGEYQKVKKLVHGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCL5</accession>
    <name>A0A8S1PCL5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1140039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8100960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCL5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8100960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="78" mass="9291" checksum="D1320252AFA0FE0A" modified="2022-10-12" version="1">MQHTQINIELYKRMNKNTQNSHNGGQGKITKIIWKKDVSIIKIKKFSVNAIIDCDTIMKYKQRIKQYQRIFIVLQVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PEX0</accession>
    <name>A0A8S1PEX0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T1160134</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PEX0"/>
    <dbReference type="OMA" id="ERCNTKE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001191">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="169"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="50"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="296"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8101790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="418" mass="48259" checksum="FCE12E9156982FC7" modified="2022-10-12" version="1">MKRLSSQNKTTRSSQVISNGIYFGQFQNPQPLLTAVSNTQLKPTQDLRRRNFIPKKRLQSPKILSPRQQNTKPIKSFFQTKPSPKASYKPPTIIVTQPTNRSTVIRIRSPIKRNQQPLISQEMDNKSIELLQELANSQLNRTSRKKRRPQTAGGYTIPSQHFSNNIENYLIQEQIGQGSYGIVKVGLNLLNGQQVAIKIYERIKIISKKEYIRKEIQILSSLQHPNIVQLLDVIYTDTQVQLVMEYVGPLSLRAYLKQQPNKLIPETEAKNIFLQVVKAIDYCHSKNIIHRDIKLENILIKDNKIKLIDFGFSSFIEHKTTSYCGTPSYMAPEIILKIDYGKPADIWSLGILLYVMLHGKFPFQGKEQKELFSKIKTGIFSSNGISQQAQRLINNMLRVRSYERCNTKEIIKDQWFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHL0</accession>
    <name>A0A8S1PHL0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1180153</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHL0"/>
    <dbReference type="OMA" id="FVFFDHN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000526">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000587">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000771">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000660">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="300"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="336"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="452"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8102524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="57404" checksum="3D5ECA939557EB9D" modified="2022-10-12" version="1">MGEKRIDKYLFNPKDLIGEGSYAQVFRGREEKTGVKVAIKVLNKSVINADDYLREGLISEIKVMQKLKSPNIVQLMDVMETNNNYYIIQEYCDSGDLDKMIENYVSQKKTMPEKDAVKFMIDILNGFIQLIKNGIIHRDLKPANILIDKGTFKLADFGFAKCVDNFKKDMLASLVGTPLYMSPQILDNKKYTSKTDIWSIAFIFYEALFGKTPWTARSPQELLKNIRNQPLQFKGPQISKEAQEFLTGCLQAEEKDRFSWDDIYRHPLFKGHFTDFIKNVSILEDKATYLINDIRQMIIKEQLDIGELFAELDMTKDKALNVNELGKFLTRVDKNLTREEIEYIFNKFDDDGNNLIEFNEFKKWLEENDCRMTASEVSRTKKKVSILLKPQQDIQVGSLDDRARIVIEKLKTSIVKYNINLLDLFNKYDKSANHELDVLELGKLLKRIDSSVTDEECKSIFSFFDHNKDGSVSFNEFEFVLKECLVKQKIKQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIW8</accession>
    <name>A0A8S1PIW8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1200015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIW8"/>
    <dbReference type="OMA" id="HSESITH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052798">
        <property type="entry name" value="Giardia_VSA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23275">
        <property type="entry name" value="CABRIOLET.-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23275:SF100">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="673"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="848"/>
            <end position="887"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1178" mass="138624" checksum="3B93AB3E0EF1335F" modified="2022-10-12" version="1">MILKSNQNQNNQNMNFRSIVKLLITTILYIVQGCQFTNYEESLLSKKYQIETTIIDFKGLTKSFAFWSFCIPAWEVDSYPQVEEFMFEDIRNQQLLFLINAEQNEQSLIIFMYVDFISGQNEVIHSLHINTKLQEKVYEYKFDSKIYEGKWYLSMITIGSQFIKFQTSESNNYIDIHDEIPLFNKFQIIIGGTGFVSHKYQLGIFRGRLSKLITIEDEANQNLLWVLSNCYIPINMIGGQTIHLIDDVQIFKGNTQLIREIDQVGKSFRFFCWVKYDFSEASFTTTYLLLRLTIFKNYGDELRIGDELAKITIDLDKSQPQNCGYDVISHMYSTPKFGPINEQFQQKLNFRDNGEYFYQQLQQWHIVSFFYRQTNGPSVTFNFISSNKIIYEKFPEEYAQGLFTNTKYYAIFGGDRTIQQKLRGQIAYAKFEYNFQENTELNIVCHQTCSQCNGPLSTNCLECDSQKNRIFSEDLKICSCQNNFIEYQGECKSYSQIYSNIQILNVSQVQNNLICEYGYFYLPTINECIKCPQANKVTILCTDCLIYPNTWYLKPVCTKDFIVDNDSEKSAYRLEQRSTFNYDVYFIDQDANLVLINGAENYCNGENDLPNCFNIEKQTHLFQTFYIMCKQDYYFENNKCLKSVDHCIQSDYRFQICLQCEEGYYLYNNICIICQNLCTKCHLINYYYKCLQCPNGYEINDNQGCTKCGDNCDICKFQQMQYSNQKILRCLKCVHDQKYYISLDGQNCFENKIQNCLYAFEVSRNDYKFNSLEYELNTFFIGTVSICRQCQEKYTYNINTNQCESQQSDECYYSYSYITPPQTLKEVCLFGPKINTIIDPISFITESHCLNYNCHICMIRQINIKVSYICLECNNGYYAEKLSGYCVPCPQELRCQVCYQQNKISKDNWKNQVRAFYRSIIDDDLQSHSFIEYGLSQDIGDYEIVCQFCIDGYQLHKGKCIPVCPSCCLKCKIINDENICIQCPQIQGKRSLSVQNNECIQCPAFCVICRIREQEEIKLINPVFNNQDFYYYSNQCLQKQVGYYDKDLKMYVDCPQGACMKELQISLILYCQKEEYDKEIQSLKNEEDVKNFKQSNILIDDLFSNSSFPQFEQPEFYQMANEQVIKSIIIIIHSLKPQNCIITNNNKSIKQKFSQNIFSAIQKYLIGTIWKWEYNIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PPP3</accession>
    <name>A0A8S1PPP3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1270021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PPP3"/>
    <dbReference type="OMA" id="MIINVIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="58803" checksum="7FB928EB658C4CC3" modified="2022-10-12" version="1">MHKITLKFLDQSIEQKYQQEHQIPKRRTHLKIFMLFFIVLQIIKLTIALIIQNYDVAYPILGMMGCTALSKFFNFNKEYHQRALIIYINICFSIYVLFFDPPSDTPTMYFRGAHQMIINVINILGTEFLDSTMTITVLYSLRIFHLVKNSSSIDITSVIMGLGSNLSLLVIVYLYHKAIRSQFLLTQINQRWENILKQILHNSKFILINFQIEKLQFQSITSTFSQTIQSKEEVLNFLRESKVENDSLEQYLFQKLQDYSQNYQEIINHAVNIKFNKQIMQVDFSIFFGNQPTILIQTRQSKLHSQNQEIQQVCQQYLKLLIIFIRIIKENIPFDKIQFHNIANKIQFQDLMIQIWNSQLLCKTISLKKSLQKIQKYSNPNTTIQLVSPNYFINTIPKIFYLLLAFIVDCQTSELIIIKGETLDTNLNKIKLQGKFNIRKLNYYISKIKFYFLLICKEINAEQFCINLEFNDEIFSPFTNIIMPQLNFGYLNQNFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTQ8</accession>
    <name>A0A8S1PTQ8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1290019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8105968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTQ8"/>
    <dbReference type="OMA" id="CNIFFQP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="40782" checksum="A6EC90BA30617CF3" modified="2022-10-12" version="1">MQLQQNQIQQEEFCYELQPQFTINQKEQCNAIAFNQNKQLLLVAYDKNIKVYQFNNGLLILIQIIPKNRYNITILNFFSYNKYTQFISGSCDSVIIIRSITQLRNQKYIQKLNSHQRGICCLVIGQDQNMFLSGSVDQTIKLWSCFQQQQISCQWSCQQTIIDHTNDVYALDINQQQTKAISCGYDDTMLIMDIVKYMQIYKLILKQKIKLSNFGYRILFLNDQDFIFQSYNAKFLSLFQLNQNQQYVITQQLPVQSLSQQCNIFFQPQLIRSKGFFACKNSQNLNFIRFNSISNELELLHSIKLDNYQQYGQLSEDGEYLITWDEKLEQLQIRKYKTIHMQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUA5</accession>
    <name>A0A8S1PUA5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1310113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PUA5"/>
    <dbReference type="OMA" id="QTKETLW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8106686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="301" mass="34795" checksum="7CC814B843BFD959" modified="2022-10-12" version="1">MQITPKALTALQKLLSVNSPIYPNEIRNQEHSNSQSNQKSQQGQSSCQQNGIKNSLKQKFMSPQDGIESLLRHSIGKASLNGKPQVNVKNELINVLKQNQNVKENTIKRNGNSLNQLLQPQINSPQKEQSEKNDYKKKRPPQLCDIVQDDDNNVQQKKIMTSKSLQKSPNNINKIGSGFFTSTHQKQTHMNVFGSLNECLRQYIQQKSHKRQNGNDKNQEEKKVNENKFDSAQNKRISLSLQTNHSNLLQELYLKTSKILTQYQTKETLWKQQKNSLKEEVKFLKQLLQQQQQESNQKYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUL7</accession>
    <name>A0A8S1PUL7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1320104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8107017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KCESITC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
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        <property type="entry name" value="EGF"/>
        <property type="match status" value="32"/>
    </dbReference>
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        <property type="entry name" value="FU"/>
        <property type="match status" value="41"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="3383"/>
            <end position="3406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3470"/>
            <end position="3489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="3495"/>
            <end position="3516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="3536"/>
            <end position="3557"/>
        </location>
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            <begin position="3563"/>
            <end position="3591"/>
        </location>
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    <feature type="domain" description="EGF-like" evidence="2">
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            <begin position="144"/>
            <end position="176"/>
        </location>
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        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
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            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="486"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="541"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
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            <begin position="592"/>
            <end position="638"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="639"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="840"/>
            <end position="885"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="893"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="991"/>
            <end position="1032"/>
        </location>
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    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1040"/>
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        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1084"/>
            <end position="1113"/>
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        <location>
            <begin position="1229"/>
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        </location>
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        <location>
            <begin position="1271"/>
            <end position="1318"/>
        </location>
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            <begin position="1326"/>
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        </location>
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        <location>
            <begin position="1373"/>
            <end position="1412"/>
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        <location>
            <begin position="1413"/>
            <end position="1460"/>
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            <begin position="1510"/>
            <end position="1559"/>
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            <begin position="1612"/>
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    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1774"/>
            <end position="1812"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1820"/>
            <end position="1861"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1862"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1917"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
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            <begin position="1964"/>
            <end position="2003"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
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            <begin position="2004"/>
            <end position="2051"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="2101"/>
            <end position="2150"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="2158"/>
            <end position="2195"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="2203"/>
            <end position="2238"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="2246"/>
            <end position="2283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8107017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3630" mass="415572" checksum="318359BA9D797394" modified="2022-10-12" version="1">MKQNFCPDLHVMVPDMDLHICVSYAEICKDVPEVDILVAQNGTQYICHPRFQPFAPGTPTIDFFGSINCGYEEGVQMVIEQLSDYHLKFYCKFIEGENYKNCLATEIVNGYQRCAYCKAPFVGESCLKLTQGFYDWRYFNPPKKCESITCIECDNPSSCTKCAPGYGSTTPANTCDKWLDSTPPCKEGTYLETVDKCFFCPEGCLSCRKRSASTQPSVTCDVCDSRYFKVKCRQDDPDCYFCFIGTVCDFDRQQILTDYTTLNALGQYQYVISCTQCDLGFFYSEEKKKYSENIQSYCLIYDPIIKKCMSCKPDKILYYGSCSNPWCIPDCKTCSILDPNFCTTCDSSLEKGGVIKEKGICSCKQNFGLKNDVCEPCSDGYCQECEQDDFYSCTSCALGTNRILVNKQCICQPGSFEPEDGGLICIVCDWSCQTCFGPTNYECTQCPDEIISNRIQVGNSCPCKFGYSDYAIQEQRCGKCHPRCQTCFQAEDDTINQYCLTCIPGQNRMVNDNFNCDCIENYGDINGTKDICFICHYTCGNCDDFGSNNCTYCSDSSFRYLTTSRECLCKTSYYDDGTDNIECSKCHYSCLTCANSTESNSCIECPSTRIPSDSSAIQFECPCLSSHYYDDGLSPTCIECDVSCQTCFGPLSSNCLTCDPIYRQLDLSSCICPIGYYDVGQLECEKCHYSCFQCFDNTAEGCIACSIDLHLRVVKGNTCKCIDGYYEESGTAKCKKCSYKCETCVDQPEKCLSCPFNSLRILDIDKGCYCAGEYYEKENEIFCQRCHFKCKTCDAFSESSCLSCDLIAFRELKLKQCKCQPHYFEMEVQECAICSAYCYECINNFDNCTSCQDDRYLVGSTCKCSSKFEGAQISTFEFNGQVQCQKCHYSCGKCGGVEENDCITCIDTDQRYQVGNTCVCKEGYFDAGLPVCQKCSYKCKGCMKQSEICTSCQDNTLRQLVSGFNTCQCIQRYYDDGQNEVCQKCHYSCLRCNDIDTKCELCSIESNRIYNDQLFTCDCGVGYYDTGVEICQKCHYSCFSCNSGDSNSCNSCVDSMISNRVFYNNSCKCLFGYFDDGQSIKCQKCDIQCLSCINQSYQCQSCPQTRKIESNCKCQKGYYDVGLQLCLKCNSNCFTCQSTSNNCTSCDSDQFRELNSITKTCDCQIGYLEINGICEQCHSSCKACSQSLNLCASCGQFRYLQNNDCICIDGMYESIVDKQCKLCNKSCLTCAYSDIYCLTCSIDNFRIFKSGNKCECKQGYYENPITQQCEECAKSCLTCSITYDNCLSCDTSLNQSMINNKCLCSQSYYFDSLTKSCQQCHITCLECQNNSECTSCRQTTRHLDEDQKKCLCNDGYYETNQSNCQQCHLSCETCVTINTNCLSCMSIYQRTLKNNKCLCIDGYYDAGIELCQKCNNTCKTCQNSASTCLSCYEIEQNRYQLGDKCLCKSGYFELNTNICSKCSNECLTCQGQADYCTSCDINSKRVDQSIIHKCPCIIGFYQDQNQICQKCHNKCQTCINQSDQCLSCKFELNSNRNSLSDQCNCKEGYFDDGIQLQCQKCNLKCKTCLNDSINCVICSNSIRINPPTCNCMDGYYEDEQFTCQICAYQCSTCAISPQNCLTCKPERIGNDCKCTDGYFEFGXVDSMTSNRVFYNNSCKCLFGYFDDGQSIKCQKCDIQCLSCINQSYECQSCPQTRKIESNCKCQQGYYDVGLQLCLKCNSNCFTCQSISNNCTSCDSDQFRELNSITQTCDCQIGYLEINGICEQCRSSCKTCSQSLNLCTSCVQFRYLKNNECFCNDGMYESIVDKQCKLCNKSCLTCAYSDIYCLTCSIDNFRIFKSGNKCECKQGYYENPITQQCEECAKSCLTCSITYDNCLSCDTSLNQSMINNKCLCSQSYYFDSLTKSCQQCHITCLECQNNSECTSCRQTTRHLDEDQKKCLCNDGYYETNQSNCQQCHLSCETCVTINTNCLSCMSIYQRTLKNNKCLCIDGYYDAGIELCQKCNNTCKTCQNSASTCLSCYEIEQNRYQLGDKCLCKSGYFELNTNICSKCSNECLTCQGQADYCTSCDINSKRVDQSIIHKCPCIIGFYQDQNQICQKCHNKCQTCINQSDQCLSCKFELNSNRNSLSDQCNCKEGYFDDGIQLQCQKCNLKCKTCLNDSINCVICSNSIRINPPTCNCMDGYYEDEQFTCQICAYQCNTCAISPQNCLTCKPERIGNDCKCTDGYFEFGSTFCEQCAFKCSTCSTQAMNCNSCKGNRIQEPQCICQPGYFDDQINEDCQLCDSACLECNIKGCLSCNANRILNEEMDCVPPPNSIWYNDTPWCSTCEVAVVKAYLSDDISKIIIHFDFPLNSKGFNQQFELNKCLQLFELKSLQSFGQNSQCFINPDNNHELLISLGENSKINVGDEILFISNSLSHINCETTLNKFIFTTLQMPIKPFAPQVEYHVPLHQLNPYAENSVYLNSIRNYGNRKLDNIIWNFQVETNQDIQALSDFIDQINFLQEYNLLIPKFTLPSDAILKFKIEYQNFIHIQSYSEFIIYTHSGNLPQIDISVKPSYFVYETITIGLSAGTLFRQYQVDNSKYMVQLSEINRHPKKSSPSRLNTSLETNPFEKIQANITKYTLSPNSTYTFQVIATNLNTNKVQYQNLSIDIPFAGLICQFNNRGIQSIRKDLNLQIECKDLDTKYDWNNDPDLYIYVTCKDLTLNNSCLNQQKQMINVNVTDSVQFIKKNTISVFTVQEWSVTVIKFQQTQKFNQIIVYLEDDFPVLDLEFNQGYLMRKVNNYEQLNFTFLIPFNQKPFLLDLSIAIIYNYQIIEILQPKYISHQFKLFNSIKELNFGDSISLKLTAQYTNNIMPSLNTIKLNINQPPFCSKLIITRSNDLALTNMMVATSCEQSNDSPYKYQLRVFLRESDLIDFLKGSSDDSLILYSFQYQTQFQIQIPTSVDSSKIGLLLQVLDSGGSITNIYEMITSKPAIINCSQIQFQNLKLQNKLSLLFEAMNQKCNDLHYQIYLDLLYQQILPDQNHNMLKFQAIKLYKQFLIQYGQSKPQTRLLIEANQKKCYDLNSSHLFITQNSSESDVNLSKKIDDLKENIHNLDIVLNYFSSIKQQCEENLKIHQYLWDEEIFQQYQNSQDSLINLLYFLDQIYSDFSQVNIINNVIYEKISELLQFITLIAEEAQNTIIVNEKPLIIQGKEILWQMKRRTKQIFNHQFNIQTTDEDYLIDFIQYESTYLKTNPIRFTQDLDNILQSQFDDKTIKIFLENYYSIKLKNLYHSRFLIFEDFSASYTLKFGSYQVCSENSVILQKYQILCIIKTTFGKFYKCILKSQLIDDHIELTCECNKFGEIFLTTSTNFSYGIENQTTQSIFQLDIASRPEEYLVLIIFTSSLTFFFMATYFINLFRDQKNKEEKLDTEPNSINSPHNLNNKSLLYSGNFKLFKQKFKFIHQTVSLFYYKDKNIHISYRILDVVSQFNLQLTLVILECFLLNNQAIFICIFIITNPILIFILRILFKIMEAIYRFGRIASAVSQSFLIILLVLPNLILIILYQYSIILKSEYYVFYIIYLGNTIISQVLIEPITIFIRIYIYRQISSSLKNMDLNPKYHLMHFFIIHSCLEDIFEEFTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYK1</accession>
    <name>A0A8S1PYK1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1340042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYK1"/>
    <dbReference type="OMA" id="IQFIEME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="139" mass="16252" checksum="B1F5B5A330B7216F" modified="2022-10-12" version="1">MNQFESTEARILYLIYTLYQNNTISLDQKGILKDYLITKENGKFMNAITLFEKSKDIDQLQQFLIQFVEMENSISDFVSVNSFKTIKSQKPCDLFKETTQSSNANSNPYILFSPIMNQKKDGDTQFISRKDRKYSTQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q029</accession>
    <name>A0A8S1Q029_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1390048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8109027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q029"/>
    <dbReference type="OMA" id="INCVIMN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="246"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="341"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8109027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="513" mass="60001" checksum="876C02DA9AA81F1D" modified="2022-10-12" version="1">MLQSFASNVNCPNHNQPITYIDTKKDTPINNRAKCLNCIPLNDAVQLKIALEKFKKLKEDEKQQIRNYRKNKIYDFLEFKKVIHQLSESISQSLNEISKQINERIEKIVSVIQQAQSVQWQFDINEYLTISGLQNIGLQLSQQGNEFSLIKAGYSVVQMKKEQQLQTTLSNYQNNIKSIQSNMEILKESLTTNLIINEEIVGKAPQKEKCLATAFNKDNSIMISTSEELIKVWAFDNGKLELFQTLKGHDKEVTCLYFSRKNNWFISGSRDKTLRIWKEFSNSEWGCIQILEGNSFCINCVIMNQYEDQIISSRDDNKIKVWMQESLLTSQQPQWKCYQTLQDHKKDVYSLSLSSSGTMLVSGSYDKTIIIYDMDDQQQWRLKQVISNDYENWVHSVCVINDKLIATKLYNGVVILYQYMESVESFEQIQELELSKSKMSYFDFFHMFYNQQLQSIFCRHLDCIYILFQQTTGRYGVQQKITDNYRGGSLTDNGEYIALWNNSTKQLEIRQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2D9</accession>
    <name>A0A8S1Q2D9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1390051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8109033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2D9"/>
    <dbReference type="OMA" id="AYYFVKN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="69"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="189" mass="22954" checksum="25519115E1DD0E4F" modified="2022-10-12" version="1">MSKAYLAVKNFKWSEMVNLLAYGVKHPDTLSQTQRITRLYRATIRRHYTSQLENFKGDHIRFYNEMQVAQKDFNRLQELNNNYANLNQEQKVEFSQLENKWVNWLETNYDAFLYVDECRPYSSTSTRYIVYTDEELKFDPLGFYKQRPVNDGKFDPHLPYFRDYPYNESWWNMNESFPSDFDDTPSQQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q369</accession>
    <name>A0A8S1Q369_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Group 1 truncated hemoglobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1410070</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q369"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00454">
        <property type="entry name" value="TrHb1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044203">
        <property type="entry name" value="GlbO/GLB3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47366:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_37, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47366">
        <property type="entry name" value="TWO-ON-TWO HEMOGLOBIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8109604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="14951" checksum="A12DC8CC5A305F13" modified="2022-10-12" version="1">MDNKKPLYERFGGDQQVSELIDQFYYKVLFDKLLRDKFLKADMSRVRYQQKRFFSQMMGDKNTQYTGRDLVEVHKDLNITNQQFDKFKVHLKNIAQDMEVSGADLEELLDHVEKYRDQIVFSKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q577</accession>
    <name>A0A8S1Q577_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1440023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q577"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="97" mass="11373" checksum="E806CD4555E0F1FC" modified="2022-10-12" version="1">MDEIQISCLKRKKESKQKMVEAFVTFGFYRKYHNLISAIRIKSLNNQTNELFQKISYLISSSQIGRNIGIGENETKIIKNRINYQQVLKRGDYNLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7V8</accession>
    <name>A0A8S1Q7V8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL6 alpha-beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1470078</name>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1470081</name>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1470083</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7V8"/>
    <dbReference type="OMA" id="SARWACH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000008">
        <property type="entry name" value="50S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000004">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_uL6_CS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="5">
        <location>
            <begin position="12"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="5">
        <location>
            <begin position="118"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8111363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8111369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8111373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="196" mass="22374" checksum="752B771064CD3CC3" modified="2022-10-12" version="1">MKYILTEEHVTIPEKIEITTKSKQVKVKGPRGELIKNFKHAALDIQVQKKVNKKSNTTKSRVSVRMWQSYRKQRCQVNSVASSIKNMIRGVTTGYKFKMVLAYAHFPIIINLLEKGMGVEIKNFLGEKIIRTIQCLPGVTITRNETEEKNVLTLQGNDLNNVSLTCALIHQACAVKNKDIRQFLDGIYVSEKRLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8S7</accession>
    <name>A0A8S1Q8S7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1460051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8111010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8S7"/>
    <dbReference type="OMA" id="DIIFIVW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="39811" checksum="D292C737775A9CED" modified="2022-10-12" version="1">MIPSTRICIAPNCQASSRLIQSIKELDYHKGHSSSSLGQLTKKLKDVIPEFPITTSTKQIIESKFQIIQEYLQKKQEIYQKKCEKYELIEAASKFINSEYSLLNERAIDLIVKALPNNISETLELQTTQYCELQIQIQKELDDLFLLISADTKQESIQPVQQVQSPKKKLSQIQTAAIPIAITEKPQFIKMPQAYDQLSKFEIVNQKAKHLEGYPSFVMTNQLLKEGSQIRFEIVKIQNDYVGIGIGAMERASIEKNGLILTGEQLQTQGHGVYMNFNDKCIYHSEIENGAVQGKDKGISFQDGDKILCTYSQGQLTFQNLKTNSSYSMKINLTDIIFIVWLDLSEIKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCF1</accession>
    <name>A0A8S1QCF1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1520090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCF1"/>
    <dbReference type="OMA" id="DCKQIGG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="121" mass="13674" checksum="66AB4745E368C806" modified="2022-10-12" version="1">MIQPKVLTQLLTQCQNEFKVQSIFVAASIDGQMIAQVGQTQHQLPQFSTLSIFFDDCKQIGGIQLSQNIKLNYVFVEIEDQSIVLSNFKNLFTLGIASSQNVGLLVLYMNKLHQQLEKIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCI5</accession>
    <name>A0A8S1QCI5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1550105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCI5"/>
    <dbReference type="OMA" id="QNYNSRK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="290"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="447" mass="53287" checksum="79049B5F3678014E" modified="2022-10-12" version="1">MNFLNKIFASSHNRSSFQIEDVSGSEEDQDEIPEHQEIPQFKKQNIQQLQNYNSRKKPLPKMGQADFLAKYDENFEDVFGDKPIFNDQHKGKKRRQEEEDDNDNENDNEKKTSKKKTQSKERKQQSKEQKESQKDNKKTISKEQQSIMDYKDVQLTIDYKEFNDMKEYKEFKEPIEPKIQNKPNIRLPKFPKQRKRIANNHLSTLRSNSDGRDSVSNKDRESIQKNNKYNPNEIALPETTLPKSEKKQDKSKTMFFTFGLNNNNDDDSDQEIHQKINDLFQDQKEKQQDQNEQNSNNQISIKKKNPEKKQKNEEKKEKNEKSEKKQRKEKKEKEKQTINGFNDIKEILRSVNSKQNSEQRETRKLNSEQRDKKRGQKQSRENDDKIITKSPSPEMKLIQYETQKRVKRSQKRVIEDLEMLNVDELSLNPNIVECKPNLYLDYVPVYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QEY8</accession>
    <name>A0A8S1QEY8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-related protein Rab-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Small GTP-binding protein rab1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1250084</name>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1540055</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Protein transport. Probably involved in vesicular traffic from ER to Golgi.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QEY8"/>
    <dbReference type="OMA" id="CSMARDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01867">
        <property type="entry name" value="Rab8_Rab10_Rab13_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001018">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8104815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8113140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="210" mass="24434" checksum="FD5F667CC57B4682" modified="2022-10-12" version="1">MNSNSDYHFLFKVLLIGNSGVGKSCMLMRYSENQFTNNFYNTIGVDFKTKTIALGEHNVKLQIWDTAGQDRFRTITCSYYRGAQGIIIVYDITDRESFENVKTWMAEIDKYASESVNRLLVGNKADITERREVSYEEGLELSRLYQIPFYETSAKSSINIEAAFTHITKNILNRELHNTRAVVRKTSNLRLQNRQQQQQQEKKKQEDLCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QG77</accession>
    <name>A0A8S1QG77_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VPS10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1550023</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QG77"/>
    <dbReference type="OMA" id="YAQMINI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002201">
        <property type="entry name" value="Sortilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.270:FF:000002">
        <property type="entry name" value="Sortilin-related receptor isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031777">
        <property type="entry name" value="Sortilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006581">
        <property type="entry name" value="VPS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF27">
        <property type="entry name" value="SORTILIN-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15901">
        <property type="entry name" value="Sortilin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00602">
        <property type="entry name" value="VPS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="698"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="4">
        <location>
            <begin position="43"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8113365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="750" mass="86467" checksum="F09F1E72850F1EF1" modified="2022-10-12" version="1">MLILFIIGVSAVNVTLFQADSALYSPQFCLMRNSNLEGKDWLETIFALSEKGRIYKSVDNGYKWSDETLILEKMGINDWFYEIQTSPANKEVVYIFGKNGTGIRSEDCGSHYTWFTFPSSFSDFKLNSMDDEWMLAFSNCERCHPPFNKEVHKSNDGGKTWQWILSSAMMASWDKLIDSELIPDERIVACHLEYNQSRVTYSDDYYNSYKIIHNNGTGFFQTQFHMFVISKFQSAYRLHVAPAFYDYAQMINISIPFDQQYSYTLLDTESESIFLSISHNQTNKKLTNIYTSDFQGFKFTISLLNNVRSLNTGQCDFEKIESLKGVYIANIYDHEKLKVVKESDLEKYKKTVITFDKGAIWHPLKAPETDVNGQQIHCSGDCSLHLQGRSVSKIYRESKAVGLIMASGNIGLYLEEKTINTYFSRDGGMTWYEVRKGHHIYSFGDRGGIIVMAKAKSLTEELLYSWDEGLSWESIKIPKMIVTSITSRSLYINFIITGIDEKRKGIILNVDFTTLHQRICSGIWDPLDPDSDYEFWIPKNYENGKCLFGQKFRHTRRKRDAKCFNQQSYDKLQYIDSCECTIEDWECDYGFYHKIDGGPCVPIASLFEEDQYIDILKPPENCTGTYMKSQGYRKIVGDFCTGGVDLDPIETPCEDGLQQSLSNQTQTLAQNSIKKEQIFNETVQVEMNVNVGIQLNNSIYIGILIILIAFFGFVFLKKMKAKENLDMKKDYYSNFERGERISLREDDDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKI5</accession>
    <name>A0A8S1QKI5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1650100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKI5"/>
    <dbReference type="OMA" id="TNQNCEL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8115674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="270" mass="31545" checksum="0C0E636D94512E8F" modified="2022-10-12" version="1">MSFCQEPKVDCQYLKSVIKNLNNRLYTLENANQNYEQKNNQVMEQITEDLVIPKLKLNKIQIGFIENNIPQKKVLVTFGNKENFRTDNCDQVGDRIDQNIFTINQPSPQRKNIIQSQLMNQQLQFLKQKDDKLNNINKQPKLKLVKQDSNLIHPIFSKKNSDQQSIGSKFYTRNNSIQSTPVKQIAYNSSLGTTPLRRKNSQKNSDLFINQLQIDDMMHNQSKFLKYFQQNYGSNKLSNTFDNVNNNLQRRNSLKSQNKITLIDAQIIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QP75</accession>
    <name>A0A8S1QP75_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Golgi apparatus membrane protein TVP23 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1710034</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TVP23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000180">
        <property type="protein sequence ID" value="CAD8116357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QP75"/>
    <dbReference type="OMA" id="FEWMIVA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008564">
        <property type="entry name" value="TVP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019:SF7">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05832">
        <property type="entry name" value="DUF846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8116357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="218" mass="25103" checksum="E2C1B91E03BD4C0F" modified="2022-10-12" version="1">MEEDLHFKIQMQEMPSAPTKPLIQTSTDEPTKLSVASTSESIPLQTSNYDLNRASHPKACIFTFLFKALALLFYLIFGESIWSFILVIIFSAFDFWTVKNITGRLLVGLKWENIILEDGTSKWEFYSMPNKQINTVDKTFFWTAQLGFTLAWAIFTFSNMISFTLMKFVIDIIGLSLCWTNLFGYYKCNGDHKKKMKDLQGDITNKVVTQFVGQMMSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPG5</accession>
    <name>A0A8S1QPG5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1990001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000208">
        <property type="protein sequence ID" value="CAD8117436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPG5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="218"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="260"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="302"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="344"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="354"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="386"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="428"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="470"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8117436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="587" mass="65900" checksum="CDC3CC971FD7D719" modified="2022-10-12" version="1">MNQTFQEKEFLADIFAQVKGVDQQIFGVTLEIFRKEKVQDCIGYLSNNGNQRHLEQQILQGLNFSQIKNEQNLYVVRNDMKQMTDVLIKLIDHGFNNKNYSSEGYFKYIQDLIISINNNRQIIEFLQFIVQLTSIDNNLIQCGSNSLYILVQMKIDIRNKNFENIKIQNTSLIGSNFVRCNLSGSEFDNVNINGMNLNGALLFNCKWKNLKIHELNKLDGHSECVISVCISPDGNTLASGSHDKSICLWDIKTGQQKAKLNGHTEIITQVCFSPNGNTLASSSYDNSIHLWDLKTKKKIAKLDGHTWWVISVCFSPDGNILASGSQDNSIRLWDVKTGQQLSQLDGHTNCIISVCYSPDGTTLASGSSDKSIRLWDVKTGQEKAMLNGQSDCISSVCFSPDGYTLASGSIDNSICLWDVKTGQEKAMLKGESDCIYSVCFSPDGNTLASGSQDNSIRLWDVKTGQQKAMLNGHTNNIYSVCFSPDGNILASGSKDKSIRLWDVRNEKEISALDKTYKDIFAQFKTLLFLNNPFSDFNYSILVISKDIILETQGTFFLEGKFINHQGKDLTSLFQSKKSYILQNIILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQ50</accession>
    <name>A0A8S1QQ50_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T2100004</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000219">
        <property type="protein sequence ID" value="CAD8117776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CNNKVNH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035855515" description="TNFR-Cys domain-containing protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="1745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8117776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1745" mass="198097" checksum="1B5009AE86ADA6CE" modified="2022-10-12" version="1">MSVSIIFILIALIDVTNQQWKILYAYFIDDFHFSEVYIPSTFKCANYITCTKYPTKLITLNSTYPSAEYYHNLMFNNGYYFSVDLYFQGTWSSETVTINLGSFQYTYRYTSPNNYVINTGFCDEIAYQVRIVQFKMEIPNPIYGKFQLTSSNKNDGQVSIRHMIVSALKCYPSCKQSTGLKFNQCISCFYGLPTNNICPPCPDYQYYIKDQGCRNICDILVPLYTNGFCQSYPVYLFEDELARHEWIQWFVIYDQLHGDKSRPIINSDFYGIFKFNSGIYRYYKSLSTYYNSIQLVGFKITVVSINIIPLNCGIQFLINNTYFASIFRNVSGIQTHNTKIYLNKQDSSYLNYTSTTIYELITYVDLPKQPFLFSAIGNYTDNTAGWGLQTVQNDIKNQMVLNVLILMMKHLTLNIQLKTFQLKHTILNNTQNTHQSLKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLILCPSGLFYNAVSKSCSQCHSSCQTCFQGTVDSCLSCDTSLNRILKGLQCVCSPGYYELNSICTNCPDIEDILLSTCYKLCANNQQLWHTITCNSCDEGYHLIYGECQPICGDLQIYGYEQCEDNNTLLDDLCFNCQFQCPAHCLTCDESTTLPCPDVCGDGMITGMEECEDGNTIQYDGCFDCKFQCQLQCTKCIKGQCFECNTGGWYVDPTVQPWICKERCGDQLIVGQEQCEDGNQDDTDGCKNCKYFCRIGCSSCNYNTNKCLSCELPGFSPEKFYCKNYCGDGFVVKDPYGFYFEECDDGNNTNSDGCNNQCRFQCQPTSICLNCVNNRCEQCAPHYLLSDSKICIPICGDQIIVPYEQCEVPFILPYKGCQNCQAKCQSSCITCDNTGLGCNACKNGYNRIDNLCFSICGDKIVTEDEECDDGNLNFGDGCHQCSFSCPIFCSDCRKGVCYDCHEVSEDMVVQYVKVDFKILMVLVHLFVEINQLLQMNNVMMVIQYLRMDVMYVLNPCSCSYCFEGICFSCPDNHFLFKHQCFNLEQHANFKETTNFQFSDLSIIQTFKDNSPQTSRIHLKIDQFKKQVYNQVSQYIEIMDKDYSYINIQIIFQCSQNKKVQSQFSISNYENGSNNQENIWLNQCHQTVDHTITYSFGLQKVVLKDEIMLIQISEDELILSILFLRSPQKLLQITFVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNI3</accession>
    <name>A0A8S1JNI3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8043933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNI3"/>
    <dbReference type="OMA" id="HGYGHIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="39305" checksum="762258A0D2D76A2A" modified="2022-10-12" version="1">MGPCSILCCKKDDNQVVEKQIITEINHALTTEDRKVTEFQEKITSIIKIQAHYRGHSVRKKMNQQESKQFIKESSLSNRICIIEAIKEEDQQLIKQTEKNQEPVELPEIEMVDGSKYKGQWMKGQRWGLGRHQWADGSVYLGEWKQNFAWGRGKLMHADGDEYEGEWQRDQADGFGIYIHSNGARYEGQWKDDQQNGKGIEIWKDGSKYDGEYKEGKKHGYGHIFFADGSQYVGQFFENQIHGNGEYTWFDGKSYNGQWKNNKMDGYGTMTWADGKKYEGEYKNDQKHGFGTFYWGDGRFYSGQWLSGKQNGEGEYTTTTGENRRGYWEQGKRVKWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPA8</accession>
    <name>A0A8S1JPA8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040463</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPA8"/>
    <dbReference type="OMA" id="VEMFKNN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1027" mass="120306" checksum="82853D90C5F08015" modified="2022-10-12" version="1">MSLVKSFELALKQNDILSKVLPILKFINQTIPFPQKHSLINEPRIIRFLLQLHSLIPDTIDMILMSLLNIFKHSKEWIDGCFKAIEEYAQLPNQGNGKYVALEFLETVLIEISELDFDVDDKCYAFIIFLQNAENEKLVRAIMKKVGQIQQQLGHRMKSANQLNQLVEETVLKLYQEEVQKNSNEFHYQNVAIELQEKGSQARKGSKPKMSLMAMNILQSQSLSSKMPSKQQLNNDHINNNNSDDSIQGSEVNLDEILIQHKPRIDSEQSEYLADIDALQNLFAYITNPVATEPGLIFATDALANDIQQPCDDNQYKQHVMKPDKLTTLRSYKAMLFLTDYHNHQMIMQINSHTMQIAFRSISGFFQNSQSSGNLTHIIGLLQFYLKYELKETVYNFLYFQFHQQFLDYLYNPHYLDFLMRFMDPNYSKIPQDLRNYIWQFLNRQGYITLLVSKITNKEKEGIFNKRKDITKEAYQFLKHINDYKLQLIQDDDQAAITYSISSNIDQYLGPLIPGKTLIQNKQLLERKMLLSSHLRRGSDLQSLTRQINKVIIQENIDLSELNQQGKDIDRLQDVVQFYKEISTPAETPTLYNVYRQASEGMKNIQFEHRSQRSQATEPPLNIIQLQNSIRLPSLNIQPACIITELTTSKNTQRKQKSMTFSENNLGQTINKRSDQPQYMSLVGTSDNISTYRQINLMQTPTSQAKLFVYYPNQDNSVVDTIMKEEYAKYETQNDEITSLRNSNLLKIIIQSMQNFEYHYAFDELLDDQYILFEKLLNHYSLTQTALFQSSYEENSSNNCQIILIDLIQRINKKPMNEKLSKLYFIYLPRICRNLTNMHKMQNEKISKEEFFSNQNHIGSIRIFSTKIIDLILRNFKQRNHRIYLKLNDTSSFNLNEQFHQLPNPFYQQIFYSIMKQLCLFAQEKQVQAILFKANLLEGISQAFHKNVVNKVIVRQGNVEAYLLHLALLSQLIILVYELRDDLQSYKTSLLRLQCFQNLKEVIEMFKNNKLIQLQALDIQKQLQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPY1</accession>
    <name>A0A8S1JPY1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0060273</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPY1"/>
    <dbReference type="OMA" id="FINKPKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004485">
        <property type="term" value="F:methylcrotonoyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000028">
        <property type="entry name" value="Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050856">
        <property type="entry name" value="Biotin_carboxylase_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866">
        <property type="entry name" value="CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866:SF33">
        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE SUBUNIT ALPHA, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0092">Biotin</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="12">
        <location>
            <begin position="3"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="11">
        <location>
            <begin position="122"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="10">
        <location>
            <begin position="588"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8044191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="665" mass="75048" checksum="CDBD6DC2CE893237" modified="2022-10-12" version="1">MSYINKLLVANRGEIACRVMRTAKKMGIKTVAVYSDIDKNTLFVEMADEAYNIGPPQALQSYLRSDKIIDVALSTKSKAIHPGFGFLSENAQFSEDCQKNDIIFVGPGADAIRKMGSKSESKIIMTNAQVPVVPGYHGENQDPNFLLQEAEKIGFPVLIKAVMGGGGKGMRIVRQKSEFLEALEGAKREALKSFKDERVLVEKYIEKPRHIEVQIFGDKHNNYVHLFERDCSIQRRHQKVIEEAPSALDENIRYDIGEKAKEAARAVKYSNAGTVEFIFDLDSSKFYFMEMNTRLQVEHPITEMITGVDLVEWQLRVASGEHLPLTQEQIKRKGHSIEARIYSESPNNNFLPGSGKLDHYQEPQSSQSVRIETGVREGDTISIFYDPMIAKLVVYGENRQLAIQTLLNALQNYQIHGLPNNITFLKTVLQHPEYVNQQYDTSFIAKNQDNLLKLKDNYNPIDIALAIVGRHFLNSVQLPKTLLNFRNGAQINNKLTLNIQSASYAHQKEIKINHNINGNNHELEIEGSKIIVQKVTKSNEHNNLLIFETNQGIFIRTIRNGQNLLIFDAQGDPITITVSSDEVKKAKQEQHGHGNNKEIVAPMPCTLTKVNVKVGQKVKRGDILIIMEAMKMEHTIKAAIDGEVKEIRYKEGQFIEPGAMIVKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQN3</accession>
    <name>A0A8S1JQN3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080432</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQN3"/>
    <dbReference type="OMA" id="QQYFSKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="459" mass="55473" checksum="9694227824BD8B56" modified="2022-10-12" version="1">MYSISEFSEFQSKIVHFQSKLTDNDKNNFQFCYNIYKKLKQQIKQAQQYFSKFNFLLEFYDLLEEFQRDKQIQEYMKCYYYLLPIIFNNQIYSGILESTQFQEQEVQKLLNLTNKLKSLELTEQIDKDFKNPEELKLKSSLSRHSISYHNKQQICKIPYQLCYQYFYSFHLKFQQNGLYICYQNYNKLKMALNKIKELFIQERKLYKLMELNVKNLKAQKIPKLKLIDLNVNSKQIQQNFNDKINLLQFDNFEIQYLQQFSVYAQSIINHNFEKKDHFGLSKISVSHNNQLQITSRGDDYQIQLELKDIMKNEIIYQLKQNNRGLIDIQFSENSKYFYLLQSFIFKLYDIVSKSELLNLHIDLEYQEERFLNAQLYSNDTKLILLSDLQIKLFSFNGFQFLLEQIFNIYNISLINKIQIIESENSKDKIIEIKGKLSTTNSIYLQINDDNYKLIQFYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWY4</accession>
    <name>A0A8S1JWY4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0090166</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWY4"/>
    <dbReference type="OMA" id="SYVCAWN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097014">
        <property type="term" value="C:ciliary plasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF26">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 2 INTERMEDIATE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="404"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8045140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="546" mass="62987" checksum="8B87292C0B90D026" modified="2022-10-12" version="1">MKEGIFDDFNDKEQIFEFTKGKQTYIRDMGTVTDYKVKNQGTTTKKECQDFEQQIDQNELQQTKGKLYIMQFDQNKLSQFLKRVLPTVIKELDESLNLKKYHSYKPISEVQKKETELVYILTNAQNKSIELEKKLEITDIQWNSNGLMIAASYGCLEHMGQCSHTSYINCWNIFKRDFKPDKPTTVIETNTCNMCMQFHPTKPNILAVGSFNGELYLYDISTNSELAHSTIDEYYHRESITAVLWLQDESIMTLGCEGKVLIWNPERGNAEFSLQNPLKGCYLVRKKESQTESVGGVCISQSNEDPFSFIVGSEGGSVLRAQITPINYTMTKQAYLDSRDKGLIWKEETILFMQNLNQKCLPEIKLHVENYCKERKINNIFIQQLVNSKPDIRKMYANPINYAYEAHYGTVYCINFSPFVKSAFITASMDGQIRLYLQNTARCLAVYEYIQTYILSAQFSPTRPCVFAACDSLGQILIFDLTQDTQQQVVTLKNDGQSASCLRFNQRQQDLLACSYQGCEVRIYQLNDELTQPKDNELKELQQLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYG6</accession>
    <name>A0A8S1JYG6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0120112</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="5">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JYG6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000238">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001058">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="13"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8047802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="491" mass="56993" checksum="197546A6D6D16711" modified="2022-10-12" version="1">MSEIVDQNILKKYEILTKIGKGAYGIVWKAKDIKSQKIVALKKVFDAFNNPTDAQRTYREVCLLKKLNHPNIVSLIDTYPAENQNDLYMVFEYIETDLHVAIRAKLLQPPHRRYITYQIFKALKYIHSSGMIHRDLKPANILLNSECQIKLADFGLARIVSTWEDDILTDYVATRWFRAPEILLGSKFYSTGVDMWAMGCMMCEMIMGKALFSGTSTINQIEKIIDVLGLPTQEDIQSFGGQKQLFEKFSRNYKFNLKSILNCGNDEFDLITNLLAYNPNKRLNAIEALKHPYFREFHNVEEEITFKGQIKLELRDDKLFPIQIYRDLLYKQNKNSNRMINLINKQKKLIQIIEKKEIESLKKAKSQQQINQQSTKSAFLQNNSTTNFDKSINKTVAIPLQNNYSKQQTNFNNLKQQQLQNRLKQICLSSPDKIENTIQSKSPKPQMLIPQRQFIKTNLKPITFNNLIDTSLSPQSLSKQQIINSLTQMQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0N6</accession>
    <name>A0A8S1K0N6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0N6"/>
    <dbReference type="OMA" id="IIERMSY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="312" mass="36143" checksum="4B617362D9312A34" modified="2022-10-12" version="1">MQHKQIKATTSQNIRQKLTMKDSLTNINQIYSQPHSSKGAETRKKENVQDCNTMKKKLFNMLGQNYNSNKAFSISPTNSLKQNYFVSTSTQQKSKQLIQKQNHTLDCHTSIAQNPQGNLVRNVQSQQSLNQFEKYTSFLENQNFENQNPSVNPSKKVGIVKPNSTQSRNHKTDRSSLIALFEKLHQKDSSNIHRKQTSQNSNYINNVQNQQLPECFYTELKLRQKIKGSLSNNSSGQLCSHLNPNSSSTQIQQNQQNTIEDNLQQIIERMSYILKNYQSKLKQVDFEKEVLIQEIQYWKSKYQKCQQKSSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8L0</accession>
    <name>A0A8S1K8L0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8L0"/>
    <dbReference type="OMA" id="YANFPHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035713724" evidence="1">
        <location>
            <begin position="16"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="477" mass="53233" checksum="FDB76B2A716F15CF" modified="2022-10-12" version="1">MNILLISSLIYLTLQQCTEIGVAQCNGQLRSGLLPTYLFEKAFSVGLPSKFSTPVQIIQNGWNLTAYKFISEALIDNQTYTGQQGVGEGGQITVSNPTILIEVGFLFQNGTIQNGQGAAILESVSSASYTFGITTKTNPYKYSFQYVNSTVNFNNAVFKAVYANFPHEIGQIQEILQAINTNPLIQTALTQYYASSSLSGLKSTLTRELNGFEIIKGQTSTKTYKNGAQNFNYQVIVNNVQIQYAPVSYAISAEMNIQQLQTQYTCNQPINTVCTATSNTNIEQISMDEIINTFNYAFQQNYFKTSLNPQYWKVPYFSWSTGGLQNIMNNVYTSYAYASQIGGTCIATSANYVSYNTKYFTVAMTFTCNLTVNPNPILSFTVEFDDIQVELDFSKIKEQAAFKIKSANPSNITLQPQGNYTIDNQDLIQWYIQEAIDKSFVGQNFFAGIIDSQRNIESEEITYQILNGIITITNNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KA93</accession>
    <name>A0A8S1KA93_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">alpha-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0170126</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.24" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KA93"/>
    <dbReference type="OMA" id="FIWRPSK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10810">
        <property type="entry name" value="GH38N_AMII_LAM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000002">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000011">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF3">
        <property type="entry name" value="LYSOSOMAL ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="11">
        <location>
            <begin position="357"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8050575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="958" mass="113663" checksum="2A872A6F7513C36E" modified="2022-10-12" version="1">MKQILFISFYVFNVLCNLRFRDKEQLERHHSHTEVVDEFLTVHVIPHSHDDVGWLKTVEQYFYGLRNDYQWANVGYVIDSYIEELQRDENRKFIQVEIKFFKMWWDQQNETTRNITKQLVENRQLQFISGGWCMNDEATTYYEDIIDQMSLGHKFLKDTFNYIPNIGWQIDPFGHQNSQAALSYQMGFDAWFFARIDFEDKETRLDNKNMEIIMLPPAEGVQYPIFTHIQYYHYEAPPTFNFDILRRSDAIVDNQKSESYNIKERSDQLVSYFKSQSLHYKNDHLIHTFGSDFCWSNSKVFYQNLDKLIKYINMNPEYNMKLQYSTPEEYIAEINKQNTIYETKSDDFFPYSDEPHAFWTGYFTSRVAIKGYVKRLGRQAQTYRKFIALLYQQELIQDNYQNITDTIYELDNALAICQHHDAVTGTEKQHVNDDYINILYKGEQHIQKLTRKFLSQLLYNTTTIDYEQCEYNITAQQCSKTYNALKANKTVILTIIDGKVKQENNTDIIRIKVPNLKLLIKDQNNQVLYGDIDCTNGFQDCDLYFKYQIPLNNVIQYFTIQPVLYNESASEIQLTVNPFPYNEDTLITLNSTKSFKLKYAYYQSYQQKDQADGAYIFRPSNNSKIMYGNISYTAIGYGRIMSIFRIVRTYTKSVVKNFYHQKNTFDIQTFIDQIPISDQIGKNIIMVIEVPNFNSDNTFYTDSNGLQFQKRIINYRPQYNYSLHENVSGNYYPITSGIYIQDGQECVGLLNDRSQGGSSLQNGQIEIMIQRRLLQDDSRGVGEALKETDEKGNGLVQNIQHKLTFFNIEQNPNQIRKLQYYLDHQPLVFFNFDQQLPLIPQKLNWFLPFDGFNIDPLLIYGENLLKFNYILWNEDEILLRIQNMQEKFSMKIDKMGFEGQAIRTTLTGNQKWSDWKNKSLKWNYNKQENTLDQDLDDVLYPLQIQTYLIRNSELFYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KBW6</accession>
    <name>A0A8S1KBW6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KBW6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="95" mass="11331" checksum="94AA5C660DC1AD4D" modified="2022-10-12" version="1">MDDEEKLTATEFINWANSIFKLEKDKCLQFQPNNDQSLTSTLKNDTSQQSSSQINRFTYSTRQLTRMMNENYHLYSQFKYPNITNKMDSFLIIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJN9</accession>
    <name>A0A8S1KJN9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJN9"/>
    <dbReference type="OMA" id="ICSDSCM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="598" mass="70550" checksum="4EB1C368F46DE4C2" modified="2022-10-12" version="1">MIKRKISKFTPFDRVLDGLEQEELIDEDVNMKIIKVYNRKKNNKMFLLERLISNEKEHLKYIDEIHKYEIINADGLLCIDKWTYDMLIDQNLNKLYKFILVFDTYSQNLNQYLENIQSKKIQLLKEDIVVFFRAIILSINYLQQQQIISFNPVTMSCILICSDSCMKFNCIPILLTQHALEKAKKESLLYQAPEQVKDIKNINHIYSQAVYSLGLIFIQLFLLKHIDQLRKYEDIKQSIKEIQIKYGDTYKSYLESMVEQDPHKRIKLQELCQILSIQEPQKQIKDNIKITFSETFEYYRKQYSILNVPEPRSIAYGIPWSRNIIQSNLFNMKNYFLELLDLSQSQQISEQAVTIIFQQFRVFVIGGMNTHLVYELNDDIPALKEVTSIPQSNANRWGFGYVIQNNQLLYICSGYSDQLLTANTFCYNIQTQKWTKLSDCQKPGIGCTLVCYNNLSIFKYGGTEINNKPLNCVEEFKNNEWQLINFKKPHFILPNFSFAQQVNPTQIFIVGGYFDKEHNTKVIVMNIDTQAHLIDSRKDYTYVEFVVNEFLDYGKYGPVEAMQISNNQLFLLQKKNEKHQYLTVQDQSVFVELRKIHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQV5</accession>
    <name>A0A8S1KQV5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0230263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQV5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="194" mass="22513" checksum="65259AEEBA5C9869" modified="2022-10-12" version="1">MSDQLNFNLKLFRERITDKYQRNSSNTKIGQDNQLSSQRKSFKKNAPTLDLLSSKLQNYGDNKFKTDSRVCSPKHLSSPVTINGMQQKIKSITTSPQRFINNHFGHPLKSPKSTITQFQGFHQSPRAYTDRNEYQEIKRYNSNNDITEMIPLHDLVNMRNKLENYDINAAHISPTYLSEIVKLAHVINTQLQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRY6</accession>
    <name>A0A8S1KRY6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0240102</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRY6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="2"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="181"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8056022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="32058" checksum="0D698B3B3366278E" modified="2022-10-12" version="1">MGDYVKALFETQVIGAFRVALTMPLEHVLDRIKTYKQSKQSITYIQSYTDIKGARGMIGLYDGFSPSFLRNMVKQYYRWPMIIFIPSILNEHIHNQSINKIITGASIGLFESCIITPFERLKTLKMTSMSTGFGYYKYITFESIYVGFRIQTTRQVVSWTNYLYWDHKVRYALKAEPGQPLSLVNSLIAASLSSILNIMAVHPFDTIKTLVQMEGNQNFKNLSLYEGFKIVYKNYGISGLYAGWQARIIAYFCQALLTTPTIDYLERNYGISKNKKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSX3</accession>
    <name>A0A8S1KSX3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0250202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSX3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="202"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="393" mass="46155" checksum="3E43DBC8C079F4BB" modified="2022-10-12" version="1">MLNTGIQDQDSLQIKHIDLLKFGNLNKKYINLYIQQEQQVNTYLLYSTSLFKIPIINELFPQIAKFYINKMQQVINHVLKYYILYQQCFRFKLQDQFMIIPFFIFLILQNINMKRCELKQQIMLEVLFLDQQLPFNDILIKFNNNFTSQYELQKQHSMFSFINNKYSKFMSWGEPAEVNIQAQDGGNDAPQNNWGNTEQTWGNNDGQQQDVQQGQEDQQNERKGKKGKKREASPPPVKRVRPNPIVIVVDEDEDSETQNIIINECPKLQNKNKGLIQNENKQICYEGNLKIEDCVVNTRFISFDLEQQVKIDCIGEYTIENAKDLLKTLNDQVIIGIVEGLDEENQKVINKLADNLLDKQQSIKFKDLSLFSIKQLYKIENKSRLAFIKQITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYU8</accession>
    <name>A0A8S1KYU8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0260244</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYU8"/>
    <dbReference type="OMA" id="ENYMLKT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="294"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="48"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="314"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8057836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="348" mass="41191" checksum="23EF258625CDA289" modified="2022-10-12" version="1">MNEIFKLVCFDGAQQKKWSQCKDLDSQLQFIRDAIPQKINEATKNYDYEALEKQVQKYEHEIRKHIRMEQQIKLYAESLQQKLEEQEGINECLNNTKQLVNSLKKENQQLIEQVKRLQSENYMLKTKLIDVNDRINSQQTSPNYDKTKNQDSYHIKNSQRYQTIDFVGPKQKLNQRNMDDYSKQYAEVKTQISNTIDSSILKMLQNRRDSLKLNDAYKSSRDSSTIEKNDSNKIKSCINQQRAASQQQKTQHKQIIDSSIQQVKTTTENVKFDLKNQSDNLRAFLNTQSFLCNKKSKTNSRPDNRYNKSHSEHQNANTNQSEIQKSNKIQKEYLQFSQNYQKYLDNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYZ4</accession>
    <name>A0A8S1KYZ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0300282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYZ4"/>
    <dbReference type="OMA" id="QNHFNEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="344" mass="39852" checksum="F0D56B3338664D84" modified="2022-10-12" version="1">MDPQTIQVIGFNELPQIDPNQLTQLAAETQQLFTYGTKAWLQLFQAITNLRCINKYVPQYVNQLFEMFGQQVMISIDDSKPKIQRNVVTLINEVFSLGRSVQICPQIFQVFVPKLILKSQNSEHKQIKEECLVAVSNYILNCSQYEAGLFAVIQLTGQKKSTVPLQKFCLQVMAKQIQTIGSFLQSFSVNTLTLLLQALSSFLNSQGGEIQTYAFQICMYLHQLVGDQNFKNWMSVSLDPQSTQIMVNKIQEVLRKKSKAKQHTTMTDMKQRMTFGIYENQEQVENQNHFKEFVKDKQKANNFLDLNQVQPLQPSVYYNENLNYQQQPQHSMQNLSQQQFIQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0Y3</accession>
    <name>A0A8S1L0Y3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD_REPEATS_REGION domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310188</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="EDPNCAS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052993">
        <property type="entry name" value="CFA-57"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="57038" checksum="938103FE35A61A3B" modified="2022-10-12" version="1" fragment="single">MDDKKRRNEDRRLKLDVIQSYGSLCSAKFKDSISIIEDEQRRLLYPVGKFIAQKFIDKPDTQFIRLSDNLEIVSCMTVAPNKRTLAVAEKMKNETVPQLVIYNLKAHPSKMEAEKKPYKYLDTKSSTFISMAFSHTDSRFLLCLTGPPDYLLIFVDLIRMKHLALSAIGQELTRVSISPKDNHMVAVSGKNYFKILRVQENSFISLTDQIKRLAPIQTFTDHAWFEENKVILANDKAEIFIIHDNDVRQYINNAFNDIRGPGISSLIAFSKGFLIGSDSGHFALWIRGDDHDKQKTEDGIEVIMDLQFLKKWRCERKSQVVSMDITKQEDLVAVAFSNNDICTIEMGQVLPNSTEVLESYKNNEKFLKREVKFDFLFNGFHNAPITHLDVCLQRPLIVTASKYDSTIRIWNYAYPRCELARKFYLGDDGNQQGLGTKENQSTTQDISPLCSVGFHPTGYYLAAGFQDKLRIFHVLHSELRAYKEMAVKCCTQVKFSX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L169</accession>
    <name>A0A8S1L169_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0320072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L169"/>
    <dbReference type="OMA" id="QCITASI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052145">
        <property type="entry name" value="Mediator/Homeobox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330">
        <property type="entry name" value="HOMEOBOX PROTEIN BARH-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330:SF19">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="465"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8061670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="64538" checksum="AFE51FF9F98531C2" modified="2022-10-12" version="1">MEFEKQIYQTHSFFLSFSLVRFFVKLGSFIFPILSILGFCALLMLHINQQMTGLKLQETDLIDFDNETVYNFNITNSNNENLNQNFKYALSQSLLQLQDEFLNKYNFTQKTIMINGDKYQSGLNSILQYTTNIESFFLIDLIHHMNGSTIQIVDMQNNISYIWEKSYFEEYYANTFSERIYNTVLRICKCIIGLFFQCITASIYFRMLFIYMPVFSLIIIGLIFCRNHQELQILARSYPWINHYFEILTRNNKLKNQIIDSFLQTLYMFLLIFGLTWAQVNSLLFKKQHSFYLIDKITQHAFSFEYFSLHFLRTRSSLYFVPKYCFIIRFFLYYYMQCTLFGHYQLVQYISLFGQLGVFCYFINKFEIPALNWSNRSPYTPTINRPRAYYVPLFPMSWVNDTPQLWSMFYPLYGRRYFQIQNLALVDQNFPLLNQILEQEIQQGLEIQQDQEVQIDIIQQIQIPQDNHQNQIIDQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLNQIQQVQVVHQNQEIQHLNQEQNFINQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L430</accession>
    <name>A0A8S1L430_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0300082</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="QHACTCC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="21"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8060333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="186" mass="21043" checksum="88E2294DDB4E5E4C" modified="2022-10-12" version="1">MISFDDILENKIKTGRYQFKTLGIVGLVEFCDGIEYAYMSILVAIIQKEWELNQQQIASLGSSFLLGMVIGNCICAFLTDIIGRKITFTIFTGLSVFLIYFTSICTSYNQMIMLRLMFGIVFGTSYPLGYVFISEVTEPRYRGRFGYSMGVLFVIGKXQFIFKQQIHSCPNKIIQHACTCCCLSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5V6</accession>
    <name>A0A8S1L5V6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8060933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5V6"/>
    <dbReference type="OMA" id="HHIYTYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="760" mass="92834" checksum="9286404537D30877" modified="2022-10-12" version="1">MLNNVLLQFDKLNNYYHKKISKYELINSNKILNHMINNVLLQFDMLNNYYHKRISKCLLINSNNYLNHMLNNGLLQFDKLNNYYHKKISKYELINSNKILNHMINNVLLQFDKLNNYYHKKISKYELINSNKILNHMINNVLLQFDKLNNYYHKKISKYELINSNKILNHMINNVLLQFDKLNNYYHKKISKYELINSNKILNHMINNVLLQFDKLNNYYHKKISKYELINSNKILNHMINNVLLQFDKLNNYYHKKISKYELINSNKILNHMLNNVLLQFDKLNNYYHKKISKYELINSNKILNHMLNNVLLQFDKLNNYYHKKISKYELINSNKILNHMINNVLLQFDKLNNYYHKKISKYELINSNKILNHMINNVLLQFDKLNNYYHKKISKYELINSNKILNHMINNVLLQFDMLNNYYHKRISKCLLINSNNYLNHMLNNGLLQFDKLNNYYHKKISKYELINSNKILNHMINNVLLQFDKLNNYYHKKISKYELINSNKILNHMINNVLLQFDKLNNYYHKKISKYELINSNNYLNHMLNNVLLQFDKLNNYYHKKISKYQLINSNNFLNHKKYIKLFSQNKQHSQDRNLIDMYQVIHLNTYSMHMTNNDYYIFNKLNSFHYTYQHKCYLLNLYIKDNFVHILISFQIQVDHMIRIYCLRLCHTLCNNQNFRSILNSLHHNLNINCQIYTTLINKYRKLFHWNMFHIQQDNLNNLQVHHHIIKWNINLILKQSELKCLKTKSSQFLLIFLKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LAE3</accession>
    <name>A0A8S1LAE3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0340261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LAE3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="348" mass="40950" checksum="17347C0B49BEF320" modified="2022-10-12" version="1">MSLIYKNHLVLPTFKHSETITTHKSSSLGPIKRFQSVTPIRQGNQSKILPLNITSLPGNETSINAESTITSRQKHLPMLTPRIQRNDEQNLHDYLDHLHSIRKSKQPTKQAVMKKIRTETEIMKLESQTLIQRFKKVKEQQELKKQYSILQTIKNPYAQQYINFSKQIFNLIHNLHQLKEYLNNSRKMFQVVKNLIGMKKNLKKILLKQSADDLIDFQDIAKMCCIREIQYINGEQRMIDKFDLTLMDDLTYMCEAIENLLLQNKLNAIKRMSDNILIEEHEISKLREQLGMREEEQKLSVSTLPMQRFKPHVELDNIEQRLGQLSQIPKQYRQTSQVLCEIVDKLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBM9</accession>
    <name>A0A8S1LBM9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8064977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LBM9"/>
    <dbReference type="OMA" id="EIQLTHS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="14791" checksum="DDCB11B33308CB3D" modified="2022-10-12" version="1">MGSICKQGQKSSNEKEIQSEIQLTHSKEQYEARHNSVSNQYNCQSTRQLTFRNDFASKFISLSQSSDSEPNQDNDPGDKRSFFSNFQTFQAMPSQKSSKLVSPQFTFFADENSIENQFQKKLQTRQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIC4</accession>
    <name>A0A8S1LIC4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0410251</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIC4"/>
    <dbReference type="OMA" id="QKIHQPL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF89">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="2">
        <location>
            <begin position="42"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8067958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="339" mass="40924" checksum="2B065FE3BA6196B7" modified="2022-10-12" version="1">MQTSPICPQPEAQYLDNYENFEEVTKTFKCLQKIHQPLEEEATYFLIRAPTKDNVHRAIKYGIWTSSSRNNQKLNDATRPVYLLFNVTQTSHFIGLAKIVSNFRENMHFMYWAEENKWFGSFQIEWVFVRDLPYSELSSIQQSGGKCIHELIDCTQIENGHLIYDAFKNQQQKSCMLKAFKELDNSEKRKRNERDSNPNFPIQFQEYINVFEQMPFTFSVASYQRRKQQQIQNQCYQQYQYYYQSPWYGVAQPTFWNQQQQLQQQPIQQYQQQQQQQQQQQQQQQQQQQQQSLNNPGFYQTSNYNRPQGQYHQNNKYQQKKQLNNKNNIQQSPKKQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKR2</accession>
    <name>A0A8S1LKR2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0400094</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LKR2"/>
    <dbReference type="OMA" id="VYRESGW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000180">
        <property type="entry name" value="Mitochondrial carrier protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF12">
        <property type="entry name" value="MITOCHONDRIAL ORNITHINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="13"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="103"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="199"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8067065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="33820" checksum="5413D29D35C2561B" modified="2022-10-12" version="1">MSDFIQWTYDNRIQISIDLAAGSISGIANCISSHPLDTVKVRMQMSNDGVHSTLKKIVQNEGTRGFYKGMSFPILSIPITNAIVFSVYEFWKSFFIGNSNKQLTYFQTAICGSIAGSSAAFFSCPIELTKCKLQMQSTDKIYKNPIDCISQIYQKEGFKSLFRGMCATQQREILGYSTQFAVYELIKDFLCNLSQKAEPSTANLLISGGLAGVSCWTIGYPQDTIKTILQCQQPSDQTLYKVRFYDGGFLDCLRKKIISEGIGSIWKGYSVCILRSFYANAIGFYAYELAKENFTSLYQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LL02</accession>
    <name>A0A8S1LL02_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0430041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8068868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LL02"/>
    <dbReference type="OMA" id="WDGCENG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8068868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="249" mass="28835" checksum="83B90B1FBF15A25F" modified="2022-10-12" version="1">MKQQLVIQGVATGSSTKVTLKPKPVQQKPKYQAPPPKQSIKKQTKVNVPQQQQSKQQQQQQKPPETKVVQLTESILSDKSEPDQQQFKLQQEPIKQNEFAQIHVPVYGEVTVKYSHYNEKFPIKDGQLLAKAIDEKYCLSFVFQGQYKLILKSEDKKKIFEFSRGGWDGCENGKTYYVEVEEDPIQEEWDRKNSKPYQAPKEDTCPLKNKKLELITSELKAMSIDEIKDKGSKFKELIEARDLEDILFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LLF8</accession>
    <name>A0A8S1LLF8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0420224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LLF8"/>
    <dbReference type="OMA" id="IEREFHP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051493">
        <property type="term" value="P:regulation of cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000180">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010441">
        <property type="entry name" value="CH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052111">
        <property type="entry name" value="Spermatogenesis_Ciliary_MAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12509">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12509:SF8">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06294">
        <property type="entry name" value="CH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="1">
        <location>
            <begin position="7"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8068567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="833" mass="97981" checksum="469638F0DE32C051" modified="2022-10-12" version="1">MIEPPQGPIPREVAKWLDSLDLAYQVRNLRRDLANGFIIAEILSRYYPKEVNIYTVYNEQNLDKKRDNWEQISKLLKKKELQVPQEEYEPIYYQAPDAAQALLFKLFEFLTKKKIGTIYPDKDNRDMKKQVKQQATLQRTKPMTVQPTYARPTANTIIKDREITRIVDENERKMLTKQAIEEHVEKVRNDKKDDNIIEYLILKRKKQIEEEIKKQEEELLKKQMKFNKKLVNLDQSISQTGQDVKEIQINCYHSSTSKKAKEQQGQLLETKGVIDFLHEACASLFKKDPIKKDIQDLKLEPKESQQNNGFQVSNQNVQYTAVIQQLFLRCDEVQKKSLKLLLTQFIDVHSQLILEQVQSNFIDFKRFFQIFLRPIQILKPTSKTYQFILELIRFIGSKLVEIDPNDTQMMFESILLDQLLQIALRNPKKREDIVQLFFVFTPINCVNRTQLLKSIKSKLSTDLNAFTLILSILIQSDYPSQDEELYRFFLNYAFNSLDSPSPLTRANGLRIINQISSWDYLPVLQKISRIQKLMNDSWWEVRAQGMSICADLLLQIAQNSNKSMDEQEFTIDFEQSKQLLLDLIYQVFVVSTTPNVIRIGLIYLAPVLQMYSELCPRYLEILLSVDSEIRISVLNTQETLIQQVVNGCNSFRYKITGAPIVWNSVGIAQAMDVYVKEKDIQFFKHEHLEIIYGCLHKKLLESDGEIWLKIFENTKRLLFLALSNEDLCSIAQVILQKFFMFQTIQQQVLDNSNEIFQRMLQLIYHPDVHLKCKENLLEFFTFLQQQGFQNYCYNIIKQFSEKNKNMFLSSNLVEFMNGLAKEKRREIMGDENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQ05</accession>
    <name>A0A8S1LQ05_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0430081</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8068909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQ05"/>
    <dbReference type="OMA" id="NYRSTDC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc-finger" evidence="8">
        <location>
            <begin position="129"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8068909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="37973" checksum="39E939D9C2E5C93B" modified="2022-10-12" version="1">MHTQNNFMYNTQGYMYPQQYMYPHPHQHQPPNYVFPQSIQPPQFYYMNPQNPAMYQYNIGQQQQQQQQQRIKCNQPKTKEQELKQQKQQVVKQQSNQNIQQQIEMKKTNLKILTEQQIKDQVGSITKQGFCHYCNKNGKMCYYCFSNYRSTDCRHGFCFDCLMFKFKINPIKILLRPDWSCPIKCKQCNCSRCTNLNQMDSDQLISNDSVESCNKYAHIIENTKKIKKQYQKKKNIIREMFQVKFQQLDPKKKKQQQSMKKTLLLNKIKKQMQFSQECILKLKQKHIQNDEQILHQVVRYNFGSLIDLFKEYKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQZ2</accession>
    <name>A0A8S1LQZ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0450251</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="137"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="700"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1075"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="700"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1075"/>
            <end position="1087"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8070450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1145" mass="134597" checksum="373EF7130466946F" modified="2022-10-12" version="1">MSQQVYWPVYLENDICDKFWNRGDCVLDGEILVTQQLNKTDRKNQVIKERTYILTEKGILCYQKQSNIKWVLLTYQMSVKLIDLKIQNKDQDLVKVIRVKRNQQVYSYFWNADFQQTFTFFKRLTAFCICQNFEQLYTIQDLMGKGGYSKVYKLCPLNKQSFQKQYYAGKVYNKNELAQKKNLNCLAQLVKAECEILKQVNSPFILTLYEIIQLDEQLILITELLRSGSLYKLLREKVRIQESEASGIILRVALGLQAIHSIGYVHRDIKLENILIDKDQIKIIDFGFAEQINREVLTSGQGTVGYMAPEIFTHKPYTELGDVFSLGVVYYLLLTGKPPFKGLNQDNIIRANQKCDIDFSEYCFVNVSQKTIHLVKGMLQKNPKQRSSLNEVLQNLKSQNLFLDIVYRSISIEGSQLTKHGGMRSLYLQSQCSQDSPRMQQKQSKLLNITQTLEKFAVKTSSFRPSLQNIDNYTFDSDDLEDNTLPQFVLENPICLNCQTWQTKNKYSKALYSRYQSNNSNFQLRSSTIIKYQDQVCYEENEDFYTLQNSKKKHKQLLEYYRYHTDIPRFFIKKITSLLETHHEKKREIKYNQIKFQLGMLVIQPEEDQHFSFKALQGLTELQTSRQLQQLLKLIQSNKYDTKNIAAIIKEQKQQMAQLNKKEQLKKILNIEIQQTITTKRPTNHTQPTLLTQPTQYTSFTQHTQHTQPSQHNLGSPKSHKNKMIGSEIYGNVVKKEFYKXFYQKTIHLVKGMLQKNPKQRSSLNEVLQNLKSQNLFLDIVYRSISIEGSQLTKHGGMRSLYLQSQCSQDSPRMQQKQSKLLNITQTLEKFAVKTSSFRPSLQNIDNYTFDSDDLEDNTLPQFVLENPICLNCQTWQTKNKYSKALYSRYQSNNSNFQLRSSTIIKYQDQVCYEENEDFYTLQNSKKKHKQLLEYYRYHTDIPRFFIKKITSLLETHHEKKREIKYNQIKFQLGMLVIQPEEDQHFSFKALQGLTELQTSRQLQQLLKLIQSNKYDTKNIAAIIKEQKQQMAQLNKKEQLKKILNIEIQQTITTKRPTNHTQPTLLTQPTQYTSFTQHTQHTQPSQHNLGSPKSHKNKMIGSEIYGNVVKKEFYKRWSDVLSHRQLEFHKNKMKSSLLKKKISLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LRQ6</accession>
    <name>A0A8S1LRQ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alcohol dehydrogenase-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0450167</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LRQ6"/>
    <dbReference type="OMA" id="WMHNYYT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05282">
        <property type="entry name" value="ETR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051034">
        <property type="entry name" value="Mito_Enoyl-ACP_Reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981:SF2">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="3">
        <location>
            <begin position="26"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8070317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="305" mass="34061" checksum="FDC0F463F2C77819" modified="2022-10-12" version="1">MKAFVISKYGTKPLWQQVLKPSLLSHQVLIKVRYAPVNPADMYYSLGIYGIKKALPTQLGMEGVGEVVEGKHKGKLVAFLPARSVGSWAEYVAANESDIFEVGNEIQGSMAIINPFTVLGFQERAKQFKSILFSSARSSTAIQGIKLFKELGKDVWAIAKENKDYVNTLVDDENLLQNIQKNIKDKTVFFDSTSGDKAGTILTSLPANSVLVNYGSSTATFIGKVNPNQLIFQGKSIEGFWMHNYYTSLTRQQKEVAFNYINSKLDTIFKSDVYQIVKPQDISGEELYKLQSQTRGQGKIILEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSM6</accession>
    <name>A0A8S1LSM6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0470191</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSM6"/>
    <dbReference type="OMA" id="IWHSYER"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="64"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8071458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="668" mass="74947" checksum="F68ABAB6A2321AE4" modified="2022-10-12" version="1">MGCGVSNAHQFMRYSQEKITENEPGSLEQNQGQTTVRRNVKYANQLLMIPQQGITTLQEMINNSVAKFGNQPCMGKYEGDNFKFMSYNQVNEEAIHLGSGISNLNLVKEVQEYQHYKLKLIGVFAKNRREWMILDWANIIYGYTMVPFYDTLGPESIPFILDQTNIETMLISGDATKSLIQCKEKYKLKNLVLLDPISDQQTSELKNKGYQLFKYEEIVENGKKQIVQPAQVLPSTIFTLCYTSGTTGNPKGAILSHGNFISAIASTQATDAGINQNDVHLSYLPLPHVMERLIILTMLFTGACIGFYRGDPNLLKEDIQKLRPTVFASVPRLYNKFYDGIKAKINEVTGLKKSFAERAIRVKLENLRTEAKYTSGLYDKAFQGVRDLFGGRCRLMITGSAPIQQEVIDFLKIAACCPILEGYGQTESTALSFSTGVWDPKSAHLGGPAANTEFKLVDVPDMNYTSLDVVNGVKTPRGEICLKGYGVFQGYYKDPEKTAEAIDANGWLHTGDIGLITENGGVKIIDRKKNIFKLQQGEYIAPEKIEAIYNRVQGVTESFIYGDSLQSQIVAIIVPSKEFVEKYAAEKQIQGDFEQLCKNPEIIGLFQKNVNDYGRANKLNSLEIAKLIHLEPQPLQTFGCLTSTFKLQRHIAKQVFSKQIEQLYKSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXI9</accession>
    <name>A0A8S1LXI9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">COMM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0450139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXI9"/>
    <dbReference type="OMA" id="FPEQFED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047155">
        <property type="entry name" value="COMMD4/6/7/8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231:SF4">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 4-8 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21672">
        <property type="entry name" value="COMM_HN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="23118" checksum="793859391F26285B" modified="2022-10-12" version="1">MRFLFCGDSNCPEWFLAESALLTKIASVKMKLVTQYILQNIVEGIDTSAKIFKMLEGSGFNQQEINSTIACVSYIVENSAKYDVSEQILIKELIDLGLPKENCEQLSKTFKQYKDKLQQIYENQIIRIGKAVEFHYGISKFIVSESVAPQKQIEGNRFIDTNITFQHDNGKQECCQFVIFPEQLEDMLNSFKKAQKLMMQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LY37</accession>
    <name>A0A8S1LY37_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCAAT-binding factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0480251</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LY37"/>
    <dbReference type="OMA" id="ISYGSEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027193">
        <property type="entry name" value="Noc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455:SF0">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="2">
        <location>
            <begin position="219"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8072139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="375" mass="44116" checksum="C0F30B3ADBCEBB0C" modified="2022-10-12" version="1">MVILKLASEIIEDGNLNKLNSLLSMPLTQELVQTLRRIFLYLQDKKLFVISYGSEWNQWNIKKNNNNRQNESNQIDNFLNVKWSVFLRLLFQHSFYNDLLLSIEQTINCPPYVRLVETSVEKGFEIPIHSNYFEVLQYLTFDFEKFIILLKRFPEIEQKDKAQFTIFVQKQLSKITNNQERLSFSKLINTHLVDKVDNPLILADFLQHSIDNGSLELKINSLQSLLILITRFNYNLENFYERLIQIIDTEGAFECSSKSKLFKLIDVATKSSKVPLQTLARIIQSILRAFLKESCSLQLAATQLIFNIFKKHVTLKQFLQQGDTQLVELASYQNHWHPEIKRIISYLEDDLSIFDYFNLNDVIELQDQQLLEAQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZ14</accession>
    <name>A0A8S1LZ14_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0460156</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZ14"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="123"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8070823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="488" mass="57120" checksum="EC9D8A30F105A4AF" modified="2022-10-12" version="1">MRQKSQTVHEGGLKKLQTVDYDKCIYNCLLIKQKFIGRGPRYIYLFKNQICIGKSPFVQIPERQFQINQDMRIQWSYKKNQLKSVIFQVGGEQFEYFGTNEQLRELKQKCALYVFQVKIQDEYQAESILGKGSYATVLELTNLYTQKQYAAKCIDQQRINEKKNGYKQLLQEIETMRILSEIKHQNILQLHELYIGNQNYYLVMELAKGGSLLSLMKKRQTLFSRSDIKIIMKQLLEGLTFIHSHNIMHRDLKPENILFMNKDLESLVIADFGLAQSVDSHPYTYPKCGTPGFVAPEILEQDSDYAKYTVLCDIFSAGVILYVLLIGEPLFEKKDRKEQLEFNRKCDINLNKFSNDQLNDIEKDLLIKMLSKNPEFRWSANSLLKHKFFISDDQIESEIDFYKLNNMAILKKSMMPTFLKNPFMQNVKNQNTIRDKSISLINRQRNCTLVMQSNLRFDKSSQEQLNTSQINGIGSFGLFKDEINQINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZ79</accession>
    <name>A0A8S1LZ79_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZ79"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="241" mass="27925" checksum="9D8B7EB7911920A2" modified="2022-10-12" version="1">MGNCQACMNDPQNLSEYGAHQNAKQTSFYSIVRRSQPFSKASLAEIVNTSQHQLRTSQKPSIHIQKKASLSMVGNGVDIQKQKTNSEQLISVHCPIAKLAQPWGRPASELQSLIGSKVKMVLSKLKEYQYIIELKLNSIFFYKIYYTKFSIVFQLQNIEIATIKNNQLILLNILIKQGLCYSFLFNKPQCYFYQSSKPFLHLFIYYCSSDLTTQKKRNNENYIYMFYEMRAIHAPSPKFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M377</accession>
    <name>A0A8S1M377_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0520088</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M377"/>
    <dbReference type="OMA" id="PSIHCYS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="343"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8074119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="469" mass="54448" checksum="6B7C75C3A06178DD" modified="2022-10-12" version="1">MGVCQSHSDGKKSNKQQQLQIVSEQTLQEKVKNKDNVIPRHKTMPSIHCYSKEKLVSCKSMIVSKIKKRVYGEALAKFYSVLNSQFGYKNNDNMGLVQHNVTGVVRVARNILKPNEQDQDFDHLITNLQDQSYNSLHFANIIELYQDSKSYYAVQEYCSGGLLIDKCGLIKEEEAIYIISQILDILEEIHKQDNYHGSLSISSFALYDQTNNHYVKLIDVIPIFQAKEKSKISEMQKDDRQAVGLILFQLITNKPITSQTIEMIIKRPKDLPTKTEWYQLVVLLLESRSSIIYEQVLQNKQYREMVQKQEARYTETIINKSVKKTSYIQQKVLIIMNSIFFKERQQQLEKIFKKNDGNRNGTLSKEELYTALGTEEIIDDLFENIDCDGNGEIDLDEFILHSCDRKSLITDFNLEATFKELSRKGIIVASNFSRLQNCDEEKIENDMVEFCNSKRMSLEQFKKLMMDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M6X2</accession>
    <name>A0A8S1M6X2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0540246</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M6X2"/>
    <dbReference type="OMA" id="VRAMYNM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005052">
        <property type="term" value="F:peroxisome matrix targeting signal-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002661">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024111">
        <property type="entry name" value="PEX5/PEX5L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130:SF0">
        <property type="entry name" value="GH08708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR PEX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="352"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="464"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="498"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="532"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="217"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8075519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="639" mass="75289" checksum="FE8F65CCEDE77AEF" modified="2022-10-12" version="1">MAFRNIYTGGACTADGTQQMSQNPFKQFMDRMLFGQTQQQQIQQQINVQQDPMVQQKLQERQRLFESMNQQWDVMAKQYDQANIEQRQMMEKMFVEEQRKYEQAMIQQQMMMDFQWQQAEIQYQADQMAQEYLFQEAYENAEDVQAQSEKSQKFADDLVQALEKDPDPRFQQSKFLRFMKDIQNGNLKLVGNELLTQKGDKVEQSALDRIQTLEEGWDQATKNVEQKQSDLTEVEEKFSKMWDEKLEKYENELQNEDQFQKKLEDTYQQILKEMDTGTDFDKMFSDAWQAASDLQEFELYKDSNDTYRFQENNKYLNMDFPLQAALNLVHQGQNTEAILALEAHIQKNPADSNAWRILGRLHQENDQDQRAVAAFLNALKQNPNELDTLSALGISCTNILDEVKAMSFLKQWLIRNPNYKVAVDDSIVPDNTNIYDYTLDQIKCMNARMIQVFEAAHQQGPNDVELLNGLAVLYFIERNYQKSVEIFKKALQIEPKNYQIWNKLGATLAHLGEADQAMFCYHRALDLRPNYVRVWVNLAFAYSYKGEYLDAARLYLSALMINPQAKHIWGYLQTAFLQLQRPDLISKIQHYDPMLFKDEFNVTNPNDLPEPEILYREANNLYIFKMPEEEWLQTSQKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8H8</accession>
    <name>A0A8S1M8H8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0470229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8H8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="119" mass="14243" checksum="A9ECAF70CA645967" modified="2022-10-12" version="1">MKRRINTEVGDFIRKDLKNDFNSSYYNFTQSKRYLRLKSDASESTVQLNRSNTINPFIFQQELISIDQTKLNNMTKQSIKGQTQQMKNLQQQDLNLQKKSYYLNKNLDRFEHKIVVNKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M949</accession>
    <name>A0A8S1M949_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0570228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8077128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M949"/>
    <dbReference type="OMA" id="APIPQYV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="262" mass="30157" checksum="EC63903E3150FB59" modified="2022-10-12" version="1">MSNHPSQRPPQPVQNAPQVPVAYSPPRTYAPPMQFASPSYYPIQQSVVAAPVQYVPQPVAVQPIVQPMAVQPVAVQPAQQVIKGESRIEYIPYEKSVIEYEEVRQRIQVPREKYVTEYQAVEYQTEYVPQVFYDKVTEYVPVDRYQDRVEYYPVERQVVHQQQVVAQPVVQPVVQSVVQQVPQYVAPIPQYVAPVQQPYVAQSYVQPSFVPSRVAPVYNHAPYQGRPVSQPRRFSPQSKPVQIQKQQQPQEKKKTFLENIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9B5</accession>
    <name>A0A8S1M9B5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0520254</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9B5"/>
    <dbReference type="OMA" id="NHNDCFS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000123">
        <property type="entry name" value="Potassium channel KAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="443"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="545"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="746"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="808"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="885"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1037"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="981"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="808"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="825"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="851"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="909"/>
            <end position="926"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8074393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1057" mass="124204" checksum="8DE2A02DE4B7FD55" modified="2022-10-12" version="1">MDSLQIQKQLMVEQLNKLEYMDKILPLHKDEVQAEKKLQVQINSLNFKRIIEGQENNKLMAEIEESKLDVQKNRQKAFQKYFTSFMTEKQQQDVNILEFNQSDFEVFQQLKERQRQLAQEDLTIARQKEKSNQIFEQNLMKKWDRVRKKLRMIGRMKRLYDEVKLYGTSCRVETSIKRHEVIQELEQMLNKQNQQISKPMPQKHKHLFLPDSKFKKYWQYVLMILLIYTAFITPIRLAFIEEFNIIWFSLELIVDILFFLDILITFNTAYFNEEGVLIIDRQQIANQYLKTWLIFDLMAIFPTDQIFENEAQKLSQYFKLTRLPRMYKLISISRMWSTVQNSQNHNDCFSAIQDLINLNSTTVRVLKFFGTVLVCVHIMGCLWYLVAKMNNFGPNTWVYELDLLSKSEYELYLTSIYWAVATICTVGFGDIHAFNDTERIVCIFWMFFGVGFYSFTVGSLSTLMGTLDTRESHLQSKITFMDEFCEETKLSLQMKHKIRKVLEYNSMINIFSSAEVDEFLSEIPTNLKYQIAQAMYAGLKNRVSFFKNKDSVFISTLIPKLQPLKINAGEFVYNKGEYPNQVYFIVNGRVNMVIGVYSITFKTYVTGSYFGEIEIFDNSARFHSARAELECELLAIEQDVFKKILQAFPEYFEEIKAISLEKLKREKDSIEKLQDLTGLSQTSEFFNKKRTQSIYKSIKQRESVHFIEYSEESEQDMKSMRSSKFGFFKKKTNHLIPEETLTTRLHTQEDTSQKNLVVSDSSQKKNEEPEKLITLSIPDQLKRDSEHKIKLYGEVPIAISQFNEDLDQTQQSKTNQRIQSEEPNRSTQTKQVRYISQNLRCSPKRKEQRKPSIQISSTNEIPSSDTPLISSVRSNFIKPSIKIDEVSHESEGSNKNSPNMSMHPHIAFSNAQSPQNSPRRNSTQSKFPKPSPFQKQQSIFKNVSNQYIEASKLKNLPRLGGFRRTYRKEREIEVLEETIENEDYTQLQQKLVELLEKIKKQKEQQKVKKQQIKFNIINPLPKNIKFEQKDSISISDLPNVEKQQLSQQDSNSSGILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9B6</accession>
    <name>A0A8S1M9B6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0570051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9B6"/>
    <dbReference type="OMA" id="PIQMIPN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="394"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="222"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="409"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="432"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8076777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="457" mass="53219" checksum="B752E11AEB5D8BA5" modified="2022-10-12" version="1">MDTRQSEHSRGKSQTQIKLRNPFELLIKIIRLPNVCNKCGHITRMKEISDILEQFDTMTSQQIDTINEKLIQKSNQILAARQQLVKDTKNELQQSLQQLIQVRQSMGSSFNFEDKEKCLERVCEDIFCNVQEQIEIQNSLSELNSIIQEQYQKFHQFPNEITEMNKLEYHDNLEIRERLDGALRINQSHKDILKEILIKIGEENKSYTFDIQLQFRQQQLEMLNLLQCNQEQIKEIERLEDELNDKKLELQKYAILFSQFENPFKSNKISSEESSASNFQSIQKEIIQQPPSSIQQSRNVFQDIKNIQSKRFSQPSINENNNMCTFQSEQFYSIDQSHGNLNQESSQKSSQNNSKQIENSVVINQRYGNSISNKFNNSKATSLISQINSQQNNIPIQMIPNSTKKIRNSVDTNPMMSQESFDNQNDNDENSPKITPTSKTLLSKVPPLPIGRQLMLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MD73</accession>
    <name>A0A8S1MD73_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0600053</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MD73"/>
    <dbReference type="OMA" id="NANIWDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="428"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="718"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="817"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="864"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="722"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="817"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="884"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="905"/>
            <end position="917"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8078450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="977" mass="114841" checksum="C33CB7235BE9846B" modified="2022-10-12" version="1">MSQRLLRKSRTNLGDQSQSQSHSHTSPVMNDDGLRNFRIEFSDLSRKRLSDSGEMKDPTIYSKKSRLSQFSNINHRQRDQSDHNISVHQYTATTKQYYQLKWKELSGLPKQTDLQQILRTFLTKLKRNANIWDQINNKYCYLKESYLQDEVRLQSQYISYMRQSFHYIRLLSLLTVPIQAGSLFIDQWIQGLIILFLVIEFIDQLLKIKLVKLNVVCNFIYIASTLLLNFDLNQEGKIVLMILSLTGVKYQFQWSKSTIAIILLIYINYFANFWLLINDNELTYLDSIIWTINNCLPFNEVDTQIISYRNKIISVVFQILSFYFQILFISILVKFSFSTNQQELFIDFLKVNKVDQNLIDEAQHILNSNIHSDELQTVNNLMQQLPINIQKPLFFLIKNKKLQEIEFFTQYFSKQSIDEITFESVLKFCKPNEIIVEKGQHDEYIYVVLQGEIGIYDNNIYLQSLPKNQAFGIENLIMNQGHNLTMRSKGNSILIQINHSKFLQTIKKINNDLEIFHSIKNEVLYMNRIELLLQQCYVCGSYQHLSSLCNRVHINLNRNLVISRHLYSKPQKRQKYDRKWDQRVNSLYNFKLVRSCGRRLKRNYGINFVDPEAYERGEQEYEEEDDEEVYQGHLSYKDLTNPSRKSVEVVSGQTLRDIIYHDHIDNEEQLQYYQQQQEINRHQAIKQTLQTAGFQNEDLYFGDAINIPLQHKQFTQNSNTFKSSSGQTYQSQKSQQVKQQQQNQQQQQQAQQQVPYQSKTYVQSPLGTNQFLIANNRNAQPKLTFSYDPFYHEQNNKQTLPQPQPEYQAMIRPQFRSSTNYIPSGPTQLPVVSEERDSQLNIPQMNVPYASAISISSVGISSIDDIQRRDGQNNTYTGPRRMTQKQTNTITSTISPDIQKKKSISGSNSRSYSALSSRQNQHYSDLDNVLFRYDVKDNFDIDQIGIYQQFKPQNNYDCVVNQIKIQSKKLIKQKKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MK09</accession>
    <name>A0A8S1MK09_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0640065</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MK09"/>
    <dbReference type="OMA" id="PRLEIKA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="494"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="435"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="494"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8080638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="537" mass="62606" checksum="25B70F4DA21E4750" modified="2022-10-12" version="1">MGITCSCQTKIDDDYILDDAPLNEANQQQHTDLLQNADKIEGKEQEQQKEQQTIDIDPIQLKSYEIEDNCPQGLQPLSFQAQQEFIDQYPAIVKKQLQKLPRLEIKATLTPNYKRIPLIDTPYQLLNGDVYVGQWAEGKPFGLGKIYYLDHQVYEGQVVDGVPDGEGRKIFKDGSFYTGQFKMGEINGKGKFTRQDGFKYEGDFLCGRPDGNGIETWPDGTNYQGQYKLGKKNGKGTFTWSNRKNKKTGKLETYTGFFKNDVFHGQGRYEWLDDRIYDGEWVEGRMEGKGEFIWPDKRKYTGHYLKDIKSGYGELEWPDGKKLSGQWRMGKLTGIVTLTIKGKIKEGQTEPEPDKIFLSEWEDGKRIKWLDNQKPSGIHSNISQLRNFDEHQQNTSQIIKSQIRQDDIQSNNKDQLLNNSEVQQQNTVFQQNGQIKQIQQQQEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQIIENQNNLSIKQNQQDNISSNIEDNQFQQSNDNKQQNEKQNVLIKNFDQQQQEVQKEKTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLV7</accession>
    <name>A0A8S1MLV7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0590019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLV7"/>
    <dbReference type="OMA" id="GCGLESY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13515" checksum="F849D7F336313988" modified="2022-10-12" version="1">MGSSSGIGLQPTTNYIGLNLDSLQNLIWQVSKLIYQIFIYWLIQIVKLWVILKLQILFAELIVFVQTHNFLNNENIYKELYEKKNNITKVITRPFGQVKFQVQTNIIGCGLESYSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWN7</accession>
    <name>A0A8S1MWN7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0720191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWN7"/>
    <dbReference type="OMA" id="FEVIGKC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="81"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="418" mass="49159" checksum="D89F010F81CBBD0B" modified="2022-10-12" version="1">MQKQTQLTKFFSKKAKEQQESPIRTKLNDFVRFKVYIWQMLFCTPPTQEIQGKTENLNAKKKCSNNLDNWIRKSSLQQKKWPFLPKNLMYQIYKFLQLRECATCSQVCKFWMICYNDIYFSYSFFREFETSRFNEKELATVLKRSSKQLKHIKKVRDIIKGQKISSFLEKTELRTMLSDNSQQQGSLLELFKLEPKKQYTSVFLTDKDLNSICKESYKSLQYIQLISCQFMTGKGFEVIGKCSQLSLINIQSNCNLKDENIKIILEQVQELKTLMLLKCDGLTNQVFEIIGNHSKKLERLDIGCNPQLDLIKCSPLKRIESLQSFSIKENIVTNEALEIITTNLYKLHSLNLEGCLEINGYSLLQIQKLNLQRINLKKIQFKEEEIYNFILNTKSIKLLLVNNNLLSTRVEKIILSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZ76</accession>
    <name>A0A8S1MZ76_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0680134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZ76"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="71"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="292" mass="34630" checksum="F9317FFFE4BADB9B" modified="2022-10-12" version="1">MNPETDQLNYDQETPQIPYNYDVDGRFFLPISNQIYQKCYSQYTYHPPTFLLMPQQQIYQPCPQVKKQKKKKVNNKEVELLRARIMELEMRQPEVKIVKEVVTDTEQITKLEKELRMVKMQLEHEHDNNAQKDRIISELRAQIQKNDLSRSSSLVSLQTTLVERERQITKQDGIISQLQSEINLLRGELDDAHHHIDELQTTHEEVTVEKMTYLSKEAETWKQKFIILNREYHETQEKLMLTEAELESIKKGEVKEVKQIVVERKDNIGRTVDYKQSEQVQVRKSGFLSTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5D0</accession>
    <name>A0A8S1N5D0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0730068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8084946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="EHRIISH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF112">
        <property type="entry name" value="BEACH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="2">
        <location>
            <begin position="1934"/>
            <end position="2046"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="1">
        <location>
            <begin position="2050"/>
            <end position="2341"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8084946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2692" mass="320127" checksum="756BE5B535441E18" modified="2022-10-12" version="1">MLQFVPQYYVEILKNNLQSNQPKIYEAIEQQQLDYALKLLNQQQSPSIWISSIYLILYKQDLEQNSLNQFVKIIEDSNISEMPEHELLAFTQICQQIMNIDLIKLLYKYNLNGWIQLSPKIHLYLRKSIHPKVPYIFINYFINQNIELLEQHEKSYEEDFIWTLHDFRSFYYMMPTDINHPYYQLLKISIYVRMLDLENVPCPISPETIPIQYQSKLENILKQIIHTPHKECLDLYIFCLIDLGLFKMVIQHLQPQLTFEQINQIRTSFIKHANQSNYIVDVDIYHYLNELQNFDQEFKQLIYFNNPAIYEKMDNFNENWILNGLIHNFNYIQSQQRIIKQLAIDNTQQSLKTLWILHLDGANSDLNIDHQKHFKIKNFKFLNIFHLIKFKNDSDFQHLIEALKVIAICIWTDREVKQEDFENKLFLVSVRKCINIDNCSQIFQLLFGISSGIFENQYEIHYIKIQIPYFLELIIILLFEQNIDKNQKEFYLNQFIILTQNLENVLIILKSQIIERLIVYLRIENQYQDKIIQILINCIKVRFQLQYLQSIALFMSPYQSLIMIPYFDDQQKYEQYKKISKMLGIPKQSIESGSLQQQQYIYDSISNNDLFIKNSIQLLYCLKEIVSIQNDQYYYFTGNESGIVVKGKILLETQNNSFSIILQFKFQIGSATLLNWGNQDINFSISIEDSQFLIIKFKYQQITKVAKYILKYLDNFIVINFQTVKQIFVNINGIDVLATQSDQFDNFILPVLNFICVGAKFSDQEQFQNSFRGEMRMLYIIDQNINAEDIYILEKKSQNNSRLFQQLLKMQLQGRILSYLSTQSQKQDLHYDGNLHQSNQIILENCQYLMIRGKRQKQNTISKFMDKFFQQRKSSTSLEVTSLIPKNVNQSSKNVFFLENTTLLDVMKSYGNLDILFFPLHIINDQQFVIAVLQLLNTVIQKFGDDISIQNYLRSDSEFKGIKVLGYLLTVFMKNQGCNKQLLQNILNFYHSLLLCQIIQALRNDCMSIYHEFEYWQYCQYNELHFLYDYLFEQLQNNEELYSYYKDKINCLYYILQVQLIKYEPQKGEYQIFQQIRKILIKCILFLMYNTFFQIQYNNDEVVPVQMIFRDKKNEKYSTEKLFVFKNEIILLLNYISLKSVSDLAVILCFMLRKPQNSIIQELLSEIKCGNSSAQKESILETMQTQFIQKSESEDTLLVIIFKIIYGVIIEMIDQKCLDDQFLAPLIEILVKVQIQQINYEHKLLELQQPKNVQQLQKTKECTLIKSLFNKIKYILYEYSNDLICSQNTYQVLINLSQLVLKQELNLESDVLDLFLQYFRKFNLQTKELIIDFLLQTSTYTVFMTKLCDYKDLTYFINELITIETGQTLINYIVLHHIDNIAFSARKIVRLFQKVEQNNNQFMKVLQKILILQNNNSNQKQNQKSIQAMIDLFMLLPTSLILNKNIVLNDPELFIKVFDQYLIYLEKQDILYSYYMDTEFVGFYEYDPIPFYIKLQIDVQRKYLYANGGIMGTVLFILFYSLELLMSKKEAKSLLIIWKKLITQKQKSPNRPIQQQSLIMDEGIPNLQTKIQKIKIKQQKQSLLQKLKQNILGDYQQTQYDKYFQDIHLIHLLKFQLLSHQYSHSEQLFEIFSELQFKPDNFINFQCLWNIVEQKRILGNMDIQFQIDQSNLKQITPDINIEAINNKGYFIINQFNQQNQEISQAIKENGKMSNDARKFIEMIQKANTTLKYLELFKDSDLIKRALTYMKLYAYRQLQFCQEISILLNLHSIQIQNLDQINNQIVQINQQSQQNNIEFSKLSYNLFNQYRQDSKLTILKFENEQQIQQLIIQFKVKLHFKQNTQKRGLWYFMNYGDAHLYQNLNQVNYDTFLELLGKERYSINSYEDSLRRRMFLKVFAKQKKSYEHIQLINNIQVVFNDIFNIQQQIQQNNNKYVVMENQQFLAELITQRGFYRGKIRITNDYLMFENFGLDLQELEVTYKFQKDSLLKEEKQKLIPLSQIKEVFTRMYLAQSTAIELFTSNNKTYFFNLLNNRSSVLKILSQSVNVIINPCEQFKKSGIMEKWRKGEITNFQYLIEINKYSGRTYNDLNQYPIFPWVIANYQEFDIQNKEHFRRLDQPIGALNQKRLENFLERFKEADPEDMNMYFIYGTHYSHSAIVMSFLMRMEPFASLHQELQSGKFDKADRLFHSLEQQWYSVINSSSDVKELIPEFYYFGEFLKNKNKFDLGQLQSGTTVGDVTLPIYINTKSPEELIFLNRQALESEYVSSNLNNWIDLIFGYKSGLNGQKYNNLFHRLTYSSYVQQLLDKIDDDIKKEQYITQVNYYGQIPQQLFKKEHPQKSQSKISDQSPIIQFKYITQIINKQSNIIDQFFCNEKYFLILTNEQEIFVYNVEHRIISHKIPRDFSNQETKQIQFNSNNVFLLFDDSLVVAGYVDNSVKVYSLKDLKQTCQVSFHTKVVTCLGQSTNYLLCGSMDTRVSVWEWTLQHQPEIILYGHQNEVSIIQVDSILEIILSYDVKGDILLHTMKGVFLKLIETSLQDCSSIRLHPSGFILLSFLNQISIYTLQGELYIQRELLDHIINVNVINEYTPEILITTLDGNIFITSIVQLKLSDDFTRYCLKQYKLDEMEIKLETNQIKKVKEISGKSADFLNAYIAYSQYYQLQAYRILIIGYQNGQVISVYEQPKQTSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N851</accession>
    <name>A0A8S1N851_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0780155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N851"/>
    <dbReference type="OMA" id="KVFRAFP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="48"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="168" mass="20090" checksum="D11EC08BFA3B486C" modified="2022-10-12" version="1">MDISTKASLTIFKKKRTNIQTIESIEPTSKRQISPQHNQRFFQSNFSSKAMITNINKEKQRIKQLLEENVKLLEQCSEKDLQIGRLQAKIQKNVDLRTQRVQTLNNLQKIEKKNYFFDNQNQQNISDLQQNEDKQLFTFYSPKQKQNSQEFKLPKVFRAFPQQNKYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9K4</accession>
    <name>A0A8S1N9K4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arylamine N-acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0830209</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9K4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF0">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="32350" checksum="91974F57E54B7BFA" modified="2022-10-12" version="1">MKILAHIQKVLQLPNHIKLSFSVETLYLITNKQIIYNYYQNAELHLKEFKPISLEFFDILQRLCVQRRGGLCYEQELLLYYVLKHIGFKVELIRCQVREIGSLYNPDLQSTHAFLYVQIRDETYLLDPGFGTRSYRFLIKVNFQNLSQTYQLFDQEHYRIQQNEAHYQFQHYIDDNWQPIMILRRHLNFVLKQFLGIRDNRFVICRNTEYGKIQYLWFRLEKEFTAFKKVLKFNECSNIVFKSYNELKEDVKNEVGLELPDYELLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBD7</accession>
    <name>A0A8S1NBD7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0850207</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBD7"/>
    <dbReference type="OMA" id="CWIRIRE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05462">
        <property type="entry name" value="Dicty_CAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8090317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="29598" checksum="C94CFFB766CDC94A" modified="2022-10-12" version="1">MPNLIVFDQCILIIINNVQSQNIQRWTAIIAFILYQSCIKNKNEQSFNICGYLFIGFCIPFSISIYPFFTHDYGFAGISCWIKEDIKHPVRSTIVRYINFYIPLILIILYNSSVYIFIICKLYGISQQQTKFIYKMISYPLILIICWIFTAFNKTYEEIQDQQQIWLLYLSKALSGLMGFFNYLGYGFTPQVRDQCARYCCKRNSKSVSVELELKNQQSLDTSQNTEEIKSRNSVNSQRDSVQGELSFIAELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBH0</accession>
    <name>A0A8S1NBH0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0850026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8089958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBH0"/>
    <dbReference type="OMA" id="KCDSHTS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="308" mass="36990" checksum="C967DB0781E32408" modified="2022-10-12" version="1">MLPLLYAPQYMNYPQLNLQQFYMLPTTTYALQTPKKETCDQACQFPEPCLFESQLPSNTVKFEEAAEKELTILLKYLSKHISLLKDSVFDEFVLENLKTLSSLQKDLPNMIKKRYILVNKTKEEMTKFIIRRCFLFIKTQIDYEEKEGVSAEERDRIFYHSFFSDDQEFMKSLQNESIDDMIPFRKDSKVKTMNDNYLKRLFESERFSKYYALFLNQFKEICQNENEEKIENMTKQLIKIIMTRDYGKIKTYRRFPWKDHELIKCEGRAKELYTKYSNSHIKKTKNKYSSKCDSHTSDILEKYSESQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDN0</accession>
    <name>A0A8S1NDN0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0810039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDN0"/>
    <dbReference type="OMA" id="MGRCTSR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10229">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187">
        <property type="entry name" value="ALPHA KINASE/ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187:SF5">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="582" mass="65997" checksum="8F928174674F5000" modified="2022-10-12" version="1">MALLKDDTAFTHVIAIDFGTGASGFGLAPKVLDKEGKPRIEVFNPCDDSDDQKTPTAILFDNNGQFIEFGSQALQKYASILDDGDTAYLFQNYKMHLYHMHNHAKSLDNRELPLMLLIKETLKYISNKAIQKLKEQVGKVISTKIRWVLTVPALWSEEHKQFMRKAAVEAGIVENLNSPNLLLCLEPEGASIQCREDAEQTLKEQMAKSSVVMVLDCGGGTVDITVHKLLCEPNEKFLCNELIPSSGGCEWGSKYVDLYFEEFLKEFLGDKLFRCYLQNAIARLDILRDFEILKRKFKGNKDERCMIKFSYLGEELNTAKLTQLVKEHNSKHAAEYQVKLKGLSNVEIPSALMASFFQTLFENIKNKVAQLLQQVEQKKEKVNFIFMVGGFSESPFLKSEIIKRFENNAIQILVPRRPQVSVIRGACLFGLSPRSITSRIAKKTYGINTLTTFDAERHPIKKKVIIEGEEFSEDVFDAFVRKGDAVGCDEVHTKIYCPVRSRQTIMRIIFYVTEKREVEFVDEQGVQQLGELCIDIGKPLQSVEDKTVKVTLLFGNTCIYATATNKDGTEIKNCEFKFECGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEU1</accession>
    <name>A0A8S1NEU1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0840116</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEU1"/>
    <dbReference type="OMA" id="WTCISIL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01610">
        <property type="entry name" value="PAP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF28">
        <property type="entry name" value="DIHYDROSPHINGOSINE 1-PHOSPHATE PHOSPHATASE LCB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="73"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8089789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="413" mass="47720" checksum="0B33B99098B93A8C" modified="2022-10-12" version="1">MKLFFIATIALLSYIAIDLVFNQNMWDANTTLTLYLQQNQFDGEKEMFLVFSYSLYVLPSIAGIAFLILDNKLGALLYGGMILFSVASNSLLKNIYHQARPFFIVEEIEPYECNKEFGKPSGHAMTSQAMCFLLPSILFPAIWKDQSNCKYPLYLRLLVIFVITFWTFMTGFSRVFMGVHSFGQIILGWVYQGYFSIIYMQYLHDKIVNYLKECLQVGQQGVSIKIISVIGFIVFIWTCISILFLELNRNIFISTSEATLWMEAVYEKCDNQTTLYTIDSPSVLQNICFSMCLYIWFMLSFVFGIKLSKGIYLENEFKQHYKQLSYWKKTQRILVLIILISLLIPFYFIKFDAVYAQAFGQVVPVSILGGLFITVVYQKLLNYLKISIPGDFLQIITNQSSDGIIIERQVKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGJ2</accession>
    <name>A0A8S1NGJ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0860008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGJ2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="18624" checksum="5D3A39FA4C14749D" modified="2022-10-12" version="1">MLQVVIDEQLNQQFNQKETRWSNCSPLYCICLKSNINQQLYQHRRYYHEKVDLELIQNNTNNIVAYQRELKQQIFNIFMRRNQNNLNYQAVIRITIKISNQSQKLGYSQIFDPNFHIKRFDWNYLNMIVKISYYLFFLSKITIIMSIKNHFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NIX3</accession>
    <name>A0A8S1NIX3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0850088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KREDHYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13595">
        <property type="entry name" value="ARL6IP4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13595:SF4">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_77, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="791"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1184"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1232"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1529"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="819"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="791"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1529"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1565"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1588"/>
            <end position="1621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8090081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1628" mass="192437" checksum="BDECDC839E431A36" modified="2022-10-12" version="1">MKVSLDHDVYKWLLSLTIVKPQQVKNTGKVELDENQSKLFVNGIKFGETLNKMLEARSIHVPNLSEQMKNQITPGVKLFNWNILQDAFQKINLPLDNDIKNLIVNGDTEMMNELLKDMMELDNQINRKQQTRGSVRSSDDSSFETSKKPPSRPASKLPQTKYDIQGNNNELILDELDTKKPLNESKSLLEFFITGLSKHLTLKPQQAASLMSNGNKYLAHLLVKGVKGEFETLINWLSDIYANISRVFNFLEQQDSNVHYFFATIKPGLLSKEQEITLWTLRIMGRTYFELSEMEMHQLGYEWFCKENGGLAATFLCMQRHPNLLSQICELYSQVARFNIVDFFTVQFKKQLSDKCIDMLNSLIEFLHQQKILKEELIQQGIIQYWAQAALKMSDSDQLPIKMQGLKYLTLNWLLYPIQFEEDQNYPNQFLFLMKKGTRDSSNNLKYFSLNMLFRLLDKLATDRNPYAAVLYKKLTFTLIENYNELDIREFMLNNFMYLIQKYSSIPIDILLEPLIKQVMLNDALVNLTDMMLFKFISKHPKMKIRLAILLLDLLAKIYLNSVTMSHLSLNPIQVLLARFAESPLLREYAFKLCKIGLALHYGSLKSKRQQVKTNDLSVLTALQQQNNDHEILEAQKRAQIIELIKFIINMQQDELNEQIKPLLCHFYIELQNISIKDKGVITLLNMFGNPEKIVQVIKQQWDAEKQEQEEILKQSQLIIDNEFVQNQQYLHSAEQTPQTVKSEKIKQNDVDPFNDLRQKELEDEKRFLAQNGVGFKKRVVELERGKQPIAPTPIIQQQQQQQQQVKKKPQQVDPKVLENIANIKLKKEEELLKKQQEEEEKRKRIETLQKKAQQEIQKRTNGKIIEKIDEKKVELIDLDDHETHDYESMSIAIKKNHKAMKYLFSKYSNTTNQASKKQYFGELQEQYEIIQLSDIMRMLKDYQVNMSKEEVQAYVKAINQKYSENKSDQFSLDQQNFERFLAQLSMKIEGYTFQKRPAGQCLEQFMQSFGSIAKSKNENHLIFLDPDNINIDPDRDLIRQLNFQLQTQDIPVPNKFKKVTEKQLNQRFEYRQSAILGIENAWLDCHEIVNQLISETFKTNVMEPISDVKIIWKVKPNPQQQQEQVVQVKQRDNSQQKKVINRAIEDKLLKQQEEEQKKMEAEEKRKKHQEKLKQEYLRKKKEKEEQEQLKKQEERQEMIEKAEREKKQQELLKKEAEEKKLKLREYKEKMAEENKIKEAQNIELQKQKQEQERKQREEFQKKQKEDVSKLLEQKQKEFKDKEQQEKQKEQKAKEEIKEWKNNVEIMLQQEQEVRQREKQMHQKIQQLQQDQDIQSVYKKYDKQLQAIYHAYQEYTDWKIENAGVEKELLQFKGFTNFASQFSIYPAIITPEDAQLIFRSITRERERQQQQLNEKNGFNAAPPKGMNYHEFQQALLRIAIKGQKYFDLLNEKYENNMKTIDMLALKKDQVISEEGPLEKREDHYQQVDATSSKTMQGLMYFLDMPEEKTEMNSKLRALKLEFQKVAAQRDKKQMVQQKLTEEVKQPTKRKQSIGQKEGKDKSQEKQQPQRNKSQNIRIDSAQTKMKSSQKDQIKAVDENELNKQSPKKIETTGPDGKKESWKVNQQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJP2</accession>
    <name>A0A8S1NJP2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0830070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJP2"/>
    <dbReference type="OMA" id="HKIKKDF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="676" mass="78337" checksum="866B21B67A9AB22C" modified="2022-10-12" version="1">MNPPPGLCDDDDNQIGPPPGIDIPQKKKFHVNKKQSQQFQQTSLESLNLELKVTVQDQQFDQIPISDDYLEPSMNNLIAFNGYLCDTSGADSLAIWDKEMKLKKEYKGLIENMPYQMVAVHDKIYLIFGDILMMVEPETNKKTILIEQALTAFCVSDHPNPRIIGVQDGELMLWRTSNFGKPNQMEPKYNNFEPKTKKMIELQKVLSEKFDDCDDLLMLQNGLVFGKQKKVGEVFIIDFVNDQLEELQTTYGCAVTCWTPISYDSVAFASNGQIVITKFQSNKQAVVQIILNSSKDDIHHGLCYTNNQLFSISKNAVVTSWNIVLGTKTKTFEVKFCPGVYSLITMNQEIIIAEHKVKKDFILLNTNLEKQFFYKARVKQVLHCCSSPNQQFLAVVQKCGLLYDEEVIFHIEIEIYPFDNKLEFQRRKEIDNLEIKTVMFLTDSTIQCILKISKSVYPLLVKWNFITNEYSESEMLVEKYKSCQLYQNRSVFSYDFHNPPSQQFNLFAPKRVDDQNQSTGISVYDENIKRLLKVQFQQQFNADVYGMPTPDLLYVLNNKNQIEVWDVVTFQQVQKADCRPSIIKQIRFSQQYLFLNAQSEWIIRDLKLAQICKTKGDIINKCLFLDKKCVINQKQNLYEWDLVNNQCKQIPLFKVYDFCIIQDKILAIGESTIKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPP9</accession>
    <name>A0A8S1NPP9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0820001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPP9"/>
    <dbReference type="OMA" id="IMISCEG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="243"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="288"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="440" mass="52280" checksum="2CB1BA89CDFC9F80" modified="2022-10-12" version="1">MENLASKSKLMNFKKILEHIEETQKQKKESIENMLIINIKQLEELQRAFHQLKSNIVQQLDYLIGNTNEWIKQIQIWGQLNVTYSFFDELEKLINQTKLDQTNQKSIVDQINQINQSWNQKIFKKLNLFKSFQETQKCEELLISLQNINFINEKEILIKENQENDNKKQKEVIQGYIQQNQQKQQLIINKQVELKLIDDSIQQSGYCRTIIFDNTGSIMISCEGKEINIWNFELGRLTLINTYSQHIKAVTCLVYSKLRNNFISGSYDKTIISWQQINQKDWKCSQPFQQHNDYVNCLILNKQEDQLISGGSDKSIKVWNVDFIKNELNFQYSLDHHRNIVFSFCFNSSETVLVSCGYSEFIIWEKGVLKNKVFSIFQMNLINFLISLCIDKMFQIMDINYIQLMINNFFGQLQIRMLMIFWCLKQRIESFNKILIKLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPQ5</accession>
    <name>A0A8S1NPQ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0950061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPQ5"/>
    <dbReference type="OMA" id="WDGMEEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035909266" evidence="2">
        <location>
            <begin position="16"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="267"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="423"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8094222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="497" mass="59486" checksum="D7436B9CD0322CD7" modified="2022-10-12" version="1">MKYFILTLFVLNAYSFSLNDDWDGMEEAIKVQQDAILVQRIIKQDLVDQLDVYSDTPITQQIENQVQQLMNPSVDTDKQETEDEQTEVHSLEYWLERLGDEYSDETILLQQSQQITTDVDNIKIEDINLISESSVIINNIDLEYQDQNMNENIQMEQQLLNSAIQLQESLNQEDQQQQQNESYQNTLEVQINTINDESISKFIPNQDNNRMVKFLNNIQEDTQNYMQQQQQIKEIYNFQDYSFNKQIGEQRSIYNDKEDSTLQVVERDQHKEINKNLQQQYQINQKEAELNQNQQEEMSTLKKYLLYTDQPEVISTGTTEKEIEEDKRLINAFKSMNRNENWEIINKNQKINSDVRNEQLRFSNINEQQKQKQTQFQEVNENQVENPHQFISFKSDSKFEQVDNSKIEENVKSKYDELPETQLQSKEDMKREKEQKIQEMQKIIEEQLKNKTLKKKSETNKSLLYESEYLQWERITQTKPYPEINFVMTQNNLRKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQ33</accession>
    <name>A0A8S1NQ33_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0900161</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQ33"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="162" mass="19276" checksum="E4034ED5949F1B4F" modified="2022-10-12" version="1">MTSEYSLEWKYMPVTGYLIDQKSNKVIWDLKSEDSKWPKHLFTVSISQQKEFMSDPELGWNWHKSWNQPDINYTINELFEPSITFSGLKFGTTSQNNKHSKFNLIFLLFYSRKEEVLILDSQISSDVFFDSRNRSNHKNGEISQEMFIDLFPLLLIDQILYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NRA2</accession>
    <name>A0A8S1NRA2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-tyrosine ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0830096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NRA2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="51719" checksum="0EB600EB53D9C66E" modified="2022-10-12" version="1">MIQYIYQYHSFSIYLFLLQKINNKCLHSYRYTFKVQLLNIQLKSNVRIANLRLKQLIIQFDSKKFAQTINSNFFISLIFYFTLLCCSWKQQQTYYFSNPRKKKNELQILLSQLIINLNYKSCNLIRNLKQFCQTNKKYVGDIIPLSFIIEINAQENNLNRVLKEFTLAYEQFQPLSFMKTLINYFTKHNSQYFLPKMHHRFLDEENNYIWQLIRFIIKILKPNEFNRGRGIQFFRTIEELKSLLKNFNKGTSEYQFSQGQIKSSSFMEENLIQEFGFQILIHQRYLFLIKVQRDYHQICLIQITEMHLFIQQIMQKLDVIFRIELLTNGEFTKTVLPKIKSIIVLTWMAVQNTFKKCKYSFEIFGYDFMLDEQLKPWLIEINTNPYLEESSQLLKELIPKMIDDAIKILIDPLFTKINESTSLEHLLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NSR3</accession>
    <name>A0A8S1NSR3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0920098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NSR3"/>
    <dbReference type="OMA" id="RDAYIEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="240" mass="28093" checksum="A01ECF78E9A21953" modified="2022-10-12" version="1">MLTIRNEGPFLVQELDVSLQQDNNNAIVIDESDLVILIPDEQLTKKRKQKLSRQNNKKQNKVQSFKKSNTNLISNKKEKKQQKQNNIHCKNKFFKDINMQCSQIIQNNDLNNCPLKFCTNQNNVSNQSKAFQSMMENVQKYKEQVSWSNKKDHIQIPDKLLKDEIQDIQLFVPQIKGVNDSICSNTLYLNKIKDKILHVSSPTQNTQNIKYPKDISRDAYIEYLKNIQMNRVISQNKQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NSZ3</accession>
    <name>A0A8S1NSZ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0990137</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="5">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GcvH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NSZ3"/>
    <dbReference type="OMA" id="EHEWLSG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002270">
        <property type="entry name" value="PRK01202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 5" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="6">
        <location>
            <begin position="32"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="4">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617453-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8095758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="135" mass="15377" checksum="7340588FB90365F9" modified="2022-10-12" version="1">MRSIMKLRQAFSVLTKYTKDHEWIKYDTQTKIAKIGITDYAQKELGDIVHVDFQKVGFVFKTHQSLGAIESVKVAADIYAPVACEIVGINEDLKDSPNLINEKAEETWILQTKVQNEAELDTLLDKSSYDKIINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUH1</accession>
    <name>A0A8S1NUH1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CBF1-interacting co-repressor CIR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0970158</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8095039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NUH1"/>
    <dbReference type="OMA" id="DRVHKSH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019339">
        <property type="entry name" value="CIR_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022209">
        <property type="entry name" value="CWC25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051376">
        <property type="entry name" value="CWC25_splicing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16196">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16196:SF0">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10197">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12542">
        <property type="entry name" value="CWC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01083">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="CBF1-interacting co-repressor CIR N-terminal" evidence="9">
        <location>
            <begin position="10"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="171"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="276"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8095039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="349" mass="41136" checksum="84059B3F289A0A80" modified="2022-10-12" version="1">MGLNYLQKKNWHPGSLKNQEIVWIREQIQADIIKREQERAKKLAEEKNIEELKRIQVEAGLIPKSHLDRMEWMYDVSKLEKQNQNSAEEYLLGKTATSQEVEKLADQREKAKQTQQYQTVFKEETTNAKCEDFVLVHEDPMFQIMKGEQQMKQVLLQNPLKVKQLQEKIEANKKIKKEKSHKDKKDKNKKKKDKKDKKSSKKSKKHNRSSSSSSSSSSSQEHEISTAVSTGSSHSQIYQQYLKERLGNIAVTDEHGNVKADFSLMKKKYRNNLNPQQSHEELRNQMAADGQKRLQQQLKQIEEEPKQQQQQGGGQFLNKIRKEIYNQNDQNAYQDRIKRNRHFQERFNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWM2</accession>
    <name>A0A8S1NWM2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1000021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8095960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="264"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="45918" checksum="C2FB282DDEE6928A" modified="2022-10-12" version="1">MTQDYYKQKIEQLTETLLDQFESSFNKFENYLDSLLNLTDSINSNYQQIAFPSVNVPQTNFENLNFAQQLFKEIQPLIKQNVNNQVKHKKKPIYQEQNLQMLGVKQNMTSEFQKLKIEKIQNQMEQKVSDRDVLLQESNKNKSLLCYQSNNEQFETVYTNNQSNVKSFTYQLVSQYSISQNECCRAIAINKDCSIVLAGCGKEIKVYEFKQGFIKQIQILNEHNNDVHTLNFMKKSNQFISGSYQSIIIWQYNQNNLWVTQQILNGHNSWIYCLILNNNEDLIISGGSDNTIKFWTKNNEWTCQQTIKDHFNSVFGLSLNQQQNKVVSCGNDXDNGKTINQSDKMNILLFQYNNNNISYKSANIFLKQRGLLIRFKKLIIKKQIFIINNNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXG7</accession>
    <name>A0A8S1NXG7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Steroid 5-alpha reductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0960147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NXG7"/>
    <dbReference type="OMA" id="YWVAPYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010721">
        <property type="entry name" value="UstE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06966">
        <property type="entry name" value="DUF1295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="30070" checksum="650EF41A458C410F" modified="2022-10-12" version="1">MFKQHQEFAKVVVNDFLGGPKIIKQCHIINAQKGLTAFFVVFLIWWYQNFSLGAYLYLALHGSYGIIWVVKDFTFPDRTFQNKITIGSSIFTSILLLLYWFIAWLQISEGIPNPSNARVVTAIFAYVFGVVLMIVSDCQKYYTLKYKQGLITEGMFKYNRNPNYTGEMLLYASFAILSGNWLAYSILFTVWIVGFYGFMVNKDISFAKKQGWNEYKKQSYLFLWKIHSNDIINYGLYGALVAYLIWDYQVSGAITLLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXZ1</accession>
    <name>A0A8S1NXZ1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0980079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NXZ1"/>
    <dbReference type="OMA" id="YMVFGDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="373" mass="44311" checksum="CCEED7E585A46229" modified="2022-10-12" version="1">MNVQVENSKIEAIIQWSKELFSLEGQVKRFTAEMNEVVSLCTKEKYELNFVQNTKSKRWIELDIGIKQKVEVYANNELQNIDLIVFTIQIGAQYPVKDVRIVCKTTFVRPTLADGRNLIADVLLQPWNYKLSLVSIIKQIPSFLDRVLLNRFDKIYLQNIGQYYLGSSYSIDELKDYPDLARFPTIQQQNAFFQNIQVRLIGLSDAHFYLFEMIDGKDDYVRLIFRAPLQSCVQLKRKKENSTQLSISWKNYKNKQEEQQTFTINEYDKFIRLFLRRLNQYQHVRMTSNSYMVFGDQQLAEKQKINSIMKNLNQLENEIDKKFNQQTINKLMDLYQQAIEFYSSASDYLYEIYLNKLQTLIQRQDVQVILQYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZN2</accession>
    <name>A0A8S1NZN2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1030097</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZN2"/>
    <dbReference type="OMA" id="MGFLPEK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001817">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8097169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="424" mass="48426" checksum="5C68133D481D42DE" modified="2022-10-12" version="1">MRIEDMITTGTLRSYGDQSPVKKMLHAPTMKLYVVKEEPLHNKEIRKNLKDWISFWQNKLANSNLHVQVYATFWNTPEGYVSIVMEYMNGGSLQNLLESMGFLPEKSIKQLVPSILQGLQKIHQQGAQCHGALGPSQILFLRDGTVKLGQGLQYRVQVQGNQLFNSYILGKPKDVQSLYDPTILETPSLWSKASSEKVHFPAQEDFILERANDIWKLGWLVLNCAIGTLEFHPKAQQIYEGSRIIIEEIAKYTDHLKDTCCLLHSESKVISLVEQNQKTISEPTKITILELLPSDKFSPEFIQFLCSTLKIDPRQRQYTEQLLQHPWLVSGRDPKGPNVQLKELLNISNAWNAFLPEEFQGQGAQKLERLCDALYLVLQNSERPTVNKMQLQENSPIIKELSYDMGLNAKAVSDRIMSIFQSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1S5</accession>
    <name>A0A8S1P1S5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0960108</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1S5"/>
    <dbReference type="OMA" id="AGIINEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="512"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="602"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="638"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="788"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="27"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="274"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8094614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="816" mass="97107" checksum="498B25CFEF826DB1" modified="2022-10-12" version="1">MSQSSPMSVKSSMEGFKLTFDDFLKLDLKQLQINIIGTENEYGFSFTLYKIQVKHPNIQPFICNRRYNEFYALHKVLKMKYCNLDLPQFPSKHEWLFKTEKRKAGFHLFLNKLVLIAMQHPQIQSNILKFLYYFLTRDLLGNQKQTILASTDVQNEDEPDWQFRYHNLSTEDASQVGSYSFDQQKKLATNVQLRFSDDETWQTFYGKIQEGTLQLYQHISYNYFSCQIILFQIKIELADDVILMYHDYDYQKIQLTVNDPNLLKIWYTQLKLASQDVIQYEGMHDDFHSLGKVQIQIKEAINLSNIIQDSFLFVKISFPPFTCQTKQHVYSNNPQWSQDYILPIIDSFSVLRLELISEIQDGILKRKVKQSIIGVAEIQIADLLSYHSGTKFSRISLPLNKDSNNNDVFQFLKKNSTNLDEQQPCLIVTIRNMSSPLSLFGPGPVFSYENEQLEPDLTVIKMNIIRFKRIISLMIQFQLALEDVVDQKYPRLTLIVFTFMFIFIYNADLNNILQYFGFLLLFILIYQHPTIHHYVGLFLDKYILCELNQYYKTPQLFTQEEKDLKKNTQSLDKVIKFEGNEIKLEVDDENEPKESFYQKIRAIKKTFFIVQLYLTYLSNFAEKVINLFTWQEYHRTLWFLYALFSLYCVICILPIRYIMLAGIINEWFDGRIIFKKRQAHNQKIAKVMMEYIYKKHAPELVCIPNYLDVAFPKGDFRTKMANELLMHLTISMNQEETNKYQNGNEVIRDLTTCSQKLKWVSITDQHFVRDQLYYELRQKGKFRMKPHYLVYYIITNFIYNIPSQYYRLRRPIQLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5E0</accession>
    <name>A0A8S1P5E0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ABC transporter family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T1060103</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ITSHYRY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001172">
        <property type="entry name" value="Cystic fibrosis transmembrane conductance regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000330">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000331">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004122">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF330">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="295"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="337"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="733"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="793"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="819"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="878"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="977"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1004"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="176"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="425"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="793"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1081"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="694"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="701"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8098206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1341" mass="155539" checksum="1B62FC33163A2E77" modified="2022-10-12" version="1">MEQSFLNKQVKSDNWLFSDITPLINTINGMDGQIQNNDLKKFVIKESDCEQILQELKDILQSKPKYLYRKIFKHFFLFRTFQLLGIQTATDMIMLGNLLMLNYATDSLINLNRELLYQPILSICGIICLSLLKTYFNAKTDQFVNLFVLRLRLISEYLIYDKALRVQCISNKGDSDSHSANINTLLTADLDQIMLINFTIRDTWEAILLITGCLIFLYHLDSHAGTIVVITMFCAQVFNLIVTAVMMRSEKKMLEYKDMRVGLSTDVIEGMKQIKYLSWEETFSKKIMGFRNWEFFMLSLLKGFDGLCSIFWNNVSYVLLCTYIISSVNNGKSLSDLNIFSLIAVFNTMIFPLGILPWCINSGLLAYVSFKRISQFLKQQEIDPDDRFQLPYVKEGDVVLIEGLLCEWPIEKNEQQKRAQKKNQISDDVLVFDQVNENSQFILRIDELKIERNTLNFIIGKIGSGKSALFNAILNELQKYNQYEGMYNSNSIRSDSLISGQRMLTISDQIIIKGNIGYCSQTSWVQNMSIRDNILFGLPFNQKLYNEVIDVCEIRKDIMTFEKQDFHEIGPDGKNLSGGQKQRITLARALYQNCDIYLFDDIFSSLDIHIADQIFQKTILDFLINNGKTVLLITSHYRYLNQVPPNVKSRIIYLENGQIINDKNIINEYLEKEQEPEEDKPNIEEELEEMIPELTKQKSSKKSEKEQFLHKVEDDDTENQEQREQGKINMNTWITYFSSMSWFLLIGWVIVNFFMQGSMSLIDFWLKSELKGNEDTWLHQITIYFNNSFTDTLLYLTLFALLVTFVRAALYVSTALRSAWVLFVKLNKVLMQSVMKFYDKTNAGRIINRIQDDTQMIDDDLSWTFHCFLENVSRVTGLSIGLVILEPIFVLILIGVVVLYYQVSKIYIKSNREIKRLKSVNQGSLLQTVNETLTGIKIIRAFDKNSLFCQTFQNKLQNWVMTDQCGERSKLWFGVRLNLMSNLILIFVSSFVILTIIFDFSDDYTVQALAITYSMVVLDSFYDLFSLYLRMESGIVSVERVKQYFSNETENIDKVQILPKIDFDIWKDVKEFTDFRIENAVTFKNIYLTYENVSAEAKFALKNFSLTIKKGQKIAFVGRTGSGKTSILNILFGLYHHQYGQIFINGMDVIIYIYIYLYQSSKLTLRELRSQLSVVPQFGFLYNASVKDNLDPQNKTTKDVIEKLFIDTGFQLRGINNTNNNSCNADFIISQNGSNLSNGEKQVLNFLRIVLMNKDIICLDEATSNMDPKTDQLLHDLLFKFAENRTLLVITHRLENIKIYDKIVVMEQGEIVEQGEYQELIKIEGGFFNKLMSHHQKQIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6N0</accession>
    <name>A0A8S1P6N0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1070064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6N0"/>
    <dbReference type="OMA" id="MIVGAKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="141" mass="16145" checksum="6A077758BCCA92F8" modified="2022-10-12" version="1">MVELDAKKQLAISNLALLMIEIVLLSEYSYMNEHPHRNESLTYVRFAISGQVFMMLAFMGLIATYFFPHRYVKLGILGSLCLGFVLVFIGMIVGAKYYKDGYDTIASTWWIEVGLIILGLLNYIQAFSNEPKANEEYQRQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PDC0</accession>
    <name>A0A8S1PDC0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1140021</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8100924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PDC0"/>
    <dbReference type="OMA" id="TMGIGRT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="232"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="522"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="591"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="742"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="886"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="306"/>
            <end position="313"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8100924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="959" mass="111316" checksum="31ED5C3ABEB7600B" modified="2022-10-12" version="1">MFNSKVDENFKQVKMNPLNNSTSKQLYSFSKSPRFNYKVNVDQNSSSFYEIKGTIGSPNKGVSFGLGTKVDFSKLSDKTPGPGSYKLEKEKTSTYLQKHTMGIGRTYFKQKNDMPSVGKYNIASSIIKNERIPHFGQKIAMKDETCTPGPGKYDIQYKEHSQSVIYHPVSGRTSRTPDYIPGPQQYRPKTELSASYVNSNWSNCNVPKFSKEERFNSKQSSTPGPGKYQIPGEFVYLRVRPIDYDQSMLSIDQNVISIKDPTNKQAEWNQYRYDKIFPSSSTQQELFESVFSFDYENKNGCIISYGQSGSGKSYSLFGNIQNPGIVPLLIQQLLQKGQNVQVSFQEIYVDQIKDLNTNLITEEFTKRQILLINDFWEMIKILRTTDIKRQMRTHIILTLEINQNTKLQFVDLAGSERVAKNITEGEKFQEAILITASHQVLNRCLNSFNQNPNKILPKKESKLTSALLIDNNTQVVLIGAINPSQSNYEECLLTLQYLDRTKNIQVTIKKQQSMLGFDNQVNIQQEKEIKKLKDEIEEYKIKIEQLNADRKKRFLELQRLLGLDIDLERLSAKNAKDITIFKNQQEALLKNVSLQQQIDEYHYENAQLKKEIEDLKKDTHNKLERYQQQILEQKEINKKLKDQLQLSKMSGDDAIRLLSQDRDQFIKKLQDESKNLLEDKVASILNLPQTAQTKNVENQKLQELKKLIKTEVEKEFNKSLEIIKTEYNKSLDHYKFQYEQKLNAKVEEIESFLNQFKKYREKKKSQINEIVEELLDLYDIITKQGKVIDKIETGGYSGGLKSFSIPKQDKPNLPNKVKHKNLFHFLETKSVTATLTKKASNVEKTIKTATKQLRQSQSQIILEIDYNQLDSINFMSMDFSTLRTYANKLREMIKELQDQITVNSDKFKQQIAQLQRERDDAQQKYNMESRKYNQTRVVIESQNRILSKVRPLSSVSRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PDD5</accession>
    <name>A0A8S1PDD5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1140031</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8100944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PDD5"/>
    <dbReference type="OMA" id="PLIKVYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06606">
        <property type="entry name" value="STKc_MAPKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001222">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002032">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF369">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="79"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="336"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8100944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="402" mass="45880" checksum="A8923CBC4EBD79DB" modified="2022-10-12" version="1">MGCCMKRKAHVAASSNNPSLRQTKKFDTQRSRIDEMKSEVFEKTLYGNEDNYYDADELRCIDRNQLLSLIKSEPKKIRWTPGQVIGQGSFGRVIEAMNLDTGQLMAVKQVMVDVRNEDRIIALEIEIDLLSLINHKNIVSYYGMERTEKTLNIFLERVAGGSLSSMLQKFGPFKEPLIKVYMRQILLGLEYLHQNGIMHRDIKGANVLVDNQGVCKLADFGSSKKIALNSNSTIFGTPNFMAPEVVKQQKSGRKADIWSLGCTMIELATGKPPWHQITNQFAVMIRIGKGEIPEIPEGFSEEAKNFVSHCLEVDEHKRWNATKLLKHPFLIQQNKLEIPQGRTPLRNTPGSKSKQKQKSFKYPEQETSHSPGQLKEFDQQQSDHLQPMIDSSFVLKQRRQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHQ2</accession>
    <name>A0A8S1PHQ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1170038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8101928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHQ2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="11520" checksum="07C4B4F0749F5C50" modified="2022-10-12" version="1">MFLTQLRLWIYKNKKTLPQNTQLLIHAKVYQLWMMMDLNYLRVMLFLDTLLINISCMIFILKTHKKLPKQNVIQIGIIQAQNQFPNLCLNTQLDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIQ4</accession>
    <name>A0A8S1PIQ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1190072</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPRE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIQ4"/>
    <dbReference type="OMA" id="RRETAVC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000028">
        <property type="entry name" value="RP/EB family microtubule-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="10">
        <location>
            <begin position="5"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8102771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="242" mass="28559" checksum="78B37223A069179B" modified="2022-10-12" version="1">MKSHNQGRQDFLQWINELTECDYPKVELLSDGIGYCQIIDALHPGAIYLSKLNFMARFPDEYTKNLKVLDDAFSKLKIDKVVPIDKLSKCKFQDNMTFLQWMYNYASKVNPFVKNYRGYQRRLEAFEKQHHGRYTQMSAHLIPNTEFLKFKQTDIDGRTFLKVESTKAQQAEEAIKELEIDIKNKMDYNWKLIYALDDLQYQRDVLYGLLTKIDQCVQKSNDPAAIKMHNVIMEEPIDFSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKI5</accession>
    <name>A0A8S1PKI5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1220018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKI5"/>
    <dbReference type="OMA" id="FIAENIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1170" mass="139401" checksum="14C7DF444E6435E0" modified="2022-10-12" version="1">MDVWRQIGNQKVLDVETVLEAFNNTNSPDQIAYSNCLGFMKLLKSSDLLEQIKNLYSDRYFCQQLSQSQFDNFLRITIKSISLYQGRDEDFVDGINLLQDYFLNFNTNQFCFALLSNKKLLEKHPKIMLNIFSKAIIQEDQILLNKIIQLLGQKQLKSFHHQFLIDLIQIDLTQKQSFRTYAEKLFDSFIGNSEQQLKITNLTFQDIILAFKRLIKWDSLNYKFIAIKLDHYIMNQDKNNDVNVLKLIFYIMTSQQSHLWINCSYLTKTIKLQWKTADYEFRFYRVLKLLRINNHRDVDSSYRMGFNGLSGQKVDSFRLAQDLEQNIKKTIQESDHQIVYLQFIKQAFKREPYCFSYSFIQFLLQYRYEDKPDLYQPFWSLVSKIYSFFFIQLYEQDITNVFDFQGMFVDENGNTKLTTKIGIQKKNARNLVPYFQQLIINSQNLIIMVYKKFYKLFLSEKQIFNFFTNLIYEPLYQQIEFQFPDKSNLKQNQNTQLYYLFSEILDNFQNTKNILQDYLTEIYKQLNSNSKENTFRILSYYQKIHKKYQQSSLSDYCLDLINKSHSFIISEIFQGKNELSQQINITQSQNLKNNLQQLLQLFQPLSDVRCKELKSIQDIILTNFQVNYNISIDTSTSRCFLICLLKVNNKLSQQYYEKQVNNLLQQFENLFLSNGYINQEKVEQQCQKFSDFDEKDQDGQYYQSIIEQKSIAVEQQYIRSIPQLKQQFQILLYLQLLNLLIQRINRYEFEVEGLITSLRRILTHPYVTDDFIIDIWNEIYLIIEQLNEQSSEKWLELTQQKLMNNVLIHLDQTISITTTLILAVILNQKSLQTKQIRIQKLFFLVSELKSNNQKNSLYRIIFNNLDKLDSNLYQKIFFEMKDQLMIQGGTYKLLKLILHEPDNIDHFNLIIKVFLGQEIHQIQIPILQFIAENIFKIGVRYQILIYLGVCGRSLKTKKFAKHLIKQFQLALLQYQDLTQFIIVLNSVVYLVSLLIKDLNYQSTYWLFKKTLKHFFQPRIKQNNKKCSQIINLISYFIEQSVYLTPIKSEYEFSLSDKLEINIKKLPELIQQSNKSITDFTDIISILQQYLNDVISFGKKNVENLKKDQQPQSNLDIFDALLFSMNRNQSKQILKSKSQQLSNPFQNFIDFKQSSKMEKVTKALDIDKEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PL96</accession>
    <name>A0A8S1PL96_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1220139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PL96"/>
    <dbReference type="OMA" id="FKFFGKA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="157" mass="18053" checksum="2ADA506EF6D1B18D" modified="2022-10-12" version="1">MNHEAENAPLIQEEFPVQQSHVSVQEQPKVVSINKLFLQNTAQAFSPGMMISKQIYVFSRTLKKSIEQYRNNERIQFVKNVSSSLEEFDGVSYSNDALNQDQSITAKGFKFFGKAISKIRDTIRGTQSQQEPQGNQQQNRNESLFPEQSQRIDEEHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLX3</accession>
    <name>A0A8S1PLX3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleoplasmin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1210186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLX3"/>
    <dbReference type="OMA" id="LFKLIHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoplasmin-like" evidence="2">
        <location>
            <begin position="2"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="244"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8103713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="341" mass="39765" checksum="829237FDCCB599E8" modified="2022-10-12" version="1">MFWGTQVTQGQPYKLTAEEATVIHISNVVLAEGDSKVQVYAKVKGKEFVLANLEKNKLEQAFLDLYFRVDQQVEIGIKGKGSVHLSGYIEPDEEELNEDLDFEDDESEEVEQQKVKKPEPPKQTEQVKQQPKEQQKPQTQQQQKTQVQQQKPQNQQQKAQPEQQQKAKPQATTQKQQQQQPKKADEDWNDNEDGEDEEEELLDQSDVDDEEVEGDLGDEEDDDEEDVDDDEDDDEPEQNKKFIKGNQGQQVKPQQKQQQKPQHQQQQYNKQQHQGNQQFKQGQKPFQQQHKQGQQYHQNKGGNQNQQKNFKKNNNFNNNNNNRKNFNKKNNNRGGFKGGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMS0</accession>
    <name>A0A8S1PMS0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1230107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMS0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="232" mass="27626" checksum="0ABEC4C9EAAC2A15" modified="2022-10-12" version="1">MVYKIRNKSFFWTRAGWKNNWHPKNFNAPRPSSSEFTIGIRCRYDHNSFLRAYHSYRKISRHCKQYFFGNKELEELFQMGLRTFFIVPHIAECQVTQIKHGGERRMVDQIDRDFELVSYNSHPYQLFTYTIWNQYLANQQEAYEQRKNGGKAIEDQVIDHISELVKEEKSKLGPGKQLSIERTADIVMNVMRQLRAAQQRPNLNNRRADGEFDDFLEQRRPFTAPNNQSATH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PNW1</accession>
    <name>A0A8S1PNW1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1240017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PNW1"/>
    <dbReference type="OMA" id="MINDIEG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737:SF6">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VIT" evidence="2">
        <location>
            <begin position="31"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="340"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8104459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="876" mass="101738" checksum="0EF2A97ACC6B3BA8" modified="2022-10-12" version="1">MIDDQFYQPKIINNSLLQRGIKNEDKIISASYIQVCYDVQRNTIIPLKNVNYHLTILQGLSVVELQQIYSTENYDYPLELEYVFSINENAAVTKMKVELGEKVVYGIIKEKEEAEIEYNNGMNQGKTMVYSEEDKEFSQIKRVRIGQLAPKQELKIIFQYVQPLDVFLNKFWKIDINPIIDNNYLHQKDQSQNQNNQIINYLNSKFNLKDFKFLYKQDIQITFDTGSHITFWKSPTHKLHSTDIDDAKQDGKNNKIILRLDNIPENQNPEKQFTLLFSSDDINLPRAILSHTNNDALEYQKYCATLTFIPKFNQVSLDDAYTQYLDSLSLPYNQVLNRGNYLFFIDRSGSMNGQRIKRAKESLILFLKSLPQDSYFNIISFGNKYISIWNESKQYNQETLDEAIKCVDNMNANMGGTDIYTSLKEMVYNSSYGTSNETILNVFLLTDGEDTARPIIELVQKNNRAETRIYTLGIGSRCNQYLIKRMAEVGNGKFHFVSDNEEINAKVIDLLEDSLTPYLKAFKVETNAIKISSIIPDPESIVSLKKNQELTIQILFSSQQDQDNIQFKINCFDSQDQKQINYNVNLNLNQSKDNEYFHKLAAHKFITYYENALKYGEQQVNFIKLNKQYLDDKDIVNLSVENQILSNKTAFVCEICELEDQFKQQARSKITIQIPQRPKRHFQDQLQWCIGMQQCMVPPQCGSSQIMNMPLPAMGSKSNAMVLPMSQQIQYKCCTTGLPISMFSPMGNLRQEQCAQMKDFPMQTNTQLQLQKLSDIKKDELNVKLSYNQLIQFAQADGSFKISNDIQLKINFNNLNNEQGLKDDVWNTLLILLYLEKYCSEDKQSWQLVFSKSILYLSKHGINYTEKKLEYEHSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQE6</accession>
    <name>A0A8S1PQE6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1250048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQE6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="89" mass="10771" checksum="201D7CBC79552E3E" modified="2022-10-12" version="1">MRLILILQKLFLNFIPDDRLFVEYARTESQLNYGEPFNMTVVTQLVCVLAIDFRKVIQRQNKNFQTLRSSLIYYNRFRQRTLIYQTNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRI0</accession>
    <name>A0A8S1PRI0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RIIa domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1260085</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ropporin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRI0"/>
    <dbReference type="OMA" id="CKPNDIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22985">
        <property type="entry name" value="DD_CrRSP11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003117">
        <property type="entry name" value="cAMP_dep_PK_reg_su_I/II_a/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14952:SF9">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14952">
        <property type="entry name" value="ROPPORIN-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02197">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00394">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RIIa" evidence="5">
        <location>
            <begin position="19"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8105158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="67" mass="7930" checksum="D1A2D12828C2976D" modified="2022-10-12" version="1">MADVREQRIYCAEQIVVPPELPVILKHYAKEVIRNKPGDIVDFSAKYFRSLLEKRTKEHEFSEIVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PV12</accession>
    <name>A0A8S1PV12_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1320042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8106892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PV12"/>
    <dbReference type="OMA" id="VMIDCYR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="364" mass="42627" checksum="607A1F7D0ABCDD41" modified="2022-10-12" version="1">MQDQQSMFSVYSNPWNKQNITKEKGYLLAKSIDKHPEFKTEILDAIGQQQQQTRNIFKDPVSMQLCKYDSTIGPKLENNKIVIRSIVGKPDIFKRKLSLLTGQDPKSSSRRTFVKGNTTNTFRNNNNTIKTQQVYTGEELLAKVNLIEKHLFESRLKEDQEIMNMPFQKKQILLKETRALEEYLKLEKEQNNLLIGVTKQSNKDPSKSVMIDCYRFRRKQEYCNTNDSLHRNNEDKTFKILEIDDDSGYPLFMMSQNDEVIRKPSIINQDETTQSILKQLNDEPVYRSFSSKDYLKSQSNRYTKIASDKINMNIDNLIVQGQSKLDQEIKFDINAKYLLQPIDKGNKEETITENYSGYYKINFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXZ5</accession>
    <name>A0A8S1PXZ5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1350086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXZ5"/>
    <dbReference type="OMA" id="EHINFEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF89">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="1">
        <location>
            <begin position="40"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="344" mass="42013" checksum="C6E280EA89E2A861" modified="2022-10-12" version="1">MNQPQFQALPSYDNLDMIRKTFPPLEHINFDHEFKNQEKSKYFIIRTQGEDNVHRAMKYGIWTSSSRKNERLNEAFSQGDVYLFFTEINSLCFSGMAKLTSGFDPKSHFKYWLIENKWFGLFQIKWLYVKDLPFKLFENIKQIQKFEGSDETIKSVYDLIDCTELTLENGIKMVEIFKKEESKKSLFEDFSQLDKLENQSRQQRDSNPNFEKKFEELRAVFETIPYSFSAASYERRQQRKPQYGYYNYGYYQQPQYWQSNSGFYQQEQYQQYPYQQYQNKYQDQTDQKFNKKETQQPGNLEDKFEMQQNRKYKYDKKKRFNNRNNQQIEYVQKYQQETIVQQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYL8</accession>
    <name>A0A8S1PYL8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1340101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYL8"/>
    <dbReference type="OMA" id="WFFIRII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="275" mass="33799" checksum="012CF43535243617" modified="2022-10-12" version="1">MRRTMEVEEQNFEAEEWGIAQQMQRILGEIHYLMQEQILAIDYYSSQRRNYYQVKDNLEKKEDSVNARLENYKQLHQQYIGVKQIYHQILLFTKDKKLLNQRIQKVGYKEQHFLIENPQNYEIEEVKDPKYQKDKENQIIYFRKIDYKWFFIRIIKNYEFTKYLNATQILDIEAFLCDGSQKMEKQDHQHELEIMKQVNQWFKQLIFNVQYRKIFRKQLTEIIQYIEETIKILFKGHKCSTCNQVFLQTEDGKFVQPCVKLNENDNLHHYSCLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYR5</accession>
    <name>A0A8S1PYR5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1370012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYR5"/>
    <dbReference type="OMA" id="VVCAKVS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016641">
        <property type="entry name" value="EGD2/NACA0like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21713">
        <property type="entry name" value="NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="620"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="689"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="275"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="124"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="648"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="728"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="743"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8108346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="835" mass="99468" checksum="53654C33CE52EE18" modified="2022-10-12" version="1">MQQRARSLIARQYYKNKYGPQLIEDDNVLDVQIGDDNTLQARQGLVITINSDDEGPKQKPSLPNFSETVVKKQECALQKFEDRNSTSQLQLTLQQQLQTQTDVYKQQQHQVTNTLDSMSQLQKSLSQIKIQQPQPPQNVNNQQQQNQQQKQQQVRQPITKGIGDQNQKNNEKPKQRVISIPDNIQTKMNIESDEEEVQMNKIPRKQVRPNEDETLPQIHFKKKADVFSQLKQKITQPLNENTALQIQYKYKQRDEYSPDVEPPQNVAPVSYQDFIAMKNREINAAKQRKQDEEYRKQLLQQKKQEKVAPEKIREGETLQQYQQRMIEYLHKQNKYKNKKKYKRGTDLDNKKTAEEKQKISELMKMIDPNFQQEDIEEKPIKPDLLNYDVLLKNGLVLKQAFVKPPEPDINPMLNVYKRGRYATEFIFSGGIDLDDGPYGQQSRKKPKKLPFPKHRLSLSPWEYYGVGPEEYFATRNPNFEFKQKYVPVKDYYNEMPRPKQNPKYFSYDVDQLLAKRYQTIHEEYQYQTSFIGYKQRHWTPASHLKAVCPSLVLEYEKYERDRLFCYIVCAKVSKYQLIETVGILTSKYTKNKIENLVMTYWKGDSKGEKKTISNLEKQIPQEQMKQTDEMELFKDDDEEENEKKKKISKEQRHLEDEWDPNKDEATERGKRNKLRKEKFQRIAEELKLKQQIQPEKIKKKSKKDIEGDIMEVENQTKDENQQSQSKKEQKKQKRSKKKKQEQSKQIVEQEANQFEQQFQQEQQNNNEVQSKQESEEQVFYDNEDLVFKAKMIEFNQKRFNFTGTDVDLQESMKLVFPNNQQQKLDSERYKQFMQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYX3</accession>
    <name>A0A8S1PYX3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1370054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYX3"/>
    <dbReference type="OMA" id="VRLTGHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035745225" evidence="2">
        <location>
            <begin position="21"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8108426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="516" mass="61324" checksum="522C47CCEDE7EA5F" modified="2022-10-12" version="1">MLIILTSIILLLSLLTKQISQILQESSEMIFDLYSKRQKDIAKQTLEIFWVTYIHSVSQPLISLHRLYNFQQRLNNLKVNSQEVNENCYNQPLIEIEKPCICYLYKSFINSNQNDPKNMLKNLMQFQTALPTHIFGVQQYMGFFEDSQEQYLFNYPCLDFRNYKLYDPKTRPWYIEAEKAYNQSNFQQYSVRLTGHYLTIPYNDYRFSITLPLINFQKNMMGTLRVHIQSKILQDTIYKDSNKTNLILTTNDGMLLISNLFNNSGFELGTDYFYNETKTGFNLEDWQAITQKNLSNCEHVDIGFTCRQNKIDKKDYYMSHTTLEQYNLKIILYQTKFEYQQYLQEHQKQMITQIQEGLIKGVVFLIVIAIVISIISSLFLGKMLKPLQQIAKIFQITKHFSSQSITQRFLKFKISNNYAGIFKKPGCFISKDVFLFCQCIEQFVINFSTVKKKSNSIYERFDQIKYPQKNEKDQIDFLDYFQQQILNKKFQELDINQKQIRNLIKTCLVSSTRKEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1A4</accession>
    <name>A0A8S1Q1A4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1400012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1A4"/>
    <dbReference type="OMA" id="DIKECEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="15377" checksum="76F7EE471EA7D876" modified="2022-10-12" version="1">MSQDEDELFSAKSEILFNVKQCEELFQKDKSEKLKQDIETFKKNIKTAKEKEEEFYKTLLLEEKEQESIFGFPGYQKKEEPKVIVWDPLEGNLIITEHEFKKKYDQPRQKNSNRQSQIPLQPEIYINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q245</accession>
    <name>A0A8S1Q245_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1400030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="343" mass="41257" checksum="FD8B5A7DAC3BA7C0" modified="2022-10-12" version="1">MNKKLANTYNQISDVFSRAQIKNAVESLDNYHDRLKLIDSIIDRRQQIRQANDLSKITKYAHIFRKMIQRYRNKKEDQAANQRLQMQMVMSMFAPKGKQSSPRHLYKNLEQRKIDPQQLKIKEISEQRRDAVQLKMNRTSKISNFHSDRKYNNQRNNTKEGCRFSNRTQRFLSSIDVDDQYQEKELYLWSVDDKEIKSLQSKIKQSVILKTRINLNQNTKDNENVLKLLQKQQSTLSRRQYKPYSQPQLQDXNYTFSLSATANLLACQIQSLLLYSMSFSKQKIWRSFECIITFRLLLNQYRFSFKYIEHNNIIITKIKCQYIDNSIFCKFYHVIRLFYIQHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4S4</accession>
    <name>A0A8S1Q4S4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial import inner membrane translocase subunit TIM22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1410103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="12231" checksum="70FF49B690D0BA17" modified="2022-10-12" version="1" fragment="single">MTQEPNPFKHVTFMDTNCAAKAGMVTVAGGAMGFLMALFMNAVEMRDMDYGRTKQSTRYVLRIQIKCLEWQKDLLFLVLFILYLNVNQKNQELEMMQQIHFFLAX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q6R3</accession>
    <name>A0A8S1Q6R3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T1450091</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q6R3"/>
    <dbReference type="OMA" id="QQYLVFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20336">
        <property type="entry name" value="Rcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000064">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000051">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="459"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="186"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="190"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8110795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="499" mass="58263" checksum="73ADF59050F15576" modified="2022-10-12" version="1">MRNVKMTRSKSEQWLQNQKLNTIKEQGNQVLLNSNQRDHFIIQMSKSDQLGEIINYHSYQEVSQNEEAIKESLSENQNKDLDLARNSVLQIVEQERGQNTQDAINNNEVQIKMQNTPDVANEIPQGNVQGELALINFDQNSNEDDNNDRIESQPKIFSFSIFSQEQFNNNINNNQLEEQQIQQFDQYTMCPICSSNFEKLVQLLECEHQFCLSCYKEYLEDKIKIAKINNILCLQEGCKVIFSEDIIKQIVNEQRFQQYLVFKRKYEIENDPNKKWCPAIGCDRFIEKDPSTNITQCQCGQLICFTCGQIAHQGIQCEDAIQYDFKQALTKYSIKYCPKCKSHIQKNAGCNHMTCACSFEFCWICSQPYHPNHYKYWSIWGCAIWSNGRFKTSKIVDNPDKMRKIYFAPRLVLFILRGPLLIIKLILKAVCKSIVKPFVGLNKKLYQSKKPKFCLSKCIYFLLAELVILLIIILIFPFQFVFYRLGKEISKFTSKGCAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9F5</accession>
    <name>A0A8S1Q9F5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1470056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9F5"/>
    <dbReference type="OMA" id="VIYEYDE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="190" mass="23056" checksum="BA2C5012FDF7EA56" modified="2022-10-12" version="1">MSKDNQKIQLQMSNKYNQIKKIKKIIDDQEQWFEEIFEYYEEVIYEYDEQDPDELSLGSQMDLEHLIENYDIPEDCYEEIVSIYEYYDVSEETEKIDSNKKSNQKTENENNFQVFNLGEEEFIALVDDHKEILDDDILTMKKPIEQEEDEEVNQKHQKKENPINKKSNNKKIIKNKNKKKKVKKQKKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QAV6</accession>
    <name>A0A8S1QAV6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1530012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QAV6"/>
    <dbReference type="OMA" id="QNYLHEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8112826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="530" mass="61849" checksum="C100444741BDC369" modified="2022-10-12" version="1">MILNQLKNQPKWYLKKDWKIKTQILIASLIVYLFIFTILTIAVLVSQNYLHEMLTLFSHQIFLKQTKNSLEYQWVYKRGLELTLLHTAQQISFVRDFNQLFDSLLINNSFKVKEYPMMCLNDPKQNDTYCYTSSVPCGLFGNPINSLQNLGVDSILQTSSVLTSYKFTLDSAVPIYYFNNNNSSQLYCITIGLQFPKTFSPIKRQYYLDHLKASANATDPKATYIVNPYTVITNVINFPMTTNLLDQANGVMGIIVKGIDLNYEALSKFNSNDTKILIIDKTGKVLLSDLYNIKSQPLYHLNESEFSGFNNSDLEQIFQHHYQKEFESNCKNIISTNILCRFNSLSRDNLIIETKNISNSPFIMVMLLKTNFILKQEAEFLEILDSIFRTDIRNSLILSLVIPIFILFCSQFLINIILKQLNRIIYDIKAFLFSNKKEYLTTQLFQKNSMILSETIIDFQAAIINLFQRQATFRKSDECLSVENIQFPIYTRLYKFLVQKLLYIIKQKRFKNNSVTDSFQFFAMKEVFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QB17</accession>
    <name>A0A8S1QB17_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">VLIG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1530001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030383">
        <property type="entry name" value="G_VLIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056861">
        <property type="entry name" value="HMCN1-like_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22796">
        <property type="entry name" value="URG4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22796:SF1">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25106">
        <property type="entry name" value="VWA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51717">
        <property type="entry name" value="G_VLIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="VLIG-type G" evidence="6">
        <location>
            <begin position="795"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="5">
        <location>
            <begin position="2630"/>
            <end position="2844"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1172"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1272"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1438"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="2005"/>
            <end position="2101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8112808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2845" mass="334761" checksum="87C5B6ABFBCAE9C6" modified="2022-10-12" version="1">MNQHNKNIQINLLYSIVTDQAQEFVLDKINFHFKNIQQLSIDSFDEKILNIKGLFGCQKDIEKEIKTEIHQEIDMTNFLKESEKGNLILIQYQDLRLFCLILPIKFFLSKPIQEKSIEIVLFRILHDFTDNIKLCLDDQIFSDWYSGDIVPFSARILERQDYDFREDVNLQVQNYQCQFHNIPQDISKHHIITSNSFQPYIILLKQKEMMNQEEFHQNLTKYVTFTESLMMQILVNQNNQIIWLLDLFQKEHQTQKLREILNGVSTQLNIKITEERKKLIQDLSKKLKSFFNFSKNDHYQHQAEKILINAFSKLKFQSHQFEFECEKDDHKKLIYQALTQILVALKDIQEKFRSKLKQIFQELTQQTTYFVERVIFKIYRQNENQADAILSIWPHLDEDQKFQLDTSIFKSQTQKLEIKEVFSTKQKGTIVVVQTEIEAYILFQQFQSPSFKIIRNLHPDHIKQAIYYYDHNLGYLYIFNYKVSSVQQIIISQTGAIQVDQQFCFQQDQQNQNFLIQQVAYISVQKKFLVLSDKNKILALFDKQQQFISFKCLEEIKVEQRKELKEADFIPSIESKHSYNQLLSCPSGKYFYLANHHCCDRYDYMGKRVECIKIRGQIKIFSDSSDVIILHECNNLTESKRITIIQNLIQQKKFNKQQNNEQIIIGNPALDIVKGSFVKFGPNSQFLSKHKSKNIQLNVESHHYNKIEKYYNNMNLNNVFLSSQQTQFSKCDSNQIKNIIYSRVPQQFCTIENGTLIPLNDGFRQQSMIQNGIKVDQKVKQLHLGFLEDYLSCEEQKIFVVGIIGKQSSGKSYLLNRIFGTRFSVSSARCTDGVWGTMAYVEDQKFLILDCEGLFNGARTEKEEIKMLAFLTAISDITILNSDSTFSRYQNELFNNLVEASKQLNDEKLFKGFLYIVIRDVSTSDNLGLEQELLKNLDRLKTADQEEIVFLNKLFNNKLSVEKMVNNENTLFDQQVKKVRDYIINQSLDSNRWKNGKELIAIVKIVLCQLELSDTSNASLINLQIRIEQIYFESQILWYQFSQNLPSNGELKLNQANYTFEMDDDEYLKQNTEFLQKLYTDLKLNDNILNHKSNLNEANSQFNKMMEQRKQFIIQHTLAEISDIQMVEMQTLIQKENTKLQDFLQLQIDKYQFCQIKCHECCLSCKHFKNHIQYSENLRNIIENEIELLEEQCKNSKIKSQEEEEMIRNSLIRIEKNIEEIENTLKQINNLIKYSTITDLQSSLIIQLKNGNYFKVESFEVNGIKLISENGIKQITKDIEGIYQEYDNIKRQLQSQNEQQANNKIEIENNQNQQQTFLRDSENIENNIKKVNIAKQQLQQKIEALTNQQNTIKYKIQQIQSEIDQINRNLNENELVQQADIDEYQNEKNQIQEELNQLQTRLLQNKTESNFFEQMSLSEFINYFNSQLDKKQAHSLELYKIKDRKKELQAQKDKYQQMMEEKKSLQQKSKVLNRKKIQELERELQDFDLLNLEKQAEFCSKEEQILMKTIYEDFYNLDLYNECHEKAEENELQLEDCLKQNQQLELKQNNDQQINYQEIENLNDINQTNESKIEPEFSQNQQSIQEQENNSCIEVEKQLEISEQIQNNDQQVNTNKIENLNDINQTNESKIEPEFSQNQQSIQEQENNSCIEVEKQLEISEQIQNNDQQVNTNKIENLNDINQTNESKIEPEFSQNQQSIQEQENNSCIEVEKQLEISEQIQNNDQQVNTNKIENLNDINQTNESKIEPEFSQNQQSIQEQENNSCIEVEKQLEISEQIQNNDQQVNTNKIENLNDINQTNESKIEPEFSQNQQSIQEQENNSCIEVEKQLEISEQIQNNDQQVNTNKIENLNDINQTNESKIEPEFSQNQQSIQEQENNSCIEVEKQLEISEQIQNNDQQVNTNKIENLNDINQTNESKIEPEFSQNQQSIQEQENNSCIEVEKQLEISEQIQNNDQQVNTNKIENLNDINQTNESKIEPEFSQTQQQQKEQEIKGLKIKSMFLLLKKQEFEEYQLEIQELSNSYQDLNQKIEKFQTIIQKQQTLLKLANQQNLFQSEKNAVDSQTQDLNSQLRNLEEEVNILRKRLIEIQSQLDSLQIRKSNFMDENNKIVQQIEIQNEKIIRLKNSEKLLKEIWKTGQEQIPLYSQLKDWKEINKFSKFSLQDLQKEKLNIEKQRDNFVTELQEKLNSLEIFNKVGTDAVRLKKLQCKLQELNSLNLNNHSCQRVNHKCDQICKVCPDKICDKEAGHSEFQEHLCSLKQHKCNHICEIPECKQSCSLLFDHPKQHNCAGEHPCKQNCQFCSKKCKSDRSMFHQNHQCEDIYCTETCMLCNKKCNQNHSHSQLCSTFCKKQHEHSKQKSNHLCGEQHECKQLCQDKGVCQIIYCQQERTWQSKFKYIQYVPEAHEKKKCCLIIKPGELEHEKNHTCMIETEKQFHICDQRCPECNKFCEKKYNHPGIHSTQIHINKENQVFTAKEGQLGKIEIKDSNVTLRQYQVGDKSTPETCDQSCKRNGRSHYHLVECKGGSECLGKKSDIKARHSDEKYQGFNHLNFDEVLCLDYWNLKKWGHPIPNEISNISQCNYYCPLCKKINGENSFCLKEAWHTQDNKISSHSFHCAQMHKEQTISGIKIAFVIDTTGSMGSYINNCKEIIKSIIQKAKSHKTIDNSEIEMKFAIVSYKDHDPPYNQEQNVVEMCDFTSENDAIKFLNNLTASGGGDTPEAVLDGLYSSSKLNWSGNFQNLLYLIADAPPHGKEYHEFTDSFPEGCPCKKQQNEIFTHLKNAKIQFKIMKLNQSIEGMITKFKQNYPELSIIAPDSKDDVKFQNLIVGDVCDYLTHNEITYQMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QHD5</accession>
    <name>A0A8S1QHD5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1610062</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QHD5"/>
    <dbReference type="OMA" id="NISDMQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052438">
        <property type="entry name" value="Chromatin_remod/trans_coact"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572">
        <property type="entry name" value="GLIOMA TUMOR SUPPRESSOR CANDIDATE REGION GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572:SF0">
        <property type="entry name" value="GLUTAMINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="431"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="476"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8114766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1218" mass="142510" checksum="9C9D58FE5E889C01" modified="2022-10-12" version="1">MFPKQIQYTQSQKTSDYSLSSQQFNQRPFRYENQQSVKASWQKLKPNETDNDPITKRDRTVKSITVLQEIFSCTNQKKLIKLVSSFLQSEHQLTQITEQLDKQQIVSLFFKIHQMQSPQLVQFLHLLYDSLFSLDSSVSSKIYTNQDVLKYMILLNYEQIQGQAGQLTRILEILSEMQNLNPDFQVFEEEFQIDMEEISNSFNRVQLRILFLVSSIDILRALKGSVGLFERLEQFVFSQIKNSQIPNISDMQTLIYAYILLSEQLDIANKLGYSKQAVEIIGKSSDYSIYELKRRFMNNALRLSQKYLGSQVYDQISSMNRSFSPGFNKQQINHFNQSENVSYQFLVNQLKMDYTLNNDENFQIDFKCNNLLTNPIIYQLMDKLLPQQIAKKEKVNSIFTEQSRVEKNNQPIIVKKQFSIKQLKQENMVQSQTQNQVSTPTQKNSQQMSQTQGFQSSQFKQQANIIVKPGLDSNEFEILQNKLGRQQQELEELKQLYNNNMVQKKNELNQTNQQNQQNDQLKKKIELLETNLVQLKNENTTYKKEKDQKQSEVIELRSFIQDLQDKQLKLEKLVQQQQQQYQQQQQQFQLQLQQQQQQASPQQLPNLNPAQQYLKKQQSSQSTKTNTQLQMNIQIPTNNLDDKNRLFVRAQSQMSHSPGEEFKNEMDDTSPRIIKQNQAYLLQLLDMLDLNTSFTKTYTKLHSNNENNSWESDVSIVHNFKLEGLITDSNDGKVLMLKAYNQQNILCTESISFDLLKSLLGYVDFQDTLPTNLPNISTTHQFFKFLILPYTAVVQDENTQQYKIQLWPKPYGLLNGLNLKIDFLDLNCLVYVHHLETDQFRIIIFDPSSYDCFKLDLEMDYSSIDTFFVDAKSIKDEFNYYCKSNLLKLTEKTPKFGDDDVAEALVERHFSKDKVKIVQADIKQNQSYRQENLLLKQPIEFLKFIKSIVQVFEQQLKLQQITFPNSLLGMKYFRCKTWNAGTKSQIQIVKEQLDQQQISIYLANCFESFGPNKSKLKAKGTTHINFSAIQREFAVSYDKLQIEERATILQTILYSFNLNIFEKACEQDEQQFDKNPIVQNIYDCGSYRRVIAFDNGKIAQVTIQVIGSNRRMCGIKFSVFNIDETIENGVFLPVSQSEWDTRAMEKQKIKASVQNVPFAEYLLTQILKCETTQIILFKKILNADFKSNQINSNEIKKRKTFIERVDNLLTWQEVIANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QI36</accession>
    <name>A0A8S1QI36_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1640066</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1640067</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000171">
        <property type="protein sequence ID" value="CAD8115469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000171">
        <property type="protein sequence ID" value="CAD8115471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QI36"/>
    <dbReference type="OMA" id="FETYPKK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8115469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8115471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="253" mass="29969" checksum="109535A65806C207" modified="2022-10-12" version="1">MIKNLHRTVIIRTSNSRKQSTSSNDSDSTSTKSEIKFKQPIFIQQPLQPLKEVSEPNTPDTLNFPQTQKVSSRKSRFQKPSIQIKQDSSSSSSDSSSDASEHNVWDDDEILLKGVHLGELSYLSPTDELQMLYKIRFETYPNKKEQISEEQLALETQKLINKLKFHYLKYKKILPLENLKDYTQKYNINDDLNYMRFWDYFRVRTNHRKHKQVIMPTKNTKENRSQQPLNPTIRSQQRIKTEYRIKSLITRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKG1</accession>
    <name>A0A8S1QKG1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1700011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000179">
        <property type="protein sequence ID" value="CAD8116193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKG1"/>
    <dbReference type="OMA" id="YSQIEIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="481" mass="57535" checksum="9D94B0900D8D773B" modified="2022-10-12" version="1">MQRQNHKKLSLSLNQDYILNNYKDSFYYHTMAVRQQQIPKRKVSPIIPRQVKPSRQIPLNLDVYIDQLKTADSQLNSKIKADVNYTMVYDQEDLKLSRKIDQNYTHFEKIRHRSTINPSTIIRNNLRDNFQQNPENFIIQTQRYTNRKQIMPLKVYYQTEIGRITKQFCQNKKKLKILNKFRIITKIIGRLLLLYLRLAQHISVDYQAQKAKIDHYNSLKTAFKMDRKIDDQFIQKIQEWASPAFQKILYYMEKNYEDNINDELIDSDKIKDQEQLWCLNYAKFLFQNIELITRKGNIPIEIIKELSKATQITQNTYMSLFLAKRLKFQISSFSKSEYQLIGSEYIYFNIILSQLFDQVNKLKYKHLKHNLQQKIKIIELASIIHVYFIKYFVNMPSREDVSDKKLYQRKLHISGDKNQELIMIDTKDICLQEIIIVGLKSEEHVKNLLKKKETLSKLLGKLFDKTIQNIYSQIEICDNID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QMY6</accession>
    <name>A0A8S1QMY6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1850013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000194">
        <property type="protein sequence ID" value="CAD8116969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="760" mass="89007" checksum="A41FBAF8E2EF28DE" modified="2022-10-12" version="1" fragment="single">MEFEQFLLRVLSQLQFRRRLIILQFKEAYTIIISTQVIARRNNKGLAIKARSELSSKKKPITKYRYTIQKAFKEISDLQILTRKYLQYKTLIFNVLVQNIQIKLLQLLLANIKSILYCQQIFKCDSDVQLKQRQQNNDHKKNHLIYRIFGFKHDQSQTSFQSSASYSNGQNLSNNSFLEIFCISATYNITLFKVISSQDIGILINHPDPSQYQFSLISIQVFANGNIYILTGNDGLIILQLLLNLELEFIDRIHLSSDDGSIKEMIGVIYNQRVELYENRCYSSLYQLDFISTYSTLIQISQELLIIQNKGQTYLINIFTQDLIHKEVLEGIQGILINKYILSNWKLRFQSGNQIFQQRSFLKIRALDKFNNYCQSTIIFRVFNSSDSQLYALQELELPDVYFDYPQFTSFNTPVSGPDIRFNTNKTQLQLEQKQVEFQMFNHFIMIFHKLNKQILQKSLVQIMMILNLLLQCKSQQLKILLYIFVQQHHPIITQKLNHYVQITKSYLLKRCTIRNYCQNTKTINMYSISQSSTDFQRSFLYDSQSILNIEFISIISNNLYVILGDQQIDIWNIYTKQQYSITYNLIQNLGFSGNWKIRKVIGNSIYHPSLFFIINNDNIIIADFHKEITIIKCLTYQDAVIDISVGYDSFFVIIISTNIQKIIEYDFTNYCNIFQMKELSTYGYQIQIPLAISTSINSGLLYIIASDPIIGESKVILSFKPRSPLRDSLIRIIKPKRDLNQIKDTMISSTGYNNYIIXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNJ4</accession>
    <name>A0A8S1QNJ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1800013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000189">
        <property type="protein sequence ID" value="CAD8116761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNJ4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="82" mass="9369" checksum="13948ECE312EB0B9" modified="2022-10-12" version="1">MEQIKLELALFPLVQRKIIDKTIGRKLILILQWDDKEFIGIDKKGQVGSKKRSIVVVEQSLQSQMIESITGNLIVYTPSKMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNL4</accession>
    <name>A0A8S1QNL4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1800008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000189">
        <property type="protein sequence ID" value="CAD8116754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNL4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="41736" checksum="F6E24FF1C771EAAC" modified="2022-10-12" version="1">MAMIQQFMALQTRFIDKAFINNSRTLMKESIQFLEVNQFGLKPNFLGCIRLLNHIIVKQQDFIYYLGHPSHKIVNLYIQQDQTILLLHLRAHLKQKEYMKEYNIHHHHQLFNGNVMGLIMNLKMHIVDFIIIIQQYIIVSLIVKNVQVKMIVHNGIIATIQIQLNFLKQIAIVINILIKMLQIALYVPLHVQHAHLNFTVQLVKNHLQILNKVVYAKTINISILIIVMIVLQNVNNVQTLNGALNAIKVITVFILMVNAIVKMDIILLILIQFVINVQHIIVMFVIKLMEQNVILVNVVINQFKTIAFLFVVMEQKLQMNNVMMGIQQEEMDVLLVDMIAIIPVNFAIKESVQNVMMATF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QX55</accession>
    <name>A0A8S1QX55_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2800001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000289">
        <property type="protein sequence ID" value="CAD8119030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QX55"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8119030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="843" mass="101655" checksum="2DBF8C928CE8F739" modified="2022-10-12" version="1">MHSELNRQFESSKIERRIVTRVKRRRSIDYSYSQALKSKNIDYMKGHLNRRTRYSNSFSAYDQNEAYYSQDCPNELLKEQKKDNFYSKSASDQSSKQPIQTNKKYDLNDMYAIRKDEVDFRKYNYNFQLDVLQLEALYYWLSIFGFQRSAPLQIPPYLCQYIYSLILYRSDFRKILIQYMDIYSRAYRKFNLIVNFYLNSFIHKLNLDYEVLLDDFLKYFQLSNLQNDKIEQSLKEDYIYRKNNIALGNIRFFFKQKDRILVDFQQPIGKFEDQQFYDYVQFQTEFGKQLSQYESIKSFNQHLTEPHSERTLILFLLADIELNKSIYKTLNQDLINQGKIGKIDNLTKIYLEITTESRLCSRCVIYLQKIQVNFYFNVDQKFQFIIISQYLLDPQIEQEVEKYQKIWIRTPQGQQIQQQQTQKQFQQNFQTDSNYQADIYLENFLENFQAPKQKENLLDSQSVPKEDLQNMNLYQQIEQEEIKEQELLVCYEEQEPTGFVSFLLLKERYCVGNCRNFKEKVDQIKITNTFFLSNGKQEEPKQNSQSSEQEIKQQFANLLKEFSNIKQEKQFQVQNKFQKHQQTFITQEDLLLETRSQFYKLLVKKFTSEFSELNTQQFNLNTILSIYWGIGSLQGFKESHTDLIDALKEELRLNSIIQCGKLNMQLFLSDYTFLREFITPQISRQIRRKNENLKFQLIFVKQKYLVKKTLANDCNSLSLLIEQQVNTVIKKFKNILIIILSVDRIQQQCKERINSQILSFHHSNLELKYFSIKQKEKQQMQQSQQKQSQILEQEWDLLEIEYYKQRYTLQKEGLFLSYKQLSENVDEELFSFECQIHSQNINM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QY41</accession>
    <name>A0A8S1QY41_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T3190001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000328">
        <property type="protein sequence ID" value="CAD8119410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="371"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="420"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8119410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="466" mass="53686" checksum="696BD3BFBEFFCED8" modified="2022-10-12" version="1" fragment="single">MYSPSQVSMLKEEFQDMAIQKLKNFKLDDTQELTKQSMAVKSMLSQIESTMKKIWEELSESIKQIYNLMELQNKSYIDLINENTNLFEISNTELEKLIQILEGKQLGVWNAQKNFYLKQLEKVKNLWGQQIKSFNEKIKIETLEIFQQQFNPSTITTKPIQEEVEVYQIKEDLYEMLSYIQDIDESLYQKILDIIRKEKVSDVIGFLSKTNNHQFFQSLNCKQENIKDVKKQVNKITNVLRNIQYHKFSKDDYSSKTYEKARSDLIKQLKDNKGIIDFIKFIVLLTSIDGKFIQCGSNGLNLIIGMNVDIRNQSLVNIRIKNTSLIGGNLVRCNLNGSEFENVDISGVNLNGAQLFNCKWKNLKINELNKLDGHKECVNSLCFSPDGNTLASSSGNLYNYSNDNSIRLWDVKTGQQKAKLDSQKYAVRSVCFSPDGNTLASGSYDKSIRLWDVKTGQQKAKLDGHX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMY4</accession>
    <name>A0A8S1JMY4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMY4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF197">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="201"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="424" mass="49536" checksum="A2FAC0E99A85B861" modified="2022-10-12" version="1">MDLDFIFFPAPKETLDNVYFDEGRILWIPKHSALYPNFQNYVKRKTNKNMVIFLRQSTQQVEEEQLALIDDQNELQKQPIPQFPNIHHSNKQNLVKNIQVEDKEIGSLEFELTDQEEDLQDKISKLNQESVYNIGKSKINNIQQNSFMRNKKRETTSKSSQHFLQFNQKQETTIQQIKTRQRDGKVIGYIPSLLIKYENSSNIIVYFHGNAEDISQSYAFLIHLRNQEKISVLAVEYPGYGKYNQVQTSAEAIQNDADYIYNYLSKKIGYEQNSIMIFGRSIGSGPATYLASKYKPGCLVLMSPFTSLKDAVRDYVRFVGTWVQHLIRQRFNNLDNINDVTSPTFILHGKKDDLIPYQQAQKLQENCSAKICVLHLAEDMDHISYKLHSDLIIPLMQFLRKINFFQIFFKRPKVPTKLYHNPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMZ7</accession>
    <name>A0A8S1JMZ7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TIP41-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIP41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMZ7"/>
    <dbReference type="OMA" id="CEYRFRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007303">
        <property type="entry name" value="TIP41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF16">
        <property type="entry name" value="TIP41-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04176">
        <property type="entry name" value="TIP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="39694" checksum="8BF134993E4934DB" modified="2022-10-12" version="1">MNINQHKTYNDVNIDEYEQNGWNFIFTKAGMYNTSDLDMLQHALNLQGIPDITNGNNCGLIYNKQFNFGFLVDPKDALYLCNYQARDSNYIDIKKELKSNKLNYISMIPEQAQIKYANIWNQKQNADIKILNKISDTFYSSPYKGTIVDGDQISYQFQRFKQISGIKGKSIFEQQLNENLIKNEVDYLNQVYNTNSKTFHCEYTEDEIPLNNLTPENPIKWAAHISLFEDELGDNGLSNCEYRFRIMGDCFFGLIRNYLRIDDVLVRILDTRIYHQFNWNYILRDFTHREDSWANIQKNGFNFTPQWLTDQNQSFLIQNSVPIQLHINDKIYFKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRC2</accession>
    <name>A0A8S1JRC2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRC2"/>
    <dbReference type="OMA" id="PQIESKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="175" mass="19934" checksum="C59765D126B85451" modified="2022-10-12" version="1">MQLQIPKTKTNQIRPSMFLNNIDMNTVLMSPKLNPSDLEKSPLITQLKSLKKRQATKLPIIKEKQSISISNKESLENTLFNDSVTQIQSMKATIQSGGQLQLPQIQYSSPQNDTKHKKNLTEGNLLKKRVEFRASILVIDFINQIIKKDKIDGSAKPLIRKVLQRQQTKFLPKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSJ7</accession>
    <name>A0A8S1JSJ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSJ7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035841719" evidence="1">
        <location>
            <begin position="21"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="95" mass="11574" checksum="529AA9660055A863" modified="2022-10-12" version="1">MSKSLLIIFVLLISLKSIAAEEIDLSLIRVPNYITQEDDIQLYKIRKQQLEFQKSKYMKMSKEERKKVEQQSLAFQKWENDLKRKYQYKKRQDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JT80</accession>
    <name>A0A8S1JT80_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JT80"/>
    <dbReference type="OMA" id="CNFFWIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="491" mass="58233" checksum="E07CA59A78C7EA24" modified="2022-10-12" version="1">MVFAYLKKRCIEYQYNPNLKKHIAINLILLVLTIIIRALFEIYLRQQLTSLNEFNQVQSEQQSQENNEIQINNPKLQIQNNHLKLSYMILDQFLHLMLSLEIIALIFQITLIINKEKQELPILDPLLKEQKDMSCSLQFVKSSNYGISSIQNNQPTYNSIEMLTFSRNKGTPNQIKNQFEERSPLNVKQYQLEKNVNLQKQEIPFIQDQNNSHSKIQIQSDNQILEIPSNKSIQKIPSNKKINQVEQSNTINQTQQIIYSKILGRAQKCINGKYQNIYIIQKCEKQENCNFFWISQIKSTNYFCLNCDQKNIIVQNNEQVEIKNNQNIKINQFFQSNKFEQQLDEMIQIYKDTQLTFCHSFQFCGFFWIKNKQDNQNEYYCPICQETYSTQNGENKDTIYKDIISQCSYCCVNTSQYFQATCFHTYHFECLKELGKNQLQTKILCIICNMNMMSCLKKNKMLPQETFEKIFTNQIQRIREETMHEGVEDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTB3</accession>
    <name>A0A8S1JTB3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0050077</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTB3"/>
    <dbReference type="OMA" id="CVSETHM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056158">
        <property type="entry name" value="Beta-prop_IFT121_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056159">
        <property type="entry name" value="Beta-prop_IFT121_TULP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057979">
        <property type="entry name" value="TPR_IFT121"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056157">
        <property type="entry name" value="TPR_IFT80_172_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057361">
        <property type="entry name" value="TPR_WDR35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017233">
        <property type="entry name" value="WDR35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056170">
        <property type="entry name" value="Znf_IFT121-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722:SF8">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 4-LIKE WD40 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722">
        <property type="entry name" value="IFT140/172-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23390">
        <property type="entry name" value="Beta-prop_WDR35_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24797">
        <property type="entry name" value="Beta-prop_WDR35_TULP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25768">
        <property type="entry name" value="TPR_IFT121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23387">
        <property type="entry name" value="TPR_IFT80_172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25170">
        <property type="entry name" value="TPR_WDR35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23145">
        <property type="entry name" value="Zf_2nd_IFT121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037536">
        <property type="entry name" value="WD_repeat_p35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="IFT121/TULP4 N-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="IFT121 second beta-propeller" evidence="11">
        <location>
            <begin position="345"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="IFT80/172/WDR35 TPR" evidence="10">
        <location>
            <begin position="689"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="IFT121-like TPR repeats" evidence="13">
        <location>
            <begin position="1008"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="domain" description="IFT121-like zinc finger" evidence="9">
        <location>
            <begin position="1140"/>
            <end position="1183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8043426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1196" mass="137537" checksum="4EA05A1210B81238" modified="2022-10-12" version="1">MFSFLVKKIGMLNQGKVHCIAWNQEEGWIAIGGEHAPQGQKKGLLKIIKLDDQKAGIQGQQAKLPYDTILEQHTGKVNIIIWNERYQKLTTSDDQGQIIVWINLGNEWVEEMVNNRQKSNVTDMKWSPDGFKIGIIYEDGAVIIGSVEGNRLWGKDYQYRLGLIEWSPDSKLMILGTADGQVIIHDQNGNQLNSLKMTCLMGLVDPKNYFAPNQQIAAVQWYEYGKMYTDETLPGLLIGYTCGRVQLMKNDKDESPILINTDMIVSSIRWSPNGSMFAVAGSLKEKDDQRAVVQFYSNMGDHLKTLRVANTDKVNSLSFEGDGLRLVMGVGQTVYVANLKLDYKWSYLSQSETLIFAYQRQDRMEFTIIFWDTKREQKSIRYMKGLLDIKGSNDCCVLISLVEQDTWKVELCNSIGSPLDTKFISIDPKYSCMTKTHIIIANNDYVYLWQYRNQVQRLTTFESNQNTGIRKIGKEMAWFIDENPDSQVIYDKDTFNLEKQTDEIICAIQANENYLFIGRINGNILKFTLPYVSVEPKYFLENRPNIININCNSTRLSIIDIMGNLQLLDISVASGKLLDFDKKDCWQVVWSDNDPLQFAVMEKGRIHLVRDVNAEDPYPCEAYLCSFSELTVRGALLDDIMMSPDGQLRADELLVYYESRLFKEIKESIQKTLPKEMFSIIEKQPSNTLWQLLAQKALEELDFSIAEKCYLKLEDYAGLQFIKRILDYDEKEKQKAEVYVFLKRFDEAEKILREIERKDLAVQMRMKTGDYQRVLFLSKDLVGADEVTVQTQNKLGNYYCDQGEWEKAQQQFKLSNNIEKLIETSFMIEDYQTLEELANSMQEGNALLCDLGERFQLLGMAKSAIKCFEKAGDIKKAIDCAVLLNAWNFAVELAERHNFVQIEGLLQQYAAQLLEQRMKLEAAELYRKANHNSDAARLLSQIGEDLIKSDQSPLVVKKLFVMAALEVDLYKKKLYDVTMTAQNNTIAHTLDQLVTNDLNHSADKVLNNPWRQAEAWHFYILTQKFLYSGNYKYALKSAIRLCEFELDMDPKRIYSLLALAAYYNKSYKECARAFVKLENLENITEEERERYQQVAAQIFVKNAPEDQVCDAIQCPKPSCGNKKITEYQLFCKECGTIFGVCVASGKSIYGQKFYTCKACKHKMIDQEVQSQALKFCSLCHTPIDFNRFGKHGDKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTC6</accession>
    <name>A0A8S1JTC6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTC6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8548" checksum="55C51854F225863D" modified="2022-10-12" version="1">MQVCNKLPIVFIQECLQLVSVILIKSICFITLLAIVQLVQIIHFIKNTSNIQQYNNSNFKIRNSNINIQLYAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTM8</accession>
    <name>A0A8S1JTM8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0080528</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTM8"/>
    <dbReference type="OMA" id="EYIIAIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000401">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF106">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="123"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="4">
        <location>
            <begin position="290"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="539"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="578"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8044959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="634" mass="73431" checksum="00ECD28510C5A3E8" modified="2022-10-12" version="1">MNQNECFEINEKLEMFISCRQLDDLDTVSDYNPYVIIYKKNNNYWTQIGQTELIKNTMNPNFTTSILLEYHFEVQQHLKLEVHHFINSTQSKIIGIAETTVAIIAGSKDQILIGDLVNISGRRSGNFIIQANIVKQIKEELNLTLSSYGIPETRFWFWHGTSPFLRFYRLVQDDNIPVLVYETEFVKDTKEPTWKEINFKSLHLCDGDYKMQIKVELWDHRKNGKHLYLGETSFCVDELIEYNIQNKILKKEFKNKMKKQESSGILQFNRFLYNPNYTFIDYCSGGQQLKFIIAIDYTKSNGKQNDPNSLHYIPKNGGSNQYLQAITSLVEILINYDNTKKISLYGFGCKPKMNLINTNQTLHLFPLNDNPDDPEVYGLDEIVQCYKKSLPYLLFDDPAQLTPNLKNAMNIANQYKQNQGNQDYLILMILTDGQVSDMQVFIDDIIASSNLPLSIIIIGIGNGNFDNMSVLNNQFNQILDSKGNILVRDLVQFVPFNQVKNDPALLAKQVLAKLHNQIIQYMKLQNISPKPTDRKKLLLQQQNPQSSKQQQLSQPQKSQQQQLPPQLLYPPQLIYPPQLQYPNQPSPIQQNSQNQYQQYHPQTGEINNNNQGQGLQQQEFLKELVQQTVKYPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JUN8</accession>
    <name>A0A8S1JUN8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100424</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0100429</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JUN8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8046534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="91" mass="10257" checksum="892BDE3D5010FF75" modified="2022-10-12" version="1">MLNWLILDSQKLDSIIEAIESITIPEFADHNAPKFNIIFDLKLTQAQQVIKLSTQNESSKNKRIVGTPDQIAPEVLKGESLTNSSLDYWSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JW05</accession>
    <name>A0A8S1JW05_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AN1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0080181</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JW05"/>
    <dbReference type="OMA" id="EPKPCAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050652">
        <property type="entry name" value="AN1_A20_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634:SF67">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01754">
        <property type="entry name" value="zf-A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="6">
        <location>
            <begin position="79"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8044616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="144" mass="16762" checksum="9B1E49B77DB67FDD" modified="2022-10-12" version="1">MQNNQQAATEEQSKNQEVKQETKQCPLCQTFFGSAKTNFYCSQCYKTITQNTNEQQPQQQQQQQIPEDKAQSNQQVNIQQDPSKCYVCKRKLGISGIQCKCKIVFCNKHRLPEDHNCTYDHAEKARQLLIKNNPLVDHKKLEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZN6</accession>
    <name>A0A8S1JZN6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050302</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZN6"/>
    <dbReference type="OMA" id="TLWSKNF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="335" mass="39593" checksum="6B94C3AEB4938CAA" modified="2022-10-12" version="1">MSTYSLYPVMHEANAFEIFSMLLQIGLDRANNTKTIQQRLNQTEHIKTATDYLSFVNVNEKCLSQSLKQEKQRVEQEFALIEMLVPNKSKTHLMSFYHLSLKLITQWLPQFQVKVNLSGEFLDIAELYGQFFIASGMNHRLQQIQAIKKNNLYDKIPFMPLLMKWLHEYCRSQFNKLFASLYETITQMKSNYRQIVTKPKEEILSPRVNKMPINRPTSCIINKTRSISNYSIRKTSQVITKRNNNQAVPKLINLISDVIDIDLTHYDQQTHKKKEQIYSTLWSKNFQVRCTHEKYQQTYGIQKSQISSHFFNTQNKRPNSFFKHSFHIDNNLSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZW0</accession>
    <name>A0A8S1JZW0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0130023</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZW0"/>
    <dbReference type="OMA" id="YQIIFNN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="9">
        <location>
            <begin position="35"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8048658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="203" mass="23892" checksum="20DFC18DBFC57936" modified="2022-10-12" version="1">MKYLFIYATFLVSVYCFYDFIPDDIITFTLTANQEDVLLENITEPTLIKGAYQVNHHKDVIDFSVKTPTGRTIYSKMATNKGNFTVQANEIGLYQIIFNNKKKANQLLTYAVDVVKEKEDKIKSTDIDPLELDIDYIHIGLQELYYDHKFQQIRYESSTQQVKEANKKIYAFTVIETIFIVLVTIWQIWYIKRLYNKRSPLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4L0</accession>
    <name>A0A8S1K4L0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EFHB C-terminal EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0130402</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4L0"/>
    <dbReference type="OMA" id="EANYASH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057428">
        <property type="entry name" value="EFHB_EF-hand_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040193">
        <property type="entry name" value="EFHC1/EFHC2/EFHB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086">
        <property type="entry name" value="EF-HAND DOMAIN C-TERMINAL CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086:SF12">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING FAMILY MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25325">
        <property type="entry name" value="EF-hand_EFHB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EFHB C-terminal EF-hand" evidence="5">
        <location>
            <begin position="340"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8049313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="416" mass="47695" checksum="5B5E612059894A86" modified="2022-10-12" version="1">MSGKTRDYFGTLKSAGRTVLKEESASDCIQPVQNQVPETPPHIKKYRKSYKHQHGCTILHPGLVDAPKPQGNWLYGKKTDPSDKAGDLFKQQPEGIKELINEINEQKYASHIKEPLGTMPTRNYNWPEESKSDGFAFGQKIPPSEFTAKEVVFPPDAKRDEESVRLMYLKSHGNFEAGEQKNREYKWNVNPNEYRFGKKDEREQEQMKKILQHELTQNQYPKTTIISKHQEDWKNYNEDPLGKPKNLAQINSRMPAIFGETKKDEQWTAGQCINGQPTQKEVQPDIDLGKATKFGFRNQTKQGDETRAFGVPTIRNDIVKTGMKSVADPQNYGDEVPAVALLFPEKFSHMGLTEQDFLMLRNKKDIKQIFESIGIKYGIGKFEGVFKRAKEIQNTFDDKVSVKAFQLAVQEMHHID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4L2</accession>
    <name>A0A8S1K4L2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0110322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4L2"/>
    <dbReference type="OMA" id="KFKHINQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="168" mass="20115" checksum="CB2836BBA7A7E03A" modified="2022-10-12" version="1">MKQEDVKNLELSDIEINYQINYSLNKNKDSLIQHSSNGVITELRSFKDYQTIIKIKPIIQNNKMNYYIHFDIMSEISNYINNKDINLRMCTISFEQQQQFQQNQMQFQLIYAITFEYGFQIKENPIDQAIIQISSRIDKICIETYNNGDINLIQQIDELFEQFQEFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6W5</accession>
    <name>A0A8S1K6W5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L37a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6W5"/>
    <dbReference type="OMA" id="DAWTHNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15483" checksum="660EC7F50D65FF9D" modified="2022-10-12" version="1">MTKKTKKVGITGKYGTRYGASLRKIIKKFEISQHQRYFNTFTGAHSLKRQAIGIWRCTQTGLQIAGGAWEVNTPAGLSAKQGMLRIKKLKEDAEVEVKDEKKEQKKQQPKEQQKEQPKEKETKKPQAKKQQAKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K837</accession>
    <name>A0A8S1K837_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0130330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="SHLNCHT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052828">
        <property type="entry name" value="NELF-A_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13328:SF4">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13328">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR A NELF-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035908528" evidence="2">
        <location>
            <begin position="23"/>
            <end position="3298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8049216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3298" mass="378877" checksum="C38D283FB12941BF" modified="2022-10-12" version="1">MKKTLITFLLLMVMVISTKIEASELRTQGMTYTEAQNLDVSHLNCHTDFEYIIGELARWVDIIDNREQLIKDLAIINQVISIVEKAKGTISVIHQTMLVQIQNKVSKSLQSIFTQNKWAACKVEDALEYVAQLNSTQTNEEKIALANKIIDHLRQTQEQIHQYLNQCQYAKRTQGLRQKISQIQTLKRVCDQEIKKPTIIIDNDPDDEVINFDEEPEEIVYYYFEEEDIETPPDYIYHEPQHYTYPTYQDETKKPQKKQVKKIVPKEIVEEIKGNKKYVALGHEIEGEGKESVDEQQNQQEEAELVVQAAKVSKIEGLNNDEDDSEKTDTDKVKGEGESKEQKNGEETQENKETEQQQVIVETHDKPLKQVESETKENVTVDESKNGNADEDTTEVKVNTNNKTEETVDETIETKDTNKKESTEESSQESSETVTTQKSVSSTQEKQNTQSETKVEDASDENLDQKSTQDGNNQTTDKDEDIVVDNESKSQDDKKDVQIEQSKVEKVDEHKNDDQKQEGEVKQEGEQKEQGEVKQEGEVKEESEVKQEIEVKQEGEVKEESEQKEEGEQKQEGEVKQDGEQKQEGEVKVEGEVEGEVKQEGEQKEEGEVKQEGEVKQEGEQKQEGEVKQEGEVKEEGEQKQEGEVKQEGEVKQEGEQKQDEDHKKEAPKNQDDEKNQKKQKSSDDRSLKIQSLREDPNPNEPTQFQPPKHRANSGRVQERKVVAVNTKYQHSDFDGEPTEQFEFIDRALLQDSSEYGYGYWVRYAEHGVKEHIREDGEYYFLSRMTINEEYEDFSFYGDRALAVFMFDNSFVFSTYDTSEKLKTKDKAVVLNENMDSMWYFVMFSYSNPLRKAVGYIVSYGEGNGIYRVEIEATHIPPSYIKIIFGGKHLGYNGLNGQFANIFFDIDAPAFVDGDEALDEVLKTLSNPPQSVPAMIDETVIQKPKHLNGNDKGEQYHFDPQESQLIIEEYAVGLWFRWIDDLKVDEPNTFQIINLRSNKVKTAGKGVLGDRALEIHHTYGGGAKSTIYFNTYTIKGNRAKGQPYLSKTVESVEFIWTYVYFGYDNDGGRAYGAVIKPGLVGEIKYEGIQHKLVNSLSVTLGGDNVISPFNGMIGYVGIYLGTGAYREGLGFEMTFNYGEGVMGVYQVGKPVTYIAGDANQAKYVAYDAAENVVDKIIIHNEEGMRINGQSEYGFGLWTRWLSTLPKYLNKRAPIHTIARMGTQGYVIESIDGKLVRSNANRPNTPKDNTLSISLTPDSYEFQTLELKDDIEFSQLEGHWNYVYFGYIRHGDQGLAKGYVQFGVDGEVDEVIFDTFHDYLLEYVEFIVGKTSAPLFNGEMARISFSFGPGSFIHTIETLKIFTQNTLPEKAQIHPVARQTLQLVGAPQQIKEEPIQFEFDKYQGAEEYAISGWVKWNGPLVTGKVSHLITMAQKRLEDLDGKNEETLQIIRGDQAFTFVTYSQNLGEYKLVTQDEAYGEYADQWTYIYYGFSQQKQQTYGYLKYTFTESEFRQEKVNHFYLAVFSVLIQEKQQYTQFIGQIKTWVINIGDGAFREGGFDENENIKVHFGFISGTDHIKLQQAGQEAHHEETILECSSQSDEVPLHIEFQQSDKLHLHGVSEYGYGYWVKFQYFANKNTIYSRPQLMGLSRLTSNQDYKDFDNPGDRVLLVLLGKQSYHFGTYDVITKSNNVAGDIPYQIDSESEWTYIYFSFKRISQTQGHALDIFDMGYVQFGVDGEVDEVIFDTFHDYLLEYVEFIVGKTSAPLFNGEMARISFSFGPGSFIHTSETLKIFTQNTLPEKAQIHPVARQTLQLVGAPQQLKEEPIQFEFDKYQGAEEYSISGWVRWSGPLVTGKVAHLITMAQKRLDDLDGKNEETLQIIRGDQAFTFVTYNQNLGEYKLVTQDEAYGEYADQWTYIYYGFSQQKQQTYGYLKYTFTESEFRQEKVNHFYLAVFSVLIQEKQQYTQFIGQIKTWVINIGDGAFREGGFDENENIKVHFGFISGTDHIKLQQAGQEAHHEETILECSSQSSEVPLHIEFQQSDKLHLHGVSEYGYGYWVKFQYFANKNTIYSRPQLMGLSRLTSNQDYKDFDNPGDRVLLVLLGKQSYHFGTYDVITKSNNVAGDIPYQIDSESEWTYIYFSFKRISQTQGHAMAFTHYKDTTSGIQMDVMHSLLNNYLQLTVGNAGKYYSNFNGQITTIRFNLGPGAYIDNKQGLLQRIKNKDVMPDILAPSKKYEVLIGKHDVTKIEEDQHITHINEEAREYSIQLWFRWFKLPVKTQQLIYRFTSSNPDSLGDAQKIGDRTLALFHTDGIEFSTYNLNPLSLQNTYEAMIPQQQLEVWTFVYFGYSKHHQKISYYLLADDDEHKGLEQALHAVSTNYWLFLVKDAMTKPFDSRLAQVILNIGDGSYREDNFNTLQVYLAAPKLFSSDSKFDWEADDTITLVSGDPEKQGMKITFSEPDRKIESVQEYSVGLWTRFLQAWPERQWHTPSEMQILRLTYNDEVDHGKIAIGDRVLNALVVLDNYQFGTYDLNDDAPNEISTIPYNHLEGRWHYIYLGYKRQLQQANYFVFDGEEIKHATNEKLLHKPLGDYIHLILGGEKDVAPFQGLFTEVAAHFGVGSFILSGEELMKSIDTSFALPQELTVDYIHKQKHGQQQLIGESDNNEGSESTGDTWSGVGEYAISGWFKIAETQVKKEGEINSPCQILFRITNNDKEHLSDRKSQGDRALHAQICTSDTVKLSTYTMKGLKDWNEAKFLEEKVELGNSKKAWAYLYMAYNENAGEVQTLLHLFEEDKPVIFKGVQHFVPHFIGIYVGKDPHSRRFQGELQKWVAQYGQGAFVDVMKKGYEDTLPNFRHIQINQKYLWFEKEDRIIQAPEKVEQTFTSETESVDEYAVGVWTRWLIAFPTTLTDRADVHTIFRFSSTRQYQDKSELGNRVLSAFLTKGNYEFSTYDASKPTNAVDAQLPYENIEGEWTYVYAAYKNKNFYGMVLFKDQQKAAHLNIEVTHQVLTGYAHFVLGANEFGYKAFHGWFYDPRIFLGAGSYISESQKVVEMIHKLHRKLPITPQQAEDFKWPVSLLDTTNQDDLDSKKDKLNFEFTDKAEMQSYSIGFWFQNAMLLPEMENDFTALVRLTTNGPDLAADDKFIGDRTLALFTKTDKLMASTYTIKDPSFEPLSHTFDIKEHQWTFVYFGYENHHARAYVLTPKGPTEKIFIAQHIIPNTFYLRVVNDLGHPSFWGKIYGLKANFGEGSYLENPLELIDKWPYDPKQHSDIDKQGEKVLSINSAKVTKQKNDDAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8H8</accession>
    <name>A0A8S1K8H8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0110097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8H8"/>
    <dbReference type="OMA" id="DIYIAWP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="372" mass="43531" checksum="374D39AF113EC9DC" modified="2022-10-12" version="1">MLTINDFLKNQIQSLTECYLEQSHKSIILDFSTFNDETQLNYLKSLLHQPLIGQENEYYEFHKERLENLDNFEQQFLEVFYNDLVMKDFVSAMILAKEQFSRKQILDEKNTPLEFFQTTQSTIKRCCLLKDEDPKELATELKNFGYQNKPSIQFVCLKVEYELGKQQNENLEVICKKVKDSQLSKLVPILDVQFKSDNIQNLYHDAKFAYSTINAALLNQEVETEFMILCTNIVDLAIQYQGDIELNGDNQIRNYSDRLIYFVLKILFNSIPPSYGAIFFTFDRGFTQCKKIFHFINNLNRFDIYIAWPFGVKLNAKVFDEVAKSYSIVQRQQSATITFNQILKIFQNCLGGQYKDEAEKEVLQPIKLGSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8I4</accession>
    <name>A0A8S1K8I4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidylinositol-4-phosphate 5-kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170376</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8051071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8I4"/>
    <dbReference type="OMA" id="YSTEEYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="47516" checksum="782489C849B54E9D" modified="2022-10-12" version="1">MSEDNEQTNIVQILEGKQAKNITGYSQELTQQKRKILEDFKGLNVYDDKGLLRSDCRIKKYVDGSRYEGQLVGEKRIGRGINYYNNGDIYVGDWDGDMFNGYGHYFFVNGERYSGNFKNGCKEGQGKYYYLNGNTYDGNWVNNQKQGLGKYYYYSTEEYYDGQWNKGLKHGQGEAKYAYGEIYKGDFQNDKRNGFGIMRFNDGARVEGQWVDDQLNGIGKIFYSNGDTFDGTWLKNQKSGKGVYTMCEGKQIYKGQFENDMMNGLGYLQYESGDIYQGYFKDGKKDGEGEFYYQASKDRYKGQFKKDFRTGYGVMHYANGDIYQGEWFEDQKQGKGKYYYSMTNDTFDGDWIRDKKHGKGIYTFGNGDIYEGEWSQDKWHGKGKYTSKDRTYMYGEFRNHKFVTPMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8W2</accession>
    <name>A0A8S1K8W2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8W2"/>
    <dbReference type="OMA" id="RFFEIVP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="464" mass="55057" checksum="9E470F27DCA82AD0" modified="2022-10-12" version="1">MKQARITRLHPIISPPKNEEAPFFDPCPKFQTLYHPKCSLDRIISYLFLKQNDINGKTFSQRRICKLDATQNYDGPSKCRVLKIHPPDANKINGWQNVGGEVSYDYDNGWKTRLSDICTDIQEDINIMRKVLMDEGEQKHNYKARIEYLRRYIEEINKMYHIVNLKEDIKKLRGIYRKSREEIKSYVKFKGTRFFEIVPQLKSTSKTPTVLDEFKVFKPLSDEKFMEKQKYLDDLFKHLNKKNQQKEVKEQLEQLDQLDKMLQQSGVKIDPIQSDPFVSKDVTVNKEIEKKFVFEEFIEESKQYEDEQEEQKDEINEETENTKGNNQRPKRAFSQIISSKQQDKKSAAQEFNSQHYLFEVKDVNSFGQQIRLNRLNKIMNEMFKEQRNFSQDLQQYSYNHIKPQTVSNISCENLQSLKHKLVQSREKELKRMNKSSAQEIGKRSSVHVSAFYSTATSKQQLKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9Y3</accession>
    <name>A0A8S1K9Y3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0180106</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9Y3"/>
    <dbReference type="OMA" id="WANSKRC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01862">
        <property type="entry name" value="Rab7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000086">
        <property type="entry name" value="Ras-related small GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF20">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8051457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="206" mass="23261" checksum="950C813CADF401B4" modified="2022-10-12" version="1">MASKKKQLFKIIILGDSGVGKTSLMNQYVNARFTQQYRATVGADFMAKEVMIDDRMVTLQIWDTAGQERFQSLGGAFYRGADCCVLVYDITNPKSFDSLDSWRDEFLMQGQPKDPEHFPFVVLGNKLDKATERKVQESKSQQWCKSHGNIQFFEVSAKDATNIEQAFQDIAKAAASQEKDEEIFFPTTVTLTKQDPKKQAKQGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDP4</accession>
    <name>A0A8S1KDP4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0200006</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KTSCHEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF93">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="3312"/>
            <end position="3411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1032"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1745"/>
            <end position="1765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1782"/>
            <end position="1803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2433"/>
            <end position="2478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3435"/>
            <end position="3458"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1038"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1054"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1064"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1754"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1787"/>
            <end position="1796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="2447"/>
            <end position="2456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2457"/>
            <end position="2478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3440"/>
            <end position="3454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8052797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="4124" mass="484406" checksum="3D4FF88E8B88038C" modified="2022-10-12" version="1">[encoded data, 4124 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEK5</accession>
    <name>A0A8S1KEK5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cilia- and flagella-associated protein 53</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0190320</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CFAP53 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEK5"/>
    <dbReference type="OMA" id="ANMQYTK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043596">
        <property type="entry name" value="CFAP53/TCHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043597">
        <property type="entry name" value="TPH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31183:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31183">
        <property type="entry name" value="TRICHOPLEIN KERATIN FILAMENT-BINDING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13868">
        <property type="entry name" value="TPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="2 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichohyalin-plectin-homology" evidence="9">
        <location>
            <begin position="135"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="422"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="53"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="182"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="432"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8052711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="484" mass="59890" checksum="58CC21198783F349" modified="2022-10-12" version="1">MIQSSTRNRSEQLIYQRRQQEQQVGKLNTKLRQEHYEKTFAAWENKGKDVANMQYTKNRLQQIRTEAEAHKNQRKEKLAALLNAEHQQYQQEIKAMVETPEQVKERMMKEVAELKQRKEFERAKQAEAAYERRFRDNADELRLVNQQFNEQQTVAYRNMQMMEKQKMLEDQYEEEMIYAELYRREILKKERLEKEKEIQQKAKVDERNKVLGIQATMNVNKFQRIKDEIEQEKQMLREEWKREEERHKQREIQYLNYKKEINSEIALNNEQQKEYKKQMKQEEKKQDKEMIQQVLEREEAIARMEQAEKLRQKEETRQFLLNFKNRTNEYSLNDQLKERLINEENTRQWETKEAKWKAEDDARVKLMYEVYAQRAENVELKRKLIEDEKNIKQQDKVELLRQLDLYQKELEEKQRLEQEKIMQTKHNLLNQMDEKKQRQQQLRDKKQQEEEDLRRKKEEYDKKIEMEKAKGRVLLDELRKQRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKF5</accession>
    <name>A0A8S1KKF5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKF5"/>
    <dbReference type="OMA" id="DSIFCYA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="479" mass="56729" checksum="B7A66E4493770487" modified="2022-10-12" version="1">MRDLIQINPALQTIIQSILILILKQVIFLIITIDSNPLIILNKLYLHYLGVITTTEINVNNFGGVNPLPSVIADKKNQKFSDSIFCYAFKEEFQAQNTESIQEVTFADFLAPLGQIVHSQFDENKPLLYGYIINSPKILYSYPCYSYGESFEEYLPESRPWFKLAQNASEKVAENLEYEYSVSEPYLFNQAKQIGITLSLPLVDRKLKFKGAWCMDIFPEYLIVITEKMTSQFQSMSIMIVSTQGMIIKDDDYKNAFPVYFYNESITGFDINQWQKLKESDLQQYQFLNSVKGTQQRIIKFYLKKQQLFILFIIDSNEYMNYLMEYQINQQQKINDFLQITLILLLMTFFFLILLAYLIITFLIMKPIEDIIWFFQVRKSKTLKEKYQDLLKTRFRYRNHVYISPTIKVLQEAAYRLNYWNFKIKKQKNAQCNLLQMFLFPKNIHRQKICLNGSKLLDLHQNLEQQEFLGMIRLGYDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTC6</accession>
    <name>A0A8S1KTC6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0270068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTC6"/>
    <dbReference type="OMA" id="QWQNDQK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="353"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="394"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="399"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8058147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="645" mass="76037" checksum="024E4B9BAB7C81E2" modified="2022-10-12" version="1">MNYQAVLDQIKFYEPHFDDYNFLDLQKLNAKLTISNNYVYYGQFENNLRHGKGILLQQNGRKYEGQWQNDQKYGYGWELLANGSQYEGNYVMGKPHGQGKFIWPNGEVYEGQWNQGAKEGQGTWYGLKGEQYQGEWINNVAFGYGEHIHSNGDRYVGNFKDWLKNGEGQEYFSNGDRYQGNYLNGKPHGYGEYFWSNGAIFQGYFKDGLRCGKGIWKRREDSPSDQYQGEYDEDKKNGYGVYKWSNGNEYRGIFLNDYKHGYGEMHYVDGKILKGNWEQGKLVNEIKQNSSKKTSHSFDNHIKIVDNTHSYNKSYQNRNIFMDNQQENNNQIKSNPYNDNDNEKLSNSYADALNSNKNINNNNNNNNNNSNNNSNPASSQTYRTTKTIRIRQYQKDKSFSMSQQTSQYVPSSPKRDISEKNEKQKTQTKFCIKAQDNFYLHIRRPKQIFKSFLIRQRLIYLSESKNVSGKNQLGLILFGIVQSKREGALFNNVQCDQVYTNDYKRYIEFYAHIELKTTYTPLLREIHKNDVIQKSQFQVSIQSKQKKIFFLNTEIQEIVETFFNQFFREQIDQNYKNNFYNSSVKQFSSYSKGSQMNKVLILSHAEFIAELSDIFIKEKIFIFLKMNIHLYKLQIELYEIGYLYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTW2</accession>
    <name>A0A8S1KTW2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">40S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0280130</name>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0450182</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTW2"/>
    <dbReference type="OMA" id="YYGRLTM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.60.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_eS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_eS17_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8058950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8070347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="16083" checksum="EDF1DD0AE94D4FF3" modified="2022-10-12" version="1">MGRVRNKTVKKAAKVLIEKYYMKLTSDFHFNKKILSEVGQVPSKKLRNKIAGFATHLLKRIQTGSVKGISLKIQEEERERRLDYVPEKSSIDIDNLRGDQDVKRMLEKAGLEIDIPIDEPVVEEKQQKQPRRGQRRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXK4</accession>
    <name>A0A8S1KXK4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230309</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXK4"/>
    <dbReference type="OMA" id="YLLINYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12782">
        <property type="entry name" value="MICROSOMAL PROSTAGLANDIN E SYNTHASE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12782:SF5">
        <property type="entry name" value="PROSTAGLANDIN E SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="37492" checksum="8513347F8E49B0A9" modified="2022-10-12" version="1">MIKLRQLFRFSKIQNKSSIDGIIEQRRSINRQLSKEELQSRDLQIFWQSPQTDIVIHLVLYMRHDCHICQKAFSLLNYYNISFDKVDNILFLDFAKKAKIITLEQWQWPVLKVNVSLDSTQFITGSENIVSFLINKELILNQTHMTNTLFAQQGIECILQKIKKPLDLIFLNPILTWQNLFKSSPDSYAKDSKNYLLINYMLKVGYRAFTQSSRNIYHMIRQKEAYFKAQAELLQGLNEWIQRLNGQQFHGGQKPDEADFELFGVIMSRYNSNSFKKYIENKAPFKFYQWVLRMQQLCRYDQNRFYANTDTNVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0R3</accession>
    <name>A0A8S1L0R3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L0R3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="91" mass="11131" checksum="3A2D615AA79D453C" modified="2022-10-12" version="1">MKKEQINNYKRLLEAMPNYIMFDGEGNYGRKQLQDHKNLKVQINLQWKEEDNIEEQNKNYETENKQILPKILNLKVIFMCYIIRNPRMLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3K8</accession>
    <name>A0A8S1L3K8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0320307</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8062057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L3K8"/>
    <dbReference type="OMA" id="EWFYLDT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000100">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="413"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="493"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8062057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="592" mass="67783" checksum="9358CE7C0DDACB82" modified="2022-10-12" version="1">MSQQPLIEIPTLNHNDSQQQVPKEKSSKLQKLSSFDHLSVHSFKSGLTYIRDSWKSGMAQTILNMPFHLTIGSSSGCNASVGVTTAFIAALTNGFFSGSNHSIYMPSWVVIGLNYQLVKTYGVEVMPWITIIVGIYIYIAGLLKWHHLSDFIPVYVIEGFLLGIASLFFFSYSDYMFGLTDNHSNRGVELYSSYYDMFKSFLERGDLNYFIGAWCVFLFLQMGRIFFRQFPWILITTLTGLTLGILYPTEKCLKSAYGEVTIYFNFIEYDGPKFHPNFQVVSHLLIEAIPITLFILIQNQFCARAGQSLSGVKCDYDQEIQCVSTANILSGIFGGLPCCASSRMYILDIKLRQTNQWSSILNALSILFFYGVFSKFFMDIPFYIISGQLLYMIINIPPWHYYVNLYKTQRKLILFYIFCIAWLCVNYGAIQSTLIGSMHALIIFAKNMSSPSAEVMTNQKEGVYQINLRDTNLHEFEEECNDIPPNLDGTFTIYRFNGALNYINIKGHIDQIKELAKTDNFILSFRYVCIFDFQAVDSLAIIIDNMRKNSTQVYVTGLHPGMLEQLSENHIFKEYFTNESNMFHHIDKLPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4Z5</accession>
    <name>A0A8S1L4Z5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0300238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4Z5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="208" mass="25469" checksum="26F2719CFC7B9900" modified="2022-10-12" version="1">MDLVNQAFKQLYIQDECYENIKDKVDQFPYDEVYSLMEFYVQNYLDRVQNNLYSYSYNDHDSAKFYMEQVNSHWMRIQNIALDQETIVLRYQNTIDQNFKIEYFKIIRQIQHQEIYVESINLINQLNLTFVLQYFNTLNQKLQTHQDLMTNWLTYSQCEQGIDEIEDFEKRLECAMTNLPDYEKQQPKLPSNWEKNIINLMKKKKKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L601</accession>
    <name>A0A8S1L601_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dolichyl-diphosphooligosaccharide--protein glycotransferase</fullName>
            <ecNumber evidence="6">2.4.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPRIM_AZ9-3.1.T0330013</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a di-trans,poly-cis-dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] = N(4)-(oligosaccharide-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein] + a di-trans,poly-cis-dolichyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22980"/>
            <dbReference type="Rhea" id="RHEA-COMP:12804"/>
            <dbReference type="Rhea" id="RHEA-COMP:12805"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="Rhea" id="RHEA-COMP:19509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:50347"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57570"/>
            <dbReference type="ChEBI" id="CHEBI:132529"/>
            <dbReference type="EC" id="2.4.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.18" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L601"/>
    <dbReference type="OMA" id="TWYAIGT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004579">
        <property type="term" value="F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048999">
        <property type="entry name" value="STT3-PglB_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21436">
        <property type="entry name" value="STT3-PglB_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="180"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="395"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="459"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="17">
        <location>
            <begin position="28"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="STT3/PglB/AglB core" evidence="18">
        <location>
            <begin position="524"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF21436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8062191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="743" mass="85069" checksum="C1A49CCF87BB31D0" modified="2022-10-12" version="1">MSSEEETQQSLSYKVHKSTLKWAPVFNMTILLSIAIISCLIRVFSVIRYESIIHEFDPWFNYRTTQYLVKEGPYALWNWYDSESWYPLGRSVGGTVYPGLMMTSGMIYWVLHKLSIPIDIRNVCVFLAPIFSAFTSLAAYGMTKEITKRSEAGLLSALFVAIVPSYMSRSVAGSYDNEGVSIFALVFTFYTFLKTVNTGSILWGVYTALAFFYMVASWGGYAFIINIIPIFVLFLLITGRSNQRIQVAYNVFYILGTLLAMQIPFVGFQALHSSEHMASHAVFLLINFLNFFSWIRNFVSKKAIITLTKFLVVFLVFSILVGVVILTLAGKTQWSGRSLTLLDPTYAKKYIPIIASVSEHQATTWSSFFFDLHYLILFSPVGLYYCYYKPTETKIFGALYTVLGVYFASVMVRLLLVLAPAVSIMAGIGISWAFRKFAKSVRSFILPKIEKPKKYRIPPEISIIGLVLLGYYCSIYVLHANFAGSEAYASPSIILSSRDRQGNRNIIDDYREAYYWLRMNTKADDKIMSWWDYGYQITGMSNRTVLVDNNTWNNTHIATVGMAMASSEEDAFEICEKLDVDYVLVIFGGILNYSGDDINKFLWMVRIGGGVYPHIKEEDYYGKGTYRVDQYATETMTNSLMYRLSYYRFDEVQTAYGQPKGYDTVRQAVIGVKGYKLRHFEEAFTSENWIVRIFKRKQRENRDGVAFTSKQTSQFTIPKETIPVTKFSKKANIKQRFTLNFNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6S0</accession>
    <name>A0A8S1L6S0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0320236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6S0"/>
    <dbReference type="OMA" id="TFIKFAF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="241" mass="28661" checksum="F7A02FB3CE2802FC" modified="2022-10-12" version="1">MSQTIRRTKFRIKDVIDSNLNNQEQSAQKVIQTSYIKQNVDDFNSLKQTLEDRKLTFIKFAFQQQELIEDRNLGPVLSGLCRHQNVGSMSVSELIPKSQIHTYLLQAQQDRPKRVLKRFAHWEWDKDQGRDKNPMNYQFTQRNEAEIKSKLKKKDGEISLRRRILQKWQKHLPSLTGLDEFGKQFTMDHSSLKKPFSLSPTPKQESYHQITNWLQELEELKLDNERVKKKLERTLQSLHRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L746</accession>
    <name>A0A8S1L746_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0310266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L746"/>
    <dbReference type="OMA" id="FKFDQTY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="24211" checksum="02EF9EF4C5734CDF" modified="2022-10-12" version="1">MSNYKLLMKMPQSKLASKCLALEELLQRYYEFETAHYDLQSKYQILLEKYESICKKGQQINDLHTTASQSNKKDKSEIEIGDRIEIYALEDIETNQFIKSLQDQNLRMQEQLKQSQIEYEKLKFKFDQTYSSPEKDNQEGIISTFKCEISRLNKLVSQLRLEISNSKTREMDENWLRQQLNQQESLKQGKLLSPQYESNEVTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9Z2</accession>
    <name>A0A8S1L9Z2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0330144</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9Z2"/>
    <dbReference type="OMA" id="NICYIAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="3">
        <location>
            <begin position="43"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8062453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="350" mass="39817" checksum="9C7EA2CDCCEBACD2" modified="2022-10-12" version="1">MFLLNWFGGNSNQSGKNKKKKKKQNVVQNLNKTLINPMKEIKLFGHSKMGYGPKKTECQDSYCTMERFTEDCYFFAVYDGHGSSGKEASQAANDYIQTFLEKNPKRIKALQNDKQRESFLKSAFKNAEAKLRSSGIDYSNSGTCAISIFVVKNMCYIANLGDSRAVLFRQTAKEKLAIELSYDHKPTRPDEKERIIRCGGKIERLIHDGQPVGPYRIWADDEGPGIAMTRTLGDLQAKKIGLISEPEVQHIELTKQDKFVVIGSDGVWDVMSSAEVCGFVLKHEPKESVAEAIVTECRSRWDEMNKQKKTNSRIGDLPYLKFGCDDITAVIAYFTFIDELEDNYFQQQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LAP0</accession>
    <name>A0A8S1LAP0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0340233</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LAP0"/>
    <dbReference type="OMA" id="YPFENII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="807" mass="96882" checksum="11A08E40101EF90E" modified="2022-10-12" version="1">MDLSDHKYNESFTQIELSVGTLTNILYNYEFFKRISRFLSLKDVLNIGQTCSILNEVVEKHQEFFGEIYYKLFLNEQLEMTLELNYPDILRRQYIGQSNFDNSFINWKNQLIKMIQTKQTLKFPFILFDVVFPKPILTRETIGIYCESNYQIMLAQRLELEQKGFDKLFDYQFIQNESMNKLKQNKQQILVESQDKLQNNEKLKRQFLQYRWCLQNESFEFQQFNFQNIDIVEQDYENKIESISDEQICIFNLMEEFYTSLECYLIGLQKYFSLFINVNTINSIDLLCEYVMYWQVYSNAIMDLSTFIYPFENIINELHSNLFPNYPQYPQFSVWRVMTKMWIKYIIRNQQLSVILLQCFIRLLSAQRQQMFKQEFDQGVNDDYENTESFLITYEIYDNFLLKQRNSCKDQFQIDTTHLFYTEIVDLLRSFCRCIQDISISEVSVHWVGHKDCCYEEFYEQLSEKIQYETSLYYDETRQIFGSNIISFIEFIKFDKEFLQQIIPEPLLFKIENIQREHINTSLYYFLEVSYLQKFVQNNKDQIILVYKNSKPKSPKQERISITTSLNNEHLETQGDSNLNEAQKKNNLCLENSNLDLEELLIKEKNKNQSDPLLEIIKVALSQMNLDQLSNFDNIDQYGSQKDLFDFNKTPKIHRAFSNTKLNAEQTEVPEEITKAAKQYLLADAQFSKLYQVFIEYSNNYDKILIQVITKDRDIELINDDREVPRILPEYLQNFYYVTNYLTYSVLEENIIEDQEEEFQDQDLPLNLQKNSCKLTCIYIIKQKIQFKFNIITQKKKILIVIGMQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDZ8</accession>
    <name>A0A8S1LDZ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0380207</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDZ8"/>
    <dbReference type="OMA" id="VNYLMFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="262" mass="31306" checksum="EDD93A2C5823879D" modified="2022-10-12" version="1">MSCQRITVTKRGVELQEQLKEVLKKNQKILKLEQQRDSSGDLLKRQNIGKEQFGGYIDLMEQRMNSPGKIQVFLNQRFSNQPKFIQKEVEKVSQKIYNYGNSQFKSHHSLPSLDFRQVQHLKTKYNDGFWNSQNLSMSGNTFYKPKDEEVKTRKVNYLMFQEAKIERMITKYDKFNEKAQFIPSFEKGKQKNSNLATELLKAMEKKDTVTRVESIQEINQKHAQNLDLMEYNSQRRERFHQTMTELHNKKYKRHWDNLRVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LE62</accession>
    <name>A0A8S1LE62_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LE62"/>
    <dbReference type="OMA" id="CFCQICS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="112" mass="13152" checksum="A9A260151329EEBD" modified="2022-10-12" version="1">MQINKEQSKDKIIQDSKNENSHPLINIICDNLKNICTLTLQIKAKQIKKKSLNLKKKKNSKTKISKRDKKTNQCYCQTCPDFKLLEYLSQLSAVENLQHQLDKVKELIYLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFD7</accession>
    <name>A0A8S1LFD7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFD7"/>
    <dbReference type="OMA" id="HIWEAGI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF132">
        <property type="entry name" value="PROTEIN-LYSINE METHYLTRANSFERASE C42C1.13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="217" mass="25428" checksum="DA028163FD1B3B66" modified="2022-10-12" version="1">MNQQFQIDDIILAPGLSIKIRNTIQFKHGQDGFHLWEAGIVLTRYVYYNIMPSKQIFMDLGTGVGITGILISKYTRVIMTDYNQDVLDNAWINVKLNQSPCILMQLDWRNHNKISFQVDLIVGSDLVYSGAPLYDLYQTIVKLLKQNGIFYLIIPSNRMCTSEFIDIMNSEGLFEIRKELLEDEKYYQPCLLDREQSHQLYPGLKELKFYMYIFIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHG1</accession>
    <name>A0A8S1LHG1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0400190</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHG1"/>
    <dbReference type="OMA" id="SIRNSCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000740">
        <property type="entry name" value="SNF1-related protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="29"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="9">
        <location>
            <begin position="302"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="427"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="570"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="578"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8067257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="596" mass="68980" checksum="E525990AD4064B9E" modified="2022-10-12" version="1">MKLFNSRKSIIKEQDQESDKQRQIGDYLLGWFKTLGLGTFGLVKLAIHQITQEKVAIKILEKSKIIDVADVERVSREIHILKLIRHKHVIQLYEIIETKKYIFLVMEFCDGGELFDYIVKHQKLSEIEACKFIQELISGIEYIHKLNIVHRDLKPENLLLDYQKSLKIVDFGLSNTYKQGEQLKTACGSPCYAAPEMIQGNKYNSLLVDIWSCGVILFASICGYLPFEDVNTSALYKKILNGEYKIPNFVSPEGTSFLKGILNINPEKRFNLDQIRSHPWFKLYRRSHPIPPGIIIGYHRIPIDNNIVQQLKERGFNEDYIKICLDANKQNNVTTSYFLLMKRHLMNGGISTADINSVHFDQKLLEPIQRVQKAPILGFLDESMLKTLNSNRSQSNQSRQHKKMHTQQNRGHYYLQDEKLNKSIQLKQSNRQDSIDSDEEFSKPITSNVKKQKVRTESNYNADSQLNQTTMIAGKRQSISPANANLILQYIKQQKKLTPTSIRNSCQQKPNIFSLNYQRNVQTIYDHDGLKIMNSNKQQSNEQKRSQNNQWMPQSLMNPYQLLLQLNSRKGSRDLSAPHSTKCANNPKSKSINRQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIE4</accession>
    <name>A0A8S1LIE4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0410261</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIE4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="3">
        <location>
            <begin position="6"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="135"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="207"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8067978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="353" mass="42267" checksum="4A5B1C85F296C46B" modified="2022-10-12" version="1">MNNKMVNNQKQQQCQEERIDRKQMFKDVDHSLRILTDIQLPQQMSKSIQDAYEKGEIEMKNNIFDNIIKQNIIGFSFDIYNHYIIQCILEKGLSEHKSYILRLVVENIEFLCYRKYAYKIVQSCLIQYQNNQIIQFIIDNLKKLQFDQYGNLIINTLLDTIQNDEQFAQIINKLQIDKIKYHQYGCVILINMVTYTKSNHVDSIINKLIQESIQLSKSQFSNYIIQKMLKERTIEQNKLLINEYLIPNFIELSCNKFGSNVCEIMVNKSLAYQLQNLWNLVIKQNLKLLLNNEYANYVMQKYYSHILIFNYILEEYQQSLYELNKKQLLNQNGKIVYRFVNQCNQVVQLNQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LMR5</accession>
    <name>A0A8S1LMR5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0430165</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LMR5"/>
    <dbReference type="OMA" id="QWRQSKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051581">
        <property type="entry name" value="Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524">
        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524:SF17">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_48, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="62"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="177"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8069037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="328" mass="38844" checksum="39586650924E8941" modified="2022-10-12" version="1">MKQAQPFIPLAVDDNKQYDIKNVLQKQIKQLKPEEYHFFSFLIATNHVFEQLTNCQNKLNNDPTFSLWKLFEFFDVNGDRKLQLDDFIITLQKLDILLKREEIESLFSTLGGEDDNIISYAEFANLFKFSSTPQWRQSKLPSEGVLNQDQQKLIHQIFSLYAKMERSILHLRTQIGSDIQQLENFFRKLDQKQKGYLILNDIITYVKSMGVQVKGNDYYRVFFMLQQYKSGRISFNEFLKRFSTKEAYNNYLQLLDLLIHPPQLAQRQTIEQQQAVLQTKIEYTTNLESKHIQQNEIQSKISQQSRFSQDLDDSIRLPGYTRYIFGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUW7</accession>
    <name>A0A8S1LUW7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0440252</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUW7"/>
    <dbReference type="OMA" id="ECCVKKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="30"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8069815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="445" mass="52448" checksum="98E24B27931327E0" modified="2022-10-12" version="1">MQNKDQGLFKTVAINNIVYLNLEKGLVYIPNKKVGEDAGNNGEIFKGYIGYNTDKQIECCVKKFSIPTYDKQMYEKLHQELIFGRQLVHKNIVKFYECSRSDNNFYMFMEYCNGGSLAERMSLGYEDSLMSTKIFSEVQAINIIQQIIDALIYMKELSYDGKVGAVHRDIKPDNIMFQNDIPKLIDFGMSRFMDKQTITHYKGSPIYMSPQNLRFEKYDLEKNDVWGIGIILFQLVEGIYPWRMALNSLEDLQKAQEKIKNNIQFKNKVSEELQSLIRHMLQYDEQDRCDWNQVTNYQIIKHQNHLQINTINRLVLNYELFEDLQTKLNIEKLSKCFLDLTLTTINNLKEFLPKNSSLEKKIDQLYIKRILQKRKFHTFKSERALKYKLSQRLVKYLEQLILDTIDQVQTKNVQLVCQIIISLYKTDAEFKDELPLTSLLQLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0S9</accession>
    <name>A0A8S1M0S9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0480254</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0S9"/>
    <dbReference type="OMA" id="HYIITDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000475">
        <property type="entry name" value="Calcium-dependent protein kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="44"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="342"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8072142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="440" mass="51515" checksum="AAE3FCB44E6200CE" modified="2022-10-12" version="1">MGNKIMKSQAEFEQDYQRKQEQKPSVLALNLLIKKKGRVSTEYQFLTPPIGKGQYSEIRKVINKKTGIMRAVKIVQKNANEKEEERVTSEVNILEKLDHPNILKINEYYSDDRFHYIITDYYSGGELFSKIQERRTFSETQAARIIRQLLYALNYCHLHGIAHREIRPENIMLEKSSEFASAILIDFGMCQKISHKMHSSVNHPYYQAPEIQLKKYNEQIDIWAIGIITYILLCGYPPFGGESNRKIIDNVLKKPLVFEEQDWNKYSNESKEFVKKILIKDPSQRITIEQALNDPWIVKNFELQVNDGQLKRVLTNLVNYNSQSKLQEATLKLIVLYLASREELSELREIFTYIDSKNDGYIDVEELQTAMLKIFDFETTERQINKICGEDETLSYSQFLAQAIDKQAILQKSKIETAFKLIDRVLVKSNIIECFWKYQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M360</accession>
    <name>A0A8S1M360_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein pelota homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0440029</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. In the Pelota-HBS1L complex, pelo recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M360"/>
    <dbReference type="OMA" id="DDLWHLK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.870:FF:000002">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000059">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8069444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="422" mass="49449" checksum="A195C0500958FEB9" modified="2022-10-12" version="1">MKILSKKIIKGVGFIKVIPEEDDDFWHLYNLLSVGDIIKTSTHRKIVKESQTGTKVAEKRRIFVFLKVHEINYWADQYLLLSIKGRNCQESHWLSLGQFHTYEVELNQQIVIYKENWDRFHYAILDQIKQQQTQSEVAAFIMEEGVGHLCLIGSATTRLKQKVEKQITKKRSSNEQREKGMEEFFRACLAALEQDSLFEQVKCLIVASPGFVKDDFYVFLRNTFQKEEKYRGQQKMLDKIFLVKSSSGYLNSLMEILQDQQVQQRLENTKAMQEVMILEKFFQQLRIDITKVAYGKKDIEFAHSLGAIQTLLISDKVARAKDPKVRRQWLKVIEEIKNKGGEVFIFSSLHPSGKQLNNYTGIAAILYVSVQFDYEDDEEEEEEQVDQQIHQHQHDQDEFKMDIAVQKKFEEFGIEDGDDDYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M516</accession>
    <name>A0A8S1M516_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0480031</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M516"/>
    <dbReference type="OMA" id="CDWREAR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000574">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="219"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="478"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="67"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="549"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="93"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8071746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="569" mass="65203" checksum="096A797150424AEB" modified="2022-10-12" version="1">MQKTNFKNLKDLLQNKYAYQEATSNSRTSSIAEVLQNLKKNKDQTPVSIMKEEIINFTKAIGFGIQAPSSKSKSKEKQALPKSIDKKPILSNVAPQSKGNSRKNSSQSRNTSAHSIINENTNRSIAKDPKIPKGFQTGRLRERSEMNSILDQQKIKILNQSQDQQKQNLNNSNVSKQGTIDLVNLLKQQQNQKQQSINETINESKTFGEEFCGLNKGHFIYNYVIGKGGFGKVWKVELKKNRQMFAMKEMMKSKIIAKRSINSVMNEKELLSQLRHPFLVNMCYAFQDRDNLYLIMDLLTGGDLRFHIGKMKRFKEHQTKFFIACIVSGLEYLHNNNIIHRDIKPENIVLDKRGYARITDLGIARKVRPDNSQDTSGTPGYMAPEVMCRQNHGIAVDYFALGVIAYEFMIGKRPYNGKSRKEIRDQILAKQASIKKEDLPNGWSIEAMDFVNKLLQRKPQNRLGFNGPSEVKNHPWLKGVPWEKLYNKTIEAPYLPINVEDMYRQQISDDGEESQDELIKENQLLLRKNSVQNLFAGYGYDCNQDPTYKGTRSTASTHNTSQQNETIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5M7</accession>
    <name>A0A8S1M5M7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0480116</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0630149</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5M7"/>
    <dbReference type="OMA" id="RINEWMS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21455">
        <property type="entry name" value="DLC-like_DYNLT1_DYNLT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.40">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005334">
        <property type="entry name" value="Tctex-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038586">
        <property type="entry name" value="Tctex-1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255:SF4">
        <property type="entry name" value="DYNEIN LIGHT CHAIN TCTEX-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255">
        <property type="entry name" value="T-COMPLEX-ASSOCIATED-TESTIS-EXPRESSED 1/ DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03645">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="124" mass="14345" checksum="5A8CCCF7E9CE28B4" modified="2022-10-12" version="1">MEENQNLEENTYSQEEVDRITQESIESVLKEVQYDESKVQGWINAICEKVTQQLIELGKPYKYIVHCMIMQRNGAGAFVTTSQWWDTVADGQIIISWPKDKQAKQEQQKNTLHCICSVFAVSMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8S5</accession>
    <name>A0A8S1M8S5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0560188</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8S5"/>
    <dbReference type="OMA" id="KHKIGLM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000023">
        <property type="entry name" value="Probable uracil phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050054">
        <property type="entry name" value="UPRTase/APRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315:SF4">
        <property type="entry name" value="URACIL PHOSPHORIBOSYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="10">
        <location>
            <begin position="58"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8076537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="272" mass="30741" checksum="04D49CCA63BE3770" modified="2022-10-12" version="1">MFRNLIKIGGCLGLLSSSNVLCNQIKGKNQDDQLPHYHFLNDHDLQKLQKKHSNLSIIDKSYTEHILSVIRDVQTDIVDFRKNADRLIRILIEQAISQIDKKKHIKQSPLGYYDAHELKFQDEEICFVSILRSGNAFLIEALKVMTGASIGQILIQRNEETSQPAYFFQKLPQNIKDQQVILVDPMLATGGSASMALQILKNHGVKEENITFLTLVSCEQGLGKLFSEYPKIKIITAQVDPILLKNINYLAPGIGDFGDRYFGTVKTNKAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9F2</accession>
    <name>A0A8S1M9F2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Opine dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9F2"/>
    <dbReference type="OMA" id="HPARYYS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051729">
        <property type="entry name" value="Opine/Lysopine_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003421">
        <property type="entry name" value="Opine_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38015">
        <property type="entry name" value="BLR6086 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38015:SF1">
        <property type="entry name" value="OPINE DEHYDROGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02317">
        <property type="entry name" value="Octopine_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Opine dehydrogenase" evidence="1">
        <location>
            <begin position="189"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="403" mass="45912" checksum="904439DF66E2634A" modified="2022-10-12" version="1">MIPTNIVVCGGGNGSHATVASAGRFENIQVNVFTRKPQDWKQEIVGLTKGSPWESLGNFVGKINKVSNNPQDFPGAKLWIIGGPAHVHHEILQKIAPFVTKDSFVGTLYAQGGFEWMCRSVFGDRIKTDNITYFGLYNIPWLCKIFKYGESVRIIGPKTKLQCALSNLSRKEELFLLLENMFQIPVLQAPNFLTLTLTPSNQIIHPARVYAVFRNWDGKQVFQPSEVPTFYEDFDDFSAYMLQILDDEIQAIKREILKRYPHFNLDLIIPIKERIIQQYGDQVKDKSNLKTVFRTNAGYATIQIPTKPVQGGVQLNTEARIFWEDIPYGLCILKDLSEMLGLQTPGTDKMIEWHQKFMNKEYLKNSRLNRNLLKETGAPSKYGLDTLEKVLGIQEVPQQIPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBB3</accession>
    <name>A0A8S1MBB3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alkylglycerone-phosphate synthase</fullName>
            <shortName evidence="2">Alkyl-DHAP synthase</shortName>
            <ecNumber evidence="2">2.5.1.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0580258</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the exchange of an acyl for a long-chain alkyl group and the formation of the ether bond in the biosynthesis of ether phospholipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a long chain fatty alcohol + a 1-acylglycerone 3-phosphate = a 1-O-alkylglycerone 3-phosphate + a long-chain fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36171"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17135"/>
            <dbReference type="ChEBI" id="CHEBI:57534"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:73315"/>
            <dbReference type="EC" id="2.5.1.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycerolipid metabolism; ether lipid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.26" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBB3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008609">
        <property type="term" value="F:alkylglycerone-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025650">
        <property type="entry name" value="Alkyl-DHAP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46568">
        <property type="entry name" value="ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46568:SF1">
        <property type="entry name" value="ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="3">
        <location>
            <begin position="12"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="113"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="178"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR625650-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8077728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="269" mass="30574" checksum="4B13BB20EFED1D38" modified="2022-10-12" version="1">MQEIYELRHGKLSRIVDCVIYISSHTKAETLVNLASENNVKLCVYGGGINVTRALQCPKEEKRMIVSVDMSRMNHVRQVDRKNMTDIVETGITGKDLEKELSRYGVACGHEPDSVEFSTLGGWISTRASSMKKNSYGNIEDIVLSIKVVTPTCTLQQSFDYPRFSSGPDLNQIILGSEGTIGIITEAIIKIKAYLNLKYVNMNLFYSIILLQVMNLCINYPEVKYDLHQLDQLIIINFNLYSFENYVSQQEGRIYGQNKKILYNSVYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCQ4</accession>
    <name>A0A8S1MCQ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0540223</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCQ4"/>
    <dbReference type="OMA" id="NYTTKTR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="216" mass="24250" checksum="D0835A8B5306E706" modified="2022-10-12" version="1">MLTQSILSVESSKVYKPKNSQIGLLSTAISKLGDSNYTTKTRESTNMSPVRMRLFRDSSPKQSKGLQGVVVGEYHALTPAHIVFSSIHKSTSPIRSNRNYNESPQRAKVRLDRFKNWDQKLGLELQIQNTKFTRLNNELSYSSYQSHHLSTKSSIFLGNNQSRLGFGGNQSDIRLLSAKLNAIPPTQIGAFSAGHNHELADLQQSLSRILKQSRQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MG62</accession>
    <name>A0A8S1MG62_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0520200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MG62"/>
    <dbReference type="OMA" id="QYQYLSK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="124" mass="14581" checksum="C00CAFE57380B304" modified="2022-10-12" version="1">MRVVSETKYYKVLAKLYEKCASLSAQLDIYEFDSPKAEQFEQSENSDDNIIKRTQNKRKQKYIPNTIIKLEEEQQKQQYQYLSKNKKTISQNNNGKSTKIKKKIKKPKNYDFSSSSSQTSSSYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MM19</accession>
    <name>A0A8S1MM19_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0620031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MM19"/>
    <dbReference type="OMA" id="IQINQEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035723402" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="28022" checksum="A2F45FCF5A01DCF5" modified="2022-10-12" version="1">MKLFILFFIFSCYSSMNIEVLVSKLEIQEILKAYLMKISIQNLSIQINQEMSLVFGNLLTKNLAISDLTFNEDLIKIDLIQDPKSMKVFIWLDSFALKLDVDINTKVGLNYQTQLNLEVYGLQLEATLLLQKNQISLQNEKLDNFSIKSMRLKQGSGQIENIIKGALNQLINLFKNLMNQKCTNKIIQFFKQEKWKKLVPSYISQIKELEVMDSENLQLKFSLDNSEIVSKISSIRSTQDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNL0</accession>
    <name>A0A8S1MNL0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0670059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNL0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="71" mass="8823" checksum="2508AF5BA5623DD0" modified="2022-10-12" version="1">MKFVFKILAKYVRKINARQFKMKLINLIPNIIFDPKLVYRRKTYEIFTKISNSQIYYMNFRKHQEQQVAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQ62</accession>
    <name>A0A8S1MQ62_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0570191</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the IPP transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8077054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQ62"/>
    <dbReference type="OMA" id="WGLHLKS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF60">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8077054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="430" mass="50846" checksum="F8D17497E5D33CB0" modified="2022-10-12" version="1">MNNNGFIVYIIGTTAAGKTNLSLNLGLDNYEIISCDSMQIYKDANIMTAKVTAIEQTIKMHHGIDLLELESEGFNRKQWKHMTINKIEEIQSKGKIPVLVGNTHNFIESILFNQETEENQIQQAAEFHLDGKEPFEYLKEIDPLAALKFHPNDHRRIQNSIKYYQNIGQLQFDQLDHNSEQFKLRSNNFLIIWPKWRKNEDLKIKVTERIQQMIEKGGTEEILRIFERLENKNNKLGGVLKGNGYCQFEKVYQYYKQSGLQYPNIDENFQVLLKQGAEKLINDTMQYTKKYIQWIKNRILCNSQIDIIANRLFLLEFDSIQTFQEQVIIPAQKIYNLFYQQFQDDQLGDEQFKISQQNLGNIKIITPEMIEKYKQIQKQKSRNSWKKQVCELCNKTMNGPFEIEQHFKSRFHKINVLKDEQLQKKKQKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MRU3</accession>
    <name>A0A8S1MRU3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0620230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MRU3"/>
    <dbReference type="OMA" id="IMGLCIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336:SF3">
        <property type="entry name" value="GUANYLATE CYCLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336">
        <property type="entry name" value="OXYGEN SENSOR HISTIDINE KINASE RESPONSE REGULATOR DEVS/DOSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="280"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="446"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="34"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8079846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="590" mass="67633" checksum="7D561E612BBD95EE" modified="2022-10-12" version="1">MNKQQELNAFENGKKFKIKTERSTQIASPKNKDMDTQEDIVKKLTDNYKMQQKEIDKLFITIENKEDQIRKLQDFVKLQQKEIDRLTHINEYLMQANQTKLSILRDERQLNQGGKTNQKFKTHKSLLPLEKKSNKITEEGNTQSVKLFRRQTFRNPTLVRFHQDDGDTFKNDRNTSGASNFYDILQQEDCFRTNAILNIQLSDEDATTQYNIDGTVSLMKVLLESEENFCDIIQSISAQKLSFLYDKFKRIMSDHQQLFILVLRLKKIVTGALQMNSSVLLDDALQTIIDKCIDCLECDRASCFIVDQAKKELWTRVAKGTQTTIRLQIGQGLAGFVAQNRIILNIEDAYRDQRFNTQQDVKNNYKTKTLLISPILDGDKCMGVLQCVNKSNGYFTKDDEALLQIMGEFSKSVLKNAMNHDAQMLIQNKLRHIIKTGIILQSKQDNLIDLILSAEERLRSLMNVDQAKVVYYNQVLSHINRDGKLSQIDTLLGIMGLCIKDQQLVAVSNCYTSPIFNPNIDIETNMPIICMPLKTQNHQIIGAIQVINVKGIGNISSNSEAKINSIDLDMLELFCQQCAQSLQQCKQSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MU87</accession>
    <name>A0A8S1MU87_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0700204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MU87"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="15646" checksum="7D436E1355E7BAD5" modified="2022-10-12" version="1">MKIVLQIFWTIYRFVEKLSILIVIIYLLAFPLLSRIYSLIEDVINYQYKSIQLKDHQQSIFSFLPFIQFTLFILYHFGTNYDVLKNNYFETYHFINEKLCYQSIVLPCVFFFVDHQKLDELFHIYAIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MUC3</accession>
    <name>A0A8S1MUC3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hydroxymethylglutaryl-CoA synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0600109</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MUC3"/>
    <dbReference type="OMA" id="NKSTRIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00644">
        <property type="entry name" value="HMG-CoA_reductase_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000008">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.770.10:FF:000006">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004553">
        <property type="entry name" value="HMG_CoA_Rdtase_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010122">
        <property type="entry name" value="HMG_CoA_synthase_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01833">
        <property type="entry name" value="HMG-CoA-S_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00532">
        <property type="entry name" value="HMG_CoA_R_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="5">
        <location>
            <begin position="3"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="6">
        <location>
            <begin position="178"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8078544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1205" mass="136461" checksum="9C23B1A14E77124E" modified="2022-10-12" version="1">MKPINVGIEAIEIYFPKTYVNQAELELFDNVSQGKYTIGLGQINMAFVRSFEDVNTMALTVVTNLLEKNQINPALIGRLEVGTESLLDKSKSTKTTLMRLFGENTNIEGVTSINACYGGTNALFNTINWMQSDAWDGRLGLVVCTDIAVYAKGSARTTGGAGAVAMLIAPNATFTLETIRTTYMKDNYDFYKPNFQSEYPTVDGQLSIQSYLSSIDNCIQQYFKKNNNLDADFYCFHSPFHKMVQKSFLRVKLNESFVQKVETGFTFQNFNEKQSQMLKFYQNDWLNKAFPSCLLSRELGNIYTGALYAGLISLIDTQEDLINKRIMMFSYGSGCAASLFFLKCNKSTRIMKQKMNLQERLQQRIRISCIEYDHIMQQREINYNKHSQQYQPKQVDLYPGTFYLKSIDDKYRREYLVHKQLELNQTSDIIVIDNSKSINKIQQIRDQITNNNSTNDQNTQQSQKQYNQLWTGFYKKTIQQRLDQLEKTMNVNVEPFKDGGLSLQNANLMVENCIGKISYPLGLGLNFLINGQCYSVPMAIEEPSVIAAASAAAKTISEAGIGFQTYSSRPVMMGQIQLLDIQNFSKIECLIDSNRQTIINRGNQACQSMVKRGGGIEDVKCRNLNKGQCSVDIFINVCDSMGANLVNTVLEFVAPLITEITGSRIGIKILTNLCMNRKVTAQFSLDIDKLNYKSLNGKEVAKLMLEAYQFAELDIYRAVTHNKGIINGIEAVCNATGQDARAVNASLHGYASINGQYKPLSSYKIIDNKFIGELTVPISVGTQGGVLPQNPLYSQILQILDYPDSQKLAEIIVSVGLASNFAALRALAVEGIQKGHMTLHARNIAIASGIPEHLVDEAVLFMKNRNNFNKQTALEFLKAYNVFFEIGKAKGKQMKAFCTFTGCFDLIGTQEKVDLHVVFECDQNKINLNYADDSAINRQIFGLKGQEWLKNLFSVLNTVKINKEVELYQSNNLTSKLKLLAILINLLCFNMLSLDFDRSKNFILNQKPCLGGAISLKYGLSLMNELIHIFEYNIDQFIGFPQLRQALMTELNNIILAHIKSFELLQQAKQGYFNCDEFLSSRQKRLCCTMMLLCDCISLKNFSPSIIEEIKLLGRVIEIQMTLTRDTQKWNKSTLSEPNMYTYWLIANQKLILGDNKDILTSFYNEHNRLLLSLIQELEKNISHQLSHLKDQAMNAIQLFYKPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWU3</accession>
    <name>A0A8S1MWU3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0720216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWU3"/>
    <dbReference type="OMA" id="SRIKTVP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8084700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="101" mass="11986" checksum="303D6362BD32E9BA" modified="2022-10-12" version="1">MLLQQQRKKIQPSFVAFYEFKIQEIQSYPSQRVIITEQNIKNYANLRIGTYSPISCKSSRIKTQPQVKKEKIIPFEHDYNTFYRTVSSQRNLLNIISGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWU7</accession>
    <name>A0A8S1MWU7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0660008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWU7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="271"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="521" mass="62553" checksum="6EB8A38C6923F450" modified="2022-10-12" version="1">MFKPKMIENENNLHCQNKHGQPVHLVVLDPKLDKKQRLLCTECMENFESDAKTIGFKKIIQIIEDNIKKTMSDLEGITQQTVQQLQICNRSIQELKAYFMKLFDSLISIVEDWTQNLFALTQQSKQYSFYDELDSFIKKQNNINDSNITLINNINRSWMAKLLKILNQYIQDKDKVNFNEQNKNLQTSFVQFIHKKVKQNQSQLINLLNKVWDVKIQFLILLGQLWYQLNRKILKYGAFQTEKFNQQTYCDTTIRCWKQQDSSDWISSQPYKQHTDFVICLLLNSNEDLLFSGSDDKLIKVWKVDFDQNILTYIYSLYQHYKEVFALSLNQSETLLVSCAGGDNEIIIWERREQDKYEFKNFVKQSIECYGFKVKFIKDNQFIWISGGKVINKFYVFELKEGIFQENQEKTIQLFRNNYIFDDYCFPIMYNQEKNLIIVRHKTHIYIFKEMKDGKFKILDYLNCNTYFIYGTITNNGQYLIYGMINRKDFQHMNYQIHEQLCLSQYIILFQQYNQYMQVHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MYT7</accession>
    <name>A0A8S1MYT7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MYT7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="144" mass="17750" checksum="97105AF0FD66DA2F" modified="2022-10-12" version="1">MLQEYGLTQEQLTKQIIIGLAHPQHIQFFQQIITINEYKIFGKQMQQVFLFQYQLYTLILYFQLINIIQNRKIQQFIKQQQQNINNQQLAPQFDQQFELNFFKIEKEKAEIELAIQKKQKMNRNILRKHKMRIYVQKNNRIKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0B4</accession>
    <name>A0A8S1N0B4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0680003</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0680004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0B4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="141" mass="17224" checksum="84BCB9E8092BEB94" modified="2022-10-12" version="1">MINLKKVAKLNFQSFSKRCINYKRLGFKLFFRRIISIKNIRHQNNQYKAQQLTQPVNHDHRDPIDTALSRAIHRRQEMYDNPNEVQRKRYKQIRHMHWLTLQQKPFWLIRKKPQILILKVQRLILNVNLQHEQNSSFIGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0H2</accession>
    <name>A0A8S1N0H2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0690001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8082883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0H2"/>
    <dbReference type="OMA" id="NDPGLCN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18888">
        <property type="entry name" value="NUDIX_ADPRase_Nudt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000152">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF1">
        <property type="entry name" value="ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="42"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8082883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="197" mass="22977" checksum="4146AEB6C0D4FEBE" modified="2022-10-12" version="1">MDFNTKVDEQLIYEGKWLHYKFLHFQVNGHKKVWEFVERPPGHRGGVEIIPIIKYKDKHSQLIIIANFRPPIRKFCLEFPSGLVDQEGTIEENGLREIKEETGYTAQIGNYQYNGVRIRNDPWKSTENEVSIIVDIDGDSEINVNPKQQLESDENIIVYKMNMGKTLADEILQLAKEKDYEISGLLWFFALGQQFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N3W4</accession>
    <name>A0A8S1N3W4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0800049</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N3W4"/>
    <dbReference type="OMA" id="RINNSCD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="5">
        <location>
            <begin position="220"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8088027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="449" mass="52439" checksum="8A9B463D286F88CE" modified="2022-10-12" version="1">MGGSKSKSIKKHAKVAFSDSELEILEVYFELLKCHDSLNGYLTQKSMVPEFPENPDFSIKLYNWMRERCKNQQIDYTNFVITLELLLKEQQEYYLSDYKFRVLEKFELFALISLGCLENEKEAINRFQVSYSQGSQVIKELLNMYYFNKEVTNSQRNDLAARSVANSIFDQKASLPFNQFIGQAKQQLIYANKMCKQYFTKKFIDPHLKYGIPTLTSSSFILNDQILALLQMSSPDFNRIKQLDLKFSSSVGYDDLEHITEIIIESQQPLLFIFRNREDETQNDTFQQQIFGAYISIDQTIETHFIRKQQKDPMGILYNPNDQVSLYFGDEKSFLFSLLPKYQLFMSTFDLKSKQCFGYINSRAFNLKVPQPYGLGFGGDGKGNFRIWIDENLKGNATNRINNSCDQTYEMGYLLEPHIEFLNLTSIEIWGAVFEEKDMRPSNQNKLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N810</accession>
    <name>A0A8S1N810_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0820087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N810"/>
    <dbReference type="OMA" id="HKSNERN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="230" mass="27102" checksum="D88E32B58487CF94" modified="2022-10-12" version="1">MFKLRTHTVKQWLLQRFRKSVNSQYITDPQEEDYKQELAELFAKFDQEGKGKIQKYELLTLLNKYGININEQELTSYIKQANSKSGRFITTEQFKQCVLSEKSKLFLKKLIHKSNERNPQNYYPTEITKVLNQVHYLSERSRLLSQIEDKQIPVEQKLQPLSKLMNLSREITITEQRPQSKCPLRLPRARSTCTTNYNSLSTAITVPSTSNSRFGDNRISFRKHPTQYCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBP0</accession>
    <name>A0A8S1NBP0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase class V domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0760197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBP0"/>
    <dbReference type="OMA" id="TGNCHKW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092:SF2">
        <property type="entry name" value="HERCYNYLCYSTEINE SULFOXIDE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092">
        <property type="entry name" value="L-CYSTEINE DESULFHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="2">
        <location>
            <begin position="30"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8086435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="385" mass="44584" checksum="B6FA728C6DDEA8B2" modified="2022-10-12" version="1">MNTFGQNIKEDFIIENGFVCVNHSSFGFVPKQVFQLRIQHYTKFLQNPDRFTKIDYPKYTIEIRKTAAEFLNADFNQCMLTSNSAEAFNSIIRNLGLTENDTILYFSTAYPMVQNTIQFMTSNYNVKQNRIELKRQHLEKQKILSLFEKQLKTQKITIAVFDNISAQPSLIMPIKELISLCKKYNVISIVDAAHGAGITVLDIKDLDPDFLFTNFNKWAFCPSGINILYLKEQYLNKIHHNTISVNYGNGLAKECEFSGTKDFSLSLSLIDGVNFIKKYGLNQIIKYSEDLAWEGANLVAQIWQTELLVNDKSMHSAMVNVRIPHQDQNYCRECQKKCFEKYNVFLVVFKYDDLNWSRLSASLYNTIADYEYAARCYLKVLKGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFV0</accession>
    <name>A0A8S1NFV0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0820151</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFV0"/>
    <dbReference type="OMA" id="KESSCNR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000465">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="168"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="201"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="234"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="392"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8088941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="702" mass="81360" checksum="A29248BDA9FD6F54" modified="2022-10-12" version="1">MNSHRKLTLSEYQTKQRQIHEHRSPKSNKFHTPQQKININSSKILITPKQLKLKPFSDRSISINLNQNFPFPSKISKLTKLQSLDEETKQCESKNQQQTIEQKSPKQEGIDKLRSQLNEQFLKESSCNRAGACLQFLEPTIVHNMLNLSPKVKVNSDLRASVTSRDLNGNTALHFAAKNGNIHLVQALLYKDIQIDTQNEDKMTPLLLSAYYGKFETLQILVNLGANINHQDIHGNTSLHYACKYNFKSIVEFLLQKKHLILLQNHEQKYPNFYIEDTDIAQIFADYLEQSNQKRTKEVIILNTQTETILKMFQRRNIKQIKNIIQTSKSQNNFDFTQESTPIIKQLQTKLQQLEQFKTQLQAFKKQTTFSNKYSESCKNLKENEEIQLSQFQILGLIGKGSFGQVYLIKRQNQLYAMKVLDKIMILKHNLFRYAQTERNVLSVTSHPFIVKLRYAYQTSDKLIMILDYCPGGDMGQLLEDVKRLPEEQVKNYICEIILALEDLHQRDIIYRDLKPENIVIDADGHAMLTDFGLSKEGIFDANQGARSFCGSVAYLAPEMLKRQGHGKAVDWYLLGVVMYELLVGVPPYYAHEKEQLFHNIENAVLKIPSYISPEGRNLIKSLLQRNPIKRLGSGVSDSKEIKQHPYFQDVDWEKVYNKELQFTIPTRKIKINTQVGQNLFENEQVELSSHIQGWSYVHTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHX0</accession>
    <name>A0A8S1NHX0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0810198</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NHX0"/>
    <dbReference type="OMA" id="RYHVEYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH 2/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065:SF3">
        <property type="entry name" value="FI20425P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="12">
        <location>
            <begin position="137"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="99"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8088595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="352" mass="41073" checksum="5AFFCE29205CFA69" modified="2022-10-12" version="1">METETSPKILSFRINSNVNTLNSDLIRTNQCHLDQSTSKINEQNKKIQQLVEGVYSENTDRNNQDSTNNTSCISNLSPLKPKQGQKEFENQFTFVSQEDSYNQSPIQKQSKQVKTSRLSISNKINTRKQSSSSSIKEQIQEEKYCGICGQADQFSNFIRPCLCKSKQHIHQQCEQKEFCEKYIDNQFNKVTKPLQCEKCHFNFVIKSYKDINFTNAFKDPLKHRKVLMYLSINLSMFILITTLIIISILNRMQFRYHVEYIAGLILACLIMIGIIIVVTYNLIEYISNFTWYILDREQAKVNDNGIRMNQQTLNVALENHQKKKQQLLQMMDQMISKKVHSELPIQNPENIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NK51</accession>
    <name>A0A8S1NK51_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0840138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="379" mass="45153" checksum="B35F0D269ACBF941" modified="2022-10-12" version="1">MSDQEETTPRGAKTHKGXIDVLSRQGSNINLQELNDLFHQQMAFDCLSTEKINYQDFQLKDVTFKKKVEDIHDPRRCILFRHFIELIVRLSYLKYGNLIDLHRAIERVIISKLTPYFERKKGKQNQSLDDTKKSTHPKIIIGDFIDEVKPIFDVWSIENRETQFGFTDRTINLEQIYKLLNASEQDKLILISYVDKNFDTDEGQITQQSISQIDQSSVNQTVREDGNKRRKKQQDQTAEKDQRKTLEKSTSIIKYELLLFEFTDILTSFLVKKHKDLHTNKEDEFKIRMSNHIKKLKNRKPPTLNKYKQPRLEPQTQKDIQKEQLKQLRIQQEIKARQRKLLEEERKQEERERNLMALEDNDIKSEHEDDEVESEESDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NLK3</accession>
    <name>A0A8S1NLK3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0850230</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NLK3"/>
    <dbReference type="OMA" id="CAGSIYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015227">
        <property type="term" value="F:O-acyl-L-carnitine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902603">
        <property type="term" value="P:carnitine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006839">
        <property type="term" value="P:mitochondrial transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF4">
        <property type="entry name" value="CONGESTED-LIKE TRACHEA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="2"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="98"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="197"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8090363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="32399" checksum="AC5C2AC22B89D82B" modified="2022-10-12" version="1">MKYFIINFVSGTLSSCIHTLIGYPLDFIKTRIQIQHKKQGMIKAGIQIIRDEGVKTLYRGVSIQLLNSICAGSIYFPTYEHMRKWFARMDNLSEHSYLPFQQTFLAGSMAGIASNIFACPLEYGKILSQKTKLQKMEQTDGPIHKLFQIFKTQGFFNIYKGLKLQLMRDSIGCGLYFVSHAKTLQYFTPQGRDRTEASQFGILMASMSAAASFWSISYPIDIVKTRYQVLNESTIRIIKQIYSEGGALAFYKGFQVTVIRSVIVNIFSLCTYENLRRVGFKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NRT9</accession>
    <name>A0A8S1NRT9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0960101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NRT9"/>
    <dbReference type="OMA" id="IHTFKQP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="422" mass="50040" checksum="4B00F39F0FF79EE5" modified="2022-10-12" version="1">MMRLNITRLTKRMNMKPPSENDFSTLLIAKNTPIRQLSISTRSSKHSQFIRRLPTNQNQQSQQLTSRTISVHDAHFHSEPLKEKQISINTMQNKKKSTRRLIGLQTQPSLIIKPKFTDIKYEIIDIYQLKIAEPKILYKGNRDTNHLQRIAIIGQSVSLNKQLIQKIKSECILVLLTNNLSQIDEQYDCIIQPNHISPNDVLSYQRNINQIWINVNHLIHTFKQPEQIIMIDEVFNENNILNIEDLKNKILYQGNCKMIHLFLQKDKPNLQKLYYALCHLFKYEQQDLTQLNLKQILKECSDNCLQIQAKLTYLQKAIDIKNKLKQQFKKEQICIDSPENRAENLRDCVIEYIKSRSTNKITGMYYIVGDQIMRNIQFFRNRQEDKYFKEKRHQETLQILQNKLQPIQHALTCQQQYAFYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NT32</accession>
    <name>A0A8S1NT32_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0990157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NT32"/>
    <dbReference type="OMA" id="YIDANIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="368"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="441" mass="52567" checksum="0A3848C42A3ED4EC" modified="2022-10-12" version="1">MLSPTVTENEHQRKIGEKLITIIRTILITEREVEELKITLAENIRFDISDAFRAIDSNMKGYITSQDIFRFMCKNGFSVHQKFCDFWIFTWSKGDDKLRYQLFLEFIVPKYDRDAAMQCIVRKLYSETGKYKLSFYLEEFVAKLIYKEIDYLRTIEIDKMNLAQLVEWKFTTVFKYVAGNLPVIDKRCLDQLLNKFEIQSLTQQEYNMFLKRFNRNEDMILQKDEFSDAIFPSKELIKEIQPPRDGFQEYVLNHDQQLDQLLGKDQINDGEDFIFKPEEHKIKLTSTQKDYNIQQEFISKKQQQNETIQNQEIYGPPKVQPTQNKALLMSFKPVQIQQPQQQQQFYQNSIDQSIKASTPIQMKSKHYQSPNEQPSQNQQQDLLQQFERKYNASQMAFSQDNLSGYNNYYYNQNSNLEQSQHVQYNQYVNQPTNAQYILSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUB9</accession>
    <name>A0A8S1NUB9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0860123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NUB9"/>
    <dbReference type="OMA" id="SACQIFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035942016" description="Lipoprotein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="9881" checksum="15A00ADA24106EFF" modified="2022-10-12" version="1">MKTIFIALILTTFLVSACQIFQQQQLNQERYFKDQEIATNYQCSNYVRLNNLVESRIHQKTYTTSPKNQELITLFYKLLTIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P0Z8</accession>
    <name>A0A8S1P0Z8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1000164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P0Z8"/>
    <dbReference type="OMA" id="YSESKMY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="202" mass="23574" checksum="0E3F1F8B2DA1ED5C" modified="2022-10-12" version="1">MRAGLLKNYGPTQIFPEGKQERAQSLYDITNHYLKAQVSKNDQKSKSVNTYGVEQRDFYDQLRLKGKEKKNQNQNYHKPIFKLKGDNIVVIKSKDRELYPADIQWNNGQIGRIKGEISGKIDKNYAKIRRYLEGSSSVIDYSESKMYDKFNSYTNITNQERFSKDCDIMFRTSNNAQPGNVQSVGGIQRTKYELDWIQRGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1K1</accession>
    <name>A0A8S1P1K1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1020008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1K1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="254"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="299"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="513" mass="59987" checksum="FC15611B04D1B96B" modified="2022-10-12" version="1">MIENEKEFRCSQGHNQPVVTIAFDPQLKMDQRLLCVECVENLQIDGKIYGLKKIISQMEENQIKKFENVEKIIMIQIQQIESLQSVVDQIKQRVMQQLNELINIMMGWIKNLQQQGQQQYKYSFFKELELMILSQTKVDSTSQKLINEIQKINISSTQKLYSKLEYFKQFEEYNKCKELLSCFDIDWQLLKQIKNKLLSQSPQKNKNDEYILPYNDSCYAIAINKDCSELVAGSDSKINVFEYKQGLLTSIQTLQEHQNRVNILKFMKKSNQFISGSVDQFIIIWQKVQNNLWSLKNKLIGHSSIIYCLVLNNDEDLIISGSNDKSIRFWTKKNQWLCNQVITDHTNSVFGLSLNQQQNILVSCGDDGQLLIIEQSIQNKEWIVKQKIKVEQYGYRVCFIDNNMFIFSPFNSEKLFVYGSNQYFKKMKDIPVMCKTDGCCLFPQQYIDSKSILVSKNGEYVNFIKKNQNGDFITYNSIHFGTCTLFGTISDDGQLLITWDIKSKQIKIRVIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P3V8</accession>
    <name>A0A8S1P3V8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1050013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P3V8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="380"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="422"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="464"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="506"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="548"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="590"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="632"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="672"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8097742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="796" mass="90495" checksum="AAD33AAD4C0A137F" modified="2022-10-12" version="1">MICLAPHRCQCQRKLCAECQDEHNVDLKYIVSIPRFKKKVTNKLNLYTLNENSEIIEKMIKYQKLLNRSQAKLIHIFENLSNYVTSVYKMIEQKNLSFVNLINEDTNLAESSYNDLEKLVQIAEGTTLEDWNEGKISLLKKMDRMMHWFNQEINSLCSKEEMNEIYQIINSINQTEQVVERKKDFYEVLIPFNNIDIQIFKSIFEILSEAKISDFLGFLDNQQHLEQYVDRQRDLNFVRSNILKITNIIRDIKEHDFNKYDYSTAKYQETKQDLIEKISQDKMIIEILQFLVRLTAIDKSFIQCGSNALNLLVDMKVDLQKQSFENIRIKNTSLIGANFVRCNFSGSEFNNVDLSGINLNGTQLFNCKWNKIRIHELNNFDGHSNSVHSVCFSPDGNTLASASEDYSIRLWDIKTGKQKVKMDGHSNLVRSVCFSPDGTTLASGSDDCSIRLWDVKTGQQKFKLNVHTSSVLSVQFTSDGTTLVSSSADKSIRLLDVKTGQEKFKLDGHIHYVWSVCLSPDGANIASGSYDKSIRLWDAKTRQQKAKLDGHSEWVSSVCYSPDSTTLASGSGDKSIRLWDAKTGQQKAKLDGHSEAVRSVCFSPGGTTLASSSDDKSILLWDIKSGQQKAKLDGHSETVRSVCFSPDGTTLASSSDDKSIRLWDVKTQQEKLDGHPNKFLSVSFSPDYSILASSYDDNSTRLWDVKTGKEIQLSDMSYQECLVQFPKHLFQNNPLSKVNSNITLLIISSKPIFQAQGAFIFKGEFADQLGIDLRTFFKQSGGCLLENQIQLEQEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6H6</accession>
    <name>A0A8S1P6H6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tRNA-dihydrouridine synthase</fullName>
            <ecNumber evidence="8">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1070120</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the dus family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6H6"/>
    <dbReference type="OMA" id="ISPPVWQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF5">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(16_17) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 8" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="9">
        <location>
            <begin position="22"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8098596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="363" mass="41877" checksum="C1FB3A59D74924EE" modified="2022-10-12" version="1">MDDHIQQAWQFYRKLGSPKFICAPMVDQSELAFRMLTRKYGATLAYTPMLHSRIMTESKSYKDEFFTTCPEDRPLFAQLCGHDPQILIKAALIIQDQCDAIDINLGCPQGIARKGLYGAYLLEKREQVLTIVKELKQNINVPVTCKIRVFKDRKKTLDLTKDIQQAGCSILTVHGRTKEQNKDFVGQCDWTIIAEIKQQLQIPVFANGGIYTWSDVERCLQETKVDAVMSSEALLENPALFSGEIKDLNDLAEEYMQFAKQYNARLSEIKGHLFKLLYTGLQIFTDLRSKLGSAKTYDEHLEVVIELKNKRANVPKEDKLGWYKRYQNFKQPQNNKDKNIQKLENIIQQQDLLLQNNDPKQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P946</accession>
    <name>A0A8S1P946_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1090078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P946"/>
    <dbReference type="OMA" id="ETNQQMR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="221"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="354" mass="41389" checksum="8FADA446BD4D15F5" modified="2022-10-12" version="1">MSTSSDFQPPSPSLSPSSQIKCYQEQLEKAHSMIDYLEVMLAEKKALKENFNSFNIPKTKSHMKKQSLCLDNIPQSIQIQQFVTPRETNQQMRFSSANMPSYISELQQKHQEILSSTKEKSTVKKLQIQEIQEKVQDLQDEILICKERQKGTELARGQAEEVLGQIDDEILLAKQKLKQVKSQGEQIYDSMMKLEDQEHDKKYYMGRTSTLKRKRSEIQPPNQIKNQLNQLKNEIDQLKQSKKEKEEFYSQKIREIEHQLDQRKTNESVHDDQLNASLSPDRQKVRVYRGKQALLQLTLNSDSCSRSYALRKSQENKLNSAFDFKQFDEHENVSIDSFKNKDKKNRTCDPCTIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P9L2</accession>
    <name>A0A8S1P9L2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1100044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P9L2"/>
    <dbReference type="OMA" id="NIQMIMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8099594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="138" mass="15647" checksum="90E95C701F011E51" modified="2022-10-12" version="1">MGNICKSQKDTEFEEAQQLSLVTIETGIQSINSTIQANSQQLKLNKESPQDGVQISNIQVIMIKPRNEEEGIQIMRDLIQFGNKIIIIPKATDESPNVSAKSLFKKGILKNKGTPITKFQPHSCKQQKMDKYLRFEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBT7</accession>
    <name>A0A8S1PBT7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1120116</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PBT7"/>
    <dbReference type="OMA" id="HDKEMKG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="79"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8100439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="146" mass="16883" checksum="D4ADAE06A031A218" modified="2022-10-12" version="1">MNTQLKQIYSAEKVQRFELLFLFHDKEMKGFISSKDLPFILRAAGLLISDKEINDLQVKEKISKDDLFKLLIQLKDKRPVKQEIEASVKVFDEHNTGFIDTKELMGIVQQIGDEQVEKDFQLILNSVKGKENQVSIDSLVNLLMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PC30</accession>
    <name>A0A8S1PC30_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0750092</name>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1120097</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PC30"/>
    <dbReference type="OMA" id="CASILCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.100:FF:000001">
        <property type="entry name" value="40S ribosomal protein S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_eS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_eS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_eS27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11594">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01168">
        <property type="entry name" value="RIBOSOMAL_S27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8085832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8100401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="81" mass="9203" checksum="69997FA612814FAC" modified="2022-10-12" version="1">MDQDLLRPQYESEKRKHKLKRLIQAPNSYFMDVKCPQCSTNNTVFSHARGIVTCIKCSSQLGRSTGGKLQLVLGAKYKTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCY2</accession>
    <name>A0A8S1PCY2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase-like domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T1130130</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a fundamental role in microtubule organizing center structure and function. Component of the infraciliary lattice (ICL) and the ciliary basal bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the centrin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="WNSEYED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051581">
        <property type="entry name" value="Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524">
        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524:SF6">
        <property type="entry name" value="CALCYPHOSINE LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="883"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="919"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="1085"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="1121"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="1375"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="1407"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="1867"/>
            <end position="1902"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="2089"/>
            <end position="2124"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="2139"/>
            <end position="2174"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="2175"/>
            <end position="2210"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="2253"/>
            <end position="2288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="956"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1529"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1647"/>
            <end position="1675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1694"/>
            <end position="1723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="958"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1547"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1651"/>
            <end position="1675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1709"/>
            <end position="1721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8100849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2746" mass="323445" checksum="3E1DD1B87101B8DF" modified="2022-10-12" version="1">MSQLKKFQQTDFQLIYAINNKTSLQKKGAEQFICKLVIPQKKTTKELVDEMDQMKIRLSKAGLYIPEGYWEEKQALCFIYKKFKFSMADLIKQHKRQGLYIPMNKMQNYVNEFTNWVDKIHKSKKSDNQRYFHGRIKPSNLFEDEENECVITDFFIFSNENLELYLPPESLAALKKNQSQRFVSGSSVVDLLGGDPIKIDIWQLGLSFLQMASLKDIEELIIFRDSNTQDSAKAIEIKETIKKLYGQHFYNLIERMLERNPSTRSPIHELEDIIQDFFSDNVIQHDENLLQPNPELLLDELKQRVTQQKQQTVVQYLSKYQLQPVSRKIKATNFWDALEDMKISVSFKEKAVLLQMYDIQKTDEIDLTQWIQRQKEVVKLGELTQTQDQELFDMLKDINTYLEESNINLIEILKASDQEQLGQLPFDKFDSIIRQEGINLNPEDIPLLKLKYDPNKKNVIYYESFCDDIKQTNQQQLGIDELKKKMYKAIRDIYNVFISFFRHYDSKSKGYFGYDDIYKFLNEIKHQPTPEILKQLFINLDPENKKMIQFENVRMFFEINVNEDLKDIMTQLVNGLLSVQMTLPQLIRSSTKSSTIISQSQFYNTLKQTNDHLRQTDFQFLLNCFGVKDEIEYPRFIQAIVQKARELQVQNWKQLQEADGGDSIQERKSNFDDPELSLQKSINFNPKSFDSQKKLLQFISKELQQRDETARQYFKCTYQRMSIQQFKDKLRQLLVHKADFEVEQEELINNLLVPNDRTYVDIDLLIEAILYYKDKRNDQIRYSQHNAVIFFEQLNKMMKKKKITYSDIEDLDSLGDGSLKKKHLQDYLIKQFLIDQDDENFKDFLICIQQDQQNISLIKLRQGLKLDEAANKLLQDLNTELAYEQSKPEQLFRKYDANRNTKLEMREFQEFLQQLIGDVDQKIIETLFHKFDTNNDNTINLNEFRQKIINKNDENPRQNQQQYQSKQQQQYQEPQQQFRRTGAVDSKVLTPQIMKSVFRLKDALNENNQTLQIFDKRGRGEILINAFQDIINQFGFDYVEFRSLAQYLQSPDDQKYLQYDKLQILIDDCLEAEQLLLSLNQTIQAGKNATAQLFYNYDQNGNDVLDRQEFNKLIHDIDPSIKNKQIDNLFLLLDANGDGSISISEFKSKVCAKEKPKVLSKDNQPQQGGMFNEVIKFIKQSTTLLSDFEGMDENYTNKLDVESILSVFKKNNGPSLQKLEMKGIADQLGSFQDNQINYKMLCSKAKQHHIDTPQNKIDAILTKLREQLQQKYISVIDIAQEFDLNKKGKVKLKGLKLGIADKSIQILDNDWDLLLTILEQDAQDYIDYYELNNLINKGLTNYKESKKISIINTNQEIDKIVVSFSQYMEKELCTLLHMFRQTDRDKDYYISNEEFTDLLQRTIGYSSTQDVQRQLFEIFDMDKDGKINFTEFEYQVYKRNELTKQQIEDMKLVMLNGRNPQFEQQISELFYIISQGSQSIDFRQFITYMNYYKKYKIIELDQFFRYFDKEQTEKLDQFRFILSFKQQQGRSTPQYEQPPQQQPYPPGHYQQQSNQYSQPNPYQQPNQQPNQYQPNQYQQQQNQYQQPNQYQQQNQYQQPNQFQQQNQYQQSNQYQQSYGNQGYQQQPYNNQQQSMQFKNQNEIANPMLGSFNPSNISQSIVYPPQNTNYNSQNNQGDMDVKQQWVQNNNKIDQRWSQGQDQQMLPPKYGPDKWKENNDAQRDNQQSIIPQEYDLSFYDQEIHRKCIQKNIDLFDLLCQYDETALPELKISRPEQLEQAFLFLEIMAPTPHIQQFYYYYSQGQNQMSIVLPYLKMNNPGKLLAKALNFTMVKHKQYTKQQLWNLIGEQQPTWQMPQLDKINRNLKIGLNERELREAFQYWDTQQSGIITYKMYDEILNLNQIIVPTKNNNGQQQQSQLMEQGLKQTLDEFLLHLSEVVLSKNCFNLFEQYDKKGYNQIPYNDFLQVSKKLLGTISGEEMKAIKQLLQLNGFINLSDLAQSLKVDRNKVGQFNQDDEKQQFGLANKDQRKNTINSQNKEKFTKMLPIINEFLKKRNKTYDDFALYFFPPNQVSTIDKNIFVKKALEAQFGLTAIQCEDLYDYLDANSSGHISINEFRLVFQSKQGDLQNKNKAEQIIMSQDIEQEIQELFNQIDENKNQSLDQRELLKALQSVGLNPGTEELTQYFAQFDRDKSGTISYQEFSHIVKDILKKELLQADDLLEDLRREFRQVCNPTTRMLSKEQVSQVFQNMGVQIKNEELEDLFIEIDEDKSGSIDIDEFIYFIQKNQSGMSAKASAAVMNIKGSRRISLHDLKEIFLTLPQNFIMSFIRSQNKKLQNLPSQQLKPILDNCGFFYQGLNYVDAANFNIKQSILDKVNIKNNFIAEIRLIEATGIPIPDEKDVPRNSFLKREIGIILMDKALNKFDGNAIYIPAQWNSEYEDRWIFDSAAMEQAMYLRWGNFDEKMDNMTELVFEFITYSTNKGRLIQISCAYGSIPVYQLKPGKQLLELKGGAPLKDITIDKKDIRTNRNGWRSVIKALSSNIKSQLTIEVVKLTPQTIYKLSALPYKCLLNKWALGMQSAFREYFAYRTQMKGEIELNLASDIRIKAWLNCFDCPDTIRAVAQFWNEFIEPKVNDHQFLLKAVSKMADSLYLMFKNTEFKFSAGDPTGRIDHDMVLLKKRQQLIADAILDMKTNLGIGNQQQKKQQTVISLEPLNIEEMMDLENDQDLQAIERFLNKKKPKKQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PEQ6</accession>
    <name>A0A8S1PEQ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1150107</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PEQ6"/>
    <dbReference type="OMA" id="ENKWVEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051746">
        <property type="entry name" value="Kelch_domain_containing_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260:SF3">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="94"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8101429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="66156" checksum="AB08FA4C48A618C2" modified="2022-10-12" version="1">MSQQFTYYSNELCQIHQEPCTNMCINTKCLQPLCPECIEIHFREHKECQIESLKSCRTNCSKKIRSSISEMNQILSLQEMKTIENSSQYIEMCRDSLQQLKKQLIDKIELYNKKLLQDFNNHLIQTFKDSQIKEVYNRIKNLIQQLDELGTKLETQSDCQIKTIRSIYLLNTTQLIQSASQEAYKEIMKLKFPLLVVSQNLNQKLDELFVQSIQIQFKKTNPSILKIEDKLQQNIQPINVQDIASMNQSPLLSQINEIEQAKKQSEFTITMEDYLLPNVQNKVLHYFKYNSYELNLINLDNPKITKKINLKISFPIPVFHQSLITKTGQLYVIGGTTIDNNYPNNKSNGIYLFDKNSLVNVGHLLISRSSHGCCIIGDYIYSISGLGQGQTLEKSCERFNYILRKSEKIQDCLYPSIASCCVNYNNQYIYKFGGLYEKQQMNFIERFNIKDMIWQAIDPQITNKDIPLFKGLKQFAAGVQISSNSLMIFGGYDENTVSSNQSFLFTIKNENHILHMVNVKPLKIPGGFWHSQCIIYKQELFVVQNQENKDSETLDTKTILRFKDKWTIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHR1</accession>
    <name>A0A8S1PHR1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(P)(+)--arginine ADP-ribosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1180188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHR1"/>
    <dbReference type="OMA" id="INIMASQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="182"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="59475" checksum="6480F238B4AF80AE" modified="2022-10-12" version="1">MDQFYQKNQEYPIIIWTDSQISNWENKLYCEKLQTDYQNAFQAFNDISEAIKILQKIGDKDVTILTSGQYAYQLVKHTKKNIIVFCGRRQNHLDLFMNNFQIQSIVSDSFQSAHENVIMSMNYSDIQNQLIKLLSPPQQQSMEYQHSKYHVSIQFLVAIEEIKEEKRPNQQDVFGEIENLLIENKQQKMSNILKDLDKQEQKKKKKRTKTQICLRNLFIFIAEMRFMKSLTNCLLSMIINQQKTLLHDNQSKEDKFQNLFRGTSFDQQGIFQQIIKDLNQSRDEGASIFWNTIASASQVRDVAKMYAKEKEYGILYEITLDKDAPHPFFQLQDYHSHYPVEKEVILFPQFEFIVQEITFTQEGSKQIYTVKIKQVKNNYAFALDPTKRKIYWDSIIEQKIKPKIDTLVNFQSKRIFELISFSNQNYNKEIQRKIFSSIETELENTFQLIHNQLQQIFFHSKYPNIVNQITSLTKTYTEIVKCRYDDQSAEIAKLHIDQVMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJ00</accession>
    <name>A0A8S1PJ00_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1190038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJ00"/>
    <dbReference type="OMA" id="MEYEEQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00195">
        <property type="entry name" value="UBCc_UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="18"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="152" mass="17991" checksum="E04C9BEF93037660" modified="2022-10-12" version="1">MFKFSDKRMEYEEQMLEENQDELPPNIQYKRIDRYNYVLQIRGEKGSVHEDAYIIADVSLQGFPVEAPSINFRNNFQHVNIFPMGKLCLYLINKETWISSTSLIDVFSGVNQVIHHPTFNDPANSSFKEPDVYEKDMKEQAKKFRDEKYKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJ44</accession>
    <name>A0A8S1PJ44_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1200063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJ44"/>
    <dbReference type="OMA" id="AKSSYWT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="33120" checksum="A6546701D0700308" modified="2022-10-12" version="1">MTELAKSSYWTDLEQKITNETVLQTMRKFGQFEHKINDVIQQIIDSHELTYKSDVVMDNGSVYTGEMRNNQRCGRGTQIWKDGSIYEGTWLDGSAFGFGRLIHADGDLYQGDWNGDKSSGYGEYYHLDGAVYKGEWSEDKQDGRGIEKWPDGAIYDGEYKQGKKDGKGKFAWTDGSKYEGDFSQNDIHGYGVYKWADGRVYEGEWKHNKMDGYGKFVWSDGRLYTGNYKEDKKQGFGKFQWPDGRSYEGQWENGKQHGEGVYITANGARKRGEWIEGKRTKWLETEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PM75</accession>
    <name>A0A8S1PM75_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1220166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8104051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PM75"/>
    <dbReference type="OMA" id="NICDLHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="924" mass="108979" checksum="711F8FE47C0E3F4D" modified="2022-10-12" version="1">MQGKYNFIFSLDGEQKSFFVSNLDVASFCRLIRSAFQVDKTITALQDAQGNICDLHYFCNNLHAHKDKFYIILSEQRKSERQSRKSHKSKNSRVLATNEEPSYYVDFGAFLQEIQQIRFSPQHPVLTIVLVFDNEYQAGIFGQPIVMRIYKTFAFLQAYYTIIQEEESDSLLLTVFENQILKHEMEMKINHSIAEDVCYQIEQLLCEEEEPEPILQGSFATPNSNANNNIPLIVTRGNEKQILQVEANDNFHSSQGKRTHGVMRVKKAVDKFRNRFESREYEYLLGLIIGQDQSVFAAYQLFQKQQDDENFIRNLKSLLPQEDNNNLSFQNNLKFITSPSRSDLHQSVNFPYDCNPTPGLRSSVTIMDQLLNSGDLDSIESGMLRELRMDSDLDDLVKSLMDYPQDVLLQIIRQYLQSQFQQEITKNFNSHQVEYFLQENANKQNPIYEGFQRFGQNGQLQDLFLFLKDNVGQLEQQLREESSYSYYLMHKRRKDSHDEHFHFHLNLPQMNQQEERKLSVDSILLESKKEINFIDKYHVKVLQKYSNEDYEKLFMTVYDQCSKFCDEHEKAKLHGFYTAQNQRLLEILDKYGQRYDIDSIKSDINDLLTLQNKKIDEIPSPTHGAVIEPKMHKYQNFKFIINFLYEIDKVITLRQAKAFYYLYSIEDMSVMAAYEVYCETKEQDEFLNTLNLIFKVYASSSRFDFNEIEQFDSLIEQQLTILFAYRRCLNQEQRLALEQNMISCDNTLLKLFRDFLRQRDQRIFIQKLSDFANSQIEKQKDNQSMDPYMKKIDQNNQHKDLIKDICAKFLKSKVKQIDLLMDAIDKDNIMLKSSLEVYDYNLDKQDLVENIQLIYNYILKSNVKSILKANLIELGRSKTDLTYLLRNINQDEPILKGAFELYFQTKDEAELKDTISRILKFSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PW90</accession>
    <name>A0A8S1PW90_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1340039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PW90"/>
    <dbReference type="OMA" id="QKHYGTH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="29"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="155" mass="18311" checksum="ABF844FBB6BD3B33" modified="2022-10-12" version="1">MISDISLNQEQNLNINSPPILSFLKKQRKRSIQYVTFKMQTVKIHKTFAEYQVQVNEQGKIWIMEIRYSTLWQFYQKLKKLFGIKLTFPSKHLFGNLSPDTLKKRAIGIQQFLQELSSNYKLINSDECSQFLTKQKHYGTQVIDLTEIEEFVLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PW99</accession>
    <name>A0A8S1PW99_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1330121</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PW99"/>
    <dbReference type="OMA" id="THLINRN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF7">
        <property type="entry name" value="NOSTRIN, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCH" evidence="4">
        <location>
            <begin position="27"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8107344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="546" mass="64386" checksum="6FE3B46B9BF012BF" modified="2022-10-12" version="1">MNQTQKLAYYPNLLGQSERIISQNIESKRGLEEYLAILQERANIEDQYAKSLHKLSQQIDRSSITYLREQLVNQLRDKIERLENFVELIRTEIVEQVKEILQDQNQISKKIIDEMKGLEWDLQDKQDDLMGGKLDYKLQARELEQSGISDMIYQYSLDISEDKAIKQASKTQQIQSECIKLEREFEIIVLSYNEFIDVYKTKMESYLSQMEQQEQQKLLVQKDTLMKLFLFESSISKQSLQDTEKLNDQIRKTNLQDVIETFIKKYQRPQEAPRQLEFRPYSSFLGKTIDSMKSQEYQKLKDIIKAHNDTFYNIYEDCVKKNNLQTIEEQELTHLINRNSVQAIYKIMQIHINCVWNANEIQNNETIIGLLRQSTLNRLLWCQALEIQGNKSNYKVPSNDAYEQIVKWSQKMINLCLEFQDASPLRKLITVSSIINVAVQNKKHFLMYNIKENKVLQTADFIEASVVQAIYDALVEEIKEVTTLEERIRRQKINILTQLSKICFTLLQFNPISVIKMYAENYLKQLSVANDATENLLFQIESFEQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXG9</accession>
    <name>A0A8S1PXG9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1350098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXG9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="89" mass="10818" checksum="31DFA60D972144D8" modified="2022-10-12" version="1">MINDKLFKLIFIETKRFKQFIISNRIIKSFQCYSNLYLIAEIYYNEFSRLIINYILSIKIQESSKVEIKIKLNYKPTETEMRESGHQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXQ9</accession>
    <name>A0A8S1PXQ9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1350052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXQ9"/>
    <dbReference type="OMA" id="SIHEFPP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="609" mass="72625" checksum="FE214DEA6817D68C" modified="2022-10-12" version="1">MQQDNQYENTYYQPKGFIMHTRQSIKPFLLPQTEASTHRFSQLDYFTEPTASIVDPQGPQTDRTYVRNNRQLVKHIKPVNIEPESNILNEEKNKLQTKFNLLDSFTIRHSQHHLQVKNDIKQEILQRKFSEQKQGFKVQQLDEEYESHVILRRNQFFYTSLRIKDRYSPLILQIRIKQGDPNTRLRIFASPKFIQPNRFNAAVEVSGRQLKFSSNSEIFQEETLYVAILALNELSIGINIEFGRPTTKRVIQRERKEKSIHEFPPDLELQIEEIMMKRRLRYANQQNYVELNKCSQMSRRIPEKPQTPKYIEVKQKIRRMKVEQSLKRVAQLQVHDIAKQIHRKQLEMQREIKHREYVQKTWFKIICLINILAPGINEFIENQRQLVAKNMKKQISLIQLLNKIRKKVEKFGPNCKLRTILQGKCCLQLIANNSRMIAKRRAQKIITQMLQNNITCFITESKGTHVVNLIKKCVNRLVKHKISRIEFMSNLKLYLSVQYKQLYQIALRYSKQSESAKNKRDPIHFLLYDASFLHQMTKKLLSHVFEKWNKNFITFRNATKEEREKQGKKGVALFWQPPKLFELPQDIVVRLFLFEELEKRGLITGEYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZ79</accession>
    <name>A0A8S1PZ79_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1350026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZ79"/>
    <dbReference type="OMA" id="ARMYPFS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000220">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000249">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="64"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="214"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="527" mass="62173" checksum="7719439C6BF05239" modified="2022-10-12" version="1">MSEQSSDDSDESPQLIQQNRQRKHLSVIDILIATLQIEPKQRTWQQLKFLCDTLEQNVHYFSQLRNKLTTSILIKLMATISLEQFTTFNVVFNQGETGRKMYIILKGEVAVLIKTDDQNEIIQKEDHKRRRATKTFDELILHRFSNYRIVAYKKQYDYFGEIAIEQRIPRTATVIAKEPCICAIITFDAYQSLLSELQADHLQLRQVVIARMYPFSILNEQQFQQILHNYEELNIQGGCLLYREQQIADALYLVIKGEVLVQIKELVDNAAAQIQEQKYFVNFQKQQRIKNIGLFGTGQIVGDYELYLNKKYKHKLIRKTISIVKSDSRIIRIPIKQFVDVIEHGLSAKWLLRYLNEKYEQKEQLPILQLKDEQENRKVQSFISPQFKKMILRLKKHHDHNKISSDRYQQVNYKQSLEDGNTEQQFIGLKKEINYQDKSLLKYLAKDQILHKRKSNTLTNFSIEKFASTLKSRVKFTKLLTQIDEETSCYRFHSTPTKTNGFSSKQIQTLTNIQSPQYKFNQTFKMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2J5</accession>
    <name>A0A8S1Q2J5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1410112</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2J5"/>
    <dbReference type="OMA" id="FIVTEMC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="9"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="591"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8109688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="652" mass="77375" checksum="548A91AC574694DE" modified="2022-10-12" version="1">MKQIGHYTYDEINCLGQGAYGKVYEGLNTLNNETVAIKKLDLILFEKDKYLRKQIVQEIEIMSKLNHKNIVKFVELLATKKSLFIVTEMCRSGDLKSLIASKNISEQQAIDIMLQILEGFKELIKSGVIHRDMKPANVLNDMGTVKIADFGFAKYVENYSSQLLKSCVGSPLYMAPQVLERHHYSTKCDIWSIGVIFYEMLHFDVPWKGRDEEDLLHNIKTQPLQFKNDLSTFSKEFLTITLVIEEENRASWNQLFDLSNTSVLQKSVSAEDINEESIQKLPTWVQRPSFQQRQDKKVEEQRYQQIEELQQLRKQLAYQHFIMAECYNEQINNEKNGELQQESIKQLINYLNDNIVKQSQDLIQTSLDLYSKFSQKNQLWKMQEQLNLEHNYYVQMQIDAKKLFDTNQLQGKPDDRLINEARNQLQKCKNSNIGCFNIVRFDKQLKLENMILIQIIENQNCQIYPTVQFQFFFIYRYNCYHNQFFIFQLFFHLINLIKIDSKIIRYQISIKIQMASANQQFSFFQDYALKKIQFVVDNIRYEYDNHRMLWSTDVIEEDDNPDEEINKEQLKKRGLKQIELREQVRQNEHTISKLASEIQLLNDNINKYQEKLQQSQAEQNQLIDEYVHEHLETEKIKAARERLDKLKRELGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q704</accession>
    <name>A0A8S1Q704_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1470013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q704"/>
    <dbReference type="OMA" id="ITKKRFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="625" mass="73307" checksum="AE4EC26BB1DD09F4" modified="2022-10-12" version="1">MLEEYSLLLKSQNTSTSFQRLLQEKMGSQEVLITTICQFSKLPVIIPVRHSPINYIHCVFELEYWLEHYQLMYKKVDPTGFACPGCRKFANFKDYGIDYTFYHILQELELFQKNNPDLKLNTSQLIYKQSNKSYYVEQKITKKRFQLPGSNPILGVTRRQQGLNQELFQTESAFIADEISQTLVKLQSLIYNRIYKILKLPDYSQYTFNDLQQKIALKKSDLKQVLIKSMKLISLGRQSLQKDYVFALKKITTNNQVQSILIVYLVQYGIWYEFPLKFKGQPYVQLEQALYVKGEGSLTHNHIYMIGGRKTERGQQSDQVLQISFPKSIIQSGQEAKITELPKLPKEGYNFMGICYKQQLFVFYGQKQIANFDNAIRYELLNSQYIFRNNHWEQMKLKLVQRFDGSFFTMNHNTFDKLIVFFGGISREPDSFPNHRGPQQHLGQIFSCRDEKFIGDQQQSFKIQFLDDDPYHRSVLAYPVFSCPYYGNNQLLLSGESMKFRNTDQRQVYTFDWGNATLKMNDLFSLDPPEHILTPYKKKTIGYDIFCPVQDSEGAIAYGNFYIIHHCEVEQQFYEKNPKTVTQLLKVNLTNGLFVAIPYIDSKTSKEIADQKYEKLEDKKNQEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9N7</accession>
    <name>A0A8S1Q9N7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1470112</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9N7"/>
    <dbReference type="OMA" id="LIIKAEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052788">
        <property type="entry name" value="RING-type_E3_ligase_ATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798:SF97">
        <property type="entry name" value="ALCOHOL-SENSITIVE RING FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="90"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8111431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="137" mass="16832" checksum="278907D40AF1BDFB" modified="2022-10-12" version="1">MNERRQQQFKDAYERVQEIKKRIHKTQIQQPRFPWQFMFLYNIYMNLNLKQLPQQIPQNLRIVKPRKPKNWFDDIPILVGVDEITQEEICAICLQEINDKDLIKILQCNHYFHNQCIKEWLIIKAECPTCRDKIQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9V6</accession>
    <name>A0A8S1Q9V6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1480015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9V6"/>
    <dbReference type="OMA" id="RQLEQNW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="228" mass="26993" checksum="E256FFB295D747E5" modified="2022-10-12" version="1">MKGPKYVENNFQLLLARRLEYNKHINQVKNAKCNPMLEKRSLSQQQSFFRQLEQNWKQANLKREQMKYEEIVKFNTKMLIKVANAKEDQLLSQNTQKGRIDSARSSKSLHSVIKKTKERELFQENKRLKDKIDNVQSSIAYNKIIENFNKHQGERDRLTRNSYQKQKKIKEMLDSLTSQSKTSLHSKNNNNKNQVPKLRLPKINQQGQSVKSIQQLNKLRVDGFYTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QAU5</accession>
    <name>A0A8S1QAU5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1500017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QAU5"/>
    <dbReference type="OMA" id="LELTFYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="495" mass="58886" checksum="F90BCCF72819DD11" modified="2022-10-12" version="1">MDSFIHTIQSISNILDAQTDLKHIKNTFKCLTSDYKLQLKINSLLLLIHLSQQDKIRNALNKDQLLYDFILQTCQNEQNALNQISLETLKYFGTVAQIEKYRQAIDILMNQGIDLPNQYIHANQLFELQRNLQNREVLTLQLFQQTVYDHQLDIEGIINIVQSISIVEEKHICMLDSYKSTFTERILSIEDLLKNKQNILRIEQLEQLKSIQYFEQQFNKQVDKQQKDKYKNFNQFQEWIAKSLNIKLSIKENQQLKQQNQLPQQLHQEQYQLQQQNNQLQNSIPILVIENQPSDRTAKSVRSNIVQEPQKNPFHEAEKALRHKIQIELEKFKRKQLPDKQYSGQLVKESLNINQMDQNQLKKFKLSSIKTKQKLFDSPEIQLGVIKKFILKEELNCNFLELTFYIGNKRQKILENCSLIFDEPRGLQFWVPQNKMETQIMGRKQCKMSCLIGYQYSLFEPLKGKYNQQEVNFNGLRMIRFVRQFLWFLVYQVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDG9</accession>
    <name>A0A8S1QDG9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein tyrosine phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1510110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000156">
        <property type="protein sequence ID" value="CAD8112490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDG9"/>
    <dbReference type="OMA" id="REQRAYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00047">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="2">
        <location>
            <begin position="189"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8112490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="33764" checksum="B8944D5B441D2ED4" modified="2022-10-12" version="1">MKYEMAINLQEFVKSFNQRRLEKEFSYLCQITETQEHEKLFFWNEKTHHLNRYIDILPCIHTIVKSDVNEQFYINANYIRGIDNIDKQYIATQGPIHSTVNDFWHMIWTNEVGLIIMLCKLYDHNYKQCEKYWPDISNDYGPYEVKLKKSEELKCGLISNQFIIKFQQQEKLIHHYQWNGWPDLGVVNEDNFVLIDFLANQANSSILNNKMTVVHCSAGIGRTGTFLSICQIKQLLNSKEAKLSIFSIVRRLREQRAYMIQTVEQYQMVYSFSLWLIQNLKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKT8</accession>
    <name>A0A8S1QKT8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1700034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000179">
        <property type="protein sequence ID" value="CAD8116239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKT8"/>
    <dbReference type="OMA" id="IINQACQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="462" mass="54380" checksum="E69F559F1F101952" modified="2022-10-12" version="1">MINKAASLPRTTFQMFKMISKNQTRYLYGKLEHFFANYEMSEFESPKIANNKFQYLIEDIFHSNSLQFNTSLASYLNVNDIEISQLIILLEKIYLNNLFLSRSNKQCLTQIVQNQLNSFNEDTISIINQACQLGFINEQLMETFINQFTNQESKQYNFTIQVQLLYIFGQALLKYNNLTKNEIFIRQWQELIKNAISNYKPDYKISGCMFSQISFLIKGLFYIKLEQEATYLGQIIELYKCYYKLLISNFAESLDFNNMSPKESTILLTALLKLKDQFLKQEISLITQSLLNRIQKTGVSAFSDLALQSFVFNLTQLGDYQQLMAEDILQLLQQRVQSQQFSPRIMIVILFEMHLKWSYTVSKEHLLKLISSLNIKEIPDFELGSYFQLLTLLPLDQIQHIISNIESEMIIREQFQKVNQQFIKNEPQSTDRMRRSLQKLINLLQNHGKNTESLERTLKKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQN3</accession>
    <name>A0A8S1QQN3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1890006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000198">
        <property type="protein sequence ID" value="CAD8117125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CKMEDCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="118" mass="13891" checksum="9967990933388833" modified="2022-10-12" version="1" fragment="single">MQIRCTQADHRNQQIIGFCIDSTCPNQRPYCNFCLPRHSEHLEMLTSIQLLFEWIQKGILRIKNVEKNVQEIKVALDSLNLFLPYLNFNVQQFPQLGLSQIDKLIKDLCKMEDCEKXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QV80</accession>
    <name>A0A8S1QV80_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T3530001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000362">
        <property type="protein sequence ID" value="CAD8119658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="408"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8119658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="457" mass="53068" checksum="7A79380C73A1FEB3" modified="2022-10-12" version="1" fragment="single">MNCTYHIRNPITFICIAPAPHKCKCQRKLCAECLYDHGVDVINQTLPINKFHDQVMKKVKELKLDDTSELTQQRMAFKLMLSQTESTMRKIWEELQESIKQIYDLIEKQTKSYVDLINQNTNLIETSNTELEKLVQILEGKTLNVWNAQKKFYLIKLEKAKNLWGQEINSFNEQLKKGMYEILSQQFNPSTTTIKPIQEEAEDYQKKEDFYEMLTYIQDIDESLYQKIIDILRKEKVSDIIGFLSRTNNHQFYESLNYKQGNIREIKKQVKKITNFLRNIQDHKFSKDDYSQETYEKERAYLIKKMKANKGITEFIKFLVRLTSIDVKYIQSGSNGLSLLVEMKVDIRNQSFENIRIKNTSLIGGNFVRCNLSESEFENVEISGLNLNGAQLFNCKWKNLKIHELNKLDGHSGSVLSVQISPDGNILASASGGGDKSIRLWDVKTGQQKAKLDGHSX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QVK5</accession>
    <name>A0A8S1QVK5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T3240001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000333">
        <property type="protein sequence ID" value="CAD8119453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QVK5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8119453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="56" mass="6914" checksum="CA650EB788621A9B" modified="2022-10-12" version="1">MVLKDFNYLWAVLDILSWINNYYFLMLLSFQCGIILLKWWLIKSHWFSNLLRIEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNL8</accession>
    <name>A0A8S1JNL8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleoplasmin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0050016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNL8"/>
    <dbReference type="OMA" id="ANKNQGG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoplasmin-like" evidence="2">
        <location>
            <begin position="2"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="219"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="334" mass="38767" checksum="DB94D4D2AA0A9954" modified="2022-10-12" version="1">MFWGTQISQGKTHKLTAQEADVIHVSNVAISEGENKIQVYAKVQGKEFILANLEKNKLEQASLDLYFRADQQVEFGVKGKGTIHISGYIEPELDNEDGLSDEDQESLDQEEEVSEESPEPVQQAKKKEQSKQQPQQQQTQKQTKEKQDQPQSKPQQGQQQQKQVQQQQPKKADEEWDDDEDDDEGLDDFDENDLDDEDLDDEDEDESEEQKPQQKFVKNNEGQKNKPQQEQNKPQQKGQQQQQQKQQQNQNQQQQKQQNQNQQQQKQQNQNQQQNKGNQQQGQKPAQNQQQGQKPAQNQQQNQGKQQQQNKDNQNKNFNKNANKNQGGSKQKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP86</accession>
    <name>A0A8S1JP86_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060374</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JP86"/>
    <dbReference type="OMA" id="QIQCSYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="60846" checksum="B456B40D150BD259" modified="2022-10-12" version="1">MNYEALLEELGLDGTPQKQISKSPQNNTRASDNTYFTKTPETKQKKIKLILNVNLSSNQVEQLMIYQDDDPKLKVSEFCHLHQLDKNAQSVLIKQINSNTNNENYQSQHKLQQQNYYSKQLQSYSTKESEQPGERLYQLAQKKKEQRENQIKQYQTEKQEQEQQQLQQTPKLSKQTKIYLEQAGYRKNGYMREKGTLKDKQIQQVFLDEQGKEYNFVPQINELSKQMSHYIREKQHYENVFDELYEQARNKMYSQPEYDIPECTFKPSVNQNIKIDEDFLTRMEKKQEEQKEKRELIKMKYENVDLKTRQLLYRPKICRSPYAQTQSEKPIHEKLYLQSKLKSEKLQQMSEQKIMEEKESLNVRLKNELSEDIVFLQRKKILKQLFIEMDSNKDGQIQCSYEEINNLSPNIIQTLWPVLKLLQEQKRTLDFDDFFENVYEYCINLPQIQKDIIFKKSKNNYITMKDQDYTFTPKTNKKSKSMNTVHYPSCKCEICSQIAIIQRQLNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQP8</accession>
    <name>A0A8S1JQP8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080471</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQP8"/>
    <dbReference type="OMA" id="FQYTHYV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="122" mass="14504" checksum="4AA67692CC3D68E7" modified="2022-10-12" version="1">MFSNQSEYDLRVFMSSYSDALHCLKQSADKSTLRKNYENIANRLTIYVKQKKMFGEANLSKNEERDILAFQYTHYVFLKYLPDFDNFKGNYEQLQTILNNSFNSLSSDYQLYSQQSQKQQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRD3</accession>
    <name>A0A8S1JRD3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040343</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRD3"/>
    <dbReference type="OMA" id="FPELEYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="797"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="903"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="817"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="846"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="858"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1097" mass="131028" checksum="45A808C7E370EA75" modified="2022-10-12" version="1">MIFLIDSKSILDCRTYSIIRPHQFDVVVILEYFKQQLQTAELKEEQKTVLFDKLKTLANVLLNSNKSQIIRLIQAFTYKSSENKQIFETFQSSSCSQWFLEQLYVDLVQIIFQQYLKNDQQFIGLFLKKIMNLYELDIQVQNGQYKYTEVQENKNENFNKVAQSNQKCLNNNEKKISQDQIYQFKSQIDDCIKENNLNQILEDDQQVEKNCHIAKNNIKTKKQSKKQIKDNIEKQKIQNFNQDTNLIQSNPPCSNQQGQGSSGLEFQKQYDQINDIISNIISKKSIDSENLTEEYPIQKEQLQKQDIQLTYEQIQMQNHDNQEIFNKNNNQNIKSNYQIPENDQQNIKISKYTSLEGVYELLENQYCFDSWWPEFRAECIKCKNKEDLFQILNQKAPILMEKWTKIAEKSDSEIIERLEESDQEKQNKQQLNDCLIFQEQDKNHTLEKSSNLESDKNITTNIVDKPQENIDQLQNNQQIQSQGNQTESNNVQSNQLLINQEFLQDNRFSQEFNDSGLFNLNQQQNFENPNFSDLFVLSPSKKSSVNYCSDEENFIINKDNEEINNSNQSQSDSVILDEKQTKKSLITNLEQFYKSKLVENQPQHIFPELEYMGDTQKMVEKLLFYLDFENLDIQIEDFLAQPPINPSKMLQEFVLTTYNIIKHITIHKYCTIIEVKQVEKEYLGSVIICQVNNNNKEIKKYKGPCLDIVNIITRLIAIKKLNNKFFSEGLESISLFQIVMSIIKRLIPGKGLYKEITEKYNKIKSPYDKNLDKIVIVKTKKQFSELSLNQQNSKLDKSEYKKYDSNKQSSSKRKNYDKRNDQRDGQYQIKNKERNQIKNKKADYYKTQNQNESNKTFQQNQNNYDDQDYSYDQSPYNYNQQNQQQYQNQQSNQFLQSSQYKTIPNSQNQPCQQGYQGTSKNNQQKPTFYQQSTENQYAYNMPYHPNIQEQFQYQNQYQYNNQNDMHMKTQPYFQKKSQKIQKGNYKQNQHYQYQFQTQKRISYNNQQNNQYDQFYYPQQIMESKEDYVKQKQRKYDYHQQNDFYQNQGQFNQQQFNQENQYFNQSSYSQIQPQNYQRPIDFQIIQKMVTKLMGDKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRF6</accession>
    <name>A0A8S1JRF6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRF6"/>
    <dbReference type="OMA" id="WWQAGKE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="83" mass="10200" checksum="EA019A5F04B83F5F" modified="2022-10-12" version="1">MSIDQILKDQEQEWWQAGKEDEYNVLNKIQRTSCRPIQRKYLECLKHNFDEQMICDQFKKDMDNCLSILQYMKIKEIQKKLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVG8</accession>
    <name>A0A8S1JVG8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP synthase subunit beta</fullName>
            <ecNumber evidence="14">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0060500</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 14">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="14"/>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVG8"/>
    <dbReference type="OMA" id="XERLAKE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18110">
        <property type="entry name" value="ATP-synt_F1_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18115">
        <property type="entry name" value="ATP-synt_F1_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01133">
        <property type="entry name" value="F1-ATPase_beta_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000005">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.170:FF:000005">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000026">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050053">
        <property type="entry name" value="ATPase_alpha/beta_chains"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184">
        <property type="entry name" value="ATP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184:SF82">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039072">
        <property type="entry name" value="ATPase_subunit_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 14" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 14" id="KW-0139">CF(1)</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="169"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8044416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="499" mass="53643" checksum="59484381507744D4" modified="2022-10-12" version="1">MLTRALAKQMRLGFSAAKKQAETGKTATTTTGQIAQVIGAVVDVQFDGPLPPILNALQVQGTSHKLILEVAQHLGDSRVRTIAMDSTEGLIRGQPVSDLGGPITIPVGPETLGRIMNVIGDPIDERGPIETKIRYPIHREAPSFVDQGSGAEILITGIKVVDLLAPYARGGKIGLFGGAGVGKTVLIQELINNVAKAHGGYSVFAGVGERTREGNDLYHEMVASGVINLSGGSRCALIYGQMNEPPGARARVGLTGLTVAEYFRDEEGKDVLLFIDNIFRFTQACSEVSALLGRIPSAVGYQPTLATDLGQLQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRALTELGIYPAVDPLDSSSRMMDPNIVGEKHYTVTRGVQKLLQDYKSLQDIIAILGMDELSEDDKLTVSRARKVQRFLSQPFFMSEVFSGRKGKFVSLSDTIAGFRALLDGEGDEYPENAFYMQGTFEDVIEEAKRVAAETTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVY0</accession>
    <name>A0A8S1JVY0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Letm1 RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0080206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVY0"/>
    <dbReference type="OMA" id="KIYHFIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="2">
        <location>
            <begin position="75"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="321" mass="38980" checksum="CF64B83FED722AB5" modified="2022-10-12" version="1">MIKNFRKLQFQVLNYNKIVTISKPKTMIDKIKVGGQLFFGTLNHGIKSCYYDYKLTKSFGDQFTAYQLYLKFHIDRELFKVFPFLFIFLLPGSVAYLPIYAYIFPNAIPTNFLFEDQYQQRFSKKLTEQKMAQRLLFQQQNIIKNFSNANQLKETFILKKNLFEQQFNLKNLNSDQLSLICQHFQIEYISLLNVPNKIYHFIFNIHNYIYNFYYYLINSKKRNLYQDPYKIINIGRPFFVEFIRQKVLIYQLDMYFEYINAQDLIFDYQDRNYYKYAIERGLTNGGLKLENYNGDWIRFTQQSQLTNTQKIWYSILYSQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWM9</accession>
    <name>A0A8S1JWM9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080385</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWM9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039245">
        <property type="entry name" value="TYSND1/DEG15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21004:SF0">
        <property type="entry name" value="PEROXISOMAL LEADER PEPTIDE-PROCESSING PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21004">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="228" mass="26077" checksum="0BCDCC00265DD440" modified="2022-10-12" version="1">MILASNFVQSDFNQIGSAVIINDKKNHISYLLTAQTFLVSNEKGSQILVGQNQIKAYLLENFQKFLNFTLAKCEEYLQSSLNIEDFIYYKELEVGQSVYMICYQEGLFQNKPFISKGRILKLHKNDQNDNFTIITDCYIKNSLNGGGLFSEQGKLIGILQNIVNYVSEGVFCELSIFTPILLFDQLFKDLSNQKQLTEKSIEKFGLIQKLNKLPPYVQSQLGQQNPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2Y7</accession>
    <name>A0A8S1K2Y7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Immediate early response 3-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0130194</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the YOS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2Y7"/>
    <dbReference type="OMA" id="SQHIKGI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013880">
        <property type="entry name" value="Yos1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15858">
        <property type="entry name" value="IMMEDIATE EARLY RESPONSE 3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15858:SF0">
        <property type="entry name" value="IMMEDIATE EARLY RESPONSE 3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08571">
        <property type="entry name" value="Yos1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8049031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="9077" checksum="BEEC3F92B04AA588" modified="2022-10-12" version="1">MILITLLEAIVLISNGFAILNEKRVLSKYGFDKVDYTRAHEGGSQHIKGIIVNWLMFARSMKIFLIIGNLIVILIEILSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3K9</accession>
    <name>A0A8S1K3K9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0110435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3K9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="382" mass="45274" checksum="56215E8A056CE941" modified="2022-10-12" version="1">MIYLKERSSALIQYVTQDHAAIAKESLNDIMFYGQSIKIFFSDYEVISLKTQLTKPGEFTQDIKTQEEYFQGGEETHRIKPDSTYTLAPPFDNIQISNLTKNSCQVSVLQQYLQDYGQIRQSKLVTNATKYMAILNKFKQISQNKNSYDNLCVINMIQKKIILKLAIKHNPPSLFVIYRYNLKDKKLRRYRIELNSLVYLPTPDLITEQLYIEHDDYLNDNNVEYDQVLKMVQMLYEHQQQKSGTRRSVIKNLDQVDSYMESDQDELNKSIMMEQDDNWQTPPNRRTETIYPKDNTKFNQIHSSESMNLSLSGMQNFMQGQSKKPNNLTYLPINNFDEQINKNRRFVEDQIQQNQQSYFQNKFIELPQPLLNRNNQRVNNYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K5B6</accession>
    <name>A0A8S1K5B6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K5B6"/>
    <dbReference type="OMA" id="QSIDYCT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="257"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="483" mass="56790" checksum="8BCEC94C8BAAD0B6" modified="2022-10-12" version="1">MNQFCSIPEHGVNVEFICLNQYCRDCRVFCFKCLQSEKHNSHMKDVHKLRDYGKLFENKSNTIFEDLERNIEYVNTLFKWLRDEMFNNNQLSQEEFEDLSIEQLNDYLCSKLQMESQKQSILGIICKNVDNLIQTLNNCISDLELSSLDSPNKKIKGELSTINYELINSIEEQQINSIAFNMESTIMIAGYESSTIKVFDFIDGEVKLNQEINLHKKSIYCVYHMKKTKEFISGSCDKTIIVFQYSDDQQWHLKQQLEEHQGGVNCLIVNSKEDTIISGSDDTLIKIWIRENDYWTCHQTLIGHQHYISSMSLNVTENQIISCSKHPQILIHQQEVKSKVWVLLQTINVTTWGRRLCFINDSVFTFQPERKEQLHIYELNSKTNQFQKTKNIPVEIGTCKMKRRGCFWFFPQQFISEKQILVNKNECSVNLILYNQDGQFITKQSIDYCTSGVFGRMTDDGQYLITWDDISRRLQIRQLKLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6M3</accession>
    <name>A0A8S1K6M3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TATA-box-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0170043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6M3"/>
    <dbReference type="OMA" id="VRFHIRL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04516">
        <property type="entry name" value="TBP_eukaryotes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000001">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000015">
        <property type="entry name" value="Transcription initiation factor TFiid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033710">
        <property type="entry name" value="TBP_eukaryotic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="23824" checksum="C745FE992FAC875B" modified="2022-10-12" version="1">MKNELQTMPDINERLEELIRSTHPPKIQNVVSTVDLGTELNLVVISNGARMAEYNPKRFSAVIMRLNNPSTTALIFQSGKMVCTGAKSEDQSLIAARKYAKIINKLGFPVVFKNFTIQNIVASFDFRKPIKLDEFTKDQQYRSVVQYEPEIFPGLIFRKITDQKKITLLIFVSGRCVITGGKKTEDLNNVFKFILPILDQFKKRDIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7Y7</accession>
    <name>A0A8S1K7Y7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BolA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0170303</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7Y7"/>
    <dbReference type="OMA" id="CLGGFGK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050961">
        <property type="entry name" value="BolA/IbaG_stress_morph_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46229">
        <property type="entry name" value="BOLA TRANSCRIPTION REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46229:SF2">
        <property type="entry name" value="BOLA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8050927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="13227" checksum="44DE3F7A74E86612" modified="2022-10-12" version="1">MNQIKYSFCKVRNLLLQKLTQDFTPEHLSIINESNLHSVPKGSETHFKITIVSNEFENKSHILRHRSIYQSIDNLKNDYKIHAIQITAKTSNEWQKSTEVNPTPSCRGGSKVKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8E5</accession>
    <name>A0A8S1K8E5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0130024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8E5"/>
    <dbReference type="OMA" id="DQCAWIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035876950" evidence="1">
        <location>
            <begin position="22"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="296" mass="33103" checksum="C53A37CE682FC30A" modified="2022-10-12" version="1">MLILIFYCLSLTMGLSPLVCGDVTQEKDCLDIINGIYQCKWTTNGCVNMKCELATPPCDQLIYENKACSTSGANCVSVSECSEIDNQTSCGVVKAGGMDCIWDTNCRIKQCSDYDQATCKESNGDNCIFKDGICQKLLLCSDIITEELCKKIDLDDQCAWINNKCDIFDCKSYTNSNECFSAFSSTDSCFNGATDNSSVKCMSCFSLTNQCDCEQYGLYGCKWQTQTGSCYRQKCEDFEDTITCQQAFDGLTCVWYNPMDQCKSIANANELDRQCDLYVFSKILIFGLIIQMIIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9J8</accession>
    <name>A0A8S1K9J8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0180081</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9J8"/>
    <dbReference type="OMA" id="GNKSERW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="136"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8051407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="193" mass="22356" checksum="85EAA147E2C84801" modified="2022-10-12" version="1">MISLIKGIWQKYFDPPVFKILIIGLDGAGKTTLLNKLKQLAKHPFIQFDKIPKTVGLNITEIDYMASNKSINKKYKVTYWDLGGSQTQRSIWKKYYGECHGIIFVVDGNKSERWDEVRNCFNDKAHDQVPILILVNNRQNRDSGICIKIKELIQNNTDRFLNIQEVNIVDNTNVVESVKLLHNEIGQLFQLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9T3</accession>
    <name>A0A8S1K9T3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0180383</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9T3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8051889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="74" mass="8557" checksum="7F276582029C8257" modified="2022-10-12" version="1">MALLVDQKNVMIQIPFNLMDAINVKLNVNHNVQDVKEDIVLNVLLLDGFQILIQDYVLNHVEINIMLEMNNVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KBN4</accession>
    <name>A0A8S1KBN4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0140362</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KBN4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="15"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8050182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="597" mass="70290" checksum="1CEDABDE8C82B751" modified="2022-10-12" version="1">MEKEKVYPGKKIGDYIFMEELGRGNYGCVYKCKNISDQSIHAIKVIEYQSPSFKGGIVGQLLKEEINSLAQIESPYVLRLEHYLQTTNRCYIVMEYCDSGDLYQYWLRYNRILDENKTLVIIRQILNGLMELHKNKIIHRDLKLANILLNKSQVKLADLGFCKRLKDNDVHDKLQLGSPGTIAPEIVNKESYGLQSDIFSIGCIFYQLLFGELPFNFHSTELYLKQIDEQQINFRRNGVKVSKNVQSIIQRMLVRDPKKRLTFSELYQFDLFKKKINENLQFNQIQNEVQNQINQDEEDWRSIINGKDQPNQSNFYDDIYKKNQDEILKNQDFTYVEANNGENLQVFFTNENGKDDRQISNNTIKLNQQIKARKYVTPLKEDKMVFDQIQKDGLQFKPIQPRVSSSIILFNEFKQSLNKLQFCEQLYNDLKEFIQSDQINLLIGRLMLLKSMIIEVDQLILKNEQNCKNQDNMDLKLQDLLNFPFSQEFNLLISKIKEKIQQNKKSLLSPEVLSNHTTDNFSSLLFQGLLDIHSYLRDETNKEPEIIRKKQIAITILKVQTCVNFHELDFSNIDLKKQFEKIKQQNIIEILQKIYVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDN5</accession>
    <name>A0A8S1KDN5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aurora kinase</fullName>
            <ecNumber evidence="12">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0200001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="12"/>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HPVDWWA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="34"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="789"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="362"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1117"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1295"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="914"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="818"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="823"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="867"/>
            <end position="869"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="918"/>
            <end position="919"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="932"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="10">
        <location>
            <position position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8052787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1443" mass="168160" checksum="6DAC692A7AC062CF" modified="2022-10-12" version="1">MSTGLVINQVKERILFFSPTVQGKMEREACTQDFEVIQGLGQGAFGKVFKVRHKKTKMIFALKQIAKKQIKQQKMTQQIINEVKIMYGLEHPNIVKLYNHYEEEDYIYLILECATGGQLWQKLNRVGRFDEKTVKQFMQEVMSAIEYLHTRNPPIIHRDIKPENILLDANGHIKIADFGWSNINNHQRTTYCGTLDYLAPEMILECGHDEKIDHWSLGVLIYELLTGKAPFAPSSQIKDQKESQKILEDNILKVKINFPNDFPSLAKSLVQGLLQRDPQKRFNIQKMREHPWFGNCQIQQEVQSEQKVKAENIKDIIKDDKAQFSKEEIAKVVNRQLNQTKEEDDVIIVVEDQKEKEKESTHDFQKNTVQLLNNKITDLQKQLNDSKILLQVKIDEIKQLQQQQQEQPSLLMNVDTRKIKLLEEEKTKWKQAYEQIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSNKYRNLFNDYILCNNKFSEEQMSTGLVINQVKERILFFSPTVQGKMEREACTQDFEVIQGLGQGAFGKVFKVRHKKTKMIFALKQIAKKQIKQQKMTQQIINEVKIMYGLEHPNIVKLYNHYEEEDYIYLILECATGGQLWQKLNRVGRFDEKTVKQFMQEVMSAIEYLHTRNPPIIHRDIKPENILLDANGHIKIADFGWSNINNHQRTTYCGTLDYLAPEMILECGHDEKIDHWSLGVLIYELLTGKAPFAPSSQIKDQKESQKILEDNILKVKINFPNDFPSLAKSLVQGLLQRDPQKRFNIQKMREHPWFGNCQIQQEVQSEQKVKAENIKDIIKDDKAQFSKEEIAKVVNRQLNQTKEEDDVIIVVEDQKEKEKESTHDFQKNTVQLLNNKITDLQKQLNDSKILLQVKIDEIKQLQQQQQEQPSLLMNVDTRKIKLLEEEKTKWKQAYEQIRDELHEKQAVNLKLNAEIEKIQQLTKSLEKQKADKQLLQEKIKKLQEEIDLKDQQILELRTEQDGRVQNSFHGSFLNQSTLEDGKSVADLNLTFQEMKNSFQIAREQIEEFDKAQRRIVQQEAEILALKSQLNEYKESIRITVQEQYEDQMEELEKKQRDQIRELEAKHFNEITKYENDKISLRDQFNELENLKSEVILLKKDQEQANLIIMDLKKQQEIDKRLRYQMEQRLKEQDILILDLKEKERVYRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KGD8</accession>
    <name>A0A8S1KGD8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210401</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2609"/>
            <end position="2630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2703"/>
            <end position="2724"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2736"/>
            <end position="2760"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2787"/>
            <end position="2813"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2863"/>
            <end position="2882"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2894"/>
            <end position="2913"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2925"/>
            <end position="2948"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3046" mass="355216" checksum="DE5C352CCE3B482A" modified="2022-10-12" version="1">MNSISHIVSVTFLTIVEKYRLCDFQQQQCSVEIQLKSLLLIQDIQLETIVECYYENDDSILEINETRPQKYTFTLETNSTNLNAKGFGFWSKYIHNRGLTPQYQGPMPFYDPYCQSQDYQFQGILLMTLLDSQSNLFAALILNMKDDPISLTHDFYLFPIGPNQLQFVQMSFDASQYENVWIYTSFVYSMDDQTIRLFTNIGSQLQTLIYQIISDKITIELGGSLDLSQNDVYGMKLYDFKGYISPIQEYDQFQIMDDFFDNLFSKCFFQEQQTKAEISEFKYFEPQIFEGTAIYFNYHETIINNRYILKCWIKQDYQEAYDFYKTQYGEEHMQLMQAIFRIDSPFGINKHFVGDAVARFFYTVNFQNNNQTNIHFQTEFYKIPILTPKYQDDSLRDFDTLTINGYNLYDLTQKWHYFILEYGRTPSQNGGKLQLRFYFQDEEPQIYNLGTYDYNSQFTGYFITLNLQFYEEPFRISRTKLEKIIFITGFQGDNEDKQDCYKSCKNCNGPLNYHFTSCYEEYNYFLTQFNQCECKYLHTYNENTNQCDKIDDQHGLTIQQESVSKFCQFGYFQVLYEYEYFCIQCPQSDIVNFFCGDCYFQFSTWYLKPVCTFDLIQEKNSYAYLKHFRLNSQIDVYYLNSELELQILEGASDFCEDTLHGCQLSNNYHLNQQIKIRCKNNHFFNFNSVCKLCDPYCINCQSEHVCLECIESHYFNIRTRRCELCSPQCLTCKTDLNSELGEKCLSCVQSYTSTHQGQCQKCGINCQYCKQDYNQQNQQYFLRCLKCFDDKIMAIRFNGIDCLIIEKSHCQYVMIISKSQSIKYNSYSYNFQPSNDLSDELAVCALCDPNYEYIVSTKKCQKLVEEVTCLIGIFHEFPGDLINTGSQRTCLVSKYTYGALSFTGEKNCQNYVSNCNFCFIENTYSPNYCLECFDGYYINRLGGQCLRCPELLNCETCFQQSVGYYDQWKSKTAILNQFLKMRDDKRFFFASHDFSQNPDDYETICISCIEGYKLKNGQCIKYCDSTCQSCIFKNDQYFCQSCGKNKYHNQLSVIQYQCSDCPSYCELCRERTDEEIFNLNSLFIKTNQNQKFTHQCLIPFLNNENIYYDQNFGQFISCEDFLQCENVLNIELNLFCTYEDYQTQLSQIIDQNLQKEFQMNNIVFHSLIQQNIESNSFSFHETNDIFETLNKLNVRRIKIILKSQQQQTCSIDQFGYISQKFSKYVFSAIDIQLNLISEIEEPLILEIVDDLSFVDFSFVRMENIKISIKQNTNSKIINIYGFQSITLQLEKVIITSYLQIKSTLFNFQCNQLNSLLFNQVHLENIQIDNENLRSIFSFEYVKYQNQISIENFQISNSNITFTNIFQIISSGVYQIFLNNVRINSEFVNCSLLVTSSEIYVFTISNMFITGVMKQTYPFISFQNVVIAEIDNFSIMFMNLIDSTLLQLEKQFRITSFVISTCQTQGYVKFIQNDIPKQSISTHLQYSMMYLMIEDLNSSYQTQILDLQSFSSTTSGLEIYNIMLQNNNLNEFQQEPYYLFNIQLQNVKIIDCDIIRGNGLSEFQFSNVLFLSFINLKITQSKITVLHQYFECSSMQQFKNQHPNLIKVMDVINVIFDTLIIERINILNSGLLLFQKQSSTLQSSEDSIVFQNLLLKDNIIITSVNDAYASLISIMSKQKIVVNIIQATMSGNQLHNYEQNLQMPSAVSLLIDCPSGSINLQNNYFYNNFATNTPDSIINIVAKQIKFDGIQFINNSIYNIYSLQNNIVYGFSNDQVIFYSNLQSIFQLQNYVGNAYFSADSVIGSNTLIKNSQGKNGVGLYIKAKITKLNDIQFLNLKTFFYFNEENGACIFLEIPSSDSQVLIQNIIAYNITSKDYGGLIYIKSDYDNLNLTLSNLTISQCISFKGTVLHASFLNNTLSNRITIKQLYITNQQNAFQNYIRQIVENQIILNKLNERVTVYLENAIVYLTDIIIKNLFGESILLSLYQQNAILNSIKVLNGTILRQKLIQMQPKTNQNTFILIQTLSIINIQEFTKPQTQCNVSYVEKKKNYKNMQCQVINQKPLNNLIVDIQQDCSKEIECLHFLFISSAQIDQQAIIMINLNTESHIIELSEISLINNNFSNSLNGLIFIQWSEIKQEDLQISVKKLSIKNNHCGKVGCLYINSIVFDENKIISTKYNRLLAQDYEKIVQEIRHDINIVNYVCIGNLADYGTCLFSNHTNIQIRNSLFYNNTANKIGGTIYFIGKQSKLFQIDCQISFNYAQVAGAIYFDNSQAQDMNQSKTILTDNRATQYGNDNVQAPLHLSITLNDNNKFFQTFKVDESINYLKEQVNSLNKNHIIFLPSGTPIGLYKKYNSEMQILEQAELKFRILALDNQFTQIKNLKNTQCNIGTKVYNLTSKTYLEVSKSSYISKNSVIFNEKTNDYNLDDMIIYFDNNNTGKSYLQLIITCNSIKIPQYDETKQIIQQFHNNYQLIININTFKCRVGEIKSLSNQSCYECDFNLDQYSITLNSNKCNIRDEQTTISVTSFQLNLKQGFWRPYFDNNYIENCYNFNENCVGGWKYGMDSCFLGHYGALCELCDIENIRGDGSFSTAQEYSCGSCDDIRFNIVQIIAFSIWTLFTIVLSVKGAITTENSFLESPFLIKILTNYLQIISSLTSFKLNLPVNFFYFLNGVGNPIQRVSYSMDCQLIQMTSLDIHYGRLIWQLLLPCIYFLMLAIIYAIFIFMKYIKYKSPIIMTAVIYMYIYFQPTLIGSFIAMVSTRTIANVPWIQAYVAYRFDTVIHNRWVLILCVPMLSIFGVIIPFGLLLCLVYNKDKLQYKRGKSVFGYLYYEYKPQTYFWEIIKIVTKELVIIFLIFYEDSIILKGSLIFFLLLCYWSLNLKFIPFRTSRLNLLDYQSSIVCGVSMILGICLNMDQQQQFQNISIVLFISLIIINILMLIKMLLYILNAYSREMVNSLDQIKLTILSILPKRFKSNNSICKKILKTQAETNQRVKANILKLKAALKKHQILSQKPKVQIINPIFQKENLLILLDSKNKSKQNKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKW0</accession>
    <name>A0A8S1KKW0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0200372</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKW0"/>
    <dbReference type="OMA" id="WAGKMIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000526">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000587">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000771">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000660">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="300"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="336"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="452"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8053522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="57050" checksum="174412E8A00A5F33" modified="2022-10-12" version="1">MGEKRIDKYLFNPKDVIGEGSYAQVFRGRDEKTGVKVAIKVLNKSVINADDYLREGLISEIKVMQKLKSPNIVQLMDVMETNNNYYIIQEYCDSGDLDKLIENYVAQKKTMPEKDAVKFMIDILNGFIQLIKNGIIHRDLKPANILIDKGTFKLADFGFAKCVDNFKKDMLASLVGTPLYMSPQILDNKKYTSKTDIWSIAFIFYEALFGKTPWTARSPQELLKNIRSQPLQFKGPQISKEAQEFLTGCLQAEEKDRLSWDEIYRHPLFKGHFTDFIKNVSILEDKATYLINDIRQMIIKEQIDIGELFAELDMTKDKALNVNELGKFLQRVDKNLTREEIEYIFNKFDDDGNNLIEFNEFKKWLEENDCRMTASEVSRTKKKVSILMKPQQDIQAGSLDDRARSVIEKLKISIVKYNINLLDLFNKYDKSANHELDVQELGKLLKKIDQTVTDEEAKAIFTFFDHNKDGSVSFNEFEFVLKECLVKQKIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KNH1</accession>
    <name>A0A8S1KNH1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0250070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KNH1"/>
    <dbReference type="OMA" id="NEKKCCY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="48"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8056818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="207" mass="24731" checksum="C29ADC34C6906BF1" modified="2022-10-12" version="1">MIDDQDKQQEDDCMNQYCRNEQTQVWPAKFSNQTLYFCQECLNLYNEKKCCYFCAQVYSEDNQNFLDGQKWIGCDQDGCEKWTHLQCESKNGISQIDILVEDTKYKYICPWCRIEDQKQRFLKAPCRIMNKKALKYLEKMNRRQTNTNEQPQHNTQSNVQQFYYCPSASSKQSYLEELLKKNGGFCQQVTQEEIQVDLQKMISLMKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KX30</accession>
    <name>A0A8S1KX30_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270222</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KX30"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="80" mass="9359" checksum="0B5855F25918246A" modified="2022-10-12" version="1">MKNNKGMKISPTLGRISKQIPQKFGFSICKITIHMFEKINIQMANGYGMYVKGLRNGYIVLIIQVIIQMWKDFSTQIWYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYH6</accession>
    <name>A0A8S1KYH6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0300201</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYH6"/>
    <dbReference type="OMA" id="WGLIFYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="234"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="303"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8060569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="358" mass="42852" checksum="5B0256E7B59191A0" modified="2022-10-12" version="1">MQDQIQQDLLQQHNQDDDEFMIKENLDHHKQPTVLFQIGKEFLQQHHQLLAIHTVLKFIAALTLIILKVTLYRDQILQKNYNKIFDDAYLEIWIYVSCIHSLFEFIYCNQLLKKIVNEILCIQVAEKIGQLILIKIPETYSYDEKQSYIKDQVEILNQGMLQRYMVEGRIEQSIKVLIAKEIKKIILQTEKFLYLLGKGCFILFQFILLWGLIFYFHCVNSNQDLEIYTFYRIYLFWVVFLGIIQYFNFYIKVFLFFILLPINFYDCLNNYCIKRKERMQINDQFKDSTLSLQETNEENNNECAICMNKLVDKDEIIIITCLNKHRLHSTCVGQLDINNKCPLCREYDGETRQLSMIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0G3</accession>
    <name>A0A8S1L0G3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8060937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L0G3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="257" mass="31389" checksum="E6B5ADC9E8E0BB2B" modified="2022-10-12" version="1">MHQFINLKKNLKNMLNNFLLLFNKYCNLDHKQISINYLIDLNIIQDCNNQDRNLVNINYLINLNIIQLDKLNNEQQQLNKYHNQYHKLVDKFLLLNLNKFPSCKIHNWLLFLNKQHNQHHNLNHKHLLINLNIAQKNNLNKNQYKFSTLHNLHHKRNRKNKLKDLNINQFHMLNNEQQLFHKLHNLNHIKININCLTNLNTYLNCKLNNKQLYLDKQHNLYHNQISIDYSINLNTLNHNKRDNQQQLFRKFRNLNHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L859</accession>
    <name>A0A8S1L859_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L859"/>
    <dbReference type="OMA" id="QIMKKQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="141" mass="16809" checksum="03C70F5894A3DC77" modified="2022-10-12" version="1">MNFYALDFEDQNDQALEEQEQIQISEEQEHEFDQSSNIEIDKNITFDGYWQEEPVLLFNVEEEVPKKKQKSIVRKSKKIKKSDTETDVQSQKQMPKFKISNDLAKLQQCQMLKTIISQMETILQNTRTQILNQIMKKQYTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9X5</accession>
    <name>A0A8S1L9X5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0360060</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9X5"/>
    <dbReference type="OMA" id="VWNEDFY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044571">
        <property type="entry name" value="P4KG1-8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800:SF11">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE-RELATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="31"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8064307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="575" mass="67165" checksum="3EC332184B067410" modified="2022-10-12" version="1">MKQGQVKRKFESFGFSSSNPYDPRAQSELVLHISNQVQKGNDFTIKMYSWMKISILKQKIHKTTRLDLADFRILYKNQELSLNNYDLEDYGIKNHVTLKLMARKQGQQLYFINSIHNILEESKKTQEIFQNAKWAVEGAFMKGITPKLTDFGTQGTYILEDLNHKPVAIFKPYDEEAFAPNNPRGMRAKMNSPGLRQGILSGEGVDREVAAYLIDQSSGHYHNVPITNYVQICHPAFHEAEEYKQFQYEKIPIKEGSFQLYIKHDDNVGNYGSGLYPSKEARKIAILDIRILNCDRNEENILVRKKKMNQPIGQTRQAYDYILIPIDHGYAYPDCFKICRDEVVWYHWGQMTQPFTLEEKSLIEKIDPIKEIQILREKVKLREICLRNARISTTLLKLGAQSDLTIHDISEILYREDPDELSPIEIAISKAEHNYDNNIRVPKGYSNFKEKIFSNNLIQKSLEFDQSTLENQPRPIQGQPLIKLEIIKDEDENKNLNNDKKIKNTQQIQLSDKKLARVGSQQEIKKCPKPIEKVWNEDFYSHVTFFLQQIIDSKIQEKFQSPRKYRARYISEEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9X8</accession>
    <name>A0A8S1L9X8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoenolpyruvate carboxykinase (ATP)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0330236</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9X8"/>
    <dbReference type="OMA" id="QILHPKN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004612">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00484">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00453">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001272">
        <property type="entry name" value="PEP_carboxykinase_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015994">
        <property type="entry name" value="PEPCK_ATP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00224">
        <property type="entry name" value="pckA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006820">
        <property type="entry name" value="PRK09344.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006821">
        <property type="entry name" value="PRK09344.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01293">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006294">
        <property type="entry name" value="PEP_crbxkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00532">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8062632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="552" mass="61586" checksum="E0D5C8347CD40845" modified="2022-10-12" version="1">MLHHLWKYTYNTSKALPLEQLTRWGLHNATVVRNASTPELYEIAAMDPLSPDPETRQGSISSTGAMVAYSGRRTGRSPKDKRIVIDEHTEQEIWWGDVNIPIPKKSHNLLEQIALQFLNTRPRLFVVDGYAGWDPHSRLRIRVFCTRAYHALFMQNMLIKPTNEELKRDFSDDVDFHIFNAGPMSAPKLVEGVGSETCVSVNLTDKKMVILGTQYAGEMKKGVFGVTHYMLPKQGILTLHSSANEGVNGDVTLLFGLSGTGKTTLSADPKRKLIGDDEHGWSDYGIFNIEGGCYAKCVDLTYEKEPEIFNAVKFGAVLENVEFLNKNSREVDYHNISITENTRVSYPLDFIPGAKNPAVGSHPKNILFLTCDAYGVLPPVSLLTPEQAMYHFISGYTAKVAGTEMGVKEPVATFSACFGEAFLPLHPTLYATMLAAKMNEHGTKCWLINTGWSGGKYGIGKRMSLKNTRSIIDAIHSGELAKGEFDNFEVFNLQIPKKATGVPDQILHPKNTWTNKSEFDRTLRNLGEKFKKNFQKYQSKASQDVINAGPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBT8</accession>
    <name>A0A8S1LBT8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0360162</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LBT8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8064509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="87" mass="10523" checksum="E78B9C4036A5DD71" modified="2022-10-12" version="1">MNFYFFIQLAQNLDNFESFTTYKLLDQPRTGGLKSDNIFEFLNDNDIQITQDQLDYTFRALDEDSSNIFRALFWLKQLALKEQYYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LC92</accession>
    <name>A0A8S1LC92_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LC92"/>
    <dbReference type="OMA" id="WIKVSTA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="351" mass="41029" checksum="A6FB546F32A7F4AB" modified="2022-10-12" version="1">MKTASIFLTDNQQSQLQFSNRQNRNLFISDFNFETSRSKKSGQSISPKKQWIKVSTATESLHLIMTPNLLQLQNFNSLKKIFKRCVIVVRACIRLQLLQQKKQKKPITVFRNPLNGKESLRDTVLNETVFNWCRGICKKSLSSIKMPVFDYLKNPNIITKADISKMQLINAIKFIVESLAYYTHPHYIHYDLKGFLCSQLYQDYKLYFTQYVSERTRYKIAILDELQEKEAAIIGMEIIIFNLAEAIIQDQLSCNFSKRILISVLQLLFKQYFDEMNCSICNYDCIQGDIKITNNKLQLTFKTQHSGSFNLIDGVYNDEQITSLLNFNRSQQGKIQEYFKIAVKHLEKQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDB5</accession>
    <name>A0A8S1LDB5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0380073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDB5"/>
    <dbReference type="OMA" id="DMQAASQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF197">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="35913" checksum="28716C31C3B69412" modified="2022-10-12" version="1">MKPSDIIAICFFSLVILLYLGLLVYLHCFKKIYRLIFPSPSPMYTEKFFGDKLKYANVYNSLVNLKSDQLLNSYNLERVRAIPYVYLKNKYSETSNYLIYFHGNAEDMQAASQFMEHLMKTINTNIFVIEYPGYGVYEMQKNMSSNLIEEDALTLYDHIKKTQKLLDNQIYVFGRSIGTGPAFYVASVRNVKGLIVMSCYKSIKTIIQDLCCGFGIIIVFFFCLPDIFKNYERSQQVKCPIVFIHGLHDKLIKPYHSNYLYTNLPMSIQNKSQIFIKERMTHNDFDIDNDIACSILELFQELRYSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFB1</accession>
    <name>A0A8S1LFB1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0390094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFB1"/>
    <dbReference type="OMA" id="MGKFARN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="172"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8066450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="363" mass="42296" checksum="43AE58E27B2E686D" modified="2022-10-12" version="1">MQVKRVVPNPIDNFHTEPRSSQQSCHVTKSKPIIPTTPLQQSKPNNLIQSLNGLCKYFNQKEETKQITYYAPKSMQRSSASLSPREIMAANIQDNSPLLVKQLLYGKKDQKENFNPKVGMSIVEAKQVPHLSINQDQKLVKVNDSSHKKSMGKFARNQKSRNASVQYHEDWKLKYEELQQSLCERIQQLENELEEMEKNRKIDQMHSDQQTNQYIEQLQYIISEKDQVIYEQELKNEKLQNIIDMQQQELQKFKETKYKINDTNEIQRLQQIDQKFQQIKGHLPALNITLRNIQSIFDAASSEQQINNLSLSSVSMVELINEFNQGVEENTQKSIKNNTILTSNLSEPKPINLSEILNRKRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIP1</accession>
    <name>A0A8S1LIP1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0390187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIP1"/>
    <dbReference type="OMA" id="CHQSQLF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="152"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="555" mass="65330" checksum="D014E9B38C0571C1" modified="2022-10-12" version="1">MKQNKVNKIDFELLQKIQINESNVNLSWVLKFPNYHELQSFIFQMPFIDLKILQSKYDIQGPCHIITNNQIQFPLLQIAEGILIFQKYKCLFLSDCIIEKQKFVCHQSQLFLIKISHNQGRLVLIFVHPVSQREWFKILKLSGKQNNFLQKYRINEVIMSNIFSVLLKRKNKKYVAQIYKKNDIKSKEQNEIINNYIKILKSGKIINYYPVLSIFEDTETISIITYQFIGKTFESLLLSEQTSITQMNLAFIICSVLQCLKCLQDEELFHGYICLENIIMANILGSLQIYLINSNYKPYNINNLNFYLNSIPNYLIAPEILSLQDQPSVKSDIYQLGMIFLIVSFFAKGQSYNRQYISKIFQSRVELIKQQEINFQQNKNSDFPYLFSACQLDLIKKMTENDPNKRISVNDALKHAWFINTKDKLKSSKQQSINRNLPSLKTIIEMVEQSEYDIRRKSSKLSIIQQGKNILECATSLQQFDFNAFQQQQDANYVVVKSLESQNEIIDEDHEISNLIFKLNDKQFRMMPSLLSHFIQDKNQDRLTYPNNHLTQFQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKU6</accession>
    <name>A0A8S1LKU6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0400021</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8066921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LKU6"/>
    <dbReference type="OMA" id="EHITNKR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027512">
        <property type="entry name" value="EIF3A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054711">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3, THETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22591">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PCI" evidence="7">
        <location>
            <begin position="322"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="785"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="576"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="785"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="827"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="868"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="896"/>
            <end position="938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8066921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="945" mass="111601" checksum="BFF8425C6D59A186" modified="2022-10-12" version="1">MASINYLTLRPEQAYKIYDDYVKEGKNNDALLILFRYLSSFGQRTRKTTNSYQDQGDVSVASNIQYLYKLMQAYIDLRLFVGVNQPDEQNQLKEVFKAYRQMTQHNHLDAFSDILKEYLIKVEKIFSDKVKQYDVEELKKLPFVGQLSYEEPAEELLYLSFDPEAKQQKDQQKSCLRLLWETYRTVMENVKWNEKLEDVYFEILKKVSEFCLKYKRKAEFQWFCDQTNRFVLDLNDRELDIKRNPTHVDLTKPATNDKHVNAIFDIVKTAMALDMQSEASKTLETVKVLKSMRRGNFKASYLMLYYDLLSQIFQKSGNSLFQAFAYYNHYLAFKKKPVQTQEEIKSKNAEINEKLSHLVLSVLVIPLLSADYNQRDKTTSLFEKGTKIPTRDDLLKILGEMNAVKFVQPIIQKVYLLLENQQDLLTVSDQANQVFNELRQNEKYQLFIPLLEENLVGQVLKSISRIYRTIKLDQLKELLKFTNSETIFQTLIYSNSNKFISCKVDFQSQIVSFNSNQISNDQQILPTILLNLQPQRVIQDPIEQAKKQLDSSNLVILTRQQTLKNLNRLADPDLIKKKVEEDNQKVIQEMKEQRRQDEERKKFEQERIAEMEAKKAKELAIRQKLLQDIIKLKGGKYVTFEINGERKKIELLKDYHLLNIDTGILQDIKKKFEEEAKKGADDKYLRTYKKNDYIERERAILESKIITSWPEDNIEEAQKKHEQQYQANLKLKESLTDAFKYHDEIMTGKRQQKEKELQEKIEQFKAELTQKYKKIIVDRAQQLLKQDEEKKLREEEARKKEQEEKEKKLKAQQAEKQPVFENKFARSSQTSQQQSSQQQPIQQVTQQSQPQAQTQTQTQTQFGRSFGGFSNSKPQNEVQQSKPVQQFSRSTASSGQQQPQEQPQQQQPQQQQPQQQQQQQQQPQQQQRQTQQQTNQAQKFQRNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPD9</accession>
    <name>A0A8S1LPD9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0400183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPD9"/>
    <dbReference type="OMA" id="EWFENMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8067243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="36622" checksum="81253164C529646D" modified="2022-10-12" version="1">MGQECQKCKAFSAKEEFDIQNMDEDFGDESPDVEQSFLDEEDEVKKHNKPPSLDNFNKRRMFRKEDTYDQNDGKLSAKIENREIALTEELRGEEMILVDGSKYQGNVVNGKANGKGKLWLNNGDIYEGDIVDNIIEGNGVYYFHRGPIYSGQFIQGKANGMGKETWSDGSIYEGQFRNGKKHGQGIYKWSQGCWYDGEWFENMIHGQGRYEWSDGRYYNGNWNKNQMHGRGQYKWKDGKVYDGEYEFDKKCGFGIFQWPDGKQYQGYWYDGKQHGKGVMINKEGKKKFGEWVNGKLISCSEEINVQIIPDGWADKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSC3</accession>
    <name>A0A8S1LSC3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0420146</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSC3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF72">
        <property type="entry name" value="OSMOSENSING HISTIDINE PROTEIN KINASE SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="370"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="654"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8068413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="777" mass="89839" checksum="B5FFD10D7E31A1FF" modified="2022-10-12" version="1">MAKVIVQFRISTILQILINLGFIINSAVVYNSSDIVKFRSIFIGWMLICLFLQKISRKYWNITTNILIFINSVISVLLFAFFTKFVNTAQTNIDDIMSLSLISGLQQGLFAMSFFLIQSNYIMQSLTMISFFLILMGIFTQFLNYRLWTQYLLLIFTCYLLRQNEKTNRLNFLLIQKSHKNLEACKKLYDETVPTSIIILEEIVQQQGVNNNDVLNSVLTQKEKLYTLDNRLMNVAYFNKSASIHFETAEEDILTERLAEIEIVNSEDSILNCEKNKLLDKIQGLQSTLQTEMRFNTINSFDYQIMSTNKKMQCQRIKQSKVHYYDAQAQGCLWDGKQCIMLILNDTTDRVLRIKHLQDLDNYKDNLLAAVSHDLKTPLNGQSILANLIKTMMENKSFIGRNDIQEIVVHIDDMISNQQILLTMINDLIDYSQLKKQGLRLNYTQFDLSACVQQIKNMFKRQMDLKKLQFNVIGLNEKVIMYSDQIRLQQILFNLISNAIKFTYNGQITLKINKINSGIQQLIHFSVQDTGIGIPIHIQNKLFKAYSTFNLGNQNSQGVGLGLVISRNLVGLLGPNEFIEMNSVENKGSNFSFSIYLNAQHRECININTLKEDNGSPDNDPSPIHRMPNPVIKRQNRKYQTVVDMSTSLYQRLKILIVDDTIFNIYVLKQLLKQIVIQCDIHEAHNGQEALEQVQSIRFDIIFMDINMPILDGIQATQMIRQFELNNQYQRSKICMLSAFQGERDLKQSLEIGADLHLSKPLQFQALKTILKQLKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSR5</accession>
    <name>A0A8S1LSR5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0410160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSR5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01197">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="283"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8067776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="337" mass="40409" checksum="768974F0D6A4E7E1" modified="2022-10-12" version="1">MINLKEEQRIEKIKLVNIFGQHFKYTLILLIINTLIRSQGSEQTIFSNEQPIKQYNHLFDVAYLEIWIYLSCSHSFFAFIYYIKLLRLIDDSIQSQKVAKEMGYLIEFYIPNSFSDQQMQNYIDEQIKIQNQEIFIRQRINGQENDSRRFNQISNEIKKTLLKNNLYLNLHGKSCFIAFQFIQLWCIIFYFYSQSNKNQGEMYKFITMYKGYIFSVVFIGVYQYLQIYIMVLLVIIFLPILLIYSLYNWLNKHFNKKYKQVKIDDSLQEALFSSLEIVDDKECSICMTQFLDQQYIVILPCSSTHRFHSSCIRSWLQVNNKCPLCRSEVTIYSEDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTJ7</accession>
    <name>A0A8S1LTJ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0480147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTJ7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="212" mass="24786" checksum="73B24410FE54FCE7" modified="2022-10-12" version="1">MFSKPSNIFGYDSYFNSPVGSYCNSPIRSLDEEIWMQESNQQPIYPEKKKIIDQKSEENFEVYSGTTKIKNHYSEKMKPLINNTQGEISTQQEKEKEIPQIKKLISKKEVHMLPGESKNIPKNYTRALKQFIISNFDSSVLQNADIKKFLATKPEKACKQTLENSLKNCQQLKQISQIFFGNLQWGKTFLEENKVELEPYFRFNSIWFGSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUU1</accession>
    <name>A0A8S1LUU1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0390046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUU1"/>
    <dbReference type="OMA" id="KILQLWV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8066354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="152" mass="17926" checksum="EF047ED38C8671AF" modified="2022-10-12" version="1">MGLCITKPITKKPKSLIRLIKVENENQNSKPTTLPDLYQKILQLWVMVEKKLNKLVHCDRTHNILELKKQIQNILNKQKNNVAISEITIGIIVNRMEKIAFGIEELIREELLNEEIANKFKQFYTQILELSLEYHNWKNIQNTDKTQVQSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LWM9</accession>
    <name>A0A8S1LWM9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar protein 14 C-terminal Fig4-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0450008</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LWM9"/>
    <dbReference type="OMA" id="MYQHASA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001191">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026825">
        <property type="entry name" value="Vac14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021841">
        <property type="entry name" value="VAC14_Fig4p-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023:SF0">
        <property type="entry name" value="PROTEIN VAC14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023">
        <property type="entry name" value="TAX1 BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12755">
        <property type="entry name" value="Vac14_Fab1_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11916">
        <property type="entry name" value="Vac14_Fig4_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Vacuolar protein 14 C-terminal Fig4-binding" evidence="5">
        <location>
            <begin position="500"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="374"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="551"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8070001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="733" mass="85774" checksum="CB784EB75CD0202B" modified="2022-10-12" version="1">MDDNVVPQQIIKLLQDKSQDKKQLGCQQLKQLVQSYAQSGKELLIKQIIETLKIKFTSSSQLYIKRQGMFALQTVAEILVKEYPQLAERCVKDLTQPILECLNDKEDKVRQYAVECLLQLTKIMKTMILLNFNEIFDYQLGRSAEQENQIVQAMFLLDQQLKNQVQIAVQEKFLDPGKLCYFNLNSFMIQLQSKLKTRVTYVRQFLLGWIKFLNQCHNNDLYIYFPMILEGIFMMLGESNKEVRNGADLQANEFLKQVEIKVNLDQRVNEQIIEILLKICQMKGNQNNYAKLNSLLWIFEYLRVFQQELEEEQKGKLGLQHSPSHVRSEEYLRSNTSPIQKNQMQQYLGGFESPIRKTILNSLSQILGPILILLSHEEEEIRKAAQKTNELLLKVMERVKNQSIEFINIVPTIKEMLTDKKSNTAESALIWMKHLLEIYSESLFPTIEDILTKLIDKLADSESAIVQNIMDVLANISMHSQYFEIVIDKILIMLHKIQEQSEKKGDLIFKKLCSLLNPQKVYFTITAKLLEMYSNNDVIFISNTVQILTNLLISEKELQNLRNILRNLKHEKDEKQRQHNQEIFETLYKTFCYNSMSVITLSLLSEEYELAYKIIISFSEVEVNECILSEIAQLINVLETPVFIFLRLQLLEYDSHPFLMKCLFGLMMLLPQGSAFNTLRQRLKNVSNTQIQSLQQDKNMETEEFLDISKLLSQFREIRLQCINQESKQKDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M057</accession>
    <name>A0A8S1M057_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide methionine sulphoxide reductase MsrA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0500114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M057"/>
    <dbReference type="OMA" id="QYRSIAF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774:SF1">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE MSRA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="1">
        <location>
            <begin position="6"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="181" mass="20986" checksum="48DEDB5B50493E2C" modified="2022-10-12" version="1">MNILNKATLGGGCFWCIEAVYKRIQGVSDVYSGYSGGALKSTANYKDVCKGNTGHAEVVQVLFDQNKVDYKDLLYIFFASHDPTTLNRQGNDEGEQYRSIIFYHSEQQKAIVNEVIKELQKEYKNPIVTQVVEFQEFYKAEDYHQDYYDNNQNEGYCRAVISPKIEKIIKKYKTQLKPEYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9H5</accession>
    <name>A0A8S1M9H5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Adenylate kinase active site lid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0570086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="SCTMLIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="2">
        <location>
            <begin position="145"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8076847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="226" mass="25359" checksum="D77C15D471107AD4" modified="2022-10-12" version="1">MKNLIIRYAASVAKPGQQFLVLFGAPGVGKGTFAKLLIKDTKFNHISTGDEIRKILKGNTSQSFDQKLIVTIKDIVAKGGLVSDEIVMNILQEKLKEPESAKGVILDGFPRTLRQLDLYEKILPTNVVVNVTLRADILLEKLAARRTCVGCGTAFNICDINRDGYVMEPLNPKKEGICDACGGKLVVRDDDKKEVIEQRMKEYEEKTHPLLDRYRXTQLQLYLQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9W7</accession>
    <name>A0A8S1M9W7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0530002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9W7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016755">
        <property type="term" value="F:aminoacyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030903">
        <property type="entry name" value="CDPS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04539">
        <property type="entry name" value="tRNA_cyclodipep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16715">
        <property type="entry name" value="CDPS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="398" mass="46177" checksum="BE8E646FB838DFA7" modified="2022-10-12" version="1">MSVYFNFERVQILLKWAYNNFSKVTILDFTELWRKTLTSQGIDIQKQNEKVKKQEKAFNKNVKMAIKSVLQEKNPLNDLEMEKIWSDLRKKGANLQSQEKFLISKVEDFYNNQAYLNSVQILQEAANKSSTLNSFFQEFTLSKILNIMNQNQKKLNINFAMDYFIRELPFFLNGKEIFGVESVFVYHKEFSPYYRILNGEFSDLGLGVSNGSGFARITLINEENQQLDSSIKKSAIQEITLENLQLDQNKKKNKNFRINQAIKEHFSILGDAIVTSLMSLAEQGIAFQAKNQQIKIWIEESRAYKNKTYLLIIQLKGLSFQLKTQAQVLLSSFLGTNITFNVKSNREQIKLSLVNKSLANLFSFDEINNIIAGLDDSTNDQFQQEFEHIKDSQIENTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAI1</accession>
    <name>A0A8S1MAI1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myb-like DNA-binding domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0580113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAI1"/>
    <dbReference type="OMA" id="IYCNEDS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="9"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="9"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="64"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="64"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8077438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="41036" checksum="56CE59F9D8A200BC" modified="2022-10-12" version="1">MYSKYSEIRQSWTYQEDKLLLELIKLNGCQSWNRIANELQYQIKNSIKKRSPAACRERYQNILNPNLNRNIWTDEEEQKLFNLQQSFGNSWAKIASQMNGRTDLLCKNYFYATLRKVLRRLSKAVGYDQSSDLLKQIKPSVLGVIYCNEDSLKTFNIDDEMKIEFKQIIRRFRHIDKSELRMLPEEDIKHIKKMLNKLFTLNNNYISQKGGIILNKNSQQDLTPFDTKKQTDLNLNTQSDSKIYSKIQASNLQQNYKEEYDQQQNYQTQIYSEQQPYQNQKNQSHSNFQSSPSNLISIYTPTPVLTFYFQPNFNIWPIQTVQSYCYPQSLQIPQVYPYVKYEQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ME20</accession>
    <name>A0A8S1ME20_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0530188</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ME20"/>
    <dbReference type="OMA" id="TKQEVAM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15841">
        <property type="entry name" value="SNARE_Qc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="3">
        <location>
            <begin position="130"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8074856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="26053" checksum="BB619B7CDA67B636" modified="2022-10-12" version="1">MNDQFKNDIERIQKLIVSIKSDMNRREQKQKSGQQITMIEGEIRGQLSNLDRELVLMVDVLKLQENNQMEKENEKRKNDIQELKNQRDLIKEQFNKAVQDTKQEVAMQNLKRDDSKLAAMNNQELHKNQKDLQVQQDQLLDRTNDQADQLKQKGKQINLTIDEQNAQLDQLNIDVEKTNKKMMKTNNKLVKLIAKSSNFGLLIVIGIELFVLVYIILWFAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEK9</accession>
    <name>A0A8S1MEK9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0610102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEK9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="88" mass="10846" checksum="E02D790E3080A1B0" modified="2022-10-12" version="1">MFSGLWFQKLENFKKAEEFYLYTFEKRPEDPKILNALFKLNKRCIKNKKKALEYGQKQIKIYQNINLCLVKMLIFEKSWMRQQDIVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MG41</accession>
    <name>A0A8S1MG41_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0610116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MG41"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="101" mass="11799" checksum="242EE828A6EC3934" modified="2022-10-12" version="1">MQQQLTSQVILDRNQEFKGVEFQLNSRKIVIDNIDPLEPLSKYIDADKLNEILNFQIEDSKKGEEGIKLNQHIKYYDFSRPPTGIPKKSFRAYSAIVKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MMP4</accession>
    <name>A0A8S1MMP4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0590137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MMP4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8078045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="104" mass="11930" checksum="4858F19DF48FBCC6" modified="2022-10-12" version="1">MLPRKQLTIQLGSKSRQTSPSSQGSETPKHKKSNSYMQFSPKLDCNNGLSFVNEIKEVKITSPRTEDLHSPKSQPLKQIKYQNILKDEQMKKSQFSKTKSDDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNY0</accession>
    <name>A0A8S1MNY0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0670119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNY0"/>
    <dbReference type="OMA" id="IEYLIIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="396" mass="46915" checksum="EEF62E7387429DAF" modified="2022-10-12" version="1">MQFHLRHSSFIEGFLTQFGPDKQNQVIEYLIIMGIDLANKTGTRNTKELYHLLKQLSKNIKEQTQGDIKNEIKTIQKQLRDLNDLIQISQRENRNVQGTHKKYKQTNESSSNRYEQYENNHIHSHDYQNNHHIHPHDYQNTHQTYNTQRSQSKSPIHFSDRTPHKLKHSRSQSQRNLTPKITESPPPPQIVYINKMHNKFDIKPQFDDRQIKKLMYGDSQNQDRGRYFGKSPSPQQLVYSSQTSGGLSYRQYPGQVIHHQVDKHKQNISPKKLPAYLQNVESKIKPLVDQDKLQYREIQKDRENNNNNNNNNKQDGTIKKVNNNSIQQQNSAETDTFFDIQQYIQDQKKYQFSPVQEMNDDGRNEDDEGSDTFSNFSPPNEEVKEFFQQEYLQQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MRA9</accession>
    <name>A0A8S1MRA9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0660217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MRA9"/>
    <dbReference type="OMA" id="KIPTREW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000028">
        <property type="entry name" value="TBC domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF26">
        <property type="entry name" value="LD44506P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="58"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="355" mass="42310" checksum="559E289AD49DFFDD" modified="2022-10-12" version="1">MLSIQKVNIATLYNRNEEYKSCNRETDREDGSKIQKFKELLHQNIIPINELLHLAWSGVPSELRSTVWRLLLKYQSPNRDANFTIIERKRSVYFGMCDIYFAKNQQYDEREKKILKQISEDVKRTIPDSSVFRNLQIQTLLERILFIWNIRNPACGYVQGMNDIVAPFLVVFLSDYVDIDTTKLQFINEKQLNHLDQRLIRQVEADSYWCLCKLLESVLDNYTNSQPGLIRFYNKFKEILQALDKKLYEHLTTSLSMELYAFIFKWSTCMLLRMFQFEVGLRLFDTLLAEENNYFELCLFIIISILMKFSLKIQKLQYDEIMILLEKIPTREWSESDLSLCLSEAYAYQRIFSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MRL8</accession>
    <name>A0A8S1MRL8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0680176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="GCNVGSR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053329">
        <property type="entry name" value="Merozoite_Surface_Assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39110">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39110:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="133" mass="11882" checksum="0408B312912814C2" modified="2022-10-12" version="1" fragment="single">MNNNIYKYQLEQGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTM6</accession>
    <name>A0A8S1MTM6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0640073</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTM6"/>
    <dbReference type="OMA" id="CSHEHEK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="577"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="485"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8080646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="706" mass="81624" checksum="3D57DF2932A82EB9" modified="2022-10-12" version="1">MVLDMQCNEEEDILAHMAWIHNQSILVYNQNFEITYQNFYLGKLNNLQNKGVNHDYEQIFLESVIEIGSREVNDLFGNSELDLDQEDSLILDSTVHSRYKIKGGLHKIRQMIAALFNNYQKWKFFTFTLYKIKSNELDFRNIHIKVLVTEVKSKLYTLFMFEAMQKVSSKRVLDEPLQFQNVFQTFLSESNNYINVIHLLLLLCSHEHEKNTGKISKEYLKQMRMSTQKFMIFLGTMKDLTLQLTNQLIFRNSTFKLGDILDELQLIYEDCLKIKEISIIPILDSDMIVFNDSDRTKQILKCLFEIAIKYTVTSKIRIDIHQVSPNNYQFQIKDIPFKEENQKSQYQTVLRNTAQLMKTNCKDFDMSNLLELQVSAILAYLLSGSLRKPLELSFDNQFQGTFTFTIESLPINSKHGYHAQQIKPKRAFETSLSMLLAQAQDSSHYKNEESKYMSFTQISKQYSLKPDVPFDLQSAHFSQISKIKQESPQIKPLGSQAQSNSGGTYKVQDSLFPKSLNTNNPSKVIKSDSAVNESSKPMTSALDFGESTNPNIDPPELSPKFLHSVIKFKLTNQCCSKVIIADNDYLNVSVLQILLNKYEVKSDKAFTQQQTLQLIKNKSLNPCRCKNGSYLLYFIDASLPQSAVELIREIKQLFKSFQVDKGFIIAMASYSDMDLKLNCFNSGIDYFITKPFDLFELTAILQYIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MU53</accession>
    <name>A0A8S1MU53_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MU53"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46277:SF3">
        <property type="entry name" value="BINDING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46277">
        <property type="entry name" value="OS03G0850700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="78" mass="9160" checksum="6E97D564CDB9576D" modified="2022-10-12" version="1">MTYVFMKYPERQIRNIVINTDWLAKSIYNIVKKFLPKRTLEKMAFAGKDPKEILEVLSRDIDISVIPKKYGGQNDLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MUD1</accession>
    <name>A0A8S1MUD1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0640234</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CISINAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="245"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="279"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="369"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="403"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="437"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="471"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8080926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="630" mass="74690" checksum="5A69075046F1A0F1" modified="2022-10-12" version="1">MNQKFQQKQDFKCKDHPQTSAIFWCKSIDCRENRIFCINCQKQQKHYQHFIDDVDDINQLNQFLITKSKQSKDLITECQLQYKSTVQSYEQLISGLNYKFCIQEDKINEFQPNQALTALDSLVLFDDMKIHIKSNIIGPLQKIQKILEDLYIQLELQTIKYQISDQQRATNQIEYQKGSFFYIYFFQLSLQIQQINQMMQFKYLINYYQLIPLIQNLSNSLNKLNKQQEAQQIYTNIIKINPQYELSYWKIGDILREQKSYQKAKDYYDKCIQINNNNSASQFGIGECLKETYKYNQALEYYIKAIQLSQSNQLYIQSYGDCLRELERYQEAIQAYDIILNNQSKNADASAGKGQSFNNKLRNMFIVFKHVQRSRGLYLYSQDQYQDAINYFVKALDINPQHIHSLSGKGYCLYQMNDYQQALEWFQKALDINPQNVYSLNGKGNCLYSMNDYQQALEWYQKALDINPQYVYSLNGKGECLRLMNDYQQALEWYQKALDINPQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNQTRLQKLNQFLQNNYFSFFLNQDLMKCLLPTGINISSSKIIYCFYALIRFQNNIPKLNYNLFWVKLQNYAMEQFDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWE9</accession>
    <name>A0A8S1MWE9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="14">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPRIM_AZ9-3.1.T0610150</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21 14">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="14"/>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWE9"/>
    <dbReference type="OMA" id="MHSFWSW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000185">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000190">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 13 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="314"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="882"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="915"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="960"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1004"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1032"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1071"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="17">
        <location>
            <begin position="59"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="16">
        <location>
            <begin position="145"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="18">
        <location>
            <begin position="851"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="643"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="11">
        <location>
            <position position="425"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="525"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="567"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="591"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="629"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="709"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="710"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="711"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="798"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="804"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="825"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="828"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="829"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="829"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8079154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1166" mass="134169" checksum="ADBBEE40FDE76150" modified="2022-10-12" version="1">MTEIKNVQIELHTPHVLNSMEERDSKNLFDYQYDNIEKKGNYAQKYQSKNRKITSNRPDYQLPDNSIQTSKYTLLNFFPKQLFEQFSKLANVYFVLMGALQMVPEVSISSGIPTIYLPLGFIILVSGAKDFYEDYKRRTSDNEENKQQVTAFDGKDFVKIPSYELRVGHIVKVNQDEIISADMILLRSSEKKGICYVETKSLDGETNLKQKNVHVDLLQTFKSDDCFGQLDKRIILKYQAPTPYLYKFIGETTTSNLQVSSINFNNFLLRGCNLRNVKYVFGLVAYTGHDTKIMMNSYKARTKRSKLEVKMQQFILIIFITQFIMCIIASLVYSITYFNNRQYLNYLYIGENTSEYTIPYNFFVRFGNWMLIFNNFVPISLLVTLEMVKFIQGKIMTLDEKLNQPRVQTSNLNEELGQIEHIFSDKTGTLTCNIMEFKQIIIGNQNYGDILRSSDDYIRDDELLNYPQVSNVDFRDKTLMQAINDKNHIMHDKVEECLKMISICHTVISDQKDGKLIYNATSPDELALLNFARFVGFEFLGTDENNIKRISYQEQIIEYQLLEMFEFTSARKRQSILVQVINTGDIYLFSKGADSVLLDQVRLSKEELNKNEYHQLVQRLQEYGKIGLRTLVLSKRKLSKQEYEEWHKRYQLATQQIENREEKMQVLQDELEKNYEILGATAIEDKLQQDVADTIAAVKAAGIKVWVLTGDKIETAINIGYSCSLLTNNLVQHIVDEKEELLINERLDDILNKIPTLQTTQGQALIISGDALLHALKPDIQKKVAEVCGYCEVVLCCRVSPKQKQDVVSLIRGRNQLCSTLAIGDGANDVNMITAAHVGVGIRGVEGQQAARAADYSVQEFRELRRLLFFHGRECYRRNSVLVCYTFYKNILVVLPQFWYGILSMYSAQSLYDTFIYQLFNILYGALPIMIYGIFDEEYDADQLTDNKLQNYYQQGPQGVLFNIQIVLFWVFCGFWQAAILCFFPTYTISENFVENSGFTHHLWAQGTMIFGLIVVICNLKVLLFSNTYTPALLGSISFSMISYLLSWIILDNIPQAEAYVVFQSLFQTPNFHFGNILVIAAICSIDIALNIKLNRVLQKVNQNTSIKTPFSLNNLSIHQPPKIGGKLNNKAHTGFAFNCLDRDELEDQDKIDLYGIDNQVQSQNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0S8</accession>
    <name>A0A8S1N0S8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0760131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0S8"/>
    <dbReference type="OMA" id="LIGVECQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8086348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="207" mass="24394" checksum="B8CE88FADD642B59" modified="2022-10-12" version="1">MGNTCQFSKIDKQHQRIRLIGVECQQFPNKQTKLENYSYNIQDNIDNFEEEFYNKDFEFDIHNEKVYEHEQINKNVEDDKPILLNSLVILEEKQTKTQFGSYNQMASEAFVNCIKQRLSIGNQKFDRFRENVQQQKHLNSIQHLSHTYGRTQFKVTDNDSITQNSSRICKSTRGSKSTFQSSFSLNQKSNIKQISSKQVCNIKIVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2C0</accession>
    <name>A0A8S1N2C0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA repair nuclease/redox regulator APEX1</fullName>
            <shortName evidence="8">APEN</shortName>
            <shortName evidence="8">REF-1</shortName>
            <ecNumber evidence="8">3.1.11.2</ecNumber>
            <ecNumber evidence="8">3.1.21.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">APEX nuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Apurinic-apyrimidinic endonuclease 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Redox factor-1</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName evidence="8">DNA repair nuclease/redox regulator APEX1, mitochondrial</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0780169</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6 8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6 8">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="8"/>
    <dbReference type="EC" id="3.1.21.-" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2C0"/>
    <dbReference type="OMA" id="PMKGPHP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09087">
        <property type="entry name" value="Ape1-like_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000130">
        <property type="entry name" value="DNA-(apurinic or apyrimidinic site) lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00195">
        <property type="entry name" value="exoDNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF6">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00727">
        <property type="entry name" value="AP_NUCLEASE_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 6 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="10">
        <location>
            <begin position="90"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="13"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="7">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="7">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8087377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="348" mass="40163" checksum="41C190B16410D3A4" modified="2022-10-12" version="1">MNQKRGRQAIKRLSPDKKDEVQQKDVVKSKGKQQKEVKKAEDPVEKKGEQKIEKQQIIKGDDKKVKQVLEIEQKFELGDGKFSTPTIKIASWNVNGIRAATKREQAVQYLHDNKFDIICLNELKADQSVFDKENLGSKFGKHYVLYLNFCKTKGGYSGVGIASKVKPISIKCDIGIDKHDKEGRTLTAEYEKFYLVACYVPNAGQKLERLDYRTKEWDVDFQNYLEDLKKKKPTILCGDLNVSHHEIDLANPAGNKKTAGFTQQERDQFTNYLQKGWIDTFRHLHPKEVKYSYFSARNNSRQENKGWRLDYFIINKEVTNAIIVSDINTNIEGSDHVPIECEVDLRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4U7</accession>
    <name>A0A8S1N4U7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0780002</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4U7"/>
    <dbReference type="OMA" id="KNTCEIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="198"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="510"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8087062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="73155" checksum="80EA4AE015FEC2AA" modified="2022-10-12" version="1">MNDSKRVNQQVFRNLSLLGISIFVIINVSNTLVDYFRNAIFALGVLLIIIHKQLKNKKEIFNHYFSNTTKSQSPRQLENQGIMLFDKKNLNCIYMNEYIKKKLNNKSLKVKKVDFSSKQQLDFKFKNIELIELLNQFKLKKIGQMQILDSDLLPDHIIQINQEENNDLINITFYDSKERDDYLKRQYLYQIMKQLFTTISHEFGTLLNFILAIAQVAIDKYPSQQYYYSIKNTCEIMHNFILDMVDYNDILGRQFKLQLEQLDISNILESVTTLFQEQCKQKQLQLEYLNHIPDGQQIISDQRRIKQILIHLISNAQKFTINGSIIISAIQLNEENIEFSVTDTGIGMSKDDEINLREMLKQDFKSEQHISANTAGFGLGCYLANKLCLHLSNQQNGLQFKINEVGTTFWFMVSNNILPTSSLNGLGIQKINKYSYIDLKQTQIEKNVGRRLSRMGSRVSQNSMPKVLSYRGLIDQIKENTESLINEEVNSIERKARTTTLNRNIKQTLKFLIVDDEMINIIGLSLILKRMDIETDYVFNGIECLKKVQENRYAYQGIFMDINMPLMNGYETSRNLISLYGNQIKIIACTAYTDSDTKQRCYDIGMSYFLNKPVNVIELQKILIQFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N960</accession>
    <name>A0A8S1N960_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0790105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N960"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13905">
        <property type="entry name" value="Thioredoxin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin-like fold" evidence="1">
        <location>
            <begin position="31"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="599" mass="71036" checksum="EC4274E66E86CD39" modified="2022-10-12" version="1">MDIDGLENLSFSDNYDCFQTNSTFKVEEGIVYLLDIWATWCGPCQIPMNHNQQMLQQNEHWKNKVKIIAISLDEDSQNVIERVKQKQWGLIEHYRGDYKIQILKLKQINQFKLNKILILNKIILIFKKQHNHQKKKRGFQIIKKQNKNHLGTTRNIIILKLLNYWNQDGIKKTHQRSKLSLVYDILKEDKESLQQFLDTYWSNIPENLKEVTFSRIRDPQTISQEIKQLFQDIYLGFGVKLEYNNSKEISFNYSLQLDPPFAVKRRNDFWIQQQNLNEDILSKAKKQLLKYEALYINYPEAHQCFQYIFNELIICLNKQEIFVYKQLKITDIEQSNEIEQIINILQKEHILQLLSKIDKQKVIEINIDQTIQQQQNNILVHNPKLNYFIRDSQQEVFQQILNEIFEIIPQDKWIITKTVSKTISVQYPGKYCIVCNKDISNEYQQYYCPFKNEHVCMICAEFDDKCKVGMERFKYHDTLMFINGPLNDLSVLSDIDEHKIGRNVQLKEGEKGNQNYSQIMTCSGCYEGSIGFRYIAANVKPGKYQMDGYVEYCSKCFEILKMKGSKKAKELIDSDIQFGMNSDTLFTRILFNYGSYQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9B9</accession>
    <name>A0A8S1N9B9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0820027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9B9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035908733" evidence="2">
        <location>
            <begin position="23"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="46"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="273"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="384" mass="44662" checksum="8C6D7EFAFD76D0CA" modified="2022-10-12" version="1">MKILVLCLIALAIADKYDQFRAFDEDEFGRTLIDTLQMQMSTGEPIARFIEIMRNLEISIENEQKEDDKANNEYQNQCTEDIKVLQQESANLERRTVEIQSILDELEPLRSYKQGQADAKNAWKIETEKKLADLVKKRETEKAEFDKKVEEHDYATFVIETVRRMFSDKSQSFLQLNNESQWQKVRDYFINASEQAKKFEIKKSYSKIFNVLAEIANSAQSEDFQNSPTVNRIVNLCDLMLKQIEDSKALETKAEQKRLNMFMLEKGNFDKDLTSLNNALAQLNAAILGLDNRIQDQKRDLTDYNTRLSAKNKQSEDRGGECREKAYNYQLTREKREQKRQLVSQIIGAFSANQRDFSEYVKLRGQAGDFKGKNFQILGNPTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBF0</accession>
    <name>A0A8S1NBF0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myb-like DNA-binding domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0810182</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBF0"/>
    <dbReference type="OMA" id="VIFNHKN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="83"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="147"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="154"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="210"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="525" mass="62799" checksum="7332611709BFB622" modified="2022-10-12" version="1">MIAQKNTKIQNDHILIGSCEMEDLTIYPYELVNREFFGFIEKTSFIKENQELLSKVGQNLRLNYKDWPTVDQAILLFQKNIKNAKKKSIKWSDFEKKLFYWIVMRYYIYKGIKDNKQLATEEWREISKMVLGRNAHQCRLKWEQKYKIPLSEAPWTEQEDNLLYQVHEEFKKTGRENKWSQISREIFKRSNNKIFRQPKQCRERWINRLDPNISNDPWNKQQEIDLLKTILMRGKKWSELSTLYGRVRTENSLKNKYNSILKKEKMKYEFETINPHLFEKVQKLRKDYSKRYGNVTPIEEIDNYEWQFIVLAIQGLYIEQCMLEGKIEEAEKINNDDFFNLFHEDPSIKTSKSILYKKELNIKKVDQNLILNNEKCGVVIFNHKNNKLYLTPYNVIDFQNIVLNQIKKKVKVEDNTTTSSTQSDPNQNYIRNSIGTQSIQQLLYFANQPNYFVPINQSIFNSYWLPPVFPQQIVSSQVQNNNFIGLQKFQEDKINENENQKKGDLNDIFEQIGVDIKIVNTDDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-06-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDK1</accession>
    <name>A0A8S1NDK1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PSI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0730012</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8084834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035756887" description="PSI domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="2242"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="129"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="792"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="1316"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="2106"/>
            <end position="2153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8084834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2242" mass="246784" checksum="DE88E94F497862A3" modified="2022-10-12" version="1">MKRTLFVIVLINIATCQVVSKTEACTCAQLLIASDCNRNYQCSWNLTKLVCEVSQSTGTEITIKNYGKSLYCEGQGQNDCLNKNECAWIDNKCTFFTSCTPFQKTNKDECQAQSKRCTSDGYTCVEIDLCSTYVTSNSCYQSRTNYCFWDEITKKCSDVTECSQLPSTLTKDSECRSQLKYECTAKTGGGCLESGTNCEDQISEVGCVTNKARTVNCFWGNNKCYEKKCEFALTSNRTHDDCQKFSNKCTAKENGGCVDIKTCSDGKISEGCKIDSTGKDCYWSSIENKCKDKLCINAPNTLKSNSECQKQLLPSCITNGAGCVDDISCASSIVPDQCSIDRFNNKQCTWNGTCKDKTCENAGSEIIGHEQCSTYSIKCTGKANNIAGCQNRTCENAPNTITTNSGCEEYLSNKNCITKKDGGCVTNTICSAIQIRDACFKDINGKDCYWDILGDKCLDKTCASLPSRFNTHSLCNEQINTCTVNSSGTCVDLICENIIDKDNCIKNKDGGECIYYEFCYQKQCNSAPQTYITHAECQEYLKNCTLSNTKKGCMDIPLTCSALTIKENCELKSNGGRCGWNGLSCIDFTCSTAPIKTDDVYTVALCEAYKQKSNCVPNLSKKGCMELVIQCELRKIVEQRNVVGTKRNGGKTCLWDGQNCYEKTCANATRVGYANSVVVLDDKVSHNDCQNWLKDKDDNSLCIVGKSNKVCAVYPTNCDGLGENSCLPNVIKIPNTDPIQYLNCYWNTIQQLCVDGDVCANLDKSTHDGCSNASNNKCTISADGTKCVDRQVCNQYSKKIQCVKDKYDFPCKWDGNDNDTNKCINQECQDLSLSQCEIKKNTCAEYKYREACLGGILGDKQQCKWNEATKSCVNASVNCSEILTFEACKATIVDGVQQCVLRPNPFLQDLVCEDAIFDCTKVKGSGLTVHYCNSLNKRCVVNKTGDSCILGKEYCAEYTSHSECAWSLYQPFQSIDWPFQRCYMPDNICEEMDQSCINIKGRDHNECQKHLSKCSWHFKCIPNQGLFQGTYTYDMCQNIDQELSVKNDGSGCYFVAGSPCSILLPTMCTRGIDGPCYAFNSICVKGPVVHCGQVTNQANGTGKITPQYCFDVSNGKCSANITQSKCRQASEHCSDYNTNNQNLDECYFMQSTQGMWCILNATKDTCIRYNEQQHKCSDIILGGQINQTHEMCQLYNVNCTATNPPSNSCTDYLICKNYKGIYTFFNCFQFDIKCSVNFDNTACIEKQDYCKDYSLKENCAYAYIEGPCVWDEIQKCIQKSCSWITDLTSPSAEQCQAKQLSCNLPAVKEICAKRGPCSSYTESTSCIVSNIGQKCIWNSISKLCFDRTCANADSTYVNHIKCQEVGNCTVKVGTNGTTLEGCINLDACINYKVEEQCKINAQKKECVWSLAETPNKCVDKSCRSADPNQYTNFEQCQNYYKIGECTLGAKKEDDGTYKEFGCMPIGLCTEYYTSGQCKISKNKDDDDNFVNCYWNETTKKCGDASCAQADDTFTTHDACVSYGNAANIKCTIDKDEKGCVPIPNTCEEMSKVQCVDEDAKQNKCIWLESNGSGQCVQRTCQNAVNPTSSSDCSSHLYYCTMIDTENKCKTKSCEDYYYTEDSACAVLFKNSKCTTNGKFCVNRGSCTIVQNQNGCTSDSSGNLCEWIVPEDPNKIAYCVLKTCNTAPKTLVSESQCLQYFTPKSGVTCNTQQGGGCIERSTCSAAKGEAACNTDANGLICAQDSETFICRNQECKDISGSTHQTCQNPSDKNLKGKCTAGKSSRCAPIQNCTSTTVQAACVVGTDGPCIWLPDYPNDDNTKGACFRYDSCKSLYWTQDSLCKYISDKCTTDGTSCIGITSCDKTNVNGGCVTGTDGTCITLVQSLGSANKSCTKYVSCTLALFKTHSECQAANPNCTTNGTTSCIELGQCAIYVKDACHFNKVGRVTNSMKQITSTGKCKWDQTINSCRDENCIDFQGSTHQACSSQLITCTTDGTKCIVKDSCTQYTKNNTCTNALGTEGLCTGVQGTNTCRVRTCDDITGGTTLMACSIVNNCITDGTKCIPRSTCDKYTTKIGCYSKGTDGFCIWTETKIGNTTTGKCSLMRNCASATKDQDACQQASDRCKWTAATANTSSSCVDHTCESFGAQSGRCLYFPNWDSSKLNICRNVNGKCTATDPKALLDTECYNLSAYMYSWNSKTNSCTQCQIVMTPNNSTIGNDLNQTDDSGYVLSLTVLIGYLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NE59</accession>
    <name>A0A8S1NE59_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0870003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8090890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NE59"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8090890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="189" mass="21967" checksum="B4A47DF07A38FDFE" modified="2022-10-12" version="1">MNSKASQIRSIQRALKFRDHNVPFWTVLDVLEDQQRTSVRVQTYMQSLKDVQNYLIENQDRQSEVFANVGLLKEIQQILIVDMSLIQIQMTIGFEQCYFDSLVIDIHELLVGCQQEQQNAITRRIGQSKSFSEKQKILIVGINLSAQKDAKSSSQEPFQIVMINGQIQSVSHLIEIHKLRQLRLIYLFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NG43</accession>
    <name>A0A8S1NG43_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0810076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NG43"/>
    <dbReference type="OMA" id="TYCCKEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="404" mass="48234" checksum="D19F64D279E9EE33" modified="2022-10-12" version="1">MNELIYNKRENFRVEIRRQSLHNLFCQKRIIKQEKKKLNCQLDYEKEQKNNEQNSIEKCKQKDYDETIQEYLFQIFNQDNISIAFSIDLINKVLNNQDLREFFEDNKRYKIQLQSFLVKVQNKFSKSLEKQKYDQLLLTIIESDLLKLSDLKQSFTYCCKEFITNGITIDTSLFMSKMMKKFQNASEYFWKELNEQHIENQCAWLIILKFLRENQQEYAISKLLISMILLLMQIAKPKIYQGIVNNELILTLRQNLYTNETQAIKSLECFNYIFQQGEKEKRFLPLIINAFLDLLLNNPHASYLISISILDCLEIVYSDQNFGSSLLNNQELMNCLISMLNDEIMQVNEVVLKFLLFYLEQCSDDFIMNIISDCNFQNVVEDLTISLNKNVQKYAKKMKLIYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NI68</accession>
    <name>A0A8S1NI68_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0900197</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NI68"/>
    <dbReference type="OMA" id="YKTPIQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040626">
        <property type="entry name" value="Pepdidase_M14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF11">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18027">
        <property type="entry name" value="Pepdidase_M14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M14" evidence="5">
        <location>
            <begin position="257"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="544"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="557"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8092458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="720" mass="84909" checksum="B6827006B7EEB017" modified="2022-10-12" version="1">MKNISQTVDVKNFSKKEDKENLVFYQSIKDKLPKPNPILIQQLQLNKVNSLLPLKKNYLEANEQQITYKTPIQQQEQECLTQKPTTLQAFLKPLKLVYQKYQPQQNQNLYATLIHQPGNNFDASHYYQSDEDIKFDSLFESGNLFQVFKKSDRDYILLLQNDINTKGYTQWFYFSISNKNSTLSNIKLSIININKDMCFYRQGMKILINECNCWRKDSLGLSFKKNNIQRNNSSFYYSLSFNYTFIEQGTVYFASNYPYTYSNLQTFISSKYLVYDKILKVKNIITSQAGNEVQIITITNDNKDQKQGLLFIGRQHPGETPSSFVIEGIINTLLSAEADELRNKFVIKIIPMLNVDGVVHGNQRCGLGGFDLNRKWGCNRDDTLNEIEKLITNFNENYPIQLILDIHGHSKKLSAFFYGHSCNEFITELCNSDKRFSLENSRFMKNTKYLNHTARVFLQKLLNMNQNIYTLEISYFGYKDQNQIVDFTLNDLRSMGREIINTLSKNIETQIWTESILINEQNSDISFSDSSISEDEIEYEEIKQIQPEPLREKNNNLERQYPSSNSFKQRQLKSSQSRNRAKSQSIQNQMKYQQFTYNFIDNSFNNNCNVKNDNYDHQSLFIPQQKPKQSEYTIQPLVIMPQHSLKHQFLWQNRLKQQNNKEIKFQNLKEQRDLKIYQEFKDYVNNKQYSNKPLLPKYSFNDTDLFKKSQAKKQYLIINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NMD2</accession>
    <name>A0A8S1NMD2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0920192</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NMD2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8093240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="125" mass="14197" checksum="F2D77519ADD93283" modified="2022-10-12" version="1">MLIQLKSYNQITSSGNYIIKPFIIKSYIPKHSLIVVSPINHPSSQLILENSIKLKSCKTNSQLVHPNELQESHPIKHSLIDNQNSVIPQITFVAKRATVTDEIECQMVIEIGKIRQLQYQHVPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPJ0</accession>
    <name>A0A8S1NPJ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MaoC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0880097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPJ0"/>
    <dbReference type="OMA" id="GCVFLHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03449">
        <property type="entry name" value="R_hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050965">
        <property type="entry name" value="UPF0336/Enoyl-CoA_hydratase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43437:SF3">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43437">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="20"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="141" mass="16244" checksum="7C4374F3D5925732" modified="2022-10-12" version="1">MKLRRIQYFFSDFRIGQLFKLERIFTEEDVKIYANLIKDFNPIHIDEYAASQSIFKHRVVHGMLSSSLFSTLVGTNFPGAIYLDQSIFFKAPIFIDEQVIAQVLIQDIKLKKGRSILKLNTTISKMDQKIAVTGEAKILIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPQ6</accession>
    <name>A0A8S1NPQ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0820007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPQ6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="122" mass="14298" checksum="B9003A6C73C4A35D" modified="2022-10-12" version="1">MKISMCFGSGVVTGRIILLKVINYSHHQNSSHYLEHPTVLKSSFVSFYKNLSQFIYFIIIRRKEYSVIEEIFIQIKIIIQRGLGVYGSRTTPQLRNIVQPNSQFYILRMKVLRQLTSQKIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTM1</accession>
    <name>A0A8S1NTM1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0930172</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTM1"/>
    <dbReference type="OMA" id="VMVELMT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF106">
        <property type="entry name" value="NIMA-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="24"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8093581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="417" mass="48360" checksum="F4BD6C77BB9E32A2" modified="2022-10-12" version="1">MGAVQAYVNYNTEMTLTSDLNAQIQCCGTISHPAFGNIQLWKMKNNTTLEIFSLTRHIYYQDSILLTIHTQRTQLKHPNLLQYYACTQNSPSFCGNVETQQFYFEYISQTLSQYLCGRSEPFKEIEVWKSLEQLINVLKYLQQNGYSHGKITSDNIFITQDQTLKTLDQITIKSTSQQIKQDVYDLAQVMVELMTKKKFHLSLKDTVISLIDEYSQQLLQLLARMLNNTPEKRPDFIQLSEIMLKRQYKSTNIEQDIQLSPQLIKFQQSKQIENQRINRLFNQNIQSRSPSPQKILHYSPQRLSPYKISPQRVRILSPIKKEPFYSSINQSFQQLSGIYRTESPIKGNITGSQASTIGSYGVSVIDTYNTKYAFYTQRGCRPNKILIPQHKSDVKRNLFKEDLNQTSSSQPPIEIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NX04</accession>
    <name>A0A8S1NX04_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1000088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NX04"/>
    <dbReference type="OMA" id="DWRMCKD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="65" mass="7862" checksum="38336A81CF19FA69" modified="2022-10-12" version="1">MCDAINEKDERYKYASELMDKDGCKQVNLELTQCLKQYKKDWRMCKDQTTNLQKCLIEQKNQRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYK9</accession>
    <name>A0A8S1NYK9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0970036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYK9"/>
    <dbReference type="OMA" id="AWEHEEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070224">
        <property type="term" value="F:sulfide:quinone oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070221">
        <property type="term" value="P:sulfide oxidation, using sulfide:quinone oxidoreductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000593">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015904">
        <property type="entry name" value="Sulphide_quinone_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632:SF2">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="54925" checksum="E3D30ACF9A74592C" modified="2022-10-12" version="1">MLISGMQLRHSLTPRFFAGVFNKRKPTKKDLDHYDIVVIGCNLGGVLSRQLDKVSHGKYKIMVVLDQNINQITPIRQIYEQQKTAKTDFLLNAKLSLNMYTAHSDQVGCQTILPEENAIVLRNGRRIGYNQLVVAMGQQVNYNAIKGFEDAWQDFDSPVFTNLDHPSWRSSNHKYTRWHYNFNHGEAYFCIPQFPFSGEVESYNFFLSQRIWEWQTANGRQSPIKKFTIIQPNERFVQYNDAGDSFFKEELKRRNIYVEYGLKLVEVNKQYNTATFEDVKTGNRQTRNFNHLYAVAPTKAHEPLVKAGLTTSKGLLDVNIKTLQHNKYKNIFGLGDVNDLPTTNCFWAGFHQLHVVRNNIERNIAGKSLNAEYDGYSKVPIILGQNTLTFLCHKYNNENAWHNLYFSNGGFLAALRYYNWCKNFKKAFIDIYLEKNWGPPYYKIKKSFKLPEGENDDHGFLSKLLPGKKESHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P0T4</accession>
    <name>A0A8S1P0T4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1030042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P0T4"/>
    <dbReference type="OMA" id="ENIFYGI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048211">
        <property type="term" value="P:Golgi vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024095">
        <property type="entry name" value="Vesicle_P115"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013:SF0">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="723"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="85"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="171"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="354"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="725"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="771"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="784"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8097081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="799" mass="94572" checksum="44213427327DA65F" modified="2022-10-12" version="1">MSDEEPFKDDNQDQGDNPDDDNQDYPQDQPDDQDNYEDGDPFEDGDNPKDDDDIKNNEISNDLKLTIDEQGLDDNQKLRKWKDLLSEHVQKVQKLRMRNNNLRGQLKKLGDKAEKLVQSNVRKQKLLEQKLDPKHYNLAEKKILYEIKNGKSKQDFANKIDPRILDLLINIDKMEQSNTSMREQIKALKDRNKNLEQTEEVNKLSNEIILIEKQIGDEDRLKKKMQLIMKEQEAELKQQQFDCDYESRLNSLKEQIKKLKDNIKEAQKVELHETRKEVELNNYFAELQKIYRQMCNQLNKPCEEKQHFKQEKQKEQDNTNEHLKKAQEFAQKSQEENLKRYLKIKENQQQPEETDELNKALKEFKENNDKFKQLQEEVKILQEELDKKEKDQNKEKKRLIEEQAKLSEDVLQLAKQLKEKEVQTKSSVTKLNDLQRQVQLLDKKQVQEPIQSTLQKPECIMFGIDKKTETCLPFDDSKNLDWTSNMPINQIVVCKNSQNNLIIGLELTYGDAENKEDKKTTKHIGLQEGDIGKEQINITSEEKLSYISGFHNSEQIIFLKFETNKKRVIQVGNMKYKDNYTKDFRIEIKDREILGFNGILDYTSESQNPSERYLVAIGLNLKHKELDISTKKRLKKQKMLEEQEKQRVPVDYRKITYPFKKNHPKHLELIKSQPKLDVSIYEEEKAKLFQKEKERFLQAQRAQLGLLSAKYFSPSTYKQQEYLLEDQKRKEKSDQRKEWVEQKREENKNNLTLPIIETKTPADQDKKGAKAQKTASPQKQQKQAQKEVPKKDDSKAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P195</accession>
    <name>A0A8S1P195_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T1010090</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P195"/>
    <dbReference type="OMA" id="NRIDIWS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001381">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="46"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="166"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="9">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8096433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="289" mass="34227" checksum="FB4112D84BF0E7E3" modified="2022-10-12" version="1">MFNSAQKRAPPQLYVTPRYRTTQSEYNNPHSERLFEKSKENSFNQLKILQQIGYGKFSKVMLAMLNGEKFALKIINKSCTIKTQTQQHLQREIQIMKQLNHPNIVKLFKDFSDEKNVYLLLEYCNQGSLFNQKFDQTQIKQIIKEVLLALKYLHNQGIMHRDIKPENIYLHNNHVKLGDFGIAQYIKKQSKSFCGTLDYMAPEVINQNFYDNRIDIWSVGILAYELSNLEPPIKDRDQKLQMRRIISEEVQYPRHFDLKLKNFISKCLKKKCDERATIDELLNDEFIHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P3K4</accession>
    <name>A0A8S1P3K4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1040124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P3K4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="50" mass="5974" checksum="AD347046343B4CB0" modified="2022-10-12" version="1">MQIQNQQQQVLREFIITSFASFEKYQSDRIQRIGLQLRSGEVPLLKNMMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P536</accession>
    <name>A0A8S1P536_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1060054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P536"/>
    <dbReference type="OMA" id="KGSTQMA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="493" mass="58425" checksum="D8E5F964193A28D8" modified="2022-10-12" version="1">MHKLKLQFLNQDVEHNYIQQHQFPKRKFYLKVCTVGFQILLIFKLIVSLIEENYSVTYPLLITWGITISFILIPKKTDIQIRITIICLNLIYVVYLLFFDPQEEAQIMYFKGSTQMAANIVNIFVLEFIDSSILNVVILTLRIIHLNYYSLKTDLPSIAFGIGVHIILILMIYVYHKALRSQYLLTKTDQRWENILKQIVHDQKFILINFDQEKIKFISITSTFSKKIQNQDEVINFIRNSKIQQNTFEQYLYKNMNKFQQNCIDSLNNTIMVTYERQLLKLNFSIFFGNQPTILIQKYSPQQVQNMKNYQNISKLYLNIIISLIKIIKERKKFDLFKFQLIAKKLFLKNLISTIQNQNFSSYIISLKRLLSKISLLSNKKQQIQLLCQDVQFLTIPKIFQLFLVLLVECSHNEIILIQHIRIEKEVITIKFCGAFNIQKIKAYVTKLEYYFLLIFQNIKYDDDSVTVKLNQEIFVPFKNLSSINTSEDILLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PF20</accession>
    <name>A0A8S1PF20_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4200 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1160112</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PF20"/>
    <dbReference type="OMA" id="IANNDMP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051147">
        <property type="entry name" value="CFAP_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025252">
        <property type="entry name" value="DUF4200"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683:SF3">
        <property type="entry name" value="CILIA AND FLAGELLA ASSOCIATED PROTEIN 100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 42 LIKE-2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13863">
        <property type="entry name" value="DUF4200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4200" evidence="4">
        <location>
            <begin position="115"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="132"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="305"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8101746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="510" mass="61413" checksum="8FAA133053632CC8" modified="2022-10-12" version="1">MSEKLPQIHKVNKSDIQSPFRYPSDEEVFLFREQDRKKQEAQNHDEMRIWDKKTATNRTQLKHFKSYETQIDDKFKSKTAYNDKDKKIINEALQIIQERKKQRAKGVSKEPIVTLLEQKKEMFLVSKAHGIIEEEIDKLKLMSKEKKEALEQSLKMLETDNENFQKYLDTNKQQKLDAEQKADQEVKEKKAKESEIKSINIKITSLRAEKTRNEEAVNNYMDHKKFLDKLAPKEWHDAKEKKRQQLVNRLKEELIKSKGLTEKQFQQMVDNKKLDELEEFEDEYEMYFKQPSQLVEIFNDLEERNLFLIQTTQDAEQNLEELKTKFNKIKKSLDDKVFTLQQNKEQLKKNYENVCQQIKTLQLKGTEKNAKKDHKELRKQIIEVCENFPNEIQGVHDLQNKETLDILVQIELHLERQLKQIRSFRPDRVENFRQECERDWKVKQRDSNNLKLKQEEERRIKANQEKIMQPSKKKTGRQPMFRSKPFDRVIVQQEESVNNEELEDQKYFME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PID3</accession>
    <name>A0A8S1PID3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CSC1/OSCA1-like cytosolic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1190170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PID3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF83">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="377"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="504"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="570"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="647"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="2">
        <location>
            <begin position="185"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8102945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="732" mass="85446" checksum="6B4865EDA22F6A76" modified="2022-10-12" version="1">MDYNPLQMPCDWNVARKHALARRTAPDTKARLDDDDDEEPKKPETCPCCGFEIERKEIPYCDDIKSLKFLGAGFPLFYNFLKFCILLLCLQSLVALFNILSNYNGEFCQQKTLNPISLQMEPNCQESMFLKLSIANKLNNSEVVVFIQKANLIMLIIMIILLQIFRRHQKKLDNQIDESQLTPSDYTIIVTNIPKTLNVNYRWELTNLFQNYAVSDNNFQITVTKVVLIYDITEILVEEAKIQKTLQKKKIALQTSNMKYDCQDVRDSEVEIEISQKRIKDLQEEYFWTNRQFSGIAFVSFESEKMKDLVLSQNTHTLYDKIKTFLYSGKTPGLDEMELQWQAQKLFIEQAPEPNDILWENLATLTQDKIVARIKGFFINIIVQGITFFIIYYLSIRCIRLVYNEELEKRKIGVDDKEKLKNVQMISFAIASTIVLINKLFIEPLMKWITKIERISTNTKFQISYANKLTISLFVNAAIVSYVIDILIFSNVYGFGGFIYNETLIFILNAAIAPLIWLIDPWTLIRKLQRDHQAQKVNDCLLTQKEANEIMEEVDYQLAMRYADIIKTMWFTFFFGTAIPLGVFSSLIGLSLFYLVDKYNILRRRTVKESISQELSWQMINMLEFVLLFNPLGNTAVSLFLNQNFDIYSTLGVIIGLSFQILPIHRFVDSMFPIKNFEEPVSYKKAQIEFDTDYDRENPVTKQKAIAEYSLQLQGITQERKVEYQIMHEDHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIK8</accession>
    <name>A0A8S1PIK8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1190123</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIK8"/>
    <dbReference type="OMA" id="QGFTMEK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="39"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8102864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="401" mass="47124" checksum="2D8C17EB574D9168" modified="2022-10-12" version="1">MKKNNKKSQTTKIKVTPCSNDRYYMDQLIDEDVYLKQNYKVVRELGRGGYGVVYKALGLNQGQIEKTKKYAIKVNFSTVSPELIFAEIGFLKLVYGKENMPQLVNLFLKDQKIYIVIEYFTYKPFITFFANFDMMQIRKYLYELLKALNILKQNGIYHRDVKPGNFLYNPKTCKGILIDYGLSEIDRSFVAKLIEKEKDLSKNNPQSDEVQEIRRKINLYNEIQKTIDQIGQNKIGTESFMPLESILHHHDQSYEVDMWAVGVIFLQFLTKKYNLFSNVRMINKPVGPKNFFYVNFILELASLFGSEQVRQICDLFEYDLKLPSVSATKPIKWKQIIHMEGFDDNAEDLLQKLLCLHPKQRIKVEDALKHPFFQTLISQELQAETNFQLEQEFNMIKQIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJD4</accession>
    <name>A0A8S1PJD4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1190148</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJD4"/>
    <dbReference type="OMA" id="LCNYNSL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8102903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="321" mass="36853" checksum="39C7935B181A5373" modified="2022-10-12" version="1">MGDKKKKGDQVEDFSTEQLNKLYRKRCEINGVPLCKIFKERLEAVCSEGEHLQNVKLWEEMGPVGVRAMMESFTEIGYKHLKQLRLWRVKCQDEGVRTICLYIDKVRLLEVLDLLDNQIGVLGCKFLSDILHPKCESKLVKLKLDHNQIGSEGLAELTKGLAMNNTLESLALNYCGIEADGARYLQDILANVNSKLYKLKLSGNRLKNEGAYELFRALEINNTLERIKLADNQFHSDPSDQTLINKILSTFELNKTLGYYDLKFNLLSDQDATKIIPLIEKNKSIFFIEISDQISKPLTDKLKSLTKKRKPKKKKKSKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PK04</accession>
    <name>A0A8S1PK04_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 11">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="1 11">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T1210064</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="1 11"/>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PK04"/>
    <dbReference type="OMA" id="INEEDCT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07852">
        <property type="entry name" value="STKc_MAPK15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000238">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000166">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="13"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="378"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8103470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="486" mass="56162" checksum="C69FBEA733060C50" modified="2022-10-12" version="1">MAEEIEPHIARKFEIIQKLGKGAYGIVWKAVDKKLKTVVALKKVFDAFHNATDAQRTFREIMFLQELNGHENIVRLLNIIKAENNKDIYLVFDYMETDLHAVIRAGILEEVHKKFIIYQILKSLKYIHSGELIHRDLKPSNILLNSECHMKLADFGLARSIAINEEDCTPPILTEYVATRWYRAPEILLGSTNYTKAVDMWSIGCILGELVIGKAIFPGTSTANQIERILELNGKPTDQDVESLESPLAAHILSSVTVTKKKSFQQFFQGASDDCLDLLRRLLVFNPKMRLTVDQAIRHKYVKEFSSPEEEIVCQEPIKIPMNDNKKFSIKEYREALYNDINRRKKEQRKKWQAKYLQQLGMNPDELIDGNQYINQSDQQQQSGSIFQKQTKMENHNSSQVVQKSNIEEIQQIIRQQQIQSQQLKKSQSQATVQPAQKLTSQKSASNIVAQIYNNFQGQDTKFHQQQQFLQQQLQQLQQKSLKKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKV0</accession>
    <name>A0A8S1PKV0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1200074</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKV0"/>
    <dbReference type="OMA" id="NPWESEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052438">
        <property type="entry name" value="Chromatin_remod/trans_coact"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572">
        <property type="entry name" value="GLIOMA TUMOR SUPPRESSOR CANDIDATE REGION GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572:SF0">
        <property type="entry name" value="GLUTAMINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="418"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="452"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8103178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1186" mass="138288" checksum="9B2B49BA0BB46B6E" modified="2022-10-12" version="1">MYSKFIQQSQSQKISEPATRIFRFEKQSSLRQKLTSNETDNDAISRRDRAIKSVTIIQEIIVSSTNSNNPKKLLKLISQFMSQEQNIQQITEQLDKQQIVTLFLNIHLFQIPQLVHFLHLLYDCLIQLDTQYQDVFKYILLLNYELYQGQAGQLTRILEILSEMQMLNPDFSAFENEFQFDIEEISNSFNRVQLRILFLSSSIDILHSLKGSVALFEQLETFVFSSIKKNEIPNFNDLQTLVIAYILLSEQLDISNKLGYAKQALEIIGKSPDYSIFELKKRFIMNATKLSQKYLGQQIYDQLLSINRSFSPGFTRQSITHLNQKDNQFFKFLVSQLKITYAIDNDSNFQIDSKCNSLLTEPMINQLLDRVIPQSIKKEKVNSTFTEQSQQDKKPIIIKKQFSIKQLKQENISSSQIQVQNQVSTPSQKNQAQQSTSQYKQNTTNKLDSNEFEILQNKLVHQQQELQELKQLYANQMLQKKQDQSVQQNQQNEQLKKKIESLEGNLVQLKNENTIYKKEKDQKQNEVHELRSFIQDLQDKQQKLEKLIQQQQQQQQQQQQQQQQQQQQTLLQQQLANPALQYLKTQQSSYSTKANTNMNIQIPISNSETKNRQLIRGQSQLSHSPGEEFKNEMDDTSPRIIRQNQTYLLQLLDLLDMNTGFTKIYNKLHSTNDNNPWESEVTVIHNFKVDAMITDSNEGKVLMLKAFNSQNNQICSEQIPFDLLKSLLGYVDFQDSLPTNLPNISTTHQFFKFLILPYTAVVQDDNTQQYKIQLWPKPYGILNGLNLKLDFLDFNCLVYVHHLETDQFRIIIFDPANYDCFKLDLELDYSTMDIFFQDAKSIKDEFNYYCKSKLLRLTEKSPKFGEDDVAEALVERHFSKEKVKAVIAEAKPTQSFSQEQITLKSPLEFLKYIKSIVQAFEQQLKALQITFANSILGMKYFRCKTWNAGTKSQIQIIKEQIDQQQLSIHLVNCFESFGPNKTKLKVKGNTLIHFSAIQREFAVSYDKLQNEERATILQTILYSYNLNIFEKQCEQDEQQFDKNPIIQSIYDCGSYKKVIAFDNGKISQVTIQVIGSNRRMCGIKFSVFNIDETIENGVFLPVSQIEWDTRAMEKQKIKASVQNVPFAEYLLTQILKCPTTSTILFKKILNSDFKSNQINSNEIKNRKTFIERIDNILTWQEVIANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQM5</accession>
    <name>A0A8S1PQM5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1280090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQM5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006742">
        <property type="term" value="P:NADP+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050241">
        <property type="entry name" value="NAD-cap_RNA_hydrolase_NudC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904:SF1">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE-LINKED MOIETY X MOTIF 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904">
        <property type="entry name" value="NUDIX HYDROLASE, NUDC SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="12"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8105750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="133" mass="15474" checksum="412C129A742671D7" modified="2022-10-12" version="1">MVERLQDLESECLREVQEETGINVLPILNKMELKVLYESVYPTQLQVGQFPQKQTLCIFYEVKLNESCNNIKVKIQESEVDDFKWIPKNQLLDIMNVSTNDQQYKEMSGIYPNQYGSGIGEGHIKAFMNSYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSF8</accession>
    <name>A0A8S1PSF8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1280021</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSF8"/>
    <dbReference type="OMA" id="TIMELKP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000334">
        <property type="entry name" value="Heat shock factor protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="3">
        <location>
            <begin position="9"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8105613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="267" mass="31481" checksum="BE9924FF72B4A80E" modified="2022-10-12" version="1">MSKSRSQSSIPAFLQKTYDILENSQLLDVVGWNEDGSGFLVKNVIAFQDQVLPMYFKHRNFASFVRQMNMYGFHKSRSDQKENEFIHPHFKKDQRNLLKKIKRKSGEHGDDQFTIMELKPHRNQNISDKQIQQIMTKQQELEKVCKILIEQNNKILQCNQQLRNQLVQEKFNGNKKLQKLKDYFICQQQIQGDCLQKRSLEDLISSDNEELILPNIKKIKGEDSDSTFDKMEQLYGQPPLMLTTTLVENDGMIQLLDDHQFNDIYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSY5</accession>
    <name>A0A8S1PSY5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1310020</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSY5"/>
    <dbReference type="OMA" id="SRIWMEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="83"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="240"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8106500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="532" mass="63163" checksum="CE5F48D95E7EC4D3" modified="2022-10-12" version="1">MESKSMTISTKIQQSNYSALSNLPRHIINQIKSEGELYITAPNKCSKIWIEMRKNDFVTDAQLNFTIVQRIFEACRSEIVQLLKLQNEKDFFELFDQDNDGILNEDEQILVFSVIKEKMHQVATALLKIQEYILFKQLMKELRILEANIADYQNQLRQRISNKERKVYKEIGQEKLDDFYDKYYQEFQQLTKYKVDRRAQLKISQEKELGILEDRLSKDTELMKVKPKKKLKDLQTQEKLVSLEERVEEALDFRKELKDLEKHEQDRVYKVQKYRIEKQHSDLLFKQQKEREQLEGKLQETEYKLIIQMKKDYDVLLKQINLHNNEITRIQSLASNQAMKKGLYEGEAKRQKVQSQLQNAIIAQTKAISQPESKINDLDQFHESETIRKLETRKSIQFVGSPGKPVYSEESEKTPMGSTFNGKSKNFYQIRKIIKESKNITQFYIKKNYGADLPVNFIRSAHNIQGDQHEKIERFLSVKRKSQHELLPPITQLYDDDMQEKQIKAITQTDQEIKREKALKRAFINEKLFRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PU93</accession>
    <name>A0A8S1PU93_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1310067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PU93"/>
    <dbReference type="OMA" id="CQLHTIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="189" mass="22354" checksum="25E4CB0D0CE7452A" modified="2022-10-12" version="1">MYQQTSRKICVICQNTRDNTYENPECKHNYCMSCFQNKKIIKVCTEKDCNSQINQNAIIQYFEKVQKKQNQEQAAQSFNNNSSISTQSQSSIKSSMINKSKQYASPNESIQQQQYSRNNKVDLYTTLNYCAICLFYMRDDLLKQQIQVKKNYPILDCQLHTICVGCFSNYFNHQKNRQYFCEICLRLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXK3</accession>
    <name>A0A8S1PXK3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1350092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXK3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="103" mass="12116" checksum="90A5A26CC2357BBE" modified="2022-10-12" version="1">MASQAIPKDLYTYTNDESLQLMIYAIKGNHICKEQRKSFNLCRSTQLGKYVEPEFCKDNALSMIDCFLKVERYTKCKQFFQKVFEIAKIGQYAQESLEDYLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYD3</accession>
    <name>A0A8S1PYD3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1340007</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYD3"/>
    <dbReference type="OMA" id="DKNEMGQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000526">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000708">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000771">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000660">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="298"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="334"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="417"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="453"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8107483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="57735" checksum="B8304AAFDA7B4A48" modified="2022-10-12" version="1">MTDKRIDKYIFNVKDRIGQGSYAEVYRGTNEKTGEKVAIKMLSKSVINSDDYLREGLIQEIKIMQKLKSPNIVTLLDVMETNNNYYIVQEYCDGGDFDELLKKRKLLSEKDAIKFLVDVLNGFTQLIKNGIIHRDLKPANILIDKQTYKLADFGFAKCVDNFKKTMMASMVGTPLYMSPQILDHKRYNSKTDVWSIGFIFYEALFGKTPWTARSPAELLKNIRTQPLKFPTDKNQVSQETQDLIIGCLQADENKRLSWEEIYKHPAVSQYFTDFIKGNSKLEDRAQYLINDIRQMICKEKIDIHKLFADLDMSKDKALDVNELGKFLMKVDKDITREEIEYIFNKFDDDGNNQIEFEEFKKWLEDNQIITNGNAQQQQRNGGSHKKLSILPHQLKSQGSIEERANYVIEKLKLSIQKYKINLLDLFKKFDKSADQRLDKNEMGLLLKRIEPNITQEEIIACFDFFDENGDGEITFQEFQTSLSEEVGKKRQSIDNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZ75</accession>
    <name>A0A8S1PZ75_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine zipper transcription factor-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1370103</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Regulates ciliary localization of the BBSome complex. Together with the BBSome complex, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. May play a role in neurite outgrowth. May have tumor suppressor function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Self-associates. Interacts with BBS9; the interaction mediates the association of LZTL1 with the BBsome complex and regulates BBSome ciliary trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LZTFL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZ75"/>
    <dbReference type="OMA" id="DQDFHAD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903565">
        <property type="term" value="P:negative regulation of protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026157">
        <property type="entry name" value="LZTFL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21635">
        <property type="entry name" value="LEUCINE ZIPPER TRANSCRIPTION FACTOR LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21635:SF0">
        <property type="entry name" value="LEUCINE ZIPPER TRANSCRIPTION FACTOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15294">
        <property type="entry name" value="Leu_zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="147"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8108524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="235" mass="27895" checksum="813854AF64276187" modified="2022-10-12" version="1">MDGSNLTSDGQKELQSFLNFFNLKKQESMREIELEFKDFSKFQVQDTLYNKDDVLELLKKLQSSLEVLMNKEVSKIIYMCGVYVKIFLSVCSDQDFHADFNFIENVKIIEQMKILEKGGTIVEDKPLQSKIRYSRLPTIDTVLQKQMEEMSKENDFLKQYNQKLQNELVLLKKEQVLDADQVGTYQDQINQLKYENEQLKQEMEKKLNDCVQFKTLKQMIQEKNQQIKELQMKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7J5</accession>
    <name>A0A8S1Q7J5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytidyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1460088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8111084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7J5"/>
    <dbReference type="OMA" id="YLKVYEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009218">
        <property type="entry name" value="HD_phosphohydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21174">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21174:SF0">
        <property type="entry name" value="HD PHOSPHOHYDROLASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="1">
        <location>
            <begin position="132"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="475" mass="56314" checksum="EC79638227FDA3EB" modified="2022-10-12" version="1">MKHHAKLLILCSFNSIGYQLLDQKIEEYLKTNQEEIVLYLVGTPITYLKIYEQILFHLYGRIKQYPTKFIVIFDIFNKELRNKYQFDLVINLGTNLQGDFNIQLKINHEIQKIQFQQEGYTVNQFQRGANSGTFDHLHIGHKMLLSLSLLAVSQHLTIGITSEILLQKKKLRGFIQSYNTRVRCVKEFCSMFRPDIELQFSELIDSNGLKGQYEILITTQDCQNSIENINNLRKQEGLNEINRYIMGIIENQNHQGDGKISSTQFREQISQKNRLTEYEYLELRTEWLSVSNNLYWFESILVDYYSQPQRYYHTLRHIYDMLKQLKGIKNIRNVKLATFFHDIIYYPKIHDNEEKSCLLFQQFAKEGNIDTDIVSIFILQTKSHQTDVPFLPENDLSDLMIYLDADMSILESDAYPIYAQDIRKEYSHLSDQEYKTNRIAVLQKFLQSKIFNLRNEEKARKNIQEEIDILQNWVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8K7</accession>
    <name>A0A8S1Q8K7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1480108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8K7"/>
    <dbReference type="OMA" id="HMYHGLP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14726">
        <property type="entry name" value="TraB_PrgY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046345">
        <property type="entry name" value="TraB_PrgY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530">
        <property type="entry name" value="PHEROMONE SHUTDOWN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530:SF7">
        <property type="entry name" value="TRAB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="411" mass="48304" checksum="5CB697F6665B40E4" modified="2022-10-12" version="1">MFGQVVHKFQRNQNHLLSKFLRAGNRSNPFENTLTILEDPAKHRKLYLIGSTHASTMLAYRTQKLINEVKPDSVYVQTNEEWWNIVKNIEGVTSQSELNVYNDVLRGAYKWELESGVRNFRNTVFWARLYSWLGLMQFLKAFNSDFHPFIPGLEVKYAIEQARAQGANVIFGGLEITNDDLLSLKTEPRFDPLSVSLNFLKLLSQIRWRRERQDLAFQLAVLGGEQFAESIDKYRASWFVKLFEKLSPHQKKILIDRKDIEIFETLYRHTPGKTIVAVVNQWHTMGIESHWRHTTKTQINTEPINPIGDMDLEALNEDLVVNDFLRQFTSRLTKSEPASTRAYITQYYKDVFEYERFRHVEFDGHSDPHQFHGGSPFEGGYQHHHPQLPADVRKQIEENWKKQYAHHHEHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8P9</accession>
    <name>A0A8S1Q8P9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1490117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8P9"/>
    <dbReference type="OMA" id="VMLCISN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="207" mass="23760" checksum="F4BFADC1C3D01466" modified="2022-10-12" version="1">MSDSQKIVGIVFSKLESKRSHPTYIVETKAGNLIYRQAVNLPSEQIEKFNLIFSKQIADKLYPSQDKRLFYKGTELDIPDVEFDDENDQEDSKSTKQQCNCLSKQTSKSPNVQSLQQPLQLPSKSTIKLSSNTILTYDKIDEEFKQASRIDCNLIKDIKNWEQAKISKKKQVMLCISNNCFVKLADLRFKNPSLVLDFVLNNCILVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8W5</accession>
    <name>A0A8S1Q8W5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1490135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8W5"/>
    <dbReference type="OMA" id="LYFIAYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="494" mass="59261" checksum="050B769CF268C74D" modified="2022-10-12" version="1">MNKDIISDPNSNQKTSNMLHFMFNSKKKSNKFKRVLPQLSITENLNNSIPRSRIFSLNHEPTGFITSRIDLIQFTGDYCYKALNQQTLSLKNQRDRIPDTIEKSIEGCPSVHSYQTTKFKCPSMLESIRQRRNYNLQKYLDDNLTVFCDIIKKKDMEKLISFLNYPFQYSQEQNLLETFCELLYFIAYYGNTILQLNKSTFLFKDCLKLSELCRNHQLKVKILIQLFSIAKQLKQYDKAHKFILKALSYAWADNMDDYEIDCYDKLGICYFYMGDINKANHLHNKWVKCEIEPRDSYYRITSKEFILLYEKFQPFCKEFDDKISRYIHIPFINIKTGQSFDSNSTLKYNTCEAIPLINEILYGYEYTEYFLDYHYIEQAQKKHLKKSQLPRRALEIMQKYDKNKDKYIFDHKIHENPIYKLSLQEKVNYRKTKFYSLDSVQQNIQKYISEQREPFKKSEKIYVKESQRKDLSPQNGNQLYQHFRKILVSIINQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCW4</accession>
    <name>A0A8S1QCW4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1540137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1663" mass="194015" checksum="2CF4BBF6FEF46B3D" modified="2022-10-12" version="1">MLRNIQQSVYNKVRDYTNPFNLLIPWLNAIFKKYMGNFFNEEIKKEQFTSMGLKKLTLKVSSLNSQMAPNIPFVLESGSISLLKLDLHKLTCLIEEVEITLNFKTIEQYRLFVQTYTKQKMTADKLQKIKDEIIKAYKVKSETSNNGNQNAWNIIRQFLQKITFTVNKLVLHIVEPIYNDKLSLAINQISMRVRKLQEEKDQMVSTMVIGGCSLHINEQNQLFDINNFQKESCIFSLQQYYTNKYDQHVVIHLKTELKERLKFNSTLCIRLMQMNFNYKQFQIAIRMIMSILEHNKLIQSQDKTIIESIKQEIPQFQLTWCESQVYYDYCDQIGEVKIDEIDGKSLSDEATVEFNEDLEQALEKSQFNEEEGNDQFEESQIEGFNQSVLNQSNLGDLNQSTLKQSIFSMSVFKELINDEDDKQGLDELDEGQLIFKLKIQKIRVRLSKNNIIDKWNLDSLKKTNYYELEIDQLNWLMTKEITQQQITTIKQTKIEIPIIQLFHYEYCNILSEKTFDDQNTEDENQDIFQSINEGFSNNSQTIENQVFHSCYNLSQNDVLEKIQQKKNEFIKEQGNYYYKKKCILQLYDGIKESSYDGIKEEKQLITKVDNQFIYANDKIISQGNGLQIQMHHLNNSLAISIKQIDVNLNIDEILEFIEIFKIKHEKKQIKSIPNLNLITIHHIQFDIQIQEADYIEFYYLQQIIKNDPNSSSVIHQQQKISYFTFKFIDLQIQQSNSGFITFNSITCSMKDYDRIKSIFHNELIAEIGAQEHSQILIRPIIKLMETKEIRLAIPKIEIFINMSYMEYVKTLFTVIKDFQKKLQLLSKDKKENKQIIKLLFDEIIVKINLDQQTNTQQARFILHASKLAAEIIGKDEKMFTLYNCLVIDTNWNCKRPKHDIPIYGSSNDIKTDQPLQILHFRNQKYQANQVLFYKQERFNREGFKTLVQNETIKSEPDMAFQYTAIKLKNNQYLNPDCLDALYGKTNLLSIKINQNKKIEIQYQHGIIQVDEQWIETITTIQDLAKKWEQTLIPKKNTLPQNKSIPNFTFTIQNIWLNYLPTYRKSTLLKQYLMKKEPIKSIIAIQPEQLYYSWIRVLIRIKSATVSSDLQAHLSSAQIYVKSTSKDIKHANLSFLNPILLVPPQIGLFDENRKFSDGFQIDKDDQGLLRLLCFQKLFKIKGLTISGNTINIGQITGNLKKDSIISLWDITSMTVEKIKKIIPEQSNDKQIFVLQEGRQYTPNDNLNIFEDDEIELPAIENTPNKKQFEIILKSFEIKLTVGQHFPPEVFSDTNPINTECSQHNQEQYYLEHSNLLHLEEFPQINDQQCSSILFSLKELKLVCCGPTQKEINQFYLTLLQFQITDRIQNSKYSKLIGKEPNHEDINFAEVFYQIDKINNSINLEAKIVPIRVCISGAALEFLLETLNSPEQLNPKVDLLSKLNVGNDKESIIEFCVQEGYQVTFSRISEIKKSIKVNFLNIERIYCYITFDGSGVKLDGKLKGLVRLASYDSLLIFLKRAQIHNLVFEKDSDFIKFMFEQYQHLLRNDSQLIQNLITSFKAFQNITNIVLGIQTMFMTIIRQGIVTGVCSGSIPLAKALGDEIVYLAKKPIEVCSTMTEGIGLKMANVVFSPIERVLDQFSALTTSDRIPKEFLKKNNVDQTNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFS5</accession>
    <name>A0A8S1QFS5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1580018</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000163">
        <property type="protein sequence ID" value="CAD8114084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFS5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="298"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="241"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8114084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="42424" checksum="33225D410C7000CF" modified="2022-10-12" version="1">MSDLQDINPYPQIDEQHDSNHQIEEPTASQAIPVNQQNIVLDNCTTTRTISEASSEERKQFLKQSLRALAIQQIFQFSLALGFAFVDDWGFCSNFFLFLFITLSLHLFIATYKARGMKKEERVMKFQWLGWMICYSFAITLFSTLLNVLFFGWFFYLPFLTTSFLLPIITLGMIVHISSGSVCINTKQYTIIFFVLIFFLDLSISVFDEGYIHFGYLIGEIVAYLYGLYLTLINVQIQYPVNDLSLNIEQEETKLDQVMNLLDRGTQKFEVGSIPIDAQEITMEEIKKQSNTQRLLKLAILQAFCIFFYMVPILYLVLMIWLMVVAHKANTKFVQIGKKYEVKLNEQYVAPILINLDILGGLKHAIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFV3</accession>
    <name>A0A8S1QFV3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1610093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFV3"/>
    <dbReference type="OMA" id="HRNNMIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="109" mass="12792" checksum="9C4BDB4480B06703" modified="2022-10-12" version="1">MKQGTKQLKISYHKMSSEVKQTLVYLICLKGFKIKHAAQKLNIKYAAAKSIFLYHRNNMIKNEQINDSTKRCMYRTITKEIIRFRIITKIAGEFVNSKTEHQISTQIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFY1</accession>
    <name>A0A8S1QFY1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1580079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000163">
        <property type="protein sequence ID" value="CAD8114196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFY1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="119" mass="14008" checksum="134F58E39D5D5E9F" modified="2022-10-12" version="1">MAITMFQQNENIFKIKIDEFNDLCQQNALTQSKHLVFEKMKLVFLQAEQKFKFMKLNLDHLQIFIKKTLHKFRKKLRDLQSNLDKIPLSNISSNSKKQPPSQIKGIQIVRNSGFSVIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QK31</accession>
    <name>A0A8S1QK31_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1670071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000176">
        <property type="protein sequence ID" value="CAD8116019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QK31"/>
    <dbReference type="OMA" id="PIMNYRY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="211" mass="25439" checksum="F21B6945019D41C5" modified="2022-10-12" version="1">MQRIKQQNNAQELMIHKRRLSDIQQRKSSIPKLDKTIDIYKQNRYITARKQEINRWEGIMKQNQKLLKKLNNVESSFQKSVNNKQNILVFKIINSQCQKSIEFKENLQKENVRMYQKLQKITSTFEKPNKQYSRITKSNQKLDKWKQPNSQEKSFNVQPIMNYRYQGKSFTKQKSIHHSGDEKQQIMSIIQKYHQVLDQLLPIIKPNKFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QM04</accession>
    <name>A0A8S1QM04_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleosome assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1690020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000178">
        <property type="protein sequence ID" value="CAD8116081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QM04"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="282"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8116081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="36971" checksum="4107414A0D759C3B" modified="2022-10-12" version="1">MENKELEQEIKKKISNIPLKDRAKVTALRYYLNKCKQADTDMETTCDNIQSKYDKLSMPIYQRADEIILGTAQLNEQNLGLAAKLLSEEELQQFNQIPKESKAIPDYWLKVFKNADLQELPLTERDCEAFKFLQKIEFTLAENDSDFFLKFHFAENPFFKQTILEKKFYYEDDELTKVEGTKIEWHDGKNLTKKLIKKKQRNKKTGQFRVISKEVDDESFFLFFRSIDISNKEKFDKLPEDEQISLQGQLDVDQDVGREIVDELLPYSLEYFLGIKEFKTEDDAGAEGEEDDSEDDEEVEVQTKGQNKGKDKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QM27</accession>
    <name>A0A8S1QM27_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1870011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000196">
        <property type="protein sequence ID" value="CAD8117058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QM27"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="15948" checksum="7BA78E6E85118BEE" modified="2022-10-12" version="1">MSNQCKGQRKLKLLMKPIIRLDYYNHYKEIHNHSNKLYYITRKLIINNLNKSQNYNTYQMREKNQILNRNIQLIINQQNHNKQIRYSQNNFYNWNKNIYQNILNQIENKLLKKDGQIKLIFYKMRIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNF9</accession>
    <name>A0A8S1QNF9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1950008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000204">
        <property type="protein sequence ID" value="CAD8117313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNF9"/>
    <dbReference type="OMA" id="VFHYFII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36851:SF1">
        <property type="entry name" value="GLYCO_TRANS_2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36851">
        <property type="entry name" value="UNNAMED PRODUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="61658" checksum="61A08429E91E4653" modified="2022-10-12" version="1">MDQQNQSQRVVICSELPENPIATKMTQKEDKTCGGRFRWNLIINGIIIIMAAFPFYLPLEATLGINCFYASLWMIFTILAALTQSKIHQTMKKIQNNMEQPSLPNSSQIKFILITFIYKEPLELLLKTLQNVSQQRIAKEIIMLVCMEEKTPDKETKIKSVYEQFKDCFGQLIITVHPYGTPGEIPGKCSNNNYGIRSVLAHLKKSDPNLDPNKYFVTNFDVDTIFHKNFLDIQMMNILQEKDRNNFVWQPILFYNWGLDKLSVFTRITGLARNMLMMGALIPFNINIMSVYTASLQLYIEGDFCHPTYQMEDIICYIRWLTLSKRSLKIKPIYCPTISGPTSGSTIWQEFIEWVRQNKRWSVGSAEVFHYFIIKASRINFCSAFLWACNYLNYYASFICVQSLLLITTTIRLFAMETDPILQQYFIIPLIAVYICLFFMIFMNKIAVKYLLKDIVIEKIPIWKDFFHWILSLIVMVGYGLTVFYGFWEIFFCGKGVCTHEPSKKKVLDNIVQRKDETTVPIQELQTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QR79</accession>
    <name>A0A8S1QR79_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2040018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000213">
        <property type="protein sequence ID" value="CAD8117612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QR79"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="161" mass="18711" checksum="6DD93D340DB1A982" modified="2022-10-12" version="1">MNPSKRDGSIMNCFIDVESLMSCNKQILQDYLQQLAVFQFIQEQSSLQLPQLNPISPKEFQFMKQCISQNKGLKFDLISDKWIKKNGTSEMLSNLWSQQNADLVLNFGENRVIPFNKAYSNIQILIKFQGSILKTKHTYLDSICFSRNIKLIIDQFIVIQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JR69</accession>
    <name>A0A8S1JR69_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JR69"/>
    <dbReference type="OMA" id="KEELCYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="104" mass="12700" checksum="EEBE2A677D8D0174" modified="2022-10-12" version="1">MFHQQPIIGELINNHVDIVNQLIKQKEELCYQNSKNMDAFADCMLIKEKRFKKLQMKSEIFIRFCELKYENCLQEKDEQSCIKRINNLFDDFNYHMKYENVQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JR95</accession>
    <name>A0A8S1JR95_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JR95"/>
    <dbReference type="OMA" id="TISCIFY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="265" mass="30450" checksum="DCF2733D24988872" modified="2022-10-12" version="1">MLNIDYLDSLIGQNNSSKLLMAINAIGCLGIMVILKELEPNYVTLSIQYGFILLLDSQRSQYVFESQYDALMMALIGGLNAIGYFTLFAALRKIDLLVIMLVFNFKSLFVHSIKLQNNLSSILFLFGFTLNLMSNDRILAVWALVFGQVCQTGSGMIQERYKFHIQNLQLYKGVFMIMMSLFNFGFYSDKIENSFFVYLASFPVLIIFTFFQWLKYEVKIPNEMAESAIIGFQLLVLLLHHSFKILPTISCIFYFLGIFSMCIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSE5</accession>
    <name>A0A8S1JSE5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="29677" checksum="C3544760A1EBBFDE" modified="2022-10-12" version="1">MNVIAEDGCFNNQYECSKDYIHCYNSLCIISYLETNLLHFLNNQCISLCGDDYXKSEFDVIQSHYSNNAIKNKETVLIVNMNAILSFYIVILEDVLNVMKIKVGTFKLMENVIPFVEMELLPIQVKCVMMEMVTHKMDAINVNFLVINFIILLQILSVQIVKKAIDQYQTNVFHYVMMTYQCILNNVKIIINQLLMVVLIANFNVEFIAKYCICQQCNEESDWQIQKVRTYQSVFQLSLQIMLNQMRYQLFIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVU7</accession>
    <name>A0A8S1JVU7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVU7"/>
    <dbReference type="OMA" id="YHELCED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019351">
        <property type="entry name" value="DUF2039"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22876:SF5">
        <property type="entry name" value="CHROMOSOME 9 OPEN READING FRAME 85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22876">
        <property type="entry name" value="ZGC:101016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10217">
        <property type="entry name" value="DUF2039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="291" mass="34737" checksum="D060007102910055" modified="2022-10-12" version="1">MKKGAPKYQNKIAFKMRFHDHYTEKVQNASLDRLCELCLAQIEWKIKYGKYKTPHDLSRCYKCEKKNIFKAYRRLCDACSDELKQCSKCTEPIEINDDSSSGEEMDETLKCLRERSKRTVLRKIEKGEITWDKHNKKFIDCETGEQMIVQYKKDYIDEEDEQLWEDNDNDDDDDDDDDVDSDDDDENSDESDEDNNTKQNKKKVQFQKQQKGQKQKTEQTKKSKAKKVESSDDESFSDESDEENQFNQIQKINKKDPSIYSDGSNEEERINRREKNFRFYFDQKNQLNQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWM1</accession>
    <name>A0A8S1JWM1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0080549</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="EATHYIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="396"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="444"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="492"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="534"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="576"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="5">
        <location>
            <begin position="586"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="618"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="660"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="702"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="744"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="786"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="828"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="870"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="912"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="954"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="996"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1038"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1080"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1122"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1164"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8044980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1283" mass="141671" checksum="3F3A2C69316E4E6C" modified="2022-10-12" version="1">MINCTHHFQNPITLICKAPHICKFQRKLCCECFDEHGVDVKHILSLKKFQEIALKKLNDSKLNESSELTKQRMALKSAISQTDQTLKKIWKELSQLIKQVYDWIEKENQSYLNLINENTNLAESSYSDIEKLVNIVEGSTLNDWNAEKISYMKELDKTKSWWDQHIKVFIEKSNKGIQQIQSLIKEHEEEIYLMKEDFYEMLTYIQNIDETLYQKILDTLRKEKASDIIGFLSNTNNHHFYESLNCKQVNIKDVKKQVKKIANVLRNIQDHQFSKDNYSSETYEKARQDLIKKLKDNKGIIDFIKFIVLLTSIDVKFIQCGSNGLSLLVAMKVDIRNQSFENIRIKNTSLIGGNFVRCNLNQSEFDNVDISGLNLNGAQLLNCKWKNLKIHELNKLNGYYGPVSSVCFSPDGKTLASGGGCIIGGGDNSIRFWDVITGQQKAKLDGHEHDVMSVCFSPDGNMLASGGGSIFGNGDNSIRLWDVKSGQQKAKLDSHSNVVMSVCFSPDGNTLASGSWDISICIWEVKKGQLTAKLDGHSNLVMSVCFSPDGNTLASSSWDNSIRIWDIKTGQQIAKLDGHQYQVNSVCFSPDGNTLASGSNDKSIRLWDIKTRQQKAKLDGHTSTVYSVCFSPGENTLASGSYDNSIRLWDVKTGQQKAKLDGHSNYVMAVCFSPDGNTLASGSYDNSIRIFDVKTGQQIARFDGHSNCVRSVCFSPDGNILASGSDDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHEYGVVSVCFSPDGNILASGSSDNSIRLWDVKTGQEIQSSDKSYKDILAQFKVPLQSSCPSQEATHYINTLLISQKAIFQAKGALILKGEFINQSGIDLKTLFKQKGSCFLEDLKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JY98</accession>
    <name>A0A8S1JY98_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0120036</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JY98"/>
    <dbReference type="OMA" id="YAKSICG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000537">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000048">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="50"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="307"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8047722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="379" mass="44305" checksum="0B7A038711307C9E" modified="2022-10-12" version="1">MGLVCFKEGPQVVLGADPIEQRDEEEENEEVISEYPSQDENGKKISLNQFIMLRNLGKGSYGKVMLVQHTVSLKLYAMKVLIKKAVKTQTQKKHVQTERKVLEITNHTFITKLYYAFQTQTKLYLIMEFVSGGELFYHLKLKGRMTEKKMKFYAAEILIGLDYLHKQNIIYRDLKPENILLDSEGHIKLCDFGLSKICSWGDMYAKSICGTFEYIAPEIILEEIYTKSCDWWTYGALLYDMLTGKPPFYQLTKNQIKEYAIGKDIEIPNYLSEEAIDLLKKLLNRNPDKRLGGQRGAQEIYEHPFFKDVDFQKIERKEIEPPFEMQNERPFKFFDQNLIRKSSVNDTPVNELGRFQEFSNFTYNGDQLSSEYNQLLITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K045</accession>
    <name>A0A8S1K045_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0120470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K045"/>
    <dbReference type="OMA" id="KYLWYSE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="261"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8048448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="391" mass="46101" checksum="52D147B643BEECFB" modified="2022-10-12" version="1">MDQSDETEITLQVCDPKVTPGINKYIVYTVKGMDKNGQFDVQRRFSDFKTIRSFLLTKWPGCYIPPVPPRKAFGNMEQQFIDERRLMLEDFLKKIATLKYLWYSEEFQIFLKTPGEIEKALLSIPKITNDEIINKYQESFSELSGKEINREIIQKINDFKAYIKKIQPMVQNFKQIAKNIAEAKSAHQTSLIHFITIFVPEYENNVLLEYAEQKEKLIFANLIQNEEVKGLINQYCDLLKDPKFQQLYQEVRKEGKQLKAFLETFEEREKYEQQKANAEQRQKELQLQLQEVLAGKTTIRTLFSKGKKEDNVAKLQQQIAEVGKEIESYQFICDILTVLVGYVEIDKFKGEKQYQYYCMIKNIISYEISLSQIAEQFWSKVSESENMLQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K186</accession>
    <name>A0A8S1K186_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0130178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K186"/>
    <dbReference type="OMA" id="NRIMQAP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="476" mass="55845" checksum="C623C59E67D81985" modified="2022-10-12" version="1">MFKPKKGQQQQQQQPFYLSWFTNQGENLKRGGTAPQQTRKERLQLKSPYQQPLTTVIHMAPNPYNRIMQAPKTNSNIQQISKIYAQKSQLSKRKSQEYERQRYDFKPQSQQSRAFTQQGIRPLRLNDGRPQTQQNLIENKLCCDNLDSDEEQQEQLTPRDDKEDIDIDNNGANQYINDCFSNESNEAADSEDHQNFQLHIQNDEQIQNKSLIKEEPITSLRKTNIQINRPQTSEGGKRKRFMNSLNQTDQNYEKKKETECELLEYEANIILEEKDNQEIIDINFKSSPKELISIRSGYRRKQNDLNELLETNNELRPPSRHKTPPKATGLELPLENVVNNIQRESVDIPIKNMYAQINDEKIQEVNDVDFDLDFFKMNNKMNFKKFQQNDIQGYHTDDGNKYNQQRHPKSANLKQNQLRSPLNSQITSAHLRNEAQIVIKYNNSSAIQRNDEPIKVPFQTSLGQDFLRLFANNHME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K265</accession>
    <name>A0A8S1K265_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0130073</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K265"/>
    <dbReference type="OMA" id="ENMICEG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000221">
        <property type="entry name" value="G1/S-specific cyclin e2-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="147"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8048773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="426" mass="51545" checksum="A505AD77CB138543" modified="2022-10-12" version="1">MQNNGKKSISRPKKCQKQRNLRDKNNCNSVNLKSNQAACQQEQATTKKVKNEKTISLIMIEEIPFTQNRIQQLNSQKQENLICEEFSKNLYSFTFNNLLEENQDNESDTLIEFMRQQVKFEEIYSYQKNYLKNQNEIQSHMVAILFDWLIEVAHSFHFKRETFYLSINYIERFLHRQPNISITKFQFLGVVAIFTAHKCEEIYPKALKDFHRLIQDQYTIKDMEEMEVSILKCLDFRMNPNTPIFWLNYYTKLWDEFIITKELNVLFKDKTNQSYYRYRELVQLFDVCLIDYKYKKNEKLTALSLIYLVIAKSLQIFQDYRTIAISQLEILNFFDSNHEYNLLFRQFIKQSDLYLHFDKKIEYEDLKDVIFETTKYFILRFDNTLPRVLLNNQELIDELQHEELLSFQTYNRNTIKTINLLLQSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2N8</accession>
    <name>A0A8S1K2N8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0130250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2N8"/>
    <dbReference type="OMA" id="IRDWCKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8049137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="572" mass="68506" checksum="21BA365D55E7984A" modified="2022-10-12" version="1">MYFRERRALSNQKQYQGYEDTKIQLDLIDKFINPSYEIPISQQPSQKSGIRQLNQMDKSYQIPKVVVYPEEKLNEEVAQMQQALDKQSQTLKLLLKEQKEINRTQVLKSKLDYLKTQVQTLHLKLPQLPQYDQFYEIKHEINSLRYSFFNQVHQPQQPFIQPPQIIYQQLPQPFSQQYFQQAYLPFNQQQSHFGSQRDYHPFQHLINPYYNPSNPKSQRFNTKDKYPNSKGKVQKKTQTKTQNSVSKPFHSERNSRQSIPLISKGLNQKTKQSFFMDNAVFQVQQKIKKFKLKNIFNFVRYAMRWKIYCRPNKILWRKLYRHSVECKAIILKTSYPVALKRIRDWCKMVLAKVENYLSKIKEIDFINPEKILTDKEIDQSFMQLTISTKYLITSLLTYCTSDFIIPELKFLSYLQFFDEPNIDRGLFISKRVLFWKENQLDMTKTQQQMIIGDLVIIVHILPALMDISGSYFLIKCMVSLIQIHFMKQFDLQIINPNPSYRLIQLNVKEYVNGKFSLKLQKVENFDDDRYIIGVYEEQQFQQFFQKRPYFQDDMQKTLSEIHTNLLQALISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3T1</accession>
    <name>A0A8S1K3T1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3T1"/>
    <dbReference type="OMA" id="CRNCQFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="197"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="318" mass="36521" checksum="EFD0B127F0E121BB" modified="2022-10-12" version="1">MQSVCNKFVMDMTDTRMGPEQKCRNCQFMKKEHTQSNSQQLPSNQNKVSDLMKKFSGPNNQVQEKFQGAVKSSVQQKKLEEKIPPQIQVSQTTNQSDQSNVSIRKIESQTISNESSSQTEQNKNQIQLEKQQSNTSKLDDINQIQQPPKQQVQIETAPFQTKLANNPFLKNDKQSQNQVAFSKPSVQNKIPGDEQKQQQTIQNKFQQQNEQKKNVVSEDQNQRSNFQEIKNAFAKQSPLVQEDSTTGYKQSSNKQQQIGNNFQGRPMNMPIIGFGPPPVKKISNQQEPESSLEQQMIERPFIQQKKNKRTIEEFSDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6J0</accession>
    <name>A0A8S1K6J0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0170004</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6J0"/>
    <dbReference type="OMA" id="MFISTTY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000020">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032451">
        <property type="entry name" value="SMARCC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16495">
        <property type="entry name" value="SWIRM-assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SWIRM" evidence="6">
        <location>
            <begin position="165"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="532"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8050371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="623" mass="72909" checksum="2A927CC257D667C3" modified="2022-10-12" version="1">MNQQDQLVKITLIKVPDGYQVEEKIYTKIKIPKTFLDQQKITQPKPAQKQVVTKPPPQIKQTLPPPIQPVNIHPPMPPQNNLVQSQMDNIPSKLIWNSSPTPQNPHQVKQMPPQTQWFAQPPLPQIHQPPPQIQQPPQPQIPIPIQQPQESQKQEIRRQLSKHSLIIPSCANWFQMESINEIEKEHFPEFFSNQHVSKTPELYKKQRNFIVNLYRSQPSTYLTTIACRKVLAGDACTISRIHGFLQYWGLINYSVDPDTCPNKVLPQQALTKGLYRTLQLNAKDELDEKSDLNQYEITLINAIKIFSKRYRPGCSFCGILCGLQWYSEKEQTQKEKQDQDKVENVKEVENKQKNSIKLDLCMKCFSNNNFPNSLTSEDFQLTNLEQKFQQLHILSDQPRSILNDQEVSYLISIIQETSDSNWEKLAQQLNQTFSTSHTEEELILHFLQYPIDHLIEIDKVIDSKECLEKLSINDISSRIAFEEPNVFSDQKNLVSFHLSIFKKLLNNLNKKDSEQFLKLDEVKSNSNNEDIIEEEKRVIQMNQETLERAKSLQKREEEKLNNHINLLISLQMEKLEHKLTFLEEYEKLILYEKQQLEICQKQTLAERLVIVQQKLQYYSDNNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6W7</accession>
    <name>A0A8S1K6W7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serine incorporator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0170102</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6W7"/>
    <dbReference type="OMA" id="KSPQWWD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005016">
        <property type="entry name" value="TDE1/TMS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383">
        <property type="entry name" value="SERINE INCORPORATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383:SF9">
        <property type="entry name" value="SERINE INCORPORATOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03348">
        <property type="entry name" value="Serinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035829997" description="Serine incorporator" evidence="8">
        <location>
            <begin position="19"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8050527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="50361" checksum="773E1B0939504C91" modified="2022-10-12" version="1">MCLTATMDALCCTGMVCCKTICCCCKSCCGSTFKEQIKLAYILLNYTTIFVTMIFCYFLQGLFDNYLKFIQCPTDDNSQCLGVSSIYRICFVLMWMYIFLMIFMLIRGEIAKIANESLWPLKFLFIAIFFFGTLFIHNSFFKGYVYFAIVISGLFMVFQIIMLIDVFYLWGQSWISIYDNGGEYMKYILIITTAILYGGALTLNIFNFIWFSGCGTNIFMNIFTIILIVGATGVQLLGWNPQGSLLTSGAMAIYIVFQAYQAQSSWPESQCNSLSKSEGTRIIEIVVGILLTIVSLLYLTFGTSNSSSINLVQLESKDEKLENQQREANQDEEQQLLQQQQQQQKLEEAKELVKQAEMLPYTTNQYLMFHFIMFITIIYMMILLTNWSEPNFNENSFEAYQPNKLAYWVKMVTSWLAALLYIWTLIAPHIFPDRDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7F9</accession>
    <name>A0A8S1K7F9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0170189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7F9"/>
    <dbReference type="OMA" id="QRMISLV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="174" mass="20681" checksum="569D2F553DC904FF" modified="2022-10-12" version="1">MLKKNTKLNRIGMAYNLNSIEERKQIMELKRQGKHSKEIAKIMNKKIKSIDSVKSDERKSEYKEFIQKMISYVLKYFQDKIEYDMIDKKSLSKKIRKFINQTLSSNQNDQFRLLIPQSRKESNQRRIKEKIVDSVVTCLLQVPNSHDNVAKTLQNQNQKDTQIIEENKETLDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7Q5</accession>
    <name>A0A8S1K7Q5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">JAB1/MPN/MOV34 metalloenzyme domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7Q5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JAB1/MPN/MOV34 metalloenzyme" evidence="1">
        <location>
            <begin position="12"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="259" mass="30612" checksum="A497997F2A154433" modified="2022-10-12" version="1">MLGEQSILKAFNVKLDTIAAMKIIKLANDNPIIHQYGQLLGIFEGETIEVTNCFPIINMDEEQDQQYEYLDYAKEHHLDFHKLGLYIITDKNKYFTLGQFDDINRLQFYVKYTFILVYSLQLAKSGHSYPLEAFLKYSQIYSKDEFSMLDIKNDKLYRQLPIELLKTPLTEAILHQYRTSIEEKFEYEETKFTSNISNYGRQLIETIEEQVNLSDKIISSTLNKKDVDNPELLEYLISMNKLYYLAEAIKKRNIIYNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8Z6</accession>
    <name>A0A8S1K8Z6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8051023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8Z6"/>
    <dbReference type="OMA" id="SHITRMW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="691" mass="82004" checksum="A8B436D21D3C24F0" modified="2022-10-12" version="1">MYIKIKKGVKQFDLFGVELNLNMKQKDKYTTACGGLSSIIMIFLLSFIFINRLISIITKSEYTIKVQTIQSANPNFTTMNVQNFMLAIKLEDPLKNYYNNSQKNLFSIQLHQYIQKTSGDGSKSREQLKEFKLEQCTVKHFQNIDFQQNAYLEKQLQYYVCLPLSFELQLQGGYNTETLLYPKLLIQICKDDVNCYTEEEIKKAQLDENTTITLSTLIKSALFVSNYTDDVLHQYINSDFYLQSNLQQSIQTDLFFQHNKVQIDDNLLSLMATTQEQDYWSFILNDNRQFNKKDVNLTTLFEINLRINQQNQIITKTAYRLDQFLSYLGGILKFFTAIFGLFAIKYNLLSMRISLANILYDFNIPQQSQGRLQFSYDRLLNFIQYKINRVDELLDKLKTYTFQVIKLSHITRMWSNFSSSQKIQRQQQSQEQAEVTEQKITQHDIYQFTKKLMEYKENFMENLIQKILDTRNQLKLDLNFYVYILLCCSCFKKIWMTRQLLIQCDQMIKRDLDIITILSKIQQIDKLKKTLLDQNQISVFNYTPKPVVFIDKQYQIITEDQSRPNNLIIQANKQKRARVINKRIRFNTTKKFLKIYDSYLKLKENKNEINYRLTQMLGPTLELMFQKYHEIQLAAQIKEDLNKTQLNIQQQSKISEQSIADNNQKVDQKIQIYQYIKPKMLEDDGEEIEYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KA56</accession>
    <name>A0A8S1KA56_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0110439</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KA56"/>
    <dbReference type="OMA" id="YFNREAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000200">
        <property type="entry name" value="Kinesin-like protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="5"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="477"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="92"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8047458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="756" mass="88463" checksum="D3D9D64CAA35A0FD" modified="2022-10-12" version="1">MLLDNIKVAIRIRPFNKRELETSDNSTLKVIDQETLIAIPPVEAEAKQGKKTYKFNWIFDKIASQKDIFEKTLEYQVLSVLDGINVTLFSYGASGTGKTHTLMKLNEDHGLLIRALNQIFQTIHNKKRKIQMKFSYLEFQQEQLYDLLNNMNSNLDIRDDVEKGVIINGMREIDIASTQEVINLIQYGKRQKSTKQHEILIFTNYIQDIIGQNNEIQVSKFIVADLAWFDKSGQKSASLQVLNDCISLLSEAQNKKIQPFIPYRNSKLTKILKDSFGGNSKTLIIGCVSPSIMNYEETIQTLEYCSLATGIYNQGSIKNLQQSTHQDNFKQVYDQLINENVELKKLLNNNNKSSQKPINNDKEQTKLYEENILEHFNNEQDINQSIFKYQWEIEQIKFTINENQENLQRIDGIDRKAADSLRYQIEQDQKQLHNYQEQLIKFQKQTSNFIIQRRALEENVNQSNLQNSYKLYLINLIEKHILRLEVYEIKLKEQINELHRQQHERIMAIQRSQINLRDRIIADQRKHIIQHGKNQSVQHYSQIDTTTEVIDGTKAKLKHYPLPKVKDHYFNREAYLQRKESPKSIFSPRLQTPLQLKDIKPYVPKTPHTNILENLSTPILPKSNPFQKAPPQKIIDSQSMVALPLITTNRNLSKFVYDWRGGGYKFIEDQKKIPSQRELKHIIQSKQHTQQSLDKSFFTTSSRSTSAKRVSFKQNLLLPGKVHESPYVKEFINKEIQRKIKLKQLNQKMMKVTQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KA75</accession>
    <name>A0A8S1KA75_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0180099</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KA75"/>
    <dbReference type="OMA" id="NDSSFHF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="399"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="796"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8051443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="957" mass="110867" checksum="6136D859E6AD6EE6" modified="2022-10-12" version="1">MYIKKLKDLISTTFDDYRNERYSVQHNQLILQKIWPTFIILVKFYAIIGLIYIIIYNSLYFQSFNKSYTTFYLYIQLVAIVLYVAIVERITNCLRESYKLTLIIFTFILIETSFQIFLIEIYLSNDTENKILTICQLFINLLLVLGIVRTIKLRIMLLLKFYLYFLARGIIFHFKTLQSINIIFFIILILYFWDQQLINLSKVSQFTDQTLKSIPSAVCVLDQNCQDVLFTNNFTRKLINQLNYGGLSGTKSINLLQNQGFPSQESILSSSSNEIISFFDNLTLPQSEVKEQLNCDILHRLDSKFECTDHNQINLNQALNLIKNQENETQGCIYTLRCSYNNQTSEDEYPIIVETRIKTKLQYGLNKNAILLNLYDVSPNFKSSYYKKLNQFKSQVIRSISHELRTRLNVIQGFLQVIQCNSINYDKGTNKLLRAAFNNCRIQNLIISSIINYNLIREKKLVTKTEKCKLISIIQEAIDLFQEEAEMKNIKITFQKLVNQAQNEMLDYEKFQSILIHIISNSIKFNNKGGQIIITITKDNQDKDKIIQNKENDKKILQPSQGSQLYKSSQDLNDSSFHFPQRKNTIFKQQLINPSSSFSNAPPQHTNTHLYEYYTIEIRDSGMGINQQKLEAIKNLLKNEEKAFQDLDRDSASGMMLGLRASNALIKYLSGKEEDNYITIDSILDQGTTVSIHVKCRITQLTEGFMPSDLYNKESSQIELEGKIPDIESQTFKFNIWTDPEISSRTNMINKTKRSSQINVNKKGSLQMFSNYNNSSFYKNLEGSLYKSQGCGCEQKIVIVDDEPYNLLVLESLLKQLGYQSIKADNGQQCVHLIENSISLFDEHKCRGFKAIIMDYQMPIMNGEEATKQLQFLFKDQQRIHIPVFGLTGFSGEDDLINLSQAGMKGVYIKPITLKTLEEMINNLSLIECRDTPCMSSRQFQCFELDYADDLEQLAHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAC6</accession>
    <name>A0A8S1KAC6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CSC1/OSCA1-like cytosolic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0180119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAC6"/>
    <dbReference type="OMA" id="SIGNCIW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF83">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="399"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="481"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="551"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="605"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="630"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="2">
        <location>
            <begin position="177"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8051483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="792" mass="91753" checksum="71DAEAB9E4E291CC" modified="2022-10-12" version="1">MNLQEGQNDEYEPFRRPPNFELAKNHAKANKFSYSKEIENIDNVEYCPCCGFPIIESQLSYCCRNEELAFNGAGVPLFFEFIIFVAILLFLNFLISGLYNALNNYYGAQCEINSSVCKLNYYNTYSLTNSSRQPDVILLGLDICNILAMIFFSIYYRRHLNIIATKIDEKNISISDYSIQISNLPISSTKQEIQNYFSEVKLNGSSISKLNIIQIVLGFDIQEYYQLTKLKIENELRITSILSEQQNLSMGSQTQNLEQQLDEINAKIIQFQQNPQLFKFSGVAFITYNTSTQAKQIRKYHTQTKFQYILQKLLFFYPLESTKTFQGKTPHVIRAPEPGDVIWENLGINPLIQFKYQILTNLGSLFILAICFGSILGVSVYQDKLNQSDKSSRSTSIELYITVFGLLASLIISILNNLMGLLIKKFVALELHSTQTEFNISFANKLGIAQFINTAIIPLLVNIALNDQDILTQTWKDGGLISDVCLVLIMNAIFPWLFNLLDPYFFYRQYLMRKSQNQGQNCALTQREANLLFEGQTGDLSKKYATITKTLMLSFFYAQLIPLGVLISLGFIIINYWVEKYLLVYRYSKGPTIGSQLADEQVDTYIELAILLFSIGNCIWQILLQNEIHYLVWFQLSLGIAQYLFPVDQIFDNFIKMEEPETESTFEDNQIFIWDDYGKRNPVTMQNEIEQWILQISKRKTNNLQNKKMLKYFNRVKKQKQNKQIELQESIANSLITSKFLKFLINKKQIYPLHQEFIHKLKTKTKTKNQTENQEQANYCQTINQSIQELQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KC83</accession>
    <name>A0A8S1KC83_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KC83"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="137" mass="16306" checksum="53C26BB467770AF4" modified="2022-10-12" version="1">MIISLISQLDQALIIWKTKKKREQYFLLCQGKVEIEKTFISEDDLIEINEIVNRIKIIIEINFNLHYNNLMLYKFFEYLQAYAVKKLIEIILLFTINVVILRNQKSSNYILVSIKKAQICRIKSQKIGTIQTRCYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KED6</accession>
    <name>A0A8S1KED6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KED6"/>
    <dbReference type="OMA" id="FANYPTT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="76" mass="9309" checksum="22298C4002B44069" modified="2022-10-12" version="1">MRPIPWYVRLGIDVTIFTVPILMLASYPRLGTHFLNNRRRKYRRRNFDEIQYRNDSTLSSEEVINFRKRFGFQDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KED9</accession>
    <name>A0A8S1KED9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0200360</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KED9"/>
    <dbReference type="OMA" id="HIQYRES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001190">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="8"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8053498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="294" mass="34562" checksum="08C02EF0A6753570" modified="2022-10-12" version="1">MQKIGQNFELKKKLGSGAFGDIYLGVNKKNNIEVAIKLEPVTSQCPQLRYEHEIYKRLLADNQSIDRGIPHVYYTSEEGGYTFMVMDLLGASLEEIFVSKKKKFSLKTVVMIGLQFLERIEFVHSKGIIHRDIKPDNFLIGKLNKQNKIYILDFGLAKRFIKENGHIQYRESKTLTGTARYCSLNTHLGVEQSRRDDIECMAYVLFYFLKSTLPWQNMRAKNKREKYERIMEKKMTTTYDELCKGVPKEFGQILKHARNLEFEEEPNYKYLRGLLESICQQEKITVDYQFDWCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KF39</accession>
    <name>A0A8S1KF39_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0210357</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KF39"/>
    <dbReference type="OMA" id="SNVDQKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="298"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="176"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8054220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="434" mass="50690" checksum="10F086BBB48950B2" modified="2022-10-12" version="1">MIEISISQISLKQALGNCQINIGFKCGERKEILYQQFTSTQKIIKYSQPHQYVFDTQNTKFLQFYIETPDEQGYIKLLGSSKLLINDVQGREVSHKIELEHGNIMNIVLRCTTEVRNSAKQIPIINNRIDNYHMMSVNKSEPIINKKRYGSPQPNKNQPSRSPQHKKLPPQPQPQPVVMQQQQQFLQQQPQQQLLQPTSSNLSKSSASSIKQQKILEDYKQVMQNSILDESEEFQTSPDHRIDPPQPIMEFQNKNSVTPRQSSRQIDEIQLAQYKQMVKELSLLLDVQQDCDSIVYAVQQLLQKYRAQNEQIMKIQCDNQILYSQYSSLQQQKSLIEQKSDEFRNQVKTKLKTYKSLFDENVQLKNQLKEDLQKIVDLNIQIRELKGDQQEKQLIDSEIQTLKQQIDKVASNVDQKQYDYAILTEEINLIQFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLI0</accession>
    <name>A0A8S1KLI0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLI0"/>
    <dbReference type="OMA" id="REMMEWN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="306" mass="36652" checksum="1077DE56BFD8306C" modified="2022-10-12" version="1">MQSNQNIVDTQIIGLTKSSKIAKQKQKEQRKYCTIKKEFKIIINIPKTLEIREYLRTEIDIIFSTDYQINSNLGELCTNIFTVGYKILQTDYFSDKQAFSLFPRNLQCVSLDVIFNLLEKMNLQKSKEDIKYFMLEHKKGNIQISQCTDVYYLDMYASQFPYESYLIRYDSTGSKVQKYINNMKYLYLMGITREMMEWNLNQTHMLPCAIRVESYMDIWSKMFEAVSRKSNFFTVELQNYNGQRIFVKMEQRLIYIVQENDNSMYCYLYWIYHTNPNPIIAENNYQKELDSQNPKPKQCTYKQICN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KMD0</accession>
    <name>A0A8S1KMD0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KMD0"/>
    <dbReference type="OMA" id="QIHYVES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="122" mass="14340" checksum="FE030BFD6C7D67D8" modified="2022-10-12" version="1">MQIYPQPQDETDRKLIEIPFNIKEQTQQNKHQSESIETTQKDQLSSQFKSKGLLSSDQKSCSQKKVRLDSTGQPILKKSKAHQITFSNQLQQIYYVESWKEYNSNDNQEEEKETCCPFCKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQA9</accession>
    <name>A0A8S1KQA9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0220327</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQA9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="13"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8054986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="544" mass="63248" checksum="9A2216AA6CAF93A3" modified="2022-10-12" version="1">MITQNQCIEDYRIQLNSSIGRGSYGEVFDCKSIKYPQLSLCAKIINAQVLEKYKNIEKMISQLVSQNKQNQNLVTIYDVKHMNEDKKLIIIMEKCESNLQKIINFQKKFNDLEVSNFIDQFLNGYQVLYDNNIIHRDIKPENILIKQKTYKLSDFGTGKIYKANDFKITKIGTPVYTAPEINSLVQDTELDDRFQKIKFNQHSKSQVDIFSLGIILYQMVYGELPFEQKQTSIIEFAKRIKQNPLQLTGQSQHKKIIEQMLIYYPDERMTFQCLYNIIAEKKNTKISYPISFKQKDNQIKQPQVSATMSSLNQNGGNQNGLNQIIINQNGGIQNISKKNEINQTGGNQNRVNQNIINLNGLNQNLFNQNGFNQNGLNQNLINQNGFNQNGVIKNELNQNEANQNRLNQQFQLQNNCMPNIKIKSNQFQTPPQKEDLSLISPHQKIKEYLQFLLNDNGDIIKDDIKNLIQYLIQNSTFSIDQLKQLITIYKNQIQKPDLCENKTLIFFYCILCILNNTTPLNIQSFDDKKLREEIKRLYDNLNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQF2</accession>
    <name>A0A8S1KQF2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0260175</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQF2"/>
    <dbReference type="OMA" id="VHRKTCM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="6">
        <location>
            <begin position="121"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="201"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8057700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="259" mass="29860" checksum="BE7E8F40E61B1352" modified="2022-10-12" version="1">MPKAPKTSKAKKVQKKVADRKKNPLFVKDAKNFRIGNDVQPKRDLSRYVRWPRYILLHRQKKILLQRIKVPAAIHQFSKTLDKNQSSKVYSLLKKYAPETKTEKKQRLVKAAESKAQNQKTDSKKVTVLKFGLNHVTTLVETKKAKLVLIAYDVDPIELVVWLPQLCRRQEVPFAFVKNKARLGALVHQKTATCVALTEVRKEDQAEFDNLARDLRQHYNENHELLRTIGGGQVGIKSKHQQEALKKALELEELKKTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KT87</accession>
    <name>A0A8S1KT87_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="WDIKYKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1422" mass="165362" checksum="5A014CA107662082" modified="2022-10-12" version="1">MGRIIKKLEYGLQVGRENYQAMLAVNTQMRGKNKVNGKKLSKIFVVRLKFMSWERTWKQIYEDQSIGEGDYNKQGQKKGSWLEVSYSFNDNSQVIYKGEYKCGKKIGKWEILYRFSEIFELIGGGFYEELGANEIKIGNWIELSDQFNDDAQVIQKGEYKYGKKIGKWDILYRFQEQFELIGGGFYEELEASEIKIGNWIELSDQFNDDAQVIQKGEYKYGKKIGKWDILYRFQEKFELIGGGFYEELEASEIKIGNWIELSDQFNDDSQVIYKGEYQNGRKFGKWEIFFRKDDEKPFKLQGGGSYADSFEDNISIKIRRWVELNDQFKWNLQIINIGEYKNGKKYGQWDFWFQQNQGDKIKEKIGGGLYNDQAEGSSLKDGRWIEMSDSFKRNSQVIYDGQYQCGLKVGLWKILKKNKSIFNLWYLIYLLKQLVVVLIMIYMEKKFLIVDKILTSFILENLKMEKKLEIGIHYIGKIKMDHLKICKQQENIKNAFRGGGTYKYLMDEDSIKVGIWIELSEQFEENLQILYHGEYRNGKKVGRWNILQRKNSKRNYDLIGGGYYDQDQSTKIGKWVEICNEFSDQQIIYQGEYKNGKKISIWDLFCQRKLLNFNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKRNYDLIGGGYYDQDQSTKIGKWVEICNEFSDQQIIYQGEYKNGKKISIWDLFCQRKLLGFGSYDYSSEGISMKIGKWIEQCNQHKKDSGITFNGSYRKGNKFGRWDIWYQKNYGNYHKELIGGGLYNDQLGEDSIKIGRWIELNDQFRDYSQIIFDGEYINGKKVGRWNILYKQDDKQLFELIGGGLYDQSNSIKIGRWVELNDTFFDYQQIIYEGDYFNDKKFGRWNIYYRYQDEFEQVGGGYYDNKDGIQEKVGRWIELNEAFNNLRQVIYEGEYKNGKKIGRWDILQRIDGIKKDQQIGGGLYDEKSSIKVGKWVELSDKRNSQVIYEGEYKNGKKVGRWDIFQRQDDKAMFQQIGGGFYAQRGSIKIGRWIELSNKRNFQVLQKGEYKNGKKVGQWIEVMINGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KV16</accession>
    <name>A0A8S1KV16_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Plectin/eS10 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0250310</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KV16"/>
    <dbReference type="OMA" id="ERTKIHR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005326">
        <property type="entry name" value="Plectin_eS10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037447">
        <property type="entry name" value="Ribosomal_eS10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03501">
        <property type="entry name" value="S10_plectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Plectin/eS10 N-terminal" evidence="6">
        <location>
            <begin position="4"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="99"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8057182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="138" mass="16265" checksum="B74FACB2FA324F1E" modified="2022-10-12" version="1">MVQVKRQDRRKVYEYLLMEGVIVIKKDMALPLHQETGVKNLEVWMLLRSLRDKKLVDLVFSWQYYYYYLKAEGVKYVRDKLGIVEDVIPATFKKADKKFEDDVPEQRRGPRGNKPFGRGGNRGPRRAEEGTETAQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVK7</accession>
    <name>A0A8S1KVK7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVK7"/>
    <dbReference type="OMA" id="YHTILIL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="425"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="550"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="68096" checksum="33AC0FE081FF3020" modified="2022-10-12" version="1">MEEINTQKQEDSPAPSGSLIVNIEKMQRDSVTEDKIQPEEDIELQALFNKLNSTIDETPFHETKCQKQKQDLSYKAEFIMTHFIKFYIYQLMFYIFGPLIFFFLLNKPALMFNLGFWSWRQDVVFQYIQWIGHIFCLLMYFYFQSLSSLEIGLLWFSLLTRSIIVAAKFSTLNEERVELYERNRLSKKTIYFDVVLFDWSIQSKKIKYLEIAKAAKRHDFDTQFFYFDFLVEPMKETQKALLDDQETLLNLPHDGTYSGINLISYFIDSYQQLNTRKNQLSLALLISFIVVITPKALAYERYLQNPVEIFLNIVCGIFQIIQFNCIFLYLLISLEDMKRKIFLLDQVYYLISTKRVRCKEFKLTPTIDINCPRTIEAWSMLRSITFDYGASYHIRNQIYHTILILCCVASLVFAFQIILDYFQLDYYYLITLGIVFLIFLIFISLYLLSAATINQFFEDFKTQIENLKFICQDVRRMRKQYFEDNNSEPQNFVHKSFVNHLKAQHNNNNLIIYQRIDNLIDSLDNAQRHIEYDSRNYPLKLYGIKITYEILQNLVVGLFTVISFVVQQRFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0J4</accession>
    <name>A0A8S1L0J4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PAZ and PIWI domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0310092</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the argonaute family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L0J4"/>
    <dbReference type="OMA" id="RIIPMEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02845">
        <property type="entry name" value="PAZ_piwi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04658">
        <property type="entry name" value="Piwi_piwi-like_Euk"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAZ" evidence="2">
        <location>
            <begin position="191"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="3">
        <location>
            <begin position="457"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="3">
        <location>
            <begin position="867"/>
            <end position="1171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8061007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1194" mass="136494" checksum="FDF02C1D9ECF5BCB" modified="2022-10-12" version="1">MIERGQTVYTNFYQIQINPNSHIYRYQVIYTSLFSLLQLSFQPFLSPEQQISKWREIFKIAKSGLQENLKVFITNNQILYSPIRAQTMGLLLGVYEEEGTSHSISIEEKAVLKPGEPEYTGLIGRFFKMLLKQQKLLQIGRKYFNTKNLINFDQFGLKVLPGVTCSLIKQEEQGKYYINIDSSFKMLRSTTMYEELRNSRDFSQLEGAIVMTVYNYKFYKVNKVSREMNPKSEFENLKGEKMSYMQYYQDKYKIAIKDVTQPLIEVLEKSRKKQEEKIIYLIPELCVMTGLSNEMRNNFQTMKQLSTVTKPRGVDRVKQADQFIQCFHNKESEELIKKWNIQLEPKCLQIQSSKIKPGNIMMGNNTAINIETGNLDRDTQTAMLRGVGLENWGILYSDRDSRQAEDFMNCLRESIEYCKFQCKAPRTFVLHSNRIEDWIKQIDLIVQQSQGPQKVTLLLLILNGPKKNAPLYTDIKRYLINDCPIASQVILSSTLNQPKGKVKTICNKLLVQICAKVGGTPWGVSELPFTDQPTMICGMDVYHSTGKAKKSMLSFVSTEDEFFSKYMTQSIEMETGVEFSFSLCPVLVKSLQSFCGDRNGPLPSRIIIFRDGVSNSQAKTVIETEVAQFRQAIEQVKTEKNSDKPIKLIVLSVNKKVGAKFYAGERNLDNPPQGTLIDTEISNGKDDYYLISQRTTQGTVQPTHYHVLVNDLFDEPNILKKLQALSYKLCYMYYNFSGAIKSEELIKKWNIQLEPKCLQIQSSKIKPGNIMMGNNTAINIETGNLDRDTQTAMLRGVGLENWGILYSDRDSRQAEDFMSCLRESIEYCKFQCKAPRTFVLHSNRIEDWIKQIDLIVQQSQGPQKVTLLLLILNGPKKNAPLYTDIKRYLINDCPIASQVILSSTLNQPKGKVKTICNKLLVQICAKVGGTPWGVSELPFTDQPTMICGMDVYHSTGKAKKSMLSFVSTEDEFFSKYMTQSIEMETGVEFSFSLCPVLVKSLQSFCGDRNGPLPSRIIIFRDGVSNSQAKTVIETEVAQFRQAIEQVKTEKNSDKPIKLIVLSVNKKVGAKFYAGERNLDNPPQGTLIDTEISNGKDDYYLISQRTTQGTVQPTHYHVLVNDLFDEPNILKKLQALSYKLCYMYYNFSGAIKIPAPIQYAHVCSNFIGDRFDPRKPQSLIKPNPILNQRRSLFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0R4</accession>
    <name>A0A8S1L0R4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L0R4"/>
    <dbReference type="OMA" id="QGRFHIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="590" mass="69666" checksum="EC3FFF5339FF7C25" modified="2022-10-12" version="1">MENYNFSALSISIQTDTQSSQSDQQLKFSSPLKSEKNNFVDLDNQKENNDSANQSQVEVSIQKDCQEKVAQTSEQKSNSICGSQLKQTKASQNKKRLVKRCQTTILIGQSIHKHNSQKADKYFMKKNNQIIETSFEKLKVKRSNFSGIFLTNLRQQQKPIQVRYFRSTITKVLVIEQDMLAAEFIVLALTQFQNDSKLDQSLYEFPNYSLAYQIEGQDDQQDLDEFSYRRPLQRYKSFGSLEDKMKSRRESLDEINYDQKPVSQAEISLLADDIDDKIIQIDLESPMRYYKSVDIVPILDQKQLDIDLQMVKILHQQLPNHILILIEEPISQGRFHIKIRPNQLVEELFIELSRKANKKYRSTDYQLILKYPCIHMSKEQINLKMPINKLPIHYLVLTSKFQNEIIRSLRQCNDSFTVNINKDTIFSKSANKSYRFLQEEGKMSDSVDKVRNQYSYQEYQLLKIDKQFKNNVILGIDYFDLYYTYTQERQRQFSLGKLCKSISQILFEEPLIQKDYRRLPLNKIQNVELTKDFNLIIVYEKSQNYRKQLHFGIDLNQEKSKIDLQQKSIMNACNKLEYLVDLYIKYKQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1D1</accession>
    <name>A0A8S1L1D1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0310278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1D1"/>
    <dbReference type="OMA" id="MFTHIAS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="27120" checksum="11CB318DF516B191" modified="2022-10-12" version="1">MGFGTNPYQNENHEDKSQYAITGRPQLNDVHSIYVKGNQPWWRRWWATRDMFQEHHWEVLTCAKYAYKGALTAILVGATYQFKQLSVNALAINKGRVFASKNAFTGPAFLALKQASRHGTLGALFGVAYFFWFYQLVCSRGHQHTFNGYLFGHALYGAALASFFSIRRSGVGALVGGIIGWIYYNTAHGNLQIGNKAEEGINFEFKGLTPEQREAIRQRDLISHLSRTPEFKLNLFNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1T9</accession>
    <name>A0A8S1L1T9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0320161</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1T9"/>
    <dbReference type="OMA" id="PKREWNY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="172" mass="20554" checksum="FE5613C915A79990" modified="2022-10-12" version="1">MQELSNAIIEFFDLVIEKYITNFANPKREWNYFKSLLSSSSIDLNRIQSYLKVLKNSADQQKPTLRIDPIKMDNYMSYVSPLRDHNRPFEQQFNHKFENVSLNQYLSQPRLEQNPVDQCVLFKKITSKIVNLMDSNNIQLALTKEIQIQIQRPIERKLSDIERYKYIQQQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2W1</accession>
    <name>A0A8S1L2W1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0310225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2W1"/>
    <dbReference type="OMA" id="TEFEWII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="110"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="423" mass="49376" checksum="A6DDA3CBB0DCA80C" modified="2022-10-12" version="1">MQFKSKQNDQQILHFTVIRKHFFLDRIYYLKLEKDFIIMSSETDFKKPKYYLSLSPDTEFEWIIRNQQLVGFLFPYRNKIKEFYGDKNHLTQLKLQLGKIILFKSLIVHYKFLLYLDQGAFGSVSLQSNYITENKFAIKALTASNKQLQKQIMNEITTLRSLDHPNILKLYEVFKTNSIYYLVTEYIDGKNLKQLYNEIHQMQDNSILCLVKQLFEGLNFIHKNNIIHRDIKSANLIIQKDGLLKIIDFGLACFDGDQLKDYPKCGTPGYCAPEILQNIGNKNQYDSKVDVFSAGCVLYKLLTSKSLFEAETSKQVLKKNKNCLFHIKEQGRLFDLTKILVKQNPLDRPTSFQTLQLIQKMIDDESFDVNIWYRNTFNCNQSTSSLLSNTPKNVNLSKSNQKTLSTPNSSIQFFSNTINKIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L337</accession>
    <name>A0A8S1L337_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0320222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L337"/>
    <dbReference type="OMA" id="HKANMSP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="1">
        <location>
            <begin position="114"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="235" mass="27295" checksum="1701AA8F80508C35" modified="2022-10-12" version="1">MNFMKETLELLGYENIKCSIPNTLLDNFTAFFTAQQQNPLTNESPQNFENGLIFMSTISQSNTPQISAQQLRSVQSKGSKGKIRTFFPSKWPVHKANMSPFSFSKYSHFQSLTSRYWYYLDNSNTVQGPFSCIEMDNWYRKNLLNHDLLISYKSHDLTSFVKIQDILKDSENTKCDKILRQLYKRAYSTKRIKDQNYSNSPISKASTEISISNNNSCQKSRQPIWGVDNDYFLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L490</accession>
    <name>A0A8S1L490_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0300107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L490"/>
    <dbReference type="OMA" id="SMEFYEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="331" mass="39273" checksum="9FE3BC9033D122C2" modified="2022-10-12" version="1">MAYILYNKIKNLILRQPIKTKQMSTAIVLIKFLVSRSREEFQLLIQSASTSVIGKMKQQLEKKTDDTSNDSKVEKTEPEETKPKLLLRKKVMIVRENTQQQMKQPEIINRSVSVTDKLVMYSQPQESNLNPEKELKYQLRLQQRKLRKDLKNIYQPMIDLDNDHMWCSHAVLKKKFMKYPEKYFLKRGAFYIWKYLNNNNSDIRITIMNFAYRRAKLVIVDKILQEQIQELHKQYNITGQIPRQSLPLLVSCQQTPKIMKPQIRLQQQLQVPNISQLPMLRSQHQQIKSMEFYEVTVKDQKAHKSNKSLNLTQEQAKIQQKIKLQSSKDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4E9</accession>
    <name>A0A8S1L4E9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0330325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4E9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8062738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="239" mass="28350" checksum="F01004D5EAED7974" modified="2022-10-12" version="1">MQQQRYDSVRKNKLEVNLSQPLQLQYSSQPKQIPRKFQIAQIGKDIAAIIIPAFIDMINQETDVYYLFDSFHKFKDMNMSQKNKQHTLDWWKATGSIQSWAGERNIQLLSESHIKQSSHFLDSYNSRGFAIIENLKRQIGELTEEQKFKQNSRSIGQVSIKLFDEQDLYVEMSKSLQFIKFKINFINVIWVFVCTVFQENFQKERAQSIKYMWQSGTEKMATFQILLQNLYSKQSKMKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5T9</accession>
    <name>A0A8S1L5T9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0340002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8062809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5T9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035868604" evidence="1">
        <location>
            <begin position="19"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="180" mass="20981" checksum="CDE10D29AECBE24A" modified="2022-10-12" version="1">MLKFCVILVIFAISLNQCQRNFRYCSRNNKIKALTRGYYKNVMSYDASPLLTSRKQNLFVGVGKEMGILLRNEIYSADSNNRGTYMSLGKSIIIELLQVYQLNTLKLMLRNGNVRTYDFIVYASDSNKEIKIFDSITYYIEVVTIKFPNQFVKYFRIYNRGGNSDAAFIHIIKAEAYFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L778</accession>
    <name>A0A8S1L778_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310287</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L778"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="120" mass="14253" checksum="FC5899A78117A150" modified="2022-10-12" version="1">MNLFKEFFTQIARIKSKNSISTIDTLIAMYSKCFEYYDQQQDPIKYYFLEKIQYTLSQDHTIEHITRNSNRTQTTRVSISKLDPELLNTSIRSKQTNNPQNQFSISTQSRHTQLTIKYIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L7K5</accession>
    <name>A0A8S1L7K5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0320027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L7K5"/>
    <dbReference type="OMA" id="NMGEAVI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000068">
        <property type="entry name" value="TBC domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000028">
        <property type="entry name" value="TBC domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000007">
        <property type="entry name" value="TBC1 domain family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF26">
        <property type="entry name" value="LD44506P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="73"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="385" mass="46155" checksum="44FA7F52A0B83693" modified="2022-10-12" version="1">MSFLKKLFTKPTKEKETQQVQHNINQIYNTQPFQRKSNPIYNYNCIYRIEQFEIILEQQIIDLEQLKQLTWRGAPQVYRAKVWKLILKYIPPNHMSQTNVIQKKRQDYFQYIKNYYEQIHERDESEEKIIKIISNDVLRTQPDFQLFRDPKIQNLFKRLLFIWSQRHPMSGYVQGINDIASPLIAVFLNEYVRVDYNKFDLPSDFHKLPDDILLDIEADIYWCLCKIIENIQDYFIHNQPGVSLAYEKIKNILNTIDPTILAYFQQQQIDFNHFAFRWVICLLVREFPLYLAIRLMDTYLSEGDNMANLHIYTVSNMILKCGPEIKNKNMGEAVIFLQNLPTKDWTELDIEMLLQEAYVFKEYMTNKQNLNQSMTPTQSNRISNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L939</accession>
    <name>A0A8S1L939_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">cGMP-dependent protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPRIM_AZ9-3.1.T0350275</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8064057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L939"/>
    <dbReference type="OMA" id="DNQIHCD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000068">
        <property type="entry name" value="cGMP-dependent protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000123">
        <property type="entry name" value="cGMP-dependent protein kinase 14-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000089">
        <property type="entry name" value="cGMP-dependent protein kinase 5-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0140">cGMP</keyword>
    <keyword evidence="12" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="73"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="191"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="311"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8064057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="520" mass="59664" checksum="6E14E2C87ED15F47" modified="2022-10-12" version="1">MDPRSQNNSDNQIHCDGEEDQRSGGDENDKLQNFKHNINEGGEVIHENVKRIDMKKTLNDTTFILTCLKNHFVFYNLSEAELENIVNKMFYCEAAAGTYIFKQQDHATCFFILQRGSLEVIVNEKAKRELKTGDGFGELALLYNAPRSASVKCFENCNLWGIDRNTFRRAVEEMITKEYEENRKFMEVVRFFHNLTNEQKDAIAAVLIVQKFYKNQIIVNEGDPGSSFYIIKEGTVSVLKGNKEVRKLYKGDSFGEQALYYNTVRQMTVRAEDDVKCLALGRDSLTKILGDQVHVVTFRNLQKWAFEKNALLSKLTKAQIDKVLDVMKISSCKAGDVILKKGTQANQKIIVIIEGSLKKSKSGITVATKAQAWGEEYFLQTNKAKILDDDIVMETDGVIAEITADNFIDCIGGELEEVIKKNEKILEKKLQKSDQTKKKEAQNIKKSELLHIKTIAYGQFGPVYLVKAKYNQQLYVLKAFNKNQINEQTLEKQYLMLFLCTYNKRNKYQKQLIFHLLYPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCZ9</accession>
    <name>A0A8S1LCZ9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DM2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0360029</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCZ9"/>
    <dbReference type="OMA" id="VMDSKHH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10568">
        <property type="entry name" value="SWIB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019835">
        <property type="entry name" value="SWIB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00151">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DM2" evidence="2">
        <location>
            <begin position="194"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8064245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="426" mass="51114" checksum="7F7C963CE7D558D6" modified="2022-10-12" version="1">MASQQQQQSTQQQPSQQQQQQQQKQPIRQPDVKTQLKQLYDKSSIQFDDETLQLCPQISILNNLVKYENRIDQMIKNKRVDLQESFIQPGQYLKKTLRIIVYSELVCDEWNLFIKGQVLSEDKKPFSYFIRQLEVQFDKTYYPSQNVIQWSRNHIQQQQQQQQSQQQQQQETSGFHIKRKGPSCDVIISISLQTYPQKYKLHKTLQLLLGIKEGTRSQILYCFWEYVKLNNLTDKENKDYIIADEQLKQLFGQERIPLSNLNMLLKMFIENPEPIIIKHHLGVSNYIGFDVVVEQEMSYQPELMPFLAQKVVTEDNSEKKQQRAEHPFIQLNQKIKGFEKQIQKYIEQSKSHKLKRDAYYQYQKNPSLFLENLFLQQNSYLELMQNDDEMRNDDPKNMKFLMKNQELVERQIRKYLEQQQQPQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LE68</accession>
    <name>A0A8S1LE68_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0350168</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LE68"/>
    <dbReference type="OMA" id="SGCAVRE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_eL34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_eL34_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10759">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8063843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12282" checksum="3C67572FB96088F8" modified="2022-10-12" version="1">MGVRVTYRRRTSYNTRSNKIRKVKTPGGRVVVQYPNKKTSASTCADSNLNIVLNGLKRIRPTKLKQLSRRQRTVSRPYGGVLSAGALKNRIIRAFLVEEVKIVKQIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFJ3</accession>
    <name>A0A8S1LFJ3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0320064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFJ3"/>
    <dbReference type="OMA" id="MTRKTSM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="131" mass="15477" checksum="05294786E84C33D4" modified="2022-10-12" version="1">MRKSQKMPSQQQMKNIITPTVTQQITYSPELSPINKPQKQMTRKTSMIELYRVESESIRIRPQRVLQIIKDAKQQLFSQQNPRNEKKLKPRVETSEGQNRGLDFTFAQNFSNKQIIMKEFSSNGLRKKFFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LNZ7</accession>
    <name>A0A8S1LNZ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0420006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LNZ7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8068145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="163" mass="18861" checksum="9F6741686737E40A" modified="2022-10-12" version="1">MQVEFQVVYKFILLEYPSVGKTAFNINSFNEQYQPTIGVDFHSIKFDLNREQRFISLTSISIKGVVIIGDITNRQFFNDLSKWQEKVKNNTNSYVPKLLIGIKSDLTGKEKSFLKKDSNWPILQVCQTSAKDGINIDQAMKNLLNEALAQYEKYKKEDHSKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LVU5</accession>
    <name>A0A8S1LVU5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0440202</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LVU5"/>
    <dbReference type="OMA" id="CWISWIP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039309">
        <property type="entry name" value="BT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31585">
        <property type="entry name" value="FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31585:SF0">
        <property type="entry name" value="FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03092">
        <property type="entry name" value="BT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8069731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="228" mass="26564" checksum="85D138A3A68002CF" modified="2022-10-12" version="1">MKQLRANLQKFILDPVIYRPLLFIFFFMATPSPATSMFYFYINQLKLNDEFFITLRYTYSFCTIMAVTVYYLFRRYPFGQFLSVSNMLYFFASFLSLLLVTRINIDYNIPDYQFCMWDNILMQTLQEINTLPVLVLGAKMCPKNLEGTVYSLLTGTMNLGNTISKQIGIVLTFYLGITNQNFTHLWLLILICTVGSLLPMPFIGTVNEEEVDQKKKSLAKDEIHIKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LVY4</accession>
    <name>A0A8S1LVY4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPRIM_AZ9-3.1.T0440230</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LVY4"/>
    <dbReference type="OMA" id="YMITELC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000673">
        <property type="entry name" value="CAMK family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000557">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000974">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="45"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="342"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="379"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="415"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="453"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8069771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="482" mass="55506" checksum="69B4A2AF692FEBAB" modified="2022-10-12" version="1">MGCASSDSASANNPRKLEQQKEFTGEKISSEIFVDLKDGNIYEEYEVRSTLGEGAFGCVKLVAHRKTKMPYAMKQIKKQGLIKEDQKILFSEMDILRLIDHPNIVKLHKLFQDNIYYYMITELCQGGELFDKLASEKNFTEKKAAEIMKQVLSAVTYCHERKIIHRDLKLENILLETKSANSNIKVIDFGTSRKVQEDEKLKLKIGTLYYMAPEVFQGQYDLKVDVWSVGVILYILLCGYPPFNGDDTTIKRKIQKGTFEFNDTEWRSISQEAKDLITKMLKFDPQQRITAQQALQDPWIQSKAPNTPVQANALNNLKDFYSTSKLKNAIQLFIVTQVTTYQEKEEQLKQFKAMDIDGNGTISPDELKKHYSKLYGQEQAEKLVQEIMKQVDINQSGQIDFNEFLVAAVNKEKILSQEKLKQVFQMFDKNGDGKIQRAELQYIMSGIKIDDGQWKNILEECDKDNDGEISLDELINLMQKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZQ5</accession>
    <name>A0A8S1LZQ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460287</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8071081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZQ5"/>
    <dbReference type="OMA" id="CKEDNCL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="239" mass="28574" checksum="FED39FABF79FEA3E" modified="2022-10-12" version="1">MNQTTPKSSGINYAKLRKQKSKETTIPKSMSNNLKFDIQQNHQYGILKHRSTSQHNIHEHVQSQQQIQLFEQYFNQSKHLLPFIEFVTKQIQYHTQPIQINANIFKNGQRRWDDEKRRILNELNQCKEDNCLLQSEIHELKRQKNTLSKQCENLQVIIVQERLGKQQKHHHHFYKFQELTKNIHVPTQETISSLTDERFESNETSLDSSLSNDDHHHPNQLFELLKTGKHRYQFSKKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M2K9</accession>
    <name>A0A8S1M2K9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0520092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M2K9"/>
    <dbReference type="OMA" id="VETQEHW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17986" checksum="C2C8E9D5053F30B8" modified="2022-10-12" version="1">MMQQRDFYGNDYIYKHIKQVSLQEFTDQLFNPVRHQKQMVLFTQNEQKEQQELYKYYVYLGGGVFAVSALSVIFYKKIPFLNRIEKRWARILTKALLLFGPLQVQSIFGQRAQYELLDKQFIQYHRQLQDYMFSGDIRKLKQDIQISPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4T4</accession>
    <name>A0A8S1M4T4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0470275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="1">
        <location>
            <begin position="183"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="351" mass="40400" checksum="724E1A9A705FCD47" modified="2022-10-12" version="1">MFNFLEVNQDKVSQGKEITQKKFVLQKQLMKYKRNLLTLSVIDFIRFSKKQKRLAQKLLXKQKKYELNGNVIYRIIRQYGVQKVLLSSQELFKGQVNPGDYKFFFSLPTKENMSSSFFYTSQDGLKSGKCSYKITLQIENLDECLNILKESAEVYINARVQSSVEIQRQSEANIISYFCLNGGIAYLSIKLNKSNFTPGEQAIIEYQIDNSFSSKNINRVEIRLINKLLFIDDDQVDRLVLNQVVFSKILSGVNQGQRLEAQIAQFEIPKNLKASVRNQTISNQYFLQIEALIDQLLISLSNPILCQIPINIQESRFQQVVNLDGWNFVPILNASVNAFSNLTVQQSFIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M6Q6</accession>
    <name>A0A8S1M6Q6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0510077</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M6Q6"/>
    <dbReference type="OMA" id="PIPSRWP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="477"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8073491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="610" mass="72155" checksum="E1C75E6DC3141115" modified="2022-10-12" version="1">MQNIELFSNDQDEAILSERNEQEQFQSESSKEDDNSSDSIQNEEEQEMNPKESQTSTFPEQQIIKDETKKKDIKETCLEKIKSYIGFGHSDLFQNERIGSIFFLPIPSRWPLKIQVTIAQIIIAFTSLSLFVGVQQIGENILFNTVQQWTKNNVSQLLQKQICEITIDFVQILIKTQENDIQLINVTLSLIQSESLRYKDILYKIDNQIPNSSKNYVTNGGIIDQYTLNIATYSLLKDQQISENKIRTYLSINNIMYYNYQTLGSNFIWIFDDGFLMQYPGVNVTDIKKFNEYRLQMYNSRRNLSHIPLEYTYDPVLNQILQREVLPIMDINNKQIGLIFCERKPFLLYILFYQIAINNQYNYTLFLFSQENDILYYEEQEYKINIEEFQQLLKTMNTDDSKLKKINISQFLFESTSVFSYFELYQGDLSNQEFILAYCNYQGLYFGIFQPTFLANEEVEFQKIKYYQKIDSFNKQLIPIALSIPIIYIIFCVFYMVRYIKPLQRITEYADQLLQKSQNFDEKQFEKQFNNSKGDDLIQQLTSLFAKLMGNLNKSKQLKKQEIIDFYNKQTYPKNQKSRDVTPIINQVKKIKLDQIVRDPGVLQFPNLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEU7</accession>
    <name>A0A8S1MEU7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0550191</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8076015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="LIYYCFC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="432"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="859"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1090"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1180"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1260"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1094"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1279"/>
            <end position="1290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8076015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1368" mass="161922" checksum="1C0D86F3E22387CB" modified="2022-10-12" version="1">MLVQPHPNIQQGSCSRSMSISMDDSIHTYIQPIVQKMTVVNKHQLQVRKPSDGAQEMIQSPPTVTPHIASDRPPQCEIKDLLLINEEPSNKEHSIQITDRYKKTHKATQNNLSQQSILIDEKPLHSYSDVQWSGLISLNTINSNSSFQHNLIQDKAADFNPNNSNGISFHPKKQNLFYHFCNATYCMYICFFITYMLIHLQDSFQKQYTYQILLIVIQIIYIFNQILFEQYDNGDIINTFEQLTQLYFRKQSIFDLITIIPVIFALSSEQCHFIQILHIIRLWKLRQLIKELELIFGKVIKFTYYIMEFHFIQFTALSILQYNHNKQINFYNEFLVLLYKSDNSNAITYIIRMLILIYYCFCIFRLTFQDNLELLNKLQLTCSTKQQIKAYMNKLNSQKSIDMNFLSQLPQTFVDDVKFQRYYHLLSKIPIFKSSFSENTLKQICSLIQEYTYIPNQIVLMQQTSNQHLFIILSGDVQISQKKAGIDFKIKVLGKDSIFNNQAFFKNAYSNINATSIGYSQIAQLDLDLFLEFIKSHYQERQKYKMMVDQIVLYEIYSLCQLSCYVCGKFHDIDECDHVHYVPKKSILVQYIQQNDQQIRRNYFRSKARRKQKRLKSIILKIEEAALQYQQQNIESDITDDNKKYLDEVISIQQAYMGEASHRYTDRQNSFQQSFRKQSIFDLITIIPVIFALSSEQCHFIQILHIIRLWKLRQLIKELELIFGKVIKFTYYIMEFHFIQFTALSILQYNHNKQINFYNEFLVLLYKSDNSNAITYIIRMLILIYYCFCIFRLTFQDNLELLNKLQLTCSTKQQIKAYMNKLNSQKSIDMNFLSQLPQTFVDDVKFQRYYHLLSKIPIFKSSFSENTLKQICSLIQEYTYIPNQIVLMQQTSNQHLFIILSGDVQISQKKAGIDFKIKVLGKDSIFNNQAFFKNAYSNINATSIGYSQIAQLDLDLFLEFIKSHYQERQKYKMMVDQIVLYEIYSLCQLSCYVCGKFHDIDECDHVHYVPKKSILVQYIQQNDQQIRRNYFRSKARRKQKRLKSIILKIEEAALQYQQQNIESDITDDNKKYLDEVISIQQAYMGEASHRYTDRQNSFQQSQQTPRRGSLYSMRESLHSLQQQQQQQQQQQQQQQQQQQQQYQIIDIPNISPVQYVQQPQSGMSHSSYGQLFKDVSVNRKNINGSSDKNSSGNVFSLPYSSNGSKNTPNNIISIQEKEELINIQMKRLKLQDSVFYHQKRIQNQMDNCSQNTLSKYQKYHTTQPQQQQQQKIRSDRKSNSCISESEQGEYQLQQQQDLQPSRYGIQSSKFTGETAKQFMKNPLEMPNLEFYQSFENSKQFSDYFPQYNIDQAIQCYINYQQVRSSAYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MR42</accession>
    <name>A0A8S1MR42_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0620093</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the prohibitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MR42"/>
    <dbReference type="OMA" id="DTHMPPP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000001">
        <property type="entry name" value="Prohibitin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF0">
        <property type="entry name" value="PROHIBITIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="3">
        <location>
            <begin position="25"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8079573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="274" mass="30671" checksum="A6C604D60056DB2B" modified="2022-10-12" version="1">MERAANLILTAGSLLVGGGILFQSFFYTVDGGQRGLIFDRFQGVKETVYGEGMHFFIPVIQSPIVAEVRLQPKTVASHTGTKDLQTVDIAIRMLHKPIESYLPEIYKTIGLNYEEKILPSIANEVLKAVVAQYDADQLIKMREKISQEIKEGLIERAKEFKIVLDDVSITHLGFMKEYAQAIEAKQVAQQLAERQKFIVLRDEEEKNAKIILSEGESEAARLINDAVKQYGTAQIEIKKLETAKHIAEQLAKSPNITWIPTGNGVSNLLNLKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTA7</accession>
    <name>A0A8S1MTA7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0630184</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTA7"/>
    <dbReference type="OMA" id="QSANNKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000016">
        <property type="entry name" value="PIK1p Phosphatidylinositol 4-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF22">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="5">
        <location>
            <begin position="395"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8080283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="669" mass="79200" checksum="C8A603B19544DF02" modified="2022-10-12" version="1">MNQSNQISIDESFRKIICKDVQESTLMEFLERLNQLNYDETSKYLSGLVYLFTRKRWPQLGEYLLKMCAEHIAFYEIIKWSYEAYTYKERQKAENSVAVFDQKIEEAMVNQISENQFQEKESRSNYVNSIQRFVTCLTVLSLHLKNVPLNERQLYLRRNLIKGNRALESFRRNNPGIKYCKGIILPFKGRSQMVVRIIDTEAQSFNTKKRVPYKLVVETVDMDEYEEREQQLVTLQKQLSIDSQAFLEFDVMHQFDYTVFEDFLQENKDKLARQQIINQNKKRFDQQFPPLKKELIIRRRSCIDQIQSYTNKQLYEKDFKDLFKQEDKLKHSDKELDNITFLDQIANEYPNKKGTIEKIQKQLNDSKIIIQQQEIQQLQSPSQNDLKNGPFSEQWEDKRQIIQSNSPYGQFKSYNLRSLLIKGGDDLRQEQMMMQIIKVSEDILTQVGLYVKSYDIIITSVDSGILEFCNDTMSMDQLKKSMPSMTLKEIYNSIFGDNFEEAQLNFILSLTSYSLLQYLFQIKDRHNGNILIDNQGHIIHIDFGFILTIAPGGFKFESAPFKLTKEYIELLDGQESCMFQLFKSKLLLGYLELRKNVDKFAFILEAMKIDNELPCLEKFDMKVFKDRFKLELNEQQLEEHVHKLVTQSANNKYTKYYDDFQKLTNGIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MU14</accession>
    <name>A0A8S1MU14_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0710016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MU14"/>
    <dbReference type="OMA" id="SHQMRNK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="341" mass="40030" checksum="37DEF1CE15186C9B" modified="2022-10-12" version="1">MGNILMKCKDCFQFHKRKQTSQIQTHQLVNLSNNPEEETQGKINAQQISFTQDPTQSNERTISQIDEKNENEITLETISNQQLSALRSSQMNSNQNQNEQQQFMINEDCKTNPSLDSPYPLSFKEGELITLSSINNINQQEFNYYAQEAMKQFMEKIEIENNESGEYLVNGQELDLYASHQMRNKKMFLIIKFSFDLNTDITTFLNWSNDKKLFDFDLINTYNLQKLNEEEQIGEIILNRQGMIKQQYFTYLKTIKKIDEVYYIAFQSIRFEDKFEEYKKEFQEGHLTLGGLKISQKNKSSLIIKGYIDVDLGIKVGFHLTLKTIRDKVVTSITKLRELFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MVL1</accession>
    <name>A0A8S1MVL1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0720038</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MVL1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000009">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000109">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="13">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="14">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8084388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="49792" checksum="819ADA13CF39E568" modified="2022-10-12" version="1">MREIIQLQIGQCGNQIGTKFWEEIAQEHNIDENGQLIEQEHEIQQYLDVYFNEVNGNRYIPRNVLIDLEPGTLDSIRASRYGKLFRPDNFIFSQGGAGNNFAIGHYTNGTEILNEVLDVIRHQVENSDCLQGFQITHSLGGGTGSGFGTLLLSKLQEDYCDRIFQAYSVFPSIKVSDAVVEYYNTAVAVNHLIENCSQCMSIDNEALYNICTNTLKLKNTCYGDLNHIISASMSGTTCSLRFPGQLNSNLRKLSVNLVPFPRLHFFTTSFAPLTSRQSLQYRSLKISEVSDQMFDPQNQMCAINCKQGRYLSGACIYRGQFSSSEVEKQIIQFQKKNPQNQVQWLPNNMKVSQCNVSQKGVVNSGTFIGNTTAINEMFRRIHNNFQAMFKRKAFLHWYTNEGMDELEFTEAGANIEDLISEYLQYSEGNDDDDEEQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXK8</accession>
    <name>A0A8S1MXK8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0730151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8085108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXK8"/>
    <dbReference type="OMA" id="VMYMVLM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="67"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="211" mass="25135" checksum="674C79394A77EC56" modified="2022-10-12" version="1">MTKEEIKEAVEAFLKDKLDQIWQKIINLEQRFQIFERALTQQQSDISKNNNNQNTQILHLGKKFIDLQKQLQAAQEAANQVVEMTEQKIQETQKIKKNQRKKLEELDKNQTIQQQINSGFSQLIQSQQQQQQQQQQQQQQQQQQQQQQQQKQQQYQFQTLQVIPEGMVFRTRNGTKYHLINCENVQKYQTMMITIQEAREQNLTGCLNCKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZI1</accession>
    <name>A0A8S1MZI1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0720237</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMEM43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZI1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012430">
        <property type="entry name" value="TMEM43_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13416">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13416:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07787">
        <property type="entry name" value="TMEM43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035826331" description="Transmembrane protein" evidence="11">
        <location>
            <begin position="27"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="394"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8084742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="51203" checksum="583CE70C76C87225" modified="2022-10-12" version="1">MRRFIQSNHRLIELILLSILIPQCNSSNNLRRHIQWHQIEHEPTSFSQVENTICYIFGFISMILALSILCHNERKNAITEYRLEEAKKLCTSVNSLEVNPNTNNQLIHINGLSQTSDLIVDAAFGLTLKDCVKLVRTVEMYIWVVTYTENEDGTTSASFSQEWSTDFSENCGSCKNDKTKLIVEQETQINKNVHIGAYLMSKSLALQTNANQSIVMTNNNAQVAANFYGYSRGFENFEPNGKYLYFEQNKGTLTINDLRVSFNYSKTGPTTVVSQQNNDSFTSFVVHDKFDETVARDINFDQMQFSFVNCCCCCCKFFRAIDHPLTEIDWIFDSILTQNQAFQKKINYDTCVALIKRILAYLIMGFSFSLLFYPNSWVVSLSHLVGYFLDSLTGYVYILASFIITIPINLLIIGCVWLRYQPKQGIAFIVFSALIGVAIFYYILRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1U7</accession>
    <name>A0A8S1N1U7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1U7"/>
    <dbReference type="OMA" id="IPCITEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="306" mass="36282" checksum="6EBD40C0A59F3763" modified="2022-10-12" version="1">MIASNSSRLISPRQNFQLKYTKSISKETKYRLRTARYNQLERFTLQKHHPDEIKKCNTSCHCDYCGKMSAFQIKVWRQIPQYESVFSRRKRVFKRFRIAGYAAQFLVFYKAEQIKKQKRQRQMQQFLKNPLISTKRGTMVIQSAKQNRSPKSPTPSENQIRRNATDYFEIDSIKEKMSFNDHCSTLIVCQNQSIKKRQVSLYMEEMLKNITTKQSIGMSLTPLSIIDKKLKKNKSIPCITEQISPKNSLDNLQLEKLLTPLNLLQRSKRNQSNIHIFHNEYSNNNKEVIKMIDNLKIKHRKLKRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N447</accession>
    <name>A0A8S1N447_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0770094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N447"/>
    <dbReference type="OMA" id="FQHEKIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="69"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="705" mass="85203" checksum="8A35DA35F9E8A63D" modified="2022-10-12" version="1">MQQIKGLFTNLLENFEQMISNIDGLQIEIIKQLLEETLEQMYEIHTQLKESNNPTPEKTSVSRATQQKIQDQQTALQHQESEIGHLKQEISQLKNKIMLLNNQLKDKDIQYNELQEQTKKQLDKSSNENKNMKQFYLEIAQLKQTIKQQDDELKLINKQNTDLEDELQQTKENLLIQLENKGQELEINHQQYLSMERKYQAEILFLNQKNQELTQLDQQYKEQFLKQQEEFERFQNLFKEIQNKNQIQFKQQSNDQLIQLQIEFEEYQNKAEEELSAQKQLIKELNTTINQKNQEIILQISQIKDLQQQVDNKDQDQFQAIKEKNTYQLTLKQMEQRFKKQLEDYQEEFENLKQLIEEQTEMNKQNQIRYTEQEELVNNQKQIIEDYIEQIQNHQQEKKKYNREIEKMKIINEGLQDQIDQVNNQNLTAIETSNKELRYQKTLVKQLEQEVIQLTSTIKAQELQVQDLQEQLAQANIIQQSKDLQYRELQQEKQQIDRQFFQSEFNYQEEIKHLELKINQLSENKRRQQQQIETQISQIKSLQNENSDLQEQLNQLNQNIVQMEILEQQQQQSIQFQHEKINRLETQVKIYKQQLQSSKPLSHSFRDSYKTPEKDILENYEQRLQMKQKEIAKLQDMYQQVIEQNELLQSYTEKKNYKKNDQEFDKEFSSQIESILESDVIENPDQERVKLQTIIKNIQKKTQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9R6</accession>
    <name>A0A8S1N9R6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0840085</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9R6"/>
    <dbReference type="OMA" id="GKYRYSN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000029">
        <property type="entry name" value="Kinesin-like protein KIF17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF21">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="5"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="644"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="743"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="382"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="644"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="670"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="89"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8089727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="795" mass="91625" checksum="601AC86FD6031C6E" modified="2022-10-12" version="1">MPAECVKVIVRMRPFNQREKENGSKPCVIVNEDTNTVELKNSQDNDVKSYTYDYVFGAETPQLSIYQKTAFNLVESVADGYNGTIFAYGQTGCGKTFTMIGDPSSETMKGIIPRTFDQIISIINNNSDTNKKFLLRCSYLEIYNEEIHDLLSKDVKQKYDLKEGQQGVFIKDLNIAVVRTTQEMDKFMQLGTQNRSVGATAMNKESSRSHCIFTVYIECSVTDAKGNERITAGKLNLVDLAGSERQSKTQATGDRLKEATKINLSLSALGNVISALVDGKTQHIPYRDSKLTRLLQDSLGGNTKTIMITAISPSDFNFDETLSSLRYASRAKMIKNQPKVNEDPKDALLKEQAEEIKRLKDMLSKQATGQPISFDVFQPIGKLENNSELARLKAENDKLKEKNQIQVVGGPSEEKLKELHEFKEKNNQLLQEKERIEMEVREKEIQAEQERQARKRLEDLLKEKEQMIVQGGRGTEDEKKKYKKLRQAIDLQKKEHEALINQQEQQQQELLQIETKYQSVQEEVEKLRKLVKYLRKKLEEAQNEQKDLKQEVEYEKEDLLETIRSQEKEIKLYSGIVKMMFNQTELESLQAACEWDDDSLEYKIPPFNFKAKKVNFPNLPYKQAIDLIDQEKAERILEINSRQSDINSFNDSEYNQRMTSPKQQRKESQQKQNGISQPQLDRAQLVAEKIKMNYQMLEEKEGKYRYSNEVSNQQFNEKKPNQKIILSPIDNRGNTVPNFPSQYGFNSNTNSSNLSQATPPINKKNINLQPLETKPQIPLSDEQLRRKQHQRVQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAF9</accession>
    <name>A0A8S1NAF9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0760263</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NAF9"/>
    <dbReference type="OMA" id="MYDVEIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641:SF19">
        <property type="entry name" value="NEPHROCYSTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_6G03870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="289"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8086543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="48269" checksum="7BCD7AD9A2B863B5" modified="2022-10-12" version="1">MYDVEITTKSDDEILSLIRTYQQQGYQVWLQKRCSDKFLMQADYNTQGVLDLDNSGIFKSQNVDPKQFNEPSVICSLRKNVEDGHKTSTMEIKDTMLIFPRKTQTDLYSLLSPKLNSEREDLVKGFYEQLKEALHLEQFGQVKGSIALLTNLVNELENQLIDSQILHLIFVESLLTLGILNAQIMKYSDAKMYLEKCLRLFVIKDQILLAQIYRKLGHYSQSVFSYQKSIDYYNQALIIYEIYNLTPEVAYTMMSIAIIYSKVNQFELASKIAYHALEINGSQMNKQTANLYQMLGEVYYNSENYTEALDYFDTSYDMKSTSQDVQSRIRTINFIASSCFHLGEYRKAQELFEVALSQVDDQNSLLEAQLLNNLGITKSALKLDSKEEFEKSLKIFLQFYPQNHPSIKRVLRNDSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBD6</accession>
    <name>A0A8S1NBD6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0770139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBD6"/>
    <dbReference type="OMA" id="FKSYSDN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8086873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="28260" checksum="4075AF4CF58DAF24" modified="2022-10-12" version="1">MSLVSAPKNLKTHLFNLIEQNKTQELIQFIENHRDYIQSLVAPNYWTLGMYAVRYSNHELMKYLYKKDLLLEHPLSQYTLLSTAFLNNDFESFKLLCDEFGKDINKPVHQFQDQTFLQYAIKYNQNEYVEILCSRGAYFIIERTSSSLIDCYANNLKVEQDQIGVKFKSYSDNRDVAQSSIILLKRDKLTPTHYFRFKNILLLQYLLQNKQLQTPEGYGDFQLNFNRILEYTAPSNILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NF77</accession>
    <name>A0A8S1NF77_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0800103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NF77"/>
    <dbReference type="OMA" id="YLKEEWA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="185"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="472" mass="57336" checksum="036D22A53E68F42B" modified="2022-10-12" version="1">MSDYQIKYQEIKGSLTLLLQQNNMLKQQLKEAFEEVQEKQQLKEEWALEVQRVQETIYQLSYDNQNFQQENEQLLRDCQESSYLIEQQIDQITNLKDQNEKLLVQLDQSRQSNLQKEITIKQQKDEFNKKLELQQSNHFQEIKKLQGIIDNQSGLIQSYEKLNEIEKLDTQNSFYQQQMAVEDQMLELKNRNIILTNKITEEKQKYDQLFEQHKDEVLKLQGVIKDQKELISQYERIIERDKKQMQPQSYPQNTSYIESQIIPQQQQQLQLQQYQQQQQQYQQYQQQQYLQQQQQQQQYQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQIYSSKLQYQKYIQPSLSIQTQPLHTQPSSSQPMYTQPMNTNIIYTQPVSHRQPKTFFTNNKINHQLKQQQQKETKNQREYHIPKSSFHLDDDDYQKSGPEFQETFLYKFQNVIGRMEDKLQKMQNELNSSDVSDKPRKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NG31</accession>
    <name>A0A8S1NG31_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0820211</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8089061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NG31"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="57" mass="7034" checksum="A955C8DF30339A11" modified="2022-10-12" version="1">MKKINEVPSINKIYDKETEIKKQREIERKKKIKEELDKQLLKIIQKDIEQKRRKSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NG37</accession>
    <name>A0A8S1NG37_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0880186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NG37"/>
    <dbReference type="OMA" id="SYAANMW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31354">
        <property type="entry name" value="OS01G0793500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31354:SF2">
        <property type="entry name" value="OS01G0793500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035824324" evidence="1">
        <location>
            <begin position="18"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="553" mass="64375" checksum="FE1094DCE06FC5E2" modified="2022-10-12" version="1">MTKYTLIFALLLICSFGKKTIYDRLHGTHIEEEFENEIVTEITMYEEEDPKLKYEKMMNTFMRLNETQVIGCRKTDHHEFFGMFFQNAGRIGNDGQNNIRLIKYEDDCYESMRVSYETNEAENRVCVTFTPGKYKNSQQCSEFYLIGTTLNYNIVNIKDQKEHRVYFNFRNQKQVEAFKYSGAYIFRTCDYLENWFGDLLMTLELFFGGFSSNPYLGPIFGSHPPDWMVRSNIELIERATGYRWQERPNVVVDLKADEVNNGDFLAVTRFDGLDQIIEWGTGGRIGHSAMIFEIDGEKYVIESQDAWYWPTKKIQKTKWEDWKVYAKNAGFNVAVLPLSPEKRAQWDQEAAMEYWKKMEGQPYGYHTFLFGWIDTPKDNYPKPLSAEFATYLFSFIEKIAPGPITSLVGEALNKRLGTEGLSVSEIAIEAAKQGIELSDLYAMVERDEWIYSDGPSQACAAFVTAMYKAAGLFKPFHIEALEFTPKDIYQLKFFDSNYVVPEKCKINDPDLPYCQLMGTHRIELEWYNTVEPYENMNQRCPSIAPEYIRPDGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NIG2</accession>
    <name>A0A8S1NIG2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0890118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NIG2"/>
    <dbReference type="OMA" id="IFFAHLP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876">
        <property type="entry name" value="FI04035P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876:SF8">
        <property type="entry name" value="FI04035P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="1">
        <location>
            <begin position="164"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="336" mass="39396" checksum="9F327F2AB915235C" modified="2022-10-12" version="1">MIQGQSYEVQGLLNAGLDKNEQPIYVLLYNLIIQNKNEEQIVNKFQSYLQQFRPFKGFILIICSQDDTEMKAIDVKNLIKQTIQKVNSQQLKRIDKLIVISNNQNYGKKEKTIPFSLQLLLKDKIHLINNTSFLNLYDIELHQSILRFLCNQIEFQEQQPFYGRELGSYPIGQSGLPVFLEEILQFYIKNKDNLRKQGIFRLSGSHDEEIKLVDQLLQSNYEAIKISDSDVIATVLKNSLINLKTPIFPFEIYQILRDTDPQIPTKEFIDMFSIFFAHLPKVNRLTIFRLIEFIKFVAKFENENMMGLYNLSIIFAPCFFRANNNSITFQIFNSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJV1</accession>
    <name>A0A8S1NJV1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0930096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJV1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8093467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="90" mass="11037" checksum="DECD8B7CF4AE2683" modified="2022-10-12" version="1">MCMFYIYHCIQYKLGYQPNILNYIQYIDFKIRTQHMELHKTNIHFLGCIFLEHMMYSLFHLSNMFYNWQSNHNNVKKSSIGNLNMPNNIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQA7</accession>
    <name>A0A8S1NQA7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0950065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQA7"/>
    <dbReference type="OMA" id="NHEWDIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="748" mass="88125" checksum="8F154B942FE34E92" modified="2022-10-12" version="1">MGTCQQCKQQKTPEIIDIVPSIPRESKSDNIRKIMQLMNEFDSYQIIKTTKYSFVYDDAVRLGRQDNTKTIITQGTVKPINIAKLRTVNEELKQLKNQSNEQDKCVIDKKMVFQCGLELGDTLPQIKFQMLFNEQDQILNLQEGKYTLIKSWSQGEQMVNFEHAIDLIDSLKQFDLQLIGLTRGNIEQKSVYEELVRSNHRWEQFQHIYICTEGYENLDNHILDIYFQENTNEVEEGYLVSALLFYGKKLIWKRYNHEWDIFSDSEEVSQEVTTLIKQGIKEYSGKQETNLVSSITKEQYCQIKKFVLDQQTQQPFTRNINVCVTKKTFFGRNFKKIVYEKPIIEVNCSPKSQQLTESFIKPLGYQSTLWNGQLLKKVLMQKISENNLNIEAALEKQIKIQINYEKNLIQISTWEQSTLIPYYKSTQYISQVKQWIHARDKVLSECDSSDDPVTSRINKMIKDFFTHIPTVEKIQNQQYGKIPYAEKRKPPYEEMSISKSLGNEEITRKKDKILLILLIDYENDDTLDLLKSLIKYKKENQSPLQIAVLGHIRAESWTKLFQAYMEKHKLNNDAEEGIIETWFPVEDKIGSITFSAILSKMYNLVLEKRDCMLVDLNNQIRVLESSRRVIPKIHNYLNDLQNSFQNPEKDIINYKNQYFQFKKCIRLEHNLKDITTKIKQQCKEDDTLILYEQKKSKIWDFENGKISETRKYSQPTKILRLIPKSTEMDTLNSIINKYIDSQDILQMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQU3</accession>
    <name>A0A8S1NQU3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0880086</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQU3"/>
    <dbReference type="OMA" id="DNMAQQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911:SF4">
        <property type="entry name" value="MULTIPLE C2 AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="362"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="409"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="94"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8091475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="507" mass="58908" checksum="24A549C6DF603789" modified="2022-10-12" version="1">MSNKQQQAKDYLTACFQCKGFEPFFIVTKTSLLRILDNMAQQQFDRELANELFERCPTNYKGLVKIEDFIEIIIQADIVLNEKINKANEFIVKQTEDLTKLTNKNRSQVLQIRVIGAKNLHQGLINATNKPYVVVHFNQEKKSSRLAHNDILNPIWDETFIFPIKTGNEQTYISILTLDSTNNKSSLIGEIHFGLRELEDQLKHNQWFNIYDRHGQMSSGSLQLELQLLLDESMFYEQAQQIMKQQILNQQEEKDQYENDLKILYSPFQNGVLQPKRLIIPDAPQLMFVHEFSKPIIYQGDKKVDLTIYKTPQLSDSRVLQILNQPPESVTTVHPEIDYPARHDLKQKANYGYDHVTKDMKLTAIFILLYLLSSIYLCWVKTDFVNITIALCTSLIVGLNYFEKNHLNIFAILIILTLLYDISWLAAYSGVWWNQNKSENPQQESDLITSQRITILVSYFVIFLKIALSCQLWNLKKNIEPLNNHKQFGIGTHLYQANGGHINPFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NR80</accession>
    <name>A0A8S1NR80_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0830078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NR80"/>
    <dbReference type="OMA" id="TNMNIRR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="152" mass="17669" checksum="4F6C35422B5BCCE8" modified="2022-10-12" version="1">MSSDSNKKPIVIIPQTNMNIRRLEIEQVDQSDELEASSEKISEQQSDDPFLNFVTSYSLFTNNSEDTQLDLTQVLQQLNDKISIIKPPSDHLLDFHQQLQIKENQINELADSLKQLPDSKLFSCIQDVEKLYYRLSVDFQEELRIAQKLRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NS84</accession>
    <name>A0A8S1NS84_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0920038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8092936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NS84"/>
    <dbReference type="OMA" id="MIQESNP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="311" mass="36719" checksum="A15852244B57F8A6" modified="2022-10-12" version="1">MNNFSNKKGKQTRAQNKKQAAAPALLKKMKLIQKGKGRYLLEVDETFVNQLQQAKQFLAELNDTIHGQVDLNQGMIQESNPQFEQQEEDGSSSASEDKSAAQLSQQLQEQQSQQPNEVKETQDFYRRIPHFFMGRFKKWAKILKEDDVSSFLHQLKSNKKSKQGRYELGDFHKCFQIDKNDSNEQQKQKKKIKELFLEFLQNEAVLQIIQYNKITNQDQKIKYIDVIPNMVQEMYSDKPFDQFLAQQNIEKQLNKKKQLLTETQEVKQQQQQQQQQQQQQQNQQLPQQKQDQSFEEPLLKLARDMSFQSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NSN7</accession>
    <name>A0A8S1NSN7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0900133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NSN7"/>
    <dbReference type="OMA" id="QEMEHEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="636" mass="76230" checksum="7C54A207862C69DB" modified="2022-10-12" version="1">MKIFSLNFSDQEMEHEYQKEITSIYKNYQKTQKWIVLPLVVFQISIKIMREDWLLLTVLLLCSLLYALNLKCASSSNRYFKDLLLFMSLVLYNFFFPYNSVFQKKELQLQTYSLDGYFQALTILALINTLSYSKKQLILVVIFTFQITLFRNPNIQLWEQVVLHIFELCIYIHYCYCFENFRRKIFIQANTQKILEDNIQTYNDIESFIISYNPPKNKLILMQKNRQQQITEQIQDEFIDKIRNTKVSSYDSKRRFTQFTSQEMLENQANMTLENFLFYEFTNKKKYIMHLIHQSEQNNSYLLKGFLYQESYTIKVVKLYQDKPCALVLLMENKKDNVIDQLNLRNKIAMKALDQIGDVFREYVKPSLISFQWIYNNKQKNKNIVFINKLLRKVNSNLLRCYNDFSNIQDYFSINKEFQRCLIQKFDIKEFIEQIYQNVVRFFGDDKGIFFTIDCKIPDTYVRQDQRQLKQLFLNLFFFILEKQSGSKNNHHNYVHITLVQEKDESNQQMSIKIRIDYEGHHLSKAAINSLPIINPQTIGELKHNSQKAFDLLLPISLLIIRKIGPHDKLTLKQNRMNRNFMEFQIFNALEENYHLLPIISFYPKDNILTTSSLAKHYSEYFDQVPIVQLNSIQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXU9</accession>
    <name>A0A8S1NXU9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1000127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NXU9"/>
    <dbReference type="OMA" id="ITGQGME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="345" mass="40915" checksum="6A9BB351A21AAA93" modified="2022-10-12" version="1">MNQKERVFVDRNNEYAENEYFVGQKESRFHSNDQQINFQVESQLSTLSYIFDWITNDQLQELRSQILHEIKQIDHNVKTKYITTALSYILIKNTSGINETECIQQLLNKFNVKSRKLLKYLRMIKDDGQRDMNKLLQQIQYYQEKVKKYFFGQGQSLLLKLKNPKQTYTQLIEEILETNKQVMIRIFSLQLVKQFCVSKRPSRLVINLGYLAFQIQSIYILPGEYARIFRISINSLRHFSKGLINLLYQFVLRITGQAIEVQLKSCERSPQISQTIMNEGEFKKSPEDTSLSEPNIEIEGYKLLIIRKLINDFTTYQFLFIDTEEEDELINECQEILNFSTNNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZ01</accession>
    <name>A0A8S1NZ01_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1020083</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZ01"/>
    <dbReference type="OMA" id="LMDPPHA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14811">
        <property type="entry name" value="bZIP_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416:SF8">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="153"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="69"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="403"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="403"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8096749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="427" mass="50618" checksum="2416AA59A4623D43" modified="2022-10-12" version="1">MYNQNNEKVVNSLFDLVDNDFMVNYLMDPPHAFPRTNSNQNFIGLQQNFSLLDNQYEQMEMKLEEPIADAKQQKQKKRKTQKSNQSEPIKQKDPFQYKNNLKNTYINKITNLVQQDSQKLLQQQQAKFRTSEEDRHDKNNSVSYQDIEDSTQAKLLRNRECARNSRKRKKIYIELLEHRVKQLNDELEKQKLLNKTSAGYLNKMSQNQQLQGFFLGRQQLYEKLEKSIQNKADDNELNLLLDSMRFRVGGGGKERVSASNYFFNQILEICFPVHVRYMLWAASENKDLFSDQLDQQQQDGQPQWLLDLTNDLQITEAQKKQMKKSQRRIISDKNKLLEILTQFQEIKEQLYNKTSQVENFVDELRNILNPTQVGKFLIGLEKNKFRREMAISKIWNIFDDHSQDEEGDVEVKEEDSIEEPARKKIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P2N2</accession>
    <name>A0A8S1P2N2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1020026</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KFETYYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040132">
        <property type="entry name" value="Tex1/THOC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3 THO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NACHT" evidence="4">
        <location>
            <begin position="763"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1795"/>
            <end position="1827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8096644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1969" mass="233763" checksum="AAC926A1C9148BF1" modified="2022-10-12" version="1">MIINQEYHSLVYNEQRHLRGGGSSISCSDSKKKMNRIYTLIKELNQLEYKIGGPKYQLCQQIKFCLDLIVKIYQDWIQKEINLSDVMNYNSFNKQIENINDILKVQDNWTGKCCLDYYLGLINISLENLQKNISQTKSISIIGLFIQIEKQKNNNENISNLIDAASSLLIGVQCRYFTQFQKFETYYFFILLNKMIKYQVKKGVSIDVNNYFFKIQDQYIKNSTNWTLHNYWIEFLVDLLRFCKNFKKEQKSKKQGAVDVITYITNQDGRNLFDIYLQDFEYEELKQNKWISIDKPEIHRLNRIVNFLTGKVNLIETQFQKLNESYDIEQVKQIFQELYYPLLAFQIYLIEMLQLFQMIQRIQEQNIKIKDNFDKINFIISNIQEQIKSTLDTKITIKFWFKLQMIKNNNYNKDRFLQKKSTLKFQNQIQQLIKLVQVLQNFIIQERMLFTNQIILLNDSLSQFNYGFESQDFDSIQRKSPVIDTYEDLCYQINFHLLWFSHIKKIQKIYYSKQQQETLENIIRNLKVLKQELSKIFFNLTEHIEKCIDKDQQNNENEHEHEQELPYSYQLFYQQIELIIDQKNQDKYKIEECINYHFQTIQNNPGNQKLISSCLQQIQVFLTNKIKQLKKKNQINDIQSIESQMQILSASIRNYSTNYSIQENQDFTDAWKQIESIFQSTKSQFRNESPIQEQNLDANQILSILKRCKIDIALRTKQHDSQNHKIQIKQSQQQLNQVGQGVFEKKLDDVIKQFICKENLNVVLIISGQIGCGKTKSLKKLNKYLQEIQRDDPNSQNWIPIYLNLSDIKEPQNQLLRKAFEKYSTQLNIQDVNKFYSEILQSKYKVVLLLDGYDQLIEQNNIILQNGLDKRIKNLYNEVTLKIIMTIRNDIIPSERFKETKNIQFTEIQLFDNVQQQEYIKQSYKRLITISLSQVQTFRGVQIQEIVEYVNQLFVNIPQLFYVMPQQVEDDIIYKLIGKIKNRIEFFKIIQNEMKKMRYFLENLLIPYLKDILITPQLLKMAIKKWQQIIWQDFEKNDNKIIEVEFKKEFKKLITQVQQGIQEENVYRELKNNKFFKEYHIYDDIPYLENNYTINLNGKHFESSNAALIIQAINVQRFSRYTTLEQFLGFCQEYKVQINLIEDLKIFSQKLSIFLKINNLDALNQQQQNNSQLIINDLEEIFNLQSPYRKAIRESKLITYKNEEITFLNQWILDFFFASKIILLLKFKQEQETIQFLNDVDLSEKIYEGVVQLLIEQLIKIKNIKTVLIQIIKKSINQIMNIRASSNGIFLLSKMGISLEGEDFQQIIIENTSTQMNNLQIDYCNLNSSKLSNAQSDNFLCIEFPQINADQEILQVIIYKENQWLALLKKNGIIKIVNIETQEEQQVDIRNPFMKYKQIAISKDDTKLFALANYQIDLWNLTQYYLASYINVKGESICFYLSPDNTQIICSLYNQYIKVFPLVNQIFQQPKTLQINQDVQQMAINQTNQLLVFRTKQNQIKFFNLQTSQEIIINQQMKANLLTTTNDGSSLIILYGDVITFYQFEITNKDLVKQMEFLIQNTSNPIGLFVSENPQIILIQHQNMIQIFETNKNKLQNVQKMFQEKKLILSGDGKMLINWNNNKVFFWDLIDYIKSCQYSPDGNHLLIQSSEKFTFLNIQAKEFKCIHEKITSLCFNPINNLLVTFSIQGLKFWSTNQMQLLYHFDEFEYFEKQQLIFSQNGEKLVCINKRILICINTKNIFEKNQDQECHKTIKIKKSNNKKIIVKSPNENIYAIQYESLIQIFDYQSSNIITQISLGQSIIFSLSFSQNGQFLAARSSDQIIRIWSTIDFCLVCQFNDQFNNQIENHCLWLSDQQISYIVTNYQNSVILYNISKYLEISSDPSNSSLQNSESIQTSIEKLELKQQNIQQGLLCRNIVISNDNQYIAISYAKQQEDQKQKVPIIIKKTNALKLRWIFIVLLSQFLIFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P408</accession>
    <name>A0A8S1P408_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1050016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P408"/>
    <dbReference type="OMA" id="TYSNCEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051288">
        <property type="entry name" value="Serum_paraoxonase/arylesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11799">
        <property type="entry name" value="PARAOXONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11799:SF12">
        <property type="entry name" value="PARAOXONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="386" mass="44232" checksum="7C3E32EEA1AAA8B4" modified="2022-10-12" version="1">MKIKALIFGASLAFLIKVMIGFFDFFKSVPVVNTYSNCEYLDTNIIGPEDMQKYNQTTIIVGSGDFHKLWSHGKPILEQLGLYAIVNSQDEKPKVVKLGIQNFPTNISLYVHGLDIRKQQDGEYIYALNHAYWNGGERIEVFKILDEHLNLEYQHSIIMDDKYNGILNDLIVIEDNRYLITKYLPYADPRQGRSEMAPSHFIKTLFIWISQQRTSFIIDCKFEKNSTIIQPTCIELLDPSLSGVVLNGITWNQKNLVWAADSIAKQLNEYEITNQGLVFKRYIDIENSIDNLEYDSERNSLILGLIPKMSNYFSLDGFLKGVDRLERQTKFEYYGTVGEYDLTQNKLIYLAQNTELSKGLSGALISGNNLFVGSWCDVTVVICKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P4I4</accession>
    <name>A0A8S1P4I4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1050086</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P4I4"/>
    <dbReference type="OMA" id="KRIRCRQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="433"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="669"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="738"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="802"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="813"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="831"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8097888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="929" mass="109319" checksum="B6A1837C5CD1BE4D" modified="2022-10-12" version="1">MLMHPHINAQQGSSSRSMSISMDDSINMQIQPIVQKMVIVNSHQLQVRKPSNQAQDILRSPPTASLTPQQVSEKLPQNDLKDLLLIDEEPSIKEQSMQINPEPKKKKKQIKNNISNAEMIIDEKPQNSYSNVQWSKLLTSKTSVTNPSFQLNFLQDKAADFFFKQSSLKWLKPKLQNTYYYISSAVLSVYLCFFITIMLIDLEDGIYQQPIYQVILMTFWLIDTFNQILFIQYKNGEEIKQIEDLVIRYCKYQSIFDILTIIPIIMCLIQKKIPAIQLLHLINIWKIRRVFYELQMILGKFALYIYYILELHFVQFIALSIIQFSLDKQINYFDEFIVLFYKSENANVLTYIVRLLILIYYCYFISRLNTQDKWQLNQFPIQNNTKQKINGYINKLKSQQTVDLNFLEYLPQPYIEDFKNQRYMHMLSNIPIFKSSFSESTLKEICNLIQEYTYNPNQTILLQQTPNQQLYLILSGDVRISQRREETQKNCEFKLKVLSEGQIFNNKAFFKNSYSNICATSIGFSQIAQLDIELFQECIKHHYQEKQKYKMMLDQIILQEFYNLCLMRCYACGKFHDIDECDHVHYVPKKSFLVSYIQSNDVQVRHPQKRIRCRQQRTKSYMLKIEENALLYQQLNIESDISDDHKHLDEMISIQQQHYLREVTNPYTDRQNSLQQSQQTPHMASQQSLRESIQSQQQPGHQLMDIPNVSPVQYVQQPPSAISHSSYAQLLKDVSGNRKNFNGSSDKNSSGNVFSLPYNSNGSKTNPNNVIQNKEKEELISIQLKKAKLKDSVYYHQRRIQNQIESEGPHTPSKYQKCQTTAPQLQQNKTYRSDRKSNSCISESEKGEYQVQQQQQQEKPTSKYGITSSKFTGETAKQFMRNPLEMPNIEFYQSFEKSNQYSDYFPQYNVDQAIESYKQYQQSRSSANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5J7</accession>
    <name>A0A8S1P5J7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SF-assemblin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1060147</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF-assemblin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5J7"/>
    <dbReference type="OMA" id="GVESERY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008374">
        <property type="entry name" value="SF_assemblin/giardin_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06705">
        <property type="entry name" value="SF-assemblin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="35"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8098281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="29752" checksum="85BEC9B3F3284C75" modified="2022-10-12" version="1">MYNSRSQVSYPQASPSSGRIQKISEKLSTIQIGVENERFQKLEQAEQRIQQAEDAFNEFQEQIFTKLNGLRDQLGKLQKQVEEDRLAKEQANETKNREVQALEKKFENAIQNETQTRKEGETKVLRLLEDKTALLRTEVQKETAARVDSIEGIHQGLQNDLPKIQEAIRNEANERDESDQNVMKSITDELVKLSNLINVEKRNRDESEQSIFEMLKDIVNRVKVELDQEKKTREQSEEHLLSLLEDTCNKLSIAANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5V3</accession>
    <name>A0A8S1P5V3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1070018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5V3"/>
    <dbReference type="OMA" id="EPIHNKS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="252" mass="27606" checksum="36CE5E653DE18722" modified="2022-10-12" version="1">MNQYLATHDSSLNTSLSKQKYSFPRAIREPIHNKSATNELSYKLPQNLGSRMAGIGYGQKHDFSKDAKPVPAPNLYEIQSFIENNIDKKKGPTFAYSRDQMKAHGIWGSLNQLSPGPGRYASTKSLIDSKFTFGLKPGSLKQLNTPGPGSYEAIQITNQRGNQFISKFRSSGAAIIGKDESRFKTLQTMNSFPEPASYDISNTGITGTGFCPKNGLKSTVQNSFPKAIRKGPFDLGAQSPGPGSYKAYSEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P634</accession>
    <name>A0A8S1P634_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 4 long domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1070067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P634"/>
    <dbReference type="OMA" id="YLNELFC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024790">
        <property type="entry name" value="APC4_long_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12896">
        <property type="entry name" value="ANAPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4 long" evidence="1">
        <location>
            <begin position="196"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="652" mass="76886" checksum="051672CFF7ADB863" modified="2022-10-12" version="1">MEIQSFSPMLDRVMAQEIQSVSWSKTMDVCAILYINNQLEICRVSEQLQRMFIIQEDDTISNIIVESTFLTYNVGNKLFIYQLASHQKIIQSTLIKNQDKITQMLLVQVNVKIIKEQELQPAIIPLSTTNLKNWQNLYFLKQSQINNIIFSLTNRQIAYNFNGIFHLGEIEHKNIIQLQQLENELHCFSDSNIEIYDISLMKHQQILKYIQQLSYLNELFCFLKTSILQINTQLQDIFKCYQLNIIGPLLAFGPQETININILQFYQKGNCPQELVQFFQKELYNTKILQKMDDNITNSFNNIQEIIQDSLMNVLSRILIILNFFIQTKNMPFYQGLNKTPFEELQQTVQKIQKLYSRFQIECHQTKLHLRNFFVWLNLCAIKSGNEQEAECENVNSHLNKIEVDLSKLFDFLKDNHLFQLRNLQCFYQGKISPKTFFTKVVVQKNIEFEQTENITKILQNIFKDSYMEQIQQNKNNIESEESFETVNSLVRELQNNLDKIAINLKPKIINKININLSGQLIRFSEESENSYFVSVLIDNTIIQYTYQQKSFFQFNKIQLNNKYIKELQYFQGKLIILSTNKQENQQNLLMRTQQQKDEGLFNQIQQTAYISTEDLQTQNKFECHSIKDLKVSKKGLISIIIDSKKLIILSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P740</accession>
    <name>A0A8S1P740_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1080089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P740"/>
    <dbReference type="OMA" id="YQTPAKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23107">
        <property type="entry name" value="SYNOVIAL SARCOMA ASSOCIATED SS18 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="435"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="562" mass="65353" checksum="9E4A691DE17B4E8F" modified="2022-10-12" version="1">MKLSLSTEWLFFHLWKKNPDTNSSCPGVFVADTIIYRWAQPYFWYFTAKDGQILRKTKERIFVEQIEEIFQKSGDISALYLQSQQNKIIFEYFEKSEFVQFLHQREKALNALLQKFVEPKSSRNSMIKVSWSPQFCLLSRKTNNNDLKNNKIPIEDRLVTFEGPEHLSTTDSIASPILSSDIEQICLNIVKHIQDVSGGNIQISRMVLFFKIDEKNRLWLLFSSGVKVRQKFINEETVSDFPKQKERIQSPIMILQTQLKIGQTSKFSVDTQGFVKQTGSSCNNCSLQGEMYELTVQQYIASYDQGIFDEETLIIRQKVAKNPVKKSDNEEDYYVEGSLNIPGLLLKIWGKMDIEKYKNLKNNMSFMNLKLQLCLDCYLKFTRIHVESKSEREAKQTVVTIPPLWQPSKTKIQNNQETLTLVSSTKQNKMYHTPSKQVQLPSYQQQKQQSQYQSQQQQQQQQQQQQQQSQMTIQQQPQSSIVIPKLSLPSKDISERIIATTHHTDRQSNFPSKRVAKIYNNLQGVSLPHSQSSLNTTRSTITHSQRIVSIQQMKEILQTEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7I8</accession>
    <name>A0A8S1P7I8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1080133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8099030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7I8"/>
    <dbReference type="OMA" id="IHTYDVV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10289">
        <property type="entry name" value="GST_C_AaRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053836">
        <property type="entry name" value="Arc1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042450">
        <property type="entry name" value="EEF1E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44490">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 1 EPSILON-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44490:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 1 EPSILON-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21972">
        <property type="entry name" value="Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="55"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="202" mass="23085" checksum="2A7EB3482F6AF1DF" modified="2022-10-12" version="1">MELFLSSDLNSQFLTLLVHYTRLVSTGRVKLVVLSNQALKLNEEGIIKIPSIPSLVTEEAQILTNVFSIAQYLTNISFTEKILIGTDNQTLGQVLQVFEAQRKIQGEDLIEAFLKHLESRVFFVTNHVTLADLFLYIHTYDIVSTWNDEQKAGKYVHFFRWFKQVQALPQISEINKTLGRVDVKPASPFVDTQSIGKQKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PC95</accession>
    <name>A0A8S1PC95_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1120059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PC95"/>
    <dbReference type="OMA" id="QMKINTK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="607" mass="71719" checksum="E120F3E9B9F53C56" modified="2022-10-12" version="1">MKVLQFSLSTLQFHNTTLEDKYQEYILEEKLTFFKLLMSITILNTLVSLFRSIIEGWGTFLIISLSVQTALYFITLLFSRKISPYLKYILPLLYVVQYTFFIIGALNNLLQPHYVYGFLGGIFSCCMLNYLSVKIKIVILLSSIWILLGIFRIYSLFNINYILLGIGATSLQMFSAYIFEYNSRLYFSQGMIIKQQINLMYEFTSQKILIVSFNKNKTFFELDFVNQKFDSFYKIENNQDKVKEFLRNHFIINLATHQMFGSLKVQKFANKQITLEDLLFEKYRAQNNFLEQSNSFQIIDQNKNKNEIQVQIINGLKTLFLIIIKENNNDIQIEKYEKKMQMINNLSTNFSILVGSKLQSIYKSIFDLEIYQYETQALNKIQGSIQYILNESKNQLMFFSQNKIHQLIDRCLLSTFILALKPYLIYQCQQSKIQFELLFQMDKDNVQMKINIKQLTQILINIFNKILIYSQSNSTILLRISKEYNLNPLSNSQQKQSSSSKYDYLCSSQDNSQILINFNFLFKTDDEINNFERCFINKDNELEQKDDLITIVNSYIIKQLSPYNKIFIIQEKISRSQNNTLKFYIYSDQTQIDPSYCKSIKQQNIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCS6</accession>
    <name>A0A8S1PCS6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynein axonemal assembly factor 5 TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1140047</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8100976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCS6"/>
    <dbReference type="OMA" id="CFFRINA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000318">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit A gamma isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057978">
        <property type="entry name" value="TPR_DAAF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25757">
        <property type="entry name" value="TPR_DNAAF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="44"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="197"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="236"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="275"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein axonemal assembly factor 5 TPR repeats" evidence="3">
        <location>
            <begin position="290"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="352"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8100976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="573" mass="66163" checksum="9C26224EC399B0CC" modified="2022-10-12" version="1">MSENPFEVFKEDMENEEVYLRVNAIHRVRIICTLIGIDQIKSQLIPYLDSLLNKDEDEVLFAMAEELGNIAEIIPNQSSCLLNLLEKLTTFDETLVREQAVKSISIICQFLSDLEIVNVIVPMWLRLAQNDTYFTCRISAINLMSPIYARAGTQKDIMRLQFFELCKEEALMVRRAVASKIGEIAQYMEKSHVIGELLQAVKELCHDEQDSVRLLCSESLMRITQILNYNEIKAFILPLIILQAEDKSWKVRMALAQIFADLAIAVGKDIADNQLLPICLNLLKDTQSDVRVMGVKSLLKFIKFISPEKLNLIVPNLQILSEDSFSQVKQNVCEVIGLIANLLPKEYCQNKLQKCLFDLMSDENTEVRRNAIKSVGIFVMAIGFEQLNQFIPYLKKSMYDPKWRVRKETIQTIIQLALQVKQLDTFTQQLEEAFLLFLRDRAAEVRSTGLSQLSELISVYKQEWALSNFLQKCVEILEKDNGCFFRINALYAIQQISFAVENYQVEQRLWPIVQKYLKDPIPNIRFVSLKVAKSLLKKIENQDVLIQIKQSINEMLDDPDRDVKFYVQEALQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PDR5</accession>
    <name>A0A8S1PDR5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1140106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8101094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PDR5"/>
    <dbReference type="OMA" id="YQNETYT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="300"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="573" mass="67367" checksum="33E29202BBDC43C1" modified="2022-10-12" version="1">MNSASSNQLLQKLQNLYSTQKKASQHSHSPIKPIATKEINQLLQKSSPVPYLQVKQYDVEQWTDRIVRQIDKHNSFTQNEIPLDIQPQKYSNINTPIEQSLIKSLAHIEKQLKQLRYSKDHDQHTFTTIITKMKLFCLDNSTICFDFYRCTDLILLKFMIYLLILELNQQSNNNKKEQVQDQTQQFQNMVKDIVTIVYEIIEEIKSKQHISTQIDNLNNQLTKIQNSFKPQQNYNSSLKTINKSSLHHRFNSCNLDKNNNTSIHQNKLSTEINLSNNQFKSNPQLNNGSQKIIKYQIDELEQKSKLITSLNEQILKLQNNNKKQIISITDLNAQNESIKSQLKSKIDEIEQLKRSNELLKSSNQLYLEEKEQYFNLYHDFELENSKLKQNLEKYKTAHDQIKTQFDKYQNETYTYYQQQCNQLQQTKDNEIKQLKIELNEKSSIITQLVGDTIFFGKQYKNIVERIVNISQEDLGKEMLELQKELFVSQNTLNAKLNAISSYSDNIMYDSGQEYLQQSQLSNLMVNRTVSSKSGYQQNMKQSKVYELLNGQKNQFELMHMLLMQSQVLEQFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJD3</accession>
    <name>A0A8S1PJD3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1200121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJD3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="19617" checksum="0F13375B691EFE8F" modified="2022-10-12" version="1">MFLICWLPSWVYDSNLFIRLKNLYNNKYKNFQQAQLHFHLICSTLLTLKLFFKDYQFQIFISFHNFSTEILEAISLLDYWAIVNNQKMDKLFPQMDQSTIILDLDPLQKLSQFQFILSLILHLLLLLALFLKNMYRYVWFFTKRYYNITHRTRHKKLLVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLP7</accession>
    <name>A0A8S1PLP7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1230114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLP7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="10193" checksum="57542AE6498756F2" modified="2022-10-12" version="1">MESRSYNFSTAKKRSTQMESKVIFFPREDENAPRTIILKQECPGELAAFKECVVRTKDESKCMQFKDALLECGKPAFRKANTTPDYKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1G7</accession>
    <name>A0A8S1Q1G7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1400099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1G7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="218" mass="26201" checksum="DC141566DE29CF8E" modified="2022-10-12" version="1">MKNYNYLFKYIIVGSTSVGKSCLLLQYVDQKFRDAHQITMGVEFGTNIIKYNSHIIKLHIWDTAGQESYQSMIRAYYRNAIGCILVFDLTDRKSFESLIRWHNEVLACSGNDIQIIIVGNKHDLQNKYTLLYSKIEEKFKKKKHQNLQNNLMQIIQKLLLQMVQMLFRYLKILLHRYFKKFKVVKLIHKMKFMEQKQEILKKEEIHLQFIKLKDLLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2C3</accession>
    <name>A0A8S1Q2C3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Autophagy protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1400070</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in autophagic vesicle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Conjugated with ATG12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2C3"/>
    <dbReference type="OMA" id="MFQSYSD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007239">
        <property type="entry name" value="Atg5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048939">
        <property type="entry name" value="ATG5_UblA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048318">
        <property type="entry name" value="ATG5_UblB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040:SF2">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20638">
        <property type="entry name" value="ATG5_UblA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04106">
        <property type="entry name" value="ATG5_UblB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0072">Autophagy</keyword>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Autophagy protein ATG5 UblA" evidence="3">
        <location>
            <begin position="30"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 UblB" evidence="2">
        <location>
            <begin position="192"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8109292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="268" mass="32445" checksum="1992F65F6E75A09D" modified="2022-10-12" version="1">MDNQYLEEINIEDIRKQFNEQSFLGKFIIQDIDDKTQIIPYVCIVYNNKYPGFYFESIFNHFIRQSNKVKSSLWLSYNDQPIQWHLPFFVQIDKINEEVEIIVHFKKRPENVLPISENIQIDIKNRYFWNLKQACMIRYGHKWEKQIFQTITIEQQYKMFQSYSDHNYKPQFYEFFDKIWDLTNLKKEQQISIPIRIFIDGNMIQRSHVLKDVKETIQNILLPIFEDTLNYNELNINVLGLVVNIDFPVRLLVSTFVNPDGFCYIIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q843</accession>
    <name>A0A8S1Q843_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peroxisomal biogenesis factor 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1490008</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q843"/>
    <dbReference type="OMA" id="CYWVLDN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF50">
        <property type="entry name" value="PEROXIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8111729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="26335" checksum="C067C407ABC7D422" modified="2022-10-12" version="1">MQTLDSTVKWFNKTEGRDKICKVMQYGSRFLMWHLKTNSGNEQLSNQFKNLFQSTRDARKLFRLAKSLNELQTIIDKLGQNCKTSQEQVARALNILTRIWFLLYWVYDNLSVLSSIKFTTSDPKPLQKKAMTFWFIAIITNLVDTIRQLVVNFQYIQQNKNNQSASQQIINKQNQNKTLYLNLIKIFGDLMPAGQGSEFFPKVFKINLNDGVIGLGGLISGAVAAYQAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF38</accession>
    <name>A0A8S1QF38_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040084</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1130055</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1380075</name>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1600071</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF38"/>
    <dbReference type="OMA" id="EDENERW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8042869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8100701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8108821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8114559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="127" mass="15822" checksum="B3EA50C4AC5CB081" modified="2022-10-12" version="1">MVYQWTNFVWETNAWWLRRIRPFVVLGAFAFTAFYGGRYYFFGKWAYYKQRQFTEAELVAQAEVNKRNWGYGVWYKPTLERSRKKLLQDALKDKYRYAMTWEELFYEDPRPVEEILEEENSWEEYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QG73</accession>
    <name>A0A8S1QG73_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T1590037</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000164">
        <property type="protein sequence ID" value="CAD8114286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QG73"/>
    <dbReference type="OMA" id="FMGRTNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000673">
        <property type="entry name" value="CAMK family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="103"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="395"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="467"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8114286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="526" mass="61494" checksum="DB0E148BE11F99EE" modified="2022-10-12" version="1">MGSICQSHSCSYEYTTQLSTSVYFLKPVIDHQIEDEIMEESLSPTDFEKEFTTTKSMVPKSPDVMLKSPQNSHTPNDSVVISKFHITSDDFVKFRYQNFADSYQIERSIGSGSYGEVFIVRNKQTNQLRAMKQIKKQQSSLSNKSLREMEILSKLDHPFIVKAIEVFQDEQSYNMILELLQGTDLQEDIQNNHNFNEEKAAKIGYQLLLALSYIHNQDVVHRDIKPENILYQYNNGNTLIKLIDFGISTEIKKNRKLSSQLGSMYFMAPEIFSKDYGKQIDIWACGVTLYYLVHKRYPFMGRTNQEMKNAIKSGKFQFDKQISPELQCLLGKMLQVNPNKRITAQQALQEDWFIKHKFINQINQQLIFKLINYHSTTLFEELIFSIITYYFQNCDDHGSAIQTFLFLDTDQDGQISRQEFKKQLFQLNIYNIDQQIDDLYITLNKQSDDTLTYKEFLAASVARDKIQTKKCQKFCFQLIDHDQDGKISESDFCHLMGKNHSNLWHLLNPSDSLYITEEEFYSMFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QH83</accession>
    <name>A0A8S1QH83_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIH1D1/2/3 CS-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1620055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8114993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QH83"/>
    <dbReference type="OMA" id="SFTRQND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050734">
        <property type="entry name" value="PIH1/Kintoun_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041442">
        <property type="entry name" value="PIH1D1/2/3_CS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997:SF10">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_56, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18201">
        <property type="entry name" value="PIH1_CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIH1D1/2/3 CS-like" evidence="2">
        <location>
            <begin position="278"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8114993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="366" mass="43107" checksum="086781D23BCC6F5E" modified="2022-10-12" version="1">MDQSQAQHIWKMLDDMATSDPQAYKQFVEKNIKEGMQEMKIEKQQKIEELSIIPQFAYSMKIWGNLLKQSINELESKLLLKQNELKQENFQFDKLEKRIKFYINLLHHERVLGAFDKNDNPTDNPSQYNLIPLSISDVQIGKSTSFNTNVYYYDIVINTDVFKKINRQILQSIIIDTLQKRIELDKNPIKFVYFKAFYKFEIPSLKIINKQYKYCGSKKQLKILLEPQADKEGRRTPQVQNPIENIQQNLSFTRQNDSFNDLIINNQNQNQKKVPNKVLIEEIDTKNDIEKYTIVHEKDKIIITINVNVESFQDIDLNISSQGLKLTTVMDEKIELDFGCQVDNEHPSAKWNKKQKQLKIVVNKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QIP9</accession>
    <name>A0A8S1QIP9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1600075</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QIP9"/>
    <dbReference type="OMA" id="CYDKQVA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000048">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="106"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="535"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="450"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="535"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="559"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="571"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8114567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="581" mass="67801" checksum="98AC089873666C25" modified="2022-10-12" version="1">MLEHKRNHSQKIRTILIRCNDKQVAIDIAMDKSVADLLNKLREHPATPNSQQLVALKTVSNSITMDYMLSVPEERAQLPIHKLKQEALIGVYRQNAHKSILSLLDFTFERCIGKGGTSEVYLVRHHVSGRLFALKMIKKQYITDCRRLEQILREKKILSQVLNHSKFIIPLYATFATKDHLCFLMEYSAGGEMFYHLQNYRFTDDEARAYICEVICALEELHQHRVLYRDLKPENILIDLRGHIQLTDFGLSKLDLSEEQLTNSFCGSPEYMPPEIVNRQGYSYPADFYTLGCLLYELLLGLPPHYSQNTEEIFHKIQNEDISFPEDLSIDVMSLLQSLLNKNITERIHDFNTLKKNSWFNNVDWQAVKQKKSNQMPIFIDLYETHIHQEFLKIDVSDLNQKNEHGELSSSEDLFDFFNYVNETYKDIFQLKQKMRVQSDHLIDKKLLQISQSSTKKKNLQLNLNDIEKQFSKTQKQSLTPQSIMPLSLLRKSFQQMMKEKPRKSQDNHPTLSTVLSDRPIIDRNYIQKMLQTLTSKKKPQSPQHNELSWRPPQVKRGSSSSTHLKSSISQRGQSSTRKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJD8</accession>
    <name>A0A8S1QJD8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1630077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000170">
        <property type="protein sequence ID" value="CAD8115284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJD8"/>
    <dbReference type="OMA" id="ICKMNCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8115284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="23277" checksum="C5391092733F1624" modified="2022-10-12" version="1">MDSHPAHLRNQKKKITLIFQSSQNLNQLSQKLVQTSQSEINVQPGPRLNPNKIQSKCGDTGDLLESGNLLLSNESLGQGLFSQQFTQSGSLLNQYCPHVTLTQEVENCLFEQIKIASTCKFQNICKMNCKMKFNPAYTNVQQILLYQLHENQIVELKNILQKNRGKKIQVCEGQGCEFYFILNDQLPQGNYYCPLCLESFELKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKT3</accession>
    <name>A0A8S1QKT3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Gamma-butyrobetaine hydroxylase-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1700029</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000179">
        <property type="protein sequence ID" value="CAD8116229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKT3"/>
    <dbReference type="OMA" id="WSEEINE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004040">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NUBP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2020.30:FF:000001">
        <property type="entry name" value="fe-S cluster assembly factor HCF101, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010376">
        <property type="entry name" value="GBBH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961:SF2">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06155">
        <property type="entry name" value="GBBH-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-butyrobetaine hydroxylase-like N-terminal" evidence="7">
        <location>
            <begin position="379"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8116229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="473" mass="53649" checksum="A511B142D6F87D98" modified="2022-10-12" version="1">MLRRFSATVPLIKKLYFTFSVNEEYKIQIINRLKQIKQSNSEKDIVSNGHIENLSIDQDGRVIIDLKLDQDYRKIKSLCSDALKQFEWIKNLDIRMAPKKENISTQTNPQKRGNLQNVNKIIAVSSCKGGVGKSTIALNLTFSLQKLGFKVGIFDADVYGPSLPTLIGKEKQQLYAPEDKPKEILPIEFNGVKTMSYGYASGNQKAVIRGPMVSSIVVQLVQQTQWQNLDYLIVDMPPGTGDIQISLCQELNFNGAVIVTTPQRLSFIDVVKGIEMFDVLKVPTLSVVENMAEYVCPNCNHLHRPFGPGYMNMLQKQFGIATALSIPLYEDISKYSDLGSPVVLTLPEDHTINNLYRQLANNVVNELNRTDLSKTPTVRYDTTKRVIIIKDFEGKEKPIKSIELRSKCNCALCVDEFTGKRLNHNQKLDQEVYPQKIEPKGNYAVAIVWSDGHRSSIYPYKRLWSDEINEHKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPD8</accession>
    <name>A0A8S1QPD8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1980005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000207">
        <property type="protein sequence ID" value="CAD8117406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPD8"/>
    <dbReference type="OMA" id="FSSNKWE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="52"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="101"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="147"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="39450" checksum="971483C163321284" modified="2022-10-12" version="1">MQFQLVQEIKQKDWCHALAFNYDESIMAGSIDKSIKLWKFQQGQLQDNNIVLDGHENVIKCIVFSKKNNWFFSGSEDNTIRSWKEKQGWFSSNKWESVKAIKAHQDWVIQLILNEQNNELISCSVDKTIKIWRVSYDSNSIKFLESLEIHQEQILSISLNQSQQQLISWGEDKQVILWEKNQQQKWQFKNKILLSIQEINRGARFLMDNQIICKTSKGQIENYILEKDKYQQKQVLDFNVENPDFNPQECQIIFNIQTQLIVMKHHKYFYFFRNNLDECKFEQVCKPLECQKEVCFFNITNDGKYLVLWIWYPVLRFKIYELIYSNQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQ14</accession>
    <name>A0A8S1QQ14_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1790006</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000188">
        <property type="protein sequence ID" value="CAD8116737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQ14"/>
    <dbReference type="OMA" id="QKNFCEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="252"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8116737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="35982" checksum="83F09163E6FB2733" modified="2022-10-12" version="1">MIQQLNLKLKCQQDDHQDEIDIVCYNQFCTEFRLNCFKCQKKGRIHQDHFDDVEKINSLIEFIENKNKECDNLIDDLNQQLESVNQSFCQLKMGIRQKYQLYQQKLVLLNSQQINDFLNSTIKFNEYKQSITTIIVEQTKKLTNSFNNLYQQLQLSSFNYYKNDDNCIKISEELYQKGYQLYMEDKYEQAIAILDKSIQQNLNNHNSLWCKGAYPKHVRSLCEKGECLRLMKKYNESLKFIDQALSIDQQHIPSLQTKGDCLRDQLKYKEALIYYEKSLKINPNNQYSKNQKNFCENKLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNB7</accession>
    <name>A0A8S1JNB7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8043942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNB7"/>
    <dbReference type="OMA" id="WEWLIFW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="290"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="518" mass="61070" checksum="6D0DEFEB73CC8E5C" modified="2022-10-12" version="1">MSKDYKQLAIQYHLAYMNLKQRIDLSQRAFNTIDFKVQLEVYYQQKLKSVIDKYEDHKYDEVFQYIITNCSNKFQAQEQLITDMKNAKEEFSKNINNYQQQLLNLHDKKEKSINKLKLAQQKLVQSQSFYVSITKVFERNVLTIELVNNNVDQKLGHMKTIQDLMKNKENTFGNYKIAVEKFNQQYNEILPEMELYVNQIKELESNVAECIRQLFQKIALAEVSALRNRQYDLDQVIKYLDKVDFTKINTVFKFTDEAYAPAKVEQFQTFSTKIVNNYLHKFNFAGLDEKNQQDQEQQQLQQEETLLQEQKQKIEEFSTTLLNKQDLSEKLINSIEQSINQNTAYTILQSLSQLIKAPVSIGKRNLSMIQSLFNKLLDFIPKEGPNIHKIQKLSTFIYSQQEEVISLFQILCQNPIFSKKTDYWEWLIFWQVNQTCEGVEDSDRQLIIFQQFQNTVQQMVQAKIPVQIINTFTSDLAPYYGLKAQQIDEIKKLLSKFILAQQSNPSSKLQIQSFDSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNG0</accession>
    <name>A0A8S1JNG0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0050428</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GILT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNG0"/>
    <dbReference type="OMA" id="FRATVCY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004911">
        <property type="entry name" value="Interferon-induced_GILT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234">
        <property type="entry name" value="GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234:SF8">
        <property type="entry name" value="GAMMA-INTERFERON-INDUCIBLE LYSOSOMAL THIOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03227">
        <property type="entry name" value="GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8043774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="187" mass="21064" checksum="3ECE87C182C9D2C2" modified="2022-10-12" version="1">MMFIKDSLYTAITTPNIEQMVHIRLIPYGNTKRKIVDGKWVFTCQHGEAECYGDLIELCAQDSIVKALGAVAAEIPKAGVVHCIENYIQKPYTNNSFVQAALHCQQFYPYEANDVINCASNSYGELLHLIAADETDNLVPKHKGVPWAVANKQFTEETGDEIINNLLRWACQNYDGEKIEACYTQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQ52</accession>
    <name>A0A8S1JQ52_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQ52"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="135" mass="16407" checksum="DBC3D47727264A4E" modified="2022-10-12" version="1">MICIKTFIIFEEHRLIKSNQDFFNILWKLRETEQKHVPTIFKVLIQTQSFSTMQLSEHPSPLSRFPSSQPSSCTYKPSPHNDIFQFHMYSICHYYNQCNMNRKQVYNQCKNLQLMYHHHIIHFQLSIHLHNMEHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQY0</accession>
    <name>A0A8S1JQY0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQY0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="27301" checksum="B2AB0DB4ECD515A8" modified="2022-10-12" version="1">MCLEFGIQKCNQIVKIEQQILDIDILDKLIRKFQEFCYKMTIYKSIIWIIGEKCFTYNWMIKKTKIANQIVVNIISNTNILIFILKIIIYMDIIEKLSLYNNILCNFQFEVKRKNFLRRRQQSYTKQQDYSNRINILYSVVITQKLRFVKYQQEYVKWSYKTYFISISIIIDLIYDCKCRGGQCNQKKFVMLKGKMNQKYGYFRPFSNIVQFQVEIRKQKYQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQY7</accession>
    <name>A0A8S1JQY7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQY7"/>
    <dbReference type="OMA" id="CFQTLQG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="89" mass="10514" checksum="A8529385902EB53C" modified="2022-10-12" version="1">MQFMNETFYKQCFQTLQGLFELKLISKDEKLYLKGQILKLEINADDSTSLEDLSKFLLSCLKQKHHHFIKAPTKSQLDMIDEETDEEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXL5</accession>
    <name>A0A8S1JXL5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cystinosin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0120053</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXL5"/>
    <dbReference type="OMA" id="LAFAYHG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015184">
        <property type="term" value="F:L-cystine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005282">
        <property type="entry name" value="LC_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131:SF5">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8047744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="29226" checksum="09EDB880AE9B4B29" modified="2022-10-12" version="1">MTFLQTLSTLIGWTYFAVWSISFYPQIYENWRLKNVNGLSVDYVGLNITGYICLCIYSTAGYLDKSLDVGTIHNEDLAFAYHGLFCTLIIIGQMIFYPLGDNKLSKFIIVILVILWTLTPIYFLLTQTFDVIDVSVNYNAYRIIGYDKLLISFIKYIPQVYWNYKRKKTIGWNIWVSLLDITGGILSVIQTIMDQFIYDDNNEINPVKFALGLLTIGFDSILIFQHYFLYPPKKDTQVMDYVTMKDNGLDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXQ8</accession>
    <name>A0A8S1JXQ8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0090111</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXQ8"/>
    <dbReference type="OMA" id="INQGQPF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="68"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="381"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="422"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8045086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="60370" checksum="63C13FAE4C413076" modified="2022-10-12" version="1">MGSCAGSGQNKQKQLRDDRIRTEPDKERGSDAFTGGINISNASRKSPDLKGLDLPNSKKKTMKKQESLKVTLSITNGPITKYYIRKNLRYLEGVNFKDNMFVVQHIVTGKIRVAERFSINTLTSAFINKIIYLQLNHPNLLDIYGIFQEDNFYTIISDYCNGGSLLTLLSERNSHFEEDIAAQCCRQIYELMGYLHKYGLYHGQLSLKSFEKYNQGKKQFKLKLVDFSCLFCKSDINENNVQFLSPECCNEKQSYTAARDQWAVGIITMAIAFGKLPFDANSLQECLDNIQLSCKKEGFLQKELEKQIGNPLIKDLIIQFLQVVPSKRIDFFQALESKWMKNYEQITKQQFKQCLDELTKKKEANFLQSCFLIMMIKQFDDQYSQIQEIFNGLDLDKNGRLTKPELVKHYTQYFNGQYKPYEIQEFVNEIFDASDINHSGDLEFSEFLIALSNQKTIINNENLKSIFNQINQGQPFGFNELQPLFRTKEAELRNSLQQIPNQIINFEEFQKLMFELIENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K117</accession>
    <name>A0A8S1K117_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K117"/>
    <dbReference type="OMA" id="RNSYHIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="231" mass="27607" checksum="E5729CC896FB3DC9" modified="2022-10-12" version="1">MQVDSENFIDSSNNPQLIGNRYCMRSRRRKIYDDGDFREVVKNFYALISEINKIANKKIQKTVPLKTKQQSNVQNKKPQQKKPQQQDVKKNDLYNFKKEPIYSQVAFQAQPYFNHQQYQQFSHNYMNPFYNSLPSQLQIISRNQLNNGQLKRNSYHIAISYFIYIQSSSQEQYESRDPTMNAKRKIKETNSDVIKQLDNFITNQKQLPFQQPTHQSYDQEEQKEDIIEQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2J1</accession>
    <name>A0A8S1K2J1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2J1"/>
    <dbReference type="OMA" id="DYESHQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="98"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="212" mass="25223" checksum="7D334014A99565DF" modified="2022-10-12" version="1">MHTSTSITNKHQSFKRVKERLVCEAIEKVERWRLMFTEGLQINNGEIIKVTLNQAAEIVGIPKKTLEDYTQLFKKVKLLTADIQQFSNEKMGFLRSYLRKNQNKLKKKLKEMRLKEIEAQRKTQEIIQEQNQLLKQKLHDDPETTDICGSNQESSNLMIICDDQNYIDADIQFIQEEEDLNFQDFESHQYYFNRVGNFQQEEDIDNYFQSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2M7</accession>
    <name>A0A8S1K2M7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120221</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2M7"/>
    <dbReference type="OMA" id="LPQYQYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="34415" checksum="3E821A82D4B30293" modified="2022-10-12" version="1">MNYNNSEHLNQLLSFQIEDTNEIFYINTYYSPQQLGELFCYQFNLDLTNAKLITQKLLKIIESASHLPQYQYLNEYYERHNDTKCQSNIQFKQSYKLDEFLFDKPQLFDRNKLFQRKIQQNIENIRQQQDQALSIQCTFNPKLNYPNQSQDFNRYQHLYQKGLLKQLERNKNKHIHIDKLDQVDDNCTFQPAINQNYKYQKQKNENLPSQLNIMIKTHLLKQNLYYYQLFQLLDSNNDGLISKNEIDISQLNQQEINHLMPLLMKIEEEDLELDYNQFIKIQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K511</accession>
    <name>A0A8S1K511_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0110408</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K511"/>
    <dbReference type="OMA" id="CKQARPK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="15290" checksum="8F146D54597A493D" modified="2022-10-12" version="1">MGTKRKRVIMQAGSLLNPIFRYVLKMNFVANPTDIHGTKLLRHLQYQTMNLDKKLHQPNFESNYRESIECISSKWEIKFVNGWERTFYMKYADMSTLHQTIKQYNHFIEGQRALQGEDDEPDDNLLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K561</accession>
    <name>A0A8S1K561_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K561"/>
    <dbReference type="OMA" id="NFENDQP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="435"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="631"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="679" mass="81244" checksum="4D1DE8DAB5EA82CB" modified="2022-10-12" version="1">MQQQKEYIPIVLIEDKIQQQKIETEFTEEKLPLIESVFQTNKASNPIITSRTSSIKYSIPIGIKPIVFPNILQRTKEDMIKLRKLQLAQIHIENDQPSNTISLKTENRLKKTERDKSTQKMHTKNSQNKPIITELTTLARDNYSVSPRSTSFKFRSLSPDIESQLWQVNNKIKRINKAYEIKKEIHHLERFKTQWKIDVIQQNLSQKIVQVGLNTKKGLRSIFILIYLPHQNIHLRSSQTEVKSITNEVELSVFPYFTPVYEKIINEKEISDNYKEWFSYFLDILHYRSYIPKAKTFTLDGIEISGLNQIPHYERFLFIQLQGQFDFWNIMKQQLNLSGEFQVDKYPKLAIKLLDLRNKEEVCRFFSKRENLLPFYFQQVQMGSQNSIHSQYYIEIENNIREPDDDMFEQSIIQMNEKEQDTYLKLLQQYIHSNKSKYSNKLKNLEENMRLRLKKIKAMRRQSIQVRLKTKNFIKPEEEEHFERRILYEDEKSIEEIQEDAYDHILPQNTHIFKKKSKQITSVEDPFQSFSSKDNQKFKEILQLINVEKIVLEKNLSRQDVYHYLSLFKALMDSDTPQRIKDVNYPTLFISLDQLRRGVPFIALYKNKVNTKKLAEVYSRRYNYCEFMEFLDIFTTDYQVTEQELQEVEQEIIYQQQKANQTISSIQQKQISLMENEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K580</accession>
    <name>A0A8S1K580_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K580"/>
    <dbReference type="OMA" id="QIFAQKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="22"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="12053" checksum="CCD7D8A8A9FCE7C0" modified="2022-10-12" version="1">MNYLTGQEQRQGGSDQPNQITINVVVNLPQSTLKISVNINHDIPFDTFVNGIKEQIFAQKMKAQYEETIEYLMEDGSIIQSSESRSLQSLGFKNNCTLQVRLMLKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6L1</accession>
    <name>A0A8S1K6L1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0100137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6L1"/>
    <dbReference type="OMA" id="NQDREFA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000051">
        <property type="entry name" value="Protein phosphatase 2C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="71"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8046008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="347" mass="39460" checksum="140A315D3934B915" modified="2022-10-12" version="1">MKLVNIHQSIRSKSENRRQTSREQTNTDHKYKLPQLMDKQISQKTFKKPPLQQKSRRIINSKIPKLEIQLYYGSLSKAGFDGFCEKTNQDREFAEIIDDEQGIFAVMDGHGTDGDQISTFVRDYFQQYAIKDFKTIDFVKLFANAHSNVASHSHFDSLMSGTTATLIVIRDQVIHCAWVGDSRAILCSRQQNKLITTDLSIDHKPHLLKEKKRIENQGGAVNTYKLQNGQSVGPSRVYIKGASFPGLAMSRSIGDQIAEQVGVSHVPDIKQHQITRDDLFIIIGSDGLWEFLDNNQIVEITHQYFLNNDPQGACQKLIQESKTQWKKFSEGVDDITVIVVFLQNSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K848</accession>
    <name>A0A8S1K848_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ku domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0170130</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ku70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K848"/>
    <dbReference type="OMA" id="YKLECQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00788">
        <property type="entry name" value="KU70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.290.10:FF:000001">
        <property type="entry name" value="X-ray repair cross complementing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006165">
        <property type="entry name" value="Ku70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047087">
        <property type="entry name" value="KU70_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005160">
        <property type="entry name" value="Ku_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005161">
        <property type="entry name" value="Ku_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00578">
        <property type="entry name" value="ku70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF2">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03730">
        <property type="entry name" value="Ku_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03731">
        <property type="entry name" value="Ku_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003033">
        <property type="entry name" value="Ku70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ku" evidence="13">
        <location>
            <begin position="305"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="533"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="554"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="581"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8050583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="602" mass="68712" checksum="874183B0590645E4" modified="2022-10-12" version="1">MDFEEEHVGGDDIEEIQDGEDIFGEDADISVHDAGSKKDAVIFLVDCKKALFDMDLDGQGTVFSKILSAFSSFMKAKIISSPDDRIGMIFYNTKSTNNQLKFNNITEIYKLDGPSADIIKNCLKIEQNFEKDYQLGNNAHFHECLWLCNHEFKELDKNKFNMRIFLFTPDDLPYFKDQNARSSALKYAKQLKDADVQIELFPLPSQNEFKIARFYGDIITVDLDEINNAVLDTSTKIMDLHQRIKQKEFKKRALNRLIMDIDDIKIGLKIYCLVNKAKKPTGKPLDRRYNQQLKKKAQFIDEETGQALFPQQISTHLILGNEKIAIPKEYMAKIKGFEKPGMTLIGFKSSSALKDYHNYRASYFLYPDDEHVNGSSQFFDALIQQMILKEKIGIVRLVPKQGSQVRFCALLPQAEQYDENHFQTPPGLHLIFLPYADDIRGLSSVKQEGADITRQTLNAAKILVNALTIQDFDCSNFEDPSIQKFYTYLQGLALQEQNIEEPEDLLQPDFKGMEKYRDIVNLFMSNVSLECSNMPSRSKGQAGGRGRGRGRGRGKQEESDSDDCSKIKGKGRGSTSKQKIEEEDSLEGEEIYQPIKKRGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K878</accession>
    <name>A0A8S1K878_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K878"/>
    <dbReference type="OMA" id="CCRYNQD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00030497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="168" mass="19661" checksum="607D7FBFF2253E59" modified="2022-10-12" version="1">MLLFKPKYSAFFSEIITKKQFKKCCRYNQDGKPLTPIQVKALYETMKPMIEGWKVTDLRLFKYFYTEDYLSGIQFIKDISKIDALSTKNCPSVHLVGGELLTLELFSPSLNGLSQVDFDLAMRINQMNFNEYFLIPLESLENYKKEVQAYKLKKQAEQIQQELAQTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9E0</accession>
    <name>A0A8S1K9E0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0130305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9E0"/>
    <dbReference type="OMA" id="LKYNSQP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="201" mass="23530" checksum="EF0172413DB25C24" modified="2022-10-12" version="1">MMNDSLNKLEMEQTAQFFNPSKKILKQRTIKKLQPIVDEQIVNEFSIPTFSRKLSQQEIFDFDQIVQQQLLKYNSQPNILQINQNFNNLYLLKSSQKSNNIQTPLKSLLGVQQRLIFPKNQSNQIIINEQSKQSTPEKCRRNIRVLLNPFNFERKSSDIQQNKSSSQSLRPSQSQGRTFLQRKAQIGVLNRIFSSRSQDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KF35</accession>
    <name>A0A8S1KF35_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0200248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KF35"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="221" mass="26209" checksum="F55213A3963261C9" modified="2022-10-12" version="1">MVYGIYCLDAVYLFLTNFRIVSLPSQVIMCYQLVMYIYVGTKNQKERHFTLENSLKNIMFREIELAYIYALTYLEQSTSINQIMLILCFLIQLFIFQMTNLINQRGELLQQRAKGLSVWIKLPSKPPGDFVNWNYTQEKYPPNVTVQYNNNYYRLQNLYNTSPPEDIKAIFIQLLFYNIQRTKQRLSIILFIVCVLAVLIDIDALMFNLEIQYTIIPDSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKQ4</accession>
    <name>A0A8S1KKQ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0240121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKQ4"/>
    <dbReference type="OMA" id="EYRINET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="16"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8056060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="704" mass="82589" checksum="51AE050B9CD25306" modified="2022-10-12" version="1">MGVQQSNINENIPFLSNKKMIMDKGNFQIYENDKNQKFDYKEFKSTQNSFQNELEVAREIQNQNFTGMAKIEDVIVQTQEKWFTKYFTLCILIEHPKYSLKEYKQKNDKTLSNQQITELLVSITQAQHILGMKKQYLSLDNIFTNDGNVWKLRPFFDSESSYYNLMRFKQQNIVNFQLDGFPAPEEFFGYICDTDRVQVFNLGMIILELITKQKSNDIYKEYKVDETLLQQRIQSMLKQKNQFCGNFIDIIIEMLDTDSVRRPNFEQLLKKLKSPSSQIVYLQTKINFVKEIPKLNLLDSINLFEESSLNKYDEAKLSKALQQAKIEISDYIIKTKQELQNIKNAQTKLDKNGQKYHGQIVNNVYEGKGYLFSKSGDLIYEGEFSKGYFHGWGVEYHQNSIQLEESYNFEVCQDIMEYFKKYEGCYQFGKKSGVGRLILTNGEIFCGEFRNDQINGFGEFQNKSNNKIIGIWNNGLIKSNSGLQNNLISFRSDEFQNQTEQLQIQIQSNQQQNQGDQKVNKPEKQFNGISNLESCRSFLEQSTINHLKYFNQNQSQNRKDHKIFYDDANTILKYEGQLFTGQMHGRGTLYFKNGTIQYEGDFVNGKYEGYGILKNENPQKEMNINYNDLRDVNIKGWWEQYQGTFSKGEKQGQGYWHLTDNSVLLGMFEKDEVHGDGYLKRPNQEDFYAQWENGILHKVLQGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KPV6</accession>
    <name>A0A8S1KPV6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0230045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KPV6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="60"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="176" mass="20902" checksum="EFA025A300536837" modified="2022-10-12" version="1">MKAYLRYEGISKQQYPQSPLQVEPKPLFTMMPRRKTAQVPFRRSLHQNKSPTNFDRQFVVEELRLANASLQEVNKELQQLNESLHEQVQKLQESNKALQIQINQMTIQIDDLKINNRHLRSQDSSIHFQSKAFDQRFKVIEIQDYKIKIIISCIYSCNQKSQMTNQNKKELQKPNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSZ7</accession>
    <name>A0A8S1KSZ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8058027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSZ7"/>
    <dbReference type="OMA" id="TINMVNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="404" mass="47721" checksum="4398C1792EE97B78" modified="2022-10-12" version="1">MQTTLEIEKKLCQVHKLEIVAVDLKSQTRIDDKYLCTRCLAERVDKENMALLNEATEMIQSMKTQSLKIAKEENVLRLTNLKQLQSSIKSFKNQIKTELGKLLQLVDQQMEQIQTEIESKDSKLEIKNYDEEIQILSKNYIGNFSYNIPKQQTYKEKDVALVQLIYESLQSQSKSQNFIQIMESIDQIRSSIPITQQAKINETPAKEFDQHKTPCLNQICNKHNKEIIMLNVNYNEPKFGRLACVECIQENPIQYISLKEANNMWNTLIGESEDLISKHNFKREKTFKMVIQEIKQLKNHYNNQLSEMLSSIDEQLIQNNQEFQDFLKLENKQIFELDEKQVEKMIDLLSQQDKNKHILQQQEKQDRLDQLFYQNVKSKLETLIKHDLLCKITRKKRNRKQSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTH8</accession>
    <name>A0A8S1KTH8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Anoctamin transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0210374</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="RDTIRIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF73">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="865"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="938"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1015"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1186"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1226"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="577"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="7">
        <location>
            <begin position="821"/>
            <end position="1263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8054254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1282" mass="151436" checksum="7D67B6C3E12F4DA2" modified="2022-10-12" version="1">MFKITQTQILEWVKDGNTETLRIFFESSLQQNQDGLNSFFKMIDKEKRNVVHWAAYLGQFGLLEKWLTSYSQFIDLNQVDLHHYTAVELASIKGFMKEYNSQVQFALIKLLLDHHAKMPTDNKYKRANPLHWAYYYGNSDLIDFLLSNYTDLQYQLDQFDMYPIDYLFLENRPEEYQKKYKAIFAKTVQEYAFKKDGHQFKFLRNLNTQRTIAIISISQKSNNLDNPIMINDQNQPKLKEPANELDSIDQEKINQHLQCGQTTMTLMSKIQQTGINNQKRDSISSRISQQFKLIQERMKKLPNKNVQNELPFEDSQQDFHEPDAEKIPSLKIIQPTNTIFSEPHFNIKEKIENQIVFQNDLESDRNQQKVFLQQDDEFGRLFKCINIPSRSEQSKYMASQYQQSQAPSQFGRQLSQEIQLSQEDFRPSIKDFSRLKSMRPSLKKPGQKLITRKSTTFNETYTIYHYAKSQTKNQLFECRLQFWSARIESTEFFVYFLKQKCNPFLIQYQGFNCLHIAANKGKYQILKHILESEYEYQELKDLLKLHRSITQGQFSKTYVKKDIFNKVEAFNIMTDQNPSNALHLAIEINNFECMKILVEHGVSMDVLNHRCLMPIELTFDEKMVKYYELHYLKGKEQSFNFMGYMYVIQTSGSQDLCQDIVLLQLQNIKQSFQQRNMEFEFLIINTPNYNYIKNGREHCVKHHYYVIKLSADTIYKLADIYQIECYHFTKKHLTKFKYRDYGHFEFPKPLQIQQLIINVLNEEFDLDKFVLEGLVISHFPLEDSSKSQKVNVLWKELQYNCIRDTIRIQTHQIALRPLNSIASYYGPVIAWYIAFNVQIVGWLILPALVGSAIQLYQLIADKIHASILPSYALFMSLWATLFMEKWKNRESELKYIWDMHKFKQQEPQRVMYTGLYTVEPCKNKVEVYDSFTTFKRRLIAEFSVIMLGFSIILVNFLAFNQWQGQQDPQSVYMPIIINSLNGVSMTVLCDLYKRLCKFVVNWENHRFNSEMQHSYVLKVFLFEFLISYISVVYAVLFKTDQTQLTLSVASIIITRGLISNLQSNCLPYLLYKYLKWGLKDKFQTFKAFKEQFKICDMQYVNEKLKQAQQIEFMKLMEDSNNKQPQKELYEEYTNIAIQFGYTTMFSPAFATAPLFFLLNQFINLQFSISNYQSVLRRERAQAADSIGIWLSIFELMNYCSTFMNCLVIGIVNKAEFEGLIGDSNPLIQVLVLAAIEHILLLIKYLLGIMIPDCPYWVSKELRKYAYFEGQSAKIHDDSEIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWF4</accession>
    <name>A0A8S1KWF4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0280250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWF4"/>
    <dbReference type="OMA" id="INVKHPS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="280"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="367" mass="43433" checksum="1339D49787E3F081" modified="2022-10-12" version="1">MNNYKLQKVLFKKVNKGVIVEEHLLVRSLIDKQCYILKRTDISKINAERLKEIENHLKQLINIKHPSLTKIKEYFIESQYLIQVLEYCGEQILNERLNKQVSVNLVCQYLLQLLFALNYLEIQQINVSLDLDTIFLNSFGSLRIQFDLLSHKNWKDEVKYLQQVLKKTNLDWESQKSHELTQILKSLMQPNPPSVQQMINHPYLLKAMFDFCKQQDLEAQAQLNQEKLIKEQTNNKRVGRASTSANQHRQKITQQSPTQRKNSKDRPQTNQQQKKKPEQQIKQQPIKNQIPPRPIRQRNNSQDIVINESILNQDLLMTKGKQELQELLKLYKDQLKESSKQYNPWLNMSEIKEITEYDTTTNLNQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXN8</accession>
    <name>A0A8S1KXN8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Insulin-degrading enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0300030</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXN8"/>
    <dbReference type="OMA" id="VEPWYCT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000005">
        <property type="entry name" value="nardilysin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000012">
        <property type="entry name" value="Protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000165">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="9">
        <location>
            <begin position="25"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="10">
        <location>
            <begin position="177"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="11">
        <location>
            <begin position="359"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="12">
        <location>
            <begin position="743"/>
            <end position="840"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8060229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="926" mass="108202" checksum="E68EE62570EDC773" modified="2022-10-12" version="1">MIKLSKVDKRIYKHLNLSNKLQCLLIEDKEAEKSAAALNVQVGAFQDPRNAEGLAHFCEHMLFMGTTKYPDESEYQHFISKHSGMTNAYTSSTNTNYFFTVANDQLEGALDRFSQFFKYPLFKESCIQREMKAVHSEFNMNLQNDFWRKFQISKLLAPQNSSYSQFMIGNLDTLGQVNRQQLIDFHSRYYSSNLMKLVIYGKQSVEQLENWAIDMFSDIPNKNYNRPNVNIEGSQIIQNKLIKIVPINDEDHLDLIWVIDYLHPHFQNCPGKYIAHLIGHEGEGSLLSYLIKENLAYELQCGTQDEAYKFSELYVSIKLTKKGLAMYQHIIELVFNYINILQANAQIFNEVKQIKTLQFDYLEKQNPFDFVGALASRLHQYPVSDILKAPYLMENFDSNLINKTINQLNRNNLNVFLQSQQFQGKLGNFEKYFGSEYEISDLQFENQQIRNPNFHLPNLNKYLPNSTDLFANPNSQQYPTIIYESPYSTVYFKQDNKFNVPKTFIKIRQYLDQMGKSIQNEVLGALWQSLLTIHLRELFYEAEVASLSPSVSLVTNGIEYSLAGFSDSINKFLPDMLKKVLDFKVENYKDNYDTQLAKLICDLENFSRSPPYSQARTLSMLLLRDCGSFDPEDLLKTIRLIQFDDLIYFQNHFMDKCRFEWLIMGNIQESNAVSIVKQSEELFKKSLILQKEEVLQVRSINIPENIIYNYTRYLNSETETNSSVILYFQLESGTVRNQLIVDLLSNIIKTPFFSQLRTKEQLGYVVFSASSDVRGITGFQFLIQSSVKCPKYLQARIREFIKTFGIDDLTREQFEEYKQSIRVSLLEKDFSLGREVGRFWGEIQRHQNLFDRREQALSLLDNIDIKEVKNYYKQYLIEHPHQIEIHVVSPSHKQEQDSLPNTGLMSTSVEWIKRRVPLYPDMYSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5R7</accession>
    <name>A0A8S1L5R7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0260323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5R7"/>
    <dbReference type="OMA" id="EYANQQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="264" mass="31564" checksum="7B1BD7A7FFD19324" modified="2022-10-12" version="1">MIIANETQLLSSQSQQNIEFQVQNTVNNQIHIYRQLRANDSQIQNNSFHYDDLDPQIRRKCLNLRNKIHFENQKDELEFEDIIKQWNQSLQQNTRLNQMVTEQLNQQYQNNQYQTDFPVSKIHILDYEENQPITSFSLFTSKDLIIENLILQDIPLEKSTQTDYPNQKDDKFTQTDLIDIDKDDTENVVEFFNSSFYKDLKNKQFLIDAQLISLIQTMFLMHNERKIQINEPIKQQLDLLMKKIQKKDQINYNTIVIIKDSVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6J6</accession>
    <name>A0A8S1L6J6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0330093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6J6"/>
    <dbReference type="OMA" id="LICKESY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8062351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="267" mass="31898" checksum="8C257AA8B54D1F7C" modified="2022-10-12" version="1">MLTGKEEQPLLLLRSNRGNQESKELPKYLRSTFTDTYQLQEPVKEKRGKKILPQYAFGNSLESPSFFRNANTLICKESYDNPFLKGQQKPKSKWESLRSNTLNNYDFYNISEQKFSNMPSEGFHNIRLVNTVTNQINFVKTPKVKNQDYTFQQAYRTLQKTSHFRRKHELFTDFIEKNDNQNVYGHLNDRQRPKGHLRDVKNFSLTPQEVQIQHKYNFNSMITSPKYKTSRKQEFPRVNKADEKIEKYFKRDQKQIVIYVLQVFLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9Z5</accession>
    <name>A0A8S1L9Z5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0350221</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9Z5"/>
    <dbReference type="OMA" id="MPMKLPR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LITAF" evidence="7">
        <location>
            <begin position="73"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8063949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="160" mass="18312" checksum="78E31CDB59D52AC9" modified="2022-10-12" version="1">MQIHNNQQPGVNQGYNQYQQPSQQQYQGINNYNYPSVQTQPQYIPQQQQPLQGQPINNQDQQYVYGAPIGMAQGMPMKLPRTADGVRFPVEIECQFCRRNTKTRIEYKSGQSVCCCSCLLLFTIPILACIPCIMNSQKDVVHYCTQCNHQVGFAQKKLCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LC94</accession>
    <name>A0A8S1LC94_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0340146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LC94"/>
    <dbReference type="OMA" id="NDHNPLA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="421" mass="51090" checksum="1FE2ACB0F8DFFB25" modified="2022-10-12" version="1">MSDEEEEVEEEEEEQEEEEEQEEQKPQKQENDELNEFRKYNPHSSYANMHKYPKNHNDHNPLAYYYQRQLPIPYQPSQYWMERVQYHQKEIDRAKQVLLNVQKPQPNMYNYERSLNYQPNNSPLPQRVQIESQKSLIPPYQQPSYNFEPRDFNRSYQAQVQPPYPYNFNPQSSKYNSYPPPPQQSYQPPPQQNYYPHPQYDLQNSQFSGNPNINPQYQPYSPYQNYYPPPPPFAQVPPPYNFQQDLMNEYPKVNLQSSKQVQTEKIQDLPQLPTGISQFQQEKPQPFSQQNIAPTQPLSETELEYIEFLRFKEFNQKQQQQIDDQDFQEFQEFRKLKQQQLFKQTVDREEEKRSLGDLYKRKNRQVYSEQEYKSQLDKKRDYQQFQQPYTKPLQQETNLEIDRAINALMRSQTGTNKDFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LE91</accession>
    <name>A0A8S1LE91_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0390063</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LE91"/>
    <dbReference type="OMA" id="IRISHPF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05580">
        <property type="entry name" value="STKc_PKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="53"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="312"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8066388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="365" mass="42856" checksum="870587C90D0CB8A5" modified="2022-10-12" version="1">MNQLQIEELQRKLQKLQLERMNEDSSDEEPTFQSVQGKPNDKQLFQNLSIDSFEVYTTLGTGTFGRVKQVRIKRDPSRQVYALKIMKKHDIIKLKQVDHIKSEKNILNEIQHPFLVQLKGSFQDAKCIYMLFEFVSGGELFSRLRKDGRFSQDITLFYVSEILLAIQHLHRKDIVYRDLKPENLLIDREGHIKIADFGFAKKIKNNHTQTLCGTPEYLAPELIQGAKTGYGKSIDWWALGVLIFEMLAGHPPFYDIEPTNIYKKILNGVIEFPKFLHVRAKDVIRKLLNSDVNKRLGVEDEGAALMNHKFFRGVPWQKVYEKKIQPPWIPFLRNETDSQWFDKYPEERDDIQPIDDEKQHMFDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LF84</accession>
    <name>A0A8S1LF84_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0370108</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LF84"/>
    <dbReference type="OMA" id="LWTACKK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000140">
        <property type="entry name" value="Protein phosphatase 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8065121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="282" mass="31773" checksum="3315F5E36D3D335C" modified="2022-10-12" version="1">MGSFLSQPITQKSHEYQFSGNLSCYTTAMQGWRLQMEDDHLMKPNFLENISLFAVFDGHGGSGISKFLAENFINVLISQPTFEKMDFMQSLHDTFLQLDDMIKNNEIKNTFIGSTAVVALIADKMLYVANLGDSRCLLMRDDETIELTKDHLPSNELARIRYAGGFVDEQGRLNGTLSVSRAFGDFEFKQEPLPANQQMVIAEPEIRKIKLNKDDRFLFLGCDGVFETQNSFKVMEFISARVAEKQEPSIILEQLLDTSLAADTSTGYGCDNMTAMLILLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFF3</accession>
    <name>A0A8S1LFF3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0370148</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ANIRYAM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23810">
        <property type="entry name" value="UBCc_BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000146">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF4">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="4">
        <location>
            <begin position="601"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="333"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="347"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8065201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1851" mass="215237" checksum="A6E22ABA11B931B7" modified="2022-10-12" version="1">MFYEDYGQDVYIFEEQTIEEIQNNIEFTTLEEYKKLTTFEKIYQQKSLYQHIKSLKNDYLKNICIKELSNSLVENCDEQLVIQVYDDILDTPLNDLPLQLLSKSSYVVSSDIPEVFLQEFPHSVNNKYAWIDKGDQIQYGLEKPCILIYPNVILGMQKTFNKTDLKCQADLVYYNNQLYQNQTVYFEQKGIRIMGQQFSKLEKFYVYQQSNNIDKTKTKLCKKLSKKGLIHSLLIQLAQEKFENKSSVNMLLYECLLFENQDWCQRIISQDWLNNVITLCLEDPKDISIFHQVVYDNLLQIDLNNYTEQQVDQLLLRLHQYYEANSLDLKQKKVEKQPPKKVEEPPKTTQQTTNSNNKQKGSKPKGIGYGDINYSSTYQSQQKKKEVQVLDSDLIYQQAEGYRWKILNEIFKKLKHLNLDYILMIEDSCLIPVIKSIISSLSKSQTISDKFEVIQIVFVLLNKFLTEEAMHLFTCKDYGDISLFEVIQKVNNESVFIQNKDNQELNQKIKQNFTNQDTQLSEVLKELQDFFNKLIIILEQNKYIDVKFEETETQQIQDPAYLYPMIMKPLVLRTSEVIKQAGWKKQLDEAYSHLSSSPPNKKMNRIIKELSDIGENLPLQLTNSIFLRYDKDRMDAMQAMIFGSSGTPYAHGAFLYNFLFCDDYPSRPPKCLLETTGHGKVRFNPNLYNCGKVCLSLLGTWGDNWKPNESTLWQILISIQAMVMSEYVYFNEPGWESSMGTVDGEKNNRGYCNIVKLANIRYAMIEQLQKPPVGFEEVIQKSFYLRKDVIIKEVQQWIDEADLPVNYHCTQNHNISAPFQQQPQKFKSILTAEFEALKKELEKLKVCITHQSDKKILSIMQRQSYTQIQQEKKKQHDKKKEEISEFLKSQLPEIDVSESNVSNRQFDIQNAEVQNLMSRYIGVMGLDSVSKQSQASVIIYGLGALGIETAKNLVLSGLKRLTIIEDKKLNNQGQFFVQNEDSTRLEQSLLHLQGLNPYVQIDYSNDIISSIKSQNYQVVCLCEVDSLKDINIISQICREYKIGMIVSQLVSVYGRIMIDLGDQFIVNDADGEQVQEFIIENIDQEQGIIEIKGKHNLSPNDVIELKEIIQENGKSLNNQQLNVVKVINKSTIFVGDLSQFGKYLRNGRGQTVKQKIILQNKQMSTIIIDPIFDPNFILDEQKFTIINEQMNRYSNQSGDINELFQKTGNQIFPPQAAYLGGIVCQEIIKAITHKYMPIRQCYFHTCEELLDGNHILGKDLQLAIEKCKVLLIGAGAIGCELLKNYAMIGLGINGNIIVTDPDVIEKSNLSRQFLFREKHLRQPKSYTAARACMKMNPQMKIVARLDKISPQTERLYTNVFQYVDIITNALDNVQARLYVDSQCIEHMKPLLESGTLGPKGHVQVILPNLTESYGSKQDPEENNEIPYCTLKMFPEDSNHCLEWARDKFEKLFTTKIQQIRQTFLFKDFTIEGLETTLKFCQNMPKNFNDCIQYALNKFYKYFVYGIMDLLKAYPLDHVVNGKLFWSSPKRPPQIFEFKGEEMQMKFIQSVSQLYATALGIEIPQQIDYEQTLKNIKPKEYKENKEKLQQIQDQVQKDAQAKVQEEGNQDNQQQSQQELINQIIEYFKDYYEMTDSTPKLLKSLDFLPQPIQFEKDEDDNHHVEFIQAALNCRAQNYGLEPLDWLTTKLKAGRIVPAMATTTACIAGLQTIELVKVIKKLMIDENLKLETFKNMFLNLAIPYALQSEPGECQYEQINGKNFSFWSKWDVHFSSQVNNLEKFITYTEQEFGEKVTCIKQKAKLLYSPIMFNSKEEEQNLLQTPIYLLFEIQDFEEYVELDITFQKRIKCRFYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHR9</accession>
    <name>A0A8S1LHR9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Aurora kinase</fullName>
            <ecNumber evidence="15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T0380078</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="15"/>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHR9"/>
    <dbReference type="OMA" id="DWAISHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001241">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="17">
        <location>
            <begin position="5"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="56"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="130"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="179"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="11">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8065785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="404" mass="47336" checksum="649F815B9BBACCB8" modified="2022-10-12" version="1">MMSKCKSCQDEGFLTCPLCQKITYCSKKCRDYDWAISHKFDCKSQLPKRSVSEFEIVNQGSLGRGSFGCVKLARDKQTGLLYAMKIIEKQGVSIENLKREIRIQKKLQHPHVIQLFEFFEDDQNVYLILEYAENGSLFGYLRKRKILNENESFVYFFQTCLGIDYLHKKQIIHRDLKPENLLLDKDGNIKICDFGWSAEIMITQTRNTFCGTIDYMTPEMLEDKPHDQTLDMWCLGVLLYELIHGTAPFKGRNDFEKCQNILKQENFEILASDQAKDLILGLMKRDSKQRLTMDQVFSHPWMLKMAKEYNVEIKEYIFEEKKNLDELSSSLRLDTKFSNRNTTTTRLSGSNFKKECNSIAFSMYSEDMQPQIQNRITRRTDHSGKKEGGFFETLFQNFGCTRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LN54</accession>
    <name>A0A8S1LN54_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0380205</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LN54"/>
    <dbReference type="OMA" id="HATEAIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034663">
        <property type="term" value="C:endoplasmic reticulum chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000029">
        <property type="entry name" value="Heat shock protein 110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF3">
        <property type="entry name" value="HYPOXIA UP-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8065976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="550" mass="64580" checksum="8FE34A9DB638279C" modified="2022-10-12" version="1">MQEEYQGYGVDLGGRYTLLAKCQLFSADVVLNEGQRETENVIVFQTHERFFGNTAAHSYRSNILNSVRGFQKPHTFQYKGKSIKMEPEQQIGAFLRKLLRNCNRTIVISYPDFYTQFDLIRLWRAIQISKIKVLAAIPENMAISTYVNYCQNNVLQKEMMIIDFGHSKFTAFLLSQEQVICEVFDRNLGTFQMDNYLAEWIINKAKITIDKQSKYYLRLLDTCENIRKRLSANKDTHIDIEQLIPDVDFTYNLTREQYETIIQPVLESIQNLLFNLYHQTQNYKFDLIVTLGGGSRIPIIQQCIKTIFEGKQIQSTIHATEAICQGCAVSNTLITQRKEQLKKQQSTHHYLNLYEHYPIFIGIKTQDQNNNQTHQINLREYFVLNGECQKYSHCLTKQLDQFKSYEISLFYDPPPIGFQSLIKTYYITNTKIIMFYYDDYGVVQMTADNQIALPIQDQRIGLIEMIEIEDQLELDDSNVQLTHDLRYQCERRINEVKQQYKNINLSVPQLMKVGELIEESEQRVLDQSLTYQDVQNLMNEFEIQYKNIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSK9</accession>
    <name>A0A8S1LSK9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0460038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSK9"/>
    <dbReference type="OMA" id="SKTIEMC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="274" mass="31586" checksum="3B46F5404977CBD4" modified="2022-10-12" version="1">MSQQLSMQKSATQSTLRTLTDYGGQQRLVNYANLQRNLSPQAELCLAKDYNSKINKYVKQEKTTCTLPSECDSKTIEMCKENLNKIATLQKNNPTLKSSDPITQESRPLNENKSHIIKTPLSDVDNLWNFHNNPIVSRQNPNSKVNYQTQQPDWKSSQKSIQPAPAKTKDLFEKRELKVADSKPADRNPILDNNQGKQYGKHESYGKSDHLECKKLLSNGYGQELKYKEDKHLAQKPNSFQQSERTFNYSTVNQGDYRKKPSEFENKNTFFTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LU76</accession>
    <name>A0A8S1LU76_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0380276</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8066114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LU76"/>
    <dbReference type="OMA" id="TNIVEHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46726">
        <property type="entry name" value="TWO PORE CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46726:SF1">
        <property type="entry name" value="TWO-PORE CALCIUM CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="282"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="566"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="669"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="361"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8066114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1201" mass="141817" checksum="F8DDCA64F8AFC0DC" modified="2022-10-12" version="1">MNKPLKKRRQSTIYRQSMALQEFSQKLLEKNDLLASEIQKIVRKLSVKQRMKKAIRHIYDAYYLRTIRNDIDLTNKKYLKFFWYLENNQFWIGFLFLLSIIYQIITFFELPFSDAEIEDDHNLLILEGIILTFLGIDSIITIILMVTKKENRFAFNPKRQFKLIVYYVCLIDFIIYNKDPNTMRFSRIFRTFIMPMYSKDLRRNLKGIMKASRDLFLLILLYLIIISIFSFVGINLIGRLENVDLRTQDYGDFFKFFSMLFMVATLDFYPDILIPPMLQGTYYSLFFIIYLLLFIFLFAPIPLAVVYEGFRRHRMEIAINDIIKQKSAMMASFISLDLTDQGYLTHAQFQEFVEQFYENSISKEDMTTLFSLIDQDFNDKVQFDEFYKFLHLIQDGSIFKLPERKPLECWESFRLYLNSRGLLKFVEGNVFSIGMLIITISNCVLIVTAFFIDDMKILDIFTLLDTVYLIFYGVECIVKIIALGIKPYFQEGWNIFDISLVILQIIFDYILFNIVSGNIVQSIKANRLLRLAKIQKVFRLFRAFRSIKIINFMLSGLEFLDIVRHLLYKIIICVPLIIRLMLPVQMIFFIYSCVGMYLFGKIQRNPENPYANSQCDSNLFEYQWGNCKYADFTSFSGSYLMMLQMFIAAEWNQVVFELTYDTDDMLSAMLFVGSFEFLSIFLLALIGGLVWEVFSVVSMQLRQAEEQIPQENEISQDQDIQAFSGTQIDSKYTPETAANQLRKKTLILNDDNPDVIKFQRKLRSDKKSKSLTKVYNPQRSISEHLIKDEKPDLAFSQPGSRNHLLPNYNSNRKPVGLYNTQFDNKIGTNIVEHYEQHFIDYQQWVIKQQGDGLDMNQVANDYMIHLRNEIKKDEIFQKKNVNISNHHLLIQSAVLRDASEVYIKQQEDRFFKMSFGKKFERIRELKFQNKFKVESKIIFSLMGILKFPKPNVKYYFQILYLIENYFTYQLLSDSSFFKMIHQINNKWYVIGIEDGQISFQKLDAGPWDYSEELFLQTNMRICQNLRSLYDTGNQECLQNVNKFSESVKLLAKHFDINLGSIDTNSSCLMYQIKNDKFIPSNIDTHSWKMKKSNVTIDDQSPNHNQIVFLESQNLINGQGFNKKDQSISLDEYKYVSKTIMFVLTQTQAKEKRIIYQNLIMLAQFLQDLVGVIHNYESNFFKQIDNLFELRNQQKVKSVPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LWR8</accession>
    <name>A0A8S1LWR8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0500262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LWR8"/>
    <dbReference type="OMA" id="LINEYDF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="123" mass="14408" checksum="4FEA49B30BB6AD4A" modified="2022-10-12" version="1">MNFDDASLSSLHTYRVKTRQQLKGIQHSIKKSKRKYCHISPDRKKQLISLVLDKKLRIKDAAIICNLNYSTAKTILYTLRHRPPKQAGFKQFSSKCKPRLIMKTLIKGTLINEYDFYSYLNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZ44</accession>
    <name>A0A8S1LZ44_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0500303</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1584" mass="187212" checksum="CEA08532926FAC79" modified="2022-10-12" version="1">MLALENKAISSLRGGGCGSFKMNPRTYESLKSDIQDIENFIAKFNSFVEIICAKAAVAINSIESQEIMIAIQWFIFQEESIYKLNKNTQHIINSYNLIIEGIQKLLKSCLIYIRTDSFKCLYILQITASLSKVIFSFHVMNGDRFMKIDLQKEFLDASDDLKQSMQIEKNELILIQMELYLFLIKTSFQMAPNNRKKREDILNGFVSGIFDSLIQMKPNVELLESLFLSACQIHNMQKIKENRKQYEVYFQIDMLKWEIIQNLKNDSQRDLEEIILQIEKTHEKLVKNSSNWKNHFLWIQMIGKILIYHPIITKEKLKQLTSSFNLEAKSMQIWKEYLKRGFLIQMNHSNDEALTLLNKFKNNELTQIDKLILEETFKGWENLMVIKDFLLNEKNDNIHFTFGSYLKLKLELESMECQKNSSTQTICKIQQFFDHIISNKLIALIQENDERLRVIVYNYKNFIKKNKYFNFTIEQATNNSQLKKIVFNFKEYFQETLKIIKIIKLSKRKQNVYIEQLEQKKINEFNLYQQLKYIMRLLEQIISQAIQDIKDNRNEPDESKMIQQELVLIQKALQNLNLTDQQFSFAQEFKQKILVLSNTSLSQNNFLKEAQFMLTLIQQTEKQVSKLEMFFLQKGNLIQYCQSLLNNLNQHYEHQIQELQKCFNEFLESFKTVLILKNEIESASKPEKFKEIKQQYSRQLLFSFMKKQRLNILKLKLQLIAFKECFKNILTIEFQQLQDNINLDEYLLDVLSIFENSFGNFQFIKKKPPLIQKQIKNNLIYSKNQKTVSNKLDDFQVGNTSLLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPDESKMIQQELVLIQKALQNLNLTDQQFSFAQEFKQKILVLSNTSLSQNNFLKEAQFMLTLIQQTEKQVSKLEMFFLQKGNLIQYCQSLLNNLNQHYEHQIQELQKCFNEFLESFKTVLILKNEIESASKPEKFKEIKQQYSRQLLFSFMKKQRLNILKLKLQLIAFKECFKNILTIEFQQLQDNINLDEYLLDVIKDYPKTIKCCDDSLQDQPIAELTNLQITEQDYQNQLSKQKGVIEYIIFILSLEEQMIDSQSIDLELIENEFGQLFKEESCSFTLTERIMRIIENASIDNSIKTVTNENFNQSELNIEREKYENLWQQLRIFENCDDIENFKKFIGHIEIVVELLRNSQGQNLEILKIPITELLKELKIFKIEFLETNRKKLNFINKQQYQNDQILVSEKNDILENQFDYCEQKNIQTSSDEKYAKYLQNIIMVLKLSSSKIKKDKQQLCQLLEETQQFSNKLHLIQKYNKNIQANTYNKFHSYFQKQIQIFEKQQVTTDNLQQLEKESFQDYFKRIEINILQIKNEKDDKPIQQQTNICNFLNKLMGELKQQLIQTKCVSSKIQFGQEYLIKQKKKYTYGKTQKKKQMIQNQTNHQNYFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M485</accession>
    <name>A0A8S1M485_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0510041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M485"/>
    <dbReference type="OMA" id="GWKECKE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035863396" evidence="2">
        <location>
            <begin position="20"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8073452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="256" mass="30168" checksum="B147A2CB0AC5C13B" modified="2022-10-12" version="1">MRTSVVFIILLIALATTKRIEILNKTQIKYIQKEVIKNEELSQRTSLLTPEEQKKADEAKKAEEAKKAQEAKKAEEAKKAEEAKKAEEAKKAEEAKKAEEAKKAEEAKKAEEAKKKKEEEDKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEELKKKKEQEEKKKKEEEEEEEEEGEKKKNNGQENEQERNKRAEYQECIKDECAQFFKECNDDCVNRLKDCKQKAIYEGLDAYNQCVRNNEKANSLIKCIQSNCQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4A2</accession>
    <name>A0A8S1M4A2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0490252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4A2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="56" mass="6933" checksum="9739688EFA0E594C" modified="2022-10-12" version="1">MVKVANIVIIYLIFVRLFLNQNLVIFLEKWFRVQNFKEQLWYLKQMINANLIVQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4L8</accession>
    <name>A0A8S1M4L8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Golgi apparatus membrane protein TVP23 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0510167</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TVP23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4L8"/>
    <dbReference type="OMA" id="EWIYESQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008564">
        <property type="entry name" value="TVP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019:SF7">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05832">
        <property type="entry name" value="DUF846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8073642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="25411" checksum="D0D013E30AED8B7A" modified="2022-10-12" version="1">MQIADEQQDVQHSIPDLDNVNIKLGDDQKKVNQNSSNTVSQVQQPEQVAIQIQSLPPKTQDASFVSQLFHCLFKGLAIFVFFIPEGLLNLTYCFIIVVILSAIDFWTVKNITGRKLVGLRWWSEVKEDGSEEWIYESQVANFVPNPFNSNIFWFAQFGVVLTWGILILLDLIGLRWFNAVLAMTAFCLTGINFVGFYKCRGEHQKKAKEYMTKIGLKAIQQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5V2</accession>
    <name>A0A8S1M5V2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0500238</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5V2"/>
    <dbReference type="OMA" id="NRNRLDM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8073161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="606" mass="73646" checksum="FC1A1DF650276CC7" modified="2022-10-12" version="1">MYNNASPIRHQIIQGPHNKSVSPVSRFQVMGNIQPSLGMKTILKPKIYEVVEVPLNHYTSVPSFQTPQQQQYDQEFQRKIKQVEQESFICRQKIEQDQRNDQFQQLNEKIRQQQQEIERLKSVNDPNLRLLKEQCDYYFNQSQQAELEKNELILRLKQNEQQMQKIQQQQIHINEKDVKYNQLFNEHEQLKQKYQNMENQLQQQIDKQKAEFDRIHNQNFTQIKQNSELRNKQFDDQIQKLQEELNDAIREVEIWRQKYHKQDLNQMNQTTRINELELINVHQNKEIERLQQQNILKQQENDSLQQKINYLEFNYQQISSSGLDSEVKRLKEQLESRQREIDDLKRRNTEIEQNLLKLKDHEIQIEIIKQRNQSFESQLQESDRFKHQYTSQLIELDELRNKQIRLENCVNDLRRNEYRQQTEINRLNDLIRVKDEEINKLQIDYKKNMTQMTQQMNSFRSLFERDIDTLNINRNRLDMENKELIDQRIQQLIEELNLIKKDREQLQLACSQLTQTKLGLENRIIVLQGEIDRLNQLVKTQNNEINHHLQTLELQNDQIEQFRQQNEIYKKEMDLFKTNSNSNFFQSGSQKKFDFIPNQHSDSNWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8Y9</accession>
    <name>A0A8S1M8Y9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vps72/YL1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0480020</name>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0480022</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8Y9"/>
    <dbReference type="OMA" id="LPTHMKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029525">
        <property type="entry name" value="INO80C/Ies6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200:SF1">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08265">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="6">
        <location>
            <begin position="58"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8071724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8071728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="110" mass="12875" checksum="851BEBA9CE0095D1" modified="2022-10-12" version="1">MSYQFKNSQWQARKKELKSRRQSQSRKFNNIKAQVQINNSAFNYLSIEAPPSLKPAKRYCDVTGFEAKYKDPVTQLYYCDSIVFNYIRNCPKATAETYLNIRGCTQKLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MD07</accession>
    <name>A0A8S1MD07_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0550239</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8076111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MD07"/>
    <dbReference type="OMA" id="INPDHPW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1040:FF:000009">
        <property type="entry name" value="N-acetyltransferase subunit Nat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1010:FF:000009">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021183">
        <property type="entry name" value="NatA_aux_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF2">
        <property type="entry name" value="N(ALPHA)-ACETYLTRANSFERASE 15_16, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12569">
        <property type="entry name" value="NatA_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000422">
        <property type="entry name" value="N-terminal-AcTrfase-A_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="226"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8076111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="789" mass="93594" checksum="17500DE82305480F" modified="2022-10-12" version="1">MRPAQTSLNAKQSGEFKALVKLLDAKDYKKGLRNTEKLLEQVPDNWEVVSLKAIFMYYNDEQQKGLELAKQAVFKNLGSDFCWHIYGLIYKAQKNYIEAVKCFLQAIKKGEENIQLERDTANLQIHTRDFEGNAQLRHKILTKKSNMIVNWIAFIFAQHLSGNYEIAFKAINSAEQLIQKDQQNPIKKVEMNEFKLYQIQLAIDAKDYQKAKQNISAFKNDITDMVAYYELQYDLYMKLGSNQEAVEAAKQLLELQPQNWRYYQLLKKADPQYDLSIYDNNLVQGQLLAEQSGDIFKTNFLKFIDPFFQKSLPSLFREIKHLYKQNEKLNIIREAFESYLNAGPIQKLWALMLLSQHYYQIKQYEKSLELVEEAIAHTPTLHELYLIKAKALHKQEKFKEAYEAADRARQLDLADRYLNNKTIKYALRANMVYLSQELLRMFLRDGSNPYELQMIWFELGVGRTLLRLNYLGPALAQFYLIFKHYQEMYDDQLDFYQFSIRKYTLRSLLQMFDAMDTRYSAKYFIETAGLMIEGLNRLKLKQQDQGKIEQKKLTPKEKKQLQKLQQKQEEEKHLREQFSIEGHIYSIELQKKFDLSGEYLLSQLKTEQDIIKMQNQFAQVLIHTNYSNKEINFQAFKQLVQFYIQQNRPLLSVKLLNKLRNNQTESNQIVNHKYQLMLIQYLNSYKGPQLQYVQEYQQDLNKFDTDFWKSVEIKSNLDKAIKTHCEHILQNKPFNVQQLESNDLEFLQEYPTAKTTNPFYTNDPNLEWQNNQISQFQSTQQTQKVSQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEU5</accession>
    <name>A0A8S1MEU5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SF-assemblin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0560013</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF-assemblin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEU5"/>
    <dbReference type="OMA" id="RERAECH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008374">
        <property type="entry name" value="SF_assemblin/giardin_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06705">
        <property type="entry name" value="SF-assemblin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="164"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8076253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="32025" checksum="ED367314CEAF954D" modified="2022-10-12" version="1">MSASKIFYSPSRSNVNVSNVQPYGGDRALSPSRAKISQLSEKLSNLQHQIDEDQAFKKETFESKIKILEDRATKQAQGDDSKFKLLKEQLQKVEEGAQNEKIIREAGDEKLRSKDLKGLEGFLNKELQSEKINRKDFEGKIIKNTDDKVYSLKLDLARQKKYREETEEKNAQEIGDRILQLQEEVEEERRQREQQNQNTIKRLGDSILKLQEILTTEKKQREQAQSQMFRMLDEMNHYLNSELTSEKNEREATEESLINLIDQTCNRVENSLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEZ9</accession>
    <name>A0A8S1MEZ9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0510296</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEZ9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="645" mass="76002" checksum="9E2DF9215BC0EFE0" modified="2022-10-12" version="1">MIYQKVAFQNQKHKEIKINKWDQIQLQLAKIKIDISYTQDNQILYLHNGEILRNEFLYDNQQDPELLISMEQIKNLQWQGKIEKNKKKMGKWMAVWNGEALIDFCGYYELGLKQGLWKELNKNYWSQAKISESGEYLNNQKIGAWIYLYENKKIGGGSYNSQGVKHGKWIDLCDWFYDYSQIIYQGEYKNGKKIGQWDFYWNWKQKNLQIGGGQYGDDEIKTGRWIELNDGFSWVSQVTYNGEYKNGIKVGRWDIWYKKNYGTNQNIQLQNYMIRQYVQIFSGGGLYDEGNKQGRWIEISDNFDGGQQMTYNGEYKQGKKVSQWDILWNFYHQQYRLMGGGLHDDNEEYGIKIGKWVEISQRFKMSSQITYIGEYKSGSKFGRWNTFWDWNKNFQIGGGQYDNAGKGLKIGKWVDLSDEYEGEQQVIYNGEYQNGQKVGIWDIQYRETVKDSLQNMQNLILCLQIFSGGGLFDEGIKIGRWQDLSDGFRITSQITYDGQYQNGKKVGKWDIYWRHQKENKQIGGGSYDQEGVKVGQWIELIEGFQNAIQITYQGEYKNGNKVGRWQFLWNNNGENKQIGGGSYGEKGDGVKVGKWVDLCEGFWNSKQIAYKGKYQNGKKVDKWVELRREWNQNEFQIVGELKYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MG68</accession>
    <name>A0A8S1MG68_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0560177</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MG68"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="189" mass="22504" checksum="3BE3B9345C263511" modified="2022-10-12" version="1">MIAQEKSIQEKRSNIFYESTKLPKSLPKYIEDRPKSPNLDHRQRMLYQNTSNVLSGYCYQKIKQVCESRIKIVEDPNNRLKHQNQNFSDYLIDIVVIIKQIKELDQFLLNYIGLHMIVLEMQKIVQIMNIQQIIINQNLLSKLLKYPIQKLKCIQQAKELKNYSKLHGKTTSSKEIVYEGMLKWKNNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MH39</accession>
    <name>A0A8S1MH39_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0570154</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MH39"/>
    <dbReference type="OMA" id="YDVEKNY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF3">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="13"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8076983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="277" mass="33420" checksum="0849FD2298C10D94" modified="2022-10-12" version="1">MKEDINQLLKQQNYYDILGVQKNASDDELKKAYRKLALKYHPDKNNDENAQEVFKKISEAYSTLSSPEKRESYTYQQQKPQAHPHQNKYYRHFQPDEEFELFAQSLKRQFQRRAARNETKETTKKSQHNAHYAKLLNSIIKLILLLTFLYFASQAFQFPFKKQPLYQFQKSLQYSVQRTSNKLQVKYFVGDQFKKEKFSQEKLLKFDYDVEKNYVNQLKRQCDATSLKQQNYMRLINKSVYTSEIKRYQKAIDQLDWSSCDVIKSLKLNFEKFDIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJK6</accession>
    <name>A0A8S1MJK6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0610123</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJK6"/>
    <dbReference type="OMA" id="HNGESIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07833">
        <property type="entry name" value="STKc_CDKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000171">
        <property type="entry name" value="Putative cyclin-dependent kinase-like 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001072">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8079102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="441" mass="51146" checksum="D8A77B93B73D7DB6" modified="2022-10-12" version="1">MNKYEVLGVVGEGAYGVVLKCKNKETNEIVAIKKFKETEENEIVKKSIQREVKVLRLLRHNNIVELKEAFKRKGRIYLVFEYVERNLLEVLEASSSGLEPLYIKRIIFQLLKAIYCCHQNDIVHRDIKPENLLISNNHQLKLCDFGFARSLTASTQDLTDYVATRWYRAPELLLSYSNYDKGVDMWAIGCLLCELTDGNPLFPGENEMDQLYLIQKMLGPLTQSQQETFSKNPRFLGMKFPEISKPETLEQRYLCKLPKKAINFVKGLLKMEPAERLTCKQALRHQYFEDLPEAVEFMRELEMQNDQEKRQVSAGVHRTAASPNTQQNVIRTKTSFKVPNYLGNQLNMQNTAYNYNIQGQQHEKDGKQMKKAQSIEKLVPGFKEQKLNMNSDTNKMKVSGQSQFSQQQLQMQQSIKIQNLNIIYNSNTYNQFQKKGALTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MN81</accession>
    <name>A0A8S1MN81_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0660245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MN81"/>
    <dbReference type="OMA" id="QSIFNMF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF95">
        <property type="entry name" value="F-BOX PROTEIN YLR352W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8081938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="888" mass="103393" checksum="E98707A3161361C8" modified="2022-10-12" version="1">MEQQVNQLSWKVQQNPKELDLSYCVFLQDIEFITYLKSPQAKQMQSLNLSHTLLSDASLLQLSFLDSKSLKFLNLSYCVNFTEGAIVNLLNSETIVNVEVLDLSGNQLSDVCLKFVLQPNKMKYLASIILNDIKKGQQGFSYKLFEILSQTNLNKSLLNLSVGNSFVQPDQLQMTKAQTIMNTFFNRIMKEDHTYFYQLSLKGTSIQTLTLFKCELLTNKFIKMLCRNIIFLSSIKVLNIGHCKLLTDKICYYLSEAGASVQKLVDLELLGLNITSDGLDKIIKSKSLKLNHLGLAMCEDIQSDAIKTLMDSKFGKTLTSLEVSGSYSQKSQIVGLFSQEQIIWKDFNLKTIYIELFLDQGCYYQHVGKQQLQFQKQQQLNQNLSQLDPYYKTFFETHMYTIIDQIIFIKKIKVVLKVQYTDEDLIAQYLTNFIEQNNLLDNLLTNDNQLFVNNLCILSYVLQSITKASAKIYDVEERYQQNNQISIIQINYLDPIEKEKINVSQIRNLVLMKQDLLRDEDLALVGRYSETIFSQLSYIQIKGCKQITIKGLQQLGERLLHQLQFLDVRKTQIKLSEYSQILNDFINLTSFNNNKIQLDFNQKNTKDDLVQWLNLLFRLLSLKKLSIKNTNKQSIFNMFLMRFVAQMVKNNHLSMKKLYLQCFLNIADFQLYSEESLTELCESIQLSFNIKTFYLEISGLIKHQFIIDVFNTLINTQITNLNLQLENCNIIDEIFINKQTSIHYFKALEFSTSNLTQLDIILYNTQQLEDLTVVLSLNENKIDSLIYLFQNNQLINLKIELQQAKIEQFLDILIAINPEKIKTAEFMINNILINEIFLQKLQEFLLKCKRLIEFTISMIGCGCTKQMMDKYFKSNLQIFKIPQLKFYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MP96</accession>
    <name>A0A8S1MP96_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FERM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0650217</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ITHCIPL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051640">
        <property type="entry name" value="GRB10-interact_GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445:SF36">
        <property type="entry name" value="FI03272P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445">
        <property type="entry name" value="GRB10 INTERACTING GYF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="2">
        <location>
            <begin position="1468"/>
            <end position="1651"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="565"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1100"/>
            <end position="1172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8081389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1651" mass="197845" checksum="3EF26A9F491813A2" modified="2022-10-12" version="1">MYINTAWMINGTPCWVFIEGNYQRGNIQKLLNQRSEARILLPDDKICDIPVNQVHRASNNKTIDLTELPYLNEPEILFKIKNNVYLDRQVLIQSVNTSLSDYIQYKKYIDERLIINQVPPHIFSSLFYAYINLDLGKSSQLFITGNQGYGKTQIINDLVNFLNKDYMVYYNLIKSLITKKDNHTQGVVNYRFIYKQKQLVGFIVQLHYIDISDYENMQYFQQKWENLPNQEIQKVLIEQQIENGQQYQKFVQQIIDIINGLTCLNDNIQMISILDYPGQTNLISNYISEQIEQIYLQNTFKNCQQLLMQDQLTELYNQIQYEDNKNYLENLEQQNEIPQNLIKLNSIIVSKDIQLLYIRCIKERQDLKQYRILDSIKFYQQSYPHRMTYNEFYQRFHHLDVFNRKITLQKHLNSNQDMKALVREISKRLIQSKGLLFGQNQVYFKKEAYENINKLLCSHNKKLNAFAKIIQKAFRVRQFKMAILSCVKRIKSIDQGKNQKQDSKQQQTENQNQNISQEIQWENMIEISDLDSYQLLNEEQLQQYKKKKVNHLQLKQQSMLDGTVYEEIQNTAFQLLNQEQQIQRENNIKKNKLEQQLKELDAIENEVSDQESFSLLTFEQKLQYLNLKKQEQTQIAYNLLDQNQQHQIKQQKRLKKMKDLQEKIYSQLEPYLSDIEAYKQLTTGQQNEYKRQKLENKQNVAQSLLNEQQQINRIQILINQRPRQMQKKQFLIDDYLSDYESFQLLELEQRKNYVQQKQENLPLRIVQNKVKYGYDIDISDPESFNLLENKEEYLKYKEKLTLFKDISDIEAYLSISDVNNYINQKKEGLNNISKELANPEKYEMLKQQRFRAQQRLDQLNLIRHELLDSNAFRLLNDEQWNIRKQQLLEQKGHEGMINIESIAFQNLSIEKKNQRKNAEQMKKKKKNILNPILFENYLHPKASVAYRLLQNKNTVYKNISLAYSLLKDKNVMDQHLKELIIYPWEEVKSYKDKQELLQQKQSRYEKDKLEFNKVDFSDYEAFLLLDEDQLKEYKNDAYSLLDNEQKQIHRRIMKYKLKSDSQHSDLESYELLSDDSKLKYTEMKIDEISDYCAYEALENKEQYVIEKQNQRKYLNQIEENGFKIYFGQTPKLLTIEEYDKFINNQQQIQKQIEEEENQYLKQQQEEEKIKHQKYDINKQFVIQNLIDNPHPSNFVIYEKAQQNKKLLDIRSTLDKNLNLEYDDELPQILQNNLFKDYCQQVINQQGHQIEKIMKCQTDNIKKPLTKIQNESVALNVFKSITKFAHMKRSSLTTKEHLERILCSLIGTTAQIELPTAPKSLQRSLAVDIDALKKSIELETTSRQFNDEVQFENNQEIREEIFLQIFKQIEGNTKEHIYYLLRLCIVITHCIPLIHPLPFIQYLNEQLQKPENKLFKEPDICKYSKQIIKNLSINTQVKVFKNEIKTQLCQRVYLPSKDELQLMFECKQPIVKLMIHSDTPLWVELEHHFSVQQVIKQACNVINQQNNWKYFGLMIVQSKNESVFNTSYIDESANFFDILNKLEFHEEVNNNVYKLNKRFDIYLRIKQYIKFEENDYDLLDLYFYQLAFDVKRNRYNLKEDELSILQELEIYIKYGGNQINSKYNLKDKVAAKYQYLKIIELSSNTTLENIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPH9</accession>
    <name>A0A8S1MPH9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0690038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8082957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPH9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="134" mass="15267" checksum="DE6FEB4138EFA3FE" modified="2022-10-12" version="1">MNDSKGPKIMSSHSFNSIKNEEPKKAEVIDEQSLQTTQKRGQPKINISRLPPAFANKKPQKKQIEPLAQKKEQKQQVQQNLQNIEAIQEYKQVSQMLSPLKALIINQTLARTQLAIKESISTISLVHERPKENT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSP7</accession>
    <name>A0A8S1MSP7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Plus3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0630094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSP7"/>
    <dbReference type="OMA" id="AKILNMH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.200:FF:000015">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004343">
        <property type="entry name" value="Plus-3_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03126">
        <property type="entry name" value="Plus-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00719">
        <property type="entry name" value="Plus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51360">
        <property type="entry name" value="PLUS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plus3" evidence="3">
        <location>
            <begin position="113"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="51"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="321"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8080103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="447" mass="53453" checksum="185301032BF5F4E0" modified="2022-10-12" version="1">MSKQQNLPYQFEEMTLAQQQMFLKAKTELEKEKAWAEIRKQKEEEEYKRSILLQSSQTQNPQTQKVNLEKISSDSESSDSLQSENEFQKEEPQFEQKIEEKPIMAEPNNEQLDNYFKLIQNAQLKRDQIYYLLMKHYCDNVIEGSFVKINEPNLIRRKEQSYAIAQVLAVVEGEKSYQLEQTQTYKLLKLQFGEVKEAKLRHITLISNQQIQKQEFVVWQKRCEENGIQIPSKEFLQKKERDIIKSTSKLTDDQIKERINYNLTKWIQNPKSNIHFRDLPVYQNFIKNLISTNKSMIEQGEEFIADIQDPGEGETEKKQLKQEETSKILRYQRQNREKAEENMHLNEELKKIEQLLKQFKEHQSQRVEPSKKQPQQIEQQQVIKQNQQNAKFSINHDHQKQLDQKYIRQYPNIKTIEGSEQFKAKILNMHNRELPIDNIIDKMMILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MUZ3</accession>
    <name>A0A8S1MUZ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Laminin EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0690188</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NCIICPP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035936083" description="Laminin EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="3085"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2708"/>
            <end position="2731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2743"/>
            <end position="2763"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2788"/>
            <end position="2810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2852"/>
            <end position="2878"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2899"/>
            <end position="2919"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2925"/>
            <end position="2946"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="4">
        <location>
            <begin position="672"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8083252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3085" mass="357009" checksum="B9CF171349AEB160" modified="2022-10-12" version="1">MHFCRTLSQLVLLSTIYQLIQASCNVFKFITTPETGSVELNLDLTTSIDQDFGVGVWTRFQSQFEDNESLDKKILNYYVFSQLYDGSLPLIYYIKLDREARLIYFEVIIQNQDNLQLRELEVKQEMVGSKWVFFYFCYSNSIKQYNMFIYIDQDVTHVQMASGGEYTNQNDQIIPFILGGSLKYSNLQFNINLDMNSFAGQISDLDLRTGSSNFYPDIPTFLSNLYNTDCQIYTCPATAQTTILANFYNGGQILTKTITFYNKRFVLFGWIKLNELPDIRSLETVFIRATFRKIYYGDKYQGEKALYWKYYQSTMRDDDNGFEVSTYHNDPKSENLKYQTLESDTFLQKSSYFKDAVTFWHWFVYQEGLPNSKIELNIYFGSKKEELSFQMTQDHYERVTYYFNIGGDKFIKGFSGEVRNLTFSYCQLNDDKPQRYCHYSCNTCFGPNENHCIDCVPQAISKRSLNGTYCPCIPNYLDVGEPYCQSKFQVLGGINIQEGLISDTSGCQRNQFLVMMNDNSYCLDCPGAITTYGLYCIDCVYNPTTWYQNPICTIDYLMPMTDSTDYIFTRKDRKQKDYEYYLINKYNNQDKPILEPCFGCFGQTNSTINFIERYTMNQISKILCKNCYTSINGECVNLIPNCDECDNKQQCKICEQNYTLFESQCYQCPIECPNCKYNTTGYYCKSCNEGYYYNSTFEQCQQCGQFCQICFYSNLLNLLQCVKCIDTVDYFIASEFVQCLPKNKPHCKSQVQEITRTFTNETFLSTQYIFSIDLRADLISYPTVDICLKCDPHYINVIIVRKLYGCYSVNEVEGITGMALPLVLKNKVINDANFELGYHEMYTKDGTENYYIMFGDPIIDGNQKYNPIVIRQEKPNQKLCLDKNCEYCIQNYVYTQEYCIKCFKPYYAHKLQGNCIICPPSCIECEFAHKIYKDGWKSDMLPAYQLRGKQELHHFSTYSLSTNVDDYEVNCLLCADDGILHNGKCYPKCPCKQCIIENDEVKCITCENSIKTVINNQCTQCPQFCELCREFTNDEITQINPYFNLQSTTYRQYAKQCLKRDINDPTQGILYNDPSLGQEINCKNPDKKDCYFYVELQQTIYCGKDQYQQNLDLEIDDDAKQYFKKYYQPLENLFSSKNSSHFDIETDFLFNELNVKSVKLVRYIVNILPNSGDCKINKNTFIQSNIRSNVFTVKFLELIIQSQSDIPIYFEGNISFIEFSSVTLKNFQIIPSNQQVIFNVYSLYGMSFLMEHTIIKNAIDLQFQIIIQNPTSIQIKNLEISDSQLININGLISYYFTQKLKNQLVYTINTILIQNCQISNSNLFVQILDTNYGNQKLIATNFKTQKNKYTNSIVFLTDFLNQIRESEFTIQQFVSDREELTNSSLFTLHGAFNVILNDITVSNGIFNANVKLFKLPLFKISNLIIQGNNFQSADNRVITNIVDALYQDNIAISSLTFNSISFKNNYYKGSKVFIELIQSENFKDLRVQIDGLAIESNQFTQKEYSKLMTSTNSSIYLDLTQITILNVNIIRAFNLPEISINNVDKLILKNLKAQLNNQFNMKLIHQQISCLQKNLEIGYGSMLQIFNTKTIEINNLQIIGLIVINLPIITIKSLDGSNYRQSEIIDISQLQFTNNTMILSKLAEQPTILSIISGQKQEINIINVISFNNHHHSYLEDLLFRQSSTILIQNPNSNIILSESTFSNNILTNNQGSNLIIISSTLLVYNCIFKHQNDLQYQYLSNRLIWGYHQDEVAYFENLYSIFTIKSKGGNGYFSANQMQLQNISSQYSLGLQGGAFYFGTQSSGLIKIENSVFNYSQANLLMKDKSQGGTLFIDASQSDLILQIINNSFTNSYSRNEGGTIFIEPSRNNNSIIIQNNIVENVFSLQNAFLKLPVTFSSKSLILIVTITDLSIKNTYNGYLQYLGRIQNLNTQEIKYQTQNYLISIERGNITLKYSTIFNIFDYGAIEILEANYLKLDDLVIQNITIISGSILSLHFNKKYLTSIQIINLKLKDISEVIGDVSLPNKPILTLQELFYKCDLVPIYPTQLQTLYDEQQQFIISLYNFYALIKNKTNPSFIFEIDSISQNHDILIENMNIQKINCTNCQKGLLKFTNIDEQKTQNLIYLRGLNMKDNTCGILSCFVIAAYSTNQIKLFTNSKLHRILDQVTEEFDQQISTIKIEKSLFSNNRADFGGGILISGLSSIISNCQFTNNIATLIAGAIYFDHQYGTSLIVYDSVFANNQAKVGGALYLSEYQINTPQKLNNVFQGNQATYFGNNLADSPTQLTLQIGEKIMQKESLENNSTNKTDIIVIKQYTIGSKQYDYIMLPSGQAIREYQIFDEQTQQYIPYNFTFRIIPLNEEKNQIKALDGSKCFIKGRQIINKSEGEFYSNFTSITEVQFNTTSQDYNLDQMIITFDPDYSQIGYLQLEITCDSIKIPIFSQKPPYQLQEYFTNYRLRVNLQTFSCQRGEYKTRQGTCKLCDSNSDQYNVKAGDQCQIKDSIKMQQVSSARIMLRPEYWRPFESSYKIEYCLNLPENCIGGWNPGNDLCSEAHIGALCEQCDMYNIRGQGSYSVSTPYKCGSCNDIGDNTLKVILVSVWTMISILISVKGTVETVDKMVTQYQMQKFKVFQKDPKAGYGSVLIKVLTNYLQIIGALATFQLQIPSVLQSSVRSVGNPTEAMSFSLDCFLVNLVDIDIIYFRMIWALIMPIIYMLSFLVIYAITIIINLAKPDKTALTTTAIYLFTYLQPTLIGGFISLLSFRQISNLYWIQGNVSYRYDTETHFNWILTFILPSTLVLAFIIPAYMFISLYKQRHNLEKESTRKNWGYLYNEYQAVAYFWEIVKVLEKGFIIIFLTFYEDLIIIKGALVFIIVFIYQLLTRTYRPYKLPFLNLVDEFSTLICGSSIVIGMTIYQSYLSDNQEIIWPFYILLITFNSVFIIIILWEIILAQLDDQQENLDKVRDLINKKFPNLQNSNWIFKRLLTNRGQQQKRIRRRFQMIRRYLIKLVRNNPGIYQLPTPIHSYPDKPVMFENQEKQQLLPNHQQDIKDNTQMVQKRGTKIFPVDQDQKTFESIRYDDKRQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MV30</accession>
    <name>A0A8S1MV30_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0690193</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MV30"/>
    <dbReference type="OMA" id="QGTKYYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="135"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="22980" checksum="C24AA84639D46786" modified="2022-10-12" version="1">MEIEGTKFQEITLLIKSKLVQLEFDNSERKLHEIEKSRAEQDQRVRRLIEDLDYEDRSEWVEYHKTQGTKYYQKQQYEKALFEYYLSILALNDSRMWREFGVALINNIQLNLELLKKPATMELLQFVLYIDTSNIKAYFKLGKFYRSNGQFQTALQYFQQGEKLCQQTQDKESQQDFQKQILDCKRQSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MV36</accession>
    <name>A0A8S1MV36_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Telomere length regulation protein conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0650094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MV36"/>
    <dbReference type="OMA" id="MIIEIKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.720:FF:000022">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051970">
        <property type="entry name" value="TEL2_Regulation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019337">
        <property type="entry name" value="Telomere_length_regulation_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15830">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15830:SF10">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10193">
        <property type="entry name" value="Telomere_reg-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomere length regulation protein conserved" evidence="1">
        <location>
            <begin position="332"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="628" mass="74766" checksum="3B82230D3B7EF576" modified="2022-10-12" version="1">MIIEIKQFLNGENNDIPNKIYEISQTEEGFKFWKNVIFKLRKLNNLQIEKVYNLFETSYIKNFPEISTEEKLTEYLQLCRLCSNFTEGKYIKFEEIYNKLLQQQKVFIYEKLLILGEYELIHESLTEEQISQVKSLYLKKFLKYILLNKLDQYIKYLSLIKQNSQLLLKLALNAKVKQNTESILYDISKQTIIHKDLFDSLILNWSNKNFIKNASFKTQLYCSNLIILFINDGFNNDLIHDGIQKRLNTQANIPRKCGILVWKAFVKSLNLEVEDGFDELNDDEVDEVWNETKKEEIIKQENQTQIIQPTQEFHDFEIDNYENEQPLLLKNLYDCILALRSEDQIRIDQALKHLPYLIRNSLDLHIHGQTLCDILILNRDWKNSQEEMRIKALVALSIMRSQITDHLIEVYFSEEISIMQRIYILKIIRESAIELSSNPNKYQLENYNNFDKSQNDSFYFKQISTTYKTEEQSVIKIRPVPQSRRWGHTKRAYEGLQIVSPYINRFHQMTEHFYFPLLIYSQSKHKLLLTREPLLMLNFLEVLLRMLDCGQNALHISKMVSEAFDFLLIFDKNKNIEIILMKLRIILKITILLKGQFDDRLEKILQSLKYDDPQAKQLYDLILQNLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWK7</accession>
    <name>A0A8S1MWK7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0610198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWK7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="156" mass="18814" checksum="D158A9977A156F8E" modified="2022-10-12" version="1">MYYQINKYKNLIVQYLHHHFVKIIKFQFKCEMKQNGERTIERTNQKKIIRVYKIQNGFISEQLIIKGKIAKMRQVFIILILASICFAQSQLRGRQQFEKQDVSLDEYSFMQFHYHTPPMDLMMVNHELEIQRMMGVPGVVMMNYVIEESPYNPISW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWZ4</accession>
    <name>A0A8S1MWZ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0660034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWZ4"/>
    <dbReference type="OMA" id="ASNQYTQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="243" mass="28837" checksum="31DC4053EDE13FB0" modified="2022-10-12" version="1">MTQNNEELPFNPFNPIKTYRLYSSRSQTAHQPNRQSSVQLVQQQNVASNQYTQMGIHNYALFIRNSENKGLKSDKNFNAYIKKLEENRKLSKNLIQQTKQQQKCESLQTQQFDDDTKKTSHGPQIKNTAKDQKKEKFQHQITEMIAEIKSPFYKKGRYLLNEDKDKEKKLAKPLFFGIQNQTKQQQRRKLVFSQVDINKLRYKIFFDDYNPLSRIVTSQQSVQKEETINESPLKTDQQFFIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0J8</accession>
    <name>A0A8S1N0J8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0680063</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0J8"/>
    <dbReference type="OMA" id="NQQMYNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF89">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="2">
        <location>
            <begin position="49"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8082645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="399" mass="48082" checksum="AC9D6EA3968AEC53" modified="2022-10-12" version="1">MKQHHKQRGYPIDEPVLQAVMSVAQIQNLYPKMSDFNKAINLNSISKKAIFLILRSASLDNIHKGMKYGVWTSTPKSNARIDELFKESEEVYLIYSVVGTKAFQACAKLLGPFDPTATFLYWDEPLKWFGSFQIKCLFLNELKQKTLDEKQPAHLGSIVLTEQTDCTEITHGLGMFVLQCFKDLQEDETNVNILLQQFQNMDRREDQIKQQRDLDQNFLTQQSHEQQLFEKSPFEYKNYHKRQNQQRWQTKQADQMMYMRAYYSSNQQYGGSQYQGQNQQMYNQYRNDNKQYYNNNYDYQFYHPQQQYQQNNTQFKYGYDQQQYNQNPNYYRQPQKYQKKYYNNNQNNYQQNNFQQGNDQQNNYQQNNYQQNNNYSGNNQKKYQKQNDEQSVNNQDKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6G9</accession>
    <name>A0A8S1N6G9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ODAD1 central coiled coil region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0680062</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6G9"/>
    <dbReference type="OMA" id="TWVSTKK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051876">
        <property type="entry name" value="ODA-DC/CCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049258">
        <property type="entry name" value="ODAD1_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21694">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21694:SF18">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21773">
        <property type="entry name" value="ODAD1_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ODAD1 central coiled coil region" evidence="3">
        <location>
            <begin position="85"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="79"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="281"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8082644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="471" mass="55843" checksum="9CF40FD71E683D7D" modified="2022-10-12" version="1">MFFGYNSAPNSNISQTQSILHESQVLSLQRESENYIRKVEQDKKHHYNIEETWVSTKKEWQQKKEQIKKIQHDQTLPAAKQQLNKIKNLQNALEQSILKYNESQTYNLELKKQINMLRKERHQYINIHQDLQEELLNIENETVANEAFRNSNEIQAQKKKETIAEMKKKNDQEKEKYVEQFDRLKKEVMEEKKKHDLDNLGQRKEKAANIDTASTLKLRLKKLISNNKEKVKLIDTYWKNMKVIEDAFNQIKEASGIQDIEEIMNTFIKSEEQNYSLYNYVDILSQQIDQLQDQNQDLKKKIDSQRVENESKKRLLMATPQAERHRKKNELIIKKKQEEINTLRKQMEEIAPTLKDALIELSQTQLASDPTAHLDYKLSFNLNESSLEKYLQDLERFIDLAVAKEKIAQNQSIAQSTLLLDEIPLKEFKGSYKQFNEEELLKQESQQQQYNKLLTQKALRDMALESLKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7L0</accession>
    <name>A0A8S1N7L0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0750185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8086016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="GNECTQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="24"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2462"/>
            <end position="2486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2570"/>
            <end position="2594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2606"/>
            <end position="2629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2641"/>
            <end position="2669"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="159"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="214"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="312"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="566"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="664"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="713"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="757"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="902"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="951"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="999"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1046"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1093"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1138"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1183"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1240"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1278"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1328"/>
            <end position="1365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8086016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2708" mass="310839" checksum="1DC0672F27CB0263" modified="2022-10-12" version="1">MHYIQMAHANSTNAIQNYCTTCDSLKYEIAENGICKCKPEYGLQEGNCVPCTEVNCYDCLDDFQFCILCKPGQNRIVENQQCVCQPGSYDPQNEDQICLACDYSCLNCFGPSNVECTKCLDEDISNRILVYNSCQCKIGYADFQLQESKCGKCHPRCVTCSQAADETTNQYCLTCIPGENRVVSAQLKCECQENYGDNFGTKEVCFICDYTCGSCDDAGPTHCVECLEKSGRYLTASGECLCKTSYFDDQTQNIECSKCHYSCLLCQNSAQSNACLQCPLTREPSDPLATTFECNCASLNSFDDGVSPNCPSCDYTCQTCNGPLSSNCLTCDSTYRQLDLSTCVCLIGYYDIGQLECAKCHYSCYQCFDSTIEGCISCSMDLHFRLLKGNICKCIDGYYDELGNAKCKKCSYKCATCETQSDKCLDCPLNSLRTLDPIKGCFCQGEYYEKENEIACQKCHFKCKACDGQNQNNCVSCDSVANRELKINECKCQPHYFETEVQECAVCSSFCYECINNFDNCTSCANDRYLVGSTCRCITKFDGSEISTFDYNGLVKCQKCHHSCGTCLGIEEGDCLNCIESDHRYQVGNTCVCDEGYFDAGLPVCQKCNYKCKGCLKQIESCISCPDNSFRQFVSGFNRCQCIERYYDDGQNEICQKCHYSCLRCNDIETKCELCSIQSDRIYNDQLFSCDCNIGYYDIGIEKCQKCHYSCLSCNSGDANSCDSCLAMNDSNRVFYINTCKCLFGYFDDGQSPQCQKCDIQCLSCIDHSYQCQSCPQTRKIEANCKCQLGYYDVGLQLCSKCSSKCQTCEISPNNCTSCNSIEFRELNLITQTCDCSIGYLEINGICEQCYQSCKTCIQSINKCNSCVQFRILKNDDCICNDGMYESNSDKQCKLCNKTCLTCANLSNYCLTCSTDNFRYFKSGNTCDCMIGYFENSINQNCEKCASSCLTCSLQYDNCLTCDSNLNLSLVNNKCLCSQSYFFDSMSNSCQQCHFSCLECNNNNECTSCLLTTRHLDVDQKKCICHNGYFETNQKNCSQCHLSCETCENSNTYCLSCINQYHRLLVNNTCQCINGYYEAGIELCQKCNNICKTCQSSANSCLSCYEIEHFRYYQGNKCLCQPGYFEQNTEICLKCSTECLTCQGSADYCTSCDTNSKRFDQSFIHKCPCITGFYSDENNICQKCHIKCQTCINQSDQCLSCNYQVNSNRNSLSEQCNCKYGYFDDGTQLQCQQCNFKCKTCVNDKNNCEICQNSIRINPPKCDCMNGYYEDEQSTCQICAPQCDTCLLEPTNCLTCKPGRIEQDCKCIDGFFETGQALCQQCAFQCVTCDFYQLNCKTCKGDRFQEPQCICQSGYFDDQLNENCQKCDSTCLECDINGCLSCFANRILNQDMECIPPPNSIWYDDTPWCSTCQVAVIKVYLSDDISKIIIHFEFPLNPKGFNSQIEINKCLQLFEVETVLKFGQNSDCYINQKNNQELLISVGEYSKLKIGEEILFKSNSISHINCETTIEKFIFSILKMPINPLPPLIEYNVPHHKLNPFADNSVYLKSIKNNGNRKLDNIIWSCQVKASENSSTLREFLDQINFVQEYNLFIPKNTLPAGAEIQFKIQYDNFILISSYSEFIIYTHIGDLPQININVKPSYFVYETIIIGISAGNLDQQISKDNYKYQIQLFEIDRFPLKSSSSQLNISVQSNSFEQINANIQQYTLSPNSTYTFQVIATNLQTQKFQQQDFTINIPFGGLICQFNNGGIQNIRQDLNLLIYCKDLDTKYDWNDDLDLLINVDCRDLSLNSTCQTGQKQIIKVNTTDTYQFIKRNTISSYSVQEWTVKVTKFQQTQKFVLIIVYLDNDFPVLELEFNKGYLMRKINNFEQLNFTFLIPFDQKPQLLEFSIAIIYNYELIKIHQPKYVSYQFKIFDSIKELNFGDTINLKFLAQYTNSIMPSLYNLKLSINQPPQCSKLFVTRSSDLALTEINVTTTCEQSNDFPYRYQLKLFLRETDLTGFLQGQLDNSLTFHSFQDKNVFSLQIPQSVDSSTVGVLIQVLDSGGSISQIFERITSQPGNINCSQSQYQNNNLQYIVSLLFEAMNQNCSQLYQKIYRDLLYGQILQDSNDNNLKFQAIKLYNQFQQAKIKPKNRLLNEENPLICYDVNTTHYYVTQKSSEIQTNLSQKIIDFQNNIVNLNRTLKYLIQIKKECEDELLRNQYIWNEEIFQQLQYSQDSLLNLLYFLDDIYSDFSKINTKNETIYQIIFDLLNYIPKIVSEIQNTILVNDKPVVVQGDEILWQIKRRTKSDFNKIFQIEATQEDYLLDFIQFEQTSLVSNPLRFTSDLDNQLQQVFNDQTLKIYSQNYNQIKLQNFYKNRFLQFDSFPSTYSTKFGTYQLCLNDTQIIKVYEMQCVLRTISGDFHKCKLNRILKNDTMEQNCECNKFGEIFLTTSYNLSKVIVNDTFEQKQQFDNFYLSQYFLLLLICVSSLILIQLVIYIFYLIKDCKNQQKNSVQSKIDPQINLDKQTLIYIGNSAVFKQKFKQIHQTISFLYYRDQHIQLSYRILEVLSQFNILLCLTIIECYDQINQILIICVLIVANPIIILILRIIYKIVEAIYRFGKIAAAISNFILIILLMAPNVVLLIFYQQKIQIQLGQYQVAIIFFGNIVIWQLLIEPITVFARIIVYRLIASSLKNMEMNSIYHLMHFFVMHSSLEEIFEEFTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEN2</accession>
    <name>A0A8S1NEN2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0740001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEN2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="80"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="122"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="164"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8085174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="272" mass="30032" checksum="57B4622BE975C8E6" modified="2022-10-12" version="1">MKDLSNADLNVLVGLKVDMREHNFENIRIRDTSLICANFLRCNFNGSNFDNVDTSGMNLNQAQLFKCKWKNIKIHELHQLDGHTCCVQSIYFSPNGTILASGGRDNSIRLLNIKTGQENAKLDGHTSNVYSVCFSPDSTTLASSSADNTIRLWDAMTGLENAKLDDHTSDVQSVCFSPDSTISGSADNSISLLDVKTKQQEEKLNAHTSIVYSVCFSPDGSTLASGSYDILSVFGMLKQHNPKPIQIVLLVLLIKSAFLLIELYQHLVKLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGA9</accession>
    <name>A0A8S1NGA9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0800063</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGA9"/>
    <dbReference type="OMA" id="YMIHEMH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF9">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="8"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8088055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="487" mass="55414" checksum="7EE9D55393B53EDF" modified="2022-10-12" version="1">MEIKPKWIFCLLLCTNIIINLDQGIIPAAIQQIEESLELTSEQLGYLGSQVYAGVTLICLFGGKLFLHFNSKMIVMFSYIGMISSLTLFPLNYGSSWPFYIFRFLTGCSKAPMMIYFPVWVDNFGGESKTIWITILQGVIPLGIFVGYSLSSVISSLWQWQAAFYIQVIMLTACATIFIIFVRNQDFDIKKSTKSKLGSKSINDEQSFLAQTSKKSYWQMMKELYSIKLWLCCTIVISILYFIVTGIQFWMTDYMIHEMHQEKQTVNIVFAIVSITAPVFGCITGGLIAQKLGGYQRTKSLYVCVLYCSLCCLSAAPVPFTVTFWFGALCLWFLLFFGGAIVPPLMGIMLSSVPKHLKAFANSSTTMFQNLFGFLPAPSIYGFLMERYNSQVAVFALMYYSFFGLFVMLLAVYFRRQQNNKKVGFEEVFAQQQDSKSSVLQDEENFNITVRVLQYGDAPLVTSLTPHIEDQILNYDNQEEELNQKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGM5</accession>
    <name>A0A8S1NGM5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0890022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGM5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="69" mass="8434" checksum="37921C913D3DD2A1" modified="2022-10-12" version="1">MLQEQISNQQVEQEIQEYEKKAQMDQDEEQHDKKNDNKHQNICEQENKQIYFNIKISDLTLLLSKDDYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJ23</accession>
    <name>A0A8S1NJ23_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0900138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJ23"/>
    <dbReference type="OMA" id="DTVRYKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255">
        <property type="entry name" value="CEREBLON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255:SF3">
        <property type="entry name" value="SULFITE EXPORTER TAUE_SAFE FAMILY PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="52750" checksum="3C6010BC40656B77" modified="2022-10-12" version="1">MSSYSCPSGQYTLFQGTNTLMCDRYSNFCGAFSSCVQVSNAIRPSKLGCCRENYLYPSAANIIVYILIIPIIGIGSLGALGGGVVKRPFLEAVLNFNSGPSGNITACLMFGAQLFNQIIITFQKHPYHPQRPVVNYEVGMIYALAIPLSMQFGEELASYMPLLPVLTMQMMFFIVILPICLLYAKRQEVVEQELDEDYTDSHISMTTAFKGTIQDEAQAALVYKQFLNESHQILPLIPILITFGSFAVNEAVILSRSTLNQNSPYFKTNEQEPNSKLNPCNVWNFYMMILLFAVNILITGTTLLLFRKKEEIKDTVRYKMKERYFTPTRRFFKIYGAGCATGFIAGFLGMAAGLTMFVTMVQFGLVAASAGATANYGYFIVCLQVFISFMVSDSGMSVGDQFFFYGIGIIGVLVFTNLGYYYINKYKIGHILFYIDFAIVVLNIAGNIAWGIEQEDRQGFYSIVNIQNSCNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKS2</accession>
    <name>A0A8S1NKS2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0870088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKS2"/>
    <dbReference type="OMA" id="QIREITH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8091016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="44112" checksum="4178A3618BE42C49" modified="2022-10-12" version="1">MNQISKKIRKTRAKKKCIFESSKIVFCKIFAFLSPKEKLKCRFVCRMFNYFSILSIESLNFQAQNTKMKAFKPFIAKCTNLKYLIYPCLYKRVQMTSLVGKKKRPQIREITHLWFRSYEDIAELMKLNTGLRALELKFDFRGINYQPQISQDVQEILLNSSIEEFIIKEQEIHDLALIELMKHKITCIKLEVKNDQNQLIEKLISIDMKLKKLYIKITIVDNRIVDSLNFFLQQQLDLEVLYLDFKKGVDLQNHKKLKTIILENVCDLKSSMKFPTVLVDKLIIKSLTITHKDIKFLLQNFSHYNTLDIASIQGCWIDILLDKKKAETPVQESVKRLNVYLSKETLKGLQQILTISNIKLFQLEPIENLFNYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NL27</accession>
    <name>A0A8S1NL27_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0870138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NL27"/>
    <dbReference type="OMA" id="YKPAFMY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070224">
        <property type="term" value="F:sulfide:quinone oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070221">
        <property type="term" value="P:sulfide oxidation, using sulfide:quinone oxidoreductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015904">
        <property type="entry name" value="Sulphide_quinone_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632:SF2">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="20"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="420" mass="47469" checksum="4D83AEA9A3764FDA" modified="2022-10-12" version="1">MRNFNHVSLYAFTARAIHSQVAIIGGGTAGLNVSAQLVRDGHYIPQQIRVFEPYKMHAYQTGWTLVGGGMCKADQTMKPMEKVLPKNVCLSDCPVVKIDPEQNTIVTMDGQKYTYDQLIVASGVQRDYASIKGALEYLNNPEAPVGSIYYYRYAQKIDKMIKEFEGGKLIFSEPPTKCEGIPSNIVFLTHERLSARGVKCDFHVYKANDSIFGIPKYSQILGELAHEKGLHCHLKKRLIEVQDHKVIFEDVETKQQYTSDYDFLHIVPPQKPAAFIAESGLGDSDGYVNVHPNTLQHVRYANVWALGDCSSLPTSKTAAAVMAQTPVLIKNLIRTWKLKQPPLPDYEGYTSCPVYTSNKKVLLAEFKYNKQLDETFPVFQSSDSKVMYLMKKHFFPFAYWQLMVRGLWGGRDGIRLFKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NMV3</accession>
    <name>A0A8S1NMV3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0870013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8090903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NMV3"/>
    <dbReference type="OMA" id="APCVTHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051746">
        <property type="entry name" value="Kelch_domain_containing_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260:SF3">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8090903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="63965" checksum="16A22F3713C51593" modified="2022-10-12" version="1">MFNIQNSPNPSELGLGQTFQLQAPCVTHQGQNITNFCKQPSCLMPLCPKCIPQHLDKHRQQGQFADLVTVDEMHEETLQLINTQLTNLNQAIQLLQQFKDSKTDAHGQLHTKLNNAKLRVQQMVDQFFDELLNELDQVTKLDRTNLDVELQQMERSLNLKIQEFQQIDQKLRTPKYLKQIIQLQTTNFLQQTQQQRVECEAFIEEVSKHTLDIQIDEQELYFLKIQLAQYTSIKNVEYYKNQQIQPSSSIPKNTAYNHPYLASEIQTSQIEQSPYKRLEQQFQQTSLSNFTINLPDFYDPLCSQKYLHYFTDKSVFFLNLEWVQNPRWQEVRLNCQVPLKSTSVRSQQGDIFVIGGYIGNQLSSQIFKLDLKQQTLISTDQLQYPRHSSSGVFLQNKLYVLGGQNQNEDLNTIESLEVQMSKVRSIIETRVNLPKMNQGGANLSVCTFRQYVIKATPLEVFDTKLLKWTSFNININRGVGLVAINNSNVLIFGNGQYQLLKMMDSQNLQFTIENLTNPPQIGQVQNGSLIHLGKIYAVTNSCNFIIGTPEKWIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NV03</accession>
    <name>A0A8S1NV03_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1030106</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NV03"/>
    <dbReference type="OMA" id="ARIFFNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="97"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8097187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="423" mass="48706" checksum="EA47534DEAD89F6F" modified="2022-10-12" version="1">MKNCISINQSNSSTLINKVTLYQYGQNPMEISLTQANSLDSLIKLVSRKLSFKKIRLFDHRCQELFEEDLKYLRNESKIYITTNGKSLDGRFVLFEYNPLSVLCKGGQATVMLISHKTTCEERALKFISEGQENQYLYIAREAVILEQLKHRNIVKLYKYKLFPEKSEAVLELEYLKGGSLLDYVLDHGALDEPTARIFFNQILDGISYCHKNNVIHRDLKLDNILLCDTTDLRIKIIDFGLAYLSGTGIPFDEPLNVGTVLYASPEIVFGKLKSVNFSVDIWALGVILYYLVFAEYPFKGENNTDILLNISEGNYNIPKQVSWELISLLTDLFNPDYENRITLKEIKQHKWVKGLIQSNHQLSIPLSEMPTTSKGQQTFENSIRKTKEDILHQIEKQQLSSINSEKLYKRLTPKSCIQHLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NVH4</accession>
    <name>A0A8S1NVH4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0930071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NVH4"/>
    <dbReference type="OMA" id="CKITDEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="560" mass="65345" checksum="DC7824F5361AFA84" modified="2022-10-12" version="1">MRFLIVNGFNASASSAKVFDYFRNNIQNMISKQREVADTQCEYHFRDRHSLDDFLYEPETSLIRMEYGLKFNSIDIVFIIASPNSKPWSPNMRKIVTLIRMCLKTKKLLFSTSFGAQAIAYLCSTNFSVHASITNNHGEGCKLVDFPKYTLQALKNGPNEYFLDSTTGDLYFYCKITDEWLPKANIGLHNRRDAMEYQSIGKYIVKSPTYKPKQNMLENQSEMMCQIKKQFLHHWLFREVTMEFAITSYNQWDIHAITFTNPDKKFNSLAEHNLRGPLIIESDNIIATMFELEPKQKDTVKILSNFIDNAVKLIRFNNNYNNISITNDKYFSGNKGLDNIEIVFQQKRKIGNLNPEDLLKKQQKNFMHEYRKLLNTAIQETERDKIFHVGFSVKKDRLPDFVQQNNIQEKNYKVVRRKSFQMKKAIFLRQQSQINVGGDDDQQMRDLLDEAPKKPLPFQRVISTANNSKRLNSKKHSNPKTPNTIEKEDNEFQDFEIKGINNISVRKMLHPSLDDEFLGSNKIWVPGFLSKDKSKNNHIGQSQRGQRNQNNRFHTDSLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWS5</accession>
    <name>A0A8S1NWS5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1000043</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWS5"/>
    <dbReference type="OMA" id="KEISHAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003956">
        <property type="entry name" value="Transcrpt_fac_NFYB/HAP3_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF9">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00685">
        <property type="entry name" value="NFYB_HAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="3">
        <location>
            <begin position="29"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8096000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="156" mass="18082" checksum="92F2F0FF52AF23E2" modified="2022-10-12" version="1">MSQFRQEDNQKQEIQDDNLIRQIPNYSRFLPIANINRIMKKALPENAKIAKDAKETVQECVSEFISFITSEACEKCKNEKRKTINGEDLLYAINTLGFESYVDILKLYLNKYREAVKAVEGTTGATNQKRKRQNSSDDDEEDDEEDEEDNDDDDQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYM1</accession>
    <name>A0A8S1NYM1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leucine Rich Repeat family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0970075</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYM1"/>
    <dbReference type="OMA" id="AKYNHQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052116">
        <property type="entry name" value="Centro_Cilium_Assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23170:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23170">
        <property type="entry name" value="NY-REN-58 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="217"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="407"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8094873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="585" mass="68353" checksum="A3916938F3B16B36" modified="2022-10-12" version="1">MKLQKALEAQKAPNLILSDQILNEEGCVVVAQFLAQNTQFINIELRGCNINGRGFERICRSISPDARTLIAEWNAIGGGVSALCDLLMNPSYQLQLVDLKNNRIQADHCARICQMLKQNTSLQSLNLKWNEIGEKGAQYLLEGLNGNRNLKFLDVSCNKIPEGLATQIRERIEQNRSSDLMAKNSTGIKESQRNVYQPVKTIPKTAATTNIPDRAVKKNDAIRVAQLKEQIDQELRQNRLMNEELTNRMNALRQEILSKEQAVSMAKRHFEEEVTRKSIIEAEYTQVKRQFDELGKASDYRQQEVMNEYVAKNKHVLALENEYMHELSQIRHDNKQQIDQINANWQPRLDGIQSKFNSLMKANDQYNQQLLRMKEFFLKMRLDHDENVKAVEGRLLIEEKAKYNHQIELLKKKEMETQQTLKRAENESQSIQDDMQRLETNLTRDRQLIEEQIQQEREKVHQTHLQISKRSIKQEKLINNNIVLDHHIDNLIRKIDETFFILNIQKDRGMDDIQQRRLLQERQYALHKTNLQLQHQRILELERLMSSQEGNNIKLRAEYEKLCHIVQGKVQNAVIETCRLNLQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P0C5</accession>
    <name>A0A8S1P0C5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cilia- and flagella-associated protein 58 central coiled coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1020104</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P0C5"/>
    <dbReference type="OMA" id="CKSHITE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049270">
        <property type="entry name" value="CFAP58_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32083:SF0">
        <property type="entry name" value="CILIA AND FLAGELLA-ASSOCIATED PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32083">
        <property type="entry name" value="CILIA AND FLAGELLA-ASSOCIATED PROTEIN 58-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21771">
        <property type="entry name" value="CFAP58_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cilia- and flagella-associated protein 58 central coiled coil" evidence="3">
        <location>
            <begin position="366"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="90"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="468"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="650"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="742"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8096791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="865" mass="103310" checksum="C64840CA8B2A0AC6" modified="2022-10-12" version="1">MQNQVDLDIDTGQFDTMERDFQEVLKDLGTDQNLEKFRSEFDKLYRSLKIIHENQRRLISKCREYNAEISQNASKIQTVLKMTADDSAAIQQLKTQLEKAYKVLEIQQEREEKHKQKIKIQENEIKQLNQTIEQSKALNSGQTTTVHELLQRKQELLKEKEILQIQVFGTRSENQSITDRIKSLETSKESVMKEYKILQQQKSEFEERLQKDEDAKNVTQAELEKIKKDFDSLKEDEKQLGNEKKKLKSEIEKVTLQNSVKTKEIENFKSEKNRVDKELRDTKEKLDSVEKINEQIEEKIKDTRLQIELFETEIAQLHQKINEGEEKKKKMTELVKEQEELEQKIEEEKLLANNGLNQLEDEIQVERKQAQDDRQVIEDLRRARNILQKEIDRCDNNNKKIEEDFIAKQKYLSEKQNELGGLQKKIDYLNKKIANVERETEQQCLQFSQAQTKYFHSLDEIKLKDSLISEFQKKNIETEAKLKQQQNLYETVRSDRNLYSKNYTEKQQEIEKMRRSYKIVNHQISQLKEEIEAKGNALAKEHLEHKKKDKTIEEQSRVLEKYKTDIDEKAEKINKYIKRVDKLQFTIKDEEQQIQNLKEEFELVVAERDILSTQLIRRISETNLLYEKIKINESTLKKGEQQYRERLGDIQMLKEKIADYKREIKVFKREADTIKDLEGDIHNLTKELTEEKLKAKALTEELENPMNVHRWRKLEATDSENYELMTKIHSLQKRLIQKTEEVVIKEKVVEEKEKELKALKDEMKRKPGLEDQAMIPYYQDSLRQKEEQMKAMDQELSMYQSHINEYKLEIDRINKELQRVKQKYFNQKKREQQQRDLRIQEEQGQSIQVILPEKKFVGGGFALQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1Q6</accession>
    <name>A0A8S1P1Q6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0960083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1Q6"/>
    <dbReference type="OMA" id="VITNIMS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="55684" checksum="C3B7E705621A00DF" modified="2022-10-12" version="1">MHKCSLNFEDKKLEDQFQNEKLQKIRKPVYYATFIFFVLNGMKAILEFVRGTSFSSEINVGFVIVQTIMAVMVFKNQNYIKQALVITNIMSGFLQMNFNEEATAKQEFYSFGNSYALFQAIGYFISDFRDGVIQAISHLTIKIIITSIHSKKVDPLCTSLAITSSFFIVLTIYVSDYNYRKQFLANISDDVWDKQLPFLVKKPYIKFTHKNTYFNITSCNLIERFAGYKNDYCFGCNAREFLSETKVNNISLLNKLLSDTITLNTDIEAHLKCFRFNIRVCMYGFEKMNRIVILEKVMTKEKGKQFSFEFRKDLVQTLKQGQQKFQFLLFYNWGIQSCLLINNKYIKQISLMDVISKLNKNYKRYLSPIRIISISKSKLQIRTYSNLIKIYLFQIYHILIITQPRSRDYIDAFVKESEEYIEFQLQLRDVREFCDHYSKNIFVHQIERVILWERVKDDLKIQFSKQISFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P3K2</accession>
    <name>A0A8S1P3K2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1040108</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="EECDIHM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035764333" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="2316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2209"/>
            <end position="2236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8097570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2316" mass="269908" checksum="6D75CE3D0D9BCE7D" modified="2022-10-12" version="1">MQIYLICVLTYQLWIQVNANSQTTQENYKNCINSECTQCKPHYFLVQLQSDNDENYESVSRICVECPYQAYMLDEENLYCGDCLDNSLTWNFNRVCSYDYLTYSTGNSVYHKKNRASIELYYVVQSQPGYYSTMICNGCHHFCKEKSQNCFLIPIEYQNDINNKYVSCKEGYDFDEQRQICISCPQNCHSCIQNTCLICNKGFSKMILRESYEDQTTQTFCIPCFSQCNSCYFGKNDINLNVIPWDAYNNEEGLYIQNFQENQIAFFEKLFSIFQIAQRCEECESTTQIKHIPSLNRRSCVQCGTQCARCEYYSYWQQNKELISPSKNTLRIIEPQNEKETQELLESQYVLRCRECRYYSQLLNAQGTSCFDCKINDCELCHKTESQKQSKDPFTTLNINFEPPPQEDGYIEKCVLCKDGYFLTETGMCLIFDSQNQPDFGCLTFERSYDIKNCRKCQQDYTLYFDQRLQIWKCIIGCDKILQDPHCESCVKQNEEYRCQICKAGYYVDTLTNKCQPCSKDGYCLKCYTLTLNVLHHPEIWFAQYLEDSKHNQVLGPFCYQCIPDDIKIKGRGYILNEDLRKCEKGGDKCKTFEAKGEKGFCDQCLNEHSRSSSPDGNDCIDCPNNTLVCRIRSLEEIQKINQYYKPSQDFEIYSTLALKCSQSQEYMDTKFCRCLENIQNGRQVIINVKADCSYDEQTNDDKVWHINDLYEEKYNKFQNQNYQLDKSLSKNATIILETKAFYNNDENQVSNLNFTQLYIDLNEKGVYKIIINLIFSQSYSSCFFQKDTYFTTNIKKEVFSAQEIELNIQVENDKNINWYLQNKIYFEYFSKVTISGINVQPAVDCQLFPNIKKLQKPYSFQFIKNVGLIVKLENMKILNYAAFQHYSNKNMYTTPYSELSLLNKKFTPFYFIFTNVYDLQLNNVIIQSLNYLQLKDTNYKFNLFGFNYEQNLQLPYFNLTIYNVKFFDIGIGDQSIFDLEFINLKQNLFKIFIQQVSFNDVYFLNGAGFITPKFNNTLNGLVKIDNLFINNTKFNHSRGIVNFNNMQQIYVTNFTLLNSFVNHTVLYYITTIQIAKCYIQNTQFKSKGRMIQTQHELSTILKPDYKRLKFIIEEITFEQVSCLTPQCLISISGIKNDFDIPINITMKNIEIFQIQTDGFDLKILNATTSAAIKIEKSYNVLVQDFQSKENSNLSILYIDQVWNTNIQNIKCHQTKNQMIRNNYCLFISNLYQTIKLKNIYLKNLNAQDNSFIGISSWNNLIYNTTKLQQEESIIIEQVLVQYCTITTTQPGIPSSSIIISSNQQQKVQLSYMQFIWNKHFILIPGSLSPSNPTLIMISSVGNLQLKNSKWMFNTVQGFGAALYLESGFQTITNITMENNNDEYSRGSLDIYQPNQGGHLMIISQNTTIDNCTFYNSTAKEGGSIFIRTLKQGLILIKNTQIKASSTQLDSTIDSKGGCFYVDSKASQLNMTIINTNFEDCVSRQEGGAIYILSHQSQQQVLIQDTSFYNCFSLSGNILKVIYDQNQKQIQQTKMINIDIQANQSKQERFFNQLGVLKELEKYKFLIRTSSIEQDFGEIILINIIQQGQLYQGFLSVKYPSFIKLSSIIVQDQIVIYNPLIEIIEPIINPVLLETVKIANISSISLENIKCNSKLIDWRCKILQIRVDYPQINIQTSLIRFDSITPQTYLTLRNILINKIECQECIYGLVQIIRVSNMLLIELIHIEQFRCTDTHTGYYGCIAITSDYNLKIQTVQDKLYNFKIISNLSREAIINQTNIIPSSDLFQEQNIEQRLLKDEKNYQYIIPDPPYAAHVLIQQLYIENNTCFHGCGLYVYELTTNVTNSYITQNTAKGQGGFIYFLSKDKQRINIAQCNCYNNKASIGGCIAVYGVYTNNLSKTQNQIINNEAILFGNNINSNPQSLIMTINGVEQSIANLSNEKYLILLPVIIRSGYPLLSYKNQNIIIQFLDDENQILKYQEPSILDLQLDSQHLSNQSHNTSLESQNEPSNCEISLFNNYTYREFNNKIQGFNISDLIICYDPNNQRQINITFKSTKIQQPTYIDEYPYSFRKFQINEYQLTVKFLPILCEFGEYYAEQQFKCIKCSQNFYTLELNSNYCKEKDWVKMKNTYENKIQLFDNYWRPEINNDQVESCQKQKCIGNYQVGNELCNLGSIGALCEECDIHMIFWEQSYFKNWNSDCQLCKDPQIYLLNIFLILIQIPILLLLGHMSIAIRIRNIMLNFLESFKSSKIEQNQQMYPQVLLIIIYHMQTLSFLRSNNLDNTQYSNFIAFNSISMPALNLKMIFIQFQSIHLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P603</accession>
    <name>A0A8S1P603_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1070043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P603"/>
    <dbReference type="OMA" id="WENSQHG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPRY" evidence="1">
        <location>
            <begin position="239"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="356" mass="41642" checksum="1498047DCB840B36" modified="2022-10-12" version="1">MIQSCKCDDGGQKIIGVCTALNCQSQRASCVECLKQLHLQHANKFLDLKQLSVFQEDKKKTFSYLKKFYNDFNRLKSILDNFIVSLNIEAYEKNIENQTISQLNSLTDKLVKLNYIEQSGYLIKSFQNLIQNMEDAKNNIQRLKFEYTLNEFISPSQNICLSVNSSQLQMQIILSLYRQQQNKNTLVIQEKIRFSQQYKTDNIILKDQETIVSTTDDKFGWVLCEPGIIQNQFVKNFAFEIIKNDWLAIGICHKDQFLKQKGNFDIWDNNKHGCYLIDQSGKVFSQLKIEENNKSQNFKINNQDIIIIIVDKTSKKIQWINQTQNTQIWIMEFDASQDFYPVVCLRKSSIKVIDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P667</accession>
    <name>A0A8S1P667_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Target of rapamycin complex subunit LST8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1070066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P667"/>
    <dbReference type="OMA" id="VQRNYKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001168">
        <property type="entry name" value="G beta-like protein GBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037588">
        <property type="entry name" value="MLST8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842">
        <property type="entry name" value="G BETA-LIKE PROTEIN GBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842:SF0">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX SUBUNIT LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="75"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="206"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="258"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="36249" checksum="C094C7008BCCF3ED" modified="2022-10-12" version="1">MSENDVILVTSSYDHTFIFWDATTGQQKEDINYGEKVIVNRIDISQDKKHLAAGGNFFACYYDVISQKKVPVYVYDGYKNNITGIGFLKDSNFFYTCSEDGFIRIHDLRSNNLSREYQEKEPLNTICLHPNETELIVGDQMGNVKIYDFQQNKVRLKLSPCPEVGIRSISIAYNASQIVAADSQGQCHTYMMNNMDLTEQSLLYKFEAHQDYILKASISADLRYLATCSADKTVKLWTLNEKNLGNDKYPRWELFSTLYGHGKWVWDCAFSCDSEYIITASSDLTTKIWQTDSAAVLRTLKGHKQAVTSLSINDIAID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7F9</accession>
    <name>A0A8S1P7F9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1080101</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7F9"/>
    <dbReference type="OMA" id="DSWVMIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="41"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="462"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="759"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8098966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="884" mass="102842" checksum="66CDA1D7D90D6DDB" modified="2022-10-12" version="1">MIGDIEQKGISIMIENYSEYQLNIYYLLFCMNQYERVLKDSWVMIMITLLIMFQSIFGNGSLIITITIVGIQILRIILGFISQTQKYLIHFEILQQWTLLVALYLSSKFNAMTILALCSLKEFFFIKINGYLPSLNKIQITHQIVSDIILFTMILLEDYNSVYLSTTAIVVHWLILFHNSKQKIIRRNIDSSTQQEIHDKIMIDSAGNTQNHSRLKLQERPSHHESPRHERDNKMEILSIFPQGIGLIKITDQKIELEYHNENLLKLSMAKQSDQILSSLFCLEQQSVQMDQEQQQLDYKNSISSFSTFQQTPIPCNLINKASRNDKRSLTNKLQSSKKLELYRQNSVLSNIEYKGVILKKCDTIEESILEQELKLISSQMRKIDFQNQMKNQNELIQEHVVILGYQKGSDGKKNRTIEVKLYNALIIDVPYILMLIRDITHRDYIQQLRDYSKQKSKTLSFVSHEYRTPLNCIIDMLEQGVDENQKYSSYTMIMEKEVRRSTKTNIPIKMAKQIKIALDHAKYLQNLSDDLLDLAQIKVGKFKINKAKFNFSQLLETCVDLFQVIAEKKQIMLYINYENKAPRYIYSDSSRLKQIMINLLGNAFKFTDEGSVTIKVTQINLRLEVSVVDTGIGMTEEDQLRIFQAFGKGNSEEHKKMNKSGVGLGLLISNQILQNLNQDLQNGLKFKSQYKKGSTFYFLIQYQDINEIKSLNSLEERNQDSEESIIIQQQQEESIHKVYVNNQASIFKRSTKAIMTTQIMIVDDVCMNIDMLKRRLAQLGQDQVDSVTNGYLAIEKCQKKWQANQDYYKLIFMDLEMPTINGIQTTKKILELSQQYRIDVSIIGCSAYESSEQKLECLNAGMKDYITKPIQLQDLKRIMQLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7L7</accession>
    <name>A0A8S1P7L7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1080136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8099036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7L7"/>
    <dbReference type="OMA" id="HEKPQEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052842">
        <property type="entry name" value="ER_Co-chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45184:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_102, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45184">
        <property type="entry name" value="DNAJ PROTEIN ERDJ3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="19"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8099036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="450" mass="54558" checksum="6AE0A30F7BAAA225" modified="2022-10-12" version="1">MLRLFYKLRQFQFSTTQFDPYVELGLNSTATIQEIKDAYIRLSKQYHPDINQSHDSHEKFKRIAEAYNILKKAKFEEQLKEEFKQYYYSAQSEEQLFQEVFGFYFHEKPQEYYKPENAQKRHQYQEMLKKFKQQKNSTYDHQTDSKYQKQDFSDIKINRKFEGHQGTKTNPQQIYTLIAFCSIAMGGVAFYITMLRKAPELQKEVINNQQIKILRQQTQLRHQQDIDNIKQMYSNINHTMLLFDPKCNSLDMLFNYTIFPDLEFYYVSPELFYQDEKYFLKSDQFEKNAMRKSKLNYRITHAIEAERCLQNIFVPVEEVLNQIEKNGEYTPPGIFMQFYGYIYFKIRRKPIIVENLIKSKEYISLFDLYFYYQTPVPYYYDTLNEFQIFNDAGNTLSHWEGNVYFSTKSLDRFYLKGNVKNIFMQRPQQDSNTEQMYMLAWGNHFEHFQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P851</accession>
    <name>A0A8S1P851_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1090086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P851"/>
    <dbReference type="OMA" id="YENRCPL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052356">
        <property type="entry name" value="Thiol_S-MT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45036:SF1">
        <property type="entry name" value="METHYLTRANSFERASE LIKE 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45036">
        <property type="entry name" value="METHYLTRANSFERASE LIKE 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="84"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="185" mass="21165" checksum="C2C705E3C14D9BD8" modified="2022-10-12" version="1">MIGRFVGASVALFGVAAYGSYKINEAKQFKELYFDTLPDKRQYLLQQHQNAAYVYDPTFDSYEQTSKIQSYRKTIVGLCSGKILETGVGTSRNLKYYPQGSDVTAVDWSSNVLEVALLKSATNINISYRLEDVENMSFKDNTFDTILDTFGLEYYLNPEKAISEMKRVCKPGRQDIITYIWQKHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PAR3</accession>
    <name>A0A8S1PAR3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1110071</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8099984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PAR3"/>
    <dbReference type="OMA" id="ESMWKFA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF0">
        <property type="entry name" value="LD18904P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005225">
        <property type="entry name" value="LAG1_LAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="114"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8099984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="351" mass="41782" checksum="7A6CF989A2DFC5EA" modified="2022-10-12" version="1">MISNIFKPKKQQLIQEGQQNITLGKLVKFSHYETIFKYSFFAVLFVISLYSLDVIQFQQFLKEKNLRRFDWSDLKWMIIGLIMIHITKEINRYLVFDYVSKSLDQKYVGLDRQLRVQKIIKWIYDTFYYSIATIFAYIVFKDEKWFPTELGGSNFTETMYDFPNMPDNPWVPFYYMIQASSHVHALLLLMVYGTKIELKYWEYLLHHFLAVSLLYFSTIYNCESIGIVVLVLHDISDIFLAYGRTYADIGKNKILVYVSFSAIQISWLYTRVYVFPIKIYDIIVNHPGFSLYWENTKHALFNQVGLMIVLFGMHIYWTIFMVKVGIGIFSAGKYKNVYDNRENKLDNKKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PFA6</accession>
    <name>A0A8S1PFA6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCR4-NOT transcription complex subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1140129</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNOT11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8101138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PFA6"/>
    <dbReference type="OMA" id="NDITQFN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019312">
        <property type="entry name" value="CNOT11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15975">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15975:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10155">
        <property type="entry name" value="CNOT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8101138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="309" mass="36311" checksum="9EF8B0AD1C0181D6" modified="2022-10-12" version="1">MQANTKVPVDIHKTLEDNFKAIKEQGINEICNFMLSSLREQDLPQYIRLQYLYNVRQINQKMSLTELCNNQLELNIVNNIKYYGQDLDQLLNQMDTSLIPSCEYLRPIPQILTPQWEEISPQFIHQPIWDYNINPFLFRFLLAQAQSSKLPPQDQQMILTLFKSEYKIDITQFNNLVEHNPQLVSDIITKLHQSGVNVHEYLDFLIQIKVTIQTLELVNQLTKNITLPDQFLNMFITRCIVTCEEVKQNQQQLARQVRLVSVFIKTLIKQKTFNPKKIYVELQGFCLEFSSIQEATQLFKAIKNAVQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHP9</accession>
    <name>A0A8S1PHP9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Katanin p80 subunit C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1190024</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHP9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008352">
        <property type="term" value="C:katanin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002636">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028021">
        <property type="entry name" value="Katanin_C-terminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19845">
        <property type="entry name" value="KATANIN P80 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19845:SF0">
        <property type="entry name" value="KATANIN P80 WD40 REPEAT-CONTAINING SUBUNIT B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13925">
        <property type="entry name" value="Katanin_con80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="104"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="147"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="189"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Katanin p80 subunit C-terminal" evidence="5">
        <location>
            <begin position="459"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8102675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="633" mass="72895" checksum="0EDC533996491BC1" modified="2022-10-12" version="1">MNRNLKLGEIENLSKIKLARFSETGTRFFTYVDDLNQIYINKIENSKNFLDFKLFNPFQKYFAQSQNEISSIIFSSSENEIINGSSKGIITFVDISTQKIQTNIKGHLSSITSLAIFPNQEHIVLSGAMDSQVKLWDSRSKTTGFTLKAHTLSISALVVSPDGKLLASGSNDGSVKIWDIHQQKLLATFNESDSSITCLQFNPLDKAIATASDDGCIKYWDLDKYNQISSTKPDKQTVYSIRFCNEGKSLISAQNFSFKFWDLERDALLLDNVESNCKGILDTYVFDDRELYSLSTQNQTGLSVFGSKLSNINFNSRYSIDRHKQRIHSERIQSCEITDQFQQRNSVNILKPKNSQRIFSNNVNLKPQNQNLIEEQGFQQCNNNNGNKFVPTTQIPQMVSYESVMQEEEIKENQTINQEGQSILDLVTSQDEVNLSSFVIDDENKLKFIQIDLINEIEKDHNRVKQILKQRINYMKPIIYWWSNNNLKSAINAINQLVEPSILFDALSMCSHSSKFKMIPIEQMPQLLEKCKILIESKYLSHIKGGIVFSYKTFSMYRDDILTIKCFNQMSKVDLSREERVAKYDKIVEQLKQIIQMSKITKLIERNKEEISDLAKKFQIEMLGMLKRINQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIH0</accession>
    <name>A0A8S1PIH0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1190071</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIH0"/>
    <dbReference type="OMA" id="ECTQEAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="137"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="139"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8102769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="230" mass="27556" checksum="FDAB39D740CC7980" modified="2022-10-12" version="1">MHFQTFLEEPNKLTSYKQLQQQINEQKEQKVKSDIQNLEHIWVQWLSQQQLSQKQNDDTLKKNESIDIECTQEANAVEKSTQLKNFFEILKSRIINEKLYSTMTQTQMTQFFEEATTQIQNKIKLYEDIQKLKLLQNYPSNTKRTYSKSANMTLKKWLIENYNNPYPKPHQVEQLVQQTNLTNKQVLNWFINARNSLKNKSADEKKFKHIVECKFKELAMMKKKKIEQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PK63</accession>
    <name>A0A8S1PK63_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SIT4 phosphatase-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1210099</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PK63"/>
    <dbReference type="OMA" id="MINYIVE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF8">
        <property type="entry name" value="FIERY MOUNTAIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="706"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="830"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="215"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="731"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="757"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="799"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="835"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8103540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="849" mass="99532" checksum="FEEB80EB9A38CF14" modified="2022-10-12" version="1">MVNNNNKKSNGKTKSYQEVCKNSQKKDYKKINWSKTHKNYLSNSCLSISYHITKCWSKKITKIKQKKQISQRKIKQKSIKKVNEIQLINIQQYNSQMFFNFGRFFGFGQQPKLDNLLKKENLTIECIFNEDDILQELKGTSSSKFADFLIQHPQEYLKMINYIVEEIPDQCMEKNRCIKYPFYGSEILGSENEKLINFLFEKPSEQPSDDVRNPIEDEETEQVEQLEQNNNAQENEIIRAGLLDNLLKLLESDAIIITTAGYFAKVVNAIIHKKGHCFWEHLKHYPDILSNLFKHAYLKHIVDIFEKLIILEENFEQSIEYLNERSALLQRLVVFLKGKQHSQVIVGNICELFVELYKKSINQFDTQNQELKNMLAQFTITTTPLFFMNLALITQQSVVYNVLNVQFEFVNKYYLSDQQHDVNINLIQLYKPVIQLLEKALKQVDSFKIPFISTNGDEIIPLGDAKLLIIQLIIQLVNKSEFYSYFTAELFSQVIDLVKLYPTNNQLHLLFERLIVTLVETNDEQLHRLIFEDTNLLLFIIQNNDEKARQNKSGFQGVLTRLSNYLDCNKNKSNSFQVSSQLLTTISMDWDEYMKGLAIVNKIEQEWILGINPKQKEQKFVEEIISPNVKEETQEQNHVIQGIRKCISSDNNDVVQPRDVDPEGVEIEEDIQLDSDEKLNEDGNIEEQQIQQQDSEKQKEFEEEVVNQNIENNQGDQDINMVDDENQEQQLETKELDEKIVDEQRNEEQQNNSIDNQEIQHQEQQNEVVVAEQDIQEETQKLEQTEETQEIITENAELKEDEGEKEEIEEEIACSQDSLEEQLIKNAEEQQVQKIEQENENKEEPKQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKC8</accession>
    <name>A0A8S1PKC8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1210177</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKC8"/>
    <dbReference type="OMA" id="SIPKFLW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="331" mass="40468" checksum="3AEE8E6D6CE6FDFD" modified="2022-10-12" version="1">MLMQNQQGLYQTRMSISSNRSNDIEFDKYKQIIETEISNNWLGFKYLNQLEDRKIQIVNKISEYLQKLTNGKQNLIIYCDFKFSQNLDLLIKTSDIRQQFLDQQPPNENMGVKINQPDLYSALIKLREFMFNECLKFKEWLKQNQDCKGDIWNQAEWFLQNNGEKESNKYYVQAEKHRKNMMNQLNFIQENQDYYIELSVYLDHYYEYFDRLDVSQRTKIYQDFIQFLDQIKQQHEIRYKYNQYYKNNTITDRNQKQYIVNQPLQSKYQISIPKFLWYEIQKADLQGYILCKMIDSQELNLNYKVIENIFLNQRDKVINSQTHKPIAQMYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PML7</accession>
    <name>A0A8S1PML7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHY-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1240041</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PML7"/>
    <dbReference type="OMA" id="MKCIHNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="4">
        <location>
            <begin position="524"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="341"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="451"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="297"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="388"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="500"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8104507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="604" mass="70112" checksum="D9DC65A557A7B8A5" modified="2022-10-12" version="1">MSDSKNTPRNHGRSKTKTSENLQIQFKKPSVANERLVVNKLGMKFDPVCWKKNHEKGKIQFVCIESNCNADRRLACAHCIIEEHDEHISNKVTVEQYCDTFENNFKSYLFQVAKLEEYYKKCKMMDLIDQIDKDINTVVEIVKQQLKALRDYYVQDIQLHAEQNNSSLFTFYNTERQTLSKIKDYAHKNLFEMTPAEMDHSMLLMGQSHLDKILIKLSEFDDQTSTLQSTMKSQWKLIYEQLEESLIDIIAQIGQNNLDFIQPIIKESQQEQYQSTNQQQNLDIQKFNLQNVKTSTKQQQINQQQQQQQQQQQQQQQQQQINQQQQQQNNSLQAYITQQLPPQPQQYSQNSQSIKKEVSRHSGNTVQQQSSKQQTSNQPSSSNQQLSHKQLQSNSPRNSSDNQSFKQIQGICQYQVISPLKCGVNTASFGNLGSSNQKSLKNITQTNNNQVPIQSQTEQKNNSNAKPSSHIRQTSPNGVNYYYQSDGKQYENRKNVNHVQSLNSSSQQQQQQQSFERRLSAQKVQSTQFPCNYNNMKCIHNELIKASPIFSCCNWAFPCYECHDMISSHKAHITVPSQRFCQNCHEIFSVNLMSTVDVKCYKCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PS36</accession>
    <name>A0A8S1PS36_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1280117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PS36"/>
    <dbReference type="OMA" id="RYLIKME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="10193" checksum="5741CAE6498756F2" modified="2022-10-12" version="1">MESRSYNFSTAKKRSTQMESKVIFFPREDENAPRTIILKQECPGELAAFKECVVRTKDESKCMQFKDALLECGKPAFRKANTTPDYQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PWY2</accession>
    <name>A0A8S1PWY2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein MAK16 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1340129</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the MAK16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PWY2"/>
    <dbReference type="OMA" id="DKGQNFC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.110:FF:000001">
        <property type="entry name" value="Protein MAK16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006958">
        <property type="entry name" value="Mak16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029004">
        <property type="entry name" value="Ribosomal_eL28/Mak16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405">
        <property type="entry name" value="MAINTENANCE OF KILLER 16 MAK16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405:SF4">
        <property type="entry name" value="PROTEIN MAK16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04874">
        <property type="entry name" value="Mak16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01778">
        <property type="entry name" value="Ribosomal_L28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003352">
        <property type="entry name" value="MAK16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal eL28/Mak16" evidence="6">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="190"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="194"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="279"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR003352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8107725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="288" mass="34731" checksum="CF36B638193665E7" modified="2022-10-12" version="1">MQIDEVVWQVINRGHCSFKIKTISQNFCRNEYNVTGLCSKQSCPLANSQYATIKEEKGLCYLYVKTAERAQKPKELWEKILLSKNYEQALAQIDEQLKYWSNFMIHKNKQRLTKLRQMLIRIRKMRLKGFKELIPIKKKAERRDKIREQKALVAANLENAIEQELIDRLKNGVYNEIMNYNTKAFEKVVGQNQMEDEEEREQQELEEEEEDYSDEELIFDPNDLEEDDDEEDQDDEEDDKRLFQKQTFNQLRRENDEVKDIKKKKPRVQLQYEEEEEVEDRKKQQIAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PY12</accession>
    <name>A0A8S1PY12_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1330154</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KSEWNKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF4">
        <property type="entry name" value="RYR_IP3R HOMOLOGY ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1711"/>
            <end position="1734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1770"/>
            <end position="1797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1812"/>
            <end position="1832"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1900"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="1680"/>
            <end position="1934"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="601"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8107409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2020" mass="239926" checksum="0E16317924F38D47" modified="2022-10-12" version="1">MLSSRRSQQPEFNQIFKSQECNFSANKMIIQEMYQCGQCFDYPHGICKYCYEHCHKKTRNVYHQQAKLIKIRGNFKCECGLFKHKQKQLNIQSCPLNSSIFCPIQNCAICQTNLCSFCIKNCKHQHKYIPGEGCKCNQLHSKYQLRLHYIEQLKKYNITKTLKYKPEDELIMIKITNALLNNFDIFEILFGELKNTFENIFNELETGDFNQTCIWSQKQIDVQQDEQNLNEKILELFTEKRFPYTEVASILVFIEQLLIKLKYNLIYLDDKIWQCMTSSQIKFLVSQYHILSQNLQESAKEKLSQLVLKILKIQSLQLKYQFSKFRKIDTLDIENSTPIQRNQIRKYNQCLDNGILQVELTELIKRYYRILFDIEITNPILSKLLIVLLKCVKMGLQTGLLIKTQVIFKVILNLEKLCKDIDYYNQQKSFGQIQRFFTEQMFNLLSKILILTYFQMKDDIFYEILNEQISRNEKRQISLIDKYTASFYFEKKKKNLQIDLFYNGESEKLQIIWRVFINIVINYNLNGQYYNQMMNPQTKLNHIIQIMLRENLFVDTTLRTQIKQLQIEDLLLWKYEKLTILNQDFIIQLNNFKSEWNKYLKNSIQNSFEFDETELERLENLCKDLLNTKYFKELEKFEFNQEKMCENQKKTQSAKNLQDELKQHLKQDMKIIKQTQQILYEKSIDQIIIRYCQFLIQNQFVKLTQLEIIINITNLMFQILKRMINYNQQLINQIYTVENYQLFQRIAEKELPYYGQCKLRNIIIQFHFDVILKSGFIIVYQYCFLNPQFLLQHLINLNEILDSQYDDCAELTQKHASNILYNKHQIFELIIAILELIIISNEMNHDYLQFHQICENLYNNILPQVLNHSTDIFTKGNQYTPNSSSYDIYMNQNYNRYSFMSFNNSLEYIQNGISNNQNSPKNQLSQMSFYSINSLNQSQIIQQSFTMKQLNEIFQKQKDISNQEELAIATILLTHTFIRFMNLIIDYFNRNQLLETQDLKEQALIIEKIELIHQYFGQQLEQCQLFQGPLGGFEELVQVMEKFSSLVSYSLSNKLEKTKPPEIFQIKENNLSVAISQKAIDDIVRFKDKNIFQSLNKVLLKFKLKRIELENDILEQNAIVQLIKKEYHNRSKEQYSLIFINVLIELLQQQTCFQRNRNLVYPNTIFMSHINYIIQLQRLLQAETKPVQQIINTNIEKQNQKYPNFQKNFDSFLEYLSTKIIPYLFKKVIVYQDQHYLSIVINSGKYSIIVLEFIRYLAEGQNSEMQKYVGESGLLLCILQLLVDLFKSKQILEIVRETSNQNKIKSQTKASFQKAVHNVRSMQLKKKNSSTTDYLLKFLQKRKNIQKQTSTQNEFMQFGIHSTQGDQYGIDVSQAFLKLFAHTIITIAEFNQGSQQNNQKLTFNELSKNRVWQVIIQILIVRTNENQQNSVYTLRLYIFQLILSLLEDPERNSDDFQYIMNLINHKLLEETLTQTYRALIIFNKLDLKDLSTIDSQLTEKYRRELGDNINESLIFDSKMLIKILYQIYKMLNLYATSSLEISQYLKDQKQQCQQSQTQLLKREGDHLVKSAFQFIFNIVGSIEYVNQKDQIEIFHFLKPPMCFFLSEETKLQFLIETIDRSNSASKLASIFDSIPQFMTEMKENMSKYRKSLFFQRLINVAISPNFNHTQLATSITALNIIVILLISNTAFVKIENDENGITMKYQEPHSLALTLCQILLIFTSLLALICQLFFRMSIYYQISCKEVKHTFYNTFVIMFKILQRRDIFQFFLHFVLSILGTFEPFCFYLQSFCFIYISQTMSSVLEALRLRWQQFLGIFSVLLIILNAYSYIGFRRFSTSFQLDVIVHPENDIHEEELLCDTPAHCFISLVYFGLREGSGLAAHGDIESYHKSSDYYPRFLFDFSFFIIVTLIMINIINGIIIDTFAELRDKQQQNEYDRNNVCVICSLERWEFEKQGLQFQEHVDEVHSLSNYIAFIVHLLQVGKQNMNGIETHIYTKIITRDMSWTPLKKTQQLLDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZ07</accession>
    <name>A0A8S1PZ07_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1350040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZ07"/>
    <dbReference type="OMA" id="EYLDCKL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="78" mass="9195" checksum="DC73B3D5534C4AC4" modified="2022-10-12" version="1">MQGLHQLKLQTQSHFQYPDTPQHIKTRPLPSPQPEEIETFSLDDSWNSNISPTNKKTFKIDKPILVIEYLDCKLRDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZ56</accession>
    <name>A0A8S1PZ56_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1350011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZ56"/>
    <dbReference type="OMA" id="NMQESIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="390" mass="45874" checksum="B9AFC288EC670236" modified="2022-10-12" version="1">MIYNNIIIFVIQQMHKQLNSISFLSANSGFSDLQSCSIIKGNNYYIAKSIDQHPELIQQLFETEKLQKTKNIFQDPVNLKILQHDPAIGPKYEKNKIVSRSIVGKPDIFRKKQPIQNNHSNQDFHMPTQQINMQESISLAKKESPLQRRLTQLKIAQSPVKISKQTKNENVITGSELISKIAQLEKRLIQNKLDEQDQLDNMPFLKKQYLYRDQRALEDFDKQEKIHSELLLSLSARVSRNPSCSVMMDLYNFRRKQEYSSTNDKMRQLSENKNQEIIEMQEQNQIPFFICSDRQQIIRKPHIFEKDATISNILKHMNEDKTFQSFTTNNFLKEKEQKYKLLAKDKIKLNIDELMVVGQSKYEQEISIDPSHKYVIKEIEKHQGNEEEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q019</accession>
    <name>A0A8S1Q019_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1380089</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q019"/>
    <dbReference type="OMA" id="KNSNYPY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF147">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="452"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="453"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="467"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8108850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="580" mass="68018" checksum="78F93CCCEFC5A88E" modified="2022-10-12" version="1">MQQDDSQTQKEVTKPKNKVKKSKEQLIINVSSSQYPVIRYVGKKMLNWKIQKDENATNWDVWWTDGAVFSDLLGRMNSYQKVNHFPGMYSLARKNHLGRNLMKMHKQFPQAYNYFPYTWLLPAELSDFRANIGKNRTFIIKPEASCQGKGIMLVKDAEGLSIHEHYVAQRYLSKPYLIDGLKFDLRIYVLLAGCDPLRIYIFKEGLARFATELYKKPGKDNLDNICMHLTNYAVNKDNENFVFNQSDKQMDVGHKRSMTSVFELLKSRGENVDQLWKTIKKMMIKTFCAVQPILAHQYKSCQPNNHMNNMCFEVLGMDVILDHKLKPYLLEVNHSPSFTTDTPLDATIKRELISQSLILMNCTQKAKQECINQEKQLLQQRMLTTKPIKLTLEEKQKLIKQCQEIRDQYENQNIGNFEKVYPLEQYEEDYDKFIEHAGLLYQESTGANIKKIQQQQQQQQQQQQSFKRIEKPEMKSECTLRPQEYRSETKILEQRTEIVRLKSEKSSGMKRILKKEHSTTLLADDTIRNIRLASLKPQQRAILKQDTVRKNFKLPVSQGTFVTPKLFMITENNKNSNYPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q0Y5</accession>
    <name>A0A8S1Q0Y5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1380094</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="RTWICIL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="988"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1075"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1143"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1174"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1206"/>
            <end position="1227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8108861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1283" mass="149397" checksum="759C4FF2CE15985D" modified="2022-10-12" version="1">MFWFTTILFKISYQQILFYSAFTDTTLTDIDNWSFIGTDPLNVFQFCGPDSIVGGFQKFGVGIAATKFLLLDPHYKMKISFTLFIIDTWDPNEYYHVYVDENLEFHQTYNFYEGQAYICGQIGNHRQDSIHEISFEFQHTGSTTFIELTSTLDQDAFDESWGYRNFRLFLYLCPTGCLTCSQSNSQVECQTWIIVASFYIDNVFTTDGWNILNGDLTKQQCSMIPSICGYGVCGKNTKLILTLVSLPFHTRMKIKLKYLKIDGWDNNDYSILQINQNIKWKQSLSELDQYLYGVCGGSQKDIFVNVELTFPHLFTSTQIIISNTLDQNHNIESFGVRDIQIFVQQSICGDTIVQEYEECDDGNMDPFDGCFNCLYSCIDGCSLCHQGTCLGCYSGWQYLRYTSTCQRVFEVEFIKLIDIFIQPFCYIEGCLDCVNDICILCSNGYHLNLEYNICQSICGDNLITINEECDGLLNCNQCQFNCPNYCNQCEFGICLLCQQDYILQNNNQCIKYEMVSLCQSQCEICVDSVCYKCQYGQQLVLGQCLEICRNNILAMYQFDECICNPYCSDCRFGFCYQCAKPYFLINNTCISNKCGDLIIQEDEECDDGNEIEFDGCYNCQFSCSLDCQECQYGKCIDSCQYGYYFINNKCTTICGDQIIAGNEECEDNDGCYQCQYSCPINCNNCQSGQCQICNPGFILINNMCTSICGDGILSNLEQCDDGNQNDGDGCTFNCLIEINWICKLGRDCNYVKYPTIITEYLMQKNQYQYVNIAFSQPVLKQSNINYLENIKASIVDLNETLFNITIMEVQPALINQVSEVQYILQIEIFKNLDYYPILEIILTEQLYNNNLAPLIYMKEYLQLKQSNYINQEQIQMARTFQYINEISIKSLCLLSILPMLLGNALSFWFILDALQEQSYLKYINVLYPQTLIIYFQSSQVISVNEILDIISQYKNQGRLLKFPYLQAYEKFEFYEVNADIAEGFRAEIIVFATLYLVYISTLFFVKILSKLESSEFLLEWPKLVRYIQKMKRKVYKKVKETKMYALKNTLRACAWDLIFMSLLELNTDHDFTYSRTWICILIATLILVITIILLLNQVTGLKSWQKQNFNQFWSHKRDIFQVTKKFLILYILIFYQYEQELQTLILTLINVFYLIYTINFKYVDYDNLEYAKVIVMETSTTIFTASTFVNWNILSSYLLYTQKITISWLQMSVLLSVLIFFIFIELYNTYSIVKQKIITSRTKSNLKLQEIQNVDPNSKFQNSKIFQKNNKTIFQRAQYPRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9N1</accession>
    <name>A0A8S1Q9N1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1470107</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9N1"/>
    <dbReference type="OMA" id="FFANARD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008504">
        <property type="entry name" value="Emc6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029008">
        <property type="entry name" value="EMC6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20994">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20994:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07019">
        <property type="entry name" value="EMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8111421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="109" mass="12466" checksum="A96C928A63BA6917" modified="2022-10-12" version="1">MEEPKIELDDLEVNQERLKQNTNQLKNLRVYGSLNAGIIAGILGMDGWIGIAIYVVIFLMVSACLAIKMNFKVKEYFRSSYDAYYSGIGTDLLLFLMIWVIFHNLVNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCF8</accession>
    <name>A0A8S1QCF8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1550090</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KSAFECK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001155">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045162">
        <property type="entry name" value="Vps15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583:SF0">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="674"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="1641"/>
            <end position="1682"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="2055"/>
            <end position="2088"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8113499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2088" mass="246180" checksum="18B2CD76B74DF999" modified="2022-10-12" version="1">MSNRKTYSNKQTKISLGEIDEMFNDAEFSQAVKDTFKKQSSTVRHDGPIQTTNMFYQKPTISQIMRLRAKGSHTGTRCPSLKKEERKTRWASLISEGRTEHHKRIPSEIENIPICAQYEQYSYLCREHEQLIQNLSELPQSIKMMLYSNMYEVQRAKMCENKTILDDKFIFTLFNTLQQLDKQIIEPLHQQQTRPVLNFKMNFVQNAVNIISETIIQGVREALKHDTRLGIFMEGFCKFWIVLIDIQLQWMGKSCADYIEAQVKEFKEQFKEALAQKIKVEDQMIQMDKEFKSMEAQYIRKHEYLQQQLLSVQQEFQDYVVAVQQMSDLKIAEERMNSVGLKALELESMFKQYDKVVSQHSFVFQKDIKELGTALIQQKKQNQPLPMKHQEAQIRLGCQKLPEENYQLYQLNSIGIFMQVHPFQILFEINKELEMDYPMLLGNFLDYLSQIGEFQSHVCRIFIDWVGEEDKLNAILTYFSECDLDQPINQLYAIIFGIREHRQLSSVSLKKLIKYYKELQEQHDYQLNENFTIQEASPYFQLLFSQEFNEKITNSQKQQMTLLELLLMLAQHFLMIENKQWSGWLYKQEIESEDLDMNVIKQFIELRNQLNRPLEDSLKLVRQLSLLHPDLFYKSKQAPLERMKASVRKVQRKNSQRSPLKRSMIYQLYKKMGNTLIEDKGKINLMKAFETYNTEQEIGTRQTLFFENKLFKTLSYKWHQQTLLLKVHLMRKQLTLQELHIHTQNFQYLIEQLEPYPNLMPFHNLINFGSMPKPQPSEMSLECNKEYLAVSRQFLYLTLEERLQSNYKLPKCLLNFYALQFLSAVNTLHRRNLYHGHIRSSNVLLTSLDFLILADFASYKPTYLNELEIIRNIYKSSIQKCTLAPEKYSDLQEVQYLNNLTKAQLLSMQAMDMFSVGCVLAEIFTGESLFSLEQLLQFRKGEYEPIIKIDGIIGDLIRNLISINPEIRYNSEQALNLFCNEICSSQTYELLQYISIALNVNGYFMQPDARIALLRKVISTIERKQFIYTLPLPIQFINLSKFNQICRDFEFPRKMVQIQFTSGVGMDEIIANSLENSGNLIQREFKEVHQITQSVSLQVSMVQKLKQSIQKQPELQGSRQNLRTSTFTNIDIRQNNQEYIIVILWISNQIRNSRFMQTRLCGLELIEFLCQYIDDELIYYFLMPCLGILCEDSDSQVQYYAFSIMLNLLKNIKNPPQSSTDTLIFKLYIWEQIKNRMNDMSTNHQLALKIGDIAEILHKYNRLEEKILFLQIIQKKFQENRINELTIIISKIYEVLKYYECNQTLEILTLLISQFNNTTLKIALLDVYLEIYKYYLNTENSQLLLYCLQLNTRQESALFRIIRFVRQATEEQLLEFNKVIQYVKEISPLILHHNRWIREEAKEFIIILLNRYSSKQIFFKFHPHIKKYLKLDVPIINLEIFTSLIQKPICSKWFDQKNQNEPRYPTLTERELYNYNNYYEEYFKKSKNIQEIAIQQQIQKAQYPIAKMNQRVNDFIEAAFRVGLMQLDQNKWEDFTKKGGKTWEDFIQYIKNNNGFDLSNKQDNVLTKKKVKYLQELHLQLQYQIPQSSTQWINLFNYNIIANQIYIKSSQQQIQDIQFQRQIQQPITRPFKISNQIVTTISEHKGIVTSLQKFGDQRRFISGSYDGTVRIYDMKKIEEDFTNGSVAQIRISEGDVQSRISALCALEGTDSFVVGTNTGSVSLYTNDRHTSTFLKGDSEIRKIVDQENTFMYVTEKGTLKIEDIRSRNGQSFNIGRQRGLVSSMVHDNYTTLIGTINGYLILYDIRSTMIVNLFQLLNHDDQALPIFSLAYMNRVSQFEGMNNDSQKLVGIGYQSNNNEVGFWDLNKQNEDLNPALFLYCSDKKHPIIETPRLQSVLKSEQFINHNNNLSTQCHYDFLNKSYQVDLISYLKDPLTSSERVAKDRWLRGTKNLYINLKSAFECKSTVTSLICLTSRIDNQRQENTFITASTDRSIQSWTLNSDVSKQQDKNNDREFQTNIISSPYNSIRKFKQCPSGDVYVIQDYEFKNQSNSGNSFEHKDCITDMIYLDHDNSYLVTASRDHTIKIWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFZ7</accession>
    <name>A0A8S1QFZ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1580043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000163">
        <property type="protein sequence ID" value="CAD8114134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFZ7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="174" mass="20552" checksum="94870AB49552FECA" modified="2022-10-12" version="1">MVRSLIYSSQILLICSKQILVMKDTQSSRITPQNLKAVQLYQFFLKNCLNQLKKHNDIKLYILLSCYLINKCFRTFEQTSLVFYIFQHQLRANDFQFQVLYFLSKQQQSQDSVKELLKQDFYIPALILSIVSGNNSYKLCYILIQQQNQMKLKILVSFSILSKAKARFIIEKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNX3</accession>
    <name>A0A8S1QNX3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1710059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000180">
        <property type="protein sequence ID" value="CAD8116395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNX3"/>
    <dbReference type="OMA" id="NFKDSFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13179">
        <property type="entry name" value="RanBD_RanBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045256">
        <property type="entry name" value="RanBP1_RanBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF87">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE RANBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="1">
        <location>
            <begin position="30"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="168" mass="19586" checksum="550F058D2185CCC8" modified="2022-10-12" version="1">MDPHGDDDEQVGEDYNPEQEVFVGEGQATLPQLPVQTGEENDEEVAKFRAKIYRFADSQWKERGVGDMKFLKNKQTNKIRLLMRQDKTGKLIANHFITAQEGFCKLSQLKTADKSWIWTCYDASDEQPKVWQLCVRFTSPEEVERFKTEFEKAYESNKALQEEVPKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPE8</accession>
    <name>A0A8S1QPE8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1900002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000199">
        <property type="protein sequence ID" value="CAD8117151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPE8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="8473" checksum="8C8E4E641D4B0A77" modified="2022-10-12" version="1">MLKKKIMKIFNNNFKNYINYHSHFLNLATNINKIFNQIYFQLLIYSSQIFLLQAFYKLLAIIILIIISEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQQ8</accession>
    <name>A0A8S1QQQ8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1900004</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000199">
        <property type="protein sequence ID" value="CAD8117155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQQ8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8117155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="36825" checksum="A6A7A0CBF6970C98" modified="2022-10-12" version="1">MMEMMSLMMDVINANFNENYYVKSAYKADVPSVLTETTFKIKFVQVHVEMEQFLHLNNAMMVTMLQEMVVPNALLIQQTLDGLTQQSNNCKNIFGDGIIVSAPILIFLNIVMMIISHMIMMVAHRLVSLNAKNHQYLKLVYPIYVFNCVDVYLLKSRLHRCECRESCLKYDLSEGNGCLKCKIGYELRDKQYFTICGDQTVTPDEQCDDGNLIFDDGCHLCQYNCQDTCALCFKGICLKYFSNYLLILKRCIKINYEDNIRSQIDFNFPYQSYDNQEWQSINFVNTIIIQKPSLDNNQDYKQKRLCNKWNKQIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QRW5</accession>
    <name>A0A8S1QRW5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2140003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000223">
        <property type="protein sequence ID" value="CAD8117872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QRW5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="383" mass="45304" checksum="752C1FD32A4B290E" modified="2022-10-12" version="1">MFCLLLQYKNLFKLLLPGLFQGCIIAEIFVEMDQLQQIQMDSILNNMMMEIYFIFNDLCEFQYEEIKQYHRMNNLRIHQHCPLRVVRIAKQNVNPFALFAIIVDQVAKLVRMVTKKIDNLFYSISGDNILTEGKKCDDGNLIFCDDGNLIFYDGCHQLSFSRPVACSNCCLDFCYDFMKVFICLSIPALNFIISIPIRNEILNARAVVQEEMLVDFQILMVLALLYAEIKQLLKTNNVMTVMSCLRVDFIHVYNLSLVSFVFIRINVLIQSKRLIQRKLIIFIFQIQSSLQSQIVEIDQFIKQVLIKLVNIQKLQINIFQTLLEQESQGNLVHLIMRNYQYASDWKTLLYQIIYFTKFQGYLKKNSPFRMTYFNKYQQYKFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP20</accession>
    <name>A0A8S1JP20_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ZZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0050038</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JP20"/>
    <dbReference type="OMA" id="IHKANIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052260">
        <property type="entry name" value="Autophagy_Rcpt_SigReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15090">
        <property type="entry name" value="SEQUESTOSOME 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="6">
        <location>
            <begin position="185"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="57"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8043387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="242" mass="29290" checksum="1DA20D559809E0F7" modified="2022-10-12" version="1">MCYCLNIKDRVIYLACPIDYNLFIERLQYLKNINFPILQIIQLRQEQFVSKEMIKDVEEWQERLSEGYSQINSQQQEIQQLDEQFQHLSISQIHINEQVDYHKCQFCYQNLENSKYFRCIVCYYFQICDMCNTHKREKDHSISHIFIQCNHVTDWNKLKQDSKMKIMKQKSNMMKRFKIHKANIHELICCDFCNSSPVQGIRYQCCECPDFDLCKACIKKFKHDPTHNFIQLTSNIEFLIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRT5</accession>
    <name>A0A8S1JRT5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0080321</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRT5"/>
    <dbReference type="OMA" id="FNDFQMR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="102"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="243" mass="28611" checksum="04DC2E7916C0CABD" modified="2022-10-12" version="1">MKQSEQVLLASQQPGLIDTSTKRLISKSSTQFQTPSFQQPVVTRRIHRKLIPIDGEAEETVVQPIQPPPQPIVHPVQQMIWPVAVQFNEDDALAKIREEKMLALLERQSEMLTNLLQKYKRLEEEQTNKIQRRIKELERENEEIWQRRRQNYLIYNVSQSVDQKLKEFNEFQMRPFLDQGQPVLLKPIHQDDRLFFDKKKKRPKPKKIVEVDDGLVQDPDTGELWDPKTGEVVQQPPPLQQPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JT81</accession>
    <name>A0A8S1JT81_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0080388</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="5">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JT81"/>
    <dbReference type="OMA" id="MTKIGKG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07852">
        <property type="entry name" value="STKc_MAPK15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000238">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001058">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="13"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8044819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="494" mass="56787" checksum="15C6BCD0C29E2E6A" modified="2022-10-12" version="1">MSEKIDSNILKKYEIMTKIGKGAYGIVWKARDIKSKRIVALKKVFDAFSNPTDAQRTYREVSFLKQLNHPNIIQLIDTYPAENQNDLYLIFEYMETDLHIAIRAKILQPLHRRYIIYQLFKALKYIHSSGMIHRDLKPANILLDSECKIKLADFGLARMVCTLEQDILTDYVATRWFRAPEILLGSKSYSFGVDMWSIGCMIGEMILGKALFSGTSTINQLEKIIEVLGIPTPEEIQSLGGQNQLFDKYPRNYKSNLKTILNSDEDELDLIKKLLVYDPNKRISVQDALKHPYLKEFYNPKEEITFKGQLILKLQDDKQYPISSYRDLLYKQNLDLTKILTLVNKQKSLIQNIQSNRSDALKKQSSQQQFLNQQFAKSAFMKSNSTVNIEKSVNKTATATLHNSNSKQPQNSNNSSSTQIKNKLKQICLQSADKCEVVVQKKSPNSKISQQPKLLIQSNSKQQILNNVTNTSISPSQSQQRLQIVRQQHQQLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JW89</accession>
    <name>A0A8S1JW89_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P-type Ca(2+) transporter</fullName>
            <ecNumber evidence="2">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPRIM_AZ9-3.1.T0080252</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000191">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="769"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="841"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="889"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="926"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="957"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="18">
        <location>
            <begin position="312"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="18">
        <location>
            <begin position="794"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1019"/>
            <end position="1044"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8044686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1044" mass="116455" checksum="231CC0F39FBA3029" modified="2022-10-12" version="1">MTIILDPKGDRTQFKIYTKGAPDMLLDKCSHYINAEGRAVVITNDYKQKINSVIKNYASQSLRSILLLYRDTMIQGRPSKPEEFNNVEDIIDKSYTIIGVTGLQDPLKEGIIKAVQQCKEAGVTVRMVTGDNFDTAVAISKKAGILPPNYEHHEDSLAVMEGKTFRQMVEGLEYEKDDEGNEIPKVKNLSNFTTIAQELKVLARSSPEDKFLLVTGLKQLDNVVAVTGDGTNDAPALKKADVGFAMGIQGTEVAKEAAGIILLDDNFASIVTAMKWGRNIFDCIRKFLVFQVTVNVVAVSMAFLGGVFLKESPLTSIQMLWVNLIMDTLASLALATEPPTDELLTRKPYGRKEHMITPGMWRSIICQAAFQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLKEELLCIYNFLNFSITNDYKQKINSVIKNYASQSLRSILLLYRDTMIQGRPSKPEEFNNVEDIIDKSYTIIGVTGLQDPLKEGIIKAVQQCKEAGVTVRMVTGDNFDTAVAISKKAGILPPNYEHHEDSLAVMEGKTFRQMVEGLEYEKDDEGNEIPKVKNLSNFTTIAQELKVLARSSPEDKFLLVTGLKQLDNVVAVTGDGTNDAPALKKADVGFAMGIQGTEVAKEAAGIILLDDNFASIVTAMKWGRNIFDCIRKFLVFQVTVNVVAVSMAFLGGVFLKESPLTSIQMLWVNLIMDTLASLALATEPPTDELLTRKPYGRKEHMITPGMWRSIICQAAFQLFVLLIILFKGDSIFGIESSRGHRLDEEYNPVYQEHYTIFFHIFVFLQVFNEINARKLKKTELNVFDGFFNNYLFISVIVGTIVVQILIVQFGGKAVKVTPLDFAHHVVCIIIGMCSLAVGYFIKQIPDQYFQTIELFKEQVAPEADPDTLQGKFKRPSTFLRKKRAIENKQTNKGSQEIEMKAGNSGFKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZ83</accession>
    <name>A0A8S1JZ83_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZ83"/>
    <dbReference type="OMA" id="KQSHQAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="224" mass="26568" checksum="7817AA3284390D84" modified="2022-10-12" version="1">MNFLDFIQQQIQENQDKPEEIPLQINEEEPINLNENLDISANGSSSDEEQLYEEYFKEKPSIKATYDEIYEKLKQNSPPQTELENEQLQIIAELQAKLVVQETELLILKKLHFDLKNKQSHQANKIEQLQKEKDVMTQMKIGYDKKFQKLDEKRKIIEQNQRIIKKLAMSTSNDYLESAIKRVNQQVKINDKVQILQQQPIKENPQVRKLSNQKQQNSFRNNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2F7</accession>
    <name>A0A8S1K2F7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T0130044</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2F7"/>
    <dbReference type="OMA" id="HETMTAD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000277">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF97">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE YME1L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="180"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="520"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8048709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="563" mass="64262" checksum="57EE83BA52714D84" modified="2022-10-12" version="1">MKKFNFLKYQFSTLFEYQNLSQRNKLNEYKQGFLISKDFESYYNYIKILSRLKMYDEILQAKSSVPIFKQIQFWREPKNKVIKDWIRQAQDEYYNQNPQDLPLPWFVKYPIIFFISYEVFSTIFDVDIEISLNIIPEEKEVKKTTGFKDVIGIDDYKEELQEVVDFLRNPSKYHESGAKLPKGILLVGRPGTGKTLLARALAEEAGCTFLYKSGAEFDEMFVGVGSSRVRQLFQEARQKQPCIIFIDEIDSIGGSRGGDDPKRYGTINQILTEMDGFRQNEAIIVIGATNMEQSLDAALTRAGRFDKTIHIMLPDIKGRKQLFEYYLKQIKHADIDTQLLARQTTGLTGADIENIVNIAIMNAVKEKRQKALANDFQHAIDRTRMGIRYKLTDQDKILTAYHESGHALINLLTENTVPLDKVTILPRGSALGYTSMVPKEDTLSQTRGNILAAIDVCMGGRAAEDIKFGKDNISSGCGSDLANATAMAYAYVKDLGMGDTLISDAQTSKKFSYLVDLQVKQLLEESYNRVRNKLEQNQQVLSRFAEELLKHETMTADQLKRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4A3</accession>
    <name>A0A8S1K4A3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Myb-like DNA-binding domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0110267</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4A3"/>
    <dbReference type="OMA" id="QMIDSCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000010">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000016">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="119"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="121"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="169"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="169"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="221"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="225"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8047116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="388" mass="45970" checksum="759EEBB655400C04" modified="2022-10-12" version="1">MRSAHQLITQFELNGFCLPQMEKNMCITFLKDLLQQSPIDMDFILANTSPSQSQRRLLTTKSNILHLLNEDQDPHIIKPIFDYSQLENLIPKKNRNKINEIKTKVETLLNSNNIDPPLTIKKRWWTEEEDQQLKELVTQHGAKNWKKIASYFEERTDVQCLHRWQKVLNPDLVKGPWTQEEDELLIKLVTNCGPKNWSQIAKHLPGRIGKQCRERFHNHLDPKINKERWTDEEDQTIIEAHKKLGNRWSLIAGLLKGRTDNSIKNHWNSTLKRRLKMQNRWEDLQVIPRQDETLVKGVPRRQVQRRIMYYKTPEKLIKRDPVSRQLNFQTPYSNTTPKSEQTPKNLSIVYPNLLVKDAQMIDSCQVLFKQLSELTNMDLDFNKQYSYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K5F5</accession>
    <name>A0A8S1K5F5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0130211</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K5F5"/>
    <dbReference type="OMA" id="VIICKFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="526"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8049065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="64980" checksum="FBBA8DF4310D2D81" modified="2022-10-12" version="1">MFSIQIGLITALFFKISIQTNRIRSHPNQTYMVNEESLEFQFSTQEAHVKLLSNPAIYDIIVYRNDSNSLGTYIETPFLSLDNQYLDDQQLDNDCIGLPKHPKLTEIFSVNLLDNTEDIYYTDILILEQQQKIFLLTDELELKQAVLQFNQTQSPQWNILIKNISYHLQTLISQQINYTNAFFACFQTDQDRCLILSNYGGFWLNTNSEIENPNLNLEPVIIQNEDLNKVSICQEYLAIANGNAGVDLYQYINKQLIYLLTITAQQLNQTIVNIISVKLSSQRISILDEHTGLYIFQLQNGQLSLDLTIPQSRCVAFDHYENTYLVVAETPNNIEYMMEIFLLSDTKEYYVNRIYVDDFSFRDIEIDEDYAIIIGEDVHLVLRHSIFNGFMRNNTDLVKTFFEDELIRFQYFEMNESIKTQFMETSYYIGLSKGSLHIWKFNDYNPVIICKFQYGREQNYTLKANTSKCEGHQSTDLFEQCQITQQLSIQASGPLLESDSNYLIIGVCVSIAIILTIIVFILCRKGRALAKRIQELKQQAEEMKKYGAFQEQEQQSAEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K603</accession>
    <name>A0A8S1K603_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0140182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K603"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8049823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="99" mass="11849" checksum="FC9C3FB707BA63DE" modified="2022-10-12" version="1">MNYQNNIAGLQNKVKQTRIELKIQSILKNAQDNKNNKIRMDLNGIPISKNSKNYTVNFKQNLCNVYIVENWKKYNILENNNQLYEVEYKRKCMKNCNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8T7</accession>
    <name>A0A8S1K8T7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0180268</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8T7"/>
    <dbReference type="OMA" id="KQPNAYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="58"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8051659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="386" mass="45150" checksum="BF6AF39F903B87EC" modified="2022-10-12" version="1">MKKKRQIPQDDLQNNKKQPNAYEDLENTQSNVTELIKRMKFREIEEFEIQQFIASQNEQKFLMITGQPGCGKTMLLTKCLKQWQNNYTTIYINAMQCKNYTEFLNICKKQLNVKSTSNKQQARKIIMDTLKESNTIITIDEFDNLFKVSEKEAFDLFSLSKYAIIIGISNDIEFLQTQSVRYKFQLPQFKNLILKPYTIQQLQELVLDILKQIEIQKYDENAIKILTTRAYNDKGGDMRNIIDIVKRTLRDKDEITTDAVNKEIGMTVFDNKMKSVVTTLSLHQQLILLGITALLKKDTSQIEIDLSDLIRKVNEIKYKLCLPLSVDVEEEINLLKDYNLLTTKEVKQQKMGFKITVKKIQCKFTAEELKYQLSDLDAFKNILQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K962</accession>
    <name>A0A8S1K962_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8051103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K962"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="133"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="371" mass="43957" checksum="B3A7D81C6E5BF0C0" modified="2022-10-12" version="1">MMKSEFQSQFIPLTYQSQPQYTYQQKDFCNAIAINHNNTILVVAADKHLNFQFQRSQIKVLEIIYSNTSTIINLNQIQIIQDQHFGFINTLNFFKKNSFMLDNFISGSQDNQIIIWQLEIVESSKFWIPIFKLKEHSSSINCLVLRPFSEDLIISACDKKIKFWSLSNYQFLSPLIYQGWECFQTIKEHKSYVFGLSINQEGNRVVSCGYDKQILIIEESNRRKWYMKQKIQTYNMGFRICFINNNLFAYQSLGINLQLYQFNEKNQKFLKIKEIPVNGGNCYCSPYFPSSYINTQNLFVTKNGYHLNFIRFIYSHNQSQDFQCSLESSIEFDQKGYWGYIYGTISEDSKLLITWDFISEQIQFRTQIIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAC3</accession>
    <name>A0A8S1KAC3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0190008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAC3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035712859" evidence="1">
        <location>
            <begin position="19"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="180" mass="20936" checksum="FBC7C3A87F4C2EA6" modified="2022-10-12" version="1">MLKSCVILVLFAISLNYCQRNFRYCQRNNKIKALTRGYYKNVISYDASPLLTSRKQNQFAGVGNEMGILFRNEIYQASSNQRGTYISLGQSIIIELLQVYELNTLKIVLRNGNIRTYDFIVYASDSNEEIIIFDSITNYIEVVTIKFRNQLVKYFRIYNRGGNSEAPFTHIIKVEACFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB92</accession>
    <name>A0A8S1KB92_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">V-ATPase proteolipid subunit C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0170296</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB92"/>
    <dbReference type="OMA" id="YSLAKGY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000001">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="10">
        <location>
            <begin position="25"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="10">
        <location>
            <begin position="103"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8050915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="165" mass="17047" checksum="4C220F435569C783" modified="2022-10-12" version="1">MLFILDTMVSSDDTPISASSFFGFMGVTMALVLANLGAGYGTFKAGAGIAAIGIWKPEIIMKSLIPVVMAGILGIYGMIVAVLLSQKVKNPLGYSYKSGFAHMASGLCCGCSCIAAGFAIGIVGDVGVRGNAQQERLFVGLILILIFAEALALYGLIVSLILSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCT4</accession>
    <name>A0A8S1KCT4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0180115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCT4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8051475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="63" mass="7485" checksum="E8C6189E62A903FA" modified="2022-10-12" version="1">MKVQLRIANQQVNKQFRFSSLITSLQYFTFNKTNTIGVIELLEINKILMQLEVENWIRKGNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEI9</accession>
    <name>A0A8S1KEI9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0190309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEI9"/>
    <dbReference type="OMA" id="QRRNDWR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="148" mass="17886" checksum="007D0FA5DC0E8E76" modified="2022-10-12" version="1">MSEVEEINLEQNIKKPRHQRQQNQSRRIRKTKNNFNRPRNNSQNRNRDRQRRNDWRQQNGANSRDLYEIDIYNFLAKNQISDLMSDIRSRCGNLVKLHIIANNIQENEAETKVVLKAFFKDQKSAEECEFQYNDAKLDDLSLRTERKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KFA5</accession>
    <name>A0A8S1KFA5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KFA5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="98" mass="12299" checksum="87C8FF712698D38E" modified="2022-10-12" version="1">MKRYIVYMYQYKVRLKFIRQILQEKSQKRSQKEFKFIKQLQKRQEEEIEKGEERQERKDFIKQFKLCEFRYCGIIKNLKIKIQIQVKIKVSFKIKTKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KFA7</accession>
    <name>A0A8S1KFA7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phage protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KFA7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="63" mass="7683" checksum="424F7558518619E4" modified="2022-10-12" version="1">MLQRQKHELKEQFQDRKKIYELVCKKNADRAIVMSNIYVNIKYMGNKYSKELEDELNQYIAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIY4</accession>
    <name>A0A8S1KIY4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0220152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KIY4"/>
    <dbReference type="OMA" id="ICHYDCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="706" mass="83307" checksum="91EDE9628FF3BAD8" modified="2022-10-12" version="1">MNQESSVILAQLPSQMQNQISQLINIVSANIDRRRFDQKREIQLLSLIVKLFSGGISSIEIFLRYSPRDLLDKGFTLREAYILIRELGGQCKCDFPKPSNKEIEELCYDKDIRQLVLYKIPFIQPIANQKYKYILILGPQKTGKTSFLFNLINIYYDVDVDSLYRVKKQIKNRDSLFYNEYLVEMSKQENLIFVDFIGMGDLEKDIFDDNKNNFFQVLLEYVQDKEIVAAFFVNSASINRLGQEEIYTIQRFFELFHKSFSQRIFLILTFCTDNNPLLSVYSHHNSPTPTILKNKINEYQNQIEQKLIPNEDLKFWFGINNNYLYFPAIDLDHESEQILINHNNINYQDFKQNCQSVEVGIDLTQSYIESENLILQENEIEVTPRQILQFYYEIMRKELLKIQLKINTIFNSQNLEKLNQINHFKRLLKIEQEIHSLGILPQIHKSKQPNKNLILSTNYITFENKYEKKQLKSTKGKKILFCTDCQKICHYDCELDSKSDLAEAKKMCQNILINFKFLSQMKNPTRVHKESSLDILYYCKICSQRDMINQSNNSKGGQTCLISKHILINEVVFDVNNDIYYISNEQVQKTFKQDYDNLKKQKQLYVMNQTLNLNRKIEFLKKIKSIKHQIKKINDTQDGLVSLNLQVRKDLLEKTQRAGIQIKSSNFEGIQNVYNNLSYSQNQQIIKGNNKILFQLYKLVQKEIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRX4</accession>
    <name>A0A8S1KRX4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0250290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRX4"/>
    <dbReference type="OMA" id="WYGETPK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000184">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="89"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8057151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="263" mass="30600" checksum="93F5330DB97BE008" modified="2022-10-12" version="1">MRIAQHSKSCQILLQKKIFTFDDQELINTAESTPDSSGNSEKPQLVVFRSQILPDSQIARELHEASIQSYWYGETPKEYLDKRIVKLKKQKFEKVVVLDLDETLIYHKNNKTQVRPFCQEFLERLSRICTLVLFTAAKQDHAINMLKIIDPNKKYFKAICTSEHMIGNVKDLRIFQTDLKDIVIVENSPKKFIAQINNGIPILPYEGQQNDNQLELLLSFLEIVLLVDDVRIQLANIFKLQNFYKELNGKQAINKLYNSKKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSS0</accession>
    <name>A0A8S1KSS0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0210254</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSS0"/>
    <dbReference type="OMA" id="KNPNIRP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000726">
        <property type="entry name" value="Calcium-dependent protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000634">
        <property type="entry name" value="Calcium-dependent protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000382">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="34"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="352"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="442"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="478"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8054014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="533" mass="62126" checksum="25184213AC24E9C3" modified="2022-10-12" version="1">MGCLQPKKLNRIVAMQTTNKDLVFTSTADIHKLYSFGKVLGIGAFGKVLTAKMRNNNSKHYAIKMIDKRKVRGREAMLANEIYVLQKLDHPNIIKFYEVYQNELYFYIIMDYCEGGELVERIQKSQKNLSEGQVQNIIFKICSAIMYIHEQGIIHRDIKPENILFSTKDPNAEPKLIDFGLAIKFDSSNLKQLKAAVGTPLYLAPEVIEGKYNEKCDVWSLGILLFHLLCGYPPFYGKNRTDLYENIQYQNLIFDRRHWNNVSDEAKDLIKKMLNKNPNIRPSAKDCLRHSWFRRKFQNPVKLQRGRSTIISMKSPQSEDQRSIYQMLKTFRIGAKFKKEVMKVLVNQMNEKELGRLKQVFQKIDVDNSGTITVQELKEALMEEGSHISHEEIEQLIQLVGFEILEEDKDDNDCASTTKSTKPLVIKYSDFLAACIDERRVLTREKLWSVFKYFDTQDANFIMKEDIKEALARHGRQLSDEKIDEMIYEIDPNHDNKISFEEFCQMMGVAGVEQTMDFKDEIKEHQIFQQSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUN1</accession>
    <name>A0A8S1KUN1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine protease</fullName>
            <ecNumber evidence="4">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0280236</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUN1"/>
    <dbReference type="OMA" id="SFHRMDP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF49">
        <property type="entry name" value="CYSTEINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="5">
        <location>
            <begin position="55"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8059109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="402" mass="47846" checksum="893916FF10BE288E" modified="2022-10-12" version="1">MYNLCGSLLDRWYNVKFNLTQYVYDIDKQLRQKDKVIYILNETIKNDVGIEQRIEKLKKTCSQILWFSYRKKIPQFQISSFTSDTGWGCMIRVAQMALAQVIRHYHSFTQPEQLIVLIRHFIDDDDDEMIDFIKQTKQNQVEYYHAPFSIQKIVYHAKIEFKKEPGDWYKPNEILEILNYLFKYSQYSVNMQIYINYECAFILQDVIQQMFNYDKGNLEWLKECVKNNIQQNQQDDKGIGIFLPARIGLQNVNKDYLKVLNHLMTLPYFQGIIGGVSNKAFYIVGRIQDYLIYLDPHFVQNAQNFEDLSKNQASYSCQNIQLIHNQIIDPSIVVCLCIRNGLELLDLWNSVFIIIIYQQLNHLKQEFQELFFISLLETNTELQICESFQYLDENHELVNILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWW7</accession>
    <name>A0A8S1KWW7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0270184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWW7"/>
    <dbReference type="OMA" id="IVFTHQV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="606"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="628" mass="74587" checksum="A828BD385C807996" modified="2022-10-12" version="1">MVSDFKKKVDAFLKKNNQKSNLMSNTKIASSLKELELLQQIKNEELLNKSLCSNKYTVKTEKKSMKAMEIFILGNNKVVTKQVISSELIQSRLLKQMETFLKDDRFNYDLKMTTMIEQYAEIVNRIHEKAFTIKIPEKYIIQMSDYNEYYQIKEDTIENFLENKLEIIADQIIQEIQQSKRTTPKILSHKTTEIYEPIEQPTTIQQIEDLIKTEPNIVHQQEIIKQESPKIEQIIPDRKVSNKKSRQRKKNQQTKNEKNLSEQSSLSEIMDSSLKNSNIKDNDDIRRRRKEKSKITDQAQIEEEEQDLIQKEIEKQKKQKIIKKLREFNQLFKPQMYSRRQSEDTMLQIEEPILLAQPKQKILKTLSLRPADDTIIMKEVYEIIRVKKQQLYEYQSEQRRKQEQALENSIQNQNKIQQKPLNLIVNGKHISELKEAAELWDSKRNSPRSYTKQRQNQNNSTVIEQKQEEINVNEQMNNMIEEQNNQTNSQNLQDNNPDNSINYQQIINEKLINLEEKLKQQCQDIFDILLASEFVYDVKIDDYKIGKPHSNKINHPNFIFQQEQDEIVFTHQVLWRREIQQVIPKLNSVNKQMSVLINSEDEYVQDSQQIISEEEDEDKSDQNDDSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXR1</accession>
    <name>A0A8S1KXR1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0300050</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXR1"/>
    <dbReference type="OMA" id="MAPEITM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001020">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001178">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="575"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="475"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8060269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="826" mass="95832" checksum="9D373F2932BA344C" modified="2022-10-12" version="1">MTDVLPERLRDKLNILQDNLKRALSGYENTDKKLPTRSYSNFQSSKIGIRIWDLDDKRHCNNLMISLKQLEKQERTYFFEKVKPKQRIRIDCIFKPTQQITPLSSDPSSNPSNLKISKRLSFSDSLVLSSPQKKANELFIKLNLQTINKSSPIKIDESIKANRQLPNQEQGFNFQQIFSNTSLQKSVSMSVKSINEYEICSLKEILQKKNGHKYHSMEKDQINQNSVLLFKELSILNDNANLQKQVLLKNNNNIFQFQSMKQDQIDQVAKEIQLNFSNLKQEQIIQCSVIQQLPKFDLDSTKYNHSLKILERQTKIDKISRLGIIISTIKKETQQQNNNQIQTSLIQIKCQQLARDVGQRKPNILNFYVNTLDELVVRQLPQENQPATIMTAFKIIKMDQEPKQNTINKLINQNKQLNYEQVFFNNEPQILSLKSINLSLLQQDKNNENNILIMDLMNMVDKECFENTKIQPKICFQYEQNLEKNRKKIEEIKQLISTSKKQNPLTQLTHSKQQSIKEQKLQIDILKPVRNISVCSNERRQSSVGLNTPIRSPNFNHQKGFMQMSKNDKSALNRFKKIKYLGRGNISDVYSVIDTTTGMLTALKIIQKSVITSKGIQGLIKTEICIQSFIQHPNILKCYGVINDDKQIALVLELGDITLFNYKKEKKLTEKQIIDIIYQVLKGVNYLHQYGIIHRDIKPENILLQGEVIKLADLGICIKANNAQQYCGTPGYMAPEITMKQKYDNKVDCYSIGVLIHELLFGKIPKSGQQVNGDTQLIKLMNRLLEINPALRISCKQALEYEMFRFCEKKMAQYSEMIKNTIFWDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2A2</accession>
    <name>A0A8S1L2A2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2A2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="54" mass="6705" checksum="1A00A64C7718AEFE" modified="2022-10-12" version="1">MMCLIYKNPLEFAKRTQEIDRLICRFEKPKTEKKREINKKSNNWNMNMNKNKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L512</accession>
    <name>A0A8S1L512_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-tyrosine ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310242</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L512"/>
    <dbReference type="OMA" id="INLDSAH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="50354" checksum="41443A97091CB38A" modified="2022-10-12" version="1">MKSQQFSSTQDLRATFTTLKRQTFFYVGEGNNGELVRQILSKRPNFQEQRTPFNAHLIWKQSNKYFNYFQLGQLKSKKVTINHLEHHYVITNKNNLFHSLENYCNKTKMNMDDLVPKTFIINLDSAHVQTDLIKFLEYFTQCSKQSLNIWLMKPPDLNRGNGIKLFNDLDQFVELMENLNKKAQTSVKQQKSITLTLDNKRKIKLSIDDQDKQIVLQKYLETPLLYNQRKFDIRVWVLIDHKLNYYFFREGYFRLATERFDINNIRNLYIHLTNNAIQKHHPYYGKYEIGNQISFNDMQRYLKFQKPQLNTIIQEMKRIVKLTIDSGYHKLNECRKNFQFEILGYDFMIDKNGHVWLIEVNTNPCIEESSPLLKQLIPRMLNDAFKLTIDQIYETEQQTQTYPVNGYKCDENLWDKLGIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5Q2</accession>
    <name>A0A8S1L5Q2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0260318</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038800">
        <property type="entry name" value="CCDC17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33820">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33820:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="73"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="293"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="492"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="558"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="633"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="759"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="961"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8057984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1865" mass="220319" checksum="0D8EA108BB22D014" modified="2022-10-12" version="1">MNENICRDCKMTFESSEQLANHVKRFCINSDYGNLNKLEQKHEYVTKPAYPEMKPSLKQQDPSSLKNYGGYTLDQIKSNLKTNDDDFKRLENAVLRKKEDELIDQIDKLKQDRQQMRVVSDQDEHIYSILLQEVELKKEREFQCRIEKEQIRKLINEIDSWKMSVLEEQKKQMLEKLLVEREDLKNKQLQLIEDIRLFTRGHKTFFSMEQAQRVTDNRIIASAQYSEAKQQLVKLHTRLEYMDAERNRIQDNVERIKLGDFGEVRRGESSKNSIRNSQQILNDQSQIRNHQNYEEIARMKEKIAQDAQRLEQLKREQMDRVDRLKTSRPQSHLSNQVIDELQQLKNDYQQAGGQDPKFLNDVNNLESFYKGAVQQPQQLQQSQQQFQQQQQQQPNQFLQQQPIQIFQQQPMFINPQQQEIQTIKPKKKDFNDPFQQFSRKKEFFLQNNVRGVDLTREEQILMNLQAQEVDSLRMISRIPIGTEIYRFKMEQYKELSTMRAEMEKIIQEQRLQRVRRNFEKKRREKDKEFENNKWKIDQXDQIKSNLKTNDDDFKRLENAVLRKKEDELIDQIDKLKQDRQQMRVVSDQDEHIYSILLQEVELKKEREFQCRIEKEQIRKLINEIDSWKMSVLEEQKKQMLEKLLVEREDLKNKQLQLIEDIRLFTRGHKTFFSMEQAQRVTDNRIIASAQYSEAKQQLVKLHTRLEYIDAERNRIQDNVERIKLGDFGEVRRGESSKNSIRNSQQILNDQSQIRNHQNYEEIARMKEKIAQDAQRLEQLKREQMDRVDRLKTSRPQSHLSNQVIDELQQLKNDYQQAGGQDPKFLNDVNNLESFYKGAVQQPQQLQQSQQQQQFQQQQQQQQPNQFLQQQPIQIFQQQPMFINPQQQEIQTIKPKKKDFNDPFQQFSRKKEFFLQNNVRGVDLTREEQILMNLQAQEVDSLRMISRIPIGTEIYRFKMEQYKELSTMRAEMEKIIQEQRLQRVRRNFEKKRREKDKEFENNKWVDDQRKFIIENRLRNDFGEARKESHLYDPAEGLVIHWDYVLGLPRKTKLAQFVFAVFNNMQQLNQPKLIEPYDCEIEGESSNRVLIGETSQLYEIPAHPEVLIIVEVQLPVSKKLEENIGKTQSYGWTQIDLFDQQRQLKRGKFKCPLYKGPTDATITIENIAKLEPIPNAWFHMRITYPNDEELGQVRSIYPEQTAMEYNIPYIHLRSLFDRNYEAAIEGKEQNNKDNGKFSLNGQNYKVTEEVEVFGNKRKNTPQIKQYVLKGDMKKGYRVQIHFIQNKIANNFMRVMCAALTDQHLMMDPSGQPLAFNTTIHDPSSPNPGQNTESNPFPLIEADPQMKGISNKAARSILIEFNEEYRFFYNFQEQAMSSGKTIYLGFQVVEKNASQGGANTTMTMGEYEVIVLELGEILQIYXYDPAEGLVIHWDYVLGLPRKTKLAQFVFAVFNNMQQLNQPKLIEPYDCEIEGESSNRVLIGETSQLYEIPAHPEVLIIVEVQLPVSKKLEENIGKTQSYGWTQIDLFDQQRQLKRGKFKCPLYKGPTDATITIENIAKLEPIPNAWFHMRITYPNDEELGQVRSIYPEQTAMEYNIPYIHLRSLFDRNYEAAIEGKEQNNKDNGKFSLNGQNYKVTEEVEVFGNKRKTTPQIKQYVLKGDMKKGYRVQIHFIQNKIANNFMRVMCAALTDQHLMMDPSGQPLAFNTTIHDPSSPNPGQNTESNPFPLIEADPQMKGISNKAARSILIEFNEEYRFFYNFQEQAMSSGKTIYLGFQVVEKNASQGGANTTMTMGEYEVIGWSYIIFVKPDGSVRTGRNTLNLFNPPLKRPPLDESNLSQSGIKLDYSLYDFEYNDEDMIKWKNQRVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6C6</accession>
    <name>A0A8S1L6C6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0320158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6C6"/>
    <dbReference type="OMA" id="VEPMITT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="334" mass="39167" checksum="DADE0F585CE9137A" modified="2022-10-12" version="1">MNKQDKPESRAQRNIRIVMANLKEDLKYKVVKYFGHQKEEQELAKPRYAHSQFPKQQFFSQEAESNLIDFCLDNQGLKVSKVGTLVEPMITTEEIPQRPSIRKMKAIKQPVFFTEVTTTHFQPSERPKEVPASIKRSTKQQMMNKEILRRLGLLSVQAKRELEIEKLCNRALKVARIKDSPELNKRQIEVIQKQMRDAEKQSQELEIQRCTTSASAASRKAFDYQDDYLLECLSERPQLVYSVQSFRNRPQTQQAKSRIHNFFKTVTLAHLDNKDEIKKDDLSMQFQMQSIKESLQLCKKKLLDYNDEIEIALTEDIKGQVIEYKNIIKNNNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6R3</accession>
    <name>A0A8S1L6R3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tubulin--tyrosine ligase-like protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0310206</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin--tyrosine ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6R3"/>
    <dbReference type="OMA" id="NDITMHL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF39">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="424"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="495"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="431"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="501"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="520"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8061233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="570" mass="66344" checksum="6D30945538767BC8" modified="2022-10-12" version="1">MLIISISLQQNYSSNLKFKTSFRNCILEAFKRRLWKETEGDDWDIMWAEKEWIHEVLDHTHLQSNQKINHFRNHYELTRKDLMIKNFKRYKKNLEREGKQEEANNYNFFPLTFHLPSEYPIFVEEFKRQSTNSDTKTAWIMKPIGKSQGKGIFIFNKIQQISQWKNTLRYNQDNPQAEAYIVQKYIADPLLIGGKKFDMRIYLLCTSYQPLTLYLYRTGFARFTHHRYDSEDISNTYVHLTNVAIQKTSDNYDEKLGGKWNLQTLKLYLMTKYGQEKVAETFYNIQMLMIRSLQAVQKIIINDKHCFELYGFDILLDAQLKPWLLEVNASPSMTSNTPVDFELKCGLLDDVFTIIDLEKVLTGNEEQIGGFDLICKGNPIKLPINSTFTTYLGAFNNRQQQLKKIAKSTAIRLAQTYQESQTKAIEIGSKGQKEKEEKDKQMRSSSKGPQVMQNGGNGNNKPNNLTKQNTIIKRNTTNLPALGTVQNKSNIKRQPLQNAGQQQQQNQQSQSNSSKKNNPNEDSMLSANQKLQKLTQESKINQFQPYKQQSQYNEVMGMGVVSRNLQKNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6S4</accession>
    <name>A0A8S1L6S4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0350050</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6S4"/>
    <dbReference type="OMA" id="PWKGEYA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8063608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="286" mass="31771" checksum="E848E2E279DD8E39" modified="2022-10-12" version="1">MNRDLELARQAYKGKNVEMTKQAHNYNPSEQLYTDVKVNENDPLKKQDSHTEKHSTGGNYLRSSVFGGMDGMMTTFSVVTAVIGGNFGVQAVLALGIANMIGDGLSMALGDYLSTKSEQQFFKQEREREKWEVENNLEGEKKEMVELYKKKGMEQEDAEKIMNIISRHKDAFIDIMMLEELELGGAEENPLMNALVTFIAFILFGLVPIIPIIVAAIAGLTDGTTNTLFYISIAMTILFLIILGVSKSFFSYATWWRCAAETLFVGACTASSSYLIGMAFEGQDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6W3</accession>
    <name>A0A8S1L6W3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0320256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CWLLEIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="963"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1160"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1262"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1476"/>
            <end position="1494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1540" mass="182400" checksum="FD230074B8A1172D" modified="2022-10-12" version="1">MLKLFEPYYKGLIFLLSQFLQFEFAQPQIFQIVLIILNQVQPIAFNFYPLYNSEKQFEPTLQSLLISSRPYLFIVSEQSKTIVVCFCFAFQQIVITFTIYLIIKQLNQKFHEIRVSNLILQYICNYYYLVSSTLYCWLLEICMMNITKLYGIIFGTIMIIEIVTITIISDIVYESGLILRVSTQLPLNNNNVKYFIKPLRIFQIFIFFFLTNQKLCLILHNILNIAIQSLHIYQILELKIYTKEKCALTSLVIASIGIIIAIESLIMQRRINYNLWLLISLIMIYLLQYMYKRQCLSLQTVDVRKLKFLFSYIENLNGNNQENKLQMTLIIFEHQQNCLKNECICKKDINIKQKILQLIEMKFELFLEQRTKKLEQLILDLAQILFRRRQFVKAQQVIFRVFQSKDNYQSKYNQFNISITTLCLLDFLKYQIENDVLIQMMERMILTNPNNKLIADSLKQFSINENSDQYIQMKLKKTILDKSLFYYNFIEHSNKLQGDINYPIKIIKQLQQFRIELTERYQQFPTSSNQNVLKFYLLEVLNDYIEAFDLNKNKALNDEKYQLFHDSVYNKLFGQNPSYIVTEINKDLDVIIVRQSKKAMIKFQSIYGSKRKDLLELSFNDIIPEYVLKQHRTLIEQFFDEGTNKYYQQQNLSFLKLQDKIMLPIEMMVGLNQSSSQAFSFLIFFYEQMDYRSYLVINQSFDIINCSDNISNQILGNNQYSTGFISTKSLGTIFNIIPEFDSMIQSNVKNFFNHNIRLLTSNVKQAGQRYLDYVCNIIIEKKFKNENMCYMITLDNIRTNNNRNYIDSRLDTSIFQRQQTNDQNIIQKLERMKEEIDEDIDKAFPYQDIYDISLIKKVNPLNQSETQHVESKLYMTDLLVDQEQTNPMQQQFKPNQKLFSPKESMNEEFINKKNFTFQNEIRDFASGSSITMIKKSKFYKKFEIIYSLCKSLKSSSKLIKANYNFILFILLVQVYVIINLSLISNIRSFINDMKLLSVRYDVTGPLESFSVSRFTQINYREMLGRKVINSSYATQLQIFPRNNLILSFDKMKKSMGDVLLRSEIDQISNITYLPIMLYVNTNNTGQEYNVSKRSVIIITLNYQYEFKLRLDGGGGGFDNPYYYFTYKDYQIVKSMFDNLNVVILDITLERSNQEKQKWMFGYFPFLILNFIITLIIILQQIRHNNEKNAIMKYISSIDLNVIEQEKLRLSYLQQIVVQDMDIVKGFSIDIDSIDNQLEKSNIKQKSFFSKKSNTKLINNKPILGLTLSIGHFLMFAAFSIYLFLTFRIYFIKYEKTAVFYQQLSDLGVDIPAVYAQKEILFRVKLRYPFLNSTDIDQTYAVIFNALNAIKKYQQENLNLSNKDYLFDEYLIDFFTNINTGNLCEFQSNELKQQTQSFCHQVMQGNLVQGFSQTLIYILNSVVVQQSDTQNFTIQPASTLLELEGSTILTDIIEIMKDQFYKNLVDSAQSLILNQQLFCIFYLVFEILLIIFYFTKIDKYNYQQFLYLKQSVYLFPRHTLIFDDSFYRNFRSIVNEENLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9F5</accession>
    <name>A0A8S1L9F5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0340020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8062845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9F5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="468"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="574" mass="69286" checksum="203A3BA498E6C1ED" modified="2022-10-12" version="1">MKQSIISPVNQLNSPSISHFRSQTSGSGVFDQSRILEETTEKFQRSWMMLENVEQLKSLRYTLKQTLTEPNLHQDCQLSGTLISGKVYKVTPMEFYYCNIPTKGYKSPLNCTVYCDGEFHIMISFNSPFPTKFNCDQVIRNRTWTVRHNGEEKLNLAFVARKQTDIKFMIYYGQTDSFKKMIRISSNHERVQKNEEIQKIPQFIVNQNKIQAFKKVNKYEMEVSRSDRFLQVLKTRNLQKKAIVEANQQKNLDFKAKEWAHDEYKLITLTLRKRERRTEQTFLWCELLHYIKCVDKLYYMLQQRRKQLKKNKLMIISIQLRIRKFREDHKLNRENLNYRTIGQTMMALKMFCRQSKRRIINNSLILVLPLLKQRANLYMFKKKALITSGKLQLIRVNLNQFVKNVREYKARMIQKWDQYTMKIHQYSSLYKTDKRFITWVRILYEKGYQIYFQNLFITLLMRDRYRSHIKEQREIMKYRQEMKTAKLQLKFARETVDMLTLRQRIFKLSNDIYAMQLKNQFFIHSDVERFVSAIIEQSSFIFQDEDSIQIGNERSSRLRVSLKRQKTLRISRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9P0</accession>
    <name>A0A8S1L9P0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0330119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9P0"/>
    <dbReference type="OMA" id="IHETDEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8062403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="52" mass="6093" checksum="F70501C1A4CC6BD4" modified="2022-10-12" version="1">MRKEYFSAILRQEVGWFDSINPNELNTKVADETFAVEDAKNFQQLVDGNQVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBQ3</accession>
    <name>A0A8S1LBQ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0350088</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LBQ3"/>
    <dbReference type="OMA" id="FMVDILN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF4">
        <property type="entry name" value="NUCLEOLAR MIF4G DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="4">
        <location>
            <begin position="618"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="175"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="183"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="206"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="296"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8063685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="825" mass="98895" checksum="B1192EFD13100230" modified="2022-10-12" version="1">MVKKNENLQQDDHHERFQQIKPEFNVFNRKKLRKEKKMIKKQKHMQINQQRNWKNQGVQNVNQNQPQEKEKKQNQEIKNQSTNNQHKQQQQQKLVKQKLNSNKNEIDEKKKLQLQREIEQEEKEIERLTKKLKLKQNRLPKAQILEDGLEEDLFDFLDHIDQTIKEKDAPKNYLAFQKKNENESNEDVSVEQNLEDQSDDQIKDESQEEIEEEEEEDEDEDDREDDEEQNEDDVDMFEDEEQNEDDDDLIEDEEQNVDDDDDIDDDEIQPDEEEIVEEENQQSDEQEEEEYEEQNENLQKKIQQKQDQPINEQKINKVQKEQLVQEIQPPQEIQYDETQLLEIKKLLNRNLNKISEANINNIVDEIDKIFNQHPKQIVGLVFGQQIVQSTIKPIELLPYLLSCIIASSCYFHQVYDNLFFGELIKQVILKLRSSPANHEVKNITLIFCHWYLFESISLQFIKEFIKYLLKTQTADNVENLLIIIQYCGKKIRQDNPAILKLIIEDIKQAFQNVSESRTKFLLMTLDDLRLNKKIISQSERLEFILNWHKQRTQKLIHRIFCQGFDFSYESQFLSNNWWKPIVSNEEQSQLFQEFKQQKRNIKLEKLEQLAIDQKMVTETRKTAFVIIMGADDLFEAAQNLDKLGYLKKKKQDVCLVIVEMCGNEKTYNPYYQELAHYLINLERGMKQAFQYMLWDKFKLLENYSIRKLINIAKFCSNLLKKESLNLLLLKWFNYDELLEIQAQFLCIVIQNFLQNISIDIMAKQSKNLFENADYLPFRNGLQMFLKNRFKSYIDSQDDQNKFNQLKKDLRIMIKLLDEDVINNDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBX6</accession>
    <name>A0A8S1LBX6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0350134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LBX6"/>
    <dbReference type="OMA" id="PSIYMPD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="338" mass="39944" checksum="8134B6AA01D2E90C" modified="2022-10-12" version="1">MSSALSLAGQKWKEFWGISKTQNIQLEDKKKQINEADQYIRLAGRAIQGIVFQHQQMQLLYGLLSQVLKKLDELEKSQEGLLLAKTFDSLSSLHSSKIESIHKANDSFQEWFDTIEQLRQMLDKYQENRLVYDHYRLKVDQLKNSKDQQRFQRNEKKYNNANTQFQGISNQIIQKMDQILNNKRNMFNKAYQIVIQNETEIYYQAYKKLIKHEDFKPKFGYDNQKQQIDIDDTKQKQQNDINNIECKSDENISIKDIPAQGQDWNPFEQNNKSPYEDQLAQSQIVQSYFSQGAQESVNPFESGDVASFPSIYMPDQKYQSNYKMDYEKFKQSTKQPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCL0</accession>
    <name>A0A8S1LCL0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H(+)-exporting diphosphatase</fullName>
            <ecNumber evidence="2">7.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0340227</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.3.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCL0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009678">
        <property type="term" value="F:diphosphate hydrolysis-driven proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004131">
        <property type="entry name" value="PPase-energised_H-pump"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31998">
        <property type="entry name" value="K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03030">
        <property type="entry name" value="H_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8063253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="427" mass="47107" checksum="4BC16E22DFF16AF9" modified="2022-10-12" version="1">MLISLTEQRLALKINIIISRMIIKIIILNFCQKQHLIQKVEQLQFLAAEYRYIGIVIFFLALLIFFIVEPVLGQDWNTVAFLVGALTSIISGYIGMRVATFSNYRCANCAQTKMTDAFAVAYRAGCVMGFALVSFALLSLTILLGIYINWFIKDQRDFQQLFEAIAGYGLGGSVIALFGRVGGGIYTKAADVGADLVGKVENDFKEDSPNNLATIADNVGDIAGMGAELFGSFAEATCAALVVCSVFPSFYYHPTTFYYPLLVSASGILVCFIVSIFAFLGEKEDFDQVSNVLKVQLILSTLLMLPALYYVAYLTLPERIFGLSPVDRQPIHAWLCTVVGLISGCIIGFVTEYYTSHSYRPVQEIAQACGTGAATNIIYGIALGNLSTIIPVFLLAFTAFISYSFVNVWSFLKCFRNVINFNYWFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCP2</accession>
    <name>A0A8S1LCP2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0380111</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCP2"/>
    <dbReference type="OMA" id="WKTKYEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="134"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="319"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="61"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8065818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="588" mass="69462" checksum="C6238A009607508F" modified="2022-10-12" version="1">MIKFQNQPINYDVNGYTQPKYSQMPPKQQKQIPNQGCFQETFGQQQYYGRQMIPQSQDSKNYQQPQQHQQQQTQNYQNYSQKQQYPNMQQQQQHQQSLQQQQHQQSQQQQLQQQKTDPRQNVQFTAKEFNQLFYKQQQQQQQQQQQQTNQNKANENQEENKYTQPQKVTGNERQISTYSQNQQQLLQQKQPGIPGRMQSADPVQAQNRNAVPLTNIQNQQATKTSQYISNGGINKTQNDYSQRQATPMKDQMKFMNQKQVPEQINQQLMYSPMPKSQLLNETTNLAKLQQQQLQQNKSPQIQKVGGSGMGPALSQPTNAQQIVEELAKEKERHQLLTAKLKKLEQEKKTYNTQFLNEMENKIKTLIQQNDQLQNLNMELLQKAQDQPQGEIQELNKQISYNSQQLDFLQKQINEAQKQLSEQMKKYKDLEQQYQEKNQAEEQQIQYLVTDLEEKVHGLIAENERLNSLIAQQDSNPKKEQLQQLTNEKNKLISMTQQSQKEVKNWKTKYEQLQQKLQQADKFLEPQGEQVEALEEKIKSLIEENDYLNTVVQQQIERENQIALLEEEMQNLVEQNDNLEMLLQNSIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFT8</accession>
    <name>A0A8S1LFT8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFT8"/>
    <dbReference type="OMA" id="QEFKNPR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="139" mass="17031" checksum="B046C37D0EB5BB83" modified="2022-10-12" version="1">MPNEFILQQTSHPEKKFNSKTLIGNWYEERCEPKSKYSTFYKEQKFEKNQYNVSKLTQESFMKCSQNWLNFQKNQQNHFQTTNQQEFKNPRDQFRTSEIKKFIIKKGVFEKNPQLMSDYRTKWTSAPHFFDRTYLGQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYR7</accession>
    <name>A0A8S1LYR7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0490064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYR7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="105" mass="12166" checksum="60DEE4551CDA3892" modified="2022-10-12" version="1">MFFQKKDCFNNQDIYLFIVLLVCLKSQNSKKPILKITLDMMSGASFCKLDNPPSFKYSQMIVSSNNKTTTSSKKQSLKFQVIFSYINPSIFYEGEKKCKFGLIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZJ4</accession>
    <name>A0A8S1LZJ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0510046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZJ4"/>
    <dbReference type="OMA" id="VIEMEMM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="203" mass="23879" checksum="FB631913B776C396" modified="2022-10-12" version="1">MQAKSNIVIEMEMMIESTEHLNNLKGDSHKDQITYMSGQNQCIKQKVSQIDLEFQKLLFQLIETKLSENTISQVHDLLKRLSSSLIIEIPKGPQILQSLYLIILIRKPTIKRNFPFSPLSIILSRIKKIIKRVKKLLQRKLNWIQKQNDIQIKQHSSFHLYKSQCKNTDNQIDCLTRQLSFEQYKEPIYKEITIIQGQGLIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0D6</accession>
    <name>A0A8S1M0D6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0470063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0D6"/>
    <dbReference type="OMA" id="ACHACSI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="24070" checksum="B6016277468BD281" modified="2022-10-12" version="1">MTIITINSEFKLQKMRLQKSETPFFIQEMLVKQEDSYEGAYVNGQKHGKGLQKWAKGDSYNGDWVNGKRTGKGHYIWANGDQYEGDYLNGQRHGKGQMRWRDGATYQGEWVGDKRTGKGQMKYVDGGLYDGEYLNDERHGKGQYKWANGNQYYGDWVNNKRTGKGQMKWADGSLYDGDWVNDQRHGKGQFKAANGEKYDGQWIMDKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9J1</accession>
    <name>A0A8S1M9J1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9J1"/>
    <dbReference type="OMA" id="PIMPKIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="231"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="616" mass="72617" checksum="3D33C7DFB52CDB27" modified="2022-10-12" version="1">MKTKILSLFLKREHLILLSKMLQLSENVIESDSQILKLDIQNQQSENKILKTERWPQLDLDYFQNNFLQIANLSFSYFKLHFPANSAIHIKDHIKFIQILKQINYGGVTIEDVFKQQRSRLQIEVSKITKSIKTVDEPIMPKIMNYKIDLEQIQTIQDKYFYPKNSDFLNPYLILNLKMPFPKSTARPLNKELYQSITSLEDVPSFQFMNSTLIQPILNYDMLKNSFDYRMNKLMQRNQQIQYTIKRYQQSIKNYDNINDDIQDSSLKEIKKIFCSSIKQIYDKKNKDIVLEQNKKITIDEQISKQSSPHQTEVFKENDSLLYKPIQEDEQQYQEVVTCTNQNERSQDQLPDFDPESPKLHSLRLTPYGFMETTYQQTVSQKMLPLDHQHQLTQSPLRAIPKYKASYKSLDLQQSLILSPSTLKYPKKIFAYQKAQKLNQQNSQNDPNTKVILDLPIQTSENLEQSIETQTLDTQKAILTNRNTQKTCLDKIPLKPIAIQQIRLKKLKDKLIDKKETTLPYVRESLPRVQEVLMFQKKNNMKNQLQYQLILLTRSEQILTSLMSNGQIRGTELYQQLLQPIKLTTSQKFLSPKSNTLISRTKFKKIQQETKMFNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBB7</accession>
    <name>A0A8S1MBB7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0580263</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="VACINIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000525">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000310">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000082">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000117">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF62">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN 60E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="471"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="541"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="569"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="636"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="742"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="780"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="807"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="870"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="976"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1051"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1117"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1162"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1267"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="96"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="448"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="740"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="1053"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="697"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="713"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8077738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1488" mass="173287" checksum="C06BBD03B63A3CAD" modified="2022-10-12" version="1">MIFQQLKITLPPIGSQEIEKSIQKRMGDCLETQLGFEYEPFTFQYLELHPDLVTIKNQKIEHHPIEDQQHVQTALGCFSKENKIRLFLSENINVLQRLVIIMVIVNTIAFCLYDYQIRDSVNNLSNRNIASIIIETICNVFFGIEIIIQCICYGAIFGKGTYLRNIWSCLNFLTFVCTWSIYFDQSITFIQLLRVIRLLRLVRLLQEIHYFREQVETFFSCFLTIHTIIIPLVIVIFGFSVIGLHLLRGVTGYRCAKDGIIDLNIRNLCGEWECPDGYECINHFNSEDLNYLDFYYGYYNFDTIYDSFLSGFMFFNATGWSPTVFYFWKAVTPPATAIYFVFMLFILHYTLSDLLLATLYESFLVSSAIKNSIKSSKSELFTQRRRTVTLINNQNKAQINFHKLHMISQISSQNRANLVTPAEILRNETLKEKDKSCIINRIKQFDQLIIIASTIILCADQVEKRNHPKEYYADMVMNFLIIIITLLKLILFKKSRRKLYLIVDVLLSLALIVVIVLEFFNFQSNVFIIIKAFKAFRVIKLIYKLEYFGVIRLLLRCLIETIIKIRHLIILWVIFAFLISIIGQELFAHYVKGSNEIEIHFNGIGNSLTAVFNIFYAEEWHVTMYQHALYKPVSSFIFFLIVFFLCHTLFMRLLRALFLNEFGKKLNELDQKYPQTDYLQRAWQYIKNSCFQQESIQSSSQSQNEDDSKAPLQETNNNGISIKPNINQNKGSNYKRIVEHKIFRISILLVVTLSAVKSAVQNPLTDPKSEEKMILDIIQYITTVIFIIELILNCLAYGIVKFINQSFLNILSIINIIVNLISIGLGNPPLFILTLFDSLRVLAFLKTGADQYPILKQALQALFNAFTKMIQLALFSLLILLMYGIIGMQLLNDEFYYCSLPEGNSLELNLQSKIDCFDIGGNWINRTLNFDTIFQSINILFCVATSEEWIPLMTPAWQASGIDHQPHHDTNRYWSIYYQLFFFIGNTLVLGMFVTLVVETYIKTREESQNLHLLDDKQREWFQIKEQILHLKPKKKFRPPTKFILNLLYRLYQFLQIPFLIVILSNIIVLSLYHARMGQEYEQVLDKINQIFIFIQLFEILISSVQITDYKFKIYEIIGIVLSVISSFLDYHILHVVSVAFQVTRIYKLTQHFKSIQHLFHAIFAVLPNTASMLLIMLIVLYCYTIVSCDLFAYLRPQQSINGFDMHFRDFWGALMTLIKVSSGEKWWVVMQETTLQQSPAVACINIESYDEFSEIGFNGCGSNLSYLFFISFILVFSLMILNLLVANIIGAYEQYHKSEQSAISKYQLLDVMELWSQFDEDGEGFISYKLFWRLSSQIAIIFGLEQSDLLDVNNKKKFLKALKIPIYELVDSNVLCYRFHDVIVSLTRISVTIKYGVVNLEPEDKDIHQKVYGNKHSHVEPKFRETTLNSGDMVSIIFIQQKFREWKQRSLLKNTIILGGIGDYRSLIKIKSKTLSEKIHQQASNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBF1</accession>
    <name>A0A8S1MBF1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0540016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBF1"/>
    <dbReference type="OMA" id="CKFKIYH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="78" mass="8698" checksum="D7F4F4A5F0BAF6F4" modified="2022-10-12" version="1">MDESGIIGQMILESSGRIKKSSGTLDNPKLIPFITTIVSSTAKILDKQSLNRVTLSYQESDIIIYIDQTDIVVLERKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MH97</accession>
    <name>A0A8S1MH97_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0570115</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MH97"/>
    <dbReference type="OMA" id="QNGFDTW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021622">
        <property type="entry name" value="Afadin/alpha-actinin-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11559">
        <property type="entry name" value="ADIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="115"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8076905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="416" mass="48856" checksum="18411E61DE3466C3" modified="2022-10-12" version="1">MFSNTYEQSIIFSDKVQMSSFLEKMVDEIEDYRCDCKISESCIVCETKKWMQMLQQFNGILNQQQMPIFPEDNYNIPLYLSKVYEIIHAVFIFKSATMKSLNNHEDEFFKIRSDNESLKIKAKRFQEDIEQKIKQIGQLENQITLLNKSNKVVNDKLLSEKEELNQKVIKIQNKVQLMQSEFKKKELEFLKMKDQLRSKDRINYANKYDTIPLKTQISQQNGFDTWISTRKGLIDEVCSLQDTLSTLISILNHFFQSLNNGSDFFNPDTIRFPQQSGKSILWIKQQLQKLNGQFNIDDSFLKDLPQLNSNLISSEQQQQQYLQSIVNKQQQLNLSHQKTNSINNSGKKDNLKIINRSQTLHFQQQQPLPDETISILSRISINDVSLVESTNSAKFAEKPLQDKQQLKKWEFVFKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MHH6</accession>
    <name>A0A8S1MHH6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0570155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MHH6"/>
    <dbReference type="OMA" id="EIQCEDK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="322"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="371"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8076985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="496" mass="59050" checksum="8C97B1C1BC95A01C" modified="2022-10-12" version="1">MHTRHSKLFESGGETVKRNLLESYSMQNRKPNSNEEQQLREQVDRLTNELIILKNKDKAKVSVDKQIQCEDKRWELLNEIIKQKDKSIEQYEKQILSFKNMILQSNQQKKELLLKLEYQDSIKKTQMIKVSQNIQTDLNNNKVQQTQQNEFDKGIIYGMKQMDSPTTKIKEQISKLVQTDGKFYYEIDILKQDEIYKEIEQYKLILKDYDLENRRLKETLQSSKENIDRETQITVQLENKSVQVGEEFRDKMDKMVQEYQISIDNQKQLQKQYNQQQIDLNTLRKQYDQLVSDYYFIQSESKVNEKKIKELNEEIEQLKNELQLKDPSVSSTETMTSPKDSQKSSKKKGNNQKIKSFVQSNYFNPQNVDELILQQEENQKLVIKIQELRRELVETSKSLKAIQEELAIKELILIQQSQELLQTQTELKNIKIRIESVDILEQKLKEQELRYTDEIQKLSQKLAILNSDYLGMKVINQTLHFQLDKYQNKLKQQQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MI31</accession>
    <name>A0A8S1MI31_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0530228</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MI31"/>
    <dbReference type="OMA" id="PKPLICH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000465">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="165"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="198"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="231"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="395"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8074934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="702" mass="81932" checksum="8F21AF85BB5BC5F4" modified="2022-10-12" version="1">MTHYKNQSAQIILKKCLEKYLCFLKLKQLLKRFDLVSKSLEQFDVYEQNEFATQNSQQYSQECYEAFQKIYQKNEVYQLQIIPEDEKEFYILNRDTGLMIDCRKLDQYTVPLSDTKEVAWKNYWKDSREISEQLLSLVQLNDKNKVYELLAHPKIYIDINIRDLDDWTPLHFACQQNNTEIVQLLLHQQANPRLFSLDRKSPLHIAAMKNNSTICELLINFGADIEAQDSDQNTPLHLASLFGNDLVCAILIEKKANHNLKNYQHLTPIEMASDIRIIEIFNKYGISLNNFTYTRTVVKEQNLILPNSRRDHVQKILKLTQQQTKACIIDQEIKKELINNEQQRQSLTTLDINQNRTTWGKIMDFAISFSRISIKVNTNTTNSESQNNKIGPNSFQFYQKLGEGGFGQVYLVEKIGQEPKKYYAMKILKKEDMETSNIMKSAQIEKDVLKIMNHPFIVKLNYAFQTLDHLYLVMDLCPGGDLATHLELVNHFPEHIVKIYAAEITLAIEALHQQGIIFRDLKPENVVLDKDGHAQLTDFGLSKQGIDEEMLNQSFCGTLAYLAPEMLMKKGHGRQVDWYMLGIFIYELLVGAPPYYDAEKEILKENIKRAPLKIPRHLSQEAKDIIIQLLIRDPKRRLGCKEDAKEIKSHPWFNDINWQDCYNKKLQPPKPLICHEPKVARKVTFSKNSNRNKLNDWTFFEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MIA9</accession>
    <name>A0A8S1MIA9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">MIR domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0630161</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HEFAYLS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23263">
        <property type="entry name" value="beta-trefoil_MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13715:SF99">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13715">
        <property type="entry name" value="RYANODINE RECEPTOR AND IP3 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2502"/>
            <end position="2522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2555"/>
            <end position="2577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2612"/>
            <end position="2635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2692"/>
            <end position="2710"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol 1,4,5-trisphosphate/ryanodine receptor" evidence="16">
        <location>
            <begin position="10"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="14">
        <location>
            <begin position="248"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="RIH" evidence="13">
        <location>
            <begin position="1365"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="domain" description="RyR/IP3R Homology associated" evidence="15">
        <location>
            <begin position="2048"/>
            <end position="2144"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="12">
        <location>
            <begin position="2568"/>
            <end position="2719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1792"/>
            <end position="1823"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="2812"/>
            <end position="2842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1792"/>
            <end position="1809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1813"/>
            <end position="1823"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8080237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2847" mass="336004" checksum="EDB3785968E881AE" modified="2022-10-12" version="1">MEASEALLSKALKIGCTVTLCYKDDDGKNYFACCEGFTSMKLRLLPASKIKEQGRLNQCLFKVVPPFQFVEYNKGKEAFHQKETLNQVTNVDQRDSRLEFPKQIKLMQDESLLNKESLGKMKEQSIFFGQTIQFIHQASNKYLSYLQDQGSDYESDSWKATLKMEHGEDTQFKFQASFSFQQETGGLIFDEDTVYIVSVLDKSIEPGLHASNKKKDKEKRELNVAPEKRSFWKINIFSKFMKDELGQLSVFDVIWINYSETNLNMIVQKYSPDEKKKYLKFEKGQDSEQINQFIGDSNGMYVIENIDPEKGDFLNGGYVEWVKEYRLKHLTSNYYLKLEEFAENQGSFRLVAVENIAESSIFKFGMIYSTIVKKDNQEFLTKDSYFRLSSRDQDNQYWLKIDTVGPNDIYKIEGDNFIEDITIPIFDQDKKDEHVFNLACASLNEVRFIKYLLSCMKILKRSKEYLDVDLNNTNFFKESQFQDIYKKLVVRSQHLEQVLDDLYLLCHNKLEGFVSFDAEFGFPHQHTQNLLKEQYFFEILIQILVICFPTQESLQKADEPVELVQVTKLLSEEQQVDQNLEKQIIARFVEKKKSICTKIFKLLQAMVVDNISNQEYCSKYLPILSMQAMYLNGAISTIIAIIHNFEELLLNLNKDDNPLDMPQQILKAASIKTPLTNQNKQKKRRRNEINKPIIDYNTQYKIICKQIKDSNHNQLQTWDLLMFFTTMLKSNNFKFNKKIEIMYFLKESVCFEEKGININQESAYKYILSSQRVFEDEILIRLKSDGPQLKVYRGDQESTLLDYYHKYSNNNDPRLLNFIPYVQELMRMYSRMCNSRNYTWKKFAENFFNVESLIENIQNNQYNVEIRSIICSLLNKLYVDQEPRRIIIWPELCKIVRTNRKQNKNDINNTMLSEGKQQNKITKTDPVDIPKVQEKINGYLADQLIFVQNQSDLVELSKGPTERHEMLDIVFKNAPEIYNQLTLEVIKLFKLLIQFGKYSIVIEEDEEKTIRKNQQLFSNIRALTAILEYNSTYPEVRKILSIKRKEEENQKNSTNLFGFGDKNKKKQVEQGQVKQQYSGEDDAEDLSEAENFQKQVEFLSLKMNQFMYQQQLPNDPLIKLEVCEILDLYLDSKMDFYLNNLKLQFSQWVDKLNLFNEVLNSNLDIDNPQHKEKIQQQFRDTIKKNLDNMLPAIMKTGTKVDDEIFKKKDDNQIFGLMNIANIANIANKLVNTDDNQSKVKSLDAIELSNIIQFSIFPSLISIFCLNQSLDLETKILHILTRFYNQRHEFAYLSSKLLLLFDDTNITIFKKSKKKILVLAKCVDESETWVQNLDEPQNLKTLELVQQHFEFFTQLLFKGSYLNDAQELEKGHEGQVDSIRQDMMRHLKVDKLGVDLIKDSVFMVEQEDIDIELQQKLKKMFQVCLTFLKYFCMNNLTNQNVMSQHIVALISKLNTDLGQVELLCEIFRDNKTVCYERSQEVIDDFVRLIITNGRRVRYLEFFLVIMQIKTEYISQNQKAVLNLFIDPRYKTQLFYMLPKAETDEHPIFDFETKHNENPNYKDEPYDYHTKFIEVCAQCTIGKEGLLSSENKLKAQITFKYVMELLLEKDEFSEFIDPPSGLESEQQFMEVPTHKQRSQLQSGISIIPLNQAKVEGITKMKPSLLDLLFYTYLESEKISEEVFSCFDMIIQFIANEKDRIMRCKNFIPQFYDYFFSNFMLVLNGLVKVLNRELGSQALVQGSKLQKQSTEFYRELEELRNFLTDSFTSRLQLSNRQKTLLQYFKDNIIMKEELNKQNQDDQRYQQEKERQKNRSRQASQYKTPKRLQTIQTSKRELSTDQIWKELWDIFLTEFLDSEQLIEAINKEQMAFAQAISKVEEIFMKDKIVHQQINKQIIFQRLIAFLEKSSASADNITTFKLILNVLRNFIHIEDESEFTPDMDEEEKKNIVQRQKKDNQNFLNSNNAMQMTLTLLSDISDPINSQKIFSELIQFAIDLLDGGNQDIQRSIWNYFQNFTESETIFLRLHNEFQKAIKNMKKTDEMQQYFGFQKQPDLINQILRLLQLFAEGHYRELQEYMYFQKFNHHSHNLIQDFIDLLQSYMNAVEPEYFENMVQNFDTITEYIQGPCRTNQKALIEANFLDLANKLYEIDEIALEIQDEIIEDEEFLDEDNQELFTIQKHLPRWMIAFLKYKCSITLISLLEDREGDEIIQRIQKSLSNENLKQNINNIFYMFQIYSNEIYDLKVFGHFVNSQPKTQEERTNCSFIIETGFNLFFIYQIHQKTKNKTVQQSKNEEEETDDVTQQLLNSPFYKLFLLVQNLAKSLKDSVLQVAQIYKKTEKQTPKERMQKLQLARQQLFPKAYKFFLSKTAKIEIMLESKNVEETYFYIPPYCQLDDDVKRNFNETANRNSNKAKVTSLVESSEEIIKTMKLNYKIQVYLNQIKTLQVIIENIDLLRDIEFIIALLINVFIFLFYKKKYVGIEVNPDLTETEIWLDDDSFEYQSLIDSLGILSIVLSILIVAFFLSRNAPLLLEKAWSGVGQEKLNPIAYLIRLLKTVYYLLSDFFALYYIMYGTAAVLGRFVHNFFFSFHLFEVMIRFPLLLNVVKSVWNPRKMILYTGLLLLIFMFVFTVFSYRIFYESYDAGFCDSMWICFLSTLDSAFKYDQGIGGFLKNPYEVYADDEVKLVLRFIFDNFFNILVIIVMLNIVAGIIIDTFGELREELQEYNQDLENLCFICGYDKETIEKESINLQNFSDHIKKEHYQWYYLFYIAYLREKDPTEYTGIESYVADKLDAVDITWFPLNKALCLKKDFGNEEEKNIVTSLEAIKENAQELKNILVEIQTEIEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNH9</accession>
    <name>A0A8S1MNH9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0600228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNH9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8078743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="53" mass="6368" checksum="E5AA0D2D15B90653" modified="2022-10-12" version="1">MINNNCFLIQKLYNIIILLCFANFSTFFRLGKLKEKQMIVSDKKIQKILRLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPK5</accession>
    <name>A0A8S1MPK5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL15/eL18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0600209</name>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0810168</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPK5"/>
    <dbReference type="OMA" id="KRVECAK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="6">
        <location>
            <begin position="72"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8078705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8088565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="147" mass="16615" checksum="6C57E1B8A75A2BD7" modified="2022-10-12" version="1">MVSRLRKCRKMRGHVCHGYGRVGKHRKHPSGRGVSGGLTHHRINFNKYHPGYFQKTGMRHFHLKKNTLHKPVVNIDKLWSLVTDETRKKYASSKDKAAVIDVTKAGYFKVLGKGRLPNQPVVVKAKFFSKTAERRIKAVGGACILVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQR1</accession>
    <name>A0A8S1MQR1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0680160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQR1"/>
    <dbReference type="OMA" id="QPNEFFE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="272" mass="32607" checksum="5A62704F7F217621" modified="2022-10-12" version="1">MYYYNQRIPVPLQRIEFKHILSGLQKHSQSQDEINQRPLATRKRNIKKPEVKFLQDLQRNEKHDQQLLQTTDTMWDDRILQKLEKLKKQPNEFFEKIPKEPITLMNSSKINKFFEIRLLAQSKEDVQKGSRESQRQLNQIQEVAVKELLNKYPHQFNFTTSADARKKKEVLDTKDKYMKLFFVRNCSTSMSKSKVNNKYLHNSDDQQKYMENIEQIQEKQKNKNNLSFCTWNKLQELECPGYCSFIKQLAFKNKLQQSCSQQKIDSYNLVNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MRE7</accession>
    <name>A0A8S1MRE7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0580116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MRE7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035723977" description="Trichocyst matrix protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="40317" checksum="5B3150937DCBEF0D" modified="2022-10-12" version="1">MQKLAILTLVVALAASKFVDIHTSLAQIDANPFGNVVLSAIKAHIQAQTPANEVNMLLNGVAAGLQQDQNDHDHAFELDTTTNNRIVEDLEKEILYHQNQISSNTQLRDDTIEALAVSEEDIRVTIQDIATNEATYAREEATRNQQHETFVSKIAAIDDVIDAIDDAAKLIQHLSLGASFVQVKSKYETIHKRLTDNTSHSQLLQPVVAALTELATHGVNQKALTKIAQLLSEIRQQLVQEKAVKTDVEDRQAAHWAEFSAHLSNEHTRLVERKAQLEVQIQEQKDTIEDAQSWIEFHTLELENSEERLAGQQAWYAVQSEIYETQTAERAAQNEIVDRLQEHISEKLSTTAQFISKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MRZ6</accession>
    <name>A0A8S1MRZ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0580225</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0580226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MRZ6"/>
    <dbReference type="OMA" id="QMMMQQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8077664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="294" mass="34075" checksum="F3ABE1FBBAC28E7D" modified="2022-10-12" version="1">MELIQEITLLKESFLEFITKVTPVFTEPILEQFNSKWNPLKRLLSDPSKSASDVFILVSSMSSLKKSNTSSTSATSPNSDCSIQKTQTTDNSVKKCNELPEQREITKVKEQANPLLYKELKIEINKNFFLNTPRSRPTSQQELGSNGERLYEEDIKAKILRDDIKFLNQLRSATPEKKISVTVRFNLGQKLSEAICQFRKDNFTLKTIHERSCEVTTPQSPKKDRSESPLLLVQRPKNQYFQQPVFIQQQQAGALKGYSIYSKKGYIQPIQFDVQFQMLMQQYQNNMIYKKLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSM0</accession>
    <name>A0A8S1MSM0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0670186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSM0"/>
    <dbReference type="OMA" id="FNIYWLK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="607" mass="72937" checksum="D21071E374A35CA2" modified="2022-10-12" version="1">MNESITFLFQILETNSGKLFQIEKNIENTQTKHYALEDSKQVISSQYIIQYFKSLKESLHYDLFRQFQGMLLYQEKQYYLFDYYETSQFLDQMVIDKGKIKESFSYAELKLFLKNLLIALYELHSRQIPGRLFSCNNVLFVKPNDRLVLMDFGFGPDIKQDNFDILAPPEYLKQIIDKNENQKDFDLKFDSWLIGSILYHLIKFRSINHVEISINQYERYKYDKIEQYYKYLNSIEFIPCKTTRYKENLLSFVEALLTCNREKRLSFYQIYQHQYIQDLKLEKQQEYIEFYKNCEYIHNYQEGIIDSFDLPIVHSQYTSQRKTPLIIPAESQIIKNQKSASPRSLNPKIRILSQHKIDINKIPNFPDYLSIIMNTPQFQHQSYYTYWLQIQLDCLKCYLLKYTAEKINTVLPKYNHPYINVLVYIVKKMHLIILREMSNYLKQDCFFENIKDQNWKSFLEQYEKNLPTSDQMNSYLNELTNETIAIFEQYCSNFLQVDSPIHNTTKQSLISVTVQNHNLVLNSQEFYDDCYKFELEILFQVLNDEIIKNSQDKELAQLKQLTYFCQQICSSFKRDVFQKEFKDLGKQNRQVQPQDIINYLGINNQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MUD5</accession>
    <name>A0A8S1MUD5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0690219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MUD5"/>
    <dbReference type="OMA" id="IEAQCKS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="27109" checksum="548FFF3913549265" modified="2022-10-12" version="1">MKLEIINIQTDRIKIAVNKESDKETEQENIYRFIQDMINSGQNDVAIMMNLQLALKISQDEIYQMKFQHIYKEGNNCIITFNNKKELVLNNEHLLSELQLIFKVNIGGKVSNKRNRIIEAFNQTRKFKLNHLGNSENDKQYQQFLQQISLSKYKMMDRSNLRTFTLLDNSLQNLQSQKKDKKEEKLRKHEYTLYDKVFKRTMSGDLIEISNDQKSPIEQIVKLGLQQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWH5</accession>
    <name>A0A8S1MWH5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWH5"/>
    <dbReference type="OMA" id="RSQYKFD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="329" mass="38571" checksum="986E7282906DB142" modified="2022-10-12" version="1">MQRKESKKATIPKMMARVLQYQDASDKLTQFLFIKQGQDRIRRIILAFLIADFTNLILVSGQWYVGFHQTLKEWLEDLDNRFIKAHLHILSFKNSDFLQTSFCVDNTKTKKLFRWDRTIISEVLNGFNGKCITIAFKYNRKYRSQYKFDVLPSNSKRVIWIAREQTKHNFESVTQVMNIQPIISGDCVKIAINFYNKMTFIDPDTIEFEEPQIEQSKECICPIQSLFFDWVSIQYAKQRPQLNDYQVHPHLNLIDCRCAGVDTVAYQFVYEACELGSFRNDLIGIPIEVVQQGQEVVTELNKVGLVSDRECKLQLRKQDQLIFYLTSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1K9</accession>
    <name>A0A8S1N1K9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0780043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1K9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="1">
        <location>
            <begin position="28"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="242" mass="28016" checksum="3C20C7A9DA6CFA7B" modified="2022-10-12" version="1">MNLINQKIVKAGLIELQQFRLRFPSLYVLDKFQVEHILSVIRNKTTQTKDFRLYSDRLIRLEKSVSEHNKKILPQEGQQTQQQKEQVINFEHNQFCVVVMIRSGNAFMGEALKVLPVQEHPQTKDAQLIYCKLPDDIDQKQVILTDSMITTGVESLLQQRHCYQIELIKIILQQLTYFQQLNQLIDSTIVSCEKGLSKVLHQFPKVKVITAGVDHALNTIQDLRFPGVGDFGDRYFGTVDQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N3B7</accession>
    <name>A0A8S1N3B7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N3B7"/>
    <dbReference type="OMA" id="FKTWRIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="519" mass="61190" checksum="1B09AD776B3DBA4C" modified="2022-10-12" version="1">MIGKDCLIKKFKTWRIRTQILVTQLITMIVSVTILGLCLFLNFEGIFQALQNDAEKKFIMNFIYRQTNSQLNAYTRNLENRIRQGIYAINDINLLNQQIYNTSMTFVQNSDRCLQESYLNESFVLKCQYCYGIFDCSNQIHNPEQYSQEFLQLSNLLTSLIPMQIQQDMYFTSISKDCYFSTYPGQPFGDYVPSSRRWFQNHMANINKSEYIISEPYTNYFGGVYISGTTNLYNGANNERIGIIVVDLNFSDIHQFMEDDQQMLAMDSNGRILISKLYTVNTSEVYYLYNQTIFGFDQQDVNLILNHDIQQQKEQICEIQIPQTICIENKNTGVLWYFRVKKVTGDYYVLSMFNSSAYSNYYQTLRITLSKIYTEIVTDFIFGLLSIILSCFCIYLGCFVILQKPIDKLINSFNKYLLYGKQLSYLMLKIDHHDQLDKLSQAFIRVMTRSMSNKDNRKRIVDKYMSESKYPINFYVNSKIMNAQTKLLLYNLEMSSSRQKNIDQFFQQYRQENSQQKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N3H7</accession>
    <name>A0A8S1N3H7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N3H7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="45313" checksum="142BC96222F5082B" modified="2022-10-12" version="1">MINLKSICLITSVILIIQISFIFEPEELYIPFQKQFAQVPDFYSPIVLLDLKNKFRNIHNYYIASPQLKIFLNINFRVQSQNSLWFAFNDDIVQVINNFNIITHPQTQYAHSYQKDCQENLVLFNFVINYPIGSQYYYLIGLGLTPETLTISFQFYSLKQFGYHILLNSFDIFLIGPPILIIKLDGLGQVVNFPTKWTIQNCYLNLLGFKHKRTDFNIRYFMKTTYSSQVTFGIYPCNKFLIYFGVPLIIYQFNIITFVQMKLFFLTGKNKQFFDPNNQIDAHIEIKKINYSQNQMLLEEITIAQSIGSITIILLAIYRFGNVQDITILSKTTVFQTTIKCDIKQIHRVRLSLKLLQNQNQQQQIQIAKISVSLFATQLTNHKLEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4L0</accession>
    <name>A0A8S1N4L0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0790072</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4L0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000833">
        <property type="entry name" value="AGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000735">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="17"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8087708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="467" mass="55024" checksum="6A0A36AD5EBAC31E" modified="2022-10-12" version="1">MNPDMPVHVQNLKLDDFDIIKPLGKGAYSEVVLVKNKLTGHESALKIVDKNFLLKEKKMHHAFIEREVLSYLRHKGIIKLFNSFEENDKLYYSLEVLTDGNLLEYMNRHILNDSIIKFYTAEMLLILEYLHQNGLVHRDVKPENILLTKDKHLKLIDFGTLFVYDHNKIDIGNKLEDIRIQYQKQRSPSLNDFEKPQIKYCRDSSFVGTSEYLCPELIDYNVIGPQADLWALGCFIYQLYTNKTPFYSDNEFELFNNISQCSWSQDQRIPNDALDLIKILLDKDPIKRFLQEFDDSQYNYDSIKQHQFFRGINFKQMWLQDVPFINTINTHKRKMSRIQTTFIENNQRDKCDIRGISVTKNKADQIIIQGQLDKEHGVLFMTQFSLRHASIVCQDGIANFIYINPQLNNKKTIIPLNQFTSCKLLGKGKFIVGDRNKKKYIFKQRDNQVPAQQWVNIINKYIKSNHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5D8</accession>
    <name>A0A8S1N5D8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5D8"/>
    <dbReference type="OMA" id="TKDEHAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="50"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="283"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="532"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="866" mass="102787" checksum="1200C02DDD611617" modified="2022-10-12" version="1">MNILQQKQSQDVQETICKSHNLELIAVDLDMSDKSQINFFCGKCLVDKLNNNKVTTIEQSKERIQQIKNQQQEIKSKENQARLNYYKNILDQIMEFKRCIDDSLEKISKQIQQYMFPIQKEKKELQENLNQLNYFEDIKQLSELYSIDQQKSIKLIEDNIFIDELIRQFELLFNNAEYYQTLDIFKNTKQTIQDIMENNIIELPPLLQTKNEFVNNYIYKLKQKTPSLSRICQNHKKEIIMIDMDTENKKIEDRLVCVDCISENPLIKYQTIENVNKLWIDYNSQSENILQEYKKEIKHKKNELFNQISQMRKNYNLKLNEISDKLIQEQFQSISKPKQSNQIKTISIQTLQDEQLLKDLKQLIEKEKVNSNQTITILKNKDLIFQKEIENHLESLKQYDQLDIQQSIDILKEIPIERNVIVQLQEVISQISKILSTNDNNYQDQVEFIKNIQEFIDQAKKYQCQINLFDQTITLFQQHTNNIDQIQSKIQNQILNKQEQIQIQYQNLSKLLNDYIITFDNKTKQIKKYCNISKLEMDLIKLNELNSNLEIQKTNIQNQMSKQLDEKHQQFQQLLKEQQDKYLKQIDDINLKLQSNEKLNINLKDQIDLKTQEIINLKNQNEQDKLNIIKTIEDQKNKEIIEINKRLNQKESELQIIQKQFDQINQEKLQKEKQLKLDLEKVQYNYNTLLIQQIQQYPKTLQFSVTYKGQNFQVTEGGKVVEQTGGSYYYCLCEQAIPKTGKILFAFQILSLTSSMYIGIGFRDIIQKNNYQSAGVGYGSYMISQDGYTYSHHNKDVNGKYLSFTYTTNDIIIMEVSIEHKYIKWTRSNNPLATVQMEIDTSISQELYPCVLIYQSKIKILDNIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N758</accession>
    <name>A0A8S1N758_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0800221</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N758"/>
    <dbReference type="OMA" id="FECLPFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8088370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="31728" checksum="729F932A9DD3F50B" modified="2022-10-12" version="1">MAEFHCRYESHLGQAILGFCVNIDCQQHPQFCLKCLKDQQNNHSSQCLGFDMITENLSKYIQQEKLFYEQLQEIDKTFQMLLSQSLKKIQKNLKKLQNLNETLQKREYLKFKTEINFFRGYYSKVEETEKSEFLTSLNSIVLTLKTTTKGYVKIEDQQIAQTGDNHSQYLSRAKGRFDQGEKLFRERKYQEALTAFNDSLLMGYNHDFVYNWKAITLNHLNKYDEAIKCFDLALQINSTYESAYNNKGSQYYSQSFYRSFIKQFRQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NB34</accession>
    <name>A0A8S1NB34_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0810052</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NB34"/>
    <dbReference type="OMA" id="NQIQMLA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="254"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="327"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="564"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="87"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="656" mass="77662" checksum="3BCE708805819E3F" modified="2022-10-12" version="1">MNQKKGKAIPMQTLSFTITNQLPQIQTQSLTQVNNQIQTVQSAKQPPKPQKMFSDYMKQLMQNPETKKIYDDANNKVATPLIEDQKQQQQQQQQQQSKPFSIKQSQTSNSFTSNGNSTKKPFNQFYEQSKVRTINESIPRPQSEKRQQDNHSYVSTQKSEEISNEIDNKSVKSETFSISKSSQDLPDSNSKYVNKKRQEIKFDSDIWVKNEAPKYKLQNKEYKDIMNQVEQMVESRELSRQKQLKEFEGFIDSRQTSRTQNKQQINEEQELENQIQMLAIELDQVEKIELEQKTKIDIQRFQQLLDKPLENDSQFSQLSLKQRSMFITELLRNREQFKAKITGQQSESESPIKFQKLDNQEQYIEQIEKKIEKQQENLEKIAKCKLPNEYQLRLDSLLLDIDNFKQNSIKNYSNPFQIPKEKLIEIHKKIKKLNPKFVEDADLEFDKNCMLPKDPILRKEAIDRILEIKTQEVNSEILQLVSKGNSLEERPKMQQLIEQIKKKTSYTPSEFLKQKMKQFDELSKNSSNCAQELTTIQCNSMLNEFSEYKKQLDIAIKQNYDLLNQSKEQDLSNELEVLHEYQQKVQNLQNEYQQVQDFIEKFTLDYSLPTIKEEDEQNEDIKIQIEQQTQPLQSIEELDQEITRKVREQHPELFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEL5</accession>
    <name>A0A8S1NEL5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0860097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEL5"/>
    <dbReference type="OMA" id="MQNTDFV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8090562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="158" mass="18800" checksum="11F0CFF40C395467" modified="2022-10-12" version="1">MNNPFQSPIGNPNDPNNQLLLMSLAQVDSQNMFFFLMQNTDFVEKQDENQFKKTAIGFVRNTLGIFFLGTVLNLQMKRIPKFLNWPIYVKIPLRIPVFFSPFFLFQQSLTNQLDNLTQLHHKYYTRILRVKKTGDLRYFDPQGILQKQFEQKMKELMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEQ0</accession>
    <name>A0A8S1NEQ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0870149</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEQ0"/>
    <dbReference type="OMA" id="FILNKNH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="14"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8091138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="553" mass="64579" checksum="C448677F311CF7E1" modified="2022-10-12" version="1">MNNQQRIKVSVYSYDPDDLIGSGYKSQVFKGRNDMNGEIVAIKVCDHSKMQHEIERQLLQQEITALQVLDSYNIVKMHCYCQNTNFTYIITEYCNQGDLADLIIKRGYIPENEAVLIFYQILTGFKEQISKGVIHRDLKPSNILISKGIYKIADYGFSKMVYSPKEKVYYNVGTTLYMPPQALTENKYSEKSDVWSLGVMFYQILSGKVPWQAGTEQEFVKVITTTPIQFSRNIKISSTAKDLIIKCLQLEENKRYNCNQLLEHDLFRCFQVRPSRKSVQFSLNNSQLDNLENRAKTSQSQQRTNLQVQRLQSSKNLPETRQELVNYGEAVQNILNLLKLILRIAKIVDHFDFFIEGGLKEKLLYFTIKHAIFKSKQLIFILNKNHSTIDQTKKSEFLHQSNLMNDTFRISFNKLWDSIHKDETLLNEILQDKRFAAAFDQSDLEVHSHYILMLPLLRCCITYLQNQLNLKLINVREQDQLDGVEEGGILLLTYLVVYLEICMQVVSNYEKSIFYLNEFKLKLVVEEKPQNLTKRQFLQICQKIKSLQLSYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NF69</accession>
    <name>A0A8S1NF69_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NF69"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="128"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="346"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="443" mass="53095" checksum="8352E104992F755B" modified="2022-10-12" version="1">MLNTIFSLQVFRNLDIKAMIQAYDYALQNINELSNQIEQLEKSAIDIIVEKDQIIEQLELQLLHSSKRTSFNNQEEVVQYKNHIQELQIRLEHLQNKHFEQLQEQEKMWNQYLLDQIAQNEYTIDQKLKSHLEQIFILEDKINKLENELEIKENMQISLINQNKIFKENNTKLEEVQMTLNKQLLKYQIDIQELQQNYFKEKNQYLEQINQVNQELQELQSQSSTFKIQNQKLNNQNKIYTNTISDLMVKKAELELLIQTQKQDSIGIDKIEAFYSIDHQGENYHIRDEINTTAFDINEDTSDIKQELASFQFDENNVNFNHLKKSHSIYSFHSEKKENIDLDIHLKNSIQIIKQLNAQVQLLTQQIKVLKRQNINQNNAKEYFKIMEQNYSNSVQLKQQQIQTLEEKLAIQNQEILYLKQKVKQYTQKLIKYNKRLINNQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFI8</accession>
    <name>A0A8S1NFI8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0790155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFI8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="60" mass="6940" checksum="C7EDBD6F525BE176" modified="2022-10-12" version="1">MKFNIQYTSINDPNTFTLIIKYSLKVPGILGILITTVKKQKLQQQIQLHNQNPQQENVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NIV6</accession>
    <name>A0A8S1NIV6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0870212</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NIV6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8091259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="95" mass="11177" checksum="C49F01C5FC4BD182" modified="2022-10-12" version="1">MICEYERIKFIFPRFEKFIIMDLNTKIALEDLKFTFTNVLKIDLFARKLVFFITKGFNSIELDSQKSLQGQATLSNFKKDKTQIEIVVKLFEEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJE9</accession>
    <name>A0A8S1NJE9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0930020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJE9"/>
    <dbReference type="OMA" id="FIHIIPR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051884">
        <property type="entry name" value="Bis(5'-adenosyl)-TPase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46243">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46243:SF1">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="2"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8093337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="169" mass="19644" checksum="0A9E8DEBD93A7E7F" modified="2022-10-12" version="1">MDYFKFGQVIIPGNLVFWNKQYCYCIIPVVKLLPGHVLLIPKRQALRLQDLDPAEIFDLGLSVKFLTKNLEKYFDCTSSTVNISSFSNESDGLNHCFIHIIPRKEGDIKKNDDLYGLLENYPNDFIRQFHNTLGLGNAFSDQMRDTLSQEAKKYKEFLQQCLQEEIKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJM1</accession>
    <name>A0A8S1NJM1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0880136</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJM1"/>
    <dbReference type="OMA" id="QHCKEMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021622">
        <property type="entry name" value="Afadin/alpha-actinin-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47057">
        <property type="entry name" value="AFADIN/ALPHA-ACTININ-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47057:SF1">
        <property type="entry name" value="AFADIN_ALPHA-ACTININ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11559">
        <property type="entry name" value="ADIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="99"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="419"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8091569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="491" mass="58961" checksum="1CCDDDE30F363AC8" modified="2022-10-12" version="1">MDNYKKYLFDEKFQEPQPRNTYSFQAQESQEEQQIESNFDDEYKRDFLSNQIKNVKEKLIMMGYPPLGDLYSNNNSEVDLTIKLLVAIIKQRREDMDFKNSYHEKMSKIEAEKQQLSQNLERVTNSRKILEQENANLQAKIKSAEKVQKEQINKLMAEKEDYQKQLSKLQSRSTQFEHELKQRDLEIQKIKEHIKKQQSSKTYKNSVEITQTIEQGGPSLFAANGEYEFSQLVMKKWEEVNNKLLRENEQLRENLIKIHNELGEILQIRREVYIKRRKIDFGDENIPQELDNPLQNMHQFKTDLYKAPLETNGKQAIQMLTENLQTFKELMKKFDQQYELQMNEDDIEIEGEGGKIKACKNLTELFKNYNYIFDAQDKMISQIINKSQNLKKIDELNFNLNRFNRVLDDKQIDDVKKYLQDQKKYLDESKNEMDMIKKQFQQQLKKREEEKQIIQMKKQMLEETNEKFKENIRLLENASRQALQQLNQEYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NT67</accession>
    <name>A0A8S1NT67_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">guanylate cyclase</fullName>
            <ecNumber evidence="4">4.6.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Guanylyl cyclase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0990177</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">In the C-terminal section; belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">In the N-terminal section; belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YLQTRIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000026">
        <property type="entry name" value="Guanylyl cyclase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000045">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="308"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1033"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1065"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1112"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1153"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1179"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1221"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1350"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1375"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1403"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1427"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1453"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1492"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1897"/>
            <end position="1918"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1924"/>
            <end position="1944"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1956"/>
            <end position="1974"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1980"/>
            <end position="1996"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2003"/>
            <end position="2022"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2028"/>
            <end position="2049"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="16">
        <location>
            <begin position="1567"/>
            <end position="1702"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="16">
        <location>
            <begin position="2105"/>
            <end position="2236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8095838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2305" mass="266410" checksum="54F838FA27613BB1" modified="2022-10-12" version="1">MNYNHHQIVDRWLRRFRGCLNFCYGSDSQMITMELHKGGVQLLSVPQNKNIFNTRKYRLYNFLPKTLYSSLERFGNIYLLGISLIMLIDPTLSPFYRWITIFPVGLSVIFYVLMEFILDIRRQSHDHKINMQTTSRGAKDGSLETIKWSDIQIGDVLYLIKGDIVPADIILLDTGQVRDREAICMVDTQYYDGKSTLTKKKSSYLTQLIVLRTRLKNQFPEYRKMLTGKLEYEAPNGNTEKFHGRLKLKKDPKNEELTIDNFIPKGTKIKQTSWLFGLVVYVGENTKTMQSSHYNAQKHSFEEKQCNIYSFLMACLSLFFTLISIIVLLARSDEGNFALLIDENTTNGMKVFQLAILYAQLIPSTLYLLLDFVNFISLFKYEINQIEDNIIKYVKINTSNNLSDLGHVDYMLIDKTGTLTTSYYKLDNLLFGSLSFTLNYDQLQTTLNLKSAKPEDNEDPIKFASINDNNEYLIPFEYEKKSSPTDVQPSGKLLLKSVKTSNNNPVFSQITAIPQNKRMTMLNMLENSVQQPLPDYLQTRIFLPQPKTRNSPGNNNDEFIQLVNQLRNSSPQKEYLQTEDINTLYYDAFLKCLMLCHEARPVFGADSITYESFSKNEEISLTFARSCGYSLENFNKFDSPDMYLCKVRGNPIWYQVLGLNLFTYTRNLNSVVIQAPMAMDLELKQKYEAINQLCGEGSKNNSLLICKGDYEAIKTKLQLNHKEREELDSYIQHYKQRGIRMIIYATRVLSEKETENYKQQFNLLHSSLTNQDILLEKLALEYEKELNILGMIGFKEELKNDALDFIKTAKDCNINIWLLSGDQEVQTISCAQSLEMTETSKYMRIVATDKEQIWLQINTAIGQIQSELQKIQEKQQDKHLSENGMSRSMIKSSVTLFEGASYQQVLQFVMVVNGHSLSLISESPDLMSHFRFLSCVCKNVIGFNMNPQQKELACMIIRNYFPNNPTVLGVGDGYNDALMMQAANVSIEIINSKRNHIYPQVNAGDISVNTLSEIKVLLLQKCKLHSERVSSMIIYLFYCAGFLGMTLFFFNWFCQFTATSLHDSMTVFLYIFLYTTPNALVIGLADKQSQPFVNDKFPAFYIDGQIRTRRFWFYYLIEGFLESFICAAFTFYTCTYMVNYAWTNDAYQSDMQMVATSIIYLLITVSSFKVLFRLIQNNVNIVVIAFLLTFGLLVGFVFVNYRGDFSNFDYQELTYQLFTRFNSIVAIVFSLIGCYFINYFLHDFIKLIYFPNAYQQFAFQNKSGVEQEVMTNKEILHQCLDQHVNVSSIIQKVFIDCSATSPYIQEMLNPGDTKVTEMKLKPLTLEMKELVLEQKFLAHKLIQSLKHLRLFLCILLLYYIGYCLTDFFLTDKRVFTGAYYLVVFGIISIIILFSCTHRMEQQYYTYSHIIVFIIFAIKVAIDWLSDDLTITLSATIVILFSTFNSMNMSVIPIILYNICYLIQLIVRILIVVTSTDLSTSNGIYSQSRVSVYAASTQILLVVSITIRFLFSYYKSIKHRRNDYLAKYSIEQDNVAAQDILSILVPRFVRQQISTGIYSMQQAQDDVSILFAYICDFDTIMKEEGKNVVLMLDSLFRLYDNLCIQHGVQKIETVGYTYMAATGIKACEQNMTAHVTRIEKTMRLVNMAFDMMQQVQGRKYGKGNQIEMKIGIHVGRVIAGVIGHHKPQFSLIGDPVNQTSRVGSTGDTGAITLSEQAFKQARHGIKYYQKKQKEAKGLGIIDTYQVFKTKPIGYQIPRAFQLWQNCAKVVVKDLRQHRSSRQIQTKKQGQFLTQLHNSIYQDNLKQSNRIDVSPKGPQTVQFLIPQGSAQEENRSRLTIPAQPPEQILTEDSILITNEQEEKELELIKPNIILDIPENEIKSNFYQILKEQNVDESRVGMIFLWVTYFVITLLSIIVRKLFNHDLLIFVLRAIFLILAFVLFPILSKAYRNRAVNTMYYIVLIYAIFTVLFQAYLTDNREVAIICLLEILYIMIVTCQMKMFSFLQVILYMIIMFALFLGFYISTDLITHYAIFYICCCMLILLLGYYLAMSEQIQMFNNLQINEEKKVKQINLVSQLLPMHSYLKMKNSSIYDKSDFIDEFDDVTLLFADIKGFTEYSHTQTPEGVVTMLRNLFTEFDKLCQRYNVYKMYTIGDCYVVMGFTNSAKRNPIQEAINTVKMGFQMVDIIMSVRQKIKFDKLNMRIGIHTGQVTGGIIGTDIVRYDIYGKDVSIANKMESSGVEGRVQISETTKLMIERAGKHAFNFKFHHDVELNKFNMNIKGFLVDWDKRREEASIDQYKSPSRHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NV97</accession>
    <name>A0A8S1NV97_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0870102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NV97"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000093">
        <property type="entry name" value="NLI interacting factor-like phosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="52"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8091044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="327" mass="38241" checksum="527DE412004AD9A7" modified="2022-10-12" version="1">MQQKILYQKEQITKTKSIIARICSCLSFLKLKQNQTKQEKYQLGIDSPKSSFLGQRKLIVLDLDETLVHSQFQLTNGYDFAIDIIVQRQIFTVYVTIRPGVQQFIEQLNQYYDIVFWTASLQEYADPVMDFIDPNHIAIGRLYRDSCTPLQIGLTKNLNKLGRDLKEIIIIDNSEVSYHLNPENGFQIKDFYFDKQDQELELNIPFFIWLSQLPDVRPVAQLFKQFNNTQFDVFKMKNTTILGTQYQENDTKIIQSLSKSLSLQKARIMHCNQVFRTLTLSKEDDEDELDEIKIRDKLGLNQPTGEKGIQQQNQTDEDDKDTYVMAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYW8</accession>
    <name>A0A8S1NYW8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anaphase-promoting complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0970135</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYW8"/>
    <dbReference type="OMA" id="CVIQAKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037679">
        <property type="entry name" value="Apc5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830:SF9">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035904294" description="Anaphase-promoting complex subunit 5" evidence="5">
        <location>
            <begin position="19"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8094993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="627" mass="74072" checksum="D6D93FD9BD1560E4" modified="2022-10-12" version="1">MNNNLILLILLLRKPSQQVYDLLTEYLKIHDVTEIINNPTLQNTILSHIEPELLQITSMNKFHKIIGETLREKQQQKTPGGSVVDLFFRNMLLLFNQMDFNQMLKSWSDFKEGQDNHLQNNPFLTDSYIEFLLTKLQTSSSYVNLEELKQIIEQPNLNENCKVNFLKALLYAILRYPFRAIKFSRKYYDMTLNSLVPKKVNHCIMNSIYLNMKMNFYDEALNSLSEGLRLSQTVSDEQSINLCLLNLFEIAYRNNYQKQATLLLEYAVNHAQQLSPQHRLQSALIYGSQVRYKQINYSLLKKKHINWKDIIHQSLKQILQSHSQDIEQSITAYQLVCANNYGNSTMIQNALDQLQQFDQNDQTLSLQLEALSQISLHQPLYTLSQFQQCFETTYNLSEQSILVILNICLEYFEIINEPISIKYIKQIMMQLLQMNPDPYISESLQIVPIKNPKLRVKQLLKDGENDLEIIRLCKQFHLNQQYLEYKLNIIERNIKQNQIQIAQDKLNNINLYELSQSNCVIQAKFWNLMARIYKSLQYYATSINFAIQVGWISMIQQNYYDMTFIYDQLDQYDQRDYCAEQFNKVDALISQCIQNTNLIFFKINEIGIVEYLYRFLNNIQQQFSCNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P2C8</accession>
    <name>A0A8S1P2C8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0990056</name>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T1310051</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P2C8"/>
    <dbReference type="OMA" id="YKGAIWL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000005">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="9"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="106"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="209"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8095604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8106562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="313" mass="33923" checksum="B92EF5CD044D44A1" modified="2022-10-12" version="1">MGGDKHDLSYYVKCMIGGTLACGLTHTAIVPLDVVKCRRQVFPTLYKSLGDGLSTIYKTEGFGGLALAWGPTLIGYSLQGLGKFGFYEIFKDVYKSVVGEENANKYRRIGWSIASGSAEIIADTLLCPMEAIKVRMQTSKPGTFTTSGSQAFNQVKSNEGINGLYKGLGPLWARQVPYTIVKFVAFEQIVALFYENVFTKPKDSYSKFTQLSVTFASGYLAGIFCAVVSHPADTIVSKLNSIQTGGSLGENVGKIYKEIGFSGLWRGLGTRIIMIGTLTGLQWWIYDSFKTAVGLQTTGGGSSSAPKPQETKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P380</accession>
    <name>A0A8S1P380_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1040053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P380"/>
    <dbReference type="OMA" id="FEDASPY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="485" mass="56896" checksum="3E6A1FBFFBDD2E9F" modified="2022-10-12" version="1">MIVKQKREVFHVQLRRNQREQIFSKKRILDQPQQQEAVTSEQAILFLKGGMSNQINLAEMFQELHQLLPKYGDLHLKTLPYIIQGVKKYLQDADSSLLYMNDEDREISEYTVLEILQYLGSLTYHIKVEFDLDNLIEMLVAILNSTDEFKMIERLCQIFQNLLFDFEDASPYLLKCEIVTKLANKFLDCNVSYDLNNLAPMFRVLQQLDGVNYYDFNQIHPLLHRLQNDSKVDTQIYCEIIEGFFSFLVKCTNQLTIQDDSQKLLQFQLFNKLFEFQYVNWSVNLLYKYKNQKKLVPVIYSFLHNLSLGDCGKKLISQGVLTAIFEHLSDKIVSQNQLYSILTNLIVDDFDSVMKSGILKKVHSQFEQGFYSADYINELMYCITNAFYTASESQILELIKLRLGDCLILYISQQPMELLDLELCSAIFQAIHSVFVKQPQKSVYSLKKKIRNDSIFLARSSQIYDISHKDDKEYEDMLKYLNKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P4E0</accession>
    <name>A0A8S1P4E0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA topoisomerase I eukaryotic-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1050072</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type IB topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P4E0"/>
    <dbReference type="OMA" id="GECPVTT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00659">
        <property type="entry name" value="Topo_IB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.10:FF:000011">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025834">
        <property type="entry name" value="TopoI_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013500">
        <property type="entry name" value="TopoI_cat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008336">
        <property type="entry name" value="TopoI_DNA-bd_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013499">
        <property type="entry name" value="TopoI_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051062">
        <property type="entry name" value="Topoisomerase_IB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290:SF3">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290">
        <property type="entry name" value="DNA TOPOISOMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14370">
        <property type="entry name" value="Topo_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01028">
        <property type="entry name" value="Topoisom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02919">
        <property type="entry name" value="Topoisom_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00435">
        <property type="entry name" value="TOPEUc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52038">
        <property type="entry name" value="TOPO_IB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="DNA topoisomerase I eukaryotic-type" evidence="3">
        <location>
            <begin position="180"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="520"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="1">
        <location>
            <position position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8097860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="596" mass="70318" checksum="7CEAFF4198345F73" modified="2022-10-12" version="1">MGDKKIKIDEYKEIRLGGFQARSHNWWEEANPEESEEEEQQGQEWRYLEHHGVLFPPFYKRHNVGLIYNGKLITLTEEQEELCTYWAQSIGSPYENKEIYRKNFESLMQTKFEGFQLDKADFTKIKEYLEQQRLLRLNKSDEQKKQEKLERDQRELFYGYALVDGCLERVGNYTLEQPTLFKGRGEHPKAGLLKARIFPEELTVNLSTQAPVPQCKLPGHSWGKIVHRNDVTWLFSYVDSSIRKDNHKYVQFAATSRFKQMNDKRKYEKARRLKNCIDQIRENYTKKLQSDSIKERQLGTATYLIDKLALRVGNEKDEDEADTVGCCSLRVEHIKLKEDDVVAFDFLGKDSMRYNNEVKLAHQIWKNLKLFIKGKQPEDNLFSDISVDDLNDFLKKQMEGLTAKVFRTYNASFTLQQQLDLGGTLDGHSIQQKVDFYDNANKQVAILCNHQKTISKQFELTKKKINTKLECMKTYIHELEQHYKKKNPNEYEEEVQLEEGSQKKNSIFIKKFPGQHDKIKQQIQRTQEKYQKELKGLEKKEENKEIALGTSKTNYNDPRISVAFCKVHDVPIERVFSKTLRNKFIWAMATDSNWKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6K7</accession>
    <name>A0A8S1P6K7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1070171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6K7"/>
    <dbReference type="OMA" id="MLRICHA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8098699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="490" mass="57860" checksum="F8BD2A216845E6D4" modified="2022-10-12" version="1">MIKYCSRLMRICYRIPIYRFCNEKFQISEEDIEKLKQLYQKKENFEQFLQQKEQILENVFKEREQLISFKLMQMSDVDEVFEFYSKEIAKQLDEKNPQPPFNCNELSLFLYFTSCLRNEDYADQMKNHSDRFQQITSKILLEINRLDPEQFCAFVWALSTYQIEMGDLNLNDSKQFIIKQQAEIILEKIPPETLPSLAFSLYHILKDKNDLILRISKLTQQNISIQTPSNLITLIKSLPIGSFTNSKIIFEQLSDQFQQDLLSQDQFVNVLVELLRITNHENTEYVKEMVDLLTQKIESTQKHNLNISMQKVIDLILSCLELEQQEGEQLVKQMLRICHAKSEKLNISEYIQLFLAVAKIKNKNYESINLEKTIKILIELSHRSQVFTLNDFEQKELVQVIVASAAIRIDNKDFIRNIITKIDPLLLHKQDLLLLAKSFVIYVRLFEVELLKIHSICAKKKYEFSVLEQAQLTQTFNRIKVLIPDSPFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8E9</accession>
    <name>A0A8S1P8E9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1090097</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF-assemblin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8E9"/>
    <dbReference type="OMA" id="MFKEMIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008374">
        <property type="entry name" value="SF_assemblin/giardin_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06705">
        <property type="entry name" value="SF-assemblin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8099339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="247" mass="29061" checksum="05064F7C0C0A4847" modified="2022-10-12" version="1">MSRPYSPTRSRAEKIVGQLSEITLGHKDQTSRIKEAEERVEILETAVKDLKETLEGKISKIKEGLEYVQQVLEEQEPKQEDEQQMIEDMKKAEESVNEKLEELSKQREESQQKFNEQVEKDIQELKEQISQEKSERNQSIKEINECLESDLPQLQEMLQQNIKERDEMDKNINTRLTEELQRLSQIIQQEKVSRQQGEQSLFEMLKDVVNRIKKEIDEEKVQREATENDVLGVLEETLNKILAAQDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCF8</accession>
    <name>A0A8S1PCF8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1130114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCF8"/>
    <dbReference type="OMA" id="CRRIPHF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="277" mass="32862" checksum="5EC7249DE4160A75" modified="2022-10-12" version="1">MKTTNEPSLLKYRIIEQKNKKIVLEADEDQFTQFANALSIIQKFHDDIKSQFHPSSQPQRRTKNVVSIQAINSDSEKSNNQSFANPNPDIKEDQDFRRRIPHFFLSRFKKWAKMMEQDAAYKYLQNVQESKTSKQQRFELGDLQRCFQVQVNEPKESKLCKNLLKDLFISFLQNEATLQIIHYNKISSIEQKHKYISEIKNMIQEMYELKPFDSYLSSEKIKGKRNAQNNNNQSPIKEKQEDLREEFQIPEMHEKYSNESFKKYGNGPSLVKMNSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PF62</accession>
    <name>A0A8S1PF62_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pyruvate dehydrogenase E1 component subunit beta</fullName>
            <ecNumber evidence="4">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1160045</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PF62"/>
    <dbReference type="OMA" id="WYANCPG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027110">
        <property type="entry name" value="PDHB_mito-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006667">
        <property type="entry name" value="PRK09212.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008854">
        <property type="entry name" value="PRK11892.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624">
        <property type="entry name" value="DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624:SF96">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="5">
        <location>
            <begin position="15"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8101614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="340" mass="37517" checksum="B762C8F5C38265F5" modified="2022-10-12" version="1">MLRIVHKLRSGFAKMTVREAINSAMAQEIERDPNVFLIGEEVGQYQGAYKVSKGLYDRFGKSRIWDTPITEAGFTGLSVGASMYGLKPIVEFMTFNFAMQAIDHIINSAAKLHYMSAGGLKTSIVFRGINGAAASVAAQHSQCFAAWYSQVPGLIVLSPYDCEDARGLLKAAVRDPNPVVFLENEIMYNEAFEVPDNVMDKDYLIPIGKAKIMKEGKDVTIVTFSKMVKFSMLAAAELEREGISCEVINLRTLKPLDRNTILESIKKTHRVVTVEEGWGQCGIGAEICSIINESNAFFHLDAPVVRVTGADIPTPYAFNLEELSFPKAHNIVEAVKLVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGR0</accession>
    <name>A0A8S1PGR0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1180034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGR0"/>
    <dbReference type="OMA" id="DKPFHRN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="221" mass="26166" checksum="2B0B83505B4F6001" modified="2022-10-12" version="1">MANQSQPPPIQEKTFHEWSKNNFYRTSYINHYTQFPQEPKNTAVPGYAGYVPYVQSENLYGERFSEVARKSFSDQKLGKFNRLSSTGFNFDPKELIDVHKEAYSHKYGCQTLLKNHPCTHINKMITSYQDGFKKPQELVAPTFRKTERYLETSQAQTKTSGFQKNHMQFDGSGWIPHENMNGDQVRTEYRIQYNQDKPFHRNPIQFKLRKMKQTEMNYKHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGW9</accession>
    <name>A0A8S1PGW9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CDT1 Geminin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1170163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGW9"/>
    <dbReference type="OMA" id="KYPEWIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="41084" checksum="2757B5D4AD381D3C" modified="2022-10-12" version="1">MSYSRNGVSQHKNKDNSQHITKRDKKEAKVKGNVQQLVQKSMTKNKSLYEDSLTINSIEQFENYKKMVKLEQKRESTIDISIILFKEPLRQRFERIQSIKTIQQLLAIPYYQLFCKMFLNLNKILINQTLAFQEIVIQFKQDYQIEFKVQYLQQFLSIWNDSFSLAWKHHMNNKLSLEVRINNQQFLQNDNKNIIFQQKLLNYIQTQGECIELFPLPNIENTQQQDIKLQEQESQMTQNICRDQQDYQKNNQLRSIAIQLQHYFHIRDVSNMYLINVEKYLIKQKFDLNFNNGLQKIINELILKYPEWINMIQGEKEQIIRINRSINIKQLMLEQECLDCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHA1</accession>
    <name>A0A8S1PHA1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1170190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHA1"/>
    <dbReference type="OMA" id="NINSGME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051266">
        <property type="entry name" value="CLCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579">
        <property type="entry name" value="CALCIUM-ACTIVATED CHLORIDE CHANNEL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579:SF43">
        <property type="entry name" value="ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="173"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="595" mass="68624" checksum="CDA147E3EF1C5380" modified="2022-10-12" version="1">MFQTNFQQQPRKIYVLQRTQSQLPMNLQQQPQEQKLEYFQQSKIAHQPVMQTLQYQMNPHINQLKFKQGESVQQTQLGQQIVYQPNLQNIQRSIVAQKIINYQDDELIQPKKENAKQTAKYDLIENLQFEIKTLYKMVKLFPTKAQYLPGIVSIKAKDQTITQNHENQRVGIDLICLIDISGSMIGVKIQMVKQSLIVLLQFLGDNDRLQLITFDDDAHRLTPLKRVTKQNKIYFTQIIQQIQANGGNQISEATKMAFCQLRHRKYINNVTSIFLLSDGVDYTFPEIKEQIKTVNEVFTLHTFGFGQDHDAPMMTQICNLKSGSFYFVQDVTLLDEFFADALGGLISVVGEQLEITITSQAPPPYQNIQISKTYGNMWQKKGNQYYITQPQFASGSRKDYVFEIALPKFQGRIQDYQRSIGVLFAELKIQDPINRSIIKIKARLDLNLFNQDEQINENIYPDIDVYAQYFRVKGTEVIDEARKACEQNKNEDAEKLIDNMLIQIQKIPNVAAQCTGIIQDLQQAKQASARTSYSLFGQKQMCQMVSNNYQQGGVNSLFSAQGMQLQQVQPAAYQNVQQQQMMVQVQKSKIPQKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMW6</accession>
    <name>A0A8S1PMW6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1230130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMW6"/>
    <dbReference type="OMA" id="YSRNHQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="231" mass="27349" checksum="CC3873FD5F679CB8" modified="2022-10-12" version="1">MMIRSFHSISFNQNSYIPSQNQIRRNSCHCSHCSSSNIKQITLPNQLYEIVMHRKIRLANLLINTFKPTDKYLRSKQKYKNFHIYSRNHQQFNSPFSHNSPQKTSTPSSQDEIKDNSQNKKISSIYSQYQHSKKSNTYFLSQLKTQMTRIDKNLNQLIQTSRQQITEPDELNIHQKRTKQTIIKTIKTHHPYKSEYSITLNKYLLTPKKIYTKPKNLHAKTQQSLSKFAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTJ3</accession>
    <name>A0A8S1PTJ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1310110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTJ3"/>
    <dbReference type="OMA" id="LSYAKIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000220">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="90"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="237"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8106680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="604" mass="71401" checksum="34DBEA617B1D10A2" modified="2022-10-12" version="1">MKNSTTQKGVTNTFQQIVMSAKQKEDNERRNMSILEKAATLWQRDSSHTKTIQLQRTIYILKQYSQNRTDEQLEAVKTYFEENFPYYSKFKEKLDHESCKSLFREMTLEWYDSTKIIFRHGDPGRRMYFVLQGELVILIPKTKNTSENQPQKKQKFNSFDEWVKYVFSDYTQVATKIEGDQFGEIAIEQKVTRTATVAAKTEVILAVITYDLYQRILGAFQHELTNQKIAFITKIPIFSVWQRQNQLVLLQSLEVQNKKVGQFIFQKGKPDEYIYLVINGEIEVIQWSTPNKDQISNHHNFVPKPSIMAIINSGQFFGDYEHFKQLNYRITTAKAKTDSTYYRIKYRQLLDYFQIYSSIDDFLKYENVKFKIQSHLGQRTNQNLNLTEPTGDQTYQFNLDQIKSSYYQKIKVEQQIDKLRKKMEKTVEQAQKAIYYYEIENLKQSIENIETIQAEKYSFLVPFRSDPYVNEMNLIRSETRNQRNRLQLQSESDLRSKDIQFSLRSSIKTQEQTRSQSQSNFNSLSYAKIIQNKLKKLSINVQPSIEINKPQFTSSKTTKPENHPTNVESIHNVRMNFTPISNSQLFNKKKHLGNVYKFQIKSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PX25</accession>
    <name>A0A8S1PX25_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1330068</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PX25"/>
    <dbReference type="OMA" id="NIEGEQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="175" mass="20482" checksum="26EC5073701071FF" modified="2022-10-12" version="1">MIKQTKDGEPIFIPPAKILQELKYLHKELPAFCYSWAWEARCVCAFEKAEFIEAIKELKQIGCQKRCEVYAKKPFLIGKRLFNVRLISYGASSPKKMFFGPNHLAHAFDKLMFDYVYIFLETPQKTAKKLQLLIQKAESTNNNRQIASFKESYKILLGKEYEEQNIEGEQNEEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q0Y3</accession>
    <name>A0A8S1Q0Y3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Thymidine kinase</fullName>
            <ecNumber evidence="2 11">2.7.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T1370100</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>thymidine + ATP = dTMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19129"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17748"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the thymidine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.21" evidence="2 11"/>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q0Y3"/>
    <dbReference type="OMA" id="ATHSKMT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004797">
        <property type="term" value="F:thymidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046104">
        <property type="term" value="P:thymidine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001270">
        <property type="entry name" value="Thymidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001267">
        <property type="entry name" value="Thymidine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020633">
        <property type="entry name" value="Thymidine_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11441">
        <property type="entry name" value="THYMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11441:SF0">
        <property type="entry name" value="THYMIDINE KINASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00265">
        <property type="entry name" value="TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035805">
        <property type="entry name" value="TK_cell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00603">
        <property type="entry name" value="TK_CELLULAR_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8108518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="205" mass="23536" checksum="49720971B4296CA6" modified="2022-10-12" version="1">MYTGTISVIYGPMFSGKTSELMRLVKRFTISERKCVVLNYANDNRYSEEQCISSHDKQYLKAIKVCKLNEAYEKCKESDVVAIDEGQFFSDIVEFSEKMANLGKIVIVAALDGTFDRKPFHNILNLIPLAERITKLTAVCWFCKRENASFTKRIVQSQEIELIGGEDCYKPACRVCFHLAETQSKTQSILYVNQENHFEQKNLIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4W8</accession>
    <name>A0A8S1Q4W8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1430100</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000147">
        <property type="protein sequence ID" value="CAD8110267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q4W8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF26">
        <property type="entry name" value="VESICLE TRANSPORT THROUGH INTERACTION WITH T-SNARES HOMOLOG 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8110267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="13188" checksum="0A7C86CDDAAE38DE" modified="2022-10-12" version="1">MKPNTNNKAFNNGFRQNSSNSNVGLMIDQQNSLNYSHRLAEESLQIGEDVLESFKRQNDSLKRIQNKVNYTIGDLGLSQGIIKLIGSRDQQDRRIVIFLTILLFAFIGTLYYVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QB12</accession>
    <name>A0A8S1QB12_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1520044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QB12"/>
    <dbReference type="OMA" id="HEQPTRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="584"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8112593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="708" mass="83665" checksum="1224BB215810F18D" modified="2022-10-12" version="1">MGQQIIGKCKQCGPQNEDILPQMEFQKEIKSEVIKNLKQKLHEFESYSIQKLTTYSLKYDGLFRKEDIKKEKVIDSLGLVKPMDIEKIKQLNEEAGIKFYCGISLGDPINIGFKTINGSLIELNQNGYTLVRLWGMGEMMMNFQSCIQIEGIKLIGLVRGDIETYKKIIMNIGWEEYDHIIIEDDNILDLQYDEQTHQINHNSILSALLLYDGQVIYKHYIDKLQFQSDYHIVKKDVLEIIENGVKQVNQINVITNISFIKQQEYIKLKQFIMNQKKQSFSRDVEVTIKKKTIYYKEDKQVYYDQPIIYINSSPKSFSISDKFANQLGKKNLKWDSKLLKAKINEKIKEFSLNVQIALQKDIIISITKEYCEIKVHHWEDGILVPYYKSLDYISQIKLWITATETIRYEPNEDPVIDRINDLVAKLFQTIPKVDKVLGTKYANIPFVEKWKPPYEELDPHPKNLEIHRKSDKILLIWLFDYEIEENIEKLKELIKIKKNNPQFGLQIAILGYIKFTTWTSKFQAFLDKYELTRSILDNTIETWFPQEEQMGRFRFNIILQRIYGLMLDKQDMMIIDSKGIVRLIDNSTQIISNLTQEIDKLNEKVKVTVKNYNYWRQYQNLKKCIRENPQLAEIVKKIQEKGIVEESKVLIEQMKDKVWTFENGQIKEEKWHEQPTRILRNISDFTDINEMARIIAKYIDNNDICALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCG5</accession>
    <name>A0A8S1QCG5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1520043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCG5"/>
    <dbReference type="OMA" id="ATELFNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="194"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8112591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="374" mass="44715" checksum="B4C349C6D10B38AC" modified="2022-10-12" version="1">MCDSDSDVEILDFEQGLKLQQNTIENLEKELQIVITNSSYYQQQIQELLSEKNSMKKRVKELEEQNLQLENNLQKQNQTYQRTIEELNFESQKNEEKLRKHLESLMEQQKQEFERILEKQSEQINDYVKKLENQEEENNIIQNKLIATELFNCKLQSSLSELQMDIKTKAETRKCVQQFNEKLMGENINNLNKIESLSMQVDGLEEKLQKLTQQRDHFKKQFVDISQKILQYQQEISQSNSIKLDLKNKYEICSEQMKKYQQLLRDCADENKRLKQIIGQFQSQIEDSKYKSDLYIKENTNLKNQLNTVQQYYKEQINQLQLTLSSKDAQIQQLSEPNKLTIKRLQKQNQSILSIQQQEQEKRQLIEQKIKSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QD83</accession>
    <name>A0A8S1QD83_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1530067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QD83"/>
    <dbReference type="OMA" id="KYCSDID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="410" mass="48980" checksum="AD866E3448D087D1" modified="2022-10-12" version="1">MSLLYSKQTISKSDLIESLTLLLQPKSQQDFDFACQYFGSLMKKHTKEVFNILRQKQYVKFVEPKTENLLNILFVIHSNIEEESIANQLREPAIYWLQKLLKENKESFKQIVNEINTSGLLTHQQFIIKQNNIHNKIELITEIRQSSYDIQNHVIKHQVEEEDQKKSKLFCVIYYSYLQKLSINLLVYKSIKNLQYPFEENEDMKGKIVWIYKIQNIMQTGLKLLEYSNKQFKLYQDFIYQNMIQFLIFIRQEIEYLINQYAIISNLDCLSLYEIYIEFLKSQTIVEKYCSDIDLKIRLNTIQIEEFLNFSVRIKVLNQCAFKKSIKVPSKHQPQISCNLIIDEEGKHQKAQSEFNKENMDQLYLIDQISDETQITEKRYENKSRENEVHMEVDSNRQTHFKGLSTFQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDK8</accession>
    <name>A0A8S1QDK8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1540098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDK8"/>
    <dbReference type="OMA" id="WILVIQL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="23033" checksum="9602AAA30B7D0224" modified="2022-10-12" version="1">MNQILPNINSKTNALLQLIFLTTAGLLLCILLFSTYREEEIHQNGELLKCKLKYDAKMKCQLNNRTLNNNKITKEDCQLEYICNSILATYPITIIDGVLIIVTIVMSLMKHSNLPRNITTFNFVSFISILLSASTLFIMSIILMTGAQNENLSIWILVIQLLISLILFTSLIFIKKEQEFSNQIKKQEEMNGTEIHQYVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDZ0</accession>
    <name>A0A8S1QDZ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1570088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000162">
        <property type="protein sequence ID" value="CAD8113982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDZ0"/>
    <dbReference type="OMA" id="RISELXG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8113982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19365" checksum="9E7962C26079BA71" modified="2022-10-12" version="1">MGSCCSSNKIVKETYDYPDGGVYVGDMENNAPHGRGTIKWDNGSLFEGDFIDGRKNGRGLFRWNDGQYYDGNFKDEKFDGYGEYFWTDGKIYKGNWIDGEMSGQGEMIYPDGRVYKGSFKADKKDGDGEMTWPNGNKYIGPWKKGKQHGKGVMIDKKGNQTEGIWNKGKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF79</accession>
    <name>A0A8S1QF79_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1590030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000164">
        <property type="protein sequence ID" value="CAD8114272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF79"/>
    <dbReference type="OMA" id="FDIQIWD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="83" mass="9687" checksum="D1C26233F20A584C" modified="2022-10-12" version="1">MQNLTQSPLKHLIIEGDTSPINLIDDFELSSQSSQNNTFIQSGRKNSDGDNTVRRKKFRSQDFTEQLYMWNGYLVKKFESKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QHX8</accession>
    <name>A0A8S1QHX8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Opioid growth factor receptor (OGFr) conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1600097</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the opioid growth factor receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QHX8"/>
    <dbReference type="OMA" id="KERYHET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140625">
        <property type="term" value="F:opioid growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006757">
        <property type="entry name" value="OGF_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039574">
        <property type="entry name" value="OGFr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14015:SF2">
        <property type="entry name" value="OPIOID GROWTH FACTOR RECEPTOR (OGFR) CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14015">
        <property type="entry name" value="OPIOID GROWTH FACTOR RECEPTOR OGFR ZETA-TYPE OPIOID RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04664">
        <property type="entry name" value="OGFr_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Opioid growth factor receptor (OGFr) conserved" evidence="3">
        <location>
            <begin position="55"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8114611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="358" mass="43133" checksum="971883D256EE7BAD" modified="2022-10-12" version="1">MKQNKDAKITNQRSKSESNQNGLKKLKQPNYDFHKTRKDGVKKDILYHIEKWNQYQENSKEIKNFYKDLEQDHSFIQWIFPNFFNSMFNSDSYRLTIEERNQMIEDNDIMRFYISNYKMFLRFLGIKWNNEKLELENKNQFNICLQINTHNQLRLRRVLASLSVLGQRKLAIQLLDFIYKECNKLYGTQYYLYDLVQEEQQKYQIDFQSQQDYQDLQQIKQQEKIEKINIDQSAMKSKMIQDPPIKQQDNKQGIGGHSIKEIQQKQGFNKVEKEQQKGLEEIKLEDKTKSNFKNQNSLANKQFLIQKKPNYLHGKYDRAQLLFNFDEVKNNYVQGDLLDKTWVTINLLSNKKVDIQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QK24</accession>
    <name>A0A8S1QK24_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1650023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QK24"/>
    <dbReference type="OMA" id="CLNEVTP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8115534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="98" mass="11395" checksum="C5B2D1703E21CE75" modified="2022-10-12" version="1">MNDTIQFDDCLNEATSQEKDIINYLQDLKLIFLTDPQQKQFKFEQAKKQLIFSYRSLDSDTDTSEMTPPMLVKTSSLLLTKSPLRQKRLSIVSEHPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QN29</accession>
    <name>A0A8S1QN29_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1900008</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000199">
        <property type="protein sequence ID" value="CAD8117163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="276"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8117163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="730" mass="85144" checksum="C96ECA30054DA455" modified="2022-10-12" version="1">MMLDKYLNQDPQYINNDFSISDDSSLLVLATNRGIRLKNQETNKEIFYTLDQKYLLIRFIEQSQKFVAIAQSFLYLYKLDDINNPIVFQHLILCGNKIQAIYNLEQSNSMMILYDKFITIHELKEKGNNQLLEIQNEFSISKDCLILSKSNYVGIGIDNDIRFIPLSNSIKMHNIYNAFVSQFYNILYYSFSLNCQEFIIIGLNYEEKLQRAKINLINLEIEIKSSEINFGFNINIYSNQKQDKLLLVHKDNQNYQRKLDLIDFESLKIIQCIEQLNHNESSCNYAIFSEDNSYFITKYKDQTIKLWDSKTYKLLSKFKSDTTNFDLISISTKEILAQSNNHIIKLWNLKALRSQQSEMDGHSDSINLISISPDGLQLVSGSLTQIIRWELTKFKKIDVLIKGQGLRSIFCFSEDANYFAAVDKDPCLIIIWKLNTLYLIENFMTISYFTQAINLKFKIADNHLISCYQIESQLYHIAWDLSKPELYQIQRKTCYVDIPKPVMQFLSDNNLMIQIKPLKIFVFFDQENSEYFQFDNSQSISDAKLVTITHDNLKMAVQNESEIIIWSFKKQQIEHRFENDIGCTQNLVFSGDNKILFTNYNVTIRCLDVTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYTGRIKFNYNKSEFQRKSQAKNIWSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQ59</accession>
    <name>A0A8S1QQ59_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1750017</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000184">
        <property type="protein sequence ID" value="CAD8116590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="SHAYSIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1427"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1498"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1542"/>
            <end position="1563"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="303"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="5">
        <location>
            <begin position="317"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="345"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="387"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="429"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="471"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="5">
        <location>
            <begin position="479"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="513"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="555"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="597"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8116590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1592" mass="180248" checksum="A64C0D10FA88B3A6" modified="2022-10-12" version="1">MQGSFQVKEALGDILAQVKNVDEQIFGVLLKMFRKEKVQDCIGYLSDMGNQRKLESQILQQVRNFTQTDTEQILSDVRNDMKQITDVLRKLKDHDFNQKDFSSEKNEEFTFSLIKFIKNNRRIKGFLQFLVHLTSIDETFIQCGSNSFHLLVQMKVDLRNKNFVKIKIQKTSLEGANFFGCNFCESQFNDVNISGINLNEALLFNCQWKNLRIPELNKLFGHSDYVRSVCFSPDGNTLASGSRDKYICVWDVKTGQLKTKFYGHIHFVYSVCFSPDGNILASGSYDKSIHLWDVKTKKKIAKWNGHTDTVQSICFSPDGNTLASCSNDNSIRLWDVKTGQQKAKLDGHNNTVYSVCFSTDGNTLASGSEDNSIRLWDVKTRQQTAKLVGHNNTVYSVCFSPDGNTLASGSEDNSIRLWDVKIGQQKAKLDGHTGYVYSVCFSTDGNTLASSSGDKSIRLWDVKTGQLKCKLDGHNNTVYSVCFSPDRNTLASGSYDKSIRLWDVKTGQQIVKLDGHQYQVNSVCFSPDGNTLASGGNDKSIRLCDVKTGQEKAKLHGHSNIVYSVCFSPDGNTLASGSRDKSIRLWDVKIGQLKCKLDGHNNTVYSVCFSPDRNTLASGSYDKSIRLWDVKTGQQIVKLDGHXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVSLLMEIHQLLVVVISLSVYGMSKQDNKMPYQMVIVILSTQSVSLLMETNQHLVVEITPSIYGMSKQDNKKPNQMIILIMSTQSVSLLMEVHQLLVVEISLSVFGILRMEKKFHPPKKNIKIFLPSLNYHFRIAHYYQMLFIIQLTMIIQQLELVKILYQKHRVHLSYKENSQTIKEKIQNLYLNRKEVIFQKTYRKNDLNHYQAKLIISINIEYFFFNFVMISLTINLHFEIQVRSYFSKFFPFLFYIPYIYLNIAKIYCQFSVGIIYLNIKKNVINQYLQRLDYKLYYIKSTIQTEDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQU3</accession>
    <name>A0A8S1QQU3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1840003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000193">
        <property type="protein sequence ID" value="CAD8116920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQU3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="8929" checksum="367AAF126C9CA2DA" modified="2022-10-12" version="1">MIQNEEKILKFRYSMMEQQTQQQLQQNIVIGFIIQFIVTSLILLFQVQVIILLSDGNKQIQMIRLVQNVINTIHIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QRM1</accession>
    <name>A0A8S1QRM1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T2030001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000212">
        <property type="protein sequence ID" value="CAD8117535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NGHDDCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="217"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="259"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="301"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="318"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="343"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="385"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="427"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="469"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="483"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="511"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="553"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="595"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="637"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="678"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="720"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1323"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1365"/>
            <end position="1406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8117535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1485" mass="164437" checksum="C8D76CFF5DBD71C1" modified="2022-10-12" version="1">MQSPLQEKETLANIFYQIKDVNEEIFGVILEILRKEKVYDCIGYLSDNRNQQQFEQQILKQGDNLKPNNQEQKLSIVRNDMKQITDVLRKLKDHDFNNQDFSSKEQEESTLSFSIKNNRGIIVFLQFLVHLTSIDETLIQGGSNSLYLLIKMKVDLRNQNFENIKIQNTSLVGANFVRCNLSGSQFDNINLMGINLNGAQLFNCVWKNIKIHELNKLDGHTSDVNSICFSSDGNTLASGSNDNSIRLWDVKTGQQKAQLDGHSDYVTQVCFSPDGNTLASCSGDKSIILWDVNTRKKRGKLFGHTNQVNQVCFSSDGTILASCSNDKSICLWQITTGKLKAKLESHTSDVLSVCFSPDGNTLASGSQDYSIFLFDVKTGQQKAKFNGHNSCVNSVCFSPDGYTLASGSNDKSISLWDVKTGQQLAQLDGHEYSVNSICFSPDGNTLASGSNDQSIRLWDVKTGLQKAQFDGHSKYVKSIFFSPDGSTLASGSEDKSIRLWTAKTGLQKAKLDGHNSCVNSVCFSPDGYALASGSNDKSISLWDVKTGQQIAQLDGHEYSVNSVCFSPDGNTLASGSNDQSIRLWDVKTGLQKAQLNGHDDCINSVCFSPDGNTLASCSEDMSIRLWDVKTGQQKAQLNGHDDCINSVCFSPDGNTLASCSDDKSIRLWDVKTEQQKAQLHNSDYVRSVCFSSDGNTLAFGCDDKAICLWNVKKRQQKAYLDGHDDCINSVCFSPDGNTLASCSSDNSIFMWDVQTGQQKALLDGHTNDVMSICFSPDGNTLAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDGHNNDVMSIRFSPDGNTLASGSSDNSICIWDVKTGQQQAKLDGYTNDVISICLSPDGNTLATNNNNSSIRLWDLKSGQEIKFSDTNYQDILLQFKLSLFKNLSQYLQASNNITILQISQTPLFQAKGAIILKGDFVNYQGQNLRSLFKSKGCCFLEDLKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QWX7</accession>
    <name>A0A8S1QWX7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T2730001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000282">
        <property type="protein sequence ID" value="CAD8118930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="31"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="27"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="260"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="302"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
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            <begin position="344"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="386"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="428"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="471"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="513"/>
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    </feature>
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        <location>
            <begin position="555"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="597"/>
            <end position="638"/>
        </location>
    </feature>
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        <location>
            <begin position="646"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="681"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="723"/>
            <end position="764"/>
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    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="846"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="888"/>
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        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
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            <begin position="902"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="930"/>
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    </feature>
    <feature type="repeat" description="WD" evidence="3">
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            <begin position="972"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1014"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1056"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1098"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1140"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1182"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1224"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1266"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1308"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1350"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="1358"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1392"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1434"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1476"/>
            <end position="1517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8118930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1595" mass="175662" checksum="BC866008B8B3806F" modified="2022-10-12" version="1">MQGPFEDKEALADIFTQVKDVDEQLFGVILEILRKEKVKDCIGFLSDNGNQKQLESQMLKQGNFTQADTEQKLSVVRKDMIQITDVLKKLKDHDFNNKDFSTEENYESTLDLIKIIKDERQAIKFLIFLVHLTAIDERFIRCGSNSLYLLVEMKADLTKKNFENIKISNTQLIGANFVRCNLNGSHFENVDISGMNLSGAQLFYCKWKNIKINELNIFDCQEGSVKSICFSPDCSTIALCCKDKSILLQDIKTGKEKFKFDNHSDWVFSACFSPDGTTLASCSKDKSILLWDLKAGQQILKFDGHNDWVYSVCFSPDGTILASCSKDKSIILWDVKTRQEKFHLNGHRNDVNSVCFSPDGTTIASSSRDKSILLWDVQTGQQKAKLDCHSDYVRSVCFSPDSTTLASCSKDKYIRVWDVKTRQEKSQLFWHRKNISSVCFSSDAILLSYSCKDESIHMWDVKAGQQQSKQFEGHNNDIYCVCFSPDGRILASSGRDRLIWLWDVITGKLKMKLEGHKDCIRTVSFSPNGDEIASGSNDNLIILWDSKTGQLIRKLEGHNGIVLSICFSPSGSILASGSRDNSIYLWNTQTGQQQSQLYFNEGYVQSLCFSPDGTTLSSGSSDSFLCVWTVKTGKEIVKKDCQIGIIFSVQFSPDGTTLGSGSEDNSVRLWNSKTGEQKLKLFGHSSYVRTVSFSPDGTTLASGSDDYSIRIWNIKTGQEKSKLDGHHDCVLSVCFSQDGKKLASSGIDVSIFIWDVQEKFTLDSLQDYMSLSCISPDGNTLASGSYDKSIRLLDVTTGQQKAKLDGHTSAIMSLGFSPDGNTLASGSYDKSIRLWDVTIGQQKAKLDGHAGPVYLVCFSTDEITLATCSQDNSLRLWDIKMGYQKAILNGHTQPVYSISFSSDGTKLVGCSSDKSIRLWDVKTGQQKAKLDGHDEAVISVCFSPDGNTLASGSYDNSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHTSTVYSVCFSPDGNTLASGSSDQSIRLWDVKTGGEEIQSSDNRYKEILENCKISLLQNNSFIFTSNITILRISQTPIFQTQRAFVLQGEFINQQGFNLRPLLQQRGSCILESCMQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN49</accession>
    <name>A0A8S1JN49_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050527</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050528</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JN49"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="111" mass="13323" checksum="1E78ED0C2311CD08" modified="2022-10-12" version="1">MVNFNDLKHKKKAREGQEIQVQNIQLEGIINLLQNLEKDLDYIKGLEAKEMIDSEDKYKRIISYDLDEIKETCQPIKINEKHQKKKIKKQKAPKKFFEKIVKINRIKRENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQU4</accession>
    <name>A0A8S1JQU4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQU4"/>
    <dbReference type="OMA" id="ESYHIAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="614" mass="73652" checksum="02C4E2D0D43035F7" modified="2022-10-12" version="1">MSNENYLKESTKLIRKGCPRKNLQLLEKVKYNNLIVNKSQEMEILRRQVYSLNQILESYLNRNVSFYKKTALIVISKAKLFFQQWYELIRSQFKYKKPNIKMIQFYLYELLKYTQNMSIKFHREESYHIAYYYCNASKQVLEQLNGFKFKIWQHSTRDVWTDLIVGNMLLQNQNYELASIEFEDSLVRIQKNISSIISYKIEKSLPKQEVKKYAIIQMIMLFHIFFLLCISYLYSDNLSKYQELLKLLYYLNKHNQFNGEQSKLLKDLIKQHNLKLQIYLQEQGEIQKIMSLMYDVPETKAEEKPNYIDEHFYKKYQIKELNDQAFFRTKYQFTYTNKRCSIILKGKDKRLNSPTMQQTQPISQYYDTTIHPNLKQSQNLSLSPKSNFKQQSFQKKNIKKESFSYIKSDRESMKYLDTQILGSLRKESFYSYNELSKDMETNISINSDEQKQLKTERSILRSKSSREHKQEINQQTKMAIFNLIEAKSLYYMEVQKANSKLKNIKSLKDQQQKKMISDKLISDMKYIQKQPSMLMSTDQLEEKDSKQIYNDVFNDLKKLADNYVSKLSMYEKSQTRLRIYTQQQQGMNQQSNGNTAISKCKELSKSIQQQKEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JS16</accession>
    <name>A0A8S1JS16_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VASt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0090402</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JS16"/>
    <dbReference type="OMA" id="FFIADNA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF4">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="4">
        <location>
            <begin position="145"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8045404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="420" mass="49024" checksum="76CA55D527388C61" modified="2022-10-12" version="1">MSAKELLIQLFALPKGEIIFQDYSCALKSLISKYGRIFIAENHICFYANLAGSKINLVFKLNDIQRIESKNKNDIEITLNNNKVYCFTSFQNKDHVFNFMNSLLQGQPLSTFSDSQVNTESSNNDQSIIDNTDVEIQFLKSESSTDQEICKFTFPFSLDKFFDFFIADNASLYSVYDHRQNEKDTDMNITKWTVNEDIQDMYQREMKHVMKLTGVPFKDKTRMHKLFTYKRDQQKLIYTCTTHTLDVPYGNCFQAEEKWEVTQPEDNKCLLRVFVSVVFTKSTIMKNTIINRTMAGYKEDYEKWINNVKIRLEAQAKQSKSSVSYSTHEYEESMKLDNENIFSKILKNSNSDLSNQKEKSGLPSIITQKEACFVLLITIMIIIMLIQIGMMSNQSTRLEQLEQIILQNQQQRFQLLNDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTF8</accession>
    <name>A0A8S1JTF8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTF8"/>
    <dbReference type="OMA" id="ILCDDEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="30"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="24360" checksum="7F2AC7C4BAB317CF" modified="2022-10-12" version="1">MILCDDEQQKQKYQRNNNSQDQLKQITEIVIKKQQEFLGLEKQLQKYESEVRQNIRDQYLMQQQCDDLKSRIDELESSRNEFLNNTKNIIIKFKRENEELYQKCKSLTAQLQKIDNQSISKYSTIDNTIMTNLTNIVDQKKPTKKTNTTPQKLKTDFDVSEKRLSQPIFKPNYFQKKQSIQISIQNKKKSFQQLENYLNVTTSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JUK6</accession>
    <name>A0A8S1JUK6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0060168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JUK6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="108" mass="12904" checksum="0A938589C6CA4495" modified="2022-10-12" version="1">MDQKALSYKQPSSLSQNLQTAANSSQNRSPQLFIQIMTRIIDQKYIPKEIRDQLQQKINFYSQTYDLNEDIKYVQGQLQQLSYIQNQYKNYESSPIFQLIWFQIQSQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0W6</accession>
    <name>A0A8S1K0W6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0130117</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0W6"/>
    <dbReference type="OMA" id="FQNQYMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000003">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25427">
        <property type="entry name" value="zf-CCCH_UNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="78"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="78"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8048875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="223" mass="26114" checksum="97F57AB296802DA6" modified="2022-10-12" version="1">MQINQVQFPNQKYKTQLCRHFITNGNCALGARCQFAHGRQELRANANGFQSNSDFMMPQHQQGTLPVKIQPINPMITNYKTQLCKHFNPQTGICKNGVTCTFAHGEQELNQINPYFQNQYMQQVVQQQMKQQNQTQIQAELTQQIVIMILINMEHIFPDQQDIINLLRQGQEKAQSGDNQGASEIIKLIIHDEQRTKEEKQYQQIYNNAQRYYDSKLKEYFNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2L2</accession>
    <name>A0A8S1K2L2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0130232</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2L2"/>
    <dbReference type="OMA" id="YYQAWLD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="10">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="296"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8049107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="384" mass="44926" checksum="63E3463D43DB39CD" modified="2022-10-12" version="1">MSKHLQKFSSFQNFQKEQQSSNRLLYVYRNSVYDLTEFADRHPGGRAALNIYKGRDLENIFFNPSIHKHSTNALQVLEQYKSGIIEIKQQSSIFNSKSTNFTISQISKKPQISNDTKTQPIKKTPIKSQSVKPKLSQNEDRLNQSMNNLNQSMTSSVKSHFLHIPSQMEIMQLQKQKQKQLIQTWKQTEIDELKRQQAEVLRQQQEKNQPNDDRKVQSEIISTQKKPNNSYYQAWLDKKKNQSNETSTNNILPSYLTQNNRADDQNSIKNDTQNYQQILSQSVIVQKQQKKVNPYYEEWKRKQAQKEHQNNQEQLQQSIILEENESQLSGQPSSVNNSFQRSFSLISQKTIVQENPQLYVPVFSPKFISPLKAKFYHPISKLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3W5</accession>
    <name>A0A8S1K3W5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Elongator complex protein 3</fullName>
            <ecNumber evidence="15">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T0140044</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Catalytic tRNA acetyltransferase subunit of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ELP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="15"/>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3W5"/>
    <dbReference type="OMA" id="KICINAK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000003">
        <property type="entry name" value="Elongator complex protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="17">
        <location>
            <begin position="85"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="16">
        <location>
            <begin position="399"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR005669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8049547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="551" mass="63195" checksum="6D4E649D385BA8E8" modified="2022-10-12" version="1">MEQKEKKGKKQQDSTVDKLKCISEIVAELIKQYDSNQKINLSQIKAEFSKKNKISGAPKIVEILSAIPDDYKDKLIPILKAKPVRTASGIAVVAVMAKPHRCPHIAVTGNICIYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYNPYIQARDRIAQLKQLGHDCDKVEYIIMGGTFMSLDQEYRDYFIRNLHDALSGHRSQTVKESVQFSEESRSKCIGITIETRPDFCQKSHLSDMLLYGCTRIEVGVQSIYEDIARDTNRGHTVESVKKCFAMSKDSGYKVVTHMMPDLPNMGVERDLESFKEFFENPDFRPDGLKIYPTLVIRGTGLYELWKNGQYRNYPPDFLVDVVAKILALVPPWTRVYRIQRDIPMPLVTSGVDNGNLRELSLQRMKDLNVACRDVRTREVGIKEVHHKIKPEQVELIRRDYTANDGWETFLSYEDPSQDILIGLLRLRRLDKHTFRKELIQEPTSMVRELHVYGSVVPIHSRDPSKFQHQGYGTLLMEEAEKIAREEHGSTKMAVIAGIGTRHYYRKLGYHTEGVYMVKKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K467</accession>
    <name>A0A8S1K467_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K467"/>
    <dbReference type="OMA" id="RPNKTEP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="396" mass="46229" checksum="3324EF006C1D9BDC" modified="2022-10-12" version="1">MYDHPQNGLMEAHSFNLDCLSQDIQQIKQTLQGWTSIFSQLMEITIETHKQNQCNASLLQQLLATQHRRHKYSQPKDQIKLSPIRPNKTEPDEIIKPDNFSKIFNNNKKISIMQLQGTPFDSAFESINKELEEPYREDQYLSVPKSMQMSVDLNKQKKNQHLELQQPKSAKNTYENKQFAFNTKVMRSQDIKANKENHQHHSSMNKPDSKRTLDQPQLSTVVEENQSPCMSPYENNFRYSIAQSHRQYDQCSTAKTSQNSQRPLNYFQQQQLQSENNRSSSKSCYGNQQTQVLSNNTNIINQQQQTQQQSTSQNPFLNSLNNGSTKNIKQVQTSTSNIPFKQQFEIINQISNQRAKSYSEQNCKKQLSKRCLDIDLFDKQYETSQELIWKAQKTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4E0</accession>
    <name>A0A8S1K4E0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4E0"/>
    <dbReference type="OMA" id="DNKMQIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="232" mass="27781" checksum="FCA2DF7519634C02" modified="2022-10-12" version="1">MNSQAQFRQDNKMQIHFSRPSSRNNSFLPEIVQTPSYNQYQKQQSNSIFNSSKGMDQLNLFKISQTESNIDQITSRTEIQRKELIREIFRQDKKHIALKPLIKQQEYTHRNRLRRDDQIEDQQIQLKSILKRKSSNSREHVDIFDFFEQDNQDLKQQQQKPIFNRLKHNKQVSLQPIEKDDYSPPLYNPGKKIVSFNKQIQIKVIDPNEEKPKSNQKTFRRMYTVADLLDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K658</accession>
    <name>A0A8S1K658_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0120096</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K658"/>
    <dbReference type="OMA" id="MWRVEIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="7"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="199"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8047786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="290" mass="32986" checksum="BF9FA6FFBA3B025E" modified="2022-10-12" version="1">MWRVEINNFLYSFLAGGASTGLIQLFQTPVDLMNLIIYSQNQNQPIPFVQRLFCYLQLLQEDGIITYLCKNGFQKIMRSALLSGVHFSVKDTLKSLFLSDHEVTKNTRKLFYGTLINGGISGIIGLLTVYFLDFQMIMQMNNAGPQSNRNTTRSNQIKQVFIGLGLSLPGVFVYRAFYFGCYDAGKQILWGKAVKQRKASFLFKFLLAYISTFIASSFSYPFDTIRRRIMMQQNTSGSKKIGIFGVIKQIIQKEGLSGFFKGNSITIGRYVNQSLALVLYDCFQKIILRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9V4</accession>
    <name>A0A8S1K9V4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0180096</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9V4"/>
    <dbReference type="OMA" id="NKGRTCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="105"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="143"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="105"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="143"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8051437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="234" mass="27501" checksum="A58DD93A2676F894" modified="2022-10-12" version="1">MHVTNKGRTCKQRSSVHTKDQKITSERRSLNVDMNNYTPKQPKWAEKTIKNMNRCSFTDLNEQDKRGSMSTVATLTTMSTDFYVQDDDPIEEQTSSSDESQSYVKFKTEMCKNWSMLGRCNYGNKCQFAHGQNEMINRQCNQKYKSKLCRSFHQDYVCFYGARCQFIHESRSVDQIRKDCKSQTSFYQPTSNQLRLKSFQLITSDWKQEIPLQECFQLWKTKLLLQINISSSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAN7</accession>
    <name>A0A8S1KAN7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0190068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAN7"/>
    <dbReference type="OMA" id="CFGQIEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="207"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="411" mass="48875" checksum="2EBC056DD6D91336" modified="2022-10-12" version="1">MDQIKNVDSYLDNLLGNFSATRCIKYAQQLVSTKSQKIIEELDSRQLFINQNQQHLDITFRSQTAQISIPTRKIDCFGQIEVKTFTKEEIDSLRKERGQRAQLKIRQIQEVQEKRKQDFLESWKKTQQIQHQKPLFQILQEQENAQIKLQQEIIQQKLEQIKQERKPLNKIDFEEHRKKFCDLIIQPRKKSIEQQFNNQTKSISPEKSTYYLKIEQEQTKLKLLEQERKNHLMELQNKKKNFSEETKKRFKPKLSLDKQNELQEIQSKLHHKSDFQKYQVLANKVRQKKISYQIQDTDEDAQSLINFIKNKDQNTSYDLASQDTQLPKFKHKSFVIETNTQQQNTNYWQKVALNNNLSIKDKNQLIIEEAKKLEEMSKKQEQLARVTGNDSPQADNLLISSIKAKLTVLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KG56</accession>
    <name>A0A8S1KG56_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0200035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KG56"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035922678" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8052855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="24370" checksum="7C8DD8D7DDB79FF7" modified="2022-10-12" version="1">MRIIILITLIIIVLSSENTGLRIDLNVEIPLLENQYVIFNATQQQEGASSQILPTYKLLANLKNNSLGIYSQMFDFLQYTNQSQINCYQQNVVVLTDVFSCTQSCNSTPDTSNLGIRNISYVLTDEKDEQLQYLVIDNSQLPCVNGTFFKLIVPWITVQALFKFQDSSYLIGTIIIVLLVTIITIQIMSIQSIILQIKEIQEKNKKIVRKKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KGX0</accession>
    <name>A0A8S1KGX0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KGX0"/>
    <dbReference type="OMA" id="IRNQMCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="316" mass="37584" checksum="5EA7B0085F9C52F0" modified="2022-10-12" version="1">MYIIIAIGIILLLCFIIQLTKLNPGKKFFLIDIFLVVLVGMIIYDESIYKFNDFAEQLQIAFQKQYPIEYKLDELIQKNNLQNHSYHLYYGMHSEGISTFFKFMINKQLKEKKQAIYIDIQSEVQTQEQALQLFKAPNLNELILFTKLKPTLIAIDNLDLSLTKKYCYICQILSNLHQSNQTTIIASAKNLKKLNLMLDDYNINVMEIKQYQHGKQNNFRYDWYEQSLQSNKRYEDFVIQKQREFISELNENELQLMKIIRNQMCKIKCENQEIRLDDVTYQFGDLKNLIDKNILIATFGQLKFYNSFIFDSLQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJT3</accession>
    <name>A0A8S1KJT3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJT3"/>
    <dbReference type="OMA" id="SIYCQRY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="181" mass="20897" checksum="9D06BE110FEF15A1" modified="2022-10-12" version="1">MYGNKNKNAALISIYCQRYKMRLNSTPHVITNQLSINHLSGQRVQTEQNQSRRIKTIGSQRSKLYYKSESREPIKSSNKSKQSSSDSRKQSNYDSHVNTSTSDNEDKVLMISKIVFSVKSPLQSLRFQSFILDKKAQNIEINRKKTLRNSLLSNSFKNQNFQMTGNECGWSRKSSESLIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLR4</accession>
    <name>A0A8S1KLR4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0220211</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLR4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="212" mass="25520" checksum="AC319CA55EC4E0A8" modified="2022-10-12" version="1">MKRQILNEQLKMKKNKSCQISRSFQNEFSLERKYSISQKKGNNHTSGSMQIFEMWSKKMKKQCDNYLQSLKHKQEQEINKSNHVPPIMLKNIDMMKRSDKTTLPRSRITSFNQQDLNQRSIKQIIQQDQEYIGTSIYSISGRSNVQPFFYQRHKLSPIRDIKNCIDQCDTLLQDVQSISKEFYQPKPDRFKLYVQKRQKEKLKEMFLLRRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQ91</accession>
    <name>A0A8S1KQ91_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQ91"/>
    <dbReference type="OMA" id="HYNGARY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="39754" checksum="A12F69951D742FFE" modified="2022-10-12" version="1">MGQCKCATQCCQKDELTSQKISTRSIQVSIKHHKHQKDQTSDKIDALIQDDLDSGIMKNSIHSKVSIQDISKNLISTQQPIPQQVIETIQNKERKLLPIMLMPNGTKYEGQWLNGMRDGYGKQIWPDGSIYEGQWKEDKSNGQGKLIHADGDIYDGEWVDDAACGKGTYVHFNGARYEGEWLNDNQHGYGIEVWPDGAKYEGQYKYGKKNGKGQLTFVDTANYEGNFIDNEISGFGVYKWPDGREYIGYWLDNKMHGEGTLKWPDGKQYKGNYQQDKKQGRGVFYFGDGRKYAGTWINGKQCGIGIYYQSENQYRIGMWQNGQRTKWLKDEEMESNKEQINQLLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSU5</accession>
    <name>A0A8S1KSU5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210264</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSU5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="156" mass="18128" checksum="318D97CCCB33E9F1" modified="2022-10-12" version="1">MWAYENQRITDKGIDRLFNGISLLNNLESFELNMKGWGDGNYEITDNTIIGLSKCLKKLKNLSEIKLILWKNIGLQATKQLNKTLSNLKNLTKIEIKFESCLQQQIIQNDNIDQKLIQIKLNAIQKRKLLFQVEGILVSLEQLIPKFTLWDIILKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KU48</accession>
    <name>A0A8S1KU48_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0250087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KU48"/>
    <dbReference type="OMA" id="CAIQIAV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8056852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="231" mass="26879" checksum="A663BCF05348913C" modified="2022-10-12" version="1">MKEDKGAQYVYYNLVTSSHKYRKALGDNIEIAQLKYIDKQINKNEITVVMRFLPVNFNNEKLEALQNIRSEELFTYQGLKYGIFKTSDYEIAENIKKKIISYFQNKKYNDIRITIKAKKKSTKEKIKAFLDEESYNPRSQLEQKIGMPLSPLKVPPLVVKEIEQPKIIPTVIQPTICAIQIAVPQIQFKQVKPAEIKPTIIEQSDQKLIARAVQLKPVTLTVHRERSSRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWK6</accession>
    <name>A0A8S1KWK6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0290203</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWK6"/>
    <dbReference type="OMA" id="IRKSYIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115:SF1008">
        <property type="entry name" value="KINESIN-LIKE PROTEIN SUBITO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115">
        <property type="entry name" value="KINESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4 5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="7">
        <location>
            <begin position="28"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="714"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="729"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="752"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="111"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8059860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="773" mass="90324" checksum="E276CAD893D14781" modified="2022-10-12" version="1">MEIKKKPAIDLPTSETLSVLDQNEPQNGIEVICRIRPKFVGENYVNYMITDKRLDIYETNNDNEISNQKTFTFSQIYDQNSTQRNLHQEYVIPMIKDIFEKQKGVLVFTYGVTNSGKTYTIIGTQEAPGILPLTLQTLDKIKTAFLLNKTTIEIDGQVIKIGESPYDDYVLKEVNYTFQSLEIYNEEIYDLLANNKTKLQLKEIYDKKCYVKDAVEKEITNNQQFKELLNKALTNRQMGETTLNNQSSRSHTVFKIQISFLYQQEQFTIQRKQTICIVDLAGSERAKKAETQGSQLTEACNINKSLLVLGKCLKQMRCQNESDQNSRIPFRECKLTRLLCEYFTEENKILMIVNVRLTGDDIEETLKVLHYGALSVNVNLLKSKIYDSTISYNQRLQINQQQQVTNDSKQKKIPKRNAKEIIRRAKMLVQNTTQHMLFQQTIQDSFVKDLISTVKQQQARLKQFSGLDDELFYPPILESDQRLQKSLDKIQSDKSRQASLDILENKGLIVPPAVSNFFEEIPIRKSYIFEEPFVQNEDAIIDERPKIDQQRQSGILEEKQNIQSIIEQHFNCYDEQDDLMAGFRQLDLEQIDEQTDDSKQQSKCHMIKQNNNQENDINNSNQCFNQIKQEDLLKYSNQKLDKNHFDQATNTTPYDELKKKLFGQLFEQELQKHQEEKDKFKQEVMKKLFNDDDLQSLIEKENQEELINLEDSAKKQQQRNLEKLISSNHKSDLKVINESDQEHDSSGDKKQHQTKNHPKKKKQTKKKGKSKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXJ4</accession>
    <name>A0A8S1KXJ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0290265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXJ4"/>
    <dbReference type="OMA" id="HAKTFIP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="161" mass="18216" checksum="242E86D30299FFB4" modified="2022-10-12" version="1">MTACNFAWSCMATRPESQYNYIFLIEIGFKILLQITEIIVIIVSFALIDGKRKIIKQVNDDGCVSDPVSDYFFTNLESDLTKFAWSYNLANLVIFAVTLILDLIIIKHSINQGHHHGAKKHHEKLEHQELDVGSNGESGNQQEVKSDLKLQVKTEALQTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1V6</accession>
    <name>A0A8S1L1V6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8060971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1V6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="109" mass="13269" checksum="E7AAEE7C0FDE0086" modified="2022-10-12" version="1">MHPNYRGYHEYILSLFSNQNLNQGNIILLSTLQQILHLIYQGLMILRLQSIQTWKRYIIMLQFQNSLNMIKVPNWMFDIIGLFFDYCNFHIFPKKKRNRNRNNLYNLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L503</accession>
    <name>A0A8S1L503_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0330196</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L503"/>
    <dbReference type="OMA" id="WIHAICF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140575">
        <property type="term" value="F:transmembrane monodehydroascorbate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08760">
        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09631">
        <property type="entry name" value="DOMON_DOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045150">
        <property type="entry name" value="CYB561D1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045266">
        <property type="entry name" value="DOH_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422:SF24">
        <property type="entry name" value="DOMON RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422">
        <property type="entry name" value="OS05G0565100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035712435" evidence="12">
        <location>
            <begin position="19"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="14">
        <location>
            <begin position="161"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="15">
        <location>
            <begin position="276"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="13">
        <location>
            <begin position="521"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8062557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="884" mass="101921" checksum="DBEFD3FD77A0344C" modified="2022-10-12" version="1">MNFTIIPCLLWIIINCQAQQISQTVEFYEYEKLLNIQIILSSQFDFQLTFYNNKTHSFEILNCSNLDRCECESPKFSNYYCFENNSEFHFHFSKLPYVYGDTILKRENTTQIQNNHQIESYSSNSHFRILDLEEYLNKSLAAQSVTYTDSDLTSSLQLGTYELFMKWEIYSNGSFDMAIDYNQLNWIGFGFCPTMSNCDMIVLYVSQQTVIVMDTFSYGQSTPRTDKTNDIDIISYAVNSTGYKVRFNRKLDTGDTADQVLVKGDTYTFCVAWSGADQMEMHSAWYNFDIIFDNGIIGQATLKSGESILYYVHAIVLFIGWGIVADLGIIIGRFFKSINSYLWIHAICFIFVDLSTLVLVALMLFVGGEEGNVIEDGTLEAHKIISIVLGITVIIQHILGVVVKHFLESTGKENRQALFTIRLIHVILGTIMYLAAKVDIILGFVKNEEKLLLALGIIWTVLLILVRIGLEIYKSFNSPIRTKIAPQQQQPLNENQTKLLKLLQGNHPTANIIKELPQIKWVLLNQDVYDVTDYQHPGGNFIISNVNGQEISRYFYGAFQLESTTMEPYTHSQFAYQTLQKRYIGTIQSVNYNNSNPASFWDLIEKVDLSKNISLFNFTNPNFQINLRQNIYDLGQHFSVTLQEVGGKIRMYTKVLCMSNPYQNFRDQIIKYHEGNSTQLPVLQFDKINTIPLIIKKYNFPKALSAQIFEHQGNFWVQGPFGRGLELEPKCKCIAFVGGTGLLPFLDLLDHLLIKSIYLTNKQKQQQIQQFFPQYDQLDDSFEFTLLASFQNEEEFIGKEWIRKLYQINKTNNLKLFDMQIRYSDSTLDTVIPAFRGRFTEDLVKQHLGDYNKKVYICGPPNMIYSLTQIISKTEQDQTKVMIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9V6</accession>
    <name>A0A8S1L9V6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diacylglycerol O-acyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0330208</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.20" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9V6"/>
    <dbReference type="OMA" id="FEMVQIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF7">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="212"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="340"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="464"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8062582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="550" mass="65089" checksum="2DD8F86E6DD8284D" modified="2022-10-12" version="1">MIKVYNPELLISDAQDKLTQLVRDDFKPFILRCEQHKLCVLENENLILFSVLFDKCSPIVDEAQLTITLVSQKDESKIIAIKFADKKGMSYLQHYIHSRKTYSKYMGPLAYQPVRKERTSLLSTESPEQNFRGILNLAILILIANHIRLIFENFQKYGLLITNVFWKFEMVQINGNPIMQALLVLIINLLSGFGIQYLQFKRDLNPSIIRTLNVFNIVLTIIIPCYVIQGSHPGLNLIFLGVQFIVFMKLISYAHFLRNTYFYIQRIKQVKNKKVTLDHFFAEYEVNNENLKILEKYSQNQSRILDFKHFLYFLAAPTLCFQLSYLRTNSIRKVWLIKRIIEYIFVILFLVIIWFQYTEPLVENTYKMLSKAPTLMVLVDRLLKLAIPNTYMWIALFYGQFQCFLNITAELLRFADREFYKDWWNCKNLEEYWRLWNLPVHHWLVRHIYFPCLKSGMSRTTSNMIVFLVSALGHEYIVSASIGVVEAWAFVGMFAQAPFMLIQKKLEKLLKLQDSQLGNLMFWMTFCFIGQPIMIFVYYFRYLEKIGHPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBV8</accession>
    <name>A0A8S1LBV8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0350124</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ICKNECH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001470">
        <property type="entry name" value="Interferon-induced guanylate-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003191">
        <property type="entry name" value="Guanylate-bd/ATL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02841">
        <property type="entry name" value="GBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GB1/RHD3-type G" evidence="5">
        <location>
            <begin position="36"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1091"/>
            <end position="1115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8063755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1636" mass="194143" checksum="945CF9D13E9C5147" modified="2022-10-12" version="1">MKKSFREEAMPLIFYQQETQTYQVNPEAIDIIKNIPAPIGIVGVAGMYRTGKSYLLNRMLLNRSDGFGVGPTVLPCTKGLWMWGKPLLGQTSDGESCSILVVDSEGLGAPDEDSTHDIRIFSLTILLTSCFIYNSVGSIDENALQNLSLVVNLTKNIQLKSGQSTTDLEDLSQYFPQFYWVVRDFTLQLVDRNNEQITSKDYLENALTLQKGTSDGIDQKNKIRKLLSTFFKDRDCITLVRPLTKESSLQNLENLDFDKLRPEFFEQVINLRKKILNRIRPKTLNGKTLSGQMYCDLVKSYVTAINNGAVPAIESAWTYICKNECQKAVAEAFETYEQILKENLHNRFPISNEDLKAFNRSLKEQAFALFKKKCVGEYDEFKNELSKRIKNRFAAVKQENDREGSRMCSQFIQQEFQPIDRKLKLGEYRTFGEYEKDIKMFYNFFIENGPRVGTRNQIILEFLQRALIEGSNLFIRQYSQETEMMKNVSQETQKKLEQELKEARQDGLKDKNNLLMKLAQIESEKTEIELREQVARDNLEELKIQKDQVERDLKLESENEKNELMRQIQELKGQVLKAEEFNKDLERTTLFGNSEFEKERALLEQKITFFEKLVNEMNAKEIDYQNEIKNLRKEYSLQSKDQQSKADQSVRQLQQKLNDLQEKLNEMENELIEKETNFENDFKKFEHKERSLTKQNLELNEQIQVLTRELKEYRRNEEQVQLMLKNDANNQVNEMIEKVQNLEDLIKSKDDQLKQTRSQSEKDKALMQQKMEFMEVQLDEYKKQIEENKKSHEAIMKAFENSSNESTYKIDAAKMNELREQHKRELKNIENEYENTKKRLQQQIDQLNEKNNELELKVKFETGDLIKELDNLKEQLQVTEEQRNKLLDQNKMLDGQKLQLLKEQEIRYQKKIKQLEQAIDESDAKIAKEVNLAQAKAEESLTQLKNFYEIERERLERRICEEKEKSEKKFQAAQEEFYHKLRETEQNYEEEIENLKDDLRDQVQQYTNTIQQYDHEIALKQQTIEIFEKHIKETKEQLISLQNNNNATLEQQMNSFTNERKSLIQKIDVLSSQLNNLQKEHMALQQKKDLLENEKSRKEQQFEQSRKEWHEEKKEQLERLEETKLRLQKMNDEFLEKKIEYGRETALIQQQNEFLQKKIEDLQRQIDTQQSRFDEKLKQQKNEYLVELEQKLERAQEEKTTIETKYEKIKKQLKEAEYQYNKQASTLEREKAILTEKLGHLEIRKNELESKLKDEAATIAQQQASLREQIAQEKKQLQQELEKYKQLNLQLEQDKSEIHTSYERDKALWEGKFQFLEQQKEQAKQDLVDALKKFEMTLMHLQRARSNEKDEQENNLNELLLSVERKYQAQIEEANQTHQRIVQDYEDKIRRLQKEIKTHKDKILIDQHGKIGNQLLSEKKFAEMLDNEKRLQQEIENIKQDRDQKILEYQRMLEQERESLKVKITELETKYKEVESKRSTLIFEFEKERAKWNLDRDHLNNLKNELTDQLDRLRKKEELLLRDNEKLKNEQRATRRSVAAHNMTSNNIMTGNKYRNPINNVSTIGLQKLSPTHSNNTSTSSANISVLKKTNLADITNFEKVVPPTQQSFQNNKYYFYGNQQGQNDDSMIGQSDFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCP1</accession>
    <name>A0A8S1LCP1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0340247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23022">
        <property type="entry name" value="zf-HIT_DDX59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000002">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF375">
        <property type="entry name" value="LD29171P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="1">
        <location>
            <begin position="568"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1613" mass="189847" checksum="59AB186E627A26BE" modified="2022-10-12" version="1">MSLSLSDLINQSLDKIKKAITKKQFELKQTIDEVQQLKDYYDANHLIKVYQQCIESKQVKLMELALFDIKNLVEQGFFAGEQSIGDKRVIDVILDIVISCQQEKEETVQIHMIKAIQTIMTNRKHHIYGELTTKVFQLLINIHSVSKIVIIVNTSKEACQKIVSTYFQRLEDYGILTEDEFQQAIQKQENQGQLILGKCRAMVNAEQYMRSLMTSLIDEVQMYSERKQIYDRQIEDLNQMKVIDLNLQEPNLRNVTVEKNQIQVQVINEQNIKNGKFGWCVVCRKQASQYCKETKVPICSKECKFIHLNQMNNFSQCYQHSNNSELYCKDAQEILEMLCQLTQRDNSNPQLNQIAIKCKILSLELIYEALAQSNTILQNKPKLIQVIKEQLLESLLKNSLSTEKQLLILTLNIFIQLIWKVRSHLKKELEALIENVYFKFLDSSNSSFDHKQYTLKVFNKIMTKPRVVIEIFVNYDCSLGQNNLLKKILDMQCRIIQGRFSKQEFQASITQNQETYLKSLCLDNYYGYIKCIKEFCEQNEDTYNIVQVQQLDDQEDTTIQSQQLSQDPIEKQKQMKLEMNKAVQKFNFKPEHCIKHLIACQFMETRDPKLFAQFLWENRDLNKDKLGELFGCSSEFNQQVFQQYIDFMNFKDLQVDEGLRYMLEFFTLPGESQQIDRIMEKFASKYCIDNPGIYQSAQAAYTLSYLLMMLQTDLHNEKNLDKMTIPQFFNLAKGINDGENLPQDLLLGFYQRIQKTPLALHAKEQAKRALEQANQVDQRKRHAMLAKEAEESLKKWFKEHPNQDAYFYANSIDHVKSLLQQTWSPIFASISVFLEQTEDQQQILLCFETIQSFIYLMGRFDLDEEKDTFISFLQRYCTGIPNTYRQILGVQALIKAAIQSGQYLRKSWRVALQLVSKLEILHQVVKKIKVDSPQKESYNQEDIQNIERLFQSISYDQIDKIFNMSINLDSNSILEFIRALCELSKEEIKQNRTFLLSRMIEVADFNMNRIKIIWSRMWEIMREHFLEVGCHKNVDLAIYAIDQLKQLSCKFLQQPELANYHFQKEFLMPFEQIFSHSQAQQQYKIQLREYLLSCMCMITNVCFNSLKSGWKIIMSIVNQALQEDQQQLIRLCVQITDKIMEDVNNQQVNQEIYMELIQALIKLTKNKDIIIVENAIKQLKTLVDHIALIKKNDNKFLDSLWIPVLSSLSILYSDERVAVQQQSVQTLFELLQKHGVYQSNEFWKMILRGVIRPLFDEIQFSKLQYTKQQQSKKSIASTCKMTFQLFTDLVISQIQQMQPCINDLIDIYIQLVLQTQDHISILCLQSLKKIIQNVGEYLNEDNWNILIEQIQHLLQQCSPTELLEAFNLDEDFQKPLDELLKEEIRPKKFSFKINAQECSSKQSIQERCLEILEVQVTQFQNQITEQNKQLILQLFQEQYQKCRKFNTNLYMRYFLEQWAVQWNFVKRSSQEFDDLDNTTQSKQLSFINIELLAAKVIVFLSNDPFSFIEQLIQRFLDAYIGFSQPQHRQDTLNGNLEQLRHLESQIVISRLQLLFMETVFPFLKQNLKSKVVSKWLIQLLKAGLNVSGVENKEYNKVLVNLLEEIINCENNVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCW4</accession>
    <name>A0A8S1LCW4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0360097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCW4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8064381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="74" mass="9165" checksum="F84A2B9C2B9B78C3" modified="2022-10-12" version="1">MIKQVYFFQLKKNFKILYRCENKKETLKSLYLFELEYFNRNCIGCKKRRTNILYFIYVIIIKEDQLILVESIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJG0</accession>
    <name>A0A8S1LJG0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0410217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJG0"/>
    <dbReference type="OMA" id="LFYFKLD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="249" mass="29454" checksum="5C8ACBC6311A8288" modified="2022-10-12" version="1">MGCHQAKIKEEKQITESQIKIKIERLKQFAILKKDQDISKILEQEQQLNLLLKSNQRNKDQEYILFSKLSQQIKYLETINFIIVYCQLLSDQVLKILKCQGNTNSLQELMIYIESVIYASEKLNLKQAEEFNLYFLFYFKLDVNNLRNVNPKIIQMFQSLLPNSYEINDYAKSFANKYGISEIELNQVGHQFILNNLISQQINNSNNFDFQQKNPFENLKINDENKNNNFNTCSIPVKHNKKRILDLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LNY2</accession>
    <name>A0A8S1LNY2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HPt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LNY2"/>
    <dbReference type="OMA" id="YLPDETM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="22763" checksum="BF322D60AE0F2D22" modified="2022-10-12" version="1">MSNHFPQESYDIFDVQTALEYLPDETMVKGAITLFLTYFQDGEGFDELINLYEKGGWQLVGTPAYLKFEQLAHSIKGSCRYLALHRLEKKLELLQFFIRDGKEIQVASILNEVLEQCPYAQRAVLKYMEMPDEKVRHYKFVSPFYDVYGNVHICHKLNYTFQKQKNKMSAIHHKQPYQQQIINCTDQDQCNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQ82</accession>
    <name>A0A8S1LQ82_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0420021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQ82"/>
    <dbReference type="OMA" id="RCIQQHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1720.10:FF:000036">
        <property type="entry name" value="Palmitoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000737">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024453">
        <property type="entry name" value="Peptidase_C92"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47112">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47112:SF1">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05708">
        <property type="entry name" value="Peptidase_C92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="53"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8068171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="363" mass="42837" checksum="C6306D2388B41B9E" modified="2022-10-12" version="1">MDQTPSTIDISEIAQDEEQQYQSYIRQESTMEKKLQYSISANNQIKGINLQDIYNKEGWLMKKSPKLLVGWQKRYVKIYDGKMQYFKGLDKFKGCIDFHLISVSITPIMKNNEIHEIRLELKGIKKIFQFKSIERKELDDWYRCIQQHILITQFYPKITPLNNESMWRFERISETYFRKKADTGDILLFRGQSSLSKIQRAFTGDNFDHVALLLRYNNGELFLFESMGQTGVSILSWDTFMRNNWHTLYQQMVYRQLEVNRSNEFLEKLEQFVKGSIGKRYQMSPSKLIKRFTKSSESIDDLEKDDKTFFCSELIAAAYKKMGIIDANRCAASYWPGIFAQNSKLQLTKGNLKQEQLIDFSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQ85</accession>
    <name>A0A8S1LQ85_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0450269</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="MMIDLEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="314"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="686"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="1183"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="1513"/>
            <end position="1635"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="738"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="1565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8070468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1654" mass="193237" checksum="161A55537F8A7156" modified="2022-10-12" version="1">MTQSSVRNQNETMFFISLINLIFGIKKLEMFTIISNSILLGLILIYLLYKYKSHRKQLIFEQVIIVYYCCFQLVLRGVPSIWFNIFSISMDQHLVQFVTLIIVFAFTCAQVQPMVEISILQTILEQLILIIMFLYRKYNFTNLKKQTKNKILKQIIKNVKYYTFDQNQNELNGEKLISSEQIQNSNATLFSSRSENQKLQSQIKKDIKIKCYTKQQIEMVQESQIMTSKPSVFDTLSGFLESYQKDKQQMKNNVILASEFEELTGQKQQYRLYIEQFKQIQKVYTLVALVSLEDKIPSKQNKYIDKFKISLSKIFTHKLKTPLNTTLGFLQAIVQENNTIDEQVKTRFLKPALINSKIQYYQVQDILDYVNSAKLVTYKMSKVNLHKTLQLIYDMIELQCRAKQIQVIFTVNSKPFDVNDRPIYLTTDQLRLERILFNLLNKSYRHTQIKGKIQLNVDVLTENNQVQFKINDNVQGFKQEQFEQINNFARLQNQCIHGFRNSIVKRSTFKFSLTLQITNKLIYFLSDFQCSLQINHDLEEGASYQFFISLDCKQVNVQQSFEISQRNLSFQRKSSGSLKQHLPNHISLFTTNKNQQQDKVLEEYDIDEEPLANKPLQLSTQMAQTSPKSTLFKNHPVQQSILPFHKSVFQKEINDKSLTISMAFDHSFNVKRVTDSIDIRQDMNQTIMLVDDEPFNHDTLKLMLKSLGFKNFISAYHGQQCIELVKKNNKSIKIIFMDLDMPIMGGIKATKILIQKMSDGEIDYIPIVACTAHDDKDTQQECLMAGMICIIAKPVFIRSLQETFLRINEIKVKSQNFIFGSKAISSRKACDYIYFYHKNRSDYNLIKRIKGYDYNFYQPKKQCNFIYFLLSNMIQQTIKFHLMLGFLIIPDIVKGILVSQISILICSTLLFTLQIIQLILIVKCKKYEDSTCFILFSYNVIQILHYGTQSIWLTFCLLQIYKTNKLQLQKYSKAVLVTLSIILFSFSYQGKIVYSEFQSIIQCVAFLLYELKQVDDNASIIEMAENEILQELKKQMIIKIFDSNYRDITPKNKIQLEINSYVQITQNTQMDEHLQFYIKCYQDQDFTSSPDQNSNKSQQLVFQDCYSLLEFINGQELIQSNEYNHYIASSVDTQKNIKQQFVVMHKSINQGNLQILSFLKIDNFMIKQTKKKFNQFKKSFTDLFAHKLKTPLNATLGFLSSAYNSIDVDNQIKVNFIKPAYINSKLQYFQISDLLEFLSTQTDKIIFQSSKIYMKQFLQQLSELIESQCINKRILFQVFVDQIPLQKIENFYIISDVIKLERVLYNILNFSYRHTPSGGQINLNINFNQERTEIYFSIDGSGDGFSDEDIENINLWIKQQCDFQFKKQHLVIKKELKMSLKITNNLINILSSQEQHLEVAQSEIGVEFRFKLNIKESKTVESQQDIQSVGVMLRQRSLKNMKSSQNNCGTNKSIFSSKDTNIQLNTLNMIDEPQANQPLTNKIHNNKWQRKNKNQKSLCSSLINKGLIDTDKLILIVDDEPFNHDTLILLMKSMGYKNFLKAFNGQQAVEMAQKNQQNILLILMDLDMPIMGGIEATKILVQMMIDLEMTYVPIIGCTAHDDRETLDLCIEVGMLHVVQKPVFVRTLREAFQHIIHNEDFKKKSQYFLNSQLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LRS2</accession>
    <name>A0A8S1LRS2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0450177</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LRS2"/>
    <dbReference type="OMA" id="YKQNGPI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000009">
        <property type="entry name" value="stromal membrane-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF1">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="6">
        <location>
            <begin position="12"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8070337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="320" mass="37576" checksum="09B29165FA624498" modified="2022-10-12" version="1">MNKQHIPAKAQEKIFGLILKRPDNLVCADCATKGPRWVSLDYGIFICMDCAGAHRTLGPSVTRVRSTNIDGWYQENIDIMESIGNATANSYWENTMPKDYVKPTINTGLDSLIRFVQEKYIKKRFIPQQQCLDPKQQYMLTKTTVKPFYFRIETKQEQPKVKLGDLIDLNEQFDNFCVKDTKIEIVHGSNTQYGTTHSLSPEKDNTDFNQTKQQVNHVMHSLPQLDILNLYKPDQQQKQQMQSQQTIPQKNANYAYLQNLGQQQQQYNYQQYQNNNTNFNQQQQQFQQNLFLQQQTNQIQQQQQQYKQNGPINIMELYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LT74</accession>
    <name>A0A8S1LT74_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0430312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LT74"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="136"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="301" mass="36792" checksum="A5F9B9F37CA10942" modified="2022-10-12" version="1">MFYKIIYRSIKEQQNTQYLNQVQKNKLFSQKKRFRVDSEGISRIIIEESSSITLIERIMKIIGDISIDESIKNVADEKLNQSQLIIEQEKYENVQQQLRIVEKNNNCNKIEYFKNFIIQIDKVVRLLKNSIIKIYKFQLLNNLQEKQEELKRIDKKNDEVKRNYKSHQLNNQQYEIDVKDQYKIEKSVNNFLMKNVKQIYQSSLIQQKQQLTQLFQEVKQFRNIFLLLQDYNKEIQRKTTKVFLNYLQEQINKFSQSLWTTSNIQQIHNESFKEILKKYKSVYYKFKMKKEQQYIRINKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUI8</accession>
    <name>A0A8S1LUI8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0460195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUI8"/>
    <dbReference type="OMA" id="DERMKEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38150">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38150:SF1">
        <property type="entry name" value="PFU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="598"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="598"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="614"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8070899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="714" mass="83368" checksum="F47763B965BF0737" modified="2022-10-12" version="1">MSVMSQNNDPSIKQRDNNRQQITSLNQLSNMYGKSKPGELAQSLVYGDKPKKENNRPSKQMQTTVSEKPNQWQTRTVAVSPSKFTVLSQQLYNHIPNTLEAQKSQRAFHVKDQKLLLSPERLHSLPNQNGDKTPEVCNRLIEYKTFVSDKKQTLKDQLDAEYLSQCSFQPQIDEKSRKMAIKATNQEVRSADEFHEQQMRHLQKVNEKRDILQKQQEEDEKAELSSPKLTVKTQKLTLKYCEQPLVERLHEVKSKTQTLPDSTLGHSFKPQISKKSKEIKRDESVQDFLYNDAKNRLEQKKQTETMIPQSVIQPTDKSQSWFVQRFIKEFYYQLSGLDRVQETKDYSKIYQDDFQKILEGLGFGQCDMLWNELNNGFYVLSRNLLVILLAIQAVPQHLIEIPLYKPKVEENIQVKSKYQCDENGNLILSVSDCQELHKMFYKLYLNTKQQNGNGVRRRMARSPQPEIRCGSPKISDRTKQLAEAKRQDSSLFDWFSQQEEKKKQSLEELKKQVEEERMKECKSIQREITIDLHQLAKPLQKQEDRTTVDVEFDNQQQHCTFQPQISKPVQKTSASNYAVQEMEKQAARMKEARLRQKTLTNLKNKGTTTNNSIRQKEKCLTENELNNEPKQKKNKSRPISDKSTPMLYVDVKIEDGKSARIVVFEGDTSVSLANKFAKEHKLDENMKEKLKDLLDQQINSYLMKIEEEQEQEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXP8</accession>
    <name>A0A8S1LXP8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0450159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXP8"/>
    <dbReference type="OMA" id="IINFIEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035919403" evidence="2">
        <location>
            <begin position="18"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="233"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8070301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="365" mass="40482" checksum="0AF3F0E43E86F9FD" modified="2022-10-12" version="1">MNKFITIALIALLSVQALDIDFALLLQTGSQSNDAVQAVYDLLNDLKTSNIEAQGLADEKNISDEEIGQARIAALSKVNELNQKAWASAKARREQIGIEYREATDYIAWATQRLADIDRREVELEELRCFSNGLFVRAIKQHNDALGVIRILKNDLSGYLTGAPSSMVELKVENVADKLKQYSQLFNQDAMSKFAQLASEQASGNAELHALGENESTNGGDRQPGLNVGQLVYNALSDLEDQLKSALSALEQNEIAGYYQLADWLADSEAEKAHLQDEIQRKTQLQDKLVVQEQAALAVQAKANSVLKDSQNAINAATASLQELRDLYDTELNRRNEENAIIDEVIHIFKQQVLEMANQTSYGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYG6</accession>
    <name>A0A8S1LYG6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0460259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8071025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYG6"/>
    <dbReference type="OMA" id="WRYFRNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF3">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="23"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="311" mass="36499" checksum="3DE26AF38761FF29" modified="2022-10-12" version="1">MILSRGLFKLPLKQVQYFGKRKDYYAILGIQKNATEEQIREAYTKKAQKLYPNVTTSVAINDASAQQEFQDVAEAFAILSQIQSRNAYDLLSKEQPELLYGAEMERYKQSFERNDDGTYKRPGQVASDYAKEKQDYLKKERQVFNVDDFGRYKGGVPRPNKGSVRGNALMGVGQYHRPLYHNMKKNPFETEHRITSEDAEYFRVWSTEERTFKEWTYLHQNAVVDYDYLKFNDYRAFYRWTRNFFLIFLAFPYFFQSLFRGQLREIIDEINVEMAEGIPIVGRQFGNVQVVVGKTGTLKLQDANGGHHHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYS1</accession>
    <name>A0A8S1LYS1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0410169</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYS1"/>
    <dbReference type="OMA" id="DVRYEEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="7">
        <location>
            <begin position="64"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8067794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="153" mass="17366" checksum="7A6604CAB2C7AA19" modified="2022-10-12" version="1">MSHSTHSQLNESEIQKITYPHLADVRYEENTEKVFIGIPTQNHFIQSGIPIPTGFLITPVYNTDSNGTNLPFDAQGSIIPIQIQCKYCKKAGTTLMQYRAGPQTWIVAFFIFIFFLPLVFLPFVLKSTKDRVHYCPNCGQCVGKKKFKLCNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M1Q3</accession>
    <name>A0A8S1M1Q3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0510075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M1Q3"/>
    <dbReference type="OMA" id="GNACTCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8073489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="39180" checksum="3FCD70240436D95A" modified="2022-10-12" version="1">MGNACTCQCQTKPNDENEINTNQKQQSQSKLTAKPDNAQNQDPDGKEKISNQLKDEDVAQMAGARNNSIDDSSSPKLMKRENDDNKPEDQYIAQVQATKNKTTRTKLGAIEMKNGGVYEGEWKNAMRDGSGKYVWPDRSFYEGEWVEDKANGFGKLVHVDGDIYEGQWLDDMANGRGVYIHSGGARYEGEWKNDLQHGQGVEVWPDGAKYEGRYENGKKHGQGTLTFADGSYYKGDFVENDITGYGEYYWKDGKSYKGQWNNSKMNGKGVTQWADGKKYEGDYKDDKKHGFGIFQWENGRKYEGHWINGKQHGKGMITLPNGEKKEGIWENGKRIKWLNADESPNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M861</accession>
    <name>A0A8S1M861_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0470164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M861"/>
    <dbReference type="OMA" id="DQTDQEP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="774" mass="89601" checksum="CADB5D83332B8EC0" modified="2022-10-12" version="1">MFQTTSSINSQQSPNRFKFKSKVSFFNDNLIPTMTTKITLQYKEFLRNIISSIQEKVSFVNFPEFDQLKSQQIAVSIQSCYNPKFKSTGVDETKEAFQDLLDEIKQTINRKYPFIKVFRQQISHPDHYLQIQLYQPEVHSVVLYSRQFSKEQISPKLIADLVWLHLRKFNLTVNVDCPIKNVIPVCEDGLQRNILEGLQVTVRQREAPTLQSAQTPTKQIVIRTSFCQQGSAQKSASKLDIYKSTENIRDIEKSVRVYFSPTKSQAWMTQTNISQIDLSGQKQRAQSPEKKFTVKLFSQTVPLSCFDEAKFIDLVQQVNAYEIEITGNCIQTTKTQLNVDELLDDNTDTVYVSTRVERNDVIKCIVKAPEDATVNVTKNITETLTFDINEQAFIFYGRDKQEYIIDVSKKDCFSQKRTVFLQGNTDVKIEFDLVSCVETTLQFRAYNILLRSGQHKDIENCRLEIFQSDQTDQEPLIGMTNEQGLFTCQGLMFKQVRVHAQRKGFLQVSYDFDVLANSAGQLLNIPMIPDYYSLINQYHILIYVPNKNNFQLDFALVCPDGAKIDSKNRQHNVMKSKLEINRINQQTMLWNFSVHVQSLDPFLPDNCFQFFINNQKPKRTLLCQEPLSPQCKENGVSSAAKASCFKTVNLNEMFLKTRVMQTEIKTFVRQESDVIAQKINNYCAEESVRIFVTFGHKVLETFMIQSNLLMHNEKCFGVIDLDKRMFIRDNEVSQQNIKRNLSKMPTFKKDLKGGNEDRTMTTILSHITNQRFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M947</accession>
    <name>A0A8S1M947_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0520258</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M947"/>
    <dbReference type="OMA" id="TDFRFIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009082">
        <property type="term" value="P:branched-chain amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000017">
        <property type="entry name" value="Branched-chain amino acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000027">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825:SF44">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825">
        <property type="entry name" value="SUBGROUP IIII AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5 8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8074401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="390" mass="44329" checksum="DE238EE8CCA18AF4" modified="2022-10-12" version="1">MSVSKVQKLFFRFSYYKNLDLSSPYPSFQARDMTTTHTMHYITKPPCDPKIMTFGAFHTDHMLEVDWTEKMGWSRPQIIPFKSFSIHPFAACLHYAIECFEGAKAYKGPNKTIRTFRLDCNMYRMKQSAKRLSLPDFDGAELQKCIEGLLKVDQDWIPDRPGFSCYIRPTIIATEEALGVRASSRAKLFVVLCPVGPYFPSGLKPVRVFCNTTTIRSAPGGVGGYKVAGNYAPTVMPLKEVQQIGFHQNLWMLPDGLVQEMGVCNLFFFWTNKQGERELVTPQLDGTILPGIVRDSLLELTREMNQFKVIEKKLYIQEVIEAISEGRMIEMFGSGTAVSIQPIEAIGYNDKIYEVKYDPKLNAGELSHKLFDMITEIQTGGKPHKWINVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9I5</accession>
    <name>A0A8S1M9I5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0500291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9I5"/>
    <dbReference type="OMA" id="MRKDGPN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="139" mass="16469" checksum="01C12A557092486A" modified="2022-10-12" version="1">MKKDGPNISKVASDLILAYKQLISQQTEIVISPRYEKTALYFPQSPIEEQIEEENQLDSPRKIIIENEDDYLIEISELRKIVEQMRMKYMNIPEKYLSQPFKSTNQNQTSKPLNKNTKKHIVITKYQTDFLRQQYGKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBU4</accession>
    <name>A0A8S1MBU4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="18">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PPRIM_AZ9-3.1.T0540282</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Na(+)(in) + ATP + H2O = Na(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:14634"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="18"/>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBU4"/>
    <dbReference type="OMA" id="QLAVTFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008554">
        <property type="term" value="F:P-type sodium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000061">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000045">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000191">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 18" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0915">Sodium</keyword>
    <keyword evidence="15" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="343"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="797"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="831"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="874"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="913"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="953"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="985"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="21">
        <location>
            <begin position="48"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="19">
        <location>
            <begin position="161"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="20">
        <location>
            <begin position="822"/>
            <end position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAD8075591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1067" mass="118882" checksum="C307E127EE336F08" modified="2022-10-12" version="1">MKYQQQQDIESQRLSLLSVEDLTDLFILNKINDGQSMSKVKSFGDDYGLARKINSDIKQGISTEADVQKNRECFGDNTPVEKEPITLCELIMECLEDTMLRILLLAALVSTVIGIINEGLATGWTEGATIFFAIFLIISITAGNNYLKEKQFRQLRRRLDDGKCQVIRGNKVTEIATKEIVVGDILLFNLGDLFVVDGLMIQGSAVKMDESAMTGESDEIKKLPYQEMLQQKQQLLNQDASRGHTSPFLISGTKCLDGTGQMLVLAVGQNTISGQLKKLLIQDNPPTPLQQKLEGVASDIGKLGVIVSIFTFIALMGHLGYDIYLGEIQFKSLRTLQVLVESFMISVTIIVVAVPEGLPLAVTIALAYSVGKMKDEQNLVKNLSSCEIMGGANNICSDKTGTLTQNIMQVTALYVEKNTIKNDVHTIKSKLNKNTIELMCESICYNSNAFPQKDKVTNKWIQIGNKTECALLECADNFNYNFSEYRPSDKILRQIPFNSKRKKMSTAVYNPKTRFIRVYTKGASEIVLNQCIKMVGPNGVEQILDQKAKDQIYNDIIQAFASESLRTIAIAYRDLDPHSQNSSALGQIPQLTTFTQSIQEDDLDKDLVLVAIAGIKDPIRPDVPNSIKQCHASGVTVRMVTGDNIMTATAIAKECGILQSGKQLGQYEVVEGKTFREFVGGLKTIKDKDGNEIKEVGNKQNFKIIARDMKVMARASPEDKYILVTGLIAEGNVIAVTGDGTNDAPALKKADVGFAMGITGSDVAKDAADIILLDDNFSSIITAMKWGRNIYDCIRKFIQFQLTVNLVALFMSFLGAVVLKESPLNTIEMLWVNLIMDTFASLALATEPPNITVLERQPYKREDKIVSPTMNRTIVGGSIYQIAVLCGILFVLPKYMDLSMPEELVGQKFHKNVVQMSIFFQSFVVMQVFNSISCRQLDYKTINPFTNACNNPLFWGVQTFTLIIQCILIQFGGKFVKVSHLTVQQHLLCIAFGVGSIIFLVLVKIIIPEKFCQSFELFREDVITQDKMDDTLASRLRRKSTTRQRNRKTENSDYITVRSIQQSHQRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MGT7</accession>
    <name>A0A8S1MGT7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0570104</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GILT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MGT7"/>
    <dbReference type="OMA" id="ACMFRQD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004911">
        <property type="entry name" value="Interferon-induced_GILT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234">
        <property type="entry name" value="GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234:SF8">
        <property type="entry name" value="GAMMA-INTERFERON-INDUCIBLE LYSOSOMAL THIOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03227">
        <property type="entry name" value="GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035928931" evidence="6">
        <location>
            <begin position="20"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8076883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="198" mass="22301" checksum="9545FB328261648B" modified="2022-10-12" version="1">MINFTIIFITLTLIGNAQDLPNVKIYIESLCPDTAAMMRSISNSTIDSLDLLANVEFIPSGKMNTNTYEANTYMTNCQHSEEECYGNMILACGLQIQQDQVSSTKFVRCFFKEAYEQNFQKQIEKCLQNTQQKLQVKACANGPQGIELLYANRLRTPNLSYVPSIAINGQIQKLKEDFGKVLCSLSDAQAICKDIKWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MIA8</accession>
    <name>A0A8S1MIA8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0620246</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MIA8"/>
    <dbReference type="OMA" id="PKRFVQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041653">
        <property type="entry name" value="Importin_rep_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041389">
        <property type="entry name" value="Importin_rep_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18808">
        <property type="entry name" value="Importin_rep_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18829">
        <property type="entry name" value="Importin_rep_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TOG" evidence="9">
        <location>
            <begin position="356"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="774"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8079878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1079" mass="123942" checksum="CEECA6932B3F21FC" modified="2022-10-12" version="1">MNFQDQLQQIIQGIFSQNNQSRKQGEDQLAQLREAQPNEFVMQMLNLCRHEELKIRQFAPVYLRYSLSRFAPKSHKNVWNQLIVETKETVKLHLFQFIEVENSHIVRNQLCDTIGEIGGSLYENDNHNEWPNLLPTLWQMFLSSNNDIIECGFKILGNLFMYSIDQFDKYQQDLHTLFVQGLVSPQIKIKSSTMHALGNYIKYSLPTQYKIFQDLISNMMKSALEITIQDQSLGEGIMEVFSNIVDSKPKFLRKQFNIFFNGIYSMFRESQIENGVKRIGTETLLSMVEKFPGLFKFEKIYLMQVVEMIFFHMIQISSTITDEWMKPPEGFNDDIEQDEDCETTRFGMSSIDRLIESLGKKEMLPLLNPIVSELLRHQDWRCKHAAIMGLSQVGEYIDNIVDIKSTIELILPMLNDNNSMIRYAVCHAIGQIADDMKPKFQESYLHIVVPQFLNRLTVEDVPRVNSHILAALTNFVEGTEKGIEAYLPNLIQLSIKFLNTGISIEKENAISVIAATAESSKLFFIPYVNELLPLLFQIFSTHQTKQYRQLKGQAIETITLIASAVGEQVFLPFLEQTVQILIQVQTSNLEAIDPQKSYVLSGWQRLALVCPQQLAKYLGQIVPSLFQLIQQVFNINTKESNKELHTYDNEEAEVAIHMLSVFIEELKQSFFPFVEKCIQLIVPLSQFNSDENIRSAACKCLVSLVKNVKETNNSQQLINGAKYFLGIILEAAFKESDPSVIIQQIDCIKEIIDIVSQPFMTTEEVTELSDKVFKLLLESDKRRAENENLAKEEDVDEDEKNAIKEDTETEENLHGKIAECIGSLFKTHKEFVLPLYEIICNQILQKVLDQHKSAKMHQFGLCLIDYMIEFLGFPYIQNHFIDLAQALTIYAVDKICSVRQVAVYGIGLMAINTPQELYLQVSQSLLKALVDSLKAQKDQDDNEKHFGQARDHSISALGKIIKSQPQSLGQDLIQGIETWLYLLPLHYDKKQAYIQHNLLAEFIIQNGEQFVNGKSDNALQILKVFANCYKSKFSTTFIDSQIVNALRIFEQLDSVKVFLQQIFQKLSPEDQKKLLDIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MIZ7</accession>
    <name>A0A8S1MIZ7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoglycerate mutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0630243</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MIZ7"/>
    <dbReference type="OMA" id="MEYLWNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF61">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="30273" checksum="77FF116E5406DA3A" modified="2022-10-12" version="1">MNKSKFILIRHAESEYNLAARLAVNSSKEVTNFKEESLKIKQDESLIDCGLTQYGIQQCLESAQKMSSFNVDIVLVSPLRRAIQTAHYLFRDHPNKPKFIVVPFLREMLSSSCDIGGNLTTTMNEFPQFDYSRILNTEFLQQYPNMWTMEYLWNQEQKQQMKQYLLQQQCDDLNSRGPKHILDYLFQYPPDSVVETFMDTMNRIKQAKQELSFYRNQNVICVTHSRFLQTISAQQFDAEGKPKDGTWIKNCDYIEVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJM2</accession>
    <name>A0A8S1MJM2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0630252</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJM2"/>
    <dbReference type="OMA" id="FEQNIFT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="32824" checksum="6B2E97E447A7F27F" modified="2022-10-12" version="1">MQITYVGEYINGKKCGLWDVFEQNIFTGGGLYDANGLKHRKWIDLSDNFKDYIHIVYIGEYINGKKCGMWETLFRYDKENNFEKIFSGQFNDQGQKNGKWIELSDNFDYTCQVIYQGKYQNGIQIDRWDSMYRLDDDRGFIYIGEGFFDEKGQKYGKWIELWDNFKDESQVIFKGEYNNNKKFGHWQTMFRYSCNNSFEIIGGGHYNNDGLKQGRWIDLSECFSLDHQVIRQGEYKFGKKCNCWVVMKREREKKNDVFQIMDSKNQQIYSDQNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTI8</accession>
    <name>A0A8S1MTI8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0630084</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM U1 A/B'' family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTI8"/>
    <dbReference type="OMA" id="FKEITCA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030532">
        <property type="term" value="C:small nuclear ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12246">
        <property type="entry name" value="RRM1_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12247">
        <property type="entry name" value="RRM2_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000039">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000029">
        <property type="entry name" value="U2 small nuclear ribonucleoprotein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="20"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="156"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8080085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="26538" checksum="3FB0E1675D46E237" modified="2022-10-12" version="1">MNQPYLHPANQIIQDSAPKETLYVTGLNDKIKLEDLKFVLYILFSQFGEVLQMVMKKTQKLRGQAFIVFQNITYATNAKAALSGMIVYDKPLIIEFAYKKSVIFDRIEGKFYYKQKQHKELQPNLPNELMKEKKQKKFEEKIQNNNSNLNQGEINNVLLIESLPPFVTEIMLSELFRQYPGYSLIKLIPARGLAFVEYQNDDQATVALKGLSNFKITPECQLKVKYAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWE7</accession>
    <name>A0A8S1MWE7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0720156</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KEYDNWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037869">
        <property type="entry name" value="Spp1/CFP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174:SF1">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="248"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1597"/>
            <end position="1624"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1276"/>
            <end position="1303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8084580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1646" mass="195072" checksum="CD52AEABBC751D96" modified="2022-10-12" version="1">MNCSVCGLNFERKDKGCQQMECQQCKLKYHRFCYGYNNLDGVCDPCQDIAKKPLCCICGQKGLLKRLSDQSGVYVHVSCAIFAPHIQVINYHTMTFATKSQIDKKTKEKCTNCGKSGANIQCLDCQAYAHPHCIFVEQVEKAQEDIETEQWIFHLKFQANNQDPNQLDVESGEIKCELKEIQDAFYNALDKFQDKQKKRGNQTQQYKEHVKNTLESIFDNYTLQQCNQQWSSQNKIESYCQVHMESHRLFCICRKSLNNKQMVQCDYCYEWYHFGCIKRKNVKDDSYICEACKGWSAKRTKIDLEDPKLLNFEDLVVPKSVYILHLIDLLPLLLYIEAIMKRLPSIPLDENDYRNIKFYKLFLASLPIKPSTVIHLDKILLKEKLQEELKQKMHSIIPTQLEQSELFAEELVKSFKFKGYYFELSENKLIKSFVLSRKDIKVQIQKGISEGYLMEDTFKILNENITDNQKIFKSPNDNLGQLINRYKVAQQIKTTLRSLFEVERMQIEYPIIDNQLDLTVYKQLILIKNQKNKPNKMKLIDLKRLANHNNVTLGCLKIIDKLLEELNSLEQENVQNSYKKIIEFPVNSDNLINQLQSYFEQEFIDEFKVELELKIESFSRLTEKEYSGVYTYENIQKAAKDSIVIRSVYNQLQEIHQKCQTQKKVTIQFCHEILDLMQKCQLTTMYIENQKTKFLKFRELYKKLNESITLEQLDEIEHECFQLGFDMDVEQRRQQLLQAQEIIQNIQPSLCDSLDEFKKLKSLELDTYIKQTEQQIDFVKQLYYLVYNSYDTLPKMIQKVKDITDLDFNNELLYKVTIPEVVYDEMKQVVQNFRQIKWRCECQQILDQSNQMMIQGGNKKYNISQIIHLLNEDSSIQDSFYTQQLQKIKKEYDNWIINLQEFENQLTGQVAPHFNQLIKLINKNQYQDNNLQTKLWQYYIQMLWMQKAAEFIEAKANPQAYKTLISCATYANIDQNNVLLMKLKEHLFIMDDLGRKLKEYQEQRFLWVKSREQINNLQSYKSYYKYLENKPDAEQINQIYIQAKEYPIFKVKDLAEDLQEVRQVLQQYNDLVQKQPDNSLYIQQLQKIRTYYCCCFLKVKNFCVQLMYNMIKQQSLKCLKQLNPTEQILQRLEEINLLVALGGEEWFQKLSLTFKVKQENTNNSDFKFIDCSLSDDFEWIRWMEESSQNIGETFGIETKKQQKEQKIKKKKQQNQQVLEDITEDMRQTQRLDLQVCFKKCSNWKQVNEQQIRSLEAQVFMEQSFDKEKYLKEIEKIKVVIKQNKQIELNYEEMKKAYKQYLHQKKSSSQHTSSKQALQLESVKVIKKQQPQVPQITSAQKLISQHSIKSEPDVEKKVKPEIPKQPTIIAQPIQIANDVLLYNPDGQIDTPCKKQKNQLMNIGEINLQIKKKVNDTRESFLIKPQLLTYEHHMAQHFPKTENLTITCDTFSPVKEVMEYFTQRKSGQFKMMIGWLYSKDLNVAGDLFKLADKLKHSKQCVGTKQGDMGLTLIYYDYLNKLKPTTKWIIMKEELENQLIMDSLNKYHNLEKFKPHLCFVYYVKDFKECTNTILPQPVQYINVLKHIQFKRVIENHKLQQKKQQGQLIPSKKKELEPISDDENNNGFQQKQDVHSMLMEIPGILSLLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXV6</accession>
    <name>A0A8S1MXV6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0670173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXV6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="62"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="152" mass="18071" checksum="013EBF24F8BDA0C9" modified="2022-10-12" version="1">MKQKKQPQTPEQIIYILRSFKSYIGKKNSISPQTRCQTCVQTVKKKAKQTFDCIVNQSIGKMQDQEEGKESVEEKIELLSHQIKNMYIKQEQLESQHYQLSRNVKKLNLENKHQVQERSQMIKKLDYMINIQTKQEDNLNQLKQLFASRKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MYV6</accession>
    <name>A0A8S1MYV6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0740196</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MYV6"/>
    <dbReference type="OMA" id="TWKYKDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002041">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="7"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="384"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="570"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="528"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="396"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="455"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="465"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8085558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="760" mass="88433" checksum="5178DDCCF04A8D48" modified="2022-10-12" version="1">MTELGNYLIGKTIGEGTFGKVCHAKHQVLGHDVAVKILEKKRINDELDIERVKREITILQMLHHPNVVQLYEMIETDTHIYLFMEYADGGELFDYIDLKKRINEVEACKFLHEIISAIQYIHQLRIVHRDLKPENLLLTAQKNILVVDFGLSNTYEDTLKTACGSPCYAAPEMIQGKPYYGLQTDLWSCGVILYAMLCGYLPFEDNNTQVLYKKILTADFHIPRYVSLDGKDLIKNILAVDPTKRFTIEQIKQHKWWQLWKSDNTQMTPFKASFNKISCTLLPNSIYKSCVKKNIERNISEDVKTCCDTAQQSIDIQFIDRDDFINKLNIKDEIKQNPPLQDQTEEDLNQKFVNVEDQYEFQQSMRSLTRQKSSQSIQSITHLHTQIDSPIKKSNLPITTKPTFKQQNSQINKVIPKKPVLAQQQQQSLQQKKPVTKTGPSKSLHEPPNFESKITKQPNTPSTKQQQNNASNSQNPSNNFSILNHQLKTDIPKKNSIPPVKLTVIQEQIKENKEKSHSLKTQPTQTKQQQIQQQSQQQQQQQQQQQQQQQQQQQQQIKQQLNLNKPQNLYKTPIKQNTPQVSQTKRSLHQKQAASLQMERQSFHTQQSAVEENVTFQFNDNPKPSIENTKLMVEKFTSRLNNVTIPSSLKQPQKQVVQDVKGIENYYGPFNLSFITTKHPQLFLNGIKKYLEEKYQFIQIQNYQNGFTFNLNEQNGDLKLYRIENLEIYYCQINCLNSKNIANYSEFNTIIYDLQNNFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZ81</accession>
    <name>A0A8S1MZ81_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ammonium transporter AmtB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0740213</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZ81"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000008">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF6">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="271"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="21"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8085590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="432" mass="46011" checksum="D61ABA29DDBA885D" modified="2022-10-12" version="1">MNHLSEASTGTPATGNIDSTWVLITSFSALLTILGFAFISSGAVRYKSVQSAVITILLGAVITILFFWLLGYGFAFGDDKGNKFIGLNRFAGAGFGADQTKDDYTNLIFQSVGAIIVSSLFGLGTLERSRFFSISVCLAVISGFLYPTVLHWVQPTGWLNKFGFIDFAGSSYIHLFGGITALVVSIFLKERRDQAGNVHPGIFPHHSPINIGYGSIILCIATLIFINGANQEGKTDKYEQGLIAVNTLIAATFSALTSFLAQYLKTHKTSLIAISRGSVAGIVAISAIANDVRIWESALTGILAGLVYIVLILVIKRSHVDDPAYTLATHLGPGLLGTLLVGVLSISHGFVTGNGFKQFGLQLVGALALIGWGLLIALFVVPLKGTGVFKINPLQESKGIDTSYAEGEAIQFVDEQPEPALLSSTPKKGIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N183</accession>
    <name>A0A8S1N183_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0760111</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N183"/>
    <dbReference type="OMA" id="HRYQIAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8086310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="595" mass="71325" checksum="4ACC49381A68CDFF" modified="2022-10-12" version="1">MIKRLLPLSKQIISTVKLSTNMVYIQKIQIPNQQNINKSLYNFCQNNNISIKEINDHIDSIQQQIKAHYDSEEVFNNQLQVVQYLEDNLQNCYDNYLDYYYIQEILILHRYQIANYAFINPQNPLNNALTQKLINNDFNKYIDKIKQNKYLEPENLEYLKIKLKAASIMCSMINYGMEADINQLHDWMNDYLDQLEIHDQICSEILSKYDFLTTDKMKFIDLNDIHINFFFFIAQIHRKRNLMDEFERFFDQGIQELESRADQILKMPEGQFDLHLILNNLFQLFANLKSAEDIQTEIKNNLVLKLIDLFSTYWKDDCFLDFMVSFADYLVSQDLFEKAAELLKEAYDYKADIVSEVMLFLLYANIYQCKKLGFLKEIDMNNFLAQIEKLNNIDFEDMIIYVFYFHKNIFNEILETKNLEKIKEQFDKVIFYIQEMSRVMKNNKLEAQLPFFDWIDSVMTNNLLKNNDYYILAIYNTLNLFKDIELIQDRQNLERDFYLIVLSFCLKDQINLDSQSKVNNVKAFAMKYINNQEMQDIALNKLLLLNFKLIDDGYQKECIEIIDQLIIMIPKENDKIYKFVQQFQNQKNQILLKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1H3</accession>
    <name>A0A8S1N1H3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0640233</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1H3"/>
    <dbReference type="OMA" id="YLRILWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="388"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="245"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="279"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8080924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="51521" checksum="3DAFF4E8A52519B1" modified="2022-10-12" version="1">MNQKFQQKQDFKCKDHPQTSAIFWCKSIDCRENRIFCINCQKQQKHYQHFIDDVDDINQLNQFLITKSKQSKDLITECQLQYKSTVQSYEQLISGLNYKFCIQEDKINEFQPNQALTALDSLVLFDDMKIHIKSNIIGPLQKIQKILEDLYIQLELQTIKYQISDQQRATNQIEYQKGSFFYIYFFQLSLQIQQINQMMQFKYLINYYQLIPLIQNLSNSLNKLNKQQEAQQIYTNIIKINPQYELSYWKIGDILREQKSYQKAKDYYDKCIQINNNNSASQFGIGECLKETYKYNQALEYYIKAIQLSQSNQLYIQSYGDCLRELERYQEAIQAYDQILNNQSKNADASAGKGQSFNNKLRNMFIVFKHVQRSRGFIKKLINSQIRLYLRILWIWIVFIFIRSILRCNKLFCQSFGYQSIAYSFIKWKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2K6</accession>
    <name>A0A8S1N2K6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0650103</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2K6"/>
    <dbReference type="OMA" id="ISFNGQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="88"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8081204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="507" mass="59506" checksum="9687BBDEDFB22313" modified="2022-10-12" version="1">MGLCSSNKNQKPIQQSQLILSQPDQTNFKQSETQLTKQTKTAIPSIMAFQIPNLIKEDIDPPTPQSFRHSFNIIPEIKKSLEFKTGFFKTYSVITKNQDDNTLICQHNKTAELVLVYCMDPNNIQDQQLIKSIKLNQNDNNNICLFIEIFNENKTLYLVQEYCKGNKLSKLLNLERSKIIFIMSQLIQVLNQLNKHNLYHGKLTIDSFSLVDDQSYYVKLTDIKPIFQQNKNLETSKEMDEQNTYKRYQPPSEHPSIKSDIFAMGIIFHQLLTEKLPEKIKIINNQPIWNPLLKFQNENDEKMKKLLKSMLQLDHVKRVSVQQLTQNKILSILNNQDLIQEILQKISICPSLNYFQTAILTFMIQKFQSQEFKIIKQLYQILIENNENYLQENQLEQICRNSNLNKVEFEIEHHETYNNNNPLQNNSKRISEKDFLIALSNRDKLLTEENLETTYNQLKNSNGLLSAKSINRKIYIDQKQLLDDMEKISFNGQITINEFKDIMQLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N3B9</accession>
    <name>A0A8S1N3B9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0760183</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N3B9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000210">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000048">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1059"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="14">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="214"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="13">
        <location>
            <begin position="472"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8086422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1114" mass="129622" checksum="AF39DB27B2BB8040" modified="2022-10-12" version="1">MNSQSLSVSGISDKAQIINNKEILEQFENGEKIMLSTLIIKVNQNQKKQERIFLITNKYVYNIIPKSDSRIMQIFGKIMSNQIKRKIKLSSIQAVSVTSLGAEFVIHVPSEYDYRFQSADYRKQILETLCLCYQTVNQRKLPFFFKDDFTLVSVCTTENDVKKGIKRFPTEKPIELDIKELREHSNRKPQEQLIYKRPGLQKQISSTIISIEDFDLIKVLGRGAFGKVMMCEKKDSKELYAIKSLRKEHIMDKNQIEHTRAERKLLEEIENPFLISLEYAFQTEEKLFFVMRFMRGGELFKHLRDKRRFPEQTAQFYAASILLALEYLHKMQVVYRDLKPENILMDEFGYIKMTDYGLAKFLKPGDFTYSFVGTPEYLAPEIIRQNGHGLGVDWWSFGILIYEMLVGRPPFFSQNQSQLFKSIVESDVVFPSQLTLSNQVKDLIVQLLIKNPFERLGHNGDAQQIKEHPWFREYPFQDLIDKKLQAPIVPKLYDKLDVQNFDQEFTREEAMNSVVNMDPKLIEKFKQDFGGVTYVPNNNAEVLNLTSKTLTIQDSYGGIIEVIQGSEYRLKIFENAEYAINIELKKNSSSPKDICFSKQSTIQFGEYESHHKVIYNSTLNPDEEHANDPSYYLSSPIMINMVHTKQGLVIVTSDHTLLNYKVSQNYSSKTGFSTQLLSIIDFKNLRKHTTELEVPQIIYVPFNEYIYIIYQDQIIGGKIDKNIELAIIPFSGFQNQNSYINQIKVYQNFLFVPSGQDGLNIYKFEESGNINYHSTLSSSNLFFKIVPIHLIDIVFSISNTTYAYILDQLNGVSRFQVKKDGINVELIRDDQFGLIPIKNAMTLAVSPDEFVLVLKQVGVFQQLIEIGFINSDWFFLKTHHLTGQYFDIDIGPTFVLLRGKDEHRIIRVGIYEEFEPDYIFYTQADYYTEKANSFHEQYVFIPKLQAVEFYNDNFAIGNDPSKWSFNQSYPFILGLTQHTIVELPYISQPPYVYCSPDSNQDVGIVYKYDINLYSTSCPEKDKYLEQNPNIPYQTVQCLYQDSFNVKAIQAKGKLYDTQFQVIIIICLILFLTVLISISIFLYRKYHKQEESLTRSIQGFENNREYDFEPNEAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4L2</accession>
    <name>A0A8S1N4L2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0800185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4L2"/>
    <dbReference type="OMA" id="MEKNCIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="264"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="350"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="607" mass="71735" checksum="F52E3DF56C062F63" modified="2022-10-12" version="1">MIKYCQFHQWEEEKYICIHKTRLQKEDNKFSCHKCLDWKKSNNYIKSMKEVEEEAKQNIVKFVEMQTKIINSNNEMKQKYLESLTELCQKINQYFQKQQKSVTSLVEQQNQILTQNINQANSLLNEQSVLDSQSIDNFIIFTTLNEANQKIIENNYNNINKCLERQFNYEFAEIQCKVNQFTNLEFKFDSIPKYEVEQFKILTVDKEKDKLQYCKDHQMEKNCICTHEDCLKEKKKEYLCLGCSMNSHSQHFKGNFIKTVKKIKTEQNEKLKIIKEQYEMLKQEVENRIDKELTTNQQKQQENFVNSQTTRENLKQLEAQIKINPFNQKPNYTYYNIDQNLLSYDQDMIKLEFQSKLDQLEQLKQQITKQEKKSLINNTKLLKSNHQLIMQQYEIQISELQNKLEANSSESIIAEIKKSQKLLIEESLKQSQKQFIEGVNEIKQQIQTFPKVVTESKQFSSLQSDLNNVITIINKINQQSLQIPNSHQELKNSINGQEGIKQQITNLSNLNQDLKVMFKGMNEIKSSILNIPKLVLESKQFSSLQSNTTDMINRIIQLTIKVSTPQIVYQPQQILNNQQGLNEQPMGAGSFQQNQGFHYYQQNNSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6N6</accession>
    <name>A0A8S1N6N6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0740170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6N6"/>
    <dbReference type="OMA" id="SISVYHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8085506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="966" mass="112605" checksum="963EED3AFE04EC5E" modified="2022-10-12" version="1">MMNQQEYTNLIVLAFQGKETQARKKAEDQLIQNCQNDARSIDILCELSSQQNDLLLAESAAITIITAVKKFIANTKPYALEMRLHHVDLFVQMLTKQISDKIKVSIQQALQQLVYYDKQNHFKTRLKQHVIQSIETRLENMIASAFYITETLQLDNQYEWFQSIIKAGKLISTFSQSTKILWAKVLFHQLDQHLTTHQMTGVPEPIQQYFIYNEELCQVLILKTQQLTFVGDDLEYCHLIVASQRLIYGSIIKFYKEAIIAKKCPFLDILSILCDSYIQSLVAYVMSSNFSYDKLNQNQQNLINEIIKLLAITCKSISVYHIFAEYRMAIFLDIILPFFSSTQKEMNDLIEDPNEFVQLTQDLLDEQKSDIIKSSVAQLIIAYCQHIDGSVSFFTSFSTLAATYCIQKLSNAEIKEQMGLIMEFKEHYFLKSMNENVLLETSLLILSILSNYLVYRNEVGQIFKSFISSYGDYLLNYSIPLVKARLCTYIGAYCKSILHEQEELSQQLLNFLINQIKSAKNELYANSYCAIEAIKNIIEEPNLEKLLEPNIGQILIALCESLLQSDFEDHFDTIKQIFKTFTIEPSILDQALGLIVLKIQQEQQLVEQGQTERQICINQTWNILKSLPEIDKIIPIHFNLLEKRVAILYKYIINPNIIDFDEDIVYFIAQLISKTKFISDYQAEMFFQCNKVIEKQRFTLGQLFELFNYYIYYGKTLFSNINAQEFLIQMLEVVFNNPQNGEASQGEAIILLHLLLQEYTLNKDVLTYIYTKILQRSQLEVKNDFLRARLMGIYISGFIQNCSQTLAWIEQQQGFIYDHIIDSSKHCQPDYDSQLYIVGFCQLLLKNPKYLNVALLTNFVTVLKRQYNHDQKKDKDDNNDSEDMFAFNDELEDSKITMEAFQCNITKHNQFDMFHLTYQHLRRTLNIPELISNDPILKKDLDEMLKINKINSDARIILKLKKRKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NB38</accession>
    <name>A0A8S1NB38_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0830140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NB38"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="106" mass="12836" checksum="9412E0C7B5257F1B" modified="2022-10-12" version="1">MNVPEEYSQIIYRLDDQFNQIHKSKRFFKSQRMDNFTFLSANISKFTNSTESSNLPSEVETARYLSISYLFYLHSFYQLNKDLYPNKKIQNLNIILNFRTIFSYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEB8</accession>
    <name>A0A8S1NEB8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0780208</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEB8"/>
    <dbReference type="OMA" id="VYEMLMS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052671">
        <property type="entry name" value="Acrosomal_SP-10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17571:SF34">
        <property type="entry name" value="ACROSOMAL PROTEIN SP-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17571">
        <property type="entry name" value="URINARY PROTEIN RUP /ACROSOMAL PROTEIN SP-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="115"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="292"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8087455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="471" mass="57531" checksum="037FA5D114F08869" modified="2022-10-12" version="1">MNIAERLQKEAEKKRLQQEEQLKIGNKAIKEKYETEYQENKNKLSQEIKQDIKLISQQYPSFQKFALYKIYKQFDFNREIANEYISIQIQNQNQQDNDDDWIEITSKRKTLFYKMSQSNKNNQKPKRSQSVTNKKEPENDSQRQSYVNENQRNQRRNISGDRKREVSPRKFNYQNRKYEKFDNDRNNIKNEGRRKQFLNQKPNIPLQNTYQPKYIYVPKYEDDECPYVPKSIVTQTQNVFHQKQQEQNLNGNTQETINQQSNTDLFKQVMVSQQQQNKQDCIVQDKITQDQLKKQEEQIKQDQIQKEKQRLEQIKQEQLKQEQQRLDQIKQEQIRQEQQRQNQLKQEQQRLEQIRQEQIKQEELRQEQIKQQIKNEQIRQENIRLEELKKEQIKQEQLILEQQKNSLNQTQQFNYQNQSLLQDQQQPQPLNQQFKSSMFAPQFQMVPQQENSNNNNQPSVYEMLMSYINYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFB1</accession>
    <name>A0A8S1NFB1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0850032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8089969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFB1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="109" mass="13170" checksum="AB2A3E1897122312" modified="2022-10-12" version="1">MKFIIQLYYFLNKKYNDGLNLNLLQIDERIKAYRYDSLETIKYYYISIRFKNLIILSILQLVYEFNCCINYSQQQLHVLFDFQEKALNRCAIQLEDGHNNADDMAQTFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHI0</accession>
    <name>A0A8S1NHI0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0820152</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NHI0"/>
    <dbReference type="OMA" id="ENQFNDE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052667">
        <property type="entry name" value="E3_ubiquitin-ligase_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47156:SF10">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM-21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47156">
        <property type="entry name" value="PROTEIN CBG20824"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="39"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="235"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8088943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="263" mass="30884" checksum="DA3BB3FB467DD96D" modified="2022-10-12" version="1">MKVVQNPTQYFFEQSNNPPTEYLSKINEFVSKLLNQRQCILCKNDYNMKDRLPRILIHCGHTICTACLTNFYRNRRVRCPLCLKLIKHLDSVDRLPINHTIFTRMADDINNKSKQHGGTEVIDPQQYLFTQFQQSALQSQKARQQQQNAYPQVDPDSGLEFCEFHNDRVKHFFCMKHKVTCCRVCSDMIHQKKDCIVVDLYEIEDVPAFLKEAYKLNEDNAQQQNQRREDMGFIQDGEANDGENQFNDEDLEDEFEGNSIQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJV7</accession>
    <name>A0A8S1NJV7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0770164</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJV7"/>
    <dbReference type="OMA" id="KSIACSH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="325"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8086924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="489" mass="56785" checksum="35B016D4A24559CE" modified="2022-10-12" version="1">MQNSSKKQESHDCNDCWSALLCGLVEILIKTKNQDQIKLLIPLANQFAPIRIHKTIELLNQMIRNPNITLFYALVNQEESSQIIKMHIFAQYKIIAPSICNKDILQKLGQTFKIDMIFLTYNTSISLSNSENLPLILKIVQFQSHYTVETQEKQRSRSNSLQHDFILEMKQECNGCLRSFDQIELNKSIACSHTYCKQCLKLYFKLGSYFICRDFYCKIELSKDDFPFLQLSPLMDPYSQVNKCLQCKLSSEKLYTNKCGHTHCEKCIKDSLNKSKYFQTHFCLEPSCTEILNNELIRNNNSKEIDVNQLLQKSNSIKQKASDFCDFCKKTQEQGVKNKCGHYYCKSCLQSQYQSIITYGLNKIVECPQCNSKFNIDTTLEEYYIQLQQLSTPRLNKMRSISQQQPDLQPSPSLGLILNPQDQGIRLKTNEDQQQQNSQLKRQNSIDLKSIYNGGSTPRRIIPQISTSSRNNYPQTQQSLQFHRSPQFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKP2</accession>
    <name>A0A8S1NKP2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0870073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8090986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKP2"/>
    <dbReference type="OMA" id="TEDHNNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="280" mass="32873" checksum="EF2B91A9864209F9" modified="2022-10-12" version="1">MNQSQVNEENSPNNIYENASQNISQYLQRDYYEKFKLGQNLYDQSITLSRLSLGEKTSEDNNNNTQIGLIQIQERLSDEICKNQLLESKVTQLNKDLQSAMDHTIILQKQNENYILMLQQTNTQLQKQIRNYEIEKKINQQLMKKNRELQEVNNNHQKQVLTLQKNKQIQNFSDCKNIILSKCHQKSIQSICSSNRNKEKVNQINTEESTVKDSSFSELKSNDNHRFAESAQQFYKTSNLWSPIKYSSPKAQNFDLEKKIESTLSQIRAIKMQIQQLNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NL61</accession>
    <name>A0A8S1NL61_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0840109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NL61"/>
    <dbReference type="OMA" id="CYQENIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="108" mass="13314" checksum="61A555716D837D32" modified="2022-10-12" version="1">MKYTEKISYHVQEFQNLTKYYLTNYMFEQKEKCYQENIKSVDNYVNCALQLVNQFNEMSKKFRYQGLYFEHRFVDCLKHRPDEGDNYKCIQKLERDLKIEAKKITPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NMP7</accession>
    <name>A0A8S1NMP7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0880117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="304" mass="35478" checksum="6EFE215FC8AC9884" modified="2022-10-12" version="1">MKTNEKDEILLSKIQTDTVQKEKLNQQNQENHVIISILRSQQQTQMPSIQASQSDFFIRFNKKTLILNQKIIRSSKNNPYRILMVYYQNLINLKIIQNXYQNVACGKGRLIHADGDKYEGEWKNDKAHGYGKYVHMDGAQYVGYWEDDKQNGNGKEIWPDGACYEGQYKNGKKHGKGTFKWADGSIYTGDFDQNNIQGQGEYQWEDGRIYVGEWKNNKMDGKGVFTWLDGRKYEGEYKDDKKHGYGEFKWPDGRMYRGQWANGKQHGIGIYIGSSKIEKEGEWQEGKRIRWLKKGGQQTREEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NMT6</accession>
    <name>A0A8S1NMT6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0850216</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NMT6"/>
    <dbReference type="OMA" id="HQKFQDT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="10">
        <location>
            <begin position="3"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="84"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="123"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8090335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="269" mass="30581" checksum="E9606D4C8E227048" modified="2022-10-12" version="1">MLKKYSSLAQMQQESARSDRFLFAYRNNVYDLTHFVDDHPGGRFSLQTFKGKDLENILFNASIHRHQPSILSSLEQYKCGVIETKNPQDNKPKPQVIQTAKSNPPQKKEQICKTQLTDDKTNRSNVTSQSKVSIQQNSNLLSPDSNQVKKSNLKQTSKTKPINKIDSNIAESTKTIKSKSDLNILNHQKFQDTSQFKGSSSQISIISNPKISLLSPQIIHEEDESQPSPLNNVEKFESGLIPEDNINLKVPILNYQLTGYLRFKYTLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPH1</accession>
    <name>A0A8S1NPH1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0880085</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPH1"/>
    <dbReference type="OMA" id="MQQSYYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF271">
        <property type="entry name" value="MYB DNA BINDING PROTEIN_ TRANSCRIPTION FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="108"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="178"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="234"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="234"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="371"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="513"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8091473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="681" mass="81641" checksum="AEFAEB5960FAC7B1" modified="2022-10-12" version="1">MNQKQEKKIDDITLQQLRSMAENGAGDSLAASLNLDQEDLIIWPLEQVQRNFKEFMRSTSQIEIEMENAKERIDFPADMEWPTEDDLEQMFQKSMSKQSFLYNLCRIQGRQNKKKWKDNEKQFLTWIIIRYCLWKNKVCKELNDYDWRYIAQLIVGRNATQCKYKWLARSKFKLVQVPWSKEEDEALAQIYMEYQKQGKHSKWSEIAKEIALRCKTQIVRQGKQCRERWINKLDPQISKGPWTKEEELTLLQLILKKGKKWSEISKIMKNTRTENSLKNRYHTIMKKERNKQPDVKEEIQQEIVDLLQQHNHNYQADNLVHLYEDIDPKELKIILQVIDRLSESTGKQIRVSTLITDQQMQQEQLYEKQQKVKQESQIDQIQQKNEEANNKQRFLDTMEKIKIDLDQQIKKLVNKQELDSNCTINLLKQILKPQENETNQLQFDQYYDQMILNFQANQVEFEVEAITNLTAQDVEDCQFGIVDFEQSNIFMIPQQYLQEVINKVHGSSTQAQVQQQQQMLQHQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAQQQSQIQQSQIQQQQQLQQQQRLNISSSLRQSIPQSQQAQPLQTSQLFLGDQFNLVNSQYDFENQQQYQQQIPVQILYPQIIYQPIGQSINLSQGLQQNYWMQQSYYQPQYYMKAQEQQYNKSNQEEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQ09</accession>
    <name>A0A8S1NQ09_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0870188</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQ09"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8091215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="95" mass="11139" checksum="3DA43BD3E5CFA875" modified="2022-10-12" version="1">MNSEFQRIKFTFPVIEKFIIMDLNKKIALEDLKFTFTNILKIDLFAKKLTFMINKGFKQIELNSQQSLKGQIALENHKKEQAPIEVIVKFFEEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NSW3</accession>
    <name>A0A8S1NSW3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0920118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NSW3"/>
    <dbReference type="OMA" id="SIQIKCA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8093096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="317" mass="37299" checksum="D4CE7ED46194CFDC" modified="2022-10-12" version="1">MIMKIKQFLNDNTATQAINKVIMALSDLKLIVNDTQFMSQLEISNRLKQFFINDQTKMLFLNQFLQVVGDHLQQKENFYVKLKMLLLVHIIISSQVARADFSKMIINTKLTINIKANDGTDNLIGQLCQSYYCYIYKLASQTTLINEDFGKPQDDLMIYFTLSNQCYIGMNMQRLIETINNEQLPNDIIAHLVKFLYFDIQDIYIFILKDVKYLIEKNPNLINNKQQLLELYKECQSLQARMLTFYKFNRIFPHFNQIMPPHSIQIKCAILNLVIEDKQIQETSKFNAHKIREPLSVKNSEKKTQDYTRPFSPKVNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZD6</accession>
    <name>A0A8S1NZD6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1030001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8096999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZD6"/>
    <dbReference type="OMA" id="NIYKGAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="234" mass="27055" checksum="0DC19D588914348F" modified="2022-10-12" version="1">MNKINQILLRLLKIKKITEITEINKRLNQKESELQTIQKQFDQINQEKLQKEKQLKLDLEKIQQYPTTLQFSVTYKGVNCQVTEGGKVVECPGSSTYYCLCEQAIPNTGKILFAFQILSLTPNMLIGIGFRDIIQKNNYQNVGVGYGSYMIHQNGYTYSHHNKDVNNKQLSYTYTTNDIIIMEVSIEHKYIKWTQQNNPLTTVMEIDTSISQELYPCLLIYQSSEVKILGNIPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZK2</accession>
    <name>A0A8S1NZK2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0920077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZK2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8093014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="103" mass="12030" checksum="CC137FDD0A831AC0" modified="2022-10-12" version="1">MGGIQLLNIKILKMSSALLKIQSNLIKNYEMYKEFTIKQINQIQGIHIQFKQKIELCEKQVVSVNFLNLIKSITLIQDMPDQKQYQAQLLLNLLVILVKMQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P252</accession>
    <name>A0A8S1P252_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1030149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P252"/>
    <dbReference type="OMA" id="KESQTTY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="132" mass="15107" checksum="F49C5E1D6E53BA58" modified="2022-10-12" version="1">MDQLALKKLVNKLIDEKLKLVCEECRKKTTQKKLGYHNFNISIQPTPINFDFSIDNENKENCLSSNRNANRVKASKSQALDLGQQIFNADISVIRPISQFSSPTVKASRKKSMPFLKKESQTTYEKVLKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P4E3</accession>
    <name>A0A8S1P4E3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1050071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P4E3"/>
    <dbReference type="OMA" id="VGQQDYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38150">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38150:SF1">
        <property type="entry name" value="PFU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="47"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8097858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="462" mass="53888" checksum="F7E4E32508F46764" modified="2022-10-12" version="1">MINLSDREKELIEEICAKNTKSNKKINPDQGSQKLVSQSSTKILVSKLERKLNDTLKEIDEQNSGQINFQQLGRLFTLLDIFQTISYNQDYQLETQGLNNPMQKQQEVDLHEKAFAIISNDEEKVDIQAAFCLFRIILDPNQLEPSKCAILIQEYLEKISEKEFDYEAIYKFCSEFQAYQKTRLSGTKIGYLKASLAQNLIETYEKTLTFKPTINPISEALQQQSGKKENQESQSQISSGLAESRVNELYKKKELSKQKIEFLKQEKFMKDMQECTFKPQIISKKELPNVIDRLYQVKNRLEIEERAKIAEQEKEESEYSKCSFHPQINYSMPESQSTGIGGYWEAIERMRRANDRKHLKDLKLNHKPSGENYEKVKNQPFSPPEMLQRRQQKKELPILYIDIKLGPRKVGRLALRRNDDVEEVVKSFCKVWGVGQQDYELLVNQVKENLQSVIQETDESQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6U7</accession>
    <name>A0A8S1P6U7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0940203</name>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T1080016</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the histone H2A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8094067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6U7"/>
    <dbReference type="OMA" id="ANEMFIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000008">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="10">
        <location>
            <begin position="18"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="11">
        <location>
            <begin position="98"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8094067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8098809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="133" mass="14088" checksum="C9CA2DADB4054FE1" modified="2022-10-12" version="1">MSSGGKGKAGKGGKAAGAHKSVSRSHKAGLQFPVGRVSRYLKQGRYTERVGAGAPVYLSAVLEYLAAEVLELAGNAAKDNKKNRINPRHILLAIRNDDELNKLMANTTIADGGVLPSIHPQLYPSKEDPSQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8W9</accession>
    <name>A0A8S1P8W9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1100035</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8W9"/>
    <dbReference type="OMA" id="QWIPVIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035764901" evidence="3">
        <location>
            <begin position="16"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="382"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="615"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8099576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="678" mass="78799" checksum="11ED9752AC886FC9" modified="2022-10-12" version="1">MKLFIITILVLVVNGDIKQLDIKENLVCKQLILKGELKCQEVHADRIVTEEIDIPTEFSSDVLELNSLSPFRSDTRASAFIEEAREDTIFISANFQEIYKDDDDESTDYKTIQTDFIQIHGQSQWIPIIVDEQNIGWKKESKNLQNDHEKLRQILKQNSYFSYKHEHFKRSQLDKYKYERNDNNRIYGKWNDELIFQYNDLPQNHTYIQVTANFVMLTDFWRFGTRMIMRVDGQTVWMQSHHQNQKICLYLEHLLSSIQSNRNLQTEFNEMLQQEWTIPINLSVKHIRHKLHIEFGLILGNPDFHCLVLMILHYIINELNSRFQLIYIIQMKLIVLFALVLVTIAVNDNQTKQKSIEEQHPSLFRALKNMALLQEQSQQLDYSRLINAINDLESEITQNKEDENASFNKDFLSNNADQEFYENQITQYQVEVAQHEVDLADLTEARNTLQSLLNDAIKEQQDLTLLRNQLDQQIKTDAANTAAQLQEYDDSAAAIDEALNMLTSLKNTDSLVQVNDKLKTHLSKQKVFFQPLIVALAEIAQSNFANQELISKVTKLLNQLRTSLLESRLQLVVSSQGLEAMNKELLATYDEKLNVLSTVILPDFKENIEIKDAEIKAKSNLLNDAKSNLQDAQDNLKATQDAWIVRANLNSKLVQQYENDLSLIQQVKQSFDRAGIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBB9</accession>
    <name>A0A8S1PBB9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BPL/LPL catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1120140</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the biotin--protein ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PBB9"/>
    <dbReference type="OMA" id="DRKWECQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="3">
        <location>
            <begin position="11"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8100487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="309" mass="36251" checksum="1E08FC1D5A7502B2" modified="2022-10-12" version="1">MDPQQHKIVDILFEEIDSTQTHATKEYQNLYKGQITVLRALHQTAGRGQFDRKWECQSKRNILTTIIFPYFTNLQYLKNITPVIGYTIVKLYKELYNLDAELKWVNDIELNSKKSGGILTESEQIGDELVLYVGIGLNVNWCIQGATCLEENTGKEVDQEELFQKLRERVIKTLYQLNEHGFEMFREGINQILYRKGQLCDFVDSKTLEIVYSGIVEELNKNGDLIIRGQDGLSRVVDPNVRMKYDIHISYQRKIIIFQNLYQNENFKKLFKLLLISQYIQMVYKLLKISINKLWEMSFSNRFSSIDTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PER5</accession>
    <name>A0A8S1PER5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0910071</name>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1160056</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PER5"/>
    <dbReference type="OMA" id="GENMDQS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000003">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="7">
        <location>
            <begin position="117"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8092674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8101636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="312" mass="35787" checksum="1B4BE961D17D9BB8" modified="2022-10-12" version="1">MASKLNTIEATNLDHQIQHLYQCKPLPEQDVKLICEKAKEILIEESNVQPVRAPVIICGDIHGQFHDLMELFKIGGKAPDTNYLFMGDYVDRGHHSVECVTLLVLLKIRHRDRITILRGNHESRQITQVYGFYDECSRKYGNSNVWKCFTELFDYLPLTAVVESQFFCLHGGISPSIDTLDHIRQLDRVQEVPHEGPMCDLLWSDPDDRSGWGISPRGAGYTFGQDISEQFNHNNKLKMIARAHQLVMDGYSLAHERNVVTIFSAPNYCYRCGNQAGIMEVDENLRQTFIQFDPAPRSENESVPKRVPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PF23</accession>
    <name>A0A8S1PF23_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1150043</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PF23"/>
    <dbReference type="OMA" id="THRKWEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007288">
        <property type="term" value="P:sperm axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043195">
        <property type="entry name" value="TTC12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46540">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46540:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="209"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="288"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="54"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="344"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="120"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8101303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="850" mass="100750" checksum="D46BF1394E8E6FFE" modified="2022-10-12" version="1">MQPPIFESQLDPEEMETWLAEVELVHDKIQKLSTDQVNFKEDDEEIMKIRQKRIESEKKKQDNQKQLQIQKEEEELKGRKGKGQNKDYANFCKYCYLEYIKQTDKCYWCKRDTLTQEQRQNQLLEKVEKYKEDKNRKQQKKQKFELMEKTEKILWKKSTFTHRKWEYYTSSEEEFESEPILPKDDPNFKALELDMQERKKRKEEDMKKAEELKQKGNDYYSKGDFDQAAWKYSQALELVKDNKTLWLNRAIAYIKSNKNKKAINDCTKVINYAECFENGYTQSKDSCCKAFARRALAYYNRDRLEEALNDINQAQELIPNDKWVQDLKKEIEAKINHYQRLKQNEKNEKDVEELTQDKQNEQEDNNNADNIKDKQRVMEENLTYKQIIDKFVGEEEIELLSLFKVLKVDSSESTAYFYEKNGLKQLLSIISKNQEKLNDQLNILASLPALIIQVYQDKSLLYQEQFLIQYNGIEVIYQKIKFLLIQVDTKSQSAIYDIIADYLDVLNVMGEDKPRSFIQKHEIIRTKLYPELFHRITSLYKTEKDLVAIFLSFCSNLCYGQGTPLKNVFFENKNEIIVKFIELFEKVEIKQANIRMMNQLCNLLSNLLTEDKYRIYFLSKELNQNFFKSYFKFIKSIQFKDNEYNLLKENGIAILVNLTFQINQIQTEYVSKINDLIPTLIGYLESEKDSLVIERVLTILTKVNYEENIFIISKFIQQYSNQSILCLLQWLSRKSSIVVNQLKQQQINLFELLEQLKLQLQSDNEMIYCNCCNLIGQIIDLLSPIKGEVNACSIFNQTIPRLLSFVKDKTGNSRKNSAILLAKLTKDQCNLQKLRDLHGIEILQSVIGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PI00</accession>
    <name>A0A8S1PI00_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'(2'),5'-bisphosphate nucleotidase</fullName>
            <ecNumber evidence="3">3.1.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1190100</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.7" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PI00"/>
    <dbReference type="OMA" id="DELHKQP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008441">
        <property type="term" value="F:3'(2'),5'-bisphosphate nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000012">
        <property type="entry name" value="Blast:Putative inositol monophosphatase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050725">
        <property type="entry name" value="CysQ/Inositol_MonoPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028:SF5">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8102827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="36010" checksum="D55987F3B1E65C97" modified="2022-10-12" version="1">MLKVHEFFSVATQLAYNAAIIINQIRLSADIGQQWKGIDDPVTIADVKAQTYIIQQLHKHWPQLKIVGEETTVYDQPIDLPDTNCNLYTEEIFTTTPNNLKVRTQYEIEDLCVWVDPLDGTLDFVQGDFDCVTTLIGLSYKKKALIGIISQPFVKIADKQYEFKPKIYFGHHPQRRVFYTYDTQSQIPFELLKPSYDPKNIVLCTQNKRIDETKLEKIKSLGCSLLQMGGSGKKSLTILESKADLYLYLGVRMSKWDICAPDALFQAFGGRFVGLEGQIYEYNSEDKIYDNPYGHLASIHNELVDKFLPKTTGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJ26</accession>
    <name>A0A8S1PJ26_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1180120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJ26"/>
    <dbReference type="OMA" id="AQNCILI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="102" mass="11957" checksum="300AC7CD1BFDEC35" modified="2022-10-12" version="1">MKIINQNESIDSDSNATIPNNNDQNQPQINTNPTTQTQLITQNQLFKYNSQESTEVLHLNRKINLNYRFLKTKKTKITKKSQSRNELLKHIVEQDLNDLFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PK10</accession>
    <name>A0A8S1PK10_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cation-transporting P-type ATPase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1200135</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PK10"/>
    <dbReference type="OMA" id="MHDNENI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF21">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="392"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="811"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="833"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="874"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1011"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1051"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1075"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="4">
        <location>
            <begin position="117"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8103279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1111" mass="128605" checksum="AA6D94B75FBE875B" modified="2022-10-12" version="1">MNKGEQSQYLLEQSNNTKSFAENSSPNKSLVQYSIIQAMLEFKIKEHPIQPGELINSSIVEEAPRFLERGADYSSKMHDNENIMFNKQKPSQRMSHVYPSYMSENSIQIDQDKIEMIEHTYSVEQLQKILKTNIQDGLNDQQVQEKIAKFGLNKITSKHANVQLKEVFNLFTLALVIAIVLSLIGYLYDEKEHISFLIEIMSLIAVLVFTHIITFTSYQKKKKLFQQFNQIITNDEVIVIRNGQKQKISSEQLVKGDLIELNSNYKVPADCRIILSQQLSVDNFIFTGDCQPILLESEESNHKSIFEARNVAFLGVGCVFGEGRAIVINTGNQTILGKLAKNIDVPMSNSYIDFQVKKIIQITTLIIFSFIIITIIGWILEEYKNGQYDFTFLINTIIIIILSNLPFTLMLLISYSKRKIVSIMQDQSILLKDVDVIESLTQMSLLLINKEAVLVQGQQPKKIILDNQVYHINKIKNYQSLLNCIHYCIYQENYSLEESYNKQDIQQIMRQFIRDYRIDDYKIIQQRQFTAIERYCLTIVQNMQFKSQYIVYLMGSPEVIAQKLFRSGSQEDIEYLNSHKNLAKKGYSLISFAEKVLILDENYRFDIANTNEFNFNLNHATNLGTIALKLTITPIIRTVLQKLNLSGIRVVLMTGDFPETAEYIAQESQLVCNPKIVTGNDLEDHEQFDEDQQQNKQADEESINTKLCTQLNTIYARINPAQKLMVISAFQKQGYKVGMIGFDTIDSPALRKCDVSFALNRSDELAKQSSKVILISNQQQELEQILKCIYHGRRFTDNLIKQIKLCLSNKFGQFIPILLMLFLGIPLPLTSFGILYITFVLEILVLGVLNENEEVDIIIREPSNVKIFMKLQHFLRWILTHGVLAASSGMMSYLVTMHYFGFQSKSLPGIIYKTGYSNQIYNSTDFYNFNSSDQYLGNLNLINPQPLSQNPFITFQFYKHFNKEAQFDFRNVIVKYNETDLSWFTNFITSNSDICKLNDSELCYSYLSLDIAQLGFIYGFIMWGWLHVFLGRTRILSFRYQKLNKKLTYSYLISLFLVLLMYIPYMIYYFPDKDINFIGVIGLPCTPILILYFCFNEITSQRKLRKLKKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PL42</accession>
    <name>A0A8S1PL42_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1210091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PL42"/>
    <dbReference type="OMA" id="NDKKDHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="486"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="215"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="496"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8103524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="507" mass="60474" checksum="A5E34BF308CF25F2" modified="2022-10-12" version="1">MKQANNTNQLQQLDPIEVIDNLETLKKVIKQKDAQIKNMETEFQERLNAFQLAVARDQDGLKQDIISFLLKDNEQQKKIEQLHINYELLQNQLEETFLESNNKIKTLLDINDKKDHEIEQLKQQIRDTEERVNQLRKNSISKDRNMYETEALINKKILDLQKREADLTFKLKQALEKNEQLQNYFENYQSEKEVKLIKQVEHLKKQNTSQLNNISSNYEDQVKTSKEVIEQLQEHILNQKDRFSQIQDENQKLKCEVEIANAKINQLLSIIDKLQDTEQLHDQTRKELQQVEDSKNILERNYKLEIQALQIQLEEATKYIDLTTNLQQKLNQKDSKISYILQENQQLQELVQDLRVQVSSIEQHFQQHADEQRKANILLSNKEFELQTIIESQARNFVEQEQKLKESYEELWLDFQNLQYNYELLKQKSGEDSFRLKLYKTNDKKGNRAFHGSQTRLTVADKDHQNNQETIKINDDAFLKTLESYQKQDQPKQTNKRLDRNVYVRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQ37</accession>
    <name>A0A8S1PQ37_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1270135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQ37"/>
    <dbReference type="OMA" id="WSFENAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="346"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="515" mass="60927" checksum="5755100670329150" modified="2022-10-12" version="1">MIENQKNLHCLMRHELHVSMVNLDSKLTQNQRLLCQECIDNFESEQKYKTIGFFLIIQKIEDNKQQQLNIIENLIITNIKQVESFQIQINNLKSMVLQQLDQLINNTKDWILNQKQIGFKYSEYSFFKELDLIIINQQNNQDDQINCQNQIKILNQSQITKINSKLEQFKLFQEYQQLKNILNKLNEQAFNISIIQQQQQLSPTQLLFIDDTIQQKEIFYAIVFDSTMKIMVSTCGNKIIIWNFKNGKIQQVQTLSGHQDNVYYLVYSKIKNCFISDLGDGSIILSKQLNNNQWQSSKPQFEHKKYIYCIILTQNEDQQISGRGDSMINVLRIDFINNQLTFLYSLQKHTSSVRLLSLNQQERVLVSCGNDKLTIIWQKDANNKWVFQYWDPCQIHKGRLIHMGALFSQFFICIQQKDSQFQEILEKEVQLQKNRDGLVNLSFLIIYITEQNLICLRHINNIILLKAQNDGQLSIFQELDCQHQSIFGTVTNNGQYLIYWGENKMNYEIYEIQYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQ62</accession>
    <name>A0A8S1PQ62_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T1260097</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQ62"/>
    <dbReference type="OMA" id="TYEDRHT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17940">
        <property type="entry name" value="DEADc_DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="68"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="247"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="7">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8105176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="46417" checksum="0665E7FE89A3E63B" modified="2022-10-12" version="1">MQQSDSQQPQTTQTIDEQPKPKDTRYKTSDVTKTKGVEFEEFDLKQELMQGLAAANYEKPSPIQEESIPFALAGSNIIARAKNGTGKTGAYIIPILEMMEIEGQIQSLILVPTRELALQVSSLVKEIGKYMKVECMVSTGGTDFKEDIYRLKQVVHVLVGTPGRILDLAQRKLADLSKLKHFVLDEADKLLSVDFQPLIVKILQFAPPEVQIMMFSATFPVDVKGFINEHVPQIQEINLMEELTLKGVTQYYLFIDEKQKVNCLNFIFSKLEINQAIIFCNSARRVELLTQKITEFGYSCFYIHAKMNQKDRNKVFHSFRKAVGRCLVSTDLFTRGIDIQSVNVVINFDFPRTAETYLHRIGRSGRFGHLGLAVNFITETDKDTLVQIEQELDTDIKPFPKEVDKSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PS05</accession>
    <name>A0A8S1PS05_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1280102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PS05"/>
    <dbReference type="OMA" id="KDICYIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="554"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8105774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="993" mass="118229" checksum="64E3CA16516A50B8" modified="2022-10-12" version="1">MLKNRPILCTEEPLDEDQIFIQSYQVEQEVPNVSVSISEVSQSQEDSKKVFEIPQVLIEHHEQEKLKEEEFRRKLEPKKGFAKLVQLLHIRQFIHQILAKRRLIFKITKYHLQVINDKASSLDDVGFRKYGITIKPLIQSLQENTQAHLGNQQRKQLLMQRTKIFIGSQMQRFYGLIQKIPLIKPDSKMKVVWDSILFICRFYLLFTIPIDLAWPSIQLLFYTLEVPTVICITLLIMDFFVCLFQSYYNQGQLVTDRMSILKHQIGKAYGFEMIANILLIIFYNISNQYDYGFNVFENPYYIFLFLQFVQYKNISQLRQQLEDALNLSREAASLVELSKLFFLLFFVIHVFACLWFWVGLYSETYLGISWLEAKSVLDKSFADQYLYSFYYSTVTMFTVGYGDITPQTNPEMIVSVIFMSVCSVQLSYSVSTVSSIIEQISGFKEEKKRKFNIINNYMQSKNISYELQFQIREYLNYFWTMKRNEESNEEKEIIEELSSRLKQRLIFEANSRILYNSPFFKENFSLNFKKDLVSKITSITIAPENVLDYTKQSQDEHSLSIFFIEQGQIEVFIENEQLSELSRVYKLKEGESFGFLSFITGNPSVEKYKTIGFTKLLIITRKDFLDVIKDYPDDYEIFYSFFEELTFNSESELLTMECFSCKSKTHKAVSCPLLHFKPDREKVIKSAQFSWEQQRDNKIKRKDICYIKAILGQSILNDVAKQVQSDNQQLTYLYEEFDEEKSVTPKVQFIENSDEKSNLKSNNNNNNDQNIQVFDFKEESFKPPNKQKKQLLRKKQTLQDIGDIMQYNKKQKEAKEKFRSIVKKVNLMNDFVEKQVEDSQQKLKSQYREYLKIIQYRKSRIQHIRNIYSNKTIHELEFFELKLKMLLSKEFDINLDVQKSYKYFKPKENLYQVLSRTKISMLSNTNEMQIFLQQKSQQLIKYLFYPYLYIQKYVFKYRQSDNFGETKKKQLFKRNNRISLAFIMQQHSSIRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSN2</accession>
    <name>A0A8S1PSN2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal protein L23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1290049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSN2"/>
    <dbReference type="OMA" id="FLNHEMV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001858">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL23M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059">
        <property type="entry name" value="RIBOSOMAL PROTEIN L23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="15229" checksum="50F121A220DE62B1" modified="2022-10-12" version="1">MQKILNFREYFLNPKGIKIVPTSNFKRATPIERFFDQSVVLHRAEKQLEKNVVAFKLQPYKYSLNKPEIKQYFTKLYGLNVDKVNTINYMGAIKRSPKGGRYREKDFKKVYLQLNEEIEPFFQKIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUC8</accession>
    <name>A0A8S1PUC8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1310123</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PUC8"/>
    <dbReference type="OMA" id="QLEFHTM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="115"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="117"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8106706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="213" mass="25420" checksum="6315A2AFD6298A07" modified="2022-10-12" version="1">MSQSLTIHQELRKQFVLQQVTNLESQLTKWVQHNCPNDDILQSHSDEAHSQSQTHTNVLHFQGLLIYLKSLSENPSPLEFHTMKSLQQYFDYMSQILQNELKLSYQRQTMKINDKNIEKKSQKFSKKSNLILKNWLNKHYSYPYPSKEQVEQLAEKCQLTQKQIQIWFINARGRIGNKMYEERKFKNIVKSKYLSLNSNKINKEKLELNQNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PX16</accession>
    <name>A0A8S1PX16_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1340072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PX16"/>
    <dbReference type="OMA" id="NCNIALR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="42"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="200" mass="24338" checksum="FB37E7F70C0C5922" modified="2022-10-12" version="1">MKQVYLSSDQIIQLFESGQLKYTEENEETESENVVLISQMEYQNLQKQLERKIDLKEDYNEQIRNLNIEIQKIQDELDLMKHQRELINSRDSLLHNLRQQFQEANQREHRTILELSKIKEQQLQLKQQFDFIQEKYNNLKKLNKFELDNKDKQIEQLKQSIYQLNMENDKIKQNRIIKKQITQISLKSNCNIALRKRTNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZE7</accession>
    <name>A0A8S1PZE7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1370077</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZE7"/>
    <dbReference type="OMA" id="CINQFNS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="223"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="567"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8108472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="692" mass="81501" checksum="1C902B7B8B993347" modified="2022-10-12" version="1">MFGQTDFWIVIYIYNIKKDFLILNQFAVIAMLIFCINQFNSVHNKSYQQTIIELGVLYLFDVYLQYKNYKRQEKPNTNEFCRKFNINHQEILNHNNNQNKNKFNHLNQRKNVLSINQINESHYQIYQAQDSIPSLKDIFYPEWKKTYYCYTTEELLKVYEKENSNLFYILYIPSEINNKDIYRVQITRNKAQDYILNFTKLDSLLYYKLKGSKRKFRQNLSQIFSHKLKTPLNQALAKITSMLTDLNVLPYQKIILSQIYIQQKCQLYSILDFLDYVNSDSMTFQTSRITLKQIIQNVKDLIREQCKAKSIKLRILIENIQFKDYKGYIVLNTDSLKLERILYNLLNNAYRHSNKNGTIHLTVEMNQGTLNFAIKDSGIGMTQEMMGDYNMQFQFHQNLLKSIKRSWNHKLGTTLFVANKLINLLNGNKGHLQILKQEEFEFKFSILQELNINKGVSDIGFGSLSSDRSEINLILNQQDHMDEIQEVSDQIGDEPQSQEIKIEQIKHIRNFKQIIAQPQSQVQSKHSQLFNKRSSSIFDVNSFLRQFSFKDGGIDSNQELVVEKDQYIMIVDDEPFNHDALLMMLKRFGYTNFMHAYDGSECINKTKENHSKIKFILMDLDMPILNGFKATSILIELMKTNQIKRIPIIACTAHDDYETETQCKALGMLDIIIKPVFLKQLQVVIKNIPKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1V5</accession>
    <name>A0A8S1Q1V5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1400127</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1V5"/>
    <dbReference type="OMA" id="YAQTEQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035906906" description="GOLD domain-containing protein" evidence="8">
        <location>
            <begin position="17"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="9">
        <location>
            <begin position="25"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8109406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="196" mass="22901" checksum="D0DD513F3D5E8CA2" modified="2022-10-12" version="1">MKYLLFISTLIVLGSSLSTQLSQKPTCFLVNSPEENSNLTVNYIVSGLDEIQTDVFIRKKDGEILIKVQNTKDGKIREILKDKDNYYVCFQSRDKSYKMVSFDFDIEGVDKEYAQTEQFEYMSKELQKTQKNFQNIYRNQHWMSDRENAHAIILDQTEKSVQWCAVIKVGVLIVISLSQIYIVYNYFKDKDINGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5K9</accession>
    <name>A0A8S1Q5K9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1450115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5K9"/>
    <dbReference type="OMA" id="PKIYTQK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="418" mass="49947" checksum="15800AD580E3B61E" modified="2022-10-12" version="1">MYIDPADTKAWADELKEYQGNRKQKVDFQKPKIYTQKEMKLKQTEFNPVLQNYQNPERDLKEKEKDFQKTSTLAQKKMEMVKKYMHEYDLVNLEQIQGIERLDEREKVKQCLLPPNFNDYNIITNEKKSEEYYKNMKVVVQKKVHEKPVINRNKRDFNIITNKFLDNHELKAQEQETAIMSDINDKCKRVRNYDIVAGTYYSDEKEQEFQQKLKQDQLNHGKHFNDRFPPSWKFRESVYLDQTKEIPEEIKMIDEINRNHKKRYEIRHVLENEYRERDLQDQMRTQDRLIKRHKYDDLIKKYDKEFDIITLEKPEIDHIVKALPPKPPLNSWDKVQMTKSGSDAPIHEVSIPEGIDFKDNIQNRNDSQGKVRFPQVYRQGSEKSQKSVSKSNKSGPKSIAQSQKSNLQGTEIKSGGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QA41</accession>
    <name>A0A8S1QA41_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Isopenicillin N synthase-like Fe(2+) 2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1520047</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QA41"/>
    <dbReference type="OMA" id="RIDWKAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF874">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopenicillin N synthase-like Fe(2+) 2OG dioxygenase" evidence="4">
        <location>
            <begin position="200"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8112599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="328" mass="39191" checksum="70E9E7930602C383" modified="2022-10-12" version="1">MRNISKYRFSSIIDLSNRVSNDQIHSQLKNYGYLLVKDKRIDWKANEEYFDLMEQYFEKRALQLEQSGKVDDCFPQYKYQIGLGYENIGKPNYDKESLKLMIDQPILIDRDPKWKYFWPYRYDGQYNPPQWVPKDFPNFERIMNKWQIQLLRTTELVLEILSLAFNEKQDFFYNNMNKATHLLSPIGCDLQKHQINTRFSGYHYDLNFITIHTKSRFPGLFVWLPNGKRVELRIPEQTVLLQPGKLFELMTGGVYKAGYHEAFLTEEAKRESIKQNKRWRVTSTLFAMLNDDTILQTMPYFKDLSNCNQAPISVKEQFQKELNKLSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDL8</accession>
    <name>A0A8S1QDL8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1530117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8113007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDL8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="92" mass="10554" checksum="9087EE1672C4745D" modified="2022-10-12" version="1">MKSKSPLILQIQFLKQFLISQAVDLLLFQTSSQVLIKIKQLAYLVNGGYNSTQTNNSLILQAIQEIWKEKKPVQKIIIELELMSPIQLNILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QHS7</accession>
    <name>A0A8S1QHS7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1650040</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QHS7"/>
    <dbReference type="OMA" id="KVYYMFG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036515374" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="23"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="491"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="529"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="560"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8115568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="599" mass="68987" checksum="7943297FEDBD3905" modified="2022-10-12" version="1">MKILMLSMICVIYTFDLPGIQQKTYSEGQQIPVLINEMTSESTQLPYDYYDLDICKPDSTENQNQNIGSMILGTLTQQSKYQIFMNYDITDEILCVKNFTQTEQNNLKWFIDHDYRVNMLIDELPILSNNYLNNTIIGVPLGIRNINQYSFYNHYNFKIEIYNTSKSDINQTFSINSITVDFQSKCMDSIEIDSGINCPINQIMNITYSVKYLMTNSSNRWSAYLNIVSDSDQQWISISITFTVSLLLTGLIAWFIRFTIKRDVLKFEMLPQEESDGLIDQMGWKQISRDVFRPPSGILFLSVLIGTGIQFTIMTISIFFLSSIGFLYSAHTGHLATVVIVVYVFTGSLNGYYSSKFYKYFKGEYWLLCTLGSNLAFPIMALFIFGIENIALMFEESSSGLDFKSGITFIALQLGIQTPLNLIGSFIGFKIESPKNPCKYGQIAQEITQQPFYLDYFYSCLIGGLICFISVGLEISQIMQLIWKNSYYEFFVSLFFTAILLIIISAEVSILTVYLLLQNQNHRWWWKAFFVPFTSGVYLFIYSIQYYLDSLQFTRFSTILYYFGTMYMASLCLGLICGTVGFLASHIFVKKIYSMIKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QI34</accession>
    <name>A0A8S1QI34_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit TIM16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1630086</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000170">
        <property type="protein sequence ID" value="CAD8115302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QI34"/>
    <dbReference type="OMA" id="ETCAHRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005341">
        <property type="entry name" value="Tim16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388">
        <property type="entry name" value="MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03656">
        <property type="entry name" value="Pam16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8115302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17382" checksum="FE79B070CA893534" modified="2022-10-12" version="1">MVKRLIIKLIIEVGSTVAKSFMKAYTQTAKQQGSKSANPFSEFLNQTMQAANLTHKPMTRDEAFKILQLTPEKTNAEEIIKIYWRQFHKNDPIKGGSFYIQSMLHNAKCELMKDFSDINEKDILEKIKKEEQNQKVKEEDVKQEEQKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKR5</accession>
    <name>A0A8S1QKR5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1630060</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000170">
        <property type="protein sequence ID" value="CAD8115250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKR5"/>
    <dbReference type="OMA" id="KQKCQKL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF307">
        <property type="entry name" value="PROTEIN SSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="152"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="148"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8115250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="246" mass="28273" checksum="3B294C8B6970AB53" modified="2022-10-12" version="1">MSSQDLEFVQENLQILIKTVQELQNQFQGKENQEQIQINKQKCQKLIDALTIKFKQGKFNKSENMTIQMLKKEYAKQQNLFIEITKSIRNPKQINQNEARELEMQNAKKINGKSTYVYDENTNYDEEDSGEKGPNLEQLSEMEVHPDIQHKAMLIQEQQEEIDQIQKDALEVLKIQTEVAKVVVDQGKMLDVAENNINKSNTNVKEAVVELEGAKVEHKQYMKKLGGAICIILIVVAVIVVCVKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QMP8</accession>
    <name>A0A8S1QMP8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1860002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000195">
        <property type="protein sequence ID" value="CAD8117003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QMP8"/>
    <dbReference type="OMA" id="GTIDNCL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="22089" checksum="6B53117753761914" modified="2022-10-12" version="1">MKQNQQWLCQQTITDHTSSVYGLSINQQQNKVISCGCDGQILIIEQSQQDSKWIVIQKIKVETYGQRICFINDNVFIFQPYCKEQMHVYEMNNINKQYLKTKDIIVKGGTIDNCLFPQQYIKSKCLVVNRNGKYVNLIRTKENGEFKTEQSIEFGNYNLFGCMSDDGQYLITWDYSSNEIQIRKYNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNH8</accession>
    <name>A0A8S1QNH8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1700032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000179">
        <property type="protein sequence ID" value="CAD8116235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNH8"/>
    <dbReference type="OMA" id="FYWLEAS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="350" mass="41589" checksum="9C40BD6C30B5625E" modified="2022-10-12" version="1">MDKKYTIEQYQLNEEFKQKVQVGDFILTQTDSFVYSLSRSILKIEYDHLLVLIREDQLLHIGWPKIRIINPLQILSQSSKYIIFRPPYQEGELQKFLMYLQTSLQADYDYQKLVQGMIKKLVNKVPTDPIPYSPNLKSSRVCTDLIFYWLEASSPKFRELLPDYLGYLDYPLIGSFTPDDINTLAKMNVFFQIIHEKEIQKPENTFKSIGKNIQSQGKLYLPEIPSIKQLKKLIKYIPYPEKFIGLLQQQFFWYYLIKLFTMSKKQKTEKLLKFLIKRYSSEKLTKKKLNKLVKRLRILIDIISIINSPFSRKTYLKLINNMIELAFENSQSAKEQVLKMIFTQFIKPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPW8</accession>
    <name>A0A8S1QPW8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1870025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000196">
        <property type="protein sequence ID" value="CAD8117084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPW8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024983">
        <property type="entry name" value="CHAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12770">
        <property type="entry name" value="CHAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHAT" evidence="1">
        <location>
            <begin position="225"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="683" mass="81692" checksum="CA38324BB1224EBB" modified="2022-10-12" version="1">MRNPKKVKVILFGLKKFDEYNYFSDTQAFIERVKPPDFPLNQIGQSQGILLSRLENYVQIKTNYLNKGIEGKFMFSFYNHNTKTFYDMNTLLKDGSNFIFLNLEFCDDQKPLLVRLVFIINREILNQLQIDLVKNKEKLSYLNQKILELYKKLQFESQLLNQINEEINIIKDQDQYQKEKYDIAILYSHPVVDQDPEEEDKYFPVNFNEDISLFLNLIKELNIKISYYITQATKQNLYKVLRQNPKIIYISCHGNLDDNMETCLEYEKELKELKELELKDDRYCCIEQVTETDISNILNQQLLKRALVFLSCCHSGNIAEIFHQQGYPTVGIDKRLKLGEKAANSYFQSFYEKLLQTQQDLQLKECHDQAKKQVQQDLGKKDFQYCESHKHTQKCKKLFETEQSNQLIAFQQQRSCCQPQEDILQHKLDDQCQVKKKIQEFLKKQFQDQTNAFLERLDKVAKQNKGLLKICCCELAEIFFFQDDEFKNDFNYSQIEMEHRSSETFKLFGNENELIFSFIKKGDQTPKNKVQWLKEYLVQIEESKQILTQLLDIFQEKEKSKIIYVQISDNKQTVNDQILNFLKQVAIYFRQRYIEQSQLKDIRIEIIDNINQISKNEEQVQYIFIIKGINQKQWEQSKEFFINQKHIVIIPYIELKPNPPNRIYLMYDEFIKIYEENIKKNPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JLI9</accession>
    <name>A0A8S1JLI9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">PPRIM_AZ9-3.1.T0040048</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JLI9"/>
    <dbReference type="OMA" id="FDADDWN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000199">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000695">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="147"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="449"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="487"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="523"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="CAD8042833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="587" mass="67170" checksum="05653638AD2836B3" modified="2022-10-12" version="1">MGSQCCNGQSKSLNYEIVNDSIKNSSLFKQSIHSLPFTNQMPCNNDEILELKKENEMGLPPISENISQVNELEKEKLQQDLSQVTVAQDPKSTNNIVNTSDQQIKEGYNNRSQSTPFKRKTLTSLGTVKLGADVFVSLKQGSIGKFYSTGSTLGAGAYGKVWKVTHKTTGLIRAMKQIKKSSLIQEEQQRLFAEMNILKNLDHPHIVKLYELYQDAQNYYLVTEYLSGGELFERIKAMTIFNEKKAAEYMRQILSAVMYCHEQKIVHRDLKPENILFVNDSSTSTLKIIDFGTSRKYDPSKKMTKKLGTPYYIAPEVLKQDYNEKCDIWSCGVILYILLCGYPPFIGKTEKEIMKKVGEGKFEFDADDWNQISKEAKNLINRMLHVNPNFRISAKQALSDAWIVKHCSQTTTNLNVNLRVLQNLQKFQAKSIFSQAVLSYIACQMTNQLEQDELLKTFQSLDKNNDGILSREELIEGYNTIYQDKEKAEQEVIKILQLIDLNQSGQVDFSEFLMAAMNQEKLVSLQKVKAAFKVFDANDDGKISKQELEQMIGTLDQELWEQILEECNAQEFITEKEFINILLHQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP26</accession>
    <name>A0A8S1JP26_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0060305</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JP26"/>
    <dbReference type="OMA" id="EVIDCYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000184">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="376"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="118"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8044223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="551" mass="65067" checksum="EA5869FD59C06FE5" modified="2022-10-12" version="1">MQNNQQQLKNSNSVKINKPPIDNRSSRNIYELSADKSQINKSFNDKKKSIPKANAILQELFQKRQNSQEQLFHRTSSPKSQYDNTSINKSQRSISPQLLYQMPFEIKKIYQQINHRLDQQKQQQNNKNKNTQKYKTEESKSHKNESTSSTINDSDESIQNLINQEEIVFTIMAVISRKKKVINQCQNYLNRISHFIFEEKDKNTQQILSKQIHLERIGILVILYKALSDTFEEDQQNLKNLLFYIHNSMILHLQLINQSNILSQSQRALIQARLNKVRPQKNYQFLDINLIRKNCNVVYSLLVLFIENSTDDKLNSLEKNLANIDRITLQQGTEFIKQEYQKILAHIQQLRLTMDSQDFQFDYEEVIDCYQIPYLSKTNKYTLIIDLDETLVHYQEGQFLVRPFAQQFLKDMSKFYEIVIFTAAQQDYADFILDLIDEDKVISHRLYRQHTTLVKNTYVKDIQKIGRDIKKTIIIDNLAENFQFQPDNGIQIQSWYGDSDDQALLLLSPLLIQIVQKKIPDVREALRKFRDQMQRNIEAGISEPHLHLRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQ23</accession>
    <name>A0A8S1JQ23_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0050293</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQ23"/>
    <dbReference type="OMA" id="NMEDQWE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="169"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="415"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="222"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="307"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="502"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="553" mass="65590" checksum="B1D6EC5FF0F370A0" modified="2022-10-12" version="1">MKVIISLNSEQHEIQLQDPTIEALILAIQDQFNITFHFELLTTDNQTLIELKEGMQIIVQKVQNDKVNLQKFKSFFSIAGSIIKSKTISHNFSINKLIQIQKQKSFDQNSQQRIYTPDQQSQQINTNNQSINQQNIQKCELSGILQQDSAMILDEKQSDRLLLNELQEETEQKQKIHNSQEQKKRPKNNSIPKQSQTTKEQAFAYAEELANIPRPNKRQLILQRMEEDKAEKERILKQQQEANRIIKQIQEKMKQKEQLLKQKEEALQIPELEKRKAVLAQKRELSQPIKLSELTEHQKKYEEDRKIKEYEREHHKQEIEFEIKQKSLKFPKSQAQMRIEEEQKLQKLLREQEMEQKKLARLKQLKYADVAQEIYFKEHPLKPKQSPPPKEDQSKQIKEIQITEQNLKKIKRFIPQQRHFSHDSKDSITDLSNHQENRPPKPLIKKQQKIVEKVEKPSELPPKPKTTDYLQQLRQQRKAQVNMEDQWEKIIENKGLENEQKVQNVLNNVQKLEQKAKEREKLALINRDLQAEEEANNLYIASIKAKLAILEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSZ7</accession>
    <name>A0A8S1JSZ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0090328</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSZ7"/>
    <dbReference type="OMA" id="KNFMNAM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="235"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8045301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="302" mass="36675" checksum="B7590F6E72A74785" modified="2022-10-12" version="1">MSINYACNIFYSYLKELRKTGRRQSVNTDFMQYHKLSQDKTFTESFPVSLQRTKPNFKNQLSQLEFDDHQIDFKNHPFTFQDKTDQKTEVTKKKFVFDEHQSTKQGEFKNFMNAMDDYINFLNESSDCLTLNSENHSQLENVLFAKFNIIRQKHEELNKKYKLAQQNYKAQRYQNQLLKKQIVEIHSQLEEEQQKSCILQEINEKLKKKYRDLQNRTQFIQNSFMQDRMKFDHKRQNQYQQSISVDESDRGTTQQTYETNDDQQNVTKFLKSQMRKLRQNNQKIDDYSRLYKKISEHNNDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JUM9</accession>
    <name>A0A8S1JUM9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0090326</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JUM9"/>
    <dbReference type="OMA" id="ENEYCKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="218"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="453"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8045299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="518" mass="63761" checksum="204C8BF2854201CB" modified="2022-10-12" version="1">MEQLLERYKDLEKRNTEELEHDKSITHLLYIEGFKWDELDKIKKINKISQTKVEKRITLVQDFGLLRKDVEPHSTENEYYKILLKDIDMHINSIMFEIDKLEQLKQKSQRFLRLQSQPNIKPETAPTDLMTQTGGLLSSTSDGQTRMYKSSFQFYRAQRDQQIQSHNQKWIEKQYSIQRKLIEDEKEFQKFRFEIEAKQMKAEMNLEEYQQWQARQLKEQEELNSKRLQLHKDRMQKDQQQRQAILEEKMQRKDKQLKKQMSLLQDFHEEQKQIKEEHFLRILQKSMGALNDKDKFNQYQKEQSEKKEQQNLKKLKYIEESKQWTKQQLQTQSALKQQKIEKAYQILAAQDYERREKFEEKEKKKLSYMDKYKKTFSEYQIKINKEKEDKMHNFQINKAIEDASKEQLIKYQFERRSNSQAQGQRLKERRLKIFQEEKAGEHLIKQLEINQRLNQLEWEQEQKRRKQQILEEEQRQRQLMIENDARETMRQREKNTRKLEIEKELREKEMKTLKYQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JUS3</accession>
    <name>A0A8S1JUS3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JUS3"/>
    <dbReference type="OMA" id="HDDNICQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="211" mass="25214" checksum="E8B6025CEF2E1A3A" modified="2022-10-12" version="1">MSRFYAKKEFNSEYYTLLNGLEQSFFKEDIDIKDLMQLILSIAELVEYFDLKKDPIKNYFLEKMQYILSRPFTLCNLKKKEDENTNNKEMKKSRHSHFSSNVQKLEMQSLLQSNSEENEGTIKLLQEDYEKQLNDQTQLVQMDLNNQIGIFKNKLRHRKNKSLFSPSSRINNQEDLQRRSIRQNTCLSSNSNRRQIIHDDNICQILTQQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JV13</accession>
    <name>A0A8S1JV13_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dynein axonemal light chain 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0060393</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the dynein light chain LC1-type family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JV13"/>
    <dbReference type="OMA" id="NCERISM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000049">
        <property type="entry name" value="Dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF73">
        <property type="entry name" value="DYNEIN AXONEMAL LIGHT CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8044310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="201" mass="22660" checksum="35E59A39D8C964FE" modified="2022-10-12" version="1">MAKTTCAKALQNWEQEHPGEQPSEAEDIRLIFQNPPLDKLDPPVLNTLAKVKRLSLSSNAIEKMVNLPGLRQIEILSLSRNNIKKIAGLEEIGQTLKELWLSYNYIERLDGLQPCVKLHTLYIGNNRIKVWDEVDKLKDLPEIANVLFVGNPIYENQKDDPKLLVLKRINTLKNVDGTVVDDSLMEKVKALGDGVPTTTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYN2</accession>
    <name>A0A8S1JYN2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase III subunit C6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0100043</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JYN2"/>
    <dbReference type="OMA" id="FSCDIFN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007832">
        <property type="entry name" value="RNA_pol_Rpc34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016049">
        <property type="entry name" value="RNA_pol_Rpc34-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12780">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED , 39KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05158">
        <property type="entry name" value="RNA_pol_Rpc34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8045820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="23676" checksum="6C35D26802075F79" modified="2022-10-12" version="1">MEIQLNEEQQKIYNLVLNSKKSGITKANLAKNAGLNTKEVEKIIKTLENRDKIKSVRPNGRTTGVKLWIDFNQTLDSSLPTNVFRTAEQGVDDDLIERIKQKLIYFMQQQFDGRASLDEIRRVYKAQQSEHYDEMDFDRIINLLQYDSILIEEENSFFKLNPFRQQKFQFYLSYLPCQTCPVFNQCKPGNTISPEKCIFEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K218</accession>
    <name>A0A8S1K218_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Dynein heavy chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPRIM_AZ9-3.1.T0110163</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NEKESLX"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000005">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000049">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000362">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF68">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="15">
        <location>
            <begin position="21"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="16">
        <location>
            <begin position="210"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="14">
        <location>
            <begin position="672"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="17">
        <location>
            <begin position="822"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="18">
        <location>
            <begin position="967"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="52"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8046910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1285" mass="147616" checksum="A6ECA2FD31C82FCE" modified="2022-10-12" version="1">MKGLEVGKIKQVTQNKIKAKIASNPEFIPNLVQKVSVAAKSICEWVRAVSEFTDVNNDVEKKKSQVEAMNQQLEKANKILLQKQSELAQVVKKVTELEIQFNQNKQEKDRLDQDIQTTEQRLIRAEELTVGLADEQERWKIKVESLTEEIQLLLGNVFLGGSTVAYLGPFTGTFRNQLIQNWMEKATELTIPLSEKYNFESVLGDALEIQQWAANGLPNDTVSKSNGIIQKYSRSYPMFIDPQLQANTWIKNSYREHNLKVLKITQEGLIKHIENAVQTGIPLLLEDVQEQLDNFLEPLLLKQFNIINRRKMIKIGDREVEFDPNFKLFFSTKLANPQFLPEIFIRVTVINFTVTEQGLEEQLLGDVVQIEKPEMEEEKKDLIKRISTGNMNLRKNEEKILNLLANSKGMILDNVDLIENLKISKQDAIQVKESLVNQEQKSAEIETARQQYLPVATRGSLLYFVIADFTLVDPMYQFSLNYFKRLYQNVIRNSEKNDDIKIRISTLIDGITETIYSNVCRGLFNQHKRIFSFLMTVKIQLNAKQISFGEWNLFIRGASLSIQPPAMPNTVKLTPKIWNELYQLTTVHQNFIQIYNQTLTNFKETEQLIQSDNPWSLLNEQLTPFQKLMIVKVLREEESLYAMTYYVDAILGKKYTSNNQASIEEIFNDTDHKTPFIFILSQGADPLSSLIRLANQKKISSEKLRIISLGQGQGIIAEKAIESGVKSGDWVILQNCHLGKSFMPTLEKKLEWFEDPELQSQFNTGFRLMLTSMPCDYFPVSVLQNSIKLTTEPPKGLKSNMFKSYTDLSSEQVENCEKKEPWKKLLYSLAFFHAVVQERRKFGPLGWNIRYEFNDSDLETSYTLLRNFLDQPQEIPWDAIVYVVGEITYGGRVTDDWDRRCLLTILAKFINEDALNDGYSFSDSGVYKQPAEMNIDGYRNLINKFPDFEKPEVFGMNENANITFKLTESKMALSTILSIQPRESAQASDSDQKAKTPDENVLELCDILAQKLPFQIKEQEKKKKQAAPQHTSSEIDSLKVCLNQEVQRFNKLLSVIGNSIKNLQAAIKGEVVMSAELDKMYSSLLNNQVPQIWANKAYPSLKPLASFYDDMIKRVNFFRDWFNLEFGYPKGYWISAFFFPQGFLTSVLQTFARKNQIAIDILGFSFKFFNYVDSEMITSTPDNGAYIYGLYIEGCRFDINKGILEDQLPGQTIYQAPIIHFIPTQDYKPNPNEYSMPLYKTSLRAGVLSTTGHSTNFIRAIECPTKKNPDYWILNGAAFTTQLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7Y3</accession>
    <name>A0A8S1K7Y3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7Y3"/>
    <dbReference type="OMA" id="NTQTGCN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="515" mass="59508" checksum="FFABCB220F1C5242" modified="2022-10-12" version="1">MEFTLTATIFSFFGWNVYLFIKKYRERQRQNYLQNLIDQESDYLIMNVAPYKKIKVNEDKQTIIKPIQMEEAESSSDDFELISYKRRKTILEEVKQEKFQEQTYIENIKSFDMLPVPSRKDRKLNKSNKDFSFTSQNLEVQEVKRFRKNSKIDDSLPIESPKQFKQNSSNKVSEKKITQFEFSNLKDLHEIQDSKQESDNCITSQVTQANQIQNISILPENKNISLQFPVQVQNQNEQIKEQSNQQLGAPENIFGKLIFQLKPQVQQEQQQKQTSPQNQKLQSPQQNQNGLFSNLLKTNNTNDLVSLQQNQMPQVQMINNLQQQQQQQQQQQQQQQQQQQPELLQAQLPQLEKQEQKQISLFQNITPTNNTQIPDFNLDNQNKDSIINGSSATQNLLPANFTFGINSSNQSVSQGGLFDSLFNKSPVQFGNPTTTTLNITQDETGNKQNLLLQPSQQIQNNVIQSQSNMGTSLFGNMFSTQQPKEQIQQTQTGGLFNFSSNTQTGCNLFSSLQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-09-13" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9C8</accession>
    <name>A0A8S1K9C8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035825125" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="34441" checksum="9D869EA06003C3A4" modified="2022-10-12" version="1">MRHFLMVIFFLQQSILAIPNTLSEYQFSITLIVLYLYFLDFSFVVTCPQPGLIWTIRYEWIAINDQRISVINSFNMNPPSDEVFNHQLLNANAAIIGYSEQLDFQLSITQLSVDNVSVGITKITGEFENLTLIGYQILLIAFREIIDLGFFTFIPVYNRNTISLQSVNCLQCQFKELKYNYQSQLTLNLQKTFGISSITQQFQRCKMYVNKGAPIKINHITQSKISGIRMPNLKKLVRNQIFHQVIDLNFMLKFCQFLSFXSYHGFRINLDCKYVLILCLTNNQKHDHLKHVDVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KC78</accession>
    <name>A0A8S1KC78_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KC78"/>
    <dbReference type="OMA" id="NFQTNGH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="212" mass="24136" checksum="C90BB1DE08F28FDB" modified="2022-10-12" version="1">MSQKFEEIARLTRSTAIFKANYVPLTLVGASLISFIAWSKLPYGQAFPHLTISEYVPKKQYFHSLILAPLNFQTNGHLLVYGPAMLYSFYQMSTVLCNAQFLAFYIINSLICSSFTVYYEKKRSAENGINLMSPKCVSATTPLSFMTSLMVLKPHLKLLNKPPLPFFLVPAMYGMYELQEYQNGFINEVCRPTHILAMINGLILGLIFKRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KE71</accession>
    <name>A0A8S1KE71_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0190279</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KE71"/>
    <dbReference type="OMA" id="TKFFIIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000654">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="11">
        <location>
            <begin position="282"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="347"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8052631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="423" mass="49230" checksum="568B70A564DBFEC8" modified="2022-10-12" version="1">MGNCNNTRDLNAGEIKLPNKCDYTFLYVIGRGGFGRVWRAEHKKTKHQFAIKEMQKCKIINKKSIGSVMNERYLLSNLRHPFLVNMHTAFQDRESLYLVMDLMTGGDLRFHICKNRKFNEEQTKFFIICVLLALEYLHHNTVIHRDIKPENLVFDRNGYLRLTDLGIARIWKPGNDGDTSGTPGYMAPEVMCRQVHGVASDYFAVGVIAYECMMGKRPYLGKTRKEIRDQILSKQITIKPNQIPEDWSEEAADFINQMIQRKPVNRLGFYGPDEVFAHPWFKNVDWDAYLTQQKQAPYKINSNSDNFDYKFANIKEPEDEAQAKLNAELLRRDSIQEAFNGYTYKSQNSQQTIQDQTNSKPTTSMSQQITPTKKKLLDSEPQVELKSQKKYHLVQTPRFNKKLNFTADKPLTERGQTKKHFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKN1</accession>
    <name>A0A8S1KKN1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6815 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKN1"/>
    <dbReference type="OMA" id="GIMEACK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049212">
        <property type="entry name" value="DUF6815"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033816">
        <property type="entry name" value="Cj0069_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20668">
        <property type="entry name" value="DUF6815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6815" evidence="1">
        <location>
            <begin position="218"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="343" mass="39192" checksum="B9BEAD47497E7B2E" modified="2022-10-12" version="1">MAQNKIHKRVAIFEAEGGSDKTWNRHRKDTMPIIQAFKELGWTAEVIFFRDEWKEAITKYVIENCDAYIPRINTGNLPNGEAVFNQALREMCAAGVVGTPHPDTLMKYDSKLSLVDLNKTPLSLADTVAYFKWDELVKNFPTSLTNGKRVLKQNRGSTGEGIWRIQVAEGVQVVKGQALALDTKIKCNEAVDNHVEHHTLEIEENFLIDMRFLPRIKEGEVRIFLMGTKPLFVIHKKPADKQDAFSATLFSGATYKYESPEAWPELIKFFSSCLQHLTDNLGDVETILDWTCDFILDTDENGKDKYWISEVNVSCIGFTNQLDIGIQQEMARELIRKVLKKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KPP7</accession>
    <name>A0A8S1KPP7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0260004</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KPP7"/>
    <dbReference type="OMA" id="MQVASKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24141">
        <property type="entry name" value="2-5A-DEPENDENT RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24141:SF1">
        <property type="entry name" value="2-5A-DEPENDENT RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="445"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="478"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8057360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="581" mass="67553" checksum="26DC4AD80F952A03" modified="2022-10-12" version="1">MIKLGHMLAQEQARLRSEMQVASKQKKFRNATASTFYPNISKTSTLLQASPTNSTNVISYNCSPISSQKYYSFSPDPDQMIGLKKNYVQTSYKNKLETIQLAPNKPPLKRGNKVTNLRNINNNNASQPHSIRNEESKEKLLEQTHFLCAIMQQQHSATGKIVANFLNGNLEKNSYESIIQMEKSAQALQQSAKEMSKALYASDDEGEFENLQNKFIDQIRFSKYRKPTQEKNIVFKRNYGQKHEMMIDQVAQQRQKTEENQDFDVFIPKSQMDQFFKIIQDKIDGRMKEQTEQLIKEEQKLVNGMITNVKKFREDIREVKYTMNHVRKNKKALQQQQPQNTLRTLRTMKSQSSVRMKSDQVTKLDEDAKADLKLRVMKALRNFMDKLKRFNITLEDVVNNQVFPSQAYERPNSVEFFRQVKADNIREIENMLRDCRYHVYDRDNQQKTALHHAVSINSIEAIKLLVENGADLDARDMMGRTPLHIAAKNNNCDTVRVLLVYQANPSIKTVAGKTAQDLTEMPVIKALVKNAKKLHFMMNLQPNNKKKDFWVEKAVVYFQEDDPEKILKLQLYKTQAKLFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KW85</accession>
    <name>A0A8S1KW85_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SUI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0290138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KW85"/>
    <dbReference type="OMA" id="MYHAGQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="2">
        <location>
            <begin position="22"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="12441" checksum="469512C23ED7BCB0" modified="2022-10-12" version="1">MTDNQFDNQNEFDEMANFNFVIHIHLKKRSARKTQTIIVGIPDEFDLNKIIRFWKKQFNCTGGIISKDEDYGDQVTIRLTGDNRQQIAKFLVEEGIALQDNIKVHGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXA3</accession>
    <name>A0A8S1KXA3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXA3"/>
    <dbReference type="OMA" id="YIIHFQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="108" mass="12972" checksum="1C5C0BB48F6A51B9" modified="2022-10-12" version="1">MSFQIQNIILILIIKQRVLKQNIFLSLRIIIYHVRLYIIHFQNQIKNESNFPKHPSSKINRLQVKKEQKQEKIESLVFTPQNANFLEIDIIFQQQCVFHSLKIEINSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYG5</accession>
    <name>A0A8S1KYG5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0290264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYG5"/>
    <dbReference type="OMA" id="HAKTFIP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="449"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="484"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="549"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="625" mass="72315" checksum="1247C485BA5B397E" modified="2022-10-12" version="1">MADQEKNLEPPQDQPDQEQFLLQQAQDNRQVFDVENQQEIQKDSNGFKMEDMNNEEESRMMKRDIIKKRKKREKKEKLISARRLKRMLKKKPIDKIRLVYQAYFFIVSITVLTLSILSYRDQTVYEGDYQQFIDNVKSYVIDDIQFTYTNKNCKTQFGSEYSSLYEYEWPGTRIGCDCSKGFNFSTLTDYNIDAFFSESFMLGRICSQELLSKNCNTVNEQQPKIFNSWNDGSYGRPFVLCARRKIGIDLQSNSSNCQSDHKVCGTGDNVFCVPTDVSCPISEIGFTTNSNYQNIKSKNNTNIGDIFDLGAGFYFYYIKNTSSLPIVEFRVTEGDKVCRRNLDQNISPNRIDYPLMLSRRKQCENTDQLFQIVHSIDEEQFYLSNNVIYLSDKLPYFNIDTKYNWTLHAKTFIPWAPQCRGDLFDQVLQEGNTLHYVYISLRVQLGVTIAYFIVIGLIFNIVGTMTACNFAWSCMATRPESQYNYIFLIEIGFKILLQITEIIVIIVSFALIDGKRKIIKQVNDDGCVSDPVSDYFFTNLESDLTKFAWSYNLANLVIFAVTLILDLIIIKHSINQGHHHGAKKHHEKLEHQELDVGSNGESGNQQEVKSDLKLQVKTEALQTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L085</accession>
    <name>A0A8S1L085_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0300281</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L085"/>
    <dbReference type="OMA" id="KNIAYCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051242">
        <property type="entry name" value="WD-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324:SF4">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="83"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="294"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="385"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="625"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8060727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1226" mass="142614" checksum="4F70B20934FBB676" modified="2022-10-12" version="1">MNNLLKRFEPSLTDVIKYLHEHQDMILVERNPYQADRLMMMLDNEKLDALDKEFHEHPNGIELPNFIWLMKCAISHAPEDKYELVNGLIKLFQDIDINGDGHMEWAEFTQYIIDAVIGSKDAQLYDTRFEKERELTEIEVLDRAYSRKSKRYIPMAQVDDSNHKNPIKNIAYCQQLDSVICLEQNSQHLRFYTPECQDKKFTLAPESESGKIFIIHFHTVDNYIAAVTSDRQLIFWDATTFKVIKQFKQEVLQTGIWYFPYHELWVTAGSDYNIRGWSIPWNEKDSKGQVIKFLNAHTKQITDIVELISPKLLASASQDGKIKLWDMQDQKFITELKTPAPSKRGVKGLSYNQDYGSNLISYGFETFINVYCPEVSITRAYIGRLEGHSSLVVTCKFIPQSPNCVSIDDHTNIRIWDIRQMSSVQVIPNDQQSLVTDLCIITRTDRFVYSGKRLNYFYNAAQMSSNQKQKGPNEEVYPIYVEFNMYFNQFIVLTKFDLRIYDAMGGKLKKVMNEVFDDKIQLDLSTFCFGGRQRKLFIADNAGLIRQYNMKTGEFLKKVNMHNEIENSEFANKLANIKKRDTLDISSIIFLQEEKLLISASQDSTIRIYDETDPEESILLKVFCGGHQNSEILSMSYSSNFTMLATGSANGLISLWDFETSKLCGVLNTPQPLAEVTALEFADPYPVLVSLQGILISIWNIKTNKCLLRINTNPLSPMLSVAIFSDISSGPSRQELLPEFAGPLVDKTAQDKKSKLNLQEEQEGLMKDIQQYDTEQHQNKRRALLYMGDQKGYMHILSLTEFLQRKGITEMEKLKKGHSYQLKRKDLIDVSKSVETFLVQEEKQQSPVLTCNISALLIRQWVAHSSAIVKINKIRELVSFISSSMDKHFKIWSMKGELWSDISLAKYDGSNYWKFPFDWVGQKLKDIELVFDALKLIEKENLSPYEKERVKVRFLVNKYFNEAALEEMQRNYAQPEQVVVQREKKRLIQSQSQPMNIALKSIAEPYKELTQKMKDDQLKKKKLELPEVLEEPKRPQSQHGLVQRLMLAFNDNKDIAKEDGIDQKKKQPNKNAHQKRIQELPQMKPSTSHSTFLQKYQRNYRFIQTQVQTRPHTESKKYKFVHDIKYQFGESQRAMKIKYYQNSLKFQSVSPQLLCDDNEILQMKCKFYKKQNEDLIESESSDKSYEEDDLGHPKQHKQIIASHNFLNNAQKNDHKVLNTKKKIFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2A7</accession>
    <name>A0A8S1L2A7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0290047</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2A7"/>
    <dbReference type="OMA" id="QITYFSM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052628">
        <property type="entry name" value="CFAP70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44314">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44314:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="758"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="894"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8059552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="955" mass="112565" checksum="8DFC78EC71997D76" modified="2022-10-12" version="1">MNDLKLLERMPARKTNIKMNFVINGIFSFPEEWRVTDEANPNLFQYSVAFLDQRLSGGKILPRELTEKEKREIEEAEAAKKAKKQPKKDAKIEPPKLTPEEEKEKLLKEQMELEEQQRLKAEWDALTEEQQFYKTQEDQFKSPSIKFDQNQFSQELTGQKLVIQQERVRVDKGEWIFLYKSPIPGDEEVAKLKKAKPKTLNINDLNIIVFKAWIDYIPFQTSGQIETIQRIKFTQYMDEQSPPETPKPNIETMYMYLKINLSQPITPIRYMPQPTFQDLIPLAPEPQPIPLSQQCIEELRSDLQVIIESLAMEYSNMFSKDLNKYQNEKQSNMLMTHKKQSLQQRKEHFLYDFNISGKYKILKERIKKSIVKLCRDKFGKFGSVTGISTDQSDQFYSELYVFLMEEMRQVLINMVRENKEALQEDFSHLLSKKKTIQDVGFQEIIIEKTNETRKQQLERLCQEYEILNRTEEVEKHLRDLNLINAKDQDALMKFAFFCLRCAKIEQAVTLVEQALSFDGHNQQNQLLLAGLYQKREKISEAKFILELLLDQDPNNLIYHLFYNLIIQQSDQELADFMMQKAERIWLRQLNLIKQEGMHHDDPQLFDIPWIKIPIPVPDKPEKDKAILEQREQQRIAWENAHNQPQLTEQQQDQMILQLVEFFENACLFEQAEQSLSLLNDQSHSKIAMLRAKIFLYKSEYSQCLDLIENMLEFNPENLEIRIEYIKILFKSQSFDKCEKQIKFVLPNPQLREDQITYFSMYLMLGSIYINEKQFGNARAVFTKACQIKENSSLSWMGLGISSHECGKYKEAEDALRMANLYEPTNDTVWGYIALNCLKDGKRFRDANSALKEMNKTDTGQVNLMVRLSKEFYNIEEYLTAKELLQKAINWAKIQDMLSEEINQFEIQLAETNEKLGLLQQAIDIYSEILNGECGSLEEHLKEQINRLHFQINIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3P1</accession>
    <name>A0A8S1L3P1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0320327</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8062097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L3P1"/>
    <dbReference type="OMA" id="YRNEIHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="122"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="184"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="372"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8062097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="658" mass="78162" checksum="1121D6DAF97CC85D" modified="2022-10-12" version="1">MQYQTFKPTILNDTTNQQQQGMARPSSKFVSQKIDHTNSINENKKSVAQQIEAIEYIQEIEEQKSVIEGQNQFIQILRNSIESKLQIEGFQMILDQVENMSKEQKIDLFALITQLYQELSIQNQQNAQVENLQQLVENLNKQINCYAKELTELKVDKQYLLDQLDQLQGITKDDDTILFKDAQISKLESEIQHYKTQNQKLLLQNEKYQEDLTQLEKRLNKIHLNLKTEECEKEELIKELENLKQTFYKSQVKEEHKLELNLALKEQELEFSNNKIKKLEAQIKELNLLIEEQQKQIGTQKYQIENLQQQHQLQYDQLEIEMNQYRNEIHEQQMYCEQLQSQLSVSEQFIQQIIDEMRKIIKTNDSDIFNILQQLSENIQTLNSNEQRLKQSEHEKSQIIFDLQHQFTDSQQNNQQLSEQISLLTAQTQQYQNDIKQLEKQKRLFRQEFAHMKQLEEQKNQEIKELEKQFQKFDKKFSEQLERSIKIEKENAQLKEQLSLSQQQLANAKSQVSNKKSLSFSQSSFTEQKAKNYDYIALLQTFEKVKPLFPSEALDLVQEMHTLQNKLVRTDEIDSQLIQEEIKIQQKISIQQLNLKNMQAYLSKILSENKKLKLELQMNQPIHAEQSQSQECLSYDQGRSFSRFHKQSMSVHTKPKCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3S5</accession>
    <name>A0A8S1L3S5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0310036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8060895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L3S5"/>
    <dbReference type="OMA" id="IWEYNFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="246" mass="29336" checksum="74ABB4D814597FB3" modified="2022-10-12" version="1">MQLIKNNVFFQLILSEFILIWEYNFMYKQNDYLQGWDIEQSVDSEENEKKQVTQVSNITKTQPSDRLSFKKLFLKSPQLSPIRVNPPNHQTTPLRESIKQKFVFGNQLMNKKYEMNKGCDVDIEQSHQVFNVKLQQPINIANLRIKLNAIPKQPQHTKAYTQNQLQAMMQLKRRNLYLRSVLKKVCHESDERYSNHQKAQQQEIFLGYRYIDEDLQKLQSPSNSMNLQQQSRKNSNRRPVKVKQQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5F8</accession>
    <name>A0A8S1L5F8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0330273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5F8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="213" mass="24351" checksum="0E7E6807677B87D4" modified="2022-10-12" version="1">MGSSNCCSLSSDQQSEIVLKIEDTEKSSIKSSKKSIKKQQEQCLLQFKSPSQKEKLSVNPNKCEDPATPGFADLRSLGSKRSEYSNKDIQLVAEIVMNLEDLPKQIADVQKSPIISQRSLKKKRKDDSPLCDFQPKNSLRSFDSKKLNKLKSFSEHRVTFEEKSENDSHRSNSADNKSVKSIMKQELKYSQFRKMQTQGDVESKRKVHFQINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L620</accession>
    <name>A0A8S1L620_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L620"/>
    <dbReference type="OMA" id="NIQYSQH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="140" mass="16923" checksum="049B0A9727360BFB" modified="2022-10-12" version="1">MKNLIKANTHKNEIAQNDKVLRDIELYEKIFLSNKYENKRQQSLSIRRLLPKIQYSQHQQSTINSQQKFNTQFDQDTSYSNQYYQDIRKKHIHFSLIDCSKFLKSQNSTHYFQTKKQLSPLKQKIVIVNHQGRPQTYFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9B8</accession>
    <name>A0A8S1L9B8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0350156</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9B8"/>
    <dbReference type="OMA" id="PDIEANC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF530">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE DDB_G0271350-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="173"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="243"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8063819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="317" mass="37478" checksum="7D9D789B56FD898C" modified="2022-10-12" version="1">MHKKIEIILCSISYFYNVQQQNNYLLYDFSLPEDNKQCKAVKVDINKLKNATKIDQALEIIPEQNKLLEKKRRAYNFILPFKQSNCITAHLKQLITQTDGLSLSLDSDNFFDILLEYKDLEKLINNDNIVENLKIFHLLLLKELVREIYIICESQENLVSRFPFYYQTFEFNFPNEIIENQLYLGNSNHANNLKALNLLGITHVVNCAKEIPNYFNDVKQENKNFIKYFQVPVLDLEEEPISDHFDEAYIFINDALSQQENKVLVHCAQGKSRSATIVLMFLMRNKQWTFDQTYEYVQKRRETISINDGFQFQLLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LAL4</accession>
    <name>A0A8S1LAL4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0360357</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LAL4"/>
    <dbReference type="OMA" id="TICTERN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050602">
        <property type="entry name" value="Malonyl-ACP_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13090">
        <property type="entry name" value="ARGININE-HYDROXYLASE NDUFAF5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13090:SF1">
        <property type="entry name" value="ARGININE-HYDROXYLASE NDUFAF5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="3">
        <location>
            <begin position="69"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8064898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="366" mass="42395" checksum="EDD128F7939182D4" modified="2022-10-12" version="1">MLYKISRYFSKQTSQKTKQIFDREKHIINRQYALRTKIENIFLKQSSITLADNLSSIRKQYDGNIAYFGQGAAQFLQNLPNNLSPKTIYICDLELDFLEYSLQQIQNDEQLKGKFKILKVSEMTSQDNKTILVPICCDEDFWPFLDGHLQLIVSNMNLHWVNDLQVVLIKWLESLEPDGTLVGSIFGSDTLQELRISYSLAENERFGGVSQHVSPFISITEMGNLLTRLKFTLPTICTERNLYEFDSIYHLMQYIQDIGEGEALIQKRIGTFKQTIQSVSAIYESLFKNENMKVNSTFEQIYFSAWKYHESQAKPKSRGSATVSLQQLQKEIHQIAPEDKIIFGTLTEDDYQENIIKDEKGTKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LB53</accession>
    <name>A0A8S1LB53_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0360051</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LB53"/>
    <dbReference type="OMA" id="CCIPERI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14499">
        <property type="entry name" value="CDC14_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17657">
        <property type="entry name" value="CDC14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000006">
        <property type="entry name" value="Dual specificity protein phosphatase CDC14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044506">
        <property type="entry name" value="CDC14_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029260">
        <property type="entry name" value="DSPn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14671">
        <property type="entry name" value="DSPn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="177"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="257"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="362"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="365"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8064289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="397" mass="45655" checksum="438F78707309BFCB" modified="2022-10-12" version="1">MQSNQTPIPGSVEIIKDKLYWISDRQAPRNQPNAFYFCIDQDLVYEPFFADFGPLNLGHTYRFVTELEKLLSDKTYQQYAIYHYTSLDSAKRANAAYLMGAFQVIILKYSADEAWKPFLNVKPAFQDFRDASYGQCTYKCTILHCLRGLEYAIKLKWFDVRKFNLRDYEFYERVDNGDLNWIIPNKFIAFSGPSATQKDADGNRTFTPEEYVPIFKQFGVTCVVRLNKKAYEEQRFIKAGIKHEEIYFLDGSVPGDDKILRFLEIAEKENAVAVHCKAGLGRTGTLIAAYAIKHYRFPAPDFIGWIRICRPGSILGPQQIFLLQKQNWLISLGKDSPIWNQVKQIAAEVNIEKRLKDLQLGPMSEADKNKAEKGDQDQAQTLNKAKLMNQNSPGTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LC76</accession>
    <name>A0A8S1LC76_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0340136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LC76"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="106" mass="12761" checksum="C0EC40B692A6F584" modified="2022-10-12" version="1">MVSSVNGFIAKQKHINNDFLWKKIFINKIIYFQMKLLLLALNLSNEHYNYINLKEKYFVTLFNQVTSRLQQILQTKNDQMLIDYSQYLQKMYLIGLKRIHLHSNCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCI3</accession>
    <name>A0A8S1LCI3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0350269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8064045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCI3"/>
    <dbReference type="OMA" id="KYMICGI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8064045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="488" mass="58500" checksum="B1490836D0A6E00F" modified="2022-10-12" version="1">MDQRIYLIQADPINFSIDKIYSLLWSTISKTKQYYSCHNIFICPHTVQIELNPQINEIFYQPRNCELCPKNVFVSLPQRDQLKYMICGILYFLLEGGNNLQQYYQSQEFFFFKNIYGYYNENSYFMRSICQILTRLDFNQKQSILNNMPQSSRPVVEQYLFENYCVIIISPCLGAPQFRKTESSSAYYLISFEKFLLELRDIQINIGMNNPLPPPCPHNVFISKGYQNYCRFFINIYQTNPICRCQNRQMGKILYILLTGKELISYNQQENDEILDNNTIEFLQSLINEYEFNSAQEQFEQYQKIIQKIAEFKSTMSIIVGESIEPSFYANALANNLSQIQINQLWEYYKILFLQEMIELLENRVLSQPIHLDILLPFIFQFKYQQIQRIQAFPQFQESNLLQTILQSYYEQLQNQHNQLPQLYQIINNTNYFPHLFLVRYELFQHFSEVLEFHDQNNQQFRLRFNGDLNRQNIINFENQLKDGQIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LD47</accession>
    <name>A0A8S1LD47_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tubulin epsilon and delta complex protein 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0330240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LD47"/>
    <dbReference type="OMA" id="VQAICNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043535">
        <property type="entry name" value="TEDC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027996">
        <property type="entry name" value="TEDC1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35076">
        <property type="entry name" value="TUBULIN EPSILON AND DELTA COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35076:SF1">
        <property type="entry name" value="TUBULIN EPSILON AND DELTA COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14970">
        <property type="entry name" value="TEDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin epsilon and delta complex protein 1" evidence="1">
        <location>
            <begin position="99"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="433" mass="50570" checksum="0684852E7B187E7A" modified="2022-10-12" version="1">MQTVKETVQAICNILQAFGINNIDPNVFRQAKHNLSEAIKPISSIMHNLLILDLFDFEYKLNEITNTHADPDGLEIILLALRNSPFIIYKNGKPGMDYSSSRDLLLVVGQILAESDIFEKYKKLIEKKVANLKIKLINKTKEQEGEIPALKFISEGSQVKDFENLNRIIRHQFKQLSCLLKRKEAQQNQIDKIVQQMNLKDFTVDQLLSLSDPDQLNEITANLKEIIQFLEFETSMHRHQEIFWAWLESTVDEDKKDVRDHADYGFPDETIIFSPGQQMIKLIEHTQMKLQQLSDQFELFESISSQFEDAWEKVSQLLKEDPGSEQKLQQLQLQIHKIVAQNNIKLMSLKTFKEQVFSEKDLLYCQANDFIKYAIKHNFAQSSNHNESNGFEQKIIYQQEHFASRIEQLHQYIEDEAGRIKVHLKELVFYPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LEI4</accession>
    <name>A0A8S1LEI4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0390114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LEI4"/>
    <dbReference type="OMA" id="LHDANNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="213" mass="24805" checksum="6EA6C865B960CB1E" modified="2022-10-12" version="1">MDNTFHNNICTLMFTNLHDVNNSSDILLHIFFGTRKKSIGILHGRYNKQNNEGEFIFKKENTQLLILSTIITFISFFTLTILFLSQQNTRIYKSYFDESNTSYMVLFMTLFVIGLGILEEYFWRNFMIKTLPSGWIVALFVGLHFALLVMMITSSIVEWQYCVLLGVLSVVWHMVMVYARKLLKFSSVLVYGIAARFGIYTAFYILFLNIKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LEY4</accession>
    <name>A0A8S1LEY4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0390029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LEY4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8066320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="14817" checksum="4D161267B5504339" modified="2022-10-12" version="1">MFVCCGKKKKDRKHLSLGNLNQLNLICQDGLYFDKNLKIITEYNKTNVSEIGNKECKYYCPICFKYYDCMLRSTCCSNYVCHICAVQSLTNKMYNCHYCRNEHCKYVDVDPNQQLKIYTDSHYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFH0</accession>
    <name>A0A8S1LFH0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFH0"/>
    <dbReference type="OMA" id="CDFIEIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="60" mass="7160" checksum="31266A2B095CA39C" modified="2022-10-12" version="1">MNKLIEKKESKSLDLYVVLRNLEYLKDHLVISEKKINRKKIKIDDDLEKTILICNSDQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LK50</accession>
    <name>A0A8S1LK50_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0390160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LK50"/>
    <dbReference type="OMA" id="NQVCSCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="114" mass="13074" checksum="5BC5C90E3016AAF7" modified="2022-10-12" version="1">MMQNFRKIINVPSQIVEITKISKTKGDLEEIISPESDQEDLSCSTPQSNYFKGDNDSKTKKKKKHIRFSFQNQVCSCQLNSKLNSPKDSPRMNSQSIQSILKPQSLSMFCQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LMK7</accession>
    <name>A0A8S1LMK7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0410045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LMK7"/>
    <dbReference type="OMA" id="HENYEQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="164" mass="19792" checksum="EDC9FF3E5085D2F4" modified="2022-10-12" version="1">MFFPPPYYYKQFISPKSFPVPDLDKVRQHIDDYYSFGEFHQFKDYEADQLQILKEKLAEKNIHGNLKLKLKELSTEIFEICIKLQDSIANVDNQHIELKNQLHENYEQFNLICQFINMKQKYIDLEEIFKNELIKLNQINKDMAVKIQEFQKQIAELNSDFITY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPF7</accession>
    <name>A0A8S1LPF7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0420070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPF7"/>
    <dbReference type="OMA" id="FEEEFKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8068265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="157" mass="18957" checksum="4DFF91F32D72AFFC" modified="2022-10-12" version="1">MYQIYLTFLRDIDVELKKTLESSTQVVNDHILQRTCKQQDLEFVTKKLEDFKFQVDKYGIQAIEKFRENLKANIEGPIQAQTHFIAMKNQRQKSKSYKIDDLERVIDSRIQEASEKQKQQLIHYLDTFEEEFKFQNQEMANEYQQIEKELQELQLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQ38</accession>
    <name>A0A8S1LQ38_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420188</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQ38"/>
    <dbReference type="OMA" id="VENIMTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010378">
        <property type="entry name" value="TRAPPC13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13134">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13134:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="449" mass="52388" checksum="CD2DDDE05565AE5E" modified="2022-10-12" version="1">MNPKDFFEITHYSLQEIQQIPPFKNNLSTFNKQTYSISSAQTGKSHSLSKLINEPIYFINDGHLYLVGRKEIQQGKTYVGYSVQYLTLIKYKGLYEMDSITIETNLESLKKNKTYRQLSSTYFERIKDGQELLIFNESKLEEQDEYSLSYVLSYKIQNSAQLVQEQIKLEKKQQHTFKAFQPFESHYSMVSENFPEVFLEYQCLNVSSQPLEMKNIEFKVENIMTIQRLKDYQLPVVLGQDECLKIIYRILIQTSDLKAIYTVLTQKKDLTEYDLRLGQIHLEWGTCGNYFGTQKTCLVRIYPINLRPSQSIQIEKEVITTIEIDIELGILFDVPQIDLAIKLEELEMKAIKIVGMSQMKLNYPPGIRTRKIKLDVVGYNCGVHQIKGIKLYDPKTLKNISYKNFLSVFVVNKQQNQRAQQLNVQELNLLDLEINQPKIIVESNLLDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTK2</accession>
    <name>A0A8S1LTK2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0440128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTK2"/>
    <dbReference type="OMA" id="QYNIHNF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="309" mass="36087" checksum="16B10E85225F4BC2" modified="2022-10-12" version="1">MNNRIFRFQNTNILAQALTNKIHELEYQSKKGIKYPYGNNEEFIVIGQMIFEFYFSDYLIQRGYPNKDSQIKKPSDLVLLRQRLLNDKNLAEIAIQYNIHNFVQIGNQTALKNNPKILAETLKSIIAAQYFDSGFDLEHIREISQSIIKELLDKGQNIPIAELKQNAKSSFLEFINNYTNLIPKVSVEWKKDDNSINVYKVQLELDSLINISKTGINKRQTEQLVYQEALRQIKSKLINQSKLPKINQMQKNIQEKQSSEQSTLIYELDNSIMNDTQYFQFSEQHELSKSDVNESLSDKVEYLLEKLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTS2</accession>
    <name>A0A8S1LTS2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTS2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="51" mass="5899" checksum="5082E28DED961524" modified="2022-10-12" version="1">MNIETPITIKVSKTNSSNSNRFTLITEKIDFNDPKPLLRFKTLLAHQIIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LVP1</accession>
    <name>A0A8S1LVP1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0480212</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LVP1"/>
    <dbReference type="OMA" id="MFLTQDG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872:SF9">
        <property type="entry name" value="X-LINKED RETINITIS PIGMENTOSA GTPASE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="5"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="5">
        <location>
            <begin position="7"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="59"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="109"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="156"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="516"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="355"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="333"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="534"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8072100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="579" mass="66608" checksum="19D5A93EFE4FD3AD" modified="2022-10-12" version="1">MISESIVYVWGADNEGQLGLGKNRGSSQYYHIPKICHYKIGFIQVSCGKDHTALLTQNGHVYTMGCNEHNKLGHATKQSLYAPQKIEQLSKVIQVSCGYTHTACITSDGALFTWGDNTSGQLGVKQNSNLNKVNTLTNCIQVSCGNKHTLVLTDAQECFGFGLNDNSQLGIQRLKTVVEPTKIKLPSLQQICAGNLFSMFLTQDGIVYICGLGFGNLQKLNYKQKFIKIDGKSTPIGLTSTNELIFFELDEQLMNIKDFCMGELIGMCIGDKAYQWQFDPEQKRITQLKSIDQLYSKQLIHFSAGNSHLICIEQKGSSSFIESICSSTTTLRGKHEERSKSNYIEKQDGNQNQRIQQLEKQLEEKDRLIQQMKVDYQSKIQQMQQKQKEMEVQLQNKDKEIAKLTIELHRLQEDNQNKNSDSEILQCHVQEEISDDDENDVKVLKKQTSLPYQQPTLTYHHTSHSQLQNYSYQQQVKSPVKSIIQENQYEDSLQKNKFCKTDHDREAKYQSLEKQKKKEVLNERPQSLIREKSNNNNNEKTNLSTIKSRLTDLQKNKEQLEQRMRQFEDRLRENKLYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M052</accession>
    <name>A0A8S1M052_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0510217</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M052"/>
    <dbReference type="OMA" id="IFTELKW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="2">
        <location>
            <begin position="696"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8073740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1048" mass="124856" checksum="26E3BD8706B4146F" modified="2022-10-12" version="1">MNEIAYPQTQFQRYSSRQINKVTYYCQSLNAPDKDGLEKKNQIYKWLRNLPMQQLEQVLSFTSYYRVHSFLKMFKQDQNLLHEYLELEQSPFEVNVKIKLNQYYYVRECNRIDMTNQWRYAQQYILDHTYISDYEWLFDTITIKGDIEEIIQSFEILSKQSLFTKNWRIETNEDEEYSEINFDSQQKKKAFRTLSEHIVNEMEKAILIKFQQFEQKCTYTKQDQLFNFHQIELNFNNINYEIETQEFQKVLEQSQVEMQFIDEKVLLQSWNQHIKWENLNLNNIEQQIQHLLNDKDLIFQSPLKYTITESLLFCKYLICKQLKILYPLKKPLEENLKEKKKPKQKVSNLKMFQGKVDQLKQINTYFTSSEKSKILPENEQRLFSTNLEEKEQQIYEHSTSFMKKLIETVLTELENYKQYKKKLKKVKNAKKSQIIETVQPEPTVQVSQDIIKEDEWNDKPKSKKQRKKQLEKQRKKDNLERKKLKQMNQSQQQSVSQDEKIEKSSKSSIKDDNEDIENQIDNYYDNNNNDELIFEQQQQNEIIETVIQDIPIITNSVLDDESDYIEVQNKKQQKKQSKSKTSRHKKQTNKKDKEFQDNQSLPDEQQYIPSLKKSTSQSVQKNNNHQYDELKLERMQSQQQNRTILQKKENAINKIQRSGLVKSKAQQKEQKLYTYDLDQSQLQKIYKTILEQKITNDVKQIYQKEIDQFNKYKAARDISIQRVQHVIKSYFKNCDTEIFGSSTTGLALKDSDVDMVVYGLQVYTKQQLFEPMKRLIEIFTELKWAVQCKHIFQASVPLIKVLVDPSIDFLSFKGEPKYIIMQCRNLDLNLKYGDPSQNIFIDITFELAPPYAIYNPYVQAFNIGFQSTQYTIDICEKIQGFSEVAIYLKKLLKIKDLNDSYTGGISSFCLTIMLAAIGQDHSIGQKLINFLHKYGCNFDPNKWAIYLDEKGQNNFYNIEDEQSNQPLTIISPINLQKIQINVTKIQTILQLFQQLYVEIMQNIDQIESCLQKRINQKELLENYNSHQISKLVDLVSWQCPNLLESQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M622</accession>
    <name>A0A8S1M622_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0550036</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M622"/>
    <dbReference type="OMA" id="KCCDFSK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007783">
        <property type="entry name" value="eIF3d"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12399">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12399:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05091">
        <property type="entry name" value="eIF-3_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016281">
        <property type="entry name" value="EIF-3_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8075730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="515" mass="60148" checksum="A24CF216BB8BEDE4" modified="2022-10-12" version="1">MDSDLQNLIKNYAPLSDFCPDPNYQFEEFKDRLFQPFSRQEKISRCCDFSKQTQVQKDLEDEYETVGQKVVKKPVQKKIGFVPQQQQQVVYQSYYQTGKKSKMSQAAKRVMVYKKCRFKDSISIKTDWISIHETQKQNIEKVQYSMIDIKEIQSIGTIKKYNKEFDKVRPNYEKKISIIGGEVISGSVQDDKYFKQLIAERQGTEEIPTIYTTDKLLFAVQTLKYSIYPWDILVTKKGNSYIFDKCPQNRSELTYLELQTINENITVDMPEDEKTVRNYCEESTIAQLSWQLLSTLNQPVLFQNSLQNDEEAEQNDQAEKYLFSDDLCFKYLEVSVKDRIDKAAKGEDVRAGQERFKVVVRTTVEAENNDGQPVLVKSLNECETPPDWKKSLLSQAGITTNTAQVYNTNNIAKWLCQAQLLECEEIKVGYLARQNQKDQDKHSLLLVETFTQRDLAAIINFKSYEIWQSVRYLVDYLKTQEDGVYVLLKQAYKQSVRIFNVRDNDELQEEDEDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M7Q8</accession>
    <name>A0A8S1M7Q8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0550091</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M7Q8"/>
    <dbReference type="OMA" id="CSKPNQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCC1-like" evidence="3">
        <location>
            <begin position="52"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="75"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="133"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="186"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="239"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="289"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8075819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="432" mass="48152" checksum="9E84D862DF58CC5B" modified="2022-10-12" version="1">MLQESKQNRVCVPTQSAMQEQIQYVTCGAESTFCITIQRKVFMWGQGIQIPQQLDLQEIEQIAASKEHWFAWSDTQVFGCGSNKQGKLGMNNSVTTPTLFDIFSEASQYGWQLQKIAVGLDHSVALIEVEDENKIFVWGSNTYQQLGFDASEQFVAIPHQLDLDNLPRIVDIYAQHNYTMAIDEEGRLFSWGSNEFGRLGQNAFAKCMKLPSPISSFGNIKIAKLALGTFHVLAIDTQGNLYSWGRGLQGQLGHGNSNDCSKPNQINSLQSIKDIACGEAHSIALQTNGSIYVFGSGQFGQLGLGDYKQQDTPQILQINGEMIACGRHHSAVLSKNGALYMFGNNEQYQLGLECGKSGAYSTPLPKQSPMNTNQIFELTDEFMNKIEASRSEDLEQLLVEYQQNTSQIPNTQTTIFIKDLMERVIALLKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M872</accession>
    <name>A0A8S1M872_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0550173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="212" mass="24242" checksum="4319BFC8C6C424D8" modified="2022-10-12" version="1">MKFKCXKLNKTQNEIYKNYIAKRDSNGSKTYRDSRDLDIRIKLNNKSTSTQQALLQTLINKSNEKKSVEPEDSPNLCSTPQLTMLQSVIQNSLLLRSGSQGNVACTSKLNTKLSESKLYKIKNYLEQAKTHRPITDSYKLSQEINQIKKTKLVTVERNKLLSILKIALRAKTKIDQFNEYLKQQEKISSVIQSLNLNELSSENSVESILSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAN6</accession>
    <name>A0A8S1MAN6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0570042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAN6"/>
    <dbReference type="OMA" id="NQWSQMA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="65"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="128"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="191"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="191"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8076759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="475" mass="56173" checksum="999005E7ABBABAF0" modified="2022-10-12" version="1">MQFIDSYLMDDFHQKFLNFFSEVKGIKNIRFNCIRDEFNINIDTFPEDQEVFNIQQANKLNLQSQPNKPKKNWTDDDKKVLIWLVGKWVAQNQRDIDLISDSDWNVIASMMPRRDSFACKQKWLQMFKLPLQQAPWTPVEDNILQTIIQDFENQNKGNQWSQMAQILNKINNQQVHRNGKQCRERWNNHLNPNINRNPWQLSEDLDLMCQAKKLGKKWALISKKLKIARSENNVKNRFNCLIKKERNNKSGQNLLIIRKKKEKDEINSQKSSISNLQSSEELSLEEIKLINIIIKKIECQRKEEVKEENSKLLKKVKGEQQKEFYKEFKEKIQHFKNMKSLQINVIDSNLKDDEITTLMPCLINQGNHQIYFSTPEQFYIYLNQSSETNQLGIQSENYFSIPNGQGSFMYDHKVFQSNAMLQSREYYQGQHGIFNQRSQVNQNHVGYCSSINSLPYQIGLSPYPSHAILTYQLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBW0</accession>
    <name>A0A8S1MBW0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0500001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBW0"/>
    <dbReference type="OMA" id="QGALLCD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="120"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="154"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="188"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="222"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="256"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="290"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="324"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="358"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="392"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="426"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="460"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="494"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="528"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="562"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="596"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="630"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="664"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8072744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="749" mass="88260" checksum="06766CC75CC354FD" modified="2022-10-12" version="1">MNFIKLNEESVRESNKNLSINLCINNFYSLERIHNFNRSSQQQKAQQMIQGKKLLNQIINWNQNKNIKGLWIFQNQQKKIKLVKNIIYLNQSSNVKLNKKLKKSDVLNEQKKDKFQLIVEYLDFIKAIALFKMNRFEEALEYYDSAIQKNPENADFYKGKAIALKKMNRFEEALEYYDSAIQKNPENADFYKGKAIALKKMNRFEEALEYYDSAIQKNPENADFYKGKAIALKKMNRFEEALEYYDSAIQKNPENADFYKGKAIALKKMNRFEEALEYYDSAIQKNPENADFYKGKAIALKKMNRFEEALEYYDSAIQKNPENADFYKGKAIALKKMNRFEEALEYYDSAIQKNPENAEYYKGKAIALKKMNRFEEALEYYDSAIQKNPENADFYKGKAIALKKMNRFEEALEYYDSAIQKNPENADFYKGKAIALKKMNRFEEALEYYDSAIQKNPENADFYKGKAIALKKMNRFEEALEYYDSAIQKNPENADFYKGKAIALKKMNRFEEALEYYDSAIQKNPENADFYKGKAIALKKMNRFEEALEYYDSAIQKNPENADFYKGKAIALKKMNRFEEALEYYDSAIQKNPENADFYNGKAIALKKMNRFEEALEYYDSAIQKNPENADFYNGKAIALFKMNRFEEALEYYDSAIQKNPENADFYNGKAIALFKMNRFEEALEYYDSAIQKNPEKAEYYQGKANTLYLLRNFDSAQKYCDYAIQRDNNNPNYKLFQSLLQISKHNKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDH0</accession>
    <name>A0A8S1MDH0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0560053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDH0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="26613" checksum="CDBAEB68E59C1CDA" modified="2022-10-12" version="1">MKELEVNSDQDAKYQIMLYKQRQEQIQNASKFELVFNNNLSNCYEELSHISEQKLKLLGKDNDPSKVQFLKFEDQVQTNFHGQSKSFTQDLNSPTKKNLKFFSKLDPVEIENQMNRMKEFKQETDILKFFSSFNNQYDKEVQIFIYIFQKVNQCNLDYPTEKDRYFSMDFQRGFLPKTIHRRKRAKKKSTKIVINTYRVDPKKLDQVQAQTEKIKQVGQFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDI0</accession>
    <name>A0A8S1MDI0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0560058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDI0"/>
    <dbReference type="OMA" id="CSYSDNA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="111" mass="13001" checksum="AC131F621890B39C" modified="2022-10-12" version="1">MDKFETIQGKLFYTVYLMHKQNEISEEQKGRIKVQWDPQIQEISKDLNRLRQNFLEMACSYSDNADSKITKITPRSLSRLNVRIGHSNFKLANKSYSSLSSPIDHRKPLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEM5</accession>
    <name>A0A8S1MEM5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0590094</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEM5"/>
    <dbReference type="OMA" id="WCQQFED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF106">
        <property type="entry name" value="NIMA-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="166"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="241"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8077959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1255" mass="147412" checksum="C201E5AAF405F066" modified="2022-10-12" version="1">MDYLLNNIEVEIDLDQSDDQSNQICFKVYNKNLQTFWMVEHKSEEIQRFGLNVFNTQMPEESSERFQWLLELCYKINKSQNNDYSILEFFKSKPIQMKRKQSKKRSLSTFSQLLKMSCVEETRTHSRNTVTAKQKQYTQIILENSSRFCDLKTTRDFIDLLTLLHYRDIKKVGSGAQSQAFKALNQQEHLVAVKISQNTQENLYAALMQRQINQDVAENFVGNYLFQYQILEVEDILIIEEELAQNTLEDLIDRKQNINENFTEYEILQIIKDIVDQLFNLHIGRQSAHNDIKPENILIQDGKFILHDFGAIRPVPKDQNQVEINQIFGTILYMSPLKFEHYLSTNQKQQLDIHITHTIDSISNNFKHNPFKSDIYSLGLVLLQLLLHNDKINLRKQIECLFYEQRQQNLPGLIEKYEIYLYPEKFFLFDQKLKRIKPLLKLLLDENEETRISTIELATLFNSQICTSIQTSFTQQFVPTIWKSEQGGTCQLAYSLKSQIYQGVYNKEKQEREGFGIMYKASKNDLIFKIQRHKKDKKSIYDEDQDFEISQVTQLQESKHILSAKQSLVQNQLQILYSGHWLKNLPHGKGELHIYNTVSDLNYKEVNIDYGKLINKIDDHHEEHQLQINSQFYFGILDQGTTINGLQVQFFLNISERLAEDEFIVGKNYYKFEYDAITQKYYGYQQPYDFTVIDVLSLNDKEIICLIISRFIRNLKSKISHFEIRRYVIEKQIFLKDNNNNYIYIRFDGQHYKDSHQKQQKEQQNRKPIQPQIIQKKSTTACCVKQNKKINQNQQKEIEIQSDVLESQIVLECLNQQKSNVNELILQTTSLSSHALEWLFADDIISQIEILSFSNSLIKDNQLNLILMSQSVTQVRDLDLSYTQLSVQTITALINSENLNKLHKLRMAGCQWCQQFEDFITCDKSQKLILLDISNNKWVDSNLLKLFTPVYLPNLSQLNLNKTSLTDKDLKKFLETPLGKKLNLMPQTSKQWEQPIIDFKISIRATNSLFENIRNLEKYGYMSQHDMEENNYIKDETLKRIAHSQGLQNIEYLNLKSQDITHYGMKHFLQSQFITNIISINLSNTKINGDVLKQIYSSKISHLKILKLKNCNNIQFDDLQLFLKNYKAAYLQTFYISSKQISTKKLVTELKNLQSLTQTQIIFNDQEKQTDISNLLTAILTWVAILISKQKLQKLTLDLRDCKGINNGFDLFLEEIQQLKGEPYCTLIFKISNSSMPVSIINQLEQNYKSIQFVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MF00</accession>
    <name>A0A8S1MF00_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0570143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MF00"/>
    <dbReference type="OMA" id="RINFEHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="76"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="222"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="379" mass="46142" checksum="339243301C4510E6" modified="2022-10-12" version="1">MYKIIQPSQEDKIYFGQKQVQQFQQDQEESIYDDISQIPTQLQKEELEQMLDLMPHFGNSKFSQNQEKNLDLWKSIQQIEMRIKEQENNFKRINFEHQEEIKKLKNELNQQKHLYETKIDEIKQSRIQSSNSQIHSPYSTENNREYNYRSNNNYTEVSQEKNYLQQLVQDLKQQIQVLQTKSFSTPYQNDVIWQQKYKQIEQQDKSNIERISHLETQIINQKLFYENDTSKLKKQLDEKNQEEIMLRNQIQQLLQDLNNYKNKFNQVSQTLQQQSQQYQSQIYQLQLQTQKNYSFENKNQNQQLLELTEQLQASTILLEKKIQECEFYKQKFLQQQTIQQNQYIYSHRQSLTPTTQRIQTQNPTVFPISNKQQLFQQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNK8</accession>
    <name>A0A8S1MNK8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0660181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNK8"/>
    <dbReference type="OMA" id="CKKFSNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8081810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="63000" checksum="50A37E673D235C59" modified="2022-10-12" version="1">MQFAVTKLPQLCKGVLPQKFEQLIYLDLVISSINQIKKADKDLLILSSKHFYLITNYYELCTYEEMKAELKKLKIEDLKNILLNNAQEILKSKQNSVANFVSIIPVLSRNFNLNEFSQLIEEQLKEMIEIVKDRHECKKFSNITLTRFCKSMGYILRNSDNQSLKQHYQTFIFQIIINKPNTQLRHIYSMLHYLQFIKYNQIISYLNQKQLATEVSKFILQRFRTQLNFEPDISENPEFYSQLSSFLFKTNYFPYDDALFIEETLLQKVKFKEEKVEFAYLKLLFCQLSKVGVGEKLIKRMLQIHKKMQMEKEFLPYYLLSLLYFQTSHIMDQFRINPTKENMERLIKEPHYIQPLIDQAIDAIRQFGYAQVAYPQESINQTKICQAILNCFNIKLFEEENIKAQFNQNNSQTGRTNEINNDVEDFLQSIGIEFIKEYNEGFYSYDYWIPSLNIIIECDGSFHYQTNSYDKKDSSTLARDFLILNSNRKLISINWFKQREKRFTEDKFGYISQLWNKLDKNPDIIYAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSF8</accession>
    <name>A0A8S1MSF8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0590037</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSF8"/>
    <dbReference type="OMA" id="LNVMIID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015671">
        <property type="term" value="P:oxygen transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00454">
        <property type="entry name" value="TrHb1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002779">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019795">
        <property type="entry name" value="Globin_bac-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01213">
        <property type="entry name" value="GLOBIN_FAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="15"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8077864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="542" mass="62512" checksum="857E4EECBA696F9C" modified="2022-10-12" version="1">MQAEIKNIPLNQGKYTTQQRLGSGSFGEIYLVISQSNEILAAKFEEAKSKHPQLVFEAKVTKALQGGIGIPVLHWFGQEAGLNVMVIDLLGPNLEDLFNLCQRKFSLKTVIMLADQMIQRVEYMHSKSFIHRDIKPDNFLIGLGRKSNTVYILDFGLSKKYRDAKTHQHIPYRENKNLTGTARYASINAHLGIEQSRRDDLEAIGYVMVYLLRSYLPWQGIKANNKQEKYHKIMEKKMTTPVEVLCKTLPIEFSTYLNYCRSLRFEDKPDYSFLRKMFKELFQKHGYDWDYQYDWCLAINGQKTNTYNNGKITIQVNANQYDADENQPPITIQINEKVQDKQVVESKIIENKYQRVQDPDNDIDQTEQIARNLMGNEQFNKQQEQIKKNLLGDSEIKLSIPNNTNQKNQAPIETVFNPRVVIQKKTIYERLGQSMAIDFTVDKLLQKLQNDPKLKSYFVKTDMKKLNSQMQAFLTMLFGGPSNYMGRDMLEAHRGLGIDDTHFNLVVKHLSDTLVNLALPEDIVAEVVEVCESLRDDVLGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZ91</accession>
    <name>A0A8S1MZ91_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZ91"/>
    <dbReference type="OMA" id="IILYQQD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="70"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="364" mass="43697" checksum="2E570F90B9D58978" modified="2022-10-12" version="1">MDLSDLFQHSNQNFLLINQFQNRTSQTKESIQQNIQSLLQFKYRNIVNEGDQLIDVTDQLQNILNYSEVLQKKFEQSQKLQQKLRDIEEELQSYFQCKQNFIEIIEEYKQSLEKYKEMKQNVKEFSQNDLPRQDAQNRLQEYASLIKKLKDYSTFKNELNDLKKIDKRFQILEQEEDYLEYYLQVNNKLQEQITLSDTQLLCHFVQMLGQSISYLKSHQNCSVIIYYEDKKNISEEVLMQYKYAILLKIYDMVPTLILIDKENSDAQVICASQHRQQNKLYLENYKNYLSNIKPSYTLLKPDIILGSTFTKIIEKLIQINMINQIRNITYQEMLENTKKILLYQQGLEQLDCFQFLDTMQIELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0Q4</accession>
    <name>A0A8S1N0Q4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0690046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8082973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0Q4"/>
    <dbReference type="OMA" id="ACKSQIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19774">
        <property type="entry name" value="Bbox2_TRIM23_C-IX_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="386" mass="43761" checksum="C37201EAA3DBE636" modified="2022-10-12" version="1">MNYFACPIHNGHFLSLVCTEKSCNENPLICPLCEHADHQSHNTLPIKLMLQQVDDAVNNNCDVTQQSIMDVSIFLDKSYNECVRTVNRFNEQMQNISKQLAKKVETYYLDLKNQLRNFSLSKTPIHFLLDSLNIPVELLEYKKNIEQILEQYTFGNGIAVRQSINSAIMIACKSQITQLNDSLHDIQVEFDNKVETLSNIFKAQPVTATDQVQSIAFQFSQKFKGANIGLSGQNMIAEQKTSESNGQRFIICEPCLPKNGVYKFGFKIIKYAGWIGVGVCHRDIIASANYKFNYTNIGHGSYLISNNAYSWSHLQKDLNSAHKSFDFGVGDIVVIEVDIPKKKITWWKKKSQLKFSMNLDTNLDLYPCANLCSPGDTVEIVNKVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2C8</accession>
    <name>A0A8S1N2C8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0780174</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2C8"/>
    <dbReference type="OMA" id="HAGPDCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001073">
        <property type="entry name" value="Long-flagella protein kinase, CMGC RCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000271">
        <property type="entry name" value="MAPK/MAK/MRK overlapping kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8087387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="389" mass="45069" checksum="11BA977ADAEA846B" modified="2022-10-12" version="1">MNQYYILVKIGEGTFSEVLKAKSMITGQLVAIKCMKNRFESVDQVKNLKEIQALHQLQQHLNIVKLHEVLYDEPSGRLALVCELMEMNLYDCIKNRNSYLSMAKVKKYMHQVLKALDYMHKRNFFHRDIKPENILIKNDNVKVADLGSCKGIHSTHPYTEYISTRWYRAPECLMTDGYYDQKMDLWGVGCVMFEIIALLPLFQGENELDQINKIFKILGTPEPELLNRFKSQASHMEFNFKPQKGIGLERLVPPHAGSDCIDLLYKLLQLDPIKRISAEEALRHEFFEEFWDAPMSQSSDSTQFGKAINITKPKKRLNKGIYPGTIKLEVKMESKLNQSDDENEKVRDGLLYPKLPPIGKPYIKPKVKNPYAKKKLYDDYIIVGKKALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2E1</accession>
    <name>A0A8S1N2E1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pyruvate dehydrogenase E1 component subunit beta</fullName>
            <ecNumber evidence="4">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0780184</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2E1"/>
    <dbReference type="OMA" id="LPLDTCF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027110">
        <property type="entry name" value="PDHB_mito-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006667">
        <property type="entry name" value="PRK09212.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008854">
        <property type="entry name" value="PRK11892.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624">
        <property type="entry name" value="DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624:SF96">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="5">
        <location>
            <begin position="32"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8087407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="360" mass="39779" checksum="127F12C2424EA010" modified="2022-10-12" version="1">MYKHLLKLRYAYQPSIKFTHAHPSSQYTPIQMTVREAINLAMDEELAHDPNVFLLGEEVGQYQGAYKVSKGLFQKYGGDRVIDTPITEAGFTGIAVGAALYGLKPIVEFMTWNFAMQAIDHIINSAAKAHYMSAGDQKASIVFRGINGATAYVAAQHSQCFASWYSNVPGLVVLAPYDCDDAKSLLKAAVRNPNPVVFLENEILYSESFELSAEARDPNYLAPIGKAKIMRQGDHVTIVAFSKMVQYSLRAAEQLFREGINCEVINLRSLRPLDRETILESVKKTGRVVCVEEGWPQSGISAEITALIMEGGAFKYLDAPIQRVTGVEIPTPYAFNLEAMSFPKTEQIVDAVLNVLKGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N534</accession>
    <name>A0A8S1N534_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0730018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8084846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N534"/>
    <dbReference type="OMA" id="FWKEFTD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052603">
        <property type="entry name" value="EFCB6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20875">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20875:SF0">
        <property type="entry name" value="GH12158P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="399"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="509"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="926" mass="108692" checksum="77843B9589015703" modified="2022-10-12" version="1">MATALVSAGQSIIGTYVQQDIETRLQQDVYKKQIRVREFFTDFDRLRKGWVTEDKFRSALSMINFHFTKDDIEEIIRRYKLNDGLVQYTTFCNKLEEQFLNNEAKAQVFQAPQIFNQDEEDTVKRLMQAIKRKIATKRIFLKQPFQDFDRTACSHITIDQFSRVLNQLGLLPKDQYLQLLIRQYIDNGNPKEVNYVKFCDDVDNVQEMLSGVITGIKQNPKDVHPDDDFIEDKEGLDLISTLFTSKKLTDNINTLNQVLKKIQGDVVMKRIRIREFFKDFDPLRKGLVTESQFARILHIQNIPVSEKEIQLLLNQYKIDKIPNGQVDYNQFCEDVDKIFTIKGIDKEPQAQVPQIDDSTTLPARRRYLQMTEQEAIQLDELLMKYKQAIQNKRVLLKPVFEDFDKTKQGYITTNQFLRILNQFNLFPDPISLNLLLKRFVDKANLNEVNYYDFCRIVDQSDEGVAISRSHADAFKNYVKSDNQSQAFIRNDQPNDFEDLMAKLRRIVKEQRQRIAEFLRDFDKLRSGTITITQLRKGLSMAKILLSDAEFQLILNNFGCKDKQGFVFWKEFTDQVDQVFTTKNLEKVSPSEDVPQMSTQYNYGRVSITERDRQVAEVVKKKFQYFCKATRLDIKQFFQDWDKLGRNKVSPKQFRQTLATVNFILSDEEFQAVVKIYAAEDDGDIRYVQFINDTQPPLEILTESGASQAYVGVKPKEKEKLQPSVLLEQIKVAVKVKRLRLGDYFKDFDPLRKGMMPTNKFRGVLSQMKIDLDQESLDLLETMYVVPEDPIRVNYAKFIEDVEIVFTKTGLDKDPLMKPPVHIIPTFLDPRDALTQDEEEALHAIMLRLGEVVRKHRILLKPHFQDKDKTKSGKITFTRFRSIMDFHKLPLTDDQFRVICKRFAYQGIEFNYVEFDEILKKYENFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5R1</accession>
    <name>A0A8S1N5R1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0670222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5R1"/>
    <dbReference type="OMA" id="EFNQQPK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="300" mass="35472" checksum="226B9C266B863D20" modified="2022-10-12" version="1">MEQNTKVKQEYFKKLKLIQKKMENIKADQSPSVIKYMVRRKSAEEVQAPLHDVEFNQQPKKKKLQFQKTFQKVVIDPIIKLDEITEEKWAIMSNNYKSLIEKSRQSISKHSQIFQPSLVTKEHHKFQFLTRRQSIEFRLREITTAPSNHSSASPIKINTNTQLADPNYDRFKKYYQKIGIVADNPVQMKFKQLISTPIAPQKRKEKSQHSFNNSQQNSSSSPPPKTRTIHIKPNRNSQYLQEIKEICSVASNYQTHVKQDERIQIHQTSKQMGYIQAEFNKFHNMLTTDLERLDFYDEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N932</accession>
    <name>A0A8S1N932_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0830134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N932"/>
    <dbReference type="OMA" id="NMRIDAI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="282" mass="33977" checksum="84EF3CC3DA3DE4C3" modified="2022-10-12" version="1">MNRLLITQTGEQLRNEMKQQAYQQRTEKQVKKKEQRLPELVRQPTNLLIDTERDHILYQKTLSEVALSNLNRVASLSLLGQKSKNKLETHIREELNLPNNFQYLNQSLDKQLNKILSDKDLKTKRKRHLLTELKRIEKKRTTSHNEDFSIFSSCIEDYDKNQKMTAIINAEGKLAQLTRRYKQKELNDQKLKLNLNISLEKMQERKNMRIEAINPLSPTQIKINSQRQFDEMFYDNSEERFHNRLQEKHVKQICQIWNQYQFPKQTKRWIQFIQNAQQKKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBE7</accession>
    <name>A0A8S1NBE7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0790040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBE7"/>
    <dbReference type="OMA" id="INDSAYA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035895598" evidence="2">
        <location>
            <begin position="21"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8087646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="357" mass="40581" checksum="BB74F0148478B3DF" modified="2022-10-12" version="1">MKSIILIALIAVAFTARVQERNMAKVQADLAKSTYGKALLHLVELHSMAGGPVQELIDAIEELINDLEEELEELEFNFKVRTNEHNALVTSLEQDIQDAVIDVNNTQDTLDNLLYPRRAQIQSKIDSVVGYQEENRKNYDEAILVREQEHEAFEAQVAELNDATASVDDALALLASLTNPSLLQIRRFQNTLKNIENKIRSRSRMAPMIKALINLASNQNFSNQDVLKNIVDALNEFRNAVVDQINDLTAQEAQDVVDHEAYLEQLDDEYSEFQRQIDRATVDLTATNEKIDQMTEFRDQRAADQKQYTAELELENNTYAEETDIYTNTKNEFTRELGVSEQALALVKSVDFSNIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCZ9</accession>
    <name>A0A8S1NCZ9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0830004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCZ9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="360" mass="42126" checksum="F77387D61D535122" modified="2022-10-12" version="1">MVLQLQTQYIKWINLSLLNITKQVINYGDQKLNNHELAFFCGIQNMDVVKILNFEPDYYFDYYNCIMIEILAFQFVVIKQLLEKNVMMVIMILLMDIQIVNINVKSILIIVQILLFKQKSIYRQSSYKIKISEYKLAYDLDVISLSCKFKFCICIKTLCLQCNNDTTIYLRFFVEILSIKNIQEYLFLISINGDLFILICGDALINPIVEECDVQSGDGYINYKKQNVYVCGKQHCSTGKICDKECAQCLSLDLINFIFKSYIDGYYNIEDKCILCDSNFIRFKLSSNLCTSCYRSDCDQFLVFLQIKKLSIVIPFFVMELQLNHMGSGMIINKIIEMEFSDNEINRMKDINPQRIILMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFH5</accession>
    <name>A0A8S1NFH5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFH5"/>
    <dbReference type="OMA" id="VIMMAAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002893">
        <property type="entry name" value="Rab_B04 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="218" mass="24722" checksum="8D376A9FDDE4E40B" modified="2022-10-12" version="1">MSSPDKSPLFKFIIIGDAGVGKSCLLMRYMKDDFTTEYNVTIGVEFLSKIVNIDENTKVKLQIWDTAGQESFRSVVRSFYRKVAAVFLVYSITNKQSLERLDSWLKEAKDHSSPSIITVLVGAQNDRESEREVSYEEGKYWMETNGLNLFFETSSKTRENVDEAFAETAKLVFLNHINGGANNEAKLPALNQTDLSVKKLHQPLEKSNQKNDENKSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFM8</accession>
    <name>A0A8S1NFM8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0850097</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFM8"/>
    <dbReference type="OMA" id="NRQWQIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="322"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8090099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="551" mass="66298" checksum="B3EE0AD2B881D245" modified="2022-10-12" version="1">MQSPLRLYSASRAFTCRNTETKPSFFISKKAKQSKKQKEIDNLNEQLLLVKQSNNLLFEENKQLKQQILHFEKQLNKQEKLLILNKQSKAESKHSDFDLQIRMKTEETCSIIAYQKQIKDYKARDQYYQQQIEELKRDTRRTKLQECQIECKVIKEEYDKMKFKYQALIHQIQFSQKDELLRDNIKISQELLITQQKLQELQLQNKKIQQELHQTDFKRIRSEKLVFDKERELKLIQFEYSNPKKTINDLNRQWQIRLENQKLLQQQLEDELRLRNEKINLLEKTLKEDKAAYQLKEAFYKREIDSLNIQIGQLKETVQHLEKEQMVEQSSDEQESQSEKSVRQTSQSYQKQKTQLLMSRKKIQKVNSTEVEIIGKEIALKFQLAQIPFQRLDSYIYSKSSRGIINLSEINDILKQSPFDLPDDQSLLVARFLAEPEQEEWIYYDSYQTNDVVIVISIFRNLVKPFELLNPQLYDSIHQELKQIFKLNKNKLTDYLMLNGDICDLTYFKKAFEYCDIPLSQEQETYIAIKIYEKYKRIVNLKYQEIIDYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGD8</accession>
    <name>A0A8S1NGD8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0750062</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGD8"/>
    <dbReference type="OMA" id="CRENKKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8085774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="125" mass="15142" checksum="E253CA21F15A4200" modified="2022-10-12" version="1">MQQQQQQQQQPRARTKERYVCEAMNLVKLWRQIYETETKVIDGRTVRITLDQAAELVGCPRKTLEDYYYLLKKAQNLINLEDKKNEKMGFIRKICRENKKHQQLLKQEVEFNNINQFQLDEIHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NLC1</accession>
    <name>A0A8S1NLC1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0920030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8092920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NLC1"/>
    <dbReference type="OMA" id="NRIAKQW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37028:SF4">
        <property type="entry name" value="ALMS MOTIF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37028">
        <property type="entry name" value="UNNAMED PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="388" mass="45778" checksum="0F9B63C22CA18D95" modified="2022-10-12" version="1">MDQFLNDFEQYCRNIGILEQESNFEDKEDELGQILFKHILKLNKESLMQLSNRIAKQWLDQQQSQKIVQPNSAKQNNSKRSKHSQSTPQESMKSIINSNIQSTNYLYDQYIQKEENKILKSQKIIEEQLKECTFQPQILKKSQQLDTNIPVHDRLAKFGTDQRLKQQISSEIKNKNDLKQCTFKPQVNHKVSKTLEGDPFSRLYLNALSQRQSKPQSQEKIYSHKPQLISQPPQQQLGYLTIPVEQRLYNNFFDQQQQLVQQQEIEKLAELEECTFTPIINQYASISNGIDNKSKVFERLYNKSATQKSNSEIKEQTQILNRFSSSKQSEQIIRNASFDKSKYVSQFQIEQSPYDRLHEEYKRIQKKKKIIENQVFSSIPFKPKINKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQ65</accession>
    <name>A0A8S1NQ65_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0960105</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTH2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQ65"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003314">
        <property type="entry name" value="PRK04322.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8094608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="196" mass="21657" checksum="4B753EC7BFC20F1E" modified="2022-10-12" version="1">MDQSKQLIKNELLNLSFSEEQIAQVLPSADSVEQAVELLLALQEGQQIKKQNIGLFTDIDSIPFDINFDDIKPPGEHFDKRYKMVCVVRMDLKMGIGKIAAQTGHAVLGAYKQLLNDKQDLLQKWEGSGQAKVVVKCDSQQELHNIEAKARQNGLITYIVTDAGRTQVEPGSQTVCAIGPADCDLIDQVTGHLKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NR93</accession>
    <name>A0A8S1NR93_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0960036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NR93"/>
    <dbReference type="OMA" id="QEVRCEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="69"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="162"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="37936" checksum="341C59BBCC729385" modified="2022-10-12" version="1">MEYRLPVQPNRDIPQSIAQIQQEQRMKFQLQQIQAKIIEQRPNFQEMQGGQSPNKPWLGEDIYMVVFLLTVENDRLREENSQIQQLLNTSEIRYKGVDLVENEVISQRIKIGEYEKKLAQLQMECEQWRVKYQNRDKENEDQRYQNELQRRIQVDREIRELTARFIADRNQLEQENRSLRNELDQIKLSKYSFEDHKKLSEQLQKENQKLKQELDTLKKGFDELEYVLQTAADLEKENQQLKIQIDTLQKQIQQLQQQQQQQQQQIPQRPQSSNRIELSNSNYNVLQENKRLKDENDRLRTLLNLGTGSYSKPFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NSE8</accession>
    <name>A0A8S1NSE8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4200 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0920058</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8092976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NSE8"/>
    <dbReference type="OMA" id="QENINTY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051147">
        <property type="entry name" value="CFAP_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025252">
        <property type="entry name" value="DUF4200"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683:SF3">
        <property type="entry name" value="CILIA AND FLAGELLA ASSOCIATED PROTEIN 100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 42 LIKE-2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13863">
        <property type="entry name" value="DUF4200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4200" evidence="4">
        <location>
            <begin position="159"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="27"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="493"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="312"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="493"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="534"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="545"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="572"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8092976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="593" mass="71811" checksum="D074DBAC83285744" modified="2022-10-12" version="1">MNQAQQQTQDENPFQFELDSKKFLQKLEKERNSGYNDDDEEQKQVPHTQKLYQTSAKSTRFGLLQKLEDQTNKLAQERDEMFANEAKMRDPNQPQKIDEIAQHIKHFNPKLMEYRDDEARKTIYGKEDKKKDYELQEMKKKKYRIKSILREKRETITDFIEKKREICLANLNIMTKKEETERLEDFIKNEQESLRARRLYFKNDCELVKKFMNEVKFQADQAAYQADREAKKKDEVKTEVAKILAQIDRLKLQKTKYGEEFEKLDKYRQFLEKIKDTYKTKFSEFEKKDINLFSNLQNEQKNQFFVTGVDQREEQKQTQEQIEAERQQERMADLVIEILNNIEEGNLRNIQNQRDAEEDIEQKKRELEKLEQKLKNEQDEHLEMLKAEERKLKAQLKIQNQLKDQEKVDQTKLQFAEGEDLDMDKIGKKIIEIQQEATKNQDILGKRLQIDSKVIKQVINQLEKIVIDLSEQKLQFLLRTKTDFIEAEKKIKKEKQQQRLNNQKDEEKKKQIMERRNKKEEHLQKFYKGRHDMKRNYKQEKPVIETEETETDQNDDEKYLRESYELVYVPPQQKSHNQQQTNEQNIKQNDRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NT01</accession>
    <name>A0A8S1NT01_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0980134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NT01"/>
    <dbReference type="OMA" id="HGQKQHR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8095362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="93" mass="10826" checksum="B6919615C827824C" modified="2022-10-12" version="1">MSESNYYQQFGINQSVFNSESPKKLSPILKVSHSQSDGDIDIRKDSYGNIIKHGEKKHRIQFKQKNEVFIVENWKQYNTDMANQESPCLCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTY7</accession>
    <name>A0A8S1NTY7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tubulin/FtsZ GTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0990119</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTY7"/>
    <dbReference type="OMA" id="SCCHSEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="5">
        <location>
            <begin position="46"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8095722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="426" mass="49578" checksum="80554C1D19058AC6" modified="2022-10-12" version="1">MQEIIIIGIGQSGINIGLEFIKQLNYDHCLNNHQEIINQDRYNKKINVFYNENSRQQFIPRCLFLDLESSQIDKLLLQKDVIIDPNCCFSMNCGSGNLFAIGKYTEGAELMDKCKHVLNKYFEQSGNLQGIIMFFSTGGGSGSGIASNLIQYFREKDLTKIVHCNPVFSQGVIHNYLEIYNTVLILHQMIETVDIVTVFDNVALQYICNNNNLDSNYQNFNKIIAESLIQTTASYRFPGFINGGMKKLALNLIPFPRLHFVQPSYMIINKSDWSITNILNKQYKLCSFQDYLPNEYISYQIQLRGNCFEMPESQSLLIRKDDLYQFLGHDTHYRTHTSVQHIDHENIVGFLGNSNGFKKYLQSYQEDFIKMFRKKAYVYAYENIGMDQMEFTEAEYNINDLISEYGPFIHSCCHSEYDNYEEEEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUR8</accession>
    <name>A0A8S1NUR8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Piezo non-specific cation channel R-Ras-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0980173</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="DFEHEDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071260">
        <property type="term" value="P:cellular response to mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027272">
        <property type="entry name" value="Piezo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031334">
        <property type="entry name" value="Piezo_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056770">
        <property type="entry name" value="Piezo_THU9_anchor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13167">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13167:SF25">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12166">
        <property type="entry name" value="Piezo_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24874">
        <property type="entry name" value="Piezo_THU9_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo THU9 and anchor" evidence="3">
        <location>
            <begin position="26"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo non-specific cation channel cap" evidence="2">
        <location>
            <begin position="164"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8095440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="52694" checksum="D5C6C37E7B073AAC" modified="2022-10-12" version="1">MSSGSEKLDQVHXXXXXXXXXXXXXXNSMAFKIYRMIPFLYELRVIIDWTFTTTALDLIQWFRLEDIFANLYICQAYQDSRIENHKLGDPRSSSDKCLNGCLYSMFIVFIIILPILIFSTLNPAVEINNITNGKFSIKAAFFGQDDKLFFTLFQVNDLELLDIPNEQFKKIQKIYGEIETSWQERMQKVRIKRFSDLEWNLPENYRDQIRNMIKSQEYQVEIQCEWVFQKSDSQGGAKSFTGQSIVQAPDTFVNNFGKILAPRAPDAVFDIKVLVPKFLYVGESAETQALRLEPVNIREEISYYQDIRLIHKKTSDFVFFELVPNDDSTDLKQIGASSQNRVEFFVLNELAFGSLIGALSTNMSIISIYATFVLTIGRFLRFAYDKISTRVMFEEMPETYELFDLCQSIYIARVDGDFFKEEIFYELLIRIYRSPEILFKMTGASIYDRQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NVN9</accession>
    <name>A0A8S1NVN9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0990113</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NVN9"/>
    <dbReference type="OMA" id="QEFKIYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="225"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="500"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="479"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8095710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="565" mass="66220" checksum="461BCDD98D0C875D" modified="2022-10-12" version="1">MIQMPESQLGSVRMRPSQIQIEDDQQVSTRRRNFLSPTFSQNLPLFRELQSRQVQNREQDFSNSIITLTDDKVISCHAQMIKSLMFNLFLQAFFVNYFTYMHWKYSHRYVLLQLWIQDLITIFILIYYNYRKGDDCIWQLIETMFLFFFKIFIVFYYEVQEFKIYFITIMMLAVSSLLLIMKLVQKIKAPTKTSAALILQFLKSMCCLQLLLITLKWESKISWSWIQIFLLLWVFLVVCALLLFISFVSCIETLVNIIKKKQQCHYLAGNLWIFIMLIGFTLFPFLFILRTAEFYEGNQLISLAETAKYIVVITLFSLFLLIFTLCFYKQIKAYLKDVQGISDQNDIDQQQTVQSPNSRQLNSPHQTKKLEFVKLGIPLYLIKLSCTYFAVLDKASFEFKKRQKSHQDLESGRSNLQGSTMRETWKKILQKPVASNSMIINKKKDELDLDIQKPAITDNVKVKREEIPVTVSKQEQEEEPARSIKSIKSDGESSENQEKCLVCYENTPNIVFVPCRHGGICQQCAEDVIQKSNECYLCRKTISQILKVQKNVNKQLEVQEASMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NVU4</accession>
    <name>A0A8S1NVU4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0930189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NVU4"/>
    <dbReference type="OMA" id="IPTSNCF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="223" mass="26309" checksum="385A347722B9022A" modified="2022-10-12" version="1">MHNRQHLSIKNLDGLKLDSTQVRRKSCVCEQCGTTALKEDRLPNQLCEIAYHKQQRLADFFLSKLEGARKYMRNDHKKKNFRIFAKNPLFILRSQPDLEESMHLPTSPTKTQTPEKERLKTEQPQIAPYKSYKYIPTSNCFLQNMKQQVQRSNKTINRLLTDRKVVQSFQSSTSDRLPVTKSPQKNKRSFIKQLKPLPKQKLLQTKFDCMIRQFFNSSKAKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXE9</accession>
    <name>A0A8S1NXE9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0960137</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NXE9"/>
    <dbReference type="OMA" id="QRAHVVN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="75"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8094656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="428" mass="50821" checksum="977735860BE88100" modified="2022-10-12" version="1">MDTIKFQNFRQQLPILIQTCQEISEVFGFTLSEALILFHYFKWNDYALNEVLASQEDYDQILIKSGAKSATQYNLEKTCPICYCNQSEISLSCGHSACKSCFELYLREKLKMSSFINCFFLGCTLKIPYSFFEEHKDYQQSYLLRLSKDYRTTVKCCNNQECEFYVQSYVDNIVECLCGTVFCVKCEQGDHRLLNCQQCVEWVKLYEGSNNILDFINHGKFCPKCNQFIEKDLGCNHMTCRKPLGCGFEFCWICMDTWSSHSKETGGYYKCNKYNEQQIEEKNKLKADKSNEKRLIFYAIRYAGHEKGQQIALKKKRRFLDLVSKIQLVDSYKILLRDSYNYIIQARISLKWTYVYAFFQKKQMPIFEFGQADFERYCEILHDLLENEIYKKLKSKQPIEQYHQKLLNNYNLAKQFQTRFLSEFKNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZJ7</accession>
    <name>A0A8S1NZJ7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0920072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZJ7"/>
    <dbReference type="OMA" id="GEANAVW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8093004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="45620" checksum="FE80CEA401D74792" modified="2022-10-12" version="1">MGSNCATCQNCQKKEEILISEVQQQSNPQMNQYSQQPNEEEEEDHLEEKNQDGQNQIVQQYLNLDQPKKNDTNVYAEPTRAQQVDTPQVQVIMTNESNADSKKRKNEMMLRNQEDLNDVQLLKSENTVREKRPRHQFKSGAYYEGEWVGQNRDGYGIQIWSDGAKYEGEWKNNRANGKGKFWHIDGDYFEGDWKDDKAYGKGLYKHTNGAQYEGDWMDDLQHGFGVETWADQARFEGIYQHGKKEGFGKYYWGDGSIYIGNWSENKLCGFGVYTWPDGRRYEGQWANNQMNGRGIYYWKDGRCYEGQYVNDKKHGYGIYTWPDGRKYEGYWSNGKQQGKGRYILPNGKSQLGLWENGKRIKWLDQSEQSIDLKPKDWNSYVQPSLGETQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P722</accession>
    <name>A0A8S1P722_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1080073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P722"/>
    <dbReference type="OMA" id="HAINENQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="469"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8098912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="842" mass="100580" checksum="C052CDAF434254D2" modified="2022-10-12" version="1">MKIHPFKHKEDTETLHTWSSQRQFTEMGSQVGLIQTHSGFLQPLNNSNRKKRAKTVKNFIQFSSTATISPTHSQRTDKKLQFQKLIRKIINAQHFIDELRNYAEQLRSQNLRYAYKRRNWLPLFPDDIPYILWGLIINLCTDFAAILFPLQLAFDFEGVGSQLTLIIQIIFWLDLIMNFFICHVNKQLDLIYNFKDIAYHYLSSWFIFDLISVLPDFNITGLKLLKLIRLIRFFLFERRVAYNQSLDLLKQQKAIKDELVVRNEYNLDLRLKKLIKIFMEMIVLNHLFACLWLWICRFNEETNWLSYYNLNEKDQLTQLIQAFFWAYQTITVIGYGDIEAHNSDEYLLVIIWMLIGVGYYSFTIGNITFILIQSNPNQEFDDQLFNLEDISVNMPEWMQEDLFRFTKYNIQYNPFWADDAKRILFELPHQLILYMMAAVHKEIFRTIPFMSNDINFSAAILPNCSLFCYQQYETIYHIGQSANDFYFLIKGDVRLCDAHGESIIRVMEGTCFGEIESIEYTQRCWSALALQESIVLMCSAHFFSQYLQNESPQFFELQQMYKRRKIILFQQAKLKRQKLNKIRRGHAINENQQLNRKNTIAQQLDNMKQLFDNRPYQSVQQDLMLQIVGQKQARFKIIRERFVNAISKVKYYVQKSQKLKNISVDDPDYRIIQNLINNRVTNSAWSKQKSKKFGQKLLSNFIKACDDEEMIKSFILKSKQESIDRIMRKKLVHIIRNIAKQQKKEKVDKNKIDLFYGKYHQQIYEEIKYEKLEERKKQKQKLESIQQEKRIRTQIQVLNSNFKQLQKLHTSISMAIFEINQHEFDIDVLISELKQQLLLVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PAT3</accession>
    <name>A0A8S1PAT3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Single-stranded DNA binding protein Ssb-like OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1100153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PAT3"/>
    <dbReference type="OMA" id="IHGYNTY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048970">
        <property type="entry name" value="OB_Ssb-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31472">
        <property type="entry name" value="OS05G0244600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31472:SF5">
        <property type="entry name" value="OS05G0244600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21473">
        <property type="entry name" value="OB_Ssb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Single-stranded DNA binding protein Ssb-like OB fold" evidence="2">
        <location>
            <begin position="24"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8099813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="200" mass="23357" checksum="A641DE1EAE8B6706" modified="2022-10-12" version="1">MASQNKAPQTRQLTKPKFDKLSEVKPGIHGYNTYVKIEEIKKEQIKRYDGSTLVVAEGLAGDDTGVIRFRVVGEYANQLEKGKCYAWRNGLSEVVQERHRLSLDQFGRITPEKDDLVAVNQNGKKYSDIEYVRKDARPNTNRRDNRDNRDNRDNRDNRDNRDNRDNRDNRDNRDNRENRGQGQRRPQGNRGNRGGNQKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PEM3</accession>
    <name>A0A8S1PEM3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1140033</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8100948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PEM3"/>
    <dbReference type="OMA" id="QFIYQSP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07852">
        <property type="entry name" value="STKc_MAPK15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000238">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000166">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="25"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="375"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="383"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8100948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="554" mass="63668" checksum="A21844F6F30B72F1" modified="2022-10-12" version="1">MQPQSKSSQKEDEHCQVEDHIQRKFELLEFKGKGAYGVVWKAVDRKTKQIVALKKIFDAFHNATDSQRTFREVVFLEQLVNHENIIKLTCVIKAENNKDLYMVFDFMETDLHKVIRANILEPIHKKYIIYQILKGLKYLHSGELIHRDLKPSNLLINSECKVKVADFGLARSVAKPDDNSNPILTEYVATRWYRAPEILLGSQNYSKAVDMWSLGCILGEMIIGKAIFPGTSTLNQIERIIELIGRPKQDELDAIKAPLADQVISNISTQKRKSITQLFASGEDEAIDFIRQTLIYNPYKRMTVEQALNHPYVKEFKGTEDEISRDSAIETFMDDNHKFTVKEYRDALYSHITQKKKMEHQILIRNLKNVPVNISAEKSTSPTKKDSFLKKSKDSEPSNTDISDNKQYESQNVNRPFHHKTPSYTQQLRKKTTSQEHAYNNEPTNQFIYQSPNMMMQKKQSQSIFPNVDSGSPLKQIPRKQSMQNIQNSVSTVLQNTLSSNLQSQSALQVSQHQLSRKGSQGSIQKSSSYKMSATNYNSTNYNSAFQQLSKIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PEN2</accession>
    <name>A0A8S1PEN2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1140038</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8100958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PEN2"/>
    <dbReference type="OMA" id="YADQSIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8100958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="66" mass="7431" checksum="6CE365E8A97B8BD0" modified="2022-10-12" version="1">MSARLLTAAFRGINIQTYPASIYTLSTQNEQQQLVKVIVPKNLFEMPVVHYADQSIAILDKKPTPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PES6</accession>
    <name>A0A8S1PES6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dynamin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1150058</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PES6"/>
    <dbReference type="OMA" id="KFMESYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051646">
        <property type="term" value="P:mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027094">
        <property type="entry name" value="Mitofusin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465:SF0">
        <property type="entry name" value="SARCALUMENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="612"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Dynamin N-terminal" evidence="7">
        <location>
            <begin position="73"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8101331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="688" mass="80697" checksum="63C957ED9791D654" modified="2022-10-12" version="1">MNFIKNLFVKKTSQQNEPLFFDPMNEKIEQFYRQLNIICDGITLTQDPKDDVQIIRQNIQELKTYIDEAKVYIIFIGTTSAGKSTFINSLLGQQILPSRNQECTQNIIFIKNNEKILINEKQINSLDQAQQILFEMQKNNKFEIVNIQVPSLFHQQFPSDLRSRIVFVDTPGIKINEQQLLKSFFEQIDDKIGNQHRINVWISNYTTFDNDKEFQEQILKIYNTSVSSQISQRQNKQASYLKSVIIEEFQGIQRQPNLFASSQEVIESNESFESYCKPSLTTLFFILNKYDERKVSEDSNVDITIKKISQMIGSDKNIFKISALRAMRYRILNYGSKQAIDKFMESYFLDYRDVELFVNQNDCRKYCNEKIKNNPNLLQNQDYCKFQNQLESSIKMQISETFFGEKFVYIVNLMVYLEMVFYPQQIKLEQNLFETLRDFIDIFIENQIKNYNDCTSKYKQVAKDQIQKELDRYKCQLASEEPQNLIISACKSALQIMIEYEKKILQLNEDIKNGLIMKLQSIFPQKQNNQLFSSFQLVGQKDLSIVEGCKQLFELINDNNVAEIQKNSFQQYAENLQKTMGVRINPIQSQVGIISTGISIAAFLGRRFTIQTLLCSPIFAAIGLGLMTFDLTFIFGKQWYSQNIIEIQLNQWESILIQQIEEQESNYCRINKQTFKSIKKLLQRMMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHZ0</accession>
    <name>A0A8S1PHZ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pectin acetylesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1180090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHZ0"/>
    <dbReference type="OMA" id="WVEYFRD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562">
        <property type="entry name" value="NOTUM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562:SF67">
        <property type="entry name" value="PECTIN ACETYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25617" checksum="AD3516FFC98FF5F6" modified="2022-10-12" version="1">MSKGGTVIVSGCSAGGLAAYYWVEYFRDILPLNVQVLGVPDSGIFIDMKSIDGTELPKLSLIEMQKLVNQEASNPNVECVQSNPNELWKCFYAQYLLRYVNVPIFIVNSLYDTASIKDLLQISCVARNSLSGCSQKERKYIEELHSSIQTVISGRRSIFRDTGSFAPACLEHCFLQTTYYQSTSWQVPGKSGFTIQKSLRQWLSQFNVGNLDNHIDSVDWPSNEACSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLW7</accession>
    <name>A0A8S1PLW7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein BCCIP homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1210088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BCP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLW7"/>
    <dbReference type="OMA" id="NFTHILG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025602">
        <property type="entry name" value="BCP1_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261">
        <property type="entry name" value="BRCA2 AND CDKN1A INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261:SF0">
        <property type="entry name" value="BRCA2 AND CDKN1A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13862">
        <property type="entry name" value="BCCIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8103518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="33505" checksum="F2685956C264B6B8" modified="2022-10-12" version="1">MNQGNQDKRKQRDYDDEDSEEEEIEQPISVHNKGDMEEEINLDFVFLDPNQKQFHSIKTFINGYLEGISFKSSELANIICDQVELGTMVGQEDEDNVFGFTTILNIGEIKSNAIGEILHYVDTKSKQFNKQHQQLQHIFQTPKKVGLFINERILNLAPQLVPILHNQLKEDINWLQKEDPSNSLTNLDYLLVITKCFKDNDQQKQTQKKSTSDLNDLIFQKFEDYVFLQKSVVSFRFLSEGSKQTQQVSDFMKTEQEGQICYRLIYLIQLKDYLEQIVNIEKYVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMK5</accession>
    <name>A0A8S1PMK5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1220112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMK5"/>
    <dbReference type="OMA" id="INYHIEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="53193" checksum="55557693A9B1E435" modified="2022-10-12" version="1">MQIANYQIELPISLDFSYQLLYRGFDETKQSYYINIFPKSELDSIMYENISKKIKSYYYIKDAIEDDNNLLISYQNSNTWIKMPIKITNDQFNEYLKQLYDLYSLDILGFYDFNPDFLYINQKIIYTIDYGLRYSLFQIKYQQRYNNNNYKQDQLMKTWMFGSLLYCLTTGTQDPSYLIKLNQQQINYHIEYNCRQNNISEDNIHFLKILLQTDYDYRPAFDQLANLFKLQNSQIIKIQSQKSFNQQKTIKKIDNLNDNTNCQISKSTFTQSHSKFVPNLHHNKVKLAFKQQNCYTSQKDLKQSKINQYQYASQLIKSNISNKHDQIIKNLSTTKSFYPQNQKQNIITLNEQYYDNSKINSNQNIVQNNQSINIDPINLSRSMSLNVKSQEQQQSIIQTNQMFIKAKQQNDFQSKKKPQKNQINSFEQDFFYKEEDDEEEDFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PNN7</accession>
    <name>A0A8S1PNN7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1250166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PNN7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="64" mass="7584" checksum="EDC06A58147760D2" modified="2022-10-12" version="1">MTQQENEVQASISQLQKQYTNLFIFKKHLFQILAVRNQEQSNNFDQSGLRLTTKDLSNQFLQHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PP26</accession>
    <name>A0A8S1PP26_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1250129</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PP26"/>
    <dbReference type="OMA" id="FQVSWKL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051219">
        <property type="entry name" value="Heterochromatin_chromo-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22812">
        <property type="entry name" value="CHROMOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="3">
        <location>
            <begin position="14"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8104905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="144" mass="16846" checksum="38B3911BE81BE16D" modified="2022-10-12" version="1">MSASPMSVSSQEEYMVEAITNKRVKNGRTEYEVKWQGYSENEKTWEPIENLQSVMTYVLDFEQSLKTKPQEVGEGTYDDGDSADEIIQIRKDNDGQNLLFQVSWKQKNNRAPKVSWINQNSLKMHNPEILIDYLLKKIKWPNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRR7</accession>
    <name>A0A8S1PRR7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1270019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRR7"/>
    <dbReference type="OMA" id="NIHQEDH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="423" mass="49692" checksum="6DF441EA20E4669A" modified="2022-10-12" version="1">MSSIQRQPRSRIFESDSSRPSDRPITPSQQQQSTNQKLRHLTYNTQLTVPIVQCHYHPDQFIQNFCKHLDCLLPLCPACVNIHQEDHNNQQYAPTFDHLSTCLAEQYNKIVDICNLLSDDIQNVTELKTIINEHHKTQKNKFNEAKEKLYKSIDNYLQTLENTINTQSQKQIDYLINQINQFHRLSYERWNHLQTRLQKLNTDKCLKTLIKCYKQSKPEDIYSQQHELSLQFTDQIKAQLNQIQINPSSLQIVMDQLHLYIQIVNENQNQTSPQQIRKSQRPNSQQHTVSTSPIDLRIKGYPIQQRYVIPKNKVELQIPQIQQGFLPSQGGINYYNQAILPLNIQQKQLNNIPVLPFRPQISVKQMINQTPQINPLQPIIQTQENKKGEVIDLKSSRIPLRMTEQYQNRIFNENDFSPKLNIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTG8</accession>
    <name>A0A8S1PTG8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1300058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTG8"/>
    <dbReference type="OMA" id="EPPKRIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="224" mass="27065" checksum="164D228A4CFC50FF" modified="2022-10-12" version="1">MILDIDQEIISQRRNKLRILLNKPNHTKNLCQHQHQIIKSVLEPPKRIFSYSRSIRHFQEIMSKTGSVSVNQKAITLYFKQREANNQKLQSDQIKPTNSKFIQTDDLITEEQNSKTDRTLWNMQESPYIRKRKFANHSYQKEKYPKQLQLHKETQEYHLSNSPNTGRSIKQYTFQNEILKQKFDSIIPFYDRSTHFFNKKRLHQIRHDKCEKLDTIFTTITHRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PVN1</accession>
    <name>A0A8S1PVN1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1330025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PVN1"/>
    <dbReference type="OMA" id="TRNQFTQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="191" mass="22802" checksum="8772C80C961E48C6" modified="2022-10-12" version="1">MDQEIRNIFYPCTDFLSNSLVHAEIQEQSFDHNHFQYTNQNHTQEQHSQRMSQNSTQKMVYKEKDLKYKNFPKLIGNNFLKWIKKLINDEKIKVVPKGIDNLMKSRQKCKQPNFTIKDLQELCSDEASQKLFQEYIQDELFLDIFHSNKIADPFSYIPGISNYFACSQEPQKMKSNYLTRKQFTHISEQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PY25</accession>
    <name>A0A8S1PY25_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1360016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PY25"/>
    <dbReference type="OMA" id="WNDDIEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="70" mass="8246" checksum="4B164A724BC8BDF8" modified="2022-10-12" version="1">MNSNCNKDELITIQSDNKEICEEVQTVKKTEIYQQQINFIVIETSNDELSYRNLLKFKCTLANRIKKTMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZF1</accession>
    <name>A0A8S1PZF1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1380053</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZF1"/>
    <dbReference type="OMA" id="QYNDECR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47026:SF2">
        <property type="entry name" value="FLAGELLAR ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47026">
        <property type="entry name" value="PIGMENTOSA GTPASE REGULATOR-LIKE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="259"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8108777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="283" mass="33931" checksum="9F8ACC05211880B3" modified="2022-10-12" version="1">MNNSRSSARKGTNNLPVQTGDIEEYIQILYEHQKSCEKAGKYIEADQAKKRLVELKKELDQKNKYEVKDRHTNEKQEIEKAHLDEFNQFNEFWDQKMAEFDQEAQRVKEQVLQRHDEELKQFTEELENSIPVKPKDSAELLSLRKTEESLARQENYQEAHLTQQRILAMERDEYEKWNQSRMIKIKNLISQLKLKQTNELGALQQRIISGQEEQRKIRSQELEKLLQKYQNVRKELTSQQNQEITRLDKTMKNQSIMQQSRMNSSKMMNSQMRKGDEENFYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q0Z4</accession>
    <name>A0A8S1Q0Z4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1400002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q0Z4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="50" mass="6142" checksum="607BDB9CFAFC0A60" modified="2022-10-12" version="1">MRKGQDLCDNYWEYQIYLKNNLRKQNVIQEGLYKDGKKLVHQILFRIITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4R2</accession>
    <name>A0A8S1Q4R2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">40S ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1390017</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1430098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8108965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000147">
        <property type="protein sequence ID" value="CAD8110263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q4R2"/>
    <dbReference type="OMA" id="GESDACM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1230.20:FF:000011">
        <property type="entry name" value="40S ribosomal protein S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001931">
        <property type="entry name" value="Ribosomal_eS21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10442">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01249">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002148">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR002148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8108965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8110263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="92" mass="10319" checksum="BF158C7D066A6914" modified="2022-10-12" version="1">MAHRIQENAGMINDEKQVVDIYVPRKCQYTNMILNSSDYSSVQINVGQVDENGVYNKKNSTVILAGYLRQKGQSASALEAILRQRGVLPFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4U1</accession>
    <name>A0A8S1Q4U1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1430085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000147">
        <property type="protein sequence ID" value="CAD8110237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q4U1"/>
    <dbReference type="OMA" id="RQMQGEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="481" mass="56609" checksum="DFE70891D2A1C746" modified="2022-10-12" version="1">MQSDFQEEIVKSEYVQAEDQSFKFVNMPSYNPEQLVEDRLRESIRQSVAQRPINHQDDPTSVKFHRHQSYNQLQAWMEQVGIDMQQHRQIIKDVFRILEKRIAISNRSIQNLIHFIKTFVQHEKQNVEYLRTKNNDINKLFQDGQTLYYPKLDETIKEICNYDQGNAIKILNLLDGIDKFIKEKLDFNIEMFESGISIHRDQYRKAFSNFASSSSKLTKYRRKHQGLYQRQMQGENKGKDLYHTERKLINSFLEMAKLQKNMGQAVTRLFDEVKKQEVHRYQIVITSLRMYLDKHVQLSQSKQQQISAQEELLYGLINQLEPEQVVQSFEVLNFLSQDILRIIQKMLGKQPLTLPQLQDFLCNYPDAYIKLGSVQENHSLIKLRIKNAQIDCGNVLKTWKPCQIIATIDNNILIYLQDTNEFKGKFLCDSSQIVQKTRVPLTAEIKYTKPGFIFDSTKTALLQLEAQDFDSLHGLLFKQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5B5</accession>
    <name>A0A8S1Q5B5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1440070</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5B5"/>
    <dbReference type="OMA" id="DIDIQCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46915:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46915">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="4">
        <location>
            <begin position="158"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8110463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="461" mass="55445" checksum="85C63BF4D7AB9F6D" modified="2022-10-12" version="1">MAEQSSIILHLHSLKDSIKSAFKVFEDIDIQCQVEEIKQNHIKLVEENKQQQEPKYITLTDQKRVLNNFDNIICQLQLVTPFYSDQKYLTFESSDKNKRQTQLKNQQMWDQIKQEQKERFAKYQDETILDKIFQLDTKLKEDYEYSLQYMSGNQKKTIQIKFHDIQKLNPPNYLNDGIINFYLKFIEFELIEQSLRSKTYIFNTYFVQKLCFFEKLQMIGQNDHLKLNELFKLSYEQIKKWIKEDLTEKEYLLFPINLPEHWSLLIVHKKTKSFADSVIIYLDSFGIMDQKLITIIKMYLHKINCDVNSIEINYNDSPIKLIPAYQLLVPRQVNYVDCGAFLLEYAESFLSNPNYLLQDVESQDGIYKLKLFPRSLICNKRLLMKQLLIDLLEFDKDIAISQYQQKRQAIIDQCKNDEDEYDQIDQVIFKEFINQKNQMNIEQRQLLLDFYMNPQQNYYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QB78</accession>
    <name>A0A8S1QB78_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1500009</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QB78"/>
    <dbReference type="OMA" id="EQLNNHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07852">
        <property type="entry name" value="STKc_MAPK15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000238">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000166">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="25"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="367"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="367"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="384"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="413"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8112051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="524" mass="60212" checksum="883B7CAFC3843012" modified="2022-10-12" version="1">MQPQAKQSAKEDEQCQVEDHILKKFELLEFKGKGAYGVVWKAVDRKTKQIVALKKIFDAFHNATDSQRTFREVIFLEQLNNHENIIKLTSVIKAENNKDLYMVFDYMETDLHKVIRANILEPIHKKYIVYQVLKGLKYLHTGEVIHRDLKPSNLLINSECKVKVADFGLARSVAKPDDNTNPILTEYVATRWYRAPEILLGSQYYSKAVDMWSLGCIVGEMIVGKAIFPGTSTLNQIERIIELCGRPKPEDLEAIQAPLAEQVLQSLNTQKKKSFAQYFSAAPEDAIDFLRKTLVYNPTKRMTVEQALEHRYIKEFKNTEEESKRDSPLETLMNDNHKYSIKEYQNALYNRIVQKKRIEHKSLIIRNPNNGSVNLSVDKSTSPTKKESSIIKKDPETSNIDNEFQHPGRALHQKSQSYSQGQYLQRKTTSQEDIQFQVSTHQSPTYKKQQQMFPMVDSNSPINKGSRKSSLDKKLQSTLTAAMQATLSQQQFSRKGSQGSISKTNVFNKSTINTQYNSLFQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QHX3</accession>
    <name>A0A8S1QHX3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cyclin-dependent kinase 2 homolog</fullName>
            <ecNumber evidence="4">2.7.11.22</ecNumber>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Cell division control protein 2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">cdc2-related kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PPRIM_AZ9-3.1.T1650072</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">May form a complex composed of at least the catalytic subunit CRK2 and a cyclin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="4"/>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QHX3"/>
    <dbReference type="OMA" id="HCHENRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000396">
        <property type="entry name" value="Cdc2-related kinase 2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000706">
        <property type="entry name" value="Cyclin-dependent kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF334">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 22 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9 22 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 23" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="13"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="CAD8115618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="318" mass="36732" checksum="C8E6237948680BB7" modified="2022-10-12" version="1">MLPEFGESDAERYEKLEKIGSGTYGVVYKALDKLNGQIVAVKKMTQELEQEGVPSTAIREISLLRELNNPHIVQLKDVVIRNKKLQLVFEYMERDLKALLDSSPKDQSLDKITIKKIIHQILKGIQACHQRRILHRDLKPQNILIDKQGNTKIADFGLARPFQVPIRPYTHEVVTLWYRAPEVLLGAVEYSTPVDIWSVGCIFYELITKKALFTGDSEIDQLFRIFRILGTPNENTWPGVTNLKDYKTTFPNWSPQGFKQLLNRDVDQLAIDLLTRMLKLDPTQRISAKQALNHQYFQEFQVKPIPKKSDYQSLIKFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQX7</accession>
    <name>A0A8S1QQX7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T2270004</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000236">
        <property type="protein sequence ID" value="CAD8118159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQX7"/>
    <dbReference type="OMA" id="EGHTHHV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="66"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="108"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="150"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="192"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="234"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="276"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="318"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="327"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="360"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="402"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="444"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="486"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="528"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="570"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="612"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8118159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="743" mass="81339" checksum="3A5538187AEA7AF0" modified="2022-10-12" version="1">MKVDLSHSCFANIRLKNTSLVGGNFARCNFNESEFDNVDISGLNLNGAQLFNCKWKNLKIQKLNTLDGHSSSVLSVCFSPDGTLLASGSLDNSIRLWDVKTGQLKCKLEGHIGRVWSVCFSPNGTLLSSGSWDKSIRLWDVETGLQKAKMDGHSSNVLQVCFSPDGTTLASSSCDNSIRVWDNKTGQQTAKLDDHTNSVHSVCFSPDGTTLASGSCDKYICLWDIKTGQLKFKFQGHIGYINSVCFSPDGTALASGSCDNSIRLWNIKTGQQIFQLNGHTNFINQVCFSPDGITLASCSDDQSIRLWDVKIGQQKMKLNGHTNSVKSLCFTPDGNTIASCSDDKTIRLWNAQIGQIKSLKNNYCDKVISVTFSPCGIKLASGSIDNSIRLWDAKTGKQISQLKGHTFSVYSVCFSADGNILASGSGDKSIRLWEVKTGQQISQLNGHTNSVYSVCFSADGNILASGSGDKSIRLWEVKTGQQISQLNGHTNDVNSVKFSPDDNTLASGSRDNSICLWDVKTGQQKQKMDGHSDNVQSVCFSPDGTTLASSSEDKQILLWDVQTGKQKFKLNGHSNSVLSVSFSPDGTILASGSGDNSIRLWDVKIGQQKAKMDGHSDNVQSVCFSPDGTTLASGSVDQTIRLWDLKTLEEMQSLDMQYKDVLNQFKIPLYQNIPLPSSNCKFQIGTTNLNTLLISHQAIFQAQGAFIFRGEFVAQSEIDLRSLFRQKGSYILENQLELNKNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQY2</accession>
    <name>A0A8S1QQY2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2190004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000228">
        <property type="protein sequence ID" value="CAD8117983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQY2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="528" mass="63903" checksum="C4309636677A175C" modified="2022-10-12" version="1">MITIFIIFIFLGFQVKIVFGIIQRLKIQTFFNLLHIFFKLLYFQKEMKKIKLSSSTLILKLQNLFSKTLSKKNHYNKNFRFLGRKEKIQEKLMCLLFRQFYYSIIKKIIHSMKNMQKMNYQYHFYGIYGYEITINVLTQFIRMNKLLLLRGLDLIQKLKSLNYVRKWISMKRWMFKFILFKLIKFKIIYLKSIKKEYHFQLEYDITNQIQNNLKIKQMKWKQNLNVIECLQFQLDIIIYQIMKILNEGLRYFIKMVLLYNGCTIFCTQQKMILIVKFLKYILSLHLSEFQIITNFQQQIISTHNTYKKIIIDDQMYQYYRINKMLNHHCDQSSEIVIPKQIILQQLMRSIQIKKRQIIISTFTQIFFSQKLIQIKGFFESLSSNTLQFFLNLIQQLMIMESLKFLNVNYSVNMINPKMLYYFIDIINEVVLQSVNRSISLIRSKILFKSDTLFMCLRVVHVNNTSEEKQQLVLVYKSLIQLLKQILIKIWKPEALTLFQNNSFDLYNSQGGISLRLLTINNFGLYKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QU69</accession>
    <name>A0A8S1QU69_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2610003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000270">
        <property type="protein sequence ID" value="CAD8118772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="LRVIENW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="16029" checksum="21F2563480077220" modified="2022-10-12" version="1">MKEFSRVKTKFCMXKINKKFFSGGGLYNEQGQRNGEWRELKDGFENNQQVILNGEYKNGNKIGRWDIFFKVEERETYELIGGGLYYEVQGGSIKAGQWIELDDDFEFNQLYTQKGEYKNGNKVGTWVKMDIRKKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QW41</accession>
    <name>A0A8S1QW41_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2550005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000264">
        <property type="protein sequence ID" value="CAD8118670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QW41"/>
    <dbReference type="OMA" id="YQKKTIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="346" mass="40655" checksum="91BD3CA116EA6726" modified="2022-10-12" version="1">MFISRGCGVYDENEQQNGFWVQLKRNQMNQYHHAKSLGQYVHGIKVGTWNIFFGNQFVGKGAYNEEGKKYGDWIKLSNYSQFDKRNLNGNPILHKLVTFVGEYTNGKKSGQWQIKQGNIIIGGGLYDDMGYKNGKWKELSNFKLPFFVIQIGEYNKGFKQGRWEILLNKNQGLEKIGGGIYDTQGMKHGKWISLHDNFNHNCKVLDIGQYDHGIKQGRWDIKTIKYYEHDVLTYYFWPKDTFYIIAGGNYQNGEKNGKWIDLDENYNYHNQILYEGEFYKDLKQGKWDMMKHHNSFINRIGGGQYNQDGLKHLKWIELDKNRQKKLILVEYQNGIKICIESMQTVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JLN9</accession>
    <name>A0A8S1JLN9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JLN9"/>
    <dbReference type="OMA" id="FYVNNDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006818">
        <property type="entry name" value="ASF1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040">
        <property type="entry name" value="ANTI-SILENCING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040:SF0">
        <property type="entry name" value="HISTONE CHAPERONE ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04729">
        <property type="entry name" value="ASF1_hist_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="224" mass="26307" checksum="FA92A3ABC7407E5A" modified="2022-10-12" version="1">MALINITNIVFDQDTALFNSPIQMQITFDVMRQLDEEIEWKLIYIGSPNSDKYDQVLEQFSMPPLQQGTMQFTLMSSGPNFELIPSKDDLFGASAIILSVKYRKQEFFRVGYYVYNTYLEPELIENDPPQVLIDRVYRQINTQAPRVTRINIDWEGQMVQLYVNPQQNNQSFMFQQQPIFNDITNNPDYLQQSIQDKIQQPQQQQQQYSGGLNNNQYTDLQSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMT3</accession>
    <name>A0A8S1JMT3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMT3"/>
    <dbReference type="OMA" id="CYIRAIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="187" mass="22021" checksum="DBD3BAE25E847AED" modified="2022-10-12" version="1">MNTQDRCYIRAIVRKTKKTIQNNIKSVLDEDNYGLTDFENNAEEFQSLSEEFSGKCILQNYVICELDTPSHKKNKEVNNQIYLFNDFIQQETVPSEIKIIKKQQNQQNEDNQDSETLSDSSQCYDLNNELHLIAQQGEIDYLEGLKGFKKTVETKQKYQNRSKKIQNKIIKQHQILQIQQKKIIKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRD0</accession>
    <name>A0A8S1JRD0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRD0"/>
    <dbReference type="OMA" id="HSEINME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="154" mass="18467" checksum="DAD3914F43D0329D" modified="2022-10-12" version="1">MSAKKNTNQFEKDSEEEQIQKKVKKNEEQKKDNDEQNSDEFEIDSEQEEDEEIEEDGIQTSSHSSIDLQAYLQQRDQIPDSDDDEEEEEDNDNKQNQKKKTQLKQQKVSKKQQDDDEDEDDEEDDDEDDEEEEDEKPPQKKQKVQQQQKQKKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRD7</accession>
    <name>A0A8S1JRD7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRD7"/>
    <dbReference type="OMA" id="YSKMLIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="352" mass="38800" checksum="2B1D47E56FA326EC" modified="2022-10-12" version="1">MFIIPLFIEIIYACTNTANTGWTNNYQKMLITTANSITNVDITTVCPNIDPAKVPGLTPYQYLSSSSCLGYLGPLGPYGPLSQLGPLSNPFWNPYNFFGQIQLPTNLQQLIQWSQYQYGAPMSKDGPLGYKGPLASTQYYGQQDPGKTLFDTNDFAVQLRAFGLWSALGPLGPLGPLGALGPLGPIGDHGYSVDLNGNYVNGTKVVKTVTIDYDGSSTRTYPLYEFYQSYYAKTILLDTSFLVESDVCQSDDTYQIGSLPFNQIVTFVLTPLLGLDSYSLILQDQFGKVIAQSNVDNYIQTIQLNVKMNTKLTIIVHPIYLSTTIGSYRLFVTGSTQYITQYNISGNHILSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRJ2</accession>
    <name>A0A8S1JRJ2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRJ2"/>
    <dbReference type="OMA" id="RWKTGLK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8042846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="422" mass="50367" checksum="2AE58B93853F05E9" modified="2022-10-12" version="1">MQSIQSNSNSIQPTMGRDRYGQSLSVALPISPVKKLDITNVTLRKYDRYIGNQETIAWEKHLLCEIGDEDLNFIKDFYLEEKSYKDEEFYFRYIDSIRNKYLTGAKEAYTLAKDICKQLVAEDLIKTQRLKKNRKQVTQRIRQTLGTFKQTKNEDILRWKTGLKKLFHEMRDAIQHILDAGGEKEFFRLEELIKQKQHITEEFLKGLPMRNVQEINKYIQSIRLYLSKKTDEFQQHKLEHGDDFHFDEKTIRELSRSSLEDTASCYSDRLTEIRAHSKREKQKEAEKKIEKFYDLNQLKNKWKTTNVDQLKGTIRRVLVKETLSEAEHIRARAFRLEQEKKGIQILKIEQPKKKHLQTAQQRIQERKEKQDKRSVAEQQRRHTLTREEMKTEQLKQEFFTKYNPKSSIGEQFCVAVGYNNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRP1</accession>
    <name>A0A8S1JRP1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0080271</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRP1"/>
    <dbReference type="OMA" id="MEYCSHS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="14"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8044705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="389" mass="45387" checksum="9601926E2451CAF6" modified="2022-10-12" version="1">MSDPLHLSINSKEYIIKNCLGQGSFGKVYKAEIFSQKKIVAIKVQNKISESEKNLIQVFKGKNFKNLVNIYDYKEQNNLIYIVMEYCSHSLYDQIQKKTINPKDIRYVLKQIMNGLSELHNIGLAHRDLKPENILICHLEDRGMMQEVYKLCDFGTSKQYDRLATECVGTPYYLAPEQILKQNYDKSVDIWALGAVMYELFTNVPLFSGSTIQQIYNQILNLDINNKIDSLQALEIKYKNLLKQMLIRNPQDRINLEQIRKIIEERMSQSVEKKTQANLNNLEIKEITQLNLKQPNQIPSIFNQNQKPPLHINHPPKQQSQIQQFNINNQLQKQFSNQNQIKPLQNFNQISQIQNSQQIQNSSQIKFNFQGQQRNSAPDLVSNIQNKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRQ5</accession>
    <name>A0A8S1JRQ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0090268</name>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0090270</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRQ5"/>
    <dbReference type="OMA" id="TSWKDEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01862">
        <property type="entry name" value="Rab7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000086">
        <property type="entry name" value="Ras-related small GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF20">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8045242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8045244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="206" mass="23217" checksum="88BB981346EC79D4" modified="2022-10-12" version="1">MASKKKQLFKIIILGDSGVGKTSLMNQYVNARFTQQYRATVGADFMAKEVMIDDRMVTLQIWDTAGQERFQSLGGAFYRGADCCVLVYDITNPKSFDSLDSWRDEFLMQGQPKDPEHFPFVVLGNKLDKATERKVQESKAQQWCKSHGNIQFFEVSAKDATNIEQAFQDIAKAAASQEKDEEIFFPTTVTLTKQSQKPQAKQGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSI9</accession>
    <name>A0A8S1JSI9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0090177</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSI9"/>
    <dbReference type="OMA" id="EAMTEYP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="295"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="333"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="413"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="516"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="755"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="828"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="760"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8045151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1078" mass="125765" checksum="3823C21F760C8DA0" modified="2022-10-12" version="1">MSDSIDQCNQQSCFQEENFHGPNPIMVSERILLGDGDLYQKSPDSNSFTVRDQSKIQDFSKDLVDAVPMDMHKSYKGLKKSEYQSAYAIEEELETGGKPQFLKLIISKSLQNNFINNLWNRSYLRKLHQLTPFQIQALDDLQFENEVYPEEESIKKEIKICEFIKKFFLQLEVFTPYSKLIFIWDLFQIFTYVLIFFWLPYKISFQLDYIGQLLNQKSKNQMIEIILLTILSFDVVIGLNLAFIYKGIIIKDRKRIIINYFKQYAFVDLVSISTVTIQFFILNDSQEDHDIDNNLTLQILFCVIFYVLRMTKINKILAQIQEFFNLNGSLNDLVGLLKLTMIILFIAHICACVWHGVAFYNDSYSWLDAYNLRDQGNASKYNTAIYWATMTMTTVGYGDITAKNNSELLINNLTMFIASIVFAYSVNSIGIFVSNMYKGTMEYSRSVTLINTFMSKNKIQFELQTRIRSYLEYIWQEEQNMNDDEVSQIICKLSSHLQDELQFQLRGNILRNCKVMVKIFSEKMIKCLLGQMEEQSFSPEERIITINQIDDGCLYVITKGEIELIFEGMNNLNERVKRNSLKYLSQGDFFGELSFFTGEPRKCTAISRGFTKVFKIKRENFLKVLSSFPNDYEKFCEVKHQLKQGDFSSLQIQCYSCQSNSHLIDSCNYLHFCADKEAILKKELYPYNQSRSGLVIRNAKAFKYKWSQYKYIIQRAKDFQTDFYQQPTDNENEENEQPSQMVNDNYTYEDESKIEIDVPSTLNQRSNSRTLSRVSQKPNQIAVEEDDDSKLYTRKVVNPRGTVQTAGFGSASLRESKKFDLTKDYEIEQPISEESSSEEKDQIIIPSVSKSQDRTQKQKITFIRKESSEDIVPRQASLRSRTFTQNKLQQEILKRSATPDKLTSEIEISTNNNNNIKRQAGKKHSTTKTYTKQVQEATEGNQNMTSVMDNQTYLPTIPQIFSQFDKMQIFTHFFPFSNYDIVIKRYARIQKFFGKKRQYPESSKYSFYFMTIKKGWKLKRLGEKLQGLKLVDTIRKPFVKTIQGTKIIKTKNILNTLGNFGQVSPQLKDGQERKDGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWC4</accession>
    <name>A0A8S1JWC4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0100422</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWC4"/>
    <dbReference type="OMA" id="IPYRVEG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026895">
        <property type="entry name" value="EMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011678">
        <property type="entry name" value="EMC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07774">
        <property type="entry name" value="EMC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035791262" description="ER membrane protein complex subunit 1" evidence="11">
        <location>
            <begin position="19"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="856"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 1 C-terminal" evidence="12">
        <location>
            <begin position="666"/>
            <end position="887"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8046527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="891" mass="104150" checksum="52DE9AD09F6667C7" modified="2022-10-12" version="1">MLLLIVSVAGIILNNVLALHQQQAGVHDWNIKLFGELKNIQVQDQTIFFQNTNNQNGIIEKSTGQVKQRNTLTLCDSYNYVRYEPNQQIIQLYNEGYSVLAEEEIIGNIDHCLIGLNGEFILIYGQTIFSTFNKKFTEKFDHQIIHAGYIFDVPVVVLQKQTEICIYQIRESIQQNYCVQSLNNGKIYQFGNTLVYQDDQFVYHLKEKSAKSIDKEFINKVDKFIGLTQTLVISENSKKKIVNLDGQIEYELNQDEYVIQNLETQKYYYSLKQKENELFIVTFDRDTKQIHTDIIEIQTKSSILNAFLIDQNKKQFLIQYQDLQTVLVDNKGIRWTTEQSLISIDAVFYEKYENEEQTHKNSYYESLEKHGTNNPLFLVQNIISRILNEIRDLQEVATHFIDNLGKEKIQTREMEQTYGLKQKVYFLTTYGTLLCYDTQELSLLIKLQINNQDKTFKLVAHHQIDSNLIRHFDNNDAQPTHVLFYYSNEKKRLNTFVLDLQHGMIQQVASQSSNNILWTIPYRVEGGPGQHHNIVILIDEENNIFTYPKHDSLNTKEIILYRNDNGVLRGYKLLNNQLVNIWTINMKDEILIIRSAYHVGEENPRVAIWDDKKVIFKLIDQSNFAVLTSDGDKLKLFIINAKTGKIIYQSVQSEADFNQPINLVFDEHQVFVTYYNKSQMMFEIWTVEIYHAKIEVSFIKMLENYYFTKTPIIINYYKADFEAFFLQQVYGCPLGIKYLGMSRTLKSLTKKNLLIITTSGQLHSLDRNLVSTRRREKADQPILEYSLQSAELPPYQYQLPINFLNMLTYHQTFEIQKFSIESTNLESSALLLVYGSDIFFTRIAPDKTYDMLLDNFNYNALIATTILIIIATKVLSRLVKSSKQVKQFYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0U8</accession>
    <name>A0A8S1K0U8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0U8"/>
    <dbReference type="OMA" id="KFIHNCT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF61">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="236" mass="27727" checksum="EA611B1344AF1617" modified="2022-10-12" version="1">MEKKVVFIRHAESEYNLAQRIAKNSATEVKLEDEDQDVKYSSKYCDAPLTKFGREQCDPTKFEDLRIETVVVSPLKRAVETAVLLFQNHPNKPKFIVEPYLREMFLSGCDFGIRLQETINEYPFINYDRLLSNPIIKQHPNMWSLEFLYNQKSANILKQYIQDTEYGSQACIEKVLEYMKKHPIQVETFEENVKRVEDCKKYLQFEEPFVCVSHSRTIHTLTGKFIHNCTGIEVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3D1</accession>
    <name>A0A8S1K3D1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0130294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3D1"/>
    <dbReference type="OMA" id="QNHHKSD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="236" mass="26902" checksum="2742DFFABE369277" modified="2022-10-12" version="1">MGLLKTKGQSVQKLMGIKLNEEVQPNYSLKNIIPDQKGDILMIINYDLSTTERLDGEFSISLGQLEEDLNGDEISPSRQFDSFKLINQLSIKTKDSVEQKFKQIKRRQNHHKSDIHRPTQLQSIDQKNNQTEKRGATYERLPSDLQIDSQIRQAKRKNIKVSEQSLSRNNLLESNSSSYSSKLGSKQNCVKGILKKQTLDSSFSDNNSRSSSIGSKKSVKFNLSKPDVRMALNQWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7K9</accession>
    <name>A0A8S1K7K9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0180017</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7K9"/>
    <dbReference type="OMA" id="IVIKRYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="295"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="333"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="413"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="516"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="751"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="758"/>
            <end position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8051279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1076" mass="125742" checksum="E5AD4571520FF3F9" modified="2022-10-12" version="1">MSDSIDIFNQQSCFQEDNFQGPNLMMVSERILLGDGDVYQKSPDSNSFTVRDPSKIQDLSKELIDSVPMDMHKSYKGLKKSEYQSAYAIEEELEISGKPQFLKLIISKSLQNNFINNLWNRSYLRKLHQLSPFQVQALDDLQFENEIFQEEDDNKKEINIFEIIKKFFLQLDVFTPYSKLIFIWDLFQIFTYVLIFFWLPYKISFSLDSIGQLLNFKSKNQIIEIILLTILSFDVVVGLNLAFIHKGIIITERKRIIVNYFKQYAFVDLVSISTVTIQFFILNDGQQDHDIDNNLTLQIIFCVIFYVLRMTKINKILAQIQEFFNLNGSLNDLVGLLKLTMIILFIAHICACVWHGVAYYNDSYSWLDAYNLRDQGNASKYNKAIYWATMTMTTVGYGDITAKNNLELLINNLTMFIASIVFAYSVNSIGIFVSNMYKGTMEYSRSVTLINTFMSKNKIQFELQTRIRSYLEYIWQEEQNMNDDEVSQIICKLSSHLQDELQFQLRGNILRNCKVMIKIFSEKMIKCLLGQMEEQSFSPEERIITINQIDDGCLYVITKGEIELIFEGMNNLNERYKRNSLKYLSQGDFFGELSFFTGEPRKCTAISRGFTKVFKIKRENFLKILSSFPNDYEKFCEVKHQLKQGDFSSLQISCYSCQSNSHLIDSCNYLHFCADKEAILKKELYPHYQYRSRLVSRGAKAFKHNWDQYKYIVQRAKDFQTDFYQQPTDNENDEQPSQIANDNYTYEDESKIEIDVPSTINQRSNSRSLSRVSQKPNQVIIEEEDDSKLYTRKVVNPRGTLQTAGFGNASLKESKKFDFAKEYEIEQPISEESSSEEKDQIIIPSVQKTNQDRIQKQKITFIRKESSEDIVPRQASLRSRTFTHNKLQSEILKRSTTPDRLSSEIEISPNYNITKRMTGKKHSTTKTFTKQVQEATEGNQNMTSMMDNQTYLPIIPQIYSQFDKMQIFTHFFPFSNYDIVIKRYQKIQKYFGKKRQYPESSKYSFYFMTIKKGWKLRKLGQKLQGLKLVDTIKKPFGKTMQSGKIIKSKNLLTNLGNTGQVIPYLKDGQVLQDENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7Q8</accession>
    <name>A0A8S1K7Q8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription elongation factor TFIIS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0140183</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7Q8"/>
    <dbReference type="OMA" id="VEGKMCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS central" evidence="6">
        <location>
            <begin position="140"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="5">
        <location>
            <begin position="272"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8049825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="36929" checksum="9450CEDC3091A4A9" modified="2022-10-12" version="1">MDYQELYELRKKLVDWKKNPKETINILKLLEQTPIDQEAVNKSKIYKTLHTLTIIDDKSDPLAATIRTKAQIVQDKLKKLPQNKPNDNDTIEKKREKSEEIKMQKQNSVQNIQSSKSLPDIDLFVNYKVPYPSYVDRVKYLTAISKLFILNIQIFKNQDDLSDSDYQIIKDSVEKMEKAIYYKRQTEHSPKKAYEQDLKILAGFLKKDKNGSLTYRIFSKQFDPLIAAQLKAADWVDDETKQEQSRIIREKMEAEQLGFYKDLSKREMEGVEGKMCKGCGQKKVYLVDEKQTRASDEPTTKFFECYNCGDKFRIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K879</accession>
    <name>A0A8S1K879_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0130360</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K879"/>
    <dbReference type="OMA" id="MQQNQIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000129">
        <property type="entry name" value="Dual specificity protein phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF511">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="18"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="79"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8049246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="310" mass="35587" checksum="39329164A85849D6" modified="2022-10-12" version="1">MLQPRPPFPSGQMDIILGRTENYGALFLSGIEAALNPQLMQQNQIGAVLTVGTELSNLKFDCEQKLIMLHDTAYDPIRRHFEEAIHFIDEQRKTKNVLVHCFVGVSRSATLVIAYLMQIYKYSLQAALTFLISRRPQINPNPGFMQQLQQFDFELNKRRQQRPASTQYVRCYNERDRRESMEVRSTTRQIPNQSPLKRVNQKAMSTKSLFLTPKKSEAQNYFNFTPIVKPNNSLEIKQITKSAQNLDNCVLRQSNYYGNNTKKLAETAINGFKSASSFCKTFSNHFPMSKNQMLAQTQTLLKRTQGSGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9T6</accession>
    <name>A0A8S1K9T6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9T6"/>
    <dbReference type="OMA" id="QVNERSH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37028:SF4">
        <property type="entry name" value="ALMS MOTIF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37028">
        <property type="entry name" value="UNNAMED PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="126"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="480"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="615" mass="74670" checksum="A84815A6658827AD" modified="2022-10-12" version="1">MMIEQQSPQYWDDLILSSSKKESKMQSQTLELTAQKLQQQLEKNKEFNHEYMMRIQRSQSDHREQYISEPHEEQYEDGEQQKTYLNKLMEEFNSTYSPKIKRPCCQPKSYDMPRQQQKKILEYSKEEVWNRLLEDKRNQLEQRENEKRKYIDELVEQHCTFKPIISDIASRRNTDQPVAERLYQDGIQQKKRKEQLKFDHINSEQFSFKPDISQINNVLKGEKQFMKPLYERIDEVMKKKQDELLLKQQVLQQQSDVTYQPKISQRSIQIAEQKQTSKSVVERLMEDAAIKLQKKINQKNEIQEKDECTFNPQINPTTKPVQQINQICQTQYLMADFMREKKEKLIQEFIKNSDITFKPQINKTSELIMESNEERIQENMSDKINRLGVKDYEKNQILKEQIQQAYYQQYTFKPQINHISSIIAQKRSLDDLAYNPERQEKLNRLKEEQESKQSFSYYPQTKKSQQYQHVKSKYDKKLIKQNMEIEKELKERKIQDLKKQMQYQELKECTFKPITKQFQKDEEPLIQKVKGIENFFQNKENLKRKANQQIEREKELFHYELKYDFKNHLEKTKPEPFNITQQNNMNKRQLEEEAIQKEREQCTFHPKINQKYVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAG0</accession>
    <name>A0A8S1KAG0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0190028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KAYIKYG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026306">
        <property type="entry name" value="RSBN1/Dpy-2/CEP530"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354:SF11">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354">
        <property type="entry name" value="ROUND SPERMATID BASIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035896989" evidence="2">
        <location>
            <begin position="19"/>
            <end position="7555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="669"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1098"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1956"/>
            <end position="1978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2373"/>
            <end position="2403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="7481"/>
            <end position="7555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="695"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1956"/>
            <end position="1974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2373"/>
            <end position="2386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7517"/>
            <end position="7528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7538"/>
            <end position="7555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8052129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="7555" mass="867518" checksum="70212C7616A79A59" modified="2022-10-12" version="1">[encoded data, 7555 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KBJ5</accession>
    <name>A0A8S1KBJ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0180415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KBJ5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8051947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="71" mass="8164" checksum="2DC74FC29C26BD46" modified="2022-10-12" version="1">MERIIRSLVNGLQIGQERIQTMLVAGTLVRRKNRVFGRKQVDSQNYIQLNTAFGVYNQLSQKNGKQIELSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KM18</accession>
    <name>A0A8S1KM18_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210268</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KM18"/>
    <dbReference type="OMA" id="RWQQWHR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="583" mass="69049" checksum="5F1258783A5F883E" modified="2022-10-12" version="1">MFLSVSGSGFHMNYRQEVRLPKLQGHKVQAYSMPKHRQLNSPEEKPISPDRFLKYLNNKVQSQAGVYRNPAAVQSSMILIAKQMKFSSDQHTHRQKEKSIKIIEQSNEYKYNLGLGNNHELVQRIMDTRVDWQQTKDNSSMFVNFRWQQWHRGYKYDRLILNNQYKQMVNHFENHPELSNKQYLFRNLCSYCEQNKQNVYDSVPITFVLDFKDDSVDQQFTQFVKFFDKYAPKKIDQMAKKLVEYKKKLKIPVQPMNDKKIQPIPNKIPKTQNGNQYLWLLKPTFLNRGQGIHVVNDLDTIISLICEYQEGHQTIEDDDVKNNKKNNNIKANSFVIQKYIEQPFLINQRKFDIRVWVLVTQDLHCYFFKEGYIRTSCENYTTDDVSNQFIHLTNNAIQKYSDKYGEFEDGNQLSFDDFQNYLNQQGFNIDFQQNNVTKMKQLVWMSMQSVKRKLNLHQRKYCMEIFGYDFIIDQDFKTWLIEINTNPCIEESSGILKMLLPRMLDDAFKLTIDVIFPPKQKQEINESVFPVNKYSNTESLWESIGNLESPKPAVKQTSIVLPNQLSSQIQQFQTIIPIYQGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQV8</accession>
    <name>A0A8S1KQV8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0260139</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQV8"/>
    <dbReference type="OMA" id="TKNVDQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8057628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="400" mass="46696" checksum="84B4F6DAE737ADAE" modified="2022-10-12" version="1">MQKRQSHTKLPSEVIHSNNYYIMPKSLRSSQTSAQSSERKKVVSTKNVDQFEQIHNPEKLLKTNHQNTTYQSNLELQQALHYSQQNNQYLSRYHQQLIQNNVDLQSLYNKSETENKKLQNELKLLQNRISLKESELKQAQDNGVQYMKDIAQLKISIQKLNSTLIKLKEENAQYKEQQNISETQYFLTGQGLSSRKDLNQHNEVNLNGSKYSQNSRQQSQSFQLNSAFTNDNIQSYQNQILQFQKELEEKNQLIIGLQQQIKNLTEFQKQLMTKSPKKLHNKGLKSQMYSSPRQTPINKLQINNESQIFSHRNLLNLIDEQQKPDIQLHRTRQSDEFDVGPSIKSSITNLKSKQQLQQIYKKINQSTIYTAGLYSSLLDYQYLSNSKLLSSSCSKDTLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRT4</accession>
    <name>A0A8S1KRT4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0230152</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRT4"/>
    <dbReference type="OMA" id="RNTITQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="382"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="641"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8055386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="892" mass="104174" checksum="37AC64D112DA71F6" modified="2022-10-12" version="1">MKKISSQIQSLMNRNSFQEPSLSNDIKSSRSNPKTAKTERPQSKTKKNDLLDYFKKERNTITQMVHSVRNTLQGSKSIDILHTQSQKSERQKNGQLNFIDLNVQNEDGNNTKRQILKQKNLSLAIISLENQLFKQSQTTKQSYNQSPQNQIKSPIIKQKDQYLFDMQILKNTKSSQDDYQQNSQIKQNKQSILSDDPSYHRQSIEDMLQNKKHFNHVQTQKQFPHKQCENEKSKQINQIHFKTEICSSQIQSKYQNADETLNYDQDQQLKIILYYKQQKYHYLFKYQTKTTDDLFNYLIQQIACIEKQFTKLGGGTGSTDGDSIQQELNKICQFQTVSKNVPYDYYLTLNELTLDVFKGITLQIQPFYSQTIQTKRVGLKDFTLIKCIGVGGFSRVYMVRKKDNGKFYALKLIDKKFIFENSKEIIVQNERDIMSRMNNQFVTPLHYSFETKYYIAFVLEYCAGGELFYHLRKLKRLNEQDAKIYFAEICLGMAYLHSKNIVYRDIKPENILLDLQGHLLLSDFGLSKPEMTPDDFAYSFCGSPEYMAPEMLMKTGHNYLVDCYCLGALLYELVTGLPPFYSHNTQDIYNSILTEQIQFPNQIQISPLLKDLINSLLQKIPEDRLGHYNGIKEILNHKWFQDVDFDAVLNKKVKPPYKPYPLKYNFDEDEFNKGDAEFRKQFQINLQKEFQNVDDANYLLDNFYYSRDSVYAQEKSRRTNVVNLNQLQNQALIAEPFTINQQTKIQLPQSESNNEQLFQQTLEESKPINGTKGKSDIQDNLKKQEIMTKSSQLQHQTAKLGHTYSKTMQQAQSSLQELKQIKLLIDQSRAQQTSDRITTMPDQNNKKITERFKTEQFGNLASPKTTTASSSLHKMSNFQALFGSEKHKKKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTN3</accession>
    <name>A0A8S1KTN3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270104</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTN3"/>
    <dbReference type="OMA" id="IFIPPQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="342" mass="39887" checksum="A678DCB6A9641B2D" modified="2022-10-12" version="1">MQNNNFRRPYFSVHNISQTSGLTRKARLVIKKNNIKLKNIIVAKFERSLSAPHNMKFFGKIFIPPQMFRSQQQQHSIKESQSPTRLSKIRTGRNTLKLSEHVSNEPSPSVIFNNQKDEDQVKEVQKRKSCDCSQCGKQSVFQKETMNYGVQINQLKREQESAINKFKRSIRKQQTLLFPQYLNSPKNSTSNENIIEEIQILRDDIMIKSFVHRPTLSSLVFEEQVSVNKQSPINSQSRIKLSYFESFLMKQQKIMSYERQKFSRQTGIFESLHKKSNFSNKNTQMHTPMVLTPRSPASSIITGFPSQSPVKKKNQINVYLQPQKSKFFINQNRITTLPEVHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KX50</accession>
    <name>A0A8S1KX50_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">acyl-CoA oxidase</fullName>
            <ecNumber evidence="5">1.3.3.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0280345</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2,3-saturated acyl-CoA + O2 = a (2E)-enoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:38959"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.3.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the acyl-CoA oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.6" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KX50"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003997">
        <property type="term" value="F:acyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000015">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000007">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000015">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000050">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055060">
        <property type="entry name" value="ACOX_C_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029320">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002655">
        <property type="entry name" value="Acyl-CoA_oxidase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF250">
        <property type="entry name" value="PEROXISOMAL ACYL-COENZYME A OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01756">
        <property type="entry name" value="ACOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22924">
        <property type="entry name" value="ACOX_C_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14749">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-coenzyme A oxidase N-terminal" evidence="15">
        <location>
            <begin position="332"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="14">
        <location>
            <begin position="444"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-alpha1" evidence="16">
        <location>
            <begin position="584"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-terminal" evidence="13">
        <location>
            <begin position="786"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF14749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF22924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8059327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="967" mass="112605" checksum="3640396820891D44" modified="2022-10-12" version="1">MELIKKIRISFSYCNSHYLCSICIFINFHSLLLFQVSALKQCLCDQFELHNYIKWEQLRNILQRYLSNLLLYRSLFIKFFDFNYCCLNIYYMQYIIHASVQEYIQDVLLSVYICCLCDSKIITFIPFYVDGYGVTYPMESTNCLYNMRLDRRSYDLQITLCYYLPIWVSFLYNLTIITLVVRRILKHITDFKNKTQVFGLLLYPTILFICWVIVILLQLSCSLAQFNNLTHNQLFSNTYPSFSVTYLVYWMLCVIALLRYLQKLELMDANQNIKMIYLKVILRPKLANNQLKQLIHNNCEYYLINNIKLLSQYNIIEMSSSQDLVKCSFNPDDMGQLLYKENYHKWIKVQELIAKDPISQNNVADYGQSRDKLYEIYCKKAYKLHKLLNYSDEMIPGMVNSQFPETVVTVLHQTMFIPTIKYLGTEKQIEKWIPPSLNYEIVGCYAQTELGHGSDVQSLETTAVYDKNTEEFILNSPTISSTKWWIGDLGLTATHAVTHAQLYINGKHYGVQTFIVQVRDTHTHLPLKGIEVGDVGPKYGYNTKDNGYLRMNNVRIPREQMLMRYSKVSKAGEFIKAQNEKIGYATMMQVRTSIIFNTYVSLAQGLAIGVKYSHFRRQFKDKNGIERPIIDYQTQQDKLIPLVADCYAQGFGCLRIKEILTENLKRINEKNDFSLMGDLHALLCCCKAIYTWNTHFGLDKIRQSLGGHGFLQSSGVVSIQTEFSPSCTYEGENTVLLLQTGRYLLKACNKAQKNQPINENVEYLYNIQQTLQQKATFTRSEQLLCPEIIRKLMRLHAAFYVNKMMLKMMEKAGEYGPKEAWNKHVGVSITEASVAHTYYWTFKTFLDTLIKVQDENIRSVLSNLCCLYGLQRIIDWPIGYFEGGFLNGEQLEIILNAKEYIFTLIKPDLLGLVESFKFNDNALRSDLVDAKPYEKLLQSAKLSSVNNEEYQTKLRSLTKQLREIPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZE6</accession>
    <name>A0A8S1KZE6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0300074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZE6"/>
    <dbReference type="OMA" id="VQYQPIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="283" mass="32490" checksum="25D7C2B9944E2464" modified="2022-10-12" version="1">MAPPTVPLNRPLQAPQYAQSQPALFGQPQTYQPLQTVAPIQYQPYNAPLTYSVACPVQPVVQQVVAPQPILAQSYVAQPVIAQPVQQSVHPSIKGESRIEYIPYQKAITEYEEQEVVQYVPRERKVTDYYAVEYQTEYIPQVYQEKYTEYVPMDRYQERVEYYPVERQVVHQQVVQQPVQVVQQPVQVVQQPVQVVQQPVQVVQQPVQVVQQPVQVYQQPAQVYQQPVQVQSIQTVPVAYAQQYASPIISSRVVPAYQPQQYQPAAQRVQQQVPLRSDLNNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2S1</accession>
    <name>A0A8S1L2S1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0280166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2S1"/>
    <dbReference type="OMA" id="NQLYVIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="327" mass="39422" checksum="B5A9656E65FE416E" modified="2022-10-12" version="1">MINNLRSQDSVQMSMMQQPFSFQNVYHSFDGNDQRIKINQLQIIQHKFIKCLNNLIRHQNNKKKSNIIILPNQNNQLYVIFEDDKILRKIKVQESNDSTFMSFPRNQFPLIQYQPYPQFYANYPQEQFQTFQYQITNPILQSQDRFLVQPKQLQSSQHFRTSDFQFFGSHDDSESMEPQQVEKDSKEHKNTYFKLKSKIKKGKVNDTKNITKNFSKAIISYIIQNKDIGLKIMNCEQFEEFVTILKDKKNQMTNIKQLRELWVETNQEKSQQFNKAFRIFSEYFLKQQSVSYIYNSRIANTGWHLKYRYNLLRALKEPENFKYIKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3Z3</accession>
    <name>A0A8S1L3Z3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0310071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8060965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="31773" checksum="A884C7A4D5480624" modified="2022-10-12" version="1">MIISYAKLFYFQLRQRIXIQSLQVERNRNKINFKQVQDQLQQHQLYNPTTNKQSGRATELSKVIKNVYTIHDLKYQHGQQIKLKPLTLKVLWEGILTLHRVLQILMLYDDIISRPARFALIYAKTILVLALSALFSGNMGPVYGTLISVGIGQVINVILSIITVTMKASPILKFLGILLTIAISAVCWFIVLMLSASMEETQANMWAVQFASTQVIDLIIVEFLVISLKLAIYPWAIKALHDPDSNASKILANLLFIAVAQPQISKFFEVEDKKDNNFEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3Z4</accession>
    <name>A0A8S1L3Z4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0330021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L3Z4"/>
    <dbReference type="OMA" id="QELRCYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8062207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="54" mass="6337" checksum="24B844526A05711A" modified="2022-10-12" version="1">MKDHCNKAQKITNSLTNKIFKRKVAPQLISFSDAQELKSYKKQLAQRIIQDFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L7H7</accession>
    <name>A0A8S1L7H7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0340291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L7H7"/>
    <dbReference type="OMA" id="DHVSMIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="348" mass="40689" checksum="F67C59D23DDC662F" modified="2022-10-12" version="1">MKTRRSSSAQPSYQSEEDYIEKNKHICKSINPKLTFRQIRNKLSTEWNILANKPKKQTRLDDFIISISSSPKQIQQKKQKKISIQKHRKHHQINKNQKSLQNKKFKQTIIPSSPKQVPSIIITDSESLKISLTSQEEKQSILRESPVIISTNSLQIKEVNQQPQKEVNQQHQKKITNTKQEESKQNLIQNQKVQKENKKHKLKLPIQKLKLRRLNKLSKLKQKCKPGIPSEFQPILAQDFINSAKKFDHVSVITISQSKVDQGQVGLDEIKNIVSHTYGIINHEQSNSERLNFQLFFEVEFQPREDGNVPQNTFCTSKQAAFYCKDITLGYIQKHKDIFTQYNEYYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L964</accession>
    <name>A0A8S1L964_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0350285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8064077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L964"/>
    <dbReference type="OMA" id="NVSMSDF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8064077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="118" mass="14059" checksum="C292BD868DD6C24A" modified="2022-10-12" version="1">MKTRNSQRQSQREDLSVTCQENISISEFPLNSSAQTPRKKRGPICKKHKYTTINLKKHKSSKQFICYEDRDLNIPEKYQSILQKHKTDDDRESDSEQIKHAINYLYKDLLQCIEREKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LA25</accession>
    <name>A0A8S1LA25_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Papain family cysteine protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0330296</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LA25"/>
    <dbReference type="OMA" id="IKEEWHT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000332">
        <property type="entry name" value="Cathepsin L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035907149" description="Papain family cysteine protease" evidence="3">
        <location>
            <begin position="21"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="5">
        <location>
            <begin position="40"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="4">
        <location>
            <begin position="125"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8062692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="39420" checksum="A1D8A94AF8855F0C" modified="2022-10-12" version="1">MQKGLLTLLAITLLAGTSLLNTTINNESNSFLEDEVMKVYQNWQKEHGKRYTQFENSHRFGIFKKNYEYIQEHQKRVDAGLETFEIGLNNFADLSVEEFEAKYLKYKSTPREQTKQVYKKQGKQVPVEVDLRKDGVVSDVKNQGSCGSCWAFSAVAALETALRQAGVKNVELSEQELVDCAVKDEFESEGCDGGEMYDGFAYASKYGIAIRSEYPYAGVDQQCAAKKTKTRYQFAGFVDVESLNPQAYVEAAAEHALSIGINASGINFQLYKKGIYNAKCDGSKPALNHGVTNVGYAPDYYLIKNSWGISWGESGYIRFARIQDKQGQCGAQQEVNYPLYTKFENQSAIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LD82</accession>
    <name>A0A8S1LD82_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pinin/SDK/MemA protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0360158</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LD82"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006786">
        <property type="entry name" value="Pinin_SDK_MemA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04696">
        <property type="entry name" value="Pinin_SDK_memA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pinin/SDK/MemA protein" evidence="3">
        <location>
            <begin position="81"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="231"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8064501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="262" mass="31189" checksum="21292E93AFD6080C" modified="2022-10-12" version="1">MNVEDLRNELNELHKKQAESLTKRKTIIKEIKNFTFQKQDYKKIKLNDGKEQKVFQINLVGAKEERAEKQKQLDEQVPTISKEKDRRLAKSLLGNHLLKAKQDLIGEKQKLEKQIEINKRLDDKDKLDLQTLKEDQNKKKSQLVNERKQLERDLAADEYKRQVLLLELQIKIMRDFFITKTYPHILWQPAKTNEGMNPLKEYSSEKFNEMEKDLKEKLLKAYTNFSESQYQKLKEQQVQQEHESSQSESEESQDKQKAKGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LGV7</accession>
    <name>A0A8S1LGV7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0400060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8066997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LGV7"/>
    <dbReference type="OMA" id="ILMFANN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="58723" checksum="D26EF6A003AC4760" modified="2022-10-12" version="1">MYKYSLRFKDSKIEEQYLIHRSTTLQFPTLTYVTGGGFLLLIINFFVHLMGQSYQKAMKNGAIAFYLLFQYILLRKYSKFRKYSNFALLICNIIFTAFEYDNPAPVKTYYDGYILGSNQMLVHNILMFANNLKLGIVANLIHTVSKILIVQLYQGNMDIQQYVYTTVLSLSFIIIQYEIEKQYRNSFFLSLKDSTWEIMTPLFLKKPYFLFSFNKEENAYQVISSYLKEYFESETPLTTFIYQSKVLGNESLQAYIQRLTVKSLENCNNLCLFNQYLNVDYLNKKVAISIIAYQFEKVIYAIIIESEDPIRKQQKMKYLQQIQIYKEFFHLQIMPINKVLATILKENHHPLLRDLRISLIEIYYQQSQVCELKLVTIKKLLLKCVQLFQTPKYSISIVCNDDIKFITMKHALIIFLFEILKKNNGQFLQIRISQVQNTSIQIIGMNHLPQSELFQKSKEMLIERVLESQNCFMFILMKTPHQNFNDYFYIQDSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHN5</accession>
    <name>A0A8S1LHN5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0400225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHN5"/>
    <dbReference type="OMA" id="HFNEDYA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="599" mass="72574" checksum="9F1FB1B9467E4F0D" modified="2022-10-12" version="1">MIQKTEYLADEAKEREYRIQRMEKQLIENQFSKQAYPKKYDYTHSKNNYAVPINQLDYKSFYQSYMPSDQKQSQNLQFPQNKTSSFKLSYDINDKDLTPNKKQQQLQYRDILQKQVEEKQILLQQQKIKHEYTDIFTNNQNNTPNNKINSLSKAEYQNYLQLQIQEKQSSNKIYNTPIKNQQHVEEQNKDKNIKESKEFSIIQKQLKQYQEEKKQKDPKLLLQQKFEESTSIHEKTKEKLFSPEHHNKKKNLVQEDEEEKFRTQQQKSFVSKILIEQIQEKQKKRDEEKKQQETEDKMAEERWKNEVQIMKDEKKREEELKKEQQGKIQQENIRMHQLKLKQQQGRNRSKSNTLQQSKIEEELQEELEMIQQEIEEDQQSIQKNEQLEEIQQQQQQQQQQQQQQQQQQQQQQQLNEFNIKPNKTLSYVDQQNLEIEKIKLQMIQQQQQLERQIHAAKNLAQIGLLEGKNRIEKELILQKNKNNKHFNEDYAIALLSKNQSKYEYELPFLFNQKIQQDQLYRDNLIDKVQLNTQSKLIPISTQHNKNPSTYLVNQQQKQIQARSKSISQHSKQNYHKQLIDFKKSKQNDALSQLLLEYHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LN36</accession>
    <name>A0A8S1LN36_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0430211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LN36"/>
    <dbReference type="OMA" id="SDQINSR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="172" mass="20116" checksum="D2213D8BFF2321AB" modified="2022-10-12" version="1">MIKNNSKSYSNIRKKSTLSILEISKGKQQVVTNKTRAKSITSTIIKKPIPLLKPPEIDLNKIDVNPFTFANRNSHNNSSDQINSRSQNSDVEQQMNVAVIKQNNQDQLQILKRQKQLLEHRLMQETESCQQYQQNYHFLQERLQKLELAKNNYINEMNSFTKCLKLILNKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LS09</accession>
    <name>A0A8S1LS09_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CSC1/OSCA1-like cytosolic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0420074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LS09"/>
    <dbReference type="OMA" id="QSNTYEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF114">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="388"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="560"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="624"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="664"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8068273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="758" mass="88127" checksum="02D8FD180AC1904E" modified="2022-10-12" version="1">MEQPPESIFASIDNCFDHSIVYYFRILKWNFFLMLIFAILHLPQLIFCITGNSIFNQFQFQLETFTVANVDISNETAFLIGVLGDAIGTLLYFSSLLYLRYNAINKDSQFKVSSISPWSVEVCGFPSATIDPEDLRSIIQSNTYEIYLVRNFQGTLSIMKNKAFKERQIRLEKKKLEVFSKRLSQADMYVRQDFIRVLTDELKDITKELSDKFNIFLAPDDLESIKAFVVFKSKVDRDLFYEKYNQDCLIRNGIYLFQNQPQELKLRGLYTIRVTEAPDPNEIKWENMDYQERNNIQIILMHLAVAIILLAPLIVFENMALKNARNNTIKCTEYCLDNDFTRTVYYIIIGFWIFIADILGWIFMELIINKEKQIYLVQEQKVVLIRIIVYLICYTLLVPILISWETIGNIVLVIQSYTSTEKQLIFSDDLDRKWIINHGLIFLWVAIFYTLKLIILGIASGCRCITGGIRDQEELQLIGSLLTDENEEERQRLTGQLKTTSNNNNQSHQTLFLLNLDESVNYNIQSRQNLDIKTGKQFRRDSPLINSNPTFKTGLRYAKLLFSIFLSTTISAGLPLLPLLGAIQIGMQYIYDKNMLLRYHSYNEKQQTKLQEQLKSVGIVTLKFIHFFLILHLIFSILIYGYPYIYTQNGMGISSDWNDASNKLLYKVSTVIPLTILLIILVVALIIDLVFFGINNNWKIQSDQIGQDVSQNLEDHVNILKEQGSAISYNLKHHDEFRDILLSQKQESIRATLNQFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYW0</accession>
    <name>A0A8S1LYW0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0500268</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYW0"/>
    <dbReference type="OMA" id="HYAPYVR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000128">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF511">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="48"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="103"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="187"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8073220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="292" mass="34127" checksum="AB07A2093035300D" modified="2022-10-12" version="1">MINKIGTLNIKIQYNTSLIEYYFQFICLQMFHTYLMPKLGDPMNCIIQGQNGKGGLYLGNIESAGNGKLLGHHDIGAILAVMSTKDYTYDAHIAHKFIRIDDADFVNLSKYFEEAIDFIDINRQQTNVLVHCHAGVSRSATIVIAYLMKTQNMSLEQAFKHVQNQRRIVNPNPGFMRQLKQYESKLQGSNSLRTSTVKQKERQHSQTNLRQSQYIQHPTDNYNSLYQQYQPHRQYQSASDLMRQSQYNYERLNSRPMHYPYMPQHYAPYVRNYSVPYGQNPLNFSTYRQGCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZA1</accession>
    <name>A0A8S1LZA1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0500075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZA1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="49" mass="5691" checksum="43303FC1269A6514" modified="2022-10-12" version="1">MQLMKILRLGISKGLNFELIQFLPYGLLIGKNKNLAFAFWALLLFEFCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0I2</accession>
    <name>A0A8S1M0I2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0480190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0I2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="417" mass="49006" checksum="70E8DB2577B24B7D" modified="2022-10-12" version="1">MKSIKTKLIANTSQIETTINNHSITSRKVPKLPQLNMKYNQVLSSSRKLVVEENGVLAQLNTQKKELIKLIYERQLDEALEITKKLVQQCISYDDLTIFGKSLHLLADMYISNREFNNGLYIFNVLRILADIQNNQTLKIEALIQMGDLCKLQQSYHLSKIYFKKALQYVWYLNDTDNEAIIYDYFGVLYYVQGELRLAQEYHDRAMNFIKESNDSAAKNHSVESIKAYIKRINYQSQVMNNMVLSKLGLIQGNETDIKLTTQLDFATLNNQILSDSEFLVEVTTPNRPQRCVTKNGILQKDIDMFENVKKLHERQLKIKFFEKKIPVMQQTKTILKQTIDEQLETRINSDQNKNSVERFKQHLQKFHTFKNFPEKLDKVNINHLSPNRNLLGFKYSFKPMRHQLLDLFSHIEQYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5Q4</accession>
    <name>A0A8S1M5Q4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0540195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="289"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8075417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="361" mass="44037" checksum="4592EF3826791650" modified="2022-10-12" version="1">MLEVLHVGQYILQFLQIIFVLVNILEYPLYQYIXYLSKPLSHYFALQYPTLPELPQREFPKLIYDSRYEIEDKYYRNQIQNQQQQDLAQSQQEYQKLDRTLNKYNQFKQDSDNMNLTYDNKQKNNQQQEPTISEKYNQITDQDIRFVSYVFQEEIQFRKEFVQINKKLKQLKTNLLQLFFAFTSDNHYKIEKFKFLEVLQSYQDDIKICDLELLFNYLNNWKRSQSITYGQFLQLIIPYVKYEEMPDPGDLKYQQDHPTQEQMEIFCRLIQMKLQMMSTLEYYRQKIDKSLINLVQIFEFIDQDRDGWIKAFEITKLLQQFGYLVTIEEASLVLQDIDERGQININQFVYYYKPKSLAIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M7H4</accession>
    <name>A0A8S1M7H4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0550212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8076057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M7H4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="273" mass="32208" checksum="CA8118A6813B3FA5" modified="2022-10-12" version="1">MKIIDLQINDGKHGFIKQVKIGDKLYALKQFKDKGGDQEYRIHKLLKHPNILNFIYGNEKYIITELMIPYDLHFFVKTAGSMSVNTSNYILKQLVDALKYMHQLRIAHRDVKLENVLVDEQNYQIKLCDFDVSESLDSGQVHNQVGTPCYMAPEINFYGKIDAQNLIECDIYSLGVLYYILLFGKYPFNTTNSSCPLYRLILKNNWNKFWYYSQRNRSEKIPISCLKLIEGMINYDPKKRLTLDEILYRIGQINEIEYINEMKNVHHKLQEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCQ0</accession>
    <name>A0A8S1MCQ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0590113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCQ0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="50"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="95" mass="11177" checksum="16A4B0F562759C1A" modified="2022-10-12" version="1">MYFQQTNSAQRNIPVIQLQTPLNNQQYSSPTNQYYSIPSQTRPFLESPKANNMKEQLDSLYINLRKENQSLKDKLQHISAQVDEAQQKLEQYKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDZ5</accession>
    <name>A0A8S1MDZ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0600062</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDZ5"/>
    <dbReference type="OMA" id="NNQEAIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8078468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="235" mass="27524" checksum="9B983F9FE8993516" modified="2022-10-12" version="1">MDQQVQETQNHYEIIINEVIGKLKKNSDKGQLQMSESEIQTFKDLWQSSLKAIQGGKENRLKIKVSPQIKQNIDNPIEQEPAKRKKVKTNVDTQIIKKEEADVVFTDESGDETSSKEEKAIDQKAMKYLNYQIQKNECLREKTLFDKIRELDQLKNLVKVEEEDDGETEEPRVFDTQVYAQKINTENLVSRIRPNKSQKMSLEYVLIREKNNQEAIYPQAQLLFNFKTNKKKNSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MI06</accession>
    <name>A0A8S1MI06_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0630096</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MI06"/>
    <dbReference type="OMA" id="ISCKFKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8080107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="18000" checksum="328379F21ACDAB85" modified="2022-10-12" version="1">MIGLPKSGKRTLLNQISFKFKNTQIYTGDLITALKYSKFNFLCFHNINNNSIQNHLNPVFNKIDALIFVVDSSNLADIKNAHILLQSQLKQLNEIPFLLIINKYNLAQITIQEIVIQFELIKLRQPWFVKTVGSEISDTETALQWLYDQIYNIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MM99</accession>
    <name>A0A8S1MM99_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0590070</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MM99"/>
    <dbReference type="OMA" id="RIKDYCV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336:SF3">
        <property type="entry name" value="GUANYLATE CYCLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336">
        <property type="entry name" value="OXYGEN SENSOR HISTIDINE KINASE RESPONSE REGULATOR DEVS/DOSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="543"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="8">
        <location>
            <begin position="675"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="363"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="269"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="363"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8077915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1020" mass="117996" checksum="E5173B40E8B905F0" modified="2022-10-12" version="1">MAELDENYNPWDLESDDYRMPGQVIINYNSHHNEPQPVSNYIYTMLKTQTVRLKIRVDPLTLEIITFTEKPKCTPLVKKEIIEIEEEEARKMGSPSSHEAISLQTPKSVRSQQPHKTKAVEKIRARLSKPQILSKNQISQRQLQQFIDGWGFSLLMAFVTIYALFGDDIRILSVNKDGDEYFFILTTICIVCFTIEIVLTCIANPAYLFNFYFWLDLISTATMILDIGWITDRWYGDEGDVQNAATLKALGKASRTARKAARVIRIIRLVRLIKLYKHARLQMEKEKQKKILQELLLKDEHKINSSQQQQQQQQQQQQQQQQQQQQQNKIKSNYLIRISILQQNRQKKPKSEDLIQQLPYINKSQHSRSQSQHDQNSSNNEQQQLKQSNLSQSDQIKESHVGSQLQDLVMRRVITIIIAILISIPILTLDTYSEIINSYDSGIFRISQFRKNLAITDMLIQQYVQFHNHEIYPIQSVLVLQDDLNKIYTEYNYTTNPDWFQHTELFKSQYRFSDQQYFVTTDTDNQLITYSVSDLVEYNKINAILSIFQTVFVCIVLALSAVLFNKDVTELIIEPIETMIQKIELIANNPLEAVNIEEQEDLITQELEKNKDFQKIKQRQIEQTTETYLLQKLIMKIGALLAVGFGEAGSEIIAENIKKGGSVDPMIPGKKVLAIFGFCDIRNFTDATEVLQEDVMVFVNEIAEIVHFTVDSYGGSVNKNIGDAFLLVWKYPDMKYHVDPQTNKLILHNDNNVKQIGDMAVLSFLKIIISVTLSKKLEKYKRHDGLNARIKDYCVRMGFGLHLGWGIEGAIGSSFKIDASYLSPNVNMASRLEAATKQFGTNILISGILKRNLTQSCQNHIRLIDIVTVKGSIEPVELYTVDLSIKNLLNKFKEPRDIFDISRMNQREQKQFRVVNRYKRNLLIKSVENDKIQLAALFEKDEDLIIAREPYTQEFYDTWISGFNYYIRGEWDEAQQIFMKTLTMIPEHKDGPSNTLLEVIHSAGGRAPNDWKGFRELTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MMB1</accession>
    <name>A0A8S1MMB1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0630273</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MMB1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="262"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="130"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8080452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="582" mass="69678" checksum="18AE5D0321718BDD" modified="2022-10-12" version="1">MLNQYNTPPRIPKPSPVPQNQEQYLHVVLHERQIQQQQRYYTPPLIKPPSLQTISTQLNSPISNQPKQQQYSVIQQVNQNDMQNQYHQIMQYYKELDMNMQQQKQENYLKYNEILQKIDNIQQIMVYNINQLNINQRNEYQQLLHELQQQRLENQKRYELLIQNIEQQKDQNHYMNQQQQREILIQQQHQIIEQQKPNLSNQHHNIIKPPQPLYQNHASKPIYQEQKLIEQHQQNQLNYSQIHNRSSKQSSPQVYMSKRNQSQDNNTFGINSGQLNPFIQQSPSQINPPEQYQSPSNKQIINNQMSNQIILQTKSPRQQISPCICMNSPNNISRDNSISHQKKQIFEKPIFEQFNNQPTFNYNPKINNQENPVIQQYQVQQPFNSNESIIIDESNNSSELLQQLKLEYGINSLPQNTSLQPISNQLQLIYCLQCDQYISIQLSDQHLQVCNSQRPQKRQNDNYLDKYIIQMGDNFIIETFEAKYIRICKEIMRIKQMMQLAQLQKWTTYEYLQLRELCQIANIILDRLLQNPDSSSIIKYYQHIQQIFNVVYMAPQIFYKQQAKLLQKCLDRIKELQQLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MME3</accession>
    <name>A0A8S1MME3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'(2'),5'-bisphosphate nucleotidase</fullName>
            <ecNumber evidence="3">3.1.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0620097</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.7" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MME3"/>
    <dbReference type="OMA" id="KEWAFSG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008441">
        <property type="term" value="F:3'(2'),5'-bisphosphate nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000012">
        <property type="entry name" value="Blast:Putative inositol monophosphatase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050725">
        <property type="entry name" value="CysQ/Inositol_MonoPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028:SF5">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8079581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="35922" checksum="E58F2E48C5043668" modified="2022-10-12" version="1">MLKVHEFFSVATQLAYNAAIIINQIRLSTDIGQKWKAIDDPVTIADIKAQTYIIQQLQKHWPQLKIVGEESTVYDQPIDLPDTFNNLYTEDIFNKTPNNLKVRTQYEIEDLCVWVDPLDGTLDFVQGDYDCVTTLIGLSYKKQALMGIISQPFVKIADKQYEFKPKIYFGHHPQRRVFYTYDTKQLIPFELLKPSYDPSNIILCTSKNRITETKQKQIQSLGCPLLQMGGSGKKSLSILEGKGDLFLYIGVKMSKWDISAPEALIKAFGGRFVGLEGQTYDYNHEDKTYDNPYGLLASIHSELVDKFLPKTTGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNK1</accession>
    <name>A0A8S1MNK1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0600238</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNK1"/>
    <dbReference type="OMA" id="YCANCNN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LITAF" evidence="6">
        <location>
            <begin position="21"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8078763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="108" mass="12156" checksum="A772044B927EF289" modified="2022-10-12" version="1">MNHLGVGQQQKKTVICNNTEVQLVIPQQPQNIQFTRQPHVVYCANCNNQIQTNVQLKIGKGQLLMALIMLPKLLCACVIPLLMDDCRDAVHLCPVCQAEVGRKNFIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MS14</accession>
    <name>A0A8S1MS14_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PiggyBac transposable element-derived protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0690176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MS14"/>
    <dbReference type="OMA" id="KNQQWIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029526">
        <property type="entry name" value="PGBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13843">
        <property type="entry name" value="DDE_Tnp_1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PiggyBac transposable element-derived protein" evidence="2">
        <location>
            <begin position="392"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="892"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="907"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="942"/>
            <end position="952"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="982" mass="113902" checksum="65E794B99542D6A8" modified="2022-10-12" version="1">MSEITSNQPQLESQLLYELVNQIPQPIIQNQSSQHVNIDPLPKHDQIQQTNNTVDHIIQLLIQEDNQDPQQRNQLQHQSPQPIQNQEPLQQDDQPIHPRQDVNSLVILAALESNDEDKKSQEADCKLLYKKKRQTSIIIDDDIQDDDETTPKKTAKNEKNNIVSPQSNDDPLANSQNDSEKQEVVLPPPSQRSSEELQPDIQKPFENKTSEDKSSLLEQMLKPEPALKKPQKEFFSFTQPIPTTVSSSKKNKNPIVIDSDDELQPPPNISILKQKIEDTTPSSFFRKKPIENQTPIEVGENENSQVEKQQKITQLLQQPIPPPQNKPLNQQQRKHQALLEEIVQEDDGIVQDIENQDWTLFTQLNNMIAPKFSGQFRMLEYKDNPQDMIRLLFGETRFKELMKTCQTSEPEFWLYLGTKLIMGYMRLPDINEYFYGEDWIAGGGIKSIITQQQFDDIDQRVEIIQPKLQTESQLAVKLEFNAQSFFRQEFLPQFTQELNDRFKKLILPGQELFLISNFYTLIYSTKIQWYQLIDKESGIILQQFFWATPINKTLDLNSHRDLQKRLRMMFEPYHLGRHIVYSQGLLNAESVLQLYQQKIFVCTDLMQHSLLPHPPTGDQQVFYSSKNKEPIYLCYKNQQWITCVSLQSLQQRNQYLQELSQMQFTEEKIAIQPSEPENTSINSNSKIIFIFVIESILHNIRILKKQEIKTFKTELALQLINKLIDYKNPKPLLRKDGSLQVDKSCQTDQTVGIVNDQLYHCPIHNGNARCQVCLSKSILSKTTASCLGCNKVLGTNIFLCIYPCFRLFHLNPKLYLKEGMYYQIVCGYDQYDQDQEDEEVDQQFLDANLRFKPDTSELDSIYAKSYQDFKNMIPINYEDNYHKKTTIVVIQREKGGNRRGGGRAKASRQQQQSIQNQGAQAEQGNQGDTQDAPKKVRKQNKKSVNTPQQQGQAEKETDQASSAMFDFFSQVRKQAYKKQSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MUF1</accession>
    <name>A0A8S1MUF1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0640110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MUF1"/>
    <dbReference type="OMA" id="ICETPQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8080683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="293" mass="34276" checksum="90A0A2D0D618452C" modified="2022-10-12" version="1">MSEVNSEEEIDEQNPTKITDTSPIKKGDFMTYSQLQKKINSVIPDEETVSYNTSAVQNSQLTYSMSQFERSNLVKIEEVNESQHSLFSQLQQSHFISDNKESLKKDQEIEILKQQNAKQQKYIEELQIALQKLQVCQKEEINQLRCQLDEKDKMIVRLSNFEAYTQEQQKIIDQKDKQLKDLKEQYDIRTYNLQQIVDDQTESIKTLHKQISQSNNNSQIVNSPNLVEKIRLKQQNLKSQFEELSKLYDEITCETPQTPTSSKKQIITRTPSRSIHLIQSPQQYNTEPKPSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MVA1</accession>
    <name>A0A8S1MVA1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0700002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CSLASEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="767"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="829"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="877"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="909"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="965"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1100"/>
            <end position="1122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1229" mass="143308" checksum="0B3378F77DA007AC" modified="2022-10-12" version="1">MLPSNQIISNYKVFNPQNYNYLTYIKEFQINYKNSLNEKLINFSNSSCEINIFTYFPKNQTLIQSNNNQTIFYNQSKNCFDLGSLSIIFNPFKDDDIYFQLQITCSTLLEQNILQYIIKGRTLKCQLGEFYFNEGCQQCESDKGFYSVIYNTSKCSIFDKTKFKNVTQNQIELLEGFWRPNHLSDYTELCFKNQNFCLGGWQIGNELCQRGHIGALCEECDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTLSEINCTICQNGLINVNFIDTHSFLLIQSLKLKQNSCGKSSCVNVIKLSTLQRLLYNLVSQVNEKQFDLKINDYLCKYNQAYEGTCLQIKNIKTLIQQSIFEHNYANASGGSIKVIGNEIFYLVQSLIYNNTAKYGGGLEIEDQMSQNMTRFGSIVANNKAELFGNDSSQVPSQLSITINLIDILPRFTVLEQENLLIQQILIKPYQVFTNEYSDAFYVPNGQQISQYQYFDWNKGEYVPYNLHFRIVALDKLNSIQQDLESTYCDISGRLLMESGENEFTQNFTNIKRIEFNKSDYNLDNLIIYLDDQLNKTLQLQFQCNSIYIPIYDQNKEIVSYHNNYYLRINLKTLPCQMGEIKSLVDSTCVPCNVEQGQYSLGINSNTCLYKDDATTSDIKSAQLKLKAGYWRPYFYTNEISECINLIENCLGGWKEGDTSCYEGHIGALCEECDIYNIRGQGNFFKITQDSTCAECQDSKLNIIMTIIFGSLWSIFQIILTLRSIDQSNQLFSQLKLRQRLSKILFKLDQDHQSILIKMFINYLWIFALIFTFNIQFPIFFGFVDKTSNPSIFLATSLECYTSEMHNIQIIYSRIIILLILQLKLFLIIYGGYEISSLTKNKIFNNSILSNTAIYLYISNYAEFAKQFTSLISKRKISNIDFIQGDVSLEYDTYTHQQWIMSFVIPGYIIICFLVPFLSFILLFILKNKLDEIKLRKHICYVFNEYKQENYYWEFIKIWQKIIMIFIIIYFETNIYLKGSLLGLCLLLYQAFALKFQPYIILKFNNLDLQTSQICSISIFIAIGKYASEQQNNFNWSVMLQFFIILLWMKLCYPFIKNILLAYFKIYKINLLTSIHHIIKYCLPNNYLTKFFERKLILYEKKQLQINQNFKKLKQYLFSHTSVKSKKNLKNILLFRGASQQISPRALETETLRYTDSIILNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MW10</accession>
    <name>A0A8S1MW10_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA pseudouridine synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0610069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8078994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MW10"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF4">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8078994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15711" checksum="F8CEECB709A09A92" modified="2022-10-12" version="1">MDYNINNRIQQLNQHYMMLLLKQNLVSELNADSIKKIGVSRGARTDRGVHALCNYVSLKLQIDQKYCQSMTNNKLVDKWNINWDLLINELNSQLPTDIRVNGIKHVTQSFNVKNQADQRMNTQLSQLHFKKRIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MYD2</accession>
    <name>A0A8S1MYD2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0690121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MYD2"/>
    <dbReference type="OMA" id="FMEFVNM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035848678" evidence="2">
        <location>
            <begin position="17"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="182" mass="20532" checksum="DB4D58DE52321D2A" modified="2022-10-12" version="1">MYKSLLLCALLIATQAGHVRKTHGAVHQKRVFNSAFMEFVNMGDSDYHLNPKEAQHWAQITSEETKHDKKAKKHASLVQTNEQYVPGVVGQVTDLSNNAGVYSYTVTDLNGNILEQDAGDHLPKQFYNAYLQMTQEIEKGPAMIKLQTDLDNIIREDQLEQESKQEQQSQALEGTALSEQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N3V7</accession>
    <name>A0A8S1N3V7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">S1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0800044</name>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1110045</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8099950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N3V7"/>
    <dbReference type="OMA" id="KMEDQEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="3">
        <location>
            <begin position="21"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8088017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8099950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="188" mass="21330" checksum="158724200760E275" modified="2022-10-12" version="1">MPKNKGRGGKNYRRGKNENLTKRQLETKDDGQDYAQVIKLLGSGRLICLCLGDSKQRLGHIRGKMRRKVWIQNGDIVLVALREFQDEKCDVVYKYFPEEIKQLKNLKEIPENLEEGGGDNIGDNVVFVNKEESSSSDSDLSDSDSDDDSDSEPKQPQKQQPQKPQPQKAQAPAKDNKEKITKKDIDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5Y7</accession>
    <name>A0A8S1N5Y7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0670263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5Y7"/>
    <dbReference type="OMA" id="CNTSDIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="250"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="295"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="62276" checksum="B1B9B2F9D39EB1DF" modified="2022-10-12" version="1">MSKQIVLENQNEFNCQNNHNQQIKLVVLDPNLQKNKRLICNLCLENFQSNYPTMGFQKMIQIINDLQKQKMEELENKTTLNIKQLEKCVKLTQELRMQFNRLFDSLIRIAEDWSKDLELQIKECYQYKFEDELDDYIKKQNNSQIANIKLIKNINRSWMNKLYNNLNQYNQNEDKINFRELKKQFMKIINQKESQEDEIKLNLIDQSVKQSIKCNAISFDSSGQYMISTEYQNIKVWTFKNGKIKYLQTLYGHSKWIQCLLYSKKQNSFISGAGDKTIRCWKNKNINDWISSEPYKQHTDWVMCLEMNKNEDLLFSGSSDYSIKVWKLDFNQNELTYLYSLNNHKYYVISISLNQLENQLVSCAQLKNQIIIWERKEKNTYEFKYVVKQSIYNYGLKVSFITENSFIWITGDQGKDMIYFFELIEGIYQEIQHKSIQLTNNNEDYDEYFFPIIYNKQRNLILVRHKSQIYLIRNIDNGNFKIVRQLNCNTSDIYGTITDDGQYLVFWDSKNNGYSIYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7Y5</accession>
    <name>A0A8S1N7Y5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ABC transporter family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0820062</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7Y5"/>
    <dbReference type="OMA" id="CLSANSH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001172">
        <property type="entry name" value="Cystic fibrosis transmembrane conductance regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="646"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="687"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="769"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="72"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="378"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="646"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="955"/>
            <end position="1176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8088750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1176" mass="137030" checksum="08BCDDCD3039A99C" modified="2022-10-12" version="1">MDILFVKLFKRFKQIKETLNQDKSLKIQRLPIIQEHLKIKDQNLDLTNIQFNTVLSITYFIFFKEFKYQTMIVFIFYILESISKNAVSIIMSLLISNILNQSPNSIYIGALLILVSCIANISRHQAITKSMEFSAKSRFTLLNMLYQKLLKVQTQTIDTGKLIGLATADISMLEYYFMLIFQIFVMPISFIFGSFILYIRFNGLIGLIPLFIIMLIYPLQIFIQKQSIQYTKLYKQEQDKRIKLQSEYIDNIKVIKMYNVEYKLNERILEVRKREQQYLFKSQMIQLIDRSLNFFAQIWASMIFIVILYLFNYELNSASLIATIQQLQYFKVSCVQQVSYGIQSYLNIKMIFQRYIDIFNNLKIKNKQIQIHQTPQLIKFNDFSIGWKQDQSLLKQISIEINPKDFILITGKMGSGKTSFLLSLIDDGPLTLCGSMTQKEHLTKSYVEQDPILFEDTIQNNITFGKLYIEELYQKVIEVTCLKEDILKMQQKDKTVINDRVTTISGGQKTRIALARALYSLSDILILDDPFSSLDVPIQIQIYKNLIVFLQNYHVLQTNKYPAVVLTSHSQAIINQFLKFYLLQDGQLIEQLNENFNDFKLINISKNESIVQQQTIDKNQVIQNKVQIKKDNKFLYYFRYWTKNKILSIILVVLVVLLNFLSEILYNCYYNGISLIKSDNYDEQIRYIIIICILAFVNNYVKYILNSYGCLSANSHIHNNMIQSISQGKMSYFDQTVGSQVLTKFSTDLSLADNMISVTLFDFWELGSYFLISLFSLVILQTHFIYIMIVTILLNQYILSNYRNLILKSQELDQQTKGPLLDLFKKTITGLRTINVFEQQGYFLQQFNIATNNAILSNTTYYYAQRLFGISIDFLGLIVQQCGIIMICIYNKNETFTQALLLLMIFNENQQWFLRQYLSFITQINCVDRMQDLIGIDKEENNKNQKKTINLQGNISFSNVYLKYNQDYALNGLTLNIKQGEKVGIIGRTGAGKSSIISIIFRLYDIDKADRFEIDGFDIRDICPSILRSNISIIPQQPIIFNDTVRFNIDLFNQHSDKDIMNILRDVRLYDYVQSLPNGLNSHFGELSQGHKQLISLSRVLLQDRPIIIMDEATSNLDSETDLLIEEILLQKLKNKTVITIAHKTQSIRNYDKIMLIENGRVSKVGNPLEILNNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N937</accession>
    <name>A0A8S1N937_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">F-BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0770066</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N937"/>
    <dbReference type="OMA" id="SAMFNNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF7">
        <property type="entry name" value="NOSTRIN, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="114"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8086726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="506" mass="59828" checksum="D7472A4229F7ECC4" modified="2022-10-12" version="1">MDFSPDISERIDTLSRKCLEKKKVILDFISLLQARAELEEYYSRSLEKIGESLTKLIHGKDSLKDLFIILRSYFHIQSEQTRTFARQIKNDVIQELEQHLIKEDGWQKEMQFLKQKHSKEIRRNIESLNQLRDEYIQAINEEKKYQVNKQIQKKLSYKQSEIKLQHFIAFYNQYIESYFQDIAIVQDKFSNIELERRKTIQDSGIKFFMFEISVVRNLQYDLNGISQKIEQYNPKNELNELFKDQPSDKPLLSKLNFENYKSFIHSQLNFKPDNQMTIKKKKPIFDQTTISENQIESTLQFKETNQIYMKMFQSAMFNNIVTDETVTQLNELLQQTQPAVYVQSLDKILKLRLNQEDGLQNTTDFQVSPEGYVSLHKLIITCLDYCNKKNDYANIIIDAYQLTRSIYKLIQLPNDNQEIKMSVFEGLTNHAVLHNSQIWINYAIQLQTLFANNTSVFAENNVTKEQTQKQIDDYIESLQNIGCDQQIVEAVKLQLNYNVIQESPIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9T5</accession>
    <name>A0A8S1N9T5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0780006</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9T5"/>
    <dbReference type="OMA" id="YISAVMW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8087066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="417" mass="49156" checksum="F87A174A8ADF53E3" modified="2022-10-12" version="1">MKVCQICFTNESFITPCNCQNCIPCLINWFGIKNNETQIINFNCPNQHCSYNYSRQELQNICKYPLYEQQLSEILLKEYLKNQDIRPCPNGKCRNFGFIDLNSRCDQLLQCTSCQTKWLDLSISSQVVQLEYGFSNMYFNIKDFIYCFLFTNECPQCGVQIQRNGGCQHMTCKACQHQFCWYCKSRWNGHMLRLCSFSYATKMGIFSYMIMSLLQTIGFLNSVLFLFSLPFKWFTFVILANSIPFGFVVGIVYFCYGFSHLYYQIYKKGTLEVIISSVSLFIWYHLHCLIAEIFELSIQIYISAVMWEGIIAVVIVCYLFRQQLTWIVFPISAMSLLYYFGLNGLCLLCWGLPIMKKQFANNKYLFQFAGYLLFFILNIFNLQEQISQNKISYIFVLNLVYILYIEKSRIFDQISFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCU5</accession>
    <name>A0A8S1NCU5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0780142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCU5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="102" mass="11890" checksum="533F7E5FD5C9915D" modified="2022-10-12" version="1">MERVVLSCYQLQKYWDQIQTCFVQMNVLQGNIAKLEKINKHSSNSYLVLRQLVENVLLKCQYNNDIILNKKCQNSLLIIGGLKENEANFGEIYNGFLSNCWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEL9</accession>
    <name>A0A8S1NEL9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0810014</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEL9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035896995" evidence="2">
        <location>
            <begin position="16"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="54"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="363" mass="41395" checksum="CF548852697B2C28" modified="2022-10-12" version="1">MRTGLLICLILGVLSVELNMERHSEEVLAKLRESKWASFIVDFAEVELGSGGALTELVEAINQLIDQLEEELDDIHQAYSRRTDEHNRDVTRYEQEIQDADRDIFNGEDFIDNVLIPQKQRFQDALAQLKTNIEENRRILDSETVNRKKQHEQFLSNIAEINEAIGAVDESLGLLSQITNPSLVQFKRVQTNLGRIQNSFQNHSSFAPIIKALLELATEQNFTDQGSLQQLVKIFNELRVQFVDTLNQETADESAAETKFAERVAQLEKEFAEFQRAVLIKNSEIAANEQKLGETIVYVGQRKDDRATLQAQLQAENDNYASETDLYNRTVTEYNKEIEISKQALGLLTQPSFEQYVKSKVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NG84</accession>
    <name>A0A8S1NG84_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0890021</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NG84"/>
    <dbReference type="OMA" id="YNESQNN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="2">
        <location>
            <begin position="17"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8091708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="681" mass="82266" checksum="969E8F0267215EEF" modified="2022-10-12" version="1">MEGKLNLYLDIFKQPSNKFFIIKYKTRGQADQSLNNKVIFVQKRFDLQLRNAAQVCEQVGVFLSCLDQKSFIAFGTIQNEPTDFDYLTVIQKLTHQEEQQQNLSKLQEFFQSFVKVEWIFKGVLNFDKTQNIKNDLNEMKPINHCKDVQELTYQTAEYLFSLLQKQKPYKPQPQQDIGIPHQLNKILRENFGENNSEKQKSSKSRSRLREKDQDNDKYKDQDDDKDKDKGQDKDKRKLSKDRKKEKKSYDSKSRDRRSKDRISKDKKQHSNEKQNYREDKSLDIQEFIIDDNKDLRDFEKQKERQAREQEQIKEKDQRERDRTKEQIDREKEKEKKQLKELEKREREEKEMIEKQKQQERERDKDKDKDKERGKKKKDRERDKDKQKDTDYNRDREKLDKGRYNKKDDLKYRDRNESKHDYDKRRKDKERDKKNRERSIDQSYDKSDRKDRESKKEKERQRGGDTERERDYNYHKKQKDYRREKKRKRSEERLTPRSRSDERRQDHNQNKNKKALDIASLSELQRELYSSVSLQSHKNEKQQQDCELEVSSKLSKQKEEVDLGVILDVLDSPKQNDQQFQPLIQPVMITYNEQAVLTKQSELQQQNNHSDSLKRILVVKQEKSQSILDIVDQQKSQQQGSMKIIKDAPVKIIQQINIQQSYQESKQGSGNSIKDRIILKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGB5</accession>
    <name>A0A8S1NGB5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0750050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGB5"/>
    <dbReference type="OMA" id="QGEYSWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8085754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="40873" checksum="2D516DF8D3FDADF7" modified="2022-10-12" version="1">MGNNCAGQCAGCYKRDLEIVKSEQQQILSEKPIESSSKRSSKNNVQQNTVQLGSKSDLTQKPITKSLVDMVEDKQKKTKAIVKIQSHWRGYKVRKEVYSMIKSQTSVSSQVSKSTGSYFDRLGNESKSQKLLKLYNGQWLGNERHGQGIQKWPDGATYEGEWQHNKANGKGIFRHADKIEYDGHWKNNKASGYGILKSPSGAYYEGEWEDDLQHGFGKEKWADGSFYEGQYVKGMKCGKGKYVWSDNSYYEGEWLNNKIHGQGVYHWIDGRGYIGQWKNGMMNGQGEYSWIDGRKYIGNYLNDQKHGYGVYRWVDGKEYRGEWQFGQQNGEGTYITVDGKSRKGQWQDGKLVKWIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NIA3</accession>
    <name>A0A8S1NIA3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0760175</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NIA3"/>
    <dbReference type="OMA" id="NYSKNHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16306:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_7, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16306">
        <property type="entry name" value="TRANSLIN-ASSOCIATED FACTOR X-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="586"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8086414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="672" mass="78838" checksum="06E4271FD296E241" modified="2022-10-12" version="1">MEVLQHIIKTQGTNVTIIDLEFKQIENLEELLPQLANFSNLKELNLHGNRLAYLPDDLSLLDTVEVLDISNNIFSDLGQVVDALSTMPNLIHLEITLQSKEEEQYILECLTNLQILNSQKVNGDQDGGDETLEQQSEQQSERSISAVQDITLQQYDLEQMAILYDSIREYKKEDQEDKQFDQQIRTIMLDLQSKLKQNNPDHLTNLYILTAKFNLYEICFKSIIKYFKVNDKKLDVIITKIHDMHLQIFNDMSNVISNFKPQSKNQSLINDNKKQVNQSQQGDKTIENRLRLELSELQQQNVELEKENKRYLDLLIKHSKGDRASLPNSEHILKQQNDSYQSQNYSKNQSSSIQQNNAIFQSKQSQNLNSVPQQINVRHLTLKQLKDVINEIYESKQKFDQKCADSKLPRETMEQHMYTYLNQKYGLKSLIIEWATSIINALKKYSAEDNDVAVFGKILRNECDEEFRFVQTQVKNTMQELLKMYLRGKFPLKHQAEIKEMLNQRINGQLYEEEAVDIIKYMYNQEDSELLLNKLKSYYIVPQKNPDRRLTREEQLQLLQEKDKYKLEYSVFQKIILDFQLKSHEKYLKKFIMTFKQMDTDANGIIDENEFRNLIDILNFGAEDLDIQKYLNIIDPYSHQQITFSQCVTLFSSESVPGSNGQLQILQKISEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJV3</accession>
    <name>A0A8S1NJV3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0880198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NECCFAI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="124"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="39878" checksum="0976383C98229686" modified="2022-10-12" version="1">MIINGQLHSPYLLAGTYLVQNLRQSILMFIQSFXQYSISQNECCRAIDINKDCSILLAGCDKEIKVFEFNQGIMKLKQILSEHNNDVHTLNFMKKSNQFISGSYQSIIIWQQNQNQQYVTQQILNGHNSWIYCLILNNNEDLIISGGSDNTIKFWMKKNEWTCQQTIKDHYNSVFGLSLNQQQNRVVSCGNDKQILIIEQQQQNKEWIVTQKITVELSTLRVCFIDNDIFTLSQCGQNQISIFEMNTINKQFTKTKDIVVKCGSDGYCLFPQQYLNSKSILFSKNGEYVNLIRKKQNGEYLTEQSIHFSTNVLFGRISDDGEYLITWENKSKEIQLRRYKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NLX4</accession>
    <name>A0A8S1NLX4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0790029</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NLX4"/>
    <dbReference type="OMA" id="LLIMETD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8087624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="268" mass="31087" checksum="826BF75F9287F672" modified="2022-10-12" version="1">MQDKSNTNSSVQESYSPKKPADGGEKKKRSRTRKYSDAQREHKCECGKSYLSYPALYTHIKQKHNGQQSLKNLDEQRLESTFSESQTQDIFATLANALGKEEDKPQASLNIQDMYDLIMWEVQLQIMETENKEIKFDYYHELIKSTQPNDVTTPQDAFIKFIHEVKGVLSFDQMKFIFLFVYSFRKLLLDCPECPTSGLVEKANQLICSQLDKVTQSIQQLSNRNYIVPLCRSANLREIMIQIVLGFCNWLYARHLTDDKLLLKSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NNG2</accession>
    <name>A0A8S1NNG2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EXS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0970047</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NNG2"/>
    <dbReference type="OMA" id="ETSHFYT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF103">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 53 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="489"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="513"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="559"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="583"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="7">
        <location>
            <begin position="450"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8094817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="757" mass="90648" checksum="75C2A7D489CDA439" modified="2022-10-12" version="1">MKITKVVEWKPIYIDLNLMEMLLQPYSLINHFNQDKKKSVNKVQTSSFAYNSMLVQDINCLNELEQLNEKIVERLCRDMNKCDQFYNLHQQQIQVKLRKLIYNIFIYQDLQKIENIKSKLYILKQYSEMLYLECNLLEQFAQFNEIVIKKVLKKYKKQVFIQDPYYNLQKMQSVYNELQIFNNRQDIQKIQVKLKLLYSRCLSSKQQNKNIRKLKYHNIYKGFTRKEQLLIGLYLGGTFCLFIYIISKRNQLNNNFYKNQFYYDLYFPMFRGIGLMILYYWSLIFCVYCWIKGNVGYRSIFNFQYHSSSINQLIKRAALITLLYFTVLIISLQKELYLEEENDKKQYLLDYITDKIAYDPAIGPLVLWIIMMLYMIWPSKKYLNAKGRKYFWKIVYTSILAGFFDCPFVNGWSTDQLLSLVLMLKDFGYTVCFYIKYFQNISDYQSQATCGDPKNLQIGLIVCLIPIFLRFVQLGRCFYDAGKITKDDIFVVLIYVEVTMVNVFSYLSQFGNSYFIVWIISFCTLACHAYFWDVKKDWGLFQINSNHKHLRNQLAFKPIFYYIAIVFEFFLRFAWILSISPNMASIIHIWSPLFSLIMAILELCRRTVWNIFRIENVHIQNMGDFKAVYPIQLPFESLIDQQSYVNIKELRKTQIDPINISLLSGTQNQQSVSGQYFQFRNSLRSDSLEDESKLNQLQQQEQDLIAETMFEDRKRESILTMRYSQFKIDHQNEKQTLSKQELLRDLSIIQEMIKKMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NNN1</accession>
    <name>A0A8S1NNN1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0890155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NNN1"/>
    <dbReference type="OMA" id="KLKRNWW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="106"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8091976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="342" mass="39757" checksum="2DD2E1793B9B000C" modified="2022-10-12" version="1">MGSTCKNICKQQVAEKELNIKVMKKVKLKQKQHSNGDSKTDPYCSPQLLIKEESIGDESKFSHTQNDQPLFVKTKQINSIAVPTDRKNRFLNDQELEVKYLIKQNLLKEQEELQRLQIEFEMQKQLQQEEYDRKQKEKEKSQQELENQFSFQNSFKQAQIEQPYVQSSSNPYLPRQTSQFAPKKADCDTVSQKSQISKTPTIPLKDQQVSPKSDIMRKSALKKIKQNKEEIASNNASIGTPKNETKRKQQSQDAFEKMFSECCSFDERSQSGSHKTVKSVKSILKKNTSLSQRSISMGKNSIKSSHTVFKIKQTKKVRFSNDTNFNNERKGVPKLKRNWWEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPW7</accession>
    <name>A0A8S1NPW7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0930059</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPW7"/>
    <dbReference type="OMA" id="FAYCVYG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806:SF1">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Longin" evidence="4">
        <location>
            <begin position="65"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="5">
        <location>
            <begin position="141"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8093409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="202" mass="23562" checksum="F26D33AD73DA442E" modified="2022-10-12" version="1">MRIIAMYLLKYHPENPLFISQNYELSFVNWFQRPFFKETLNFGARTCAARAKPGTRSAVTNQEAHAVSYICIDKNNLAALVIADDQYPEKVAFMVIQNMYQEYYRQYNSMFLDTIVADQNINIPKFEEFIKLYQDPKEVDKLLKIENTLNEVTMIVHQSLDDLLKRGETLESLMAKSKDMSSVSLDFYKNAKKTNNKCCSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NVI5</accession>
    <name>A0A8S1NVI5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0930149</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NVI5"/>
    <dbReference type="OMA" id="NDMEYEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="475"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="503"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="609"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1195"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1195"/>
            <end position="1209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8093535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1216" mass="142851" checksum="A37AC7654E81B4AC" modified="2022-10-12" version="1">MQNKEIAFLSIESPIHSQNVISPRFDELIPIHSNENIDDQPIDEAMKLKRNIFDRKNFRALNDGHKSHNSNDDNSLSNINESLVEDGSVKSKVNSLDHQSLSQKQFQNNSQLQNAQCGQIVDSACNLLSKTGKSNSKLEKYQSFVQQFEIQEDKIQRIKTQQAQKQIKIKDVKPKKFSGRREGTVGLNEIWSQNGLMIIRLVSRFIQQLKTKTETIKFRLLTHKIYSVIGDLSSNFEFILVTNQIKQKPSLFLILKYNFQKQATKYLHYLENFQEYLSRNIIVIKPDSKFKIIWDILLLLFIVMNIFYIPINISFDITISGIFEYLFDLLPSWIFVVEIILNFNTAYYDKGLMHEDRKSIVKHYLKNNFFWDLIVVIPFLISNLNIPFVRYTLLLRLTRLNPLMESIEEMLNLEENIQIVVDLFKLIFFLVLTGHFCGCAWHFVALTEYESFGITQTWLTHYDPNAYNYDWFDRYIISLYWSVITTVTVGYGDIVPVTTFERIFVIVVTLLLCGIFGYCISNIGNIFKSISDKKTIYKFKLRQIHQHIRKRGLNLNLSLKVKKYFEYYFKLEQEEDNHADIFLSQLTKHLREEVLTDLYSNTLKKSRLLRDNFNEITINNLCQFVKEKKVLPEEVLYSRFDQPKRIWFILSGSLEYIADHKNENDYYEATETFLKKFTAGAVLGEREFITQQPYEYKARALKFTQMAYIDYDDFINVIAENDMEYEIFCMKRDRLLLYPAFKGSGNVCEICEWTHNFIQCPFVFLQPNTNKIANKFTSVKLNNRIKFPYRKPQKSRSKETLNQVQECALRIIVGNLTEQEYTDEYLISLGFQLSPNLDDMSPKNYESSQNIHDSKSISNSDKQNSILQKNTRTIVGITSDLRESRDLDLSKQMRKSIINFKRLQFGKERTRAIQFIKKDNFYQEKIPETLEQSQMLIIPAPSQIQNQNNHTAAAIKQYQQKSRRSSNLDDIGFIQVSQKLGNLAQQFVQQDNENSKYSLKQMSKNSEESEMKDSIKIIRQIENQEKRVPIGSDQRRKIKKTTIQMGYRPKRRSYQNQQTKNLSPVTQNQQQIQQITISLDQNKNRTSVIENKKNLCQNTQLTSSKLIEFKGEIKEGSQIDSLQQKILDIMHQNVNLEMDICKSTLVYFPEHNIEAILKKIELYYSQNKVKEKKQYKKIQSNRNLFERIKGSKNATIKSLQNNSKSQTDVQKQDENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWA7</accession>
    <name>A0A8S1NWA7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0940050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWA7"/>
    <dbReference type="OMA" id="SMAQSHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="3">
        <location>
            <begin position="515"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8093775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="762" mass="90124" checksum="3BB260582E8D36B2" modified="2022-10-12" version="1">MLFEQYKDYEYDRKKMELEIEYHTPKTIFVKAFGIELLFRDVWPYLIDDDRKLKLVILNFIVQYCKTMIPKWKINEKYYENFNHGNIEKPNEECSHTFYFFQYLDEWSLCIYSTQEKTLYSYVLSESTQKKIKTIFTEKGIKVDQRINIQQSVTIAEEDTIVMVKLLYQICKQQELIFEFTQEMAIEWRKMVKVIDLFLFELHKQYKEQMEIQQNQSKSQTHSNPNLSIKQDIKSLEFNPDKSKIGEAPSSNQSLKSIKQQASFEKSLSISREAHGQVTDEMIQLSKEEYNCLIDNFKQKVIQELQQIEPQHPTVFLQKYLKGNGDLMVKKDDQGKDELVVKLKGEQLQVLLQQYEKYYQQQINNKQFEKQQEQMKQQYQQYIQFQQLLNYYYQHDPPKYSKLVTEYYNRLNEQTLQKLKPLFPQNIQQQESSQNNISQQSLKVPYSPQIQSLSYSKSSDRYNKPINPLIQPNFNVSKSFSQNQQLQNSINLSKSIDPQFKPSPQNTVVCSRYNKQITYSEINLLQNKGEMTENLLNFYIRYLEEKQSSLQLGTLKQLKLRVFYLSTTFYKTLIKEYNNPDSISYQSASVYTSKHIGKDNTIFDKFDIILIPIVIASGLETILININLAQQTIYFYDSLINGVGSQSMAQSHIAQNGLLNNPFLMCCLRFLEIEYNQKLMKTLSLLKWNIVYSPHEKIIENSKTNTMIAFLMTQISKGIQVEKTFTIQLDLAKFTSNLLKLFSQLGITQNRKNELNFEKMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZQ4</accession>
    <name>A0A8S1NZQ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">P-type ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1010033</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="MEQSIMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1028"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1061"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1111"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1145"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1177"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1220"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="6">
        <location>
            <begin position="1009"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="86"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="567"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1261"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="574"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8096321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1313" mass="152133" checksum="83DF4C4F152CED39" modified="2022-10-12" version="1">MTEEDDNIIAFEEDDDNQPPKAQPKISTRFKHKQVRQQEISNEQAQEASEDGPVINFTKLFKTSKEAGTAQGIKVDPTYVAPMAKSLFLNPDENQEEDEDPGVLKLEDPEDQPLPPEEGDVFIKSKSNRSQRSTPKKDESILDVIQKYDGKNIENSLEFSVHPIGKGTTEQFPASQENRDELVDTIFSQQLKIGIQSTDPKTDFYGISMLTQKGSLFQNALKMSLKRAFMTPILPFIIIITILDYTMTSVNQFQFFIIYILLLATLPTLLWYRVYKQELELEFKFDRQLVSVFDFAQKRFSSRTIIRLFPGCIVQLKKGERVPAPMIILSAEGQEHAQVNFGDDVKTYKVAAPKLQEPDLTYIDGQLEFNQLPIFSENTKARLRLDLVEPIDVQLNQENLVDENGIVESSQLIGVLLHSSTRQKYLREALSFNLPLYLNPLNSEFFSIGIAIVLSITFSIAGSIWSSSNKDMHIYIREQSNYNFISLLIDRLIIFSNIVPIFVEVFRPIFLLLARKKVNGDIGMDPQRSGQLTKLNSYIALDNLANTKFVNYGQVLNEPRLRVGKATLLGGPQGEKKDNEKNNDDVKLQGYEPKPNKEIMDDQIQVNQSTAFEQIDLEYDAQCDDKERSTFVFERALLTLPYKTLNPTQLAIQKYCQLGECVQFSYDDNQRSVLLPKEVLRTYTVKLIVNTEQWDAYLYEMPLLEEKDLESDDEEASDLQQLQDRIKKDRELFEKMNSKRYIWVLQADIESTLCKEHPQLSQFMSGKAVFEYTFQEQEYQRLYKQLGSSAFKRKYIQLPPFAEEQLMTQIKPCGVFQFQTVAHHNFHLLLDLNLAIIRQWFFIRDVERDQTLQYLKQYMECIEFTKDDLHNEMNSVKSQAVPLFKQSTREFKVFDNLYKIDDKFITLIINQEVLDQALSDGEIRSQFYEVIMIASFVVFHDLSKVQIEQLITFQKDQSKNQAIVSVGLHDEDLCLCKVNTIRASVQSQSAKNPSLFSAMSHIELVNLDGLSKLILTHARQTHVRTAGFLLFILYKGFLITMCEFFFSFFNGYSSTQLFTTSFHFMFQTYFTPLYGFLIFSFYSDVNHRVIRMFASEFIKCQRDYYFNKKKLFLIFFDSVYQGFIIFYLPANILQYANNSEGIIEGFYYISLVSYLTILLTTQMQPILYLSTLSKEQIVVIMFNITICILILFTTCSSETWSTSIPQYIHHLESILDSVKSVFIVIFLSCISLTPTIILQQFETVSDSQLIRMIHYYEEKEEIEEEPKQKPQEQKLSQQVDTKQLQTKGLEQSIMIEQTSQDQINMSQDNLLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1D6</accession>
    <name>A0A8S1P1D6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1010111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1D6"/>
    <dbReference type="OMA" id="AIFKNCY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000093">
        <property type="entry name" value="NLI interacting factor-like phosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="1">
        <location>
            <begin position="27"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="275" mass="32039" checksum="46ABD0D36496EEBC" modified="2022-10-12" version="1">MAIFKNCYEHFRGKFSKQYMPAQSPKMHSKKKVLVLDLDETLVHCEFKENNNFQHETLLKVNHKGQEYKIYLRTRPHLNQFLVEASRDYEIIIFTAGYEVYCEKVLAYIDNDRLISDYYARGSCQFINGVCFKDLSLLDRPMEDIIFIDNNPNAFDRCQENGLLIPSYLDSDDDDCLLRLIPFLKYLSKKKDLRPIAQHINSYENNIGATIFTENQKSIDIQQEAMDEDTLSEGNVVKKDNDITDLDIKHKKTQTHLEAVCKVNNKIRSATLFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P3H2</accession>
    <name>A0A8S1P3H2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1040109</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOSR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P3H2"/>
    <dbReference type="OMA" id="FDWESFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048219">
        <property type="term" value="P:inter-Golgi cisterna vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023601">
        <property type="entry name" value="Golgi_SNAP_su1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094:SF2">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094">
        <property type="entry name" value="GOS-28 SNARE- RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8097572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="257" mass="30520" checksum="452F1BAB4015FD27" modified="2022-10-12" version="1">MNKEQDDQERQPILIEKDNQYFDWESFKKQYYQQENILQTALKSLNELHSDISRKLIQLDKSDPICEEDEQENRKQFQSIDIKKYQDQFDDLNLIMSTMSRMLNEYAISPEEVQAKLKNNMLRRFKEIHQEHDKEVLKLKQAMKTNIQKYELFKDAIKNQNTQGNQKYKRDYQLNDFLSRGVNQASHVLDMASTIHSSLEDQGYRVSSIQNKVTEYMGQFEGINQLINGIRYHKNKGQILLMGLIALLVVIILFKLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P487</accession>
    <name>A0A8S1P487_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1040178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P487"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="65" mass="7871" checksum="F1623094DCDD342C" modified="2022-10-12" version="1">MISVILRDQESKRIIKYLNLNITYLAKYMNKENEINVLIKNFFFQANQCISQITQGYFYLKLKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5N1</accession>
    <name>A0A8S1P5N1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1060163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5N1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8098313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="88" mass="10181" checksum="631B8531904E8F3F" modified="2022-10-12" version="1">MEQRQNTTCLDQKSYISSSLANNLQHDDCCQLFENFIKINITQSNAQPYDPKCFTKQYNVIVQGVVFDILSSSDIENQLKKEKIESEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PC42</accession>
    <name>A0A8S1PC42_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1120102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PC42"/>
    <dbReference type="OMA" id="FMEMNDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="604" mass="70456" checksum="C91A4BF7F13CD821" modified="2022-10-12" version="1">MINSLQKSLEKIDIFGQTITLNMNKNAYYTTSFGGCSSILIICLLSIIFFSNISDFFSRANVFYDSQITFSNDPHFMEMNDQNAMFALSIDQANFTTNPFFNISVEQRIYTRSLDGSLNKSTIQIPLQPCTLDRFNNVVKEQGINIDFNDEFNYLSMNKWLCPALNYTLQLEGTYSSEIFKFIKIVVTGCKNNTNSSYWNPQCADEQQKTNYLKSEGQFKLQVFQINSMINPQQAQNYKTMYLDSDMYFSFVPQKLVRLANVYYRQYIVNNDESLLPYQDIKTEEMIIRKAEDFRDLTELGRDTDTIFATLYLRRSPFTEIINRNYQKVGDLLSYLGGFMQILKVIFGFFIAFYNRTSMLIELSNKLYDFKEVTNRSFLRKDAISQKTDQRGSIPDELSNKLQNLQNSDLNKGDWRQFINKLMERTSPIRLNVKILINQMSCGYFFNNNNSEFFTKAMLKINSELDLHNILHQLQEITKLKTVLLQKPQIILFNFTPKPIITLGQDHTVPTRLELADDINKQSYIKSNLDQLNEDLFTSLQEAYKTVSEEMEDSPNVNPCQKTINSKLTKQIDQELGKILKNQKVSFYKIHETNNINEIQEEYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PEM1</accession>
    <name>A0A8S1PEM1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1160130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PEM1"/>
    <dbReference type="OMA" id="MPKIQPD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="151" mass="17379" checksum="D7A0082A2ABD0650" modified="2022-10-12" version="1">MNTNKLRISKSLIRQRQSPIPPLEPKLFIGSPLSQSPPQKAMHQMTFYQRLKLYYKSQNTLTPKPQNPPPDEQGTILRLISVHDVIKSKKKELQSRVSKVVQIQTSIHKRFITDMPKIQPDAVTKKIRLNFKSPINVLNKSNSFGPWDSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PES5</accession>
    <name>A0A8S1PES5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1140058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8100998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PES5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="411" mass="47530" checksum="293F133D106494EE" modified="2022-10-12" version="1">MNQTYLSSNNDITQIDYDEQEQDKIQNPNDSQRVNISLSEKNQERIENPSFFFRNKQIELEKQSIKSQIDQMIYHLNQMKTMCERKIDKCIQPQKSLYQNMVYIKKFTFYINYLKLKWNSFIKITIIYILISRCLLKEANFIQMKIMKETLNQYHRLLIIDINIHIIIMDKSFANSSPLNTSKSKQMYTFSKAKRWIDIKDNLCPPIYQLPTTLSKRAAGIGYGKKINLTHESITPAPNFYSLHKNQEHGWTMGLGREDANKYESIFLGLVQNTPGPGTYKFKDSISPVRYTMRQRLTSRKDQDPKPGPGHYNLPSSINSKGIYPLSQYRNSGAIVLVPPRTHSERKVKDNTPGPGSYKQIGNINPLGTYFCSKFGASKCNKFPRAQRSMSDHHNSSPGPGTYKLPSDFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PG20</accession>
    <name>A0A8S1PG20_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1170106</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PG20"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001215">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:002786">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 410-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849">
        <property type="entry name" value="LEUCINE RICH REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="416"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="779"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="371"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="56"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="416"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="452"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="779"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="829"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8102055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="841" mass="98935" checksum="BB1C7AD339A96D3E" modified="2022-10-12" version="1">MSEEQIELQNDGNQEDQIDQEPQPEQNEEVADPEQKEQAEQPQEEEPEKPPEEDEETRRLRLEREEQERKEREEREEQERKEREERAEQERIAREEYEAKLKEDTAKFNELVLQGISGLSKTQTTYAYVKLNLAEKDIDRLFQINHLNLRYIDISQNRIVDITNLLPLKYLVSLNASKNEINTLSYFQDPEAFPYLQYLNLSTNKIKTLVPVQLKRLRRLNLIENEITTANEFEGHENIEILELGKNKLKSTDGLGNMPQLKELYLQGNELKDFRSLNNLPSLLKLNIRANKITKIKTPVIEFPQLYYLNLRENQLAKFDDFKKIAKIRTITTLNMLANPIVDEMGADNFKQEILMFYFHLVRINKVDITKEDYDEAARVLQERRDEEERKRIEEEQAREEARLAEEARLAEEAKLAEEARQAEGVKEGEGANEAEQPQEEEPEKPPEEDEETRRLRLEREEQERKEREEREEQERKEREERAEQERIAREEYEAKLKEDTAKFNELVLQGISGLSKTQTTYAYVKLNLAEKDIDRLFQINHLNLRYIDISQNRIVDITNLLPLKYLVSLNASKNEINTLSYFQDPEAFPYLQYLNLSTNKIKTLVPVQLKRLRRLNLIENEITTANEFEGHENIEILELGKNKLKSTDGLGNMPQLKELYLQGNELKDFRSLNNLPSLLKLNIRANKITKIKTPVIEFPQLYYLNLRENQLAKFDDFKKIAKIRTITTLNMLANPIVDEMGADNFKQEILMFYFHLVRINKVDITKEDYDEAARVLQERRDEEERKRIEEEQAREEARLAEEARLAEEAKLAEEARQAEGVKEGEGANEGANEVANEVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHL7</accession>
    <name>A0A8S1PHL7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T1170160</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHL7"/>
    <dbReference type="OMA" id="YNSIEHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000106">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000034">
        <property type="entry name" value="Kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000476">
        <property type="entry name" value="PAS domain-containing protein tyrosine kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000032">
        <property type="entry name" value="Radial spoke protein RSP10, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF298">
        <property type="entry name" value="MIXED LINEAGE KINASE DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM" evidence="15">
        <location>
            <begin position="266"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="642"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="198"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="375"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="563"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="386"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="429"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="465"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="567"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="600"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="669"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8102163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="923" mass="107123" checksum="CD9C09746494D5CA" modified="2022-10-12" version="1">MNLLKFLYGESSKQEANQKIQKHIIYSNDDEYQGEIMNDKRHGKGTYKFASGNRYEGQWKNHQKHGKGKLYYKNGDLYIGDWIENKKCGQGMHFYINGDRYVGEWKDDERDGQGTLYQADQNIYYGYFKMNKKCGEGYYFNTREKKYYLQHYEYDILLDSVQIEDVPSFVLEKFHIKPERNHIESEKSEDCIIKIEASPTVQNDSDKQKDLQPSGQSGQGESDPQLKEEGKSQLNSLGNSQQHGFLSLQVLEKLEEFESQKNMQEWQLEEVCNWLDQLQLSEYKDEFIKNQMTGKTLYTLTDNDLKQDLGISVLGHRKKILQSIEEYKKYYVKFMGGRVRMKKPLESLEERKGLYNSIEHQRFKSLFSYMEKIEEEQNEHHDNNKSDSCSDSPSANKRKKNNKDNPKSPTRSISNDSDQGNLSDNNKSLRLKRQKSKRSKKQQRKQTVDMLKSGESADDEDGKQHLTSPVQSPNKASNNVSDQKIEILVLNECSNIGEENKEKDFYGVNELSRQSTNKQQEISEFISSKSPPQIINNNEENITQLQSINQQLQRQKSEINGIKFQDQDSSSQESDVSGNDTSSESSDEEKYKNQKKSRRLKEINKKEKERDDRQQHHLPKKLLVMLKEFGINERVLIVFHELTIGQIIGEGGYGVVHKGKWLGQDVAIKSYGKRKSQGNLKYKLQMADFLKEVEVISNLRHPNIVLYMGVCIRKQNYYLITEYLEEGSLFDHLHKKKTHIDQKALMQIVEDIALGMNYLHGRKVMHCDLKSSNVLIDQNWNVKLCDFGLSRINKKIDHKINKGARIGTPNWMAPEIMRGEPYQEKADVYSFGMILWEIITQQIPYEGLSQTQIIGSVGYGQDQVPIPSNSNPSILLQLAKKSLKKNPDDRPTFADIVNEIQMGQKTDAKLKKQAIRQLMDFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHX2</accession>
    <name>A0A8S1PHX2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1190074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHX2"/>
    <dbReference type="OMA" id="IRYPTEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="199" mass="22851" checksum="0892C5FC319C0337" modified="2022-10-12" version="1">MLSSSQKSQVYKRTSYLTESVSIPNSARPQSSYVNTLEKKELRRYPTEWAEQVDNKTDNRFHHVIINQSKADHNPNLASPSKIFGLTESRRLNTQNDDLLSAFRRKNQSLSNLGCWQFNSTPVANQSQNTQGKASTNQKPQPMLTRKLDQSVPLRDLLELSKQLDSATPEEIQQLSRGYVNELVQLQQTITRSLKYIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIC8</accession>
    <name>A0A8S1PIC8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1190125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIC8"/>
    <dbReference type="OMA" id="HTYESRG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="26114" checksum="AECA6A72AFE45D59" modified="2022-10-12" version="1">MDSEDYHFKFRIVILGTILMYVKVRNQLENQPFQMVNQIIKFKVLAITFGKIIEQEASDELNLKTVGYMDDDTLYKIAYWEIPGKHRSPDTFFRFCLGATAAIYMFDVSKRQTFEKIEHWITENEKTEIPVKVLIGNKIDLYASNKGGVSKSEAIGMARKYGMEYFEVCSIGDTSIAPVFDYLFSTIIGQIPNPPTPLSLMGKGILIGKRLLNSSKYQLALCDIASLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIS2</accession>
    <name>A0A8S1PIS2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1190106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIS2"/>
    <dbReference type="OMA" id="YMMIKVL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051064">
        <property type="entry name" value="SEC14/CRAL-TRIO_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324">
        <property type="entry name" value="SEC14 RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324:SF83">
        <property type="entry name" value="SEC14-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GOLD" evidence="1">
        <location>
            <begin position="352"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="498" mass="58559" checksum="167ADF2B67CC4E3B" modified="2022-10-12" version="1">MSESPLITPLLQQYQVELQTKLNECFENNEVKGILHLIDQLGQDIKDEPQLQDLLNLQLLRKLMKHLSSTNPQQVRSSIIILDAIMQRKIIANRLLHQRGAERTMVDLANSLLNLQQQLRHTSLELHIDELLIGLQVKYVDKKQSDLSALKLALKQFQDNNSLFIKQLQIILSSCESIIDQYYLFGSPLQELIYEYLLMIRQMEEKQQVNFLTQVLGIYERYILDVQYTIQEMQQRTYYIKIEKQMILHQISNMYKSCAQLLNMILVLPEEIGLQKRIYMMIKVLYKYIPDLRIALMGPIQLVLRNLSLFLHKDVQEYKEITIFLYQLIHSSDYDEKFKQSLLDDEDLAYLRENKYFSVKALSYVDESQSVPSLRNLNIQAAFPCYAIIQAASIYCYSFMVDKPNSLIFWSFRTLDYDVQFGLFKLQTIEDLGIIDYVNERNGVKSLIKLQRIESHKQPIIGVTVISNPGLYRIIFDNSYSYLRSKQLFYSIHLLETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQD2</accession>
    <name>A0A8S1PQD2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1280028</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQD2"/>
    <dbReference type="OMA" id="NQGESIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="238" mass="28148" checksum="89CFA1189C3370CD" modified="2022-10-12" version="1">MDQPQLILSTKQNLLDLQAQVNQQIKDHCHQLKLIDQSNEKVIEKLLKQSEKLQMLVSKLDYYQIQPQVSYLDLILKHQYKYELKLSEDLELPCYRNRIFSIRFNLMYQNQIFINQNKIIVELEIWTYDELPKKVSHNNQGESIYKGCQQAFIKEGQGRLNKIQIKEVSSHFPRGQFVLIIVPVNDHGVIGNQKLGQIKKEWIKPLVLNEFVVKAKRFSNRHIPYYIRKDGTVSMKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQG3</accession>
    <name>A0A8S1PQG3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1260145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQG3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="50805" checksum="C60CDF7B66F2513A" modified="2022-10-12" version="1">MSFKTSIYLPLNKHQQIQKRHQNNIGKNKLVQESYLKGNFIFYKQENYFSPTLEQLIKVLLQSCIKKISYCTKKRLQIPLYSNVCNTPHEQVEFSQQTHTLQLLQINNIDDDEFQINFTVLGLNEKYASVYCSIEQNQVKGTVQISFQKIQVNLTIMSQDSDNIKICLDVLLSNILSTLNIKGQSINFFALLDPIKDKIDSQLQQSKIISLLVDSQQRMVLTEKLCKLFTKQLLTISTLSQRNQQQHNLDFYVMKKLLSVIGTNISVSVGKILYTYVNFIKSKGVCNLFIVQFVYYNNTQCIQILAFQQLVYLYIRLFLYAFYKIISLIIYIYISFAQNKGILIKQYQIIEIIFQLKYREIQYYILETSKILFAQNTGKFNIILQSLKQNFRLKYREIEYYIIEALKIGFAQNTGKLNQFFIIFNYVTEIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PS00</accession>
    <name>A0A8S1PS00_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1270064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PS00"/>
    <dbReference type="OMA" id="MEWLFLL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="456"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8105423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="793" mass="94417" checksum="64A57C923E2CDD0D" modified="2022-10-12" version="1">MSIVNASENLININSPDNSRATLVQKRCKIDADWKSTFMLQTIRNKAEKREVLMKCINRMRQSTQVKQFHTLTEKQLNVIDDFGSGHSKGIINKQIKKQETQFESTYFESKFQIGKLRGLVLRYIEKHNKNQEWSIKPSHPFIIIWNMFKLFFMVHIFILFPLIDAFGVKLEQMIVQQQQIFLMEWLFLLFDIVLRFNVQIYKNGQLIKQHQYLALQYLKSGFFLDALGIVGFSLFLFQDILYIKYIYFFKISEIKKFIKFLRYELDPQNKYNNHIKLITLFVTIFLLAHIIACLWIVAGHKEPSWLSNNDLQDDQWYIQYLWAFYFAILTMTTVGYGDIVPVNEIELMACIMIVLLSSAIFAYTLNTITTVLKDIDQNKSQFNQEARIIQNYLVKKGCDTDLQRRVQQYLKSLLKWGLGEQKQNEDRIFSKLSLPLQQEINLQDKGQVLLKMPFLVNNFNIECIKELMQHIQEVYYQPGELINPQDGVYLLYKGQAEVCWGSKTNPKHICNIFQGDHYGLLNLFNVDQPKYFLKSSDFSIFYFIPTQIFRQVLKNNDKEVFCMINDQVIYENYSNLYLYCPLCRREGHLICQDIIYKPNKAIAIAKSNRYTIQNRQKYKRFFVKQQILRNYKEMQIETCQFINDKREIMKIKYKSIFDSDDADEDPESLELQSSQSWQFDEAQMDEQQISNHIKPKITIQKRQTPNKRQTILGFLRKNTQDVMQINLQKIEEVDTPHNNNCTPNSKDKFYNLDVMKSFMKYFKQYNYDNIIKQINKKRKTMKTKIKRLPRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PVT8</accession>
    <name>A0A8S1PVT8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1330029</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PVT8"/>
    <dbReference type="OMA" id="SHNKRIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8107164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="36090" checksum="40F0C65B677514A5" modified="2022-10-12" version="1">MGNCESEQPIQSQQPVQIDQSSSHNKRIFDHKAVENDEFISNKVPAPLDVNGEIEDELEKLDPQPAIINLPPFNFGEKKHLNAESRPAYRLVDGSTYVGEWVGDQMTGNGVLEGKNGKYEGQFENGRKQGRGRFTFPNGEVYEGDWKDDQEDGIGTYYYSQGTVYHGQWVQNQKHGQGREVFPNRSNYQGNFENGIKTGHGVFIWKDGTKYEGRFVDNKICGQGRMEYPNGRVYEGEFLNGKMHGKGKFSWPDGRNYEGEYQYDKKHGIGILTGQNYRYEGEWKEGKMDGKGTLTKDGEIKVGLWHDNKFVSENHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PWX4</accession>
    <name>A0A8S1PWX4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1320106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8107019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PWX4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="77" mass="9090" checksum="AFDBC72B9439D10F" modified="2022-10-12" version="1">MDEDGKEVKLFISSTQIVDMFVKNLKKFFKRQYISESQYNTFSKSQHVISQFLIIGFGLNQVQPCYFATKMKYNGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYL4</accession>
    <name>A0A8S1PYL4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1340047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYL4"/>
    <dbReference type="OMA" id="IQSEYNF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="43057" checksum="366C1D1513B7213E" modified="2022-10-12" version="1">MMNVQQYQNYFPYVNEAQVLFQGDIEQAKQSQGSNYNEFNLFQQNFYPPTFPPYQFEFVQEHEIPSQIKNKKNKKHKNNDSKGLLPKTLESDKQFVEDQQQIIIQPDQIIKQPYPVFHYTQGSPLPKLQSDIVSNLHQDGQGSQEGSQQTQKQKGQHSQIKKPKKENINTGHWSTDEHTTYISFLQQYEDIMTSSMMKKTSKIFKQMSELIGTRTPSQCRSHHQKFNPYALRGENGKRLPRAERSRAGRKKKNPQTDLPKAEEANIIANYEAHDPYYYMMLEQQKYYYSNIQQEYWNPQHEMEPHPSIKKEEDNDFINNPHLQQLQQEYEECLKYNLPYIQRNGIQSEYNFEARNQVINDLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZM9</accession>
    <name>A0A8S1PZM9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1370111</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZM9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="153" mass="17381" checksum="3458781EB9ECDF5F" modified="2022-10-12" version="1">MFQAEKGANAIPGYTGFIPSQDYDQDLLQIQGNRNHIPNYAGFVPGIKSENLFGKTFGKITLLSTTHEHHRGSDLPADIRYKSEVKEAYCDQRDQRGRDINLYGKLDPETSKALAYTLSNLTHFEQTNTLPKRSDSKDRQSNLTYEEALQKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q3M5</accession>
    <name>A0A8S1Q3M5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1430086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLOC1S2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000147">
        <property type="protein sequence ID" value="CAD8110239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q3M5"/>
    <dbReference type="OMA" id="KMNETAH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019269">
        <property type="entry name" value="BLOC1_su2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10046">
        <property type="entry name" value="BLOC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="43"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8110239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="113" mass="13325" checksum="8826543E0B5BCE20" modified="2022-10-12" version="1">MIGKEQVDVIDQYAQSIINYVDNQQKIIQLKTTTIEKLNEVTIVHYQKNLDKINQIRQEFEETKNIQNKLDENIQKINQIQNSIEGMESSVDHLDSILKRIETKLTELEKLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7Q0</accession>
    <name>A0A8S1Q7Q0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1470042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7Q0"/>
    <dbReference type="OMA" id="QANQNVE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="239" mass="27305" checksum="01CC70599A837DAE" modified="2022-10-12" version="1">MNNHQVQSPQIFNSHYLRTDYKVNPQPLVLNLNQSRQSSKALSVDRVKLKQANQNVEQLIDLWQRHKSITQTTPTHISQSFHYHSTTQKNESFVIEEIPETREVSPLKPEEIMIKPRQLFEINQSTSKLPSTHQQNSNHKKTSSLYVGALSKSDAFSGTKKAAKENLKLSNNGTSGSKKTQFNSSSKKLPKELSAIVLQLQSVQKSLLPYYQKLDKGKKTEIDEQFNILINQIKSDFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q991</accession>
    <name>A0A8S1Q991_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1500076</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q991"/>
    <dbReference type="OMA" id="FRNENNF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000041">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="4">
        <location>
            <begin position="183"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8112182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1035" mass="119867" checksum="C50CD4F2D44AB44E" modified="2022-10-12" version="1">MIIIRALRNTFCRASISTSTTTSFPQVYRQKSQLLEVSESCKIINQLPHELIEQSCNIITNNFFPLQENYKRIIPKIEEVLKNPQLIKGTIFQGQDSQSIAEQIYEMVILIVDFADKLNIGFLTGDIHPLTFNIIQREIEITQDVDLLNENLRKSLFNIGKQYKLPLNGIKHPDNENPRNLFERQLIIEQVSNELALDKFQQVFDDLQRMSKAQHLFAAQKYIVEWFPKLCALVQQEQIQCFSDPNKEQQGYHQYITAIQSEKLAIIALSELMKEFIEAAVLFHEEKTTLNCYQIRVTRLANRIGEQIMKQHLYEEEQKNLRRKSKEDIQKMIEEGTIPQDQSEEEIQNQKELFYKQAVVKAGKLLKTQEEKLRERIRNNRIYLAKYGQAMPKDIASQIGMVLVHFLVDSIKFRNENNFWVPVLVQGYKKVKQDIEVAIFNVNQLFLHHLSAGMHKDNSYFMHLDRALPMIYPPAKWMDTQIGGYYLKPTNLIRIQDQSLQEDAAKRANLQNLYDILDNISKVAWRVNNKVLNIAMQVWEQGGGIAEVPKRQHDKTYVFEHQIKECKSYEEKYQLLKKIQSQRDLYSLQCDFTLKLGVALAFNGCSKIYFPHNMDFRGRLYPIPPHLNHMGPDIARGLLEFSEGKKLGKSGLRWLKIHLANKMGKDKLSMADREDYVDQNIDSIIKCAEDPIKYQDWAQLEDAWQSLAAMFDYVAAIKSPNPEEYVSHLHVHQDGSCNGLQHYAALGRDVEGATQVNLANTSKPGDVYTHVAGMVERRVEFDAKDPLSKDHIIALKLQNQIKRKIVKQTVMTSVYGVTFIGAREQIHRQLKDKGIIGDDDHLQYQASIYLARITLDAIKDLFEGAHKIKQWLIKCATIISNENQPVSWITPLGLPVIQPYRSLNKYDEVKTLISNVSIEADAANLPINKVKQRSAFPPNFIHSLDSTHLMYSSIECLKQGITFAAVHDSYWTHACHVDKLNELLRDQFVRLHSQPLLEDLRDSFQRRFPHLEFPPIPERGQFDLEKVKQSVYFFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9R1</accession>
    <name>A0A8S1Q9R1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1510103</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000156">
        <property type="protein sequence ID" value="CAD8112476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9R1"/>
    <dbReference type="OMA" id="QKMDHAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="34"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="70"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8112476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="361" mass="43681" checksum="C08EA425CCE4CD3C" modified="2022-10-12" version="1">MNYNLKTFDTIKWLRNRYKSRVKDQYILYESELKEQNMMKAVFNSIDRDKSKFLDRSELYDMFIKYGINITKSKLKEFFKSIDEDEDDKLNWTDFKQALQNQEALNMFVQLMKKIREDYEINQINPTQQLTFVPLSFPQMIQYMNYCVLREEIINKIESDNINNYQKHKQCVNLLTLEDNCYRNIKSDQIQDEDEENETKNVQKMDHAQLLQMKRFQERELKFKRLEKVKDISKRKFYTNPTQDLNVTDTLRLSDITQLRTNLTQEITRTRDNIDKFLAILNNEKLKQQKSRQTSRVSSRQKSREKKTNIFKKNTVLIEAEKELLNASAPEQYKFKELNYGSHHKKKYPRERILSLGMKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QC19</accession>
    <name>A0A8S1QC19_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone-binding protein RBBP4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1540064</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051972">
        <property type="entry name" value="Glutamate-rich_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903:SF1">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="4">
        <location>
            <begin position="126"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="32"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8113158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="419" mass="49547" checksum="F78C349A7405348F" modified="2022-10-12" version="1">MQSRSQRRRRILKQCIQKKSKIEDNAHVTQEIDSEYEDVSEMEEDEHIQYEDEFDDEYESEEGEINIDSEDEEILEKEEQSQEQSQQTQQQQQPIQQQSKLKRKKKGMEQENQENQKRVWFYDEAENLDFDNRAYNMLHRVTTEWPCLSCDFVLTEEEQQQYKNKEYQKMNKYPYSVYLAAGTQAAQPTKNQIYLLKLSKMHKTKYDDDEASQSEEDSEDDNLSNDEEGQVHLSSVTGLKCGVNRIKTMNGQAIAAYWNENGDVNILDLNPLYKKLQSNQQSQFNLSQLHHKVFKNQHEGFALDWSRLKVGDLLSGSVDGKIYLYQLNNNDWIRENKAYEYHKGSVEDLQFSPIESFVFASCSTDGTLCIVDTREGKHKQAQXKIDIVNLEYYNKLTQQYKDFYVNLLYLYLINKSWNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QC52</accession>
    <name>A0A8S1QC52_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1540079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QC52"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035890243" evidence="2">
        <location>
            <begin position="22"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8113188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="349" mass="39613" checksum="B6A9DC85F7BB96FC" modified="2022-10-12" version="1">MGKSMSWISAVIASAFCWASADTAFDVILDSQESDLPQKKQQLPLNIHLTHSQTMLLCALTTYIIIFVYFIYIELIGPQYSELIGNYSIFGSIQSLPLLIISGIFSYYHYHFIIRAFENSSSTVILPFLQFSSIFAFLFSILQNYLSNRPYLSSYLHLLAYFLILLGGLAPATDGHLIKIFQYQFWSQPFVKFAIISDMSHAIYTNIISYVGKGENEGQQYNIYGNMEFFILTRIMFVIIQTAKLNFNRQMQLEMIQLFNKPKNLIIGTIIGEIMTLFGYFSSVYAYQTYYQAGVVSASESSLNLIINLFLAYLLKKYLNIGKQASLHNLGLKIVSCVTISIGLTIATI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDH9</accession>
    <name>A0A8S1QDH9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipase A-2-activating protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1550087</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDH9"/>
    <dbReference type="OMA" id="DKCIYYW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015155">
        <property type="entry name" value="PFU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013535">
        <property type="entry name" value="PUL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF0">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09070">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08324">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51394">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51396">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="93"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="212"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="PFU" evidence="5">
        <location>
            <begin position="349"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="PUL" evidence="6">
        <location>
            <begin position="477"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8113493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="748" mass="85070" checksum="65BD31C2E938B54E" modified="2022-10-12" version="1">MEALQYKLSQTIAAHNGIVRSISTQGNELLTCSSDKTAKLYEMKDNQYQQVTLISFFEKYIYATCARVNGGYAVGHDNQIYLLDNEGNPLGILEGHDQQVCSLKSISENLLISGSWDATAIIWDISQMKQIYRLNGHKYGVAVYGDENLNFITGSQDGILHSWSKETKIKSVQAHKDIIREILLSPLGGYITCSNDETIKLWSKDLELIQTFLGHKSFVFTMKIHMDQVISGGDDRLVIIWNLDGTPQQTIQLPDTVWTVAINNFNDIIIGTSDGKVRVFTTDKNRFATQAEIEGLEQEASLSNAKQEGGMSEEEVQKLPGIDKLNTMIGKKEGEIRLFRNGNKPEAYMWSAASSNWQLIGDVIGGKGANSKKFFPGDKYFEAGEYDHVFDVEDDHGITKLLPYNEGESFYDSAEKFCLREGYSKHYLQQIVNFLKKNTSFGQSQRQKKSELETMKEQYAQQQILQQQQAKQQIDFQYIPYTQCTYYENMNLQGLSKKLFEFNAQMSEELKLTEKETLIFNKGIESLGQVQVQKTVNIENSVSIIFIQKLLKWDAQYLLPVYDFFRLFCLHHSSEQLFAGLEKGMNLFLNIFTIVNTQPINAVLVRLALQTLCNCLKHNTNSCAILYHLRIVKDIILSLLDTDEKTVLLLSNLILNLSIGIYQRNGLNDQASELLSEAIITFLQYKQRDVETIAKLVTAIGNLMKSPAKQIREQCKKITFEFIQSLVIDTNNQDTLKCLEEVKLSMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF07</accession>
    <name>A0A8S1QF07_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1550019</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF07"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8113357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="93" mass="10704" checksum="B15A2B7C7CC0B40A" modified="2022-10-12" version="1">MVQLASNRSMMVKAINSTWSIIQTLYFAIILFFFTLFGMKKESNKYSKEELKYIINEKMPIKRKPKDDEDDNDKEGTKPKIVRGFGGGADCKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QMM8</accession>
    <name>A0A8S1QMM8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1710021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000180">
        <property type="protein sequence ID" value="CAD8116331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QMM8"/>
    <dbReference type="OMA" id="CVSSNRN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="132" mass="15179" checksum="83EFA4ACC778653D" modified="2022-10-12" version="1">MDQLALKKLVNKLIDEKLKLVCEECRKKTTQKKLGYHNFNISIQPSPINFDFSIDNENKENCLSSNRNANRVKASKSQALDLEQQIFNADISVIRPISQFSSPTVKASRKQSMPFLKKETQTTYEKVLKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QMX1</accession>
    <name>A0A8S1QMX1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1850003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000194">
        <property type="protein sequence ID" value="CAD8116949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QMX1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="75" mass="8739" checksum="9694133DD5B7C1A9" modified="2022-10-12" version="1">MSIECSFECITQSCNQYNGQSDCNYIYTSYTLREIALCYWDSLYDECISASISLAKRLIPLTQHNVMQIQDMYIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPK5</accession>
    <name>A0A8S1QPK5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1790003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000188">
        <property type="protein sequence ID" value="CAD8116735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPK5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="9041" checksum="E0D154B84E29D91D" modified="2022-10-12" version="1">MIYFTQDHCLWMYYFIIPGLIVIDVMIPLKIVVLSMINQFRIDKIKNQKVLLNEQEMDQIIQESNLNIYSDQFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QR31</accession>
    <name>A0A8S1QR31_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T2200002</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000229">
        <property type="protein sequence ID" value="CAD8118011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="567"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="665"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8118011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1049" mass="120964" checksum="67548052188CBDA7" modified="2022-10-12" version="1">MCYFEMSQNCKVSFLNICQICQENFQLNNNNLCIPECGNGIVQEYEFCDDANIVQFDGCYQCQSSCQLECLQCSYSQCFQCIEGWNLIDFKCVSTCGDGLIALLQQEQCDDQNDESNDGCFECKLECGQNCQFCNNKLDCVQCEKYFEIKNYICVPICGDGIVVEGFEQCDDGNNIQYDGCYECQFSCKDECQICNKDKCLDNNQTNLCEIGYQLIYNQCHSICGDSIITSNEQCDDANEIPFDGCYQCQFSCSQQCLDCIDGQCLKCELDYEIQNNICIDVCGNGSKSELEECDDNNQVSQDGCSDKCEIEINWQCTQINYKTSQCLQMKPPKLNLIYLNQTNDSQFIQFQITSKVKLQNSEQNFTDNLKFSLIDMNPQDYIIYEYVLVEPNDTTLKDINYLFQIQLLKQETSEIYFQVQFDALLIDEYGFQVENQFFKLHLKNTIVLSESQRIISNKICTLNIYILIGLGLSSFIILLSGNPGDCFEVLDTMQYQSNLKYINSNFPENVMIYFESSQVVTVIPILEKLKILDLLDTTIGSEQIKSFGKFLLYDVNSDLITNLSGLIVQIGLTLVFLVTSKIYLFLFFKKYYNKFRIFIYKNSKFFLSSKVIQFIHKLNKLGMTINQILSFKGLVYVIQANSWDLLFKTLIYLYSKKENNLRNYIQNILAYSFITCILILISQIFSQKDSQIDIMKRKSLKHEGVIVAKKLLFLIVLIGSQEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVYNVKSILKLKIIYVFLFVEMEQWQKDLNNVMTEITFNMMVVMNVNFPAKMNVKFAIKINVQIITRLIYVKQDINQFIINVIQFVEIQQLHQMNNVMMLMKYLLMDAINVNFLVHNSAQIVQMDNVQNVNLIMKFRIIFVQMFVAMVLNQNQKNAMIIIKYLKMDVLINVKLKLIGNAHKQIIRLVNVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JLW9</accession>
    <name>A0A8S1JLW9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JLW9"/>
    <dbReference type="OMA" id="ICSLEIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8042964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="152" mass="17897" checksum="E071E817274D7772" modified="2022-10-12" version="1">MNKVNTKSKQQVQNDFDSFQSDNSYQSDGDNQPIIMNFLEDIKGHLDGDISFETNEHDIYDPDFSQNISHRIKEHQETAFQKQKFKSNTQSIKSCNSINIICSLEIFQQQLELKHTQSLPDQQNLQTISDSKKQQLNFLWEKIRQKNKDKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JM17</accession>
    <name>A0A8S1JM17_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0040405</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JM17"/>
    <dbReference type="OMA" id="EMFGGCA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF56">
        <property type="entry name" value="MAPKK KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="55"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8043182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="362" mass="42223" checksum="29AC5485ADF8CAD8" modified="2022-10-12" version="1">MGYCGSKLSRKDKQNNKNNINDSQFQQYQIQNILSQSSNLNYSTQEQILQKKRDYKKSELIGKGSLFEVYQALDNKTGKIMAIKTVKIQGTKEQKYQLIDQLKAEIKLLKRLKHKNIIKYYFTEISSDFTQLDIALEYISQGSLKRVLSKIDLEESNIRIYSKQILEGIQYLHKKNIIHRNIKTSTILVDSEGTIKLSNFKNFIQFDQDFDINEKQKSFAIDILSLGAVIIEMFGGYILNFDELIKNISEVQKPPYPQQASALSKNLLDIIFVPDHQQYSTDKLLQHPFFNFEQEKKEFQLDDPNSDQSLIGSSFEVKKPFIQDINQDQSIQKCNILQNNKQRQQELEQALQSLLRQKNQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN04</accession>
    <name>A0A8S1JN04_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0050347</name>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0140178</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JN04"/>
    <dbReference type="OMA" id="GRICDLQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48038:SF2">
        <property type="entry name" value="OS02G0536400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48038">
        <property type="entry name" value="RIBONUCLEOPROTEIN RB97D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="11"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8043693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8049815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="183" mass="22228" checksum="969FFC1C43DC691D" modified="2022-10-12" version="1">MSRQQFRNARMSLFVGNISRLVEQKDIEREFKVYGDCTVDFRVKNNDRYAFIQYKSEQEAEAAKNALHQRDLQGLRINVEWSKKSGRYDENREPRRDRPRDDRGQSRGNYDRFEREREKEKKQRRRNSKSRSRSRSPKNRSSPQYRVSRSQSIDSENEPPRDKILRKKLKEMMRKTDNGEYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNS9</accession>
    <name>A0A8S1JNS9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0050335</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the USE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNS9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019150">
        <property type="entry name" value="Vesicle_transport_protein_Use1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09753">
        <property type="entry name" value="Use1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8043681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="25542" checksum="1288E0FC35B89D32" modified="2022-10-12" version="1">MNNYTALSGGSQFQDIIEEIKKVDAIIAQGINQRNQRLFQKESVQLIDRQLGQQINNINELIKQGRTQLYKMKNLSQNEQQRQKNKLDEFQNRSNEFSRALQVKQIFNMVTTQTNKEYGEKSDTEMVQIQKTLKKEQDKILDNMLQTTDIIANNTKTINLALKEDKVILINLNNNVEQATNEIQAVDSKLKTLLSYTNDWCLWIIILIEFVVFIFLVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPJ0</accession>
    <name>A0A8S1JPJ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0060487</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPJ0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="120" mass="14272" checksum="FF183C492DD731CE" modified="2022-10-12" version="1">MNQLIKQCLEREYQRQGLSDQPHSISVNVVVNLEKANLKLSMSIDPEIPFNIFLQGIKEQIFAQRIMVIMQWKQSSTRRWKNSLIIRFYKQLYIICQIETQRWTKKNILLSISGFAIQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRQ4</accession>
    <name>A0A8S1JRQ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRQ4"/>
    <dbReference type="OMA" id="YQNDEIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="38664" checksum="5CE932C2595CA950" modified="2022-10-12" version="1">MQQLQNCLLDKEMILQSDGTLYIGSFYQQHFEGYSLVINPDGSYYYGLFKKSKPKEVGVYKLKNSCLKIFWGDDNQIKNVKDIGNIGFLQKIISFKNNCYGNKIFIKINPLSYGQAKNQKYDGFTVMHYKNGDIYYGNIYKGKQDGEGIYYNNNIQEWQWNKYSNGKLISHQLYGKNLVQQLIINQLLINNQQNHLSLASTVQSSHPAHRENLSQILIDDVDNSNNEILEILQSSQIESIDQELHIYNQNNQKDEIEIKLSKCQKQKAQQRIEKQRQNKFLTQPSQTKSPKNSRQQQMTLSTMQSTQKTTIYKRSVSPIFTTVRHLFSVIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSH0</accession>
    <name>A0A8S1JSH0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SF-assemblin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSH0"/>
    <dbReference type="OMA" id="NIQMSRV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="34"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="29667" checksum="356EF3CFA7A33F2A" modified="2022-10-12" version="1">MSRVKQSLYAQQSPNTGRIQKISEKLSTIQIGVENERFQKLEQAEQRIQQAEDAFNEFQEQIFTKLNGLRDQLGKLYKQVDDDKMAKEQAIEAKNRDVQALTKKFENAIENEQQTKKEGEAKILRLTEDKSALLRTEVQKETAQRVDAIEGIHQGLQNDLPKIQEAIREEANERDESDQNVMKSITDELVKLSNLINVEKRNRDESEQSIFEMLKDIVNRVKVELDQEKRTREQSEEHLLSLLEDTCNKLSIAANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JT61</accession>
    <name>A0A8S1JT61_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JT61"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="206" mass="24677" checksum="AA340E5F5938FC7E" modified="2022-10-12" version="1">MTRSYSCHKRRLSEISNRQNNNDKNYIKIIESSRRNKFLDSKRKDIIKQQEIISENKRLLNKIVNIKSSFYCQSSRQSISSDCSKLSARSNEQKQMNVQRIKNNYLIKQKRFQIKATFQTIPIERTSRDQLRKPIYQIESIKQKQKCLQVQKINQLRLLSLSTLRNNNRVRISNSENIRIVNTLPEIKDKNYQIIARRLKDKLYNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTJ7</accession>
    <name>A0A8S1JTJ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTJ7"/>
    <dbReference type="OMA" id="QQYFITP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052976">
        <property type="entry name" value="Scoloptoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22933:SF31">
        <property type="entry name" value="FI18007P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22933">
        <property type="entry name" value="FI18007P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="385"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="428" mass="49666" checksum="528507ACB1086D3B" modified="2022-10-12" version="1">MNNAAEKLSDIFNAPLTELESNIIESAIKYISQPQRLLDDLTQSIADFKAKFYESLHNLLQFFGQFNNDLDIIKATQQIEIHLTKYKQQQIFIQMKNILNLKLKALLETFCVDNNQKLEVSNQIKSLKDSLMKIESTLLEQKQILSPSQQYFITPHPKKGFIQTSSKNNLTPNLSDEAEMRGKGVFITPENRQQQPRQFEHSHQQYSSTDSYSVVQNKTLNSFDKEKSENKTDLLSPMFGFQEQNQQSTQFFQDDIINKLIYQNYLEQNNVDIQVDNHQQQQTTNILQQFQQTFQKIDSLQTTRTEQLDNPLESENQQKIRNQSVEPNIRDNSQSPNGKRLIQTSNSKESFSRCKSTLDTTNLLQRLNQVEVPKKINKMSKSNKENQSQQNSKQLKSNKPLSFQKQKGLLITSKTQSQQNLIKKSKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JV23</accession>
    <name>A0A8S1JV23_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JV23"/>
    <dbReference type="OMA" id="GYITNKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="323" mass="37914" checksum="081A7B74BD66A48D" modified="2022-10-12" version="1">MLTGKEELDKINPKSAKGNAESEELPQYLRSSYSQTYQILEPTKILKGKKVFPQFAYGNSHEAPTYFKLSNSLVCKESYDNPYIKNKVPPLSKLDSLKGSTLNNYEFYNISDQRFTNRKPTGYITNKITDTITNQVKFVQKPLNDDKDQMFTFESAYKSIQDKLFNKKRELFTDFVEKNDLLNVYGPLNMRTRPKEFREVKNYALDSDQLKLLKEQRSRQQITPTNRSGFTDLIKTVRLPTISKNQGLDTDKYYNKDQHYLNQLSNSIIVEKIDENIKHKNKTLQSFNKNKRSIKHNTEDTENMQGIIDIFEKHLEFEKQYNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVV1</accession>
    <name>A0A8S1JVV1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVV1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000157">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000101">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="410"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="809" mass="96159" checksum="244A870E8DE1BC49" modified="2022-10-12" version="1">MAICSINASGSEYIVIGVERNRTLKTLMVRDNRLTDKGAECIFKGLFFDKIDLKRCNLTDKCTLGLCNMIKQSTQLTTLLLPDNYLIMVFMILQEFLYVKLLELILQYICENPLSYKYIDQINKVVETNRLKFEIDRVPMYKREIVKLNNIIQMKPSLEKGLNENQQKKARSLQTLEKAIEEKNKVKIEQIQKTSDMDIRLQQIIKIKIMDIDKIKFDNESNMYLIKVDDEINDYQKKMSESHQKKKQGVQRIKDMFVKVKSDSRLEMLRQLNRAEERALQRADMEKKTTGRKDKGYIRKRIHYQQKENQVKVLNYLIKIMNKKYTSSNLNPKVLDDRDEINFGTSFIDAILDQKLFEYLTAFLDTLSLIHFQLSSKVFKIRHWKNYFKKVMFNRLLKPLDHKLISQFIIDQKCVQFIFWDSVIDFKRLNLQYSHQYKYYSCQQTVEASNIQKDIDRTFSQHQYFKQIHNRQRLQRILIALSKIQEQIGYIQGFNQIAGCFLINGLNEQQTFWIMYYILKKMKYSTIFQDQFNELNFLNFTVAVFLRNYVPSLSNEFLQNKIDIGIITTRWFLVIFGYDLPQQLLIQIWNMFLLKGIKILIRASIAIFKLISDFEGINDLYELLKDNLYDLLETNVQLQQKFIEYFSKIKITNRLIEELREKFETGDESLSLSFDQIQKKHYWRKGGDSARSLISSFNEIISEIQEEKDACFQRSQLFMNAFFPKLINQLVASEGKKNLAIKFEKRPQLIKRQIISQQFKMSIPQSANGDQESNIYADELQSRMSPKEDESIELECTPEKIFKFSNKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXI3</accession>
    <name>A0A8S1JXI3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Activator of Hsp90 ATPase homologue 1/2-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXI3"/>
    <dbReference type="OMA" id="ITQTNFP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08892">
        <property type="entry name" value="SRPBCC_Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="ASHA1/2-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase homologue 1/2-like C-terminal" evidence="1">
        <location>
            <begin position="12"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8042918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="133" mass="15651" checksum="313ECB86333AA89D" modified="2022-10-12" version="1">MDKLEFSIVWGVPSKVIYQALLDPFEIMQYTRAPAVVEAKEGGLYKIMEGRIEGVFNKLVENQEIQMTWKFNNWKQFSNVTLRLIEREDSCELKITQTNFPQDIDKVKLEDGWKNQIFIPMSKIRGYPIEDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K006</accession>
    <name>A0A8S1K006_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cation-transporting P-type ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0120440</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K006"/>
    <dbReference type="OMA" id="GINTYHM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="742"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="766"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="805"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="846"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="885"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="919"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="6">
        <location>
            <begin position="143"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="7">
        <location>
            <begin position="759"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8048381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="992" mass="116558" checksum="D692114E943E35D5" modified="2022-10-12" version="1">MDKQPLLHKFTITPNELFQIISSQNTQYTATSLSKMLDMDLQIGLDSSNVLDINKRKKQFGTHKIYDSQTIRYQLVIQIIKNKWHQIFFAITLIKLGLSMLITNSKNERMESLFILVFSLLQLLIRIFKTDNQELLNNLRQFQNNKVKLKRNDKVINISSEQIVVGDILIFCEDQLIEVDGILISNQTNQTILFDTNTNKLPFIRSGSKVLQGNGQLLVLAVGINTYHMRKCDLNLQHEYTTPLQERLNYLQQQLIKLFFVLAIIPLIIQFLNYCYHISTQEYLFDFVDSIHSILKQCQISILLLLMVMNDNLSKFVSTYLQYNQKRMKRDRIKFQRPSSCEILGRINNICTSKSQILTNGNTRLVQIFAEEQLLMKFDIKKLRYSTIQLIQENICLNSTANPKISYRSNDITVFEKFGNEIDCALLEYCHELGLDYTTIRKSYQNQIVQYFQFNSDRKMMSVLIKLQNKYILYTKGAPEIILEYCTDFINSKGEIQKLTEQSKQQIYNQIKKFGEESYRPLCFAYKNIDININLKLLSEKQLEKQLIFVALIALEDEIRQGVQRSIQQCKEAGVIVRMITNESLECSISLSKKCGILPMNYQHQKDSRKVMTGKTFNDLVKGITYKKQDGQIIPKIGDMDSFAQIAAELRVLVRASLDEKLAIIQGLKELNYVVGVFSHSNEPAFYRLADVGFCQNSKIIQQYNYMISVSILDDNFSSVIYSIDWGRNIFDAVRKFIQFKIALLLSLSILQTFSIFHYFNQTQLLWITFIVDFTAFFMYATEIPSRRLYERQQNERYHLITSNMWRNILSQIIYQLLVLFMIQVATQKYIEMKNSQNCYHFDLKSHTLLFNTFVYLQIFNLMISHKIRKTDICISSNKREKAKLIIQLLLIFVMQYLVVEHCNFIFNLSKLTMKEYFGTLLLSSFGCVVTYLFKFINDKYFNWIMLFRSQDQRNHYSSFDEFLQFKSILRQIKSYPTEKEDNSEIELTEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6P7</accession>
    <name>A0A8S1K6P7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0170059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6P7"/>
    <dbReference type="OMA" id="RRSWSKK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="160" mass="19019" checksum="65C0BC48EE5ACAEA" modified="2022-10-12" version="1">MGSVCKNMKNTQDLKQIQMNCKNNKTFKKLKRADFQHPKQILDFDSSYSICSERNSSQFSKTIGHINTSSCIYMKRRDNQFFTRRVNSSLNRNSLNLLKKDDYNSQSDLSIELIDDAQLKLIIPYHARRSWSKKRVTTKVDLLKKVKNQKYLERFKRRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KC49</accession>
    <name>A0A8S1KC49_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KC49"/>
    <dbReference type="OMA" id="DNRRINS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="453" mass="54617" checksum="F2362C3B0839F4D1" modified="2022-10-12" version="1">MNYLLLSDQLDYQYDCLELENDQWTSISILKGFPQYIKIRIQDYYPLKIFFNVGAFKCNIYFSFQTSQPSEKNHQKHVVIKQQKIIVIKRPEEQKNYLYFSLLSDYSTSIKLKAWFQSSQYDRCYFLCYDGKEQFRMRSGFQNNNNTDEESINTNKNNITSVNIKSAERSLINESVPPIKSFSDNRRINSVKHRNEQISLKNKILLVQQKKLELLSQNVIKKYQKMLVQSYKQLNQSRMMKKYINQWIINLNVLKFIEMLYAHFKINKILRCMNNSPKYYTQRWKQMNEKTSSLNMRVLMQANLTLSIFSKQLNEKISKQISELILITLNDTLFIKKQAQLQQSFMNFYLKVKQIQEFYFTQRNKFYLQMEQMIQNIRQQSPQILISKQRMKRIMFEFYQQLCTKHKQEYQRFLELEVSNVTLLTSVFAIFSKPKLNIMKNQILLLQIIEQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDB3</accession>
    <name>A0A8S1KDB3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0200229</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0200231</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDB3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8053243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="95" mass="10891" checksum="1E112923909209D4" modified="2022-10-12" version="1">MGCNSSKSNGRQLNQSENIKRNSKQSVKLQEIQQNILKHLNQQQCQTRTSVTSLKNPLSSYKNSRINDQKSELVLSTIMPQTDIGTQLLRKKLEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDR1</accession>
    <name>A0A8S1KDR1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0190179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDR1"/>
    <dbReference type="OMA" id="GRWKYCC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="267" mass="30023" checksum="3343876984922376" modified="2022-10-12" version="1">MNSNNNGLNDLELLRLIQQEFQQEKSPVYDEISDQYSSVSDDEEIIIRDPSSQITDESSLSQQQEKKKGRWKYCCQQCTKSDKGSIKPKTCVCIVPASQRRIQIGEQGCLSCHCTGCSIEDEKKKQKKIKKKRKSSSDSSSNESSDFKTVNGCCKKCMKAFSKKGKEAKSCLCQVPRAVRKKPLPPHGCQYCGCHGCNPEDKKKDKPKKNSRQLSPVSSDLNEQNLNNFQNAPLIQLLNSQNINPGIIGIGIPQRTYSYIYGKEQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHN0</accession>
    <name>A0A8S1KHN0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0220364</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8055020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHN0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="218" mass="26300" checksum="EAC9F0C98B9C38A2" modified="2022-10-12" version="1">MPINQTEALYQYQYGLDELQLKECKEKLEKKEFLFLLVMDNYDEMKKNLYLNNKLKQNWQNLIIIFTKSREIDKQKLFCVVQTGRQEYIKRNQTSKIEFSINRNIQKIFQFRVLKFIYKKRNIIDRGKSVELKINKLYSSFFLEQQVYIFKSHDALRSLSLNLQKLWSVKKYDKMIRQINLNKLMVTPFMMDIVVQVVALNINLSVTIEKTFGIKQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIC8</accession>
    <name>A0A8S1KIC8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T0220273</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KIC8"/>
    <dbReference type="OMA" id="CEVWIVM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="101"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8054919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="320" mass="36733" checksum="296FD0E2A6D52D67" modified="2022-10-12" version="1">MGNCIFKFQVNTQAQNSIQQGNEIIDQAINADDQEQQTSIQAKIIFKQTSGVNLESIDSQTPNVFSSQSSNIKTKYQKDLSLSPDLFIRKQKEGEKFLQHYEIIKKLGQGGFGEVYQVRHLKTNLIRAVKIISRQSVENETLLLQETEILKNLDHPNIVKILEVFNDNQHFYLITESLDGGELIDRVRKIKNYSEEIAKIYMKQILSAMIYCHERKIVHRDLKPENILFDTLDINSNLKVIDFGASEKMISRKLTTKIGTPYFIAPEILRSNGYNEKVDVWSCGVILYILLIGKAPFRGKNQYETLQLVQQAQIDFNGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJG2</accession>
    <name>A0A8S1KJG2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0220242</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0220243</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJG2"/>
    <dbReference type="OMA" id="INIPYHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364:SF27">
        <property type="entry name" value="SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="THIOSULFATE SULFERTANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="26"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="178"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8054857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8054859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="295" mass="33626" checksum="BDFD3B7784666D68" modified="2022-10-12" version="1">MFIRSARYAFSSFPSPLVSVQYLKQNLNKVKVLDCSWYLPQMNRNAELEYKKSHIPGAIRFDIDANSLQETSLPHMLPKKEDFEKSVSDMGISNNDQIVVYDGLNIFTSARVYWQFKYFGHKDISVLDGGFPAWIKENCAISDAPPQIKKTKYKATPQPHMLRELDFILKNIENQNKGQKGDYVLDARPTTSFNGEVPELNPALPNGHMPYSTSLPFIQLIDQKTGLLKTAEEIKDILNSLNIDLNRNIICSCYQGVTAAIIYIALERIGLKNISLYDGSWIEYAFTKDVEIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KNL3</accession>
    <name>A0A8S1KNL3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0250147</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KNL3"/>
    <dbReference type="OMA" id="HINIRGD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22749">
        <property type="entry name" value="Otubain_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019400">
        <property type="entry name" value="Peptidase_C65_otubain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931:SF15">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTUBAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931">
        <property type="entry name" value="UBIQUITIN THIOLESTERASE PROTEIN OTUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10275">
        <property type="entry name" value="Peptidase_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="OTU" evidence="4">
        <location>
            <begin position="299"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="49"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8056970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="490" mass="58901" checksum="3CB178A48331B9EC" modified="2022-10-12" version="1">MQEQTSQQNHGNSNKKGDKLINLIIFIIKLFFQIIYAICNIPFKKKQGQEHSQIQEHQKKSNESNENNGRNKHLNDNKENQEETINQNNLNSNKENFINQQKQQKQEEQEQQKEQQKEQEQEQQQQQQQQQEQQQQQELQLIIEQTQQQEQQQQQQQQQQQQQKQEEQEQEQQQENQIIIEQQQEQNQQQQEIKNLNVEHQLDDLNQNISDQQKEIPQIEQQSENLEKSDQIKYEYLTQETLDLLNEIANKSYKARNVEIQFQKFIGQVNNIQDTIKHYFGRSQSKFSKQIKQMKKEIIGHINIRGDGNCFYTSFIYQYLSLLLNDEKRKIQFINQIEELSANIECLNIKINQNKDIVKEFSWQFQQIKTKQQLLNQISNPDYLFYLLSVIVFRNYFAHIFQQSEEYAQFGDLNIFADLMIWEQECNNNEILIAALIENLKLHLILYFIDIKNNSYEKKEYKPKQQIEGIKIEKIFMLLSPGHYQIAIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KNM8</accession>
    <name>A0A8S1KNM8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0250167</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KNM8"/>
    <dbReference type="OMA" id="LNTQNML"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="14"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="167"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8056990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="27424" checksum="41719DA49150887E" modified="2022-10-12" version="1">MASIEQLSKVEDLQQILQEDSSTPSHESDSSDSSYDFAQDLNQKPSSKRSPEEEKELLYKYRGKHGLKYAKISNEQRQKLIKQVTTTGCTIKSAAKELNINFSTAKAIMQIYRKEGRTSKKIKRENKKTLTSKQEILNQTTSDKPNYGEQRQTKVDVVQDNLETNLYQQAEETNRNQALLIQQLNTQNMLLQGRVQQLQQEKQQLTQNYSYLTYQYNQLQQMMSQMVPSYYRPYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KP82</accession>
    <name>A0A8S1KP82_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0250247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KP82"/>
    <dbReference type="OMA" id="IDSICML"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="311" mass="37862" checksum="B908FDC81EB51704" modified="2022-10-12" version="1">MSQVFQKIKRQCTFGTKRAEKCIEKDVQDKQISQFCNMDNQLALMNKEYIYVYNTDNGYQCYMLRQKLFTKYSKIIVYHFRFIKFQNVHLLLQVCTDRIVVFYKSRLIFKQKINEPLYRYDNFYYDKYENYFIIQGRTMADNFSLFIVSKLFSKNANRYCLTPNYLRLALDYNSIFYETKTIFKQLSQNYLTIDSICMLPQHKLFLYNKQEKELYLCDLNKLYQAYPTFAFLDEYEHSIFKIDKSQNCLPFQYIIGVYSKVYADKIEYHRNYLLLVQEKDPISEIVWKEQYCLEDEGTLSYMTKNGKRYRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSB2</accession>
    <name>A0A8S1KSB2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240314</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSB2"/>
    <dbReference type="OMA" id="THKIFHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035755176" evidence="1">
        <location>
            <begin position="16"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="83" mass="9633" checksum="9573D2B3AB370F89" modified="2022-10-12" version="1">MASSILNLLAGMGVGFYLTYNVDDETHKIFHKEIYVPVKNEMKKDDEPNYWTRVFNATQKGTQVSLTYLKNKQTKDTQNNKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTB1</accession>
    <name>A0A8S1KTB1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0280014</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTB1"/>
    <dbReference type="OMA" id="VEWWEAP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24162">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013881">
        <property type="entry name" value="Pre-mRNA_splic_Prp3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027104">
        <property type="entry name" value="Prp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010541">
        <property type="entry name" value="Prp3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212">
        <property type="entry name" value="U4/U6-ASSOCIATED RNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08572">
        <property type="entry name" value="PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06544">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor 3" evidence="8">
        <location>
            <begin position="160"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Small nuclear ribonucleoprotein Prp3 C-terminal" evidence="7">
        <location>
            <begin position="449"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8058780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="569" mass="66020" checksum="1C8A036A2DB17B99" modified="2022-10-12" version="1">MSKNKGVSKDVQTPQFLEKKPQNVQTQNLNSRLKQPDLNRKLLTMPIDAKYKAVLGPDTDSLVAYLKHKKKKEKAIQKLKESGKIQIVKEPKLSIDKSLPQGIYETEEGKLKDISGKIIRLNGQAKSLKINQEKQKHEKVQEMLKIQNNLKDALNDKGRFLDKNVTIQSSNIKRQKRKLLAFNFIENGNKDVEQVQQQTSIQIENQNEEKVQEKDIQNLTTTITTTNQTNFKRVCVKRKHHDPIPNIEWWDIPLFPENQANYIPINLENEQEHKQPATAHTQEEIQRNITFLEQLQYTDTNILLDKITKLLEHPKPFVISELVQNREKAALPSFLTKKERKKRLRKLRMDIQKEKQDKIRLGLQKPSPPRITLQNMMAILGAEAQIDPSKAEQEVRRQIQARLDKHIKQNEERKLTKEQRGDKLKLKWQKDSAQEIRVAVFRIDDDLSSTNLESKKLKFKVDMNAQQVSLQGVCLIASQNGQQLVPSIVVAEGGPKGIKFYKKLLLNRIKWRCNDQKSGCTLVWEGVSKQHAFNKWKTIEIKNEAEGKRILAEKGVEQFWDLAMNNHQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUH7</accession>
    <name>A0A8S1KUH7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0250159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUH7"/>
    <dbReference type="OMA" id="KFTYFTE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8056982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="132" mass="15625" checksum="95FC2F84C1980785" modified="2022-10-12" version="1">MNLSDAKRSSSIGKSFQIQSEHSSKDSNKFIYLTEIKIKKIDKSIHVPLNSWRELSLFEMLDEAIDLLKQNKSKINDQLKFKRLITQYQNCKQTNQIKQMLFQQRSKIIKSNLENIILSQNDFQNYLQDVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUQ5</accession>
    <name>A0A8S1KUQ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0270286</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUQ5"/>
    <dbReference type="OMA" id="MNMFLED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045238">
        <property type="entry name" value="Tim23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371:SF0">
        <property type="entry name" value="SD19278P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371">
        <property type="entry name" value="TIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8058567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="159" mass="17123" checksum="1E876A92EFDDBADA" modified="2022-10-12" version="1">MGSKAENQIEQYSGQDFGDKISFTVGVSYMLASSIGLLKGAIEGFPRQFNMPKKLILNNFFNAVGKRTSTYGQAAASASMLYYFVGAGMNLLFEDELADINQLKKNMLCGAISGAIYKSTLGIVPFFVGGIVGGSLIGGVTLLVENFNRRGIVAFEMKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWT5</accession>
    <name>A0A8S1KWT5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0290132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWT5"/>
    <dbReference type="OMA" id="DFLIEYH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="48936" checksum="313B827F12AEF389" modified="2022-10-12" version="1">MGSCNQCIQKQELQKSGRNQNVVIYQSAQNHKMAIKIQSAYRSYVTRQKFNLKLAEIRARNHIICNSTEDFLIEYHTCSYSPKINQFDGSNAFSSIQNNQKESALSIFSRQQSLKLQPKESDSFDFEELDQYNQTPQNKLIYSPNKSLRSLKNQKEKINSFKFRCVMTLEILETNDKIKLQQKNKRQKFDTIKLIGGNTYNGEWLDSLPDGKGKYTFSDQSYYIGDFMKGQFHGKGEFKSKEGTYYRGQWQYNQMHGQGIYNYNNGCKYEGSWEKDMPNGQGIEWYANGSVYVGTFLNGEKHGKGKITFNAGEIYEGEFQFDNFNGQGIYRWQDGRIYQGEWVNGKMNGKGILNWPDGRFYKGQYLNDQKHGFGIFSFSDGRKYIGQWKYGLQNGQGEFHKGDGSKQITKGIWKQGQLVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KY46</accession>
    <name>A0A8S1KY46_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KY46"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="228" mass="27353" checksum="938DF9F544C45F06" modified="2022-10-12" version="1">MDQIENDIQIMNNNHYYRFILTGNKNRYKSIEQNLELNKDVYSNKKPKHKKISLIPESQVFEYSHNFDNTSLDEHQIKNKSESNQRVNAELIRSKYQLEIQSQKSDLMTAYYSKLRQRAQSCQQKKLEKIAFVVDIGNSPIFTRDLKKSIDYSQSQKNKQRNMTDFQSKTIETQKFISQLDHFNQEKIMQFTFGLQYNKMNRLIQKSKFPKEIFLGDVRKVKRIFQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYM2</accession>
    <name>A0A8S1KYM2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0270243</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYM2"/>
    <dbReference type="OMA" id="RVCNICK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00065">
        <property type="entry name" value="FYVE_like_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052113">
        <property type="entry name" value="FYVE-type_Zinc_Finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39490">
        <property type="entry name" value="ARRESTIN DOMAIN-CONTAINING PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39490:SF8">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="14"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="551"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="636"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="559"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8058483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="706" mass="82597" checksum="55BE9F02EF0FC37B" modified="2022-10-12" version="1">MKGNIFHIDISSQRQQYKSCLICFKDFSVMTKEHQCKRCKRAVCDKCAQNKMIILGYSKSKDPHRVCNICKSESDILKQFVKTNIIQFNKDSLGIEWLKNLGVDIRQIKEQYRIQEQKDLLEYNQFINQLDSIYDEIDRKMNYSIRDFMSLVVQVDKGSVLGSIRNVLGTFLRNYPEVGFHPSLIFTTLILLCLSSEVTSYGLLQVMYTQLIPKALHFRNLRLGYDFKSELGVMIKSFHNLKIPTEPIKPFLQEVGPYIVSLTINAFEIQTSLFMFSQLFQEDGFNEFIKVLACSFSVHQFKDSIEKNKLFILWNVKDQQFQKYYYETKKLRSDSNSSVRSMSQSEQQQQLENKFKQQIILLQQENSSLKQEINVDKIKISKQKIEIDQLNDQIQSFEIELKNLRNQYQKIENENSNLLESNQNLKLQFMKQEKANESSSFTLSTKSDSTHLENELLKCKQEIEVLKLQNTNPTQMAQLVKLKNEQIAKLTQEKSELEKKIEELEEPKISKRTSSVAQISSVIKDLEDGKNLLEKKNQEILNLKKQIQELQQQKKSDQSMTSSQTLVDNPQRNSKVDNEDDISDDLQLLIQDSDPAKKIAARNVKQMIGKLNEELIYYKTQAKLKKEQVEPFRQKSESLQKQLDYLVEQNKELQIEIEHKQDLIQELEKKRVKQTIDPRPQVVQEDRKVLNENKQSCAANSDCKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L178</accession>
    <name>A0A8S1L178_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichohyalin-plectin-homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0310295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L178"/>
    <dbReference type="OMA" id="MAPFDIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019">
        <property type="entry name" value="KDA ANTIGEN P200, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="189"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="517"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="65"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="565"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="279"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="537"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8061409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="638" mass="79110" checksum="0424B071B58895C8" modified="2022-10-12" version="1">MHIAPVSLLNFDQTQIRDPIINSPRSLEACRRQGIKPEQLIMKSIYDLKRENKNLNLDDEAWKMLLQHEEDKRQARVQVLMAEREEIIQKQKDDYKQRKFEKVEFEKEKKRMEMIKKRREKQMQKILDEELKTQEIQNQLRQKEMELRQRQKEQQQELEQRRKDKKIEEEFRKQQIEYQEHLRKQKQYAEQDRLKYETNNKITPQQRIQQEKELKKQKKKRDKQLKKAQEEREEKERVRQQMLEQLYEAKLQKMEQREARRKVLLEEEKELRQQLIQEKQNEANYRRDENKRRAQHQQNQVYLNFQQKQLNAEQKLIAKQKEIELMNKEQEMKREIQKQKRQQIQQKNIAQQEKRRDDLIFKFKEIEDRFKINEMDRELTLYQKRQLQQEREHYRQQLLLKNDQELQKKCEKINMEREAKELQAVKVLKERDDMIKEKHLKELLRKQEQEETQERMMRIRDYEREQLKKELEDKSKRRDQIIKDKIASEKQKQEQKKAQIMEKEQIKKDLAELLKEHQQQEPKPIIVKQKKRAESAPQKSKGLQEQSQKSKPIVMAPFDIMTKEKQIQEIKIKQNQELLNLLQEEENKELERQKLLRQVDDPVERMRLEKILDFERALTQDRLAQLDNKHQKELEKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2B2</accession>
    <name>A0A8S1L2B2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0300068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2B2"/>
    <dbReference type="OMA" id="WDSSSKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1019"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="227"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="312"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="340"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="397"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="512"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="596"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="629"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="757"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="801"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="846"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8060305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1147" mass="122223" checksum="6F4E79BF0455887C" modified="2022-10-12" version="1">MNIIRLQQILKKENDSEHDLQKQMSMFVSNLLDDGKLISGFIFIWEFQQQIQEILMNRMQLNLKQAILQNNPIIHFIKLAQINLKIGPLTNQLNIHMSWNADNLEEGELKPVDSTPVWGGEQSAGGWQVDEVKKTQDSKQGHDEIKENKDSNGQNGTAVESGGWGEQKQQQQSSWGETKADNNDNAWGSGTTGFGSTSTADNGGSSWGGGSNSWGSGGNSDNQFQSDRPRGRGRGDRGDRGGGFRGRGDRGDRGRGDGFRGRGDRGDRGGFRGRGDRGGDRGERRGGYRGGRDNGGSWGGNSNNNNGGGNTWGNNGESNSWGNSENKDNSGGGWGSTTTNEQPAQSGGWGSSTEQPAQSGGWGNSTQQPAQQTSEGWGNKTDQQPQQAEQTQGGWGSTTEQPKQSTGGWGQTTEQPVQQSNGWGSSTQEQPPQQSGGWGSSNTEQPPQQSGGWGSSTTEQPQQQGGWGSTTEQATTSGGWGNSDQPAQSSGGWGGSSDQQNGNSWGGSGDGQRGRGRGDRGGFRGRGDGFRGRGDRGGGDGYRGRGDGYRGRGDRGGGEGYRGRGDRGDRGGFRGRGDRGDRRGGFRGGRDNENSGNGGSWGGSSNDQGGGGWGSSTTEQPATSGGWGSTATEQPASSGGWGSTATEQPASSGGWGTTATEQPATSGGWGTTATEQPAQNGGWGSSKTTEQPALSAGQGSTATEQPAQNGGWGSTATEQPAQNGGWGSSKTNEQPAQNGGWGSTATEQPAASGGWGTTATEQPAQSGGWGSTATEQPAQSGGWGSTATEQPAQNGGWGSSDPPQQSNGGWGSSNNDQQQSNGWGSNNHQSNGNGERGRGRGRGRGRGGDRGGDRGQNRGFDRNNNNENGGGGDFPTSKRVKPNPVVITISDDENGKASPQFSEDEQEKANIINEALKQAVDAKPIKLQLPELDAKFSPLFNPEPDPDSLKTDNLCHLKGGLYPHEQYKKVKFVYTGGIKVDSDRQPVVNLVTFDNDLEFIESCPKVDLAEKQGIETVFLSPLINEYRILKNELVQLRQEERRTQIRRQYMGVVEGIDKQNQDLICQVGLALLKEQKAIKLKYGFLFAELTLFHIKQVFGNLDYAEHQSLQAIEKELRPTNLVFVVSITQKWLNDVPKLKCRRRKELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L342</accession>
    <name>A0A8S1L342_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0290239</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L342"/>
    <dbReference type="OMA" id="WDPDHHA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09071">
        <property type="entry name" value="FAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07993">
        <property type="entry name" value="LPLAT_DHAPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000458">
        <property type="entry name" value="Fatty acyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033640">
        <property type="entry name" value="FAR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022284">
        <property type="entry name" value="GPAT/DHAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045520">
        <property type="entry name" value="GPAT/DHAPAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041728">
        <property type="entry name" value="GPAT/DHAPAT_LPLAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563:SF17">
        <property type="entry name" value="DIHYDROXYACETONE PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19277">
        <property type="entry name" value="GPAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03015">
        <property type="entry name" value="Sterile"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="6">
        <location>
            <begin position="599"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8059913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1138" mass="132017" checksum="C2AB19EC1894CB96" modified="2022-10-12" version="1">MLQEYYNRKVLFITGCTGFVGKVLLEKTLRCLPNVRYIYVLIRQKKGSSLMERFKREILDSQCFDRLKQIYGGGFEKFINEKIIPIEGDMLKEGLGMTENDKNIIIDNVNIIINCAASVDFNARLDDAIQINVRGPQRFIALAQQIKNLENFIHISTAYVNSDKAGYIEEKIYDVNQENLEQLVSQLLKTPVSILEKNVKDIIGDFPNTYTFTKCIAEKLLVQNRAPNFPLTFVRPSIVGASWKDPTPGWIDSLVASSAIFFFVGLGLIKTLNGDECLIGDQVPVDYVADFILAAGAYQNGRKEVSVYHCCSSAKNPMTWQLAKEVNALFWTRSPSSQQFSKPNLTFYKNEKIYKIMSKVKNAPALFYYQIANKIGNKEMKIQAKRLKKIIDRAESINDTFKPFVINEWIFDSSNSNVLIKFLNESDKQHFNIDIEQLNWRQYLERFNWGIQKYILKDQTRELSEQSTDVLSQRNKQSYFSDIEWCLSHGQDFKTKTTKEQISLVMNSQRVQSVIKQLVEEKSKKYQQTTLNLEKLHQNIEKQGIDICEVMFANYNMGVIRMFAWFITKVFRQIYEKVQINEPALLELQNYDQKTRGPLIFMPTHRSYIDFIMCSYVFFSYKIKCPHIAAAEDFLSMALIPTILRASGAFFLKRKQLEDNILYKTIFYEYVQRILIEECYLEFFIEGTRSRTGKTLNPKFGLLSIVCDAVFDKKIIDATILPITINYEKVLEADTYPYELLGEEKVKESLLRVIKAIKILSSNFGRIHVGFGKMISVKEWSKQQGLDSFENIRDRKKGVETLGYEVAYQLIEEMVVMPTGIVSTLLLMNRRGITEDQLIKRFEWVLRQISKRGAKTSITNNGQSDVAVRNSIGFLQDLVDKKKKNVFELTLIPKQEYKSILLLSYYRNQLAHIFFSEGIVCCALNGFGHLLSHKEGVSIERLWEESDFLLKLLKREYVIRNRITSKEQMINFIQQMIDKNVLEKVQEKIRVNQQFGEQASQFTCSLIWPLVECYWSTIVYLYQLKRVSDTNTTFELSELITQIQGFAEQMYGEHIMEHYESCSIETIKNAINTYLTMRCINISKKDDSDQVSIVFTDQELLEVEAQVKKYLKNNYSKSISNPIDIARSLLDYTIIPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L698</accession>
    <name>A0A8S1L698_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0340093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8062989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L698"/>
    <dbReference type="OMA" id="CPKKQCM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="26709" checksum="B87EFF62576BE3D2" modified="2022-10-12" version="1">MNDELEKTANFFFPVDNQGMKQKFVHTRRLLKHPVQSNVSNPSTQITTSKRSSDNSGVEGQHTPPQISGIRKRSELQISSEVRQSKNYRIFSSNINQRSSYPQQFSFGDKFQFINEFIQMKDEQQSPPFKQLKKLPEIFQQRNSSQGFRQALRIHSGQSKRETMSNFVQEIDCPKKQCMQQLQLKQTINKQQLRGTQIKLPNIGQPVSFLQTKQQLKMINRIFSGKRDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6G3</accession>
    <name>A0A8S1L6G3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0340118</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6G3"/>
    <dbReference type="OMA" id="FHQERAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="441"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="SAC" evidence="2">
        <location>
            <begin position="78"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8063039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="483" mass="57183" checksum="037787D36F9F7E4F" modified="2022-10-12" version="1">MQKEIQNYIGEFSLDYTNAAIFGKIQLQVHKYLILIVNSQCIGQLLGHEIYLIKQLVFYPLSESEVIHKNDQIYIEMIKSILCDDSCYYSLTYNLTHSLQFKKNRNRFAINHKYLEEIENKTYVINGCVQIYNNATLTYCQIVRRECLRQGRRFTQRGCDQQGNCTNYTEIEDVYQINKSTYSFIQVRGSLPFKWSQLPYLNPKPNIQIDSNSDNNIELCNAHLFTQIQYYREMFILNLIDRKGKQQKLGNYFQSILESFQRREIQYFWFDFHQERANLSKLVDEISSVLEQYGYYQIANQNTKLISEQKGVFRVNCMSCIDRTNVVQYLISKFILEEIIQDAGIEDSLEFIHKKVWINSGNQLSKLYSGTNSLKNNYIRYGKQTKYGMIQDAVLSTKRYFLNHFNDYHIQNSIDLTLGLWDMNDSRFHNYKFENSLQKCILIFILLMIIFNYLNLGILSPALSLVVLNYFPQIYQVRSILTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LAD3</accession>
    <name>A0A8S1LAD3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0360311</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LAD3"/>
    <dbReference type="OMA" id="HIQHIGW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000721">
        <property type="entry name" value="Basic proline-rich protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051412">
        <property type="entry name" value="Formin_Homology_Diaphanous_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691:SF4">
        <property type="entry name" value="PROTEIN DIAPHANOUS HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="2">
        <location>
            <begin position="342"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8064808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="412" mass="45990" checksum="9FC42D7352CDA671" modified="2022-10-12" version="1">MTDILKNPKVRQIIQDVVGGCANQTATVLEMTFVQIYTADEGKHKFHGSNIAGILCFLIDRREGKCFYLRVYDPATLQVLFSMELCYGIKDFWQPVHGIANFYSIPISKGFIGFLFPNVDEATKITQKICSNGPTRQQLQEINAQIEKEEKERKRLEKLEKEKGTLKGGFKDFFGLNKQVEKQVQVSGPSGFKRNQLTFNLDTGEINLDELPPELINAFQKAGITKKDLQDKNQAKEIFKAVANFNENPNAPPPPPPPPPSKQQQQQQSVPPPPPPPAKGGAPPPPPPPPPPPPPPPKGVPPPPRGPPPPPPPKPSGQPQMTQPNQQQQQQAPKQQPKSQSNKNDLLAEIQQGVKLKHVEKNECKVEIKEMDKKQQDDLSSYLAQMMAQRREQLTKNRKDSSDSHNSGWSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LEF3</accession>
    <name>A0A8S1LEF3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0350218</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LEF3"/>
    <dbReference type="OMA" id="DRVHLCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LITAF" evidence="6">
        <location>
            <begin position="82"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8063943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="167" mass="18909" checksum="A85B343AE9FDA6FE" modified="2022-10-12" version="1">MEYNQNNQYGQVSAQQGGYPYFQPQAQQNQYAQPQPYPAYQQPAPYLDPNIPVGQPVGQPQYGNYPQQPVYQSGLVPNGPIYTAAPVLRSAEGMRFPVQIQCPYCNQQGVTRIENRVGDGTICASILVLIFCWPLFWLPCCLEDCKDRVHLCIHCQKCVGKKRYEVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LF37</accession>
    <name>A0A8S1LF37_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CKTYENE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1146"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1373"/>
            <end position="1407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1415" mass="174058" checksum="C2ABF60FD9A03744" modified="2022-10-12" version="1">MQHQFACPCTYCKAKKDNPQQSSHDCYQVDRENEALKQEILQLQSQLHYLNQKVPEFQQIFKDNQQMKKALDQMNQSVKEYEEEIQRQKQVIDRLNLDLDKAKRDNEQLLQGFNDIKQRGLSQEQNLLQDNQRMKYTIDGLNNDLRNLQQEVHRLRSLNEKQKYIQDESYHYQQENQQYLTRIRQMEIQIRDLIQKEESYLLQINKANQGNNTENLLLKEQNVQLQQQLQQLQRQYQKQLEDMNQERDRIVSDQRNTINIQIKDQLLREQQKWQNERDALLQEIQLLKQQFGQNEQQIQRKRQQLEEEVNRNQQYLIELEQGNSQLQNKCRELQNQISLFEKDKHRTDQYIRRIDELQSQLDQLQRQMYELTNKYNNLLQQYEQKKHQIEQLNNQLNMVQDHDKQKIINLEKQIRELQDQLADTQRLRQQLHQMNENYSALQLTFKTSQKQLQDFDEVNQELIQLQREVEYYKQQDIKNKQDLDQYKSSLLMIENKQNQQGNQELDKLRYQNQQLSDEINNLINTNKQLTIKVQQLQNDLQSQQTLSERQNYALNESNQLKAKIKELLDQIQYLQIEINKKDKIIQELQIKLDQQNKRNDLLIIMEKERKDFEYRIIQLEKQLADSNIYKSQFIELQGKYENIRKDYELLRIKYEEINNNQQKIQWLEKQLTEVSIYKTQYLELETKNNNLIQEYDRLKKEYEQLRFKFDEANYQFKISQLEKQLQEANMYKIQYVELQNKYTIITQESEKIKKDYEQLRYKFEEVNNQLIISKKQITIQFNTEIDALKSEMQTWILKCKTYENEINFLRSTITDYQENENENSLIKQELERWQIKCTEQEREKQQLRQNLQDLEKLKYRINDLEKDNQSYQFKIVRLEKEADTTRQQILMLEQQRSQYDLIKKDYDTLQLRLKEQDRDKSQLRNEIDQLRQQLQQLKQQSQMLEQERNQSLMKFEESDRERQGLKQQIMILNNTKLEFENIRREYEIFKQKYEQQERETNNLRQQLQILNTQKGDFERIKVEYSQLRMNLEEQDRERISLRSQLQLASNQKNEMEKFRQEYDILRIRLEESEKEKNQVKGQLQSYIININTYKEQIEKLNIKLSQFQQDQGSFQQQEIMMKREIEMLIQQIDIFKKDQQTKQQQIQEFQFKYQEILREYEILQSKQVNVDVDALMYEIFELKKQLAQKEKQYEIERQEFIEQKFQLEKNIKIRVEQELRQMNEKYIIEIEELKKQIYIEKQQKLQIETELRLKYEQQILILQKQIKPVIDVDALMYDIFELKKQVITKDQQLEQLKKEQYQIRLQIEQQFNQQRLKFEQELKLKLERELRISIENEIRYQIENEYKFDQNIQDYILQIEKWKSVCQMRDEEIDHMRGLQQALKDKLNQYTQEIESLKTQLAMNQGDRRKGYQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHE6</accession>
    <name>A0A8S1LHE6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0410043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHE6"/>
    <dbReference type="OMA" id="LNGCRPN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="144" mass="16366" checksum="64CB03AD42CEEBE2" modified="2022-10-12" version="1">MQSANTQTYSYKNISSEIAIRSRGTSFERFQAYNNAQLLFTNQRLRTQLTVSKQGSQSNIGLMNKTQNKKPTLKITTHIYNNYLLNGGKPNKKKSLPILHISPKPLPLFLISDSKSSSQKACTTRTCSQVDLKKFQFKTLFNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPH6</accession>
    <name>A0A8S1LPH6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0390077</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPH6"/>
    <dbReference type="OMA" id="RADSHEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000210">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000524">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="53"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8066416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="380" mass="43078" checksum="792E4AAD2DFE1618" modified="2022-10-12" version="1">MGNCCPNHDLKTSFISSISDDKTKLATNSASKQPALVVIPPPPLKQQISEDDFIRIAVLGQGAFGIVFQVRKKNTDKIYAMKQIKIQKIRECNLETNTVLERNVLKQSKHPFIVKLKFAFQNPKYIYFVMECINGGQFYNVLKCKKNGLPENVVRFVAAEVVLALEYLNTKLKVIYRDLKPENILLTETGHIKLTDFGLATLRKDDEKSYLLAGTPEYLAPEIITRQGHSYEVDIWTLGILIFEMINGNPPFNDPNRRVEMITKQILLNDPTYPKTMSSAACDLIQKLLKNDPKERLGVKGGYEEIKNHPFFNSISWDVIHLQVSPLKTFAEMSNLKNSTKIINPLPQPLDDTPCNPYIQNGKVDGISYPEEGETFNSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSJ9</accession>
    <name>A0A8S1LSJ9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0430220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSJ9"/>
    <dbReference type="OMA" id="QKEECNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00065">
        <property type="entry name" value="FYVE_like_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="138"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="251"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8069145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="262" mass="30798" checksum="C73F2EE0E3B45A8D" modified="2022-10-12" version="1">MLNEQIVYPEPKIAIEFDNKLVCHICDDELELQNHCDFCGKSTCSNCIVKRRKLKEEVLHPVCEYCERIFVERILILSAKKQKEELAISLESANVELINRRQQLYQYKNQLHQNQGTNASEQKIQKLDEDIIEATFKKNQLLLQLQEIETQKIELKQSNIQKTQNVDIKQIKLQETTNKAQKSKDELEDIQRQIDEFLANLPMLDAEQKSALTQLDFSNYEQIFRGSNLPELMNQQAIKQSQIMQQPNSKKKQEQKEECQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSW4</accession>
    <name>A0A8S1LSW4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0450088</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSW4"/>
    <dbReference type="OMA" id="QQRLFNK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="3">
        <location>
            <begin position="161"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="185"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="221"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="257"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="295"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="331"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="400"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="443"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8070159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="514" mass="59426" checksum="BB8EC7673AC287EC" modified="2022-10-12" version="1">MSAKVYEAEDDLLGSILKNDDYEEVFSNNNSPLCKKSFESDSVIENVSTSEKHYFESFNRSFEQISLGSDLGQSPMSNRVMYPQCPLKNFKFSRQDDRQSVSISFDGPSFSTQESSINYSKIGNGYQYKQHNRFVDYQVPYSPGLLRPQGIYQPGFNYQQPQNGISFTGYQQERQPLHEINDDIQLTNDLDNMCGNQVLSRKVQKIFEHGKPDQRELIFNKVERSCVQFSKDVFGNYVMQKIFEKGTPIQQQRLFNKIRPHAYELSKNNFGCRVIQKIIEIISNNESLQNQFIDSIRLQVKSLLYDSSGNYVILKCLEVLQKEKIEFFLQPIEDSTLFLCSSQYGCKILLKALELFTPIQTDKIITTCLQHQQNLCEQEFGNHILQFAIKNSNYQPFVVNFVLSNFEKLCMNKYASNTVEKVVEASTNELKQHIISILISKSQINGLAIFVNLSINPFGNYVIKKMIIMSNHNILQPLIDILRQDQYLITAIRSSDFGQRIYQLIEKTLLMQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTD6</accession>
    <name>A0A8S1LTD6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0480097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTD6"/>
    <dbReference type="OMA" id="KLRCKEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="419" mass="49388" checksum="4AE51E422B7A8922" modified="2022-10-12" version="1">MNNFILKLSCKVHQENKIIGICQNSSCLKGALYCIDCLTLDHSDHIQQTTNVSDLENRLEEFILSMLINSRIRGSLNSNFQIQELFKDIKIQTQQLDHQFCESISLNNPNNNIEQNELIVHQEKDKCFSNEEIVDLQQKIECVKCKNSLEQSEIKNDVESNICKSCLGQKCSKCQQFFNYSLQNIENEIYCKECIKCHECNKQKPRQMSGSFFYHEDCLKISQDSILKKEMIAYIKKSFGTKLKKADKDNSITYFQSEPYPCLNIKDYSGYVIGYIHPLIKSNITIDPKIYKITQEMADKYLNEDLDQFEKQLYEFYLRQNKKPQEIPENMGMLLLFVLFIFKDFNTILNLALQQSYNHLYVEITKIKYTRQEGMNTIRGRVEKALDFFNDRFHREDGSVRQKDLKTQMKKFLPSFKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LX58</accession>
    <name>A0A8S1LX58_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0480209</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LX58"/>
    <dbReference type="OMA" id="RPEDKYN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="731"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="793"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="820"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="852"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="886"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="10">
        <location>
            <begin position="35"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="8">
        <location>
            <begin position="149"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="9">
        <location>
            <begin position="748"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8072097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="922" mass="104713" checksum="E0F32137D872DAF3" modified="2022-10-12" version="1">MYKVSKEQLREILASGLTQNPQNSMNEFNTTKSFENSLKCNITQGLSDNEIKQRQEVFGINQKENLDSISFLKAFCIILLQLKSIFFLTLSLIAFILKYLSTQGIYTNEWIESLFIFGLISLNLIISAIHLKIADNNKKKLLIQDEQLKQVKVLRNGQEQNIIARDLVVGDIVILQEKDKIYADGLIVEQNNLEIDECYLTGEQDLIHKITLEEATQQKQSILPQKHLIFAESRVINGNGKQLVLGVGIETQASRIQICIKQENNNTPIQSTIENISSKMETIGFVGAIIVIFMLLARYMIQYNDSKVNGYSTFSNILNLIIVLISGNASKALITHYQLQLAQTVKLMLYSQNLVRNLNQLENLPFMDQLLIDKTGTLTQNRMALNCFMNDNTEELTKSRFNQFPQEFQQVFIDTCFINNQYNPETDQGSFTDKELYQISQEMRINLEERKKQVTHIIPFNSARKRLTTIINNNRVVVKGAAEIIFQQSIKFYSLKNGIVQIDDFLKDTLEQSLIQLSQDGRTIAIAYADVNLQNENVDELIKNNKFQFDKQNLTLLGFFQFSDPLRIESKMTVEGLKQAGVKVVMITGDNSVTAKSIAIKAGIMSLDSIITEGAEFTERIEKKAENFSKYFEDIYVMSRARPEDKYNFVKELQQLGHVVGVCGDGTNDAPAISKSDVGFSMGISGTEVARETAGIILLDDNIHSIYKGLILARNLLDSIKRLAQYQITSHFSLIIILIASSAIGDAVISPLQFIWINLITDIFATFALSYCRPSSYLYHQNPPNKNGFLLDFIVYLHIIILALYIITVCIIFINQSLMVFNLFVMTTLFNLINSRMVYLELNVFSGFFGSWMIYTSILIIGLLQFLIVEYGGIIMQTFDGLNLKQWLICIAVGIGSVIWRTIAILIIRPIIVNNQLYILEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXS1</accession>
    <name>A0A8S1LXS1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0440110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXS1"/>
    <dbReference type="OMA" id="HMILVAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="351" mass="41376" checksum="39C77E0CF2FDF7EE" modified="2022-10-12" version="1">MSLEQLQCIEDDKQYYQSKNKSSHQQSIQSITTLQSDHSIQSLEKLVSQDIVKDTNLKLELDQFIGLLQKVKPSNLKNELSVVMSTEQEEYYSFQQKQPLDKKEIMFEGFQKCYIEAIKIISNLIRDTKEQNYIISYLEKMQMIYIMHIQSYQGMEKSINLLNSLKQTLIDADQEITLLHNQSAEQQSILQKMQQQLKQNMSELNQERLNSLDLQQKNQQLKHMILVANKQFESLQEKLLQTEQELNEIVSTQSQQIKQLVQTNKNLREAIVFNEKKQEIPKKTNTSPLNSQRSTTNSNQENQYNQLNNQLTINSQRTQNLDKDTQNISPQQSQRCTSNKREWNKYIKKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M2C7</accession>
    <name>A0A8S1M2C7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0490137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M2C7"/>
    <dbReference type="OMA" id="CDFRIDW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="26541" checksum="7B1EF70F40B06D9C" modified="2022-10-12" version="1">MNNISNLIGQRRDLKQSKQSPSNNKQTSQQIQIYDQLKNSLKCDCQKQNNKDYTQYQIFEYKTQKELEFLMQYIQQACIHNPQCIHEQLFEKNQQNKKFRQLCDFRIDWTKLIIKAEKQFEKAIVKKQMAINYQPNNDCFKIIILEAINNKENSLKMLIDKSKQELQFIEYAKRLAIKLQQKLDCPESLLCQFEDVQNRKKYIHPPSIWSIMNCSCNTTSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4S5</accession>
    <name>A0A8S1M4S5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0470270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4S5"/>
    <dbReference type="OMA" id="NTQMHLK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="627"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="640"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8071616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="660" mass="78014" checksum="B3EE0798C3DAD323" modified="2022-10-12" version="1">MLQKFKDGLRQIDIFGQSINLSFRQEEQYKTSMGGFLSICIIGTIISFFYSNIINFFAKLNVTSTQEFTFADDPDALILNKDHFMFAVQIEQDNFTTNPFFNITVEQRHYYRFPNGTQYRHPNKYIDLVPCTLEHFLPLFEQYSVDFREQFEQQNLQNFLCPNLNFIHSLNLTVGGVWASTDYYFLKFSVTNCRDSSSSNFTWKPTCKTSDEIQSTLNRQGSFRFQVYTTNFLLNPNRPENYVQPYLAVDQFYTFVPDKMFVQSDIFFRTKKVTTDQGILMYPDKQNETFAFRDYGDQREQFEISRITPNYYAAFYFQRSPYSYRINRKFLRLDELLSYLGGFTQFMIVVVGIIVRFYNRQHLIISIANDLYEFDMSQGRQNTQMHLKNLLDRTQRGREMLKSKMSRKIDTIPQIQTITAFQNKPDMVQKRKLNVDKKQSIEFFQPTQQALQSRLSAMKNKSLQYFDDFKEFLKKKHVIGLGFRVMLSSIIPIESLKDDDCIVLQRAIDQVNKELDIEYIIKQLHELTKLKKVLFTSEQITLFNFSRKPKISLIQDKNNRRSTRCIIDGVTTAEDYGVMKQFTDLVNSYDKITGSEQENTTQEQIRFNQRLIILLGPELMKVLEKELSAQQQEQMESDNNDPKDDENLFSDREIQVHKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5J6</accession>
    <name>A0A8S1M5J6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0500255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5J6"/>
    <dbReference type="OMA" id="WNDYGYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18773">
        <property type="entry name" value="PDC1_HK_sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="526" mass="62175" checksum="764A98239F20EE64" modified="2022-10-12" version="1">MGNKFWKDWHLRTQICMIQFAISILTIGLIAISTYSFMFYISDFYMEASEHLFQKQYGQWTKIMMSHNIHRVQQTLFRSQQQIMKVNTLYEMTQQIMLSNLQQPTSCLNELKLMDDYAYSASFCYMIYKDTQSNKILQNLKDLSAFLTESVHIIDQDFDIMIASSNDIHFFTVWPGSFLSSDYNPQERIWYQQHLEQLALNNNNNNSTYYSEPHIHWTWKLLMIAQTKSLLNIDGSLDGVIASHVNFSQFNYSDDQVSFTIINPTGRILLSSKNVTEYSHIYDYNITNLGYQDYQQILNQAQGKPTQSNCDSTVWNDYGYLCRKTYNKQEEELISTKSIEETGLIFIMQSKLSKYQIEFKNMFNTFQSQLNNIFVGTILGFFFYMLSSLIITTCVVIFLFNPIIKIINYTSQQLFKNSLNKKNFLQKQNQFSFFQQLSTSQLLNDLQQSFNRLIHITTNQNKSTICYLIEGFKYPYKRWKQKRSNKRIRNINLKLKDNLQNEETHKLQMQIIKQLMISELKINGCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8Z3</accession>
    <name>A0A8S1M8Z3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0560238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8Z3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052775">
        <property type="entry name" value="IUN_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190:SF1">
        <property type="entry name" value="SI:CH211-201H21.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190">
        <property type="entry name" value="SI:CH211-201H21.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="156" mass="17458" checksum="64723CD9ACCAB636" modified="2022-10-12" version="1">MYPAIVDKLGCLFAKGGTYRGVGNVANSVAEFNVQTDVEATAAVAMGKFKQKMLLQFDVVLAYTLINKMEKSFLRMKLIQRNLNLLKVFSKLQVMMETTSFLINISIVSDGQARGQVIIDWLTLLRCNQKSIVSILTAFEWDSVIEIATKANQDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAM9</accession>
    <name>A0A8S1MAM9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0530182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAM9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="79" mass="9551" checksum="0836515E0E88FB23" modified="2022-10-12" version="1">MTEIEQLNYEQIIKYQTFHKKLKIDIEKALYKLIQLQIKQDDYQLVTEFIKNQKGQSQTKMNVGGNTFQNFEFLQTQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MB13</accession>
    <name>A0A8S1MB13_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0510230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MB13"/>
    <dbReference type="OMA" id="NKVEDEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="319" mass="37598" checksum="BD57A7F2265C7468" modified="2022-10-12" version="1">MSKNKSKEQVNNSQVNISKNGSQAQLNQSKSQSSINNQSVLNQSLKDELNKSKTNEQVQQENHEDKLNQSQQNKNYSISAKSWDLNQHEVKFLNELYGEIQEAQLLAEGLQDENKFENEQIELAQKDINTVQNVIEMLKEESDKEDDIRFAIDNALLNLATTKRKTEDENDKLRDLVRQRNAQLRMLVIKVKDQDQQLKQKDEQIKRLEQQVQQQGQTLRQLQNKVEDEYRIKVVQEQLRKPRENKLFDESKFVDVNAKDDPFDFWQNNAIREYPGEPKKLNDVVQKKTLWVAQKEDEKLNKFEYSKMPKSNESNFWGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDL6</accession>
    <name>A0A8S1MDL6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0600007</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDL6"/>
    <dbReference type="OMA" id="NMWAVFT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051085">
        <property type="entry name" value="MB_O-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285:SF18">
        <property type="entry name" value="PROTEIN-CYSTEINE N-PALMITOYLTRANSFERASE RASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="427"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8078358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="536" mass="64255" checksum="FF1F0E54DE5487CA" modified="2022-10-12" version="1">MRQQQSNKSFNISNASFYISIAFMIVFESYSLYRFSVICKSNKVEGIEQGYFGFLRDHSDHQWGQFAGHLNELMAFQLIFLIGSYTVKYYSKLESKQRNLQYYNIFLGMSYAFYLHNIGMIFQLLIIIGYYLFQKIFFKMKYFIPLLWTLALVTLWSNETFQGYSFTAISPSLKFLEDFKHNNELVRWNLVFNMILLRIISFSVDKVWASQQDAKYKYDLIHEKQILKTYRERVQEPQSLEEYNFLGYLSFLFYVPLLFSGPSMGYNAFNSQLKIPQQQMNRAQVLKYILRVYFVDFLTFEIFLHICYPNAIPKIASNFKILKTFSVFEFHIMGFTNLIFLWYKFLTIWRIARGWALLDGIETPENMNRCIYNNYNFSGFWRSWHRSFNQWLIRYIYVPLGGSKYKSLNMWAVFTFVALWHDFKLDLLLWAWIICLALIPEIALQKYFDKEFFYKKPWFIWLCRLGGGFQIEMMCLANLIGFGNGHEGMNFVLEQYMSWQGLLCFLFYCVIRNGFATTIQFRIRDDEKAESNEKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ME77</accession>
    <name>A0A8S1ME77_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0550200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8076033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ME77"/>
    <dbReference type="OMA" id="QIMIIDE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="379"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8076033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="544" mass="63469" checksum="40CCD2257C8B03DC" modified="2022-10-12" version="1">MQYHVSKQDILGTLQDVFIKNNHQQENQKHSKILQKPCSVIKSNLESSTSKNKLERYLEKYQQRKQLQDENNISIAQSIQNTNNIKVQQKLNDSQTAYSSKMSFPRENTENDDKEQQLTGVGLSQYMSQLQQSKLHCTFMNQYCQTEPQKKQMIIQGLNEFVQDHNESSNSVIDEIKLLNSKHTQQNTICQQIQLSILLQKEQSIKQDFSCQFNTEIKQQVDNQQINQICQINQENNILTQIDFGCQYDESNNQVSLILDDEHPKNIRNRSRCQTSANQKNKNNQINSDDQITFLEQELQLRDEMLETKTRIITDMQQQLSDFYKIFNKESDIEVGQQDLNSSKDSDHWKVDELNRQLENDVNIQDQLTQLMLKYSDEILELKKILDDTTEQNRKLKEEQEQLIIQITQLKQQLVDVEESKAKEIDIINQDYQDKLSKQIMIIDELQSQICSIQTSPTDIPLASIQTTMNNITEQHMDEVGCESQTTKTINNQKAKNVLKHINKMTIMTAGWMSNKQDYVTLGVLGRIDQIKSQTKLDSFKKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MF23</accession>
    <name>A0A8S1MF23_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Deflagellation inducible protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0590164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MF23"/>
    <dbReference type="OMA" id="ETKNEYD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033362">
        <property type="entry name" value="SSNA1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28661:SF1">
        <property type="entry name" value="MICROTUBULE NUCLEATION FACTOR SSNA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28661">
        <property type="entry name" value="SJOEGREN SYNDROME NUCLEAR AUTOANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8078099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="12510" checksum="CD7E4FC873393E82" modified="2022-10-12" version="1">MSNYQNLVAELIGTLEDLREKKEELKNEIYEEEEERRRIEKELEILSERLDRVNETIMRTATEKRELEKTIQETEHTKNKIVESLKTLLHVLQKESQYHIKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKT0</accession>
    <name>A0A8S1MKT0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0660015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKT0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8081477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="41" mass="4999" checksum="7D35742FE6DB7615" modified="2022-10-12" version="1">MLQKPKSLLRRYDDGTLREECDRYYLRGDGQFFKKTNNNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MMC5</accession>
    <name>A0A8S1MMC5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0590082</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MMC5"/>
    <dbReference type="OMA" id="TNHEYGT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08291">
        <property type="entry name" value="ETR_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF18">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="4">
        <location>
            <begin position="31"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="3">
        <location>
            <begin position="159"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8077935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="333" mass="37484" checksum="03DF09A13BC37087" modified="2022-10-12" version="1">MSNANTMSCLWKESKDAKPYVNRVPIPTVEEGQLLIRMDYAPINPSDIKFLQGQSSSNKQFPCVPGFEGSGTVVLTGGGMASWGMSGKRVAFYTNHEYGTYGEYCITDTNLCIELDNDMESSEAACSFVNPLSVIGMLDICKKNNAKAVINNPGASQLGKMMNRLFKERNIKVINIVRREEQVYELRYECGAELIINQNDPDFLKQLKIMCEATQASIYFDAVGGEQSGQILNIMPKGSILMMYGTLESWQIGGIQANNLLQEKKSIQGFFLNIWLKEQNKLELMVTLKKLKNFIKTSLKTKIAKEFSLDQFQQAIDYYKSHMTDGKTLIFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPC3</accession>
    <name>A0A8S1MPC3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myb-like DNA-binding domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0600164</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPC3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="59"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="59"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="153"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8078615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="42062" checksum="7CEECA3061FF8044" modified="2022-10-12" version="1">MKECRQNWNQQEDQQLQELHQIYGEQWKKIAELLHLSHKSQGKIRSAAACRERYQNILKPTLNKNQWTREEEIKLFSLQKLYGNCWTKITEKMKDRSELICKNYFYATIRRVLRRLCKQVGIIKPSLMLKSIKPSVLMSIYMENNQEIQLVFKEEVRCQLNQLITKYQFIQKEENIKIDNEEIQNMRQLIQELVNANQYLESKNEVLLLRKNKTNILMINQDQMQMEKETIIKRIRLKQNIFVIRQPNFNYQKQQKEYIMKNSSINENNNNYSQLIPIQYTQIDENQFQNQENFNLYQTQAYFQNYMAYSKYVSQYYQQLVNYYSPYQYQYNQYNNYLLQQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MT97</accession>
    <name>A0A8S1MT97_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0630029</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8079975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MT97"/>
    <dbReference type="OMA" id="MTWLGER"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18557">
        <property type="entry name" value="ABC_6TM_TAP_ABCB8_10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000215">
        <property type="entry name" value="ABC transporter B family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000218">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF1">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002773">
        <property type="entry name" value="ABC_prm/ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="57"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="371"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8079975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="615" mass="68795" checksum="7013C186939B8C2E" modified="2022-10-12" version="1">MNKIQKKMNFDADYQKIQEEKKSRTAIETEISIENKPKRSNFLRLLGYSWKHKLLLFLGNFGLLITSLSMVALPYLTGQMIDSITKSDGKEDLNNLTFYFIILTIISAIFTFVRAYAYNILGEKITFDLRNELFQKLITKDIEFYDTNRSGELISRLSSDISVINKGANDSISMILKNAVQFIGSLILLWFISWNLTLVLFCVIPPFTIIVILFVRSYKKLTKEYQQAVAASTQIASEVLGNMRIVRSFSTEEKERSQYKNATSTVYGIGNKIAILGSQFMSIGILLGYAVILAILYYGGSLVIEDKLTIGDLSSFVLYTLTMTISILAVSGFMNQIISAAAVSEKIFSIMDHPVKIQNGSLDAQHITGEITLKNVTFQYPTKSNVTTLKSIDLEIKKGEVVALVGQSGSGKSTIVQLLERFYDTNEGQILFDNIDIKEYNQQKLHQTIGFVAQEPTLFSGTLKENITYGVSTYTQEDIDNAMKLANAYEFVSNKSIFPDGLDTIVGERGVKLSGGQKQRIAIARALIKNPKILIFDEATSALDSESEFQVQTAIDGLVSTGQKTIIIIAHRLSTIINSNKIVVIQNGEIVEQGRHQDLIEKNGIYKQLIERQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1P6</accession>
    <name>A0A8S1N1P6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PPRIM_AZ9-3.1.T0770082</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1P6"/>
    <dbReference type="OMA" id="WFAMHAP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000585">
        <property type="entry name" value="Calcium-dependent protein kinase-a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000299">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001182">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 27" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="43"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="345"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="382"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="418"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="454"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAD8086758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="497" mass="57122" checksum="C7F46B5352AD21FA" modified="2022-10-12" version="1">MGCGSAKPSDVNNPQQINDKSQLRVTSLNLVTEKMGQISQDYHLLKPSLGKGAYGEVRKGIHKLTNQTRAVKIISKDKAKKADMERLKEEVDILKRLDHPNIIKIYEFYQDNKNMYIVTELCTGGELFDKIQESSSFSERKAAETMKQILSAVNYLHKSKIVHRDIKPENILYESSKPNALLKIVDFGTSRFYDPDVKMDQKLGTPYYIAPEVLERKYDEKCDIWSCGVILYILLSGTAPFNGDDDNLIMEAVKRGFYSFDTEEWRLISIEAKRLISKMLERDPKKRISAEQALQDDWITTYVKKPEIDLPQLTRVLNNMKNFNVEKKFQEAALTFMVNYLATSQEKQELLTQFQALDLNGDGRLSREELIIGYSKVMSYSDAEIEVDKLMKQIDQDGNGSIDYSEFVLATFNKVKLIEDKRLEQAFKLFDKDGSGTISIDEIKQIFGQNSQVSEKVWKDLIQEVDQNGDGQIEFKEFKEIIVKAIQNTNEIDDIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6H9</accession>
    <name>A0A8S1N6H9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6H9"/>
    <dbReference type="OMA" id="HRKQLTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="104" mass="11962" checksum="5C142A760CD1A0F2" modified="2022-10-12" version="1">MLHRKQLTIQLGLKSRQSSPSSQGSETPKHKKSNSYMQFSPKLDCNNGLSFVNEIKEVKITSPRTEDLQSPKSQSLKQIKFNNIQKEEQMKKSQFSKTKSDDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N8P1</accession>
    <name>A0A8S1N8P1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0810025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N8P1"/>
    <dbReference type="OMA" id="YRIKCHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="130"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="202"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="299"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="382"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="646"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="725" mass="85599" checksum="D0CDD14891EC5F4C" modified="2022-10-12" version="1">MNLSMQPRTISPTKYSQVLASQDFSQPGTVKKAHPLNNQSPYIHSPVQNKMNFSIVSEDAENYRIKCYELERELAKYKADNELQRLVQQKSGDLEYKVVEVLESNNALQGQLERAQKVALQRKSESEMWKQKYEAQMGSLMQIRQNYESEIKALTLEVQKANARSSALDQEKNRIISDQRNVADNQTTQIQETFKRSNNSQADMYEAQLKKLRDMLEDRTLQVAQLQSQIERQKVESQDTQLRLMNEIDITKSRLSAIQIDHQNELLAQKQKLELYQETNLRNQQTAHENQQDVQGSEIVKLKNLLEIKTQEIETLINQNQKQKLQQESEIQCLRTEIEMLRRQILSNEQLHQQENHNIQTNLDNIHNNDTTNLKLTHENQLQALHKEILKLKEIIDHKNQDIQKLVVEKQQQKDYYDGEIQRLINTIEDQKRKAILLENEKNREIADQHARIERLTIQYENMQIDLQKQIQLLNQEIINLKNLLDHKNQEIQQNLKNYSQIKLEFEDKLRNLQNELELVKLRAFETEKSKLQEIQDLKQLIKEIDTQNREKQKQLEQQIDHQKYEIQKTFDMYNNKIKECENLTIQRNNAELIQKRIQDDNDKLIEKLHHFEQEKNLEIDELRHKMDSGAAYQFENLKSAYNTQVGLLSDQIADLQNQLAIKNKELTEMFEKYTILDKSLLPETLVSRAAYNSRIGVPAELLANKSIHDATLKSQLRNSPIQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAG2</accession>
    <name>A0A8S1NAG2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0800156</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NAG2"/>
    <dbReference type="OMA" id="SSPAEWY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8088239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="226" mass="26676" checksum="09C3F498B3BDE613" modified="2022-10-12" version="1">MVLEYILDIPPLTRIIVISSIILSYATYVQYLKPSNLYLNYKLAFLESFQPWRILTSILYFGELDLITVLKLFFFQSISSSLEQHTFPGFANYLYYLLLNFVTITSIGLWLNEHSLTEYFVEALMYVWGRQNQERPLLFMFIIPVKAQYMVWFFILINIVTGRSIQSNLIGAFIGHTYYYFAYIVPKLPSFKGIHLLSTPKFLVKLCRIFDRQNFVHQEEGQVFAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCS9</accession>
    <name>A0A8S1NCS9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0860071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCS9"/>
    <dbReference type="OMA" id="KTHRIEP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8090510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="227" mass="26501" checksum="CE18C2AB334EA161" modified="2022-10-12" version="1">MNQGLNNKSKAIIIKQPYPTFMRALNLSKNTKSNHEIPKEQQQIATQRTQRTIIPSIKSISNKNSQEKIRVYDVFKQIPRNKSQSYHNQINKMDESRDILTKLNQQKIINVDCQKTSRFLEPVQTTHRIELPPSNKKRSYSNAQPIDKLFKMNDNSISYIKPPLETQRKISKIKTNLKLQRDDNILDLLLLNTCELKKKLSNKSSNNSSFICVRPGKLPKDFFIYNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDI0</accession>
    <name>A0A8S1NDI0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0800224</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDI0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="11"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8088376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="14475" checksum="A2E55A3F3A963145" modified="2022-10-12" version="1">MIKHTIIEAYIKAQYYIGTTLLKLNRIEDALQSFNTAIDLNKNNDEALENKALSLHLLQRFNEALKIYDQAIFLKKNSNRLKRKADTLLVLGRKNEAKQYYFEALRIGNLSSDQEQILYQLARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEG6</accession>
    <name>A0A8S1NEG6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0840046</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEG6"/>
    <dbReference type="OMA" id="QDNRVNG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000064">
        <property type="entry name" value="Protein phosphatase 2C 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="15">
        <location>
            <begin position="23"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8089649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="300" mass="33517" checksum="204DD2C8BFDFA0C1" modified="2022-10-12" version="1">MGPYLSQPKRDKVTTTGSGKSVIFAASEMQGWRNTMEDAHIHRPDIVQDVSVFGVFDGHGGKEVAQFVEKHFVEELMKNKNFKDQKFEEALKETFLKMDELLLTPEGQKELNSYKATDTDESYAGCTANVALIHKNTLYVANAGDSRSVLCRNNSNYDMSVDHKPDNPEEKSRIERAGGFVSDGRVNGNLNLSRALGDLEYKRDNKLRANEQLIIALPDVKKVELGPQDKFILMGCDGVFETLNHQELLKQVNSTLGQSQVTEELLRKAAEDLLDQLLAPDTSQGTGCDNMTTVLVYLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFB5</accession>
    <name>A0A8S1NFB5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0740114</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFB5"/>
    <dbReference type="OMA" id="FMNSPIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="461"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="671"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="764"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="814"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="672"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="708"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="764"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="814"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="825"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8085394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1003" mass="117305" checksum="CE00FB5C3D218F08" modified="2022-10-12" version="1">MQLSKKIEEFQDLDPIENISKRKISRTMGYQNSESIQEEDEEQSSSSMQLAKRKFQFMNSPIQQPCDFCGSDMKVPTIYTNIQSNKSFEKNPFQQESKIKCVDNNSASRLIDVSGLDPHDSFQSESLQLQLMDKNKSATINSLHVKNIVNKFIQQMKRKINWNELKDITLIDDTTQIQPHMYKWFTVSYFALTSAIILASIIIIPLQIAEEQGFEIAAYIIHGFVIIQIAADIHFKRGPFHMKEGKFNNEYLDHSKIVLDLSRLASIIILIFSYQYKIKYFIIPFIIMQLIRQAERFENIYTSTQLIVYILILWMALIMTFVCLLESDEGIHYSLSLAISILTHNGAISIDITPTNATLLQIFMIISSFNMIYTASVIFIWIKPTMKLEEEKQKHLASFLKGIKDKSIDYGLQCRCYSYLEYVIDEDIIKNRDLLIKKLSPGLQEELQISIRSKMVDKIKLFNKFSSSLKQQLIYWFDMAQYNPEENIVQEHQVEDFCLYFILKGKVKIQFQGYFQGKPKRTFHTLSEGQTFGEFSFISGIPPYISINSQSLTTVFKLRRSDFLEIIKTFPQDNEIFCFFKDNCNQNHNMFECHYCKTKGHLLFECQYLQYYPKKLNIIEKHIYPHPQKRFKIERKNKEPKALMMLFVVGERAKQYQQKLSQEVMTSEDFPMSSQLPYSENQTQHSASYMSKTQSVHKQSPEKLSDNQDSDGDQLYEDLANDPIFNNQLRKQQNKTTLRTAGFPFMSETLENLHKEKLQVITEQSQSKQLDSLNSKQEDTNQLSKQSSGSQAFTKKNHLYTAARSMHTKLTFRYQQQQSGQTEDQIQFQQQQQNSIRQTSNRSNTYSNPLSNNLSNNPSSNSKVNTKSAKVSPTQFQQPPPQKKKEERGGSHSDQSLSEQQRHTNRKKSTKTGTKVSILNQMSQFQIINTPENHHYQYHDILFNRFEKMHLFKYYNPHNNYDNVIIRINKFQRAKKIKPIHYTIKCFISSKIKKVKRILDSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NP05</accession>
    <name>A0A8S1NP05_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0890215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8092086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NP05"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="141" mass="16365" checksum="112A4680C9DF154F" modified="2022-10-12" version="1">MFNNYLCTSTFKLTLQKVRNCLVEKQLFFIITNRLECSYKGETCESQAERCLTCPLNSLCILDSVKGFFCPGEYYDKEDQVFCQNGKAQDNCLTCDSLAKRDLKIMNANVNHIFQNNLMHFVIIGIEQYECVNSYENCMKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQU5</accession>
    <name>A0A8S1NQU5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0910022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQU5"/>
    <dbReference type="OMA" id="RLCFIFD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="68"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="39058" checksum="635E62440EE25598" modified="2022-10-12" version="1">MGNKNMQEKNDNQQQFQYENLEGCQITQEEKCFALAFNQSCSCLVAGSDFNIKVFKFDYETLEQTQLLIGHQGYIQTLNFMKQTNQRFISGSCDSLIIIWELNILSSLWYKKQTLNYHILSITCLIINKNEDFFFSGSCDKTIKLQVMNHNQEWVCKQSIIEHSSYINGLSLNNDQNKLITCGQDQKILIIQNIDDNWIVIQKIEQYGLRLCFIFDDFFTFQPNGNFMYTYQLESNQQIVNYKQRKINLKRGGCVCTILFPQYFIQSKSLLVNKNGGYVHFVKILDQNRYQKMQTINFGTYQVFGEISSDGQYFVSWDDLSNQIQIKKYIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NRJ3</accession>
    <name>A0A8S1NRJ3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0830166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NRJ3"/>
    <dbReference type="OMA" id="CDEFGNY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="284" mass="33759" checksum="DB928AE328A21182" modified="2022-10-12" version="1">MNWSNVTFHLLKYESVRQKENEFEQQQQKTSLNNNSFSIPTKQEQLQEKIRCKSDQCDEFGNYSNKYKIFHDLIVEFPQHKKQRLTQIIVQNLSISFLIQKKIQSPFNKILLEILLLNHKDHKIMSQALLIKDQKILNIRNKKLIWIVKLEWIYIIRMSKKLLNKRNFLKLRLILALKLCILVSLVALQEKVLELKPLKVHSHYIIETTNKFILKQTLQKTNIIDKIICESKINQKTTYSPRSIVNHQHKKTNSSGHSAYSEQNKFLRRIIGNSLFIKTLNKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NV95</accession>
    <name>A0A8S1NV95_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0980088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NMMIVNY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="698"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="856" mass="101555" checksum="EAE53F592CFC7E50" modified="2022-10-12" version="1">MINELQNLLNTASNLIEYQILFFYSRSQITLTKLNILNQIVYNTDYQSLEKPQLCPYNATFFDSNHYYPLSCIIYHSLNDDTSYINSTQDYQLRNLSHLFNEMILSIDLTGQFIPNDIFMVSDSNPNEFAFYYPQWIKYKTYKPRKRSWYQQHYANLKLNPNNHTQLSEIYKYFNDSDVYSMTMSQSFFNQTKNVDGLFAADIQLQNAYFNNRNLNMMIVNYDGKLILSNYKNYLLANSTKYESFDDEKTTGFNKSDWLAILNFINHNKKESTCSSIYNTQKQLCLYNSAYXLYTYFCLKMTLFCQNFRKIRSNLKMRSQILLINAIIFTFTLCMLLIAYILNMGQSLSILNISSQTLMINELQNLLNTASNLIEYQILFFYSRSQITLTKLNILNQIVYNTDYQSLEKPQLCPYNATFFDSNHYYPLSCIIYHSLNDDTSYINSTQDYQLRNLSHLFNEMILSIDLTGQFIPNDIFMVSDSNPNEFAFYYPQWIKYKTYKPRKRSWYQQHYANLKLNPNNHTQLSEIYKYFNDSDVYSMTMSQSFFNQTKNVDGLFAADIQLQNAYFNNRNLNMMIVNYDGKLILSNYKNYLLANSTKYESFDDEKTTGFNKSDWLAILNFINHNKKESTCSSIYNTQKQLCLYNSAYQSDVFISAKQIKGMNYYLIIFNNIQDQATLFEMLNQLMETIYLQFKQQMLITGLICCVFIIISFLLIYLICRPMLKLIKLSNIYINSHLVSKSFKDQKLKRVWTNQEKFNNFIQNQVLNHNPEKTSNNLMIKLIQLFQRLFDGILNQQNKKSNQCFIIQSFNYPKTQHLDINISKQIKECQFKNLNSDDYNLLLIYTLKQLKRQFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NW24</accession>
    <name>A0A8S1NW24_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0950033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YITEYYS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1346"/>
            <end position="1372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1386" mass="163763" checksum="CA9DCB4D43425E69" modified="2022-10-12" version="1">MLLLISIFLRHIQSCNILKNELEISISTYENMYWEIKDELIEGEMTKFRLEGDKNGTYFKLQQPNDIYDQKHFPYQIQEIIKGRSYRKGEFWLNEYSLLGRDSQGFHIYIINDESFRQLSLYHLCTDFDYLDQNNYVVVCQDNDGIFIQIINKSGTIVFVYSKLMKQKSKYNLAIYRDKSITYLLIYIPAYAEEILNIQSIINVFLIDSYQIIETPYYLDKKKLSELFQNELKYFSVIDVQVYSNNVYVLDYKLGPIKLVQDNKGNFTQAKLITSSLYITEYYSFSIRNYDELIVFGFVQKNQMSLRVLKNGRYDNIVRTFEKGDFTLGICDVQITPKYYLIKSDKDLMIFNDNELVDKFQIQKEVFIANPYFDLYILFHSNKAEFYEISSGILMFQKQILKSENQQYFKLFDNQNDNCYVNIVLAVYDKLDAKIHTNYQTSFYNQRIFYYPPYPSTYYTWPRITNGPNVQQKITIHNDQINNIDLKFLSLSPMTITGISQFDQNNVIFSLINNNWGLNQYLVQTENLSAFIYECQMNELEQKCSQKITFYPLIKLVDDNIVWTEIGIYDSYIGIKTQNNHVITIYRVGDSKIVQIFQIKTSLQNEKTQITDFAINSQFVFVLISGMNEIQIYSLSLKIQIDTISNLNNAQKVYISNTFNKNLLFIKCNNIIIIGHYVNEFNIISQIQVTEQQNVQLLLSIFRSTLIVVFKTVTEQGILQYLIHNLNSVTLLRKLPLYYYQLSQQLQLASNPLKNLILITAYSVYTQTTVILIYQINEYQRNSLLTVIDTFNYFEGELNYCVAGIANTVIQFIYKGQVYSYLYFEQPRIYINYLTNPTHFQSNLILTQQEWNDYYSLKREYNKNIIVVDMQLEMQWIKDNIEFEFDECKEVQTIRIKNNWIQGQRIQFKLSCILCTTDNDSGQIYLLNQFLKSNRQIQSKSIQQFINFDDNFLLILSELTGTKQYLQFINNVGEIANEIELQIEQYNYVATKLFLLTKNILVILYENVTAQKPELRYYYCWKDQEKQDCKLMGKIELQDRRIIKEMTYLNNVQMILTESLTEYPSIYFYSLLIDNKVFTIQQFNLIINKDYFHESFNITSFDVMKISENQGCLVLAEFSIGIIAIKMTLTKDQVSISQYEYLELPFKQQNLVEPQENRFIKIQIFKEGNNNCKLILVDQINESYQIFLEYYRGKFKINIAQVFLKLGNIIKLSSINVIDDYLLQLFRYENKYYFCLYEINQNNDVRNNAGICKAGLKQSDYDMIISSNFITRKNQFGKYQLLINTYDDRSLQIYSIDQDARIVVKNASELNNYTVSLIAVNPYKQVQLGINLTLISCPNPDQEMKLFFILLIVVLGVLFVGIIICVAIHICINKHRKRRRSYKAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWK7</accession>
    <name>A0A8S1NWK7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1000015</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8095950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWK7"/>
    <dbReference type="OMA" id="YYEDEIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="831"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="922"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1035"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1089"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1113"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1178"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="44"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="137"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="191"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="228"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="299"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="333"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="370"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="412"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="563"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="598"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8095950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1238" mass="142884" checksum="D93747FF76FE395C" modified="2022-10-12" version="1">MDQINVSFVQYHVQNVKIMLMINIQYRCECPFGYYQFDSQTCLPCTSPCQTCEQNQNHCLSCVDINQIITNTNLCVCNEGWILDTNSITCLKCQLPCFDCIDNIQKCITCQDQLHQQPDSCECDSGWIFDDNYFCMPCIEPCTTCEISTTKCLTCIDPHHILNDISQCVCQSTYYSNTLITCAKCQEPCFECDSNGCINCIDMNQILDSNKQCVCKPGYLQQGIVCLKCQNPCSTCINTINECLTCFDLNQIVKDYQCLCKEGFVKEGDFCCDQYCIDCQGVDNCNNCMKGFYLTSISRCLKCIDNCDVCQNQINCEICQEGYFLNEFRFCEKCLPHCKICGNQFNCDICLDGYFQLEQQCEQCNQNCLTCNEVSEKCLTCRNNYEINPNYQCICKVGYYEQQNQCYRCEYPCKKCLNQTICLECVMLSNLYLDLNFKCNCSPGYFWNQNNCSQCYQYCLNCIENQFNCISCDQKLNRILRNNKCVCIQNYFETEDGVCISCLNQLGKSQESCKYQDCKDQIWTFGEECDDGNYVNRDGCSNCKIDHNYSCSNEILNKSICFQCSTNCIKCQMNPQTKKSLCIKCKVGYFLDKNDCVECSINCLECIDQAYNCISCKFPQYKNHKCQICESGYYEDEINGICLNKCGDYIKVKEEECDDGNLIKGDGCDDQCKLENKYIFLNGVSIIPTYPKPILKSLGTSQIYSVIRLFKLSYTTQIVITDGFQIKNYLSFHIQNQGNAQKMDQSIQLNQETSHINEFNQSELILMINITFSRNSIDEILLIKFLNNSVIYSNEGYSQIETEVSCLIPKVIYIDDVTITQVQLATKSNTYVLYFIGAMCGGSVLFGGVEVFFNLLDTLQMLSYFKYINTQLPYNLQTYFELFGFAQFNFIQKIFDFSGFIDQILNTQQLKKIPTKVASDEITSLFIINSATITTVWISLFCIYGISKVIPKILYTFKFKFYSESQSENSWLVKVGVYYLTIKYVINRLCFTIISEFFYSGILRAYMATAYDFTFSLVLQLYALELNSSNIFIRFSSLLACVASAIYVFSIYFVIKLSQMKKYALNNRAIKNKYGSIFDGIKINEFSKYLNAILLIKKLIFMFLLIFAYEFPIFQTVSITLLSISISLFYILFNPLEDKLEYFKQLFSEVSISFTLLSITILTCDFQLLYFSYEIRQLIGWCCIFFMTSILCIQLGIDGFQQWKFLFKKYKKIKRLAQSIIGVFSKNKQVNGPSSIFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P176</accession>
    <name>A0A8S1P176_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1010064</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P176"/>
    <dbReference type="OMA" id="QTEKRIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002915">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="13"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="301"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8096383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="522" mass="61910" checksum="8294C053A3A57633" modified="2022-10-12" version="1">MATKYQQILDGKYGIVKVIGKGEFGEVYLAENIQTKSQVAIKQALKSELEKVKKRQELFQTEKRIMQTIKNENVISLIDHIETKDKHYLVMEYCNGGDLDSYIKLLREKNKQPLSERQAKEFLKQLLNGFKGLQDMFVIHRDLKLKNILVHDETIKIADFGLSKVGEVGFTQVGTPYTKAPEVFKGRGKIRYDNKADIWSLGIIFYQMLFDLQYPFVGQNEKDLFDNIEKAKDLNFTPAKTNVRISEDAKRLLRRMLTKDPNLRITWEQLYQDPYLEFGQQKDLGTFDQFYQNIMSKFQMIRELQLKIQETLGNSESLRKDYKVIKNLNQYYVQFMLAKRIIILSRKLEQLIQELKNQQKQQSKSLQEIGKETEENYYVYQLYYENTKFNLESFINRRKKEYKEYDEKVLNEIQNAKQMDWSYYTNIVLNYFYCLSNQAEIMEGQNETKALQFYDHMIDVLKCLENDAAFEGDKLKFKSQSNDEFESKQNNGPMMGEFTKEQYIQIQKMNVKELFQIMLSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P197</accession>
    <name>A0A8S1P197_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1010055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P197"/>
    <dbReference type="OMA" id="CDECFQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="7"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8096365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="126" mass="14621" checksum="E478B2BA641A59EE" modified="2022-10-12" version="1">MNKQQCCQQCESNENVLICVKCSQGLCDECFQQFGGKDFEALCPVCQMLSVFRQSQVEICEACADIHESEQEEMECLFKSMNLMKDKMNQLFLVGQQRIKRIEQLKQQIQINENEINMLKAQAQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1J3</accession>
    <name>A0A8S1P1J3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0960007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1J3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="338" mass="39724" checksum="EAB075F694B46675" modified="2022-10-12" version="1">MQEPKSQTNTQYKYFSYKLLSRLKINQHNCRALSIDDKKNLVFFSTQKGLQIYQLKNGMLKQLSQINSQKDVSVITTFMNRSLFLTGQYSSSIIVWSYNLMFKGKYLMKMKKHAYKISCLIIYPNPENLIISGSHDNTIKFWYLTPSSSFNCYQTIKEQECSVLALSLNQKGDTLISCDDYNLILIMNGSYTSKWQVKQKIKLKGERLTFLSNHSFAIQHRNSEFIFFYGYDYTSEQYVRINKLQIKGWRQHDRGQFFQYFIPSKQILLSKNGYTLNLIRFTLVSSSYCECYLEQSIEFQQNSEVGGVYGTMSKDGQFLITYDDESCEIQIRKYVEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5Q0</accession>
    <name>A0A8S1P5Q0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1060176</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035785453" evidence="4">
        <location>
            <begin position="18"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8098333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1326" mass="152596" checksum="357BD865B563FB95" modified="2022-10-12" version="1" fragment="single">MLFQFLYFQCLGTIVEAQYIQNLVTSQITNNEGWAIINPYNGATTHITDCGGNIIFGGYQVCGCISSRDTQLMKYFILPPHYQLSINVRHWKFNIILYQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQKIKFIQMSWSPQINETKLFQIKQVLFDNNPYGYNRNLRTATVQPTFRTIVEAQYIQNLVTSQITNNEGWAIINPYNGATTHITDCGGNIIFGGYQVCGCISSRDTQLMKYFILPPHYQLSINVRHWKFNIILYQELMIGQPLKIFIQFQIQRSRFGRLQQIQDLIYVVILVQNKVLQYVKNILCIHLIQLLQPFTHLVIPMYIYQLQNFKNFWGITDFSIELNECYNGCLFCLDQTTDCIFWIVWKSYFDLQNLNNGIEGWKINQFSRFQTTNNYFQIKFLVINKGLSAINYFLLPDHSSFIIQFCVDYPNSALSKFKIYLNDEELLEYTNDVKYVPYRSSIINDSRNQIKLEIQSINDRIAIRELKIILRGPINLISCIDDNMEPFDGCFAKQESCQQGCLFCVNGQCLMCDNKWIYDELNNNCEPLCGDKFIIVNEQCDDGNNIEFDGCYQCQFSCPLNCYNCLFGKCQECITGYYYSIGICNQINEYLFQGTDLLLRKNLNEYQISLAFSDMKFNQLHLYYQEKLIFQNGIETFESLCSPLCQVCQYNKCLKCLGNHLLLKNQCIPRITQGVFLIEDGIHLSEYLIGCNQCQESCQIQCLQCQNSYCILCIDGWQLLNGQCKQVCGDNQVAILSQEQCDSNLEDCLNCNIYCSENCQYCLNNKECLICNENYVEINHKCQLACSKDCKECIQGKCVDDILNGEINLDDTQDQICGDGIKQQSELCDDGNNIQFDGCYNCQYSCPLNCVNCIDGICYECEQQLQLKYNQCYEYCGNGKKNQDELCDDGNIIDADGCSSSCKIEVDYKCIENDNGLSECQYYESPQMKLKFLNQTFNKYYIDISFTQPIYFKNNYQLESLFDISIDDIKTEYYAIKLESNYQPTIDTVLNFQFLLEINLFISTNNSFAQSNIQLTNEAFNENEMKLLNPSLKMLLKQYIQMNKADIDKTKKLSEYQEVMIIGQGIICVISIMSGNX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6T6</accession>
    <name>A0A8S1P6T6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aspartyl aminopeptidase</fullName>
            <ecNumber evidence="4">3.4.11.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1080009</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate.</text>
            <dbReference type="EC" id="3.4.11.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.21" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6T6"/>
    <dbReference type="OMA" id="LLDTHYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05658">
        <property type="entry name" value="M18_DAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.250.10:FF:000001">
        <property type="entry name" value="Aspartyl aminopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002759">
        <property type="entry name" value="PRK02813.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570:SF3">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8098802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="471" mass="53214" checksum="1AEFB486D52589FF" modified="2022-10-12" version="1">MKQFPIQQARNLVNEFFEFVAKAVSPFHVTSLCKEKLTKSGFTELNETENWKLEKGGKYFFTRNLTTIVAFTVGQQFDPNNTGFKIIGAHTDSPCLRLAPVSKLDSNGFLQTCVSTYGGGLWHTWFDRELTLGGRIIFQKDNVFQSQLFHYKQPLLKIPNLAIHLTTDRNSFAPNNESNLRPVFAQEAYQTLTGIEKPKTEGQTSFENKHYKYLLNLITEQTGIPTSDILDIDLYFSDCQPPSYFGLNQEFISAARIDNLFSSFFALLAITNPESFTEDQTFINMICLYDHEEVGSQSAQGADSSLLSNNMKRIYDILSNSTQTSTDSFYKAIQKSFLISSDMAHSIHPNYSDKHQQNHRVKMNEGIVIKINHNQRYATDGVSSAILRVVAQSAEVPIQDFIVRNDSPCGSTIGPLQASNTGIKTIDIGAAQWGMHSIRETAGVVDGYYLEKLFVEFYRKYEKIDHKLLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7D7</accession>
    <name>A0A8S1P7D7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1080124</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8099012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7D7"/>
    <dbReference type="OMA" id="NAYCTRL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210:SF1">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-CH-type" evidence="5">
        <location>
            <begin position="232"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="4">
        <location>
            <begin position="361"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8099012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="454" mass="52588" checksum="E5D81F7A1AE57B7A" modified="2022-10-12" version="1">MGNCQNCLNNEQEQIEIVVNNPTVEDMCSMQEQSPLLVLDVRTWQKESYSLYDYENSSFIHCQQISINGNSYLTCKANKVLYNEGSYANNNENNDEALLLPLEFDNERFQLVNLRFNRRIQSKELIDEYQKEEGDKLLSENQTVHADFQMSFNIQKLRTNTNRQMVCEMNKGCKIWLVTRSIQNNSPNEGIILKVGDVIKLGRVKLIIKAIQLQNDQLQDTPDDISMCSVEDKNEDTKQCRICLSTGETLTNPLIDPCKCCGGTKYIHIKCLLKWIQQSSHFNSNAYCTRLIWKSLECEICKYQFPPIFERDGRTFNLIELSKPKQPYVMMEFQQKKQHDNTQNNDRNGVYIINFGTKKELRIGRNHEVEISIADISVSREHAHLKFVGDKVILSDKKSKFGTLVLLQKNITLQPQLSGLEIQIKRTLIKINNNQNTHSIQQQADFEQLLGRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8L3</accession>
    <name>A0A8S1P8L3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1100011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8L3"/>
    <dbReference type="OMA" id="NDKWNGH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="285" mass="33828" checksum="5B28D5A38AC06229" modified="2022-10-12" version="1">MGICKSTHIQNQEDLKILQAPILYVKKSPVLGIHRIYTDNLPQLNEFSHMDNQIRRASYRYNQNVNYNGQWIGNKRNGVGRLEWKDGTYYEGNWRDDELDGNGKFVSNNFVIEGQWKKNKLNGYGIYKVDDLYKYEGYWCEDYKLGIGKEYYDDDSQYIGNFDQNVKSGMGIYIWDDGSKYEGMFKQDSFNGIGELKWANGSWYKGEFMNNYLHGQGYYQWNDGRRYEGNYVYDYKNGYGKYFWEDNQKYEGYWKNDKWNGHGILNKSGCVIEGEWIQGKLQIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8Q1</accession>
    <name>A0A8S1P8Q1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1090165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8Q1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8099460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="12925" checksum="B2C4FFDB263A8730" modified="2022-10-12" version="1">MIIQTSLLMIIQKVVLRILQKPFIFKMQKQFFIKCHSKQQAELKLVMTIIQQIIKKSTDLDDQIHKDDVFLDEKLYQKKEAILNSLQVVLFQLRYIPQDFNSYYQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8R7</accession>
    <name>A0A8S1P8R7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1090141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8R7"/>
    <dbReference type="OMA" id="HFRNSND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="275" mass="31880" checksum="64DCB9ED44CF686C" modified="2022-10-12" version="1">MITGNLAELVWGSVELALNVLLYSRLKLPQKVMSQGLLRMSLVRECNYNFGVDTKIVRYVVASLLAPNIVRHFSKSNDSYHFMYGCFICFDALIVMDIFPMTDTIYAIILILRLFHLLEIIFKISKATQSIKIQMKKVIKIQYNSLNQLNTTSSYNQINTKKLEVSPPNSTQTTNASSLNEAESIQQIKKRPPFPNVYQIKIQTSKTTKINILIDEPDKFDLLFDQQLNIDRRMNLQIKLNLCAQVYYTLKKHYPCEWLLKQIAQYQAELLLLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBS7</accession>
    <name>A0A8S1PBS7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1110159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8100158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PBS7"/>
    <dbReference type="OMA" id="NSRMIRN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8100158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="26584" checksum="F1A51D6157F7FABC" modified="2022-10-12" version="1">MGTCSQGQNIQNVKGNERTLTKLVGYFYSIPMQQHPDIDSLIITKLERQKIISRGLSKNLIEEIVQFLNSRMIRNNEQDNLLDHFNYVYNQSFKKMNDFNTNLGKMILITTIEVLLCFKSLEMGELTPIDVWWVEDHFLWRYCQLQDQYKVDYDDFLDFNHLIKFLYLLKECIRIELKKMNFELKQLISSIKTEHQLQQQEIPNTARTVDTFRPQQKRLDTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PH75</accession>
    <name>A0A8S1PH75_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BEACH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1180177</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PH75"/>
    <dbReference type="OMA" id="NRIVICG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF112">
        <property type="entry name" value="BEACH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="2">
        <location>
            <begin position="108"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="1">
        <location>
            <begin position="209"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8102572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="817" mass="96281" checksum="A677449494047F02" modified="2022-10-12" version="1">MTQSEQLSKLTDDINQAAASMQVSVYKLMNKTRKIRQRWCKLWHNLRITLYRPTNCKFQVDEYIDHDYKFNENPYYINKIEKYINKYYSRPILKIKFKKNTEFENDIQVPLEKKQQYKCFWLKSLYLKQGGVTIGDDKITFSYYSYTQQANHITLLPHTIPNHKPFKKSWKRNQIRWLYERIYIRNRNAIEIVFKDGESLYLIFQDEGLKEEIIEKLKIQPKQCDFNKVSNFKYLMYLNFLSCRSYIDLTRYPVFPWVLKGNASDFDDNDIASIQNEINYRDLSLPIGACGSESRLDQFIKRYENEQFYYGTHYSSQALVSQYLVRLSPFTEAAISIQDGKFDIPDRLFRSIMKSWIDCNNEMADVRELTPEFFYLPEAFLNINGYNLGKLQSSQVVNNVQLPFYSNKNPFYYVAQNMIALESEYAIKLNEWIDLIWGYKQQGNAAIDSFNVFYPLTYSNFDEGSIEINELAMETQIAHFGQTPEQIFYKPHPQKNNEFDKTQWQIVKNKKSQNKLIYLICNKNKVISIQEDFTLIQWQIVKNQQQFQIDKQVQLPKVNFDAMIKYVLPPILVLIEQSIILFGGNNNGTLTYYCLDNLKLQGVVELESEIINNYCTITVLESNKKQNIVILGTNKGLVYYYNKVLQNKQDIKQEEVLKGKNVIYDTTSQINSITISEQLTIFCVGTQDGTIFLYNLYNRTLYRFINHPKRLPIYYLTLSYSPLPCILFSSHDERVVAYSINGFLLQFLQVKGNIEYIQIKKSSINTLIVITDNNVFEYRLPFLDRLSVVSSQTCTRFVQATKQISIYGCKSGDLRII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PI19</accession>
    <name>A0A8S1PI19_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1190124</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PI19"/>
    <dbReference type="OMA" id="ENETLQC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000509">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF702">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="3">
        <location>
            <begin position="3"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1106"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="513"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1106"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1138"/>
            <end position="1150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8102865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1156" mass="136906" checksum="D2AC32B020B9C22F" modified="2022-10-12" version="1">MYCGLNNLGNTCYMNSLLQTLFMTRQFREALFKWKYDETKHPKREDCIPYQLQYLFTKMQEGYENRATDARGLVRSFQWDPQESFAQHDVQEFCRVLFDAIQQSDDQDFITRLYQSHSQSYVQCLKCQQESVSNEVFLDLSLTVKSDFLNVYNESLERALYYYIKPEKLEGENKYYCQNCNEKTDALKGFRLKALSDILTIQLNRFELDMNTFERKKVNDYVSFPFVLDMNNFLKPYDQIVIEDHPDLLEEREKLKLQYEQEKLQGCFSDPLRRKNRKPSPDERVSKVHFNDTPQIVQPDDKLQFKKQLLEAHQEFQQKFQSLKSTNTSQTQFTSQTIPNNPNLILTADEAYDFDNDILFGGNLDNLGDLGEGTTANTVKTIKEVQGSAKTQNYDEKEIQERIENESHNLSIKQKIQELLQNGPNVYELYSVMIHSGGALGGHYYAYIKSFENGDWYCFNDSTVSRISWKTILTMFGDKYPKYNMGSSAYFLMYRRAGEELSTQKPTIQEHLKEAIVKELQEQKEREEKWEREKKEREMELKIRCFYKNNDAVIKTRKDTLLKDFKLQVRQELNVQEDEENTRIRFYNHMDNIPQDTFTNKENETLQSLYFNDFKSVLLETKTPHEKFEDYDPHLMLIKLCVWRNDIESLDESELKPLKIFIRDDKTVEQFMDTITQRLGVPKDKQIILQKNWINGATNYSIRMNQQHQLDFTLYDLKIGRNVTLYLEEKQDDIDLPLNWDQKFECEKHKCRVTFNDPRKQLLPLESPDYCFELQIDNRKTMQELKERMSDFLGIDIDSFIVKKSNRYGEEIKDLTTTIKNNQAIGRAAFCLMMGKPSTKGIYKIKVGMGGLSPKYSDTIEFEYQDLFDMEINSEWYVKELKEKLCQKLEELKQITLQPNLIRLRYKLNTKLSQVYHDDQLLKNLRLFDNQTIIIEKLEKQDEIKENQLLGYVRFYNPKDFTLSFPPKEIFLNSTDTMHEASNVISNLFNINQDAIEAIKINNIFSFTITDLVTASFDWQLLKFNQNTVSKSPWFLLKDSYFIIVRDAREVPKDMTELRQKVDEILAKSSDPSKIQQKIPVQSNKPKEKALKITVVKKQDEQLKQEELQKQQEELQKQQDELQKQQDEQQDKVEQLDNQDGIQQNINEISQTEKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJC6</accession>
    <name>A0A8S1PJC6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase C39-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1200004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJC6"/>
    <dbReference type="OMA" id="DSAWAND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039564">
        <property type="entry name" value="Peptidase_C39-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40524:SF1">
        <property type="entry name" value="PEPTIDASE C39-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40524">
        <property type="entry name" value="PEPTIDASE_C39_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13529">
        <property type="entry name" value="Peptidase_C39_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035833298" description="Peptidase C39-like domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C39-like" evidence="2">
        <location>
            <begin position="19"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8103044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="177" mass="19638" checksum="E43EDDF63597D580" modified="2022-10-12" version="1">MNKIIAILFVLCIANARQYTLYKQCDSAWANDQLGTSANTICRAGCLISSIAMMLHTYGVVTDGTTTPKTMNTWLRKNGGYASGDLFVWASINPLGFVFQGWITTTQAKYKFDAGLHVILNVNNGGHYVLMTSYSGDTFNVNDPGYSRSTYSANEVKEAAYYVHSKITYFKNHVLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKM0</accession>
    <name>A0A8S1PKM0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1200043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKM0"/>
    <dbReference type="OMA" id="FASMDIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029147">
        <property type="entry name" value="CFAP77"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14825">
        <property type="entry name" value="CFAP77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="203" mass="23321" checksum="A27F21710C8B0020" modified="2022-10-12" version="1">MSNKTVYPWQKYSANPLIVKDDVGKAKPNTYPIPDCAFGTRTVPGKEETLGQVLCHMEHFASMDIRPEKDFKKTNIMSIKRNLCTAKEFQEFRQSHDIRQKIVQGKPKIIETPKQQVFGRKNRTPSPIKNVLAYDYGTVAEKKQAEAYTNRPSTAKSRISVETKSSKLLKETIKKNQLIQSQPEQLKKLSQFTNIQAKTKTRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLC6</accession>
    <name>A0A8S1PLC6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1210131</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLC6"/>
    <dbReference type="OMA" id="KYLGCNP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106335">
        <property type="term" value="F:tRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000195">
        <property type="entry name" value="Methyltransferase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051422">
        <property type="entry name" value="AlkB_tRNA_MeTrf/Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069:SF21">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="5">
        <location>
            <begin position="231"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8103604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="634" mass="74070" checksum="EB3309A2044C43E0" modified="2022-10-12" version="1">MDLDIAIETKEKVRNPTIKLNKKLAIAIAALTKSEKKPFDELVSQEPTNKLIAVHFGVGDQDDYNTICNYVKQFGQNKIMIYPVRNYAFIEFQNIQQSLQLFQSLQVFENIKYANLQYPNKERATMFFYTNQQDLTGGGMCDIPNAMRQVNIPGLYLIHDFITPEYEKYILDLIEKQEWSKLKQRRVQHYGYEFIYGDNTVNVNQPADKNIPAFLEDVRAKVSELIKPQDQINQLTINEYLPGMGIPPHFDVHPPFHEKFVSISLLSGLVMSFKSFKGEEQHLYLPPRSCALFTGEVRYAWFHSIAARKIDKVEGETHFRSRRLSLTFRTIRNDLKCDCEYQFFCESQGYNPLTMKNKNPLLQEYLAKLNQIGIIQDNKVVPNKTQEEIQLLEEQPQQLQQMPKATEVEKKYVYEIYEKIAPHFSSTRYKPWPKIEQFLKSLESGSLVADVGCGNGKYLGSNPDIEMIGTDRSENLLKICKEKSDAYQVFSADSLRLPLKSEMFDAVISIAVIHHFSNKILRQQAIKELLRICKSKGLVLIYVWAMEQEEKTFNEQDVFVPWNLQFKYEDEKVINQEVQQQFKIDDQKKTVVYKRYYHVFKQGEIEELLSEMPGFKIVNNYYDHANWVVVLQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PS48</accession>
    <name>A0A8S1PS48_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1270095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PS48"/>
    <dbReference type="OMA" id="GNNLCFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="25312" checksum="3B8E959EC0D87D41" modified="2022-10-12" version="1">MGNNLCFQTGERSSQFPQLLLKYEIGNCEIALTAGPVENEDVECLVIPTDSGYTNIKQSDRPSFKQINEKCVFVAGSKKRKSQIFAVVMDKNYSVKNDDGEDEKQTIYATVQEALRLAEQKQMKSVGFPVFESKDHTTSATIMLMAIKLTITEQKIKSLKRIAITLDHIEQVSSFKWVFQQVFKGSEQLRYSRAYSSSINTQIDSISIDDLKSKVKSNTKEETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PS65</accession>
    <name>A0A8S1PS65_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1280132</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PS65"/>
    <dbReference type="OMA" id="IMKANTQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="97"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="448"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="467"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8105834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="485" mass="56698" checksum="1521280B2DB722A0" modified="2022-10-12" version="1">MSCLRKHLLKSKTYYLEVLQDQMILSEHKTMQNPKYVVQFNFESRVMWKKSEHEQLCCFGILYQKRIKWFWGDHKELNLLKMYLSNRVFFGNISIFYESSQIIGSGASSKVCLVKRNTDVLYYAAKCISKKYLTGKKSNDRIARLQSEIEILQKIDNPIFVKLLEIYEGDNSYYLITDYYEGDTLYNFIRNTQSDTLPSYIVRDGIKTLLLGLQYLESLGLIHRDIKLENILLAKQNEIKSLKIIDFGLAIYENLCTKLSVCGTPGYIAPEILRVENQQNEYFTTKCDVFSAGVIFYKLLTKRTLFRAENTADIMKANTQCDIHLDELQLNVQKEALNLLKLMLEPNPKLRPTASQCLEHPYFYCQLEDIRIIQEILDKVTGCSEFTKEQHQDTHSVPNESQAAQIKRCPPTQYRVRIEQRKRTRSPRKYADFQFYLPSFCQMTSFESEGNSSNSSKSSRIENIIINEKESEKDSVIEENNKFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSS7</accession>
    <name>A0A8S1PSS7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1290139</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSS7"/>
    <dbReference type="OMA" id="YDNAINT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="204"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="272"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="306"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8106176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="45548" checksum="CD29DA0DB90D4159" modified="2022-10-12" version="1">MGQAGNQKLKCDKDDHENEIDSVCYNQFCTEFRLNCFTFIRKGIHHSHLDDVEKSNSLIEYIEKQNKESDNLINFLQKLVENLNESFSLFKKGISYKYSLSKERLVNLNSQQINDFLNSTIKFAEYKESIINIISDQIVKLTNNLNNLYDQLNIVSFNYYQISSNDIKLSKQIYAKCQDLIQENKFKESIQLLDKSIQLDLNNYQALWCKGSCLKDLGRYEDAIIWFEKALTINPQHVNSLWDKGVCLRMLNRYEDAIIWLDKVLVINPKDINSLFYKGSCLQMLGRYEDSIIWLDKALAINPKDVNSLFYKGTCLRKMKRFNDSLKYLDQALSINPNHAFSLGAKGQLLEDQQKYEEAVLLYEKSLKKQSDDQWTINRKAFCENKLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXC3</accession>
    <name>A0A8S1PXC3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T1350085</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXC3"/>
    <dbReference type="OMA" id="HAIFAIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000029">
        <property type="entry name" value="Kinesin-like protein KIF17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF21">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="5"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="744"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="412"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="744"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="89"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8107920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="793" mass="92059" checksum="DBFDCCE9851CF20E" modified="2022-10-12" version="1">MPAECVKVIVRMRPFNQREKENGSKPCVLVNEDTNSLELRNSQDNELKNFTYDYVFGAETPQLQIYQKTAFNLVESVADGYNGTIFAYGQTGCGKTFTMIGDPTNENMKGIIPRTFDQIISLINNNSDSNKKFLLRCSYIEIYNEEIHDLLSKDVKQKYELKEGQQGVFIKDLNIVVVRTTQEMDKYMQIGTQNRSVGATAMNKESSRSHCIFTVYIECSLTDSKGNERITAGKLNLVDLAGSERQSKTQATGDRLKEATKINLSLSALGNVISALVDGKTSHIPYRDSKLTRLLQDSLGGNTKTIMIAAISPSDFNYDETMSSLRYASRAKMIKNQPKVNEDPKDALLKEQAEEIKKLKELLSRQAAGQPISIESLIAKPENNSNPEISRLKEENDRLLREKQGLPSAEHSEEKLKELNQFKEKNNQLQQEKEKFEQEIKEKELKVEQERQVRQRLEQLLKEKEQMMVQGGKGSEDDKKKYKKLRQTVDQQKKEHEALIQQQEQQQQEMLEIETKYQNVQEEVEKLRKLIKYLRKKLEEATIEQKDLKLEVENEKEDMLETIRNQQKEIKLYISLVKMMFNQKELETLQAASEWDEDAQEYKIPPFNFKAKKVNFPALPYKQAMDLIELEKSERVLEVNSRQQFFEYEQDNRLRLVSPKIPRKESQQKQNGISQPQLDKGYLVAEKIKQNYQLLEQQEGKQRYQSDQVNQQFIEKKLNQKIILSPIDNRDNSVQSQHSQYNYNGYSNNSNLSQATPNKNNKNINLQPLEQKALLIQNDDLHKKRQHQKVQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PY82</accession>
    <name>A0A8S1PY82_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1360042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PY82"/>
    <dbReference type="OMA" id="NDECQQV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="133" mass="15791" checksum="979A95C3DE82C713" modified="2022-10-12" version="1">MQNLHLPTSQLYYEICALSRNKRINDIERRALKKLVILDDKYIFELIDDYNQKRITETKLQDQLILLSKDVDMKSDTNDECQQVLDYQFNHAEAKKVIDFIQQNPQIPEEIASPLGAQLWKKRRAERQSRKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYD2</accession>
    <name>A0A8S1PYD2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1360014</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYD2"/>
    <dbReference type="OMA" id="ANWGYRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051242">
        <property type="entry name" value="WD-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324:SF4">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="71"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="171"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="271"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="313"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="358"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8108111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="975" mass="113206" checksum="7FC711A9D1B1FBCD" modified="2022-10-12" version="1">MKNQDYEDYDQDYEKKEIDIMEKLHKKKMAVIERCFPDKNSKGQGLTIQQFLKVMLEHLDYNKDSKEETKKITLALIELFKEIDVNGDGTMEWVEFSNHIIELGLLRNDRSFKDVIKSYHPAENIKDEQKHETVIDRVYFFDRLKMLLVLEKESPKFKVYNSNTSELIWNVNAHKGYVLSAEFIPDQNLIASSSNDLTINFWDSSSFNLKQILSVPEIQLCMRYARWSSSQSNFLYTGGSDSIIHIYDTYDLKERGTLSGWNPFIKRDSQQYGHSSPIGDILAIDQQNTLVTGGLDGNICLWDSATHQPKKELRGHEKGVYSLDWSDWSPMNQCLISAGLDHEAFVWNTYVKEKIFLLRGHNHPLVGVKCLPRTSQVVTADISGMVKVWDVRNFLQIQTFNVPADEIQAFTLTYPKKQIVVGARKMFFYEYDEPKDQMLTDEKMCLKVLYNQTLTCFITLHPDSVKTWDARTGKLQQVYRELSVAELTTMIIDARQRKLFIGDSEGRIFTVNIKNGAKMKKFERHHKMITDLAHWSNDTNRRVISCSREDTVNIHDEDSQDAHKSCRYKMKQHHLSVNSLHVKQDSENLVSCADDGAIFIIDLVSYRQELVWKCQYELKKVMFLESHNCIVSVDSIGNVYFIGVLESKFKSKLLLQKTYKAISLTNQEETFPVTSINYHDNLLYLGDELGNLKIWNIKQVLDKVDLHQVEQKIKTRKNNDADTFVTAMDYGQEDIAQIFVIGDIMEVGYQRKAHQDGITYIEVCKDGSHFATSSFDCCCYLWAFKNGQVSKIGALILGHDANWGYRIDEISRAEAAEQEANELLEELKDTIIPTKVEKNDGQISSSKQQLLDRLYGSKQQKDQQLQQNQAEKEDKKEMALNRAKNALKIYEQFKQKQFTYQNQQNQNDQDIKKYDDSEYKDVFQQQDYDEEEEDNYNILTDLQEFKQQQLSSGVYSSKNAFKTKLIKGHHKSKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZH9</accession>
    <name>A0A8S1PZH9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1380068</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZH9"/>
    <dbReference type="OMA" id="RSEREWW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000560">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF379">
        <property type="entry name" value="CALPAIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="90"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="185"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="276"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="364"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Calpain catalytic" evidence="8">
        <location>
            <begin position="483"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="4"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="553"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="724"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8108807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1007" mass="118468" checksum="297CACD81CB7F8B8" modified="2022-10-12" version="1">MKSIDEYKKIVITLKKKVKLLQQEQQDLKRLQQEKVYVYSDRYTAIKQLKKAAKKLGLTTEMFFRAADTKSMEIVTTKRLGEILKKVARLHENYVQELLNIFDVENSGSITKDEYYQTLEMYQINEEYGNNFNRNSYSKFIYFLKREQYDGIIGFIKMDPNKLGYITREMFVAFTQSLNNWQQYLTLREIYSAFQYMDISENDQISREEYLQAFEKLPALPQIQQIQEQKVNILRKVLLIKGVKLSSFVLELMLSIQKPELGITIEVFYNVAKQYLDRKETLAFFKLMDHQQKGSIDYDEIMSFYYMTMSYGDSFEIVFTILARKLQAMNKSIEKYLKQEQIYAETQLTLDQFNQIAQDLFNTPNYEISAKLFEAMDLDNSGIVSAQEIITYTNESLKNYSLLLRQKTQTQTTMLFEDFDSTQIEIANLDIEEQLLFELKKYKTTFSDKDRFENFQYIFTNEISCLVVCLQIVENLKLNKQKYWEDPEFGSNKLDPYGANSIFRIENLPVKGWPDPKDLCWQRISEICNDWVFMDENGANANDVIQSHYLGDCWLISALTIIACNDQYLIDQNDISKGLYPKLFHFLSKYGIIVIKFNKMFRDVYVVIDDRFCTYNNKLLFAECRSEREWWVQIIEKAYAKLHTCYESLTSGDISQALFDCTGIPCTRYVIDENDHTKDPQEKKQQLLNLFQVGKQTNALMGCSAHGTGQVLLNGEETGLYRGHAYSILHYFHLKDAQSRIHKLIVLRNPWGFGEWKLKWSDYSIEMSENFQLIQAYFNEEIQRCEQNGIEPPEQYNLGDDGIFIMNFKSFLNIFNNLFMSVNLDEKWSVMRIYDSFTKEHLGTPNKNERSQAQYPYNNPRYTIRINHPFPNKKIYIALSQKDGRLDRFAKYPYEGKLVKIIMCLFQLQQNETELTKFQKPVLDSKLLSGRRDIDMEVFSVQNATYVLIPSAHKPNITGDYVISVFFDFPPNGIIIESSTEKQFLLQPKIFSSLMQNPTLLKLFNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2L3</accession>
    <name>A0A8S1Q2L3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1390075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8109079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2L3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="150" mass="17705" checksum="387E89E8CED77BA7" modified="2022-10-12" version="1">MSYQLKGLNAKRLQQINQSTFKTRKSQDYPCDLSYEYQPRNNSFKQTRAITQHNQRLINEDEISKMVRPQTSEGTRRMRIKEKHTEIGNLNSNLKCDILECENEEDNQEQQLEINYHIICNSSNTTRSLKPKIPFQTTLDQDFISLFAND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q669</accession>
    <name>A0A8S1Q669_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1450085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q669"/>
    <dbReference type="OMA" id="MIIQPRY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="879" mass="104107" checksum="7FB045236680F177" modified="2022-10-12" version="1">MIIQPRYSQKMADTFSNSKDFYSFHQKQFMASKPQAQRLEHLSISQRTKIQSSKDRTSSEILTAFINRGMLSTQQKVSTTKPYDNSSSILIKQSYDEKIIREELLTQQALQTIEDFEKSKKILPPLRLYADKLKLKERQNQSKEKVETFKSSLIETPTIQRTIVRKNRGFIQGEQKSTYENEQSIQKMGRTSSTPLMQKSKEDKEEQATSRSGTPQKETSNVSPIKNPQKNLTKQLSIYTPQQKTIKDKKLHSASHLSQPQQQQQQQQQQYPIQIDKKIIPRKKKKQNQVNIIQQQNIQKEKLNLRHTSYRCFLNKINFSNNQCISCPNKLEVPAYYYYVGLGNNGSLVKNIFRQRWWWSEVETLDTIKVNMSWTQLKQNICIEQLHPYNMDSGTQSSDSVILSYEETIGDTSDSDIDVRPKQLSITGGIHKQQSSSQHQQKKPPNYYSMKRILNAQDLQKLVNYMEEINCFDSQLIFSDCSEKLLKDIKQYSTIQRLDSRNQKMHNHMEDNWHLGNKKALFYNMRHYFQIIKEDYNKLLPVTFHVQKGLSDIEYSRFLDYYNKRNEELREIEKNRDRKDKKKLPLNLWIIKPGELTNRGNGITVCNDLNEINKIISEEIQEGRQRTYIVQQYIDNPFLYNKRKFDIRCYMLLTSQNGIFKGYWYQEGYIRTSSKEFTTKCLDRFVHLTNDAVQSKDQDYGKHEPGNKISYLEFQRYVECNYPNSKFNFFIDIYPKMRSAALDMMKATYGKIDPHRRINSFELYGLDFMIDENFKLWLIEANTNPCLEQSCPLLSRIIPTMVENLFRIAVDPIFPPPFFEEWPQNKKLFIPDNVIENNKFELIFDELIEKKNMLSLFRDNKIEQECFDIEEEEEEEEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QC64</accession>
    <name>A0A8S1QC64_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1540037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QC64"/>
    <dbReference type="OMA" id="HQKACEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="91" mass="10662" checksum="6DEF415AFAF7A31B" modified="2022-10-12" version="1">MNAFSLEKNRFHQKACEKKKKRQSENFNSQTKNQQIDNYIQQKTQSLYVDLDSSYTKASDQDSFKLNNIPKSVRANLIGQLNNKYGRKSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF97</accession>
    <name>A0A8S1QF97_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing]</fullName>
            <ecNumber evidence="3">1.3.1.124</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1550059</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a (2E,4E)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:45912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85101"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2E,4Z)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:61892"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85099"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.124" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF97"/>
    <dbReference type="OMA" id="ITPMGRP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008670">
        <property type="term" value="F:2,4-dienoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05369">
        <property type="entry name" value="TER_DECR_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045017">
        <property type="entry name" value="DECR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296:SF2">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE [(3E)-ENOYL-COA-PRODUCING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8113437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="280" mass="30424" checksum="586965FB0D34A647" modified="2022-10-12" version="1">MNKTFQQNFLANQVAIVTGGATGICYGISLAYLKYGCKVLITSRKEEVLKQSCITLAQESGNNNIAYFPCDVRKFEQVEAMVQFALEKWGRIDILVNGAAGNFLVPFEMMSVNAFRSVMEIDTFGTFHCCKAVVAKWMSKNGGVIINISTTLPHCGVALQSHAGTAKAGIDALTRHLAVELGPKKIRVVGIAPGAIEKSEGFKRLRMDDSSGFGEDFEKLLPLQRAGTNDDIAPWALFLASECASYITGQTIIVDGGAVNTFPNFTLLSKKARDMLQPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QLR9</accession>
    <name>A0A8S1QLR9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1760033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000185">
        <property type="protein sequence ID" value="CAD8116673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QLR9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="85" mass="10450" checksum="A368ACF79667437B" modified="2022-10-12" version="1">MQVIKNETVQQLVHRSQYNKIVWQSSNPYNKRRQYKFKNAYYTQRYQLRAYICKDIKRQLGISTTWMGMDLRFIAQTCPKFLMFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QVQ4</accession>
    <name>A0A8S1QVQ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T2710002</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000280">
        <property type="protein sequence ID" value="CAD8118904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF91">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PLK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="700"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="867"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="963"/>
            <end position="1014"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8118904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1165" mass="137077" checksum="E5F90935EC441337" modified="2022-10-12" version="1">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</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JML9</accession>
    <name>A0A8S1JML9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0040210</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ANEFGQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF4">
        <property type="entry name" value="RYR_IP3R HOMOLOGY ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="2734"/>
            <end position="2753"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="2787"/>
            <end position="2810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="2877"/>
            <end position="2903"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="2924"/>
            <end position="2944"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="2956"/>
            <end position="2977"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="2998"/>
            <end position="3021"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol 1,4,5-trisphosphate/ryanodine receptor" evidence="9">
        <location>
            <begin position="44"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="RyR/IP3R Homology associated" evidence="8">
        <location>
            <begin position="2288"/>
            <end position="2391"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="2866"/>
            <end position="3031"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="2064"/>
            <end position="2091"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8042991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3131" mass="370021" checksum="29544CBCDBCBC31D" modified="2022-10-12" version="1">MDLPPIPIQKNQSQLGVDNLLSQQHIFNPLNQNQETAKQPKRNFNLKVGDIVYFKSYLTDDFKGVISGDGIASNKLECIQILGKNQQLTDPSKKSLQQKVGSLTFQKSLFQVVTGKKYQYQNFLVQERSRSIDDIREEAINESIDLDQCQLDYESRLKELEDKANEEIVENIRFYDLAYGSNIVYGQEIQLKHIYSQCILSFNCAILAKENCCRELSLEEIPNINSNFRILSMNQVKNPGEPILYGDQIIIQNSSSNKWFLNIQKPTQKYDKKDGLEVNCSQVGYPIKIASYIDYKTEQDLKQQSLKQKSLLSGDVIKIKNRYLGGYLSIKRQLRITEGLDKKEVFIKNYDTNITFTKHQFYFNVQQVRNEKIEYMYQLCVDSSADAEENLNNLWQIQHVDSLTFSKANYDSVFLIRHVCTGLFLEISHHDAGLTYDGLRQECQFYLRSKKTSDDQILFSEAYKLQSVMNVQIDNFVTQENLVIVCLDDRVTVQRKSQKQNIERVTFLLKLATPEQAKTAFRINSLQEYLIQFYIFLQDWGVIKTSTQGLEDIRTYDYFEAFNNQKILYDEILQLFQTLENLKMYLTNEGQAQTNEQQQFKQKALMDNDIINLLFAIQRLCNFMIYGSMINSNSQVVDKTPQKIAKMKLDPAISQSQKLNCIQEIYNVLSLCVQRNSDTSNYVLTLIMNKESILDFLLEQLKYQRKHISNLIKESVRYTDMSESKDNIKKWVNQLENLSEDNIEDQTLFIEILSLIMIDPYENPNQLCQNTCRRLLFGTKNKQEQQYPFHKVLVALDIQEENNNYYPIVQFYPKREKGLQMQYANEFGQNNQIFCQLYLKFIDKQARIAQRFLTKRPSLIDVVIDFFTLETLKENQLVKQEDVKLVVPIFQKYENYLMNVMTLYSSLCKGRNQKNIKCLMRNCFLNEKFLEICLKRQQNVKSELRFEKTLIELFCNLYLDIDPLIKISQNYDPFYNNTQTNTIKCYLSDDIEQFNIQNPSGLYFYENNASKSLKRSDDYQSFLHQKETKAEHQQLKLYAAKMLSRTQVNDVRLYYLEMFTKEDYPEHLLQEASYDIFQSKKATINKQKYDYSRLAYLQLHYFLGLLTIIKNSINLGYNQLDENQRIFSVLPNIFVALIIQQIPDLRIHKFQENESFLQNLNNLIQSVNDNLWVVTFLPTAEQLYEKKKSKYKNLDKDLKRMDQSQKLYMQFQRNWILYMLIWTYQYCNDEILKMQVYLEALSILKIFEQLKLNLQILEFLFSSSQQNESNPSSPSQRDLDLFVYDEVVNQEVDIKKQKTPAVSKSILSVFKEPDGNSICGLLFTCLLTSEKRTKLNEELLQRIIQSFLQPKYSIQEINQVEIIDNQQELRYFSIINGNSNSVQLISPKEAIQLSKRALKFITNQKENNVIQKRVASFDQLKAYSAKIIEQFEMFFKPNSQDLQYLKSFQNIVRNASVHLVFLKFLVDPQIINCSNDIVSFYKRLVLILEYFIIDNQVNVSILADNEYLLKILDVIQIQQPQADNKFYIPIKITKLAIKLISSIPENEHDNLINKIFERIQKIGDQLMQSQEFYLKAYSTRDEQEEIKFIQRSTHINAIAYYSLIQYFKILRSLTKSFEKPSKQAPLNKHLILSKILKNKFLRIVLDPVNYYKAILVPELDSGEEEVKDPLFYHRMKLHSELIVLLTECCQYYRLGIQEMQRILLYEQLKTILLAPNGEYIVKRAYLQCLFELYINQVKEGQFVNDTVESDEVRDILSRVIIPDLDQKQICKYLEGLSKLANQDRNMKVIQKDLRESIKRQKLTYFEKKLSFDSQFVKELGILRDSSEYWKYLRKNGIIHFLVYTYDEMKDRTDLENPENSTLSDEFAIIKQNVQKIKEIFNVLERDFRVKKEDLDLDDYRELITSIEEIIPQRKITKFGQNFRIGFQQDKNVNKLVFDQFDTLKEDQTGEKKLQEQENVQRIENPFRRNFKVYLIRYKVNINQFNEYIENNSTEQERSQKILLTCNTYKTYIQQSDIDEIYKVNVEQIKRQEQVNNRSKALLDWDLFKQAMRDLFEMKSIQTVINQQVQTYQRQQQDLENLKQEFEITCRKQIGKQYKKYGGQLEGSEDLISDDIQIELLIKTLEKQNTNLLNEDNVQEFLQKCQGIFLNREFYLIKLCKIFLFLKRPTQDDIDSLTEAKDEKSKELIRQKQQIFIKYQNAIVDSNLDILALEMLNDSADDLHKIEALQLLIYLLDYGNDYVQKKFYQILKQDQVIKQKFFVFLRGFFLIDVNSRILELELCEGNNTEYKILCLKVLKLLQAMCENVNVDFQKFLVRQYDDDSYQANINIVNEVASLLADLLEKGQRVFYKLQEIYRQALESLVEFSTGFEENKKELCKNTRLFTLLNQILQKEDLLSFKQLHQENRNIIKDNLLKSQNQEEDLIINLNKNQADVQQRKYNSYQTLQSFIKLLLLITQGKLDKKCLKFILETVNITILMRISRSIYQDRIKPKQRNIILDNICDESKTSQHKYCSNNLCHFGLRTDEDNLLIQTGFNIFIICLKLSEHFPKDPQLEIFIFDEQEEDQDDFVDLSDPLQEDLQKKNTLPTKKIVPIFINNKNLDQEEQNALLSNELRQTNQNEFKQMIDDIQNDENYKFTRKQQFFKFYRQFTGRIEIQNELSELEKIFFQKPFVCNFVTPNIKQHLIYEINRETDEDRMQGLIDQADFYQVQMRHSQQINNSFIMHFGAVYWRLLKDLSFILCLVIVILLIFMHDIVVNSKLGSNQEAPESDTKIPGQNFVSYLNNIITIIQLVLNLIIVFFCAIERYPISITYNRGETNAKRVQILKKEAGFSITFLTMKYYTIIGYFESEFQGQKVSESILKKLILVTFFDFDNFYNVCIFGLTVYAFFNPYIYAVLLLDIIKRSEDLQNIIKSITSNGRNLAIFSFLGFIGLLIYAIIAFSNFDWMFNDEDGVYGQTFILAVTSTINFGLRSGLGDSMKIYPEPYEDPTLYWGRYFFDFSFFIIFNILFIQIIFGIILDTFGELRDERQALVKEIEGKCFVCSLDKNDIDTNGTKGWHYHIYLEHSVYHMLYYIIYIKNKDPNDCNALEKYVKKCIEGKETTFFPFERALQIEDQKNEEEETIEQSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN92</accession>
    <name>A0A8S1JN92_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JN92"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="1">
        <location>
            <begin position="34"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="285" mass="32756" checksum="27199617CF2BD748" modified="2022-10-12" version="1">MLQKVPSQISLGVGSYSSKRFDDKLKIKNGDFKIAGFKIYISNNKICGIGIIYQNKANGRKIIFTDTSQNQKYSALYEFKIPSNDYIQVIFGYCDDDQLNQLGIITYSGKQEIFGIDKGTKFNYMFMGYTFSGCSGIFRQGSIESLNFQVLKLPKDYVFHNLSPLFDILYPNILEENTQNSEQSDQNTLNLSELSDQSVENASEIIDYQSTNQEQISQSNDIKKKEYSEFDLQQVNVERRPQQQQFIYGPNYNRNPQRNGFDPSVRQIRAGIIIGEIIVKLILRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQP5</accession>
    <name>A0A8S1JQP5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0060001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8043919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQP5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="164" mass="20209" checksum="94DF22D4A4934DE8" modified="2022-10-12" version="1">MKMDLNRKECKKIFKLQQNLNQVLKYQHYFKFQLSINFIKKYYSYDSQIKENLYINQYFQNNSQQSNQLQQILEINQKKSKRSQNKSKTSNLNYKKQKCYSKWLCINKLRLILRSSENLRQFIVSVKYNKQDIYVERTIIKFNSFTFSLNYIYQKRLQEGIKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSQ7</accession>
    <name>A0A8S1JSQ7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0090110</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSQ7"/>
    <dbReference type="OMA" id="TIFFRYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="5">
        <location>
            <begin position="59"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="6">
        <location>
            <begin position="336"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8045085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="499" mass="58938" checksum="BB9F07B2C58F92D2" modified="2022-10-12" version="1">MFKKVKKALKFIKPKKQKPQNDQDFELKRKTTVIQYDEMLKTKFNEESTIDTSKNKEYEEYEVQLEDSLIGIALKFDMSVELLKRMNNLSSEFICKGMVLKVFKVNQEDSEEEDQDDEEEEVEQIQQDIWKQQIQQFNNIPMYYCMDDQNVKGQITVREDMILFDTNNIQQQAGKRIINNACISINDINEAIVHILPNANDQKDYLIQILLSGIGRVEFEKKFRKKLEKLKKQKKSIATIFFRYQERYESGQLIPEEVKKEMCKTITDFINHSVQNLKEQDNVINLTKLPYIDLIDESNRYQQLIQKQELDNVIGKRMGRLWASISYVPKYKANSKCLNESLYKQLIELIPAIYRLSNWNIKFTIDNDGSSYQQLLLSLQYQNPFFFTIKDKDGNVFGAYVSSELKNQKPGFHGTGETFLFQQDQLQMIPYYWKETNNDFVYCDSDGFSIGCGPSFGIFVDSKLYFGYNNPCTTFENPRFTQNEKFFIQHFEVWVIEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JUL3</accession>
    <name>A0A8S1JUL3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spindle assembly abnormal protein 6 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0090305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JUL3"/>
    <dbReference type="OMA" id="DQMSNKP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10142">
        <property type="entry name" value="HD_SAS6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032396">
        <property type="entry name" value="SAS-6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44281">
        <property type="entry name" value="SPINDLE ASSEMBLY ABNORMAL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44281:SF2">
        <property type="entry name" value="SPINDLE ASSEMBLY ABNORMAL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16531">
        <property type="entry name" value="SAS-6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spindle assembly abnormal protein 6 N-terminal" evidence="2">
        <location>
            <begin position="63"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="202"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="292"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8045279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="595" mass="70299" checksum="29FCE23D3162D2DB" modified="2022-10-12" version="1">MQDYRGNFKRIEFGIQQTPAKSTTNMNDFQSSQKLKYDQMSNKPQSSNISFINAPITTVSKELFTLQIPVKVKQMTDDWNTQLILKMIMQQSQQQAHGQILRIELTDDNNLQFLQILDLTESEFLVLKNEQSLHVDFATFPMKLADLLQLCINSQRDEKVNFYVCLETKNGESTLAVIENNEFKKLTHLSLKLRSATDDVIKVFLSNKLAIEKQENEEQQKRNKKLTELLEEKHWELENLKSEIRKFTEDKDSAIQQLLLDEQKKFNDFREQTLSKETNFKRESENEKQFIIEKYDKIVLELQNKHSQLQQINSDLSEQRSLLIQQERELKNKLAILQQETLQYQKENQEFRQQNKELDTLKFTQERQLIELRIQKEGFEKLIRDKEEYLNNKQQLVDNEKKQNTILEEQVINQKKQIEKLESRINVMSEEVNKGNQIIEKLENELSKQKDKIKLKNTVVLQQEQTVQQLQDANDLNHKQINELRREKDSLEIKLKDQENMNLDLKNKLSESQKLLESNNQMIQYLNKCLNETKVQVPAQMPGISSLKSQTFTKYTSPIPLQEDRSFRNQSVTNQQNQNNISQISNATISNKFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JV57</accession>
    <name>A0A8S1JV57_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0060444</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JV57"/>
    <dbReference type="OMA" id="LYPHINQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="3">
        <location>
            <begin position="122"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="185"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="283"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="354"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8044360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="456" mass="52993" checksum="190A6F206BDC90C3" modified="2022-10-12" version="1">MASQSEEKDLIGSILSEDEIQLVPILYKRKKKLSLKSKEFNPEQHIKYDYIHNSNKSIISTGLGSQQKQRIFFHTQTSIEIQSQLSNALNQASDLEFFGSADNYQSFDSLEQLQLKQKVKKHLTVQPMEYQHTQFQFNSVQILDGKLQLNQKDLEQICGNQLINRKLQNVLDNNDRNQKRLIYCQVEKVCVKASKDMFGNYTVQKIFDVADYEQKYKLYSLLICHILELSKNQYAYSHELIQSLIKTLYPHINQLLNDLNGNYVLLSCFEILDKSKLLFIIPQIEECITILSKQTYGCRLIQKVLELYPLEITQRILDILISIAYQLCYQEFGNYIIQFLLRTGPQKEKSLICQIIKENFEQLSSNKFGSNTVEKYLDLIGPSQIIKILCTQSNNQFVFYNLSIHPFGNYVMKKVLISADPSVHYLISLLKQHPDLIQQIKNSEFGQRVGLILDTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVI6</accession>
    <name>A0A8S1JVI6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Membrane insertase YidC/Oxa/ALB C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0080054</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVI6"/>
    <dbReference type="OMA" id="THCARSQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF14">
        <property type="entry name" value="ALBINO3-LIKE PROTEIN 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="8">
        <location>
            <begin position="75"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8044490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="362" mass="41634" checksum="8D87D12E5AD5420C" modified="2022-10-12" version="1">MISRTLKLYKFSTKIPTGVFDFANVQQGITAPSSIMVEDLITRFSAYTPTNMYNSLDEYSRELLLYIANDLSWGMGWGILAISMGIKIAFTPLMFSAQLNACRMKLIEPESKNFQNMIQRAMRAQDFKASRAAQKQFKLFKRKHNINMLIPGLSILQMPFLFTWFLSLRYVCSLPDKYEGLKSQGFLWFQDLSEYDPYGILPIMSSFFTFWNISLNPNMQSQSTVPFAKYYRYVRFLPFFSIPVVIFFPAGVNLYWCSSAFCHLIITALARQEKIRKVFGIPKYLPGTILERQNNMNIQTIVKAVVQDRADLNSDVTTTTTTTTTTTPPQKQVQTDAQHIMHEAKSGEQKVKIFSSKPKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K074</accession>
    <name>A0A8S1K074_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0130032</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K074"/>
    <dbReference type="OMA" id="IYGCANN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF11">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M14" evidence="4">
        <location>
            <begin position="164"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8048679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="561" mass="66100" checksum="2C5FDA0159CBDB1C" modified="2022-10-12" version="1">MNKKLQLITTHNYSLINYLKVFQKKLQLLDTTIQPQSNQPNGQLTFITTQIQIYSLYDSSNLFYLTEDKDTINILLNSDSNTNGCTQWFHFQVQALKPTVTIKFKILNNRRSQQLLTNFKPYTSKSACFDLHYYKTNIHHPYYANDDSYIAKYQQLMPFYTLEFKFTFQDDEIITFAYAQPYPLSRALTLTHYKTIGYTQLNNPIIQIKRGKAKQYVVILARQHPSETSGSFVVEQLINIINSTKYRYIIYPMVNPDGVFLGNSRCNLHGVDLNRKWINPQLSSEPEVYYIKQSLEKYKQKIIMMVDLHGHSTKQHYFIYGCANNQNNNNEIKNLIAKFQNHSLFHLNYCSLETQANRKSTARQTFWYQYKVQFSITIEISLFGSQNGRFSNSDYKEIAYQIYNSIEKCNEPIGDQAALPMIKSLSDEQNESDSECEQDFVHDIVKKKDTYQETKKFLFKQKTIQAETSSIQQDQQLKQLLLPQIKTIKSMTKSEHKSSTKMEFENQMKVKQDSFQKNFNIINYFPQRNTQPKRMSSIKKELNLNKYPQIKIEQLKKYQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1V3</accession>
    <name>A0A8S1K1V3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0120472</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1V3"/>
    <dbReference type="OMA" id="ESCYDIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000004">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="10">
        <location>
            <begin position="49"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="11">
        <location>
            <begin position="248"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8048453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="449" mass="49576" checksum="21BF946C83EB86B8" modified="2022-10-12" version="1">MREVISIHVGQGGIQVGNACWELFCLEHGIQPDGQMPSDKTIGGGDDAFNTFFSETGAGKHVPRSVFLDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHTVGGGTGSGLGSLLLERLSVDYGKKSKLGFTIYPSPQISTAVVEPYNSILSTHSLLEHTDVCVMLDNEAIYDICRRNLDIERPTYTNLNRLIAQVISSLTASLRFDGALNVDITEFQTNLVPYPRIHFMLCSYAPIISAEKAYHEQLSVAEITNSAFEPANMMAKCDPRHGKYMACSMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVMRAVCMISNSTAIAEVFSRLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGIETAEGEGEEGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1Z0</accession>
    <name>A0A8S1K1Z0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0100236</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF379">
        <property type="entry name" value="CALPAIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Calpain catalytic" evidence="8">
        <location>
            <begin position="97"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8046206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="450" mass="52631" checksum="73336AE772383FAB" modified="2022-10-12" version="1">MADNIEKNESIQSYMNYSEQRLMNEDSFLKQKQTQEEDPQAFEQSETHSQNKQIDPMLQQNDEEQEQEIQPIELNPLQIFESKKMEIIQNCQKNKNLYTDPDFPINNSSLYKDPQKPPEFAKDIKSVKWVRPHEISKDAKFVIDFEGDYKQGAFGESWFVGAVVIVGQMKKYNEKQNKQTQQLSQQQISQLEKLILDYDQFDDCGFVAFQFFKNGEWKQVIVDTLLPFDQDSKQILFTQCANPSEFWLPLMEKAYCKLHGNYETICDGHIGEGLVDLTXCQLGHSLLVCLDSMLYNLLVLKHDSVQNEYDNTTGPTANDEQTLHDLKEELIAIWFLYILLPFRLGLWMIDLIIRYTGLRYFRKEYTTFAMFFDFLLGLLTIIIVGFSTRLDYSLRSDTAQTQVELIIRNLIIFLTFLAGMIQSKKAMDLTKRLCLLFNQRKVSCCNFISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2W1</accession>
    <name>A0A8S1K2W1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0130341</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2W1"/>
    <dbReference type="OMA" id="IVIDCQG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="125"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8049227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="429" mass="49006" checksum="29196E22DB06D9FB" modified="2022-10-12" version="1">MIIDNKDIQRVKQISKNNFMKRFVQNQRKYKEEGLFVGIRNVWLLKPLKFIVIDCQGLIIVQSRFYLINIKIMKSLILIALIAVAFTARVQERNMAKVQADLAKSTYGKALLHLVELHSMAGGPVQELIDAIEELINDLEEELETLEFNFQVRTNEHNALVVSLEQDIQDAVIDVNNTQDTLDNLLYPRRDQIQSKIDTVIGYQEENRKNYDEAVLVREQEHEAFEAQVAELNDATASVDDALALLSSLTNPSLLQIKRFQNTLKNIENKLRSRSRMAPMIKALISLASNQNFSNQDVLKSIVDALNDFRNAVVDQINDLTAQEAQDVADHEAYLEQLDSEFAEFGRQIDRATVDLTATNEKIDSMVEFRDQRAADQKQYTAELELENNTYAEETDIYTNTKNEFTRELGVSEQALALVKSVDFSNIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K378</accession>
    <name>A0A8S1K378_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dynein axonemal light chain 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0110369</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the dynein light chain LC1-type family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K378"/>
    <dbReference type="OMA" id="ESIWRAE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000049">
        <property type="entry name" value="Dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF73">
        <property type="entry name" value="DYNEIN AXONEMAL LIGHT CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8047320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="201" mass="22646" checksum="34552B396979D5FE" modified="2022-10-12" version="1">MAKTTCAKALQNWEQDHPGEQPSEAEDIRLIFQNPPLDKLDPPVLNTLAKVKRLSLSSNAIEKMVNLPGLRQIEILSLSRNNIKKIAGLEEIGQTLKELWLSYNYIERLDGLQPCVKLHTLYIGNNRIKVWDEVDKLKDLPEIANVLFVGNPIYENQKDDPKLLVLKRINTLKNVDGTVVDDSLMEKVKALGDGVPTTTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3T4</accession>
    <name>A0A8S1K3T4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0140280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3T4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="79" mass="9230" checksum="A542EA69A7F3EDB6" modified="2022-10-12" version="1">MQLVFQHISFEMQYIFPQDDIIQKLKIAQEQARVGNLIAVAECIKSIIHAPERTQEEILKYTNLYNQAVTYFNQISQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6Z3</accession>
    <name>A0A8S1K6Z3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0170094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit p55 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6Z3"/>
    <dbReference type="OMA" id="FICKHYR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000070">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054559">
        <property type="entry name" value="PSMD12-CSN4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040896">
        <property type="entry name" value="RPN5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22241">
        <property type="entry name" value="PSMD12-CSN4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18098">
        <property type="entry name" value="RPN5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="318"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8050511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="423" mass="50235" checksum="295A0F8075148490" modified="2022-10-12" version="1">MIIEENEFDKQIEDLLNKEKTARLANNLVETLKITKQIAKLCFDTKQYSKFNELIVQLSKKRGQPKKAQIELVQMAMVELKTLPINQKLEMIDAIMKVCEKKIYLEVEYARCVLMLTQYKEDDNQIADAAKILQEVQVETYGSMDKREKLEFILYQMKIMIKKLDYVRLYIISKKIEPKNIEDDNIADLKIIYYSFLVIYYRHENNYKETAHAYSKILESLHKNRQLEATKVDFNFRIDYNTILENYAMYTILSQYSEEKEKQLQSIVSTYKYGLEALPNLQQLIQAFLGTELISTSPQSHNIQAAEIFDENIENNKQRYIDFRRQLIHHNLRIFQIYYDSVYLNRITELIGISTQELEEEICLMMDQKLLKCKIDRILGIVDYQLKKNENDVLQEWGDNVNKVLNLIDLTSNLIKREEELFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K752</accession>
    <name>A0A8S1K752_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0170124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K752"/>
    <dbReference type="OMA" id="TRKFMEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="34634" checksum="1720FF8E6177D836" modified="2022-10-12" version="1">MKSPTYILKNAYQEHLVEQQYIKHVSKIQNTTANLQVSQSEQDQMNKIKYYRQQKERTRKFMEHQTKLFRQQSDNVLAKKMISADSKSRIIHEPKSVKSLIATAQKQQSYEIGLENIRLGKRILHLPSVIKKKEFDKSYAKHEQNMMYASRMRNQNKSKTSSNKFIKDIGPIFTEVPIQFKLVVFNKELQNKDQKTECLYCVEVYLKDQLLHRTDSVSFDQFQQLNFKVPMNEQQYEGYQNDPLILKIWKIKNNSELYMGQIFVGFNDQLSEKDLMKKVNDREIWNGFIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8R1</accession>
    <name>A0A8S1K8R1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0110137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HIFFNII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="283" mass="33211" checksum="D02BC42F58BA29A9" modified="2022-10-12" version="1">MYXKLIRAKKKIQITNIFRNLIQDSDQEKNESPLNQTVSGQISKQKSFFRMMSERNKNKRKIGIKSHSCDNINQLIILKNEEYGELPFDFLNEVVLQIHNDLLYDIDEHPINLQKHSIIIPESSEIINREPSFRISSLFGFDSPMNKLNQTNCASPNQIRRKISDFTSLVDKKQIINLKSTYISPLVLHESGFRSITEMSKSKKKNSYTKQEIDEIHQKTKPRIPHQFVNYNNNLKDLDNGLKKNEFQQKYSRASIVDLYSPHQIYLNIQDQCSCIECQKLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDD6</accession>
    <name>A0A8S1KDD6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0190109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDD6"/>
    <dbReference type="OMA" id="NSIMNQV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="301"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8052291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="650" mass="77001" checksum="25F1F5F2AEF9E3B8" modified="2022-10-12" version="1">MLFELNQNELRSYQEKYDKVMKAKVNLDFKKTKKIIIGQFSITEAQADLILKKVFSQQKSDELNREGFFTTLRYIQCLQNQVDIETCNINTIKLQFPLRVNSKQNLKKSFQPPNPEDLLDKSMIALKNLQNLVKEPPPLERQKSSTPSEIKCNPNQQIQKEKVENIENMEKISTQIINEIEQCNEIINQVQIQNNINEQTKQDQKIIENNIEDNTQFKEDKQPIINEIDDIYINENKTVQQSNITFTFGNNDAITQYLDNNQVIITESNVIPPKIQEKKPDFIEISEDEDDLLKRDESKDIFVWIPGYQSFKDGPFSQTYYTYTVCSEIRTDPHQGTYTKYQVHRRYTDFTMLQQYFTKSKLGDIIPSLPEREGVIASIKNLVVENAEFISQRQEQLQLFLQYLTKYKEDQILLSFLSNDKKFDLILKELRYCKGDNQDEDTFLPDPDEFTRKQKLKEMIVGKSIMDFVDYFKGEKEIEAKFQEEYRQVQQHYKNLENKKLELNKFKQCLNQFYEPIDKFSQISTLQLSSIVTEENIKYVLETQKLFQSNYSKHQNSIMNQVTQIEFQLNSLTYSFEQIQKQIYKWFAEKKRLREEKISNKLEIQKNDTEEGRLQFVISKTNETLIKLVSQITEDLKGLLKDFVEELTQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KFB4</accession>
    <name>A0A8S1KFB4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GPI transamidase subunit PIG-U</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0190191</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KFB4"/>
    <dbReference type="OMA" id="ALWHLWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009600">
        <property type="entry name" value="PIG-U"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN ANCHOR BIOSYNTHESIS CLASS U PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06728">
        <property type="entry name" value="PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035835381" description="GPI transamidase subunit PIG-U" evidence="11">
        <location>
            <begin position="21"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="273"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8052455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="48049" checksum="0B84D1002BA88BEB" modified="2022-10-12" version="1">MYRIILTLKCLLWFLGKWEVDLTWLPYFSNPYTQYNNIREAKYLLANNYNPYSTDGIFQKPLIVYGLVYIDNDHIFLIADILISFLIVSLFGAKQGYRKSILLFYHLNPITFANIILKNTNVFDHLFLLLTIYSALKIKLLSPILFGFLLYLNPQYFIVLAVISVIFRRETSTTQKFAFKLIAFLIVSIVTIVCLLHVSYIITGDWQFIEKTYIDYYFPKDHRPTIGFLWALFSGLFSKYKSLYHAFFMLLPSCYILPLYNLFVKYAKREKEYLGFMIGVSLFCSFLYFQYIAISDLVIIFILLFQRYDWLFNSLPTIVIIHAGCLILFLGGGLQLTWTHSFTGNPNFTFFQVFVSYVFYVILVSEGVREMLKFRTIQKKEENEKMKQNGEHQKKENELIDKKLKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KFD4</accession>
    <name>A0A8S1KFD4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190201</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KFD4"/>
    <dbReference type="OMA" id="CEYHLQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="67" mass="7816" checksum="41ACA3838F027997" modified="2022-10-12" version="1">MHLRSSSQGVSPQIEILQSERTLLNTPVTQSALFKTFTEMPKAYHNKVDLVRHSQKQYCEYHLQEPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KH87</accession>
    <name>A0A8S1KH87_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L13a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0220085</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KH87"/>
    <dbReference type="OMA" id="WRTAPGM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1180.10:FF:000009">
        <property type="entry name" value="60S ribosomal protein L13a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3250:FF:000001">
        <property type="entry name" value="60S ribosomal protein L13a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8054549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="23151" checksum="4CCA0231D445E100" modified="2022-10-12" version="1">MFEKEVVIDGKGHLQGRLASYIAKELQRGQRIVLVRAELIQQSGSLFRNRVIFEEYLNKRMAFNPRRGYKHYRTPSRCFWKVVRGMLQYKSKRGAAALERLKVFEGVPPPYDTRKRQVVPDALKLIRLKNHRPYCTLGDLCASVGWNQQSIVNRLEEKRKQRGATYYKRKIARENLRRKAIGAKELTGINAELEKLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIE1</accession>
    <name>A0A8S1KIE1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0220278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KIE1"/>
    <dbReference type="OMA" id="IKEIMHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37473">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37473:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="232"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8054929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="301" mass="36277" checksum="C146445B3FDBEAE3" modified="2022-10-12" version="1">MKTSPQSFQREMEETVWHTRKMRQETEKDIQLMKNRLKLLKRGDAQLSKRIDETKKKTQSMIDLKMNHHQQIEQKKLNQKNGEAQLKEKQQLNYDQKKQQEEQLEMIKKAMEQIKIEEYKKIKELSIRNAEMINKQKQDFVIKNKEKREHIKEIMHKSKSNISLYWNEKLSQIQKENEKAKLENKKACEHNKAYQEKMEMEESYMMQKLIRSQEVQKKLAIKLEKAKNLPHDEFNQMIKEEEDNSNNLKTNKSAEIPRWLPTPEKDAAIIQNLDTEPKQQSDQQKNEDDQQKNEDDQGQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KII5</accession>
    <name>A0A8S1KII5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0210072</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0210073</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KII5"/>
    <dbReference type="OMA" id="TAYFIMD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="159"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="363"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8053795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8053796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="382" mass="43702" checksum="A320168C0F915909" modified="2022-10-12" version="1">MDQAGYNHTQIQSLLHQSSNLMRPPRIGQQGSSIGQSEMFQYNPQQKQTLIQQYSSQNIGIAPFSARQIATTPIVSQNITQSQPLLITPSVRIASTQLNNAEVHHQERPLQVVTLDDMDSRWKTKCMQLEVYLFDLQQENLRLKGNAPQISYVQDDSKINELINANKELKIQEQGLRQSNKQLQDELDQWKLRYKQLQESNSKNQGADDEIRQLKKKISNLTDDLKNSQEQNEFKDQEIRKLKLLMNDKDNELESLDQRIRELEIQLESFSQSEQQIISLQGEVELWKKKFKQQNEINSDISEKLTMAETQLEALKKSKVTVTKESEIKRTGPSGTGITTQFETSSIKGATIAPGGYSTYGTNNSGRNSVVRNSGYIDRKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKT0</accession>
    <name>A0A8S1KKT0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0180182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKT0"/>
    <dbReference type="OMA" id="IFYFEPI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8051564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="329" mass="39272" checksum="336DB743D0EAFE06" modified="2022-10-12" version="1">MSNFLKYQKVVREEKIICLSKTIKKEKRKNQYTVEFKFGSDIDVSMLKQIIQVNQQIPAYIPLNITFRLESDILIPLQLDPNLQLGALAIDPISYNYRIIKYDKHQNNYIFYFEPIDRPFTPLEIVKNTLEKYHPSLGEQFELFHLNILKKDKFTIATEQEIQEYENSIKNSFAVYIQKKQKGRITTLIKSINTQYLQLMGINEEMLQDYINQTGLLPWCFCNENQQYMNLIKGMFQGAHQQINSSMPAINFNGQKFEVKITPRKFHIYNQDDDSYIQFMFFQQEYNSQIITQNQIAQNMIEYFNLKPKIIKQEIIEDDQIKRCKFRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KL88</accession>
    <name>A0A8S1KL88_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KL88"/>
    <dbReference type="OMA" id="VECQIFE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="1">
        <location>
            <begin position="233"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="417" mass="49059" checksum="7915ADFFCDF8E3EB" modified="2022-10-12" version="1">MYKIPCYKHEGSYVQFINTDKEKTQFICDQCQQDLFDLNPNFNFQNLINLNRGLKSPEFLFSKLEVSGDIRNFFGEYSKHDDISLNNQIKQFESLITEIQNGFIKMIDELQQNIKLFLDTKQIIRLDLEQIIKFNDFKQIMNKLVELGDSINPKAIEQAETDVHQYFQYISKDCKQLLIENIQENYQRNKQKIKNEETFMFSQFKRLESLFQSIIFKHNEFTKQLQPIPPKSYILSDQFYDRIISQIEIKSKKKILDSNLIFKGSRDGLNANSFWKCCNQKSNLLMIFKSYNKSIFGGYSPCKWVGEQYITHISDPTYSTFLFSQDKNEIYDQKDGFQNSAICCNIQGGPIFGAGHDLYIGNDFVDGYSKLGRTFKNPQLQQKNVWSFMETQEQSSYLFGQLTPNIVECQIFEITFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTL5</accession>
    <name>A0A8S1KTL5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0240114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTL5"/>
    <dbReference type="OMA" id="DMHEIAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="2">
        <location>
            <begin position="8"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8056046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="144" mass="17255" checksum="E1B4AA5E1A82A301" modified="2022-10-12" version="1">MTLQYENEQIERIYESEGKIQLIYDYTTMQGGKYQFCFVDKLGLYNEIYFEYLIGIEANDMHEIAQKSDLALIQETLNNIQAKLQFMKQQLIIFSQVKLENIFNLESLYYYIVKSDFIGFGIIFILIIMEVYILKRKILRKKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTY8</accession>
    <name>A0A8S1KTY8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0240180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTY8"/>
    <dbReference type="OMA" id="QCRTSIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8056176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="570" mass="66803" checksum="9E63DAD577D76203" modified="2022-10-12" version="1">MKRQINSALKSRQSPQSITRLKSAVQFKFQSETPQCRTSIAQMERLQTGTTMHTQYKTNFSTREDEVIKTQVSESNLSKVTNLRIRVDPKSETDVKMQNYFYNNQPIKVQSNMNIISKSSQKLVVTSDNLQTTEVKRKMKYDPAIRQQVFILKIKAIKNPLLQKFLRFLLSKEKQFIDLIKYKQNQQTIDLVQDFIEMAIGTQISLVVVEVLLFCSQALENCGLIELSIHLYNQTRLLCNHSKQQYDIQKMKAFIGLSNCAVTYESYEIAIKFLKKCIQYAWLNNNLEFENLVYQKMGICYFYMGNIEKASFYHERSITYDFEIEDSPLRKLSCDTLKIYLNKHFSRNYAETVNNALLSKMNFQIPIDIKQCQENLMNLTDLSQSPRLHLSTEGSRKMSISSENSELSCLKINGMRLLKDILSQQEFDFQVYTPKHSFRTESKCFEYLNNKKIHPKDIYHDAKYKTNPFVIDDLGIIKSKKTSQAENMSKYKLPLDQQIEIRLQQKFQINLQEKIQHYVGSSHQKAEQKNKILLTHKNQENSKRNKQMDEIKKFNAIAFLYQNLISKLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXY2</accession>
    <name>A0A8S1KXY2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0290329</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8060048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXY2"/>
    <dbReference type="OMA" id="MDDQIDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17996">
        <property type="entry name" value="DEXHc_SMARCA2_SMARCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000008">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003020">
        <property type="entry name" value="SNF2-related domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029295">
        <property type="entry name" value="SnAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14619">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01314">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="372"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="679"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="996"/>
            <end position="1024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8060048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1024" mass="122894" checksum="9998551777659266" modified="2022-10-12" version="1">MQQEADYNQDEKQVYQLRQQIICYKQVQRNHHIENEKLMFPYSKDQWETEREKIFQNSIQYFHDKIDKNEELKSIFRDRYPKLKLDQTCDDMSLFSERMQGYIEKRRQDIEQELKKTHQNDPKYVSLKIELLFINSKDFYLKVKESILNPLLQEENQAVLQSRMLERLLLDRNYFRRDKPQRRAESKLSDKFELSMIRHEQARRKKLKQKEFMSAIFAHQIEFMEFHRKKYKHARKRSVQFKVVLEQREQQRDKQMRMEHIRRGNLETYIQVLEKLDEAKKERVVSILRQTDQFLKDIGARVKIQKGEEITEEDEVIENMNSSNGLGYELSQANKVYYNITHKIKEVITQQPLLLEGGQLKQYQLQGLDWLVSLYNNNLNGILADEMGLGKTIQTISLLCYLIETKKNFGPYFIIVPLSTLSNWSNEFEKWAPSIKKITYKGSPQIRKEISKQMRTTKWNICLTTYEYVLKDKLTLSKYEWKYIIVDEGHRMKNSRSKFAMILGQQYQSERRLLLTGTPLQNNIAELWALLNFLLPKVFSSCEDFEKWFQTPLNIMGASEKDIQLDEEEQLLIINRLHQVLRPFLLRRVKKDVEKELPRKTEYVIKIKLSAWQKKIYDQINQRGVMTFDQQSGKSGSQALQNLMMQLRKICNHPYLFMLNLDMNKVTDEIWRSSGKFELLDRIIPKLLYFKHRLLIFSQMTQLMDIMEAFFEYRGWRYLRLDGSTKSEDRESRIQLFNQENSIYNIFLLSTRAGGLGLNLQSADTVVLFDSDWNPMMDLQAQDRAYRIGQKNEVRVLRLITATQIEGNILSKAEHKMGLDAVIIQAGLYNQRSTDQERRERLQDFFRQKNKVDLFEAEEIPDDTQINEWIARSEEEFEMFNELDRQRYEQEKLIYKNFNENKDDQYYNYRLIQEDEVPEWITSKQNEVQEVKEYGRGQRERKQVVYYLPEASPILEEQQDDMNELDFKMDDQIDIEQNQVYQDDLDLPKIQKKTKKLRQIENDDGNSNNIDENEPKLKSKRKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4L4</accession>
    <name>A0A8S1L4L4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0290121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4L4"/>
    <dbReference type="OMA" id="QILPEMW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="130"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="435"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="550" mass="65022" checksum="6896A412F4B58399" modified="2022-10-12" version="1">MHLIPKLTKLNKSQEDISAIQDYLSQLPQFQVPYEVLKDLSKTIGYIYFETGQIVQQNNKFALYVIKGLLQIEDNIQILPEMWYDGDLGFEVKQDTHCFALPIYLYKKFSSIHGVQYQQKKKSIIANLPIIEKIATKIQQQLIRLFQEVKFAHTDFILKMDEPGNALFILQQGVLSLSKNYQKALSHYEKQVLPRKFWFNEIVICELIDQGILGEEFTQQEKALYNVQVISKSASLLMISMQQLKNISPQVIQYVTKMFKDKTILRDEIFKQKCQQLEKNWQNTNKDQMLNQDIQKTISFKVFSKQPSSSQDQQSQTNDDQFGALFGNLSYKKMPTFIQQYFKQKHIKHNKIQDQYHLNYSNSTSINHIDSTFDPFILLQKTSQKMIVQQCSLKQQSGFRLTQTRQMDRKSELTKSQQIRPNTTQINVQQQSSIVQILQQQQQQQQQQQQQQQQQQQQYQQYQQQQQSINQIDIVSKQQLNIQKTILQNFHIQKPIIRQLKRIQRSSSVHKIDNIHKNKTPRDNMFYLNQQQNYLGQRNKVNQLLHNSID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L7C9</accession>
    <name>A0A8S1L7C9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0340265</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L7C9"/>
    <dbReference type="OMA" id="GNMPIPN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051019">
        <property type="entry name" value="VLCFA-Steroid_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899:SF13">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8063329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="293" mass="33285" checksum="A96198E4CC38E3BE" modified="2022-10-12" version="1">MEIIEWIGMIVVCYFGYLFIKEFKVMLFGNQNIQSKYGKGCWALVTGATDGIGKGFCQQLAQQGVNICIVARNKSKAENLIEELKKINDKPQYKIVIADFQNCLQESFFQKIYQEVKDLDIGLLINNVGVLTVGEFHKTSDIDQQNQIIINCIPVVFMTKYLLPILKKRQRSGVICLSSLTGRFAYPYYQVYCATKAFNDYFTRSLEIEVDNVDFLSLRPGYVQTAMVKNQSDLLTVSTKQCVTSALSQLGYKEATAGHLIHRIQTFIFCLAEGKLMDTISPIVAKQLYQQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L8B5</accession>
    <name>A0A8S1L8B5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0320186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L8B5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8061823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="284" mass="32989" checksum="50F78C6294986426" modified="2022-10-12" version="1">MHSFRLIYKNYSNSIFLYHYPQANYFPQQNYVSPMQMDAGFNSGLDALSRCPSVFIKQRPDYLETLGLCEKKNAYFVYQSDSMGNKPDFKQQAPIFKCKEESSCWQRNCLPGACRGFDLKVKQYNERQDTNTVFRMSREYKCTFLCFGRPVMEVQLENGGKIGSIYYPFMCCDKGVDILDENDQIVYQIRGSCCQWPFLVNLPCETCQRARFDIIDSQGQKISELWKESAGFCNALCDVDATNFRLLFPPQSSSRQKALLLSAALFIDFNYFEESPQDKQNNQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LD06</accession>
    <name>A0A8S1LD06_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0330210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LD06"/>
    <dbReference type="OMA" id="YLEFYVI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="35137" checksum="89AA088D42AD814F" modified="2022-10-12" version="1">MYSSFITKRPIGFSLLGTCMILDLFIAASQYSNYCEFFDKPIYKVSSDNGISIYQTNSEGCELSQNFELTYMGLNVSDQNAKAEVDNRITILNSINTELFACIALFVFYFHIFTSFLPLFLDFVLTISEYWMQRTRNTLMISLIRNLIVINWQLMQPLLLLTTVVNNITYKECFEVQDLRFLIPIYTTEWAILFIVVIVLETYTLFFFLVKWRNEFPCYLLIFQMFHFGIFHTVIIYNMVKGSKKLLQVLFTYKQWVTVFQIQKLYLEFYVIQERFRNHRIQQAKFAQLYNQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LD72</accession>
    <name>A0A8S1LD72_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0330270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LD72"/>
    <dbReference type="OMA" id="NMNTISR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210:SF1">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="1">
        <location>
            <begin position="343"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="455" mass="53225" checksum="758414AC028F7186" modified="2022-10-12" version="1">MSSTLNFSQILNNHLNNIMNPQSFIQPFNLQQLQRNAIATNGNENEMNIENFIPNNISSQIGNDQNIVALQTIKSEYNRDSPHLKINVYKQHQQTNQKTYILQDDKIIGGNQIHKNEIQIIRRKKTSRSSENNLDIGLTPIESNIDQVPCKISTEFGFKYSSRITPQILLFLSLKQFNSSFAALPSNVFRLIHQFIKEQPKFYIQDCGTSLQTLVRIQKDNPRIINENNKYLIGADFYFHVVQLSTYNLKFEDKRQQETDTDYFYQTLVREHIRDRARIHGLSKEEQDIFQKYLQNYTQMKKKGKKQYSLENCNRPFLKIQFDTPIVKQMAIFIGRPGGSNIFKIGRSQDCDIIVNMNTVSRKQTQIKFNVNQWEISDGEGIKQSANGTWQSLQQYENPLNPTQQKISQPLRIEDKMEIKISENIFRFDMVGFGIQKRIKLNNQLYKDLTNIDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LE30</accession>
    <name>A0A8S1LE30_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="350" mass="41489" checksum="B81C89BA67CF941E" modified="2022-10-12" version="1">MVKCLWCLKELGIFGMLFHKAVCLEYLYSKSLDKLKQAKQSQLITKEQYINEKRHLREYFMSRFKPKVYEAFIKRQKEQQYILINPNELSVGLESDIDVISKKVCFESQEISQKSQFLESFQNQIEPDYNYVVNNYSGNQEVYITNQLVIDDPNNNNDKQIDTNKHDEMNIHQLEIQNLRNIHKYNDQNDKSQDQSVQFQQNIIQHQNRDDIKEKPLNVVTQSNEEQKENILFKLLQKRAKPKLQKELNKSITQNQNVKQTHTRSKSRKIKEDLNIQETKQEELATEIKSIGITDVKTNKKKPPKFSQSLDKINSKIKQKXIQFSLFGMRWCIRNLKQFQSDGVHLRTCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LEV3</accession>
    <name>A0A8S1LEV3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TCTP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0240339</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0350298</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCTP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8064103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LEV3"/>
    <dbReference type="OMA" id="PYATVWA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034737">
        <property type="entry name" value="TCTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018105">
        <property type="entry name" value="Translational_control_tumour_p"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991">
        <property type="entry name" value="TRANSLATIONALLY CONTROLLED TUMOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991:SF0">
        <property type="entry name" value="TRANSLATIONALLY-CONTROLLED TUMOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00838">
        <property type="entry name" value="TCTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51797">
        <property type="entry name" value="TCTP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TCTP" evidence="2">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8056493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8064103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="173" mass="19408" checksum="B4783119D664F915" modified="2022-10-12" version="1">MKIYVDAFSGEEIVSDSFNMEEKFEGVIGEVQSQDIVKGALNVDVGAGGHFGGKNEDEEDAGVEDQAQKVNNIIDAFKYAETQFTKADYVTYFKTYAKKVKAYLEANKPNRVAPFQKGAGEFIKWVSANFNDLQFYCPESYDMENHIVLGYYKEGQAAPAFVYILDGLKEVKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIM5</accession>
    <name>A0A8S1LIM5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0380158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIM5"/>
    <dbReference type="OMA" id="INHDMIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000301">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039741">
        <property type="entry name" value="UDP-sugar_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952:SF2">
        <property type="entry name" value="LD24639P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952">
        <property type="entry name" value="UDP- GLUCOSE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="508"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="508"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8065882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="564" mass="65789" checksum="7DBDD55BCF8FFFA7" modified="2022-10-12" version="1">MKKIATNLSDYQFAGQGQLLKYIQTLREVEQNSIIHKLQKYDIKQLYHVYQQFKAKIVQMTPYETIKCLEFDGKDEEIGFTTIAEGKLAIVLSAQQNISLLDIQLPSHKCLFQLYCERIWSLQNIIKQRCGKCLPILIFIMTTNINHDMITSFFQDKNHFGLQEDQIFFIQQDKLPLLSMDGQILFSSEFQISDESIGNGYIYLNQSVLDTMKFLGITILHLCSIENVLCKFGDPIWIGAFIRNQLYFSAKCVQKRSVDENLGIIVNQFNQGLQYKEYDEISYNDLVKKDKNGSLVNSDGIIGQVLCSLDYAIELCCNYKKHILSNFHIRQKKCTYFDYITSRQIKPMQQPNALKFELTYYEAIPYCPIQQFGLFRVKREDEYAPILNSSNESKDTIHTARQAYMYRDQKWMSQLGFEVNSEFEISPKLTYFGEGLEEATKKQIKNKLQIPLILHSEKQIRTVRVNSVHNQQPQSSPNKYSSQTVNTQSFLQQKMSIHQSLNHLKQPITNSLVTPTNNQSFQLSGRKSIDQKPNRQTDYYHRPNLNQLNKPIYYVPNTYYLPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKG4</accession>
    <name>A0A8S1LKG4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Malectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0380116</name>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0380117</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the malectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LKG4"/>
    <dbReference type="OMA" id="NYICISH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039155">
        <property type="entry name" value="MLEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13460:SF0">
        <property type="entry name" value="MALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036273285" description="Malectin domain-containing protein" evidence="12">
        <location>
            <begin position="19"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Malectin" evidence="13">
        <location>
            <begin position="21"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="312"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8065823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8065824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="351" mass="40374" checksum="E1A17BF392C8D878" modified="2022-10-12" version="1">MIQLFIILALAFSKPLNPKKVLYAINCGAPSNYKSPSGIQYEKDIQTDPETVVADYSLNSEVSSQQIKYTREPEIYLTERHAYKTFSYQIPLQKEGTYTLILKFAEMYFDTKGKRVFHVKFGSQRVIEKLDIVAKVGKYASNDEYIEFEYIAGQVFHNNVLCVDAVQDGKLQVNLEKTKFDNPYIHGIVLYAGGIVETDYEEYQYMKENWDKVINEEKIKEEYERQKKSEAKVKRKERVKIRNDHLNVVENEFEFDDQSLSTSVPVAKKVGLKALITSPFGTAGLFISFFIGMSILNKIISTISNAKIKGIEKDITESKPKQEKQVKEKNDTSDKKNKTKETKDKSKAKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKZ0</accession>
    <name>A0A8S1LKZ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LKZ0"/>
    <dbReference type="OMA" id="RINLFNH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="132" mass="15744" checksum="0900EEE66FF997F5" modified="2022-10-12" version="1">MQKTPQRGRINLFNHLLQRRSLSSKQKTKRLNGIYIEGIRAPLDQRIQKAKQNKLQIKTEYLDYFVFETNETTIMPQTKMSSQRQPAIYNLQFTINKQLAKQKRIIRQPCDSFYTIQIYSKSLDKSEGRQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPN5</accession>
    <name>A0A8S1LPN5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPN5"/>
    <dbReference type="OMA" id="VKLDCTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="424" mass="48987" checksum="4909BD5CDF78865F" modified="2022-10-12" version="1">MNFTLEGKLKIDQFLQILKIFSYPIIICDDEKIQFSEIPENQKFNELNKSDKPASFAQINNDFFNEYQSQIRENHLKFAETSCLNIKMQQDVCLLNSQLNYVCCQIPIDIIYKALKPLAQSHIKILKLSFTYLLIEKGYDNKSIQLHFIVGAYSEKGFFTIMKPIAMLQSRDVELPNLKNKLITINYENCKTITQQMKTYPIGITCNVDEISFSPLSVDQEQVDIWSGALPYYNSSFVEEIYGNLSIQSIYTKTYNPILNYSSKVKLDCTIYISPSENDPKMAILYFVASESNVIICNRQQVITNDESKQQGKLFQDISEELLEEIEQINSQFEKKFSQIQITTNVKDNQNSCYDNQPLKMAYSAIDDNNNSKMYSTINQKEPLLFIPPKVTGSINDAILDYMTRNSCKQTTSTLPRYKSNYQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTM7</accession>
    <name>A0A8S1LTM7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0440143</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTM7"/>
    <dbReference type="OMA" id="RIHAQHM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8069672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="112" mass="13573" checksum="38CCE167D8944520" modified="2022-10-12" version="1">MLIPPKTKRHQTIDQQEYFFQQRKNFDPYIEMMKKQQQQEINIFRKAFANIASSRIHAQHLNEKREQIVLTVHKKKRIQAKSEFKLPTLGQGERTERVKIKFPPQRIKKIFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LX66</accession>
    <name>A0A8S1LX66_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LX66"/>
    <dbReference type="OMA" id="LSQPIMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="124" mass="14711" checksum="3C25F52EA9227274" modified="2022-10-12" version="1">MCDKRKRFNSALTFEEFVDYHNQLPETPQLKPIKQSTFYIPDLDLANDVCQNQLNIPLQTILEEELTSPKEIMKTKKSIRKRQRFFSENSQYSVINILERKLQKKKSFIPTPQNYDSLSQPIMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZQ3</accession>
    <name>A0A8S1LZQ3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0410305</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8068064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZQ3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="206" mass="24376" checksum="2CF805EBE5A61EB9" modified="2022-10-12" version="1">MQRSFPTQSQPVLSRKSKISELEENLFSKLDNLFIDQPKQEQVQTLQPKVDLLEQAKQRIQQKKEANPSFFEEGRKKLAAQRKKDTFLITAVNSMPQKQQKKQNQTKEYYKEETDCNNMLKDLIAQEERPYDDLAELRRMIAQTEQCMVEYVDDMHKLNKNVVKYNNEARKIGMGVDVQLFDFVDHKCNKYLTKSKTTTIIKDKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0A0</accession>
    <name>A0A8S1M0A0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0480154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0A0"/>
    <dbReference type="OMA" id="MNASCYN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035898490" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="738" mass="85971" checksum="97DF80985C1B3B41" modified="2022-10-12" version="1">MFIIIFSLLNISIQLNQQCNTILNKDCCIDQCEWENEQCQTKKAQNYCNSNNFNEEQCRQNPICAFYNGKCEPFFGCQTYQNIGSNCPRVSINCISITDEPVCIQKPINCIQISESKYCGQIDHQQGSKESCEWKDDKCQFTGCEKVNEFGQLSELKCQKFHNVCKFDGIECKIKLQYCSSYLYNCNQITAITGKCQLNSIKQSCMNASCYNYFEDSYEKCQKHFGQYGSQCIYNKDYFGGICLEIDYKTQVGKCMVEMQDKSCNQYLLISKCFGINSQKYDQKISFCQEGNIDLERRECNTSLNEQECNTIETIELKKCQWIKNTCQTINQCSDYELNFDSTEKTCDELNLGCKQIPCQNNIYGCCVQIQECNGYQTKQECTNNSDNFIIDLQYICIWENNSCRYGKCEDFNNDIYQCNNLEECRYIGKQCQNKKFISCEGLEIQDCIRLNCFYIMGKCYSANEIYKDIYSLSKTLEQDENTVCQYFSDKLMYSSDTRNCVLKSQCKNYSYNCQSMKQYDNVQCVNINSECRPMTSCTQLSYSEVEKCYKASSVCNTNGKTCEIAEKLCTSYKDQNSCNFQLDLSICQWDTNGCHLKKCEEIEDINQCDSKNLCMNPQVCRQNMCVQSNNYCITIKSRCSLYLSQISCYKSINSFCFWNGQYCMDLMNCQILSITQCQNEKYNHTCIFDYDFNECKNRKHSTSSSLNQTCLQNNYNYNYYCNTQENRDKIISSFKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0C7</accession>
    <name>A0A8S1M0C7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0470101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0C7"/>
    <dbReference type="OMA" id="TREMPHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="874"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="976"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="134"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="212"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="307"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="492"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="874"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="901"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="921"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="976"/>
            <end position="987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8071301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1019" mass="121354" checksum="6D250C7225F3C031" modified="2022-10-12" version="1">MSTELKRQQNFSRVQSTNINKAAEQWIVDTLAMDIRFKTLEEVGISRHQLMGRGLKKDTIDRLYKGLYVYSKGFNELLNQCIGDDILIVKGLIWKVFLILLERYNSSDYQMMMQQLMEQHFKEVKQVIKDNETIGFYNQELQNKLQEEQEKQEEQIQNHNKIEQNLKYQLDILNNRYLLADDAAERELEKRFRLENKTGQIRQQFSELVCKQQLLLDELQYLNKQLQQKQNQNEELLDERQNFLKRIEKLNEENNSLKIKIIDYTSTISLKNELMKNSDELITKLQNQIIKQQHQATTFVTNPFLQLKQKDDEYQQQEEEKLKQENQELRNRIMIYQQQIDIYIQQKSQDLLNQIKLLEDSLSKYKQSNQELQDKYFNKKQKLSNLKHKYNTLLVAQDITKGNLDINFKKIENLENANSFQIVNNSTLSQYVERLTNCIKKMEQNEKILNDRIHSQFQTIQTLSSQVEVTSRQNQLKIDNLNQLLLQKSIQNDELQLLYKDLKQSHQVMQEQYQKEVLQLQVQNEEQKQKIQNQEIAYYKLEQNEIQMKFELENQTKQYQEYIQKKEEQVKLINDQGSKIRKLEKDNARINQELNWVNNQNQGFSDKIKKQQLIIKDLQKDLDQVKIDYSQAQREFEDMKFDYDEQIIFYQQNIQSLVKQRKEFEEDLVIKQQTITKQAQIMADQDEKLKRQELNLMNKDQDIKLIEEKLMLESQKFNNLIIQKEEQSDQPDKSTIISQNIQEIIQKKLIQKIKQKRQQGNSNLITIIDSMENDNSIQDIIEHQLQNDSTLENLLNSIVEGPKIQTSSTQQLQNNGFQKQSNFQQKSQSILQTQNQTKLKQNIIGSKPIKPLPQFKQQQTIEILPQILTKSNDIISNQSSRNKTIKSSQKRPSKSMQKSETTQNYNTLQQNQPKQTYQSDSNLIQQQNQSRNQNLSFQVNVDTSFQETGQFPTQFMQQIYQNGEQINYKTISEKELQVDGENQEQRRQSQSRPRKSSVTKSKQFTQLYKVVADYYTQCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M1J8</accession>
    <name>A0A8S1M1J8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0480257</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8072145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="243" mass="28423" checksum="0C793CB084F52CA9" modified="2022-10-12" version="1">MLSCHHLTNTEIQMDKDDKPTLLKMFWNLLVCSFQTVFGYVCLDICVSVTLHYINKGMTQIDVAGYGFALNCVYIIVLPIGFGFNQSLNMHTAQAIGDGKHQLAKRFFNVNLYVMLMFLIPFSGILIAIKKPFSLIVEESQREQTADCAWQYLWFLIPAIILAIIFESIKIFMASYKITYPFAIIHFITLLLHWLFSWLFIXHSIIQFFQFYQRFLLIYLQIINLLISSKIFYLYSNSLYQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M439</accession>
    <name>A0A8S1M439_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0470076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M439"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035895410" evidence="1">
        <location>
            <begin position="22"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="270" mass="31301" checksum="FAD14A6FAE3DE1DC" modified="2022-10-12" version="1">MSVKILILLGLIVFITQQLVARCTCQQLFWKEECLIGGCFWKNNICIQVGCENRISESECNQITATNDWNPNCKWINEKCYDLDTYSCGYTCENSTMCEPDINGACEIKQILNRKHCSDFSTNNCNQQLSTYEFCEKHDHACTLVTYDTKCYQILTEKACLGLGCYWDNRNNLNKCNSKDCFYFDKQNCSWAINSQRQFTFCNWNETRAFCQESNNTTPFTNISRCSIDSNFIYHFDNSTGNQTCQACVDPQELYTHILKILSLTIMSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9A8</accession>
    <name>A0A8S1M9A8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0520249</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9A8"/>
    <dbReference type="OMA" id="VECLLML"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="507" mass="60147" checksum="796A9CF43034E346" modified="2022-10-12" version="1">MDNTKLKRDQFRLMLHSQQRLNQFQENRKRFLQIIMEEQQINELKLKVKVAIQQLNDNLTDFDFDCPNCELNTFTANIMQILPEMKKCIENLAKPDLEGLGNKFWNIYCFLLYNKDNPCFIEMLDLLTLGCQKSMVFCDSYCKSTLYNNLQTNVTQLSIQYQLEFLKQTVLIQSHQEHSLQYDKICEIIKQINAQIQISKNLEYQYKQIIQIIIDLFLTLRIEQVHKVALQYQINRYFLTLFTNLTSLDEILIGDELLNYIDYLNRNKTLFNISTIELINQLSFNSQLSSYPDEKSMAIAIIGVLMSGNLTQSKYYFDKFSTENLSLDQKLLQNLKLQPQVRRMTVFAFSNIFGEISYDKVDYFQNGTVIQFLQYARQEHEVEVIYEILQALRNLIYHAQENYLPILLQQDVVSIILECENYLLNEQQVECLLMLIYRFEQYDQQELQNLIQFPNFIQYLCFKGVANFVRKVFDSSKFGNSIQKQTNNDGQKDEYQNKLLKLCEIFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAQ7</accession>
    <name>A0A8S1MAQ7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAQ7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="491"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="521"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="555" mass="65735" checksum="EE2765D342F0B4E3" modified="2022-10-12" version="1">MNNNQIELENRSNSQFNSQVNSQQEIQLNQLDQSNETAINQLIQILNNQTNLPIDQFKQLVDLAVIDKQFLILTLNPKGIYFQNLRKLILLIVGFLAFQFYQLLYLFTQNQITQDHVDIKSKFILKTQQHIMNKSLSRTNLYLEEQINNLLIVIMIRIIYIIISYCSFCLIKPLSQHYSNKNLLLFFLLLIGCTYFIQGTQCNLYPNYETLHFEDIGKLSNQKIFWDNFIFFCCFFLQGMSSAIIFYLLLQQVQFILEENQRLIFYNDFSLVIIEFIVVSSVLHMIFKEQYNFNLIIMWIPFSLITLYAFFTIQDSPLCILDRISYYQQISKQNQQNLKKQQVAINKIDDLITEFQSNLNYIYSTNFQNKVYFSEEQVSLLLNKFQFKISNKQEPSYIVNFIMFTLSLLYFGHLFKLYVFIYQLQWSVQTLALILSIFELLGYFIGNYCISQLYNQHLLRKLLMTFGLINYLSSLPSFQSIHSSFQSQVEFFSILIIRLLFSLTIKAFLIQFSQYKLNFGFLMLGLLIANINLLQIQLFQLIIATILIFAYRLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCV6</accession>
    <name>A0A8S1MCV6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPIase cyclophilin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0590148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCV6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF6">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8078067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="66" mass="7308" checksum="D7F899D98B8D6144" modified="2022-10-12" version="1">MIQTGCLKGNGTGGEFIWSGEFQDEFHPEQINDKPFTVSMANSGPNNNASQFFITVCPTPWLNDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDX5</accession>
    <name>A0A8S1MDX5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0600122</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HECTQEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038800">
        <property type="entry name" value="CCDC17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33820">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33820:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="780"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="98"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="791"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8078557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1530" mass="179260" checksum="A0F724A3E3DC0F57" modified="2022-10-12" version="1">MNQSTLQQSTALNSSIAQSHISKIMNKTYLLSEHDSQMREVLQISYKDQHQSFKNEISMIQGAKDSITTGVYLSNIQKERADQFDVVLAQRKFLWKQEEQLYQQIDDLKKTNKRFQDSVSHYGVRNHNLELEVKQKYDSIQTKLNSVREEKKELLKNFEQIQQKQTTSRSKFSNVGQQILENPQYRSMMDFINQQEKKVEAMKIEFNDPASNQIIPFDKKLQQMAENTFEKQARFLYQGTKFEGNDQQKLAALSTLRNKVEQYIEDRGLQGVILDMPFKLNMTKEQESAHKVEYNQTLTEIDKIRNDYIQSGGTDPNFLISVNNLERYYKKNHPIGKEEMPDKLTMNLEERGPITQNPIFQYLPQTFQKEILELENQVQIQREADRRNPNLNKELLRGESIDVDDSSKADEMMLENLRQQEISILLTDNMTEENKKKYDEIQRLKQIYEQRYLEKKVQEQLELKQVEEDVLNFKGRPQDKLNYLQYLQGQIGTMPLHYDPDGGFIVRLDFVNKLPIIYENVKISYGLFIRNLDDPHTTKTTQQHECTQENIFQKKCIIQERFIERNHEIYKDTYLYLILWCYNQEYGNGAVPIQVGWTLHKVFDEENLISGCFLLPFYNTSFTYDLYKQVEIQEIKLGLRISMPGDAVLDLDTKIINIVDYKLQPLHQKQQDVIDLEKQFKFKEYRVFPIPKEFLNMQCNNPSLWKSELPPNVYKLMDFGFLNEQQAALKQQQLEYNERQQNRLNRPNRRLFGKQGTAIRGLPDKKRTVLTFKQRLALKKGTVTPNTNKSKSRDKEPTQRPDSKADTSRQKITEGRSAQRVQEKEKSTIMSTKPRKLIFKLRSLSQLHAGSITSFYLKLALFQNIKLIEDDSKNMCVFNKELEPDEKGDNYFNYTETFTFDLNLTNYFQEQNQEDILNTYLFMAVFKDKLDLFGWHALQVFDFTDNQYKINSGIYSENLYGPPGQAPPFNLNRTKRTNNQINFIIMQDDDRVLQLPTYDNYKLDQKLLTEEDREQKKKLYSVANPKWNTQIRLDITLLKNFSQKEDFIMKTFVIEGENIIIDVLDRQCIRHDNIELFDGQGKGEMIVNHTIILRIGTRYLIDTFTQTYKKLAYLFNFIQKNEIIGSCQMNLFTSSGTLNIGQQSIFVHDLDASGKIGKKTNKQMYFFIQEENLNLIDIEETPKYAQHIKDKQQNLQFDQNKGLVIQIDYLNDYVNQKPVDFTIQIYNGNEPAIDEDGVICAYTSSKKYQPSSNQSVTINGFAYFKLPLTQLLHKKVELDKFYVFISFQDLGWISFQLFLNGQLNSSEFVGKLYQGEVPAPPVDYTSIKKINTKISYSVRFDYKPIEEHNDDEIQESHPQINKSGASKIQQSIPSEQVAKIASRRTMNSTENTDKLIIVLINVNGFLNQAVLIIKGLLMMDSKPILDKNNQTCSFKTDKVNNVQKVAFFNKMKFAFLLDTTILNMESYFFISVLDENETVIAWFGRRLVSSVGKVIRGQQFEYLFAPPLMRPPLDKSKITTLQQSIQFEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEC0</accession>
    <name>A0A8S1MEC0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0610052</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8078960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEC0"/>
    <dbReference type="OMA" id="CNIMFIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01861">
        <property type="entry name" value="Rab6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002489">
        <property type="entry name" value="Small GTP binding protein rab6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="191"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="191"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8078960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="216" mass="24245" checksum="A23136B4B0103D43" modified="2022-10-12" version="1">MTDTKGSMVAPLSKYKLVFLGDQSVGKTSIINRFMFDTFDGKDHPTVGIDFISKTLYYEDRTIRLQLWDTAGQERFRSLIPSYIRDSAVAVVCYDVEVKQSFDSVVKWIEDVRLERGNDVIIFLVANKIDLENRVVSTEQGATLAKEQDAHFIEVSAKSGSNVELLFKQIAATLPGTETSQMVNSVVNNPIQPKNNINLDNPQNQDQEKKDGKSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MGE7</accession>
    <name>A0A8S1MGE7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0560224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MGE7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="499" mass="59604" checksum="A02ADA5812CAE0B9" modified="2022-10-12" version="1">MPFIQLKSTMTNLDFLLIKPNTGEKYYYPVCNFIKIGFQQIVQKNSSISKMLQMDSILRIILINVLIAQKFMKRFQILYHMISIQELRMTSILLQIKFLFYMEFYKVTLKEIKEDLGTHQHLIKFNNKQNCYQQRIMKINLKKHNNFLIQPSLIKNLNQIINFNSGLYVDLIMQIQTFQQDIRIANMLKLMNYVTRLIGYQIENYRDLQNKQLQEKESNSQSFQQILIYIQNVLKQVVHCLVVDFELLKAITRSYPGNQLFVVSKLNNDEITLSDYISNNVSNKDQFISTYWNNNKLDKLFQLSFQEFQRIVLEKMVTVINLELHRNVKEKINQCKHSNCLMKLKDQSGQMIELPARCVNCPIPKENESYYSETKISLLNWDIRTFVTFIVHKQNKKETSENYNALYVKLNLEGIQFKFNLTINQFFMMLSYIRKCRLTQKKLVIGQIICSHIREKNTYQKISRIYGNIPVKIFKVIKKIVKFKRYIMNQFIVQILKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJB2</accession>
    <name>A0A8S1MJB2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Transmembrane protein 230</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPRIM_AZ9-3.1.T0600116</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Involved in trafficking and recycling of synaptic vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
            <location evidence="5">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Synaptic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TMEM134/TMEM230 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJB2"/>
    <dbReference type="OMA" id="YKIIQFA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044234">
        <property type="entry name" value="TMEM230"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008590">
        <property type="entry name" value="TMEM_230/134"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15664">
        <property type="entry name" value="C20ORF30 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15664:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05915">
        <property type="entry name" value="TMEM_230_134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="9 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00007743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8078551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="122" mass="14131" checksum="CA4DE550568964BB" modified="2022-10-12" version="1">MNLKPHKLGQIDEFELQNTSKEDSHITISSLKEQQLFHLNAIPKKIIAITIMLTILGITFLIIGLLLLIKQPSNYDQYLPLLFIGILMTIPGIYYSYKIIQFANSRSRQEQEQIIQELPIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJD9</accession>
    <name>A0A8S1MJD9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0610078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJD9"/>
    <dbReference type="OMA" id="RSKNCIL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8079012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="37886" checksum="0E5CF2CAC461B367" modified="2022-10-12" version="1">MQQQLQRHGSMEPLNDQNAQVRGNALDQNLLRSKNCILWLLCFCFLFCTIYLQALCMIPGLQVCSMNGIEAPVPFQQSIIAYYFFTLLFLYMIYYALENVKHSIVVYIRWPLLLYALAAIIIGVISIISSYSLTTDDLQEGWNKMTQWRKQQYYESSSTNLLDENKRNLILISIYHFIYGFIFIVLFGLLFNYQTIMPQNWRPPFSSRLVLRQTLIYREGMIQQPKQDDEENQENKQDKPFQDYNQNEIRQQQQQQQQSIDQQPSTIFQKKNTGRRVFGKQVQNSQVQQQSQQQSQIQQSQIPPQPNPHQDQPEEGNQQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MN15</accession>
    <name>A0A8S1MN15_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="18">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PPRIM_AZ9-3.1.T0600153</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Na(+)(in) + ATP + H2O = Na(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:14634"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="18"/>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MN15"/>
    <dbReference type="OMA" id="MWIAFND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008554">
        <property type="term" value="F:P-type sodium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000061">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000045">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000191">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 18" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0915">Sodium</keyword>
    <keyword evidence="15" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="340"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="791"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="824"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="864"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="907"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="947"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="977"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="43"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1034"/>
            <end position="1058"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAD8078593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1058" mass="118590" checksum="D7FFF7AE22978063" modified="2022-10-12" version="1">MQQQYSKQENSRDFEEFHNIEEFKELFLLNSINDGSSFQKVLKLGGDQGLAKKLKSHLLKGIDSEDQVVENREKFGNNDPIEKEPSSLCELILECFGDTMLQILLVAALISTIIGIINEGIKTGWTEGATIFLAVFLIVSITAGNNYLKERQFQQLRRKLDDGMVQVVRGGIVEISIKEIVVGDILQFGIGDIFQVDGLMIQGSQIKVDESPMTGESDEIKKLPFNEMSQQQQSSKDHYHFSPFLISGTRCLDGNGYMLVLQVGQNTVQGQLKQLLNQDNPPTPLQQKLEGVAENIGKLGTLVAILTFIALMGHLIYDVFVDHKHEFFTLLSLQLIIEAFMIGVTIIVVAVPEGLPLAVTIALAYSVGKMKDEQNLVKNLASCEIMGGANNICSDKTGTLTQNIMQVTALWIENHTYNNQEINITSKISKQSIEIMSESICYNSIANPTKDRDTNRWIQIGNKTECALIELADNFGYKYSNFRQNDKILRQIPFSSKRKKMVTAILNPKNQTIRIFSKGASEIILAQCFRYISTNGTELVLDKVKKDEILHNVIENFASRSLRTIAIAYKDLEPQSQAIKGFVNAKANVHQINEDEIDKDLTLIAIAGIKDPIRPDVADSIRQCTKSGVTVRMVTGDNIITAQSIALECGILEKNRAQQEFEVIEGKKFRDLVGGLISAKNEDGNEIKVVKNMQIFSKISREMKVMARASPEDKYLLVTGLIQEGNVVAVTGDGTNDAPALKKADVGFAMGITGSDVAKDAADIILIDDNFSSIITAMKWGRNIYDCIRKFIQFQLTVNLVALFMSFTGAVILKQSPLNAIEMLWVNLIMDTFASLALATEPPSIKVLDRQPYKRTDQIVSPKMYRTIVGASLYQIIVLTFILFLLPKFIDCSIPEELIGQKYPMNVVQMSIFFQAFVLMQVFNSISCRQLDYHTKNPFANFCNNPLFWIVQTITVIVQILLIQYGGKYVKVSHLTLFQHLLCVGFGIGGMIFSVLFKFIPEELCQKIHLFREEEIKTEKMDDTLTSKLRRKSTMRLHTSQRSKHDIGSLNKMSSDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQN7</accession>
    <name>A0A8S1MQN7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0670276</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQN7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="198" mass="23790" checksum="2404FD1A43B8FAD8" modified="2022-10-12" version="1">MKALANNFQMDLNKNRASFEKRGQLILNFNLNNQNCYKNPYQDFRFQPSNPQNSAIQYYNKLLPYKSFYLNCLIKKSLCCSYHRFTQIYTIYTQKLQNLSCLFSVHSKDHVLMNKLQIAYKKFKLLLVPRNKFHFFPQGQCNHNVTFQNYQQIAHQSSSLKINHPAFIIPKMRVTIRSLRLGSILFKYNWKQKEDYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSS2</accession>
    <name>A0A8S1MSS2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0700055</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSS2"/>
    <dbReference type="OMA" id="EECQYCH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045234">
        <property type="entry name" value="Unkempt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057444">
        <property type="entry name" value="Znf-CCCH_AtC3H23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493:SF50">
        <property type="entry name" value="RING FINGER PROTEIN UNKEMPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493">
        <property type="entry name" value="UNKEMPT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25512">
        <property type="entry name" value="zf-CCCH_AtC3H23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="316"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="325"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8083428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="483" mass="57232" checksum="4BC48BC85E007049" modified="2022-10-12" version="1">MLGSRRVRSHESSLTNLDVYNPNSPVRIQQHINHHQFKTQKCQIQHQINQKKFCPFFHDETDRRRDLKYYSYKCQLCPQADNCPQGDECQFAHNKVEQVYHPNRYKTKYCTHIKDCDYGFYCSFAHNDQELIIPVKLDGMVQDKNFWMYQYKTVWCPLTINHDRASCVYAHNAQDFRRDPKKLQPKECPHWNKTNQILNYDKGGCPDQEECQYCHGWKEFEYHPLIYKTKPCTQSNCNKKLAECAFYHSDQEKRIRKQLADNQWVMEEPNIHVEAKRQPYKPTSNYLGPIIPNYIPQDYMSKEKMEIGQPFCQQSITNTKTSDSNSRRGSDFSDGSKMHKKKHNIQQQFQPQKKHRTAPTTPDQKQLHIMGNNYTIKAQQNVQINSHLSYSKKLYEEILKLNEVNFIYLYQFQGECIYKILQGFKISEQMLMQMGDQQIKQLNLSEQQIIQLTSALAAIKIEKKYDEHCGDELLSLISNQGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTG1</accession>
    <name>A0A8S1MTG1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0630204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTG1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="511" mass="60031" checksum="13EB05B559C2627F" modified="2022-10-12" version="1">MTEAQQLEEFTIYGIQSEFSARGQIKYEDLRMLISDYMQMQPIFFLIVQILEQEHQIQIVEKIKIDIQTIFSTFRSLSFLQEVPRFLNGRLTHLNVRNIKAAIQLYHDGLLTMKDIKDACLIFHLREQELMSEGVPCNATEINNLLWAIGKAISSSVLEDWIKRCTDFLQIKGFIQEFEFLYLMANTVDRWAYYEKIPKTKMDLTRDPKMDNLYVVDQTGYKTAKNPDAKIQHYMNYQYNMDKAVFKGRSLKDRNEYYIQKRTQKHQQFLKNKDAGIGEFKKLLRDPELYVEIKSKLKNAQQILNHSKLNGKMLRVQLLEEFAGSKEFLQSQSGQHNKQLQQEESRKSIPTIKQLKEHPQFQKQTQKIQISEAIEDIRLDVQQHKKMMQDPAFMTEYLQRTSSAVSTKPSMRKNTSGAVQSQTSLRPSTALVMPTYTKVSRPITAISNQEHQKPRLSQTKIPQILDEEIEIFQNEQQENKNQNTVFYGSSNSTKRLYNIDLPWHKMQLQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTW6</accession>
    <name>A0A8S1MTW6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0600015</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTW6"/>
    <dbReference type="OMA" id="FNMAPEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000131">
        <property type="entry name" value="Dual specificity protein kinase TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="19"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8078374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="632" mass="75101" checksum="7E9FEED879BFB4F7" modified="2022-10-12" version="1">MQSSINTIEAIKKQFGNIYKNLEKCGQGSYSEVFKCQNQNGEYVAIKVIDKSSIDSDQVPYFNNEKELLSINNNQNVIKLYEYQESDQTLFFVLEFCQMDVAQMMTKYYNNRIPEDIVIIILKNIMNGLQSLHKNNIIHRDLKLENIGVVIKEENLIKLASKQENKYDLIFKQASYKLLDLGLAKQVTNQTKTFAGTQYYMAPEVIDGRFYSFQVDMYSLGVCLYQMITGKHPYKGNNYYTQYKKIKQQEANFQVIENQLLRNIIQQMLQFDVEKRLKFQELYQSQLIKNLQETQPSILEISLTMNTQFYIINESNIIQKEDIQQENEQKIQNQQIHQQIQNNTQFFENFKNSQLYSQTQESSIIQTQEFSVIEYPQQQKKSQIGKSKSPLEQNQEIIQVKEITRWKKQKNICMLIQRLCKKLQIFIQQIPSDINYSSFEGEFTYYLKYYCKFSEKLLKELQVEVNSKNHYQKIQEYQIFKDSLKNIQLINLQDMQSNLFSNLIYTSRTYFNQVNSIFEIKDDNTLENQKNFLQKNNKIIFKQIRKISQNLYYKEDQAMHQFTQVQCLIYLILVDLAILYAEYLNGNTILDDTQIIELKPEDFQNNPDHICSFLFDIMEQNKLSFTDIQDIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXI5</accession>
    <name>A0A8S1MXI5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0730129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8085068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXI5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8085068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="104" mass="12375" checksum="DC80EFE42676DE14" modified="2022-10-12" version="1">MNRFKSKYKLPPKLNTQPIDPRVSYYEVKLQELSSQPSQKYKVQQSTSLTHEGFVRQRKTISDYNKLQTPLPKIQTVRKYNEEYRNIFSQRNLTRLVGGLQQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MYQ0</accession>
    <name>A0A8S1MYQ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0720045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MYQ0"/>
    <dbReference type="OMA" id="NYEIVKV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="1">
        <location>
            <begin position="211"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="300" mass="33278" checksum="01E459916D402053" modified="2022-10-12" version="1">MFSRILKQNYMTKIALFSTASFIYWGSRKNQSEEIFQILNKQTQNSIVKFFLNGRIIGQGAVLKNGFVVTSSEVFENQQPQISAQINGQLQNYEIVKVEEGIAILKIQQQKSQFNLSKYEIGQNAYVLGTDEQGLKEFQQCPITDSNFTLDIRLVGEEALNQTFPFILMNGCNSSPGSPVLNKNGDLLGIISGKFRNKTQVVPSQYFQGLENGKSVVKPYLGLTLKTSDQGGAFIIKINSDSPAEKAGLKLGEIIKSIDGVSIQHGKDVTKLLGVTENVDSHVMVVLRNGKERTVYVNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZH6</accession>
    <name>A0A8S1MZH6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0680190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZH6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="90" mass="10505" checksum="4082A1AF48D671C7" modified="2022-10-12" version="1">MRYYRDEQKRFINYFINQSIIFFQENSTLNNIALLKQVFYCDTNTTCAAIAQDIAYIINSTVQTYFGNTTSQSCQDKIRLNVPTTLVIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0T0</accession>
    <name>A0A8S1N0T0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0760056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0T0"/>
    <dbReference type="OMA" id="IQKPKYD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8086200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="131" mass="15574" checksum="31CCF64326B902D3" modified="2022-10-12" version="1">MNQRKRVKTLLDYNKIERDLPNSELTEQEKSIVGLAIRERKFNLRTRIKSVNSKAEFCKNYREYEDKMKLSIAQIVDFEYKQSRQSESTVKHKGQFTTIFSIPKPRYDPSIVYGSQNNKRVVNIKEILKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1T5</accession>
    <name>A0A8S1N1T5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aurora kinase</fullName>
            <ecNumber evidence="11">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0740214</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="11"/>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1T5"/>
    <dbReference type="OMA" id="EMRSTFC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="87"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="366"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="626"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="728"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="397"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="636"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="214"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="9">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8085592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="856" mass="99442" checksum="035D0E0D96C1C1C1" modified="2022-10-12" version="1">MKKSSLCDNSKCNKTTIFQKQMAPVKCSKCNSGTYCCQACLKEDEDHICNSDHLDLDNTTQINSSVNETLLQIQQKKRTLDDYEFINGDKGMLGRGAFGEVRLAIDIDLQQEMAIKILNKKKMLRNCNLQQLKNEIKLQRSLNHPNIIQLYHAFEDRENIYFALEYASNGSLYKYLRKLKVMPEPEAFVYFFQTCLAVDYLHKKNVIHRDLKPENILLDDQGNVKLCDFGWSAESVEMRSTFCGTFDYMAPEMLHNKPHDYRVDIWALGILLYELLHGNAPFTKAHFAKENIQNLNIKFSQSVSSQAKDLITQILQHDPKKRMSMNQIFAHQWLQSNAEEFGMDISEYIYVPKSQRINDSSINQTFEQSKQLNESKIKQNNNTNTSQIKKPDFLNTSQQQQSYQKQEVQKIQQNSQENKKITNNNSFHQTNNQSLHQTNNQSHISKISENLQDSVRSSSDCSDIQSRASNIRQSFAKDLSMKGLSQNNNSFHTQENKHLFTNDANILNQEQIERLLRQQLKSAETKSDDIKLSLNSFQNIQSNQLQNKSQSSILVDDNKNFSQQLYSNQSNTVKQRHVQFLSPKESMNETSQKIQQQVEESPYFNPAQIRESIADDEVKEKFSFGSLSNSKHDQNQQEIQQPEEQQGFNKKDKILKETRLSKLSVIESDESECKSDGEDQRNYQLQQITQAVRSYSANTIQPKDTKNSRNPQEIELQSQFLYQNNVLQDTKSQTSKTSKKSKLSKQSKQSTNTKVNSEQSRAQQQLIMQKKKYSQNMVDYRNFNMKEISKEDRNRYQDYKDSTKQLEIRSRELQAYLYSQDNQFSSRRSIKQNNASKEELGFFGKIQLAFGCLGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2E5</accession>
    <name>A0A8S1N2E5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phorbol-ester/DAG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0690203</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2E5"/>
    <dbReference type="OMA" id="DICEHCW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="2">
        <location>
            <begin position="46"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="231" mass="27506" checksum="44A38D5946DC1042" modified="2022-10-12" version="1">MSQIRHCRICNNAHKFKIKGLLSYPKHFPDKSIHKYYQRLMKKFEEHNIQQVPFSDDHICYNIENDICEHCWLFMHQGFQCSKCQIYIHNNDKESKICTLCNAQYCIYCENPLELFSQNKLCQTCEQNKNESFGTPSFLISFFISLLIPCFACSFFLKKMIKTIERFQYSTYQYTFAIITFLIIFPFIYIISIIGFLALLIFKLSICILDFLQSSKTVRMISKYLCVYKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4J1</accession>
    <name>A0A8S1N4J1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PPRIM_AZ9-3.1.T0790067</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4J1"/>
    <dbReference type="OMA" id="DPGKKTD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000632">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000522">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="67"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="365"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="407"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="443"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="480"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAD8087698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="508" mass="59312" checksum="B6F1A91FF65E2076" modified="2022-10-12" version="1">MGNTCCSNQPIDPEKQIDERPEDPGKKTDQEPKNVEEPNNQIIQYPTLSVQPSLFIQMKRETIYSTYQVGKLLGEGAYGQVSIVTHRVTGMQRAMKAIRKDCLFEEEQAKLFSEMTILKNLNHPHIVNLFELYEDEKFYYLITEYLRGGELFDRIQKAKSFSEADAVRYMKQVISAVAYCHSNNIVHRDLKPENIIFASEDQYSTLKVIDFGTSRKFDKNQNMSKRLGTPYYIAPEVLQKKYNEKCDVWSCGVILYILLAGYPPFYGRNETEIFDKILKGKIPFHTTEWNKISKEAKNLITNMLCQDVEKRYSAQQVLDDPWMQQGQEQNLFDDNFLKNLTEFSAKSKLKQALLTFMASQMIQPKEVEQIQEFFKQLDKNNDGKLSKEELVAAFQQKVQSKDRLIENMETKINKIVTEIDVNQSGYIDYTEFIMACLKYEKLLTIDKIKQTFRIFDLDGDKYISKEELSQIMEGVDDDIWKQFLTECDQDNDGKISEEEFINLLQDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5B8</accession>
    <name>A0A8S1N5B8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0790027</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5B8"/>
    <dbReference type="OMA" id="AWIVIDD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF14">
        <property type="entry name" value="CYTOCHROME B5 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="5">
        <location>
            <begin position="5"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="5">
        <location>
            <begin position="104"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8087620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="214" mass="25075" checksum="A7B47435863DC390" modified="2022-10-12" version="1">MSSEYPIISWKELIKHFKRSSLWVVIEGMVYDVTTYLDKHPGGEEILRKCGAKDATEQFLEYNHSNYARSILASRIVGQLTDEPPPHNYAQLLKQRKQRIKNPYKQITWEELEFHNTSDDAWIVIDDDVYDVTDFLSEHPGGMKLLLDYAGDDASKFFHRINHSQYAHQIMNELQVGMIVGIKPKKKEKQAPTNYVLIMFIIIVLFIFIYLFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7P7</accession>
    <name>A0A8S1N7P7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0780064</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7P7"/>
    <dbReference type="OMA" id="PTRLKCG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051438">
        <property type="entry name" value="RNF_E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016:SF1">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016">
        <property type="entry name" value="ZINC FINGER, RING/FYVE/PHD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="22"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8087169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="220" mass="25616" checksum="3C76801B38883C71" modified="2022-10-12" version="1">MQRKKLERNFVNEQSISKYLKCIICAGVYDEPTRLKCGHTFCKTCIIEWLNNHPNCPECRANTQQKHFQTDRIAAGIISELDVYCLNRKYGCKWKDVLDQLENHQKKCTAKSVATQLNLKIQSYQDNDEDNELAQQNDPGNLVTRIYANLKDKVEVIDALKSSNENTKDNNLEDDDPLAFLEELQKFSDKSPLEEKREITFSDVDIPKQCRERIIQQQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9A7</accession>
    <name>A0A8S1N9A7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FHA domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0770111</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9A7"/>
    <dbReference type="OMA" id="QNNFYCV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051728">
        <property type="entry name" value="RING-FYVE_E3_ubiquitin-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879">
        <property type="entry name" value="CASPASE REGULATOR, RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAM" evidence="4">
        <location>
            <begin position="17"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="161"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="265"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="358"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8086816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="46771" checksum="4E802C80B61BB3E2" modified="2022-10-12" version="1">MDYDSLYLLLPKEPKKWQVSDVIIWLQFIGLGQMEDKFVNCSIDGSILEEITENDLEEELGINQKILKKKLMNWITTGLKEYSIYLSQIKINTIKKQPNEEFTLQKNVNACQDKQCQSIINQYDELYSSQNKVTNLIENQLILQPLESQQNNFYCVKQVGAKIGRHSSNQILILEENISRFHAEITYQDSKFYIRDIGSTTGTYIKIQKRMNLFLDMLIELGSNLFQVTQLEEVSNGIQLGLLVLEGPNSEEQISFHLNQDKTSVNFGRKANADIAFPEDHHLSNLHAKFYLVDKNVTIEDHSSTNGTWLRLSADGQPSSIYPLTLDDEMTVIRIGTVNQYLCQLDKFSINYNEYNFCTLCHENERDALCLPCKHNSTCFKCSKNLQLCPICKMKISQQIRIFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9P8</accession>
    <name>A0A8S1N9P8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TFIIS-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0770207</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8087006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9P8"/>
    <dbReference type="OMA" id="DSNINGW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="16562" checksum="52183178111C1C99" modified="2022-10-12" version="1">MEVKNYNGWRFCPRCKNLLKPYHDGVVTEILQFKCRNQDCGDNGIIEVKIDKTLNKDALLLKKDYQAAKAIDITDNTLILDPSMPRKRVICNKCNYDEAIYFLKTDKNEKEIIIQYICANKTPICGNKWTQKEMNEPKLTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBN0</accession>
    <name>A0A8S1NBN0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0760186</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBN0"/>
    <dbReference type="OMA" id="EKCPQFH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000654">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="14"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8086425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="552" mass="65156" checksum="8C8BE2FAF8F73204" modified="2022-10-12" version="1">MSEDWKKKKIDNFVILNKILGKGTYGTVYLGYMDTGQNQVRIAVKTVPMDSVKQSPQILNLIKRESTILKAVEHPNIVRLYNANRTLNYIYIFLEFCPDGDLRKFMQGKKEKHLSELEAIIFLKHIVEGFKELFQKKIIHRDIKPENILLSNGIAKIADFGFARVMEVEMDEPGKFSRNGTPIYMSPQILRGQPFSAKCDVWSLGIVFYEMLYGKTPWQAESQIQLEQLILNKPLKFPTKPVRSQKVKELVAMMLQVEEKDRLSWQQIFENQVIKMNEDMVRQNMNEIMKEDPMTKSMSLNKIYLDQYKVVGYLHTKIEDYRDDSKNDLDGLKQMFDEENPDLILSQYQKEMKRRESFQKFYTYYLYERNVAFFINYTQLRIIKLQTFGDITLVKEQFLQLIFMLARNQLLHLDKINSALNSNKHDQFDQELWGRFIVSKDCSRLLTTIKNDILIIKDVFIQVREKCPQFQQFFDKIEDVKDFLGQYKQIISKAIEILKTASTSLGKDVYSGIYYLKICLNPYDFFKQVDFDFNAFYEETENASLEELLILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NE53</accession>
    <name>A0A8S1NE53_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0780173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NE53"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000084">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="122"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="299" mass="36278" checksum="B95E96DBEA840138" modified="2022-10-12" version="1">MWFTNSYTFIKTRIDYHIKKSHQIQKEQQEIIQQPSLNEKCKLRSVSVNSKMKINMLKDFNKISKDDQCIYNINEFNDIKYIEEVMKDDKILVKNDWQIILKNKTFTGYNFQQIHQSILLQTMEYDIRRQWWIFISNQEQNKKELKNQSYSILKKLETLHKIQIEKDVEGTIIHDMFKQYENQQSLKNILIAYANYDKELGYVQGLIIIVANLLVCYDLSVNDQQLNDFEVMDEDRDETVFLIFLYIMKNLNWRVVFLEGYEYLRLKIDVLEQMMKKKIPELHKHFYDEGIVKMNLYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NL10</accession>
    <name>A0A8S1NL10_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0840079</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NL10"/>
    <dbReference type="OMA" id="EKRQHVQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="9"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="300"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8089715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="391" mass="45300" checksum="2E7B18A23906F961" modified="2022-10-12" version="1">MSEEDEVICEQCQTVPTQFVILDCEHKFCLYCISQAIHKDDSGVPCLKCNTPTVLDHESLQAVQLTKESQSQASKTQPNYNVKPFTTEKRQYVQPIQEDLTIKGHPCLEKLNLAVERSLDKIRNIQFDKQQVKDKCQTQKNDIDTEFKALHMYLDSKQEEFHNQLSNIENTYLNELTKDEEVQQLDIEEMSIIKNEIKAILKSQDSQDIYTFKQLVATVDGIVQDKQLSIKQLNLFKQMQDELQQLSKRQAQFYQDFRRFFNILQPLQVSLVQNYASPIKVEYGGSITSDIQEKIRELKNRNSRSMNTSQLNNSRSQNSPPKNTFLNEEINISKKLSREQVIIMQQRKLGTSPMDAKTVLQSSMKHNNKSNQKFNKILNSPLTLAGFSYLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPB4</accession>
    <name>A0A8S1NPB4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0980016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="70" mass="8169" checksum="B592F0295D2F6C05" modified="2022-10-12" version="1">MSDHDDNDNDQELQEETTTNAPKFEDYSDMDKTQIPIFEKNAGTDCFKKGDYFEAXQFLFKITEQSSNYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQA5</accession>
    <name>A0A8S1NQA5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0820119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQA5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="167" mass="20042" checksum="6DCCAB260F4A1124" modified="2022-10-12" version="1">MKTFKYIIDARIFSFKQTFQLNYCSECYIPNLPVNKLLEKIMQGLILIESYSQMVSVKNLFYYLICSKHKLSNKYFKDLNMKSIYKNQNLLYSNLLNIFFQNIKLHLLIFQIQPKILAHFRRLIILQIKSEILAKIKKKLPEDTKSQVEQKINEKVKEINCYLTQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NV05</accession>
    <name>A0A8S1NV05_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0870024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8090914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NV05"/>
    <dbReference type="OMA" id="RYPIENR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="302" mass="35679" checksum="665BD7CAA08F51E3" modified="2022-10-12" version="1">MLLFPESRYPIENRKKSQTYHQESTIKELLSNSVGEAIKYKPSKPINSKLDYEENQYLANKNKQSYLQSNIFHDTKIQIRNDKQALQYQNNKKKQSSNLSSRVTTEPQYQQPSYVRGTLNQEFRKDDIKVENKQSQQSVLFNTLGKYYVEHKNPGKTNFMASDLTFLKHDSLRDKKLEYKEKQLEAEYQKQKSYLSQYNSTQSQSRRQSKQEQEINVKLNQIEIPKQEITGYDIVIEQKGKGFLPNQFRSIIKHNGFQIKQQENDHYIIQPLGGNNNIQSIAKYLKNYDNKAKIEKVPYQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P227</accession>
    <name>A0A8S1P227_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T1030064</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P227"/>
    <dbReference type="OMA" id="FPRKQLA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21742">
        <property type="entry name" value="MobB_NDR_LATS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000570">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000192">
        <property type="entry name" value="Serine/threonine-protein kinase cot-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059233">
        <property type="entry name" value="MobB_NdrA/B/Cbk1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF76">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_135, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="89"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="373"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="487"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="521"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="454"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="523"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8097125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="566" mass="66399" checksum="6561376A299E97CF" modified="2022-10-12" version="1">MDYQFPRKQLAISNSTKDKVEACKRYIERKYTDLLKAEMERREDWAKLWVKMKDMQLKAQDQEYYKQLVLKKEAELMRKKRQKFCTDDFEPIAIIGRGAFGEVRVCRIKKTKEIVAVKKMKKCEMLAKNQLAHIRAERDILSQENPWVVELKCSFQDDKYLYLVMEYMAGGDLMTLLMKKDILTEDEAKFYIAELVLAVDSIHKMNYIHRDLKPDNILIDRKGHLKLSDFGLCKYSEIKPKVELGRKIPNQNPAPTPILNKMQKRRQLALSTVGTPDYIAPEVFTQQGYTETVDWWSVGVMLYEMVVGYPPFFSDDPQTTCQKILHWQSSFQIPQDISLSPQCIDLIRRLVADHTERLGINGVSEIKIHPFFQGVDWKRIREKITPYQPQVNHEIDTQNFEQFEEEEPWNWNSSRRVKKDHFQGYSFNRQVQKEQSPVRVALENIDKVEFPKRSQPIQQPQSQIQQPGSPSHRKVIAKPIAYAYNKSARSNSPVNKNQQQQSSLKTIPQQQTLLNILSQYKRNSNNNNNSNNNSTLNHSSSRLPNLKSVREQSPRSPRNAQKYYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P4L0</accession>
    <name>A0A8S1P4L0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Calpain family cysteine protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T1050049</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P4L0"/>
    <dbReference type="OMA" id="GIGKWIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000010">
        <property type="entry name" value="Calpain 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF379">
        <property type="entry name" value="CALPAIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="190"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Calpain catalytic" evidence="12">
        <location>
            <begin position="320"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="532"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8097814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="832" mass="97818" checksum="FEEF2466EFECD989" modified="2022-10-12" version="1">MNQKLVNASIMLILQDLCRYCKQTKKNPRDLINCYVQPSKKGMDVYGFLEMVKQILPFAQNEMIEKTFSFIQQRFECQQINGQIFEELMKKIESQMTNQELFQQINFKTNCQNVSEQQKNIQISNQSIQVNQIQNQHEKKPIFDQNFKQSDYSNNNPVQLLKQNISNQVGQKCNRSEIELIKTFSTKLNNANISIIDVFNKYDNNKDHMMNQLEFQKMIQIVIKDINEYELKTLTQWVFKEQNKELTTFQFKKICEFQEQVIPVVPQSNIMESNYFENPANQKILQSNYQFQEFYVSDYNLVAMKEFVQKYQQLKSQNQLYIDPVFPPNQQSLGARFQNFQWKRIPEFIQNPKFFVKENTINRFGIGKWITPDDLQQGQLGDCYFLASISSLGNRRPDLLLETFVTRTFNPQGLYGVKLCIDGEWKVIGIDDYIPTYYNKPAFTRGKDQEIWVMVIEKAWAKVFGSYENIEAGYPSEVLRTLTGAPTRILFTNDEKFIEDLESCIKNRCIMVCSSKNENKQQYKLMGLISGHAYTVLKIKDVNANTKLIKLRNPWGKEEWKGDWSNQSQMWTPEQKIQFKITNNNDGVFYMSIKDFLKYFDDLSVCYVKEGYQYQSIIVQSNNKKSEYFSIQIQKSGCYYFTINQKNVRFENSNNYSPTQILLFKGDCLINGNFKCDRECWLSSDLEQGLYTLVIKTQWKFSKTNKYTLSSYGPAQVNINKINKIPNLLQQAFLQLARKSIKKKGYQKHPEIKQAYEFHLKYGYGFYYVENLSGKSFESKVKFSQMDGLKLRKPYRGNNLEIKLSENQEFLALLSVSNSGYAFGVEEQYVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6Q6</accession>
    <name>A0A8S1P6Q6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1070196</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="TMERIAW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19670">
        <property type="entry name" value="UBR-box_UBR1_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.30:FF:000002">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase ubr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38924">
        <property type="entry name" value="ASPARAGINE AND ASPARTATE RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38924:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="11">
        <location>
            <begin position="49"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="10">
        <location>
            <begin position="49"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8098749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1433" mass="170530" checksum="18D6AE308499C79F" modified="2022-10-12" version="1">MNKFLKKWTEADTYINDNIQNIQRELLAVILPNDQSFSNWENSKTKFQSTCGYFISDQELSFQCFTCGKEPTHMICKNCFIPKLHRDHQCIIDVKNKGLCDCGVKSLILDDGFCSKHTDKININKEKEISKIPINIQNNITFIFQELINSFRSSIKEIKKMNVSNLQTTLLTLYHFAKQFKDQNSIQYMEMYQKNEQISELFRRIANINTLIFTTIELLIDKRQNYQLLIQQILSNKNLINSKYTILEKLFRYQIYIEVYQKGYDYKIDQILYKLFNDETFKAYIFSIILKNFSNFWLISQFKTTIIDEKIEELFKKIDQSMIQNQKTYFCCQTIVQYKVQNIDLYSQYRRIQKQIQPLIKHLISNRQLFHSVKTSKILISESQFQSFEMQEQFFSQNQNIQDFVVTIQQLHYTSGALCSDNQLILPQLFKKRFEYKNFGEIALGQLQNILNKNFDSFKQIDINIDIIKVFNFNKFAITNLILSLGKGIQMRNKTNLLNNSIILADTIYYLQDQATYYTAIYTGLMNLFSNQYSNEILEKNIIKLLFYQTYFILKKTININQINIKPSQSLIQDLPTIKNGMIIQKLFISYLSYLYCVTQFENGKQFLHYLIDILDEDQQNFEKILNQLLKETVYVHLIIQENQDQQIKSVYQGNNNHTEYSQFHRVDTCLIKLYIFLFDVNGFSSVKNMMEEFYQIQNGTILQELKKEKYQNFYQMFINLILTDYDLYNVCCPLLTQLTNELKLSLVRILGNYFNISTFIEYGDIQEKLKNFGVLITSNFSNHILQICEVDQTSKKLKLKGEYKIFYEPCLIQNQSQLQTQIEERLFEQKKSDSEILLGNGITWDIEQFSNKSYRVLMQQLLKIYCHFELFLRNLIYLMKKDKLIVQISYLIYAQLAYLNQFDQQLFKQYFEIVIAQLEEIQQIINVKQKQKQQIKVLIQSINELNQKQIMNNMQKQIKQKFQTLKEQYKSKFNKIQSSNLVQKMINEKEENQEEDLCFTCKLDVTTENSVGMMYIYWKSQTEFFDETHQMLKDQLLFDFDLGIQTCNHYFHDKCLTQAFDCYQIMDQEYGQGFNQYKFYCPICKLHTNCRFPISKIDKRIVKSLNSDLIFIYSLINMDDEFEDELPQQINLVKIYINLFYDLLVSLFINPQNYKKAQKNILFNQLFCCFYETIQDMNQQDIEAQPQLQNIQQKNNFLVNILTSMYEMSVKQCSLNQLRLSIIELISQYSQLNKEEISLLISSFGIEEQMMVQELNQKNKQCIHDTSLEYYQNFQIKTSQYIQKTLGETFLQFHSKYFCNKCSICKFYNLKKSKNSGVCVCLLCKDLFCNESCSLSIKDHLNYHVEKRHHGNSLFVSLQDGSVTIISQSTSIKKFKYLYYNNLGEKINIENPNSDWNQYILDITKANELVDIIVNNKYRKIIKSQQKRSNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7S8</accession>
    <name>A0A8S1P7S8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1080071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7S8"/>
    <dbReference type="OMA" id="RWQSLEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="39035" checksum="A0E69B82CA8948E8" modified="2022-10-12" version="1">MGNNCTCWKHDLQNQIEQDKKSKPRWQSLEFLKSQEHKIVKMQSWIRGWQARQRVWKIQLQKYNDSVNEQLRTYSVSVINVGSRQLPPFDFGIDDINDSLVHQKVLKAPMKLENGATYFGEWINDLRHGKGILICDDGSKYEGYFYQGNAHGRGRLIHSNGEIYEGQWENDEAHGQGTYIHEDGATYIGQWEHDLQQGKGSEKWPDGSYFEGTYKQGKKEGLGKFVWADGAIYDGEFRSNNIDGYGKYTWPDGKQYIGYWQNNKKNGKGKYIWPDGKVYEGDFVFDQKHGQGILRFPDGRVYEGEWQQDKQHGRAILKLPNGKTSYGEWKNGIRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBU0</accession>
    <name>A0A8S1PBU0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">26S proteasome non-ATPase regulatory subunit 2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1120033</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PBU0"/>
    <dbReference type="OMA" id="LNYRMIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016643">
        <property type="entry name" value="26S_Psome_Rpn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041433">
        <property type="entry name" value="RPN1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040892">
        <property type="entry name" value="RPN1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18051">
        <property type="entry name" value="RPN1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17781">
        <property type="entry name" value="RPN1_RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015965">
        <property type="entry name" value="26S_Psome_Rpn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RPN1 N-terminal" evidence="3">
        <location>
            <begin position="42"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit RPN1 C-terminal" evidence="4">
        <location>
            <begin position="808"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8100291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="878" mass="98219" checksum="D3C720867BF73C27" modified="2022-10-12" version="1">MEEKKVSLDKQAQKKPGKQVVQLSEQDLELKNRLEQYVEEIIQNNPESLEKLKTEVRSATTSMTSVPKPFKFLKESYGKLVEFYNGLETSRFKKQLADFLSVLAMTYGGDRDSLLYLQEGTLEEFKFWGHEYLSHLAANIGSEFQIRLQKQEGADDLLFLVDEIIPYFMDHNSEHDAIDLLSEVDQLQKIEQFVNQVNIQRVLVYLLSIVPFCSDQDELDTLLVTAFNICMKVNEYTNALRIALRIDKPGKVEQVFEACQDAIIKKQLAFQLARHRYQIDEGIINDETLKKIAANQYLSQFYINLAKDLEVLEPKTPDQVYKAHLEEKNKEANFEQIQKNLAATYCNGFVNAGFKKDALMGNKENPWLNNVKNDHTAIAAVASIGLINLWNIDNGTEQISEYFDLSDIYTRAGGCIGFGLFCSGIVDENQPAQAMLLAQLESPEVQVKIGAVMGLGLAYAGSARVELQEQLISVLIETADSVELAAMAALSLGLIFVGKCNDEVANAIVQTMLERPESQLDISISRYFAVGLGLLYLGQAEAADIVIESLAVIPHKISKYAKVTLTSLAYASTGNVLKVQELLGYCSEVTSDDKGTKIDKNYELQQVAILGIALIASSEDVGSEMVYRIFQHILQFSDVEVKRAIPLAVALINISNPKIQPMDLLLKLAHDRDQELSYRATLSMGLLGAGTNNSRIADKLRGLAVYFMNDSNGLFLVRIAQGLLHMGKGLLTLQPYYSDKFLMNKVAIAGIISFLHGCLDIKNLILEKNHTLLYYLGIAMYPRMFFTLNDNLENLPVQVRVGQAVDAVGQAGKPKRITGFQTHTSPVIVSAGERAELGNDEYMPVQDMILENFVILKKNPDYKPEIEQPQRKKTSFSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCD3</accession>
    <name>A0A8S1PCD3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1130103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="VYLMAGF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="752"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="944"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="967"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1064"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1296"/>
            <end position="1318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1356" mass="158083" checksum="747618662AF5B431" modified="2022-10-12" version="1">MTNSQNKQLINVLVYCNYISNHAPLKALIKLILQQADNIYFKQSFNNLSLELYQRAKQYQREAIHKNTIRSTNEHQSSLNVQSAYMTSHSSYILFPILIETIQSKINFWSKLINGYQDIDHYLLESLKTTQKMLLCKKEIEQRFDMINNKLKSQQQPDILTLRIIQIYYSGIYSNAFQAYQIEKSIEELIKNERYKQDETLDNMQLIQNRLIILKSSLVRKRGELINLNVRQLALFLNENEDSLKGIKHCNQLMPNFISSIHEKLMDNYLQRGYSKLMINGEATFCQNITGYIEQCSLNLFNFFDDKDDFILNMIITKQQSNNEIILFGIDGKILGFTKYFYEEVLQNNVKMNLSHQTTKDQNVLKITVKEFLNKNPLIQFYIPGIANQVQELRQQIVSSSNYLMNNLKSFWIIPNNHIECLQNSQLIFSQFKKRSYGSQQNFRSYKSQTYSKYSYNSINTQFDMQYDDSEMPSELKSNISINGIPILILHPEVEQQISKLVEITDSLSKIPQTGVFYSLQFKTLNFKRGNYGYFMLTIKDFKTIANQTINNQQTSRTQQSLYQTLIPSQEFSISNTKSPNSFFQDIRSENPDDSQYHAIQEIKQMNNNLKLNESSAAADNSFKFQLKQSDRSNLFDSSRQPLFARPKILEIDINEEVEQIELDMQVIKEIKQDQIEDEQDEGEKKVQSVSQSQKKSSNILLKFQTKQIKNKENDFASNPSRTSTNSTSKESLLIVQQLYHNTQLITPLKKIAVLLFIICSGILISNIINVQFISENLISQTNQLATLRQPQNINYFYTSEILQEWYLILIKNKLITLSPFMFQRNLDTLQGMYDYARTTQVNLAIIVPRLSMEQNLQTIMQTKFYQENSIVTIEYPLEEFYSILYQNTEISYRKFINNGSIDFPQLMPQGVVRLNLLDMLDFHNQLIEVIIENTIDKQTQVKSYFLSVMFGEMFSIFLIIGIQLKYWLFIDHIVKSILFLISRLNENQALDQMNRLSLIKEQLNNQINGNWKLFNFGDVMYGFSEKKAKKVILKSNVEIGQNNIKATSALYSRIQRTYYLNRINAFITFIITILLTVYLMAGFLLHMAKNDNFQPSLTVTLKFVQVRHNMDSLTLLAGLIKSEPIIPNLNLEYLNQTKACQLLIEYRDNLSPLINEVAQVILENANANNVKSRFDTILNYDLCLSSTVDSMPICDLSTIKLQYDQKDNYKSIISNGALGYTAALIKFINQEFDYEIQNLKYNVNETNKAVIQQQSFQNFILQYFTDIQTIMRQFLVLFQEDNLAIAQGIISVVQVYYYGVGTCLYLSYIILSIIWVYKSQMKIQSLRQVLVLIPPDHILTTNVRSQAKSIHQMLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PFU6</accession>
    <name>A0A8S1PFU6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peroxin-7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1170065</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8101975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PFU6"/>
    <dbReference type="OMA" id="FAVHWNL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005053">
        <property type="term" value="F:peroxisome matrix targeting signal-2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044536">
        <property type="entry name" value="PEX7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027:SF1">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="147"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="192"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="235"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8101975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="36006" checksum="9674DF8828B9F620" modified="2022-10-12" version="1">MQQQIPPSFFVSNLSCMSAKFSPFDIDKIAITCADNFGIQGKGRLIIGQIQPNQPIKILFQSEESDAVFDCSWNEADPNVIAIGCGNGIVKVLNAGTQQILTQYLESKEEIQSVEFGHKNPNWLLASNLIGITRLFDITAQKPVAYFQTHKGCAYTCTWHPIQQNIFATTGNDGAMRLWDLNSPSNKNIASIKAHMSDTLSCDFNKYEELIATSSADKTIKLWDLRNLKAPIQTLLGHRHPVRKVKFSPHEAIILGSASYDMSVMIWNIQEPSNPLIKNHPKHTEFVVGLDFSIHTEKQICSASWDGKAMIWQWDQEQPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGU9</accession>
    <name>A0A8S1PGU9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1180064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGU9"/>
    <dbReference type="OMA" id="MKMRNAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="178" mass="21067" checksum="01D5E3DE9F18C8D9" modified="2022-10-12" version="1">MFLFQKRLMTLSQSSQQPIRLKPKFYSQQKENMPVQKDLPKVNLINKLNEASKITKPQPKQQVQITQKTISKDVWHKKSISMKMRNANFKDGKKIISGQLIKYKKGDQIYAFRCIKDTMKQCKLKDIKQDHDVDTDDEQIFMATKNMFMSLCQSIKRELFNNEDNQNENKIIIDDDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PI89</accession>
    <name>A0A8S1PI89_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1190036</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PI89"/>
    <dbReference type="OMA" id="NHKGPNG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000275">
        <property type="entry name" value="SRSF protein kinase 2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF9">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="69"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="275"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="295"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8102699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="665" mass="78332" checksum="9EB35C4E37AA2D4D" modified="2022-10-12" version="1">MIQSNTYQQFEAQMQSSQGFKNEKPMNEQIEELMDHLDHEHDSEDEGMEDYKIGGYHPVHVGEVLLNRYVVIQKLGWGHFSTVWLAKDFKYDTYVALKIQKSASHYLEAAYDEVEILQKVAQNVQNPVWIQSLKEYYADQGRTSFNRDDTHTVQLLNSFVYKGPYGHHFCMVFEILGVNLLEVIKRFEYKGCPMDIARRMAKQILIGLDYLHRICGVIHTDLKPENVLLCLSDEEIKDIVENGQLTSNQLFSDRIHIYRQMLGIVEEKPVVDDKKTVQKQEEDDLDTQSTNLTKTQKRKLLRKKKQQQQQQQQQNEFINVENEQTEPEKPKSIKELFQQQKKISFTQQKKLPDNFRLKIADLGNACWIHHHFSTLIQTRQYRSPEVLLGIKYNPTADIWSFACMIFEMLTGDYLFEPRQGPNFSKNEDHLAQIQELLGKFPYEYCTRGAKAKRYFMNNGQMKRIPQLHFWSLFNVLTEKYRFKQEEALSFASFMMPMLHQLPEYRTTAQEVLKRELCSNNLDDFQRRAQLKKYIDQIFAYQNFRNNIPNSGWLQAKTDSQIGHKMNDEEFQKYNKQKKANEEVEQFDQNIEGIQPTLLSNYRRASTRSYDGSISQTPQQDKVIRKEGKYVDHRVIDRSFTDLGYIGYGDGIDLEQLDSTGNWQFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLS1</accession>
    <name>A0A8S1PLS1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1230050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLS1"/>
    <dbReference type="OMA" id="YNQRAVQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="328" mass="37960" checksum="471A4CD6DB0E3799" modified="2022-10-12" version="1">MSEQQKYIGNILSRTKSLLTTNYQKLPLKAPKFVENIIVYADTITQPLVQKVTDTVDDNINRSLQIVQSYEKNLNIAEQGQKLKQKGKELTDQLRENSKKALDQGVDLAKNNGATQFLLHTCDNVANKVFSSIEGNVTKLQKAILKETKETNSQQTYNQRAVQLAHNLTELTQGLFTLLNNEIHTFNVQKVQQFKIYVTLNLKQHLNAILFTYRVIVIQPISSLYTTSLNELKCQLGVLREKYNNQELLQYIIEQLTQLKEYIETIQFEEFQALLFTALKQLNLFKILKSQYQKTIQTYQNSLVEQRKVIEEQVNQPEQENGETQINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMP8</accession>
    <name>A0A8S1PMP8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1240066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMP8"/>
    <dbReference type="OMA" id="QSHHGAF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="55882" checksum="EAE566265AE6F27E" modified="2022-10-12" version="1">MFFINQYIERDEESIQMKWILRFMKYYYFLMNKNSPSPSSLTPVILVLVTNKQGKVLEHSQFFRTFSSFGQVKKILIFERKIIWKVFIEYENAQQAQSALVLDGTLFDYQLKMRVHLSQRDSLVFQNNNNCGVDYTTLQSKQQFFQQLKEDSGSLNQQLQQNLDMLNQLIAQLSQTTDLKEQISLIQQIQEYRNQTQQLTVLYQQQLRNYASVLQREGKKQKQIVSTVTTPSPSPFLHSTLDSIRQQIPEESSEDDKEEFSDGESISSQSSDKSQKSTHSEDNYNEVFCNDSTTPFSCGASPFTPYPQKVITSPFIMTPTTLPTYLIVQHSNINLRVVYNIFSTFTRVDAIYQSHHGAFLQLASKEEAIRMKNLLGKALLFGVPMNLIVTDQLSLDARQVTLPPNERKPLLQQQLSNSILITGLNGITIEQIYQYFGCITPILNMKFINQSSCKILYSDVGCSLSVLGYIQDAKINGRSVQLSFTAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2Q5</accession>
    <name>A0A8S1Q2Q5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1410017</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2Q5"/>
    <dbReference type="OMA" id="EFRVDKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005878">
        <property type="entry name" value="Ribosom_uL1_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01169">
        <property type="entry name" value="rplA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8109517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="246" mass="27003" checksum="5CA57ABC9F1DC0A3" modified="2022-10-12" version="1">MLLRGRILAYFAKVKVTDTPKIRIGEAVKLIKESAKAKFDETIDFQLRLNVDPKHGDQNVRGTCMLPGGLGKNVSIAVLTSNELNDAALRAGADFVGQEEILKSIKDGTYKFEKLITTQDMLVLLKPYARLLGTKGLMPNPKAGTLVLPQELTQAIKGAKAGSIEFRVDSGSNLMVPVGKKSFDDKLILMNIKAFSLALNQKRPTSLKGQLIGEARIKTTMGQPVEVDVSSFDQRNKENDLKDLVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5I0</accession>
    <name>A0A8S1Q5I0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1440088</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5I0"/>
    <dbReference type="OMA" id="CAPVNRD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003958">
        <property type="term" value="F:NADPH-hemoprotein reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06207">
        <property type="entry name" value="CyPoR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000023">
        <property type="entry name" value="NADPH-cytochrome P450 reductase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF17">
        <property type="entry name" value="NADPH--CYTOCHROME P450 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="2">
        <location>
            <begin position="11"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="3">
        <location>
            <begin position="201"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8110497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="582" mass="66759" checksum="3CF28166D744342F" modified="2022-10-12" version="1">MQQTTDTREIINVYYGTTSGNSSRLAFQFADEAIQHKFIPKIFNLKEFNPDVFIQTRLNIFFVSTYGVGGPTSDAIDFNNWLQSKDRKQNELQGVHFSVFALGNTKHEHFCGMGVKTDARLEELGATRIFQLGKGNSCDETTDNDYSVWVESGLFAALESLSPLITVPANQVQSSIYKIKEFDNIDVLIGETLSFQAQKYKDSYSLKIIEIKELRKAPSAGNSTLFLSLESQNIPYKAAQNIAIYPENTEEYIQEICSMLNLDPKFIFQIETKGNHPFPTPISVHNYLKKYCDFLGPITKKQLYTLSLLVDDPKVKEELKFVSSHKGREQYDTNYLQTRECFFNLVKKYNIKNIPLQQLIELCPLISPRYFTIASSPLKYPNKIEIIASELLITKERLGLCSQFLRNVKVGQEIKCYLQESAFTLPQNPQAPILLLGIGAGLAPMRAFIQEKDEFIEQNKLDQSPFKGPMTLFFGCRTMNDYLFKEELEEYEKNGTLNQLKVAFSREGQQIRITNYLDVEFLNQFLESGGIIYVCGSTQMGRDVQIAIENMFKQIRKVMPYLAYRKVNELEEQKIFVKELWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q665</accession>
    <name>A0A8S1Q665_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1450036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q665"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="76" mass="9202" checksum="260938536EAADED5" modified="2022-10-12" version="1">MQQFSKLGKKESYNQFKLILNDSYKMNTRVMESSIMKQLTEFEDRYGMFKQIENSNRFISNRINDLVYDIKTVNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q739</accession>
    <name>A0A8S1Q739_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1440130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q739"/>
    <dbReference type="OMA" id="ETERFNN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="33591" checksum="B2FC3A703DBB77D1" modified="2022-10-12" version="1">METEYNSKKKPLVNSQGPSFQSFFWKSYVEFDQDDSEITLKELIPFLLKMTYVQALNLIFVYIIAFLTFYLESFQNLLINNNSDFVYQTFVLAFIILFFIILCTRKIKNMIIKLILLIILSLVVIILFGNILGLIVIYFPKESTIMFLVYILLTSFTMLISFIMVLKIVSPEKFTFEKLVAASTLSQLILFIIFISIYSQEWLFIVIIWIFHYLYCLCFVYELLQISTGYNKIIKIQGNNTVDYLMAGLIVYVGTTCLSIDFVPIITLFLIFESMSIMFCKTDEKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9M8</accession>
    <name>A0A8S1Q9M8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1510012</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000156">
        <property type="protein sequence ID" value="CAD8112312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9M8"/>
    <dbReference type="OMA" id="FIRIMIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01610">
        <property type="entry name" value="PAP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF28">
        <property type="entry name" value="DIHYDROSPHINGOSINE 1-PHOSPHATE PHOSPHATASE LCB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="353"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="79"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8112312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="410" mass="48050" checksum="043C2F844320860B" modified="2022-10-12" version="1">MKFNSKKNAIIGIILFCIYIIVDLFIDEILLQYTYKLIFSIKNPLFIEFSYLIKTTSLLLCEIIFLYLYLSFNKKLDVLLLILFSCVLTGTHAFLKEIYNQGRPYMFNSDVQTLECIAELGKPSGHAICIVSYMISLYQADRFENEEEMKLPLFKSILYTIIALIVCLSRIILGVHSIGQVIYGIIFTLCMYMIYLNIVHQQIKKFIHKHIKYQSHKLTIFTAVLFLVFLTLSLSADFYSRNYFAQQPDLYYKYIDAVQECRVRNGLEKLNDESGFILQRSTSHSYGYYFIIIGALLGLVIFRGVYEEGLYEVQSKFRTRLTLFIRIMIFYLMIFSVQYGMEYFEPKQYESYLVMIVVGHIILGLCVTLIYPILMYFLGLEIYGSMQCVNKSTLFIDYSHIWESDSIIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QAX6</accession>
    <name>A0A8S1QAX6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1530017</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QAX6"/>
    <dbReference type="OMA" id="CNRTSLW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF14">
        <property type="entry name" value="CYTOCHROME B5 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="5">
        <location>
            <begin position="5"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="5">
        <location>
            <begin position="105"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8112836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="215" mass="23709" checksum="772C89124FFB01C1" modified="2022-10-12" version="1">MNGEKRIVQWNELADHCNRTSLWVAIEGQVFDVTTYLAEHPGGDDVLLKYGGLDGTQKFLEVNHSNYARSLRNARLVGTLTTDPQPNDYIKLVKSKKQKVNVNPNRQISWEELGQHNKKEDLWMVIEGKVYDVTDFQDDHPGGPAILLGKAGDDASAAFHDANHSQSAYRQLEKLQVGVITGVKPNVSGSGSSTNIIFLILLILAIGAGIFVITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDW6</accession>
    <name>A0A8S1QDW6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1570051</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000162">
        <property type="protein sequence ID" value="CAD8113908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDW6"/>
    <dbReference type="OMA" id="PNTGMKK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001567">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001352">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033931">
        <property type="entry name" value="PDK1-typ_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14593">
        <property type="entry name" value="PH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="7"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8113908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="438" mass="50949" checksum="5114BA55E744C108" modified="2022-10-12" version="1">MQTLNDYEIIEKLGSGSYGDVMLARQKENGQLVAIKTLEKRLLIKEKKQYQVFIEKEVLTQVKHPGLINLIASFQSSSQIYLVLEFVEGGDFANFLKINKNISQQNIVFYSAEIVSILEQLHSHGIAHRDVKPENIMVANNLHIKMIDFGTANFFDERGLPESVRDKLNELREVSQQDERFMDEIDQYQQKHKATFVGTAEYVSPELLEDDVCGPQADLWALGCIIYKMFTGITPFCDQTEYLVFQKVRACQYQKNNKIPQEAIDLISKLLVRDPLSRLGGGLPNSKNTYRELKSHPFFKEINWDQLWSRKGPDDVQIHLKKQSQKQELQLKKQSSALKPEVILTGLVNKKTGWMIYKLRNMILYDYEIPKLEYFDPNTGMKKGQIILDKNVIIELLKGQFNIDVPNKKKYYFKECEHPAQLWVDKIKDIMKKRLNYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QE63</accession>
    <name>A0A8S1QE63_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1530089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QE63"/>
    <dbReference type="OMA" id="QFYKAKA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000413">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="80"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="170"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="255"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8112955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="691" mass="81005" checksum="457D3225BE7D45F7" modified="2022-10-12" version="1">MNRRKTVAKDTIIDVEDQIKQKELLFAPVIQVYRDNLNLTDIQVDSITGIMQNIEIFKTWCPESSTLFEFTRLICQNLQYERIEKENAVFHIGEQGDKFYIILTGRAGVYIRRQQQQIEAEESAMLPKIEKILERMQIKTIDEIEQDHKLSFYEKLIKKQSKPVKQIEAELLLLYVGNFDMYFTINGICKFQQLSQVHSGLYFGDMALTTDKPRAASIIAVTDVQALSLNKANFKKIFEKQIKTSQEKIDYFLKMFPTMTKFKMSKLIMYFTQYKYPVNYTIWKQNDIVDGFFLLKDGEIQLQQTVDFNPLLKSDQNQLQLSPKKEKSNQKELITIAHLTGGCFVGETDIYLQNEKRDYTVKTLTQCNVFTLQLDNFAIVKKSFPEFIIPLQTLQQKNIQLYRKRLDEIVQTKIANVNLHKKEEIKNIERRYINEIEIKQKTQNSFQEQSSPILRSTLQPKMTKQQMVEQNLSIADQHTKSDIAAGKNEEFNMLKMAGENFQKCLLIRVEKQFEQFQPAKPKGKTPYFSKHQKDIKDLLEQIRRHQLPSVQQKEPIVFNQQQEEDFQNNLPFLTMTKQQLRVINPLIQQKVEILKQCLSRQSHTSISKSKSLCQTADQFYKAKAQQGFILCDSLFPYKPVHSTKNSNNQHYSNFKVVQRNKTTQQSQIQKRSCQDITDTNFFSSQPTVQFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFA3</accession>
    <name>A0A8S1QFA3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1600107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFA3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="68" mass="8458" checksum="CED4E50C4BD17871" modified="2022-10-12" version="1">MQYPEFKQYSGISYIYLNLINNINFYTQRIQNQRIRYYQKEQEKSAGQQICLMKFQEYNLFSRINLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QG64</accession>
    <name>A0A8S1QG64_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1580078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000163">
        <property type="protein sequence ID" value="CAD8114194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="EYHMENI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035777126" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1041"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1041" mass="121774" checksum="066CF81B487FE296" modified="2022-10-12" version="1" fragment="single">MIILIFVINVLKICSENVTELAIALYPTVGEFYEYHMENILPSSQLNCKMHPQIPNVKVVNQYEEIYKIQGNSYISMSSNNTHFGTLSNNNEVIIYEWKNQMIQQVGLSMSIDILYNCFNINLFFNYTILVDCYYNNEILFIQLIDGQSIIAYLNQSNLPTSTKIQSIVNGTNTFIVYAQYFKNYSILTLFSSSFFNLSSLQNQFVDFDIPITISPNIYAITSSELYQFSISSDSQFNFMYNFSDLGYMNNFTTINVYYNFQNYSQCDQILLLFNKGQKNSSEFKVAKLFGCESQIMLQNNNCPFNNCQFIENILQNAEFIIYKSINETAILFQEKTQTYYFYQNNQNNQKVYLNNDNELFQFDKEIVVYQISQPSIQINLTNSESQLNQSFFNLMCFNYNKTYNISLININLQILSQQDTNIYVMYNQGFANLQLWQRYSQIVGFYGYSGQLLHYTLNKNEPYFNFNPITFSNFGKIDQTYNLIQILSLMFQTAQRYLIGYNNQQLDISICTFNFNNYSYQLKLLNSINISINASSLQVAYTIYPFMLIIGLSDNNNISLVQYYNYKNSIITSNYTFEQQFSDFLLTYNSIIILILLQEIQILTFNYTVTYTLNQQSINKLFNNIQFNPIQIVVNTQSQSSFLYINNINEVIIISIDQNSFPIPISLIQVNFTIKQINLVNQQLIISYICNNQRNICFQVWNVQNLPKYYYVKNLLRVNFNNNITISSDNLFFYVTFSNYTVYVYNPLLPYHMSLYYMLELTSPIQCTEQYSLSESYSYGKSIILSNNNLYTLSEEQFFNLTYSQSDYVFNNSISYPQLTFNYTVTSALNNTAFQQTSNQTAYFYTNFTLFQNQICPPVYLSEDNIIPNSKNFSYPMNVILDRQVGYCNLNKTLALNRYCSLTQLLNQPSNISNIQNYSLITSINNKYFALQNNSYIQSLSSNLNNLSNFNYANHNFSHCLQSTSYIYSLYTICQNITSQYLLNFTLNSSGYIVNLNTTQLLQTFYNISKINSILNQIFLLGILQNSQPQQQQLYWFNQX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QHN7</accession>
    <name>A0A8S1QHN7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1610046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QHN7"/>
    <dbReference type="OMA" id="TRIVFMR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8114734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="650" mass="76463" checksum="85CD747AFB611DF5" modified="2022-10-12" version="1">MSQITQIIRKMDIFGQPINLNLDLNTKYKSSFGGLISIFIILLLIIYSVNEIIGYTIQRGIQITQETKFSYDPDLLELNQTNFIFAIRVNQQDFYNSPTFDIIINQRQQKNNLRLQLQQCTLEQFTSLINSDKILEFLQSNQMETWLCPTSQFSIQLEGTQYSLSFKNLIIDVRKCEELSSWGKTCKELEENESYQLQFITKNTFINPFNNEFVAEDYLDNSMSLQLNPGDLQRIDYHFDKHITKDFQGQLKIEGLLDEENLSVFSTNSYRQLYEGKNDSIWTRIVFMRSNFSTNYNREYQTITDLLSKIGGLSQSVISIVGVLITYYNRTQFYLEISNRLYDYDFNTDSDQGTKGSGLVDPFHISSQTQQEGGTKTKKRKLDEIKQNEEKQDSMEEDPQYKERVMKVVEEHKKIMEKYKFKSRKHFLLSTFQQTIMTKKSINLSLKYFLYSFCCKKANYKQDSQLFLLQKSLNSVRQELDVFSILNRIHQVEKLKQILLDRDQLILFNYTPKPVIGINEKDKETNFLHYSKQLSLKQMSLIEVAEQIKQQLKSSFLTKEHLSIESVFNSYKAIAAKNRKSNRYYYKINRKLKQFLGDQVKALFKASKILKFPEDDQIFQISDLLSIGEEMAIQIPLQLKPQMNINQQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QIA5</accession>
    <name>A0A8S1QIA5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1590029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000164">
        <property type="protein sequence ID" value="CAD8114270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QIA5"/>
    <dbReference type="OMA" id="QFPQHQV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="393"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="685" mass="81400" checksum="1A3EB7712C6CF98E" modified="2022-10-12" version="1">MSEESNSILTTIKYYKSMLKFPSFPKQYSELDETQEQNELFFSNSKQRKEESLKLLKEWAQNRMELFERNREIVEELANSCDYKIRLSVDFLQIVYKFFNEKYFHEQQYAQFLITKPQHALKQNPLNQQLYSTVGTSISTFEEYNLKRLEKIANFAHAIQTEILKAQSFSELSDMDRQLDLLLSSIITQKKLLQNYITSAGQKLNLIIQQFPQHQVPQRQRKPIFDDQQQQQQQQQQQQQQQQQQQPQQAQGKTDKIQQNISAIGTLLVGSSSLQDPVEMELNQQMKEQERQIREQQLKEEQSLGNGNELQQEKQEQQENQEQQEKQEKQELQEKQEQQQQEKQEKQEQEQQQQEQQVQTNKEVEQQLNKQIQNQQEQLEQIDKPEQLKENQEQNLQQNQQDKQPQNLGEQLSSAEYYKQQQQQKERQQFDQNKLKSKNYQSQGQPNIQQQIKNEDDGRKQNNQQVDVYRLIISFVATQQYANKLLNQLAEKVIQYWKQVIAIEQKRAQWAKDSCLIYKKGIAEVHNIQVELPELKIDIEDYYNLKKLLPKEISQFEDGHQEEFLQTLVIKEMGCQAARLLLIKEFRVHIIDKTNELQAILVLTIDRYVGCWLKHDETIEAVDNPVLHYPINDMVIQKVNELVLDIMPSRHLLFLNIANDRQKSKIKFQTSDDMEEFCTISQAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QRY7</accession>
    <name>A0A8S1QRY7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T2440001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000253">
        <property type="protein sequence ID" value="CAD8118516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QRY7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8118516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="404" mass="47221" checksum="3DB9F2FDB3347924" modified="2022-10-12" version="1">MLLIKCQFGKCIQCSFKYELINDKCYYICDGYENQEDQENRNCQNRINNLIENGYYQHNLFNNENSKYTLASSLTCNLKDFGIFGYFYNQCRIPEIQHCKISFINICLQCEENYQLEKNRLRCTSICNDGIVIEQQQEQCDDQNNIQFDGCYKCQYSCQLECINCVENKCYQCFDGWKLIDYGCYQYCGDGQIAVTSMEQCDDGNYDNGDGCFECKYQCIPYCNACIDKNTCYICEQYQELYNNQCRPICGDEHIVIGLEECEDGNDIPYDGCFNCQFQCEEGCQTCTFGICLECIKGYTIIKDICEVENYTHNFDEEEQEVSNKCGDAIYTDNEKCDDGNEFDGDGCSSLCLIEPNWFCNNQPRQSSFCSYNTVFKLQYLNYTQQIQYVQLSFLIKLNQMKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QTS1</accession>
    <name>A0A8S1QTS1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2200005</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T2540002</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000229">
        <property type="protein sequence ID" value="CAD8118017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000263">
        <property type="protein sequence ID" value="CAD8118639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QTS1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036273298" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8118017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8118639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="42" mass="4800" checksum="7D1CDD609782F855" modified="2022-10-12" version="1">MCSSVNVLKFLLQFSIGLLNAMQKSKVIVSANKYRSWHTNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JM95</accession>
    <name>A0A8S1JM95_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0040547</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JM95"/>
    <dbReference type="OMA" id="FKCEEES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="48"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8043323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="106" mass="12453" checksum="A0E72511DD92B06A" modified="2022-10-12" version="1">MSQQQDPPSAKKRIRRCTKHDETPRPYKCGCGRGYYSYPALYTHLKIKHEGQPPTGTQIPSDKHYGSRGRPREKNLKCEEESKHQVFIDEDKREDNHKDEKITINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMG8</accession>
    <name>A0A8S1JMG8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040538</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMG8"/>
    <dbReference type="OMA" id="NYHQEIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="110" mass="13003" checksum="217C33F31A315ADF" modified="2022-10-12" version="1">MLSKRKVSSQNLEESSNSIEQLCNKIRKVIVSEVGEKYIQNHSEKDIKQKRTKHKSKREDEQYIIEIIQNDPVEVEEQLSSIQAISESQSWVVEELFDEINKLNYHQEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMX9</accession>
    <name>A0A8S1JMX9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMX9"/>
    <dbReference type="OMA" id="DEWIDTQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071818">
        <property type="term" value="C:BAT3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20104">
        <property type="entry name" value="MBT_PHF20L1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000205">
        <property type="entry name" value="2'-5'-oligoadenylate synthase-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000181">
        <property type="entry name" value="Ubiquitin family, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15204">
        <property type="entry name" value="LARGE PROLINE-RICH PROTEIN BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15204:SF0">
        <property type="entry name" value="LARGE PROLINE-RICH PROTEIN BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="447" mass="52830" checksum="F460EC1A12A4365F" modified="2022-10-12" version="1">METQITLKIKTLDNQITSLVVDPNNTVQQLKQVIQTQLNIPFDKQRLIYQGRVLENNKTLQDYKLQNDHVILLPGQPIQEDQLVQNQTQQQQQQQQTQGVNEFPEIDILSNILRTLRQHQYNRQNVRRMVQQQRSNGFNLDKQLSLEGIRQNYQTLKQLLDSQVKPEELKQEDLQGEYLNPFDPKKRKFQVGQWIDVKDTIDQWLEAQVQIVEQNRVFVHYNGWGNRWDEWIDTQSPRIATFRTYTVGSINQEFLSPNPVSDPDCEVEKQEIDMHKFIFDVGHMMNQVTQLLIEFGRFENNKRIAEKQIQLQQQYQQMLMNKNKEINTEQNIQEMMKKEKELNEYEIKSSLIGSQLAPIFDRMGRMMTDLAPHLAIMGSKTQRVNNNNQQQLLFQVPVTLTPNEMYQSQQNAPTLHSRLEIISNRLAVLRMTEYIQQEDSDDDHIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP75</accession>
    <name>A0A8S1JP75_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0060166</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JP75"/>
    <dbReference type="OMA" id="ITLQICR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="210"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="244"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8044084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="58091" checksum="1E366C0AAF6F83AD" modified="2022-10-12" version="1">MNSDILLTCRFSLHNNKQIEGFCPFPQCNNFRLFCVNCLLDYHQDHTNECKKLYEYPQWAEMFNQIHLENINKLESILEIFVILKQFKAWYETQYNPLIDNQESLNVQMFEQKITNMVNIKYITNIIDQSIKPQIEKMKTYLEYIMNQTGKIKNLFSYDEFIQSHIQSQSSLSNRSQRNYEETKSLYWAGKYQECINKCEAYQKFNITDVRFSLMKGDSYLELKNFEKAIENYQIAIEMDDSCYYAYGKIGDINLNTGNLQEALKHYDECTKINNQQQEYYIKKAKIYQKLNQFKQGLEQVDHLLQLNSTHIEALQMKGTQLNLNLATLLLQLDQKKSWIDQSQQSLQFSTPHIQQFLQHQLKLGNQILNGPLEGQILSKEEINFKYQQAYNQLEKNPKITLQICREIVEKEKCWLIERLVEMQMNRPMNRDMERDKINIQFLILSGHAYKNLGIYQEVLNICEIVKKIDPNNQEIESLEQIIYTLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQ05</accession>
    <name>A0A8S1JQ05_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050272</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQ05"/>
    <dbReference type="OMA" id="MFCCSTK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="120" mass="13875" checksum="66C93B08EE3ABBAB" modified="2022-10-12" version="1">MFCCSTKKKQKEQIVSTQKQNKILTEFSEEINDEAMTLYQSAYYQNISTVFQYPVEPNSMRSGSNSKTTRSVERLTNVLPIFVPRKTSFNNFKYSRTNQNTKFVSTKKGFTLLVTNTNPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTL2</accession>
    <name>A0A8S1JTL2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTL2"/>
    <dbReference type="OMA" id="MIFACAI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF83">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="494"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="630"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="685"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="709"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="786" mass="93827" checksum="F341008977E7B132" modified="2022-10-12" version="1">MITINNETNEEKILIQQQFSATPQQNEHRQEKEIQFQKVLDIVRQSDSNYFKYPPDLNLAQLHKQAYYVCPNQEVINKYQYNACPCCNKTINKKQFNWLTSDFYEQMIDEYGIVVPLYFTLMKFQVIIFVLVFCIYGIIFIKTVHKICDPQRGQEQQCDENFTYYCKMCEFSDNYHFIDIVNLQYYFSNQADNDEKNYFGFAAFFVFFINLHLPFLYDFLISYIEIKYWNREPCHRQTETKNSIYVRHLQHKMPPKEIFALIKRCVEKHPNQEIAIQSNKLIDPLFKEIIYIYDVEQLIKFASIREAVLIEIIQNLQNYQKYCLQKDFAQQNNCLTKLENNLTLLEDINNKIQKETGNIQFNQLTIDSLRNIDSLQFSKKAIIHFTDKQVFNLVHSNFKRTFLEDLKIKLKFIVHPKLNDKIAVKKSFRINGLFWNNLGTKSIDRVIQKCKSIVVMIFACAILMVAYEFIYLYVNDPEFQNNKETGRLTQGEKAATNIFTILVPIITTSAILAVIINQKKAKKNTFAHQERGFMHFLIVVNYLLVTFVPYLFTFELWNGKENPAAIYNLISLTQNKLITKHIFHMFHVRFISSLLKSRKALKNYLQYFQGQLNQILTPPFFPQRSRNCNALYSITIGLCLIYVCPLITLICFFFNIFMYIFDRYTITHIYAIDKRFTIILMRHQIKVYSIAFYPIKIYLFIKLFWDYEWLIYAGLPVCVSISLLNIIFRKQIINFILLTIFQMKKMDTNQYDQIIYSEDYINYLKNLRIDETNQITWQILNLYKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZU9</accession>
    <name>A0A8S1JZU9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0120277</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZU9"/>
    <dbReference type="OMA" id="YNAITIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001025">
        <property type="entry name" value="ATPase family, AAA domain-containing 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF12">
        <property type="entry name" value="PEROXISOMAL ATPASE PEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="663"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8048003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="919" mass="107925" checksum="E385A3E6E0D00821" modified="2022-10-12" version="1">MKTQQIVYFGCQGISDDNHIEIGFQFAQLLNIDDDDYIQINTVSNCNQYNAITIYVQKWSDYMIVQHQKDQFEKDLLNNIQIVYNELNFPIFYQEGQFVTFTFKDQDNNGPIAISPTCEINILIEESQKPEQKLDLIELIVLFSDQDYFESFIEINQELYNKNKLEKLYQITYSSSFPKSYRNFQKYAKNLKKNKQKQNIPKTAIVQFKYNTQINTKFCFISKFYAKQLGIREGQVILINTQEYDITKESTIRTIKRNQLIFQKIYFNFYTDLPLNKEFVKQLILEKIEKNEQTVLQSRVHLNKENLYFLRIVQLIGNQPEHFLQMLSIINEHNELKVIGEKEKGLLIQYLDEHVRILGFTSPIQKEIKFSQYQGIKKKEDKQQDYKLFQQLFDGSIKQIESILDQQSYSQCLVICKESQAIQVLVSYLKTKFEVIKLNLDKLNQQGQNEKLQNVKKFIKHIFWQSRQVSLNDILIVIKGVESIKSLDLIDFQQSFQILISNILSKLIRNQIMEYPLVKLIVVSPSKDYLNVNLQKKFQKDSIIEFNKPKLEHRQRIFEQCYQSQEIKKSAELLAQRSENFNFSQFHALQNREKYQEELNQQISEEWILQQINDLQDQQVQLNKKIPTFKEIGGLKEQKQIILDLFDLPMKYEHLFAKSKIKLPKSVLIYGMPGCGKTYFSLSICNELKINVLTVKGPELLDKYIGSSEQNVRDLFQKAQSLSPCIIFLDEIDSVVPVRTSSHSGVTDRVVNQFLCYLDGVEEGMKGVYIVAASSRPDLIDPAILRPGRIDKHVRIDLPNKDEILQILEIYQGPLEFDGISEQEIADQLFGLSQADVVSFFKEARIYLTDRIVQLEDFSLKRKVQKIEDFKLTKELFKDIMKSMKPSLSQKQILFYKDVYNRFEMGGIKDMSNQKQALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZY2</accession>
    <name>A0A8S1JZY2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZY2"/>
    <dbReference type="OMA" id="IHERELM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044715">
        <property type="entry name" value="WDR86-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489:SF11">
        <property type="entry name" value="WD REPEAT DOMAIN 86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="22"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="125"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="291"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="364" mass="42029" checksum="B1008FDC9F5E2527" modified="2022-10-12" version="1">MYQQQFAKPPKQIKSKAFKDRLKGHKDAIITLISPYGNNGNYLYSASRDGNVKSWDLLNRQIASKFLISKFSDDTTVQDQDENQQTMKGNIICAATFSEFNIYVGYDDGFIKSNNIKTGEPVYTYQGHKAQITSINLLNIRQLASSSLDGTVRLWDTTSNECEVIFNIGYPINSMNVIFDKEINLLYGKNTIVQIDPYKQSIVRSVQFPNFTIMSYLIHKTLLILGTIDNFIHIYELQQLDQPNPQPIKVIVGLHGWALCMLVHDPYFYVGTDDKKIKVYEMKNWELKEEFSGHIDGVTTLALANGLLYSGSYDHLIRSWDLEEMYQRIRERAIMEKEDLNSRRYDVYLKSLPKKKKALPKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1A6</accession>
    <name>A0A8S1K1A6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0120352</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1A6"/>
    <dbReference type="OMA" id="TNTRICK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12446">
        <property type="entry name" value="RRM_RBM25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052768">
        <property type="entry name" value="RBM25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034268">
        <property type="entry name" value="RBM25_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18806">
        <property type="entry name" value="RBM25 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18806:SF4">
        <property type="entry name" value="RNA-BINDING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="36"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8048172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="335" mass="40707" checksum="D020AAC374EE9EB7" modified="2022-10-12" version="1">MLPFFPVPGLQDLDIPQGVQQDFYPKSQIQEPQEAKKLFIKNLPQELTNDNVEKLLKECGQLVSWKRSKGTPFGYAEFENMESVLKCLRLLNGMILNQKELLVKGGEKAQMMIDGWMYKKETEWEERRAKLEEKDKQKEEYRALFKFTSFQEYITRDDDKIKKKLEKIISELEGTVTKTKNDKKREEEKKEHPRERERDAKKHNTRAELERKYKEKMAEWKKKEDERERERKKEKEREIDREKNFQKYLEKELAYDSEQERKQIGKIKMNERKKFRQREFEEDEIFKKKELLKTQKPPEPEQPQMVVIIQEPPEPEPQQTQQQLLQQQVIIQYYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1T8</accession>
    <name>A0A8S1K1T8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1T8"/>
    <dbReference type="OMA" id="ITNTMQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="33936" checksum="BDB85574D2B46207" modified="2022-10-12" version="1">MQQRDQVEDKKPLMDGLLDMFVRKSAITNTMQQVISITEPIINRGPSIEEQQTQIQENQFKYILVLMIHFGVALLMIGLLTHFNFKEQIQQIPFIIWVVLLMLYILSIALAQFSETSTQKQPKNVFLFGVNTIVRLFWFVYAVVLYPQLNLEVVIGLILINLIIILLCFHLDKSEGIKNCFLVSDQPWRISFVLLFEILFVGLYLNIQDAPSAIIWYNNNIIYIRILVLFVLYSFILMNIQLVEFRRHLAKNGNIYFLSQMYFDGDIVFPCYFLQHHLLCTSERSILRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7Z9</accession>
    <name>A0A8S1K7Z9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0170085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7Z9"/>
    <dbReference type="OMA" id="VHVQQFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="306" mass="35768" checksum="F26976BCDEC58A38" modified="2022-10-12" version="1">MNQQNSIPKIETKLIGDTLRFLKTTAQRNNYLKTLVKQKEKQKIIDEKQLFQQKQQVLQHSKKKFLSLLKSRNTELSFLKFRPIQKPLNLLRMCAELKISDRISKSVEIDEVIESKRSSKSNRTKHSSSQIFDHSVHVQQFKDESTPKQRGTLQFLSSQQLIKTTIPPQIKAEKKSNPSSVAQSPRQKITVRKLPLNELESKLEHLKSNSQDPNLQTGILCRVPSQILIRNGILLPTQKHQMKIVKCKECWEDDNSSLASHTELKLDSIYNNSKHLKQDHKNYEHKKVEYQQESIKLFYKKIYCKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K937</accession>
    <name>A0A8S1K937_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K937"/>
    <dbReference type="OMA" id="EWSIIPL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809:SF2">
        <property type="entry name" value="GH21273P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809">
        <property type="entry name" value="STROMAL CELL-DERIVED FACTOR 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIR" evidence="1">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="1">
        <location>
            <begin position="135"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="1">
        <location>
            <begin position="206"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="1">
        <location>
            <begin position="270"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="1">
        <location>
            <begin position="327"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="388" mass="44743" checksum="ABA05537E4F4D919" modified="2022-10-12" version="1">MYKQYDLQLFSGAIIQLKHLSTNDYLFSNRIKLTSGSKETMLGCTKNQYQEGTKFEIHLAYTDFGNMLYQPIKYGDLVYLKQQDQIVTININNKSQTTNQKEAILMEKQNEIMGSQIFVISPSELWQGSILKIKESIINQASIIRLINITSNLSIHSNSNYFKQKELSDCVEVIGCNERDQNDDWFVNIHDINLINGIIHLLPRFSKPIFHGSLVIIRNAFLGTALNSHSILLKSKNQEVTTNSIIPRISNELWEVVLMKIEGERKHSIENQLLYGEKFSLLHSNTNQFLKYNKGQTNSDKTFQVCCSDQNLFLEEWSIIPLSQQQTQAVFCNDFFLIKNNTCQQYLTSIAEPSYITKQQYKTFLTQLPTNASVWTIEAVLNNKQNYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCH9</accession>
    <name>A0A8S1KCH9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0190014</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="INNICIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035854871" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="27"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="995"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1063"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1094"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1167"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1195"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1226"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="57"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="91"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="137"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="180"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="226"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="277"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="321"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="366"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="417"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="506"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="544"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="574"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="635"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1245"/>
            <end position="1277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8052101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1295" mass="148782" checksum="387200854EF50634" modified="2022-10-12" version="1">MRYNSILVLLIFKTIIFPSNFQNILAQCCQTNCQQCVISICLQCNPGYYLSGSLCYSCIQSCIVCSSSTVCTQCNDNYWLNGNSCSPCTKPCANCLTNGNICNTCVDTINQTPPQCNCKDGYIMNTLTYLCDQCQHPCATCQITINHCLTCIDNINQTVDALNQCKCNSGFLMNGVNCEACLNPCLQCLGSANYCTECKDVEHYISNGQCICNSGYINDNNYNCIPCQYPCSTCSINDNHCDSCTDPNHQINAFFQCICKETYYSNTINTCAICIQPCAQCDINGCLTCIDSNQIINSSQNCICKQGYFQVGFNCQQCVMPCQTCELNQDHCLTCIDINQTQINNLCICNDGYFEINNNCQICQIPCTKCKIQNDHCLECLDPNHELISNKCVCKYGFGTSGVNNINCSLCQYPCLDCSSSTNTCLSCIDNNRFKLENNKCVCKQGYFDIGTDCLKCSSQCSTCSEQSSKCLSCSDPNHVLQLNDCYCKPGYYTDSYKNCLQCQLPCLTCNQDFCTSCQDEYYLVEGQCICKEGYYYVDFHCEQCNQNCSKCNSQFECTECIDQYYLQNTQCLKCQIPCLQCVDIQTCKTCSDNYIIDDQGKCIQCIQNCEYCIDSINCVKCFDEFYYHDQACIPCSNQCQTCEKNSNFCTSCKDSKQILNKKTGICYQINNYGDQYDYDNLEQILLLQDIRCNYNQLLIGYECINQCGNGILNEQYEQCDDGNQLGGDGCSFLCIQEDSYLCKHYVNSPSICSFIQPPDFRLNLLSNQQNQIKLIELSFTQKVKIPTDLNFEEIAIISIIPQTRININILNITSLSTEFEYPKYQIIVEFLDSVEDAYLQIEFAQSLIKNEFDLDLITYQMQLKLGNPFILSQQNQQKLTQIVLLNDAIMYSMISISGLAFLTGNAIMFFNLIDLLQSLSYIRYMQYDFPPHLIQFLNTYSKISLKPLLDHLKIDELFKYLNGGTLPFQNSKKSNQTQLNPLNQFYLLNAKGCYLSILVSLLTYLICTLLTSQIILQIQIKLFKKNKQNEKYLRFILFFQKKIQNFCAKFKKEYISFGLPKVYFAILHQLFFSALLQFPNYSFNSLFMTFNSINAIFCLILILLISLQLLSITQIKIKDRLKWKYFYQDSQPQFWAFNQKSFQIYRIMAYIFMIVEALNYPEIQSILLSMSSFFYLLYLIKFRPLKSKYEEAKLISKEFFFILISGSFLIYGFQLSFNQQLYYGWIHIALFTLMLISNLIIDLIECINKTLQKHKRKQEKLQKKEINNKMNQLQQFIILDTNQQYNSQGGKLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KG25</accession>
    <name>A0A8S1KG25_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0200201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KG25"/>
    <dbReference type="OMA" id="QTPRVQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="82"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="212" mass="24728" checksum="A48B82AFEB2A9620" modified="2022-10-12" version="1">MDLQHSKSYAALNQQNCQLFKPKPLSIRQQMAQLPTQTPRVQIQALTTPTTIECDSNPYLNQQLCSFKSYRISTEIINGSPLDQIQLILRENEKLKRSLNQKQQIIESLSRTRKVKPRLDFCDLKKNSRSLLQPSSQQESIKPKNIEVKQSISQNYNKRTKLPKIEDSIPTNEDDCNFTFANNFFNNNTCKNQKINFKQVFTQSHLKKKFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHI6</accession>
    <name>A0A8S1KHI6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0220321</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHI6"/>
    <dbReference type="OMA" id="QECEDTN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="11"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8054980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="529" mass="61222" checksum="BF62DF4C60990D21" modified="2022-10-12" version="1">MKHDFLKEKELKIIEKIGEGAFGQVFKGLYKGEEVAIKSMQGAQLQETSIMESLKHKNIIKFYKYLKQGNNQYLVMEYAGGGSLSDYMKQSLDEETISCIMKSIFGGIEYLHSKQIIHRDIKPDNILIKYSDDLSSVKIADFGLSYQYMPEIRYYQTVSQQCGTFIFMAPEQILNKSYNKAVDMWSCGVVLFMLLNQGKHPFYPRISTKKEFINSFPDLKYEQPLHASPLARDLLQRLLQYDQDSRYTAAQALVHPWITRNFNNPIPMSVKEFGTCQELKKKFFQFFKAVLFLNQVQQDSIKQSPIQSKTDLGDFKISIVEFDEDNYDQECEDTNSPKNQQFFNKLSIQRPSKILKSYRTLNNIPYKGKHYQLNAILNQTNESPIRELKKIKLQQQFSIDNEQFIIPIDPESPKKNLRGSQSNKLLLPALTESTQISLSPIKQKQKPLIKQNSFRKSDTSFFMGLNRMNNDSALEPGTLRNRVETYDKTQIPVINTQRFYGRNTITNLVISPKNNIATSTNLLIPKKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHR6</accession>
    <name>A0A8S1KHR6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0220178</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHR6"/>
    <dbReference type="OMA" id="WDINIHR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000015">
        <property type="entry name" value="Oxysterol binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF148">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="824"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8054729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="844" mass="99587" checksum="F099C50AB8CDF7B7" modified="2022-10-12" version="1">MYLKNLEFYSGSGYKQSGFLKKWKEKLLVFNLNTKVLQIQDAKNQTQKQIDISKYNLKKLGKYQEKWSIKLSTTSNEVKLGWNSEKEFQQWIDCFTGEWQKKQEHLRFLEEFDKSHPEIEPYIEKQPLVFRPKQSLMIRDKIENMIPHLPQFIQKDMEILLQNTLQNLDGFSDIQNFKQILDQENAKLYQDPNDNLHMKLVYYSKIEPRRIYDEHVMPSTQNWLPDVIKQFQVFPTKKGCSLVYLEFEFKEDYVIPRQHSIQEEELQEEQNNFKFHSSQSLNQNEQEENNKIRIVFTQKGFEKDGIYCVFRKYTGQEMYHDQIDRNLANQLGKYKIIRSALVVQPSNKQEFKSLLIEEIYVDCKDLDAGEKLLKILLLNITSSDRNLMQIQQEIQQITENVEVKNVELFETIQISIKLTESDIQRITSEQHQIQSKEEFQKLIEKVGNGQYILDAISQYSDQTIVSGLDKQLQTNDEEGHFLYTKYYRVDNNKGGLTYHNERLLKDQRSVLLNMIKRMGSNLIQGKSVMSVSLPIQIFESRSFLERMARAQGHAPIFLELAAKSNDPLQQLKQTIAFHMASFMMGIQQEKPFNPILGETFQGRILGCPIYLEQTSHHPPISNYIMFGRDYKLYGSFSPIVNMGYNSLSGEQHGHSIVHFQNTNLKFYYINQPFTVYNIITGQRNVNCHKRSFCFQPDLQLCAVIQFNPKDENKSFFSRQTTPIDHFLGKIYKVSPMCIQRCKMAHKTGSGLNLKLKLSKEEILETYDSISGRWTSHLDINGQRYWDINIHRPYVLELESRPLPSDCLYRLDLLLMKMKDIQRAQETKEKMEVDQRKDQKLRQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJB1</accession>
    <name>A0A8S1KJB1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090386</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0230247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJB1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="24996" checksum="FC0B8A98151DA368" modified="2022-10-12" version="1">MLRKLINTEFEQEYQDTKIKGIVISSSNFRKIMNVVQKIEKIQQDYQAAIVLEAQYRNYSVNIYCHWKIKKMLQIQQLKTFRHLIKQEINYQINNPYFDEVFKDFLNNFSFQQYREYFQQELIKLQFKFGLKLYDLQLTNNIEYPEFLIMNISQEFIRNVTQSDDSFKIKMKIILSTLLDQCQDIENEKLNNRKNIIRLLIRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJG8</accession>
    <name>A0A8S1KJG8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJG8"/>
    <dbReference type="OMA" id="CNNVPSY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="336" mass="39728" checksum="7472602CEDE877B6" modified="2022-10-12" version="1">MQLNQSPIPQEKLSFLNTLLDKELDMLKCNNVPSYLQKTQPSSYFEQESIQNSSIRALSNNPTSQTKRTTNGDLNEIQDKIVTIEARIQKNIEKQIQPNQLVQQQEFKDSDEETPLKNKYNHIQSSHKKQIKFTEQEQSTKTLNKSSFHEERKNKFQQLEKNIENAENKLSQSKAQRKSMSQVKSKSKEKSQTSLQRGTSTQQQQDVQSLQIKLSQLRKQWEDDRAQLIKEKQKNQQMQQQLDQLNKKLKKALQQNEKYSQIEQEQQRLQENFEKSEFIRQQQKQLIQTLKQEIEDFKSNNNNNNNNNNNNNNNNNNKSKQKKRGLEDNSNKYLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJV5</accession>
    <name>A0A8S1KJV5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Translation initiation factor beta propellor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0220116</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJV5"/>
    <dbReference type="OMA" id="LWGGPQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011400">
        <property type="entry name" value="EIF3B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 EIF3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8054611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="662" mass="78374" checksum="082544F2BF5D551E" modified="2022-10-12" version="1">MSQLQDQINFNDVDVAPEYIKRACDEQPKDILQEFPKLNFNPNLHILLIGLPKIKEEMKPKLITALKGFLKKNNFIQFQEIDLNNYGYAVLSYENEDQAQQAVEQISNQNFDAKHQLLAFTQKQINHINQTSEYIPITLKSRQQLLAFHKDARSALTIIQEKTKLSLKWLNSLEKKLEDNITEMKLPTYDKLQWSPLGQYLVLFTENEFSLYGAQDLELLHKFEHKKVNELLFSPDEQYVISYDGLQQFIIWNIREERQLRQIVQQQSSINHFKFKRDSKLLAFQTQDEIQIYEAPEFYQKNQLNLKAVGVKQIEWVPQTNYLAVVCYGKDIKTQIFLHDYNKHIDIKWRNIAFEIDSIQIFVHTYGKWVATLIKKKQKNKVFATTLQVGNLSKMDPFEIDEHTVNEDVENIFAELNTGKFALSYREKDKQQKPTSQFTFHLYSVQEDIKKGIKLCQLGEQKGKDTNELLWASNGNYFAMVNKTKNSPDQGKVEFGQISKKNEQPIIEITKSTNHFYMSHAQWDPTGTYFITMSDLWHSVNIWSAIGEQLVKDTITKVTDIHWRNKPIHLLSFKEEQELQNNHKQFIKKYEAEDDKILNKAKYDKEQKKKEMYNQFTQYLNEKRKIYNDTKEIRKKLLGWDEEDQNIYVQYKKIIKEEVVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KR96</accession>
    <name>A0A8S1KR96_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0260220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KR96"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014560">
        <property type="entry name" value="UCP030333_Alba"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947">
        <property type="entry name" value="DNA/RNA-BINDING PROTEIN ALBA 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947:SF36">
        <property type="entry name" value="DNA_RNA-BINDING PROTEIN ALBA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="2">
        <location>
            <begin position="21"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8057788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="113" mass="12879" checksum="4308894A152D2817" modified="2022-10-12" version="1">MNTATQQQQRGQKQKLDPTDNKLHVSSKKNAREFIFISKIFLKKFQNVELHALGEATKISVRVAENLQRQGLVTITKINSFTADIEGRKRVKLVVSLQLTQDGKARIDQEFQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRY1</accession>
    <name>A0A8S1KRY1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0250295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRY1"/>
    <dbReference type="OMA" id="YNAMQIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="70"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8057161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="360" mass="43028" checksum="303ED9FBD0CC0584" modified="2022-10-12" version="1">MSLQKIYYVPEPLKPSPNYREIVKKLIDSRQEDKQKIDEYLDHFQPTIDETREKQDQFLGKRYRSILQALERAKNNLVKYNLSLLELQDKIEKSVEIRKVLEDVNNYKTYSEALTENINKLQNQISEHTTRCKNLKRVIEYKHAALNKQNSKNLVLLREINAARHQLKKQKKTSDTQRTQQQTTFYPQKPLSQSSQKQEQLTDPQIDQIKYENKQMRYQLTSYRNNKYLKMELFNECMEAYKRYFSKSQKVVKNSGLQHSLLFFIQKAQFSLQDNAANNRYNHKNLKSVMAGRQIRNLVHDTLKQMAEDKQNKKNPSFDNLQLEWEIYKDYNAMQIVALMCLKPKIFAELSQMFQQEVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KW29</accession>
    <name>A0A8S1KW29_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0280195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KW29"/>
    <dbReference type="OMA" id="KEYKYEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.280.10:FF:000013">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826">
        <property type="entry name" value="COMPLEMENT COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT 1 Q SUBCOMPONENT-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8059027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="234" mass="27579" checksum="1E5A6ACBC163B575" modified="2022-10-12" version="1">MLRFMSFYRFSNISNARGALFKTVSKEYKYEYHQYEQEPTLMKQIKHLGFQLIDDPKTNKVTLKKEFPSAKISIEFRGTPPPLEGKIKVPKEDQSISQNPKYQDQIQSKIQERKEVYELGTLSVDYIVLVESEKGEAIAFECNTSNQTTFINYVQPVYDIQEYKTRSKYQKFLTEYPGPEINKLDEQLKQSFYTYLESFGINQSLNALIEAYSIDKEQRSYMEFLKSMEKFLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXE4</accession>
    <name>A0A8S1KXE4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0230274</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXE4"/>
    <dbReference type="OMA" id="QYFQTID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="306"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8055624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="526" mass="62836" checksum="5C78A2A645623B15" modified="2022-10-12" version="1">MNTKSTSLYIGSKQLNQKRIRSPLKKQSMIFKQNSDEEDNQSEDEKMEVINLNYFKAKKHQKKFNLQQMLQQGKLAEDEVDHNPHFPSPTINSKLIHNIFQIYHSDRKETFIEDKIHGTAEKISKQTNQKLVNQILFNKMRQKRVVSQHELNNSTSNVLQSFKNLSSIFKSVNQIVDYHAVANQIRQSQAQITINKEEHKNLERQQFNFNYIIEGRLNQIKLHSSEIYQYQMSLQKLRMENCKLRDHVSKLGEEIDQIKSKYNKQRQVALHQEDKLHPNKKWDRHQYFQTIDQNEQMELSRIKTFYMEYQQQLQFNQTQLKREEDMLQDIKYTKKQIKQDLAEFLKQVLQSEEIYVTQADFPCYLDNQSRIFLISKAKLEINNDNLKLQMKENQIKQQQQQGNRVNYEKKLFQVMNSISNMRKQKYTLKSFELQQQIDSKQDNVSQKLSNQSLQINNLDNQILELEEQEIQRLQILYQGKQLPNSSLQKLIKTVQAEFGYKQMNQKLSIIIQNYDTIKSQVNKSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYL4</accession>
    <name>A0A8S1KYL4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0300221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYL4"/>
    <dbReference type="OMA" id="RVEDHNS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035718197" evidence="2">
        <location>
            <begin position="21"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8060609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="357" mass="40745" checksum="743C9AFE89AF1E5A" modified="2022-10-12" version="1">MKTLFLIALIAIAFTARVQERSLSKITTDLKKSTYGSALLHLVELHSMAGGPVQELIDAIEELINDLEEELEELEFNFQQRTNQHNSLVIGFEQDIQDAVIDVNNTQDTLDNLLFPRREQLQVRIEQIQENQEANRKNYDEAVLTREQEHEDFEFQIAELNDATAAVDDALALLSSLTNPSLLQIKRFQNSLKNIEQKIKSRSRMAPMIKALITLASNQNFSDQGIIGQIVNALNEFRNAIVDSINAQTAAEADAQTEHEEYLEQLDAEYAEFQRQINRVNVDLTATNEKIDQLSDFRDQREADRKQYTAELELENNTYAEETDTYTNLKNEFTRELGISEQALSVVQSADFSNIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZF8</accession>
    <name>A0A8S1KZF8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZF8"/>
    <dbReference type="OMA" id="HYLLKCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40535">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40535:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035874268" evidence="1">
        <location>
            <begin position="24"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="26427" checksum="D3F134D1260471D1" modified="2022-10-12" version="1">MQILHLFLIVAVLSCDIDEAAKAFKSKQIRDPIFPYTKNPYDIVDPNYLQKVSDNLQDTTSVCAIKYDDYEKQIYHLKHFNSKEEAEQNQFIVTHQGKCGACSTLQDLAVYLTNDLTRPVRKCGLMYGLSQHHLLKCIKGLGFTDTCAQVWLYNTLNTKKSCFWPCIVSFMTNEDFVKNGKLNKCLQCDEDISGPIFKYESGRTRRNSGIKSEIDRPDDQIYDITHCYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1I4</accession>
    <name>A0A8S1L1I4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0320076</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LIMR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1I4"/>
    <dbReference type="OMA" id="KLIRSCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051584">
        <property type="entry name" value="GPCR-associated_LMBR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355:SF0">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8061678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="537" mass="63060" checksum="151D6979B98354CD" modified="2022-10-12" version="1">MIEGLLLLIYVVILVHEYSQQQVPFYVKLLTYISWILSFGIVFIIPHDIYYTLNDNGNGYEYTVIIWKWIYWGNFVLSWLILPICQEYEDAGEFTFKEKLIRSCKNNLIAYAYLIVLGLVFIGYLAIFNKLDFDSILKLLIALAYGFGILLVVILLGHGLVAIPREYWRKSQYEKCLRGLYLEAAQIHHAIQELYDQLINLTIEVIQHKNTNPDLTLHLLKNGQIPYEIIEEAQYKYKEKNRKLEPWPEVNKRVKKKASEYKRAITKWEHICTECFLLEDMIDNEFSVHYKIRSTLRYERSGIMGHYFDMLQWLWYTKIKKAYLLSLSVIFWILSSIVILSEISCFTQFDINILKRIINVNGFFQIQISILIPLMYISFCAFYGLFHINFAGMYGFYNHQQTDAPSLLFGSINFSRVSFPLTFNFLQMIQISGTPFEDVIGNMDTSSVLGMSFSYSLPILLILASVFNFFEVYDKILQGIGLPQFKFSQVQFSSKEGERLICRARVKRERDVLNKVGIKFFDQCEMRELDCQMIQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2F5</accession>
    <name>A0A8S1L2F5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0310185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2F5"/>
    <dbReference type="OMA" id="QITTSQC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="115"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="407" mass="47955" checksum="598D098FB97F80AD" modified="2022-10-12" version="1">MNNYQITTSQCSPNNILAVCVVIRKHFFKDRQYYLSLTSHSLTLSDGPNQKSPKYTQLLGLDIVFTWLLDQQNIIGFTLIYRGKVKEFYGKASDMQYLREKMGQLVIYDNIKKFYILNEKNEKGSFGKVMLGICKLSQKQVAIKQLEIQKNSQPVIKNEINILRTIYNYYSQNILELKEVFKDKQSYFIVTEFIEGYNLQQLLSTRKTPYSLQESLYIVEQVLKGLTQIHQSRVIHRDLKPSNIMYNYNKIKIIDFGLSCFCGKQLDEFPNCGTTGYCAPEVLNAQKTIQSYNYKVDIFSLGCIFYEILTLKKVFPYNKEQSTYYQNQNCLINIEEQGQIYDLIKLFLKEDPKQRLDTFQALKLVQNFIEMKNFDVNTWYKIQFEKDKQRNNSDENFIQNLSGIFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4A1</accession>
    <name>A0A8S1L4A1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0290059</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4A1"/>
    <dbReference type="OMA" id="ASQICHA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="46"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8059576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="335" mass="39981" checksum="E3AB570D0DBA427A" modified="2022-10-12" version="1">MRKNYIVIDDRERSRSKEKHVKQIQVIDLEESCYSLEKGDIIGGKYKYLERINEGTFGRVIKVRDVQSNSQRALKIIKNSFHNYEKEYQAVQFLNLFPKLIDHPGRHHIVKVYDFFYHKQCQCILMEALGPSLYESLVKKRFTQQQIKSIIQQLLNAIDYMHELQYLHTDIKLENILFKSQFDEVNVKIIDFGNVVFGRQFYRGLVNTEEYRAPEVILGLEWNSKQDIWCLGCLMHEMFFQKELFGKLKNNNQKHLLMIEYLNGPFPQWMIQDSKFFSKGRVISDQLMRQGISFSHLSDINIHSSFIELLRWMLKTDPKERPSAKELLRHNFFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L613</accession>
    <name>A0A8S1L613_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein kinase PLK</fullName>
            <ecNumber evidence="8">2.7.11.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Polo-like kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0340042</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.21" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8062889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L613"/>
    <dbReference type="OMA" id="VATDQVW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13118">
        <property type="entry name" value="POLO_box_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13117">
        <property type="entry name" value="POLO_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14099">
        <property type="entry name" value="STKc_PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001669">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.30:FF:000013">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.30:FF:000016">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000091">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033701">
        <property type="entry name" value="POLO_box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033695">
        <property type="entry name" value="POLO_box_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00659">
        <property type="entry name" value="POLO_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50078">
        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="32"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="11">
        <location>
            <begin position="436"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="11">
        <location>
            <begin position="536"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="334"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="337"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="387"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8062889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="633" mass="72053" checksum="7D7B92F2C1DAD0BD" modified="2022-10-12" version="1">MDEKTNQEGVEQLVIFEEKITTVNGQAAIKKYLRGKFLGKGGFAKCYEATNLETKRVLAAKIIVKSSLTKSRTRQKLISEIKIHKSLQNTNIVQFEHVFEDHENVYILLELCSNQTLNDLLKRRKRLTEIEVQCYVGQIVNALKYLHVQKVIHRDLKLGNLFLNKSMELKLGDFGLATKLEFEGEKKRTICGTPNYIAPEVLDGKVGHSFEVDVWSLGVIMYAMLIGKPPFETPDVKTTYKKIRQNLYSFPEHVLISDSAKSLISRILNLDPTQRPTLNEIMAHPFMNTGGTIPKTLPLSTLACPPSASYNKQFQPSTSSSNLKMSTNALPQRFIETTPNNPKNTQTNGSSDRFPLQKPSQSGNNMDDNFGSNCLNNAQNVGYGGTQRPQSQQPNEFRNSQSQKTFSQPFGGTGMQGAQSVNNLEVKQQQAKQEIHVKKWVDYSSRQGLGYLLSNGVTGVFFNDSTKIVLDTKTLQFEYMERKGQDKQDICETYNLNDYPQHLNQKVTFLQHFRSYLEGEFQTVQSEENEKKQLVYVKKWMKTRHAIMFRLSNKIVQVNFTDKTEIILSSEYKMVTYVNKTGERSHYPLATALDSQNKEMSKRLKYTKEILTHMLNGGDSKTNQLTNANLQNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9H5</accession>
    <name>A0A8S1L9H5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0290018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9H5"/>
    <dbReference type="OMA" id="INSKQAC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="1">
        <location>
            <begin position="24"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="934" mass="109873" checksum="66DC1D998EC8D27B" modified="2022-10-12" version="1">MLHNIYERILFAYTSIDQNLRQMAEKDLNQLEEQDSNFCCYLLELIQTHKQNNQVKFFTIIHLKNVIKRKWKAYKQMLGCEKRQGYFEENQRNQIKQQVLQIYCQEDVGQLKQEVLQILFELCSSDYPFKYPLLINFINQQLESNILEEQQLSFLKQIFKIMPKNQMKNDQYDRLWQKIAQLWVQADNLNTYSQQSLVLLDKMFTNFILSYNQIVFKDEISQIFTTILQKIARLLQSPQQQQYQKNINHLISKCSKFVDIYSFYLTQTFPDFLQLLSVTISSDKKFIIKGGLVAFIRFLKLSYLFQTDEQFQFYLKKKSDSEKQQFMKEKQIIDQILKQFFEKESQNFIQKFLTFITSSNDISIEEMIEQEEENEFEKILHPKDAEIFSLGLIAAELLIIRAPNICINLLQGYIDQTIQAQFQINANILESLLIIIGLIPKIQSKLKMQFQIQYPPIIQYLQSSNQIHHQRRLSILLNRLILILDQNQILSTLTLAGNLLSTSQDSIIQYQSLLCIKQVAIKYDGNLQWSQILVTVCPYIVKLLKCLQRTSIITPLLDLLSKLIEKCQNEQQEVVINAIENSGLLQLMQSKFQEAEENLLIIDQVCQMLKTLIVAYPLGTKISHIVQMASVLIINNINSKQACILELILLLLREYEEQSQNFPISQLINILQQNLDNLLSDTSPINSCIILQIIEELYLLGIFSINDFSKHIPYMESKYTLRDKNVKNSVLGLTTTMILMMLNNNRQDIAIFSGLIQQILNDLIEPEMNDRLDNKFRIEVQIVSILNRFIILARDDYFSFVQQNNISFEKYFQSWMKKSEILESEKSIKLNIIAFLLIIDYVQPNIFTAYITQIATQYGRFSKKQLIQKAITILSPCQRLSQYRASSRKEKIRNTQMHNEHNLQQYFQKIIQDYILKHNLQQDLTIIQALQIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9U9</accession>
    <name>A0A8S1L9U9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enkurin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0290135</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9U9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027012">
        <property type="entry name" value="Enkurin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052102">
        <property type="entry name" value="Enkurin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21490:SF0">
        <property type="entry name" value="ENKURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21490">
        <property type="entry name" value="ENKURIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13864">
        <property type="entry name" value="Enkurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51665">
        <property type="entry name" value="ENKURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enkurin" evidence="8">
        <location>
            <begin position="78"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8059724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="175" mass="20595" checksum="7FD564E4A15F38DC" modified="2022-10-12" version="1">MGSNQLPEVKQYKYPPSVRPPVPKKDEKPIHGLKSNKNFIVTNAVENILSAPKQIVDEKPWTEKKDYGKVPDYLTKIQQSISSEYEIIRNMHISEAEEMDKQKYLMTQEEVEQLKKGLKKKWESVNKEYQSITHIRMIDTVGLKRKKEQCEKELAQLEKDIEKLNKNYVFVDTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9Y0</accession>
    <name>A0A8S1L9Y0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Non-specific serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0340160</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9Y0"/>
    <dbReference type="OMA" id="NSARTIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14079">
        <property type="entry name" value="STKc_AMPK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000956">
        <property type="entry name" value="CAMK family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="14"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="8">
        <location>
            <begin position="480"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8063121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="61777" checksum="E42A4E0744372614" modified="2022-10-12" version="1">MQSQGQAPLQIEHYIIGKTLGVGAFGKVKLAKHNITNTQVAIKIINKRKMKNSRMGAKIRREIRLLRYFNHPNVIKLYEVLDTPGDIFVVMEYAERGELFDLIAQRGKLPESEARNFFLQILSGVEYCHNNLVAHRDLKPENILITHNYVVKIADFGLSNLMKDGKYLKTSCGSPNYAAPEVISGKTYCGSDADVWSCGVILYALLAGFLPFDEETTQALFKKIKSADYTIPSSFSPQVRDLINRMLTPDPLKRIKFHEIHLHPYMRSTQVPFYLQIPFKLDEGRRQINEDVFEKLMQLQTVNFRGMTQTQIQKSIRKREDKSFVVIYDLLLGQLGVESSTPMTLHNLTMHDLIFNPQIPQIEGQSFNNCLLNEIQKPQPYDYGKELPKDIMAIVYPFQARQIVNAIYTCLEKFNTVIKIKSPDYKLKCYHKNLIKMTKYNSSMELFNEFQKEVEETGSKNDLASLTFKDDGKKPKVKENKYSAKEIIFNIQIYKMPTNNNDHMIDFQLCRGHPVVFMDFCNKVIALLNQHFNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LC78</accession>
    <name>A0A8S1LC78_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0350032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LC78"/>
    <dbReference type="OMA" id="KKYFHTE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="159" mass="19068" checksum="EE8DE2C9A8B812AE" modified="2022-10-12" version="1">MNIKKPEKQYYLSPFPKDVIGLIASSCVQKFQGAYTLELQQKQQRSLQEELIYRYFKGRAPPYELSQADKDELQNFEGNEYHLCEYRGQQFLKKYFHTEVIKFNECIEICDIENASNYKGLLILKRSMCRRKCEDKMDITLKEYMRDGKFPKMEEILKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHR8</accession>
    <name>A0A8S1LHR8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0410109</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHR8"/>
    <dbReference type="OMA" id="STICWTG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049680">
        <property type="entry name" value="FLVCR1-2_SLC49-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 49 MEMBER A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="385"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8067678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="511" mass="58064" checksum="1F935FCDC0C7D527" modified="2022-10-12" version="1">MQKTYNQVKIEDCNQIDKETKSSTLLGSKIYSQYNYRWIILTCYLLVVFINGISYQTFIPNAKQFVQLYDVDEQIITLTGTIYLIMQPLFTFFASSIIVKKGFVVSTNLGVILTIVGYGIRLLINKYSFIFAILGQAFLGISRPFILNGQTTMAQNWFFPSNRTAVLAACNAFQSFSMVISVLWPANWIFKDYSYNKQNQQEGLDLSIQLQYQQFFFSLTLLPVFFLLKNNPKTPPSGFANSDHDVGFYQSIIKLLTNKNFILILFTFSLFFGTKKGFGLILPYIMSPFGFVDTDYSIASTMLIIGGFFSAGFVSKLVLKFKKYKTIGITLFIISLALTLLMYPILMTERLILLCIQQLLLGFFIIPMVPVLIEYSCESIYPLNGSFSVGVMVSGVTIAAMLSSILLTFTSKGQDSDKNSIFNTYIIFCCIQLTGFILFLFTKEILNRSKEVQQRAQSKQNSIVNPNGVYNEQIVGDYPTEHPILDSHQFIQNPEVLNPDFIEQMQNDEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKS1</accession>
    <name>A0A8S1LKS1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0400295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LKS1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="55" mass="6697" checksum="6CBF8638EC5DEC59" modified="2022-10-12" version="1">MKIFLYRVVLIRLKNFWLNRMNGIINKQLQIILVGPINQVEMNKKIKLYFVNMMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LU99</accession>
    <name>A0A8S1LU99_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0430249</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LU99"/>
    <dbReference type="OMA" id="QINVQEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="529"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8069203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="756" mass="89987" checksum="935EF9C1B01B1111" modified="2022-10-12" version="1">MYAFRKHAALNKKKLNAESFSFVMPKLNVNSVNEDPTASLLEQDKLPISKNQFANESILNQLEQKVGVQREQPQINVQEWGIQLSNHLKLIDDKSSNWVRNKYTKNAIEIINKQKFSTIENEKSQLSNQWILRFFSKLSVIEPTSNWLNLWSIFMSICLLIYFYFLMINLYFSKNEGDQEWYWDQWKQGYTGWLNMIYTLCMTLDILIEMHVSYFERGEYVKNKKQVYINYLKTGFFFDFIPLFVLYLCLISEQVNESTIIRYFFFFKFYKLSIYDQRFQERVQLHRKSKTIYLIFKIIVEILIIVQLNAALFYRAGIYMYDMKQEDEENFNWGTWLENDMFNGDIYSKSLQSQYFLSLYWSVATLTTVSYGDITPVNPLEILISTMAMLIAIFLFALNVQTIQEVYLEYKASKKKFQKHLIAINRFMRDKQISVSLQAKIRKYLDHIWDKQRAREQKLEQVVIDSLAPSLRQELMFESYGMDLIKIPWMSDNFTFEFIKELSEELREVYFAADETIFLDKETVHDDYSFYIVKEGQVDLFINSSNVAQPLVAHLKSGAVFGQYSFITGDGRQCSAKTRTQTTLYQVNRTTFINTLLKFEADYERYCQLKDLVMLGKRYDLLQLSCFTCNLSDHMSNCCPLTHFTFESQKNIMGSQIVKNEYKQQDRKKAKRRKAKQKNIIVNADEFEIDEAQLSELKQQYVEKPNDSYDIDKVHSFKYYFPCQNIDSFYYNQMRNISLNHKKYFTPPLRHQIVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUD1</accession>
    <name>A0A8S1LUD1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0430269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUD1"/>
    <dbReference type="OMA" id="SAKSQDN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="312"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="434" mass="51524" checksum="78546543C8699CC9" modified="2022-10-12" version="1">MQSLFVKCQKCQQRPATIKCNQCRYGQTFRLCYSCDSQIHNRSGPIDQQHKTEIIPYQEMYQKNQSIVPVPQKIDQKNSFKKNEMKPTGQVPTKDYLSNDSKKQDYSKTLDVNRVNNKHDYLEKKIDSNDKRVDQQYLSGDKRPYQSNQKFNLDQDRGSQSIINQLKEEQQQAERLRVELSQSRDKEKEFQRKLQKVEKDYEQKQQEDREKIQQLTEENRNLNNKLSQANKHLLDEVNKVKKQQQDQIKKLEQALKEKTEELENIAQDYNLEEIQSMIEQLQQESSMKDQIIEQLQQQLQDNQETVGELQEKLASSTKQKQQSSNKKSAKSQDNEKDEVIQELGQQLEAKDEEIHKLEDLIENFKQLYQHMSDEKQQLQEEVEKLANENNQFREIFSQNLHLFGIDPEQLNEEGEEGENEYPEEIAEENDEQND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LVJ5</accession>
    <name>A0A8S1LVJ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0500009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LVJ5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="189" mass="21896" checksum="7ABC2A21048AE617" modified="2022-10-12" version="1">MGSLSKKSIIWKLLLKSIIFNSISYIFKLSACSDATIPQTATTDTYNSLFRYSMKYYQRQLYKLHQFSYTGIPLRDGVAITLVNFWTVQEGKTKKYFVKLEQISILILQHTRIFQVHIPSAVTQLSGKKKFFQSITQDGKPCTYDEINLLLLINVILQIHAIHIMDQQVKKLLNMVIDLTKQIIHSEMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LVK7</accession>
    <name>A0A8S1LVK7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4200 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0430024</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8068836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LVK7"/>
    <dbReference type="OMA" id="HYKTKFA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051147">
        <property type="entry name" value="CFAP_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025252">
        <property type="entry name" value="DUF4200"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683:SF3">
        <property type="entry name" value="CILIA AND FLAGELLA ASSOCIATED PROTEIN 100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 42 LIKE-2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13863">
        <property type="entry name" value="DUF4200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4200" evidence="4">
        <location>
            <begin position="127"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="465"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="201"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="315"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="465"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8068836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="557" mass="66977" checksum="758CA52FFC4DDB68" modified="2022-10-12" version="1">MIDNPFQFEKDPKKFTNSLEYDRNAIEEEDDQKQQTRLTQLYIGKSASRYGLLRKLEEQTEKLAVERDLVFSQDLKSKSFNAKPDPIQCHLKQFNPRMLEKNYQDSLQKAYKIRSILSEKKETLQDFVEKKREICLTNLNILTKKEETNRLEDFIKNEQESLRARKLYFKNDCELVKRFMSEVKSQADYAAILADKEVRRKEEVKVEVARILAQIDRLKLQKSKLQDEYDKLDKYRQFLEKIKQHYKTKFSDLEKEIILPQTTTNSKRTLFLTGVNVMESETRSKEQLEQLQFEYKQNKMADTVIEILDAIEEGNLRNMQNQRDAEEEIEQKKREAQHLRETLAIHQKENEQRFKVLEKQYIMHQQYQQELKKQDKVDQNKLEFAEGENLDMDKIGKRIIEIYTDISKKEQDPLIKKLGIDSKMLKFTLNQLEKIVLELSEYKLQFLMKSKQDFIDAEKKINLKKKEQRQTNQKDEEKKKQIMERRRKKEEQMANFYRGRKDMRRNYPVEKVVVEQVVEDNDENDDEKYLQESYELVYVPSSKVDNIQSSQQLQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXM7</accession>
    <name>A0A8S1LXM7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0490032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXM7"/>
    <dbReference type="OMA" id="RNAQIHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000323">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052408">
        <property type="entry name" value="Exonuclease_MUT-7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47765">
        <property type="entry name" value="3'-5' EXONUCLEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47765:SF2">
        <property type="entry name" value="EXONUCLEASE MUT-7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="1">
        <location>
            <begin position="258"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="437" mass="51621" checksum="A0BF30254C0A68B1" modified="2022-10-12" version="1">MIGLSRFVYSIYQRLPKTLISYQITHSNKVYEMIMEKKNIREFDIFFDKRIKILNNIIHSNVFNDKLIEETKLKELILQNKTSEYIEELIKKKKYFTAYRFMNALQYDTVSYQELVYSMATSDMKLQSKIIKEQHLDTRDNQKVLNHLYGESMRFFIFRAEIPIEKVEELFLGDQNKLQFLVENYFTSNKQIAIQIAKRNNIKVQNHQIQQEIDKCIDVSQNALLKNDDFLPSEIILNTKKPNQLVLLRDFNISKEDVYMIEDEESLNDKIIDEILKAPQTGIDTESFQEIPQTKFSSRINKVCLLQIALPDKIFLLNSSNLTSSIKYQQFLIKYATSDALKIGQNIRQDFLSLLGQIGVYDVELKQMIELSQLFKMKFPEEKKTNLSFQCSKLLGKELDKVEQISNWQKRPLRNAQIHYAALDAFICLHLYNQYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4M5</accession>
    <name>A0A8S1M4M5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4M5"/>
    <dbReference type="OMA" id="VEGDYHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="19926" checksum="FA9BA3A0D5A37C42" modified="2022-10-12" version="1">MEEVQRYGQMELNMKEIISQVKSMAKASQILLMVLVIKVTGTHNLGEFYDNEIQGFGNYQWPDGRLYVGEWMRNKMHGKGEIKWPDGRKYKGEYENDKKHGKGVFIWEDGRKYIGIWQGGKQHGVGIYYSSDNVMRIGEWVEGKRVKWYDKEEINELERKGIIDDLRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5Q3</accession>
    <name>A0A8S1M5Q3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0500213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5Q3"/>
    <dbReference type="OMA" id="RIQLMEG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="335" mass="40045" checksum="6E487E99CE709244" modified="2022-10-12" version="1">MEELRKSIIKQEEEKMNNKLTLLEQSVGYPLNEYMNQEFINKIPEDSFLWQVLSNKYLVMKGEKPSKQDIREHKLYEEITFHDFVNECLQAIMFFHLIIMDFKLNLRIITDSIQTLQQQIQILKEYQQIKFDQPNRLSISYTRHKSLKTEIFRYYKIKYKVAMNQQEQKSIYSYGDDLTCSFPILPESNTLHFTILGKRIDGGDKFEILSKIDMGLDEVLVSFDLKTYSKEQIKPCILTLDYRDESQDAQYDGPNPIELRFQLQMPCEQRIQLMEGLLERQKKVEDQYREQMQLKNNSIQQIIEPFDDIIYIQGEDLKHRNSTEDREFCSACIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5Y2</accession>
    <name>A0A8S1M5Y2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0480171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5Y2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="251" mass="30527" checksum="282243183B3ACBAB" modified="2022-10-12" version="1">MSKQQSYSICDCGECENTIYQDNHNHDHNNHCDHNHNQDFYDHHDNFKFQLEQSQDTQFIKFYLEVQIINKQLQQHFLISHQLFKLFIQDQQVQVYIQQKRALIVYQIQQQLYLQSIVELKQKHFNQQKLFPLLCKDLNIYSHSHYVSILTMLQISRNAHEILEDLHVINHPTDKHEEKQHNELYYAQFRIALSFTGIFLFIEYIQQNLVTDKELLQQFIQNYKNLINLNLSKYYYTHNLNNACHYNVIFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M666</accession>
    <name>A0A8S1M666_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0520188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="PYIIQNF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1362"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1421"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1469"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1506"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1557"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1633"/>
            <end position="1652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1696"/>
            <end position="1718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1818" mass="212016" checksum="7161D57C4D31F84A" modified="2022-10-12" version="1">MAYQQFQLIIPYQGFCYWTDPKQENIIKSIFKQKIFSMQFVELVFQGDHSPSFFYQKISVINFDSLRIQDTLFLVQQQLQIYQNNDDNKLQDLIITNSVFFNDLNLPVKLQIISKNCQNINFTNLQFNNLNFLDSNIFEITPYGIQSIFTINKFTIENCIFTNTILFKIKNTSKIIISNLILNNCYFTNSSIANFEFDYNDPSNIQFHYLMIKNSVFIQSSIISNVVSLALMNISILLSKFEFTQIIEFKKDLNIKNISIKGNNFIQSQLIFKSPVKQLESKVIINSLIFEDNFHYNSSIFQTDLTLSNDLLSISFINFYLNQNQKIQLENQSNYLFNINSHNFSIYNLSISNSDNFNYFNLITITNIKVEYAIFVNLQTLHKVPILLECVNSFNINNQLFYIQGFNSLQFISITIKNFKSIDRSFIQIFSNLLTKEQEFINIQDLRFIENTLLKINLRNLFSLISIYSEKSQLIIINNIQFQENVYHQYIDDSSQTTASLLFIISQQSSVIITNLSCSQNILTNSTSSFIYINSKIINISEFQIQKHNILNMTTLYSLFEIKNIINQNQINTILLKVLKIQNTGGVLSFTTEDCTIINGILQYILTLNSKLFNIHTQGKGIIKIKKLEINLIESLDLNIAENEGCITINSQSSLLQLELTNILFTDILNKFASPILFIIPSQNINNIVIKNITIFNCFSLTNLLIKTQFSLMSIKNNKVLIQNVKIIQNKLEFKQYLQKFNTLSLIDRQKILNDNAIINLSGCIIIIKQFRYQGVLSSSILKLIDTYKVQMVKIYFIQIQLILKVDLIHIWHSPNNKYTVMIQDLTFQNITQLDQIEFLNLSYDYNKIEILNQNCSLINEIQIQSLKINDIGFDTFFQQLIQDSNQQGSLIYFQSQSILNKIFLSSIQAMNVNSQQLFNAILLFDLISFSSIDIIEFNCLFNSVNLYGCILAQQSDKLNSIIRITNSFFYKNNGGSGTGIKTKNVKLALYNSKLLNNYAQIQGGGLNLQLDQNEFVIINSQIQLNQANEAGGIYLLGNSNINENNFIKSVISLNKANLSPSNLLELPTHLSLSINQQDMLSIQKYIDNIQINILKLDPYQIIEQGKVIITNLLVLPSNQIIKNYQIFNPNDQSFQKYIQEFSISFKNNLNEKLIQFTNSTCKIQEQIFTYNKLEYTKLPQIIKFNTLFGNFDISQLQFSFDPYMEISQQQQLLIICQPNNSFSELQYGIKIATLKCQLGEFYIQNSCQTCQYKQGYYSVTYNSTKCSIFDKNKFYNITSNQINLKVGYWRPNYYSDDVEYCFKYPNICKGGWFVGDNLCFSGYIGGLCEECDKYNIRGEGYYYKNQYNLQCQLCEGLSNRILLFIVASLWSLGSIILTLSSIEKSNKLFISCKLRQRFSNIIFKLNQDHGSIIIKLFLNYLWIFSAIFSLNINFTFSFNFIDQTSNPSYFMANNLDCFLLQIHNIELIYLRIITMFILMISLLILIYLGFLFIELFYEKKFDASIISITALYLYVSNYAALIKQFFSLLAKRQISNINYIQGDVSLKFGTSDHIKWMISFIIPGLGLIGWLLPFLLLLLLYVKKDQIESIQFRKHFCYLFNEYNNSNFFWEWVKLAKKTSVIIILTYFETNIYLKATLLGLCLLFYQLYALEQQPYIIQNFNKIDVKAGQICSISIFLAVIKYICEQQDNFVLSFILQMILVLLSIKLSFPFINSLLKLYHKKYKRQFLIQLSKIFNIFQCTSCLNKYLKNKIYIWNQKEQRLRSNFYKLRHHLIYISKLQLEQQKSLTTLFTSESLPRHKQYTTELYLLKFRQDIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MA30</accession>
    <name>A0A8S1MA30_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0570209</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8077090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MA30"/>
    <dbReference type="OMA" id="ANNQEEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="242" mass="28522" checksum="DE388AE04602DF47" modified="2022-10-12" version="1">MKQQQIQEDSDSEYIQEVIIKPINERETKVKVTYKEINVRPGVLERQKWDKWGEVASLPRGQFREGDMLVEAELRIQAANNQEECNFIIPAPKQQFVVSFDKEQSKPQTNQQVGPRQIGQSIQFEQAPASSQWLVIVSNLVQFEYAETFQDIIRAKDDFRGWLRKQLEQEEGRFSYYINFLQNRRKQKVYSLDGQNSHQPLIQKVMLKFQDKNRAEIALEKLNGKSYDGCILQLELQEQRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAP1</accession>
    <name>A0A8S1MAP1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0580089</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAP1"/>
    <dbReference type="OMA" id="RNEESIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="245"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8077390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="441" mass="51572" checksum="131F95DE06B89CF3" modified="2022-10-12" version="1">MSKHKHRRTQSNSEVDLRNALCQQDDKKHFIEIFNHASHIQPQIPEKEVIIEKLEDDYAMQHLKENCGLTFGNSKKKSNFGSYQPNNLFLIPEQNDLLSSVEQTQESGKKLTSGIKLNVNTQKRQTYSVEQKNNNDQKENLKNEHNRQNSTLISQIQAQLRDVTHLMNSDKLDLFNKMSFTKELQKKTTVLPVQVPFSHQTTLQPSLNHNYSQQQQSQSQHQQASNQTQNNILQQQNLNIQSQQLVHQNSQKNVTKHTITPDRKRTPSMAINKPSGIEAPFQKSDKSINENEKLKNNLSVSIFNNLFQQNKKISISQIANKPDQQNDSFRKLETQMKKIERELMSLKQRQDQQDEINKTIQQQLQQVIHDSRKQREQGHLSLKKLEQLEMITRRNEESIMYLRQTLGGHKRIDSTTHQTDSTELSDQHRRFTDLKSLNRYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAS1</accession>
    <name>A0A8S1MAS1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0580109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAS1"/>
    <dbReference type="OMA" id="INQDEHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="160"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="327" mass="38668" checksum="F82C95E4E2DBF77C" modified="2022-10-12" version="1">MQLVEEQQEIDNDVDYQHVYNSSLFRKLTSQQIRKILNPYKQNTKEVLSELSNQIDNLEEGLKKYQNENTMIPPSKQQILDILQQNTAFTPTESIQSLLSEKTKLKTSYLHVDNLYINQDEHIPQTKLEIMQMALSNKAIQKQQRKAIKSDDLTKKRKIIMQIQQRINQDEIMRKKKEEEERLRLLEKQAREVDVGQLTLNMISDLRKKYFNELVSTDTEEKFKRIKEIKEVYVPQQLNKVKEGYQKQQQEKLKHDLVSKKSQFNSYYQLLALERTMKRVVALKLKPEIKSYSRFKGAEKPKNYSSINSIVKGIIHTSKNSENKGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBJ5</accession>
    <name>A0A8S1MBJ5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphoenolpyruvate carboxykinase (ATP)</fullName>
            <ecNumber evidence="3">4.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0540030</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>oxaloacetate + ATP = phosphoenolpyruvate + ADP + CO2</text>
            <dbReference type="Rhea" id="RHEA:18617"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.49" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBJ5"/>
    <dbReference type="OMA" id="NTWPDKN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004612">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00484">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.8.10:FF:000001">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00453">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001272">
        <property type="entry name" value="PEP_carboxykinase_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015994">
        <property type="entry name" value="PEPCK_ATP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00224">
        <property type="entry name" value="pckA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006820">
        <property type="entry name" value="PRK09344.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006821">
        <property type="entry name" value="PRK09344.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01293">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006294">
        <property type="entry name" value="PEP_crbxkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00532">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8075093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="543" mass="61371" checksum="E3494C3CAB622C41" modified="2022-10-12" version="1">MENQTVQAQELLTKWGLRNATVFRNPSVPLLYELSMSQPLSEDPTVRPDSINNTGALVAYSGKKCGRVPKDKRIIKDEVTERDVWWGDVNIALSQDSYNEVEKIALEYLNNREKLFIIDGYAGWDINYRLRIRVFCTRPYHALFMKNMLIRPTDEELKKDFSGDVDFYIFNAGPQTIQKPIEGIKSEGCVAVNLTERKMVILGTQYAGEMKKGVFGVTHYLFPKQGILTLHSSANEGENGDVTLLFGLSGTGKTTLSADPKRRLIGDDEHAWSDNGIFNIEGGCYAKCVDLSKEKEPEIYNAVRFGAVLENIEYYSEETREVDFTNISITENTRVSYPLEYIPGAKFPAQGDHPKNIFFLTCDAYGVLPPVSMLTPEQAMYHFISGYTAKVAGTEVGVKEPQATFSACFGEAFLALHPTLYANMLAEKIKKYDTKVWLINTGWSGGKYGVGQRMSLKYTRGIIDLIHTGELKDVEFENFPIFNFAIPKSAKNIPTQILNPRNTWKNPEEFDKQVLELAEKFQINFKKYEDKATQEVINAGPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCI1</accession>
    <name>A0A8S1MCI1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0540184</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCI1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="427" mass="50910" checksum="AA62C2377DA5E6E3" modified="2022-10-12" version="1">MKLKQFFIVAVKPIKINKTQERIGKLPSKYLLSPVLKEQNDINLQELKEQTNLDFQYQYQPNKIGRQNDYFLTQEQYREFAYKNPHGKVHIFKYQDVNFTLAGSQWEHVPIKNLYKLLLFSRPSLIMLQMKPDQILKGFNYQFKSSKDYINQIVRDPEEIMPVGYHEKIAEILQHDKLEFQEYEPYINKERIDYDKIATISYYAKKFNAKIMLADIPALIFRESLCNQLTLPQLQQYFQIACMATPQNPDIFPNTPMNVAYQIIPHIFLKRSDEFMTTLIEYLAVKKSYKRVFGIFGNMQSDAIVDLLDNKCASHLRYELEIPKIKKTLFKDLTCEDLVERHAILDVMYYGDIEEEIDPEQQDYYFPETMAIIDKYADPQFIDKTRSAQMRYLHLEMLKKYSIFQQKYYKIGKSKLKEEYMEKANLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MFV5</accession>
    <name>A0A8S1MFV5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cell differentiation protein rcd1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0560080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MFV5"/>
    <dbReference type="OMA" id="EKVYTWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000334">
        <property type="entry name" value="Cell differentiation protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007216">
        <property type="entry name" value="CNOT9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12262">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04078">
        <property type="entry name" value="Rcd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="39451" checksum="3FE8F95E81B8310B" modified="2022-10-12" version="1">MSNQYVIVPQNYRSDSMVSISPADKRYFEQAQAKQKSPPQQMMFGGYSPSPSLQKSLSTQLSEQSIDDFSRMQKDSDTELVVQYILQLRDADKREQALSELSKKRESFPHLAPLLWHSVGTIAIFLQEIAVVYQHLQPAQLTPAQSSRICSVLGLLQCLALHVQTRSCFLRAHIPLFLYPFLNTSNKSKAFENLRVTSLGVIGALVKGDDPEAINFLMQTEIIPLCLRIMKKGQELSRTVATFIVQKILLDDNGLNYICQTPERFFAVSQVLQTMIDDLHQSQKDDQRLLRHIIRCYLRLSENQKAGEVLKKYLPQVLKDPTQSFIKDEVVKKWHNNLLQNLSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MIH4</accession>
    <name>A0A8S1MIH4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0630004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8079948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MIH4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="138" mass="15984" checksum="311C3B3FCD979763" modified="2022-10-12" version="1">MGLQDSYCNLYPKAYYLISQKDQNDAQFSRSQDDVIFNKTTGDYNLDNLIVFFNPTYNQDIVLLLLIEFNSVSFPQSEESHPFLTYNYVTNYQLHADLKTFSFKLGGFFNQSLGGCVFYDTFNIILNVFGSSNLQLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJC1</accession>
    <name>A0A8S1MJC1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CWF21 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0600126</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJC1"/>
    <dbReference type="OMA" id="FKAHQVH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21372">
        <property type="entry name" value="cwf21_CWC21-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051372">
        <property type="entry name" value="CWC21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013170">
        <property type="entry name" value="mRNA_splic_Cwf21_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36562">
        <property type="entry name" value="SERINE/ARGININE REPETITIVE MATRIX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36562:SF5">
        <property type="entry name" value="SERINE_ARGININE REPETITIVE MATRIX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08312">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01115">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="CWF21" evidence="8">
        <location>
            <begin position="58"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="114"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="114"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="151"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8078561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="223" mass="26464" checksum="A2D8C8C7F3B251C2" modified="2022-10-12" version="1">MYNNIGLMTPRGSGTSGYVQKNLAHIKPTRRQDEFLKEIKAMKENVIQARKKANPEIILHEMKRDIELKKITLQEELEARGMAEEEIQQRVQRLEEKLKDMLNKGEYQLDHVADTHTKTQRKEEQEKKIGDAFGIDKEQFKPGTAFDFDAEEKSRLEKKVEREMRKAERLIQLKEQKKAEKKRLKELAQQQQQIKVAQENDVKKEESRSRSRRKEKKSKKHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLF4</accession>
    <name>A0A8S1MLF4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein eS31 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0610215</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLF4"/>
    <dbReference type="OMA" id="TANELMY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Small ribosomal subunit protein eS31" evidence="5">
        <location>
            <begin position="95"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="161"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8079251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="187" mass="20425" checksum="B591BACA39EFBC80" modified="2022-10-12" version="1">MIIQVGHLNGEVKTYNLADQCTANELMYILNSELACEVQLVQCGELVNASSILAGDRIYYVTVDAEGGKKKKKKKKNFAKPKKKKHRHRKIKLATLKLYNVDNKGVVQRSHKQCPQCPQGVYMAKHFDRHYCGTCHQTFRMDEATIKANLEAIKKQQAAKAAAAAAAAPAGGAGDKGAAGGKKGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQ46</accession>
    <name>A0A8S1MQ46_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0690151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQ46"/>
    <dbReference type="OMA" id="IILEPHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035750870" evidence="1">
        <location>
            <begin position="19"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="711" mass="82384" checksum="A1358D1ADC0415EC" modified="2022-10-12" version="1">MIVQLPLLIIMLLKLIESQKVLEFSSFTDDTFTNFDGWNLIVVQQQGSGIDKPPLLTQSYFGFNSVFWDRSNNIFDQYSGNSVFQGMIKYYNNLKPHKALIVTFSIYILNTQISPTGNCLINLFIDGSRKFQVKPTFSQFQKEIKQNLELTLSHTASSIFIQFASQAIDTDKVTFGIRDFYLYSIPCPNNCIHCKITDSQEYECIDWQLSLESLIEIDRNLFNSDGWTIIQASPSMKIHKSTDCSSENINSMQILGFMEMGDQVRKTIILEPHYRIKIQYMHIVLQQSFAPSTFWQGQLNGKTEYLIGYQTFEKSPTFCSRIIDRTRGDLISQVEFESFHQDTIFEFSSLPNQSFKGWKTMWGIRNFQVLIKKCHPNCQYSCTGPKQSQCQNIKYPKFTLIANNLKLITFSDDDDWQVVSAFQFSQPNECNNQALLGGYNNLQGKHLLQSIYDLKTHIKIGISFKIYYIDQFNNDMLFVDVDGKIVYQYAIPSTIDSRKDMPYCGVYAQIDQVMKISVVSIPHTRSQVIIQIYTNQPDASTAYWGIREFILTVNGFPYLKNLNDVKFSNNNISPWKIILKNSPIWQCGSKSFIGGSNALDKDSYLLKKFQNLPSHVQIRISFSILIIQPSLRKTQFILTQKINDFKKQDYILIQDRKFCGGTEYTHLFKFDYILDNQDEEIFLLVQIEDMNVGEFWGLRDFSLSYNDVKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MV76</accession>
    <name>A0A8S1MV76_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0650124</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MV76"/>
    <dbReference type="OMA" id="YDHEGHR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="525"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="527"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8081246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="740" mass="88963" checksum="8653B93E9AA4CE9E" modified="2022-10-12" version="1">MEPERSKEILSNLLAKQIDLNEQLQNENDELNTNLKRLLNLLNQKDSQLEQIKDRIIQVDQTENQYQTTISNQIQQIKQIQFDNQELQLQIQQQQEIRFKMEQELNKIIESFERMQKINQQQQEEINLLQERNQQLEETQITNQIQIEQTQKQIKLLVSSNEDYEQQTDILLKQNNELKQIQIKLLYNLDAIQKEKDELTTQFDEKLIQFKSCFQSSAENTKDLTNQVNELTNQIQILQKQNQQLIEQINNYQQLSVKLEQERDKYHKDLEITIQNNTRLQNSNSNLENQLTQISQDQETSIKKMKYMIEQIDELELTNDSQLKQNNELKQQIKKLQFQQDQLLKEKEVIYEQFELIQKNAQYDHEGHREKYIKLDRTYKEFHSQYDELLNKYNIQNQSYEEQQNQIQQNIKEINELKHNLRILQESERKHSYNADIISKEKFMITQKLEESFQIIKDLRDQCQILTEKLQKIEIFNKQLLLENEQLQKQIKVQEGKIEEHEVHIELLQVQINHKQTQQAELDRQQSLKQSTTKKSQSVNKENNSELIRLKKQNTELTKEAISLNEQILELKNVINKLNDQLAQQNKIKLGLESRIQSLIKVVEEFQQKQIQKQQDSLTKEQFQNQIEQIKKNFYIQIQQLTRENEELKKLLQTQANQTFNSTEEVNSLQDTVADLNREKVNLIRELNQEHEAKNKSEAERDKLKQQIEQYQLEIENCQNQLDLYVKVLKQMDEKLKQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MX94</accession>
    <name>A0A8S1MX94_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MX94"/>
    <dbReference type="OMA" id="MIYLICN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="163" mass="19437" checksum="9D4EA6AF04C293BB" modified="2022-10-12" version="1">MNQHQEDAEIFDIPTSKRNSELTGRALSSTIFAIIYNFGWGLLFLLFRHYNDQSCDSINAWSLYAEIFLFMMAGYKLIIELPIQYKTNGFWKEKLFDVVDYIELFFNIIILIGLTYAYLQHEECIHLRMFILSYLIVTYFIIFIWILTMIYLICNKDEQSRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXY7</accession>
    <name>A0A8S1MXY7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0750009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXY7"/>
    <dbReference type="OMA" id="TKKEWIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="308"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="62337" checksum="5FA99FADA1C19615" modified="2022-10-12" version="1">MENQQQKNQMNCTRHIQFPIISICIAPHQCQCQRKLCIDCQYEHGVDIKYTIPTHRFQDMFIKQLREFKIDQTSELTQKRKNFLMILSQTESMLKNTWQQLQESIQQIFETIEMQDEQYFKIMNDNFNPTDLSDTDLEQLVQIVQEKKLNEWKAQKDSCFQKLSKVQDLWETEIKAFNENLIKEMKEILPSDIKGKQQQNEMKSSSQFHLKSFTYQIIEANSIKQQEHCAAVAINKDCSIVATGCNKLIKIYEFQQGMLKLNQVLNEHQSEVSTLNFMKQSNQLISGGCGSILIWQYNNNSWICSQTIKGHSQYIRCIILNNNEDLFISSSDDKTIKFWVKTKKEWICQQTISDHYSQVYQLSLNEQQNQVISCGNDGFILVIEQLEPNKNWIVKQKIQVDCYGYRLCFINNNIFTFQPENGNLMYVYEMNNVNRQFTKSKDITLNQGNEGCWLFPQQFNKQKQLVVNKHDKYVHLIRFTENAEFKVEQSIQFGKNNIFGQLSDNGEYLITWDQASYEIQIRRFQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXZ0</accession>
    <name>A0A8S1MXZ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0730140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8085090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXZ0"/>
    <dbReference type="OMA" id="DIIQTQW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="259" mass="29081" checksum="0C63BA21E74C91C0" modified="2022-10-12" version="1">MSLQGLIKTGVLIGNVALLTGGYVLVKMLYNNYLNFKKEAKRLEKGGQSNYQYLVDAFNKEGKVGEVVQINNLSFIGTSEPIAPTTFVHSKINNIEKYICIKGVLHEYNRDIIQTQWIKAFNLKDENNHQVTILVNNKESFMPSLFKRVYEPKDDQTQGQLVKEAFSVKVTETGIAFGSNLYVFGSFIKNKLGQIQCVESKLICNASDAKSQTSIQSYIFWVGAACVVTCCLLISGITLYLTFSDSDEKKNKQQSAIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N046</accession>
    <name>A0A8S1N046_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NUP210 Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0750167</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ILIIMNY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055097">
        <property type="entry name" value="Ig_NUP210_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045197">
        <property type="entry name" value="NUP210-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23019:SF0">
        <property type="entry name" value="NUCLEAR PORE MEMBRANE GLYCOPROTEIN 210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23019">
        <property type="entry name" value="NUCLEAR PORE MEMBRANE GLYCOPROTEIN GP210-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22969">
        <property type="entry name" value="Ig_NUP210_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035914093" description="NUP210 Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1496"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="domain" description="NUP210 Ig-like" evidence="3">
        <location>
            <begin position="112"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8085980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1515" mass="174604" checksum="478E8F2EEAC7F88B" modified="2022-10-12" version="1">MKFALILALAILNTINSSKISNKNILLPLADIEVEYEITAEGGCFDWSASNSAIVVTGQDQYGCLKSIGKVRVVTKMPSQTFIYVKSKSQDVSQFIVEVVVQDISRLSIITKQKQLDLDTQEELHVQAYDDKGNTFTTLEGLKFEWRTGSLEMVKFSESGLKVSEKRARLEFNSDIIVVKGKKEGKETVFTRVIEKKYYETKIETNVDLVVIQKFQFSPDYPVYYLPTHSAIQFHLLRADGKTNIKLPSETHQWSTTSKISKINQNGVLTTQNVEMNLNLKVINSNYNQIQAIYHVVNPKYIDIDIWEDGKDKREGKVTHLIVGKLYRFQAYLKDESNQRIYSTDLFEYNSKDIILNEQTVISDIERQNVKLTFQRGTLESFITIHFVKPIQIQTIFKTYIHLPINEQYNLIVNGGSGNYKYLSRYQSPTIFEIKSPNILQALELGENILIIYDEFNIYNSLEIIVYVTDVSQILPFERRKELIVNETDDTFYQAIGDPKVGNYTQCRVVKFSIDNFDTFTTQQIIGDLSNYLVCNGLKMQSSTPGQYNLQINTQKISVTQQVRVYDYLHFEKPFYYVTPHSTITTKVLGGPTTWDQTPYNEKLSEGIIKIDMEKYHFNCFQNQAEFKITRINKKSDLLPNPKLVSNTLPVKCLLPEAFKIYNEKNQEVNWLFKEETIQINVVALYKTFLFYNSSSLHLDYSTRGLKYQNRYLSYDIQSGISDCKFIVASNSYNNHKDTFLTIKSELDLIIHHTLKLIPSGEQYVLIDQSVLFRIESSTNNIQCQYDNNIIGCEKDQVTITPKKLGQFQLVVYDLSLNYSVSATLNVIDPSQLNLELSQQITVIGNLINATSTFNYYKKQVHVTTWSSLRLIDSYGFNNINQVNNQFLITPNKEGIFKLQTQYDSINSNQVDLKVIPKLQAETVYMPTECETHVLFPSYSQFTYSAISNEDLETSVSQNIVTIKSKKIYGNYIVTIFLRQSNILIDSVNIPVVVNKPNRAILYYGRKIELGSSVRIVADFLIQDHQMNLCLCPTHKIHWYLDEKLVQTAPNSLGLSVSTVVVGKIIIKIKAYDLEAQTIIEVERLNSYTRNLFINTKALYHIPLLIPTNSRLALGQEIKSVQQLNMNSPNIIESDSITLQPQLLQATTPMLIQVEEIYQMYPMQEFVNLKVDEKLEIQIAYLNQEGYQFEDIEDNKLEVLGYTRHIVQIKVNKNGIEIQGLDQGFALIKLNCGNNKIDYILVKVGATLLQKKAYLGSTITYRMDNSEKPIWNSNCGSFKGNQLTIEKELNECYVEVNDQGNKFKAGLEVIRPSHIIIESNKVDNYLNIKLNVNPKIPQSDQVNPNFEIKIEVDQPQWFELVKTDNLYEYNIKPIIAQDNTPMPKKVKVNAFISNQFIQLDGSKEITYEREFYLPNKEIFIQNSQPIQILRIPYYRNIEKTYQDNHLLGLITTQFSQGRVEIVFDFSQCHEPTEGDVYFENGQKIRVKFVQESSYELIIFLAALVFFILIIMNYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0Z9</accession>
    <name>A0A8S1N0Z9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0640153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0Z9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="80" mass="9198" checksum="6A389FD9BA8D7286" modified="2022-10-12" version="1">MIWKKLDALLSKAETKGFKMANKAHVYVVNGILCLIAYNVYTVFRGYNEFFLEARQEAAPDLDDVTGEPQYPINKNIRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2A2</accession>
    <name>A0A8S1N2A2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H(+)-exporting diphosphatase</fullName>
            <ecNumber evidence="2">7.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0780154</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.3.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2A2"/>
    <dbReference type="OMA" id="FDSMLVI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009678">
        <property type="term" value="F:diphosphate hydrolysis-driven proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004131">
        <property type="entry name" value="PPase-energised_H-pump"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31998">
        <property type="entry name" value="K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03030">
        <property type="entry name" value="H_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="111"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="515"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="545"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="619"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="645"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="712"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8087347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="731" mass="81904" checksum="05EFF159CAEA47DB" modified="2022-10-12" version="1">MFTDLSVYLIVSIFCVLAIALFLFGFLGTNFSIKQEEDYQNLIIDYGNLYDEQEIDEQNWKQELPAYIATYLQTIEISLKELYLNQLIITIITSAGFLLIIASWLTPSGTFWGFFISFLWGTIITCVIAYLIIAVKIGTLLKIPIKASIGKEDILKWIYGNGFFTALFMGGIITLNSLFIVLIMKAMSVQSTKPENYAIMVSGFPLGMLISGIYFREGMSVTGRGIQAACDLMIKKEQYFGDIGNSLSYTTKIAAGMSSLMAEVNFFDSMLVLSLFVSMPSIVLVNSVDYHYSDNFIGKIFGIQLVFILSWIGQLLISFLSHILKNDDAVAFTANNVRCQILMSIFLSLCFVIFSLLSLPKEFEFGDKEHSQIYREKVLNRDFMGCGFIGIVVSAILIIYYEYLTSHGYSVAQKVALISYKAPTLNIIQAYSIGNIGQIVPALLIALSLFISYKLASLVGILGLFLGYIMNIYILMGISMLGILSGDGLKLSFLAQFANNIKDRLHSITWAAKNYIVWLKITNAGALIIAAILIIGTAIFFSKTFILLLNGYNLFGFLIGFGLAYYNKGITIFIVKTICEEALYDEKSFQLRKNKPDEEIALENFSYTYFPLKNRQGNYYVLVNLLSCTIAVLFTGIIFGHGASISLAISNLFINAIMMFQSILTGTVLKNAFIYNSYDQQQQETSQVLSVNLYGDIQGQTVEESQNIQNPYYMIYIMLITLVGIELFKHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N3Z7</accession>
    <name>A0A8S1N3Z7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0720057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N3Z7"/>
    <dbReference type="OMA" id="MFKNIQC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8084426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="106" mass="12594" checksum="45E551C07557F483" modified="2022-10-12" version="1">MMMKKRLLLQQLDLAAPKKQTGPYKQFLQEQLAIQHLKYPQRTTDELTKLINLMWKVREKKNNQMYFQHNGYVNISETERVPVPQPPPPVLIMFKNIQCEKASKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7C8</accession>
    <name>A0A8S1N7C8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histidine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0750120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7C8"/>
    <dbReference type="OMA" id="ESVYWMT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="120"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="394"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8085886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="521" mass="60116" checksum="9429AA0FD3E86C94" modified="2022-10-12" version="1">MKQLKQQLIKMLLDHIGAPEDVNNLDDNQISNRPFLSSIKIDDPIQAILNQKEEQFNLIYRYKEIGKNGITKIKVIKISLLQQFLKSEEITAVMIQNVSCKERKKELTQFIKIQNSILNSFSHELRTPLNSSLQLLEALSTKLSDQMNQEYIQPALNSNKLLLFQINDILDYAAMQSNQFVHKYSQFNIKEIADYIKQLYTQVCQNKGILLTCKIKLTNQIVTNDKQRIIQVLINLLNNSIKFSPSNTHIRVQFKNKFKYSNQYIKIKVKDQGFGISEQKLSFIFRELHSVSSEESVYWMTQNQLSSGIGLKMSNRLIQGLAFNEHRKNAFSIKSVLQKYTQISFYISDMIILKDDNESISNAQSDDALCISVHKSLDYKLIQNINIKSCNCSAILLVDDVPFNIQALKTILMQQKITTDSAYNGIEAIDLVINKYQQKKCHPFYQLIVMDIEMPLLNGLQATQKIISFFNSINVKPPVIVASSAYDSNKEDLSIFSDTLPKPIDQWRLKFILNKYLTNFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7G6</accession>
    <name>A0A8S1N7G6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0750150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7G6"/>
    <dbReference type="OMA" id="SEQQIVH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8085946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="282" mass="33141" checksum="F2928EC1FCC794A2" modified="2022-10-12" version="1">MNNDNQNAQVILKASLTRTRITKILQKKPDFQLKLKLDALDSIISNLPTEEESPPQQDLLPFQSSSILDKAKELITARKQKINFDSVYLELKKETCYEDSFNQLIVQNLDKYNNHQDVTLKKAPIIIKHKSEQQIVHGYQLLPKIAVSNQIKRKKETMKLLNQQLKSDQQLVKQQTLRELQQMKLPDIFQNVTINDLKQKENIQKFVLQGEFYQQWKKSFKLRQPPSLVQSFKSMNQYLLDSADYLDKVNKIDKKKIKDQMESLTEFVGVQYEKLKIKTDPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NA32</accession>
    <name>A0A8S1NA32_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0760184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NA32"/>
    <dbReference type="OMA" id="QHTNIKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="91"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8086423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="148" mass="17779" checksum="4EB179B691B0119A" modified="2022-10-12" version="1">MIQFQLASAIFLKEFLKIVDLQNFNIESIDERIEQLKIQILQNQQFNGFLKEEQNYNPQTKCEYPELENHIQQKTKKQHGNKFTSNGKQYFQCSKCTKIFNHPSSLSRHKKNQHKNIKHDLFRVQKLQTQQQQQQQQDIVINFTDLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAX0</accession>
    <name>A0A8S1NAX0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0780075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NAX0"/>
    <dbReference type="OMA" id="NTSHRNK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="164" mass="19626" checksum="7D52D28F923CA3D5" modified="2022-10-12" version="1">MNQVDNLLKLSLSTKKSQLLDNNLNQDFFNQWEQKLLQKETQLIQKEKQLKEKENQLSQKENLLVKKELDLNKRMMKYSTILEQIIEQKQTKSDTQLLPFNFNNTSHRNKNINEYQNRVINLLNTIRSNSQQQQQFINQQSYMGSTLELTEDRFNKSSRGFQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCJ6</accession>
    <name>A0A8S1NCJ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0780101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCJ6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="2">
        <location>
            <begin position="79"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8087243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="379" mass="44914" checksum="7B35E267A671C27A" modified="2022-10-12" version="1">MIKNRGSSSNKTTNPYALQMQEEAKKMADKLAKLKQNLNSQKMERSQISKQSGDSTETFRWQNNTTPNNRILKDRPPSNKSEPIEQFLKSIKLDTYVQKFKENGFEELDILLDIQKEQLQQMEIPLGHQIRLMKKIRELKQFVEVNEPIIQNESKYDELEEPIIDQSFTQQIKRSSLQKERQKSVKKVTFQQNENQEEYKSIQQSFNQSFILDQLQEISNYMNQNHIGENSNISVQTSADQNQFDNNTSAISITKQKPYDEQNAQLLRIKKVACWNCFKLLIKDFIYIDCNEFCNLECYQKYNNLYLGQCVSCSQQFDIRNGIMLNGCYFCTEKCSEKYDFQIMKDKNEEIEKQFLNYKGNHLQQEIHYQKEEIDLNFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDV0</accession>
    <name>A0A8S1NDV0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0800087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDV0"/>
    <dbReference type="OMA" id="FAFINDA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653:SF1">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="298" mass="35282" checksum="AEC9A555F9D2A7B3" modified="2022-10-12" version="1">MQLPTRIIDGLYLGDQGAAHDLEFLVTNKITKIINCAAKHIPNHWESIGIEYLSYQWLESETQFTFDHSRCFAFINDALEEGEAVLVHSIRALNRSVFVVVVFLMRKFKWTLQKTLQYIHNLKNDFEIKSNVFQQLLNYEKWLHVSKLSQNWDIAHNEDEILARNTYLNSQRTYSNESKRKKEIGLKKVQWKQNLVSQFIPPYYQIEFKKFSTPQPKMQYSYMPIKLRIQPSDNNWMNKKYTLNQIVDEQLQRKLNLFSIKSDRQSIKPLPRPGTAPSNRLLKGKVLTSSHRQLVKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NH31</accession>
    <name>A0A8S1NH31_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0890186</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8092038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="AYQIREY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431">
        <property type="entry name" value="FGFR1 ONCOGENE PARTNER/LISH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431:SF4">
        <property type="entry name" value="PROTEIN TONNEAU 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2023"/>
            <end position="2042"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2054"/>
            <end position="2077"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2127"/>
            <end position="2145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2227"/>
            <end position="2249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="931"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="937"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8092038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2325" mass="276706" checksum="951CE2FDC3529FEE" modified="2022-10-12" version="1">MKQKLTFSFVPSIPLDIQIQNLRKKILQDQNLKKICFKEFQNQYRIDHLLKVQSNLRQINTIQQVKFSSTVLSIEHFALLAQWLKQLQCNNFIIKLNNNKVDNNEIHDFLNAIVKGDIQIKKIIITNNPGIYKGQLISELKKLIRQQNANNYQYQYKLVLNKQYFSLAIIRQNYNETQQQRDFQEQKVFQKISKLNFFSFSFNSTFTQINFRALEQLINLNFNENGIDQISKLQISSSLIVNSAIMCEQMSTSKGLLKMVMQSQSLLNNSVPIENLMGNRYHKFRVNVLDIEKNSTILEKTYKILSDNIFLHCKQIKFSSSLPQQLSTLYKMFVMTESFQQYSNQLIQQSIEQGKILKQNLRVLDLTSLENYNRHDLIAKFLQALVFTETSNLKKLTIQNIDIGRTQAYIQALIRFREYIQQQQQKYPFYQNFKLPLKTLKLPNQTYRMEIATMKQFFQEFFFTRVGDILEIEEFIMEKCFRQSNREEGLKENCAEIFQLLQANPDIRYSIKRIKFIESTYQFDSVTYRCLFLNHCFNIEELMIKKDTIAGEFIQFVNETEQFFLNQPLDYKLPLQIITLDDIEDIVFNISDLLQFFVFHDRMKIKKLVLKQIVLLYSQLSINKLEEVINSKDKNQILSLEEFEFSNLTDDVGVLQFFKYVVFNKNIRIKKLKFGNLDFSACVYFLQQYFETQNELNVNHSLQELNYLKISNVNFEDEISWEFFFQKFVLHPTMKLKSLKIQQQKLDEAFYQGISQSFENSGEILKLELKELKYKKCEIMVDPLTAFLYTCSLNHLSFDSCIKMDQGLKICMDNYHQSNVKQYNNFKLESFSIKMSKVEDTKIFKWLIKEIALNIQHQQLKKLNLSDCNLSNEHLRILQEQIELIKDNNQKQKSAFKLYNLKIDQNQQISESTWIDFFNVLMKNKALVDQDDQNSEKTNINQQKHNNNQKKKLKQTKLINDKEQQLSINRIKIEDQQDREIKEKIDTLQKNIIETTLYRNDLNLKQKIINNYHIFTPNFPKQLKQLHLNMNFQELDQNDKTQIYERLISGLIMHPENQLTDLTLVNLNLILFMEKCEQFLIYYNVYQNQCIKNEDNKWSSKLESLSLKSLATQNQKSTDQFVKTFICSQSVNLQKLLLENFNEQLIVSLMNQIDNRQDYKITELTLLCIYESIESKYFNSFVDDIIYCQKFPLQKIELIGMGIQSNNKQISSQNKQLKQIQYKQKQISLKQITIQLIDEQQVKTFISILGHLLQQPDCKLEVIKIQTKAQQENLSSNFFSQLTNELKDIQELNLKEIHVQGKVIFDETFVQFLDKCILSLEQLSISNVDFPYSQKLKDKLNKLKRKEKKSKFTIEIKQIATGIEHFFECFTFNKNIKATNIILSNPQNFTSLRFENMSQFIKSIKLDMGLLYSSTVKLNRDSLTLLSQRFIYNEQSHVEEMLLYQCQLKREEIEAFCLPAAEFREQIIQQQRQGICQLKLKQFSIYYSLYIGEVGCEIISKDLIFFEYIDIERIDFLVTNFNDQMCDHFSQAAKNWMTFQQLNNRKNKDKYPLKYLDIGRNEFVQSKTTWANLLQTLVFTDRATELSTLNLQFMGFNDETISYLVEQAQLYLDQKPDNYVLPIKKINFSQNNALTHRGWQNLFDNFFLHKKVNLIELNMISTMLDTQIKLNTIYECIQKRALQEPNQKLQLQVFLCYNVSLKNAIEPYLQVPAKDYPKPKNLPVDIDYDSWKYGIFDGIPEEFGSIIMLVNTLIKKRNEFIETNKWKLFEDIRFPNYHLNFHQHYLAILNSYLYQQNNKLANLYLNLSTLDIMCQFFNYTTFIRATPYPYRLIFSEESLQYLSEQQIKLKKLKLIQIPFAKLEQLSQLDVLQIWQQIRQLNIQIQKIEMEYYLNDELIEEMFRQNYNESDLLDLITLIPPAKIRIENTLTLNTLKGFYSILQNQYYFSSSIIKYSFNDWINTGLAYQIREYAYNYVPKTSIQSKLKTIAYNISNWLVMPQQTYIYYNENQSLQQYLDKNANPLFIISLINMFYLILLIILPLQQIFIYSINHELNFSLVNSFQFIILISTIYELYLINNLKQENKPKTFKQILFRQVQDLPRLVSQKCIPYFNAISIIMYYSSDSNIIFTIFIIIYFCYYLQLLFQNTTIMYQYYKNKKVILQDIYHLSRIGKFSEIETIINEILPYNSTIIKKTKLTKNILPKSIYGKAIHNKIYFNLRDFILKDLPFLILNTIFIISQLLMNTLTLYQQLSQIGQLVQIYISFKNFISYRPSVVSQDDFDTICKRNQNNYKRKKLNLLQKEKKQLDLYANIYNEKELKNKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJW5</accession>
    <name>A0A8S1NJW5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0770169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJW5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8086934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="88" mass="10418" checksum="5E6C5DD5688E9AE7" modified="2022-10-12" version="1">MISLSQLKAKNFLVTTAIKYYKVLHIEKPKPKKKIRTQSPIQVPNRKRRLSFCLKENNQTQKNKFPLMPLHLHQKPNNSRIVNKLSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NMS2</accession>
    <name>A0A8S1NMS2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0890010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NMS2"/>
    <dbReference type="OMA" id="IGFQQTD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="327" mass="38400" checksum="D8EB0FC568D5436C" modified="2022-10-12" version="1">MSNFQNRVQKLQCEEVNHDNKPIILICFNKECQKRKVCLTCIEEFHQNHKGELASIEDVEAFIRKNCIKQRGAFQCNDILGKFDEFKNDILSQLKVLEFILKEKVRQLFDNLNNTENKLQQIGDECENDLNDLQNKCKQQIEEFKGEWNKKLNQFKSILTPIQINLKRFEKLSEKDIDLSAQQVNISVLYPKQTIKLTKLNLSLLEKTFHDKQIELEFSIFKNMNLQEKLHNWKTVIDHNKMIIQNDSYQIQLPDNVYFKEGECYSLEIKSNQDVKFKLSNEQSLENQLIGFQQTDYEKASFKSDEKITIISQSTSGLLVSVGALFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P273</accession>
    <name>A0A8S1P273_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1030165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P273"/>
    <dbReference type="OMA" id="EKNDIGQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="114"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8097305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="182" mass="21728" checksum="19A6E43CC084FC57" modified="2022-10-12" version="1">MDPSSSQTYIKQHPQDNSKSNENKDGSQTQIKSQTNFRNGQEKNDIGQFKDSELQTIEFIADNIKYQLDPRYMLWSTELLKQDKPQDQNAYNYQQENQSIKKKSHKFMDLKKLYEHNKSTIEGLNKELNDLELARQKTSQRLYEVKQEKERMRKIFKVERTKTENLKGCKERLDKLKKELGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P551</accession>
    <name>A0A8S1P551_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Single-stranded DNA binding protein Ssb-like OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1060012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P551"/>
    <dbReference type="OMA" id="PGINAHT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048970">
        <property type="entry name" value="OB_Ssb-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31472">
        <property type="entry name" value="OS05G0244600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31472:SF5">
        <property type="entry name" value="OS05G0244600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21473">
        <property type="entry name" value="OB_Ssb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Single-stranded DNA binding protein Ssb-like OB fold" evidence="2">
        <location>
            <begin position="24"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8098025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="191" mass="22350" checksum="AF6E820E94B352D0" modified="2022-10-12" version="1">MASQNRPPQTRQLTKPKFDKLSDVKPGIHGYNTFVKIEEIKKEQIKRYDGSLLVVSEGLAGDDTGVIKFRVVGEYANILEKGKCYAWRNGLSEVVQERHRLSLDQFGRITPEKDDLVVVNQNGKKYSDIEYVRKDARPNTNRRDNRDNRDNRDNRDNRDNRDNRDNRDNRDNRGQRRPQGNRPRGGNQKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6Z8</accession>
    <name>A0A8S1P6Z8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial</fullName>
            <ecNumber evidence="7">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Succinyl-CoA synthetase beta chain</fullName>
            <shortName evidence="7">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0940202</name>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1080017</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 8">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8094066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6Z8"/>
    <dbReference type="OMA" id="KQMIGNR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000001">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001913">
        <property type="entry name" value="PRK00696.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF10">
        <property type="entry name" value="SUCCINATE--COA LIGASE [GDP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp fold succinyl-CoA synthetase-type" evidence="10">
        <location>
            <begin position="20"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-citrate synthase/succinyl-CoA ligase C-terminal" evidence="9">
        <location>
            <begin position="291"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="72"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="350"/>
            <end position="352"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8094066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8098810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="425" mass="46079" checksum="91FED1C3D7207579" modified="2022-10-12" version="1">MFNRITRLAALQHKVVKCFDLHEYQSKDLMRGFNVRVQKGAIALNADEAAKVAKTLDPSGGLILKSQVHAGGRGKGTLSSGLKGGVKICKTPEEVANYTKQMIGYKLVTHQTPKEGLQVNAVLVHEGVDIVRQLYLAFILDRNSQKPAIVASINGGMEIEEVAKTDPNSIIVLPIDVNTGLTDQIANKVVDTLQLQSVRQQAIEQLRNLYKMFISLDATQVEINPWATDPKNQLFCIDAKINVDDNAKFRQKELVELRKTSVASEQVDPHEELALAAGLNYVALDGNIGCMVNGAGLAMATMDIIKLYGGEPANFLDVGGGANVEQVKTAFEILNSHPKVETILINIFGGIMKCNIIAEGIIKAAQLVDLKTPLVVRLTGTNSQQGAKMLDEFAKSQTKVSITTATDLDDAAQKSVKIAHTSKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7Z7</accession>
    <name>A0A8S1P7Z7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1090056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7Z7"/>
    <dbReference type="OMA" id="PCDEIEE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="69073" checksum="59F4187FCC1A73AA" modified="2022-10-12" version="1">MPKILESIYQGLLKLKNEFVYQLDIFGQLPSFTVLNRSKYTSKFGFVMSILIGSLSAYYLITEISSMISKSKPSIYQTEVQVIETDSYYLKHENFTIAISIANRFSEPLIGINKYFNLNVSQCTRERIRNQSTGNITVMLRCVEMPIEPCNMSHFTTDLQKEYFKTIRFGGVQCINRDYMKKNPPVLKGQFNALEHKYLYIQFTVCRNTSTFQGCAPKEEIEDILQAGHYNVYKTDYISQLDHPGEPFKQVITNEFTGFSYSTSKMILQQYRIVQTTTDEGLIWELENVQQNIQQTDWREISDFYNQQYLVLHIIRLDFKQTNNIRTYIKLQTILGKLGGILQIFIMVVAIISKPIIDNLMKLEMANSLYRFYGQSEKQIQLPTQAQIIQTEREFQSPINLNNQQTESLNTDSNQKLNKSMLETFLIIFGFKKEKHEQFLKARTKIIKNLDIVTILKKLQEIDLLKKILFSKEQMEILKNLPPPLITTKPSISLKLLQRDLQKEKDNHSNISPLQCQQIKCNQFQFVPKLNPILSKSYIDNQLQLYLDHCNDSQKHKVKHTSSLLKSPCSPISDIQLIKCDEIEEPTPQKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PA15</accession>
    <name>A0A8S1PA15_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aurora kinase</fullName>
            <ecNumber evidence="13">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T1110038</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="13"/>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8099942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PA15"/>
    <dbReference type="OMA" id="YTFFQNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="123"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 11">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="250"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="10">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8099942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="386" mass="44982" checksum="44A104D0DBF408C5" modified="2022-10-12" version="1">MNSLIQQHRSASSRHRSFLNITASQQLRVQSPLTYTENSQINQKDLIHKLVQTLRLQPTKKNKENKNRQQKARSQLNSGQKQRVLEDRLQKLETANSPTLGSQNRRIFNLTLPHITQPRYKSFKMLQFLGKGRYSNVHLAIDENTNYHVALKIMKKAQIQSMSQSIAQEIKIQYLLNHPNIVKLYTFFQNTDEIVLVLEYCPNGQIHKILKGLPECCFPEKLASSYIRQILSALIYLHRNGIIHRDLKPENIFLCYNQIKIADFTYSVYSPEDQRQTQCGSLAYLSPEIIRGENYDKSTDMWSLGVLAYELCCGETPWEDLRQDEMQQMIQDGIIKMPDTFSCELKDFISKLVTSDSKSRMSAKQAMTHPWVQQQEEQLTQYEFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCG3</accession>
    <name>A0A8S1PCG3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1140050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8100982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCG3"/>
    <dbReference type="OMA" id="TQTNFRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="41"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="164"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="358"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="437" mass="52856" checksum="FE7B515D2B0314D1" modified="2022-10-12" version="1">MQFKPAPSNLNQIRAQKVKFDDKELTFLKKEKKLEQRITELMQELQQIPKLQEQIKNLNEQIEKQESTIQRFRLVQSNERTLNERIQNKEITIQNQEHEIEQLNKKIDQLQKDVCYEKQNKQITYQIKQPVKSLTVQQEQEKPIRVQELEKQLLYKTSAVLKVQEKKRQMKKQYKNRIEQLEQELKDYKYGYETYYEQNEQLTEQLRDTSALCGQLTSENQSLKDQLELFKCVPSEERDKNQDTKYIIEIQNLRNRNLQQQQQISQLQYDISELKTQNGILIEKLKTIDSVKQKNDKLQGELMLLETENERLKRKNLSDLDHIEQQIVQISLKEQQIKDKNYELELKQINLHQMIKYTNGMKEENEQLNSEIVILKSQIIDLRRQFEFVEEKHEEEMRILEEKLKLQQTQTNFRIINQIPVQINSPYQIPQIERHYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCQ1</accession>
    <name>A0A8S1PCQ1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1120073</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCQ1"/>
    <dbReference type="OMA" id="WIYLAYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="360"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="622"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8100368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="749" mass="88040" checksum="B38E47E34E66BD97" modified="2022-10-12" version="1">MIKHKLIIFQIICNSTAAIVISAFFKEYISLSIYGSILLFTILFCILKQQKLQQVIQFILILLIQENSKYNKMEFQHFQFQLFWIYLAYQLIQNNSTFELPILLELLFSSIINIIISFEAYNSKFYYSITTASIYTLSIITYYYYKNKESFIKSLDLELVTPKINEFQRIFTNKTQTPRNNEIQIFDHLPIGLVVLTQDHDFQYMNKRARTLLERASNTQITEDNVVIIIKELLKKCINDKIASVGNLSRPSQTRIHQLLSKFNQKQRVSMPHLDDNFIKQIDPINSILHQYNDFVPYSQQFMDSKYRIQYTYKGKKRCFRFHFMKHHYQIIINLLDETSKIGKLDQKSKHIFQNQLLNSFSHELKTPLNCIQQLLEVIMLKVNSELQENIVKPIKWQTDLLLCQINDILDYASFEINDFNWDFSLFYLEDLLQECISIYFQGCKQKNIELKIESNSEDKHMINNDYKRLKQVIVNLLNNSIKFTQQYGEIVLKIQELKNNMYQIQVIDSGLGLSQEQLCQLQINIEQDNLENEYNQFHVGLGLKVANRLIIGLSSLKKGLKINSTLNKGTIISFIIEDFIEDNKSQASETQVKDVQLSRQLSQLTTREFKFIKQIICKCNKILIVDDIPFNHHALKMILNELNCQADSVYDGQQAIDLVQQRIKNNKCHPFYSLILMDIEMPKQNGFEASLKIKQILGDQSDQTTIVMCSAYDNQDCIKMCQRCLMSDVLPKPITKMSLQYILEKYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCT9</accession>
    <name>A0A8S1PCT9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1140121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8101122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCT9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000028">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF148">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="370" mass="42494" checksum="90BAACE4EE6F5FA7" modified="2022-10-12" version="1">MNQSWRQKFEKDNQYPRHPMAHKNGGVHMNQEDDTKLFQIAGAMIKQIGSKIAKGDFNLATITKPICLTAPKTANECMTFDHDYANLYLTEAAKCKDPLQRMIMVVTNEIAYLHGTHTYLRSLAPIDPMIGETCQKVKEDGTAFYCEFIKADPPTTLYQIYGDGWQIYGSEEVLAEIHPTFTQIIGKNLKPKYIKFADGKLYEIKVPGMIINGLMKGDRILNKLDSFTIKCVQDKLVAQINFSYVYEGSAQKIKNKLMFWSQQQKPLSDLVDVKINKLINTDDDDEDQVFTLSKGSGSWLSHFEMDGELLWRIDDPIQPWKEPTYILQSDSIFREDKELMLKGDDEAAQISRNKIEELGQKDQNLRKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PL79</accession>
    <name>A0A8S1PL79_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1220163</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8104045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PL79"/>
    <dbReference type="OMA" id="QEVATNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035751753" evidence="3">
        <location>
            <begin position="19"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="265"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8104045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="331" mass="38771" checksum="857C594FBFB8F496" modified="2022-10-12" version="1">MKIAYLLLCLVVIQGIEFESFQQAIDFDPASIGSPNCQTDDEYTNTYEAFFPWITVLEEGEIEMQEDHSVLSQAKEKIIQAREFIESLDEELQQEKQQESNNQQNESVPQVQDVQTEKVEEAEQVDEDDEEEDPFGWGSGEIPQLLVHKKERKAHKNTHAFIQIMNGNKLSKFKSKISKLISMATEMQDNQDENGQKRRQRKIELCDEIIDELTDMILFLEKVMNDYDYQNEIYELFKQKQQQIQEVATNCGQRVISYQDVSLIEEKKQQEKKKIQHQIKSKRRNPVSFLQIVIDDEESQQHQLEYQKQILQSQGMLGFLRASGKQVKLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLE3</accession>
    <name>A0A8S1PLE3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1220154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8104027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLE3"/>
    <dbReference type="OMA" id="AGKMTHG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="78" mass="9433" checksum="9516D7BFDCD4EBC4" modified="2022-10-12" version="1">MDTPPQNNYKHNVDDNYRHDHCQKFQTKTGKLTQNQQARQFQEKDQLLKMQNDLRGQLKYIDETNWMFEGLNNANDNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMT7</accession>
    <name>A0A8S1PMT7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1230052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMT7"/>
    <dbReference type="OMA" id="KIDYQYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="661"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="814" mass="95797" checksum="BEB5B2CEBFD8C24B" modified="2022-10-12" version="1">MSSNINHYKYTCASNQNTIIDQETVDISDIENYHALEIKSMDYLFVCKKSSKCLYLVSLIDSYEKYYLNTDKDITDFALAYFAHSYYLIIIQNKEEIQFFKIDYQYKFSLNNELKIEGLQQPKLYCSQQIGTILIDNTQIYLIQLDNKFIKQQLKQVVKLVIITKCLKYIFILTQDSKLQVLNSKSLQIINEHNLKYTESEPIKFQLFELNNQIALFTITSQKRIIFWSFDKEDLHTSSLLPSESVKLKFNSSKQNIELFDFQIDSTQNFLFILYHTQDMTVQIYIAQLNQSQIQKKEKSKIKHIKKFVKESSTSSLFIQFIIPPEQTTKFLHGGNKCLSEKEYIEFQQGFKDFQNQELEFTDHELPYIFGLQSEKKDKIELCWFYSDLVLGAILPENLLQLYDQNTSKKQGSSIIQQSHLIPPPYIKEEDVQQLQQQQKWEINSNNTNQNNNSLNQFDPIIKANEIIENELIPKVQQCQQQQQQFQMISNDVKKDLIEDITFIFDIKDLIEAQVKKEIDKQGQNDQQALHKLELQFKQDLCQLETQMENVVTQNLELYQQTCENFLMDNFNQTYFEQLIDDKISTHLKLLANQQITQNECTQLIQNSTNDYLKNQIGQLYQVELAKMSQGIVQIFDKVIQYFNDMQFIDQTKLANFNAFIDNATQQLQTLSKNITQLAENNRPGSDALQKLDQLIQQTEQMVKQYEVAKKGLKLTEHQYQLPQQQQNIQSDDRLQKIEEHILKLRNDLQSLTQNQQVQFQPVVGMDQFIKGIAAQLTQLQIKLTNPQMNIQLIQEILNQLILQFQYFILKFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSE7</accession>
    <name>A0A8S1PSE7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1290079</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSE7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035777267" description="GOLD domain-containing protein" evidence="9">
        <location>
            <begin position="17"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="26"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8106056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="213" mass="24541" checksum="4FAB410F26079D92" modified="2022-10-12" version="1">MKYLIVIALLINLNQCISFYAKEGVEKCFSDEVPSQTIVVIYHELMSEGVAKNEKRDIPKMIKDGITLNVYGPDGNIVKTAKTIEGKNKMSFTAKTMGRYKFCVIKSKQFWSVNEYKYSIKIQQGVDHNLQDAANKTHVESIKDRISALKNRTDDFISLQQLNREQEDKLTTQSIDISRRVTQTTILQIVVLLASGVYQIWSLKKFFKQRFLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSQ3</accession>
    <name>A0A8S1PSQ3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1300112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSQ3"/>
    <dbReference type="OMA" id="PYMHELI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="435" mass="50558" checksum="55B8E95A9EED3421" modified="2022-10-12" version="1">MNYNNKKKQAKLSCCQNFLQKINCCFNKKQKQQTQPLIINDKYNSPFALYGMPNSLCTAKVTLHNFLLKKQGEKIKNTSKDIHQIFIDRQLLNLSQLQELIPYIFPYMHELIITQEQLISVLPYCVTQYLAKRITSFTKQLIVDAITGPGTLSIEIAQLNAFQVDSYSIDQNDNAKYNIQQFHYNSTQRQNIFQNSFFDRQETADAIIIQYQFLNSFQLDQQLLNYLKKAFQLSENIVIILPSKIQINDFCSFLSPFQHKNDHCFRQIEIQKINVGGLPLCQIVYIGFIKDEEIINEQLMNCYLKELLSDQPNLEDIQISHYQKLLRNVITEIGMQKTMLLIEKTLATKTQLDFIINLFLQQIAQLNIVSLENINNQLECSIMDQTPTLNHPLLNASQSSLSKKQSSLRISSDLSKPSFQQSKYKIFSFQNKTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTW9</accession>
    <name>A0A8S1PTW9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T1310021</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTW9"/>
    <dbReference type="OMA" id="MKMIIVD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000557">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001606">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="113"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="410"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="448"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="484"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8106502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="552" mass="63015" checksum="96E14173A6C8E311" modified="2022-10-12" version="1">MGCCSFKLYTDEKAITNSGNEIDEVKEGLEFNQQPNDHSKIIVQDKETSFIKSNSLSKEQGYSKDLEQLQVQTKHKEGDTVEFVKKTISGTMKLTPEMLVRKQCITEKFLAHYEIVKKLGQGGFGEVFLVKHLSTENLRAAKVVVRKTINCEEKLLEETEILKALDHPNIVKVLEIFADFKYYYIVTEYCQGGELLERIKTITNYNENLAAKYMKQVFSAIQYCHQKNIVHRDLKPENILFDSRDPDANLKVIDFGASEKMIDSSFLTKKIGTPYYVAPEVLSDQGYDEKVDVWSCGVILYILLIGRPPFKGSSDIETLRLARQGKLNTNSERWLRTNEQAKDLIMKMIVVDPKNRISMQEAFNHPWIQNIQQNEIEDINLIKNLSQFTAQNKLRAAIIQFISVQLVNKEESSKLFQTFKTLDQNGDGVLTKDELMKGMLSADVDHLNAEIMANGLMQELDVNESGKVDFTEFISAALVQQQKITINNIKLAFKMFDMDGNGMISKSELESIFGGIEIDNQAWDDILQKCDLNNDGFLGEEEFIKLLENIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PVH4</accession>
    <name>A0A8S1PVH4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1320127</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8107061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PVH4"/>
    <dbReference type="OMA" id="KCQFKAH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="233"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="267"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="301"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="335"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="403"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="445"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="479"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="513"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="547"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="581"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="64"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="123"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8107061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="726" mass="85204" checksum="7571787DCEE544B4" modified="2022-10-12" version="1">MQEFKCQFKAHQDEPIKGFCINMNCLQNTSFCLKCLQKKHINHQDDCLAFNPLSNLINEYIKTHNKLINQQIELELLMKQYQKKMEQYIKILEQMDQKINNQSYQSVQEQISFIKYLKWSKTQNKQRSLIQELNNSIQTLKELTKEESYLQQKQNNTAKYKQQIVSQSEKKQIEYNQKDQKFLQKVQKKRQEISGKQLLNIQKQSEASNLQIDSLKKDIRSDKQFHYQQEKIFYNILTIGNSLQNLNLFQEAIDCYDQVLFINPNDYNALNLKGNTLHNLNRYQEAIECYDKSIKVNPDLFDAFYNKGLSLQNQNRYQEAIEYYDKALLINCNDFDAFKNKGLILHTLNCYKEAIQCYNQALFIQPNDYDTLKHKSLSLYDLNQYQEVIENQDKVLNIDPNDYDALYLKGLCLNNLNRYQEAIDCYDKALQFNPNDFNLLINKGINYIYNVGNSLHYLGLYQEAIGYYDKASQISPNDCKALNQKGISLHYLNHYQEAIDCYDKIIKINPTDYDALKNKGNLLHDLGYHQEAISCYDQALQISPQDCEALNFKGLSLDYLNHNQEAIDCYDRILNTNPNDCNALKNKGNSLQELGCIQEAIDCYDKALQINPNDCDTLNLKGLSLYDYKRFQEAIDCYDRTLMINSKDLEAVNLKGQSLHYLNICEQAIKFYDFTLRNNPNPNYMKYKADALLKIGKKEESKELYIKALNAGYDKDVIENIMKIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYA5</accession>
    <name>A0A8S1PYA5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1340030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYA5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="52" mass="6270" checksum="6DFCF37DB1A5A49C" modified="2022-10-12" version="1">MDQRVLKNQKRKKLLLQVQLKKKSNTITKLTLSWMNFGVEQKKEFYQVESQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1L5</accession>
    <name>A0A8S1Q1L5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1400077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1L5"/>
    <dbReference type="OMA" id="YKEIQHF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="225" mass="26560" checksum="E214B3D1452BAD3B" modified="2022-10-12" version="1">MKQEKTKIKQKDQRDEILPINQIIQKCEQTLEITQFEQQELKKMFSIVSDKMHQVNSKIQTFEREERLLRRENDEWEFYLKEKTALIKQLTLLIDLISGKDHSFEIDISTNPVYKEIQTFSLHKKQLGEDLLKTIQNDETSIIKLQAQLQEIEEETKLLRQSGAFWNCVRCNITLKEGFNEETCIFHPGKLKYFSCRTCGGDEYFTCCNQCRDCNPGCKKGLHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q273</accession>
    <name>A0A8S1Q273_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1400045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q273"/>
    <dbReference type="OMA" id="AKQPHKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035934783" evidence="2">
        <location>
            <begin position="20"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8109242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="386" mass="43258" checksum="9931293907E0684C" modified="2022-10-12" version="1">MKTIILALALIVLVSSTQADVIDTIKKIDQSPFGRTLFDTIWLELQTGDPLDRLVSTLTDLEDRYVAEQKEDDAKNQEYQGACTVDISAFDKDLAESNRKKIELEARLEGQLYPQRSILEGLVAQKSAEVKGYQKDVSELDAQRAEEHEDFEEKVLEHQEATAIIAEARRLFADNIEHESFVQKGKATKQPHKFTKDVANLIQKHFSTSAKKTAKFQHRKGYSKLFKAFATIASKVEQLADAGAVQKIIDLADELLAKISDSLSLLRFAEDKRVESYKKSRNFIVIALNVAGSALANAISDLASLNDIIAQVEASLDTTEQRIENVSADRHDRFTSCEEAVQDYQDARSARSSDRDVVSQTIGLVNKELRTLREQLAKRQQAGEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q478</accession>
    <name>A0A8S1Q478_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1420134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000146">
        <property type="protein sequence ID" value="CAD8110037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q478"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="118" mass="14447" checksum="653E02A015D8604C" modified="2022-10-12" version="1">MKNDFRKLKILSFSITRQFLIFNKQEFVISVDFLIYIFNWMSRDLIINTLFNLFVGFLNCEWQNNWQYIKFQVCSNFRQSQINQMFDSWQFNKIHSLVCSISPQLTHMIFHSFFCSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5F3</accession>
    <name>A0A8S1Q5F3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1440073</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5F3"/>
    <dbReference type="OMA" id="ERSVDKP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="190" mass="22185" checksum="8EA872DFC498419E" modified="2022-10-12" version="1">MRNDEQFLSFMVENDDSQLVVESKLFKDNDDDYFKSLSKQFYGFKSQDVKKQLKIIKSAGDLKMKLSNTANLNNVLKPFSISYQLQQQIHKLLSKPKPQGSSLSSSFKTNQKMNQIKLTYQQLQNRKLLIPKNEDSNILTQNSYRSTLNTSTKSQPKKDKVLDLYQMKLKTERSVDKPKIMKVLKRNKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5Z7</accession>
    <name>A0A8S1Q5Z7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1430067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000147">
        <property type="protein sequence ID" value="CAD8110201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5Z7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="70" mass="8559" checksum="E208192FC40B035F" modified="2022-10-12" version="1">MESVESFKQIQELELSKSKMSYFYFFHMFYNQQLQSIFCRHLDSIYILFQQTTGRYGIHRKLQILIKVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QAP6</accession>
    <name>A0A8S1QAP6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1510083</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000156">
        <property type="protein sequence ID" value="CAD8112443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QAP6"/>
    <dbReference type="OMA" id="DGFFPWG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="2"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="181"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8112443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="32097" checksum="4B5616C6FFAA609D" modified="2022-10-12" version="1">MTDYLKAVIETQLIGAFRVAIIMPLEHVLDRIKTYKQSRLGISYIQSFKEIKGARGYLGLYDGFYPSFLRNMFKQYYRWPMMIFIPSFLKEHIHNSSLNKILTGISIGLFESCLITPFERLKTLKMTTMSTGFGYLKYITLDSIYVGFRIQTTRQIVSWTNYLYWDHKLRYALKGDPSQKLGIVNVLIASFISSVLNIMAVHPIDTLKTLVQMESNQAYRKIPLIQAFKLLFQQHGLSGLYAGWQARIIAYFCQAALTTPTIDYLERNYGIAKRNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCW1</accession>
    <name>A0A8S1QCW1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transmembrane protein 107</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1520136</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCW1"/>
    <dbReference type="OMA" id="AWFILDS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036038">
        <property type="term" value="C:MKS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904491">
        <property type="term" value="P:protein localization to ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029248">
        <property type="entry name" value="TMEM107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34341">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34341:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14995">
        <property type="entry name" value="TMEM107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8112777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="137" mass="15524" checksum="8312A7DD438E3952" modified="2022-10-12" version="1">MSAAEILVPAKFVSQMVQLIITSVIYQSQYENLLNFPNDQLAESSLQAGVTLSVIFLAVEMIILATGWTMNFEKTMLIQILLHSLGSVILAWFILDSWDYNYIWAIFSLFCLLPLLLECSTIANAILFRRSIIRTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QD91</accession>
    <name>A0A8S1QD91_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myb-like DNA-binding domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1540031</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QD91"/>
    <dbReference type="OMA" id="WTCIAKE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF276">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_121, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="10"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="65"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="65"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8113094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="40264" checksum="B65E2538E0F9C18C" modified="2022-10-12" version="1">MLNESIYQQKKRILWTDEEDKLLKQLVNKYQNERLGWKQISKALRKQGYERNTKACRERFFNYLDISFNKSELTQLELDKLFELVKVYGNKWTCIAEELNHRTDQDIKNKFYAHVKKVFRRLLKASFKTNESSLITAKLQPLLISSIFCQENEMDQKQIHIRDDMKQLFKSLITQNKSIQQGHQINEETREKVKLISSYLEKENQIYLQNKITRKENKLKTKIKQRNQKFKFIYNEIEQQNILNKIQNKQSIFVLPIKTIDFPFMDCNTNFENVYNQIQQSKIFENESQHQIILSTPFNWQYPSFKIDSFYQPELFSQTIHSDSYNYQTIWNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QHH3</accession>
    <name>A0A8S1QHH3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1600011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QHH3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="206" mass="24983" checksum="85FE55B05BA8AD36" modified="2022-10-12" version="1">MLPKYLNYLPQFKFRNNKNKLKNTYLNIEFLNSNNLDKFYKLEYFFNSPSYFEFRFIIKNICGQIDYNKYDDLIQLQGPPQQFDCTYHKEKFEIKPNETVKALKITLNEYNILNFKTNEQNMQQIQQIKFGPKVAKVCRIGCKRVIDWCKMLPLKEDEFNEVLGILDKSFCDYLVYLENVQNQRTTFYESQLKNEIEKKFKNKRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QIG7</accession>
    <name>A0A8S1QIG7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1650058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QIG7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8115599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="84" mass="10125" checksum="ABAFB4158569B159" modified="2022-10-12" version="1">MSERTLDFIDQRNQVRKLVTWPWNFVFGLQSFQTVQHIYNFTARTIFAASSLIPFLTTYNTHQFAYAENSVRLTRYRNYHDDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QIX6</accession>
    <name>A0A8S1QIX6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1650061</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QIX6"/>
    <dbReference type="OMA" id="AYQMIQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045899">
        <property type="entry name" value="ATL71-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46719:SF7">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL71-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46719">
        <property type="entry name" value="TRANSCRIPTION FACTOR C2H2 FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035876633" description="RING-type domain-containing protein" evidence="3">
        <location>
            <begin position="16"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="408"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8115602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="508" mass="59233" checksum="A83F9D21870AB044" modified="2022-10-12" version="1">MLFLFIFTLLIKALAIDILTINQEYQETGTIFKLIESQYQVIQSVQNTTFYFQPFQIDQDKYITVELIFGYPQDGDVNIEFSITKNKKDNTSSKNDVFYDINGQFLKKAYQMIQINPFTFNSNDKYYIDILIRDTHFTDYQYIIKITKTNNKKCPNDCGNQQSGLCYTENGICACSSEYVDLDCSRLATPLSLKQPLKNIAIQKKDYFYFERDSLISNITLNLGLMNSQYHVSDVQAYIMYENFECGLANEGYFNYYISFHDQSSQSVQIDLSPLIYDQYIERYQRLLITLTTETPALIYISLSLSSTDGNLEMDQSLVIVYVLVSIAILLLVAWIIIILIRYRRSSRVQDETNTISNSQINNNNNINNRRNKSILNTNLLDQYMPKLLYSQILEMPEIQEQDVQEACSVCLIEYQKYCICRLTPCHHIFHSDCLQQWILKHENCPICRTPLDQKSLKELIDKNANQTFKFYQTTNKQTNQNAILQSQLFSNAQTELRIIAHNSIDNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QMS9</accession>
    <name>A0A8S1QMS9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1830010</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000192">
        <property type="protein sequence ID" value="CAD8116899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QMS9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035097">
        <property type="term" value="C:histone methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000228">
        <property type="entry name" value="COMPASS-like H3K4 histone methylase component WDR5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="215"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="257"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="298"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="340"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="382"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="424"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="466"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="508"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="550"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="592"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="634"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="676"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="718"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="760"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="802"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="844"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="886"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="928"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="970"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1012"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1054"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1096"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1138"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8116899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1252" mass="138501" checksum="B1AD40647C8246E7" modified="2022-10-12" version="1">MDDPEDLSEIFAQVQGVDKRIYSLIIQMQEKQNTRDWMGFLSDYENLRHLEEQILQKEDSTQEEKEQRMDVMRNNIKLILNVIVKIKDHDFNKQDYSDQRYLGQRQDLIKTIREQQEIIGFVKFLVHLTAYDENLIQCGSNSLNLLVNMKVDIRNQNFENIRIKNTSLFGANFFRCNLSGSQLDNVDINGMNLNGALLLNCRWTNIKKQGEINKLDGHTGSVKSVCFSPDGNILASGSEDKSICLWDVKKGQYKIKFDGHSSLVRSVCFSPDGTLLASGSYKIIGLWDVKKGQQQNILNGHRNKVQSVCFSPDGTKLASGSKDTSIQLWDVKTGQQKKILNGHSQTVQSVCFSPDGIILASGSKDKSIRLWDVMTGQQQAKLDAHKNTVKSVCFSPDGITLASGGWDEFIRLWDVKTRKKKFKLNVYSQYVMSVCFSPDGTTLASGNKDNSIRLWDVKTGQQKAKLDGHTHSVLSVCFSPDGSALASGSYDRSIRLWDVQTGFLKTELDAHNAAVWLACFSPDGSTLASGSNDKSIRLWDVKTGQQKAKLDGHTHSVLSVCFSPDGGTLASGSCDNSIRFWDVKTGLQKTKLDGHSREVNSVCFSPDGATLASGSWDNSIRLWDVMTGQQKFQLYGHSREVTSVCFSPDGTTLASGSWDNSIQLWDVMTGQQKFQLYGHSSQVTSVFFSPDGTILASGSYDKSISLWNVKTGQQKVKFYGHSSYVYSVCFSPDSTTLASGSCDHSIRLWDVKTGQLKSQLDGHTYSVYSVRFSPDGTTLASGSMDCSIRLWDVTTAQQKSQQDGHTYQVLPVCFSSDYTTLAFDSGDKSSCLWDVRIGQQKTQSDSHSSQVNSVCFSPDGTILASSRPDKSIRLWNVHTGLQVTKLDGHSSTVWSLCFSPNGNILASGSTDKSICLWDVKTGQLKAKFDGHSGYVYSVCFSPDGTTLASGSEDNSIRLWNVKTGQQKAKLDGHSSYVYSVCFSPDGTTLASGSWDKSIRLWNVKTGLQKTKLDGHSSQVNSVCFSPDGTILASGRADKSIRLWDVKTGQQKKKLDGHRNSVISVNFSPDGTTLASGSSDNSIRLWDVKTGQQKTKLDAHKNSVNSVCFSPDGTTLASGSSDNSIRLWNVKTGLQKTKFDGHSSFVLSVCFSPNGTTLAQSNSDNSIRFWEGKTGKEIQSQDKNYKDILNYFKTLLIENSPFSGTYLNILLISQQSIFQAQDALVLKSEFVNQLGINLYTLFKQKGSCIFESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QSB5</accession>
    <name>A0A8S1QSB5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1980012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000207">
        <property type="protein sequence ID" value="CAD8117420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QSB5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="120" mass="14374" checksum="CD3C83B7D97C01A2" modified="2022-10-12" version="1">MMGVFQNLNVILIELTKKILSYQFQIKESKQWKQLDIIEQYKVLLLYLLLIQLLSCRIDNLIVIISQNIMKEWNTIQKLVLILYRNIELIKQNTRVIIAKQNQKICINLHTLYRQISCDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JME0</accession>
    <name>A0A8S1JME0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0040076</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="REATIMR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000568">
        <property type="entry name" value="Calmodulin-domain protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="25"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8042861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="331" mass="38732" checksum="FB97F0D2A19A8F30" modified="2022-10-12" version="1">MDQNHLKLNFSQFIVEKQNSYTDDYKLGGVLGVGAFSQVRKVTHRKTRTIRAMKVISKSRLSTAELQQKFINEINVYKQLDHPHILKLYEFYQDEKNYYIIIELCTGGELFDKIIEKGSFSEKEASYIMKQIMSAILYAHNQNIVHRDLKPENVLLDITSQGNYNVKVVDWGTAKIFSPNQQINEKFGTLYYMAPEVLRRNYNEKCDIWSCGVILYILLSGTPPFNGRTDAEIQKSILNGKYSLEGEVWDIVSQQAKDLVTKMLQYDVQKRINAKQVLEHNWFQQQHQEKLDKQVVQGRLKNLMXTSKLSKVPFISIWLYKIELCQFEQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN32</accession>
    <name>A0A8S1JN32_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0040435</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JN32"/>
    <dbReference type="OMA" id="NRQFLTY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="132"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="42235" checksum="B9552A85D89CDCAF" modified="2022-10-12" version="1">MYYPQPYYPQVYPQYRYQPYGVRPQVPQQWRPPVYNAPQYPYPGLRMPPPTYPYNPYTSSIMPPQYQPQPMLSQIMASPQRQFQSPDRLKGAQSPSRNMLMQSQQVPKSPAPQLQSPNRQYLTYEQVQERIRNAQQQQPQPPQNQKQYTPQPKPNQQKQQPVASKQKQQPTQPPKKEDDLDKRYQNALKSTEEIIKKYQMDDSGSKGRQTNQENQEAEDGELQELALQYEDGFIYRGQGYPPQTRSGFGILTDSEGQQIYAGYWKDNLYDGQGRLTNLQTEELTEPIDWNNMTTIGNGWASYEGNFCQGKMHGQGTLILTNNEQYFGEFEDGMVHGDGEFTNLDGQVIKGKWDQGYLIQLSEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNF3</accession>
    <name>A0A8S1JNF3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050538</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="TTIVEIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="209"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="351"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="600"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="838"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="931"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="986"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1147"/>
            <end position="1237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1481" mass="175323" checksum="267D3F9A92DC1C55" modified="2022-10-12" version="1">MNQNNREQTIVKLQGHLKGATITVHEIEELLLSQEHIIWMQQKEIDDLKKRLKQLESLDIQNEQILHFQNINVQLKTQIEFLKSQIQEKDALIAQFTLKPLSGPSTEMLAAQQQLIQQNLVLQNIIGDLQNQILNKDGVISQLKTQESSLDHLIGQLSANIQIKRSQVGSAEKQKDIQNALNELQTYPEALQEVVQRIIQEYKRISFLIDEKEIEINNLRARLQMQQQKANDSIQEIIKRNPSSFEESRANELIQQNATMEDAISDLERRLISQENRKKELQQKLIEIGTDILQLEEQNNKNNQFNVLDEFNKTEFRQSQQQQPNLAEMTFQQQTKDQTFQLQCVPTEQQIDELVSQNQQQDDLIVELQRKLVEKQDQQEEIQLEIDKIDVISLMQELQKLNQQCDKLKKDIEEKDDQLKLLEKQNMELLNVSNELKQSIKIKQEQIDQLYQEEQEKLNLQEALQKNIEYQRDIQQKQQQIDELKLQKENVENELKNVLTQMDDKNIKVEKWNQLQNDQNQKAQIADDLVQQILELDELIQNLEVKLAEKKDSQSQIEKELKDINSKLQTIQESTQIAKTQRIQLENDFNQQKLCGEKLMDNIQELLVNIKNNEIQLEKLKLQEKEQQNIIKELQFNIEKLTEEENQLRQYKENCNIQNDQIQILEKNIEENKKALFNLKVENNLLQQSMDEIEQEHQKLLSKKLNIESEISQQQMITNNLAQHLETLFSDIKSQEDLKQEKEQQLKQLKTQIDSIIIEDQVALLQEKLESQREQNALIEEIERLKNRFENIKQQQQNIELLIENQMNLNQELSEDLLKFKLIGNQLESELHSLTAQQEEMKYQIKEKSSQLENLNEQFTKIDEEGQMMEAVLSLKQKELKQIQQKKENFTKELDQITQEIFDLKKEIALRRKYQQQQNKVDLNQNEDDIEDEIQGKLNVLNTKNNDLKDDLQKLVELSPIPQEQKLNDVSAQISQFNDQQAQKILKDRDKRIEILELTIQTLQQQNHEKNSMIPSLVSKITQQQSFFLEDDPILGELSEKFTKESQLLLDLQNKLRKTAERHYDLSVKLQEWAEKENRLANELNTRTQCVEKIEQELQKVFDKQSDLESDQVNILQRIYQLETENQELLESEANTKNKLKEKKDYIQVLYKNLVEMDQKLLQLRNRMALYSQEGRQLAELVGNQENERENQMSNLQTIKEELEQIELEKKEKLLLIQSIKKEIQNDTQEKEKLELEFITLHSKNSQLKAQIGFEEAVYQKLLQEFEIMKKKDQIKYQNLYKDGCTQIEYEQDQFESLINEMQFSKITKEEVLFLKQNCQQKLNLLTQSDGNQQYFNNNQILKKILHLQQLLIKCQENENKILIEHNSSRSSVLGFRVLDENSQNQTFTLEQIDQFQYKTTIVEIHLNDQCLIIESTNNKEQKHLQQQKMARQWILDKHFDSNNDLYPLGILHIVFKVSIYQVINIALTGTKVLQLYQLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNN1</accession>
    <name>A0A8S1JNN1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0050046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNN1"/>
    <dbReference type="OMA" id="YSNCENC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000057">
        <property type="entry name" value="Cyclin N-terminal domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000281">
        <property type="entry name" value="Cyclin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="340"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="604" mass="71440" checksum="AE0FF5D51AB96DB1" modified="2022-10-12" version="1">MNLQWNPYFIPKENISQLNNISFMRFDENSQQPLYNKQIQQHKKIQIQPTFQPDCIKYKRNKKQQSCVPFQMNVPQQTSNQTLTKVAQLSLKPIFSKPKSIQQNIQNENLKGSAIWERKDFQNQQITFESFLKEQNLSRNNSIEHNSNKSHFKKNHRQYSHVVQGNSGAYFMISNIQQNNNTNTTNSYYSNCENCNSQREDRKIETINVPKFFIPEDNSNQFSERTIKKQSPNNLIKYKKNNRCISMGEQKQIGLTLECDESSITIISDNINTVIPNPTNFKNNNTLINENNNQNCQIGGFSQETLQVLMLQEIEYAPNCDYLQSIQTQITPLMRAILMDWMIEVCSVYLMKRDTYYLAVAYVDSYLSKKTITKHELQLLGTASMLIASKMEEVEAKNPKDFEKASNYGYSLDKIYEMEKEICKTLQWHLNIPTINLWIEFYTNQWDNYISDVHKKFRQNNNCSFKMILKLQGYIDCCYLDFETLSYQTRKVVASFMYLVLALEYEQFTKEYIYYEIPKTTKFILADSQKSFNKLFTEFAQISFGFNLVDLIEIIKYASKFIMLDFKYSDTFQDKISIQRHEDLLIYQKYNQTGLSFIRYRLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP36</accession>
    <name>A0A8S1JP36_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JP36"/>
    <dbReference type="OMA" id="TQSICAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="841"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="863" mass="97603" checksum="0BEE25BD7B5C504F" modified="2022-10-12" version="1">MNVPNQFCEFTDTCKSFGNTPKICKQGFNKYSCQNVTEQICQFSNNQCSILTTYDSRKECSKLQSEQNCKASPFHCFWQGTVCVFKDISCYKKQTENTDCYNFEYPCGLTSCTPTKPLSCVQIYNRLACLDSDFFCYWDNTSGCQNYYSTQTSINCETITLQINRKVCQYYASSSCEYRDYVCTSNLSDIFYELNPNYKVEATLVPIQLDCSENLTEFDCVTMTQLKCYWNTTCKSIEGETVSCSSKLNLLACQEKDCLWRQRKCIYKQDYIFQPKPFLDQQVTECTTQYISKVTCLNISDISCTYDETINQCKNVTFEDVKTCSDYQLINKKTCQMIPTKSCIYDSDSQACKDFNGTITDLETLSKTACLSLIQAAYWDNGCHLTDNFLCDQVFEVTQATCQLVKSSCIYNQELKKCTSEFNVNSLFCDSPGLSKQACTSIIREPCIFVEGQCKILPSNYTCEQARNVNELGCASLEDSCSYDILNKKCKTVTNSQVCQIPGLSKSACLLNSSCIFNSDFMKCQCNIVATQSICANKDQIKCGEQQLCMFDNKYNSCRRVRCEDLGQIECKNSRLNMVCFLNNGVCQSASKCEDIVNVDHSICSSITFESGEKCIGINSRCLNERNYELYCQLSDCSSKFCKKSNICEAIKCSDLTNCTLLGNKCVKSGDQCVDNISCQLLSSSQCIQIQLYNTQKCLIQQQNIYQDELICTSQVCALYGSSNLCNGNQFNNFTCLLKDSQCLPCEFIQDPCTCSQAKNICQWGKDSCISISCSSFSTAESCNEITRCSWSSFYNSCQLHCNLIISQEDCNIRQDECYYDLANNVCQVGSYNSPDLSVNINISTIYELIIAVANLILILYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPT7</accession>
    <name>A0A8S1JPT7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPT7"/>
    <dbReference type="OMA" id="DHIIAYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035828061" evidence="1">
        <location>
            <begin position="17"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="94" mass="11151" checksum="E6372B6373B6CAC4" modified="2022-10-12" version="1">MKIIIVFIVLITIAVASQQEGFDEIKSWRERIQFKQDYEKLQEMIQQVDHIIAYLDTEQGKNLTPKQYEELLNEITTIEEEIKVITSSYKEDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQZ3</accession>
    <name>A0A8S1JQZ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQZ3"/>
    <dbReference type="OMA" id="IFGYCDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035807868" description="Lipoprotein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="20454" checksum="959824C663BA75AC" modified="2022-10-12" version="1">MNFIVLFALFIFGNCDQKEAGLQKVKVQVATVLDGYYKEFDGVLCYSEKYNQFVIYHERSKAKNLKELFPETDFFLQFGMNGNAVGLSLQNFVPQVIINEEQFKSVVLTVKPEVKKGTTLQIAFVSADYEGEQERFIDLLENGISTYKPISETQLNFAAMQFEHKFIQYVKKMHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRN0</accession>
    <name>A0A8S1JRN0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRN0"/>
    <dbReference type="OMA" id="FLINQML"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="380" mass="45358" checksum="4EA925ED97A14343" modified="2022-10-12" version="1">MNKQYPTYIVKETPRSCLENLTQLYEEPLNKRFITTQDETNQSYRDIQQTHPKNELKKNKVVLQKEYDTKCIAYTDQYYSFLINQMLSRFASLESASQAATAQPLFKMLRGVTKKFMFNELEVIFFLHIIESQKWRFDDDPLIQNFIQYFKQDFLSNSEIYNLEGYKKLLLFLVCCGYTIKCFFNESNDQDILLITQHIQSYCNEDFKKFIENWRQTYMNGALRTAPRIINKLFCKLMKLPKDGKQDLQLDYNALVDSIIQLSPPYQSNDSKTEKKNDKLQIKEQQQQQQQQQQQQEIYQQEQIFQSGNFQQNQQLQFSAQFMLPFQSNQQPNLNLFNSLQLNNSDFLDQFKQQDGLFVSNIKQQEYQSQLYNSTSIFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTL3</accession>
    <name>A0A8S1JTL3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTL3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="40" mass="4730" checksum="482AF5886A908704" modified="2022-10-12" version="1">MRFVAAQYTTAKLMSQRDQCLIQNQRSTRLKSNLIQNCNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXM8</accession>
    <name>A0A8S1JXM8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KATNIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0040177</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXM8"/>
    <dbReference type="OMA" id="ISFWNYN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026704">
        <property type="entry name" value="KATNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027859">
        <property type="entry name" value="KATNIP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21534">
        <property type="entry name" value="KATANIN-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21534:SF0">
        <property type="entry name" value="KATANIN-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14652">
        <property type="entry name" value="DUF4457"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KATNIP" evidence="2">
        <location>
            <begin position="28"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="KATNIP" evidence="2">
        <location>
            <begin position="137"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="KATNIP" evidence="2">
        <location>
            <begin position="444"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8042958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="763" mass="90643" checksum="862821A35AE52078" modified="2022-10-12" version="1">MQQQLLKPQSKEHALQILQQGFQMYQQQGHMSFNENVTVIQQPERMSFNDQKIKSHPKVHNVFDNQKKTRNNQNQIEQSIIGNRFEIPILPSGKYLKFNVLSNWGDKQQVGLNGIEVFDSQGGNITRYIKMPMQDEYIEKLKLIDGYQAVKDDKHIWKTKNLKPEIIIDLTANIKISLIRIWNYNKSRIRSYKGVRLLKITLDERLIFYGDIQKATGDLKKFQDNCEYILFTQDTKIISRIAELDWLNKRAEDYSIQILQQTMSMKQIRPNTGTKLQSQNIRQQQQQQQQQQQQQQQYQQQQQYQQQQTDQSPSSKLHSSRIYQQKNKEQEQPSLQQRQQTYYQQLQQQNYSQQEQEIYKPTNKQEKVQMQYPEYISVFDILLEFNNGSKRFLGLNGLILIDYNNKQIHQEEIQDSNLTEYDIQRLFYSNNHTLIDSSNMVWLKQKKIYIKFKQQTKLSIIKFYNCNQIISESTFTGPEIVKITYNGRFKIFNLRPAPCEEINFVQTIFLTQPNNNSYSGNLMANHELFHQNYVIAYPPCGMIITIYLINTWGDNNYIGLNGLEIYDHQGSALLQRKLIPYTIHSVPQLEDDPRVVQNLVIPPYDINCQPQRSFLAPFINTPMEKLKNKNAIIIQKARDLEKQDINKIYIMFDKLTCFSGIDFYNYTKDWQRGVKQVHIYINNRLIYQGNINQTFQDIKKNVFNKTTILFSDSNQIKQQLNPYFYQFPEEQVSLIDENKQLTEREYQQYFPQQLIRPKTSIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYK0</accession>
    <name>A0A8S1JYK0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0100028</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JYK0"/>
    <dbReference type="OMA" id="CINIQWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8045790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="55731" checksum="2B2F734940B0D4A8" modified="2022-10-12" version="1">MFEIPKLKKFIFPIIGTLTIFQYSLQQSMPIHTLDQYVKSSKNPKQATETLILQNGIYIFGCVLGTELYKLLANQSSKFIFLLSDVIYFLGNCTLILLYFQSTNSEDNTQEESEKIQLQPIEIYLILRVFVGIANGLNYAQSIIYVKQMSSKKESQFFFKLIPLQLLIGQCLGAYWLESDQQNTPDIITFLNIILILNFVSVLRMILLQFLQIETPQYYLKILKDQRRSKMIYQEIHNDNIQTYFGFEQETLRNNLNQSASEYLISKPYLKKYFKCCLLIVLTQFTGETLIFSFLYTPLHKYCDSQKENNGCQIYWIYAFITGFLMILLQMIFYNFIKRKKIITLIGQAIICIALAFLGFSVGLKDKSWQLVLLQFGYSLGIGTQIFSIIPYQLPEFGILYCINIQWIIGLVEMCFYGLFLNKLPSSDSLEQIHAIQELIQIYFFVSCVGLILIFIILRQPIRTQPQQKIKKEEKKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0E8</accession>
    <name>A0A8S1K0E8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050561</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0050563</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0E8"/>
    <dbReference type="OMA" id="LAFIWYP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="90" mass="9878" checksum="14AF0E25C8C75400" modified="2022-10-12" version="1">MADKGGAKGGAGEEGKEGEDKEPEKGCCDKYAECLVVTGKAVYTCCLAVCTCIRECLGFIWYPCKERCCGCLDNCDKHYNQWKDEGFTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0G4</accession>
    <name>A0A8S1K0G4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0G4"/>
    <dbReference type="OMA" id="QWKAHCF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010321">
        <property type="entry name" value="DUF922"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06037">
        <property type="entry name" value="DUF922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="220" mass="26067" checksum="58109F2F0BCBA1EC" modified="2022-10-12" version="1">MIIRRCSISNSTSQRSQSQKMSENEQCNSNSHFSTCCSNASSEIDEIDDPLNYASFNDFKSVLPLVINGVKIKKVTWDLFSAPPNVYSQWKAHCFWTVGYTYDIAMKKMKHSNKIRYRLTINAWCCLNSKSWVKNKWDRLLEHETGHYLIGCLCALEFKARAEKSKFTKNYRLESTQIFQDTFQEYLSLERRYDEETNHSQNISRQKEWNEFIIKELQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0J5</accession>
    <name>A0A8S1K0J5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8043948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0J5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="199" mass="23417" checksum="915A900E8FA94E1F" modified="2022-10-12" version="1">MKNNHHRRTKSSGNYFPIQTEFLVQDDDNDTNETKEIIKKIQQQPILHHQIVEKLDDQNYGLFNAMFNFGQMKFKPKQSLDLSTITECSSTPSTKDAKPKHRKCGSQQQQANKQPIRHTHSQSLQIQRLEKELEQMKVQQAEIQNVNMMLIIEIHKSRQELQISQQKNEVILQRIMKLEKMLREDQKLSSTTNDSLEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1E6</accession>
    <name>A0A8S1K1E6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0110083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1E6"/>
    <dbReference type="OMA" id="KSNIGMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000085">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="220"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8046750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="519" mass="58557" checksum="626F03E242BCC19E" modified="2022-10-12" version="1">MNPVRVFPLKSNEQQNTQSTSSSPQLKKKQTPKLIEKPFLLNMSPILNGQSPIKGKFNVKRLNVNPKQEDKFQFTQQQRPSQSTSSSPIKRVINGATKIESQSPMKLPNLRRADTQLNNNIQIKDETIQEMKSSKRKDTTTDGTSTQQIRGSIIKLISESTPDTRPSISRAKLGSQTIEQDIKTKASNQIVMLHGRSTSQATLVNTFILDNEMQDYVNLFYSKSQAGQNGQGQTKTNQDSIIVTNNLGGIKNRYIFSVCDGHGVYGHYVSQLVKKLLPNIIDQQIKSNIGMQEKDIGENHYNSITKAMTQSFLKMQKDLSNCGIDITFSGTTCSLVLISGPHLWCANIGDSRSILIQQQNNQKWKTIELSNDHKPDLPNEYKRIISSKGRVEPFISENGEMIGPPRVWLLNDQIPGLAMSRSFGDYVASTVGVSCEPEIIHYRMNTNFAFLVVASDGVWEFFSNEEIQKIVVQYWQPNMTAKKLNEICDHIVKLSTQRWHQEDEVVDDISIVIAYLQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K277</accession>
    <name>A0A8S1K277_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">26S proteasome non-ATPase regulatory subunit 1/RPN2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0060512</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FEGMIII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048570">
        <property type="entry name" value="PSMD1_RPN2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21505">
        <property type="entry name" value="RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit 1/RPN2 N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="117" mass="13739" checksum="303E1A4068602357" modified="2022-10-12" version="1">MNFNTLLLEKDNDLRILALKEMLNQVDQHWSEISELLSQIEALSEDPKFPERQLASYLCAKIQYHLDNFDESLNYALQSGQYWQSDLXKNLLDIKESNSIILAYFVNQFFTFISTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4I7</accession>
    <name>A0A8S1K4I7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0170003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4I7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="669" mass="78155" checksum="221286E3BE969DF2" modified="2022-10-12" version="1">MENYQDEQKGVEQIQQLQFNYEILLKDANSNSNSSIDELQKKSSIEKSTRNSIVTIDYQNHVNQLLKQYEILNQFQLNNKVDGKNVNINFQNFWVIEQIIVDPQIAEYSLKIGLATLLLLKSNLKGSLYCLSKIISFRNAKITQQLLDLDWSLLYLDELTEENLEFLIQNSDLKCMQQLLFSKNKIDLILNIKSDYSNKILKKIFTCLDSYCFNYNYAEILYQKKVIDKILKYLSVANQECFQLIGSLCLSQNAQNEILETDIQKKLFQNLKSKNSQIVANSYYALSGICFNQSYLQLKFVNSDLPKFMKSHLSQASEVCIIDSLSQLISNLTYNNDQVKQILGDFIPILIEIVLNFLQYLKDKKQQKEQYFKGIKSLLRALCNLIFIKTNAILACEHNLAGLCSFLYSNELIITEYLDLLINLGIHQLMNQQLLISLDNLIQTQQYQEHLIKIIDSIISSAKQFSKFICSEFNLVTYKNILKQQTENHQIIQKIYKLVSILLSEKQNFIYLQECGLIDEIKPFQHINIQYYYEKIQNQLQEITHMSRIFGNLDEKSEQLFDIKVVKGNNKLNNAILQIKKDQISISQNGITKSKYIIQKLKDILDLEIEQKEFKNILQIKNILRYYCLGLIGYSKSQMNKIIIQAQNQQQKQKIFQLLQQFISDQVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4N9</accession>
    <name>A0A8S1K4N9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4N9"/>
    <dbReference type="OMA" id="MTQDNST"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="308" mass="35210" checksum="C11E747187CA780A" modified="2022-10-12" version="1">MSQLTESPNRLNEQHSIPQDEIIDKSQKLATSSPDKENSQELFRLQKQQFLQKMTQDNSTRRQKKIIGDIKDVVNVNKQIVNFGLVIPGNICEDDIQFQNLSTETVIIGIQVICNNTEFDDLDEYVYSARKLNGYDYNDRFMLACPAQKQFSMKIALKVPNIKEQKGLFGTIIITASYSNHSKIQGQINTQIQSQITLPSIECPKTLMNSAYNLPVIQMAFKLGKKLDCKIPFRNNSLIGLPLEMEFLNKNDNEILINPPTLSVQGNSQFLVTIYIKSKQEQIIKNVLIVKVKNSNVHFSYPFIIKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4Z7</accession>
    <name>A0A8S1K4Z7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4Z7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="116" mass="13429" checksum="C2AA5E1C205ED219" modified="2022-10-12" version="1">MNQINYLSFQFENKFNFFFKLNQIQKDIIRVYSNNKYKSGIAKSAKGVKFGFGKNKKALQFRFLKAGLLFLVSRIHRNSKQSISAKTEQGLQAVFTSTILEYLTAEVLELNYNKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K535</accession>
    <name>A0A8S1K535_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K535"/>
    <dbReference type="OMA" id="MNHMIDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="145" mass="16907" checksum="B6AEE45F48B88C54" modified="2022-10-12" version="1">MHQGSFISNTPIGQLDDFRKEVRGKMNHMIDQPKQIYTKSSNLQQLLRHSISPKQPKNHELNKSQDSSNQYNQISSRLSFQRSSLDLGKPTQNILYPKIQLQDILPLNKILELQRAINNYNYPTNNNYFIELKKLAKIVLRETQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K560</accession>
    <name>A0A8S1K560_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CNH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0090444</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K560"/>
    <dbReference type="OMA" id="MFVTSEG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032914">
        <property type="entry name" value="Vam6/VPS39/TRAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019453">
        <property type="entry name" value="VPS39/TGFA1_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894">
        <property type="entry name" value="CNH DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894:SF27">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10367">
        <property type="entry name" value="zf-Vps39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CNH" evidence="5">
        <location>
            <begin position="16"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8045488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="926" mass="107702" checksum="330B06CFE7DD6F12" modified="2022-10-12" version="1">MKFGSVFNPRGVLEINYKISSIDAYQNVLYTGDEKGTLNRYQLQPDQAMIIQANQAQSKSLTKSRIDQIKVFPQANANLLVLSDQTLYFVEEKTLKGEQISKEKVSLFALNEFSKINQFEIVIVTKKKEGFILQYNQKAGKFECMRERFLLPDIPVTISFIGNLFYFGISKKNYSVINLDDKQLQVANLIMDIGNNPYIKATDNDEILIITTNNIGIFIGKDGQMKQKSTIQLQNKSIQIITIFKQYLIVLFDNLIQVFNLLDSKPMSDIQLPSSAKCITQTSNHLFYGSSSEIIYLYQTPPEQQIQDLLKQGKVEDALQVFQQYNQNSDASKNQQLEQLKLDCGWALIRQMQFQNSLNYILQTNFEPRDFICLFPDYYYAVEKLESVNQNPSQNTISLIINKYVAEYNKGDPKKSQELKLQARDFLIEILEKKRSILTSTQYAYSMKDKLTLLTSTQIYNQNQWHAIPCEQLLELIDFALIKAYLEAQQLSKLKSFLSCNQIYCLSMYAELQAIFQNNKAIEQQQGILARFYESFNKIDLSLEVWRTIGGDSLNQQVQQEACEETTRILKQNPDKNRIFKFILWVFKKQIKTGIQIFYVSESIISPDQMLKFLDEQEMEQDLKRKLKEKYLEVLVLEKQTEEERFHTQLAYSYIDSLFNIFPKDMQPSQIDMKKNQIAGDIYQSLKKFLKNPNAKYNSSSILEKKVKDSWMIGEVILLYGREKRHEEALSQLLNLGYYDWAEKYCCEYTDNLLTKLFKKYKELYFFLEGKYKERSNDQQAQYAFTQVKITINNFLKKYATHSQLNALEVLEMIPENWILADQGEDDGLFQFLNSVISHTLHQKRSTKAAFHLSDMDLLNVECLNASTKQASVRITSEKKCAVCSRSIGEKVFVVYPNAVIAHHTCIKSNTVCPQTDKDFEKYFKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K690</accession>
    <name>A0A8S1K690_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0140212</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K690"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035716870" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="241"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8049883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="42089" checksum="0A4DA25CCD5006C6" modified="2022-10-12" version="1">MKSLLLLLFVGVLCQNEVDTVEKLLADLKGASAVELTALNTDYTVSKNLKENIIFNLGKAIQEQNFLCASRDKSVSDRESDIDKTNKFIAYLQRRLVDNARRVETLDNNRCTHSSNYIERVKNDNMTLRLIIFLRNSLQNLDNNQLNRYGAYVEKFLTMYKSAKMSELVELAHEFADTMFIDWTQVTPSSQGNINTVKGQLIEMLDDMERYIREQIKNSQNLEITTGVTLADFKGAIDIENEQINYDLSSEEKNIIKLQEQLVQARLAAVNCRERGVALQQQQQRAIEDLKIEEDQYNKNKARLQEELNLFTDVYRIYSTQVGTSEDKFKQRVDDYVKDKQVESYERSEYSLSDAVRKQVQHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K738</accession>
    <name>A0A8S1K738_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0140078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K738"/>
    <dbReference type="OMA" id="HIFSSDM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8049615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="528" mass="61315" checksum="9C0ED41E1AF27CC3" modified="2022-10-12" version="1">MSDSIDFNDMINLNRNIYGTQQQKEESKMIHKPVDDSSLTIDNILKGNQAFYNPMSLQASQLKEKIEEESQEEKVNFFQEVQLLTKIPEIVSFNLIYNQKTLFKNEGIPYEEAKHLDKTRIKRKERELKVSGASVEKEIAPSKKQYVRLYKNQISKICFTAPKNLLLISQHLGNNTKWEKQLKIQDFGGQRPSLSIVQEKTTGRFYIYDNKQGRVHIFSSDMLINDNKDKPLVSLNLNEDKPRQDPLLYLIECEHEYQWKSVLLVIGGHHLQRGEKKPLKTIKVFEIKRREVTLTPVLTITMTKSRMNPIVFDLIKDKGFIWQDKDNGDKEKVDAEHRSVDQKYIFIMGGNPLLEYEKEVDQELIEANQTCEYISLKLILEHIARAKLFSMTDQANLSLNFGKFSIVDTLTIKYQNIIATEKVYNHFYNASVTKMVDDVFKQKALIILGGNTSQTFQIQAISFKLNEITLIGQTKQISKPSVIYSASNLRYFEKKVFYFDETDKKDPSQLQNLESRRDNKVACTCSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K766</accession>
    <name>A0A8S1K766_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K766"/>
    <dbReference type="OMA" id="FAMCSNP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="224" mass="26397" checksum="A5A62660AB5AD330" modified="2022-10-12" version="1">MNMNDSFFDKIEVVNQKLITYSKPQTICKSLERSFAMCSNPPNKIIQQQTQFHNNDTAISRNQSYKLDYFLKQQTKSSQNNTQSKSYRSIRQQDYNTNNISQNPQKSYLKQQMRLKINLENDAYQSNLRHKTSSSHNTTNPYQASLANLMKQQMQKQQQSFANICKTLNDESKPRIPSTHRQSIIPQKLTQRQDSVNIRKSNSVYMQEFRKVFSKSKYSFHNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7Z2</accession>
    <name>A0A8S1K7Z2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7Z2"/>
    <dbReference type="OMA" id="QFALMHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210:SF1">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="1">
        <location>
            <begin position="132"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="1">
        <location>
            <begin position="349"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="453" mass="53375" checksum="29F0B8209ADBC9E3" modified="2022-10-12" version="1">MNNDNTNNDENLNDIDVQLRRFGIQFYALGFNQYFYLDNPEIFRGDSFGEEDDFLRGQRQSQRRDRHSPREKYQNLQKYLQKRTEPIQNDHLDQHQPYIKVTSFRNMNVNNEYFINEFGLIGSLKNTNSKDILIGRSHRNQNQDLIPNDIILPEDRIISRIHCKIVCKDYFRKNQILDQKYQFALMHINLPILIKHKISQFLEEPKNVYIQDLGSICGTYMRVFRQEPFILSYDTKFSIGSDTNFNIVLNETYKQNSKEVDQEFYKILKRLFCLKSQQKHEIHFQDKQKSQEFYQLEEETTSLSAKELYQKLKEYNVPILGIKFSGQGVEQNKQQNILIGKSNLDTDEFYIGRGADNSIKINSNTISRKQCRIKYSAQYQAWVISDGSQDRDSANGTWVNLSTVEQSEKKKESNQIVLKQNSEIKIGDFILKVELVKGQKGRFGQQIKELLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEA7</accession>
    <name>A0A8S1KEA7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEA7"/>
    <dbReference type="OMA" id="NQAFIYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="421" mass="49740" checksum="47DF0C96EA3E6D57" modified="2022-10-12" version="1">MIQQERKDDLRLKILLKSSVKVKTNNLCKFIDKIVPLNENQAFIYYDAGSYEIYDVESQQIVGRGQISNDVKYFYQIYPQTLCFLDPEDMKVKFLDLKTHQLNQSFAFAASNVVKQNSDKQQKETLADKFRIMILDRESEKIRYFITQDLEKQYSNTNIGHFVIRVIPQNYTSDTQFDMNPVFEYNYPNKTGQTELYNWQVDEETFAVFGQDFELGQAEILLIKPHLLDKAFYIIQIKDTISYTLINVTNWIHPHLIAIWQNNNEGTAKPTIFTIDIRQYYDGQNWLPEPEYNPIKVRDDYVGDEPVTFVDIYQFQLNSTYVLQFRLVGVEPRLSLINYANIDKPELINEVKINEDFFHEIPSNKSYLLQYNDLEIKDGLIEFSIVFPVDARHQLLQIIEKAKLIDKYGINNVEELFEFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEP4</accession>
    <name>A0A8S1KEP4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ABC transporter family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0210282</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FWNIIVI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000610">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000140">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000151">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002273">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="780"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="823"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="908"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="156"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="481"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="783"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1099"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8054070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1365" mass="157485" checksum="99736546C2E00294" modified="2022-10-12" version="1">MSEINQQKKHEELELTSQQKDSATNNQMLTVLNDQQDEQQQEQQPLNHQQGQDEPSMVSIPEEEEEMFYPYYHGQNNWFISKLAFLHLTQYQLFLKKHLLDKGHKIAQKHLPKLSPWEDLKETVKQSQNTVKQKKNITCGDLVKITFFGQLKWITLTCFFAYFMEAIIRNGISIVMSKVITSAGENEIDNAYGYATLLVFLNLLCVFTRHHGYNLSMVFSSKARMTLINLVYIKLTELSAYSIKEANIGKILNLISGDINYLEFVLIMIFQSSVCFVSIIFGSYILWDRFDGPIGMISLAIIFIAYPIQIILQSFNSETLKTSKQYQDQRLKLTNELIEGIRLIKMYAWEQAFHKMISIMRKKEYICLLKISFRSVFDRLFSLISQVWSSFVFFVILYYGGYRKTMSVAEMISTIQLLTFFKISCVFMVSYGIQSVIHIKVSFTRMATILNIQNSEMTNLDKIQLKTDNNNQQQNINDPRIQLKNFTSFWTSKVTETTKPVLKNLTIDIQAGEAWAFIGKVGCGKSTLLSAFLFEIPAYKGSFKIDGQEANKGKLVIAYVEQEPFIFPDTIRRNILFGRPYDKVLYQKVLHASQLETDLSLMKFQDHTEIGERGTTLSGGQKARLSLARALYQQADLYLFDDPLSAVDANVAKNIFHMAIKEFIFDFQVQRNPSKKKPIVILVTHQVQYAVECDKIAILNDGELIAQGSYDDIKQSLYMINDELALQLNNTKEIKKQEFVKPSFQKRVKVRNSVANNLIGKEADQQSLITLKTYFRYYQFWNIIVIICILCLEAGSEVINNFYQRIISLFEEYQKENDIDTAYFLLGMLTLALFLTNMMKYILNTYSVQSSTQTIHQQMLKSIILAPISYFDVNPSGRIINRFSNDLSLCDNQTNTVSLDVLEIIGNFLFALITLAILQPYFLIMIIFIITIDFYQFSYSKKIISQLKEVELMQRSPLFDFLKKTLGGVIQVRVYEQQDWFRKQFLDISNKCNLNSLTYYYSSRSFGFNLDLVGFIAQTVGIYIFVKLNYDNVAILSQGLLLLTTYNDALQWGLRQLITFETQMNSYNRMFQVIDILPEAPHFTKEDEKHPDFPKDGRVKFENVFMRYRQNCDLVLKGLSFEIKSGEKIGCVGRTGAGKSSILQVIFRMSEIENDYESKLLISDLEIRKLGLHKLRSNIGIIPQSPFLFTGTIRRNLDPFENYTDEQLWKALEQTDLINHVKAFQSGLLTDMSDVNSVFSVGQKQLICLARIILQQRKIIVLDEATANVDMKTDDFIQETLKKKFSDCTLITIAHRLNTIADYDKVMVVSEGQVIEFDTPFNLLANSINSVSVDKQTEFSRLVKNTGDQNSQAIFDIAKQKQIRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIC9</accession>
    <name>A0A8S1KIC9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KIC9"/>
    <dbReference type="OMA" id="QIRWARV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="45"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="178"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="529" mass="61864" checksum="BD37C641353BF059" modified="2022-10-12" version="1">MTSFSNFDEKTSLINLCVKTLQIAPSRRTQSDLALLLQLVEAITFFKNLQSQNPDIVLKCMTVLSYKTVKKEEILFHVGDSGSLFYIILKGSVGVYILLPSPEDFKKFELKEVNILKAGNSFGELALLNDNAKRTATIIAKEDCMFAVMEKHHFKTILGAQEQQKVQDRISFLCSFPFLQSWSFREIKTISYHFEPIQVTLNQAVIKQNDYCTNFYIVREGNFEVTYTLNDQQKQKGIFSTHSICVLGPGEFFGEEAFLMDDGKTFILKLFNIFQIRWARVKCSIICRSQCGQIFRISREDIIKRFWDEKTQTVFLSLLQSIHQFRLNKMSYYDQIASDKLKQLNGQEGIDQFVKPMLKVKVRNNCALIRKETQQEKSDQEFQFYKKQEKKAFEQFKKLFKDRNCSYAQVKTFFDQKTIGSQNYIDRDSYFKLFFRFPSQLKNSEISQSLNQPQPSTARTQYRLTTQPKEENEKSPQFSTKQRTLLILSKLKLNEVLPKCKTIHNNNSPLNIQRLELRRFGTQSYLQMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQM4</accession>
    <name>A0A8S1KQM4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0230217</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQM4"/>
    <dbReference type="OMA" id="IMTISES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035883520" description="H-type lectin domain-containing protein" evidence="3">
        <location>
            <begin position="16"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="H-type lectin" evidence="4">
        <location>
            <begin position="36"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="584"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="455"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="772"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8055512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="833" mass="97338" checksum="3B59B865D8127741" modified="2022-10-12" version="1">MKLIILILIFTSVMGAIFEQGYVSSSTISAHKIYRQDIKFNNNFTEIPKIVLSVIGYHSNYNTLDFFSRVTDITNEGFQLQVISDCEINEIRFNYLATEHKDVQTICNNIEVQKDQIDIKFITAFEEQPQVAAFLTGIHRKNQDDIILEIKSIDKNQVHLTTQSKAESSIGLCLLIGPQDVFNRKELPIMQISQGLPNTYFYGISSLSGITDKGVQLSLNQQYVENSYEIGLTLIEINPKHISKQEEADDNNKLSFDDLVDKFSPVQQQINTDIFQTETKTKINSIQITQEELDSESQINSISFIYPEQQDHQLYIEDEEQNLQSNQVDVQQIMTISESWLQHDLSVVDQSISQSQESLDSNQTQINEGQQFLTNLIKPQDYNTNSIDYEDYQSLNRQPENIVIVDSTQQIVDENDNKIENNQDFEFDNSNQMDSSIKYDIENEIENQIEEQIDSSQQQTVVEDLQQQMVEMQKEMQNLQQNSESTLSSEFDPFKIIEQQQFIVEQQKQLVEQQQQIILQYQRLFEEQIKQLSDKQPTSQMDVQQVDQELKGDTELQENKNEVTKQDSLENKAIQKENLQKKLEKDYVQQNQEDSQTEEKLQQNTQDQKYVLQDIPNLSLEYKNDLEKDLESFDRLKQKKDQIDEQQATKILKEITKDLQFDDKLEDTINQFEDGLNDVLQIKQRNVIESNLNNEFTNIASNKRVEFSNVLNKISNQNLQDVSNQVKNISLEEISEAIFPQEYEMEQAYIDWSMNQGFIKNRNDQVINQELVQQTIMQNQQSQEELQKQIEEFLELDNNDQQFSDPLFDDFHSFFSVNQQVKNNLRKRKVLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KU86</accession>
    <name>A0A8S1KU86_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0270190</name>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0410165</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KU86"/>
    <dbReference type="OMA" id="KKFRHES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002673">
        <property type="entry name" value="Ribosomal_eL29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12884">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12884:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01779">
        <property type="entry name" value="Ribosomal_L29e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8058387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8067786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="75" mass="8675" checksum="CCFB00B7F8C5F5BE" modified="2022-10-12" version="1">MAKSKNATSHHNARKHHRNGIKKLPNQRYTSLNGCNQRFAKNRRFAIKNDPSIKKNKSLETRLAKRKAGKSKNIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUA6</accession>
    <name>A0A8S1KUA6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0270205</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ASSEEWW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000061">
        <property type="entry name" value="Sodium leak channel non-selective protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000030">
        <property type="entry name" value="sodium leak channel non-selective protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000525">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000082">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000117">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="484"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="523"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="589"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="655"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="824"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="863"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="895"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="957"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1060"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1143"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1173"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1203"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1271"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1364"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="98"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="450"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="824"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="1144"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="720"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="780"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="725"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="780"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8058417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1572" mass="183978" checksum="A3D46BB3E0439F5E" modified="2022-10-12" version="1">MSQTLNKLGIEYKEINQIQVEEATQSIKQMRLEQVLIGECENFGWQNLINRHDLIIVKSLEKSMKEQKKAYNQTLFIFSNKNPIRKGITKLIRNQNYTYFITLIAALCLIPYCINEKRLAISIEVVCNIIFGFDILLFIIAYGVILDKKSYLRDCWNIANVLAFIFTWFIFLEDNKVNDVIKTIRLLRLFRLIQEIPLLRIQFTAYVSSFLKLKQVLIPIFLVMIYFAIIGLHLFIGLTERRCRLTPEPVDGVWLADDDVLKLCGIWQCPEELYCGSPVDYGLPKNATENDVAEFNWDFTRFDDFFHSLLVVFTFLNVTGWSGTTFMFWKAMTTYVTATYFLVLIVLLAFILSNLLLALFYESFMEKSSIKNSSSKAKKQEQEIEDDLKKKNQEQLMNRLALMSKQKSQLVSQNQSFNGQLNLEQQFQFDQTIFQQTQQIKFFYSLSNSRNFNFINNLCIILSGLAIIIDYKDISKKTLEQLQIVDFFCIIYSLVEMAILISGRGLKGYFSKDINKFDFFVIFSQWILVFVLVVINEPLIINHNVYVTFFKSLKILRIFRFVYNAKLFYKIAILMRSLVLTLTRLKNILALWACLVILIAIVGKSLLQNKLKEEYMTIYYDDFGSALMAAVNIFYNEEWHVTMYKYARHTYVSFFFSILSVILGQIFFIRLLMAVFLNEFVQQLQKVENELKPIDYKQFIQLIVQLLQFKDKKKKKRIINYQSQKSDEKNQIEEQNNKKEKKEFRKENKTNSNLNQNIPLQELEELQNWLQVDQQNQEQQEIKSNLSNNNNQNEKENPQNPQNPTQSKINNDNASKIRQIVDSFIFQLFSLLIVLASTIRVGMSTPFLDPNSQTSQILKIVNIITTILFIIQIILNVIARGFIKGEESYIQRSPYNALNVLVTLVETIGLFLPSHTIFHFFSSLRILEFIRIGTYLSQSINYISQALVKAFQTMLQLSIFCFIILLIYGTFATKILKGQMYYCSSISEEEEFEINDKWDCMDYGGSWVNRILGYDNIFDSALTLFITATTEAWLELLVNTWSARGVDLTPVNNHNRWWAVYYQVFFFIGNICMLNMFISLVVETYQQTKIKAQGMSELNSNQREWLSIKESINHLKPRRLYEKPKTKVGSFIYDIAESKVTKYIWHAIILLNTFTLALYYNRQDKYFTDALDYINLICTIAFTVEVLSRVIAKFPNIYDRDPFIIVDAIGIIINLINQFSISQMNDEFYLRRGFEALSVAFQVLRNYYIIKRFPELEKLFHTIFSVVPSALSMLFIMFIFLFIFACLGMDLFGYLRPQSKMEGFDLHFKKFTTAMFSLIRVASSEEWWALLIDSVHVRTPDFACIYIKGYEEFQEYGYNGCGTYWAYVFYVSFHLIFSLVILNLFIASILGAYDEHAKQEQSAISKYQLHDVLNYWAQYDPNGTGFLNYKQFWKLSSEIAICFGVPVKDLLDPANKKNFLKALNIPLYEDGEGLIGYLFHDVIVSLTRLSVELKYNVRDLESDTYKGKEQVHQYYKKFKKTPYYSGQMSTMIYLQGKARMLINKRKGIKFLDLGALKQELQNQNEGDKFDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXG9</accession>
    <name>A0A8S1KXG9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXG9"/>
    <dbReference type="OMA" id="QINHKQP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="115" mass="13732" checksum="5AE0297C50018BB8" modified="2022-10-12" version="1">MLDKALTYCLQEETPVSIADIMQIQQDQQINHKQPRILAQPIKLDFQQFQQKFIKQSSIKDMINQSSQEFKQTPKITQDFTPYQSGWQKNILNKYYNLNTGNNSFCNKEEHIRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZ15</accession>
    <name>A0A8S1KZ15_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0260289</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZ15"/>
    <dbReference type="OMA" id="GQSKWIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115">
        <property type="entry name" value="KINESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="2">
        <location>
            <begin position="6"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="86"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8057926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="363" mass="42060" checksum="44F663CBDD11B782" modified="2022-10-12" version="1">MINYNQIYVAVRIKAYLNNQDKEESCIQSFTDKQIVISKSNMYEQQYEFNQIFDSQCDQEQIYLCVAYPIVKDALNGISSNLIIYGSQGSGKTYSLFGTIGSLFTLKQGLISIESGIAIRMIHQLHQQKQISELKIFIIDNEQIINHLMTNGQSKWIELAKQLCEVIQNLNDSIIHVVIEIKTLQNSKIKIIQIGSISKGYSGQMVTHNKSIFTLHRCIQCLIDQQKQKKIIQMQRIYIPFRESKLTNLLSNSFLGQAQLKIILCLAPTKIDDALGTLQFGQQFQQIKILKQQSKHQTQFSVQQQFQTQSQLQLNEFNSMTTQSQTLEMIPIQFHLQKIQELTDKYESIIQYLQQKIIKYKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZZ0</accession>
    <name>A0A8S1KZZ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0280122</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="QGDIRIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="601"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="642"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="713"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="766"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="436"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="884"/>
            <end position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8058942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1250" mass="149965" checksum="560042BA607A73AF" modified="2022-10-12" version="1">MKGYCFQPSSREIIENLQTNNQSPHIELVSTTHRLMAMSTYQRPKKIRRNTTISIFTQQNESESPKKSQRWLFMLRKYITIVRISISFINSLKEYQNKQKIERLDSKQWKKESLLPFYPDDFFIKKWRIFIEILTFSSVILYPIYINFEVNKVIETVVIVFDLFFLIDIFINFLTGYIDECNNLIIDFNSIFKHYFKTWLFFDVLSVIPAINYEKEWFKKFKMIRVLKYFLNNKEVTYKGQENYVKLIESLNPEQEFTLKSGTKYLLNVIITSCLLIHIFGCWQHYFDSGDYITNIYWSSQTITTVGYGDINAKYEFAVLWMVIGVAFYSFTIGDFALMMQKSKVNAEQDLLFLIEQLGHAQNFSEKLKHKFQFFVKSNLYHNQFWLEEYKSMAKELPHNLKLYLTLSTMLDICKQIPLFLYDINYTSLLLLNIRYLIVEEGFILYREGQSSSEIFFLLQGDIRIMTKDKTILLNILEGTIFGEFEAIEEKLRGTYCVAQRKSICLVIPYRILRRVMSQSQILDFEIRQLHLRRRRLIINNFIEERRRKHAYKRQQSFLQMTKEEYVLNKKIIQILSVEEYNKLXSVILYPIYINFEVNKVIETVVIVFDLFFLIDIFINFLTGYIDECNNLIIDFNSIFKHYFKTWLFFDVLSVIPAINYEKEWFKKFKMIRVLKYFLNNKEVTYKGQENYVKLIESLNPEQEFTLKSGTKYLLNVIITSCLLIHIFGCWQHYFDSGDYITNIYWSSQTITTVGYGDINAKYEFAVLWMVIGVAFYSFTIGDFALMMQKSKVNAEQDLLFLIEQLGHAQNFSEKLKHKFQFFVKSNLYHNQFWLEEYKSMAKELPHNLKLYLTLSTMLDICKQIPLFLYDINYTSLLLLNIRYLIVEEGFILYREGQSSSEIFFLLQGDIRIMTKDKTILLNILEGTIFGEFEAIEEKLRGTYCVAQRKSICLVIPYRILRRVMSQSQILDFEIRQLHLRRRRLIINNFIEERRRKHAYKRQQVRFYTQSFLQMTKEEYVLNKKIIQILSVEEYNKSLLSKIIGKKLQRLSQFLVKFKRSVQRVIEMNHQLDETIPEQWRELSNYQLIKRVFPKDYLSQKFQQISLSRKSSSVHSSVSKLSKMKYSTYSQQYFFQKLPEIRLKHIVLQHIRLTKNKFVPLQQIINKYSEDPEQLNLKKKESVERSVWTKKFTKISQTVILRIDQIQDSGDKYNEIMQSMKKIEKLWLKGSMYKFEWFQDQHSQSKQFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5E6</accession>
    <name>A0A8S1L5E6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mei2-like C-terminal RNA recognition motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0310310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5E6"/>
    <dbReference type="OMA" id="HPYYIIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12531">
        <property type="entry name" value="RRM3_MEI2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034454">
        <property type="entry name" value="MEI2-like_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007201">
        <property type="entry name" value="Mei2-like_Rrm_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04059">
        <property type="entry name" value="RRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Mei2-like C-terminal RNA recognition motif" evidence="2">
        <location>
            <begin position="168"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8061435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="298" mass="35303" checksum="707D6B374ADFAB85" modified="2022-10-12" version="1">MDKQSRLNQEQILRCIRNHRDLNEILTKQKDHSSQELFDDIDKATMNQSNESKQSQESKSNSDKIDRAIQMFTEPKVILYAKEDNLSSDLMMEQISEVFKNKSFDQKVGSNLSTEISNEPCLQLGSSCQSLFTSGSETERRKRKTISEEESHYFVVKIDKVMNQTDQRTTIMIKNIPNKYTVQMLQDLIDNRHNNYYDFLYLPIDFKNKCNMGYAFINFVHPYYIIQFYKDFHDNGWPHFNSEKICELRYARIQGRQALVQHFQFSSVMNQKDKKLKPVIVPQSELSRIHQLIQRQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L762</accession>
    <name>A0A8S1L762_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H(+)-exporting diphosphatase</fullName>
            <ecNumber evidence="2">7.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0340229</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.3.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L762"/>
    <dbReference type="OMA" id="KSAWLLY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009678">
        <property type="term" value="F:diphosphate hydrolysis-driven proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004131">
        <property type="entry name" value="PPase-energised_H-pump"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31998">
        <property type="entry name" value="K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03030">
        <property type="entry name" value="H_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8063257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="171" mass="18031" checksum="4B0C2FF75BDB855C" modified="2022-10-12" version="1">MVEEVRRQLAEKPGIRDGKEKPDYDRCIAISTKSSLQEMFAPGLLVILVPLVLGLFFGPSAVAGLLPGILVSGVCIATSSANSGGAWDNAKKYIQADLCEIDDIIKGKGTDEHKAAVIGDTVGDPLKDTSGPSLNILIKLSAIFSLVFAVVYDKSAWLLYVMKTGSSGCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L8F9</accession>
    <name>A0A8S1L8F9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0320210</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L8F9"/>
    <dbReference type="OMA" id="SPMDISC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003579">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051570">
        <property type="entry name" value="TBC1_cilium_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN 3 WDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="474"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="660"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="804"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="683"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8061863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="838" mass="95365" checksum="288B5CFAB9F7A005" modified="2022-10-12" version="1">MDQLNSWGYCNPTNWEDKLPKPYKFINKILNTMILNEVYNKVFEIEKYRSDPNYEGQLRTLPPQGVFDIPQLSCVSRDSSSNLVAAGDTQGNLMILDLSKKLRIAKKETNGKRILKVCLSSRDQATDDYKNVCVIGVVQHNDPVVYIYRFRPLENKLLLHHTITMSKDKNVIGEYPVDVDISQFSQYITVTQYNGSVKVFRIPDLKIDQQSNSSQISNNSVGAQSPRHIQSQFKPSPIKGKEQPIVAPIQQQQIQTSPMDITCSELTDLVYQVKFNGIQKTLDYNGIIAKIKQDFTTPKEPPPEEKVDPKKKAPVPPKKAVEIAPVPVVEEILYDENDNGAVPNDDDYPIQKYRALVEFIIERFSIQNGNKTFNSYKQHECITGIVVGWTNTTRVELHRFTSAKRSALPEYLSASFGLPSQQLLKQQASITEVQILYPLSCMAISKSSVYLAAGLQQGSVFVYDIILEQERFYLDKHMYNCTQIQFCDDNRLVSSSYDGSVNIYDIKEGKLLCKRTHQFRKGSKVKKEEQKQGLWRIIGMSVSHTGVAAALDAQQEVRIYDVWHGEKIGKLTPQQVMDDKLRQWVQDKPLVACYKNEILISADVLQVNQYTTLQIYKIFDNLVNLFPGLANIYRKGIEKDKVMNLFEKISKSELQNPQFDIPNLLGNGNNQLKIPGQEHRPSQQRGSQQRIGSQHGSHKSGKLPPAAPSSIKGGPVSLINSLHKSNHSDQQVDFNSSFKSYQASSMSHRLSKQNSKQAILTKEMLHPEQFLLEKDKSSIPVLIKDDTSMVERCRSRNSERLVRIEKVNNMIQKVGQQLAQEEEKKKLQERHRQLSSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9M6</accession>
    <name>A0A8S1L9M6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0360015</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9M6"/>
    <dbReference type="OMA" id="NPQHRFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24162">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013881">
        <property type="entry name" value="Pre-mRNA_splic_Prp3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027104">
        <property type="entry name" value="Prp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010541">
        <property type="entry name" value="Prp3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212">
        <property type="entry name" value="U4/U6-ASSOCIATED RNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08572">
        <property type="entry name" value="PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06544">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor 3" evidence="8">
        <location>
            <begin position="155"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Small nuclear ribonucleoprotein Prp3 C-terminal" evidence="7">
        <location>
            <begin position="442"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="106"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="190"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8064217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="562" mass="65307" checksum="DAA2BFD3F3BE52D5" modified="2022-10-12" version="1">MNKNKGVSKDVQTPQFLEKKPQNLNPKLKQSDLNRKLFTMPIDAKYKAVLGPDTASLVAYLKQKKKKEKAIQKLKDSGKIQIIKEPKQTIDKSLPQGIYETEEGKLKDISGKIIRLNDQAKSLKINQERQKLDKIQEMLKIQNNLKDALNDKGRFLDKNVTIQRSNLKRQKRKLLAFNFIENGNIDKEQVQQTSVQIQDEEKDKVQVQEKEIQNLTTTSVQTNFKRVCVKMKHHDPIPNIEWWDVPLFPEYQANYIPINIESEQEHKQPATAHTQEEIQRNITFLEQLQYTDTNILLDKITKLLEHPKPFVISELVQNREKAALPSFLTKKERKKRLRKIRMDIQKEKQDKIRLGLQKPSPPRITLQNMMAILGAEAQIDPSKAEQEVRRQIQARLDKHIKQNEEKKLTKEQRGDKLKLKWQKDAAQEIRVAVFRIDDDLSSTNLESKKLKFKVDMNAQQVSLQGVCLIANQNGQQVVPSIVVVEGGPKGIKFYKKLLLNRIKWRCDESKPGCVLVWEGVAKQHAFNKWRTIEIKSEAEGKRILAEKGVEQFWDLAMNNHQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LD95</accession>
    <name>A0A8S1LD95_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0360163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LD95"/>
    <dbReference type="OMA" id="HTKYKRV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="399" mass="47764" checksum="E9A36D16E88D41DA" modified="2022-10-12" version="1">MNQQFTDIINDQLNPILGEFISQTLTSSSIINSPLKIIRQSNVVEIQNVHTKYKRVIKETIQQIILEIEKYYQQLIEKNQAIIFKLYQRIAEQTSVIQQLQNLVNQLTQENIEFRTKKPEEEITQEFYDPQQYTIRQQKKELLELYDISDQLQNKLKRQESINNQLLDTIKRQNSQLSSQQQSITEQQLMIQNQKSRILEHQKYQAELEQQIIDIQKQSQKIIERQNDQERIEIHSSKAKYLTLNQNDSIVSKFNKPEIQNINNKQLNPLKNLLTTDQETETQPSQKCLFQKRQKLLLQSLEYQNYSDFEISVKNRYQSKNTIKKTELFDISEIIKPKYKKKPQDLDELYLYSHRYRNSSVDHYYEPPKQYRFNIKKTLISPLSQRNTDQKRIGLCTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDE1</accession>
    <name>A0A8S1LDE1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0360183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDE1"/>
    <dbReference type="OMA" id="DMNKDQP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8064551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="324" mass="39012" checksum="2888787B9A889663" modified="2022-10-12" version="1">MEFKKEKPKATSQRFKKITQKLGIQSLNKEERFDEGMKDFHNLDTFFTVTDLLKDMNKDQPTPERQALFESQQTLIKTKLKLKKWKSEYPDYLKFEVKPPQTQYRNKVVLLQKAQTHLNLQKYEPQLYKEVENLSCLLKDKFKNVDMKEYPQLQLFQEISQYEDLRVRTEFNESLRDSYRKDRLSLKLEQFEQKPTVSYDTYYKNYHNQAEQLRNRLKQQQMNFFNQKWGNSVVKMKMKEAQDKRGFIEKAKQVRRQAVTKKVVLPYLAPIIKNIITDICMEEAEMMEQKRNLKNEIQELAKVCSDFYDPKPEPQIMGLRIINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LGS9</accession>
    <name>A0A8S1LGS9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0400029</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8066937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LGS9"/>
    <dbReference type="OMA" id="FKVARFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17364">
        <property type="entry name" value="MFS_PhT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="475"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="28"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8066937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="518" mass="57414" checksum="A843F2E4043D5C2C" modified="2022-10-12" version="1">MQKDHLLEKKEALDALNNAKFGWFHIKACLVSGIGFFTDAYDLFIIQLAVIMIGMVYYHDEVRDGDTIIKKADKLYPVSETLLKQSALIGTLIGQLLFGYLADLLGRKKMYGTELLIIVFTTITSAFASNTASPNGLTVVGMLIIWRFFLGIGIGGDYPLSAIITSEFANTKNRGAMIAAVFAMQGFGILTGSIVSLLVLLSFNNQLNNGDPFNYLQVDHIWRIIVAFGAVPGLVAIYFRMTIPETPRFTMDIKGDVEKGTRNIQQVLQQDDTTFADSNQEEKKFKEQKPSFRELKQYLSKWKNGKVLLGTAMAWFALDVGFYGINLNQSTILKQIGFGGGSNLTQYEILKQAIYGNLITSALGTVPGYWLTVLFVDKWGRKSIQIMGFVALTILFLVMGLCKDILGQFLFITLYTLANLFNNFGPNATTFIIPGEVFPTRYRSTCHGISAAAGKLGAIISQVWFLGLGADNFKAIMLTFAIFMMIGLAFTFLIPETKGKSLEEISQGQEQSNQIIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHQ8</accession>
    <name>A0A8S1LHQ8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cystathionine gamma-lyase</fullName>
            <ecNumber evidence="4">4.4.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Gamma-cystathionase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0410104</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHQ8"/>
    <dbReference type="OMA" id="YGGMITF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004123">
        <property type="term" value="F:cystathionine gamma-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000008">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000009">
        <property type="entry name" value="Cystathionine gamma-synthase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF15">
        <property type="entry name" value="CYSTATHIONINE GAMMA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8067668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="395" mass="44103" checksum="63309E97BE2AECFC" modified="2022-10-12" version="1">MLVQEEDYLFDTEFTIATKTIHKTCDQTYGAVNPPIYMSSTFELKDVDSFGKGYFYTRCGNPTRTVVEEIVAEIEQNNYAIAFSSGMAAIQCVINLLKEGDEMLAMDDLYGGTIGYLKSIAMERHGIKVKFVDMTNIDEVKNQMNQNVKLIFLETPTNPTMKLTDIKELCQVVRQIQNNVIVAVDNTFATPYLQTPTELGADITLNSGTKYLGGHSDIIFGTLSCRNKELYDNLFKISYLTGGCPSPFDCYLVTRSLKTLEVRMKQQIKNAYILAKYLEGHPKVEKVLYPGLESHPQHDLARKQMRAGGAMISFYIKGGAAEAEKMMKGLELFTMAVSLGGVESLIQIPAKMTHSGLTEARRQELGITQNLIRLSAGLEHVSDLIKDLEHALKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJD2</accession>
    <name>A0A8S1LJD2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0400010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8066899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJD2"/>
    <dbReference type="OMA" id="QYCTISK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="154"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="190" mass="22544" checksum="AF1DB410A21ED0EE" modified="2022-10-12" version="1">MEKMYNLNNCYLNLKKNKQGKSQLKHSDDDHKFVLKQRHETLSDWMSKKTIESERVPKPRAKSQYCTISKIQKQPTTSFYDSYKQVFLKFKLKPRIHNSNQPTQLTSNAFGKINDSTYISKSIDYADLQTKSTKSNKKRTPIISRNNRQFQAIFERMKKVLESKKRKEKKLLQQIAMLQSEIVSLKQQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LM71</accession>
    <name>A0A8S1LM71_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0400277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LM71"/>
    <dbReference type="OMA" id="VENWICI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818">
        <property type="entry name" value="DOMAIN-CONTAINING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="117"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="260" mass="30934" checksum="FA6A636A904E77C4" modified="2022-10-12" version="1">MDQSEYSYLQCPPEAMAIQHDQLYIRKTEKDKQYRKIFGGDVQYDQFEMQHIKLLEEEIQKKGIKLPDGWTQTDTLKIVYNGKFKIKDCIERLQLHLAWRVNPNMHTLNKQMQEFLEAGFLYTFGRDYQFRPVIYLEAFRVDQKKYPKEFMIQVLSFFLGFVKKHMMMPGKVENWICIIDTKDIGVFGLPSGLGEIIKTMALNFVGCLHRMYELIYLGKLVLHLWMMKANLKIFFNQENIRKIKGSYSLRLIRKKVFGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LP59</accession>
    <name>A0A8S1LP59_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0420033</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CERIDCH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1187"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1224"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="752"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="794"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="836"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="878"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1345"/>
            <end position="1379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8068195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2273" mass="267011" checksum="ECB09BA544C2BA8A" modified="2022-10-12" version="1">MNTVQEVIISESEKQNIIYYEDLAFQSNYNEIIIYDDKKQNLGESYKIDCEFNKYIRCFSFSTNYQVFAWYYNYYNQEEELIFYDFKEKKKIDNIENKGRVKKIAFSPCKPVLSVIYEDGNLYFWNISKSPFECQKFNQVDESVQIDQIAYSPDGSLFIIYIKYDILRIFDEIKGSEIKNKKLEFSINGDILVSNDNQTLALIKSYYNYDVILWNFIQDKEILKVENQSNYPIIQFCPDGIYFVHLHLKDKNLRFWNKNTGDLIISYQLPQESSYNCIHFLKDGKQFVTAGTYIRIWKYSENKIEMISAYNPDGTVTESLLTYNDQNLVCVVDGHIKIIPLQICNLKQIIPTQRKIAQFLTNGYLVTNYYSVEIYDWKNAKSIAILDTDINTVYFFQDSQYCLFFKNKSIMKIRIDSKEEIFSLNICEQIQSIKLIKNEQILIIECKNTLKLFNLEDSSNIYEIQTYVNNNLDYFYWSINNDYICYRPNTNFLIREIKTKQQNNFISLENKVLQINSFDSEDQILLKDESGQGILNLVSQQFNLFETQFKQSTNSQNDQDIFCERIDCHPKLYEIAHNTYLITFDSNRILPQQLKKNGQFLYTYLNNNQVTILSIDEQKQLKIQCSLQFESEVKKLSVTPGFDYITVLYQNKVKLINLDWLLQVQMVSIYEKKDDVKDISSFIISQDDTKIITGSKAIRVYRLDLKKEKHDYKNLYEQINCFVEIDQNTICIGQEKNIQLVRIDIYQKEIILGKHEDAITCLSYFQRTQLLASGSKDQKIFLWNVDARKKIAVFEGHTQWVNKLSFSPDGKCLASASDDNQIKLWNIELSEQSKISKGHQQYVNQVTFSKDGLILASCSYDNSIILWDLIEKNFIIMLQEHTSYVICLEFSFCSKWLASGSYDGSIFLWDVKFPHETTLFYKIQEMYLYPDHLCFTPSGCFATISNDDGQQYKKKYYKDDILNVTKIQIWSLNKIVKEEKKFLIQRNWNDSFFISDDDLVFQGYQKIVKVYDLSTKTSQDLEEHQNQVKIIKSWDYGQKLLSVDDDNIMFYWEKINKTWKKQSTIQLEPNITQIDIIRYDYQDFIVTKNEKSIIISDLNQLKQQKPFIDLNIKSSIKDVYLSTSQNFFIIVNYDNEIILIDSVSGQSKNTFINCYNTRQVKLLVIILKLRFMKSLKCKSKQQLIKKKMLTFQILAGMIQIYYIQHQKKKQLKSVTLKIDLKKQFLNCSILIIMVSFYLDYAIIQFMMIIISLLLQQIQVINKSINXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGLILASCSYDNSIILWDLIEKNFIIMLQEHTSYVICLEFSFCSKWLASGSYDGSIFLWDVKFPHETTLFYKIQEMYLYPDHLCFTPSGCFATISNDDGQQYKKKYYKDDILNVTKIQIWSLNKIVKEEKKFLIQRNWNDSFFISDDDLVFQGYQKIVKVYDLSTKTSQDLEEHQNQVKIIKSWDYGQKLLSVDDRNIMFYWEKINKTWKKQSTIQLEPYITQIDIIRYYYQDFIVTKNEKSIIISDLNQLKQQKPFIHLNIESSIKDVYLSTSQNFFIIVNYDNEIILIDSVSGQRKNTFINCYNTRQVKVSNDDRFLAISDQSQIKIYEISEMLESVVIDQEENVDILDFSWDDSNILYIASEKKIIKKCNIKNKAKEIIFELEYSFDYSSYFQISRQCNFLICKGFNQFTLINLCNKQEYQHSIEGRAEFATFSYNENLVAISTSYYKIYLWNYEKQIITENKLIYIQTTENLTFTDNDKELICCQSGSICVYDIQDNLELKLKYIWEIESGRFCTYCPKHKSIFKSYDYNIAQIYIVNYAQTKILIDKEHDPINFHSTQYGDYIASISQQSLIIWDFKNEKQILNTTQWCGKQVMLIELKTVIIGGYKEIKILDISKLDNIQEIQTIIFEQQITSLSFSLKQEYFVCSFDYKIKVWRILNDGQSYQVAIYDTEYSGQTNPVISASGDILVYNEQENLKVIRIKQLNTIELIFKTVNGLQYSSDYQYLITLIDGCPQLFTSNLEKVENQLEGSLQDIKGDFIKSTSVDSIFAIADDSCIWLVQIVNKNKLTQIRKIMLEIEGKIVSLGINSLGTLLIAGSNFGSIYFWDITKSNNMDNPKPNQIIKNQNDAIIYIAFSPNGTDFSAAVFDGSVNLYSVEQIKNVQNIKQNDDNQEQNQIQVIENLNEEFKLICYKSFSRQSLLLANKCKIKNSQIKQNEKSILQLFKQKGADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LR75</accession>
    <name>A0A8S1LR75_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LR75"/>
    <dbReference type="OMA" id="CQSTDRV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="148" mass="17481" checksum="8DC5C21A3B1D10A9" modified="2022-10-12" version="1">MECYTQLDQKQALQVYELFNEYNKLTSSVRQFGFISNSFKNCNISQPKWYIPTKREQDELQLYFQNVKIYLTSRTKRQKIEKSASQVLRVCQSTDRVYRKSTSFVGQKGDNFLQNTTLGYMKKISTATNPIAFQYTFESESKTEIQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LS92</accession>
    <name>A0A8S1LS92_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0470068</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LS92"/>
    <dbReference type="OMA" id="EFYCKQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051219">
        <property type="entry name" value="Heterochromatin_chromo-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22812">
        <property type="entry name" value="CHROMOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="3">
        <location>
            <begin position="5"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8071268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="192" mass="22898" checksum="4B34A18E4FAC1CB0" modified="2022-10-12" version="1">MIGLFVVEAIVGMRKNLSKIEYQVKWLGYSKEENTWELQENLVQNCSDLINQYHQNEFFQKFKGLIANYSEIKGNLRTDKPFQIVREFETQFLIAYYPRKNQFVGSSLINKSEAPIDLLQEFYCKQEEDIGQQEYQNQQIGCILDYYEKQSYVVLLQSKERIFVDNQIIIDRQPEIMLDYLENMNWITGVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LT82</accession>
    <name>A0A8S1LT82_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0460128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FIKMAIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1352" mass="159507" checksum="57638CA41EDF7A5A" modified="2022-10-12" version="1">MGICSSKKSDKEAGIQLQIEKTVAEQKQLKIEEELQVQHSTRRSICNQFSTLNTNGNVITVHQTELEVQKNEPQQQQPKTSKKKARSTKDDVNYYRRSDHVLKVFSLIVELTQDEQKKYYNITIGTKIKDCVHYAHVNGILITQADFDLFHLKPDTFKEANKYSRIPTFFSTDNYFNIDKESPNSFISFENLLNVERIIQLKQIQMKLKKKQSLLQEQNTNQQNQNDYVMTSNLEMLEIITVVDIEQQSRFLNVLFHLILQHYHQNLDQYLVFIINTTFLFDIPPQFDQPNMYRLALFYDKLSYSLKDLQQILLNDKSELSNLLHKKLSLNLIQILINCYEKYLYRLNFTLSTLFYVPKYKCFKLESFGRMKTLIQFGQNPEVALKTLEEKKYKAYVKSFKKDLFAITDLIIYFKSVQNQNLRTIITTRKKYISNMKKEQGENLEDEKNYCLLAERLFSDNQDRYLLEFIKMAIYSCEVTDIDKLIQDFKQVSDSLTKVLIEADYQQESKQLDQLDQNPQQLKQSNSVDLEDLNEVKQLESFPINICEDYKSIALNKDKISEVEQTYMEIIYQSLLYSNRFQAYYEKFQYLQKPIFQNCYVEILNIFYFIKQYPDDSWQEKLKVVSLKMDKVVDLIKTVKDDSVDNFHITLLFQIMTLSQKKPLVILNQLSKCISNQLQQKLQMDHRLRSQNQAKGLLEQKKHVMLQVWSALSYIHSDNYFQAISKIQKAIGFQLKNQHQASLSYGYSLLVSGEIARLSLAPISAMLNLEMSLVIYNQFFPEYKQVEDQQDIQDRSEYNPFQIKYKDVRVKNLATSNKAKVEFLLGMSYFDIHDQENSLLCLKRAQELMLRSFHIYAEEVVFLVLQQLWIHVLQDDIGHAMNIALFYANDMNQQYRKGREFKKKIISKLQFIIGSIFEFNGQYLSAIRFIERSNRTLTKAKGNNFVIQMHYQAKKISIISKLKQAFLRIKSKQQVRCEQDLNLFQTLNEAMGQSFQILYFKQPITMKTSVLLDKFGKQIIHIQGLVRTDGQSKAIKLLGELIKALNKSKKDEIYGLVQDKQLILTLDKLNSKEIEAQIHSVIEIHDRYLQFPLKQHHVLKCQLILLCVFLKQKNTTQIFEHFKSIEQTIDEFQNILQWKQLVLEGEDIQTQNKFETIVKRGRIGMVYQIANVTELIEIYNKFKKKIFENLQNRDKFVDLANLFQKKIINSQAFQKLLQTKQACLLGSYSQVKIVNEIEQEQQQQVDIINQSPQKVQTIEASTEVDGKDNQKLFFDLRSFDEIMIEKKLAHHRKGQTEETLQKIEEINEPEKMKKSHKRNETEMTFKSAVKKKKIEYKMNPFQSTLKKQQKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUV6</accession>
    <name>A0A8S1LUV6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0440247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUV6"/>
    <dbReference type="OMA" id="KYDQSEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035769591" evidence="1">
        <location>
            <begin position="18"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="397" mass="45680" checksum="21534F45913105CB" modified="2022-10-12" version="1">MKFSIVLPILLIIGCFAADFDQQVEELQNSNFGQTILQTILMELQTGDPVVSNLIDMVQGLETTLENEQERDDKRIARITQNCDIDINTLKDQINQNTVNSLTLKSELDSLNPQKVSAVASLERKNSEINDLKTELNYQTHKRETETITYQVILDNLEQALFGVNQVKGYFNSYLDVLVKNRKRFEKPEPSFLQETYSFKYDQSEIEDTEDQGQQFTSFTQVAAKVNKLKHHVHLEGYRAMLEMMSRLATKAQDEPSQAEVLTRKVLSILKQIENYIQSERIREDQAEALRSGNFELLQTLLSDQLVQASQDRTYMEGLVESLSTRIQQASNEKFEVDQRIVAKTRELENRETDCRLKKNEYDTDTANRIKQKRVVAVAVDLISSKLGQLKRKLLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYJ5</accession>
    <name>A0A8S1LYJ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYJ5"/>
    <dbReference type="OMA" id="WKKYNED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="89" mass="10436" checksum="3F20F714D1A2AFA6" modified="2022-10-12" version="1">MNLQIQSEKCLFSNKNRHQFGPTPQAVKINNKRLKRESKSVMEVSFKKNDQVIIVENWKKYNEDVSNLEQPQPPTKKFKETSCAGCYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZD7</accession>
    <name>A0A8S1LZD7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TPPC8 C-terminal Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0460212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZD7"/>
    <dbReference type="OMA" id="DQICDQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057651">
        <property type="entry name" value="Ig_TPPC8_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024420">
        <property type="entry name" value="TRAPP_III_complex_Trs85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975">
        <property type="entry name" value="TRANSPORT PROTEIN TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24542">
        <property type="entry name" value="Ig_TPPC8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12739">
        <property type="entry name" value="TRAPPC-Trs85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPPC8 C-terminal Ig-like" evidence="1">
        <location>
            <begin position="1088"/>
            <end position="1183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1205" mass="143349" checksum="9B7071CF33173B24" modified="2022-10-12" version="1">MNITQLELFNQACHQYKGMVISSDFIQAALLSRYQITPAEFLRPFLPRKLQRLKLGLREKEKELNIKLLDLKEYDQIGNVKLDKYSRLLLEDTQFVDQVQISRNCSKQVLDQICDQNKMAGFQQLQQVVGDCLGFQYHYNFLDHPIVLFYIVSGQEKNLIQEIHKIRNESNGLIKMIFKNNLNDLDMVSKVIIILNDAQEIVQYESLDLIFKEFSKFRAYTLKYNLPNPIEQTHWSRFSYQRYNYDGEVPKHPLGILDEQWTKIYRQVVEEIILQQAVIILERQINIQTQLYQEMKQKTFDTFLTSIFNTRTGQQTETEYKMSQREQLLRFLGDNHFLMNDYESAATYYKGLITELKNNKTNTTIATLYATEYYLYSRLLAQQKIDNLKNIFDEIQVGYHKIVHFPYMLRVMFFYIITIHMFGKYSKEQIYFLQRFHRYFKDLGSTTKKDYFVVYQILVYEQIAFIHLRLDPPEFRKFAHNLTLVANKYESENFKKHALRCFKIVEQLYQQSKWVPLLFWLEGHIGANCLENQRIQEAIDSFKLAFNQLNERQTLELHNQLVVDWKKAYTSIEQLQDHRRKYFTQSNTNLKIPIVKGPVDMLTHGLDVLDSIVLTNIKQKFKNLWNTVSKNESFFEDASIRSFDSIYLEERITCFNKSPCILNADKITDLIDKINKFGRQCFAEDTITLRFFINLPLKVPSYINGLQVMYQFYTLKENLLDLEANNEKEYILTKINTLEIKDIPFQRAVQEYIEVSITPPQAGYLIITGLQWNFLNIPAQISINYDAKDQSKKKSVNFYNKFKVFPKFIPIDIEYYTKPVIAYGEIRPILFKFTNKNPEEIVIHITPIIPYHFGFEEKKIILSAFQVLEYQFYLRCSFQEEFVGQLLFKYQQGQSGGIRVQKLEIPMKVDPSFKISLQTDLIVDYWKFTLQVQDYFGGKGNLKFDRILCLNENWKYFDVPYQSSGDPFLLTTFKLKKIQTKDYRYEEYQNRLKERFYREGDNFNEIILGDQGIPADVALDFVNLDIQHYFNVFYQKLSNQKMEEFPLFIIARWTYRLEDEIIYGLHELPVLQQNYLHRDVKEFPMSFSITAPFEVQHQSMITMVPIKISFKNTKYVDPISFTVTLFNGDEPLKSINDIQQPFFLWEGILQHQITLKPQEMKVLNLESVFTEKGVYDLSRFRLTMHNKQQNEYYSIDHETVIIKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5Z5</accession>
    <name>A0A8S1M5Z5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Piezo non-specific cation channel R-Ras-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0540245</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="PESIMLQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071260">
        <property type="term" value="P:cellular response to mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027272">
        <property type="entry name" value="Piezo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031334">
        <property type="entry name" value="Piezo_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056770">
        <property type="entry name" value="Piezo_THU9_anchor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13167">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13167:SF25">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12166">
        <property type="entry name" value="Piezo_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24874">
        <property type="entry name" value="Piezo_THU9_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="506"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="528"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="550"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="613"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="656"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="684"/>
            <end position="700"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="720"/>
            <end position="743"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="763"/>
            <end position="783"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
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            <begin position="817"/>
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        <location>
            <begin position="842"/>
            <end position="858"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="870"/>
            <end position="890"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
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            <begin position="1011"/>
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            <begin position="1098"/>
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        </location>
    </feature>
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            <begin position="1303"/>
            <end position="1320"/>
        </location>
    </feature>
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        <location>
            <begin position="1332"/>
            <end position="1349"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1415"/>
            <end position="1441"/>
        </location>
    </feature>
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        <location>
            <begin position="1487"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1513"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1548"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1598"/>
            <end position="1616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1921"/>
            <end position="1938"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1944"/>
            <end position="1962"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2140"/>
            <end position="2159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2179"/>
            <end position="2199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2228"/>
            <end position="2248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2260"/>
            <end position="2277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2297"/>
            <end position="2314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2372"/>
            <end position="2395"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2652"/>
            <end position="2674"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo THU9 and anchor" evidence="4">
        <location>
            <begin position="2138"/>
            <end position="2393"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo non-specific cation channel cap" evidence="3">
        <location>
            <begin position="2442"/>
            <end position="2737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2744"/>
            <end position="2773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2745"/>
            <end position="2763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2764"/>
            <end position="2773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8075517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2773" mass="328777" checksum="B15E8AF57C69B62E" modified="2022-10-12" version="1">MYNQESSMNEIEIPTPNRQNNSQFQSEGDNADLQNDLYPVIKKADSHESLIPKDQVSPGKYQKIMQALDIIGMLGLLVYCILIQSYYSTYYLICLLLILNYSYISSYKGVQVRNSKLYTLTEFASQQEQFHNTNQNQEQSENTFITQYWLVLNDFKKPIWQSFVIVSALSLIVKLVLYPIAKNGGYEMMSIENFSGLEVYFTVINGKITVSSAEQGLAFAPNCIMLALAIIILTFRNVQIKHEFHELSDSPILTKFFILLMLLNLIILTCCTKSLIGLIMWGFQLVLLLRMYQNQLVDKLYFIILKIVIMVILVVMYFISTPIKKVILQNSQAASFTLQFTGFQLFGYFSNYEAQAILFIIFSIIVLNMLCIVNEKIKTQNIKNQFNYTIAEISQQYEDFKQFQTLIKSQMENISIDIDDSPKQKRESGEDDELSDSDQIDLEEEKEISDENNDESNQDESKDNIKPNRISNFILDQQINTSFYINKIERVWYDSLENIKNTIDKPLVSLIILQILSLSIVILQQTYFSILLVCWLILTGFTLNFRIIRYATIFCNIPSLLINFFTFYYSNITGLDRPDNFYQFCPSSEYKSIDELPTTVCQKLGFFQQNNSAVNSILMNCTLCLFFLFIKQIEEQEQKEKQIQTVSQEIDVLPKWFNIFLAFFFRISFLMVIVLLYWVGFNEINIIQFIMIFLLIIFIVNQSNKCEYKGKRIPFQQKYWIILLVYMSVLTLIKYLYILFGVYQSYFVVQILGLNMLNKQLVLNWLLFYLTIIQYYGFNTVLYDRYSNRLIATISQIEGVKDNLPNFRYHFGILETIYGHSLIWTAYVVNICFLLFTPYSYLNNLLLLYIGIVFLYHVKSINFKINYIKLRLLWYIYMYLLLIAFAYRYLFSFFCVPDLNDKSEGFQRFYENLQLYYQEAGLYAYSTDNLRIEYLADTLNILFGAFALNQLSKFKKLQIKQENQNLSESSSQNVLDLKILDQQKWLKSILIGIAHINTDLITIYIVISCVLYLRSIAAFILLTLYLSYFYRVHNKILDILEEQQSLSKLQTQIYLLKQLYLQFASDISHIDEPKKIKRLNDEIKLLRLNTRIKLKRNIWLYSFYFTAFCIFLSYLSQFLSTESFTAIVDPPLWVDYTVLSFFIFGTYSRQYIITNQCYCGYESNNNDCNENNYFQTESNMWQVSWFYLFLLFVNVIDTACTKILEDDLTKSIENLQETQELNQIEESRIQNLSYEESKEIFDQKYDFIIFVKSHSEYNSFDREVLILNYFKNKSRFVKILLYESLFKIFQRFLILLYLSNTLLVNTIFSLFYLIISIILFQMSATVKNTKMLNSIAIFSILFQYFLYLLNWTPSKDDIPQLNNLNISPEDFNIMAKTNLSDYWLAFLGFESTEIMDCDQLNESAFQDMKCETNKLLNIVIVFNCLIVSFIYLYFLMLEWFCNQIYRQSIKMKNEFIICKKVLTNHNDSQIVITYNLWRQKNYSFRHVLIQTITMNYHLILFGFVLLLCEMNRSIFNLFQLLIVIGFLYVAEFHFKWPYATTERRKIKAFYQAIFTLSAIIIALFTLLKVPTFLEQCVNASQIRIDVGNGLEFYCMEAFSLKIDGQIFLIFFISLYFDLQQSNHMKTTQVNYQQKLKLRSKMVSRGLAYNYNYLQYKQILQLQEEKNILNQISDTIEKKMIIWKKRVREIQMKQLEKENSLNIQQQQYPQLTASFMTEPEVQIPTDQSFFRKIYIKMIIWMRNDNQQIKFMPFHILMDYILQQNKRLQRHVFIKIENHLMNDTTIYEEEIKTLEKIYRRFYQEISNNKDKLNDMILDMIIQKAMEKYKKLYKPYKGQQYNKYLNQQQINYQLSKELRFVHIVDEKERIILEQTKQKYPINKLIQMKTLNLFSLFLQDIFKYCLINWDTITNIIIICYYFKNYALLGMILPIIMFVWGLIDVNSKIFWIISYSLFFFIILMIFVDSMYGVAYSKSDISEQYIPWYYILDNSNTFGLFYEIFTIWLITFQVFLQKSFGIFDFPFTQIENIFQGYIRLKLNNFNKDMVEEIVQESEVQRSRSKSEIGRIENPEIERIELENQIQGRSYLEKSGMEIEPESIMLQIGNDLERKIAANPDILSDKYQPGFFEKIMSYEYARPGKDLYLYIAFIQVILFLYMLFFFNFMQGKQQSIEGYLKYNQVPAQLVLALVFQMLFMMVERYIELRGDQKYINAKEKTVYKNKYKTQFIQKYILQVVILTLVFFFVYCYLLKLWSKTTENYEEYNTSIVIYFILFCAYFYLSSLQLRYGYRELKVRNQFLYVYNSISYYFALILYSIPFLYDLKVLMDWTCLNTSLDIYQWFILEDIQRQMFFTKISSQKVMKRQLGLQISKISKLIYFFFVIFILICILGPLILFSALNPATQNNPIVSGTFEINLVNVDTNLNVQFYESSQLFGLDLPIMRDYDEIKALIKQEKITKQQIDNSGGIQRFQLSDFSNSKWQVSLPLYNQLIRELNQTISYKENYTFMFNVTFTFRRLFQFILPTVYKFSYPYNPLINGENGTVGLGIDQLKYIRHAALDCDSSGIMLPNFYIDVIRIYQSSATKYNTTPIYGKDIHTAQNVFLNINCSYGSTNPLQGVSNWEFQYAGNNSLFSNSSNITLADGLLYFVICDNFSALTYGLSVITIYTTIILLIAKWIRSTFSSQVFMLEFTKMVQPDDLLSICQAVSVARQLQNYRKENLLYFELIDILRSPELVKAMTGAWSEKFDSQQNNQNQIDVKGLQNQKQTNEESKLKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEJ4</accession>
    <name>A0A8S1MEJ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0590079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEJ4"/>
    <dbReference type="OMA" id="ERMNRFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="296" mass="34708" checksum="3CEA2265788AC1BE" modified="2022-10-12" version="1">MGSCNSCNKYEQGLSNQEQQEETNSTQQVKIIKQSRDKPLKNDYEDYPIADESCLENSSQLPIFIQQKEIRDNVVQERMNRFMKDIEEEIIYIITQIIRRITKQNQLWSMSIKDQQKDSNDIKSDHLHQSISFQNSQNFETLRNLPCKTYLPKQYSQFAPKRDSETNTQNSQISLTINSLRTKTATNKNKFISIPQEDEKSSQASFVSLNESQQNFVSQEIQFSDNQPEQFSSNASKKRSSKSILKNSQRFLCLQRNLSLKSTYTNFKQKKVSFSQDTKYFTTRALRRNPTTLFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MF01</accession>
    <name>A0A8S1MF01_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0590154</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MF01"/>
    <dbReference type="OMA" id="GYSENCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21964">
        <property type="entry name" value="BREAST CANCER METASTASIS-SUPPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22586">
        <property type="entry name" value="ANCHR-like_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="476"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="512"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="599"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="805"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="947"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8078079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="975" mass="114946" checksum="9C69B430E0BCED75" modified="2022-10-12" version="1">MSDQEYGEDYENEEQHNEEENEDKNENLEQSNKNESQANNNEDENNEQQQNDNDGDDENQDNNEQDQQQDDQQNEEENQDNEGEKEEDQQEEKMEKLQQQQEEQEQEVEESQFEQSMQSEKNVNLESIDAVSVKISKEEKGTQKKGTKKATKTSSQQEMVNQLMKKHKEKQQKMQKREDEIYIQEKIEKKQPKPQIKPKVITKQKLTFDKQTKAQLEDPQLNNQQKAAILTKDLGKKLDVQVVVKEVPKKMEVKKPGKGSEAKPKKNLFDVRIGAGIKSKLDVEADDKSQRDKEKERNAFLEQKKREKEQAIKFQQEQDELLKIKQNNIRKAQEKALERDNKVLQVNTEKQQEIKQVYEAKRKELIKEKEEDNKDQIKKDLDILRALRKENTLLNDEVKTQEMKIKELDKLIEQESRKENVIQINGEYQSLRKIRLKQGLDDLKTELVSGAQSQILGQGNISDPDIALTVANQIAKNDLKLNDAYALLDEDKDGVLTIKEIQNNICRLNLNIKEQEIQEFIKMLDNNSDGVLTEQEFITFLTPGLENQKQYIQIMGDIKDILNPIILEERRLHLKYLQKYLQKEIEDENKKEREQKPKYDRLIKKLKKTEKLVESLKGTTSDKGKVKQQCDLLRQELRSIQDKRNKVIAELDDKKLMIKENIDQLMKDLGDRCQEVLQMKEKLNQVQTQSQRLQLKLLSIEQMKNKYKKELKVKNEIEIQGGQKQNVLQQLRLKEEKIQQQEDVKNLQYMHYVIIVQKCTRGFLMKKRLTKKLSGLKGCAKALVTFIEKKHRHQKMQGINLLEEFVKQQKKIEIARKEQELLRQQEQQKKKLKDKKANKVERFIAQQFVSTKVEDKLNNIDQTLSIIPKIILIQKIFRKNKRGIPLTNQAINVSFGIGYSENCQICKTDLAEVICKQCEKPIQCDSCFRLSHKNYKRRNHEFYLLIKNKFRDANEQLENRNIEKQNEMDEVLSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MFQ0</accession>
    <name>A0A8S1MFQ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0580120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MFQ0"/>
    <dbReference type="OMA" id="QRIIYKN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="32519" checksum="3B04FA5CBB28F874" modified="2022-10-12" version="1">MDNYSGANEEWKLSQKIVELSLNENDVVLQNRHRRIPQKDERINELLKKIPSEIRQMYDCLGPIYEINPKYQIIYQNDGSIYFGQGVGGRKIGTGIQIWPEQGNILDGNWENDELEGQCRMVYSNKDIFECLFKQGRANGFGVFQSQKKIVKGIWIDNNLSGEGQEFRKDGQRYYGQFQDGQKNGQGIIYFKDGCKYEGELRKNREDGMGTMIWSDCSYYDGEFRLGVIKGRGVYSSGNGYSLMGYFQEEVQKDKKQKIQRIIYKHESGSKLIIEKIRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MG07</accession>
    <name>A0A8S1MG07_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0580180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MG07"/>
    <dbReference type="OMA" id="NVEQHEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="223" mass="26505" checksum="91D3FF35FE52DD53" modified="2022-10-12" version="1">MKIKKQKALRSKYKSINSSERALIINYIEEYKYSTAHVSMITGHNASTIKAIYQVYKKQGRINKKVKRDKILNLKFQFMLFVYDDPKGNLTKINVEQHEYQKQIVEDQHMNQSNSQEQAIKRILECNQIKIMNLLDNANAIDTFKQEMKIIQSQELPSQQFSSLQLIPCFNIKENNQTKFLMKYNLKFISNLFSQKQDDTKEDEESKLSVEKKLNEQYRLMRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MI25</accession>
    <name>A0A8S1MI25_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0590165</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MI25"/>
    <dbReference type="OMA" id="DREQMAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035766877" description="GOLD domain-containing protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="3">
        <location>
            <begin position="18"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8078101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="196" mass="22747" checksum="C6CC1F7DD8741700" modified="2022-10-12" version="1">MYISLLLIGIVKVMADNFGFIVQSGEQQCLQDQLLKGQMMALQIIANSTEFEFTVEQGQKDPQQIESRKNEILIKYTLTMWSNEYLKYCFRVFGSKNAKFDITYKKGDETVDREQMANKEDLDLIDGYLKQLDRVYAQMKDLNRFQKGSQENNQGLGETIGLQMTTFTMLTLLTLCISTYVMVKQIKVTIRKQKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLB8</accession>
    <name>A0A8S1MLB8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0580217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLB8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="21732" checksum="4CE6E61E7E37770A" modified="2022-10-12" version="1">MKEIQHFLVKNQKCILLIMNLFSLEIVKKVNYQRIQKRKSFIQEMRHHLDIKIIISNIWYHLLNKLCQQIQKRNSVKKVLFMILFLNLDKQRKLKYFVKKKIKTNVSSVIKSQIQLCRQWQLCIITSITKGKFRFFFQKIKLEFFLLFNELFNFEINYISFCKIILLMISIISKYLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLH6</accession>
    <name>A0A8S1MLH6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sidoreflexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0650150</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sideroflexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLH6"/>
    <dbReference type="OMA" id="LQRYVPF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004686">
        <property type="entry name" value="Mtc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153">
        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153:SF6">
        <property type="entry name" value="SIDEROFLEXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="235"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8081298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="36402" checksum="C0EF05F571EB586A" modified="2022-10-12" version="1">MKRYTNKEDSAEDLTTYWGRLKHFQNIISPANIFYSSQQLNEYGKILENVDKDPSIRQQYTDQQLWKMRYVVDSNIHPQLKEPVNILFRTSTFVPVNVPLAFGLAVLPPTPINQLLAQSANQTYNFMFNYCNRNASNVFSNEMLAFSYGGAVSSAVVGSLGTSWLFKKLNAPALLIRACPLFGVLIANTFNLFFARYPDFQKGIQVFDDETQEPLPGLSKEAAKIAFFRTLVTRYILPLPMFIPPIVIYFMKQAKCYPQGRGIGLALDLSLSGFFLYCGLSVGIGIFPQYLKVNPTDLESQYQNLTNSKGNKIKTIVFNKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPA7</accession>
    <name>A0A8S1MPA7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0630173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPA7"/>
    <dbReference type="OMA" id="GIQPWIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000253">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF201">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035731462" evidence="1">
        <location>
            <begin position="18"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="473" mass="53601" checksum="7EAC63D6987995D9" modified="2022-10-12" version="1">MILSLLYFIGYVAGAPAEDLVDGSTWAKFKIPYAGKMYSGYLPIDDAGKKQFHYFAFPAFSLAGPLQATFPLVLWLNGGPGCSSLYGAMVENGPFTVELGTNNFKQNLFTWLNFANMFYLESPAGVGFSFGNTTTDDKSTAKDNLKAVLEFFKKFPEYKSIDFYIAGESWAGIYIPTLANEIIDYNAKAAVGDRIRLKGLMIGNGCTDPTECTDLGFNFPVHFYKFLHNHGFISEKLNHKIENMTSYCHMKSYPECIEIYGEVMEQINGDDDYYFNPYNVYGKCYQLPFYNEKGERVKEKRFKLHPMKEGVVGQINECSESEALFLYLNNAAFRKALHIRDDAGYWNDCSNIDYKKDPGATYHLYPKLLKNGIRILKFSGDVDSIVPITGTLYWIDKLQKELNLPTIQEWRPWYKPGEKGSEQQNAGNVWEIDGLTFVSVRNAGHMVPMDQPEAASIMASHFIFEMPLPSDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQ55</accession>
    <name>A0A8S1MQ55_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0690156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQ55"/>
    <dbReference type="OMA" id="HICEYFG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="324" mass="38951" checksum="7463386D35F87B2B" modified="2022-10-12" version="1">MNQSLFLNNFINKQQICEYFGNVLLATDYSGQIVLILCNGEIITFQLIQQKQWLKTSQLRVIPQTEIIQLNDYNLQLNGNGRKFFLLQVDERYEFKVSIWKRGHKANWIFQQSLILYSHSDFLSITLNGNIVMVNHLKMIRLWEFNHTQTKLTRVQSLSLESNLACFNENNSVIAAKLQYRIVLLKRVNQMWIKYQNIKTEQVVFMQFFKNRLILMNKYLYMYKMDEQFQFQLEKKIQSNFFQNEPQFCLFMGSIIIAQLPSLELECHKYENNVWSQDEVYFTGKFEANQILQSKDYKKLFIYRKRYGGMVFEKKKEINEKIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MRW4</accession>
    <name>A0A8S1MRW4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0620245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MRW4"/>
    <dbReference type="OMA" id="FNETIDW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08774">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="1">
        <location>
            <begin position="5"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="243" mass="28755" checksum="7D020526F2A3B67C" modified="2022-10-12" version="1">MSDKRDEYTYMARLAEQTERWEDMVENMKKVAEITKDLNNEERNLLSIAYKNTVGQRRTAWRAITAYEIKEKQKIVKYQEVLQYYKARVEKELDYYCNEVLQLIDHTLLKKTTNTEARVFYHKMKGDYNRYISEYSQQKAQEKAIQQAQQAYQTALELIDKDKFPKTNLLRLGVTLNYSVFCYEIMSDVAKACELAKTVKDEAIKELDDMEEDQYKDVTTILQLINDNLTNWQAELPDDGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTH1</accession>
    <name>A0A8S1MTH1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0690069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTH1"/>
    <dbReference type="OMA" id="KCWFNVT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06463">
        <property type="entry name" value="p23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="2">
        <location>
            <begin position="116"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="305" mass="36402" checksum="DFB6294E78126C9B" modified="2022-10-12" version="1">MLFLTFLISVSMASQDLLKKCYLEQFTIGDPEVKIQIYFEDHIVKNHKIEYECLEFIISRGYYKVALSLYENYFLLNHIDITDRIVQFLKNDKYLNQREMQTLFKLAMAKSNQVQVVQPVVQWAQSKNATFINIKFSHRQDAPACLNAKLEVVEIKNDSLLIEAFGIVSHIPFKYRYAIKLYKPIDPTSSYEKVESVGTMYVNLTKIEPVLWLRLTEEDYKTPIWWDLKDNFRKDMEEFAQMLEKESERKERNADKQAKKNQKKRDQEKQKQTSQKAQEAKRQLEYEHNQCYKPGKCEIGWYQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWU4</accession>
    <name>A0A8S1MWU4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Choline transporter-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0660244</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Choline transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWU4"/>
    <dbReference type="OMA" id="YCFVIAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF14">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8081936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="564" mass="65159" checksum="8B1A248F85CAFD30" modified="2022-10-12" version="1">MQEKQEQRKSTDLICFWCFSLFQAFFFCISIYALLNGNPNKLGVPYDPDHSSCGFDLEQYKYIYFTTPTNNFLYRTVCLSECPNTQQITKLQCSPNSIVKSCQINPSVTNISESILAYPSEAYNSQICLPKNPDYLNEIKEFIVPTVNQKIGSGLRDLKWIILAISLISVIISVIVLLFIEKYSTHIFWGYFVIIVLSLFSLCLFSAQQYYSSQEQLKLDQQLETYQIITFANSLQISQKLYQIVMIISIIAVILSIIYAIYLKSQTILLNLFIKVGGDFIKSNIACIIAPVVGSVIIMIYSFIWIEQQLYLLSNLKIQDNKYPYFQIDFTQDIQVLMIMNFLIFIWTLAFIIGLIEYVTSGMVCEWYYQQGNRMNFEKKNILSDLIQYHIGSIALGSMLITIFKFIKQILNFTKICQGFYNRIILAISKHNYVIMHIQPNHFLDCGIIVRELIKKEIDTYNKIGELGQTFLGISRLSMALCCQSICMLVIQDHPYSIILSLYCFVIAYSITSLFISLYGQSSEAIYMLYLYSQNHLSQEDNPQCTITLQLVFNQINDNINQMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N015</accession>
    <name>A0A8S1N015_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0750197</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8086038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N015"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8086038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="53" mass="6200" checksum="018588BF232A060E" modified="2022-10-12" version="1">MSLNSSKLVDSRSKTKKYSNSIYIKKSLFTNPIKLSEQSEKFFKTWVLQLVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2A4</accession>
    <name>A0A8S1N2A4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2A4"/>
    <dbReference type="OMA" id="RACIFKG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="13231" checksum="AD7C06314F2B0F90" modified="2022-10-12" version="1">MISNEKGLSLVFYLIIIMLIFGMPLAIYNYFKQYRIKQKEVQRLFQNWQQQLENSQDKIFYYRQSQYEFESRNPDIADQDFWDALDVLRERACIFKGQDYWLSQQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2C6</accession>
    <name>A0A8S1N2C6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710085</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0710086</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2C6"/>
    <dbReference type="OMA" id="KSINQHR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="184" mass="21791" checksum="F37A3A4A38F50AC8" modified="2022-10-12" version="1">MNKLEQKAVSSLLKLAREEDTITQRDWEPQSPKQVDFISPQPKSLNQHRKNVKVIQNHKARQKSSIEINQNDDQQVLGFSQKENSLQQLIEVYMKQIRHTQRQMESIHQQKFDLKSGWVNFSSIILESKEKLPKKHKRSSLELQVKENNQQKVQQQQSFKKTPLRKVLNNDSFFLPKLKSTYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2Q0</accession>
    <name>A0A8S1N2Q0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0770098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2Q0"/>
    <dbReference type="OMA" id="TICPQIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="60549" checksum="3D2C0F97A9B3A06E" modified="2022-10-12" version="1">MQVTGEITQQEFNYLVEFPRQNLNLGYDQLDQNLKQVLDRISVSGFFENKSLDIYIYVSTGQGELYNLTLRNSIQILLNYQYEIKKKYQIEGTIVSDSPYVYYSYKNYLTMKSEFNQLNEQILSNTIAKSQQEQSQEKLIFIIAVGIALIQFCLSFNYFLQIQRLINKFYGVIQNMDTDYTYQEINRLKFISGRLNKNTNPLFRFQINIEQREKEFQYKSYIMNIKKKLLHRPYYLKQYFVYYLYIIFVLVLMIGNALLTYEECGEYLSKYPETAQFFKAISDVGTDIPTMYAQRDILYNIELIAPFLNDTEKSRVLLEIKESLNRTTKFITLDFNMDNLIISTEFKDYYNQIQKENLCNFLPNYISQKSSTICPQIMDQNLERGLLGLLIYISNFINTDMAINHFTKKLQQSYLELEGAFLVSYIIKDINTSFHYDLVSQTQFYINKISVHNLVILIFLCILIVLTLTKIKNKLIYKLYLAQRLPYLMPIKTIILNDSFERNLRQIMHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N417</accession>
    <name>A0A8S1N417_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">B9 domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0720087</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N417"/>
    <dbReference type="OMA" id="FELECPT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036038">
        <property type="term" value="C:MKS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010796">
        <property type="entry name" value="C2_B9-type_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12968">
        <property type="entry name" value="B9 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12968:SF2">
        <property type="entry name" value="B9 DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07162">
        <property type="entry name" value="B9-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51381">
        <property type="entry name" value="C2_B9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8084456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="235" mass="26544" checksum="57F749E98A9C15B5" modified="2022-10-12" version="1">MSMPYQQTGNQLDYQEVKQTKADLKAEVHLIGQIVGGLDFQTDDGLFCELAIDCGDGWDLLQPGNNKGIQTQTSYANPGQLFSWGHPFDLHFSVSNIIGWPKALLKVWRLDSSNKIDACSYGTAIFPRSAGYHQITCETWTPTGENLDLEIRKPNEDLATNTDQFSKFDRSLDWRFQALSFYMENPPRLTTLAPLTTDKGDFARRKILNSISNGRVIIEVEVILKNFKRLSFSGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NB56</accession>
    <name>A0A8S1NB56_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0830150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NB56"/>
    <dbReference type="OMA" id="LEWKARQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="334" mass="39897" checksum="1B440233B5D89E4D" modified="2022-10-12" version="1">MQKYIDNQNKLIKMLYLLLIPALAYVQEQYLMEIYNQMNGIKESISQLEYYTALEKLEWKSRQDKEFEEEGMTIYLENFADVQRLLKQRLTSEKKEKDGLELFLHETYFKVINLLKRLIIEKDKLIETDTRELAKIKHQARDDVQKRFDEFNFLLQKIKEVFDSTNIQDIFKTLELLESKIMIGLFKSNPSQELDFFIKGVDRLFKLSNHSNSRNEALQLLSLMHIVIQETLIGMMNEQLESSFDLLDKELFYTQSKNTHQQAKESLINLLNKFEVTGAKFWNEQLQINGNEDHQIQMFQSFLDQAMLMSNRSIQYHQQLSDTLRAMLERFLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NC95</accession>
    <name>A0A8S1NC95_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cation-transporting P-type ATPase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0780056</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="PPQRRMA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="4">
        <location>
            <begin position="49"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8087153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="215" mass="24523" checksum="6FC7447BC1F31961" modified="2022-10-12" version="1">MADEDPMTQQEQLKDDGAEPYGKFRIAKQTLCSIAYMCQERVFAEEVDKLEELGEKLIEEGLCTDFKQGLTMQDQAERERAFGHNRKPKIEPKGYCELWLGALNDFTMKVLCVAAVISIIVDVTTADESYRKIAWIEGFAILVAVIISTNANAVNDYQKERQFQKLNEVADERKRVTVVRNGKXFAVFLNQFVNFLLSICLKLILQSSQRQSTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCR5</accession>
    <name>A0A8S1NCR5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0860018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCR5"/>
    <dbReference type="OMA" id="NDPQHPM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="267" mass="31654" checksum="54BB942531AC0338" modified="2022-10-12" version="1">MSGSAYHNQKPIYNNPACEQQKQVQILKLNQKQQQEEQLIRYYIFENLQYFINLIYSLKAVNEHFQNDPQHPMKIIARNKIVLQSNLSNSIQYDQQYQYKSDSKLYQVLQQEKLNAKQLLEQLSPELKEEFQSYIDAYKTYSLNQNIQMCSQNLEQTNLQSKFDQKVLQSVINSISSVDQTTQQFKEQKIYNRPKMQDVILKAQNYYEKTKQQLDINFPNPINVEQESNQSLNSQAPKLKLKQKQKNSIGIKKKIYKDSINQHNKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGE4</accession>
    <name>A0A8S1NGE4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0800084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGE4"/>
    <dbReference type="OMA" id="IPKSPMY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="166" mass="19345" checksum="A0DE1A610FDAD042" modified="2022-10-12" version="1">MSEELVRLQTIEFKQEIEKRYAKSRHNHNYVFEREGNDFSDDDSLDSLKNIGLYNLCKPIRNKSMETERTMPKTSKTLLIRLLKPKKQPSVNENRSNNNSLYKSRSQSKNGTTRLRTEGSVLRESKNVTLISPKFPKSPLYIQVDNLIKSAKNKNLYKLNNYSMAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJM7</accession>
    <name>A0A8S1NJM7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0860159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJM7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="61868" checksum="4A34D88F28935DCD" modified="2022-10-12" version="1">MLYFLQSVDQFGVQQKLQIPPINPTQKSALGGLVTLTLYGISLGYFLFQFVDWQQNNKLPKITSLHQQINIDQTLKFQGVFAEISYFQELSNQIDPFDPQNMILQPQLIITTSYFEETTINATFQYRKTQDGKTVNTLFIEDIEIKNSPIGSPDHKYINYQLSFGYCNPQLLQNGQKCAGQNLINQFFLQDNYYQMLIYLEQFDPKSKQIVKIPRFFVFELIKNSMFQNQFMLKAGDLIVDDGFLFPNSNKYNYFSDLSIITTQFDQEHAKKVYGEELISIFYFNLDQIKIVNMVEYPKISEILADTGSIISWILSISFIVSKYNENLSIEKTQREIITMYYNDFIDFQIQKNWLGKIQRVNCKGKEYDLQKSQEILNRLDQIAIEKMNYLNLQNEVAKLQLILQEHIGLQQIKKYLESKFSLESLFDKLGIPEKISQQQEINQIHAQQELQQEVDQQLNIEGEIDSKFNKDIVEQEVEQRMGLLDMKTHENLQFREQNSNLQIQESLQQNNNYLTVINVDKTDEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKV3</accession>
    <name>A0A8S1NKV3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0910105</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the amino acid/polyamine transporter 2 family. SLC38A9 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKV3"/>
    <dbReference type="OMA" id="ICLFFEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF244">
        <property type="entry name" value="NEUTRAL AMINO ACID TRANSPORTER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="16">
        <location>
            <begin position="8"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8092742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="409" mass="46314" checksum="B4E35C0FA112FB6B" modified="2022-10-12" version="1">MNKSSFIPIFTLSNGMIGSAPIVLPVLYNQYGFLNGVVFSFIICLTSCITCLIVKKYHLKKEVDLQFLINRILGPKFQTSFNFITIIFYIACAQIQVIISCSLIYSVISLFVNSAPQNVFTFSQFSYQILGMIYILILLPTFFIKDITPLLKLPTYASLATLLAVIFIIFQGSQSPTISLWKSNDIAVLPSVFQLAFLGHSLVVPLLSKSESKHKSRDIIIAYILTFTIYQVVGLFGSYALFERKPQEGLSGFTILDYFPQSQLFVIILQILLFIQISLLLPIIQYVARLRFFAFFNAKNPKPYMLIIFSFVFAFICLFFEMMNLNLENLISYTGAIIGTIIGYILPIACHIKMKYFNNKKVQELNLIESSQELQTKKIGKLEILLDCLGCGIILSIGLSVLVLRFIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NLY6</accession>
    <name>A0A8S1NLY6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0920153</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0920155</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NLY6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8093166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="181" mass="20465" checksum="58AB884A113D8D19" modified="2022-10-12" version="1">MKTLTLLFSLCIKVQKNAFYIIFLARYVGENRAFPSKGQQIGQLILINLAIKLVNLRNLRCLKKQILLLQPQYKQSANKDQAKPEPKSDQGKKILKLIKGKQILNLIKANQILNLIQGKQILNLIKAKLSLNLIKVILLIRVASPKAEKGKDENFPDQGMTDPKADQGKAKRGQKQNTTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPT4</accession>
    <name>A0A8S1NPT4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0950076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPT4"/>
    <dbReference type="OMA" id="KSFMCEL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013887">
        <property type="entry name" value="UPF0592"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35397:SF1">
        <property type="entry name" value="ARMADILLO-LIKE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35397">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08578">
        <property type="entry name" value="DUF1765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="989" mass="116339" checksum="B20092B57A54B5DD" modified="2022-10-12" version="1">MTEQQMRESNMKISVMDTQVIGSATINLFEAAVGPFHFQLPIRGQNMGSVSFDLKMNQIVQATLQSKYIIWELNQSLSDVRYNYNLRLVTSQFSFISEHSTTFVNPNYRKQNEKQKTIMSSSRKSILDQDAQRDCLISLQKIPSIINNSAGINHSLDSIITATPTNKGQPSASQPYHRIEWDHAEDELTLIVELPISEFQGSAIQLCLWSISSNKNSLGNSKSPHKTTIKKTQNKHDSEYMDLEIQDHHLVAETYINLQHLENNIVADLGAKFFVINSQQAKSIWYHGVEVGKIDYEINVKLPNYLQQSSFGVQTEKGIVSTLSVVDNVENISVTEIKMIQIEFQKLSSCIFKIEHKTLNTDDKLKIRQDMDQQLNKLAQLLGQSHGSNIKTFQYKSEDDLMKAQHLLIQLSIHLVDYSKTLASFNSYFECLNQVLSRGELMLQQLGFFKALSKKQSEFKTEVGLDYQSFLVNTLRFTLQKLNQKDPSQQARQLYIKFLVISYFRIPEFRAKFLELITKVDDPQLLELRGTEFIQEDDPTNIDKSTKTNIQIFDWQNYFHTYLSDKSKGIQNQSVLNQILDDESWKEHIRHRSINFCFFVEEWAKYVRNILQVKILPWQDIPGYRILVKSFMCELKQQESVPDAMKMALKSLLQNQNLLGIVVSLLFNKTNLYNSEQVIETFEILDICFSTLTTMPAQFDYPFFLKGIKQIIMDSEHAINIAKCVWLIYNIYPLFPIDFKKDICEFLFEKAVFKLFLHWSKTVRLVFHYFLIYRVSHQHKNLKIGGLDEEQIIQQYTLINRPKKNQSYFENRQPQQQLISDYIYIKYLRFLSKLEQAKANENQREYQQDHQTYYQKMVHKKLRKEQDLKRQQQDEPKMMEQLKLERMISEQDESQQKQNQLKPVIVPRPVMYKKLSYKHEVEINEMQQQYLKLSIREFEEQVKNYNNWRQQNLKKMIGKYNEEDKHTIHLTFEVPKVDVLRRIDEKEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUE9</accession>
    <name>A0A8S1NUE9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0970148</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8095019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NUE9"/>
    <dbReference type="OMA" id="RVNTICF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8095019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="489" mass="57574" checksum="ABBC3C38C57580D0" modified="2022-10-12" version="1">MQNSQLEIIEYIKSLLCPHHNLPIKYINYSTNVKIGDRLFCSKCAEKGLDGYFEIEAAIHNINQPKISQPIKNACLQLSQYLDGIKTKVDSLTNQQIYFLKNTQNRQGITKFQEETQNYYLYNPNEQNTEKIIKQGKDAIQNSIRYLERFQMQMLSLLTHQLNQFKNFKEIHKQEYQQNIFVETSIQDYEFFIYNSKFLEVFTTQDNQINSIQTIESPKTKITCITKAQQKLMFFLGRQDGIIEVYEQLTNGKYISSHQIQAHSYNPSQNYNGVTCIITNQDNSEIISGGQDTLIKQWKFRKNKWNLISQLSEHTGVINSLCYSLDYNILGSTSQDYSFSLFKSSQDVFQKSIQEIKQSRVNTICFVTSTQFFISLNQDRNIYFYQIINPLQLQYQIEQLYNISLNEINTDIHIYVQPRFYQQQNLLIFQSNSQTYLISDKDSNKFKIILIEQQNSLKGNIFRLMRFGQLAVQVYQKNLKYFLSLTQFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYK4</accession>
    <name>A0A8S1NYK4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pentapeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0900224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYK4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="31658" checksum="66C302662AAB15C3" modified="2022-10-12" version="1">MMKKIWDELSQSIKQVYDWIEKENQSYFNIINENTNLAESSYTDLEKLLIKAFVEKPKEGIRQIQQSIEKEEVYQMKEDLFEILTYIQDIDESLYQKILEILRREKVLDVLKFLSNKNNQNFYESLSNKQQNIKDVKKQIMKITNVLRNIQDHKFNKYDYSQETNEKERLNLINRMKNNKGIIDFIRFLVLLTSIDGKFIQSGSNGLNLCVGMKVDIRNKSFENIRIKNTSLIGGNFVRCNLSGSEFENVDISGVNFMVLNYSIANGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1L7</accession>
    <name>A0A8S1P1L7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1020080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1L7"/>
    <dbReference type="OMA" id="PHFSQIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="317" mass="37292" checksum="D38002BDA4B9A099" modified="2022-10-12" version="1">MITKIKQFFNENTSMSAINKLTLALSDLKLIVNDTNFMSQLQISNRLKHFFTNDQTKILFLNQLLQVVGDHLQQKEKFYVKLKMLLLVHIIISSQVARADLSKIIINTKLTINIKADDGTDNLIGQLCQSYYCYIYKLASQTTLINEDFGKPQDDLMIYFTLSNQCYIGMNMQRLIETINNEQVPNDIIAHLVKFLYFDIQDIYIFILKDVKYLIEKNPNLIINKQQLLELYKECQSLQTRMVTFYKFNRIFPHFSQIMPPHSIQIKNSVLNSVLEDKQIQPFQKSNQIEIREPLSVKNSDKKFYDYTRPLSPKEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P2E9</accession>
    <name>A0A8S1P2E9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0990165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P2E9"/>
    <dbReference type="OMA" id="QYIPRVD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="101"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="183" mass="21741" checksum="FBF2AA46A1CF46E0" modified="2022-10-12" version="1">MNFEEQMNLINGNPLLQTFFLNNLLYNPQIFQYMISQMQYIPRVDLREENTKTYDDQNSDRKIQKQEKLTQNSKQLLVGIDTEQPHKGNSHSEEEESSSRVNGHWSKQEHQLYLQFVNDHEDILRSKYDKKSKKIFKLMSQCILTRTATQCRSHHQKFNPLQKSKRKGKGFVRPIPSVIIQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8D9</accession>
    <name>A0A8S1P8D9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1090065</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8D9"/>
    <dbReference type="OMA" id="YYNSIYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="26"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="394"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8099275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="60969" checksum="129266B9B04CDC5A" modified="2022-10-12" version="1">MGVCLSKKLKMNKQQSPVNKNLEKTFQKTKYYQEKYMKEVFDSEKQCEIKMVNVKTSQSTDAALNNSFLRRKALSKSRIGLSTNRKGAIPILNTKQVSFKDSFKQKYHKVIGLLLSNSNLSVVQNNQNQQLAICQQLRKNDEKDQSFIKWLKEKNIEHDNIVGVQEIQVDDQDYYIIYEYWVDQQIQNKTELEKAILINQMIESIEYLHDNDMRHSALTLNSFKLINNKPFGVLKLIELNKVHELSELSIESWKYLSPESLSALGNRSKARDVWALGIIALELLMNEHPFIKNVKEISIRKIMMKINSLNNQQIEQMLNNLNKDLKQLISGMICIDPQFRYTINECKTNIWLNNQINDYISSLKQPLYNFDKHKNNSINNIMARYYMVRILDQKQFNCLFILFQQLDLNKNGIITPTEIKNYYNSIYLPSSCKNDLINCLYSLPKDMEIHVKEFIGLTCDISQSKLKQYYTINNLGDLKLNCYLNQLNIPKRVINLIISDFNISELEQIITLDLFIQYII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P9T0</accession>
    <name>A0A8S1P9T0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1110029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8099926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P9T0"/>
    <dbReference type="OMA" id="QRSHHTH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="504" mass="58996" checksum="17749BBB71E7C526" modified="2022-10-12" version="1">MKENQKSDIAPQDKQWEWIPCQYDVKRISKDTETTWNLSPSVQLKIPSDQGQIDPVIDLKIEQSRNFIYTNKNGLGWNWHKSWNLPDIGVELEITDPTTDEKLAPPGKIYAKIQIVKPTVSPMKTFHLLEVGVKGNQKMDLENNKCIFSGLKFNTTSYNHDHQKFHIIITLYLCQSKFEFPQILISRISPPIFVDSRKSARDIEQQNKQKLQSYFDPFPITTLEKQFTTTKPQNTEVITNTIEGLNNYFSAPNIRHKVKHPVFLCIKFSSCIKLYLNTNVFKFQDSKSLVLTIQQVLYKSTNNNVNNKLDQKYIILCVDSSNTISKFQQHLKKLQEFLEPFNNDDIEVVIDPIELPKNFTQITDLADIKQAYIDVYDKLLQLKKNEQQHDDNQEQEEQPRKKKKPKEQEVVQNNQEKIIRKVIQIQGPNISQQRSHHTHVIQSAEMLIEQQHQYLDKEHVKEEHIIQEEKPHQFGLPDLIIQQTAINLNKFLLRALLPQQQQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PDA6</accession>
    <name>A0A8S1PDA6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1140138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8101156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PDA6"/>
    <dbReference type="OMA" id="PRCVNIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="123" mass="14673" checksum="84B206EC794B76A1" modified="2022-10-12" version="1">MDCCYIICSCCQINVGFLKCCTCHKDFEFYMQNYLPITCPRCVNIRLDTYYQSDQDVQLHRNCNQNIKRMFHYCKCCDGYFCQCVQQISIKLQVNKNEKINCFKSSSYPTSKDRRVIRKSQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHI3</accession>
    <name>A0A8S1PHI3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">guanylate cyclase</fullName>
            <ecNumber evidence="4">4.6.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Guanylyl cyclase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPRIM_AZ9-3.1.T1180138</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">In the C-terminal section; belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the N-terminal section; belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="TQNTQEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000026">
        <property type="entry name" value="Guanylyl cyclase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF12">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1126"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1158"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1206"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1258"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1288"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1405"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1431"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1465"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1515"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1538"/>
            <end position="1558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="2020"/>
            <end position="2039"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="2051"/>
            <end position="2069"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="2084"/>
            <end position="2111"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="18">
        <location>
            <begin position="1615"/>
            <end position="1750"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="18">
        <location>
            <begin position="2198"/>
            <end position="2329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8102494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2395" mass="283943" checksum="5FABEF47BDFB55EA" modified="2022-10-12" version="1">MKQLFLNYRHNNEMRKYTYQIQFRNGETVSYDGFLENENKIQRINYTILFPWRVIKENIFYFLLLILQAFSHLPLYFVMIPLILQQWFVDLHNCLKIRKMDKMIKQQEAKIIKQWDECIVNKEIISKIKERLNQQDQSESNQRLKSKSQVDKNSQNKKIEYVQSQKKGISRSILSNDSIPFQSIQYKQQQSNKNIKASENIKKYNQMSPLIYKDMALKLQQIQIMETDLLIIQFKMKVPSIFPKDLVQTTTKIPLNEKLKRMEKDKMNLIKYQNAEEIKIGDVILLQRNEIAPCDIIVLHCNDENFCVQQQLCDYSQYTVRKPVVQNRSDSQTLAQFKKSLTGNIVFHEQNSQIKGFFQLKKDPQSKTLGYENFIFCQEKLLATPWILGIVVKVGNSCFCYTKLQGQLRFDNNQYFGFYLLICIIMIIQIIVFNQNEYLYELRSITQIILDNCIYLILMIPHFFKIYYNICSLFQLQIIGTIDQKQIMSCHIDKYLEKQYLTISVEAFIEQSFKLKRIIYNSQYCDFEESKFLQFLETAEKGINKQDQDKINNQLNSQVLNYDMQSSSSEILFSQRECIINQIPKSFEGRCVHYYQQDYKSDLNLNQEQLILQEQIQHDKNIFENQEIGDHLTAQRQSNQQKSSKIYQSSKDQLKQDKYLIDENTLYKSMIQNNSNNDLNPLFLQLAMNHLAFSKLLITEKKEQKVKNKFLGLLDQKQVNLAKKMGYEFICVNNVQQQQHYIIQINKELYSFKLVNTKLHIQNQRLYMLFEMDDQSQEADYQFILFLREANVRKNESIEDKVLKHQSDSLHYIYYYYCFLSQNEANVYLSSAESNISENQLYTLMEQLLKLNIIFGLSYELKQDCQDFMKNIRKSDYQLFIYTQNQQISATSILYQSELLQSNDLIFHFNQQNEEDLRAYFKQCLEEFSNQFSHASINSSQFIKISNQSVQKSNETDHIEEKSRTKKVVVILNNSTCQQIMDNTYFKNHLKLLLNFTKVLFLYQATQDQLNIVQELWCISGKTINLIYENQSLLRCINGCQLQILQLQEFSLFRNQKFGEVNRKNFFKKLSLSLRQRLIYEKCYFNELQINSNTDVIIQGFKELDRLLFFQSPLLKILFRSLQQQTIYKTITFISYFTFTSIIYTWNTLDQQNYLVEIIFYFYIYQLFLFVIQHYQFLNDSKQLLYNKDQSLLLQKYSKHQSETEIQITIIIKNILQGILISCIFLYDIYSQEFYLYIFMSISISDWLYLIINFGFKFKILASCIFTILFYIYTLIDSLENKEWQNQISTQEVFSIILGVSFLLITNILSDYLQNYSLNNLPTSEDEFLSYLNYIHLISAPAQKKKTVTILQNKVNDLFDNIEQVDLSIQKLLLNEPNYQSRFGQWTQQIFHKAQTILQWKQKQIVQCLQLLFYHCTFLLIMYFYQDKNNYFLVDYLIVFGLQAIVFIVFTFIQLPTNKQHYLEYAKFIIATAATISILFTQNTQEHIGSALVMQYFITYKLSIKFHPIYDYRTYLGSALIIIIGYIIWYIIENESNVSFLIQILQFIIIFSASQYFIKSYYIQLEEDQAQNKLNFIEQNNKINDILGILLPKFIRDRLNETDQYNIHQNQGLVAIVFCDICNFDQMVIEEKDKIIPFLDDIFRTFDKYCQIYGVQKIETVGKTYLASAGLKACEQELTYLSQVDPVQRALNLAEQMMNYIRSKQWGTQGQQLIGKIGIHYGGAISGVIGYHKPQFSLIGDTVNTTSRVCSTGLEDTITLSEQAFDQIKNENIEFEIRNVEMKGLGIRPTYIFKCKMQNKDNNHSIQYEQDLQSKDARSNYVVRKNPELLKKNLKKRKTILTYIDTMNQKRESIQDNGTHNVFGIKPTDPAFYSSQDENLQESLQLIQKQSSEDKQKKQQSLQQPSTFKRLITMFQNRFQLENYEPEIDEMIDYEQLLNVILLKNEYTLEHNLIKETSFLQLLEISYYKKQMNQFISYDQYMEFVKIQSKSQTIYNLRLYALYYMIKQFCQIQFYNDYNIGLIVLQWLCCIMNLIQFLINRKPNFEKWKILIKILLFFQVILAGIVIVLEDNDELKNVHVYEIVFIQCIFCSIQILSFWVKVLFCFFTYFYSIIIIIIDGTQLISIFFFFVSSMYNLTLILFIEQQQVSCFNQKNIFKTQQQKESQLLQYLLPKHILNKFLDDKINNIGICDKLDKLTILFADIAGFTEYSSKVNPEDVLVMLKNLFVEFDRKCCELNVYKLYTIGDCYVAIGMIDYNNRNPHQEAKNIVDLAFEMIKIIAQVRKQINFDGLNMRIGIHTGSVFGGVMGTDIVRYDIYGPDVLIANKMESNGVKGFVHVSQETKAYLEQDYDDLFNFELHKTIDLKTIQRQIEGFLVHKLEQDHFPDEEDQFLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIU8</accession>
    <name>A0A8S1PIU8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1200005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="WSINNIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035847369" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="2853"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2395"/>
            <end position="2413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2451"/>
            <end position="2475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2503"/>
            <end position="2524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2536"/>
            <end position="2557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2591"/>
            <end position="2617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2655"/>
            <end position="2681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2701"/>
            <end position="2719"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2731"/>
            <end position="2749"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2761"/>
            <end position="2784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8103046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2853" mass="332112" checksum="1DDEFEE707632CA4" modified="2022-10-12" version="1">MKNFFIPFILGLNNLHFVLPSYIEGLNEQQQFQTFPFHSGMQNYNKFDAFSYGVWSKYLPLSTISQVGLIGMFDSHCFLQNSIIEKTNKEINLLYYDCVDQQTNTIQKKVQFLSSDNQMHNYKYEIDPLMYENYWYFFGLNIVLSEGIFGFYIFQGEILITSQQLNIQGPFKHQDLTIIIGGGLIVEESQILWIQEIQKLSYFPGKLTILHPTIQNLEENYDRYDYFQSFSKMYNPECITQTNSIENLPDFDIYKLNHNVFASENINCDEFQFETWIQISNFRQEDQQFIYQLLKISANFENSYAKNENLSPFQLFYEFSNSQIKLKVTTYSLIFPLVDYDFKKQSFLQQKEWSINNIKLWHFLYVSLSNNILQIQITFYEGLDQTLYETQFIVNQFHEVQFKLQYGNILQSSTNYLTGILKNMKFINSASQGDIFKGCHHSCKQCEGPTNKDCLSCSEESNRIYNSQYKVCVCPFDYIDDIECKSMENYNLLFDPEFEDYSTSNCPQGYFNKDEFCLKCPSFIKDTIMTCLECIQNPKSWQNDPYCQTNLYIDQQGSPAQYKIDNLKTYYIFDGIDIKPQQLGLYQDQDQDQDQDQDQFDSLYQDFQETNKNFLRFYSSKKVIESYSCDLENCEICVMLITKQICQKCSRISKLKNGICEKIIRGVIQQNNCLAPYYITSENLCNLCEIENCQFCFEYASNDLTKCTLYSDFQTFQKDEFLKIGCALCLDGFIFDFTLGKCIYKEPTLPNCQRSFINPLGQQLCTLSAIQDFGVAPEIANCQKYISNCKQCIQTPQSIVKCIICEDGYSSSLTTGHCRICQVEHAKLCIEGDYTKGDAWMQQIQSFLMLFLPNKYMYPKSANLLYITPLAIKCIDKYEMNQNFCRLYCDISCLSCKKEEQPYNGFTCNKCIQDYYMEPLRSIEKETCITCPLLCLVCEQRTTDEIQNINPSYEINDQNRKYTYKCIQKRQDQNIIVDPYQQIAKYCFNGICDNLIYYQIQNNCEDLYKIFDGWSSFSDDFNFDYFNHIGAQRFKLIIPIQDICILNEIATQSLSNELKKRVFSLFWVEMTLLGNHSPSTYQPIITIKNFNSISFKNLFFIIFQTLELLILNDDSPTNLILTDSKFIANNEEQIYLSIQTEQCQHFEIRNITLSDLNISNSVVFLIRFHNKNQDDSIKLLNFTIRNCNLTQTILFQFYNFPNDITIENFKIENCQFYNSTIFSFIQAINTQSMININNLIISKSFIQNSLLINGTNTHQFKLVQVSLNLNKLTNSKFLLFQNSLIIRQLNLRDSQIFQTFLFYKLLSNAQSNSLQFDQFDIQNNILTNTSLIFTEQSSTLNQVVIKLQNVNFLENSIPNAQNSTTYLFYLICFSFSIKKFEIINSYHLQYFFLLSVSLIDVEDILYTNKIQKYRVPTSIQCLEVLIENSQLFYIQGYLTLSLTNITIMNQYSIDQSLIQILSNSSVANKRESIKLKDLNFVGNILLKKSLGLVLSQLAIYSDKNQIIEIEKIKFQDNSFNQLIDDPSQTSSGLLFINSQQSSLYISNLLSQRNALTNSTNSYIYINTNLLQINNIVINNHNQLIQSFWNKYFNIDLSENYNQEIINQMINKVFTIQNKGGALAITTEHFTINNCFFKDILAQSSSILEIITQGLGKVTITNCSMISAQNIFSQIGENEGAISIYSKNSLLELKFQNITLKEIQNRLTPSILSIVPSFKSNTIIFQNIVIQNCFSLVNQFMKIEFQLLNLKSNIVSMKNISIQQDENQLIQYFQKMESITIYEIQKITYDNAIINLQGCNLRIKNFQFQGILLSSIIKVLDSYTIQLENLHIVEVQLFYPINIIHIGQTTSMQFNAVLYNIEIMNLVSFDFNKIKTNLIEYSKIVLEQKSCNINLQLLNQDLNAPIKIAWIFEQILLLSSQKGTLIYILSTNSQNRIKLIQILISNNNCLECQNGIIQFDIKQYLSIFIQELYCLKNNISNYGCVYAKAENQQESQMVIKNSIFNMNRGIIGSAIMAENVRITLVNLKLLNNIATSQGGALYLSINNNQFKIIQTIIYNNKAEQGGGIYLQGNGKLNKDNFIKSWMKLNSAYLLPNNLQEMPTHLSLSINNFEMKTIQKKINNKTSNSLLLQPYFVMEQGKVKLTKLLMLPSNQEISNYQIYNPSLLKSEKYLEEFSISYKNNLNEILPNFSNSTCEIFMQTLINDTLIDSTNIANIEYNFESKNFDLRFLSITVDPYKQNNQTNQIQIVCHLNDKSETLLYDIQIKGFMCQLGEFYFQSGCQICQPSQGYYSVTYNTTKCSIFDQNKFKSITSNQIQLKKGYWRPDYQSDLIEHCFKFVDFCEGGWVVSDDLCLVGHIGGLCEECDIYDIRGQGNYFKNQLNTSCQDCRDAINSVFPFVLTSIWALISTLLTLRSIDKSNKLFSSLKIRQRFVKIIFKLNQDHESILLKLFLNYVWIFSVIFTFNFQFSFSFTFINQTSNTSYFMANNLDCYLSKIYQNISLIYNRIITMIVLMVCQLLIIYMGFKLYSKIKHQKFNSSIISTTLLYLYVSNYAALIKQLFSLLAKRIISDIEYISGDVSLLYNSPSHKKWIIGFVLPGLGLIGCLIPSALFLLLYIQRDKLDKIKFRRHICYLFNEYNNENYFWEWIKLWKKTVIIIIMTYFETNVFVKASLLGLCLLLYQLYAAKQKPYILNNLNELDVSTGQICSIAIFLASIKYISEQQENKSTSVLIEMILVLLCLKLSYPFVIDLLRVYYKKYKILLLSNLYDALKIINCNLSLTLYVNRKLNQMKQREQKLKTNLIKLRIHLLQISKFQLESQKSLVNLLNSQSTSRLAMLGLDQNAQKIIRLETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKK0</accession>
    <name>A0A8S1PKK0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1200120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKK0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035911696" evidence="1">
        <location>
            <begin position="18"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="72" mass="8663" checksum="378D3D64A6C544A1" modified="2022-10-12" version="1">MSQKDIFELLLPTLVFASELLPSQMISQVLLVQQLELAQFYLLPTIYGYFRNSQKRKERRYFQIVLMMYNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQ64</accession>
    <name>A0A8S1PQ64_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Intraflagellar transport protein 46 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1260005</name>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T3560001</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IFT46 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000365">
        <property type="protein sequence ID" value="CAD8119675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQ64"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022088">
        <property type="entry name" value="Intraflagellar_transp_cmplxB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13376">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 46 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13376:SF0">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 46 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12317">
        <property type="entry name" value="IFT46_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8105014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8119675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="16027" checksum="D681B078494A9CFF" modified="2022-10-12" version="1">MQRQQIGAKILLSMLLKMLIKIQKKLQPVLMMWQKYIKKSHLHLVSYSKIMPDIEELMQVWPEEIKDQFQSNQLPGDNIDVGLQEYCQFSCNLLDIPAHSINNNRNIIESLHVLFTLFSEFKTNQHFRQNNDEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PU32</accession>
    <name>A0A8S1PU32_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1310061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PU32"/>
    <dbReference type="OMA" id="YMEQGSK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000323">
        <property type="term" value="C:lytic vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157:SF5">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="5"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8106582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="267" mass="31602" checksum="342364CA9F4F07D3" modified="2022-10-12" version="1">MLQELQSLKERKESLQKQRIALEEELNQLLSYLEQGSKKLSDAYNVKCQSTEFEIKKKEIQKQMEKESQEIVKNQQYCTDQQRVYKERADWIDKFNDKLKEDFKSCQKKEETIQIRSTVAKGIKRLFLLQLCTIFFDKENQRLLWGLHKIKYLNDVQSNMSNQEEDQLATSLGYLGLLLQQIAKIKGVTLKYEFKFKGSKSYIQQQNDTLYLFSQKPSNMKILKQSYGLLIQNLYIIMEELSVQKQWRIQSLPAIIGKIGEMLWVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXQ2</accession>
    <name>A0A8S1PXQ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1350042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXQ2"/>
    <dbReference type="OMA" id="HQMLNIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="49519" checksum="B094553C3B12F366" modified="2022-10-12" version="1">MIKRFKKLFQGQSEFQSFLTKSSLSKDNFVYKQEDVEILIPFLTDDQNIIEFFQVYQEETCQHYTIKMKMLITIHQMLNISDEFAQQFTKINFAYFNKPSQKPESIVFKQVTNEIWLLENLQIPFLQYLQKLALNVQEIKSYRNHHFRKPIHQSNLVVECFKLQNLVNQGLSLVPQLQTSLSNFPHDFLLKKLACNLYCELRQFQRQLINSLSALLDTNSRASNIEIFEFFREVQIIEKKTMTYYMLHKLFDPGRKLMPPLQLKIDLKSAKHLEQQALNEQLKINQQKQMRSQRNSRSNSLDQQQRVSQGFISQRERRFMMYSRTKKQDTIQEDIEMPTEEQQPSDKKKITQMMDNQANEIPKQFDNLENDNSDIQKKESSNESEINERNSQKETNDIVKNQESTSQADQDENQYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXV6</accession>
    <name>A0A8S1PXV6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1360017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXV6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="95"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8108117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="453" mass="53866" checksum="5361FC74B9936AE4" modified="2022-10-12" version="1">MNKAISCKKHPKFFIQWITILKEGVNFGCIKCLDQINNRRDIIYLPEIIENPSIVIPHLPINEQYISFYENLDKFSQEQLEKYYGDLETTLHFLCELLKNLIQNGRANLEEFKKKCKEHKEQLYQQFKIQEISELIQEFEQQQNQDAQDQIEKKLIDLKEKMNQDLMKEQSSIIKKIYNETSLAIDKFNLNCNQQIKDFSNQINRNIFSLKEFFKKSFVTQNTQKQIFSFFNSSQYARSNEIQLQNQSHTSLIEVDRSNCLFFTNNFPYYFSKIVPQYYLDHIIKQANYYMGKFLSYNNEIQLKAYSIFEPNSQEIVNKKHFWKLNPQLKSNAQLLIFKSNNDGNHIFGYFKISLVEFIFSYTHNEIYPKKQMIQNPFQNGGFFKNLQSKAQNEIDSFIVGDSDLQIHSNLIDGKSMLGQEFLWDSQKNDIKQTEYLFGGEKPNIQICEVFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q3X5</accession>
    <name>A0A8S1Q3X5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1410003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q3X5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="850"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="891" mass="106954" checksum="EFC279F444F4A5C0" modified="2022-10-12" version="1">MESYNLIYQIDKTKKMCFFITNLKICIKIQYENTQDKKTPELINLQIEKDCSQNQKTILSIIDFQSLQQMPFSLYNNYTNGVINLSIWNEGEEENLCYYQLRMYNFKGKIQLKSMYTFTHVVFQVEDSIYFHDCQLKKIQLILNTNQYEVFQNQEYYFLIDKNKKQIRMIYIFPGITHQIVTHSNIEIISAQQIGEQIIIYIKNQQSPILISKKKFLEKIQNNYLSQKLKQPGEILYYQEISKYKFIQYQNIFAFEQNEEVRCFQFSDVQIVYIKIYVKAHFMIFAVQIQTNSILLYHFDQFEIHQFDNYTLEDYSFSSPLRYSNNNFNLAILTVKNQFSFITLLSFNPFKISLMDVKDTDDTYFEFINNALFYFRNGQIRQHYLNKIVALIETNMSYQQTLISSHELKLMSTQRLDDDIPVKILIQNNCYQIQPLQNTISLNIKKSQNLQIKISEYFQGPINNLFIKKNQKTKIRDPIQFIQEINICNNENFFCIKQQQLQKKIETQELKSRFKAIILEDNTVFQILYPNTQDQLNYVLWIQDHQFLWFSQMEDELHIQLLYCVFKDNENCLEIQSQIININIIQPLQQNIIKTKNLVRLQNTLNQIYLFINDQNFSAIDLPGDYVDIQYILQTQDNYVTFSYSENGISFQFEISIYLIDSNGFFLMFSKIITKEIEQIIPLFNRCQQIKLVSSRYNSNVTTVNLLLFINQNSFMIKIQIDVHNNSILSFHLTKQIRNPKVEYQSTFYYVDENYLIMNYLKKTMFFYDLQEDRLFYDYIYRLKSDIQMIRINTTHFISQNGLHVHLGIISQYVIDFQNLDDSNFNFVLFAENDVSNAEIPIYIIQEQPFYYHNPNIILIQLTNFFVIIVYLKRQYCKNLQRKNQQKHMGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7U3</accession>
    <name>A0A8S1Q7U3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1470001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="EFILYCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="41"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="56"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="90"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="124"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="158"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="192"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="226"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="294"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="328"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="362"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="430"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="464"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="498"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="532"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="566"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="600"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="634"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="704"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="738"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="806"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="840"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="874"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="942"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="976"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1010"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1044"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1078"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1112"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1146"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1180"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1214"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1248"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1282"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1316"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1394"/>
            <end position="1427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8111235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1481" mass="173538" checksum="87168756FE05CFF1" modified="2022-10-12" version="1">MNRFEEALEYYDLAIQKNPGKAEYFNGKAIALMSMNRFEEALENYDSAIQKNPENADYYYGKADFLQIINRLEEALLYYDSAIQKNPEKVDYYNGKAIALLKMNRFEETLQYFDSAIHKNPEKADYYNGKAIALFKMDRFEEAQEYFDSAIHKNPEKADYYNGKAIALRSMNRFQEALENYDSAIQKNPEKADYYNGKAIALKSINRFEEALEYYDLAIEKNPENANYYYDKADILCNMNRFEEALKNYDSAIQKNPEEADYYNGKAIALRSLNRFLEALVCYDSAIEKYPEEAEYYQGKAIALMSMNRFEEALVQYDQAIEKNPEQAEYFYGKAIALFKINRFEEAQQYFDSAIHKNPEQADYYNVKAIALQTMNRFESALKYFDQAIQKNPENADYYYCKAGNLENMKRFEQALINYDSAIMKNPEEADYYNGKAIALRSINRFQEALENHDSAIQKNPENADYYNGKAIALYKMDRFEEALQQFDQAIQKNPEKSDYYNGKAIALKNMNRFESALEYFDQAIQKNPENADYYYGKADILKNMNRFEEALENYDSAIQKNPQEADYYSSKAIALRSINRFQEALQNYDSAIQKNPENADYYYSKAIALFKMNKFEEALENYDQAIQKNPEEADYYNSKAIALRSINRFQEALQNYDSAIQKNPENADYYXXXXXXXXXXXXXXXXXXXXXYDSAIQKNPEKADYYNGKAIALKSINRFEEALEYYDLAIEKNPENANYYYDKADILCNMNRFEEALKNYDSAIQKNPEEADYYNGKAIALRSLNRFLEALVCYDSAIEKYPEEAEYYQGKAIALMSMNRFEEALVQYDQAIEKNPEQAEYFYGKAIALFKINRFEEAQQYFDSAIHKNPEQADYYNVKAIALQTMNRFESALKYFDQAIQKNPENADYYYCKAGNLENMKRFEQALINYDSAIMKNPEEADYYNGKAIALRSINRFQEALENHDSAIQKNPENADYYNGKAIALYKMDRFEEALQQFDQAIQKNPEKSDYYNGKAIALKNMNRFESALEYFDQAIQKNPENADYYYGKADILKNMNRFEEALENYDSAIQKNPQEADYYSSKAIALRSINRFQEALQNYDSAIQKNPENADYYYSKAIALFKMNKFEEALENYDQAIQKNPEEADYYNSKAIALRSINRFQEALQNYDSAIQKNPENADYYNGKAIALRSINRFEEALLYYDSAIQKNPENADYYYGKADILYIMNRFEEALQYYDLAIQKNPEKADYYNGKAIALFKMNRFEETLQYFDSAIQKNPENTDYYYCKADILKNMNRMEEALQYYDSAIQKNPEKADYYNGKAIALFKMNRFEETLQYFDSAIHKNPEKADYYNGKGINQRQVFCLATALFKMNRFQEALQFYDSALQKNLEQAEYFNGKAITLFKMNRYEEALEQFDQAILKNPKNADYYIGKAIALSHLSNFDSALKYCKCAILKDPNNANYQLVENQLKLNNHKFNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QA93</accession>
    <name>A0A8S1QA93_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1520082</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM161 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QA93"/>
    <dbReference type="OMA" id="MYENPLI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051655">
        <property type="entry name" value="FAM161"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019579">
        <property type="entry name" value="FAM161A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21501">
        <property type="entry name" value="PROTEIN FAM-161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21501:SF1">
        <property type="entry name" value="PROTEIN FAM-161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10595">
        <property type="entry name" value="FAM161A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="629"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="780"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="854"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="446"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="332"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="811"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="868"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8112669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="895" mass="108361" checksum="B38CD9D088D74A32" modified="2022-10-12" version="1">MTDQLFVGQLQQKVGELKTEYDLQRSIKDNTINAKIQRSRDKICEILRRVQSKRPKLQTEESEESLDGNIPELKKPYESKYNEEVLQRRHYIFQFKMYLRRKRERLQREQEFKLLKLLDQQTEALQKWVTKDPNDLDMFDFFYTQIDPHRKGQVHHLQLKNFMYENPLIMTLFQFDRQSLQLALDSFPVKERFHLKQDEFIQFLQKYQKVKPEKGFREYVHLFDPIHKQVNLFENNPRILLLEDISKIQEAFDLVSENGLATVDEAIKSIRQNCELFVYAVHVVVFGLNLLLESVLQQIEIGYLNNPDEQISWNQFMYFFDNLPNDQDEEQLPQQQQEESISSESSDDYFYKMTAKYQIPPPPETEKKKVKKQSVPRKPFQEINVDRMQFTIPKKTGQEKRDVNKQPSIREKWVMEQVRQKQEEIEEAYKFRPFKAKAIPYKVKDKNYYNELLEREEIRRRELKERCKEKTQSMQKPFNFEDRKREQPIKLPEDQEEPYKFRANPIPWYCSLKLYERNIQEQKIKSENRKKIRKFWLQENSKLPPRMQEWIEKMKLKEQEKQQELLIKSYHESSRKVRAKSIPNFNKLHDQFQKQLDKKKKKIKTTIPIGPTFHESKKKAQRDYLDEKSIQKEDPLKKAKKAISTKPKIQPRSTDKFNAYVDYIFTMKQNQLALDQQNQEEQNQREDKKQLFRPRVHQSWAIQDHNQVQYERQQQKFIEQYQKRRQEEEDFNNMMKEIFVRVYQRPLLMEVEAAKSQQQPQEEQEQQEQFDYMNPIGEQDVEESERISQQDPEGVDQVGEQAEQSLREYDGQEIDGEYDDQIDIDNLDPKLLEKLQKDAIAKQYGLTEEQMEQIEQMGGFEAVQNMGYEEDDDDDDQEQDDQEELQNPHYKRHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QAZ2</accession>
    <name>A0A8S1QAZ2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1530015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QAZ2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="72" mass="8297" checksum="9048602128488E47" modified="2022-10-12" version="1">MIFDINSQNGSSSQIIYIGSPYISYYFVQMMHKQRDILIFIRSKNQDLSILDISSGILNDSQFVITMKLVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBS0</accession>
    <name>A0A8S1QBS0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dephospho-CoA kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1510053</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000156">
        <property type="protein sequence ID" value="CAD8112394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBS0"/>
    <dbReference type="OMA" id="RWEMYRA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02022">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00152">
        <property type="entry name" value="dephospho-CoA kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8112394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="36184" checksum="046FCAF406455268" modified="2022-10-12" version="1">MEQLNSEEQVIIEEIIHSEHTEIHIIEYICYPFYLALLCLLCILINLNKRKFRRRYRVDEIFLFIAVYLFNVLITWNFFDFFDKIVRFIITLIIIFGIQHYIGRVQIVGITGGIGCGKSTIAKYFNEFLKVQIIDCDQIARDIVEPGKPAYKLIVQRFGLSILAGQQDGQPIERQKLADVVFQDSQKRKQLQAITNKFIFKEIAKSIWKICFIQKDQYVVIDAPLLFESKVLEYFCFPIITVVVTSQEEIIKRVKERSGLSEEQILHRIESQMKAEIKIKKSDIVITNDKSEKSLIRQVQEKVFEYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QD23</accession>
    <name>A0A8S1QD23_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1510016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000156">
        <property type="protein sequence ID" value="CAD8112320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QD23"/>
    <dbReference type="OMA" id="WNKQMIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8112320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="58530" checksum="9437D3C2FA62C23D" modified="2022-10-12" version="1">MNKITLKFIDKAIEQKYQLEHQQPKRKTHLKILLIFFIMLQIIKLIIAIIINNYKAVYPTLGMMGFTTLSKFFNIDKEYHQRALIIYINICFSIYVIFFDPQSDSPTMYFRGANQMVINIINIIGIEFLDSTITIILLYSLRILHLILNTSSIDITSIIFGFGANISLIIMVYFYHKAIRSQFLLSKIDQRWENILKQIIHNQQFILIKYQVEKLQFQSVSSTFSKTIQSQEEVIKFLREAKVDNGSLEQYLFHKLQDFSQQYIEIINHSINVKFDKQLLQVYFSIFFGNQPTILIQLKQSQLQLKNKDVQKVCQLYLKLLTVFIKIIKENIPFDYNQFHNLANKIQFQNKITSIWNKQMISKTISLKKSILKIQKFCNPNTKIQLISSDLIIYTIPKIFHLLLACIVDSSISELIIIKCETVETQLKLIKIQGKFNIRKLNSYTIKIKNCLLLICKEINVQQFCINIELNDEILQPFTNIIMPQLHFNYSNKKLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QHV2</accession>
    <name>A0A8S1QHV2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="11">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T1630040</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11"/>
    <dbReference type="EMBL" id="CAJJDM010000170">
        <property type="protein sequence ID" value="CAD8115222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QHV2"/>
    <dbReference type="OMA" id="QCEYICA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2230.10:FF:000018">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000535">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000536">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DUSP" evidence="15">
        <location>
            <begin position="29"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="14">
        <location>
            <begin position="301"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="13">
        <location>
            <begin position="364"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="471"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="475"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="499"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="511"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8115222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="998" mass="117096" checksum="B8131C70E9F59E94" modified="2022-10-12" version="1">MGRKHKKKGGKNKRHQPPLPPLGEDFKQNLYLEALEGINKLKLSKDLFLQKQVKEAYLLSKIWLETWKTHVGYDAVLKGDQPGGKKYGRQQIGEINKDIIANEELFHSAPELYFYFDPPLNDVQPVKDFILITQDVWEFFYEKYKGKAVRRIANEQGEFNLMTISPYAIFMTQEKLNQIGSLNQNQLANSVDLLRMRKIQLYSDWKFKELEIYCQQIMESQDIQIWLMNPIYEQNQIKLQLIKTINQNKNILLGEQLLEAEYQQLQLSQFLKNHFILVVFQNEVELSQLDVKFELNQNGCCFNCGSKALLNYSCACKKVFYCSDTCRYKDQEFHSRQCTKAYDSQDDDDFDAITYETIQNGQGVGLVNLGNTCYMNSSLQSLFSSTHFQEFLSNKQYWTQNTIKRSVQLNYKLEKLLKNLLTQNKAFSPYSFRQQLARKYPFFGNNYQQDAAEFILYLFDALNEELIQKDDKQKPQIQLQQQLDLGDGDKPEPEQANSDSEDDSSEEQENTENQSTQQQQIQFHKKVQQNQINNDNYEQALELIHKLNSSIIVKNYLGITRSEIECPICYQKTQSYEQFLILPLSLNAVSQSKHLVDVFVISNDWYKPYEKIVYEYQPNQTMLLDVKRDIGEKLGIEAIYLLGAFCSNNQIDMYLFHDEDQLSKVLNDNQNCYFCLFQLDQSIYSITDEDQYFDVMIYSYEKKNAFSYQCEYICAAIPKILIIKKQFTAVQIYQQIWNKLGEHSKSDQLMNIQIDEPQQDSLLNSQQLLEVLNYQIIIQTRLPSKTPCPFTCGKQFKKDQKPHCCELPFNNSNTLQQYLEKANYANAHTSLSIEFVENAIPKSLRIEFEPKTNYHKHDKSLNLLNLIDNFQSKEPLLDENAYHCTICNDLTFAIKTMSLQKLPDQLIIQLKRFQYDEHKGFQKNNILVTYPEAITINQIDYELYGVVQHFGGLDNGHYTAYGRRQKKWIEFNDDSTKEVKENDVINDKNAYILFYQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJR5</accession>
    <name>A0A8S1QJR5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1650081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJR5"/>
    <dbReference type="OMA" id="FMPNDED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8115636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="357" mass="42425" checksum="91FCECC96DAEDF1A" modified="2022-10-12" version="1">MSLTFINLEDSPDKPKPFGLASGMIEDFLHNPLEMLRTLKCDKISGSWDLEKIREILNKINDLAFQNNVSHDIIKPRKISLENQKLIDQVDDFDKLYSSFRHSQKYDNKSERIQKVIKDFKNRVIYRSVRPSSVQPKTIEQPKKCKLMSKEFEQKLNNINEKYGMKGSQMTVKSKYMNHYSLNTNNQQTNTECIRPQTTTCSARKTYSFRREKTFDLDQKFQHNIRTSRLRAQSGHQVQNFELEKQNQSIIARIMKKSEVRLHQKVNLKKYDLENHDIQPTFMLDDSDSQTKRLMRCIDFNPLNSIQQEYRNFMPNDEDFIVKLSFQQIQSKEKRQNKQKQKVFYAKAQSNYIQDPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QRG9</accession>
    <name>A0A8S1QRG9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2350003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000244">
        <property type="protein sequence ID" value="CAD8118349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QRG9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8118349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="122" mass="14429" checksum="AC4BFC084DEF187A" modified="2022-10-12" version="1">MNNLQVCYELLNDSYRNFFLGFGGNPTIIFHYSFIKENSQGNFREVQNVGVQVFFENSLYAIIALSYFITYWRWLTVSIVILSVFLNIGNLLIFIIFIHKENICKDSKLICKIKWDLINQNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JLU2</accession>
    <name>A0A8S1JLU2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JLU2"/>
    <dbReference type="OMA" id="CFIKAGL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF1">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="96"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="289" mass="33158" checksum="C8C8CA3EE39859AE" modified="2022-10-12" version="1">MNQSKIWDAYSKSYHNIEKNPAGFQFSLLNMLRVNEADEILDAGCAGGHLHQYILQQRKKDCRLTAFDFSLEMTKIAAARMIKYLNNPFNSQIEDISDQEIENVDLNSPLLEQNKYKVFQGDVQSLPQLNDNQFDIYISNLVLQLVANKDQALQEAFRVLKPGGKIGIVIPVVLGNVPMLSLQKCFIQAGLIPNAAYKPTELENREGLINFIYKHGFKEIVCWNQTIPFDLFDIDDNYIFPQFREILDKASKEQVDQFYSLYKSEVQNRLNLNLPFTVEALSLIAKKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNC2</accession>
    <name>A0A8S1JNC2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNC2"/>
    <dbReference type="OMA" id="YIITWDF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="40011" checksum="7867354949BB6D9F" modified="2022-10-12" version="1">MPESHSKSFSYKLLPQSSIHQNCSSRAMAINKNKTLLLVQTESFIKCFAFKRGEIKLLQLFHFGNATVNTLNFQMQKQNIILSSDKFICKYSSNLMANPKYTQKCFGQFNTMCLILHPMKEDIIIIGSKIGPIHFLFTNQIGSNKPFTQTIQEHNNVLGLSINDQGTRIISCGKDNFILIIEPNSSNFELQQCNWIVKQKLIVEQFGYRICFINNNQFTFQPSNKSYINYYCEINGMFYKQSQHQIPSNKQDSFCIFQQVYNQQKSLIFNIYGRVLQIIKFSKEKSLNSDQTDQLFQLEQFIDFGQRYNPWIYGIISEDAEYIITWDFDSEQIQIRQFQNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRH7</accession>
    <name>A0A8S1JRH7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(m1A58)-methyltransferase subunit TRM6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0080191</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRM6/GCD10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRH7"/>
    <dbReference type="OMA" id="TRCRPYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017423">
        <property type="entry name" value="TRM6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945:SF0">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT TRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04189">
        <property type="entry name" value="Gcd10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8044625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="358" mass="41804" checksum="DA46A3C2386BF7B0" modified="2022-10-12" version="1">MEIIEEDTWVCLEDNRNESKIVKVSEKKMVGWRKHKFDIGVILHKQYNTFWKIDKSNTVVEITSDQFYDNADLDNQIDDANIEITKTNKDLFDFNDSQKLTQEEIIQLKQQKEGEEIVDEVIQNSKTFEQKTEFSKAKYLKRKKEKYVCCFMVLKTTIDNVHKTQFLESPQKLNFMRIDSLAFFMQLSSITQNSNILMFDNANGVVVSAVAERLDGEGSISTVFWNHSKSNLRDYLPIQQMNFNEEILKSIKLIKNLEDIEKNQNKYTHLLIAGEFKFTEIIEKYLNLIAVGGIIVIFSTYLEKLHEVAEQLLSSGLCSKIDVLDSFLRYHQVLENRTHPKVEMNAFGGYLLQTYKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JT67</accession>
    <name>A0A8S1JT67_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JT67"/>
    <dbReference type="OMA" id="QKFTEYN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="55933" checksum="D661047CB29B166F" modified="2022-10-12" version="1">MKQKPSYWKSTHFAASVQYLNFLIECIIFPLALILYINHYKMDRNLYDNWEFKPIIEIQKVYGSKCPSDLETFFAYNYPETSSGCDCGFGEEDENVEIDYDKLKNEHCQESEIDEGCDEIASIQSQDFVIWKGYSICGKRSQYTYRELFYERNCTAICNGVCQTKNNQNDCGNYEDLISKENITQSYYNFTLSYGIDVCYENGESINQYPLLESAYKCVIQSSEYAYIDEFDQYLLFEENEYPYDSLPGLEYFDEIPKFGLFGQKFTEYNCTIKQIDVSNYRDNYIQLVFLIALIMAGCLMGFGAIFHSAIVQNLTRKGIFKHKIYKVTWKFYCPTTFLILLHMAHLAIVGTKLGFLAHFRTTMHNYIENKCTDWSNILNLVYMRDIIDAELMPLCIPELVLTFVAMLLEILQLCLFDRRIPETIASPTLKGRESPSKSNNFILNFGFDKDENIEKHVLDTENIRINKKNEQLKLYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JUM4</accession>
    <name>A0A8S1JUM4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JUM4"/>
    <dbReference type="OMA" id="MPKINCY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="50020" checksum="B7B9B627D4FEBEB8" modified="2022-10-12" version="1">MYEQPEASPGFNRNQIEQQSDSQREFTYQQYAIPPQQSLTDEQKSGGNLNPFVFTSYNMPKINCYNHLGQTITNFCQSKECRLPLCPECVKEHVNEHSQFKTYPKLECLENILTQVHSDICQQANQINNALQNIQKSKAQAYSQKAYTVEKLKEAKKRILNIIEQYFDTLENELENKQKKNYENFKRDADALIGALKARQGAYINFLDKLQQPECMYSLLPYLASPSYEDNQQYIQVANKFTTRFKEVQSEITFDQYKSVQLSSHLSEIVGVIHQDLTEYLDMHKLAPPEIVKSKALTLKSQNNQLQKKTQSPNQKQINQSKTHLIQENNDQEIIKHNPNQNQLEQTIRYQQINPQSIQIQQHQSPSNFNSLNQIYPQQLTQQQPQYYSNQPQYSVQYPYNQQRYQQQVPYQQYQNTINSMQKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JUQ1</accession>
    <name>A0A8S1JUQ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0100021</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JUQ1"/>
    <dbReference type="OMA" id="EICSPKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002024">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="295"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="295"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8045775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="521" mass="60741" checksum="B920574EE306D9F9" modified="2022-10-12" version="1">MNRQEIINIKPEKKISNYAFNLKAILGKGSFATVYFGREMNTQLPVALKVIDHSKSINYAQLYQSLHQEIEIMKKLKHPNIVQLYEVYSTSNNTYLVQEYCNGPDLKQYLSQKKVLEEEQAIKMIKQIANGLKEIVNNNFIHRDLKPANILIHDGQCKIADFGFSRPLPLECIMESLVGTPLYMAPQILTKQQYTSKCDVWSLGLIFYEMLFGTLPWMATNYMELIYRINNCKLTFPKNNKISKESLSFIQGCLHKDEVQRLSWNEVFLHPLIKPQMKIPIDPGINEIFSPQFSTHRQIQSNDKSTLPTLRERSCSGKNPLNKQSNEINIEQKQELPPATSSQQSTEGEENTKLGKYYLPKQQEKFRRTRSQFENIKPNQTEINPKRYIQQPLQSNCNIIKQTFPSKKRLHFELFQKNNAQKLKLNSETIIQLLDLITSLEKDVKNQDMDLEAFLNKERIAITNGQQNQALRIKIAQKYVEKLESDKKRKLETYISLLQKNQNYEENNIQKNNVAHLLYQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JV69</accession>
    <name>A0A8S1JV69_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SKP1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0060454</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SKP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JV69"/>
    <dbReference type="OMA" id="DKYTASM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18322">
        <property type="entry name" value="BTB_POZ_SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000026">
        <property type="entry name" value="E3 ubiquitin ligase complex SCF subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028729">
        <property type="entry name" value="E3_ubiquit_lig_SCF_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="5">
        <location>
            <begin position="3"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component dimerisation" evidence="4">
        <location>
            <begin position="111"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8044370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18503" checksum="32F225144B62BCDC" modified="2022-10-12" version="1">MENKVKLSTQDGVIIEVDKDVACKSHLINTIIDDTGSEEEIPLPNVKSSILKKVIQYCELHRNDTPPEIEKPLRSNNLSDCVEQKDADFIDIPNLEELFDIILAANYLDIKSLLDLSCAKVATYIKGKTPEEIRKTFNIQNDLTQEEEQQIREENKWAEETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0G9</accession>
    <name>A0A8S1K0G9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0100405</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0G9"/>
    <dbReference type="OMA" id="FEPKVWA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052056">
        <property type="entry name" value="Mono-ARTD/PARP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14453">
        <property type="entry name" value="PARP/ZINC FINGER CCCH TYPE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14453:SF67">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Macro" evidence="7">
        <location>
            <begin position="2"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8046510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="409" mass="47656" checksum="6E79E71733CC63AB" modified="2022-10-12" version="1">MQLSTSIAKISNINVNIQSGNITEQQDVDAIVLPTNPLLFKEPGVSAEIFQKCGQDKLEKALKKLKEKEKQKRKQENGQIEQPGLFNNKKEKQLILDFSQFLAMKQQEEQEEEIEDIRFNIGYVDCVKSYKLKIERQIQNILIVVEPENFNEQLIVQSIKNIIIKSHALGFKKVAVPILCQFDDNFEPKVWAFQYKKAFFDVQQQLQLKNEIEIIIPCHTEEILYGFQKTFQSSEYVKTTNIYITDISNVEVLVNPVNQNNPYFGASGKIIEKAGSALEQDIRQQVVRGNKWILSKSFRLQEQNIKYILTFIGPVQQRKTRADKLQYFFQKILQICNDQLKVESICIPLLAIPQAIFEQKENQNFLLNRIPLALKDAILEFEKQKKKHKQILISIQDDQLREFCIQLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0H2</accession>
    <name>A0A8S1K0H2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0060010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8043928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="358" mass="42457" checksum="BBD3613E0BBB19EF" modified="2022-10-12" version="1">MFANRQQMNIKCLQLVFQKAQMEEKQSIRADKNLYVMADSEKEIWQILQKIQFNEYLLRICFITNQIFTFQIIQITFRNQKNLSIFKILPKIYLYKERVNMIQGIANQFIDQKWLFHKYSKIYSIKSKDKMGNSTISQNNQLILVMIVYMEQLVMMDNSQLLGIINLIRLKLKNIIRRLINKFGQVLLKIIFNINYXITLELKVLLLLNLTNLLASRAILSKISLMNEFIMFMAFLETPISGYTYLTTLQKYKPNWYNSEKSDFSNVLAINYDNSALVVGIREKIQSFKILHYKEYNIENLTEQEEEELFASDGKALRKLLICDGNICQVTTLQFQKSQTCELLPLWFRKFDKYLGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K143</accession>
    <name>A0A8S1K143_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">40S ribosomal protein S16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0130334</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K143"/>
    <dbReference type="OMA" id="WPIEMAR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000007">
        <property type="entry name" value="40S ribosomal protein S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001749">
        <property type="entry name" value="PRK00474.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8049220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="16434" checksum="36840DB3B5C40261" modified="2022-10-12" version="1">MSTPQTKSKLVQTFGRKKNAVASASVREGKGLVRVNGAPIELVNPAPLRQKALEPLLLLGQVRTGRLDIRVTVRGGGSTAQIYAIRQAISKGIVAYYQKYIDETQKREIKDLLLQYDRTLLVADPRRCEPRKFGGKGARARRQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1E7</accession>
    <name>A0A8S1K1E7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0120466</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1E7"/>
    <dbReference type="OMA" id="EMHETSP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF408">
        <property type="entry name" value="OS09G0560450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="13"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="68"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="122"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="188"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="247"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8048440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="401" mass="44699" checksum="10E7014B9D2A2321" modified="2022-10-12" version="1">MFNSVRRYLFSTGSLFTWGQSNGSLGYAVGSQQQVKGVSMPQLVNGFNNNVAQVQMGQNHSALITKDGELYTWGTGNSGQLGHNNDKDYNTPQLVEFFTKHNLKVNQVALGDYHTVALTHDGDVWTWGYGGKEQNFLMDLLFLQVGALGHGDSKNRYTPTPVKALRSLKKIKYIQSGLRFTNAINENNELYVWGKGDYGVFGDGNNKSFNIPQRNEFFEGYLKKELNLSIVKLKSCNNYSVALMSDGNLYGWGSNDFGQMGIKNEIGVEIYETANFPTQVVRDKFGNNKIVDFVVAEDLVAVLLDNNEVYWSGSKAEYSPVRFPLPSGIAKITNIGCCYRCIAVATEDGKLYYRKKFFGEGSEDLSTGIITNDVATVFQNQNAKVLEFGGAYRNRYAIVQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2H4</accession>
    <name>A0A8S1K2H4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0130108</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2H4"/>
    <dbReference type="OMA" id="MAGIWRP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="281"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8048856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="347" mass="39867" checksum="0C02EBFFE26FE145" modified="2022-10-12" version="1">MGNCSFSNSCEVVESNISIKDFDLLKILGSGSSGKVWKALNRTNKQLVAIKVMSKLLILKQGIVSLVINEKNILSQIKNPFIVNLNAAFQDNYNLYLCLDLMSGGDLRYHLNKKKQFSESQTKFFIACIFISLDYLHQQGIIHRDLKPENLVFDKQGYIRLTDFGMAGIWRPNSLDYVCGSIGYIAPEMILQKQHGIGVDYFALGIIAYECMLGKRPYEANDLKQMKDLLLSQQIQLKRSELPIEWSIEAGDFVNRLIKLNAEDRLGTNDPQEVMNHPWFKNFDWNKLISKQMNPPYVPKKSIGYSNYQKCKQSKQKLINIDILNSNQQKFAGYHFNPKKNNYIQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3G1</accession>
    <name>A0A8S1K3G1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spt4/RpoE2 zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0140190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3G1"/>
    <dbReference type="OMA" id="FKANGCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.210:FF:000014">
        <property type="entry name" value="Transcription initiation protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009287">
        <property type="entry name" value="Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882">
        <property type="entry name" value="SUPPRESSOR OF TY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="2">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8049839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="93" mass="10542" checksum="3FCFE92AF6647EC0" modified="2022-10-12" version="1">MQSESEMSEYDEKKFILPKSTKDLKACQQCGMVLTVEQWNREVECPNSCNASQTKLFSGMICVLKPSQSWVMRKLGNPKNIHPGLYAIDVQAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3S1</accession>
    <name>A0A8S1K3S1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0140251</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3S1"/>
    <dbReference type="OMA" id="EHESIYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000079">
        <property type="entry name" value="Coenzyme PQQ synthesis protein F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="2">
        <location>
            <begin position="61"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="3">
        <location>
            <begin position="395"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="4">
        <location>
            <begin position="857"/>
            <end position="956"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8049959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1058" mass="125214" checksum="6F3EBEEDD3DF35D0" modified="2022-10-12" version="1">MHPVVFYLLSVAALSISTGIGLFVVNQQYSRQNIYPQVSKAQCDHRNYKIFTLPNQIHVMIITSNRTELSAVALKEETGYMNDPKDFQGMSNYIQQLLQTSQKYPQADLLRKIIMLNAGKQMRIINEHESIYYAQVQNDAFFPLLDVISRLFIDPLFTKQQMDRVLQNLHAQWKSMELFDYSQQLISKSRHPYSQFYTGNLKTLFEIPENEGKNPYIEAKKFYRSKYSANRLSIVIYTNEELKKRVTELLFEYFNGCVDKQLQKITYKQFGLPLKNQRFFYIQSKVKELRIEFDFDIENNHLTKSLEFVILMLMKQFEKNFCDEMFEKKLAFKCYYTLKEIPKEFAIFKIFLHLPKQTKIDEILTSLYKRINYLKQQDNLEQYWDHLLLTQEINFQYSNYDIENIIQLSSNLKQYFSKDILQNKLLKKYDKAAISHVLQQMEPQNMTIILLDNFYDSYQVDMGFMKFGNSFAQQSHCIEEKKKIFKGKVKKRYRVYEDEINDLIIDRDHPIDLSEEEEFIVEPDDVIQTEQDYDIDKGYSIPEIENFSYHQLFGLHSFEAGITANTVQKLLNIKDTSLKIVDDSHIPTTFDLLPQSPDNQIIKKKKKNFKYPELLITTQAGNLWHIQDTINGVPKVQFRVLARFLDFESLGENFIAKEIVKLNFKIPKGFNVASIQIDIGVGQIEIIVQCFTQQIEEIAKQVMKNYQEAFTINKEDFQWYIENLEYQDRYWEKNTTTIEKALFYAKQILTNSQEFQQHKYDVKFEKIQETLKKLWDNQRFDILLYGNIDADSSKSLFESIIPKGQSKSKSFFTEETYKLSKRAITYRLAKLKSESIVTLNLYQGSDPSLVQYVYMRLLQRLMKFHIEDYLFNQLQLGFAVDVSLIQVNHIDSIVIIVQSNSYDPEEIDFYIEESLGYFESFLNRLPDKDFDGIKYAIQKQLQQVPNTLEQQAEIVWSSIRDGSYYFTQRECTILALEKISKSDLMKYFLIFFKARNSKLSLQLFKEGAQIPMEVLREQDTFAQKTSDLILNQEQFQQAIKETYKFKSKYIEQAPSHMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KA16</accession>
    <name>A0A8S1KA16_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KA16"/>
    <dbReference type="OMA" id="YVTSEYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="281" mass="33359" checksum="8350173FF72DD908" modified="2022-10-12" version="1">MSLKSQRSKSKQTQNQTLDTSYQNKSHSDKRTSRFLASLELNNENSNSNSKQDNPEKAFFILLDACSELVQLHNDTHKQEIDQAIKQNQTFEQVAIIVHSKLQLLKRDYVTSEYQLIQLKESIISKQQQQNQQNTFDLYQKLHDQNQRWKQLYFQEARQLIEVTKQQKQFTIELQQQLEYINILELKQETLEQKYLELFELIHSSNQNQNIDIKKYCNTLQQALNDKTELLSVQLQNSRKQCIDLSKRLQDEIMKVQAAQELRIVDMQYIMSLENSIDQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KBZ6</accession>
    <name>A0A8S1KBZ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KBZ6"/>
    <dbReference type="OMA" id="IQARHTN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029147">
        <property type="entry name" value="CFAP77"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14825">
        <property type="entry name" value="CFAP77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="216" mass="24896" checksum="0E8C141DD29A143E" modified="2022-10-12" version="1">MYTTPPFSHNYSNPVLMKDDVGKPKPSTYNLPKQDYIYGLPLIRDKEGAKEVTMTWKFHQESQDRIPNRDFAELNKMSVFNGSLTAHDMYKFRQTHDARLQVKKGTNIQAIELPEEEFRYGRKNRPSTPMKLVMGNSYGIEAASITLDKYYKRAGSQESKMTNTIVRPNKASQLFHDSNHKKLAVIKGAEKKEPFKMEKFKTVAARTDTNLLAKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KG61</accession>
    <name>A0A8S1KG61_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0200221</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KG61"/>
    <dbReference type="OMA" id="ACANKTQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000023">
        <property type="entry name" value="Probable uracil phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="10">
        <location>
            <begin position="56"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8053223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="266" mass="30002" checksum="84C544DF8BE5D3EE" modified="2022-10-12" version="1">MNSRLLKIFETGFLGLIGFTLIAQQQKRFQCAAPYFSFISHSELQQFRLRFPSLYVLDKFQVEHLLSVIRNQTTQTKDFRLYSDRLIRLLMEKAISEHSKKLTPPEGSQNQTPAQEIQFENKQFCVVVMVRSGNAFLSEALKVLPGASVGFILVQEHPQTKDPQLIYCKFPEDIDQKQVILTDAMITTGGRISTAIKALQSNGVNQENIAAVNIVSCEKGLSKVLHQFPKVKVITAGVDYALNTIQDHRFPGVGDFGDRYFGTVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIX2</accession>
    <name>A0A8S1KIX2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="158" mass="19136" checksum="D7BD4CA59ABC1919" modified="2022-10-12" version="1">MNYTKKYKKQEDFLLNMNGEVLVQKDLLDGFIMIITNQNHIFQCLEKNLIDIQILLYINIYIYXLWGETNNENTHFQRLFIRSSILGDTLWNPFSKQSEKFYEFADRLGQMEDRMNKYGFPVSPFTHDYCKRHTKICFPILYTEQEQSVEQQHKFKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKG6</accession>
    <name>A0A8S1KKG6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0220140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKG6"/>
    <dbReference type="OMA" id="EYQWEDN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8054653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="45669" checksum="5AE21CB44333DC04" modified="2022-10-12" version="1">MGNCCNGGGQVMERNVQIEIGQNISDKQANSDILNKKAIIIQAHCRGHIVRKKVKKIQEQDKIGNKTSQNGSSKGKNNNNNKNSQNKRIAQEPKEEIYQNQVDLNRIHAPSEKYKKLDRMPDYLTAKTKQVLSQIESFVYDQDKDEFKELPFLEPYELDGGSVYKGQWKNGLRHGRGTQIWQDGSIYEGYWYQNVACGKGRLIHTDGDKYEGEWRNDKAHGFGKYIHMDGAQYVGYWEDDKQNGNGKEIWPDGSSYEGQYKNGKKHGKGTFKWADGSIYIGEFDQNNIHGKGEYQWEDNRKYVGEWKNNKMDGKGVFTWLDGRKYEGEYKDDKKHGYGEFQWPDGRMYKGLWANGKQNGIGIYIGSSKVEKEGEWQDGKRIRWIRKGGLPTKEEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKQ5</accession>
    <name>A0A8S1KKQ5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0180152</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKQ5"/>
    <dbReference type="OMA" id="HETIDCP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="473"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="699"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8051534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="817" mass="96768" checksum="C07C1ED902162AF3" modified="2022-10-12" version="1">MKSSQSDSIEEESGNWNLSSPQGPQISLRNTFANNQPDSSVGSLESERLQGKSEQQKQQFTIELSPEAQPIIKRTIIRTNPLQKLHQIAAKNRLVKQFKQNLLMNSYILSKDYKDKIIQFEQISQNKNLDYLIRKTQISILPVFEAYSNLMKSWDVIMIFQQLLQLWFLPFIISFYGFDSNIDIIREFLILMILLDIIVSMNREIFYKGNYIGNRPQIFRQYTKNGLIGDLIQLITWFCFLFIYKNIEYKAYLILLGILMVVCCIKSLLRKTEYYIDSYYNKGNLSNFLDLFILILQIYFVAHYMACIWHFVGEMGISFEKSTWLSEYGFLDESITTKYNYSFYWATMTMATVGYGDITGRNNYEILVSNIMMIFSSCIFAYSMNSIGNILKSINDSKLNYRRMISSINTYMQKNQVDHQVQGKVRNYIKYLNQREQDSTEDFESSISYLPKGLQAEMKEDIAQKIIQKIKILQQNFSHSTLNLLIQHLKIQNYAPGEYIYHQNEQQYCFCYINYGEVQINEEQSQTQIQVLKQNSSFNEYSFFTEQVTKSNALSIGFTQIVKINRKAFLSILKENQKDLEQFYNIKDTMLQYKDYSLLQKKCYYCGYIKHETIDCPLLTYQPNKMNVIRKYHKSSQLQPRKYIQRHYHYIPSRQQYQEIKNTISYAREYYISDLLMNDSQQFNKIQIIQKEEIPPESNLNEIISPSQKKDETEQTPQIISKSRLPSISTDANNLRRISKFQIPLNPNPEEKRQSKIQLDYFSINFSGLEIDTVQNYEKYMIHNNFINILKSFQKFKRKTKITNRKQKLMESFNNED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQA2</accession>
    <name>A0A8S1KQA2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal RNA-processing protein 12-like conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQA2"/>
    <dbReference type="OMA" id="PHISTIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052087">
        <property type="entry name" value="RRP12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287:SF1">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="888"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1005"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="895"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1020"/>
            <end position="1034"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1105" mass="127506" checksum="3A548D7ED3072401" modified="2022-10-12" version="1">MQVETFKDQLQAKLRQKSSPQNEKGLILLQSIENNLNLEQRTNPTAYIAQLINLLKSTKEGQDVIILLIYKILPSVPIVVVRKLIPALLEEIGKAQLDNLSVGVFGQILILSSKEDLNSPKMHQLFNLLLQQTISDKKKLRKQAIKTLQSFYQQKALDIQQIPEINERLKTHLKINLQENNECAQHSLTLLASLNNLIPYSILVDALYVIIEEIQNMDSKYYPFIFLSLEQVLQQNVGADQAEKLIQQLNQIQPEEVNAQHAYIQCIRQCLITLSQSSLSLAASYLPSTLSNMAELLLQNNQKLMQHCRLEMMTLIEKIGKATLSLKQDLAEDIMNLELESAGTLQQKYIATLTYLFSNRFDHANILDVFAKFFATIDDKTFEHCKEIIKEFVNNKSRWPHVYFEKTFGQLCNGVSMSNLLRTFPLKTEGVNPLDKDFDEKSNCWILYILSKYANPKLEEFLDFLFPLTNLLSLVSNANPSHEQKIIQNILFHVLEIASNSIATNDSQNHPKLTQFIELTLNYLKKEHVFFEKLLLMIAKLFSQLVTFPQLQNFAKQYISTLAKLVEQNNCTHTLNAIKVMSFAAPKQYIQEAFKKNMEKLLSSKDKLDDRALNNMDIVLQVVSAQQMTSDLWDYCINFTKEMINIPIIQKKIYKFLGAIIPKVHHTFLAEVYNLVLEAECEPSSRQKRFQVILQLEQYVISKLDSYQTFIQQFLPEMVVAIRDNNIKTRILAEKLIENIGQRLLGLDLLDQFISMVFAGLAAQSNVMKADAVATISLLVDKFYQNLKGEFIDEMTKLILLLLKEQNNEIFKSIILYVKVLIKHRHTLNSQLFTQLLNLDEVSRDKNIIILKRLLMKLSKAYPATAESLIPQEHIKLYKNAIKIEKQQKKKKNKREQEKELNKQKWQEKKQKQQKDSDLESDEESEQELGTKPKQQQGGMETETEVENNLLLKYDFKKEQFHFEDAIKQKKKIQEKEQQKQSTIKPLVEYKDGKVIVNEQKEVLGQKRRREAVNSDDEEHAQKKITRNDRKTKDVVHVVKESGSTFKSKQKAGGDVTVKGRPEPFAFIQLNPKALNKRFKNQASKAFEEVFSKKSGALKGVKAHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQN2</accession>
    <name>A0A8S1KQN2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230223</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQN2"/>
    <dbReference type="OMA" id="YSEDYGR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="615" mass="73118" checksum="0E8BA2D152E2C2B7" modified="2022-10-12" version="1">MKKSFTSHSPSSDHYYIGPREMKLPKFEFEYPPSCYGLVVSSTKNSNKTELISDSSSESLLPPLIYFKIKEKKLQKEQDEKCVLVGNTLHQMPKKIIKPKISYQPDIFPLKALKKKIVKQKFNGIYFEDERYSSDPEFQLNYANLRTYYNSINLSNNLYVQPMINSYKAYIGKGNNGQLVQWILKRRWWWSIVDKEKEQVNFLWQQLRNNSYIELIPESNQEILQQQVNNQEQNLIIQEIQYYNLLPNQLSFQLQKMRKKIGKNSKIISFDQALKFSQTLRLFEVNGNSKLHNHIQNNTHLGSKKNLFYNMKRYYEQQGLNVFDFLPQTYHIKNKEDLQNFVQQNQNQSIWIVKPAELTNRGHGIHIFQTINEVQQYLRSIHHHKNGAQKTFIVQKYIENPLLYNQRKFDIRCYILFTSVNGQHKGYWYKDGYIRTSSQEFSLMNLSNKLIHLTNDAVQKYSEDYGRFEKGNKVSFEEFNNFLPIDFYQTIYIQMRNIALDQFKATYGKLDPNRKENSFELFGLDFMIDDKFKVWLIETNTNPCLECSGPLLTKLIPQLIEDLFKLVLDPLYPPPQFYNSKKFIYDSFENKFELIFDSSMLDVPLKFTQNLIDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KU46</accession>
    <name>A0A8S1KU46_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KU46"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="54"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="201" mass="24256" checksum="B323D1BAD9F56420" modified="2022-10-12" version="1">MSICEICFIKDKLNICPKCSRNVCSFCIKLSDCDLCILIEMKFAFQYQQHNLQIEEQEYWLKQINEKLNTLNQEISLIQKANSLFNKNQNEKCQQLEFQMSQTIIDKQHELEDIETEIENQKVKYIYFNMILVYNSNEELNELKIYQLKLSTQNKELDLKINEAEKEYEVLQETLNQKKITNILNLINFEKQNQFNKRQFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUG6</accession>
    <name>A0A8S1KUG6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUG6"/>
    <dbReference type="OMA" id="YMENCAF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028008">
        <property type="entry name" value="DUF4441"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14536">
        <property type="entry name" value="DUF4441"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="206" mass="24916" checksum="B1B2C6089CA6FB96" modified="2022-10-12" version="1">MSLWDREDNQNNFKQIDQNYEVDQYSFNQNDNIPNYRTYISYEKENNTIQRFQTLMLKETNTKNIPKTFGNNFKKFMDNILMNQVQQNGTPIPQNMEMFLNKKNKGQQSTYNLKDFKALFNNEACSKWFQFYIQHCAFLDLIQSNRIENIEEYILFIEQYLTGAQNPEYFIKNRQQKKKEAAKQQKIQKKLDEMQLDNDSNIQLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUM0</accession>
    <name>A0A8S1KUM0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0250209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUM0"/>
    <dbReference type="OMA" id="PQEFEMA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1250" mass="149659" checksum="B8C76E447FDDDAF9" modified="2022-10-12" version="1">MYQDPQNNEEIILLQTIEKELSTDQQIQQICKIIGQQNQNPRLLIFLQELFKMLFNSQIQKDTLQKAFKVLNNILNKYKFRNRPIQTSDLSHYINQKYYDVLAHFYEKAFLKGSDEAFKHMNFIYYKKIARDYMQSSNCTNSAMTKSILTKIDQIYSTFTGNTPQEFEMAVFECLNMQEQELLTQIVTDDQSNTPKQTNSLQEKFILNLPPDLQEKYRSQNFTDIYTFKDYLVQEKQIKGIEKILGYIFVDKLNYYLNSYYFVNSAKKIKENKSDLQKQLLELEKDIMDSQKDYYSFIDIPSVKYFYKSLKSKRVRNLKGQCFSFKKKLIEFIKQEINSCEVPKKFNISQFEKWSEIYFSLYSELQRSTTIEEANIAFNIIQNVQQLILSFLRFRWNYLYLFCALIVEAKIEKEQLDTILDIAYDMMQNTNKANLNRSFDQILAQLNVKANTQITSDYFNKNGSNLGYNSLQILESLFPNINNKLIKVMLQIHQQSNQKIKLINDLKTFEFQLIHDCSQYLKNYLKIFEKLNDSILEGEKEEKEQKAIFFRTYAIQYQQRLKFLLSERSNKQLAQQFFNTYFLNLKKLKQDYQRKELGHYQEIFQREEKICQICEQYILKEAERQNQQGDIYKDVIPIFINNFVKDNPFVIFLPSLFKGLSFFKPQKQIFYEKLERVRLIAIAFKDKDQNEINKFIEFMIAQKDLYTFEQQFQIAKNIYNFYITLPSLVGESSLCKPITESFPIFSQMKSISDYNSTIYNAICVFTSKFFSLDQKAVIKSEDQFRNSDLLKKLSNLMNEKTLLEFNQQDSLSIQFQELKNYKAQLIKEVQQYNNQNFLNKCIDVFQRQSSKQLIISEIIEYKKQSKLPLNEIEQQQWSKNYWIQVGNQEIKQNNDNLIKKKVEEYFKQLIGLPSISQLNDKLSKDFNSFQDPYIKDVLKKQYPRYKEEFEKNFNRAVINPPQIKQSILQLTEIQKKLSIIRQDQKYKEFQIEDQSVLTFINEQFSLYMKQLLGKVFQVAITQKPGYEFKVFQNKSVSPNDNLMNKYKQNQELQGKYGPQKNSYLDIALLTSSENKNQLYYELCDYTPIQRQIEFRNFLIEIEPLQEEKQKNKIRKNKWKEEHPELEEVLRKKIKRGAELWNLVQDFLCKRENNENMMDSDEESEKYKGQQQQTIDIVEDDQLDQKIQDVQRNFERQLLTGRNYIKTRLNNMDNDNPLLTQKEINVKTVQFVLEQHPYFKKSKILYKSYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUT6</accession>
    <name>A0A8S1KUT6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUT6"/>
    <dbReference type="OMA" id="AYAQIEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="353" mass="40775" checksum="68A1B78C153580AC" modified="2022-10-12" version="1">MFLFIFLTLAYAQIECHKDDLVTTCSMNSFQAYMITYEKKTSDTMFLFIGAKPGQSSLFFDQLGVGPNSTYEKWTDLGHLMFLDLQGSGYTITTKTPKSIAEYTQQCASAFNTFKDQLDYKFTRLYIVTSGIASKVALELALQLQAYGTILFSPLLNLLENMRQLPSTAYHMGLIDFQERQSLELQVMSLSQPKYSDYQILVNKVSKMSGDMLVSDFRKYELDNSTIILTDIMNTQVVKDKYHAKQQFFKTFNIDIFNNMTDSLSQNIDLEKYLKQTKIICLIPQFSLTTNIQGTLSYLMQYKHFNEQPFLQNVNDNQYAIYVNIGTIYIIYNSGDKIYQDQQDIVKKIIRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KW22</accession>
    <name>A0A8S1KW22_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0230071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KW22"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026205">
        <property type="entry name" value="PIBF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18950:SF0">
        <property type="entry name" value="PROGESTERONE IMMUNOMODULATORY BINDING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18950">
        <property type="entry name" value="PROGESTERONE-INDUCED BLOCKING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="321" mass="38152" checksum="42AD4CC516872E53" modified="2022-10-12" version="1">MSTKRKDVMNLMKSMNIDPSTFSEEDIKQFEQDQVMMNDSESDSMRDSNRHNKGGNIHDTVNMKHLFQLILFQNYSSVVSLQEKQISFLNAEIKRVQAANESDRQRIFANHRIETDTLLSEIKQLKSKMINQESQQPGIREALAQVRDILTGLVPKAVYLRLRNMNEKKMPIQDWVLVQVQEIVYPFKKEGEFQKKEIMALREELRQITEKHQFLLKDLEHGQKMMVDREEDIRRHKLNYDNTRKALELELIKSQEDIDPQSAILKKKYLSKQSIKRLRRTFLVFKFNAKTEQMTSYLVFTNQEDNHYQNQILILTFCNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWX5</accession>
    <name>A0A8S1KWX5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0260267</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWX5"/>
    <dbReference type="OMA" id="DRFFHKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05353">
        <property type="entry name" value="hydroxyacyl-CoA-like_DH_SDR_c-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051687">
        <property type="entry name" value="Peroxisomal_Beta-Oxidation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024:SF2">
        <property type="entry name" value="SCP2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="3">
        <location>
            <begin position="9"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8057882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="310" mass="33698" checksum="DA7E30A73AACE797" modified="2022-10-12" version="1">MSDLRFDNKVVVITGAGRGLGKAYALFYGSVGAKVLVNDNGCDLDGKNTNPKFADEVVATIKSKGGIAVANYDSVLNGDKIIAHAIKEFGRLDVLINNAGILRDKILAKLTEEDWDIVIKTHLYGTYSVTKAAWPIMRDQGFGRIVNTSSGSGLYGTLGQTNYAAAKAGIVGLTLTLAKEGERRNILCNVLVPVGASRMTETIMPPDVMSGIDPNNLTPILAILTHESNKEYNGGIYECSGGFFSRVRWQRSEGVSLDLPVKISEVQKNWAKINDFNGKNEYPTGNADLFNKVQENLERLEQQAAQKPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KX12</accession>
    <name>A0A8S1KX12_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0260292</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KX12"/>
    <dbReference type="OMA" id="ANAEFYH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="279" mass="32563" checksum="BE88B442B0FF2076" modified="2022-10-12" version="1">MQKTTLVNFLHNGSNYYPFGDKPEKFCSTQGRFVPFIFERNQVGKLANAEFYHFNSNQKSVKHRTSEQANSLLADTIIKSKLHTEQDTPKYDPITKYVTLIPTSQKIDELHKTIDIKTYTLNHTSVERGQQMRKQQKGLNHKHQLISRSTKTLTSQIPAIGIYDPNPLNRHLPNIQIKSKSIQKLPEVWESTTQITTPKINIKNSESQMMFAKQIAQINREVDSQMKRNPNQKFSTLQFDVHENMNLEEAEQMRNRMKQYFQSVKDSLTKIKNLINIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXP9</accession>
    <name>A0A8S1KXP9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0300040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXP9"/>
    <dbReference type="OMA" id="YITTRIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="491" mass="57668" checksum="C7DE6521037BA200" modified="2022-10-12" version="1">MHIQEGAIETFYYYLWLQNDSIKQNKVEFHLPDTLIYRLGVIQAWYFTHNNEILVKKLENRTLPNIETAFLKDCENDVCAYFISCKPRRDFYNTQETRGEFLQDQLNIDCKTSILYLNQEQFKQFIHNPNIHRNGILQKFVKPALPKENVIQAIWSQNLLLLSQRVNNIELNSSKFDAQEKCSTFNSPESYSKAYPVKSKVITTQIQRLINYITTRICVVTYEKFRVNRAVLYCKIDHLQRINILYCTSLRSNDECPFPIQFGHQIRPDNVKNTITTNKQKPIKLIMDVQCLSCQQLCQTEDLHPIKYQKLIASGQHYRMDIKSYSTRVQRADHQKTEDNPENYIANTTKLIPDIIKKLFPSMSYSEFAEVKENSAFLNKELLVCFNCYLKFTQSKQRLNQISRQLQLKQKNRSITDLIRQASQEVSQRHTERDHSGNKEYQKMTLCLTQRSTLLKSSEAMTMKSTMKSSRTSSMMKNNITLRSTARKSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZH0</accession>
    <name>A0A8S1KZH0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0300089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZH0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="35704" checksum="E440F43782C931C5" modified="2022-10-12" version="1">MNKFIQDIQTQFYKNIKITFILSKYLEKFQSKLFLQYIIQFKILKSQYFNRALDHSTNLFETENNKLNVKFAQNERAINYIAIIEMNVNYVALFDTKVINTLIMLQQGFLNQKLNLFVYQMFFCFEWKLFRIIKQIKQPYSIDIQLEYIQQKIKQVIYHDPQQKWIKQAFAIINCVKFQMFDINLIQFILIMQDQRDQLILDIKIANYRKQKQQKIEQEQQFKEQLPKIKQHKAQIFQDDMHHFAKLLFTGFPIAMGSVIIFRSLIIFPFAIWTTNYMQNNMHKLYEYLENNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2P8</accession>
    <name>A0A8S1L2P8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Parkin co-regulated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0250118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2P8"/>
    <dbReference type="OMA" id="IGDRIDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019399">
        <property type="entry name" value="Parkin_co-regulated_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207:SF2">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN PARK2 COREGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10274">
        <property type="entry name" value="ParcG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8056914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="33396" checksum="2EFE663632EC83EF" modified="2022-10-12" version="1">MLNYYQKNMHTCIIRLDNQFNIKFRQVCYNNNKQIKEQKDILHKDHQQQICRDRWKQQYISYITFLIVCSSLAPKKSQAPQKVPKSGIFQALPMIEQTLFKKYYDRGDLPIAVNFSGAVRKIVWKCEPECLDYHLYLPIFFEGLREVEDPYKFLAVNGCEELLQKGETKILSVLPQLIIPIKKALATKNHDIMCITLKKIQKLVKSGQMIGEALVPYYRQILPVMNMYKNKRLNIGDKIDYSQRKNENLSDLIQETLETLEKNGGEDAYINIKYMIPTYESCMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3A9</accession>
    <name>A0A8S1L3A9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0290296</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L3A9"/>
    <dbReference type="OMA" id="QNRTHFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="225"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="639"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="800" mass="93696" checksum="1DB7A9795D437B10" modified="2022-10-12" version="1">MSSFYNHQPRRKHSQTSFYLKTLHCQSSPIKDYNKDNTQQEYLQNMLRMQQKEIERLEHQNRNLQILTEHQSKIIYQERQNRTHFIPKIQDKDTMSSQQIINKQKIENQLDKPIRIQRKNTINRKLTVLFQQDDTFKLNSLLDLNITDEELINNYQSDGSLSLIEETLNDEDCFLDVIHNYPPQKVSIIYDKLKKLYHEHNLMFHIILKLKLLIQKGFMIQNSKLLDESFQILRESIITIMNSQEVKIYLIDEEKKELYSRQDNQKYQIGEGLIGFVAETKQILNLKKAKYDTRFAPIDSNVILAAPIMDNQKCYGVIVCQEKKNGGIYSEEDESLLQLLSEQGKMILCNVMNHNQLMTAVNKFRHALKSSIQFIQLKSQTQLIQHAQKRLKEMMNSEIATIKLIENIEGQGIMGEAILNKSITYHHNSYNSQSFNPNIDLNTSLPIFTLPVICNKQIVACAQFTQVRGLVQQSSLSSIEQEVLELFLQSFGYLIKAYERRQKQSDGEQEILQTEQRIHKIGSIKNNKMNYDKDEQFFSKEDIPQRALQIKETVQTFVDPDILGLRKKEWNSSVSVPKNPLSEETHERKLVKIRLGLFDHPIPKEKANKIYEGIETRDNYLLAKKGSSKWNVSNETNNLEFQKQLINQTKEALKNTKTKEEEIIKNYLKPIEHQTKIQLDLRSQKVNEKEIRDKIRQDYKLANPAASQQAIDGAVFRLAYETKLSKNQEQIQDKNYTFKPDMARTLKQDLDYKYCHNGVWQKMPDGSEGWSCCMNSTLESKGCITIKIDKNKWDYSSFTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L8X3</accession>
    <name>A0A8S1L8X3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0360207</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="MMASNEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035926427" description="H-type lectin domain-containing protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="1838"/>
        </location>
    </feature>
    <feature type="domain" description="H-type lectin" evidence="4">
        <location>
            <begin position="35"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="331"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="397"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="800"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="875"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1226"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1260"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1402"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1455"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1758"/>
            <end position="1806"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="679"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1505"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="398"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="800"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="883"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1226"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1280"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1301"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1342"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1412"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1475"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1769"/>
            <end position="1784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8064600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1838" mass="213877" checksum="C35CDF68DCBBA7E0" modified="2022-10-12" version="1">MVKQCLLLITFMVIVHSLNYGTILIQNEKGGQIQTKEIFFQQPFQQIPQVAIALLNGHGDIFAEIHNINQEGFTLQMMASNEIEAEFAYLAIENDENYQIECFNRIDVGNEIQFPLIIQRPTFIGVLATNTHANNFNIDLLDGNLIVDNDGQLNMCIIITNEEQPIELIGYQNYQEQEVAEFHSFKKQNAMSLVQAQQSLEITHLDQHKSFEIWHHQSIEIIKDSSNEVNQTHKSIEIVNEDQPNQTQFKQQESIEIFKLDDQESQSNIDQKLIAEDINLDNIDNNLVSNNNQLEDIKLEDSSNVDQLTQQQITEDASNQLEDITLDIESEQQNQQSQTINLDDQQQSIESSQQQLENNQQEQIEDVKLVTEELTNNEDSQEIKLTTDEVQIIEPKNESLQNSEEQQQVIDNQQDDQVQTEQTEQIDQQNQDQDISNLSSEVVINNNDQVLQQEDVTFDSFDNDNVNQQEESQQEIQIEDIKLDDTTISSINAEDIPQQQDAIDIQNDQLSTQSDVQPIEDTTSTDDVVVHADEVKIENVKISEANLDDISTDQQVQEDNTEQIQQDNTEQIVQDNTAQIVQDNTEQIQQDNTEQLQQDNTEQIQQDNTEQILNDKQDNTEQIQQNNTEQIQEEIQENNINSEIKELSEDISVDPDAFSEPQQKVTQINDDSLKKEQDIDEMIKFAQELKEEAKQLKNKSRNERQQAKLDIMEQANNLEKEVGKLENESEEPKVVEDQISLDVNSAYDDQKDIQIEQPQEEQQPPIIYLNDSGVNPEQQLITQDIKDDDKLMISENSLQEQVDQEMQSQEQQVQTTSQIGEEDIQIIDASIKLDEDHQELEIYQSGQVEVIPEQVQPEVEVIPEQVQPQVEEVQQNEQPQEQVEVEPQEDYVQEQETTKEEGAIEIGLTTDEGEAFIRKPKKSDFLREKQRLKDKLNNVIKDFKLDSVDSNTLDSVVEGDKQYSLIIDRLDVPENNEQVIVLKDEDYEQSQIEPEPEKEVKQLGDYLEVVEPNIENVNFIYDDEQQQEQQTIQIEDIKLNDDNTDEETNYKIVEAKDDDLVDTTLESIDDSQQYQLLSTDVAVTAEETSTANVENQSEDEINISFDEFQKELEILANTIGNIKQENVDTKQNNDYIVVNDNSQIYEATYDEQSNDLPQEDEPQIQEDQQIYYQGFEDDQNNSIEIEQPENQDEQLQEQQEVEQAFDVPEQENNQEQLSEDQFLVNEQSTEDSAQQVETYDDAEPQDQYLTIDNLVKDSQAIGESYDQAPQYYDESDNESNEQQNQNVVESSLESDYDISEQEQQNQEIQEQQKSDNGQQQQQQQQQQEQDFVVMKADQFKPQLQDEIQQQEQQSDIQQVDDIYSETPKDENEIDIIVDVGNQQDDQPQLSEQDYDYGDNFEIQQPAKNDAEQQSQQQQQQQQQQQQQQQQQQEQQQQQQQQQQQQQQQQQQQQLQQDSISSKKHKQKIQHSITISHNPQESNNKQTDRSIEIQHEDKSLKIEHPVNQEELKKDLKEQLKQELKEELKQEIKQEIQESHNRRKSPSLVIEENVAKDPERPAQKAINNFNNLLNDVVDETLKEEDKKHYQKLPIVDVKKESFNKVEPKIEQGIEDLKKALGLKGGVEVVDNNGKKQVINVDDLKKESEKLLTRPERDNKNDSSQYEEFLKKVTEKSQTNNNDQFKNYEEQHNIRKQEILENIKRELEIKKGHFTVPNDSPQAELSPYEFERVYLDWEKNKQDIIPSMLQHSIEQPITQEMHSNSEVVQQKSSERVSRKEEVEKEVQELLYGNQKNRKQQSKQSSKEFKVEDLKYIKDDPLLDATYSGFLQVKANLRRRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBP3</accession>
    <name>A0A8S1LBP3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0340049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8062903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LBP3"/>
    <dbReference type="OMA" id="HDQDYQP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="141" mass="16689" checksum="17BC3A4781F55B75" modified="2022-10-12" version="1">MEFENDSSQAQSTSQFQNPLFYTPSHNQQVYTVPPNTINHVQYQNSQNQFWIQGTNLINQQQMIVQKTLIEQPSLQLQTQQFFQQPQELNDDDHDQDYQPEEEQGSDPTSSDEQQEDLQEEEQFDDPDFDLQAYLKVRDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LD35</accession>
    <name>A0A8S1LD35_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0360049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LD35"/>
    <dbReference type="OMA" id="ICRIWDF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045913">
        <property type="entry name" value="TBC20/Gyp8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913:SF7">
        <property type="entry name" value="RE60063P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 20/GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="32"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8064285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="356" mass="42285" checksum="96AE533E0AE24326" modified="2022-10-12" version="1">MDNSQNLLSLLHNINHDVGYEKIRKYGRTNGFHNIYFRQQIWSKLLGVDQQLPYYEREKCLQHDQLLKDVNRSLNTISQIKNFKEIDQLRKCLMEVLDTIFSQHPNYSYYQGYHDIASIFILVLGVEQGYIAATYAAQNFFKDYLDQELSTTITPQYLFIRNLLLRYSRDTSNIKLRQLMLNIEHPTCVIPWILTWFSHSLDNINDICRIWDFLLCSKQNTILYLCASFIAIHHQVLDMRNEDDLIGEFQEFFQNLNNKDRIQDLKLEQVLQLTKTLETQYGFELICEQEHIQFSQNSIVYQHNFKSQLPYYNSLAQLEKSIPKKVFNFINDNQDIILNLGGIALSYLIQFYIVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LG13</accession>
    <name>A0A8S1LG13_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0380092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LG13"/>
    <dbReference type="OMA" id="DQMERCL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="301"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="371"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="628" mass="74356" checksum="EE9DE708E2772750" modified="2022-10-12" version="1">MLHLEIEVQSLQFFIEKQSEQCKIIILDENETKILMSKILSQCSSVQDINEVVEFDFNYTENRYLKIMVQDCKICYGEAILNLGFYIENNLPLVIDKIPIHNRYDQEAYIQVGLAWNQLNQTEYKRLVNNKSKMNMDIKKSEEWLKQKRECQHSPIQQRGENLNHNREETLKKYQEQPCNFEKLLKYSEIDKLNDADINAKRVQTVRVQKVCLSQQKSKEEKFDLDCPTVLRENKFVIPGKNRTKIVQNQEEILRGSTKPKANPKKIEQFDNFNSRPTQNEQALLLSCRQLEVGEQDDNFIQIQNQALKNQNKQTKQQNEKLQEQIKKSEAAFNLLSETYIALQQDYKRIQIQNFNSNISNSLSDKNSKEFQQIKKESEILKKEIEILLRENNILKDEKDQQKKQYINEISKLNEELSKYKNILYELQNEKNQLQNYIQSQKDSQINQYCYICLQKDELQGNKDKIQYKTSICQENIQQINDEKGKAKTSKYQIDNLLKNEGKQKLNQLSEQELQNLVFEYSNELQIILKENQQLSKDLIYFQVLNSKKDQIRLESYQKELEITKRIVKLSDQRCSSLEHQIIQLEKAVLNGKQNIADMINAVMECGGQKLYDQMERCLAFRRSLKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKI7</accession>
    <name>A0A8S1LKI7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0400054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8066985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LKI7"/>
    <dbReference type="OMA" id="NIPLEPC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="74157" checksum="D49075B22D08B977" modified="2022-10-12" version="1">MLKFQKPQTCSKNLTTQVIERFDFFGLLPQFRILKRQKFSTPLGQIITIIICLAYLYYIIHEILDLTNRDSPKVVFSELQPTNTSPLYFGKNNFTFVLTIANPQLSSLQTLNKYFTLSVQNCLRIRSLNEDGISQIDYNCSNIPLEPCQMDKHFQEQYQQEYFEKFKLQYMYCPSIDYWNQYALQLQGYSQEDRFQFLTVTFSICKNSTDYQGCASLEEINSKMQSGFFVMYLSDALIQMQSPQKPYQDVISIQYTQFSLTNSKSIHSTFKVTETKTDYGILKTDYVIDTAIMQSTIKEDSTPYNQQYLVQNSIFLEQRRNQYQRSYLKLYTLLGQIGGLWQITIIFFNCLFYPILFSSMNLAMANKIFRFESKESLELDILSVAKSVPIGNQLNKSDVKDFQDDVMKSENIQIRKSFAEFSKTQQEIKKFLRKKKCSLNFTLLDNIKFQLGCKKDKQRQLKYAMNKIINKLDICQLITKFNELDKLKHILLNKDQLALFNYIPKPMIPYDMFDDNFEKKIKELEQKQEYKFILENEKPDVLRLDDAYEAYSRVIKKQELTSTDEAILQFMDDDIKRLFSRIYSNQLEMLCQNLQSSQVFMKNNTERVDIQDEPHIQLVFGSITSRQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LL31</accession>
    <name>A0A8S1LL31_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0420143</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LL31"/>
    <dbReference type="OMA" id="CDEEVHN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="261"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="84"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="262"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8068407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="293" mass="34912" checksum="AB783319027DCE10" modified="2022-10-12" version="1">MKKLFKYCDQCSIKPGTVFCQNCQARFCYDCDGEVHNQNLNHKTEICSLTQMRQINQYSQIQTIDSQIFSQTKQNHVVIAQQGIQLQKEELTKIELEIKQFEQVLNLQENKWKKQFEILEKEYSEKIAKFEQKVQKSESTITEIKQQSKQEDQINEMMKQLKEQLEGQKNQLLQKVDQQKEQLNDKEQLLQNLIKQEKDLSAEINKKEILIGKFKEFIQQQQKEKELILNENERIKKQLTEIKQLFKEKLPQLGLDENFFNFDEEVSDAQEEEQEPQIQQNVEDSTEEDEGIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LT75</accession>
    <name>A0A8S1LT75_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WIBG Mago-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0460123</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LT75"/>
    <dbReference type="OMA" id="QDEPNTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015362">
        <property type="entry name" value="WIBG_mago-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09282">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01273">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WIBG Mago-binding" evidence="2">
        <location>
            <begin position="122"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8070757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="162" mass="19319" checksum="E2FD72BF89F3FE14" modified="2022-10-12" version="1">MSEVQELIDNFEKIAIKDNNDKEDKNNQKIEQLNKQDEPNTIIMKESNNEQQQQQQQIQENQTQKESQIDQNNQKDLNNNQFNEKELEEAFINKLVIQEQNKEEKQQQQDQTKIDDQVQTQEHTIIKGSMRSDGTMRKDIKVRAGYKNADMIQRYVIPQKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTD1</accession>
    <name>A0A8S1LTD1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0450183</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTD1"/>
    <dbReference type="OMA" id="WGDKYLY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000048">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="352"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="610"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="79"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="113"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8070349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="743" mass="87082" checksum="554C2032B8BAB98E" modified="2022-10-12" version="1">MPPKKQHLKTQSSELSNQVLMNYQSNREALNIRMSNSPIFQIECKSKQIGLHQSTIFKSNASQPNLKITLQQQTQRKIQQQDILKSKGFMSPKITSQERVNHKTQPKSRGNHMKTQPQTYSNQNSQTKMKSVESIQDDSPKQLFKNQQYSPSPKPEQSFKQQTSETKQKQSSDLLARLNFQTSFIVSSVKNQTNLFSGPQIYQLQNHQKSNSAQFIETQKLYTQSSSQPRIEIQTEKIKTITTRKFQQDFIQIILQFKSLKQKMTIDISTNKIQYLVELIKEELNKKFVDNDSLQIIGIKTYNISIPIDYILSSIDRPLSLLSNCPNQPLIIEPILQTEQENRLSRVCLRDFEFIRCIGMGGFSKVYMVREKRSGQFYAMKLIEKISIISQNKQNIIQNERDLMSNLNHPFIVKIQYAFESRRYLVFVLEYCSGGELFFLLRKVKRMNEEQAFFYFAEICLGMKHLHDKSIIYRDIKPENILIDFDGHVRIADFGLSKQLDQEIAYSFCGSPEYMAPEMLLKQGHNLQLDLYCLGALLYELITGLPPFYSRNTDEIYQRILNQKLSYPPQVQMSSLLKDLLNGLLAKNPKNRIDRIETLLRHPWMNQWGDKYLYKELLSKKIDPPFKPNYYSFNFDEEEFGQGDAEFLQQIKTLQQNIMENFPKETILKNFYYNPKEINCAESTRGTNLSTKLLEEQQVTQRQKSKRFNTYEDDISNYVNEQKILLLNQLRLQTENEIKRKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTN8</accession>
    <name>A0A8S1LTN8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0430157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTN8"/>
    <dbReference type="OMA" id="QGIEINC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000298">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14248">
        <property type="entry name" value="CYCLIN Y, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="622" mass="72889" checksum="42390E28914895E0" modified="2022-10-12" version="1">MKKWDDDARLKSYVNKILNVKSTMQNKLNTASKKRINRDTEYSSNATHRSDQTSFKDSILYKQLVEYNLQQYTNKLLLRGYKSGLQQLAQQSQDSQNIVLQEIKLLPGHKQKFIDLFKQLNEHQESGNDNKIVNYNSFAQNRNTLPSQLNSHGQFEQTKEVYMEILKPINRRASMKHFSPSHEQTQIKPRVLPKLEKVQSKGKKERSPQFDEINTQQIDLQVNNVAQILNTKESISKPTSKNIKNPISNQKNQTQSLQKTHQDKPNKFINKLLLINGKNNNEVHLERESIQLMYQSFDNGRLASTLINIDIEEISYCVGISIQKMMVQADLDNIQNINNNLIQQVDDNVSSQSESQNIINDENDGDYFQDQNFLTQQDVNEKKSVKQEKLYQDEELDYLEDLENNEQQNNNPQSEYLDQQFQPDDENNQQLPKSQFSVDTTLNQQESFLFNKVFIDMQLTNYIPNVDIIQNYCKNIMTTTKMEREVAIISMIYINRLLEHNQGLEINCLNWQKMLFTALVMASKIWDDESFENNNFAKVLPQFSTIQINEMEKVFLKLIEYHLYVNSGDYARQYFLLRTYADKKQRSYAVKQLDIATVLRLQRGGQQLITKQQFLNTQNKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTW7</accession>
    <name>A0A8S1LTW7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0380199</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTW7"/>
    <dbReference type="OMA" id="HLMIMVH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000023">
        <property type="entry name" value="Dynamin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001311">
        <property type="entry name" value="Dynamin-like protein-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF233">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_59, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="4">
        <location>
            <begin position="27"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="3">
        <location>
            <begin position="531"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8065964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="625" mass="72362" checksum="510832A9FEE5B507" modified="2022-10-12" version="1">MKSGLWDNMRKMVTLIDDLRDCGVQDYIKLPRICVVGMQSAGKSSLLENICGLDFLPRGDGVVTRRPLELRLVHIQERLSPWAIFKEVPEKKFTDFTKVREEIDRLTDAKAGQRKNILPDPIELTIWSPDCPDLTIIDLPGITLIPLRDSDQPDNIEEITLNMCRRYCEDERTIILCVMPASQDITTQRSLKLARQLDPDGIRTIGVITKIDIMGEGSDASKVLLNEEVKLRLGYIGVKNRSQQDIINKVPVQKALRQEEDYFQNDPKYAGIPQDILGTRALIEKLTTVLYQHIRKCLPDIIKEIDTKSDECEDKLKLLGTELPRDQKDKVQLVFNLLQDYTDGFKNTMDGKYDKNEATITDEEKKIELTGGAKIKQMFNELYQEFADEKYHVTDCYLDKDIELADQLHQGDSIPGFPSFDSFLYLIVPQVQALIRPAQDVLDQIFQYMDEISQQILRKVFTRFPSVLDEISEMSRKVIESQRAKAEQVVINQIEAEMNYIYTNDEEYLTTKADLVFKQNSKTIDPKKILVRELRNRVDLYYKILIRNLRDSIPKYIGYYLVKQTLDKMQLVLYDQMNKSDNVFQMLEEPVYILQERQQLQKTLKALKQAKKVLKQDPDLSKGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUJ2</accession>
    <name>A0A8S1LUJ2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0440155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUJ2"/>
    <dbReference type="OMA" id="EYDRTIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033362">
        <property type="entry name" value="SSNA1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28661:SF1">
        <property type="entry name" value="MICROTUBULE NUCLEATION FACTOR SSNA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28661">
        <property type="entry name" value="SJOEGREN SYNDROME NUCLEAR AUTOANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="41"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8069685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="131" mass="15040" checksum="36CECB25682D35F0" modified="2022-10-12" version="1">MFTQPSGAKSSAQSTPYKTKYHHPEQEETISEDESQINNILKALKQQYLILDNTLNEKEEEKSQILEDLQILNQRLQQLNKSIAIKRQKYEKCDRTLKEAESAFATIADSTKSLLQIVKSNIGDISKKQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYU4</accession>
    <name>A0A8S1LYU4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0470198</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYU4"/>
    <dbReference type="OMA" id="TKNCCIL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8071472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="568" mass="68548" checksum="D23BD3569731296A" modified="2022-10-12" version="1">MSKTPNRIILPELENKSFRDTKQIQDLRRKLEQQQDTIKELVNLVKESKTESYKPSFNQTIIQPKPEKDEVTLQLLEEVKTLRKQINNIEQGPKQSVQHPIIIPQYLPGPPQQQQQPMISPYMFMNPYFPMPPPFMYPYPQQSNNDDPYKKIVLELLQKNNKINRDLNYKIRDSYSSEYTDDLYSRNNSRQHRHHSNVDRRKHHTNDRYNQRKNRDKKQIKDRNQNKIRDQDDSDHHRTISRTSMNDSQMSYNSNQNGKNKKKNNQKIFTEEEKLKLRRKLIGLFWYIRIGLVLRKYLKRVWLQRRQEYYETEAQQLIDKFDHEFNYKEVFILFVVECNKNKYFTKNWSIFDNKEVELKSKLIFSIITALFKKIPFMTKNSMTDEHRQFIKKISTSGGYLLPGHPQFVTDRVELRPNVTIGQINAEVSKLIQMDYVYIQILVQRVILIYEWYSQFSKIPNYKEAMKIFVTLLHQLFIDHFINLPTFENPDQIFNQQQIVYCDFNQQNYIDVAISIDSKHQKYQTTKNCCILGLGSYEEMRPLYEQPGYKDIQIQFKEYCEYFYQSLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0R9</accession>
    <name>A0A8S1M0R9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0480244</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0R9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="227" mass="27590" checksum="B6C28C89619273FD" modified="2022-10-12" version="1">MIIFEIYIKENLQELLSLDLSIEQIYDYCIRVYLKEIKKPGTTILKTYNNIIETHRYQQLYFLKEPSSYQYHYNCLDLEQDESNKYLLLSSLYNRNRFEEPRFIYVSDLLRRRMQQMIKDIQDRNLTIQKSINLYYHQNKKKQYCLTPKPMRSRNNTFYKNNVKLTGLRVQTEFVIEKRLPARSIYQIKKIQLFDENENSTPNNKSSCIALGRPRSHIRTKFQQLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4V6</accession>
    <name>A0A8S1M4V6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0510213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4V6"/>
    <dbReference type="OMA" id="HQQNCKA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="110"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8073732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="153" mass="18062" checksum="A04194519AE78355" modified="2022-10-12" version="1">MNQTQIDDMRLYPCSNCGDHYPYCFISDHLESCLQENLQVDCRYCGETILEKFMQDHQQNCKAYALQETEDDVCEFCQEKIFKKFKQDHYQDCPLKQVADAYQSYKAQECPICLIEIGPADQKGLLQCCHVFHQECLQQWQKKSLECPVCRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDJ5</accession>
    <name>A0A8S1MDJ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0560068</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDJ5"/>
    <dbReference type="OMA" id="CMVFNLV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="111" mass="12738" checksum="48050906220A64D4" modified="2022-10-12" version="1">MNNLNKNLKTDFKIVTNMKLYFQNNIEQLIQPSEDEPQLEITSNSNYVLLPDLKSMVFNLVDLERNDFDQQSINQSMYMLSSTNASCNKISVIFQSLSNESISSNSIGEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLD7</accession>
    <name>A0A8S1MLD7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0550040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLD7"/>
    <dbReference type="OMA" id="THWSHAL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="16242" checksum="3C877FA60476F9B1" modified="2022-10-12" version="1">MQEWPKKLFLAIAFISCFTCYARPDYNLPLFAFAYLLWDIDRPVSQKIRLIYLFVYSWIIDFVWLVYWGPFWNSSTFSHNWADGIQTFVLVLSIINFIIKLGTIVVCILAEKECKDALHPENAMAHAKNIFNSEVQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MM05</accession>
    <name>A0A8S1MM05_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0590028</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MM05"/>
    <dbReference type="OMA" id="IMSQRNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="311" mass="36057" checksum="B1F205A4E3A77EC7" modified="2022-10-12" version="1">MLRFIGIKINNAQTLIQGTIAYNQSIVSQIPIPNLLIVTAHQIVQKRNNKLQKLQKLPVNNVAATNNYRILFNTTQNIDLSSEIEEYENKIISWETSEHNIYTNSNVSSQKMKPVIKRQMNYLNTSNSINQLYYQQNDKDISMIIKDINKFTNRQLMKLSPKKMLINKQNDKINGNQFNNKNVIIPKLLIPQISHNKINFQQLLNQYDQIMSQRNCQTERQQKGKSKEKSKVQLDVYQIKNKQQQSIYSVPSSSNFQGKGISTCSASILLKSLSKKSMDKSPMASRKLEIKQNLTKLINQQERDLGFYTQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MR90</accession>
    <name>A0A8S1MR90_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0610229</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MR90"/>
    <dbReference type="OMA" id="SHIIGHM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035766405" description="Fatty acid hydroxylase domain-containing protein" evidence="6">
        <location>
            <begin position="17"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="7">
        <location>
            <begin position="135"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8079265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="283" mass="33461" checksum="6B2EA30DF141D3F7" modified="2022-10-12" version="1">MIKLLLLMTSIISSYCFNPNDYITIPFLRFPDSLSYNVLEINSIFEYLIACVYIGLFYYSMYFCISQLLVWSHPAPKTPERLAQTKEEMRRGFKALFYVILLTTAIIWKIQPLNPYYGYYETHEFGFKEFFISLIVYMVTFDVWFYTSHIIGHMPFFWKHFHGEHHEFVEPGAYAQDAVHPVEALIQGPIGHFLPTFIYPFHPACHHIFGLLTSIYAQLAHDGRWDPAGHTLHHYYYSCNFSIWGLCDFIFGTGYNQEKYPIPYIPTWLRSKVNNKNQDKKIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTJ4</accession>
    <name>A0A8S1MTJ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0700081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTJ4"/>
    <dbReference type="OMA" id="IRNDSTM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="419"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="909" mass="108906" checksum="04E96EEC0C321161" modified="2022-10-12" version="1">MEQQEKFFQEFKHIYSIDIPIDKSELTKENQKQKFLFYIDLTNDETYSKALAQLSTNIFIKKYWQGGILSEFQIKNRENKQSIFCYYFNKLIQQDEQINIQNLFRFRILLQEFISTQQNYQIKFVFHFRQKQLNQKDFEDYIIKCIYQILEGSFDVLNDIIFLYNQQDSEIINYCKLSKKKIDFQKSKQHGKFDSKFIETLDQFLNYFQFKKNDPNLIGYKCQNYVQDKVNVVLKVFEKELIQLQKDISLMIEFPVNLNNEIERNDNVKQYLESLKTIIGEIYMILKDIYEFDEKVKQIHIVLNKFRNQLIKYLSDIVKENSEFQKYLKLIYKDESPDDVFNQLLQFKFELVGSDNEVLQKLKKYNYNQDSINATLMRVIIDIKFWFEQFNLYLPQSLAYLKRMIIPQNQKINQDKPHVFVLGMTRVGKSTLLNILKNPENIIVNQNKNFDVKVKDNNIVISDSNNSETFLLQNQEIDNFIFVDSPGLRDTSNNNRVINHYKTFIEVIKAKQQIFLLMIDGEELIKNKNDLIDSIETINLMLGKQNNDNCLENLILPVFTKIRNDSTMEKIKIKWQNDTMKDIQDQFQLKILNIIKKAIDDGNYFIIYIAENYEIEFQKKQLDSEIKTLIEKFVEIDVENQEELKKLQEQTKSKQQELKKLQQVNFLQKIKQIKDDLLKKCEKILIARQKNGQSLNFEFNLTPELKDSLKKLLPHRKKFYQHIFNIIQSDFIAKMLYDDTKSINDKFKQMYDISQNEIKLFSDIPQNIITQDIQQYIEQLNTFNNIISQSDDQNQYLQEDDFQQFYNRLTLLASAFSKIDIICKAAGLKQIIWNKLSIKYWKNQSINKKAFFSSKILSEGLLIVYLQLSVLNLLFTNNVFVTEKEKEKLERLNLLKFQQEYWRKFEKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWM5</accession>
    <name>A0A8S1MWM5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0660199</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWM5"/>
    <dbReference type="OMA" id="DNQFKLK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="3">
        <location>
            <begin position="77"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="103"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="139"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="175"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="212"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="248"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="321"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8081846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="435" mass="50379" checksum="752FBD2F6B2639CF" modified="2022-10-12" version="1">MNQDPNEKDMIGDLLDDSFENVTKIKKSFTSNRPTTLQINQNHNQSPIYYPKVYSPQVKQKQNSYDLDLRLTKVLVQQSAPTSALIPKSYDYVPQFNLDDETSLEGDLISKCKDQNGARNIQKAFQEGSKTIREQIFNKLEKGLLSLSKDVFGNYVIQNLLEYGTLIQQQKILSILQPHSSQLAFHQYGCRVLQKLLQNAHNTTDFPILFDTIKNKVRDLIIDQHGNHVVQKLIQLMESDISLWVLDGIEGQINKLVINSFGCRIIQKAVSISNNHPDRQMKILIEIMRLAYELIISQYGNYIIQQLLKEGPLIIKNIIQQIIMEKLEEYSLNKFGSNVVDCAIKCSDNQFKLKIMELLLSHNNQQVLFVRLSTNAYGNYVVQNFFKCADIEIQKELYLKITNNQQLLQEIQQYKFGQFVYQMLTQKLELENFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZA9</accession>
    <name>A0A8S1MZA9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0750023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CAMINEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1238"/>
            <end position="1256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1284" mass="153690" checksum="F6CE183A36C136B8" modified="2022-10-12" version="1">MVLSQQSQNIFIESDFDDQLQLKHIEQQIAKQIIAIPQIQNGVIISSLICEIFHLPLLGKYFCFLQRLNGYSIFCVDINQEILLIDKDSFQQKQLTYIEFKVETDFEENCEELFYDNSQFLVFCLISNQIRAKSINLQNITQIINVNQLNQIQQKNCKRKYIKISDDNYFLTHFQCYSWDILKFQNNKITLFLNSEIAQHNFNVNISYISNFQICSYYLLSNELFQLTYLLTDIGYHHLTIKSNNLLIYNYFIKKENLEKILFNSRCLPISFTYSSDSKVIFEFQNIKKEFDSNYFDSYYFNNILFLQTLKGLELSYNDILYQYLPINFIKLHIFPSFNLFYQIDNNTNQILLFQFLLHQNFIQPTKKYLYEIADYRLNYFISQCYYIENIQSNINGIYSLIFDNICQNVENLQLQKQATQLPKQYYIQIQDDPKTQILNITDQYQIHEKCAMINENNLESLKLNIIYHSQEFNVILMKGENILIFKHCDSGHLFKFTIFEQQVFFYKTKVILIRKNSKDIRLIQYQFDEIKQLNKQLKQDIQNILQFEQYILIMTQNKENYMLIDIQAQLIISQSQQMFDYLNQIKQTKIEENQEKQIFFYYLGDIICLQYNEQFISFFKQKYQYFVMKNLQIIKIQKVIINLHQILAVHQIQDILYLFYLSQEELIKIYEFSFDQAKIVRPLIYTINQNFLIIAGQQEQEYQLLIFTIFKNKNLQLIKTIQISKLQFFLENHYLYYFDLNFNIMVYNIKYFEFTLQNIDPIQINAISSQNLSFDIISLNSIETNKTIQIQLKQINLCSKLFAIDNQKTLLFKNNKSLKIQLDTIFLGCIDQLSLQNQNQIYLKGPILLKAKVNKNQIFSNFTIKTLYFYDSNINQQNKSNIIYIINIDTKDQIIVYNQEMKNFLNKEYGRLLDINYIDNQFLIIMFISHQKDLSYIRFLLKGNQFTLIDQQQLQSNIDVINEFEFYHTRNLIIFKQQLNYELWVVENQRIDKIKTTFQKSRVLFIQNTDKLYISVQFTQMNKEWEIQIFELEQLNIQLNIIRLIKRDIIISELNKYVVLQESNYISDQQIHLLEAQKLDNNVKLIMILNFNKFSILGRFNISLINLDYSFNVVKIIRTKIQEGILVPKYYDQNILIITQLQNYVYLFDLRQQKIMCDYINNLSIISFAIYAINSTHYLFHYVESDEFHIGEIDYELEIIDSSIEQVSVTLISQNSVSKAQVDLLILVEQDLQINRINITIIILILIILIVLLKLNCNRIKRKQTLNIQSNSSIELGQSNIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1C8</accession>
    <name>A0A8S1N1C8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0640213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1C8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="194" mass="22406" checksum="9D79D61D7364B67A" modified="2022-10-12" version="1">MSLFPTFKSVFKMLKTMNEGQPKLAQQVEQTQIVLSFSQQYKNNNINIENDGKIAICNSPELYKNHFVICEQKIPKNGCFSFSFKIGGWVMIGVCDKQKILEQKYIAQFEQSYGHGCYLIENDGTLYSSLKIEKSSEKAFSYTEYDIVSVLIDMDQKLIKWKKLNKTENFEMKIETSNDLYPCIGLGQAWVEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N255</accession>
    <name>A0A8S1N255_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0710242</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N255"/>
    <dbReference type="OMA" id="CASENQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035826136" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="222"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8084231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="40716" checksum="A25F013C256B861A" modified="2022-10-12" version="1">MRIALCLLVILAVANATSKFNLDYTKKRSISAVLAEVEAKLKNKSPLEAILNVLRDFRDAVNTEQVKHDEIYGIQQVECASENEFRSAEVQDASNVLRDSTAALNVAQTSKIRATSQSEVNQQQYFSAQEHMTSVQTSAESEAGYFKKRGRDYEDALHAIDEASDILASIYSGSGSFVEISRVAKNMLQTAFNIKETTRFAPIFYAFAQLATSEGQLDESALERVAQLLETLRANIQEAYNDYTESNAVSVAAFNDQKERLGQTIARLEAQHERLQNKLDSLSQQIGSSSAIAQRASGKLQRNQQLWDQAQALCATFDNEYNFATQARRNELQLVASLEELVESRFNQVNDENHERNQRIASHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N361</accession>
    <name>A0A8S1N361_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0760123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N361"/>
    <dbReference type="OMA" id="CCCHYYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="58830" checksum="BA61D950EED20C2C" modified="2022-10-12" version="1">MNQYQDMNEEQQAQGEEPQEQVFPQQQNEIQDIENTNEKFCCGLLNRSDHILIKVLKLIIAIPLLPLVIIWKILHCTTILLYNCCTNQLYHFLLYILGKIAQFLQQLWLLLLIFCHYMYSCFTFICNKLLDVIYCIGRCSKQILDWYAIFVVNVIFKFAHLKSIIQEFTSFITIPLTLKIILPLSMCFYHKIYTPTKTVIIYIIEKLFHIIKITTLTIFYFTVDYILKPIYKYIILNFLKFFVFLLVDVFYKIIIINFCKGMKWSIINFYTYILVNFYKYLIVKFLIEIVLTYSFKAIKLFLIDFLYKLIIIPICKIIKWMLIDFLYQIIIIKLYQCLRFIIVDVINKIIVKITEFIYFQICVNLYRCLSWIIINIIYEMILTNIFLYILKPLFNMLVSMIRIISNSIYQYLLLPLWNMCKYLFNGLIKIIGIIVSFIYFQLLTPIWNLIVSITKGLINLIKKLFSAARQLSIDIWESIRNLFRRLRNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEZ9</accession>
    <name>A0A8S1NEZ9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0800062</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEZ9"/>
    <dbReference type="OMA" id="YRIASVH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001577">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="5"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="426"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8088053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="456" mass="53374" checksum="8984FE1510C8C1EA" modified="2022-10-12" version="1">MLHQFQELEKLGSGSFSEVWKVTRRADHQVYAMKKIKMGTLNEKEKQNALNEIRLLASLNQEFIIGYKEAFYIDETQTLGIIMEYADGGDVAKQITNKKNKNQKFQEQDIWQALIQITQGLKELHEKLIFHRDVKSANIFIQNGIYKLGDLNVSKIAQRGLLYTQTGTPYYASPEIWRDEPYDNKSDIWSLGCVLYEMCNLHPPFQALDMEGLYKKIQKGMYPPINGYSNQLVQLIGQMLRLNASARPSCDQIINSNLMNSDFSRLYIKTPSIAINKRMMKTIQLPKNLSTLNDILPTKKYYNENKENDNESVRYSVQSSKLMPLNRKSVNNSCIEYEKRFYRIASVHEEILPTLPSEYRQPKVVTRRRPSSQYKEEEINLERRAEAIVSRKQLQKQLSEQHPLPKPILKKQMSNLPYLYRNVNDSERQENNKSKYHQSPNNYARRNIRKQITSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGR5</accession>
    <name>A0A8S1NGR5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0890037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGR5"/>
    <dbReference type="OMA" id="MDDSDAI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="48"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8091740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="349" mass="41718" checksum="D3B524F1CABB032A" modified="2022-10-12" version="1">MIRQFKCERLHYILNTNYIVAIYKDKLTIGKESHPKYEYKFKLENVLHWYVDNLKLQAFGIEYKGIIKFFKANYKDLEQLRSFCRNLICFDNIVELYKQIDINNPSRIQDQISLQLCGMKIVTQQQLLTQVPLIRQLNYNGIISYRECFQYKSQYYIITEFVGEITLQKFIIQNPKLSHLQCRAIILQLFKTVQYLHDNYVIHATIDINHIIYKSDFIKIIDFSQAKYTNILDDYDIKNCSRILFYMYTGKEYNDIDNEQNQQTLQNTQTPKLAQELILSIMTDKNLTVSQILEHPYFSFSLDPQERKKRFQKFQRIQRSTSEMDDSDAISQSIPELQTNKSKSLRQLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NIC9</accession>
    <name>A0A8S1NIC9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0840169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="105" mass="12339" checksum="F43EC50BD4A8C473" modified="2022-10-12" version="1" fragment="single">MRNSFNSGLDGNTMIIWSPLNSFEFTIPTNQKDQCILKEHTNFILCLHILTPSETSFLVDLMIGRQNFGLFHQQSFLYHGNVHRLLKNRELKFLDYQLIKKVIDX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJ55</accession>
    <name>A0A8S1NJ55_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0900153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJ55"/>
    <dbReference type="OMA" id="IESPNMT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF155">
        <property type="entry name" value="DEUBIQUITINASE MYSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="98"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8092370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="177" mass="21120" checksum="D8915F914969A9CE" modified="2022-10-12" version="1">MNPDLMCNLYSQAQYYQPYIYFPHQQFFQYMILQKQTSTMPIPSQKYDTPIFLNNGITQQSIESPNMTKTLQNNHLTEVDFEHQEKCSSIQDESQSRVNGHWTKQEHLLYLEFVKNHESILRSKYDKKSKKIFKLMSQYIPTRTATQCRSHHQKFNPLTKSKKKCRQTNKIPTVIHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NN02</accession>
    <name>A0A8S1NN02_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0880167</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NN02"/>
    <dbReference type="OMA" id="PYFNCKL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000683">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="103"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8091631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="490" mass="57657" checksum="2B5064BC96FD4F33" modified="2022-10-12" version="1">MQAIYQMSCIRKHILKSKTYYLEVHDNQMILSEHKNMQNAKYVVQYIWETRVMWKTSENEELCAFGIYYNNRIKWFDSDHNSLNLLKIYLSARVFFGNISIFYDSNQILGQGASSKVCLVKCKKSISHYAAKCISKKYLLQNKSDDRINRLRQEIQILQRIDHPSFVKLYEIYQGENSYYIVTDYLEGHTLYNYIKNYPEDQLPQYQIREAIKIILTALNYLDSINIIHRDIKLENILLQKPNDITSLKIIDFGLAIYQQPLQKLCVCGTPGYIAPEILKSNHDTDYFTQKCDIFSAGVIFYKLLTKKTLFRAENTLEIMEKNKQCQINFQEFEFKLQKESLSLLKAMLNPNPKTRYSAQQCLDHPYFNCKLENINILQEILDKATGFSGITQINYQKDTLSIGQECQAAQIKRCNPTSYKIRIEQRKQTKSPRKYADFQFYYPSFCQMNSFESLSSKGSSYNISNIDNLINDKLDSVIEEEEKQQIYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPQ2</accession>
    <name>A0A8S1NPQ2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0880143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPQ2"/>
    <dbReference type="OMA" id="MNTDMMH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="340"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="583" mass="68117" checksum="3A31088C796F9DCA" modified="2022-10-12" version="1">MSESPHQLKAKIDFLHQENQQLRNNIEDLQTVIKLNKQSMLSMIDQVVTIPRHNQQTSIHENENTVKALKLVITEQQKEVVHLMSSLDKVQKERDFAQGKALISEEITYAAEKSEKKLIAELESTIYELNHTISQQAQKIGQLEKQVDSLDDTTGVVVRYRDVIHPNSATILLHEEMETLHQLIVRLQLQVNYLKSIQLKLVTLINKVITNLQMKNQGELIDLDEDYEKAQHEFLEILAQIQQSRLPTVELPVRINSIQSPMNTDMMHSPILSPFPNKAPQVQKKNYQTLNQTFDGAIGINDSYQQNGRQEDLQKNISRVPKLNLARAFQIQQINAKRSTQQIKLTEDQLQNRVRKLELELEETKKNLSREMILCKHYEIENDQLKRYIKQIEANNDILVKSNLNLNQKWEKIQKSFMYYKTFFVEHKDQYAQLQILVQSEKEKAQSQEDQNEFGFNSVRVSHFRKGQTQRKKTLLDQTITSYLPLYQESLMEIENQLGRVNQSVTYNQNHRINSSCAIENNGESPTIYFDKNDSDNENTEWRSYIKREWTILATQVHSAKPKNLVQQIKQSQPIQKLQNIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NSP4</accession>
    <name>A0A8S1NSP4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1010003</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1830017</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000192">
        <property type="protein sequence ID" value="CAD8116911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NSP4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="205" mass="25266" checksum="4C63D01A661E1334" modified="2022-10-12" version="1">MPAMRNCCYLKLNSTALFNENLTQENSFLGNWKKQFLTTSSKLIIIYIFEYNKLQEMIKKFDEFTNIKFTCTHNNNKLKQKFMELQNRDVFYLGYQEKFRIKKNRQSHTLLIISKRLIIIIIKQRTHKRMKNFQKYKLLQNLDLGQPNGQFKRFQDLTKERTIYNKNFQVFQDLFKNQRNLIYQYNPQNLKQQEQNQWRLYFHCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NW23</accession>
    <name>A0A8S1NW23_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0940008</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NW23"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="223"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="257"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="291"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="128"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8093693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="44454" checksum="5DC297B2224B1955" modified="2022-10-12" version="1">MQKCTYVNHENEVIMGICLNSNCQHASKYCYKCLFEVHQEHQKHCIRLIDLPDLIKIYISDQAELIKTSNVIYNRVKQKMDQNIKRMDENNQTLQKLNQALQNQDFIISKQSIDLIKKYYSQDQQNQEKQFINQLKEIEKILESKENKKSELQEVEILFNMAMQLTNQGQHEEALKIYDNSLSLLNIIDKIFMWKGQSLLHLSRLQEGIKCCDLAIQLNPENDMAYNNKAWALENLYRFKEAIECCDKAIQLNPKNDMAFNNKGWALSNLKKYQEASECYDKAIQLNSLNANAYNNKAIVLHQMKEFQQAIKFYDKALLIQENSTFYKYKGIHFLKLTILAESLLALGNKQEARICFMKALNLGYVDQDYIRRQLLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWT7</accession>
    <name>A0A8S1NWT7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0970024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWT7"/>
    <dbReference type="OMA" id="ETAPAMC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="146" mass="17065" checksum="E76FA2853B554EB5" modified="2022-10-12" version="1">MDKELIIDCQQNKETIGLQPNQTRKVQHDIFLKKSSSFLQNQQSKYGIRQPIQLKNPLLIKSLFQPKKFFKKRDSSIPICTLKTLPTQPLFSYQTDRIHSRCPAFKNIRLKSGFKQSRHKTEEKIRIETAPAMCRSPVPIQDLNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZ96</accession>
    <name>A0A8S1NZ96_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="6">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PPRIM_AZ9-3.1.T0910151</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peptidase C19 family. UBP8 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase C19 family. USP20/USP33 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZ96"/>
    <dbReference type="OMA" id="CENIDCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF33">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="22">
        <location>
            <begin position="17"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="21">
        <location>
            <begin position="148"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="DUSP" evidence="23">
        <location>
            <begin position="639"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00038490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CAD8092834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="792" mass="93034" checksum="B73717027B88BA8D" modified="2022-10-12" version="1">MSSIKEKNICIHYHKVLLQRSLPKFFQEIQKLQVLYQKNNLKCEQCQYKMDIGNICLRCQAVICDIEKQCLHHHNQQKSDHCIFINLLSTQMTCIICKLEILDFEQYGLTGNEDVFSQWINPQRIRIEYLRYFQKQELSIQRFNSPNVGILNIANFSFMNSVLQILLNFPTIQQILSKVHIEMEKGDFNIKSIKKIILRNICCMSDALKSKQVKLLNPTELIKSIAQSESKLLGYGQKDAYEAFKYIINFLHEEFRFTNSVSYWKNIIPFQNQEAITHDWLLLSQPSKFRDYSFIQNVFSGELQSTIQCLNCNRLTLVNENYSAISLEIPQNNEQNVISKFFNKLTKDRQNSVTLQNCLDLFYEPIQETNFLCLCGSRNGRRTYKFSKFSNILAIHIQRFKNKHQKDQTYVSFPVEQTNFSNLLNMQGDLNYQLFGLIVHYSNVGGDHYESIIRQPNNTFISIRDDQINPVTLKYVKESEAIMLFYQKVDSPTNYKTIHNVLTQYELIQTGEIVLKDDELSFLPNFWFEQFLTLSNPQMIITSHLLCFHNQLKPDYWDFRQSYENVNSQEQMNASFLCDQSINLQQNNNNNNSSSYFQLQLQSVQLPTAICQFLVEKYGGGPLIQKNIQTCTICIEQAQNMSKRRKLERSLIQKYEDCQSDRQFIINLDWIIMWQRYLYNSKQQLQKNFIFGNPPPGEINNLHLLDSNHQLLQDKQVDKDYSLISHQLWTVLVSIYGGGPIIQVSSSSNEQDNPKLKTFNLDPEDNIKIKEIRTILNQCFKMYNICENIDCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P329</accession>
    <name>A0A8S1P329_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1040022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P329"/>
    <dbReference type="OMA" id="TWVAGWH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="173" mass="20462" checksum="0EE7F8166B7C7A79" modified="2022-10-12" version="1">MIKKFSYFTYESLPVYTMVTSLVLDNSVLQFYLVNPTFAITDQNSMMSFSKYSKTKFALQLRLSTEIEQIDDSLKIKRWGSFGMNYEELSQLLYPNLLPGQQFALQNQQKQLLGKLYDSHFKLIFKENPDQENQLTYIINPEQHGQLRKQIEKSLTWIAGWHHPKQFLKHLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P430</accession>
    <name>A0A8S1P430_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1050026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P430"/>
    <dbReference type="OMA" id="WSVNEEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="678"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8097768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="794" mass="94589" checksum="BF7E1BC040669241" modified="2022-10-12" version="1">MGCMNASGYISGPIEPFYQSNKSNFEAFLIEQLTLLGFQESQIDISKTIEIKFIPYLKELQTSIQQDIHSPFNNNIYLVPVLTNQLREIYIKTSEFLIRFQDYKLNTLINKVGLKYQALLRIGDLFPKQLNLRFYNNQISKIQIDPNIIIIVINHIDIGYTLLQFIRELNQNFLLVFRQDDNDNQNINLFIETNSFQSCSVIIGVDDKEDRYNTIINLYTKVIKKQKKNQNSDSHSRILTQFLNQEDQEQFWEDGTLIFNFLNSTQTHISNNTSIITIQNHKIISINEFNIKDLQFQSLKSLQDKKPIYQYIYKSIKQIMIKELIQALQLKSSQFSQYFKLKLSTQKIYKDLKLQQKIYMPSQLKLQNVNEIDNKTQEILTIIKKASHDTLIIKQSSKFQLPSKVRIYQILQLIFKDQDVSQVQIIINSILHIQWSLGDEIINSQFNIQNQNYQNYKYKWKMIKFYDNYKITNLAQPKKQQIFFQAQQLLKCLNRKSDLEILNKIINQIMIDKWSVNEEYFPICDVYQISFINSTSVDETSQYQEKLDKIVVSQENQYLIIFLMDYQDPQRMSQLDQIISLPLQMDIKLIILSNSEFDNDNFEQLINKKKIEKRIYQKNIQFWFPISSQINEMRFSQYLEIFYNLNQKENVIIINNKNQLCHKITGFQLIKMLQERNYQEDDKKQNQNNNTSAGKRQHQNNNNEQLKKARQQLKQHIEKEIKQQQQLSLQNEQDHSTNMILDFQWAKEKSIKMDGQQKISVISKQYFKPIFNNNSGIQFEIFQQIISNKQYDFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P510</accession>
    <name>A0A8S1P510_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1050124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P510"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="46502" checksum="D4AC1C53C93CC00A" modified="2022-10-12" version="1">MYTKFNHRPVQEFAYQRKPKIKVLTKLGFQELMKLQSNPVQFEEQEDLTLHPFTKKYRSQINLWEKLKQVENEVIGDEETLESNEFEMLNKEHPYILRNHAYPLQDQYEEPIKLPQIQTERYQKSNAPKIWESSLFTGERVQLRRRKLKRQEAFKLADSLSIINLNLKMSDTEIESLNPYYKDIVAYLHTQQILIYQQHDLLDEFKILSQFQYRLKLKISTLYLAKSIYLRTNILTQDYLLTSLWIATKFDQQYRCKWQMSTEIETHILQKLNFEITKPTVMDFIEQQIYYIKKTKRISAEQVRLIYALSLMALPWTLNYEPSQIAFAIIIISFTLKNIKNHDFQSDCYEDFRQQLLIKYDQNIIISQKYPSSYLLIKKNLHRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P515</accession>
    <name>A0A8S1P515_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1060044</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P515"/>
    <dbReference type="OMA" id="YCYINNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327:SF51">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR OF YEAST FORM ADHERENCE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="53"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="129"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8098089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="415" mass="48875" checksum="DF14AC9C63305DFA" modified="2022-10-12" version="1">MLSKIQLIIKIISEKQEHFKNLLFQIILIQLFSKYQSLILVYKDNMQKIQFNCTICLNHLSDPTCLSCGHTFCEKCINHHLQLNHSCPLCRKPTLSEWPVNEMLKEVLLILFEQGSEVLQTEFPQIVLCLDCGLTPINPVVLPCQHLFCLQCYQNDLIEQYCPVCLQQCMITEPNINLLFNQFLQWYCETDEKFILQNQQIRSMPIFLFDKQICYKRNFSLRIVEDRYKQLIKLASHGSGKFPVVPFTDQLPVYADMVELVSITDQIEIIGCDRLIIDAIYCYINNEKIPFTPNTFKALPNQLWLCNYTIIKDNQDKSTLWNKIKQFIQITFTELTEEMKSLFEKPYHLMSIFGNKFHQVNSSLIMIQLLKINNQQFQKLYYSNDEQEINSFGILLIKFNKKSFKLLVIKIKVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8G6</accession>
    <name>A0A8S1P8G6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1090132</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8G6"/>
    <dbReference type="OMA" id="INYGDYV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.70:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3260:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="entry name" value="Ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_eL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_eL21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20981">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8099406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="18299" checksum="A435FEF093040DDB" modified="2022-10-12" version="1">MTHSYGLKKGTRSKFAKPFRGHGNISIRKTLQTFKRGDFVDILVDGAQHKGVPFQYYHGRTARVFNVNPRGIGVSLQRRVRGRYVEKRFHVRADHLRPSKCREEFVKRVQENDKKKTEANKKKQHISTKRQPVLPRGAEIVKPAATVFQHPKAFVEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PB62</accession>
    <name>A0A8S1PB62_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Casein kinase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1120020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PB62"/>
    <dbReference type="OMA" id="WHGEYKK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001186">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="14"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8100265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="454" mass="52048" checksum="191FCCC596C70E9E" modified="2022-10-12" version="1">MLQIQIGDIIDNQYEIIKRLSQGSFGIVFYGKSIKTNKGVAIKVEKNEMASFGTLVREIEMLSKLKGVPSIPELIWHGEYKKCNIMITKMLGNDLIYYQKQYIQFSSQCINSIAYQLLWILEQMHKKNIIHRDIKPENILSKNGSDKIYLIDFGISKETDSNLIHKKKQFSFIGTSRYASIAAHQGIEQTAKDDLESLGYVLIYLTTQRLPWMNIEKTDVQRLEKIGKLKENVIMDDLCCGCPNSILKFMKYIKELKPNSKPKYALLRGLFLSKLQNTVELGLDWTNQVIKLENKKNHKKQRNSCNVNFQSCVTIRKPFVGGTQTNLNVKTVAVQDEQMISSDNGTSQMQFSIVETQGSKNSVKVGFSTSDNLDCLAIQQNNNMARVSTFEGIAEANPLLPQNLEQQLMEKENQDLEIKYNLLHFTSVYFNFKNPIQQHTIKQNYQVNVIKSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCP5</accession>
    <name>A0A8S1PCP5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1140032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8100946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCP5"/>
    <dbReference type="OMA" id="EDPIQED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="73"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="158" mass="19146" checksum="A45FDF0D7C0D9D5B" modified="2022-10-12" version="1">MHQELQKLEQLNSQELELVKQVYEQKVVYLNTKIYMLSKELEQKTSEVSKIKRSQIVVQEDPIQEDNLIEIERAKTQDLIKETKEQMEQELLELKSQYSKELFELQKQLRKRDLMIEQLMIEIQMYKNCQNQNLDKMKVEYLSQLIEIVQDIQIQIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PE87</accession>
    <name>A0A8S1PE87_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1150036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PE87"/>
    <dbReference type="OMA" id="CVCKSSK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="130" mass="14924" checksum="C5A7C8EDC8E48652" modified="2022-10-12" version="1">MSVSSVPKGGLNQGVIVPLIFQCPGDCECKSSQPKTWYHAKCGDPSFITEFGDILCKNHLKECSGYFFQDAFFQCQMSNKSNTWYQYKSQTFLLLALSFVINAAEFILENNDLNNFINQILKQVHKRWHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PEF0</accession>
    <name>A0A8S1PEF0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1150001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035717946" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8101221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="21378" checksum="1905232B988DC389" modified="2022-10-12" version="1">MILIFLLCLDALKSIEIQTLDPYYQVAYQSVKIDDEIIQPNQFFSYGLWSRYNPLSAISQVGPVGIFGSNCYQLLNIIDKVDGGITFMYFDCLHNEKYQITKEIKFVNDNGEQKVYETQXLSNKMHNFIITLSISTGEHYFLNLLKHQPYFQHFFIQNNKENHFLNLLALILIILIGIIWITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGE8</accession>
    <name>A0A8S1PGE8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1170161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FDINFIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1106"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1308"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1412"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1625"/>
            <end position="1647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1683" mass="200027" checksum="66977427EF1E05FB" modified="2022-10-12" version="1">MFLASEKKGLTFIEQYITMMIILKKQHQLTRLASAIIIVIPYIQLLILTLDLRLSENTKKESIHFQIVKSFIRPDIWLIEKENEQQSLLFIIVLVIMLFQKAKLIIIVILYYYILNQDNLLAHLQNNFYIKIPILILSLYQQIISLILYQWCVLICVCSIKNLFDQIVINQQFLNLFLIFLVLLTLILVVLDNILHIKVIEKPITMKIQGLERKNTSTLRYVSLIIEFIVQFLYIFQKDQPSTLHLLIGLSLLKCCLDLYDQFTNYVFVNGKLIKVLICGISFSIIVNFLIEIIELQDKYGSRVIFQTLVLTPILFQILLKYYQIDQKQNLVTLFNDQIVSPKRFIYHITSILNEQNFEFCTIQRSLIQQYHYKKCQNLSCSICGHQYILDTRSAQGITIEIFKEFIQSRLIILLKESLKLNFTDQQSCKKSKRRFLSYVFLLYDFGWIMQSLQYLHQLKNAQQTQQSNLKGLKNHLGTLPQSNSYSDRNSHYVTGDNQTNLSQPQNIDANMQYQINILLNQIKFSMLESIFISVTLEEARRTIQKNFGTDDYVQQQSITSDFVTQYLLNEVQVQQSINIIINLVKQKIKFYEDMMKIKSDDKKVNFWDNHQSYQNLIDKCKQFNDGESKIIEMYNIHPSYILHQALNVFYCFIQNDYEKAMQFSRSQKFLDERQLKFLKIKNFNVNSKDIYYLIMTLQDDMQHFNILTHSNQLFDLFGVFRNKGENYQFEDLIPLAVVNYHHHFVGQFLETGQCKFFKIFDINFIVTKDQFIQAIYLNVDVTELFVKSNLTFSAFFQPPTEDLMFILVDGVKHQCLFTKSILNQIGWNSEQIQWFKVNYDINKISIESIMPDFLDILESLIKLTETSLSQIELIFPKPPEKLSQKQYDFQSLKNIRNSYFRSNTIQNIKYICDLTIRKRQISNFVYYVMHISYIQKQQNVESNLENSTNRQRSNIINDCDFDSEINDLELKNRNQIFQEFQKQVNNSTKQMQRNVKLFSNYTNDQIQEEILSPGRICITNHNNQDKNDINEFKELLHSNKDNQQQYFCHDDQIKDCSSYELIISQFTRKQVKQQKTKTLNDISKDSLWKKYDIIHRIMQNSKPKYIVHFLIYTFLLLISFLIIPIFIMYEFNKDLNILIEQIEMLQLHASIMGPHDLFFSMRITVTSYQIQYREGFINAKELQVLTTPFLQYLPLGYQELQDNFYTQLTNPYLAQFLNDVTIPVWFMGENSSVIYSQDVTFRDNLFIMLSYQQQQLSAYMFNRAIAGQPFQVFLIANYLIMQNTCESLTREIKVYSEEKCNQIYIKWSILLTCFVFVFIFLLLIVFYYQRKYFASYDLIIGLLRHNTDEIILNEISRQKTLLNIISATMLDEFHLEKEYFMIIKIQNSTENAKQNQLIYHKFAQQYPQYKYNIANIIFLLLIIIYGIIVFVQAQQYLTKYNVTTDFFKLIQDLKFRGGNGFLYRELFFRFSSYPFLTKSDQENLYILLNRSQNVLQEFNSLIETIHYDDYLLTQNFIDLLELVQVNEMCSQVDENFKPLMDQYCQLSFEGILSKGIVPTLSYLYNQIQTQQQMNNFTKRVEMHKYELEGAQIITRAFFALSNSFRSSFKEITEETLKQLNNVSISYLVFCGITILALILLYPKYLFNQYQLIKRIVYFIPLQIFLRDGYLERNLKEILIKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKY6</accession>
    <name>A0A8S1PKY6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1210196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="195"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="237"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="279"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="321"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="363"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="404"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="446"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="470" mass="51739" checksum="C01323DCE6C0452A" modified="2022-10-12" version="1" fragment="single">MFGFIFEKLQKEKVEDCIGFLSDIENQKQLESYILQKGENITETVKEQKLSVLRKDMKLITDVLKKIQDHDFNFKDFSSEKNGESTLSLINSIKDNIRIIEFLQLLVHLTSIDDKFIQGGSNSLHLLVQIKVDLRNKNLENIKIKNTSLVGANFVRCNFNGSQFNNVNISGINLNGAQLFNCRWKNIKVHELNKLDGRSNTVQSVCFSPDGNTLASGSKDNSIRLWDVKTGHQKAKLDGHSQSVISVCFSPDGNILASGSRDNSILIWNNQTKKKVAKLDGHSSGVLSICFCPDGNTLASGSKDNSICLWDVKTGQQKAKLDGHSDSVISVCFSPDGNILASGSRDNSILIWNIKTKKQIAKLDGHSDKVQSVCFSPGGNILASGSYQEIRLWDVKIGQQKAKLDGHSDQIWSVCFSPDGNTLASGSDDKSIRLWDVKTGQQQAKLDGHSACVRSVCFSPDGNTLASGSX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMJ8</accession>
    <name>A0A8S1PMJ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1240014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMJ8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="246" mass="29415" checksum="CCE164FF856F7241" modified="2022-10-12" version="1">MQEKISITQKIIYDLEVLLGNVQIEISNSINLVENVLKKINLYLLSYMKQVQFNNKQSSLQDFMSQNRVVIQREDLMLQTNSKQYEERVLTDHQTALNILERALAIIQKFISREQDINLRDPAQRDSQELSQCLNKRQPLQQRATFENMKQIRQKILKVIQESINILKTYFMILAQLEKVHLIFEQQIDDNLVSRNTNLQKIKVDNDFLDMKREDVKMYTQKRQDQNETFAYQFAKFQDLKKYVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQA7</accession>
    <name>A0A8S1PQA7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCR4-NOT transcription complex subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1260032</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNOT11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQA7"/>
    <dbReference type="OMA" id="MYLTKCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019312">
        <property type="entry name" value="CNOT11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15975">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15975:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10155">
        <property type="entry name" value="CNOT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8105064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="346" mass="40171" checksum="B96D81CE897BAC79" modified="2022-10-12" version="1">MISKNQIDQVYDFLKSINDGTLESYRKSLPEQWILNQVIDLTCLTPSQRIVCLYYAGEETQCGNALEKRLVLNKEQCGQCNLRQLVDEWSTSNQQNTHQPQSMRAIIQCPSLKHALEHILTTFSIDYLRPVPTTIQSQFNWITLQITDPLWDYTIEVSQEPLVRIKENLLKAQQSKLSQREAQETIDLLNQDQRHVLDSGITPNQLPNLIEYNPDLSSFLLARINQCGISIHEYFECLIQMKISLQSLEVVNKLSNSIKLPEAFLHMYLTKCIQYCEELQPKQQMVSRYVRLVAVFIKTLIKSKTLDPKKMFTEIQGFCIEFSKIPEASQLFKQLKETGVEEKQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRV5</accession>
    <name>A0A8S1PRV5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1270032</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRV5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06627">
        <property type="entry name" value="STKc_Cdc7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF4">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="58"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8105359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="848" mass="97531" checksum="6DCA1CC07638146D" modified="2022-10-12" version="1">MALFNIRLLTCILYLHASLQSLLYIYIYQIILNVSIVQIVEEDRQLLARRFARKRFDWYCVQRIEFGISNKHTIIEGTLVAIKQVSRATLKEDQYKALQQEIYLLKKLKHENIVKYIDCIETDQFLNIILEYIESGSLASILKKFGSFPESLVAIYVKQVLKGLEYLHQQGIVHRDIKGANILTTKDGTVKLADFGVATTLSEDTTQSNNIVGTPYWMAPEVIEMSGNLSTSCDIWSLGCTVIELLTGNPPYFDRLQYAAMFQIVQRDCPPLPEGISNECRDFLIQCFQKDPTLRDDATTMLKHIWITKSWHIVQGPNLPEEVTNTIRNHIVDQSNFGTFITDSFIEQVPQLVQCQSPRKIDKSSAVRDSQLDSLRQLKLRTSHQIKQNGFWGQNEDGKSMREINELITQLTEQNDNSQIIKILELLKDTIPNDKCKEYFIHTGLTSLLEIIEKYMPLYCENNSQDQQSLQILKLIFELINIVVDQNPQLLELSCYLGAATFYQQITSFEFHKELRVEAAYFLGQLTYFKEGYLLSIGGLDNLLELIDTSDISNNIDLIGLALDTIILLYDLNTISQRNLSRLLISKFITYRLVLIAEQIQNIDVNLMIKSLNILVILAKCEDKIVKQSLCEKEVITRLATFLNQNQEQALLKTIKIFRYLSTEPNLQPHLDGFAIIPRALTLLKKDLPSQGPMIADILRIIFYLTKLNHDKQEELCLYEGVPLLLELFKLPSEQENQISMKKAAISILCSLATSSERCKQKLQENGGITIYVGLLTTNLNITKLLDTIVKLIELDQTQRIPLSKNIDILVGYFKNNTVPMYYLKNLDLVYYFENNFWNSQYKRIRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRX9</accession>
    <name>A0A8S1PRX9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1270054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sel-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRX9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050767">
        <property type="entry name" value="Sel1_AlgK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102">
        <property type="entry name" value="SEL-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102:SF147">
        <property type="entry name" value="SEL1L ADAPTOR SUBUNIT OF ERAD E3 UBIQUITIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="644"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="645"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8105403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="691" mass="80818" checksum="EF10376A76DB2787" modified="2022-10-12" version="1">MNFLLPQQPIESDQQFYDGFKYGIAHQQISDMMSQVQMSSNIDQPRKTFSHLVFTKMSHILKNYSTQQYKVSIKINQELHYLVRDTIRTISEAFEACQQVDKVQLCGKDASFKLYDKYMRQLDQLSFFWEQLHSLENRSINLNKENISSEISQYLDQWLDTLLQKLDFKQTSQRLQQIQQSKPIQATKIQTPVDDRLRNAHELFAGLGREKDQIAALQLYTKLAQENNAIAQAIMGQICLEGITGPKDYDQAFNYFKKSADQRNTFSLYNTSKLVLEGKVYDKFYDKDDNNTIVSKYSSEVYNRNYDCNFALTLLKRAAELDHLQSMIYLGDIYSNGLQLQDYTLEKDYSNAEFYYKQAKNKNSTQAMHKLALLYQTMCKQPLYKNRSQLIQPLLNQAKNLDYLPAFYDLGLLLYQGLQDELEQNQVMADLIFEQGCMKGDLKCAQKLLSIRFQALRKNEAVVEDFFSLLDQIEENNKDWTITYYMRGKVYEKGVQVEKNQKKAQEQYRLGYLKGCQKCRVQLDKYQRETVPTDNNPTNSQQSSLNQKQTINGFIEQVRQSEWRRPFQKQRQVSIGNVQQIYNNVDNDIQSPIQDRQTRMTISITRPKNQTDDRKRGVSAIQLPHQEIGRSILLSQMKSPEKVNQHSQFWSPRLPSQQSQAGSQITNSTTKKRFNLNKIRLQINVPENLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTU8</accession>
    <name>A0A8S1PTU8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1310038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTU8"/>
    <dbReference type="OMA" id="KVYQFYV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8106536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="28664" checksum="EDC1D78F95C59257" modified="2022-10-12" version="1">MEDQEKEKSRKPMTLSQRIRMELSQQQEERKPVEGPQLIKHKPSSYDEETGQLYSFYASAVNQTQQFFSNIFGGTYIPQYQFSFKHKQNVIKYVFEKNNVIAEYVSKEMRKSQKDMDLNELLQLQNRMMLVAQLADCRNRIGINAYCWGALMGSIFMLLPISIWKKGAMSMSVFHLFGSYVSQANIDRVFDKVYQFYVYDLEQYKKERENMTVGQIKQSKLDRDLEDKRLRKYLREISKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PW16</accession>
    <name>A0A8S1PW16_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyltransferase 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1330080</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PW16"/>
    <dbReference type="OMA" id="PELMYPE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF0">
        <property type="entry name" value="O-ACYLTRANSFERASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035817053" description="Acyltransferase 3 domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="369"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="510"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="549"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="591"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="631"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="661"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="Acyltransferase 3" evidence="3">
        <location>
            <begin position="282"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8107262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="716" mass="82967" checksum="5DE23C9112972045" modified="2022-10-12" version="1">MKFLHYSLCFLLLIMQIQSNDENNVKCEEYFKNIVDAISIGEMPKNVSPQTLLSSGKGLQDLGSYLVCRDSVDLHYIHLRMISGGRPLHMGFCLPQFCDMDYLQTFNETTKKFINNLTNLQFNRSMIESVSYQDPELMYPEWSVGTYLTLFLLIVVVGLNVIGSIFSIIAENKKKAYQDDLQIEIKNKAAQKINDSKIEETSFQSESINQLYRRSELIQNEIMENQESAFMSGQGQFEEIKYEKYKEATWERFALSFSFIHNYQKLFKINEPQGDNAGFVVFNGIRSLSIFWVIFGHDQLIRGSNSFNLIDIPYKITEPGWVTLTPAAYFAVDTFFFVGGFLAAVLLLEKLTKLRTIKFTLVPAMWLHRFLRIWPTYAFCILVYWQLTVYMSDGPIWNEYIASTSTCNTQWWKNLLFIDNMFSHANNGLSYCFGWGWYLSNDFQIFLITPILLIIYARNKKIGLTIIISLLIGSVFSAYYIAFSHDYHLQIGTPNAKPQPDYQDVFYYKPWVRISPYLIGILFGLFYRNYISGQNQKLVDLAKKIKDSVLIRTLLYLIGIGLTQTIIWIIIPLQKDMNAWPPQAQYFYQAFNRVFFVIGVGFCITPALLGCKNDPSRFILGHPFWQPIARISFCMYLTHFIVILFMTFSSTQLVYYQYSHILFFTLTDIVYTIVIGGILSLAIEVPCMNLEKILFAPKKQQAIKLNGNQVQDQIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZA9</accession>
    <name>A0A8S1PZA9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1370125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZA9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="465"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="195"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8108570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="59891" checksum="91257416D7D95DCC" modified="2022-10-12" version="1">MNIKVKFPNGHFLEFQCPNHWTTEQICQFSARNQSFYDPETIKLYHGGLELNGNEKLHEIVKKQEQIVFLTVIGNKKKPSQTNKQLLYLEYTKNIQRVQFANQFTLSNLQPIISNMPIMGLQYQQNASEKIQKQQQQQQQRILDDRDSPMQQEIFFHFKMICYIGAFYTLFSSYFKGSNYYYLLVIIGIYYWIKYQEFKAQLRQQLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQENIKNKNKEKIIQPQNLSNNSKLNQNNFEQQIEQQENLDELDSFNDYENEQDFQIDSKEHLQDFLIRQAKILQAQELQQQDQMGKIVSKQESNQQINDNEKKIQRQQENKFKVSSDQKTQLIDTQEQIFKEEEDLQFNNKQSQENVQEIISKEAAQINSQNKIDEPQMQNNEKKVGENLKENEIPDENIQEIQYEKQEGEDQDEEPELEDEVRRLKQQQQNQKITISTFFKAVFEFIYCYIMSLFPFWHVGLYQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2T2</accession>
    <name>A0A8S1Q2T2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1420108</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000146">
        <property type="protein sequence ID" value="CAD8109996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2T2"/>
    <dbReference type="OMA" id="RWIALIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="601"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="708"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="607"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8109996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="753" mass="90054" checksum="D30929C1FE43ACD4" modified="2022-10-12" version="1">MYQLMLQKQKQEEFNTKFITRAKKGMLKRFFFYDRTLNYIETLIFTEFSGRKQIIPMRNTQCLKPTCIYDIDELYERYEKSNYSSYANFMCHTCRKMIDLQGFYMDEDLRKIIDVIWNTYNKFSIQCQKAAIFKDGSWEAVLPEYLKRMYRKNVEEYDVKQNDDDFIDQPDEIIKFELPLLHEFDVQEYAELLEQEDRGDERHHDSDILLRRYNLQIKWGEFAQFKNQVAVSINIMGIFMHYLHDYLEDRKNTMKMQAKLKLGVLCIKVISYDRFSKRAEFFFLSDNLEGPKANYTEYEQVIIAIYYDERWIALICDIEARRLKMIDFLEETIARTTLDEVYELGQYLISQIFQPYKFPKNPSRELLIGQIQEDMRNEIPRKIQLYCDCGHHVAQYFYYYILEEIELIKIQQKWPQDVGVKQKIHNVKYKLGEQDYFTAKICWVLLKMSQNTGFKILVYDMKPKERVEKDTLDDFVIEQARLKAEKLWDDNNKQPKQTTFQIVHGLNQVEDVVISKSGIDKQEDQKQNKQKSRVEKYWGVESGIQFNNQRNLQPNYAQQQYQQKDNSLLSNLSFQFGNQDKPTVRHQSQIQDDSKSIQDVLGQLSGNNNNNNKNNNKNNNKNNNNNNNNNNNNNNQKKEKPFLVMPKAQLQQYLSDYKQQIIKEYKQQALLKGYKQQLIGEFNVSQKKKPGEYDSKQLTESQYHRLLLNLDKSLNQSEKKELKQLQSELGLNEQDLANLDAYQYDQLLDMMNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q3J8</accession>
    <name>A0A8S1Q3J8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1430071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000147">
        <property type="protein sequence ID" value="CAD8110209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q3J8"/>
    <dbReference type="OMA" id="KQANDVN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="167" mass="20059" checksum="E575F4511CF7D9C0" modified="2022-10-12" version="1">MGKKKPDSFLSQLSKLFNGEKIIKLTIKRNLKEQYPQLRKRLRRKQKQQLLSEIKQVNDINEIIIRSQTQKLTISTSSNKQQINKFADSLSNIYRLYCHDQQKQIKKKHVLLPTQPNAKSKTQQRLKAKKLRNKERRQKRHELSKLKKKDLQRQKTFASKKREDQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q660</accession>
    <name>A0A8S1Q660_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1460072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8111052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q660"/>
    <dbReference type="OMA" id="ENDCSHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="102"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="359"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="505"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8111052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1047" mass="126229" checksum="DA21FC4F1893F270" modified="2022-10-12" version="1">MHRSSHKRQRTSNTPKRQKEQDNNPIPSQQVAKQVRNDEAVQQLSVIDNPKNQLRRQDSQNFHLRSSQNLEKSYNLTDRIISPARKLDQSPNLRKKENIKIIDSYEARFGKLSTENLQLQNQIQADQQKYNKIINELEIQLSNQTYKLQTEQHNIKQQQLLLQKKNEEIQLLVNELKKNETKFIQLSQNNAQNVDENQNLKSHIQNLEYELQNLKQQVQDSHFKSARQIEETKIKVENDCSHQSQIQLKDIQQQHQQILSKKNDQLIQEQQKYSKLLGQFDNLVNEKNKMQLLIDDLQDNKLAIQQQIHQSNQMCEELRLKNISLTKDLQLIHDKQTQQELLHQKQIQELKQYYEQQQINMLQRKLNETELKHSTENQQLDQIIRDLQSQLQESENNYQVMKTENAKLYSIDQEQEKEIKILQQELERINKFKDLEIQQLQEDLQKLKQQLLEQSSKYQTKLHELSKQQVIANDIKSELELQKNVTKEIEANIREQWETDILELKSFHQQQIQHLEEQIKYLNIENDKLTIQNRKKDEQLNEYKLNQQLLDNSINSLKHQLSETEKQKFKELEDYQFELENFKKELKEQNIKLQQEKQIMEQSLKQQKQKVKELETLKIELEFSSSQQIQNYQEQIKQKDWQINQFNQEKQKFDSLFKQTKEKLIQAEFQVVELKQSADIYLKELEESTQQRDLERNQFNQKFSFQETRISQYKQAINQYEIEIHNQNNQIDKSQKQLDHAMLESQKKIEESRKKEQKLLDQIANLNLQLQSNEDQFIKYQEEHKESNKQIEQLLQEKDSLIKSLENQKEKLVEVKNELTEEIEQLNQKIQNYAEGENQRQDWEFQVENEKAVLNNEIEILQQKLDQKESKLNQILQQYSNLEIQYQDKEVLYDSLRKQIDQNYVHIEDYDQLKQKNSNLLNEIHELESNQLKSNSEKQSLRRQVEKLKSDLEMKEQEFEQTFEQMNKRRGDNEDVSKLTKEIQRLNFELSDAQTLLQAKSEQLNIMRRENDEQLEQLNRLKRQLNQASDKELIRKIDRSEHLEENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7R1</accession>
    <name>A0A8S1Q7R1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1480101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7R1"/>
    <dbReference type="OMA" id="RMQEYMV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="78" mass="9459" checksum="A293F1086368CACD" modified="2022-10-12" version="1">MQQRFQKLEQIIIKFSDKHQLLRRGIYVGFIINAGLFYSQMTKFAALKRMQEYMVEKTQQFENRLISEQEQIIKSLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8T2</accession>
    <name>A0A8S1Q8T2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1490132</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8T2"/>
    <dbReference type="OMA" id="IYKMENQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8111972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="212" mass="25513" checksum="8D768365B9ACF288" modified="2022-10-12" version="1">MKQQEDDPKYQQPHEQRINSYMKKPVKLNQLDPEIMKAYSEQIGLNSQQIMPKLSWWQQFMLRPDIRKKYNQYRRFSKETLPMLVFVVFSTGVLIKMEGQYDKMKQKVQNSKTLKEIEIEQEDAYIRAALEGKKINLDVTIQEPRKEQRYKFEDDDDDQNLPGFDTQDDFYEGLAKEAKQGANIPQEYQQQYLEYQFRKKQGDEQQKSKFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QE87</accession>
    <name>A0A8S1QE87_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T1580017</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000163">
        <property type="protein sequence ID" value="CAD8114082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QE87"/>
    <dbReference type="OMA" id="TKGETIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08215">
        <property type="entry name" value="STKc_Nek"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="9"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="285"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="285"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="334"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8114082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="577" mass="66464" checksum="1D16017E7BD8FB28" modified="2022-10-12" version="1">MKVQESEVYKRIKLLGSGAYGKAYVAESIRDGQLCVIKQIDVSFMKQDEIAQAYKEAKIMSTLKHPNIINFREVYKTKKGKLCIVMDYADGGDLAQKIKQTQGSLSESQVLDWFTQLSLAVKYCHDRKILHRDIKTSNVFLTKEGMVKLGDFGIAKILSTTSPCAKSVIGTPYYMAPEMFENQPYGFKQDIWCLGVVLYEMCNKRPPFEGDNIAQLALKVVRCEITPTLEYYSIKLRNLIGKLLSCKEHRRPAINEILREQLIRDRIKSFLSETLQKEQQVIDQSQKAKKSVQQLPQLKRRVATPKQRASPSVHKLPEIKRTIDTPDFSKRVQLKQNQSVLHVQSPKRQSPRRKNQLSEGPETPNKMMKSPSLNIRIQSGKILTDKHLLLKKQIKSFDRQQSGRQIVNHQSNIFFSPKRKQLEEEEKVLDPIEEADSTPLTLTKSNIEFQDFNNSIEERRKKNKIIYKDPFKKDDEMSEMISQLQEIVEDDFDNPPINEQIRNLKARLIQKLGRHFDEVFKQCVQAFKKNGDLGQSEMINILQQRFSGVSIKKVSGIATLILTLIIQTKGETINTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QEQ7</accession>
    <name>A0A8S1QEQ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1590060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000164">
        <property type="protein sequence ID" value="CAD8114328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QEQ7"/>
    <dbReference type="OMA" id="PQFPYYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="79"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="331"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="416" mass="50570" checksum="3036A20E1F7C624A" modified="2022-10-12" version="1">MQKQPLKLIQTPSTYVTKTPEKAINTSPFRGCSFSDKQQMTPLKYNMGYPQVQQTINQNLQPQFPYYENNTSPFRQETKEQLLQRILNLEDNLRVINRKYDQLKEELDKERIRKCETDRSYSQIYQKCQDYEKDLQKQQQMVKGNENKYKQIQKELIEYKEKLSERDNEIIEQKTVQEKFQRILKQKEKEINELKQKLKEEKDFRLCESDRLIQEFSQTYNELSQQNDQLIQENNELKITILEFDNQNRSLSSKKQSEQNFQTNTDFLDDPELRQIIQEYLTNNDEYYLHQIPEKQLRTCIQIIKDIFQTIPSRQLKEIKSQQFQQPKQINEEIEINLQEVRKKREFLIKEIKQKMERIVKQKEFNIMEFNDLQREIEELKKELSSIENLIQQMEQSLLLNPHRNSCVSFDNEEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF72</accession>
    <name>A0A8S1QF72_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1540109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF72"/>
    <dbReference type="OMA" id="MNINRCC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="88" mass="10177" checksum="0DD2DD5922DD5E35" modified="2022-10-12" version="1">MGITCSQPKKSTLTITISQENEDPNYEIKKLLSEERITKLRKTFPSPTKTYESYFLSQINNDKTMNINRCCDEEKFHKSSKLGVPLIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QK73</accession>
    <name>A0A8S1QK73_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1650048</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QK73"/>
    <dbReference type="OMA" id="FADDNYT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002303">
        <property type="entry name" value="Rab_A02 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="173"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8115584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="225" mass="25390" checksum="F64462FF15400423" modified="2022-10-12" version="1">MIKEYDYLFKLVIIGNSGVGKSSLLLRFADDQFSESYLTTIGVDFRFRTLPIDGKNVKLQIWDTAGQERFRTITSAYYKGADGIVMVYDVTQGQSFDDIDKFWLHEVESYGEKNVQLLIIGNKNDLDEQKQIETSKAEEYCKSHNMLFMECSAKTADHVNNAFLELSRKLMAKKDASQPPKTTNTTSNVTQQSQSRGQTNTNTSQSNKLSANTSNQKKQKDGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKC1</accession>
    <name>A0A8S1QKC1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1640032</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000171">
        <property type="protein sequence ID" value="CAD8115401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKC1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097039">
        <property type="term" value="P:protein linear polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20336">
        <property type="entry name" value="Rcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF13">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="113"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8115401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="433" mass="50962" checksum="C0C42A933B42A4CC" modified="2022-10-12" version="1">MNYEQFQDDGDDKIQCDQSSLNIDQQELQLIIDLSQTHGFDIIQSIYAIHFKGFRTIFDAIIYLQNNDSHRQVEQTKDSSGVCKQVSQFNPIFEQDDQKFIAELYKIKYNLQSTQQCCVCYMIFLDKDLLYLKQEKHNICLDCFINYLRQQINEGKINNLKCPHCNIQIMDNDILEHSQELYTKYLKCYQNLEIALNPNKAWCPTVNCQNVLEFKQEQMISTCAYCKIEVCKGCKQRAHPLQSCEENLQHILNEWQEDRETQQCPRCKIIVEKISGCNHMTCQFCLHEWCWICKSDYTSLHFAIFNPFGCPALLPGRIQQKNFSYVKLLIWRLICLILLIILTPIVAVMTAPFLCIALLIQIKCFQNLNIFIQAFLFMITLLFGIALIPITIIIFIITLLPFILGLIVYYYYERRRLEIKHQTALSRHFQLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKC2</accession>
    <name>A0A8S1QKC2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1610120</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1610121</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKC2"/>
    <dbReference type="OMA" id="RIACFEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="213"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="258"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8114857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="55763" checksum="2AE052F4B2523C44" modified="2022-10-12" version="1">MLWTCNSHTMDIEFICLNPNCSETKISCFQCIQNGNHISHLKDVKKIENFYELYSQESNDNTILLNKFGLLIDKIKHQFQIIIDGIQLKYHKTESQYQNMNTEQINHLAFSFIEWKKDMDFIENTISGSINQLLESINLCINTLKLQQNKINSLNIQQEQKILKYELINSIKDDDIFAFAFDNVSSLIIAGYSSSTIKVFEFNQGNLNSLQELNDHNSFIYCLYFMKKSQCFLSGSGDKQIILWSINQNKQFFCKQKLQGHNDSINCLIMSENEELIVSGSSDKTIRFWAKNSQSSWNCQQILSVHKNPVNSISLNQSSTQLISCAQNENFIIISQQQVDRLFWEVISTIQVDQWGYRLCYINNLMFTFQPKSKEQMYIYEIDDKKGQFKKTKELTIKAGHCSMYFPQQYIKSKSILLNKNGNHVNLIRKLENQEFATEQSIDFGTQYIYGTMSQDGDYLITYDDQTREIQIRKIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPS7</accession>
    <name>A0A8S1QPS7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1960009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000205">
        <property type="protein sequence ID" value="CAD8117351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPS7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="57" mass="6752" checksum="0CD39B07BF9DFAC9" modified="2022-10-12" version="1">MTYLQIKRITIVIARKKGADQLVDKLINNFQIFFQIVEIEQLIRLLEVGAYLKKFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QS77</accession>
    <name>A0A8S1QS77_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tubulin/FtsZ GTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1990012</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000208">
        <property type="protein sequence ID" value="CAD8117447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QS77"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="6">
        <location>
            <begin position="3"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8117447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="290" mass="34336" checksum="EF30E1823DD125B5" modified="2022-10-12" version="1">MRKIISLHIGQGGVQLGNSYWELFYLEYGVKYDGLIKKEIKNQEEYGEVFKFFLENKEGNYSPRSLFIDSESKAVQKVKYGANRGLFRQNQFIYGKEQASNNFASGHYQIGKLEQENQLKIVHINKEQFIQFNSGLGALLLERFSDDYSKQIKMGINQYLPISFKGTALVEPYNIIKPLMKFLIRFLILNVLAIPIQTNFQPKFTLHQLLLQDLMNLYFEILQNFKLILFYLVFILCYVLTHLFYQIGKLVFNNKKLMIFQFKYLNHKISCLNVMIYWEDLWLVQSFIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QV79</accession>
    <name>A0A8S1QV79_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PIPK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T2580005</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000267">
        <property type="protein sequence ID" value="CAD8118724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QV79"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00139">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035787521" description="PIPK domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="PIPK" evidence="4">
        <location>
            <begin position="357"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8118724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="711" mass="82279" checksum="2378AC0888458D17" modified="2022-10-12" version="1">MHMINTALILLIITQVKGIYSDSDVVDCNADLSDEELSQAIKLQTLDKYFFLIIPIIAFILTLIIMLSFIIYPKTWKMPGDIIFFISFCEALLCIHWLSTSIYFMINEISPLSDELFCQLNSVVSIFAGTGDVTYNLIFCLYVRITLKEQFVARKKLPLILHSIALFTMISVTLIAKLFNYNGLSLFGTCSLKYNPGFNFAGLVLVFINTFMSVYTMLYIKKAVPENQRYLNLIGKVTRYYSQYITAQFFIQLTQAISYILVGLNCTKFQKGWLLIFITIENSAKLCTPFVLSILRLRDPTIKRQILLIWKQFSSKSSIGISLLPEEQNLVVIAKTLLLDKVNTIVYSIQQIFQQDDYTENNSKVNYLFQKKYKINNQINESQQPLELDAKITQEEINEESQFIIPPEQDLDIELRLMASTIIIYAPKVFLKLRKKDCKVNNYSDSFNPIANQEQINSFTGPDGGKGGAFFFFTFDNKLLIKTISEQELLVIKKNLGSYFFHLSRNESLISPIYGIFKFVLLNGQESQIFIVMRNALQIPSSNVIRTYDLKGSEYQRKVLKQYDLKKDLTKTTLKDIDFKEQEGKLFIPENIKDLLKDNLIKDSKFFSELNLMDYSLLIIKMNWSTFSQNNLQMDINNQYQISKLFNSQLSCIPSIKEKGVYYHIAIIDYLQEWNAEKKIEQVFQLKILKRIQTNLLKKAVNTKIGSQIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QVG4</accession>
    <name>A0A8S1QVG4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T3570001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000366">
        <property type="protein sequence ID" value="CAD8119691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="VEQICTK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8119691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="402" mass="47687" checksum="2DCBBDB6CF26F36A" modified="2022-10-12" version="1" fragment="single">MLSLVNTKISSLRGGGCGSFRMNPRTYENLQSDIQDIDNFITKFSSFVEIICAKADVAINSIESQEIMIAIQWFIFQEENIYKLNKNPQNIMKSYNLIVEGIRKLLKSCQIYIRTDQFKCLYILQITASLSKVIFSFHVMNSDRIMNFDLQKEFLDISDDLKQYIEIEKNDLIQIQMEVYLFLTKTSFEIVPNNSKERQDIVKGFVSGIFDSLIQMKPNAELLKFLFKVVHQMHNIYKIKNNRKQYEVYFQIDMLQWEIIDFFRNEKQLILNEIILQIQLIYDDLVKNSKNWINHFLWIQMIGKILIFNPLLTKQRLNQLMNSNDFGVNSKQMWKEYQKKGLLIKMDHTNDQAVILLSQVQKNKLIELDRQVLEESFKGWEDFLIFKQYFIGELNEGTPFTX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNF6</accession>
    <name>A0A8S1JNF6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0040370</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNF6"/>
    <dbReference type="OMA" id="EPAIIRC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="600"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="640"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="696"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="735"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="823"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="367"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="960"/>
            <end position="1029"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8043147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1145" mass="136304" checksum="1AD87140B1B78B23" modified="2022-10-12" version="1">MQIQIEKKKNSLFTQGVSYVVWKTIVNIFSLYAIVQVSLALFDEQSYKFQNKGTIFLLIRLLIIIVLFADIFIKFNVQQIIDKNVIINKNNEFSKKYLLFIFWFDFLPAFFVLFQTLFYLPNYIAVLIFLKVISLIDADTYTQQQLLHHASIYFVYKVLRLIVILLFVFNCFGCLFYLMGLIGLDSDEQTWLDFSQSYSGIIVKDQSRNTKYIISTYWAVETLTSIGFGDITPVNTYEIFITDLALFGQMFVIVYTVSYIVSLQDQKNPYDKNMHAIKKFMRIKNISSDIVNQVSIFLEQYFYDYKRRDQKSEQDVMRKLTKHLQSKLLYQAYQNQLLKIEWLFNRFSLQCLQDIACKIKELCLGADQIIEQDIYDPKLYIVVNGKINLSLNGVLIKSIEKGGSFGNYEFVTGIRQKSKVAKTETYAILYYISRQDMIQCLQNSFEDYQKFQEIYDQVIYEKKLETIGLYCLLCKSTCHLTQQCQQINIKEMIQDFDQVTIQQRQKFKRSSKRHDTQNAPKDFLQYKFDLKMDYQNLDIDHKCQAYKKMQFIPFVMRMEKTKVDPLRIIRDVIDEANQKKEFKILRALPIINCSSSFVDIWNYFIFINTLILFTLVPLIIFFYIDYDSFKMNPFFKGMRYYYMAILFLDVILKFNINYYFFGALITSRKQIGMRYLSSYFVFDMIPVLILLYFDFIYTYTIVYLLILFKAVSFFQIEKQIQERLKLYPSKYNTYLIFKMFIEVLYLQHFWACMYFAGGEYMYYNYPGTKTWLNDPKSHFGSIITLHYSKQYLVSYYWAVNVITTVGYGDIYPLNNMEWFINDLALISAIMIAAINITNIAFIRASSNQKEQLKNKIAISAFMRQNLISKQTRNFILKYLLNSEIFYDYRNTELEFQTFGMLPKSLKTKLMDQAYSSVYTNLWLNVFDREIIAQIAQETKEAFFSAHENIFLDNVFDNDCSLYFIEKGCIQLFFNLPISNKEETLIAKLKSKESFGHYSFVTGMKRQASARSYKHALLIYLERNNFLKALQHNLKQVALFEQIKYEVLFLNKLQHFKIKCYTCQSSEHMSISCPLTQIKQNILLTFKPKEKMKFIQQREYLKRNKKIKTQRFNQEIDKQFIKNSNKRPFKEVFQQVNFIIDESNQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPT4</accession>
    <name>A0A8S1JPT4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acetyl-coenzyme A transporter 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0050431</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPT4"/>
    <dbReference type="OMA" id="ITFQFIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8043777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="50926" checksum="C65175C5F4029B3E" modified="2022-10-12" version="1">MVFINNLVPLSILYCHQGILLGLSLYSVPSELKEKGVEINTFKFSYLILLPYLFRYILAPLIDSISLQRFGRRRSQLTILYAILGVLLYFSNFQTLNQSTLFWNLFLIFCVLALIDLIIAARLVESLEKEDRGYGAIAQFLGILIGGFIGAYIFSMFNSLDFCNTYIYTNPQEIPVITLQIAFQDLSYISFGLAVACCFIIYNENANNQDIIESYKAAFNAFSNRYLIILFLFFSFFRIGQMPIDLAHLHMKRSSFTEGQIQFLEILAFPIACALPYYMTKMIKTNILEIRLIVVFSIYEIIISGVFIICLFSNEEGLIFPYRDLLLKISLISVWLLNLINITLAYSYIYKNTKSSISATFIALLTTGITFQFIFEGFIEYLLVNYNYYLIWLCGIGIYALFFYQIAARTTKIDQMNSSEFCLEIDVKKERFLISTELTTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQH1</accession>
    <name>A0A8S1JQH1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0060247</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQH1"/>
    <dbReference type="OMA" id="CNCRKQD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="4">
        <location>
            <begin position="125"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8044165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="226" mass="26134" checksum="83C6DC4B9E9B240C" modified="2022-10-12" version="1">MEVLCYKYFQQFIMNHDDTDFKTPQKQQLSKSPITYIKQSSPKKLKEELKKFKIIKKLEFNDDQIVIRQDGILGRSKRITPKENKINSSYTLGLLNSIELNQNNVIVNKLVKPSDDDLFNLSRDENIVCKCRKSKCIKNYCVCSANNQECSFKCECYNCSNKQPKSAVSKQSGSDYIGCNCRKQDCSKGYCECQKRKIKCTSKCNCCDECKNCDIQPLPFHLDSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYS7</accession>
    <name>A0A8S1JYS7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JYS7"/>
    <dbReference type="OMA" id="MRNKNYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="185" mass="21785" checksum="8A6C65B1C55E6C7B" modified="2022-10-12" version="1">MRNKNYQSFEPFKTYQSIKLSTLIQDIHQAGRNLYTSSEHTSNFDSSLPCLKKGEQRKFVNRIYQSYQMEKKAVQQGLLTPQHLNKYLQPRKKHFDLKKQINFLKKTKFQTCIQKLRAANIILNGQKSQQGKNEIIQETLESKKGQQHDIEFIQKMNECQLQQRMGKLLKLKSSPFLFSNQITEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYW3</accession>
    <name>A0A8S1JYW3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JYW3"/>
    <dbReference type="OMA" id="VWIEDIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="277" mass="32829" checksum="B50A1199E6F7C7DA" modified="2022-10-12" version="1">MQKTFAMDVRNIGGLRATADHLYKHQIAVQNAKPSINIKPPKPPVDQKQRQECKQNADDDFNNVLKTFKAVVQTKKIIDNNQPFMKKRTPNNKNKKEKFEQYEHEMNIKAQKRRIGSAGSMQERKKNQFDPVAHPPVFFRREGQNLSNCRLDNLMQYISKEQQQSGEKINLSASIKRWQRSKSSNKKSQIKIQQFVEETEKKDGEFVYDLPKLKGQEKKDYYELKKELIMIIFKHRIYKSDDLEALFGRLLIHNPSLHMVWIEDIFNEIREEFLDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K430</accession>
    <name>A0A8S1K430_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0130276</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K430"/>
    <dbReference type="OMA" id="LLWAQEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH" evidence="2">
        <location>
            <begin position="62"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8049163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="220" mass="25569" checksum="6AA1F69342FC266D" modified="2022-10-12" version="1">MNSTLERISAEEWHYRQKTINISKSFMNQIVLNFFIVEGYRDAAIEFSKEAGIPLSTEELDQMIERIEIKKNILEGDIDSALEKVNKKNNNLILFKLKTQKLIELIKKDQIDQAVKYAQSEIIQLLPNQPHLIEEIEQAISLIAFSDIKKSPMNHLIQNSQRIKVASEVNQHLYQDSMGNDQAKLRTLMKLLLWAQEILKSEIQYPQLLEISKGQFSKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K5B4</accession>
    <name>A0A8S1K5B4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0170073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K5B4"/>
    <dbReference type="OMA" id="ELMISHN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="562"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="519"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8050469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="731" mass="87053" checksum="9E0D03B8F292274B" modified="2022-10-12" version="1">MYQTQTQSQKQIEEMIAYSQSNLSIILNMEQFMKYFRASDPLAIDFLQRNVKQMIDILFVDYDKVQTDQLAIYSDLINKIFEIIDLKLEPIMKQIEIYWEVIFDLRWRALTPDTQWGIAHKLFQLIKDRDKRFVLDQFQRPNFLVSFLPLIHINSVAQILIIFYEFGFHQQQLDFLKVGFDRFQDFDPCSAINFTFIIHEIMIRILNDNQIEYVIKGEIQRKMLAIISRDDINAITLKNAAHILSLVSNYYSMDMQNIQLDEPETQEITQKFRHTEFFEYFDVKIIAQILQQSFQNKTKVGLLIIKLVEVIDNLVRITDIQLWEKVDKANIMELIFNLVHKFNKQDIFISYVDNMISFIFERALNDYHPYWASKLILKNKIHEKHHRLIDKQIYQFESKLSLKLIQDGEFLPYYDYLKQIEEELMISHNWRTLKLHQMKQEEIHKNKLGEDPSSPQIEEKLIVSALDVEDTKYQEQDGIDESANNIFINCQSNSPSKQNYQEITKNIFETIPITRADSESNSESSYNESEQSIEKKFVQDKDFDRPIDPLSQLKEVLSELGMKSNQQNKLSHSDQLDREKVYKNRNLSNSLNHEEQKNKLMTASLKDDYDVPSPILQRRSSLQPVSNVKKLNKKFEEFHIDFLNNRKILKKDDNSILKVDEIDFEKVPSKQIIQVVFQKQNQEILLKTKLVVEPKEEETNLFPKFEKVEDIKFRQLQQKDLHFLEFLKNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6N6</accession>
    <name>A0A8S1K6N6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Phosphatidylserine synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0140013</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6N6"/>
    <dbReference type="OMA" id="FEWINIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106245">
        <property type="term" value="F:L-serine-phosphatidylethanolamine phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006659">
        <property type="term" value="P:phosphatidylserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004277">
        <property type="entry name" value="PSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF7">
        <property type="entry name" value="PHOSPHATIDYLSERINE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03034">
        <property type="entry name" value="PSS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035778470" description="Phosphatidylserine synthase" evidence="15">
        <location>
            <begin position="26"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8049485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="43201" checksum="245788C9056BA057" modified="2022-10-12" version="1">MSFHKLIFWTFTTVLLYIAYNNNEGFEVNSKRGVLAAMLFIAMIGSLKPSDPFGDKYGAIFRIQLWLSATFIAILVYLAFQNEKDGRYLMSLGDNRLGKPVTKDMHTYDDNCEFEWINIVDNLDHYYWSHVINWLLATFLVRDFWFLNFWSVLDEIIELSWQHKLPHFRECWWDHVLLDVLLSNTVAIIIGMIIIKKFKFQQYSWFAKDTFTNPKKFEAVLIVIIVIIGNFLNNFFLMNQLWVPPKCWMVVYRLLIWFMLGHSAFRELHNSITDQNQSYQLRYLTYFTIAIETLISIKFLPDCGNLQNEDTPIYIWLPWLIVGLVSLLWWIKLKLKQSKQQIQPQSQETIQKQDKSRKGKKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6Z5</accession>
    <name>A0A8S1K6Z5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6Z5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="23638" checksum="BF5F2EFC08CD3F82" modified="2022-10-12" version="1">MNQFFSYFHNLINTTLLLYKISNSLTSKTDIQKKKNHIQLFIQVVFFCLILLSSKKKKKNNQLILSPSGLGGNIKPGLFSKHCELNKFNKQNSFKVEETLTPKLFETKESSFAPQPKLQKSQSASRLALPKESKQYRVERQELDNIPNIKHNQMNELSSKLYRELIEFNEQADFGGQNEIQQWSDDMIKKLLIIKIVQNVLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9F1</accession>
    <name>A0A8S1K9F1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9F1"/>
    <dbReference type="OMA" id="EAYMQIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00177">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000044">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF56">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="START" evidence="1">
        <location>
            <begin position="30"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="217" mass="24866" checksum="BF2E78F9C9C73335" modified="2022-10-12" version="1">MIQSEIYDIEAYMQIGQDGYQFLKKIAPLTQENQWQQFSEEDGFKIHIRPDPETGLCLTRGEGFLPYTIDEIFAIIEDIPLRPKYDGICESGQIIRRFSDELMLFYQKFKSMFFVISSRDFVVISRKIVEGDFTYAIGKSLEHPNHPPVKGVVRGDLKIGGWILEKREGGTFCIFVTWADPKGMLPKKAVNIVAGAQGKVVQQIKLLLDERKKNVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB03</accession>
    <name>A0A8S1KB03_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB03"/>
    <dbReference type="OMA" id="NYYVPEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="230" mass="26457" checksum="51E09934B9749AD0" modified="2022-10-12" version="1">MQINGRLTSGDKYPEVNIEQEAAEMASRIMERVRQKSNAKSTQRLSVKPENFRSSDYKPRPSEIQSVREGTISITDRNELKFEPNFNQINPLKNCFKSNNNQIENMKTPQRSQFKSASTGMQDIYQKTIKLNNNNYYVPEHQSQSTTDKPDSTKLIKLTDSVNSLVNKKSDLLSKHKRIESSTQFKLILNAKPHQLQTPVKSNKEDIVSQLLGSLRNQKKYSDYKNSAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB15</accession>
    <name>A0A8S1KB15_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Replication protein A OB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0190133</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB15"/>
    <dbReference type="OMA" id="GKITQYP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A OB" evidence="2">
        <location>
            <begin position="197"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8052339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="332" mass="40055" checksum="5F4BDF2C78FF29B2" modified="2022-10-12" version="1">MKQKRYSNFLDQEEYAQFQYHEGISKSNNQKCSNYYNRISELNNRDQNLYVNRIYNTNNLEKAKGLKDIKRQHQLYQIALKFKKILNEDFIQNQKLVEFKDPYGQQCTCLIGNEVEYPQFKNNEVYYLFGVQIKESINYQKNLQIMIQKGKITQYPPDDQMQQTQQLKQEQKNNSLDINQSNSSLINQLQLFEQPQQNTEIKTYFNILAIVIQQFDRTKILSQKCRKYFDFVKIEVMDQDGYLVSITLWNTFLEYKFTPYQIVFFQNLELKSYKGQQYLQTIDRVSKIIIDDSRLQLLSNYYEVKEAMIKHQENEKFKKLITYQEIQQLNPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDL5</accession>
    <name>A0A8S1KDL5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ISSQICC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1332" mass="157594" checksum="9BF5F3A083B15A7D" modified="2022-10-12" version="1">MNRKEIVENELISIKDNSQKYPQLFKVLESNLLELDTHSLLQQLLPQIVSSKNIKLISLGIGLIQKLQHFTQLTTNDLDIIIEYFNISKQQQDETIDIRLVSTLIQIIGPDIIDFKNSNQILKVLNLLIYFSSSNNSIIAQSSIQGILTISNVLEELLQNKQKNELLQSYFELLELFFKIFQDKEQSFIYVSNNLILCKDIFIIILKCGKYLQQIEQFHTFYHETLYTYLKSINLIQETNIMNKINMIRLIFNYILTIEDKFDLIALLMNTYSKLKPNDQSKIAIHDGILCLYSNLTIFKNLLIRQPVGNSYNDYNDLILRSIYQYCLCGIKEYQTQQSPKQSILINKLTASYIDCNLQMLPNIFVQQIKSLTQIITDNMQKLLQLDEFQNDGNGQLQEIKNYSRLLQSTIFNSENDDQMAEIQETESDDIRLKTFFEKLWRPLLRIMKQILKIANNQQYINLLEYLQSWIQFSLEQDQINSFQLLIRFLAAQSAPSTLKYNETDKWLIACKIFEEILYSNVNLLNAKTWNLIFQTLQRIEQVVTKSTQIKMTNEIFYRSTQYQDATVFQMVDGLNQLALQVSERLQISQKKTFDSIYKSFAIDKLLLIINNNWQRIHLLWTIIDALYFCLCSSKVMEMRLNAIHKYRDTIFSGIEYNKNNKLKWGDNWIQYLLNPLSELITLPYEDLKENILQIISNLIQEHHKYLPQEAFKIFVQLIEVVLNDFFSKEFNHQELNQQEYKSLIFRTQLSIQCICDIISNHIDNLDKSSICKLAQIFIQLNNQNDFNFEITNKIICMTWQVQEKLAQLEIIDNEFQQICLKLYKNCLDNQDDLKYASIHISSQICCIAQDSYFTELFSILEELIQHSMDYFLKIEQHQLFLIQQTPRFIQLPMETPKFQGTIKQMIFDKSTSLQLFKQQFELVKLCIQKIIDVIIHKSLYPKFYYYLQLFNQAQCVYVKHEIVLACQRIIKSNSDKQLLIGSIDFLVTLINYEQTLDLEDVQFYMENKTFEIFCDVIKLTIPINHKKGLYALQLLFNFLCQDQIINILNDDNNIFNYYYKQCLQFDMLEEDIIAEWLHFINQQISQLSIVVKHQYFVEFLINSFEIIINKRQLNKNDLNEANKFVGVLNGLLNKCQKIINDNSEKLQLILMTKLKSIIIKMIPSLDQSLIEQLFEPLITQQLTISNKRTQDWISFYVNDVSQYLNNEQQNVFIQLFTNNYMNKLKISEPETQQLIIISLVGFLDMDNPIIEKFSLSIRDTLLNSIHENLQLFLNEQNISNERQFNIQLMLDFLTRFEKNDLKEFYSIFVKMIKCESLEIRTKLIILLNKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KE61</accession>
    <name>A0A8S1KE61_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0200106</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KE61"/>
    <dbReference type="OMA" id="FLEYGFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049680">
        <property type="entry name" value="FLVCR1-2_SLC49-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 49 MEMBER A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8052997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="421" mass="48524" checksum="01F2E4B887649FDC" modified="2022-10-12" version="1">MKEDRKIQQNNEGFYQWIVLTSFCLNSMSNIFMFTGLSPIWSQVAIYYGTNDYDLNWFPNMYYITIMSFSFLFNPIIIKFFGISQLLSCCLTSMGLWLLLYVKQNYQMGLISFGLIGLGESLYYQVPLHLSKLWFAHDQRILSTFIAQYSNNVGMLIGYTISSSYFYEIVDENEFNVRFENLIFLNAIFATIALVLNLSTLKTPSLRHDIIEIRLTTWESLQKIVMAEESAFDFLSIASFIGLSWCYTMLLGTQQYYLGQTFLQVTQTSIYFQVGQIVAGIYCTWKLQKQSKAGIQQDYDIYIKTMISLGFWLLVFEIILLEDIPFIILVFINFFIGAGLGGLYSVLLEALMEKHFPVQELAIGSVLAAVACAVSITITMTLVIPKFLEYGFQISCYLMIIPFCYIAVFYKTQFKRFEAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KGP5</accession>
    <name>A0A8S1KGP5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0200166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KGP5"/>
    <dbReference type="OMA" id="HIICPDD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045030">
        <property type="entry name" value="LYSM1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932:SF8">
        <property type="entry name" value="LD22649P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932">
        <property type="entry name" value="LYSM AND PUTATIVE PEPTIDOGLYCAN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="1">
        <location>
            <begin position="116"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="241" mass="28597" checksum="61F8121918FAC293" modified="2022-10-12" version="1">MKVGDIPNEVYNRRNQVHRLVEMEEMNLKNNENINQPLVNEESLQQPLKQSQKEPVGCLARIFGMSKSKPQQKEEEYKPTELKDHYDLYDESWFDDQRNIILQSQETPTPDGKKEITHIICPDDTIEGLELQYGVPACRIRTYNNLQTNDIFYLKKLLIPNPTSDYKKQDIDIEKYMQELKIGLFLDQVCQPEDKSRKVAIFYLDMNNWSYQKAALEYIEDYKFELLQKEAKKTSTRKVYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHV0</accession>
    <name>A0A8S1KHV0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0190316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHV0"/>
    <dbReference type="OMA" id="YKQPQVQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="245" mass="29661" checksum="120CB3AD792458C9" modified="2022-10-12" version="1">MSQQFYSKYRHLFEDEMFDQVKLGYDMQMAPPQLQQQQQIPQFKFTQQQPKFQLQQSPIKQESPFKIGQVQNQYKQPQVQSFQPSSQERSSLSRKNFNEDRIRKQVMQDNIFKQSTLEQRQTDRVYQLNQPQQNQHQEQPLQERKQQQQTYNVFQDQDILDDYAPKKQRQQIQTTNDNSQINNIHKVYLQRLNDLDYIRKKYLDPKQLARDIDQNNPYKAALMQRILSQMNGNQRKSKLKEALKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJ42</accession>
    <name>A0A8S1KJ42_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0230205</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJ42"/>
    <dbReference type="OMA" id="FRINGMY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF83">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="468"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="509"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="547"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="645"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="722"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="982"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="342"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="982"/>
            <end position="994"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8055490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1165" mass="138615" checksum="EF83693B4127C6F5" modified="2022-10-12" version="1">MQNHKIATNNDESNCEERQLVDQSKNNEQQQEISRISDSILNAINLNEYDYYNYPPKLEQAEIHRQAYYLCPDQYVLNNYPKDYCECCNKKFHRQPFNWVFGDLYEATVDEYGLAAPLYFTIIKFQLIIFIIVFCIYGIFFISEIHRICNSNPQLNCHIENNDLCRTCEFQYSYGFIDIVTIQEYLHQEKGSNYQWFQVSAFLIFLINLHLPFFYDLLITYIQFKYWNRDPYNQQNETITSIYIRGMSSKLTEKEILNLIKESMQRNNNQIISSQNKLLDNAQIEEIVYIYNTQEIKLLQQKRQNCLLDLVITAQQIKELELSKEIITYKDAEVKVYKAYTKEFLSQRISDLMKQLSEVNDNINKQIIGLQINNISMNNQDINKMRLEPLEFSQKAIIRFNDPNISQLILQNFQISWFNKLRVKLNLIQNPKLIDKLIVKKSFRINGMYWENIGFRSQERIAAKIKSAITMLIACAALMTAYEFIYLYKNDPEYKVEKNNGRLSVAQRVLTNIFTVAVPIITTVAILVVIINQKESKKNTFAHLERSFMHVLIVVNYFLVTFLPYIFTFELWSGKTMSAAVYDLVTLTQNKIITKHVFHTVHIRYMGFVFLRRRIQKNCKEYFQGQLNNLMTPPTFPQRSRVCNAMYSLTVGLCLVYVCPVITIISFIFNCYIYLFDRYSITHLYSIDKKFTISLMRHQLKIYSLSFFPIKIYLFIKLFWRYGWLIYAGVPTCCAFTIISILFREKIVYFLLTKVFRVSRRENSKHTPETYTSNYYQYMKKLNIDEQNEITRKILNSQGCEININRFYKIIIIYFYIQKFPIINLLTIQCNNYLKVIYPQIHSQINQLKKLNIPYKPNKTSWKLFNLNHPSNHNYIKIKIYMKNLFAKSKQRNPIKNIFNGEQIYTQDWMMNKINFNKQQKNQKSTINSQNYNNVLSSPRLVIRATFLLQNKEIKPSSRKRNKENNPQNVWDRLSKLKGTYQTSQSLQLQPQSTGRSQRHTPERVEAISNRPYEEPLQPIKFNFNYQDILVGSPQTSFDILLKKFMKVQQMPKFGLRSFRSKFNQIDLKIEIENDLCLLTIDELAITFDHLSFAPYQAQIIPMQFNEQKSNQYFQKQNKANSQTNELAYKYRARVQSQITDSKPISINYYDHYISNEKRIIKIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLR3</accession>
    <name>A0A8S1KLR3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLR3"/>
    <dbReference type="OMA" id="QNMIIQD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="276"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="469" mass="56013" checksum="7F445237CCC703F8" modified="2022-10-12" version="1">MSKEVRSISAHENIIKYTKLQTQRNYANISKFWPENDYHDKETLYFQIKSLKEEINLIRAENIKLKTRILQQDKELQNFDKYIEELQIKHNPLAKNNEYIIQQIKKQNKDLQLQLQEKLYLIEQMKKNTKVTKVQELKIENQLFKEEIKKLKKLIQEQEKSTQEYQNQLYMIKDNFQKTQQGFIKQQIEINDLKQKQQNDNQKISQLQNEVSKLSQIKINLEDKMQRITTEQNKKSSTNSSKSNKFQATTEISCTQLRSKTQEELQYKLIIRGITYEQFTQMIQELKQQAIDLKKQIEKEDIEYILSQEPFTLGEDRIQEVVKSLSAYGNKLADSLLNQIGKYKTFLDYPDFKIEEAEKVIKQTLKQPEIAEYIQTKQLQIWNKEEVLQMIKYLKITWSESVLLFYILNLFEKSGQILDFKSDSIIDPFLQLVQKESSRIMEESDEDLFDQQSDPSQNMIIQDTDQYIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KME0</accession>
    <name>A0A8S1KME0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KME0"/>
    <dbReference type="OMA" id="GTASTMC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="355" mass="42044" checksum="7A5A241E33C58DEE" modified="2022-10-12" version="1">MNNNEDKKQIERLGTASTMCQTFQQSQFQQQYQKRVDFTQVNLFPKNIKNDLNHKQNTRYSRQEYGNESEDQKQELLQTQHKQQQYIIQQQNSQSKQKKIQIKQQNESQNLIEDRQEEFNSVYDDENLESPIPIQKKGKGEHLYVNCQMQALSEILVKYHIQEINQPYLKKKTSAKCCCFTITTKQSLYQGQKQIIITEGRKNYDENDINHCNLLYSILYQMYQLEKLSKKKSKEIEAKQTTVFQLIYTLSCITLFKDDLLQFTKNDKDKVIGNLFIISKYIFEILLNGILDPIFKQSDSNGETLHNTLTAIHMGMFLMVCLDNLNQEQEIVQNLQNKNIKSLIDLWKQKKYQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRW8</accession>
    <name>A0A8S1KRW8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0260325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRW8"/>
    <dbReference type="OMA" id="CDQVTQV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070728">
        <property type="term" value="F:L-leucine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016684">
        <property type="term" value="F:oxidoreductase activity, acting on peroxide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071233">
        <property type="term" value="P:cellular response to L-leucine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990253">
        <property type="term" value="P:cellular response to leucine starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901031">
        <property type="term" value="P:regulation of response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006730">
        <property type="entry name" value="Sestrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12474">
        <property type="entry name" value="P53 REGULATED PA26 NUCLEAR PROTEIN SESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12474:SF0">
        <property type="entry name" value="SESTRIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04636">
        <property type="entry name" value="PA26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="532" mass="62270" checksum="4DD6EAC0E43B5D1F" modified="2022-10-12" version="1">MEQESQEIRSAFPEYLTNTQEDGEHLDQCLQQFETLINNYKDELNILSAIESKITMVINQTPMQSIIDCYDNIAKDYNLQTKMFRVSKFHDQKYLPQIPILNQYLDSKIKNLPWPIIGDVNSNQLQEQLIKLYNLNYGRIALHEILLLWFPSLIKPYFHVLDSLLNTQTKLSQDEKYYFAIMGAAASGSDQLYDLLIQQFYLNEGDKNWVDLGYESIDEKIKSLNTICKILVQKPWEQSLKNTLQQILKNNKWNKRELIQALMIFIFYNNIGCFSQGNGILKEIDQTLNLQSGNTSISFTLPQEQQQQQQIDLTQNEYIIQELKNQALLDNQNCSSDKIIQVKSKQQSIDEPQEISDLDEEQLILKFKEKFQKYFTNDEIKDSTGYQILRFSEYNFQMNSYPVLKQVYPEGAQKMHDFLLEVKQMTRNTFGKEQQITTAAFRKAIRVYAQNIYRYQHDDIDYSTQMNKLLPKELKAFIKIVSLTPNLLQKKHLDIIPLKLSADEICHITLLTIAAKIEVQLIYLSKALVDCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTN5</accession>
    <name>A0A8S1KTN5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0240124</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTN5"/>
    <dbReference type="OMA" id="CFDDYPE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05580">
        <property type="entry name" value="STKc_PKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="91"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="350"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8056066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="403" mass="47379" checksum="08D6B272939E3CD0" modified="2022-10-12" version="1">MQMSDSEFFADLKYRLDYLKKQELMGVDIHDRNLESSNCESEIDPELDSILTKQGLVKKKQQIQNHNQLSMKLKMLKHNKINFKELKIQDFEILSTLGTGTFGRVRQAKIKGDPENQVYALKILKKTEVVRLNQVEHIKSEKDILTFIEHPFIVKMKTSFQDQFYIYMLFEYIQGGELFSRLRKEGRFANDVCLFYATEILTAIIYMHKMQIVYRDLKPENLLIAKDGHVKITDFGFAKKIGNGKTYTLCGTPEYLAPEIIKGSKVGYGKSVDQWAYGILLFEMLSGYPPFYDNEPIGIYKKILSGLIEFPKFFDNKVKDLIRKLLNPEIQNRLGFNDNGESIKKHKWYRGVDWTRVENRQIPPPWVPYLRSEDDVFWFEKYPDSTDVPKQLPRELQHMFDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYF5</accession>
    <name>A0A8S1KYF5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0300191</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYF5"/>
    <dbReference type="OMA" id="FEECVVC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000983">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45768">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF13-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45768:SF18">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL47-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035879786" description="RING-type domain-containing protein" evidence="13">
        <location>
            <begin position="17"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="386"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8060549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="495" mass="58343" checksum="0BADED338E3C491F" modified="2022-10-12" version="1">MIIYTTIFLYFVQTLANYYGTAQPDIQQIIKLNFTQKVNSLIIKFYKRENDVLCLVSPNDLDQTKLSDLDLTYNPKGFEVYDYQSYQANSENQLIQIVYMAMIYIKCFQLNQNQQQPISLEIYYSENQFVQGCINDCNGYNYPEIIDSICVSQQCHCLEGTFGQYCQFQSVQISNNILTTFTLDAHKWKYFQYQFSSSDINLFCQNQEEELYYSFVLKQNPQLNIPNLKESQKLMSLNNIQQELKKKQTNVYVDIIYIGLYNNQSKSINIQFKIINTDIEEESTFEKNKIVIIVTGCVVGSLLLFAFGLSALKSRQQRQFQQQIQEAIRRNLNQVHNMEQAQSPERQLVQTNHKGFSLRFIKDHFKGHAYEKIIKAYPGLSQFEECVVCLEQMKKANTKLQKICSVTPCFHIFHCMCLEEWLLRQKNCPFCRTEYTRKKIIKDYPWLEVNTIRVNNTDSTYLSRMKNNQENINESQIEFVKQQPSEQLQSNQIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZ20</accession>
    <name>A0A8S1KZ20_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0300298</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZ20"/>
    <dbReference type="OMA" id="PLRIWEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF19">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_3, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="79"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8060759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="132" mass="15964" checksum="BBE7C7C16DE25804" modified="2022-10-12" version="1">MLSIVEERKEQQRQEFVIDEIPLRIWEQVTNQYRQEIMTLENQLWQMDIDKVFQKDNGVPKKLQQKLRMMKMGKSNRECSICCNHFQKDETIIQLPCKHIFHKQCLFSWLDHSTKCPNCRSDVLHALMNQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L426</accession>
    <name>A0A8S1L426_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0330036</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YEMCLGQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000240">
        <property type="entry name" value="ABC transporter B family member 20"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000194">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF16">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY MEMBER 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="44"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="719"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="778"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="845"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="876"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="961"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="48"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="374"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="722"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1058"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1191"/>
            <end position="1212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8062237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1312" mass="146399" checksum="B55A9AB8D8EFE23D" modified="2022-10-12" version="1">MEDEERRLSKVNREENRENRQREGEKQKMIPYFQLFRYAKNRDIVLMVLGSVAAFLNGGAIPSFSLIFGSMVNSFQEAGDEMVRQAGWNAIWFLLVAIATGTLSFTMFATWMIAGERQGIEFRKNYFKAILHQEVGWFDTINPNELNSKVANESFAVQGAIGEKVPTFIMTFSMTFFGFLYGYIWGWQLAIVITATLPVISIITAVFSVIIQQSALATQSAYSEAGAIAEQAINGIKTIKMLDGEDYEHQKYYQLLQSAANKTTKYDFGVGIAIGLIWAASLWSYALGFWYGAKLIADQTYNPNQDSVYTVGDVMTIYFSVVTGGFSLGQAGPCIQNFAKGQAAAATMYEVLDRKPKIYNSPNPKKLLNFNGEIQLKDIKFNYPNRPDQLVLNGLSLKIPPGKKVALVGESGCGKSTVMQLIERFYDCDQGEVLFGGINVKDLDIVDLRSRIGLVGQEPVLFATSIKENLLYGKTDATENEMIDALKKANAWDFVQKMDKGLETYVGIGGGQLSGGQKQRIAIARAILKKPQILLLDEATSALDRTNERLIQETLDEVSQGITTIVIAHRLSTIQNADLIYVIDKGIVIEMGTHQELMNIHGKYEILAKNQVQNQEEEQLSQSQMHIRSKKIILDSLTKPNDTTNTQKIIQMNVADKKNIAEEAVDQFKQLQELDIIVKGQTENTQYDKVADKEQIEVKKKEPDAQMGRLFSYNKSERFQFLLGVLAAMANGCTFPIFSIFLSDMITVLALSNPKNYNDEERSEKMAYVRGEADKNALYFFIIGCCALCLWTIQSFCLSYVGERLTLKLRSDTFRKLLRMPIPFFDEPKNNAGTLTSRLSVDCKLINGLTSSIIGINLANVASLVCGLTIAFTSSWALTLVTLGITPFSFISGVLQAKIMQGFSSQTDEAYKDSGNLIMEAVTNIRTVFSFGNEQIILGIYEKKVQMPLEQATSKGFKAGLAMGFSQMNMFVMNAIIFYVGAVFCRDIDLSVNDMFKTIFSLTFATMGAGNNAAFAGDIGAAKNASKNIFEILDSEDEYQREVRLQKKRLVQSITGDIQLNNLTFKYAGRDKNVFENLSLTIKQGQKVAFVGPSGCGKSTLMQMLMRFYEPDQGIITINGIDINDYDIRYIRRQFAIVSQEPVLFNGTIRENIQYNLTSINMEQIENAAKTANAYDFIIKNQFEEVQVEEKGNEKQRGQGFERQVGPKGTQISGGQKQRIAIARAILRDSNLLLLDEATSALDAASEQLVQDSLNKLMEGKTTVAIAHRISTIKDSDIIYVFKDGKIVEEGNYQSLTNRRGAFYSLEQGISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L538</accession>
    <name>A0A8S1L538_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0310252</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L538"/>
    <dbReference type="OMA" id="NINESYH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="413"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="664"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="413"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="447"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="681"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8061325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="768" mass="91245" checksum="347EDB0DF8CA8409" modified="2022-10-12" version="1">MEHELIWYYIDKSQQEPEKQSKGPLSIRDIDLMIKNQHYYITQLMILIFDTYVFMVQRKVAFIYGQRSKKMFYLRMMDYNKMMNRECKFQIRNHKKTNQRKKLKILILQIYIVLLKHKIKNLQSRSSINIFVKVRVFKDHSQAKVKFSSTYDAELCFTNINGVEFNNNFNVSIENNEVEEQYLVNGQKNRGIVIRIKGKISINECMIIEIYLFNSQIFQQFDFEQGMNRENAIQYRSPQQIPSSKKPNMENLQPHRNTNEFIKQMKKRVDQPPLSVVSQNSQIQDAQSIFIDVSRIEQQSNNSNNSFLDPLKQPILPQCHRSNQPSNCTTQRTNRYEMQRNSKPYLDQTDNMGLLMIQQQMLQLQNENMILKQQLEKQESQRLLFQQQLLQQFQSQQQQQQQQPIVINNNITQSEKQQQQQSKSIPPQKDQEESESPPKKIQRKKQQSQAIQTDKSVQDQSIININESYHNQSQCNKSKQILQQQQIKSHQQQKQSMESLTFGTQLSQRNLISQQSQQELQIQNTDNQSKNQSYQQINTNSIEFQMDNQQKDVQIQNKSDVQRKYSQIDITNSSIQQDKTYSIHNPEIRKQSQPSVKLEFNFEEGTNESSKKCLADYFSQRKKTANVERRVSVQMPIQEEDQSWIVNVKQRSKEEQIKLRKEMMEYGRSKRSQNKSQTPINDDKNSGQKEQVKRVQSPLMERLAMGQKAKVEEKEMFALTKKNYENLPEVKSKKNQEEVIKQKQLFMKERQNKLKELDDKIKEKMKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L8Z9</accession>
    <name>A0A8S1L8Z9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamine synthetase</fullName>
            <ecNumber evidence="3">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0320325</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8062093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L8Z9"/>
    <dbReference type="OMA" id="CAIGANK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000011">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF57">
        <property type="entry name" value="GLUTAMINE SYNTHETASE 2 CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="10">
        <location>
            <begin position="25"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="11">
        <location>
            <begin position="113"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8062093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="398" mass="45841" checksum="9CC69EC3E3551FB5" modified="2022-10-12" version="1">MTETIENLRDFNYYQLSKAVNKTVVLAEYIWIDGTGESLRSKTKVYQTPIKTIEDLEWWTYDGSSTDQAVTRFSEIYLKPVRLIKDPFRGDPHILVLCETYLPDKKTPARYNFRWIANQIMEQAKDHKPWFGIEQEYFLLKRTGTTHLWPLGWPTGGFPYPQGRYYCSIGERNNFGRALAEAHLRACLNAGIKIAGLNAEVAPSQWEFQIGIAEGIEIGDHMWLARYILERIGEEFGIDINYDPKPILGDWNGSGAHCNYSTVTTRSEGGYRFIVDKLMPILKENHLEMIKLYGQKNELRLTGRHETGKYDQFTWGDGSRGCSVRVPIITKEQGQGYFEDRRPAANIDPYLVSAALVDVTCLNCEHLKQLNSIFEDSLKPLGQQIFQQQKEQFQQQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LA30</accession>
    <name>A0A8S1LA30_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0340116</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LA30"/>
    <dbReference type="OMA" id="IEWRDED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14008">
        <property type="entry name" value="STKc_LKB1_CaMKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001580">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001152">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF77">
        <property type="entry name" value="SERINE THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="71"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="15"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8063035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="425" mass="49045" checksum="2C27A9BC1E50CEC2" modified="2022-10-12" version="1">MGCVCEKSKRVQPEPTQIEVHPCETAQPQSQPQKQTSLKKLKIPQKKKKVRETQKLVKTTNEDGVKMINDYIFDEFLGEGAFGKVKLAFKRSSGQKYAIKIMRKSKLKRQREYIKDAKGNMVIKDALQDVRREIAIMKKLRHKNLIQLFEVIDNPNNDKLFMVLEYADGGQVIEWDDDECKFYQVNESVILDEPLLNQIFRDCIKGLNYLHKNGVVHRDLKPQNVLLSENKTAKIADFGVSTMVGSKNDVLDNTQGTYYFMPPEACDKDRVKDGYSGKSADIWALGITFFAFVYLDVPFTGSSIPDILHNIANNEITFPQRNDISDGLKEFLQFILQKDPKNRPKIPEIAKHPWLNAQNMNLWDEINKEEQTDMEIAQTDIDNAYSLSSIMMIKNWAFKWRTTSNLKKITNIQQSQQINSEQIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBT4</accession>
    <name>A0A8S1LBT4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0350108</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LBT4"/>
    <dbReference type="OMA" id="SENEGPH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05580">
        <property type="entry name" value="STKc_PKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="91"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="350"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8063725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="403" mass="47331" checksum="824054394379888F" modified="2022-10-12" version="1">MQMSDSEFFADLKQRLDYLKKQELMGVDIHDRNLESSYCESEIDPELDSILTKQGLVKKKQQIQNQNQLSMKLKMLKNIKINFKELRIQDFEILSTLGTGTFGRVRQAKIKGDPENKVYALKILKKTEVVRLNQVEHIKSEKDILTFIEHPFIVKMKTSFQDQFYIYMLFEYIQGGELFSRLRKEGRFANDVCLFYATEILTAIIYMHKMQIVYRDLKPENLLIAKDGHVKITDFGFAKKIGNGKTYTLCGTPEYLAPEIIKGSKVGYGKSVDLWAYGILLFEMLSGYPPFYDNEPIGIYKKILSGLIEFPKFFDNKVKDLIRKLLNPDIQNRLGFNDNGESIKKHKWYRGVDWTRVENRQIPPPWVPYLRSEDDVFWFEKYPDSTDAPKQLPRELQHMFDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LG28</accession>
    <name>A0A8S1LG28_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8064972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LG28"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8064972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="78" mass="9818" checksum="F5AB10B956F60BF2" modified="2022-10-12" version="1">MHLERFQQKQKYQQLQQVMDQMKYNLAYKEQKLELRNQYHNDLNQFYINKSNMPRVQNFNIQNQMRLLKKIQQVENQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHB1</accession>
    <name>A0A8S1LHB1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0400160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHB1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="61" mass="7291" checksum="2DBF8338833091ED" modified="2022-10-12" version="1">MPVAPPEIQFVSNEYQFMENWSLPINFYGCLRIVYVQLSPFNTMMCLIYKKPLDFAKRHET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIM0</accession>
    <name>A0A8S1LIM0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIM0"/>
    <dbReference type="OMA" id="GNYCISE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="462"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="491"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="521"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="65457" checksum="D3802DD64CA02910" modified="2022-10-12" version="1">MNNSQRGVENRPNSQQNSQDIFQQEIQINQISQNNNNTVNQLIQVLNNQTNLPIDQFKIVVDSVVIDKQFLLLTLNPKGKYFQTLRKLILLIVGFLTLQFYQLLYLFTQDQIIQGHDDNHSKLILRPQEHIKNKNSSRSHLYLEEQIDQLILVIAIRIIYIVISYIIVYGIKSLSLNYSNKNLLLFFLLLISGTYFIQGTQCNLYPNYGSLHFEDIGKINKTKIYLDNLIFFGCFFVQGMSSASIFYLLMEQVQFMLKKNQRLIFYNEFLLIIVEFIVVSSVLHMIFKEQYIFNLIIMWIPFSLITLFAYFTLQDSPLCILNRISHYEEISKQNQQNLKKQQIALNKIEILSTEFYQNMNSIYSANFPNRNYFNEEQISLLINKFQFKVINKQESKSTINFIMFALSLLYFGHLFKLYAFINQLQWTIETLALILSIFELLGYFIGNYCISELYNEHLLRKLLMTFGLINFLSSLPSFQSIHSQFQSQVEFFSILIVRLLFSLAIKAFLIQFSDYKLNFGFLVLGILLANINLLQIQLFQVVIATILLLAYHLSKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKJ4</accession>
    <name>A0A8S1LKJ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0400059</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8066995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LKJ4"/>
    <dbReference type="OMA" id="LQFEAIW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8066995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="485" mass="55618" checksum="451A2BA0C07A6A6B" modified="2022-10-12" version="1">MSDFKKTLNSSKKQQDFQLLKLIPDSLPDEIKQALKSSVENLTNQEADSEKFEEYVYKVNSDDIITSFIGLNRCRKLLSNPQIAAPTEQMIELFFQTRVIIKIFDIAKNTNIPLLKYEALWIICNIGIGTQKQIQTIVDNDGINILFQALESEYVEIIELGVWALANMAGENLIFRDMLLQQGVLKLFINLANKCRGNQSETNDWGTKNLTILKTLVWAFSNLAKRRKTQEKIFYKDLVYILCQIIVETEDEEILNDACWGLSYLSHEDSIMHIIIQEQITQKLVVLLQKENHSIVIPALRILGNILTGNEEQTNVVLNCGVLQVFQNLLQNKKRTLRQEVCWSLSNIAAGSSGQVKQIIRDDQLLNSIFLLLNNETSNVIEQISFIFSNCIFSAELEDIDYLVMQHGLIQKMSLLLDMNEKIVIGVTLEGIYKILKRFQNDPRLEQYKKLIVDSKIMDKAANQLKYSSKEISFKASKVFEIFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LLJ0</accession>
    <name>A0A8S1LLJ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0430011</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8068810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LLJ0"/>
    <dbReference type="OMA" id="NCATIVA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904430">
        <property type="term" value="P:negative regulation of t-circle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010569">
        <property type="term" value="P:regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090657">
        <property type="term" value="P:telomeric loop disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001352">
        <property type="entry name" value="DNA repair helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002687">
        <property type="entry name" value="Helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF34">
        <property type="entry name" value="REGULATOR OF TELOMERE ELONGATION HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="53"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="802"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8068810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="906" mass="106601" checksum="13697109AFE2C12F" modified="2022-10-12" version="1">MKTPFGLQQYFATKQAQHHQLLDEESEHSHKMLIKEIEIDANMCKKQASLILDNTPVYFPHQPYDVQKSYMESVIRALNKKQNALLESPTGTGKTLSLLCASLAWLKKNRKDQLNSDQPKNIKIIYSSRTHAQLKQVAMELKKTVYKPNVSMLGSRDQYCIRGDFSTIKGTLLNQSCRKSVKSNQCQFYKKDHLILMAQNYSTLISSLEEAKQFGYKNKLCPYYFERQRLDFADLILLPYNYLLEKDFQDVVEIENSILIFDEAHNVQSTAEDGSSFLITHNNIIEAEKDLEKWIDELESISIFYDQLKAKLNSAKVSSELKEFRSIMITIRVFAQYLESFKNNQLQFINSDKEEKYLILDARQIQSMIFENTQDKENTFRLWTENSQTNYAKGVNKNNFAKYLIHCSILIEIMGELSQIPGYHFESWVKFMKNVYDLIKVEDEREKQKLSLNSLQNEFNQYKLSFILDQSNQLSINMWCLEPSLAFSRLFNKSIYSVLLTSGTLSPMPSWACELRIPFDVQLVNEHIIDLNKNLRVFQHKAFDFSFNQRNNEEQISKFGVTLLSLCQIIPNGILVIFSSYSLMNKFRSKWTYNKLLTRLCEIKACLWEPQQSAEMQNIFDTYKQKAKKGAIMFAVHRGKVAEGIDFSDELCRAIFLVGVPYPPKQDNHLLEKMGYLDKIFNDPEFTNQQRIRSSEWYTQQAIRATNQAMGRVIRHINDYGIVYLCDKRFEYKDIKQGLSKWAQPAIQPWINDDEIIKHTKEFYNRTISIKEQCIKEQIEQKPIQEQDCKKRKLQFFGLQNKNDFEKLKDETQDRMKNLNEQSKQNEYINLQSYQMNHHLQEQQFKNSNKNQQKDLEILNSQNINFSNLDRNSIQQTQLKMDIDDDQISRIESKKKKLCIRLKNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQK1</accession>
    <name>A0A8S1LQK1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0430222</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQK1"/>
    <dbReference type="OMA" id="EYFTIYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="119"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="870"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="748"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="761"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8069149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1033" mass="120630" checksum="65B8641116D7E09B" modified="2022-10-12" version="1">MLYNFKEYFTIYQEHLRNRDQWRLLMVLISIYQMCDVIIVTGDAITLKIQGDKYILIYRILTQIFLIAFFEVLLLKLIKIKNGFINLVTLLKLICVVICWEELDYYAEQQIFQRILDKFLILVFFVFAIESQVCQTIAIIFNLIYTLLRNFEFDSKLNIIFGIKLIVFHIILQFLMIMFNKKNIRNNLNSQLIAMGSNNKLIYTQLNSQRISNPNRTLHQLYIDIPENRNISQIPQEQKQEDQQTESVQQDQINEAILSSAELGIYLVDSQTKTVQVINPQLSNIIMKEDQLSTQFLETELYDFGLLLEESCLLLPKFHRSQDIGQFLTFTQNLEYYIIPSILDNNIESEPSQIKRLQLLTKKISFKVFFDNLIAYNHLKYSNNHSLIDFSLKIYIKFDTCYLQVVLSPLIYKLKPQIAIFVSDITEDPYITQLLNATKNNDFLINDISQKIKQPLNCTISMLEIIMNTTPQEIKEKYISPALAGCKLLINTANDILDYAQLQKKDKLDLVQMDVQIQEFLTDIINVVKSQAIFRGLKISINVKQNVPQFIRTDPNRLRQILLNLLVTSIQATVRGSIIFCVSKSQMLNEHIDFVVKVKANETNFSILKIIERTVRFFKSQTLKSKILDLGNLRQYSQSILISFYLTKCLSQIPFEYNYERVGNKEEFSFVIKIQNLYPQFNQNLNQKRLSEFTNQQSFYQQYQNKEGLKRYQSQMSSLQPEDPNIKIELEEARKKLNINIIQTQGQVSKEQQKEIQEQSNESDESLSDLQDDEVSIESKNGISNRVEQMLKIKPYFFDYVNETQQKHEGSSSQPSQQLTFGGLGQGRSSIYSSLCFTSGTMQPNDLLDCFEKMEKIKQQKNKSNCKCTKILICESIDLDLYALSHQLTNIGITYEYITQRSQIVSTLVKLLNSDLQQQQNSNQQKQNIDNQQLKIINDNQQLKNNENQPCCKGLQLIFIAIEQFDEELYALFNQIKDVYAEFKAEPRIIGLIGCMDEENRAQLRRLPFHDYLSKPIMIDALLFILAKWIKMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSI7</accession>
    <name>A0A8S1LSI7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0470166</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="AIANICF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14270">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23810">
        <property type="entry name" value="UBCc_BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001419">
        <property type="entry name" value="ThiF family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2660:FF:000005">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000146">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF4">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBA" evidence="10">
        <location>
            <begin position="452"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="12">
        <location>
            <begin position="492"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="11">
        <location>
            <begin position="3372"/>
            <end position="3539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="3295"/>
            <end position="3315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="4396"/>
            <end position="4426"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="757"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="4396"/>
            <end position="4411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8071408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="4650" mass="540269" checksum="4D743575E955AD7A" modified="2022-10-12" version="1">[encoded data, 4650 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LT11</accession>
    <name>A0A8S1LT11_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0460083</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-13 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LT11"/>
    <dbReference type="OMA" id="NNYFPTH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01367">
        <property type="entry name" value="KISc_KIF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000012">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971">
        <property type="entry name" value="KINESIN-RELATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="11">
        <location>
            <begin position="7"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8070677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="632" mass="73930" checksum="5D83A577610D336C" modified="2022-10-12" version="1">MMDIFDRIVVAIRKRPLSQKEIIKKEEDIIIVQNDNSVIVKEIKQKVDLTKYIEEHQFNFDLAFNQNHSNEQVYINAVRPIIRAAFQRAKVTCFAYGQTGSGKTYTMIGDMERQVPGMYLLAGQDIFQIIEMEEYAHLQVYVSFFEIYCGKLFDLLSQRNQIQIREDAKGNVNMINLMEKKINSVQQLMNCIQLGQNVRITASNSSNSESSRSHAILQVILKSGKQIHGKMSFIDLAGSERGADVQDQNKQTRIDGAEINKSLLALKECIRALDLNKNHTPFRGSKLTLVLKDSLTGNCKTVMIGNISPSSQSSEHTLNTLRYADRVKELKKPENRLFQGDLMQRELMLARQTKNITRKQFNEEDDQENIRNFSPISNIKRQSLQPLHQSTQMFNNQQFNQQKEIRKSDISSAINKFNLSSIPSINVNFNKTQQINSNNYFPTHQRINSESTYQNDYDHFNNNQNNQFLKTQNSFQQQNQLFQQHQFNHTASYSELFSESPYQSQQEQGFRQLNQQAIQQNQELEEEFEYQNPQKSYRQGHNNYSSIVQDHKNLIDKIIEISKLEMKEITMYENQNDMQSYLQTISQQLQQKVELIQEFQQKVCELQQQSQHWTFQPNDSQNRDDLFQYTNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTU9</accession>
    <name>A0A8S1LTU9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0430183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTU9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8069071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="75" mass="9190" checksum="74B8F60FF01EDF91" modified="2022-10-12" version="1">MIIYYNNCYYVDQLIISNYTQARRIFNVDVKGRMFQGCILIDWCLYLQYFLEKILIKIRIILFRHIQCIKDDKDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXC5</accession>
    <name>A0A8S1LXC5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0440030</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXC5"/>
    <dbReference type="OMA" id="FNCPVKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16650">
        <property type="entry name" value="SP-RING_PIAS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF4">
        <property type="entry name" value="TONALLI, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="6">
        <location>
            <begin position="403"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="139"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8069446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="531" mass="62847" checksum="D4B34D8CFE42300C" modified="2022-10-12" version="1">MNQNNQLQEEITKELLTIKQDIELKIPEFIRQGCLELKVDQISLQKKQDIIKCLGGNKYKQDNQFIPKLTECLLSHLLRMKKCSLQSTNINPARPIYLGQLITITMFYYEDKIIKSYSILEELTKFVNKQIHVYFEGIRERLKIIKDKEQQLLNQLRNSQSNNDKQNETQQDINFLKCYCHSQPGLYVQIKTKLNTSKILKCKICSQNFHGYCLQINNDIQDFICPYCTLVMLNPQNKVIDQIVQSTFQQFLQYKNEKHFLFNCPVKHKGSQIEIRCLRIDGKDGLNEITWPDYGELQMNGVKLAEFKPLSINYSVKKRKDDSINITNHIKHNEQNRITLIEYKSNEDLKKQFRIQHQCIYFLGIFSINQLNAKEFLLDVKQYHKNYLSIEDSFKLFKQECSTNKDVKIKSIRISLLCPITLQLINIPARGRFCNHLQCFDLENFITAIDDQKDKKIWKCPICKLKCFKFLIDDYQQVILELISENSLSNKEVEFKENGEITDMILRELCNQRLKNQYQINDRIKKQIKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LY93</accession>
    <name>A0A8S1LY93_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0410086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LY93"/>
    <dbReference type="OMA" id="FVQQIDP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="56458" checksum="CEBE2E0F0A51CCF2" modified="2022-10-12" version="1">MKILRTADFFGAPFVQQIDPKQSIYKSAFGGLVTLLICSASLAYAIWVLYKWQNNQLSPKISNSMYVSDFSLLDLNYDVIKLYYWKFGENQIDPFESKILLPLVMYTTNFSFTELQLIQMSNETSDDGTLYYLPKMSLAFQNISGVIYTSSEMYIQIAKCSQIYLKEDEKCASDELIEEFFSQPLNTIVLQVHQKSLNSLDGSVQNSVQEFFIQIEQQNCYTLNTFLQSNLYELQDSFLFGYPRYLEFINGALIQPQTNSVQYCKLAYENDILGLIYIVMKGNQIKTIFEYPHAGDLLANIGSIVSVLFMIKHIIIIINEYYLNQKILNQLIQFYYPEFKKVTIQKNWRQKTVKVVLNKYEVDVKEFQQFHKKVQDQMQNKLSYLNLLYEMSRIYFIIRSFKFRDELKKSHQVGIKINLSQSKMMADSNLNSNWTNRSSQFFSQLNDEDADLLSLSNKAKEVQLSVPDEICIEKDFYENNKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYB7</accession>
    <name>A0A8S1LYB7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0490168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYB7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="47" mass="5308" checksum="B625C133DCB5653D" modified="2022-10-12" version="1">MCGILKGVLNASGFTCQVSYQFKTDERGVSYYPHTVFILSFEDARDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYK1</accession>
    <name>A0A8S1LYK1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYK1"/>
    <dbReference type="OMA" id="CPSRITN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="294" mass="34313" checksum="81EA2DC32A405B40" modified="2022-10-12" version="1">MQLKSVHQYYIKAKNQSKDQKGTKCKEQPQATASQISLILSKMNNKNVQDSKAISDRTKNNIQISPKLQNCLSASNLMSKTTQSPKLSECQLMNKKFAKECPSRITNGNEIQLPTEINSFLEMEERKRSLNNFKEQLSQNIMKIQHLDQLQTQVKLIEQQLHSIVQTIIQLKDSLCMQLQQEQLTYTQITQSISDSLYKMKDDINHNEVNIIDSMQMKPFQNIMTIYKKRLDEHQSCVQQMISKRQFVIQFNQWCQQLMLKLQIDTKSNFDSDQKENCFYGSNKIAESPKFRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4M0</accession>
    <name>A0A8S1M4M0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">T-complex protein 1 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CCT-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0470230</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4M0"/>
    <dbReference type="OMA" id="RGPNDYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03335">
        <property type="entry name" value="TCP1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000009">
        <property type="entry name" value="T-complex protein 1 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000070">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012715">
        <property type="entry name" value="Chap_CCT_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02340">
        <property type="entry name" value="chap_CCT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8071536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="545" mass="59571" checksum="167A48F7B6A03254" modified="2022-10-12" version="1">MSAVGIRGERDQGQDVRTSNVTAVMAIANVVKTSLGPQGLDKMLVDEIGDVVITNDGATILKQLEIEHPAAKVIVELSQLQDKEVGDGTTSVVVLAAELLKRANELIKIKVHPTTIISGYKLAARQAVKYIQSHLVHKITEDDTEILINAAKTSMNSKVIGPESHIFAKLAVDAVRLIKTQGAISGKPKYPIQSINIVKSHGQSSNQSELIKGYVLQLQRASQQMVTKVKNAKIACLDINLNKFKMQMGVQILVDDPNNLEKIRKKEMDVLKERIHLLLSAGANVILTSKGMDDLANKYLVEAGAIGLRRVPKDHLRRIAKATGAKVITTFANEETGESFDVSSLGEAEEVYEEAIGDNDYIFFKGMKKEQSASIILRGANEMMTDEIERSVHDSLCVVKRTLESGSVVAGGGAVEMALSIYLDDYARTLDTTEQIAVAEFAEALTCIPKILATNAAKDSIDLISKLRVLHSKSQSLQVDEKGFKYSGLDLVKGEVRHNLRHGVLEPTVSKVKALKFATEAAITILRIDDMIKLEPKKEQMPGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4U3</accession>
    <name>A0A8S1M4U3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0510202</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4U3"/>
    <dbReference type="OMA" id="EQQKNTY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="3">
        <location>
            <begin position="349"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8073712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="549" mass="65214" checksum="B683831F6E43DCC7" modified="2022-10-12" version="1">MLSDLFPFYKDKKENSYMLFGKQNNKDHADMFTIFRTQLKHQFRNIINHFLNKLQLKQDAELIIKQNLSSFYIWKNQNQEVEVYYIDEVLTSEIKYKNQIQDDTYKQFYGILLSLSNLNIQDVQQEITTANFYKEIDQLYHNIAMVIQKQLKIGNTDQQFTAIKIQTRNTFILRPSGDIIKLPKINNDIYNFYHTNIYISTRIPFCNPVQGEIDQKVFKYIGSIDQQTYLPNGQGILDCEGAFQFKCKFVDGLATGKGTLDLKLQKQKFEGNFQYGLKHGQSITTNPNQTITIGYYQNNMKQGLFITKKQNGSLLQETYFQNDQIVKKYDYKFDENMISTRYLVNHKDISINNHFFTGLTEDKWINQLLIDYYLCLLTDYYKNISGEQIYLFNTTQSQDLFTSQISKLDHKSVKLQSKYEYIINEYQKYNKIIFILNADQAHFLVVVYQNKSLFMLNSYVSQNDKKILSIIASIFPIKLNLQYVEVEQQKNTYDCSLYSIYNVMMQYKYYNVDVDKIDYRVSFKYIKKLRLHLKNIISNDYAHIILYQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M7Z6</accession>
    <name>A0A8S1M7Z6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0520009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8073961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M7Z6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="24312" checksum="43342A3A2A3F461A" modified="2022-10-12" version="1">MEQYIFCYHLLLINISIIIINEICKNKIQDILNSILKILFPIIIISKMFLRHLFLNIGILEQKYLLDVQLINAQKNKRRELIQMFLWSRGIFKESMNLFKLQLQNEAIMNKRIKRLRVFRILQFGAFLQLADEQYQAMQPQIKADDDISFFKSNSTIYFLGVDLFYYMKDKDNFKKCSVEILGLFGNIVAQPDKYDYELLYGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M812</accession>
    <name>A0A8S1M812_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0550146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M812"/>
    <dbReference type="OMA" id="HAIFELE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="146" mass="17059" checksum="B74656CF2BD55412" modified="2022-10-12" version="1">MNPNPPPVIVLYKQSLNDPDKKNYSKLWQQEPIEVKEFFSNLFQALKYSKDGMTHTIFELETSDSLSEISEIDWKIQGFISEQSNSMQELLNILDIEKISFQSAFLENNSINSIAQLCERMKTLNINEELSHRINDSFLKISKLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M877</accession>
    <name>A0A8S1M877_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0560145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M877"/>
    <dbReference type="OMA" id="TRWIQIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="220" mass="26135" checksum="83C20543EBAA5854" modified="2022-10-12" version="1">MNLTPTSQAIFQSFFIQKTKLQSSNYVQNKSSQPNKLGCSKTKQLYERPCSTIIQQIGSVDYSQTRLDRTRKISLNESQLTVRGIRRPSKILENSEYNQQVPQLSQYQQRRTRWMQGSEKQSSPFKQINSPEQEKEIKNIKRIKLQLLIKDNKYPNFKLNLMKKKLLNQSRVDEYFNDMLFHQQKNNPELSDQIRIIITRQEQTLEPLDSSRIEKIRNQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MB96</accession>
    <name>A0A8S1MB96_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0510282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MB96"/>
    <dbReference type="OMA" id="FDSHCNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040002">
        <property type="entry name" value="Sm-like_LSM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13110">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="6"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="85" mass="9619" checksum="835AAAEA5312C395" modified="2022-10-12" version="1">MDTLDEPLDVLKFSIAQQIYIKMRNNIELKGTLVSYDNHLNMIISKAEETSIQEGIKTKRKLDALYLRGDGIILISPLKKGITSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDT8</accession>
    <name>A0A8S1MDT8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MORN motif protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0550021</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDT8"/>
    <dbReference type="OMA" id="MIGEYQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048678">
        <property type="term" value="P:response to axon injury"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052315">
        <property type="entry name" value="MORN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46614">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46614:SF1">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="294"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="294"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8075715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="42569" checksum="378BB9334267577E" modified="2022-10-12" version="1">MKVVQFNNGLYYGQLDYFYKKIGLGVYIYDDSCLYFGQWKNDLYNGEGLIIFPFGGILKATFKNHSIEGIGILITNNEVMIGEYQNSVLINKALLFNGIQWKLRSFLKNGQYEDYDCQPSKEQKLFSEFIKQYPQLYKSKLLLSLVGFIINDNQMYIGEFKNNKLDGLGRLLDFDKRILEDGYYKNGLLQKGFRYYVENNSFEQVQLVFEQGSFDYKIIMSGMNFPSELIQKQRALIHLTSKQYISKSQEIHYFHNRYQKCNNQIKPLLKITLNQFIFDEDVSNEGETNKPIEHVRKASFHNEKNKQLDEQTTSRTSSFHEKSRQFELDMCKIEFENALKPLPINIKQPQRKRIFDKYNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MI55</accession>
    <name>A0A8S1MI55_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0620216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MI55"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="101" mass="11998" checksum="7658D03D0BA21E7B" modified="2022-10-12" version="1">MNPNFDNSFHQKDDIIQKFPQSEQCLSNHKNFSLDNDIMRTYSLDTPKVNFENWNTQTQTKVIKQECNLDNPFIQNQNVESEFQINVDNILQINQNCIKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MI96</accession>
    <name>A0A8S1MI96_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0630151</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MI96"/>
    <dbReference type="OMA" id="NIKMQPR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF147">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="462"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8080217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="590" mass="69364" checksum="341D60B08C0F6F24" modified="2022-10-12" version="1">MKQQQVKEPHFNGEFEDSESENEDNSKKKTTLIMNVSDTQYDVVKFVGKKLFKWILQYESDVTNWDMFWTDAAVQPETLGKMQPYQKINHFPGMYSLARKNHLGRNLMKMRKQFTDEFKFFPQTWLLPAEYGDFKNQFVKGKAKTFIIKPEASCQGRGIFLTRNINDINPNDHYVAQRYLHRPFLIEGLKFDLRVYVLLAGTDPMRIYVYQDGLVRFATEPYVPPNSSNLEDMCMHLTNYAINKENPNFVFNKDASRMDIGHKRSIKAVFSKLEQEGHDIKKLWQDMYELFIKTFCTVQPILSHHYRSCQPDNYANNMCFEILGFDIFLNHKLQPILLEVNHTPSFTTDTPLDALIKKNLIRDTLKLMNVSLKAKEQIIASRKESLQQRVLTGKKVKLTMEEKISQMKQWEKQRNDYENAHLGDYVKIYPLEDYQKYDKYIEFASSLQDSWTGTNIKRNQKKDLKDSQIAQQQQQQQQTKNNIIRAPFKPNTPVNNLPKLQNILRPESINVHGEDEQVGAKVSKLKNIKNQPRQQSEPPFFRVKQAIKKQPQQMIPKPCLQPKLFDMESIGQDKFNNNKKQLYQQLMLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJG0</accession>
    <name>A0A8S1MJG0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0600157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJG0"/>
    <dbReference type="OMA" id="QKIMGGA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8078601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="177" mass="21043" checksum="09EAB2425AC996F2" modified="2022-10-12" version="1">MNQQQQELRIIKLKIEKEVVQIDQRFANVSSFFQEIFEKEPDCDEIIEIPQSCVTQKAFDYIKKYYEYNKYEPQKIMGGALNADQLFLNQHDKELMLPVNPFNGDLLKQLIQAAVYFQLEAFKKLCLARLYYEFLIDPTDSKWLQKLAAKYPEVPPLSIAHLEQYKTLYPNLFKEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKG1</accession>
    <name>A0A8S1MKG1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0640181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKG1"/>
    <dbReference type="OMA" id="HKQKSAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="340" mass="40093" checksum="D65F771F7263664D" modified="2022-10-12" version="1">MNKGKNNHRRAQSNNDFQQIMNSKIVGLVEQNSHKNLHKNQSFGNMLFQIPEQTDIGSSTEQSHHSEKKNCINDCVLSNKKNYSQDQKENLINSNSCTNIISSIQYMIKSSHPIKEIAQKTLNESQNKKKHLSFTNELQKKNSQILHQSNKIDISSSLTPDRKKTDNNNSKQNSQKQKSAQQQIDGQEKQKIELSNFLLNNLLKVDQKKKINIEQQDSFRKMEYQMNEIQKDISKIKQRQDELDLFNRNIQNQLFDFISESRKYQDYGYLSLQKLEQLEQITRRNEESIKILKQKLFNNEIQQITTKQHENNINFGNISSYKQELDIQKKLSDFKYIKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MND3</accession>
    <name>A0A8S1MND3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0600041</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MND3"/>
    <dbReference type="OMA" id="EFSESMC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10019">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="6">
        <location>
            <begin position="11"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8078426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="386" mass="45781" checksum="C9ABCB67A6615C98" modified="2022-10-12" version="1">MQIQKDLKSRIQEILQKHNIKQVDLAREIGIHHSTLSLWMQNKNKPRKQDISNSQSTRIEELLERWIATFHANKGQGMGTGQFRLPDSRASQFLSHHTVRPVLPISKFHILRNQYQNSIKQQRQEIILRKTQEQLKNNPNISFQQALNQSAQDIPIEPPKHQQLVPITIDFELEGKKIYQTFCWNLNESHMIPETFARIITEENQLPSVIEQEITTQIKKQVQNYKCYYPTKNEMLKNLYVDLRVENIHFKDQLEWDMNNTMNSPEALAELTAKEMGMPEQYEPKIAHALRESISSLERQMEPKTGEDFSNLKKSTRTLIESNANYNQFKNVMFSNQINDKNWVRCELEYDHQFQWGPSIELLDQNALRNVQKQEDRKTRYVRRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQM5</accession>
    <name>A0A8S1MQM5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Catalase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0610230</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 H2O2 = O2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the catalase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQM5"/>
    <dbReference type="OMA" id="QVTWLFG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08156">
        <property type="entry name" value="catalase_clade_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.180.10:FF:000001">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018028">
        <property type="entry name" value="Catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040333">
        <property type="entry name" value="Catalase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024708">
        <property type="entry name" value="Catalase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024711">
        <property type="entry name" value="Catalase_clade1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002226">
        <property type="entry name" value="Catalase_haem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465:SF9">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00199">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038928">
        <property type="entry name" value="Catalase_clade1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01060">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00437">
        <property type="entry name" value="CATALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00438">
        <property type="entry name" value="CATALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catalase core" evidence="10">
        <location>
            <begin position="10"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8079266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="488" mass="55842" checksum="7301EE561C6C3560" modified="2022-10-12" version="1">MSDNSDNMLTQSTGCPVDDNQNSLTAGEYGPILLQDTHLIDKLAHFDRERIPERVVHAKGAGAYGYFEVTGDVTKYTKAKFLDTVGKRTPIFTRLSTVGGEKGSADSERDPRGFAVKFYTEEGNYDMTGNNTPVFFIRDPNKFPDFIHTQKRNPQTNLKDANMFWDFLSLVPESAHQVTILFSNRGTPDGFRHMNGYTSHTFRWVNKEGEAFWIKLHFKTDAGNKSLTGAEADELKRTNPDYATQDLFEHLAAGKTATWTVQVQVMPEQDGFKYKWNIFDVTKVWPHSDYPCLNFGKMVLNRNPENYFQEVEQSAFSPGHLVPGIEPSLDKMLQGRLFSYPDTHRHRLGTNYTQLPVNCPYRARVINQQRDGPQSFNNQGNRPNYEPNSFQPLKMIPESKTSQYRVTGLIGRYKPSHPNDDFAQPGVLYRKVMNDFERQWLITNIVGHLKNANRVIQERQVKVFTKCDPEYGERIAQGLGFPNNKARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTF5</accession>
    <name>A0A8S1MTF5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0700061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTF5"/>
    <dbReference type="OMA" id="FIPQEQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002908">
        <property type="entry name" value="Frataxin/CyaY"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01491">
        <property type="entry name" value="Frataxin_Cyay"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="130" mass="15761" checksum="04B5E409C20247C8" modified="2022-10-12" version="1">MFYLRNNLISLYRFTTQPKIAFISQEQFLGEIGYLLHKIEQSFNKLREYENRIEIKSDALALCVAVKDLGEYNFTTNVKERRLYLQTPFNLFQYYYDEQADQWKNVKDQHNMIENLTRETGKYLKGYLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZZ4</accession>
    <name>A0A8S1MZZ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0750132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZZ4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="26491" checksum="F84B2FD350F7C87A" modified="2022-10-12" version="1">MINCIFNQCIIFIYTKENVNQILLTQIDRQEQQVIYYFIRKQDLKSCNGVILVVNYFYLPQIELVIAEQIKQEINLEFMFISNRIQKHDYWVFLIFGSILNNNLVYSLRLPIFLFMSKLIFQFIVEYPKQRCKIILIQYTIKMKIINFCHYFIILLLKCFQLQFIRNKQNIQRIKLEQNLMIQIHLIKFFQEQILLQAYQNLLNHQIQIFQELWNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N195</accession>
    <name>A0A8S1N195_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0640198</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N195"/>
    <dbReference type="OMA" id="ITHDVVN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="238"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8080854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="390" mass="46140" checksum="1AB20A3AED9A6E95" modified="2022-10-12" version="1">MNNILCKHHPDNQIIFIIWNQNKLQLVCDECMGTTHQEGILKDDKWKKLAIRKAFKEPEYFLQQFKLDDKNQSLMKSIAKCPESQLRDLIQRIEANLKDIQITLDKVINDLKEEVCNILNNKSNFRKQLEKVSEYSKFKELIESLNNTSQTITHDVVNQVQTKLQEIFINLEQDTSNLNKEIQNFLASTKINRIYVFQGIQQFKEQIKQFLNIIEPNNKFIVSESYFKDFLLNKISLDAGKTIKSTNLILNEQQQNLNHYLFWKCVQNKSNLLMIFKSQSGNIFGAYSPLQWVYEDRKIDDRSKTTFLFSYTHKSTHKLINQDTQYSIYFEKENGPCFNEDLYIKRDFQDGYSKLGKAYSRENSKDPNTHLFGQKTPNIIECRIFEIIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2H1</accession>
    <name>A0A8S1N2H1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0750158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2H1"/>
    <dbReference type="OMA" id="LTHDYIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045606">
        <property type="entry name" value="ATXR3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46655">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ATXR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46655:SF1">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ATXR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19633">
        <property type="entry name" value="SDG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="264"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8085962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="825" mass="98287" checksum="9427484BF0E7ECA9" modified="2022-10-12" version="1">MIYSQQTQQEYKEMELQVFDTSDQSLAQLQQVKTRLNQERQEYMLFMQEYIVDCLIYGNQEIDDDVVQKIKNICQKMISKPLWNYEDLVVEFELQLEILTHDYIVLNQVKLIISSFDAYYQYKFNQISTQYLETLKSLCSTEIKQLKRMSNYIEVQELIPNQSDRVEEINELLTTIIQVQDNTLSMDFWNYSLYQFMSITQQDLQYQDKKQDVDVILIDTYGINQEVARILFQLLSDYEVILPQLLSAINITKGNLQHALSNLYDLQFKMIDQLNPFILKGKGIVCYQNEGFSGNEFICFYFGEVYTPQRWFEKQTIFHKRMQDGNRKTCSQSPYAEFFINDDLLFIDPTRYGNMAQHISYSCDPNCRLITVIVNQQNLLAVITSRKINYFEELTLPFPQTSLDQCLCGSIHCKRKQNLEVENSHQFSIYPNYIQRNVILLQSTLMSCQNIQNDIPEWLTNWQKLNYQQNNINILSCVDKVKFALQHLKTIQPPIFLVMNIFNQFWKNYEINTHKSQLDSSIMNEIVVFLKRHQQLHQCCIGLEIIKQMKQIIDQNNDYAMQLTRMLFLLLSEIILNIESCSFNSKAFATILYFMSFTHTYFSSTQYQGFDGNPFQENEFEYIPQPKNKSKLSLSKQYTPQFIWGQLINWNKQTLQNPQSSMAQERRGVLCYPSLLLSFDNKHKTFPYQCKTREKYLEYFQFKKEIQPDLSTWSYKNQHNIYGTIFFEQYFSQHRVGEDFINQICKLGMQKFEKKFQYWLHIENCFVMNQQIIDDLSTIFQEKFQTFFDQNPKSISSTDYDDNIKKKVKFNQDTIIAEETQFSTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N3H9</accession>
    <name>A0A8S1N3H9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0660078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N3H9"/>
    <dbReference type="OMA" id="FFEENYW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8081604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="167" mass="20091" checksum="6AB7951AAC087AF4" modified="2022-10-12" version="1">MCIEEVQIDKGLEKFVNVAMKYYSCRYGTKQDNWRSKAEAQWNGLPLYKDKMKVLEFMQSKIKQLEGPSIKITKFFKQIPHSDVQQQQEIQVAQIQEQSIIEEQARMSTVQKSKNSRYSKFFEENYWKMKLENEILTHEEIQLLIKKLWEENEEDRAHNMSKQKLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7F7</accession>
    <name>A0A8S1N7F7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0750145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7F7"/>
    <dbReference type="OMA" id="KCDKKWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="16937" checksum="BFBFD3FEAD60F9FB" modified="2022-10-12" version="1">MYYQCSKCRELVVDQQPCKCDKKWIILVFFVIVKISFIAFCYNLVIRNNFYGIFTFSIFILGLIALITSFFYCRSNQNDRIPLNMTLLQGQYNPLLVQQNPHVYINQFNIPQPNLQQVGYYQYQNQNIVSQNNQVQIPIKQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7I8</accession>
    <name>A0A8S1N7I8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0820044</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0820046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7I8"/>
    <dbReference type="OMA" id="NSLYMEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="12907" checksum="23519FE6C49ED6C3" modified="2022-10-12" version="1">MALQLQNKLQQVLMVFEDTFRNSLQQEMSQQDLQERATYMVQNQKELITLIDKMPDVDDSIQKNKLQEYFLEKDIQIDRHNQLKKELNELSEIVDTKIQEFFTQQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7U2</accession>
    <name>A0A8S1N7U2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0780084</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0780086</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7U2"/>
    <dbReference type="OMA" id="PRFGNCY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="184" mass="21465" checksum="8304E77CFF30D833" modified="2022-10-12" version="1">MQISDTEQECLNLFDPNTYQEGQGPRLGNCYQPPTYEELMAASTDNQLKLLPNNREKFFEFILKEQQVRLNPKVQQLYKDMNHSSAVEHAIHSELLKTFGYDDCPLNHKLYFALTRKYSNDPELKPQIFFWRNNVMHGSRVKLNETPPPIPLLTLDNKELQLEQLLQSAQEEKKLCVIFAGSYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NA39</accession>
    <name>A0A8S1NA39_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0700078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NA39"/>
    <dbReference type="OMA" id="ITRTDPW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18888">
        <property type="entry name" value="NUDIX_ADPRase_Nudt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF1">
        <property type="entry name" value="ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="42"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="198" mass="23127" checksum="20B8F2F6DCAEC2D6" modified="2022-10-12" version="1">MNQNTQATEQLVHAGKWLHFKLLLFNINDRQKIWEYTEKPPGYRGGVRVIPIVRYKNKPSKIIIVANFRPPIRKYCLEFPGGLIDKEGTPQENGIRELKEETGYIATLNNYQHKDVITRTDPWKSNEYCYNYIVDIDGDSEFNQQPQQSLDLEENILVYELSLGKSLAEELQQLQKDKDYEISGQVWLFALGQQFTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAG4</accession>
    <name>A0A8S1NAG4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Radial spoke head protein 9 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0700148</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell projection</location>
            <location evidence="8">Kinocilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the flagellar radial spoke RSP9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NAG4"/>
    <dbReference type="OMA" id="CLTAGEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001534">
        <property type="term" value="C:radial spoke"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006802">
        <property type="entry name" value="Radial_spoke"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055316">
        <property type="entry name" value="RSP9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22069">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22069:SF0">
        <property type="entry name" value="RADIAL SPOKE HEAD PROTEIN 9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04712">
        <property type="entry name" value="Radial_spoke"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8083614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="297" mass="34204" checksum="0E431ED067E61A3B" modified="2022-10-12" version="1">MEIYRIPEDLRFVNQIGQTLSAEEKIKLEIALIKLSQTQTFDQLLFWGRIEGTVANYYIAIGLNFKNNFEFPHKTFFYTANLKEFQNLPPLNPEYKEQVETFRQLFSGQPEKILINITGEDGDQPPADQPNPDEPQQVVKNDDDSDVEIKPPPKNFLEVDRLAYVVNAIEFECALLPVGAVRLTPTHELRYNDSFEGLSLQEASKISNYQHFRAPQSPNKKALIAQDDALFHYDFFDQLDDHPVGQWSLQTDSSKYQVTIRNLQWPGFLGYHRAGTRIFGYAYFGDGIKNVDLAFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBK5</accession>
    <name>A0A8S1NBK5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0760156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBK5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8086395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="158" mass="18730" checksum="F9456D82805EF1DB" modified="2022-10-12" version="1">MSRESQIELKRLGRSKAFQKTVEMCSCKSQNVLQNSQDEEVLMNESMHKIQQSNQKVARKSSIQLYLEKIFRNVECVNKKENNIKPLSIFNEEINKSKSKYHFRSNSQLYLTNYQSQLVPYYEQIRTTTNSSYYNNKKYSIKPLGMIKNKNKVIKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEL1</accession>
    <name>A0A8S1NEL1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0810002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEL1"/>
    <dbReference type="OMA" id="PPIRIND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="203"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="429" mass="51179" checksum="1CE39F088B067F14" modified="2022-10-12" version="1">MNQERKHHFQDKHIEILWRITQDNQIQNQQIQFEYVQAKYNEDLGFGIGVGGNVKMSNGQKYLAFPDEQGKVNILVDNQWVPLEKNLAEFQQYIPDQIQQQIPQYNQLNNEPSLYVEIRDQNNFKKYRGHITVSDYVSQTQSLGEELEDLYLEIEKNIQSIFDKNSSVFLNMMHKDPQLEFKLKMGKGKKLIDFFIPIKLTLVEQNQEEREKLQKEIKLYKQNKRLENIEQNVNQLQQNLTDHSQRIGFISQELGNQKTELYNKIQNVELNFQYQYQKFLQQKQNQFKKFSINQMKKVKEQDYIYIIQKLKGQCEFELLLVDDEIEFQVGVIEDDDLDNIEKKNKSYFVVLPDGKFTRGTREFGNFFTHQLQLEDKLGLRVDTFQRTFQLVINDYPFPPVKINKSIQEYSFIIKSSEKLNRFAEDTNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NF01</accession>
    <name>A0A8S1NF01_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0860162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NF01"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="61870" checksum="0B02C2A94D967592" modified="2022-10-12" version="1">MLYFLQSVDQFGVQQKLQIPLINPTQKSALGGLVTLTLYGISLGYFLFQFVDWQQNNKLPKITSLHQQINIDQTLKFQGVFAEISYFQELSNQIDPFDPQNMILQPQLIIQTSYYEETTINATFQYRKTQDGKTVNTLFIQDIEIKNSPIGSPDHKYINYQLSFGYCNPQLLQNGQKCAGQNLINQFFLQDNYYQILIYLEQFDPKSKQIVKVPREFIIEMIQKSLFQNQFMLKAGDLIVDDGFLFPNSNKYNYFSDLSIITTQFDQEHAKKVYGEELISIFYFNLDQIKIVNMVEYPKISEILADTGSIISWILSISFIVSKYNENLSIEKTQREIITMYYNDFIDFQIQKNWLGKIQRVNCKGKEYDLQKSQEILNRLDQIAIEKMNYLNLQNEVAKLQLILQEHIGLQQIKKYLESKFSLESLFDKLGIPEKISQQQEINQIHAQQELQQEVDQQLNIEGEIDSKFNKDIVEQEVEQRMGLLDMKTHENLQFREQNSNLQIQESLQQNNNYLTVINVDKTDEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGW9</accession>
    <name>A0A8S1NGW9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0890062</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FQIANHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1081"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1192"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1395"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1427"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="465"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="572"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8091790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1465" mass="173288" checksum="2CCF4860F0453BD5" modified="2022-10-12" version="1">MKAFETLRDKIISTLQNVNQKSATLSKPIKSLLIIQHQFLMLSYIFEQKNNSFFYIIATTTRPQLLLHSNNICYLILPILLLYMISIFVKKIPGRQKILVIGQLLRVSVYNVFFEISKGELQFIVQILLIIISTIDILFILGTLNIKQMNFSQISINYFQIANHILNNLILIFKQYSNYSHLIIIILCMLYSLNGFLNQIIYSPIKSTKLHPYILYAHLITIIQCILYFADIKLNEFPNLILCTLILKLIQISSANQLRFPIQNFDSLLLIIGDGCITSEHIKIIINKKLIQKEQDKILYATIKIKQKHYYEALCLLYNQKSSNWLNQIKKTIIIDECLKQLEFKIKTSSKFDQSVALAVEQLMKSEDRNFIIQEKIIEIIKQKQHIQKCITEQDLKIYQKLMNFVNKVQEVKRKLEKQYSQFPNQKTQAILSFLYAEILNDYISASKLSISASKQELIKFQNHQAIFNMFNSKMIYFITDFDQQRLIIKRMSSNTSQLLNLRHDQLINKDIDQLLPWGIREHHNEMIMNFLEEGISKYMRKVDLKYFKCHQSSYLITADFTLDISFTKSSLKFVTFLQPVIHQPMIIVLNQNKIITEFSENLIETLNEKPENILNKHMNQFLPSIDTIEFSQVLENVEMISQQSSYSTMKSYSIITTVNVKIKELKGRIIYYLLTFQFIKKMNQNYTYNQMVQSFSNGALSQDKTDIKTTEMKIKIEDDRFSGIFKEEITISKPYIMDEQNQRHQVNSTFFQFQQFSNNYLQINQDKQKRTIQQNQALQTKSQNNSTLISPNNSLYYDQLLSQQQYFCRNSQKQLSYVKEECSIDMVKELHAINESNQEEDCKRSQVSSVQNLKQSQHFKKYQILYNVNKQQCHSGSCLFLRTSLFSLIAHSIIIYIIIQSLYSSLNQLVRDIELLQVKNQAFQPLETFWVNRWIIFNYMKMKQNHILDEDEYNQLLIFPEYTIPLQFQRLQSNLNEIVNKKELQSSMSSINIKVLQYIDSDEGEIYEISIRSCINILLEFQYTLKQFYVENQLPQTDSAYIYYCYKNIFNLKQQFQILNDNIYEQSQINSIYILTQQRVLYLIQSILIIFNLIVLIILIRNVEKQHLKHLRLLFSCPKETYYQEIHNYQLLTKFILKDSDNLFKYQFFVHDKEKNLMKSTSTLMKSKDQIQIKKNNKDIKIQVGDQSIRIISFLIMFLLLIQGAYVYWMMSKYIISYQPTAQFYRKLSDVGTDIPTLYSHISVLYGISNFTFLNQNDKQKFINEIQDSLQRLENFVNIQNDQENILSTNFMNKYDYLQTANLCEYINQEIYDNPPQICNQSLNQNLQRGISSALIYILNSIKTERDLNQFNEKIGYDKYELEGALIFSEFIENLNGEFYQDLKNVTIQKLNQLIFTVIGIFCVLIFMMFFTVTVGKFYLTKKNNAISQSIFMIPFSVIFFDDYYELKLKKIQQKAFQSKQIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKM6</accession>
    <name>A0A8S1NKM6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0940066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKM6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8093807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="124" mass="15203" checksum="BE14C8A894BD7F66" modified="2022-10-12" version="1">MQQQQQQQPRQRTKERFVNEAINLVQQWRQVYQTETKIVDGRTVRITLDQAAELVGCPRKTLEDYYYLLRKAQTLVNLDEKRNEKMGYIRKLCRENKKYKQQIKQEEECYQINQFQFEDNIHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NL45</accession>
    <name>A0A8S1NL45_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0900008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NL45"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="74" mass="8964" checksum="E8E0198B08256220" modified="2022-10-12" version="1">MSFNKAIIRFYQFLKQLKGQTISVHEIKLIRSSLQEYVNKIIYQNKIKVFYSIIKMQMQNGILLSFFIIFEQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQC0</accession>
    <name>A0A8S1NQC0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0930171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQC0"/>
    <dbReference type="OMA" id="QVNKLGH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="429" mass="49047" checksum="CFAB8FBE3BD18E9D" modified="2022-10-12" version="1">MSNFILFGASDSTFFYVILTALKFIFSALIGFIIDHVQYTYNLLFGSQLLILFSYAFYLVFIVQLCSQQLIIDYLYFANAFLILGQQLGSIIILTLISKHYQRDQIPYQISYFQKYGFSGKLFCSVIVLILYIIINFQDKLTTETLLLINKQIIIILSIIPFIILLVISIFLHQTFVFFKLEAEELMPEIKFIPVQQKGNLLNAIKNIFKTDSAWIALSAGIIMGIIKIWLAYQENQVIKKHADEIFETFGIYMYFELPTIISLSLVSTIIKNFEIETISLQALIDKAIDSFVFFLLSILLSTSIVNDQIGKIIIFGIIRIFQQYYLTVISISGASIIGMQKENYKNQGLTFGLLGMIENGFAVWVVLAMQFGFKAENGIIYGFAFGYSLISIISSKMLQFDKQVNKLGHKVYSIEQKIDNVESQEEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQI2</accession>
    <name>A0A8S1NQI2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0990040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQI2"/>
    <dbReference type="OMA" id="QYETEWK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8095587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="120" mass="14769" checksum="68C37D080046E456" modified="2022-10-12" version="1">MSLNIYNEEEEQRFTSDYQMDCYERDYPQTMDENYTINYSPLQSKQSFKKAKTGQNKHIRKIKQLKKLKKISQYDKEWINFLIQRVSQIEEEGQFENLEIDLSQKEFQKILRRMKRLDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1A1</accession>
    <name>A0A8S1P1A1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1010095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1A1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="79" mass="9667" checksum="108C91D512A479DC" modified="2022-10-12" version="1">MFRRISNIDKLKQQMKLNQQVKELLQRGEIDMENPFHFEVILHENRLSLLYYLKMKNQGIKMIQMCLLQHSQLQNTFMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5J2</accession>
    <name>A0A8S1P5J2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1060077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5J2"/>
    <dbReference type="OMA" id="CPTKQDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="135" mass="15697" checksum="C0F075981467E0C4" modified="2022-10-12" version="1">MGTACSLHQQSIEQSPQLLLDHFDIRAYPFQCNAKLYTHCPTKQDISKFLIQQSKEFMDEELPSDSNGSFLEGSHRRTASQNTQRNSLDFSVTKTETYKKGILRRPQSQKTICQKSTQQKQQKKVHFQKSKFVHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5X8</accession>
    <name>A0A8S1P5X8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1060159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5X8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8098305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="158" mass="18638" checksum="19F60EF294B55EF0" modified="2022-10-12" version="1">MDNISTGKNIQNFGIFYTENIKGTFQLVAQIKLIIFKWFGSYDENCSGNKIGQWIELSDEFKWRQEVTNHGIYKNRNKIGKCHISYNKDDQDNICKFIIYLVMVDHTMKAVMAPRLKSGMNQTMNLGRDEKSFVMVNMKMVKKLVIRRCYFCSQIIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6Z4</accession>
    <name>A0A8S1P6Z4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2HC/C3H-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1070162</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6Z4"/>
    <dbReference type="OMA" id="YECTEGC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026319">
        <property type="entry name" value="ZC2HC1A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049899">
        <property type="entry name" value="Znf_C2HC_C3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555">
        <property type="entry name" value="C2H2 ZINC FINGER CGI-62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555:SF36">
        <property type="entry name" value="ZINC FINGER C2HC DOMAIN-CONTAINING PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13913">
        <property type="entry name" value="zf-C2HC_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52027">
        <property type="entry name" value="ZF_C2HC_C3H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="243"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="361"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="41"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8098681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="401" mass="46971" checksum="433BDABDE5FAD041" modified="2022-10-12" version="1">MSKQFKQNPQNSFNKLFNVQISVTSRPKYYDVSRISKPLQNLSNIPNRNQEDQIKKTQTKSPLKQNFQKNPLENKSSIQNLDEKSINKGISPLSRNIKTSEQQQQVQSDKQSTQQKQPIQFKNNEEDQINTNQQNILKSQQNIKQQDVLIQQKQQINLQKQSVQQKSQNNKQQLTRPKTASKSQLQLKQIIQQDQQINSTQQDQLLKLTHKTKSQMNLNQKQEQPIKNRQQQIFQRKQSVEDKLLQCPEGCGRSFNENALEKHSKICKKVFQQKRKVFNSQIQRMIEVENGEQDRGCQMKKQVQMQTNKLGQKQQQIIKNEKPKWQAQSEAFRAIIRQAKGQQLTQEEQVQLNSAMENVQNLVQCKFCNRKFNSEVAKKHIVFCETKAKQCGKSGMQKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P719</accession>
    <name>A0A8S1P719_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1080032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P719"/>
    <dbReference type="OMA" id="QKIYYEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="234" mass="28650" checksum="BBD61EB18CB9D204" modified="2022-10-12" version="1">MYQGTNKQIDQLKQEIEKTNIKIKKLEIKLQNEKKELKEKRMLFNGVKIQYQQYSKLEEKQTNNFYKIQEKLSKCKEEEYQKNIELQNDKYQISLLDYEVDSKAFQKAPFTQKQQFIEFREQDEIALNELDELEKEKKRIKNKQKQRIELTKKIEITAPKLQQTERQLRHNQYQKIYYEQMLDDVKYIIEQKQQYISNLEQSIHEYQLILQDLNSDIQTVMLNNLNFHNCSQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P733</accession>
    <name>A0A8S1P733_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1080042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P733"/>
    <dbReference type="OMA" id="QENRMIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8098850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="343" mass="40636" checksum="4DA853C284ACAD1F" modified="2022-10-12" version="1">MLFRQKEKIQDVGRNNFRDFYRMEHDAQTKTIKMMPSVQSGLSRSMNQPLIDENQSSLSGSSWDAKHLDTFLKQSKYSSMRQTQDLDNQAVFVGSGQNQSFFFRRQQPRIIPEYIDYLNMSKINHIVSDHPVNHSQEVIKKRLEIPEKNYVEQNLALSQVITLKKPSGYLAELKETQEQQKKFHQWREEFSEANKAYRKIRQAYKSGIIGIDNPTIENSELYKDEHQKYKQKQENRMIHSIHRYQSLEKYSSANPNIEFDNRKYDDSLTQINQTRKLGQYPVQTFEMDYKWMHKRTINPCQNTQQRLFGGVDKISRAASLRAENIKNQELRGRDYNCICLTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PD00</accession>
    <name>A0A8S1PD00_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1130030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PD00"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035863631" evidence="1">
        <location>
            <begin position="18"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="238" mass="28296" checksum="5DC55A899009A358" modified="2022-10-12" version="1">MVLISFIKILFLNPILLNQDCVELPGADCLNNGWTNAHNVISCQGIIYMGQFTGGRRISKTFWCPSENLMRLSFTIAKFDSWDGESVFVYKDNVLIGQITYAPFDGTKMCLGSYYPDLMEKKSYQFMLPKGQNFANFNQLIIYKQIMKVNEKYCYCRIMGNQRYNFISCRSMCQFLQRMQFLGRFVENMFRKSDFFIQICSFQNKINFYFGWYYSLDERPKLSRRYNLDIHFKLNLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PF40</accession>
    <name>A0A8S1PF40_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1150126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PF40"/>
    <dbReference type="OMA" id="VHAQQHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="130" mass="15211" checksum="494606D6EE7E0896" modified="2022-10-12" version="1">MGVCHSKKTQQNTYLQQDLTISNQQSLPPIIIRLDNNKILEKSPENDSQQSEVDLLATDELLNNPQQAAIDQQHRDQVLRHVHAQQHKQDNLKRYVVVFDKLYNQQRKVPILFSLEESTLFKRRKSGPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGQ4</accession>
    <name>A0A8S1PGQ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1180003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGQ4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="299" mass="34053" checksum="7816563EAAB74A78" modified="2022-10-12" version="1">MILAKISLHSLEEIIIFNWAIKLTDKYASTNNAATSKCRKQPNCVRFNAIDADQQQLLLPQLQHLKKKQVMKKLQQQLILNLILIQVDVKYSKYKTMHIIQRNQIIKQIIQKIIGLDINMNTTSEQSSDDAGNTETNKCKVRSCTETLQLQPILNVPLIKKCYINKSIGCVGAIQVMLVACSKFKGSLLNQIVLRRSAVIFQVNTKKNVMMVSHNLQQLMILSVSLMELHVFIMENFGTIFVQNGPRKTTKVGTAKETCVNRQFTETLNTLTTDTDFQKHHKDCCTSGYGYKSSMQNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIE0</accession>
    <name>A0A8S1PIE0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1190171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIE0"/>
    <dbReference type="OMA" id="CFAIKGQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04094">
        <property type="entry name" value="eSelB_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03696">
        <property type="entry name" value="SelB_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002712">
        <property type="entry name" value="GD25199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000052">
        <property type="entry name" value="Selenocysteine-specific elongation factor EF-Sec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000247">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049394">
        <property type="entry name" value="eEFSec_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049393">
        <property type="entry name" value="eEFSec_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF11">
        <property type="entry name" value="SELENOCYSTEINE-SPECIFIC ELONGATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21131">
        <property type="entry name" value="eEFSec_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21208">
        <property type="entry name" value="euk_SelB_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="1">
        <location>
            <begin position="20"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="514" mass="57753" checksum="477035EFBBF6EE78" modified="2022-10-12" version="1">MEQSLFISQQEIEQVNKQNTININVGVLGHIDSGKTSLVKTLSKITSTASLDKNPQSQERGITLDLGFSAFYTRNPNEEGKYFQFTLVDCPGHASLIKTIIGGAQIIDMMFLVIDVTKGIQTQTAECLVIGEILADKLIVVLNKIDLATDEVINKQKKIIANVLSKTKFGNQVPLVPVSAIQALGIEDLLKILLQNITIPNRQAQLNKGLLFMIDHCFQIKGQGTVTTGTIIQGSMKPNDEVFFPMLNLNKKIKSLQMFKKPVQMGEPGDRIAALFTNLDAKEIERGIVCQPGIVSLLENIVIDFKKISYYKGQLKSQNKFHITSGHLTVMGQLKLIIRPKNLQLEINSEGEYLEDYEDYVKFQEKYNLYGVLQLEKPLIASMNSLVIASKLDTDLEANICRIAFYGNILHSYTGQQIFQQNYLRIFRKKTKNGKVEKVIDNYTLLIKDLFKKETNIANYIGKLIVIKQGNIQGKIESAFGKSGKVKVFVESGTNQDMINSDVVMIYKKYLFHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJB6</accession>
    <name>A0A8S1PJB6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1190138</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJB6"/>
    <dbReference type="OMA" id="MRWKAFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23812">
        <property type="entry name" value="UBCc_ScPEX4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000217">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="3">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="1">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8102883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="167" mass="19552" checksum="6629CBBBD6FB4615" modified="2022-10-12" version="1">MICRPRLMKEQNDLQKLNDPTMIIQVNETDILIWYVYLFGPEETPFENGIFKIKVSIPPNYPVKAPTLHFLTRIFHPNVHMDTGEVCLEVLSQKWEPRWTIESIIRAVRLMLQEPNPDSPLNCDAANLLRANDIIGYKSIAQYYTSKYAIDKHEYMNKVNVKDKKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PS87</accession>
    <name>A0A8S1PS87_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1300037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PS87"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8106270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="121" mass="14130" checksum="8849F631C1C9439F" modified="2022-10-12" version="1">MNRSQSPINNSRNHSASKRYVSPNERQNTQENEQNHKKLPPTQQVKSKIINIDQQSDISSVLGEVNINNTSKMSNMTQFANFMYVPCPTHPEFFITNLCQDQSSQIRKYPKSQKRQYIQNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSK2</accession>
    <name>A0A8S1PSK2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1290109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSK2"/>
    <dbReference type="OMA" id="MEATNDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="171"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8106116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="574" mass="67605" checksum="29AF217CAB190CB1" modified="2022-10-12" version="1">MSTSPTGIQRYDNSPYVTKKQQPQYPIPQQYQQSTQQLNQPDYYDDRYHEFEEREEQYNYQIQKMNDHIQQLQHDLDVSEHAKQGFESDYLKVAHELQLYKDQLKKLYTIYQQQTVQFETIKKKTQESNLNVEKTYEKQIEIKNDVQNLILDRDLLLNKLELGDKQYNFDVQSLTQQLNDKNAEIQDQRKRINELTESKRKADEEIASLRFTLDHQTARLEAELREKVLKLEALDKQFAEALYLKDNELVDAFDKIRILEHEVRRILDLNRSYAQELSMLARDNRFKQEEITKVREDAKPAMPQQISVQSGGMEPRMPPAWAEKFHIKIDACLDLFERNHAKSLKQLCLNLWKTKMELKARNKQNKTMVEENSNLGKSVVLGGGSDMRVTTYFIDSFVAAITFARDAISNVVFDVFVKSVNKANNKKLNSFVLFFESLKEMQQKSSKGLWLALETYAKLRKALRHSVPILIDAKVQNGLLQKVELAMGKLLKHDMDFRNNINKWLEGTLAFTVQKKEAEAYDKNRDYLFLFRLLTVKYRIKFPLQGKTFEVDVDIETTLEHKICYALRKVVKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTF4</accession>
    <name>A0A8S1PTF4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T1310095</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTF4"/>
    <dbReference type="OMA" id="FSNDEPH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20335">
        <property type="entry name" value="BRcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20336">
        <property type="entry name" value="Rcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000050">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000051">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="444"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="175"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="179"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8106650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="475" mass="56409" checksum="80C8254A0B0FDFDF" modified="2022-10-12" version="1">MIKKQGKDYKLPLSKSDDILFVHGVDQEKQQNLRESQLRKSSLYDIALQIPNEQQNRRNSHNSITFNNENQKVKLLHTKKKILTQIAQYGFNAYMAEYIIQKQKISLEQDYQVVLDLVIEKIHQFEEQYKSCSGIQIQKASYKQVVPISIDEQQQIDQFIVEETRQELEKDSKYNNDYCGICLGEYKKKQKALNCRHEFCYECLQFYLDNKINNGQVLEIECPQQGCDNYFNDEAIKNLINDEQYQKFEKFKKQKLLDRDETIRWCVRPGCDKYIKGKSMFSNTIKCECGQEICYECRREDHPGMTCEQALDKYYELTLKQLVIQRCPKCKAPIQKKEGCNHMTCYQCRFQFCWLCRARYTRMHFDSDNCFGCPDKQFSNDEPHSRPRWKKYGGIIFELFATILILPIIPFILIYYSVMAPLKIVKHFNRQWYRELEDGDKLCLLLLGIFFFPAIMVLLICPGLLLLLAYKIKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PVP8</accession>
    <name>A0A8S1PVP8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1330028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PVP8"/>
    <dbReference type="OMA" id="IDYSTHN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000309">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF106">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="120"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="502" mass="58442" checksum="E821A185ED036F96" modified="2022-10-12" version="1">MQQIYNPDERMNIELFLAARELPNLEFLSKSDPYLELYYSLQGEPEISLGKTETAECNLDPNWEKSFIIEYLPNLIQILRFQIFDENRMGREFMGEAQITIKDILQSKNSLISLQIKRFDKNAGQLLCKAVLMKQSNHFVQWQFSGSNIKNMDGIFGKSDPFLKFYLYSDNVWCQIHQTEYIKDDLNPTWKEFEISLQRLCLNDENKKFKIECLDRHEKGKNNQLIGSFETTIDEIFNKNKEEFSLVQPKGGQVGTIRIKNKKRIYRANFDDYIKQGTTFNLIIGIDYSTHNGVPSFPDSLHSYIDNNNKYGKVISDVAQVLEKYNQKKLFAIYGIGAEPHLTNYTLNTYQTLFPLTGDFQKPQVEGYQEVANLYKNTLKQIVVKGDFQIEDYINYIQTIAEANAGKLIYTICVIIAQGKITDAQIFQDLLIKGSKLPYSLIFVGMGKNDFTEVKRIIDKINNQQPPIRNNFTFYEYKEQTIQMLSKHLLNDIPQHLVSYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXM2</accession>
    <name>A0A8S1PXM2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1350078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXM2"/>
    <dbReference type="OMA" id="RPFIYTD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="498" mass="58329" checksum="A01FEBFDDC9CAF09" modified="2022-10-12" version="1">MIKQEQDKNIWEWVPCNYDVKRIVKDTQTSWNLSPQISLKIPAENGTIDPLINIKIDQSRPFIYTDNLGWNWHKSWNLPDIGLEMEITDPLTNERLAPPNNIYCKLQIVKPIITPMKTHHLLEVGAKGNLKVDMENGKCIFSGLKFNTTSYNHDHQRFHIVITLYLCQNKFEFPQILDSRISPPIFVDSRKSARDIFKQKIQKLQSYFDPFLPNNLEKQFLIIKPQNSEVIKNSIDGLINYFTAPNIRHKVKHPIFLLLKFSACISLYVNSAKIKFTEPENLIQTLQHVLSTSNALQGIQKIEQKLIILYIDCKNNETKSQQNMKKIQEFLDPLNNDCVQVIMDINDVPKGFTQIQNLDDIQEAYHRIYNSLLKYKREDDQIDQEQLEEEYNAKIPEKKKKKELKESEQLVNPERIRKIIHISGSLPNQSSNSMPEQESNQIKMEQQFSISNNVQLRLPTQEQSQQYLQSQQLQQQQIQQNLAFNPMSLWLYLQKLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXY1</accession>
    <name>A0A8S1PXY1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1330141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXY1"/>
    <dbReference type="OMA" id="NDQNNCD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="226" mass="26574" checksum="98603FA27B959C3C" modified="2022-10-12" version="1">MEKSLLNIYQKQNMMNDNDITIKPFDDAQLEIESDNIEKKDNNKSSQSDVLMMDSQQNIKRQIAHIEQKDEINNQEQQLQFDSKKIIIQNVQQNEQNNFDNQSKLEQQDLNKIKVSIQEALGDDLQAYNDEIKNKLFSQDLSNHPQQTLKNSEIEICFNEQSLEQIVQSPEKNITTIPKKSKQNQHHTSTQVQNNQQQKAEKQLFFSKQQSSNNNQQRSSKYKYQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZK0</accession>
    <name>A0A8S1PZK0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1370133</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZK0"/>
    <dbReference type="OMA" id="THRSSKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="242"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8108586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="299" mass="34729" checksum="5C00B70F7C9E6FE2" modified="2022-10-12" version="1">MVFIYYHLGFAGFILLIQLYLLCRMYRKKNITNASAITPSNIMKTVPTSRTHHSTHRSSKHSAQSDDHHIITLQNTQRLHRTLQKKGTFTIESVPETNQQGRNSIKSNAAVSPARNILKTPTLIRKNSGYQIIKTNNRIETQQFHHHIPNNLPTIPSLQELPDSVRQSEIQFLGEIKQNQDNRQNYFYINTVYRVLIRCVISQGLIGCFLLLELHPALCVLIDLVAVWIFRFFQSNRKWIQILQLFISFGFDISILFLFQIDLTWTISFIVLAFIDSILEIIILKTQRSHSQQTIQTQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1Z9</accession>
    <name>A0A8S1Q1Z9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1380049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1Z9"/>
    <dbReference type="OMA" id="DYFIQRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035794486" evidence="1">
        <location>
            <begin position="17"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8108768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="692" mass="80534" checksum="FB463509019BA0B1" modified="2022-10-12" version="1">MTLFIVTSIIFMRVVCQQIILFASSFSDKKLVNLENWTLYPKNLLQTGFGSLSVDDDDYAGLYSMNGVQQNSYLAGMYKIYESIPAHSTLIIKAKTLMQNFGFGGTGTCRVRADGLLIIQIANQLDLINNYKINSFSVPYQSSSSAVLIEFHFEAIGDYQSLTFGFREFELYYKTCPPGCTFCQTSELQSNDCNFWSLDQRQLFDQSSLNEGWTISFKDKKQYRMDYCQDDKALNLIGFALPTESIERTLYLQPHYKMLILYKLLLLGADSIVFSYFYFELNDVIVSDIKFATYLKTSQICKHKNPSSMGDYVQQVKYEDFNQNTILKLRIFTQGRTYFQKLKWSIRNLEIFIKKCHPDCTESCFGPRANQCSRQKYPKFQNFINYFTEPLFTDTQEWQMITPIQPKSPNYCSGISIFGGYLQLGGDHYIQRIITLEDHQTIQISFKLYQIDNFNNDILYVVVDDQIVYQTVLEPIIDTSKATILCGIYEDYDLIVKISTPIIQHTQKKSIIQIYTNQPTTSQGYWGIRDFIIKQDSIIYIQELKQVRFLINDWINFAWIITQKVQTIEICKNKNYIISNNILGQPSKFRKIIKNLPLHSKIRIQFIIILTYTTINEDIIQINYSLDQNENQITKNLQLINDQYCETATLSKAYNFDDFINHSNTNLFLILSIDSISINWGIRDFQLSYSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2S6</accession>
    <name>A0A8S1Q2S6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1410037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2S6"/>
    <dbReference type="OMA" id="NIIEMPY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="164" mass="20040" checksum="8DB1083C4FB0A06A" modified="2022-10-12" version="1">MKNCQQNQRQNFYNFVFGFNKDQRSTSMKVERLRQITETSEIKQDYYKGLQLGKTSENALKVIHMKLERQRLRRQSFETKTEMEEEHLPQVQYPVKPPIPHRTIVSNKENIIEMPYNLAKQQQFQKLYQIKRRRLSQRMKIKKSEPTIDFSPWENEDNNEFDFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2U8</accession>
    <name>A0A8S1Q2U8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1420118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000146">
        <property type="protein sequence ID" value="CAD8110006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2U8"/>
    <dbReference type="OMA" id="FDANCWK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="152" mass="17846" checksum="B221E5CA4022B038" modified="2022-10-12" version="1">MYDQSQRQELIEFRNYIRQVTQAKINNTQDKSPSGKNSTKQLSFILKSTQLRQSVQEPIKNNQIMKTSYEQSYIQRSTSQPFVQFDANCWKENQNSFSKIKNEGYVSVSELIKINNGLSQMSKKEMRELNSNYLHSLHELDQTLSTLLKRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q882</accession>
    <name>A0A8S1Q882_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1490033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q882"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8111779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="212" mass="25182" checksum="756B54A860B9AA55" modified="2022-10-12" version="1">MVQNDSEATKQSSELQTGSKKITKQQGHWNQIEHNTYLKFLQENANHTKGQRLFKKMSLVVGTRTPSQCRQEPIIQDRITKNLIHKRPNLLLRIQDYYGDPNNMPEATLLYTNNLMNLVIDVNFVYILKYCQFYLKIVSQLIKIQIIYQIQINFPSFSCYILSFYDSLHIIFQKYYSNFNLPHLLSFKLTFLLIIQLQLLYVLIKFPTFIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QEV7</accession>
    <name>A0A8S1QEV7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1590100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000164">
        <property type="protein sequence ID" value="CAD8114408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QEV7"/>
    <dbReference type="OMA" id="VEVATQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="40489" checksum="AA02181A9A2FD3F5" modified="2022-10-12" version="1">MQNNNKQVRESWKMSLINKFGLTEVIEHNLIFQPPKPTYQIKNSLDFNGLEFSMQVNDNWIDLNQCYNLQYRVSILKEVENNVIVSYVPIIHYSCNSDRVIILSHSNAIDLAQSSRWGAKICELYQVDVICYDYSGYGITKKTMKPSEYGISRDLSNVLALAQHLYEYIFLWGYSIGSYPTVEVATQFQLSGIILQAPLASLGRIIDNRNSFYSEHDKFSNQSIINKVTAPILIFHGTKDTIIKINHSEQLSKSCQNLFAFIKVEGANHNDIGIAAETQDSEVYKYIKELLNNTNILPLNKVFELQLKFDNLIILDQIEESNIYKSNLPIQEKKEIKKHNDKQGCSLFCTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QGF0</accession>
    <name>A0A8S1QGF0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1590021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000164">
        <property type="protein sequence ID" value="CAD8114254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QGF0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36649">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36649:SF28">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="345" mass="40831" checksum="6BC0717AA936E3AE" modified="2022-10-12" version="1">MLYVLKEQLLRDLNIEVLKSDKIKSFGLDQVIRIFSQDQSCFKIRNEILDYDKIMEDKKLLEFHLVDFTQKLSNSLQIPIDQIEILGVSKGSFEINFLIKGKTYDEIQQLIQNIPAAQIYLHKYCNGKIEYLTYFDYARDTGEQLAKSSGIILSSDDFNPKYNMKWSNFKEKEQRGPPDHRYDYYFPRGCYGFGLDIKKYGENQDWIQMDGNPNEWRIMYHGTIQNFVNSIVKNNLMPGLNNLYANDDCQDEFGNMIKVGNGIYFSNDFNVCTRDKYASYTQVLDKQFAVIFMTRVNPKKIRQSQKMKLKNYFVVNESKDVRPYRILLHEKKKGIYNYLSFLFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJ93</accession>
    <name>A0A8S1QJ93_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1660018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000175">
        <property type="protein sequence ID" value="CAD8115742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJ93"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8115742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="50" mass="6122" checksum="C0F25B6853BA36B2" modified="2022-10-12" version="1">MNLILLYLIKTSLKKLKILEVIHTQTILQRTIFWDKQKQQTCKDSEEYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QMS5</accession>
    <name>A0A8S1QMS5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1940012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000203">
        <property type="protein sequence ID" value="CAD8117288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QMS5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="94"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="162" mass="19190" checksum="6BDD63D67E198270" modified="2022-10-12" version="1">MQSSSSQIIQYTMSKIEESFLELRSQIESSFDEIDELIDSIHLIPYKQVTKTIESKLELPQFPKFSISEFDQLQQIHESLKPLFDKIIKNRIELQEQKLNQTNFEQNIVDEEEESDQKKNIFKEEIDSENQQIKQSNNLLNETQKEVIQVENEQNQLELQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQK8</accession>
    <name>A0A8S1QQK8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1920007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000201">
        <property type="protein sequence ID" value="CAD8117224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQK8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="137" mass="16408" checksum="0BD34E744722BEBF" modified="2022-10-12" version="1">MRLQEGTIEIKIEWSFQLCPFTSGDDEEHIRLWDKKTRQQKLRLDGHTSSVRPVYYFSGNKLSSVNWDNSIQLRNVNLGQQIQPLHNSFQDKLAYLLRKLYLILFYQEIGFKFCFSHFLKNIQLWAKLWNICSQFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QST5</accession>
    <name>A0A8S1QST5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T2040017</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000213">
        <property type="protein sequence ID" value="CAD8117610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="474"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1136"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1277"/>
            <end position="1313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8117610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1419" mass="163220" checksum="6B8B35A6CF736AA5" modified="2022-10-12" version="1">MKNSQQFLMVVKWSAQLATVTMKITSVIKIIVIFSIFQGLLQIAISSNIQTQISLIEYNIIQLLQSAISIRILVTIKFITNQNIYIYFNERQILTIYINQFIYIDPNTKVALIFQGTDKIRIELNSKIDSTNLIIKFKNSEASSSIQIGDFQIEQELCVEFCQICKSYNQCQNCDTQKKLYNGLCVDKCPESTNEVQNSCFELDEKLDDQEHLFWKILVREFFDISTTKEQIDNLFTLENNLNNNLNFQKGQGIYFSYIPDKRIFGGPFVWVNAQFKFSLKLTEQEQVLKLSFEVFLGSKQNKQNSLHFTVNNNNNVQTLLLSNNQNVLEDHSWDDNYKTQKFVVTQMVYYQSSRQNELNILFHCDSDDNQAFCGIQNFKVISIQCQKGFLFDQYNYYLKQNPCIPICGDNLVVGNEECDDGNLEPFDGCYLCMFQCEENCSFCIYGECLFTLKSIIYKFDIIDLPPSQNQEVLCQLSCQICISNMCIQCSQGFYLNLITNQCETICGDSIVGGKEECDDGNLDNYDGCSYCQLVKFNKCDYDVDNSKQKCAVCHFGICIQCNQGYYKEDDNCYSYCGDGLVNEQTEECDNYNEIGCVNCKVADGYICVKLGLSPCLTXHHHMIMKHIFQEICYKTCKTCSNSEKNGCIDCYNGMISNSNICDSCNRLSNEKFLIIPDGCQMECPIGHSYDEDYICYQNNRLLQIAISSNIQTQISLIEYNIIQLLQSAISIRILVTIKFITNQNIYIYFNERQILTIYINQFIYIDPNTKVALIFQGTDKIRIELNSKIDSTNLIIKFKNSEASSSIQIGDFQIEQELCVEFCQICKSYNQCQNCDTQKKLYNGLCVDKCPESTNEVQNSCFELDEKLDDQEHLFWKILVREFFDISTTKEQIDNLFTLENNLNNNLNFQKGQGIYFSYIPDKRIFGGPFVWVNAQFKFSLKLTEQEQVLKLSFEVFLGSKQNKQNSLHFTVNNNNNVQTLLLSNNQNVLEDHSWDDNYKTQKFVVTQMVYYQSSRQNELNILFHCDSDDNQAFCGIQNFKVISIQCQKGFLFDQYNYYLKQNPCIPICGDNLVVGNEECDDGNLEPFDGCYLCMFQCEENCSFCIYGECLFTLKSIIYKFDIIDLPPSQNQEVLCQLSCQICISNMCIQCSQGFYLNLITNQCETICGDSIVGGKEECDDGNLDNYDGCSYCQLVKFNKCDYDVDNSKQKCAVCHFGICIQCNQGYYKEDDNCYSYCGDGLVNEQTEECDNYNEIGCVNCKVADGYICVKLGLSPCLTCGSNCIECQRINQDKLQCLSCLDGYYPFNDECLICDSYCITCKDQPNLCTSCYRNDCELCERFPGLYTDIQLKKCISKCGDGILIKESEACDDGNQNNNDGCNSECSIEYSKEMINSQKRWEYKEFGSQFKLSIGKYNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMP0</accession>
    <name>A0A8S1JMP0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M10 metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0050065</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMP0"/>
    <dbReference type="OMA" id="CLMYANI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012962">
        <property type="entry name" value="Pept_M54_archaemetzincn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15910">
        <property type="entry name" value="ARCHAEMETZINCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15910:SF1">
        <property type="entry name" value="ARCHAEMETZINCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M10 metallopeptidase" evidence="7">
        <location>
            <begin position="168"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8043414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="276" mass="32333" checksum="1658D5B9BA6517AF" modified="2022-10-12" version="1">MKNKQKLIKFEQKIQSQSLKIEIDNQIKNSEQLQTLKIISQNNEELMPQKNKLIKSELEPIGLLNLSLEKSINIQIIKSWIEIYLGLEVKILNLQLALYYEKNDFYIYDIDHNQTYKLEKDDKDIINVFSIFKVMTRVKPKNIQSFIVIVDQDIYDPYQPDANILGRAGNQKVCVVQVQTELNDFYSTIVHELLHTLGFGHCKDQDCLMYANISTSMQLCEKHLQKLSSKKEVEELKRYKALLHISKQIGFHNECQKLETLIKKVETQKDTLLQIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN21</accession>
    <name>A0A8S1JN21_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0040308</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JN21"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052788">
        <property type="entry name" value="RING-type_E3_ligase_ATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798:SF97">
        <property type="entry name" value="ALCOHOL-SENSITIVE RING FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="399"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8043085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="495" mass="57853" checksum="55C2B03CEF1AE357" modified="2022-10-12" version="1">MILFLFYFLVTTLGMIKLNINNEYTKKGQIYHLKEFEYYVIQEIENGIMFFQIESADTYKYLFIELIFETPKDGHVNLSFTITQNRQTSKYENNTILATYADLNGDYLHKSYQNILIPAYSYNQGDRFQINIQKRDEEITYKYKLKISKNNIIPCPNNCTSNTLGFCNFGICACEDDRIDLDCSKTGIPIIMDKQIENMFISGTQYFYFQQKTQLEKIQLDLEISNMFLSEHSQIFVYIMFENYIHGVATQQFNNQSFSFYSNPQSIYQSEIIDVSELNNNPDLLRFDRLLLTIVVPGTCQFNINISLPSEDSEKQTNQILIYVFVPLGVIILIVIIVCIIKKRRGVRPNQIGLDEENKKQGITLQQFNEYLKKIKLKDLDQHYPLSEKNLKSSSSQSCSICLQDYQKDSICLVTPCGHIFHDKCLQTWVVDKQNESCPSCRQVLNEQSLSKYAQEIKNQKLNQQLKKSDFQNKNSNFQIQLQAQMQTNSQMIQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNW8</accession>
    <name>A0A8S1JNW8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNW8"/>
    <dbReference type="OMA" id="NNQILHF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="142" mass="16750" checksum="2E01D20F483A3F02" modified="2022-10-12" version="1">MSKTEKKVLFEDDISSTQSSEGEDYIPKRIISKSNTRRVHKIPIELQQQLFRQVFQEGKQIKEVAKVLNLNYSSAKSLIHYYKNNKRSAPLEVLDVLSGKKQLVCRVSQKIDKKYNNLKIEVRSNNQILHFYNYYEHKTTNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPL7</accession>
    <name>A0A8S1JPL7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0060517</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPL7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="42" mass="5250" checksum="981C928BD941D6A1" modified="2022-10-12" version="1">MLTQKKFNRQWQKLKNKIARQEFDKNMNKQLAMRVAFVDYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWN5</accession>
    <name>A0A8S1JWN5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0110371</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWN5"/>
    <dbReference type="OMA" id="QQYGFIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="187"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8047324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="358" mass="42030" checksum="50680D830A77022D" modified="2022-10-12" version="1">MVLSVYSIVPVTKLTIEYNFNKGYLRVFKNSKSAKFLLLIEQLAIWKKILMLTEIYEYLEKNPFHKQEALKIYYFRHTVYQLRGLIFKLKAGLSPHIAISIFYKKISICLEGFALAYKQLSKKMLIVKAGFRFVRMNYKKNYYPKEIGKGSKELVYIVEEFQQYGFINYSGEVQYGELDIQITLKTCSVCMIAKVDLANNIFCRHRYCEHCLKIYFRNKYSIECPILGCNAKLIRRLITLKNQTQKARPSSAERKIKQHKIQDVEPNAATLLMDPNWPPKFIGSHVPNYLESQFIQLRKIIEYQCGERHFTQDDMINNNNQCSQCLMTLWDAQIKGNCNKHYICIQCTLNAQQTCLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXX3</accession>
    <name>A0A8S1JXX3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0090182</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OPA3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXX3"/>
    <dbReference type="OMA" id="YEMYTAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010754">
        <property type="entry name" value="OPA3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499:SF0">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07047">
        <property type="entry name" value="OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035895402" description="Transmembrane protein" evidence="4">
        <location>
            <begin position="17"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="107"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="182"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8045156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="25719" checksum="18FF645E175244D1" modified="2022-10-12" version="1">MLPLFKVFSLVVRVFARPVIARTKAAHLKKAQSGHTNWIKKFYVRLGNFQHKWDQKIDSKFMGIDKKSSDFFFKPLNDEVALEKGVEFFYEILIYALLITLPTYEMYSAQQDSKKKSEQNNEKLNNLMKQIEENKQHSQINIQKLEEQDQIRQDLVKQLNIISVQSFAQLDDLSKVLIQFLYQNWNLRSQQLIEENQKLKQELEQIKLKYSKDEDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JY04</accession>
    <name>A0A8S1JY04_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial</fullName>
            <ecNumber evidence="5">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="5">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0040301</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JY04"/>
    <dbReference type="OMA" id="MPGSIFR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000028">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="7">
        <location>
            <begin position="19"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="5">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="32"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8043078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="312" mass="33172" checksum="E9F75548075EB4B4" modified="2022-10-12" version="1">MIGKQIFRAFSSTTAPYVWVNKHTKVICQGITGNQGTFQTEQALNYFTKMVGGVSPKKAGSTHLGLPVFKNCQEAKSATQCDASVIYVPPPTAAAAIIEAIEAQIGLVVVITDGIPQHDMIKVKHALRSQSVTRVIGPNCPGIIKPNECKIGIMPGYIHQNGKIGIVSRSGTLTYEAVDQTTRAGLGQSTVVGIGGDPFNGTNFIDVLERFMVDPETEGIVMIGEIGGENEEMAADWIKKNNPKNKPVVGFIAGITAPPERRMGHAGAIVSKGKGSAPEKIRALEQAGIRVVKSPAQIGKTMLEVMKEKGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYY2</accession>
    <name>A0A8S1JYY2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0050049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="XDINILM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035889718" evidence="2">
        <location>
            <begin position="20"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="267" mass="30739" checksum="1852A9F5DD5B06AD" modified="2022-10-12" version="1">MKTIILALALIVLASSTQADVIATIKKIDQSPFGRTLFDTIWLELQTGDPLDRLLQTLTDLEDRYVAEQKEDDARNHEYQDACTVDISAFDKDLAESNRKKIELEARLEGQLYPQRGILQGLVAQKQAEVKGYQKDLDELDAQRAEENADFEEKVLEHQEATAIIAEARRLFADNIEHESFIQKGKATRQPVHKFTXDINILMNHKDSQLNILQICLVMMNFLSFLKKMMIIKVILRIPKKMLIIKIKLVNKQKKKSQICSFFQLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYY4</accession>
    <name>A0A8S1JYY4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JYY4"/>
    <dbReference type="OMA" id="LEFHTNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="98" mass="11500" checksum="7D5A3557B115F357" modified="2022-10-12" version="1">MINDLDINMCPLSPLEFHTNQQQIISEQFLEYENRSKKCRVSSQCLLIKKKIRQKKSRPSVLHIEEVVRNVKLYNTCSSQQNLENLEIIQNKPLVKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K112</accession>
    <name>A0A8S1K112_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K112"/>
    <dbReference type="OMA" id="KTMLNEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="15104" checksum="C9E6CC770B90AA66" modified="2022-10-12" version="1">MQNNDVFSPKNRNSDFSNVSVIQSSTQKTVLHEYQLSKIKIIVHQFEPKLQNLIKADGDQTDINPETERVSKRQNQRLDMFKNEIVKGQKIHKITFRDQFVGESLYQIKYFHKEPQEDLDECCPCNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1A8</accession>
    <name>A0A8S1K1A8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100111</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100113</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1A8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="129" mass="15894" checksum="F0B7FD6F5CAFCE0A" modified="2022-10-12" version="1">MRLTNLNTYLKFQAQFLILSYYREERNLRYYMHLKKESEIAKSVSQKADYKVKIAKKQKKYLRLLWPNKLLNQVYDQSRLELQQMLLLLKIKHSSEEQLQNYEQYFKRFVKSKYQSEMEMKSGQVFKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2I5</accession>
    <name>A0A8S1K2I5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">V-type proton ATPase subunit F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2I5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861:SF2">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="15803" checksum="E224780F726EBBC1" modified="2022-10-12" version="1">MSKKTFKKSEGTSLVSIIGHCYRIFINRHRRKEYQRRNQFFGCRFKYVNSLFLETDPKLIETTFQNFLKHPNIAVILVTQYVAENYLRHIINQYDSILPTILEIPSKEYPYEAKKDTIIQRAHRLLYGTDIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3B4</accession>
    <name>A0A8S1K3B4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0130274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3B4"/>
    <dbReference type="OMA" id="FTAQQNY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="125" mass="14656" checksum="78A1D5278D07723D" modified="2022-10-12" version="1">MKDKSTDKVTIFTAQQNQQHKQCYNLETSTSLKILRSLQTRTSIGNVCQENLSNLNKAIDRKVVEIYVKEKQNPGFLSSFLQLIYTRQQHPVQMEFEQHQNDSSEEWQPDDDDNDVDETDSFQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3X0</accession>
    <name>A0A8S1K3X0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0140300</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ERQNNTM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="668"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1046"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="192"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="763"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="988"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1088"/>
            <end position="1337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8050058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1492" mass="178487" checksum="4751D48B69F93048" modified="2022-10-12" version="1">MTDVEITPKHLKFQKIGQEKTITVKNNRRVVLNFAFRLFKNYEIAPTALQLLPGEATVVTVKLKNVEAQEDFIYIKGDNLDCRIAVSFECDSDRLNQYIDQDIQNRTITSPGFGNLETFGNVRSEQFNAEENKILQQRINYLTQQNQLLNDALEQFMNQEMDGNSIKDLLNHLIEQERQDNEFRSQRVLLMIEEKDREIDDLRLQLQKHQFNSNTKDQLKELVKENEKLQMKIQDYQAREQYFNQHQPERESYVMLYRKYESILDQNKKLESINRQMNEEIEVIAKRLNDSGLQNSNIRVEETRDELVNRLNARITQLKLREEKLLEENAHLEEMLIQQQGSSQNYSSNQSKNLTDDLLKKEYQILEKSKKELEKKCNDTISKSQILEKQVDEQTKQAGYLGDELWKSQRENEKLKQEIKKLKEQDELIKKERLEYESQIYALEQTLKQANSLQDVKEEARKRWEKELGKLQSSYENEIVAYQDDAQKLKKIINDLKKENEITNTQLKETQFKFDQKIQQLSQYEEQMQELQFKNQKLQKVNQQNQIDIEKTKSEFIILKEKTDIELQRRDNLEKQLQDRQLCLQDQINNIMKEKSITQSELQLEMNKLCQQHKASIDQLLMDNKSLEQQYLLVQDQLKIQRDQYAQREEALIREVRNMQEKIEKLVQNQKQKEKQRRNSRSNNSSVEKTVAPSLHNIEEERKISDLYMQKIRELESELNKTRQQKMQIEFDFNQLKTKSTFLQQGQTKDFLAQQQPILADDLNELKKTMIEMRLERDKIINQLQEKDYQIDHLSTQVQDYASLVQQQQGNLEQMEIKLSYINDDFKKTLEQKNSELHKLKLELNIREEQNQVYQEQTKLLTNERREINEKCYIQNFEIEQMKTNYQEVETSRKQLIQELRQKIDVLIKDNEENAEIIQKINTEIVNQKEIYEEIIERQNNTMELISEKFNQDMQELQCRQNQYFNQNDAYKKSLNPTKEQQWFKELIQSYHDNVVQLSQNMQKQKQNNDKSKQLQQTINQQKQQIKEISDKLNTIQTENQNLSKQLEKHKPKIPSLKKPSTSKQIQQEKQKYDEQVPVTLLLQQGKVAEVEIKSNQYKIQLKLAEQKIDQLKQANLIYEQELKKVNQSNSHLQDLLKNRDQTIQELQKQYKQENQKIINELNQVQNKFVPEDKVQALNEVIIQKDQIINQLKDEIQRKKDVADQFRGDREDQKQIVEKLKKQNEDLQTENEKLKRVNKDNERQSSNFKEFKEKYDQMKVQEKQIQDEIKSIQEKNKQLKQDNTRKETNIKELKDKIDQLQNQKPNDSEKQDLLQQVKKLKDEINRKDQAIKHFKQKFEEKSNEFEIYKTDYASKYAQTVSELEKEMRKNEQGKIGSKKYENQVNMLIFLLKRIYRENYMELNKGNQAKVCIKGENKPKNISKFSESMNILNLGPEDIEEFLEPNISVSTVKKSNNKDLDRFESLFMNPDQLDVNQIFNMINNVITERINCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6T3</accession>
    <name>A0A8S1K6T3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0170324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6T3"/>
    <dbReference type="OMA" id="CKNTHKS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="173" mass="20293" checksum="9360E68EDDCEF2C8" modified="2022-10-12" version="1">MNSLSQLQLLLANKPSNTKMFLTRQQIIDKVKKEKELQQIQSNRIQNKKISKPQSVMKVSIAKSSTQKLSQQKKTFSCSKFQINELVPDYELICKNTHKSLQNQQSTLNTDYFNFVNTKTKAIKDFQERKDEFQQIELNPSQMLSGLGQYQQMKNELMKSFLDIEQMLLRKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K787</accession>
    <name>A0A8S1K787_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0140103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K787"/>
    <dbReference type="OMA" id="HNFSHYD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8049665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="139" mass="16691" checksum="C70554B09AA5DA4C" modified="2022-10-12" version="1">MEINHLISNMYEQEIDTEEDYHNQKQWDRNMHFSLFGLHSPELSVIENCSNYGSQFDHNFSQYDNSGTKDSEIDICKSNKKIRKRKQKKSGLLSKNEFIKSFQRLEQCQSMQFFLDQLFHSVKTIKEHLQNKFVKVYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCF7</accession>
    <name>A0A8S1KCF7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="90" mass="11145" checksum="2304B2597ACD0D85" modified="2022-10-12" version="1">MHMLFNFSWICYYNYTYSYQSIQQIDEVFNXLKKIEQNEQRDPDMLTRLVKVITKPNSDNKEEVKTNFEYHEQRLKDMRTRTRQIQRAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCX3</accession>
    <name>A0A8S1KCX3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0200158</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCX3"/>
    <dbReference type="OMA" id="NDCAICM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="296"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8053099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="346" mass="41335" checksum="1F8B11959C582B0A" modified="2022-10-12" version="1">MNYQSQLSDSLIEQNQSQVQIPNFLFEQGRSILIKKNQIKMILSIIKYVFGMLLIISKFMLVTEEINLIQQHFRYSLQYIDIWLYITVIHSFISMVQYRVIQKYLNKYSIILFLAVKMGLVNIISIEQGLDQQQQQQQIEEQNDYQNQQLIGRLLLFDQRNIFVSKLSQEIYEKLQDKFKCLKILNKLLVIAFFFIQLLAVIILFNSDSLFLVDRLFYFLCMLYMILVVLFGLNQYFKTFVLVLLWIVFSPVLICYEIYKCCKRRKEQNLEKKKILKDLKEILYDQSKDHMENTDCAICMQILQNQEKVIVLSCSEKHVFHTDCIVDWLKLNLNCPLCRKQVIDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEW7</accession>
    <name>A0A8S1KEW7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Deoxynucleoside kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0200018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEW7"/>
    <dbReference type="OMA" id="FQMNADI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019136">
        <property type="term" value="F:deoxynucleoside kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01673">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003847">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002624">
        <property type="entry name" value="DCK/DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050566">
        <property type="entry name" value="Deoxyribonucleoside_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031314">
        <property type="entry name" value="DNK_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513:SF35">
        <property type="entry name" value="DEOXYADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513">
        <property type="entry name" value="DEOXYNUCLEOSIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01712">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000705">
        <property type="entry name" value="DNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Deoxynucleoside kinase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="232" mass="27633" checksum="4B23446FDE043216" modified="2022-10-12" version="1">MKSKQYTLISIEGNIGSGKSTLLRLMQQKYPDLRFIAEPVNEWQSINGDPSLNLLGSFYEEPSRWAYTMQVYAFYSRLKHWKEVLSDPLNPEDRHLILSERSIEADKEIFAVNGHKNGMINNLEYALYEKFYDWLCDEVFGKKIQKQMIIYLQVDPDVCYSRMQKRARDEEKNTISKEYLTQIHNRHEEWLLRETHQNTSILVLNGDKEFESDLIQQDKMFNTIDQFLQELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJA5</accession>
    <name>A0A8S1KJA5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJA5"/>
    <dbReference type="OMA" id="QHRYLAP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="386" mass="46567" checksum="CBB4AC138D37BC6E" modified="2022-10-12" version="1">MEQVFKTDFNFPGNIIDLTDSQQEHEEDSLQNKPLDGLTERILRKRKNGPLHQSLYEQLIQDPIYQSCVQNEFLFIKHLESLGFTDIYDYLKYCNKNGLLLEEEALKKNNLKPPQQKYNIYQCKIIHDKNTDFKNIQQILSGMKQLKKTMKLEQKCKKNLNIEFFTSNDTKSFAIHSTKQHRYLAPNLINKMLYEVEPLDQNIDETLIQYCWRQYRKFYKKCQAEYDAMVNENDNEFEGEFSETHQIDYDQISHDSLAPYFKVPPYKRKPQYRSMNLCYKIQHLKIVQFSKLCIFFFRDFIFLEGISFELFSSKKQQNTKIKVIIEVFIKFRISLKASLQSTCILQKVNFQQIKEHLTFYIIFKHYHFLLFCQQNYHLKKQDKFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KL97</accession>
    <name>A0A8S1KL97_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0240040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KL97"/>
    <dbReference type="OMA" id="HMEKEDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052920">
        <property type="entry name" value="DNA-binding_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358">
        <property type="entry name" value="ALPHA/BETA-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358:SF4">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="2">
        <location>
            <begin position="69"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="300"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8055938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="336" mass="38943" checksum="9ED2255285D23B4D" modified="2022-10-12" version="1">MKQQEYKWNEKLWKPIVRPPRHCYHLKDLGNELFKVKETIAKRTDFEIHNNRNQKLQCSLFEPLRMQGNPHPCMIYLHGNSSSRIESFTIIEYLIPANISVCGIDLSGSGLSEGEYISLGFHESNDVICLYDYLRENKSDITSIGLWGRSMGSVTAILAAHNNNDIKVLVCDSPFSNLTLLCQELAKVNYKIPNCCFNCFWCYVKSKIHQEVKFNIDELNIVQIIQVLPQDVQILFLSAQQDELIRESHPKLLMKQFRGKNKELFSFDGTHNSKRPTKIMEESVSFVLKAFGQNAWTELNTKQPTKNQQNQLSKQIPLLRGDRRSVQPQDERDDYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLG3</accession>
    <name>A0A8S1KLG3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0220181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLG3"/>
    <dbReference type="OMA" id="CDEKIYG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="285" mass="33981" checksum="4BF787DEF5439F09" modified="2022-10-12" version="1">MDNLINDHIQSQCPLLIFQEFSPRLKGPLYYKRLHKKSNEFQITNETPQKLIEKNWRIQMRPLMMPDLMGKSQIQTTTKKCINVLKYHKSQSCDEKIYGSYGSKWRRLISQGLFQVKQLRHKKILYRFKIFVRAVQFVIAFYKFKKTRTQQIKNSKRILKSRVSIVKPRKQSGQSTLISLPQFQLNDALQMLHQLHNKSKSEIHSPLSQLYRQKQKTLSQINFDNFLKRPIQQFIKNKNLFSSSEHKSAQASKRTCKTEYSQKRIASMQSFHRKLQMSQAYQIQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1C8</accession>
    <name>A0A8S1L1C8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0320107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1C8"/>
    <dbReference type="OMA" id="QSPRKFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="167" mass="20129" checksum="6ABAB3FE5A064110" modified="2022-10-12" version="1">MHNQGKRLYIDFQQQSPRKFQNLKNKTQETEIQFDHYGIVQTRFENKIKLNIKEHIDQLFIILKSIKEDLKISRDDMNIIKEDLQKMESNQYKEEDRITKTVLQNINKQKYELKKQQHTTSIVYGSLNQQISSLQNDKIFIKDHSNQVELDTKLIENDIGFRRIYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1S1</accession>
    <name>A0A8S1L1S1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome b-c1 complex subunit Rieske, mitochondrial</fullName>
            <ecNumber evidence="4">7.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0270326</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11484"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="7.1.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="4">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">The Rieske protein is a high potential 2Fe-2S protein.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1S1"/>
    <dbReference type="OMA" id="LQEFAIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03470">
        <property type="entry name" value="Rieske_cytochrome_bc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006317">
        <property type="entry name" value="Ubiquinol_cyt_c_Rdtase_Fe-S-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01416">
        <property type="entry name" value="Rieske_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="151"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8058646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="260" mass="29807" checksum="CF0CBF3659C8848E" modified="2022-10-12" version="1">MISGALRRSFIGKAHFGLVSEYNNRVNQKLYKGVQVSETPQFFTTSARPGNFGDHIDFKVQMDNWFDENRVHNEHETEIKRTQIYALNAVYYGGLLSFARLFAVGLIGRLNGWKRYDRDTYLEMDIGDLPPGEVMQIVWNGTPVFIRRLTQQEIKDEDNLPKETILDPNSEVVLTNCGNTKVLVVSALCTHLGCIPIPYLGAYNGWVCICHGSVYDKYARVRQGPALQNLPFINNSIYDDVIVCIEEMKFPREPSQRYWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L472</accession>
    <name>A0A8S1L472_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0330056</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L472"/>
    <dbReference type="OMA" id="NDDHCIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="110"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="274"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8062277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="643" mass="74808" checksum="A3406CA9CF7DAF21" modified="2022-10-12" version="1">MIMPSQFAVSDRQLFDFKKPQNNSDSEEEQQQQQLLLQNGNKETKRQKKFLDIEAIKQELNNRPQIDYENTPKVGRKPSRLMAKSLPNFIFPTSPVLIEQLQMVRAIRLMKEQVPPEVMNKVFSELRYLYVPANTAVYRQGDQNKKYYIILDGKCVVMKPREKMVGSKKLEQELEGKAQIQLMRRNDPDPYGLKSTFPDYIILKVLINGDAFGEAAIKLDIARSSTVFAIENSHLLFLSEQAYITLLDPFLSKSLDDKIQYFTQTPIFKEVEFELNEIMGILLECHLITYKASEILYEENERSNHLYFIINGQVELSKQLNSKHLVLSSYGENQHFGDVEVFHKIPRFTRARVISPRLIVYKIRQSKFFDNLGNIQIYENFKSTTKVILKHWTLIYQTAKSQVVQKDEIYQAAQDQLTNRRNDASKKIVNKKIIEVQGDKLSQVKVFQNLADSNFQDVNSIGNSPNKTILQRVYSNALQQYQARLLKKPQQTQCNDDHCIRSQKEKTFSTERASPARKNSQLNLKLPTMMSNTSSNSKVDLNQVFESISKLPRIPRDNLVLSLMYQQAYKSENPEKKAKQIQQVIQASFRNVRRDFESKEKVVSNWKYKSADSIIKNLKLSDASEEKRLKEDYMNFVQSKRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4C5</accession>
    <name>A0A8S1L4C5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4C5"/>
    <dbReference type="OMA" id="TEGKXVE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054559">
        <property type="entry name" value="PSMD12-CSN4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040896">
        <property type="entry name" value="RPN5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22241">
        <property type="entry name" value="PSMD12-CSN4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18098">
        <property type="entry name" value="RPN5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="1">
        <location>
            <begin position="318"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="423" mass="50278" checksum="046E84C3FBF4B676" modified="2022-10-12" version="1">MIIEENEFDKQIEDLLNKEKTARLANNLVETLKITKQIAKLCFDTKQYSKFNELIVQLSKKRGQPKKAQIELVQMAMIELKTLPINQKLEMIDAIMKVCEKKIYLEVEYARCVLMLTQYKEDDNQIADAAKILQEVQVETYGSMDKREKLEFILYQMKIMIKKLDYVRLFIISKKIEPKNIEDDNIADLKIIYYSFLVIYYRHENNYKETAHAYSKILESLHKNKQLEATKVDFNFRIDYNTILENYSMYTILSQYSEEKEKQLKSIVSTYKYSLEALPNIQQLIQAFLGTELISTLPQSHNIQAAEIFDENIENNKQRYIDFRRQLIHHNLRIFQIYYDSVYLNRITEQIGISTQELEEEICLMMDQKLLNCKIDRILGIVDYQLKKNENDVLQEWGDNVNKVLNLIDLTSNLIKREEELFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4D8</accession>
    <name>A0A8S1L4D8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0330086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4D8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004146">
        <property type="entry name" value="DC1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03107">
        <property type="entry name" value="C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DC1" evidence="2">
        <location>
            <begin position="64"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8062337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="106" mass="12468" checksum="5A56C157EC3E924F" modified="2022-10-12" version="1">MNQFILPYCPKYHQLQWKSKKIQSCLICLKEKKLSQYYCTECKQGVCNECIKPPLDGFYCGGNHKMQFMSNLPHHSCDLCEKSISQAYSCRTCDFDICENCRQFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L526</accession>
    <name>A0A8S1L526_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0310247</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L526"/>
    <dbReference type="OMA" id="DECICKR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000047">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF514">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="117"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8061315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="312" mass="35913" checksum="C143603AF77239E7" modified="2022-10-12" version="1">MDIDVDKIIEKLLESKGAKQGKAVNLTENEIRSLCVKSREIFLSQPMLLELEAPIKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGRQSLETICLLLAYKIKYPENFFLLRGNHECSQINRIYGFYDECKRRYNIKLWKTFTDSFNCLPVCAVIDEKIICMHGGLSPELSNLEQIHRIMRPIEVPDTGLLCDLLWSDPEKDIQGWQDNERGVSYIFGTDVIANFLKKNDMDLVCRAHQVVEEGYEFFAKRQLVTLFSAPNYCGEFDNSGALMSVDETLMCSFQILKSQDKKNVPLGRPRTPKYVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6L6</accession>
    <name>A0A8S1L6L6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0340144</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6L6"/>
    <dbReference type="OMA" id="WQQLYEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8063089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="273" mass="33775" checksum="CAD03C02566D6B44" modified="2022-10-12" version="1">MDAIMKLQKQNKKQNYELNLLWQQLYEMQFKFQQRNNPQKQSYEDRFSYTIFLSFLPFLYLHDILKLRLMNRHMKEIITNYLLYYRLEQKKAIKYLEGEIQEEKEKLPILYYEILEDLIQKSKDQAQEIIKLNYKGIKQIPTYTPLLVMQVAECEGIDLFKIIPEELIKMKNYLIFLDFSKYTDQKRNQLLEFYEMKLQQTNQIEQQMNIIDKSEVIFSRMAKMWLKQTYYQDTYQQYRLIMILIKKKDNLEQLVRQLKMIKKKKEKQREQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L707</accession>
    <name>A0A8S1L707_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0330153</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L707"/>
    <dbReference type="OMA" id="DHHTAQG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098796">
        <property type="term" value="C:membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000032">
        <property type="entry name" value="Export ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015854">
        <property type="entry name" value="ABC_transpr_LolD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24220:SF688">
        <property type="entry name" value="ABC TRANSPORTER H FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24220">
        <property type="entry name" value="IMPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="29"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8062471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="350" mass="39587" checksum="8C5209CDD6E24892" modified="2022-10-12" version="1">MNNDFIDKEIYEKEVIQIDPLIQQDKCIIEVRNVHKTYLLGLEGVTALRGVSFNVQEGEFITILGTSGCGKTTLLNLLGSIDLPTKGDVRICGQRIRASTEDKFLASLRLSSLGFVFQTFNLISSLTALENVELPMILKGTLSKDQIRENAQQLLDQVGLGGRLNHYPNQLSGGEQQRVTIARALANNPSILLLDEPTGDLDTKNSDLIMKIIIDLNVQKGITIIMVTHDTNLKNYSTRTIRMLDGKVANDEKNNEDNRIKHIDDLTRVVKQYQDINYGVRAGTVEENRQNTSKTEKRQPRNYKVLNHLYHPNQLLDNKQELPQLIQQYNTPIQKSNPNLLIQNKDSIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L881</accession>
    <name>A0A8S1L881_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0350099</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L881"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1161"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="45"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8063707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1269" mass="150440" checksum="CDE95F67DE33C9FE" modified="2022-10-12" version="1">MGAANCQSCCAKQESDTTELKLGRDKLKPKHDNSENDITQQEQKIQAKDHERFVVDQEQQKQKKETKKEPKKEAVKETPAKNVDENSKKSQEQDKQLNISANHSVSMNVANNQEGNDLIKSTMNCNERKKLPPIQLESGAVYEGEWKNGMRDGFGKQKWPDGSIYEGEWVEDKSSGRGKLTHADGDVYDGEWKNDKANGKGTYIHVNGAKYEGEWENDKQHGRGVENWPDGAKYEGQYFEGKKHGKGILNFADGSRYDGEFLQNDIHGEGTYIWPDKRVYKGQWKKNKMHGKGQIIWQDGRKYTGEYEEDKKHGKGVFEWADGRKYIGTWIQGNMGLEYTIYKIKKLKLENGMKEKEQNGLKKMKQINQLKNKKLKGKIYNNLIDQYNQYFILASQIFRINLFSNHICNYKQGYDSLQSTQYLIYIFMKVNQKKQVQFVKGTINSLENTSTIKYNHIEQNLINEKEFERNSNFTDSEFMSTCRELIVCQNCAKDLISQYKELYQTFLSRQEKFLFLQSDLKQYRIQCEIHTSNLIGSKRNDLFSNKKQKSSLNLLGQNRLSLMDFPKFLFKGEFQIIIRADVFKAQVNYFLKQMQVNETINNEKEIIMHINEIIDPQDNINFNEFIRQRDNQIIINYDYILFDKILKFGTTVLDIQIQDNFKSKILRAISSLILEQILNGRDQFIQKIVYLQVSFYKMTYMVKVLTFGLIKEFIKDNGRRIKCMEKDRQFGKMEGNILENMKKIKNMVKEFLNGLMEENILELGFKAGNMGLEYTIYKIKKLKLENGMKEKEQNGLKKMKQINQLKNKKLKGKIYNNLIDQYNQYFILASQIFRINLFSNHICNYKQGYDSLQSTQYLIYIFMKVNQKKQVQFVKGTINSLENTSTIKYNHIEQNLINEKEFERNSNFTDSEFMSTCRELIVCQNCAKDLISQYKELYQTFLSRQEKFLFLQSDLKQYRIQCEIHTSNLIGSKRNDLFSNKKQKSSLNLLGQNRLSLMDFPKFLFKGEFQIIIRADVFKAQVNYFLKQMQVNETINNEKEIIMHINEIIDPQDNINFNEFIRQRDNQIIINYDYILFDKILKFGTTVLDIQIQDNFKSKILRAISSLILEQILNGRDQFIQKYHLKINFIRILKQIIIQKIENRIQQIEYEKQQQMLIAYIEQEEKQIEVKKEKQRKKRQQRKLKKKVNQQLEQLEEEEEDDEETKNFFCDINFKTQKCESSHSDKEIITELLILKRNINEINQKRKQLRETIRKEWENYQKQFQSLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L915</accession>
    <name>A0A8S1L915_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0360237</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L915"/>
    <dbReference type="OMA" id="ETCAYVC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF32">
        <property type="entry name" value="AMP-DEPENDENT SYNTHETASE_LIGASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="60"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8064660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="682" mass="77483" checksum="FC72AD7661126A33" modified="2022-10-12" version="1">MEEKVYISEEGMRQLLKEFDSKDPLKNVLWTTNYRMELPIRVRKSGPGSEIPITLPEQFKNDCTQFAKKPALSVKRNNKWITRTYEEYYNESMQFAKALIAYGITEMSAVNIIGFNAPEWHISFMGSIHAHNLPVGIYTTNNPDACFYVSEHSECELVVADTKEQLRKYLKIWDKLPRLKGIVLYNDDIPTSEIPEQRRSQVFKWKDFIEMGKKADLMGSIIERVSKLRPGNCVTLIYTSGTTGNPKGVMLSHDNYVFTITQQKRKYVMQGDGEMRLVSYLPLSHVVAQLIDIIGFARWGAHLFFANPDALQGSLINTLKEVRPTLFFSVPRVWEKIYDQMQQIAKSNGAIKTKIANWAKSIGKEGTFAQTHGLKPPLCFGLADKLVYSNVKKALGLDQAQYMIFGAAPLSPIIREYFLTLNMYLINGYGMSECGGVQNMADPFHFDKFDELFINSTGKPMEGTELRIDNPDKDGNGEICYRGRHIFMGYFKDEESTRNTIDSNRWLHSGDVGKIDKNGNLMITGRIKELIITAGGENVAPVLIEDEVKKNIKIVSNCMVIGDKRKYLSILLTLKHQLTPDGQPTDKLNEDVIAVFKSLGSSATTIEEAKNDKAITNYIQQLMDETNTKVISKAQYIRKWTLIPGDFSVDGGELTPTLKLKRRVVEQKWLAEIEKMYQDAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDY1</accession>
    <name>A0A8S1LDY1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0350123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDY1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="31"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="125" mass="14706" checksum="23D47875F6FB6FDB" modified="2022-10-12" version="1">MIILIILKNYYKFSIFIIKYIFSIQYQIIAINVKQIKQKLKDLTIDSPLSWVKIIPDPNCRLLWNAIVAGPEGTSYQGGKFKLSISFPENYPFQPPNFQFQTKIFHPNIDHAGYMCYHTIERYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIL2</accession>
    <name>A0A8S1LIL2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome b-c1 complex subunit Rieske, mitochondrial</fullName>
            <ecNumber evidence="4">7.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0410029</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11484"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="7.1.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="4">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">The Rieske protein is a high potential 2Fe-2S protein.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIL2"/>
    <dbReference type="OMA" id="KRTWLIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03470">
        <property type="entry name" value="Rieske_cytochrome_bc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006317">
        <property type="entry name" value="Ubiquinol_cyt_c_Rdtase_Fe-S-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01416">
        <property type="entry name" value="Rieske_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="151"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8067520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="260" mass="29895" checksum="64F6CBD695BAE736" modified="2022-10-12" version="1">MISRVLRRSFIGKAHFGLVSEYNSRVNQKLYKGVQVSEAPQFFTTSARPGNFGDHIDFKVQMDNWFDENRVHNEHETEIKRTQIYALNAVYYGGLLSFARLFAVGIIGRMNGWKRYDRDTYLEMDIGDLPPGEVMQIVWNGTPVFIRRLTQQEIKDEDNLPKETILDPNSEVVLTNCGNTKVLVVSALCTHLGCIPIPYLGAYNGWVCICHGSVYDKYARVRQGPALQNLPFINNSIYDDVIVCIEEMKFPREPSQRYWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2024-11-27" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LMM7</accession>
    <name>A0A8S1LMM7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Vps52 coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0390191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007258">
        <property type="entry name" value="Vps52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048319">
        <property type="entry name" value="Vps52_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14190">
        <property type="entry name" value="SUPPRESSOR OF ACTIN MUTATIONS 2/VACUOLAR PROTEIN SORTING 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14190:SF7">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04129">
        <property type="entry name" value="Vps52_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps52 coiled-coil" evidence="1">
        <location>
            <begin position="61"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="146" mass="16929" checksum="AF64C47CCC514DBD" modified="2022-10-12" version="1" fragment="single">MSQQQQNLSSFLEHNLDPITSRYIMGKYLMMDGGEQSLDLRHMCDNVKLEMQLIEKEAAYDYLKVEKEMYELDNEIEKSDAILGELENVLLNFKDYLNDIKSEMTQLQERSVKMNTSLTNRKALSQILSSFVDQAVLDPSLIDNIX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LN68</accession>
    <name>A0A8S1LN68_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0390295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LN68"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8066783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="254" mass="30578" checksum="BBCBFC32F8FBFAD5" modified="2022-10-12" version="1">MKEKNELLRSENLNELYLEMNLVIGYLNSPEQIPLETQINQLTQGNDEKQSIDYINYEKGLKQQINIEEAILKQIIFEGDIAFFFNYVIYKIIKMSQQGIIKFTQELYYYQIFCISKNQNVHLKRIYDKLMSTYPDKFNRAIWSRYQISFEQMQILTITKNDIKHAEDFIWKFIRKKSKSQIIINLITKVEINKISFGFKFRSQYFLITLSISYLFKSIFRFKDINMYFNLFYEEILILIDTQLLELIGKGLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LNW8</accession>
    <name>A0A8S1LNW8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0440191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LNW8"/>
    <dbReference type="OMA" id="IGHDYCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051438">
        <property type="entry name" value="RNF_E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016:SF1">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016">
        <property type="entry name" value="ZINC FINGER, RING/FYVE/PHD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="44"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8069720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="192" mass="22505" checksum="B4C02E0C36FC3D18" modified="2022-10-12" version="1">MHNQRRNRKIIEDEELEDQFSKFQLLGNQNSSSQKKSSPNKQLCPICLEQLTSPLSQLQCNHLFCQICFDQFMKVTKKLQCPLCRQPFQYYDIYINQDGVLVWIKRSENFLEKNTQESSSQQDESIFQESFLNTPSQSIVSATFSEVCQVCLCNENDDQMIFCLLCRNLIGHDYCIQDDERILELCNSCQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTQ6</accession>
    <name>A0A8S1LTQ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0440172</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTQ6"/>
    <dbReference type="OMA" id="WTIYDLA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000020">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032451">
        <property type="entry name" value="SMARCC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16495">
        <property type="entry name" value="SWIRM-assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SWIRM" evidence="5">
        <location>
            <begin position="146"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="484"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8069702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="592" mass="69116" checksum="53291C088D301703" modified="2022-10-12" version="1">MNQKKMPQVKVTLKKVANGFIVHDADTTVRIPKSIVQQTPPQQTIQIKNKSQTTSLPSQPQIPQQQLQISPAILSPQQSFLYPQLNPGMQQSIQVQQSKQPIIYTSPQPPYKPIIQQNLINQPIIPQNYEIYPPDPFVTKKRSYDITVPSCTSWFRIDKIHSIEKENFKEYFNEENKHKTPQLYKKHRNFIINLYYNTPNIYLTTTACRRQLAADACTIVRIHGFLNHWGIINSQVDSDQYQGKIIPQPAIPDNLFNELFQSKNSSEQYQLSEQQIIDSISALSLKLRPICDSCQMKCNLVWYQQKPIKDIKEIILCIKCYGNNHFPNILCAEDFFKTDIEEKLKNINIQIDQTEQSDSQLNDQELSDMLNYIQENTDIGWDKITEYVNENRKVKLDVVQILINFLMYPLKKQSSIIRSLDGKEQLQKWTIYDLASRIASEEPQIFSDSSNLYAYHISIFQKRLNQNEESKQQNGDKLCNGTLHQDLLQQQTNLQNSLNDLDHNLIVHFKEDSIKKAENEIVKEENKLNQCINAIINVQMEKILQKIAFFEEYEKIVLNEKNILELQQKQTLAERLIVVQQKLQIYNEDNNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYQ2</accession>
    <name>A0A8S1LYQ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0490238</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYQ2"/>
    <dbReference type="OMA" id="HRFCMIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000027">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054694">
        <property type="entry name" value="Parkin-like_IBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22605">
        <property type="entry name" value="IBR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="103"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8072657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="480" mass="56905" checksum="79F902CEAB419E08" modified="2022-10-12" version="1">MQLDYPNEFDCDNDSVELEKQFSLQGVEILEIRDVIQVIIKEVQELSEELYFNEDNTFELLMHYNWNREDVIQNYFINQEALLAELKAKGIFNNHEKIIYNGIKGCCQVCLFEGNLIQLGCSHKFCQSCIEQTIKQRVYEDKYLVARCLQDGCNYRLPFSMLKKYSNAQEFENLLCRRFVDCSRYLAYCTGVDCNKILKPQYTSVKEVTCVCQNKFCFYCKEDLHPPCPCDLVKKWLAEIKKDEANVRWIVVNTKSCPFCKKPVERSEGCNYMMCKPPGGCGKAFCYVCSQPWEPDHKDHFKCNKYVAPTANIEKEKEVLQRYNFYYERFLNSQAAKEKAMQRLKQIKEQYITSIFKHYQFTYQDSQFLEEAMKELIQSRVVLKWSYCIGYYISKTNQQSAKLFDHYQDLFEHACETLAISLIKLFDEIEKLEYTQSEKPIHEQKKDFVEKKERIQNASQKCCKMRQNLETAIYQGEIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M028</accession>
    <name>A0A8S1M028_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NLE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0500094</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M028"/>
    <dbReference type="OMA" id="VLCAEWE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002225">
        <property type="entry name" value="Notchless-like protein isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012972">
        <property type="entry name" value="NLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF0">
        <property type="entry name" value="NOTCHLESS PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08154">
        <property type="entry name" value="NLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="6">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="116"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="158"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="201"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="248"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="361"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="403"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="445"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8072879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="479" mass="54100" checksum="66A672E6E2EEBD48" modified="2022-10-12" version="1">MDQTKKKIKPNQITLLPPDTRVFVTLINPYGEEAQKSLELSVNTTKEELQSILQQLVKTEEDQVYSFFHNNIELIDTLNQLIASDPEYKLENTFSLTYHPQSLFRIQPITRQTAALEGHEQPVLCVQFRTHGDVLATGSGDTTIRLWDMLTETPIATLKGHKNWVLCLAWSPDCKYIASGSHDGQVCIWDVETNSLKGQPLIGHTKWVTSIAWQPMHLDEECTLVASSSKDGSVRIWSRISLSCLISINAHQKAITKMLWGGQGHIYTASEDTTIGVWNKSGKRVQELKGHGHWVNSIALHTDYTIRCACFSEGNTEINRKQAKVLFDKMLNGKNERLVSGSDDQTLMLWEYTSSKPKVRMTGHQQQVNHVQFSPDGRYIVSASFDKSLRIWDGYNGNWIATLRGHVGSVYQVSWSSDSRYMLSASKDSTLKLWSLQKKKLAFDLPGHADEVYAVDWAPIGGEKAGSGGKDRRVKIWRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M2X8</accession>
    <name>A0A8S1M2X8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0520173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M2X8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="326" mass="39221" checksum="BA589AD8E4B15626" modified="2022-10-12" version="1">MTKFLFIIVKPNTSNIHCNDNKKQQSYIKRQIQLCWLRGRVYNNFLTMNLIEIPMILKEIHNEYLLLDNKVQHLDDKFELIDFGLKLITQPYCIPKWGDENVVIYQSGRRYIYEQKFENDYPYHILCVDRFKNKYGVLNKLHKAISLHVFELDQIYEWKQTNTINDNDIFIELRLIINEKMPTENLKEALLRIKFLDDKQIYITSKKGEIFLISLTKQVKQLKLDANVISDKYFYKLPQEIHIKGIYLINNYQYKYYTVGLDRIIFSFCIEDSQIFDYHKFACLAGKVNKLQINKEKNLKYYHDKKLKSLQNINMIKCVLLLKIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3A4</accession>
    <name>A0A8S1M3A4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0490051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3A4"/>
    <dbReference type="OMA" id="IPQQLKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="179" mass="21262" checksum="BE543E0C70A9EE24" modified="2022-10-12" version="1">MQQRLQTHSITEEGRKSSTEIRSPLHEMYQIRRNVMKLEKQRTDESCHRKSEEFIKNISQSHRDVIIPQHLNFEDNLDGSRATINSINSVAQHYEMIKPNVHNIDWNKENNQNLSNKNKHHQKCDSNKHKTKVIQLDNQRQQINRTIQQMSKCNRQLANVLKAQIDKLFDTIKTDYTYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3Q8</accession>
    <name>A0A8S1M3Q8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0520175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3Q8"/>
    <dbReference type="OMA" id="LYQCDKL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="43"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="186" mass="22209" checksum="59E633EF4A4A0BC4" modified="2022-10-12" version="1">MQNYYQLPLSPRQFISHNNCTLNQSPRHYAQVPISIVPQQAQVVDLKQYEQKWMKKIQQLEEELCYCQQKASYLEKEYEDLASEAQGYKEEIKKLKSNLYQCDKLDIMNQMSRTQENNTISTALDSERRLDDKEDNLKDQIIELQKVIIKLQRESSQNKIEAIKWKNKYIEVNKLYNELSVEKQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4M1</accession>
    <name>A0A8S1M4M1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4M1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="323" mass="37878" checksum="81639BBD975CED31" modified="2022-10-12" version="1">MGNQCNHAEINDNEFKSVEIEDESDSFQVYKFDPKIHFLNTSDLGKFQSLESLNSTKSQFHDRVKKVPFNLKLHRKRTLHYESIHDSQVQSPTTQVSDYFEYPNGVYQGQLIDGKREGQGQFFYHDGSYFKGTWKNDMANGLGKIKFQEGEQYEGEFKNDQQHGFGKYISNQLKYEGQWKENLPSGHGIEIYNKYYIYEGEFLNGMKHGKGKIQWYKDDSQYEGEFSDNQINGQGTYYFGDGEIYEGEFKDGNMHGYGRFLWPNGKKYEGNYHMGRKQGFGKFTSKDKVYTGEWYDGMYHGYGKLQNKDKVIEGYWEKGVFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5I6</accession>
    <name>A0A8S1M5I6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0450275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5I6"/>
    <dbReference type="OMA" id="VQFFKRE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="627"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="260"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8070474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="991" mass="118034" checksum="39E559B7FEC6B2D7" modified="2022-10-12" version="1">MNCYKKEITKNWLYQEDKYIENIVGAFEVIKLHEERSVQAGNARQKITEQLQKYIDEDPEDLPQHLSTQHVLSLDEQLLDYPCYKKGGIFGSVTQRQCQLQKTTLGQSKKGNVIIKTFGVIKTFYIQHLEDANKDFKWDVSYQKFPVRIMFNFVKHSPVWLYFKDDEEAQKYYDYFKCHIAMNKIQKLPIIIQESTSLVKENYFLLFAQKLETYSIHVIQQRKKAAKLGKDEAKFLMEIDVKIIRNILQKKWLSKSVQFFKREERMNQLAEEERKKLDQIRQEDENKKMKWRQFLGSKSVLNVELAEWLRPMMADWLEKSEEIQNTQKVRELVFTILIKSINIAVDINLIKPTLFVELQSVEQDSKLTMEAYLGQQKTDHFYNWLLREQNFENKIISKQQERKPCSFIISNINNQDQFQIQFKDMVNAFYDTATIPFKIIVKELLQQSQQGRSMWIPLKLVQTNSLKTESKESIIYVEIDIHVKPSIMMHNPQSYEEELEYIVEHSLAFSDPIYLSTIQIPKMNKDIMKFILITLEESNICMKQSMQMLSYILFLQQKNPETLNKQMHQLQMVFIRKVILNLCHNFGLMEKQIKQYELVSYRLESMNFYNIKNPQVQELVNGLFLMGIPYYLRNKLWFQLLQISFDPIKQVLSQNYKVNLDREDGMNESVSLYFKLSQLKQNLKKTLLQQIDIYLRQEDSRKGFGNEQFYHQLKNLLICFLLYQGNQGLIPNQIYIHHLFTFAKKMLVMQIYYNVRYNDYTEFANETKQTPSDLINESRAFWLLVGFHNQVLADYPLYEDQTLQNRLFLEQCAKLRVYLNKKHPDLFHKMFLYGIEVENELFELFFNLFSDVLPSETLYRAWDLIIYQQQQSIRATSERGNIIISITAALLLRVHKKINNISNHLAFLAAIKVEAMLINDVTEFLTEVLQVQKDLNLAVQIEYQEEGPPKLEPQVEYKIKSDYRIFDVLDEKHPKGSQQMQVYQYLNFIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M7X3</accession>
    <name>A0A8S1M7X3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0530217</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M7X3"/>
    <dbReference type="OMA" id="HILYDQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="173"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="573" mass="68682" checksum="8238955DE194BB1F" modified="2022-10-12" version="1">MQRPLTSITSSQQQSKLYDNSSTSFRIKENKKNRQMPQQMLYDKEGLYEENLKLKKELQQVKFELKKERQEHANFEKQVQKTNETQKDFQVILSQRARIKEQDKLIKELEDQVFQLKRNPERTKNKELEAELEEQKEAYQKLQLYISSKCNQDANETVMINQSLKDQIQINLVQAYKQDNLNLSQMILSLQQENHGLQQNLLQIKNDKDRLTNRLKELEKQFDSVNKHMMYMRQEMQNTKKQTPSEDYLKKIDELQQQVQNQAKQLQQNKKLIEELEQKNGELQHNYDVFKSMENKEKEKLQDRINMLKKQIEELNQKRDDQEQQSQEGSLQSIGLKQAMTIYGSPRSNNSPQKLQLQMGFTNKKQCLKVNSYDIKEIISNLKIKLLSNNIDYETQEQILCEDDDEFLTLEETQFRLQKAPFELNEDESSLLARYLIEDNTEDHILYDKQRTQTKIIIKSVYKKLLGEFNLFDEIEKQQMYEYFCQLFHKYNQGMESAIKQFNIKKKYLGNNQCEYEDFNEALKYCDILLTSRQRLYLELEIFSDSQQLQRFNFQTLFSKFKLKVPDLDNSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9Y5</accession>
    <name>A0A8S1M9Y5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0510013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9Y5"/>
    <dbReference type="OMA" id="QYGNSSH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029147">
        <property type="entry name" value="CFAP77"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14825">
        <property type="entry name" value="CFAP77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="162" mass="18956" checksum="60919F84F01D6E85" modified="2022-10-12" version="1">MSTILPYLINKTNNILLLRGDVGKAKPSVSDLPDSSYYYGKKINRDGIGMKEITSDWQYGNSSHHKKSKTVKLPEDTFVYGLRNKPSTPFGKVIKMNYGNEAADIIENLYRMRSQRIPKKQVIQKNRSYSLRLEHNTKKKEEEHKEFKIKRFLEIKSKIYQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAG8</accession>
    <name>A0A8S1MAG8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PPRIM_AZ9-3.1.T0540029</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAG8"/>
    <dbReference type="OMA" id="IMYSIFN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="12"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="441"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="507"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="446"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="132"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="17">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAD8075091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="670" mass="78162" checksum="1999DFB7181E57B6" modified="2022-10-12" version="1">MNDQMDIEYDLFEFQGLLGQGSFGTVLKAFNKNTKQTVAVKVIKKKQMLQYAQLMQEAQIQQELQHPNIVKFFGVYETENKILIEMELICGGSLSSITKCTEEQAKYIMYSIFNALQYMHNNNIAHRDLKPENILVTHDLSFVKVTDFGLSTGYSSLMTKQCGTLIYMAPEQLNNRIYNKAVDIWAAGVIMYQLLVGEHPYYKKGCDVAQSQLTMHEKCKKILNDKQFSLFRRLTEINPTKRYSATQAMLHPWFNNGSDEPLTMEEQFQQWKQSQKFQNIILLLMLLSKIGYKKVLHNKELYYQISKNLNNKKKQDVMNNYDQLFAEYQKSLTDAQKTKISTVVLTPRIEQNNENSGPSIFQSSLIKPFKLQSQFDKNDATRIINTNSQVQTPLNVYDNDLDQQIQFSKQSCIIKDRLDKQNIRPQAMIQSQPTKLTSNLSEIDSNTQDNDTQQQQLEKQCQQSTATNTTSASPTKSPLKRKRVKRTQIKQYLNEEIVQSNLQQQISNNTQKQLQQQQQQQQQQQQQQQQQQLQQQQQQQQQQQRQQQQQQQQQQSTTNVIRQQSPSPKNKFKIQLKPLNHQQQPINYNSQQNGNQSLRLKAQKLSVDREFLAPPVLDLSNLALECLSPKYRKTRTNSLHYQKRVYANEFQPSRILGGAYKKNYDSFYMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDA2</accession>
    <name>A0A8S1MDA2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0560007</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDA2"/>
    <dbReference type="OMA" id="ESHCIRV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF159">
        <property type="entry name" value="LD03170P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="84"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="428"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="4">
        <location>
            <begin position="631"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="393"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8076241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="975" mass="115532" checksum="82406E70BDFB686D" modified="2022-10-12" version="1">MNQNSNQLLGQPKNQFKIVESVYQQQQQQQQPRQQLSVQQKPIEESHCIRVMSKEIKAVHRMQLWFYGKKQQKLYQKMKKKHRYRKNVIAEIINTERTYVNDLTVICEKVKTSSLSIISPNDVETIFMNCDQILAWNREFLLEMEKGYQKYNLDKKFIPKPYQPVFDGNAKQLKTPDINAFKCYYEYCSKFTKSNKHFEHLKANDQKFKEFLIQLNKNNTLRGMDLGSFLVKPIQRLPKYILLFKDLEKNTDEDYPDLPNIKEIHKYFKEVNEENNKFMDIFMGRLQLQQLSESLKINQEELLNEPNRVFKFEEKLTIIQPNAKSDEDCQVTVYALSDMLVVVHDEKVQKKLKLDSLSFVKNQSDNNKYFSNLFQIVGVGDTIQCIAETPVAKKKYIDQFEKLIQENREHEHEKEKELCKQQIKMRYPVQVVVVGTEERNFQSFTKHTQYITEISINKVFQNIFIRYSEIEAMSSKYKQRYPKIDLPQLPDKNWLMSHKTKSIEARKIAIENFLQALLQAKESQQDPDILNELKLPKNFFDLPDLYQSILKSNLTQKQQNFKRTTLEGKEELREKGINVEKLKISAGEILKAVYIEQETRKKSVVQICPNKVISYENSKDPNLQKVIITFPDGTETALGVTEQTRVREVLEFVAKYNHLIYYKDFRLYLIDQLKKQRVLDDDEVLYKVIEDERQNNHGILNKLEQMLAKSNSEVLIKLKKYIYLPAKLEESDYKEDPVRLINLAFSVLDDVHDQKLQLGFKEYCLFAALYFHMKHQRLDDKLFGEVRRVIPQEMFQSSTDKEWKDLVTQCFKALDNELSQISKKNENDQIQKKPNSQYKHTDKKYLAAGVTLNAARTFVQSSMAIFQVQVYEGTQKFFKNSGLEVHPTIYLGLSIHKIHFLNPQRKEQFREIDYGRVNGVKSYPSQIIFDLQIFKEPIRFDTYQSYEIKSLVEQYQAIQRFDEEYELGHQFNIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDI4</accession>
    <name>A0A8S1MDI4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0510017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDI4"/>
    <dbReference type="OMA" id="KETTWGQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="415" mass="48956" checksum="10BAF1FDDC82C6EB" modified="2022-10-12" version="1">MIKPKKSSRELWNQLGLRNHIKQAFEEKETTWGQIVDSNKKNLSQRSMSEYTIAQKLKEQHNSFIKNKEKPNTKTFLEDQIEKGIQDKDEWLQLASINEKSHLSPITKRNSNDQTFSPINRLKNEEQQVSQQQSFQVQIEKTNICKLPGHENNQYKFICIDNECPYKLQKACAHCVIKMHTKHVSQMKEIENYEHLIEQNIKKADDLMDQSQELFKAIQKPYEIYFHQIKNQIITLINDLQQKLLQINKDQIQKMINNDIEIVQNYNSKFEAYKLMNQLIPNEIIEEYTHIINGISEKIKLNINFDNINKQVESFIMSIFCPKKQKENKEQNDDTELLQQFIQTNRDSNKESILKPNHSMTCCSHQSPLQTKKQLQTKLVMGSASPLNVFNRQINGRQVTKIIQTQPIIYYRTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDZ4</accession>
    <name>A0A8S1MDZ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0550059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDZ4"/>
    <dbReference type="OMA" id="APRECHF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="387" mass="45325" checksum="00C90A0F56B8A27A" modified="2022-10-12" version="1">MSLLSKFTSIFGSNYNTQTSDTPLNQQLQEVLSQILKTNTKIDLKQKAELLAQFLLEKEEHIDYLFSFLQTTSKDLNSLSVVHLLLVIHQQFQYNELIQDVAIKLRETKMPWIQLEKHEWQSTKTEPDLPKKQQSQIILTEFEDSSRPAAIYAQLCYVYLQRLAANIDLYRAVSKFMYPYVSDKDQIEPKLMFLWHYKIQNLINSGLILLQYDQDLVEIQTALYQDVWKFQEFICTEIEKIIDQYVTLPSSDTLSLYEIYQESTKHYEILLKFHQFTSTTTAAPRECHFNNVNLQEFLGFVSKLKLLNLPNFKKKIKVPNKGDPGMGVPTRNPKIRHLQHQSNQFLVNDTDSSDSDNVTDINPQIIQKIKNKTVHLRGESTFQQKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MHP3</accession>
    <name>A0A8S1MHP3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0630024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8079967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MHP3"/>
    <dbReference type="OMA" id="YCQNTII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="157" mass="18720" checksum="E8E2ACE8B9A6F17E" modified="2022-10-12" version="1">MRNNEYDFYVDDQIHLQKYQFTLKCKYHTKISTLKQYLQKYIKKNFDIIHNDVTLNESMTLDEVFSRYCQNTIILLIKSDPMIDVLVKHSIQYQNLRKSRPTSNEGVDEILPIEIKKEKEDIKVQDLNNELDTLSTTTQKKCTNCGIQNKMIESYER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLZ1</accession>
    <name>A0A8S1MLZ1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0650116</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLZ1"/>
    <dbReference type="OMA" id="RKTFEHV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000586">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="194"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8081230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="231" mass="25886" checksum="64C94B3B37437F61" modified="2022-10-12" version="1">MNNYHYLLKFIIIGDTCVGKSCLLLQFTDSRFRNEHDATIGVEFGSRNTKINDKTIKLQVWDTAGQEAFKSITRSYYRGSIGGILVFDVTSRQSFEDVAKWYQEIQGYACDKIEMALVANKIDLDAKREVSTEEAQAFAKKHGFAYFETSAKTGENVDTVFESMAQTILKRIDSGEIDPSQEIYGIKVGPGNIEAQKKQTVTQDTQNPKPLTGYTTPAGNKKQEKQDSSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MN39</accession>
    <name>A0A8S1MN39_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0600163</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="WKNWIVL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000335">
        <property type="entry name" value="ATP binding cassette subfamily A member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000298">
        <property type="entry name" value="ATP-binding cassette sub-family A member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF36">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="450"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="903"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1066"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1103"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1125"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1152"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1185"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1263"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="537"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1327"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="513"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8078613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1730" mass="197294" checksum="B3DA0BDBDE8240B5" modified="2022-10-12" version="1">MSHLTALLKKNYILWKRNCCCSVCEILLPLFFIGLLLTIRQLFMLKTRVQVEIDDIPESSYLKFNKDADLPRILAPNLNNFPLNPNQDTELLLKSLPQLKNCIDNRDNQGASYRNGLIGIGPEENEVAQKLSTYFVDYYGYQVKWFKNNDEIDNYVRSEGYNKLEQTKGLCLAIMFETDDFNLKNFSYSLRFNTSDTTDYIEYPLNRKDKINSFKYEDQEITYLFYDNGFLTIQNWIDNLIIQKYDTSVTIEPTLSHVRSRDHIQDKFPDFVNGAYGIYLALPLMIVFLRMTHGIIYEKEKKLREGMKIMGLSNTQFYLSWIIQYLLIYGIISALATAILKSMILPESSWGFVFLNYYLFCLVLIVQSLFLSVFFSQALTGLIVSIVWYLLMFLMLNLVPGNAIPTRSQYWGVSFSSQASLAFSFGVITLMESQGNGFDGSNLTTTINNYSISIAWTWHIINIIAYLILAIYLDQVFPNEWGVKKHPLFFISWIWEKNRTDRVSHKSMSIERMNTHDDKFEEVEQALKEQEQKNEALIIKGLCRTYPNGKQAVSNLNLSMYQGQIFALLGHNGAGKTSTISMLTGLLEITKGDVVGYGFDVKTQLSELRKIMGVCPQHDILFDNLTVKEHLELFAQFKGIKSSEIQDQITKIIADVDLTDKTDYLSKNLSGGQKRRLSVAIAFIGNSKLVYLDEPTSGMDTSARRYIWEMLKNYKDNRIIVLTTHFMDEADFLGDRIGIMGEGKLLCSGSSVFLKNRFGVGYNLTLVKDSTQIESQPIIDNVMKYISSATILSNVSAEVVMQLPIDSVDKFPNLFEYLDKNLKTLHIATYGISITTLEEVFLKVAKIGAGHEQVDDVQNKEKNDEINQNVDLHIQKIEGFCSTFFLHLFALMQKRFRIFKRDIRGLICEIFVPILVVISGLAIMTVKWMKDDDLAIITPQGLYDDMKLQLFWGGNQAGQDLMQYFSNDDWNISKLSTDLEQADKEYFDQFELRESPGWYFFNQFIGSTYSYWFLQNSVFVQSSPLLLNQMNQAILRKVTNTPSANLQLSFYPFPQTQNEKSIDQSVAGYLSAFIFSIGFAFIPASIISFIVKEREINIKHQQLVSGVSVLAYWASNWIIDIVKHLIPAIVSALMVLAFDIDALILDGNYGAVFLFFILYGWAIIPFSYVLSFFFKVPGNSLLSSFFIHLVFGSIISIVIYVFFLIESTRDAASYLVWIFRPIPSFSFALGLLRTSLKQFFELIFQNSITPPNTFAMRVAGEDLLVLAISGVGYMIIVFILEFFEDNGSLQKLGSNEASIPYKPKILDDDVEREQQLCQNYKPQDQAILVRNLRKVFMLEKKQHKVAVDNISFSVGNGEVFSLLGVNGAGKTTTFKILSGELKPTSGIAYVSGHSVIDQIQNARKNIGYCPQFDALLDNLTVCEHLELFAKIKGITNTHLNELVEKKMVEMDLKRFESVEAGQLSGGNKRKLSVAIAMIGNPPIVFLDEPSTGMDPEARRFMWNVISRISTQRKQSSIILTTHSMEEAEALSTKIAIQVDGNLRCFGSVQHVKNKYGQGYEVEIKLQKPQVNLLDQIINKMGLSKGIKLNQSATLNALQKINQDQLMNFIKPKESGSHLYNDLNRHGISVETLAEYVLVESNGKELLEFIQQQLGQYEVIEHFQTFYRIRLLSNITAGKLFSAFEKNKQQLTISQYSIKQASIEQIFNIFAKQDLQQKDQNQEQVHIQVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNK9</accession>
    <name>A0A8S1MNK9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0600243</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNK9"/>
    <dbReference type="OMA" id="MDQWFEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="2">
        <location>
            <begin position="336"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8078773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="500" mass="59761" checksum="3D63DB659BE716A9" modified="2022-10-12" version="1">MSKQQQQQLSYICKDTQNQKSQGQSNRRMNNNQNNFDEKNVQNQKKQNKYNRRNNSQEKQQNSHNHQSQPVIARAQINQPQNKIYDSQTLISAYHELLQPPKEIEEFFEKIPHLFTLVSQKPLISSSIQFNHSDEEEPKWMNNNENFQIIDIENEMEKKRIEYQKQHGTFEKKQEEKSKKQNETNSEEISELQQAKEKYRQLREQEEKQLQVANETKAKLQELFTFSEQEQPKLVNSINTKILSVEEVEKQALISTSNKQKDIIKIEQPKQNTQFCFNDFNNNESDYFFEQLEEMGIHIQRKPQVTQQPIIKISREKIKTAPFSLQAQLHSRSTKESLWYYIDNFGKIQGGFTTENMDQWFEHKYLKAKLNISWEIPGKWITLEQYLINLDQIEKKLLDPKSAMDPLTLITNINNIQLNNLQQLQQINTFNVNSFQNQQVNAYQYQQINNNQYLQYQNQQMNNQQNNVNKKKNNNNQNNNNSKVQQYNRQDYQKNNQSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQ27</accession>
    <name>A0A8S1MQ27_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0610207</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQ27"/>
    <dbReference type="OMA" id="WIGNDNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46381:SF2">
        <property type="entry name" value="MAP KINASE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46381">
        <property type="entry name" value="MKPA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="441"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="2">
        <location>
            <begin position="505"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8079243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="840" mass="97862" checksum="AF237041FADF5BA1" modified="2022-10-12" version="1">MGTKLSIDKHVSNLEDILELNIKLIEKVKFDNNISNSITQHLKIWKFYEDKLYIKKVKSEPINLNQIQTNGFNFLSNECYLVMLVYKKAHEEFNQFTNFPHQMWGLVESYSNLTPRGLESPVVASESYLESFLLPQRQLNPSTSNIFEYMIFVWNGKTANPLIKAQALSNAFELENLLNKGRDPLLEILFNGCAIKNKNLSKGSILTLQSTAQTQTPNIISQNQIRQTIYLFNFLFPIPEKKQDFSKLANFIAKKQEIPYNYTQQFIQMDLPQQIQTNLQDSSQKLLLINEAQPRDYDQIKPRCNSNEIQESISPSIQNSNTHSNIYNKEDENISTSQLINNQINLPSQLRNKVPTLNIPKLDLNCKTNSQEISVGVPLIEVVENQENEKAIPMKLHLDNLKLQRDNTTYKDIEQEESNGFNFDIRDTDRKKLKIQYFSEQCSSVIPEFLYVGGEQIAYNKEILKQIGVTHVINCAGDVCKNKFPNDFSYQTYYLKDSKTENIECIFYEAISIIEDAKKNNGKVLIHCVQGVSRSVSLCIAYLIISQQITYSQAFDIIKKNRGVASPNMGFTVQLLLFQKRLQAQYDSIPISPRVFAVGRGVSGHESIVCRMVMEQLFSGKMLKIFDSRGVFLIQSEHEIFIWIGPECKQQEKFIQFALEYSNYLKQYEKAPQVQPILIKANDETQVFWNLWGIIGKPESVCKQFREWDEWYNEVQEDEFKREKEENQLLYSSDRNDRIEDVQIEKKVFYLYPNSNEYLQIFDLEDLDQSQLIILIKEINNQTLQIFVWRGQEWIGNDNEEQIFVHEIISKNYSFITETNIYVFQEEPNEESDVFLDCFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MS77</accession>
    <name>A0A8S1MS77_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0690196</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MS77"/>
    <dbReference type="OMA" id="RCSEIEP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="4">
        <location>
            <begin position="93"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8083268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="242" mass="28108" checksum="46C6F488886EE25A" modified="2022-10-12" version="1">MKPLMQYFSESDCEVNDQSICQSFRGFFDENSQINRVQSIDDQFEQISVQNSQQFSTKCSSIGEKSHKSELIDDLDDLRCSEIDLNEVNTFQPQIKCQCSKSQCQQSYCECFARGKTCNKHCGCQNCQNKQMNRKLKKIQKRLIKQKRNKQRTPRYLKGCTCGKSKCLNKFCSCHQDGKYCNSGCKCVDCKNIYKFSLKSYKSIENVEMNLMEQNNIVQFIIGSENSSKFEILNTFLKQNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MVY8</accession>
    <name>A0A8S1MVY8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0720028</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MVY8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8084370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="15197" checksum="08B5EC287B52EC34" modified="2022-10-12" version="1">MNGLVTQKKIQKIVSNFKITNSVTTDNLARGLDFDNVHLVFQFDQFQDPSYLDQVEQQDKKNMEKQFNNLNNLIEDFINSQVNQKAKQHLFHLFAVTIRDNKDKNQKIEKYNNSNYINNKYLKNKEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MX02</accession>
    <name>A0A8S1MX02_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0660039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MX02"/>
    <dbReference type="OMA" id="DEMTKYC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="388" mass="46395" checksum="8B3399082B6A6B48" modified="2022-10-12" version="1">MKCYNAESELQTTINSKQYPSEVNSPQTETQPIQKERRKWNSDEMTKYCPIDIPNAFQYDKVNGQKFQLFLDGKYSANKLVKDMNVVIKRKRLTQLEQEFHKQKEQQQLRKELVERRFTKVRTLINSNIKLRESLKLESNLKGSRMFSQLNNHIVDHIAKIKSKDLLTDRIDGVRQYEPTKLYDYSKSGIKLKFNKNQKLIKSQTQYCLKQAATLEVQDHIEQYFKQQHEALQQMELKQKRKAKLKIISDRKHRKYFTEFDINLQNKKAPPSIKDVQLFKYIHGQMLIEKFQQKQEDQKEYFANIMRSRKSYHILSNFDKPSELYAFDDCDQSFESMKEMKLSNLYAQSIELQQKLLTERRIVNQVQQSKQIDQTTNLIQIVNKQKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZ27</accession>
    <name>A0A8S1MZ27_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0740247</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZ27"/>
    <dbReference type="OMA" id="NHYVPEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8085658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="41888" checksum="61946FAC196F2ED3" modified="2022-10-12" version="1">MGAKCCAQQPILNNQVISNIQNAAFIEQLQYIPQDNQKILPQEVSAEITDEKKQELEQRKSEEDPNIFKSLQQQSQKQDIQQVTEVLQQLQHPDLKLYEQPIEITNNNIKRTLERIGKYVPNQQIQINGQVQPPYQIKGEVIYIGTWEGLKREGYGKQFWQDGSFYEGQWQNDMISGHGRIIYVDGDAYEGYWLNGNAKGFGIYYHYDGARYEGQWIGNQQEGEGKEFWPDNSYFEGQYKQGKKNGIGTFTWSDGAKYHGQFVDNQIHGQGEYTWADKRKYQGEWKENKMDGKGIFIWPDGKKYIGDYKEDKKCGYGEFYWQDGKIYKGFWKDGKQHGKGEVIDPSGNKQTGEWQDGKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0K0</accession>
    <name>A0A8S1N0K0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Adenylate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0690204</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0K0"/>
    <dbReference type="OMA" id="GTQCDRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019205">
        <property type="term" value="F:nucleobase-containing compound kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016776">
        <property type="term" value="F:phosphotransferase activity, phosphate group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009123">
        <property type="term" value="P:nucleoside monophosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006266">
        <property type="entry name" value="UMP_CMP_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01359">
        <property type="entry name" value="UMP_CMP_kin_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8083276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="23066" checksum="63C63B414EAECC37" modified="2022-10-12" version="1">MYKFSSLRKPSVYFILGGPGSGKGTQCELLSKNLKFVHLSAGQLLRDAITSNSQHKITIENCINNGVIVPSHVTINLLDKAIFDNLSSETFLIDGFPRNYENMQSWIELMDHKINFKSVIYIHCSRQNMILRIQERSKTSGRSDDNLQILEKRFITYQNDTQKIIDHYRNLNKLIEIDGDNSINIVQAQILELLNKTLIHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGP6</accession>
    <name>A0A8S1NGP6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0830222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGP6"/>
    <dbReference type="OMA" id="FADELMM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001886">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000683">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="103"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8089496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="449" mass="52529" checksum="2D738698FDC5560F" modified="2022-10-12" version="1">MQSYEKALFNVQVIRKHFFKDRVYQLYLFADELMMTDDPTKNPKYILKTNLTTTIKWICENKRIVAFEFSYNGRLKEVYGPKLNLLKEMLAGKVFFSPVSAFYQFHMEIGSGMTGSVYRCISTENYENYFAIKKVDKMKITQNDGAIPQLIHELSLLNQLQHPSIVKLKETYVDEQNYYIVMEYINGKTLYAELQSRHYGLSVNETIKIMKELLQAIIYIHNKGIMHRDINPLNIMKAETVKLIDFGLARKIRNQLIFPTSGTPGYMAPEIINFNKDKLYDEKADIFSLGCVLYKLLTGENLFTTKQGKQTIYQSNKEGVFELKKQQQHPECNSNKMDQLFILLPHMLETNPLLRLNAKVCLTILEEIENNNLYIERLIKKILIRKQLAFNTSEEQQLDKKSEEKLQKCKFRQSIDVQSKKSLDEISVFSKNVMLQNQQRVILAQKKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHE3</accession>
    <name>A0A8S1NHE3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0820137</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="QLDLQHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF67">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HUWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="4">
        <location>
            <begin position="1242"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="910"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="2">
        <location>
            <position position="1542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8088913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1575" mass="184989" checksum="CB7E0EE5C7E379EB" modified="2022-10-12" version="1">MNGLQSVFKVKGDSENHFISLSKLSLAIIYDKIIIRAQIEAQIKKTIFDQENKEEVKKTNQQLDLQHYQNENIYPICLQPAQYNIQAKEQYKISKNHQALQTLHKNNSQQEDVKYIMNLLFNESEENDYLILKNGIQLDTLNSINQNGNAIFQFNFKHKNETQTLLKLLIEQIIISYFENNEEQQYYRKIIFDVLQLLISKFPLLLPQLSRINCSQFLEKYKNKIGYENSQLPTKISFLTLITRFIIPSKNFIFCICLDTIVLYKKQNNIVPFAYEIRRLIIKQLYSEISRSINSLNEKILSISDILVTLLQIKSVAKICILNIQEPQNSYNFIKIYIEGIKNCDINKYFNQRDQLFFMITTLNILYQVAINLLLEKTEPVITNSLSVNQMELGIIGQINPIEINDQVFQNDQTQQRYQDYNSQNEQFQYYLQNWPFLSIKNGQIQVNADQQMEPDIQIHQMLNIPLINDSQEIFDKELEEELNYWTDDAQQIIEEEPENNQNIDNSYNGFINGISDQLENQIYKTIKFQGLRFPYQFQIYLQQFKQILPSQNNAQLMPNFYRWSQNLYQGNINQSPNVIRNQLVPRLGMMDQLPNVNRNILGPRLGMMDQLPNIFRNQLGPRLGMMNQLPNVNRIILGPRLGMMDQQLNLNRNQNQNFGNVQNQNINNNGVDDDEENEDMTDQIDFEMGLGYNQGNNYDFQMEYGNQQQQQQPNNQPIRQNIQPQFLQIKNIVNSVLNNRKQTLQHLGKIDQQLIQFLLKFLYVDDNYGSNYDYINTILISLGTKANFNNQIIQAAINTLKKTTQNQIQENADEQTFILSRNEIIRDFDYTSNFIQNKVFKLLLQLQSTSKQNISNENIKDCFELLFKLKGNSLKQLLQFLANQEQKYQLTLAPEQVKILYLIYGMLIKMRIKQQQQQQQQQQQQQQQQEQLDSIAKELLKLLDVLLDQVAENLTIKAVNFILAVLSNLITKNQESLNLILICIEILVKVFKKNRSCINQFVQQMNGLQSVFKVKGDSYNHFISLSKLSLAIIYDKIIIRAQIEAQIKKTIFDQENKEEVKKTNQQLDLQHYQNENIYPICLQPAQYNIQAKEQYKISKNHQALQTLHKNNSQQEDYQLKWKCNISIQDFDRLFKILCQNGREYINQIVKEQINKMIPYFKTIRQNQFRRNDYLFEKDFLDGILTYHPQVIDFQNKKEYFYQKQLINGRNQQQNFGGERMLIIRREYIFEDSYRQIKSITNPNMLKEQLKIIFEGEQGQDGGGLTRNWIYKLSEQIVDQKYKLFIPNSDNTCFIINRESSSDPDHLNKFKFVGKIFALAILKKVPIYGQFPISMFKHIVGQKLSRADIQNYDEGLKNTFDLIYNQRIDSNWNLFWAYTIKQGGVPKDVELKPGGKDIEVTEYDKKDFVRKYCHQIMAKDIEDQIKAIQDGFYSLIPKEDIKIFDSIQLELLLCGESNINVEDLIRTLRYQGYTAQHQVIGWFQNILRSFNQDMLAKFLSLATGHPKLPVGGFQNPECRITISLPNYLQNDIDSRLPTGHTCTRVLDLPAYSNEETLRIQLETALNEGGEGFDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHU2</accession>
    <name>A0A8S1NHU2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0890182</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8092030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NHU2"/>
    <dbReference type="OMA" id="QGFWMTQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08291">
        <property type="entry name" value="ETR_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF18">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="14"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8092030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="352" mass="38900" checksum="9EDABAEEBF914822" modified="2022-10-12" version="1">MQISMRAVVLEGPGQEAVVKEIPIPTPQSGQVLIKVDSAPINPSDIAFLHGAYSSGKQFPCVPGFEGSGTVIANGGGIMGWRLVGKRVAFYSQSQFGTYGEYSVADALGCLELDNDITLQEACCSFVNPLTVISMLEVAKEHKTQAVVHTAAASQLGRMMVRHFQANGVRVINIIRRDAQVDMLKKEGADIILNSSDSDFQEKLKNVLQTLRATVFFDALGGELTGKILEVMPNHSTCYVYGGLSLKPVGNVSIMDLIFKDKKVVGFWLTQYLKTKNIISQALLLNQLKGLLKTNLKTIVAKTVDVSEFKEGLEFYKKNMSEGKVLIRFDQKQQQQQQQQQQQQQQQQLQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NL08</accession>
    <name>A0A8S1NL08_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0940148</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NL08"/>
    <dbReference type="OMA" id="CIDHQNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051485">
        <property type="entry name" value="SR-CTD_assoc_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140">
        <property type="entry name" value="RNA PROCESSING PROTEIN LD23810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140:SF0">
        <property type="entry name" value="U2 SNRNP-ASSOCIATED SURP MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SURP motif" evidence="2">
        <location>
            <begin position="148"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="CID" evidence="3">
        <location>
            <begin position="255"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="626"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="626"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="713"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8093967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="734" mass="88252" checksum="D0603B7FF9143635" modified="2022-10-12" version="1">MQTNCILKPLQLQPQKPMKPLAGMQQNLNEHFNNIKNIIPYQFVDKISPHITICGLTPKITEQSLRLVCAEYGNVVKISLRAYYRDVQAQIVANITYENASQAQFAYMELQKKVENGFHFQLYYGGPCYQSTSKIVKIKLPNPQIRGIIDKLARQVVKEGVQFEQMIKQREINNNKYAFLYMQSEENEYYKWRVYSFQNGDDEKQWKQEPYYFNLNEYIYIPPSIELEEAPSFAKKELEKAQITTKNKKTQYQILDDQDRLTFSQMLRELNTQKHTIGKAMVFCIDHQNCPADLMLILEDSLLNDSIWSIKLARLYLISDILNNCNQNFKSYIQWCLPKIFSNLDQLLPYKEKILKLLQCWREQNLFDQKYLKGLELSFLMKEQSIQIESISSQLYKEKLAHVDDETLDRICRIKGLCSQGSRDTLIQRLVQHKFYNRANPDVSLEQVSKFVQVYRYIVDKDQQTIISSQKQSFTTQIQMMQEFLKLLQKRYKNIINREGEEIDAIDERIYEFNKHIEIQRAERNLYLNIDGKDLTEEDIRTIEDKKVQVVTNYELTYLQPKPPLPPVPTDPIEIEVQQYRDILFQSGLYDPFKIEEFSKSKRAQLIKADQLKKEREKQLLLKQQQELREREKEKEREREKEREREREKEREKEKEKEREREKEREREKERERERQRHHNRINKRSSSSSESDKRNQKKRFEGKYDQKYDQKHYKRRSRSRSNSRKKKAQNKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NX26</accession>
    <name>A0A8S1NX26_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0890009</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NX26"/>
    <dbReference type="OMA" id="LLEYEMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000096">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF13">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF19 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="21"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="663"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="371"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="487"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="561"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="663"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="115"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8091684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="796" mass="91830" checksum="6716E0B11C3E155A" modified="2022-10-12" version="1">MNQFEMETKKFIDELKNGVSNILVAIRVRPLSQKEKSLSEFETIKILDNKMIVLMDPEQERDEDLLRKNRLKETNFAFDFVFDQWAPQQMIYENTTEFLLEGVFEGYNTTVFCYGATGSGKTFTMIGTQLEVGLMPRALQQLFNFSIQDRFKNTQFKVCYVEIYNENIRDLLTSEDKNLDIREDKNNGIQIAGVTDVQVKTVSEVLALLKVGNKNRSKEATDANKESSRSHAILQLQVESKDRAAGLHEQIIQSKFSLVDLAGSERAANTNNKGQRMIEGANINKSLLVLGNCIQSLSEANEKGIKNPFIPFRNSKLTRLLKDSLGGNCRTVMISNVTSAVSCFEETYNTLVYANRAKNIKTVANRNVLMAQNHISNYAQLIQNLRQENEELKLLIQQQQYSQNTKQLKLPSINQKNHPVPQLSLKQQAKQLESIINQNIEDITETKNKIYEMEEQLNHFQQNIGFLQFQKGRTQDKFEQMRLQERIDNAKTQKAIIKRSQDDLEQQLLEYEMQKVDIQKQIQQIQDSNYNNYLNGIMKQGDFKIETVEIKILEKKRKYQEQIQDEQVKQLRTQINKQQAKIVQSTIQKSNGSKKIPSLPGVDSPYYQLQGGQTYSIQRYSKNKSHLKLPPVVYINQVSKSPNTQHSNKMNDPMKYRVAQKYSSSLNRPPSFHPPSSNSKSEKGKYINRSLDLGSGRDSINKNNGDLQNDISLRKLHRLRQEYQQQRFEKAMNNKSNQKSVPSFGSKILLPGMIHKSPYVRNFQNNIEPVELKKERLKMLNTNQKGQVGDKFQLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZ54</accession>
    <name>A0A8S1NZ54_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0970170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8095063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZ54"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="130"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="261" mass="30599" checksum="F9CE0D1F73584A15" modified="2022-10-12" version="1">MVNYKIKQKNVYKRCILLSSVPLSDFNKIPSFPQISLNSIQTLSNEIIKEIQPIFNNQLKQIKQQNNIKDIIKQYNNYQFNLIQNHSIQQNQRCHAIAFNKDNSIVLATCENDIKVFEFKQEQLTLTQILSEHKDFVTTLNFMKKSNQFISVSFDKQILIWQQKLNGDSYSSCCLVLNKNEDLIILGCCDNTIKFWIKQNEWLCSQTITDHTGNVCGLSLNDQQNKVISCGYDYFILIIEQTSQYYKWNVIQQISVQMFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZ81</accession>
    <name>A0A8S1NZ81_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0970156</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8095035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZ81"/>
    <dbReference type="OMA" id="KYLEPCF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045159">
        <property type="entry name" value="DCAF7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19919">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8095035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="317" mass="36924" checksum="981F98B1D890EB8A" modified="2022-10-12" version="1">MLNTNSNQFVHSKLIYACAISPKSTIDNQRIILGTIGSGGQQIIQLQSAQEGYKIKTYHLHNAFSAKLLFLNQTEGNDRFISAGEKLQIFKSEDQKISHICDLIPLSKEYVGPTFALDFHYEQQNRLISGSLDPTIRIWDLEKRKCQYQFIAYDISKEPDQYTLDLNVGRDSNILAFLNNYNQIGIYDIRTKMGMHKIKGKLQYNQVIWNKKQDNNLIAIDYEAGLIDFYDTRDFKNKYKRIRTDHRQIVKAEWFQDRNSLVTAGLDRQIILYEGDKYLEPCFGLTKNYDIDNFCCQNNLFGVVSEYSFEVFSFETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZM5</accession>
    <name>A0A8S1NZM5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0980095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZM5"/>
    <dbReference type="OMA" id="TQDNNPF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8095283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="164" mass="19180" checksum="DA4078897368253D" modified="2022-10-12" version="1">MSEEVCFTEIKKQNSSKHFPLIDLTEIKPQVNFLESLLQKKYQLNKKIQQYTLGKVYLESHNEKAIFRVFQDSDLNISSIFQKKLNKTSVDDDVMTTSSQIANANRQNRKDVIFRIFEVEKPQRPKQIPLEEMERSLFGSTQDNNPFFTQQLQVANASKLFLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P3Q9</accession>
    <name>A0A8S1P3Q9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1040174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P3Q9"/>
    <dbReference type="OMA" id="NWGSRKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="332" mass="38793" checksum="D26DB0AEC8ABD8A8" modified="2022-10-12" version="1">MQTNISGLSEECTLIVKEQKQFRYEVLDQTQLIQKQQCNSLAFDNTSTLIMASFGMILKAYLFNNEKLIYISLFQITKSTVTCIESLKKGNLFLFAMFNGQLQISSKIGINQGKYIQKLNYHLSPIYHLKSSKYSDSIISCDDWKVSFWIKQSLLQFEYFYTNNIRICAVSFNEIGDKLILSDYNKSIVTFIKRGTQWMKLQNINIGKLGQGISFIGNDSFVVQQTFKKLDLYKFKNQDNQLIKQRELNIQTYGNWGSRKFNQSYIKEKQILLNKDGISLNFIKIQDDPYFVTEFQLQFQDSSAQVALNENGTYLVTWDNYSKSINIYKYYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P528</accession>
    <name>A0A8S1P528_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1060045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P528"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8098091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="54" mass="6593" checksum="351A79F0460B8F34" modified="2022-10-12" version="1">MDQEIELPNLQFYNLYEDDDEDYNEDLNESFSNMTITQQIILNLFHLHKIPIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6Y4</accession>
    <name>A0A8S1P6Y4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1070109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6Y4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8098574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="39" mass="4715" checksum="C4F52B6D51DC8608" modified="2022-10-12" version="1">MTVGILEFEKENLRILKSRLRNKLFQSFDIGRDRKFDPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P9D9</accession>
    <name>A0A8S1P9D9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1090118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P9D9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8099381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="66" mass="8204" checksum="D6C4F26C38A3255E" modified="2022-10-12" version="1">MKQMNFLIILLYPPRIYGMLKCKFLNRKQTQFKQNHRIEIQKMAHVSMMKIKQNKYFNTKYSQNYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P9T1</accession>
    <name>A0A8S1P9T1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1100156</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P9T1"/>
    <dbReference type="OMA" id="CMNIIAI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF0">
        <property type="entry name" value="UNC93-LIKE PROTEIN MFSD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="410"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8099819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="49466" checksum="D41AED13B5614477" modified="2022-10-12" version="1">MKKYTNLGKVLYMNLVLTFHLSQCLALASLLPQIAEQNNYPSLGELGLICIYTVNLIFNLIAPLYLTKMKYRYGFVFQAVLVLPCFIPAFYVSRCDGDNVVDLICNPLMLIPLTLIGCVFLGAGLGGYFVLQNTYVSDCTSEKNKELYFGITYMLLGCAYLLNGVTSDQLLKYIGRESFFFYSGLLECGLSILFIFVQQPDKIQIYEADQKMIQNDSFGKSEAQIANVNEEFAKIGFKEQLKQIILKFQLKEMKFLFSLFISTGVVIGFEFGIFHKFISSSLPDEDEITVNMKTARIFLYVGVAQIFSGVCNGLSRSFVGIIQNSIFYGNAFQCMNIIAILSAFQQNYNINIILGLLIGFTDNSGQFNSAVIISDIWNEDMAIFGLYLFCQNFGVMFINLVAIFLDGQSLVYYLILLIILQCATSYSQQQFKRSQQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PAD5</accession>
    <name>A0A8S1PAD5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1110138</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8100116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PAD5"/>
    <dbReference type="OMA" id="RKYQHST"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000085">
        <property type="entry name" value="Histone H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810:SF1">
        <property type="entry name" value="HISTONE H3-LIKE CENTROMERIC PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810">
        <property type="entry name" value="HISTONE H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="9">
        <location>
            <begin position="45"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8100116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="137" mass="16109" checksum="27565F273BFE8234" modified="2022-10-12" version="1">MARTKQGKPNNNKFSLSPQSFCNDSLDNKKQPYKNQQKRKHKFRPGTVSLREIRKYQASTQLLIRKLPFQRLVREVSQRSEKDFRFTQSALYALQEATEGFITHLFEDANLCSHHAKRVTLLTRDLQLAIRIQGIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PB23</accession>
    <name>A0A8S1PB23_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1120082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PB23"/>
    <dbReference type="OMA" id="MIDSFKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="282"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="341" mass="41082" checksum="44DF2C0AEB4F1876" modified="2022-10-12" version="1">MIQKISEIFKQDSEFIRFLKKSSLSKDNQVYEQEEIDFLLQYMTKEKNLREFFDFYSNFDMQTYNIKMKMLIITHQFIHSNEEFSKQFTSLRLTNFYLLKHSHRNYETWLLLNIQIPFLSYLQKLAISFKEIKSYKTSIFRKSNYQNLMIDSFKMVNIVNMALSLVPNLKIALQNYPYDRLLQKLTFNIYQEIQGYQKNLINSIITLLNENSKIQSVDLYELLRELKVIEQKMILYHQFRNQYDQKEILQSPLQIQIYFENQSKSSIFQNNCKAQTKREYRQSSSRTTRQSTGEEATTPRSQRFLNYLDQQYSSCAQLLLKDKDKIIQEESETQIDQTIQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PF55</accession>
    <name>A0A8S1PF55_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1160132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PF55"/>
    <dbReference type="OMA" id="TVNESNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="121" mass="13733" checksum="B931EB5FB1BB0FD9" modified="2022-10-12" version="1">MSQTEFIELHQSSQVQNKLKPILKHNRNTYLTTQTRTDIAGNQIIKGGNHKISFLPISNEQSPNVVTSSNIRIYSESSESDQEINESNSTLHKKPFLNLKGKNCQNTQTIIVNESRCCIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIL0</accession>
    <name>A0A8S1PIL0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1200040</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1200041</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIL0"/>
    <dbReference type="OMA" id="FQLETYS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8103114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="33172" checksum="2617D243A2907A03" modified="2022-10-12" version="1">MGNKLQQSQQQLNSYPQESAFQFSNQDQSLQSRLTHQLIEINQALQEPAITEYQDNLQNNAFSDLIHQQNFKFQKKCILKVLMIFLIWSLIQFFILYILLKRGMFNLIDLISPYLFHISVIFMYGMAKFGTLKFSRKYPQNCLILLLQVYFTTQTYITFCRSFSNERIHVSNGIQHTTADFVTKYAVQQFLFNILITSTLIIYIIIEKQRIRPIVAIALCCVSSLIPLIFEYWLFINSIVASLYGSTLVVVILQILKGRFELETPNIITATNILFYGLISPIDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJV5</accession>
    <name>A0A8S1PJV5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1210034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJV5"/>
    <dbReference type="OMA" id="NDTPEIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="467" mass="54768" checksum="606EA968536EB06E" modified="2022-10-12" version="1">MSNLSIRKIEYLVEKYIIVQSSIEDLMDSCEILILNHEIKRLLPFVAYNESNTLIQELMKSFYRKLPQQDNISEDKEPENTIKDNLQSAIKVEILPEPQQQFITSVTKFRKASQKSSMKSSINSFRKSSLNKQERQQQTQQGSLHITFSPIPLQIEQEEDDEDRFYRGVYEMKKKQQEQIRFNKHVSEQQKVQIQQQLLDLKRKCKNGVFTFDNDGSIILTKQKAFSNELQVHPETKICALNLKNQGQKLNLQTQITNIVNSSIQRKQRLAKIVNNVKSDIRMETEPVRISTSFKTLNNQDFKSKSILKQLLNDQSLKLTKKEFQQLAGLENNIGDVLQQKLLRMTQIQLKRAQYIQQQHSRSITPLINEEHKEIEKPIIKKNTQQGKIKLNDLRLADSISNDTPEIYFKNISESQESKNNQNIKLMKQDLPFLQMNYKQSKYAILKEKKHQKINQSVKLRSYTTHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKU5</accession>
    <name>A0A8S1PKU5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1210031</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKU5"/>
    <dbReference type="OMA" id="GIMENKN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd09487">
        <property type="entry name" value="SAM_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="2">
        <location>
            <begin position="17"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="1">
        <location>
            <begin position="185"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8103404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="42490" checksum="D5001107381977B9" modified="2022-10-12" version="1">MDYDDLYKVLPKDPKKWKLDDVTIWLKFIGLQDLDVNFRQNSVDGALLSTLDDNDLKEMEIAESSLKIKKLVQWIKIGFKEYSEYLKQKEKDQTFDSQKQIINQQYDENNASNNLVVSNQFKRSSIKAAIFEDQIQSNQSFKQTVQEVNNVQEIIKSTQIKQECSPYLFNINNGNQRIQIPKEGISIGRNPDNTLVLKEEYVSRQHCKIFYKEINGSFYLQDLGSSSGTYVQLTNPTLLKEGLILHMGLGQFLISKIKIQGNKCSVFIRVLEGIMENKNLDFELTQDQTALIFGRQMSMFVDDTHLSGQHAQFTYIEEGLVIEDLDSRNGTWLRLSPPHKQSEPVKLIDGRRFRLAFEQFFEFHTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PND7</accession>
    <name>A0A8S1PND7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1230049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PND7"/>
    <dbReference type="OMA" id="CYYQKGS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="102" mass="11590" checksum="1F2B343E0E91795E" modified="2022-10-12" version="1">MSTLTQLMEVRSEKLLKTQGDSDSVIPDSDQEENLRSPFQKGTPKSTIKPPKRLRFSGMNQVCYYQKGSKRDMSPSPTRNTSLSTHSILKNKDSPILFKENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSA4</accession>
    <name>A0A8S1PSA4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1280152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSA4"/>
    <dbReference type="OMA" id="FRMQQDF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="234" mass="26896" checksum="41446DB0B31A648E" modified="2022-10-12" version="1">MDFKSRFKGKMTIMNKDGVIGMVNKPSIPIAKNNQYLASSDEISPSIQKLGHNTEGIRESKSHIFTNIQTSPNQGNSTNYAQKFSNAGSQVVKQNHIKTEHPYYFDDPKKQSNQYQFRMQQDFNQLDMDEYEIVAIPKTMLGQLRQQMSVDRYGLTRSFGPEKKIPVEFSQLSPQITTQCTSYNQTPLLKKQNTIDQILKPPLNKQKSEKMEKKNQTIQQAGIGTLRYNSYSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTD9</accession>
    <name>A0A8S1PTD9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1310007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTD9"/>
    <dbReference type="OMA" id="CIKFFEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="290"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="156"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="290"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8106475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="663" mass="77545" checksum="DBC9C1A860323BDD" modified="2022-10-12" version="1">MNLAISSSSEEESSYNAKEQDRILKNSNIFKQKANSHLNCLKHPNKKAKYYAQNDNRTLFCSKCALNLALKGHRIEESAVAELELQRQMRINQFQEMMKQALLVCNSKIICLNNLQISQKQQLEDQKINCIKFFEQVIETAQQLKQTYIQKFQHDHSSLEQNILDCQQAVTNYEKQLQQYQIDIDKNHTNIVKKMEMKPFEDIMQRYEKRVRSIQQLLSEMKLESGVKINQFEQSSILTFMNKMCYNILLNDTSQSIKSRNNSINFNNPSIDMKVLDILENDDIYQSTFSNTIKDSNSQNPPSPISKLPIYKSNGSNPISTIQNSRRESNSNTPESWNHTNIQNCDQQSQINQQINSHNVSLNEQPIVINQQQQIQEKFSVKEIVKLANENAQNVEQPKNLEITPLIHQKSQSIMPQSIKIHNHQKNQYDLNTINKSQDSKQRKKQPSVDQIEQKRFYILNQNQQKSQLSQEDTLKDRIFKELSGHSSESVYNQVLKINTLQQKMKKGKENTQIDWTVKYISKKMSNQLIDNILFSNRKLYFINYIYVILPKNNKKEIFFIIWFAYLTLGNSIVGKKASEQFFPHIMDWLKKQEQKVDQVLAFSALTEIIQQTDEELFQNTVNSEFIKSFQQQKKKSLSMLGQKVQRNSFKMNSSIILTTSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXP0</accession>
    <name>A0A8S1PXP0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1350077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXP0"/>
    <dbReference type="OMA" id="EQVPNIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="13"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="349" mass="41764" checksum="30D1B69108B13C8F" modified="2022-10-12" version="1">MENNRFYSNQSCQRELEQQLKDSFLEIKLLKQENKYLSSTFDEFKLKQEETRKLQSLVVELEQRVLSLEEDNAKLEAEIGYLRGFSQSKEIQVNQKASYAQLEQELQDLQIYLRKFIQLQEFLIEQVPNINLQLCWKEINQIFKNQFNTNQQIQNLQDELKLIETTYKELVHSKDNNLTSLQEMAKQQSIRESRVEIERIRKEVQLKQMDVKQSETKLSDLQNEFTYISKIETRKNNVQLEAEKQLIEFRRGISTTKNSNHNVSLLRDTFKERDTYKEMIPERLRDQSDVLPVTSSIKRLNRPYIEINDRFDDYQEATKTPIEKTIDQTRKKLSRQLSQASSLRDQKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYZ8</accession>
    <name>A0A8S1PYZ8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1370069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYZ8"/>
    <dbReference type="OMA" id="ISVPNEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="187"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8108456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="550" mass="65369" checksum="DD948487D9D70564" modified="2022-10-12" version="1">MKPSKSLFNDLELPLWELDENLIFKYKVQAQSQSLNQILKTIFNKQSDDRTYEEIIFINEYLLGFTYFRKIKNFHGQECLFRLSQFAQLISVPNEMIIVKKNEEPNAFYFIIEGQVMQINETQCNVSNIADALFEENLTKLTPNKSEYITKKNSIFIIINAFIFRASLHKYQHQINPKKMIQINHPILNVLQQYDIEELSSDAKIEYYEQGQKIYKINESLEFLYIILEGKLIIGKKVGSIQRKHFQQIPSIFLAESTKEKPQVKPIAEISENDIFGFEEYISRQISREFQVQCLTKQCKVIKFPLKIFFSKFNQVFNLKEFKLLLDIISKRNHYRNRIHSIQVQLYENQSQSTQRSTKPMYYETLEPIPYDHQIRSKPRSFLLENSILLPDDLISNLQLSYKLRTNIKKNDQSINCSSFTLENVTEKQKEKKKLQIRINYQIERELHNNKSFDKTKLIKKQEKIQKQPNDQSHIEKLLNFKSESKYGSVKIKQEVIDQSFQLNQVRLSQNSRYSPLPEIPSFQPKQGKFQQKLILKNFKEKQYLIIDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q041</accession>
    <name>A0A8S1Q041_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">P-type ATPase A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T1360094</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q041"/>
    <dbReference type="OMA" id="QIEIAII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140358">
        <property type="term" value="F:P-type transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF8">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="965"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="995"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1034"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="12">
        <location>
            <begin position="218"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8108269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1068" mass="121474" checksum="D8B81FC75BEB8BBD" modified="2022-10-12" version="1">MSYPVKNSDIEGQHILYQVTPVQLSKIRLLLAVVFSICSAFIFALALRYSRKFYNAFLFTQCQDESVATHYQIETVSGEQFVCAKKFIDNKPHFIFRMLKYRFNEQIKSYEPIEFDAKEKVISEFLQSRNTLSRELKIQYFGTCQLKIPIESIFIYSLHAFTGPFNILQYFAVAIWFAEKTVLQPVLILIFTVLTVYLNYFLYVRSRRRLQQLANIHQDVTIKEADQFKLINGADLVPGDLLILKENQTLNCDCAIISGDVIVNEATLTGEGIPIPKSALPNQNSIFELEKMSQHCLFEGTKLIQVNNTQENIAIVLRTGFTSLRGQYFRNVLFPEPPSMRFYIQAAKFILEIAFIIAVVYGFLLIEYIPMEFKSSILVLRFLDNIVWSIPPSMPIFFQICKTASLVRLEAKGVIGNNADKVESAGRIDTCCFDKTGTLTTLGFKAIKAFPEEEKPILDAIMGCCHHLIKLNGQLLGDPLEIEMLNFVGWQCNFHNNPVTIEGNGKIYTIHKIFDFSSQKSMMSVIVTDGSKYYLYAKGSPESINSISIKKRDDLIEEFNLNAIKGYRVLGLAYKELQQNQIDQQREQLEQQLNFVGLLVMENPLKSDTFDIITTLKETGLDIKVISGDNPLTTIQCAKLAGIVNQDNEITFLDYNQSNQEIILQCNNNQQIIKESQPNSIQELEQFLSTQQELALTGKFLEYMFNKGNFLIESKNSQNSFGKQQSKEKIFTEINQIDIDDGSFAQIAISIIKKTKVFARQKPEQKKLIIQIIQSFGRQVLMCGDGANDCSAISQAQVGISFSEADASYTAPFSSKSTSLDCVVKVLLQGKAATMTIIEVFQYQISVNTLKFIAVLFTFLEAETFADFQFTYTSIISNIPLLIFLCLSGPCDTLAKYNPLDDQFAIYNQIQIYNNLIWGAAGLIVNFFISQAVRDVHTCKIDEPIINCIPKPIDELKKSGDIQSVMFMSLMFFFMTFAINLYISNPFKQRYFRNYLLLLWTLLGFILFFVSAIFPKGGKWAKLINVNDNAFQGYNWIMIAVVVIASLLGFITQAILQKYLPSKKKIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1Q5</accession>
    <name>A0A8S1Q1Q5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1380069</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1Q5"/>
    <dbReference type="OMA" id="CIMITSL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="259"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="614"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="625"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8108809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="740" mass="85394" checksum="1585AA5F4DA42662" modified="2022-10-12" version="1">MQHLSRPQLQLQEIVQQNNFKTCTNKSNKLSQSFNPCGLIAQSLSPTHKANQSTGLQGIDLRVLKKQGPPVQAKQYQQELLKLMISHKQLPIQKSVQKVNTSDTSNNLKSNITTDTRRTNSNQYQNTNAQTTKNQGFINLANQIIIIVHYENQQIKFPIDPSKTTGWLDEYLKQEIKKHSIHQYSSTGSQPDHVKITCGTGNESTNPEIVSFHTIDKNLPVDYYLQQTNKPLDIFSGQTLNLQPFFGTPQTSKVSLKDFIFVKCIGVGGFSRVYMVKKKSNGKFYAMKLIDKEFILQHKKQGIVQNERDIMTVLDHPFIIKLEYAFESKNFIIFVLEFCSGGELFWQLRQVKRMTEYQARFYFTEICLAMFYLHSLSIVYRDIKPENILIDIDGHIRIADFGLSKPNMSEEDYAYSFCGSPEYMAPEMLLKIGHNVQVDHYCLGALLYELVTGLPPYYSRDTEEIYESILNEELTFPEKLNLSNEIKDLLKGLLSKQPSERLCANKGLLELFTHPWFKDCDLVSILLKKVQPPFKPNQLQFNYDSNDLMNGELETREKLLGKTGLQQEIKIFQDFYFDQSEQKQMKIEQAKVLKQHCIMITSLQMQLLQKFKSIRKSTEPKESSNSKPLSPYTGSRQINQKQKIQSEQMSSLQKRIKSQQSSLGNVQPIQPNCIVSPQQSKIESLKRIISQNNFFMDRQNTLPTNNQQAGQSKDSIDSQLIYQNSQNMHKRVFSLKQRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-05-03" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q3S3</accession>
    <name>A0A8S1Q3S3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1420054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000146">
        <property type="protein sequence ID" value="CAD8109923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1524"/>
            <end position="1574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1601" mass="188446" checksum="1F6464895A6954D4" modified="2022-10-12" version="1">MNEEKKKLLLEAINNFSKNNDFAKLRAILAKFKRNSSVTAVQDRFFAKLFATQFGGAIVAFQKWKNLPEPVNTEQLKNARKFERKLDILFHTHLKVSFDPLKEDYMDAQNIKRMCLRKIFEKSMSAHKRLFLLWAQQNRQAKQIELCKLTTSLFVGLAQIVTANVQPIMQNPREAQTKEKALHLIFQGQTENLLRAFFKWRNWAQQERMNDNFLNEIQSEQRKELIDRLQGFCNGNKLNLFRLILAKFSLAHKKERLILQINARILRTQIGAVWDTFNKWKNLPEANAEELVKASKFEAQLNKFALHIWRKRTWNPLQEVYDDAQALKKRAIILMIKTQESEQQRALDQWNKNVGLIKEVERCKCVIGLFGFIGLHIKNNIAPLKPDEESQKKEKALLRLIGNFDSNLRYFFMKWYNDGKLLKLQGAMNEEKKKLLLEAINNFSKNNDFAKLRAILAKFKRNSSVTAVQDRFFAKLFATQFGGAIVAFQKWKNLPEPVNTEQLKNARKFERKLDILFHTHLKVSFDPLKEDYMDAQNIKRMCLRKIFEKSMSAHKRLFLLWAQQNRQAKQIELCKLTTSLFVGLAQIVTANVQPIMQNPREAQAKEKALHLIFQGQAENLSRAFFLWRNKTQQDRMHQNFEDALTVEKKKQLTEQLVQFEHGNKFALYRAILAKFSLAHKKERLILQINARILRTQIGAVWDTFNKWKNLPEANAEELVKASKFEAQLNKFALHIWRKRTWNPLQEVYDDAQALKKRAIILMIKTQESEQQRALDQWNKNVGLIKEVERCKCVIGLFGFIGLHIKNNIAPLKPDEESQKKEKALLRLIGNFDSNLRYFFMKWYNDGKLLKLQGAMNEEKKKLLLEAINNFSKNNDFAKLRAILAKFKRNSSVTAVQDRFFAKLFATQFGGAIVAFQKWKNLPEPVNTEQLKNARKFERTLDLLFRGRLKASLEPLKDVYQDGNNKKLYCLRKLFTLAMGQNKRLFLYWRDINKLHKSIETCKYTTNLFQTLALTLAGHVQTIFKPNKKQESVLNKMLMNYSNALRWAFAHWNNQAKQSKIQSQLDDEKKKLLLFALHRNLRTNDQGKLRDILRKFEQARQKQALIKKIQIQLLHTLVGQIEVSFQKWKALPENKELDLMKASVFEKSLNRFKIHMVRKTTFNHLYNIYLDGQAKQKYAVNKMLYSCMSAQKKAFLKWYKIIEFSRAAEAFNISDKKKLAIQLFYQHKMNQLNVAFVEWKRLSSNLTNKKESAQEREIRLKREAILLFQNFAQIKLRIYFQRWNIRAVKKNMISVFNAIQKLLLFSMKQDRELMKEALNIWRGPKLQNQWFQRVAEMIAKNTRITPQIAFWRMRDNATTQRAVSLNTLQIVKCKKLINNLLKAYDRVRQRAFTNIEHFGRGITDASSFQPSHSSFLQQTPVRDSLAKSQVESILLKNSQQVALNTLQRVFRRHLKLRFTELVLIASQCQKLGEQMLKSSIQSTQYKFIHRKMATEKLIGIIERNVQLKELVFFNSIANQSDPDLLKRMNELIEENSRLRDQATNDETINSKDQQISEQQRLIQDLNTRLDRMRGQRIIKSLEDYEDHLVEDGFLAIKESKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7W3</accession>
    <name>A0A8S1Q7W3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1470088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7W3"/>
    <dbReference type="OMA" id="HIIPIGM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="159" mass="17959" checksum="1FE35FEE835C7606" modified="2022-10-12" version="1">MSEIPETITCTEDFTPMRKGIFVELFLLYLIYMVISIASYYQNPEQEISQPVQHNDGAAPPTQSQVKPSLISVEALNKFFTIFFGYHIIPIGMALLYQPRIYSIVICYLHFVAYIVIMVAYQKEKESILKIAYAASGLFNILLILGAIVGLSQTEYGCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q806</accession>
    <name>A0A8S1Q806_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Choline transporter-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1470118</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Choline transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q806"/>
    <dbReference type="OMA" id="FCAIICK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF14">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="514"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="556"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8111443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="636" mass="74162" checksum="657E57C8A783070A" modified="2022-10-12" version="1">MIIDPTFLVKITFYASMVIFISLTFTSILFGQPDKLTRGFDPDGIACGADFGSNQYPYIYFSNPTPTTQHQSVCVKSCPKPDHKNGMPKQLECVPNSNVQQCQAKFSIDNPEDQFLIYDTFLYKGNICMPRNLAYYEAIKDIATPPSRLTNTSDVFQNKWMLLIFIFIAGIASKLLLTQLKANTQYSVWALTFGLFIFVGSLGIIFVSQAREAISNSSETNSISSFQVDEEYILKMTNVPDPTKLIFLSLLFVILTFYGAYFLYHNYDRIKNLSQLFEQVEKFMAEHEYLQEFSYPTILVLNFFLFLTIYTILSLRSCFVIDFEQIGPFEKIQGGFLYYFQYLIFIFFLWGVQIIFGFNNYIISSSLVQWVQVGHVHRQEQDFQYNHNQSSLVLTRFFLQEAFYNLGKIAFASFLLLVSPIKFVCDALKDWAIRRKNEKFRNFLTKYCCLPFIEIYNKTRSIEEVIYVELTINRKSNVSINVCLKFIDDYLELKQEDSNAAEIFEQLRNTVDSFLLITKIFIALFCAIICKFILNFKYISKDMYETNLTCLIAAVIGYYVASLYFQTYSIVLQGFGYIYLRTMHICKMNNEAYEKNQQKKKDTAFETFKSIFKDFSDIMINFENKLKEQKNEQLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8Q5</accession>
    <name>A0A8S1Q8Q5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1460041</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8110990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8Q5"/>
    <dbReference type="OMA" id="RELVIMC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF6">
        <property type="entry name" value="SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="6"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8110990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="364" mass="42462" checksum="848B3A0AEA8AF03C" modified="2022-10-12" version="1">MSLKDFQLLSKLGEGAYSSVYRVKRLEDGYEYALKKVKLQNLNDKEKQNAMNEVRLLASVKHPNIICYKEAFIDIQSNSLYIVMEFANRNDLYQKIVNSKKNNKQLDEQSIWNIFIQIVRALKALHELKSYIEIQNANVFLSQNGDVKLGDMNVSKVVKKGLLYTQTGTPFYARSEVWKDQPYDQKSDIWSLGCVIYEMAALKPPFQAEDMQGLYKRVIRGYYPRIPQQYTQGLTNAIRQMLQVQPNLRPNCDKLLQYPSILKRQDEILQLEQISNEPNTLLLTIKFPKSYHYFTSMLPKACYDFVNRRQIKSLHSQQVSPNPRSIISVNGNYEDVTSQKSIHKQCVNKPQKEFKMILDQHQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QE44</accession>
    <name>A0A8S1QE44_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T1580010</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000163">
        <property type="protein sequence ID" value="CAD8114068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QE44"/>
    <dbReference type="OMA" id="WDEVCES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17940">
        <property type="entry name" value="DEADc_DDX6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="68"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="247"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="409"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="588"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="7">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8114068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="749" mass="85136" checksum="223DC3E1090C0190" modified="2022-10-12" version="1">MQQSDSQQPQTTQTIDEQPKPKDTRYKTSDVTKTKGVEFEEFDLKQELMQGLAAANYEKPSPIQEESIPFALAGSNIIARAKNGTGKTGAYIIPILEMMEIEGQIQSLILVPTRELALQVSSLVKEIGKYMKVECMVSTGGTDFKEDIYRLKQVVHVLVGTPGRILDLAQRKLADLSKLKHFVLDEADKLLSVDFQPLIVKILQFAPPEVQIMMFSATFPVDVKGFINEHVPQIQEINLMEELTLKGVTQYYLFIDEKQKVNCLNFIFSKLEINQAIIFCNSARRVELLTQKITEFGYSCFYIHAKMNQKDRNKVFHSFRKAVGRCLVSTDLFTRGIDIQSVNVVINFDFPRTAETYLHRIGRSGRFGHLGLAVNFITETDKDTLVQIEQELDTDIKPFPKEVDKKESIPFALAGSNIIARAKNGTGKTGAYIIPILEMMEIEGQIQSLILVPTRELALQVSSLVKEIGKYMKVECMVSTGGTDFKEDIYRLKQVVHVLVGTPGRILDLAQRKLADLSKLKHFVLDEADKLLSVDFQPLIVKILQFAPPEVQIMMFSATFPVDVKGFINEHVPQIQEINLMEELTLKGVTQYYLFIDEKQKVNCLNFIFSKLEINQAIIFCNSARRVELLTQKITEFGYSCFYIHAKMNQKDRNKVFHSFRKAVGRCLVSTDLFTRGIDIQSVNVVINFDFPRTAETYLHRIGRSGRFGHLGLAVNFITETDKDTLVQIEQELDTDIKPFPKEVDKSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QEU5</accession>
    <name>A0A8S1QEU5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1600010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1355" mass="160870" checksum="E97DE0F13619ED08" modified="2022-10-12" version="1" fragment="single">MSIVWVNKYQMKLNIILYFLVNQLLFMINQYQTLTKELRYILARIILSFYYQIKMKSFFVFQIFLQVLSEAQKNQGEIEQISIYPTQGEIMQVPIQGFLEGTFFSFEYRPKQPFLKLIDVYKKTSFAKGDSYRSISSNGTHFALITNDQNIIVYRWENQQIEQVEPIYAISEQCFNTLFSYQINTIIVDCYYLNNLEIKELQHGNQKTLYQTQSQLPIKTKIKQIINGSNYFTVYAQFFENYSVLTLFSLRFVNITSWISDFIDFDVSQKGFNVIYVLSLNLLQQIKISDQYTFEINANSQNFDFTDGKVQFFSVYYNDIINSQCDQIILVFYSKRDIFINKKYGCLDSILDPNEFEFYKIPIQQGQPLQITFNDQFYLIQMTNTLLISPTFWNSRYFIEKIKNSSLLYFNTKTNELFIFDRIIETFQLNFPYLEINLIQYQPQLNTFLIEIYANVYILPIIPKINIKIQILNQDDANVYVITYKNFIQNQLLLGNSLQTKLSGFSGQLLQYQSNQNDLQFGQFINVNLLKVYDFYPLQFEFAQLLNTIYYFQSISLGNFGNYLVGIKDQILIFYYCQQYSYDLQQKKKYNCTIIDQTPIFANVSQLQIGYNYFSYVVAGIQYQQDIQIFQLNFEKSISNQTINLEQQFTQYLINYDNIIGLIEYKEIQIISYTMNNKIVLNESLFHEIFQLTNISFQPIQIASNVQYQSAILFINNVNNVVIVAINGNNDIIPKSIIRCQFQISSLNIVNQKIILSYQCNNQTSYCFQVWNFNNILSPFFEKELLSIEYKYQFNFMSDNLFFYVTLENKTIFIYNPYSSRHMSLFKKLELDYIILSTTTNSQSSILFYNNTILLLSSIEQELFKVNESLIYQNYYPLMIYRFQVTSYQILLLSNIHLYKVLLTYQILQYYKMLLNKKQVQMLQISIINSIHLQNRCYLFDQNSKCFLNNINSSLLLTQNNFSFTLITPINNNFFVLQSNLQITILNSQLEYNCSKTYDYSSYNFSECLISTSKGNQLFSICQNSTGQYYIIFQIDSLGYATIYRINLLAQQFNKLIKISFIDNYCFILGDDLLYLLNIQNEEFFQRSYQCEDFSAFPLYQINFIDNSQYDIIVLIFIQYYELYYSIIVLGENQYFQNKVDLNYVEEIFNDNLQFLFSYITILDIQRSKILLITGFINQTSWILSLKINLEKQILSFHNIVGTIPPYGNYEILKNIQYQHGFLAMQLIYGNSQFMILIYDLSDLTNQEMNQPLLIYQNQQNLSTASFGMNISKEFKNGTIITFKNNSIYYQFINTYSITCRFRNKQTQINVKMICYNDFSRGEFKFQYQLPNLQKKKQHWYYILLSIIMILLIXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFC7</accession>
    <name>A0A8S1QFC7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1550074</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFC7"/>
    <dbReference type="OMA" id="HHEQIIP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="530"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="571"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="599"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="4"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="422"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="530"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="578"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="603"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="637"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8113467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="658" mass="77177" checksum="418589C0A3A87475" modified="2022-10-12" version="1">MSKEQELQTKIKSLEDDIQNRHRQIVTLTDKVEELQRSIQNNSEVVELGEKVRMLEQQNLKLIEKNQSDVVEWRSKERDFNNQIQTLQRKLNEVEVQLSEAKDLNQQLIAKSGENTNSESQLQQITNHYEHLLSEKDIQLKERETKISHHEQIIPQLQKELNEKDQQIQSLNEQLEKQSQQLILITQQNVNINTLTDELNNLKLLKDNLLNDNQTLNSQLQQLNTTNGDLLNKLKLAEDSNTTYQKQILELQNQLKDTQKEYKEEMTKIMQILEKTKEKSTNEQELFKNRLLEKDDILNEAKKQFEQTTKQFEKENATLDTQLSQANKTLKEQTKKISDLELKIKELESKLQESNRQYIKSLEHSKQLDIELKKSIQISQKHQEQIIFKDEELQKSKQLQLRILQDNSDLKSKLESLLQMNSKTKSNELQNLKNQILEKDQEIELLKQQQRSLATQLKTSQGYTNRFKQRNKQLEMENTELLKSKNNLETLFQEILDQTKQKSNVKNLSALSNNPTKYQMSLLSQRKNYMQENTSTHSQENRSSSQKSNNTTPNKVGKTGDVSFKRAILVSGQKKTAHQGSQDNVNTKQSNKQLNEIQELDESQQQQNKPQSSQDNQDQQMVQEQQLNLQVHKEEQILDEQENNEDVGDFIDDDNDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QGT1</accession>
    <name>A0A8S1QGT1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1620118</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T2070005</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8115119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000216">
        <property type="protein sequence ID" value="CAD8117697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QGT1"/>
    <dbReference type="OMA" id="NPNMNYP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8115119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8117697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="281" mass="32364" checksum="3050457A866A3E99" modified="2022-10-12" version="1">MQSPLNTDDSIGQNNFYQMADYIQSPNQTTCLDILGTQVGIFVQQRFEFFQVMMGFQTPNVFKVYAANDQGQQIEQKELFKCKEKSSACARLTLGPPYRPFEMFVSNYGCKDFQPNMDQLFQENSDRIVFRFDRQYACTCCCLNRPRLDVQYVENGQNKSLGYIINPQYCCTLGCHIYDSEDKLKYIIEGSCCQCYFWCRCPCSDQCNKVDFDIKTSNGEVVAPMKKEAKGCCQNWADFTDNISVVFPQNASKEDKALILAATIFFDYMYFENKDGPQPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QIU7</accession>
    <name>A0A8S1QIU7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA 2'-O-methyltransferase fibrillarin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Histone-glutamine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T1630068</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="14">S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-glutaminyl-[histone H2A] + S-adenosyl-L-methionine = N(5)-methyl-L-glutaminyl-[histone H2A] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50904"/>
            <dbReference type="Rhea" id="RHEA-COMP:12837"/>
            <dbReference type="Rhea" id="RHEA-COMP:12839"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000170">
        <property type="protein sequence ID" value="CAD8115266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QIU7"/>
    <dbReference type="OMA" id="EKQEYRT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000001">
        <property type="entry name" value="Fibrillarin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000386">
        <property type="entry name" value="Fibrillarin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020813">
        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8115266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="278" mass="30127" checksum="4A8550A3FA869F60" modified="2022-10-12" version="1">MGRDFRGGRGDTRGGGRGGDRGRGAPRGRGAPRGGRGGRMGGAPKAFVVPHPRLAGVFVAKGQQEALVTKNMVPGESVYNEKRISVEDKQTGEKVEYRVWNPFRSKIAAGVIGGVSDIFIKPGAKVLYLGAASGTTVSHVSDIIGSEGVVYAVEFSHRSGRDLVNMAKKRTNIVPIIADARKPLEYRMLVGMVDVVFADVAQPDQARIVGLNSQYFLKKGGHFMISIKANCIDSTNRAEVVFQHEVQRLKDEGLTPQEQLTLEPYERDHAIVIGVYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJF6</accession>
    <name>A0A8S1QJF6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1610054</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJF6"/>
    <dbReference type="OMA" id="YMATYLM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000121">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="237"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="156"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8114750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="423" mass="48936" checksum="69CFC729DBBC323C" modified="2022-10-12" version="1">MDNIYKFKASSSVGHLKSLNNDKQFYSEEESSKKEEEEKQLKKQSKPPSKMSQFSKNATGYQTDDSDKETPKNNKIGPSSQKVSKFRNLVNAKKIEGDNDDPNFDDDDDKPTTIQQQIQHVQQQHEDQYGVEQQASKMQQKYAKGDVGKSTKMKHSNTQQTMEANSDVEQMKNKQHQEGKLKNHPFRHLIYGPSIGENSFNKFLQLTQRGLVYARKCLKGPSEKFIKSKMVQLQECPIVKAKTLLLDLDETLIHSCSLRENPQVTVTAFGDYGEEAKIHFNIRPFCTWFLQQMNQLYTIYVYTASSSAYANAIVNYLDPKRQWIMGIMSRGNCMETKNGFFIKDLRIVGNKQLKDMVIVDNLAHSFGFQIENGIPILEWHNDQNDQELKYMATYLMEAAEQEDIRQFNTQRLKLDQLIEYNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNP5</accession>
    <name>A0A8S1QNP5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1700003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000179">
        <property type="protein sequence ID" value="CAD8116177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNP5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="275" mass="32542" checksum="CB93A381D127A5A0" modified="2022-10-12" version="1">MQVQKLQQLKQIRQKYTKCLETISNQDVLLCKGIIKQWISNIIDPLIKSCSTKQFILQSNENSKNPNSESSIQCRQNQLMLLTIQILNNMEKFTEKDKIPELFQTSLFLSLFKSQNIKAPLFVAKRTKYYTKNTIKIGIYQEKMIISEYFIFRLLISNLIESSNNMIYQNINHKMQCKFIILAIMGILQILYQDYFSELKQLDQPSTELFQRRIKISKQQDISIIIDDNIDQEESLIFGLHCKKHFENLMENNQEWLQEICLKFAKILNNLHSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPS8</accession>
    <name>A0A8S1QPS8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1760015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000185">
        <property type="protein sequence ID" value="CAD8116637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPS8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="484" mass="56483" checksum="676C58D785D965E0" modified="2022-10-12" version="1">MEQEVGKICLVEVPIEKTFLFKIKLQLKKTIQHQEKDYLIYQLKQKLFIVCPPSYALKELDTKINNKTLFDGKGYIKAVPKADYPSIVGQQSGMISAIIQQNGKGYRLKGCGNGNDCITLRKFEGDIENAFKIRGQLFMNRKIEQILKKYNIETGNISIGYWQYGDIESINQNLEKNLQNQIPLVPKYCGIYETIGDFRLGTHLLQGLRLIVNSSISDWCLDDIRRLFTVQSRIVENRKSQNGNPWDLNNTQYRERPEGTILQEDDCQNNRVFDWEKEKDNISIIENLIGNSMLQNISILYQRIGYEVGKIKRIFIDNDISWGYFRDHSKFYYHSNAHLDNFVIIQNGSNVLAPVDFDLAFCKEEFINIDIDFDAQRANSSEISENKNYGKFDLEQWLFYQEQERIGLELAIGGMDMILASHMFIEKQKTDVEEFFEILLRDQMRIGYLEGMQLKEDSSLFYQRMDKIQDIIKEALNQTQQIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QUP4</accession>
    <name>A0A8S1QUP4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2380001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000247">
        <property type="protein sequence ID" value="CAD8118407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QUP4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="362" mass="42358" checksum="9AC00E4ACC9BEB1D" modified="2022-10-12" version="1">MIENEKDFCCYKGHKLPVVTIALDSKLSRNQRLLCTERLENADIDGKAVGLKKMIQLIEENQIKKMEKVETIITNQIKLIESLHSIFDQMKSYLMQQLEQLITIIIGWIQNLQQQRQQYSQFSFLEELEIMVMKSNQPDSDLYIHEIQKINLCWTSKLNPKLQQFNQFDAYNQCKQHLLSLELGSKKYTENDQQLQTNIKIMNQEISSLNTKTNIQPLQTDQFINYDLCRAIAIDKGCSTLLVGYESLIKLFEFNQGMIKKIQTLKQHKDWVQTLNFMQKFKSLYLGVVIIRQQYGIVNKIINGVLNKYQMDIIIVSNVQSQILMKIQQYQGVVITLQNFGQIRINGCANKQQRNTLNLYQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMA2</accession>
    <name>A0A8S1JMA2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMA2"/>
    <dbReference type="OMA" id="IDSCNYW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="114" mass="13698" checksum="9414C5CC9E5F48AE" modified="2022-10-12" version="1">MKVNNILILKNREDLYTRRQQAIFNKLAKEKKAIDAKQTKRVNKMLQISFMDIENFTNVSQSYEFNRHKDAKSLNSNRDHSISKSRYSNQVFLNKLDLTPRIDSCNYWYDLQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMV8</accession>
    <name>A0A8S1JMV8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMV8"/>
    <dbReference type="OMA" id="FQSGIHW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021836">
        <property type="entry name" value="DUF3429"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11911">
        <property type="entry name" value="DUF3429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="28415" checksum="30BDA5BA4ECBE1F6" modified="2022-10-12" version="1">MQKILRTLNPLYRFSTKKAQRIQNKIQDIKDQESTSTLESVAEQNTFTDEQLVKIIEDRRQFIRELTQETKAPKEVKYITIATLSPLILLSSATLYCCVNPAYAIYTGQLMHASIKYASINLAFQSGIHWGFAMSQHETQLDWLNSSSEARTTFFLASIPIIGAILISQYFLYGNDDNIQSISAIIGIVVMNLMVSGLDIRARSYAPGWFLKNKMFYSFGFHAALIPLVYCLFLYGDNFKNKVSIYRVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP40</accession>
    <name>A0A8S1JP40_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040372</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JP40"/>
    <dbReference type="OMA" id="EEHNQIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="201" mass="23946" checksum="B56542F8FDDCA819" modified="2022-10-12" version="1">MYSERKVKLTLHLPQLEQGVRFESEAQTTTSRSRIPSINKAQQTEKRLHKHTVTEISSPNQLHLYTPKQESLRAKRLKNIPYQLQCFIPNLPNKNFKSSHLMSNKLLEIPKSTLYNKDKWQLNMHKTRMNNAFSLGQSKRMENQHIKTEQQEEHNQIRRQNTNYIEKEKIKRLNKQRIDTQKLIDQFASQFNSSVKLDHLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP41</accession>
    <name>A0A8S1JP41_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein ARV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0050504</name>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0050506</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Mediator of sterol homeostasis involved in sterol uptake, trafficking and distribution into membranes.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Regulates also the sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ARV1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JP41"/>
    <dbReference type="OMA" id="WYFGCEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097036">
        <property type="term" value="P:regulation of plasma membrane sterol distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007290">
        <property type="entry name" value="Arv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467">
        <property type="entry name" value="ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467:SF0">
        <property type="entry name" value="PROTEIN ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04161">
        <property type="entry name" value="Arv1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8043849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8043851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="208" mass="24447" checksum="49356A969439BD69" modified="2022-10-12" version="1">MNCVNCNTKVENPNAMTQCPHCNHENQDWYFSCENNILLVDMILCKVSAYRHLLINSQYALIPASTILFITYILLELFYKYQSNILHKQEQELNFEDVQKQTIDITLTLLSQIVTIYIISLIIFRKLQHQQMIIGLILSQFGRLAIIMFVIWGHQPFYKQMASLSIIMSEWTTIRSLCEYKNEWLKSGIVIILTILLRYFLNNSITIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPZ6</accession>
    <name>A0A8S1JPZ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPZ6"/>
    <dbReference type="OMA" id="GGHYPLN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="208" mass="24452" checksum="CF98CB913CC26704" modified="2022-10-12" version="1">MKINSTGSEQRKNDQTLKNSKIEGLKLNQKNQTFKADEEIFKKKEKRNRKFFLKRSISLLQICKQSGQIEFKIGSDFLHIEIMQGGHYPLNQYNLKDIRSEIYVNDSEFQQSNITYSLYNASQSILKNCRNSQSEQNCKSLTQIQQQLKEINAFCQNIQKQNNKICQVDPQKQDSFLFKQNDQILQKIKETQQILLVQHQAIKNILQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JR23</accession>
    <name>A0A8S1JR23_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">NADH dehydrogenase subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0060141</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the complex I 75 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JR23"/>
    <dbReference type="OMA" id="QAMAYGV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.740:FF:000030">
        <property type="entry name" value="NADH-quinone oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.740:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.210:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054351">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010228">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015405">
        <property type="entry name" value="NDUFS1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01973">
        <property type="entry name" value="NuoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF13">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13510">
        <property type="entry name" value="Fer2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22151">
        <property type="entry name" value="Fer4_NDSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22117">
        <property type="entry name" value="Fer4_Nqo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09326">
        <property type="entry name" value="NADH_dhqG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00642">
        <property type="entry name" value="COMPLEX1_75K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00643">
        <property type="entry name" value="COMPLEX1_75K_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="11">
        <location>
            <begin position="25"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="13">
        <location>
            <begin position="103"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="12">
        <location>
            <begin position="240"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8044059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="710" mass="78624" checksum="66A29D82366868B1" modified="2022-10-12" version="1">MIRFSLQKGQQILRKQLSAFSTQQNLIEIFIDDVPHKVDPSATIFQACYSAGVIIPRFCYHERLAVAGNCRMCLVEVEKSPKPVAACAAQVAPGMRILTKSEKTRIARGGVMEFMLANHPLDCPICDQGGECDLQDISEQYGYGDSRHNEYKRAVEDKNFGPLVATSMNRCIHCTRCIRFSDEYAGVTELGTSGRGRNTEIGTYIDKMITSELQGNLADICPVGALTNGPYAFTSRPWELKSYDSVDVFDTILPLIQIDTRGAEIMRVLPRIHEEVNEEWLNDKSRQAFDGLKKQRLTLPLVRDAQGNFTDLYWSDAIQQAAKKLQSVKGEEIVGVIGEFADCESIVALKDLLNRFDSDNFEIRGNGVPQLDADFRTNYLLNSRITGVEEADVLLLVGTNPKVESPLLNSRILRATRKNNLKVFLIGPANDLTYNYVHLGNNASILDEIANGTHPFAARLKNAKLPMILTGAGVLERVDGNAIHNALKKIAQNSPVINPQLGWNGFNLLHKDVGRINALELGITARKSSVPAKVVILLGVDNNLKAQDIPKDAYVIYIGSHGDEGAYYADLILPGATYTEKNGTYVSTEGRVQTTKLVALPPSGAKSDWEILRALSEECGCALPYDTLEEVRYRMAELAPHLLKYDYIESSVLGQIALKPESSKQTISATPFKDLIDNFYMTDAISRQSVTMAKCSTAFNPHKFSTFKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JST8</accession>
    <name>A0A8S1JST8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ammonium transporter AmtB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0040409</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JST8"/>
    <dbReference type="OMA" id="VVWVLMD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000008">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF6">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="32"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8043186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="456" mass="49341" checksum="88AA7BCE158030CD" modified="2022-10-12" version="1">MGHLFRHQEGDAAAPAVANIPAPQASTIDYDWVLISGFIIVLTQVGFAFIEAGSVRYKNSQSIVIKVILGLFLTILIWWLFGYGFSFGYDFQTNFMGGTKLGGSNWEANQYGNDYTNFVFRASGAAIAVSVLSGAAAERMTFLAWSILAIVYAGFIYAGLAHWTLAKGWLTTLGYKDFSGAGVVFFAAGVAGLVVTVLLKPRRFRFDPNTTLQFQRHSPIYIAFGSILVFAGWLFYNGGIVAQGANSKYTQGLVAVNTLIAGATGGFFAFVIRFFQYETTSLVALSRGIISALVAVSAATDDMKPWTAFIYGLLAAVFYSVLAKVIPKVHIDDPVEVVPVFLGNGFLGIFLSAFFDTKAGVFYGFGAKLLGMQLLGLLIIFVWVAFFVLITLLVLKGFGVLRIDADTESVGIDKAHCLGEAIVFANQIDEVPLVQKTEMVQLGNSQIGSGFRPGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JU14</accession>
    <name>A0A8S1JU14_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0090114</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JU14"/>
    <dbReference type="OMA" id="PDYWTTE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="174"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="192"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="306"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="386"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8045089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="58436" checksum="2695F88DEE2863FA" modified="2022-10-12" version="1">MNIKVKFPNGHFLEFQCPDYWTTEQIQYFSAKNQSFYDPDTIKLYHGGVELYGNEKLLDIAKKQEQLVFLTMIGTKKQPSQTNKQLIYLEYTKNIQRTLVFNQLNLTNFQPIVSLMPIMGLKFQQNNQEKIQKPQQQRRQDDRDTPMQQEIFFHFKMICYIGAFYALFSSYFKGQNYYYLLVIIGIYYWIKYQEFKAQLRQQQQQQLQQQRQQQDQQRDINQEQQQQQQQQQQQQQQQQQQQQQQQQQQQDNNDNTIKEKHNFNLEQQQEKFDELDSFNEQDNELDLQLNSKEQLQEFLIRNAKLLQAQELQRDKQQNQIEKTEYEQKLNQQDKEIQNTINKEKKENQFQDNQEEIFKEELLFDERQFQENSQELPIKQNVQSNREIKIDKTSIDQQDKEQKENEDIDENIQEIQYEKQTKVEQDEEPELEEEIRKLKQLQQKQKRTFSSFFKAAFELIYCYIMSLFPFWHVGLYQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JUI9</accession>
    <name>A0A8S1JUI9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0060138</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JUI9"/>
    <dbReference type="OMA" id="HFAQGEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000118">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000121">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF149">
        <property type="entry name" value="GH01077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="9">
        <location>
            <begin position="81"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="10">
        <location>
            <begin position="232"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8044056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="516" mass="58107" checksum="CAAE2DEDDA9873CC" modified="2022-10-12" version="1">MFLRNRFHSLRKLAQFCTAESVSGKKTPAAQTQQTKVERNYQLQFSKSALVDFGELPTGEIPEALKYDRPQTLTQLETGLRVVSEQYNSPLASITVAVKAGSRFETLESSGVSNFISKLNLRGTTTKSREQVEAEIDYLGGSLKVKQGRELQTYTLTFLPSELERAVIFLGDILTNSLYSPAQIEAEREGIYRESVSINDQYRVVAEAAHYTNYRDHYMGQPAAGIRDNIPNITEEQIRQFHKANFVAPNVVVSAAGNVNHEDFVSAVNKAFKGLGTSVPTEVPNSEKPYATPSIMLMKDDELTNLNVGVFFDTPGWNHPDVFALHYFQRLIGDYRADKHTGFHLNAPSRQYNTMHSLLGGLPDVTYQRCVYYAYSDTGLFGNYLIGNEVFATQMAYVSQMVLSDYASSVGQVEVFRARAKVFNELLSQESSAKQSREIAQQVFYWGRRVPRSEFARRISALDAGHLTRVATRHFWDKDISVVVWGPTHLLDAVAHYNRSWKRSTLGGYAQPYYEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JW91</accession>
    <name>A0A8S1JW91_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0100382</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JW91"/>
    <dbReference type="OMA" id="MIRWYNS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF246">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE-LIKE 1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="294"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8046487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="423" mass="50331" checksum="830AE572D05B54E1" modified="2022-10-12" version="1">MTNITNLDKWVQKQNYTLIFFTRDDCNHCQRLEEELEKASEMINVGTPGSIGIPVAKIKNYELNRYPVLRLYVKGLYTEHTGEWEQKKLESWTDIRALSYISESDSEPTIRWIHESHNISVLVFNDSFKQELKWLKTLKHHIHFTYTTLPNARSILNVGEETTLVMFTEKGINRYDYDGEITYEKVFKFVEDHEEKYYQEFTQDAIETAFYEPKKPVLFIFDEKDYRDLAKTHQKEINTILVKQDQVTDRILNFLGIKGIQRPLAVIYDQNHSQKKYRSQFSELSELRKFVNNFLNNKLEPYYKSQTPVKNENWEKQILTIVGEDLPKHDNIFIYFYSSWCKVCQQFTPIFEQLFKKYRGQKAFGMFDASENEVKDQFIKEVPMIRWYNSQTRQVVTFDGPLTLDALSEFIDKQGKKQVSDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JY67</accession>
    <name>A0A8S1JY67_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF22">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035859934" evidence="1">
        <location>
            <begin position="18"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="174" mass="20505" checksum="B3F2455A6B0FC0B8" modified="2022-10-12" version="1">MKTTILLLLYIINQAYCQQNDEILGYMYVFAQEWPGSICKFQKCTKTYMGNYDNARWNTHGLWPNTMLQTSCGMIFNCKDEEYDEDKLTIATKTLIDVTWNGMYSDTFTFRKHEWEKHGTCHPDNLSQNGYMSRVGNLNNQYNYYKILASAGIYPDDDRXIVHLLQSIFYHLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0A4</accession>
    <name>A0A8S1K0A4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120349</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0A4"/>
    <dbReference type="OMA" id="EETQWVI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="526" mass="62314" checksum="35160F4898CE47FF" modified="2022-10-12" version="1">MSYQQKLNGLKCWDSFRDLTLQKMEIIPFAITECPDLCSCQICKKKQPEKEEPQEQNKKSQKIIKQPRQRRFSICGFKDDLLTICQMLQKQDEVVRPININILDTIILKEREFVLYAFYDNGFRYIKNKSFFHTEMEKQLLDRKIEFEINHQIKQIKSGIHPTIKLSELYQNSNLVTNKLKQQITDILNAQSILMARYQTGETTLIGKIELHQLSVLSKTNVIFDNLLHLQLYLYNEKTALDFIETNKRNSKDYFIHRYKYIKDNSLFSDIVCEQSYLTDPSLRGQRLWNKEKEYSENLDLKEYFKYTCHKIAVYIQSFHKQCINEMEVHFIVNYPKNITIINVIGLKMSKAEETQWVIKTGRVLSTAEKIQINDVDLNENNIPQTQRSLVVSQSLLPSTQKVIEKIKPITAQVIVNNMQSTRTQDAFKTLNPQTNKSINQIQENQQYQKMLRNFPKQLKRYQSMQRDSPIKSRIDDQLLTCSRDVQIDDYLLKSRQKKKKQTINSPFLRSISRRFFTRRNSADFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1Z1</accession>
    <name>A0A8S1K1Z1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0130111</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1Z1"/>
    <dbReference type="OMA" id="QPINDVH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="342"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="433"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="574"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8048862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="608" mass="71558" checksum="E5D4CA7AF8A62BAA" modified="2022-10-12" version="1">MQSRTSQSAKQIEADTPAQNTSDDGKLLKSVRKVQQIRQLLTVNTPAKSLPSYQLDAAPLKEYNPQKQLSKQITFDEVNDEELQRYKAEWHWDVFFEFLFYHMLFFILLGPLIVLVLFKYPGIILLKNMRFCKHSSAFYMQTLLWMGSAIGGLMYFIQDQSVITLTEIIFMWFAISTRSVVIAAKYATLSTARIKLYKSEELPDEMFNFDLMFQDWFEQSSKVLFLEQYRALRRHEQEYSLYKFDFLLDPNKKTADAIKTTNICFYVEWAMKQQMKQTDPIIEQVYYNGFHVFGYLVNYFQKTNPTSSFFKYMFALGLFYGISPTFIRLQHFMENIPDWLDIIRLIINFLTSMQAFFGSVIFLAIGIYDFRRKFFLLDQCLYMLSAKKVTYPTAKKLIPTINFNNPQTLQAWSIIRSLCFDYGQTYNLRVQGFYSLIFIANIVFIFISLGLILDLIHIDMFQLILLGEMAMILIGFTLYYLYLGASLNEYFDSFETLLDDVKSIYCDILRMREQYFQNNIQPINDVHKIFVNIIKDQVGFDVDTVKEYIELIVEELENNQRQLTYDKRNNPFKLYGVVITFNLLKSAGVGLSTVFSYAIQQRLSTKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7L0</accession>
    <name>A0A8S1K7L0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0100383</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM208 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7L0"/>
    <dbReference type="OMA" id="STRLQQW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008506">
        <property type="entry name" value="SND2/TMEM208"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13505">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13505:SF7">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05620">
        <property type="entry name" value="TMEM208_SND2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="128"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8046488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="149" mass="17332" checksum="9020FD0A7A4805C6" modified="2022-10-12" version="1">MGNQSEKKIANFKQKVIGYYFYPTTSICILFLLANLYYGYALTIWGGIVISIITLIQYFCYNQISKSLDLGLNADSYSIYLDILALNGAIQLGSTLFDFFWYGYWIIPLILLYKGSQIIWGWLQKPQYAEDDKKDKKKKDNQPKVKYVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDI6</accession>
    <name>A0A8S1KDI6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0190336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDI6"/>
    <dbReference type="OMA" id="FDYINLI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="60620" checksum="0D12C7917E1A263F" modified="2022-10-12" version="1">MNSFMNTITKLDGFFVSFSPSFGLGEQKVFYKSVWGGMATIIILTFVFVYSINQFILWGSGDMIYKISSQSKTINVGSHLESLFQKNDPIQYLDIQFESSINPFDNNEMIILPLLYANIEETLYLLEYQDDGFQSLYLELNNQLFNDFNIILVKCIGNEEYLNENQKCASQEYSDQFFNQIGLLSIVTIYNEEFDFSTKTYSDTETYVDLPFDTILSSEVRIFTNYEVIEINQGFLFQQSEQYIVNRGSSAQIYTYTKDYHKKLYERDLGVLEVIGSVYLQMNPTIFYVRNQYPTISEVLANIGSIIQTILLIRYMFYALNRKQLNSYIQSQILSNYYPEWKDIKKIKKNKISSCVLDDKKINKTSIQKFEKEVTEILNLKFDYINLIYEIHQMQKILQLITPSDLLLNIHSNQSKLQFQADQDSNEFSIQNAWSSYDDYSNTIKQNNLDPFLFSYYWKTKMKDNDIDKISSPENEQLQKQCKPTQIKENVDLVVDELRVQDSKIRLENNEQMIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEW2</accession>
    <name>A0A8S1KEW2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEW2"/>
    <dbReference type="OMA" id="TTHRYND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="314" mass="37106" checksum="0204B3A69DF13C0A" modified="2022-10-12" version="1">MYKGYPTEVKSSAGQRITRPYSQQKEYIDMKSSLKQQSQSCEKSNQSTILKGKVSNKQQVKNKMKPTQTRFQFQQPQSLQTTHRYNDSPYVKPHQQQQLFELQKTIMADSNRVGNIVNQMKFHSQTKKNPQQPQRQHKSNQTNCQEKQSQSLNGSFELKKRIFTTNQDVTSKLNMITKSNRAQSVMDEYKTTAIELQQTMKIPEPILPQRIIPYDSETEFEGYRKGDFLTFESEDESENEDFLRKAGIEHFLKNLTDKEILLLEKYIYLRKYDMMARNPKTSKRNEQFLNNNNTSRTSQQYQQQFCKISRQTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KFV6</accession>
    <name>A0A8S1KFV6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0190306</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KFV6"/>
    <dbReference type="OMA" id="ENECFHC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000032">
        <property type="entry name" value="Glycine-rich RNA-binding protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000950">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051186">
        <property type="entry name" value="RRM_HNRPC/RALY_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968:SF26">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="92"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="105"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="142"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8052685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="191" mass="22338" checksum="C3337498F6B6B5B7" modified="2022-10-12" version="1">MGDIIPNQLFVAGYSRNKVQDEKDVRDIFKKYGTVKEVAYKGSYSFVTFNNEQEAQEALKGTNGQTYNGQKLKVDVVDNRKGRKTGPNEEDKCFKCNKGGHWARNCPNIKSPRRSRRRSDSRSKRRHRRSESRSYSSYSSSRSRRRRYQNKRKRHSRSPRRDQRIRKRSVTPKRSPSDSASSKRPNSNSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHP0</accession>
    <name>A0A8S1KHP0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHP0"/>
    <dbReference type="OMA" id="HMGNQDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="169"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="406" mass="48800" checksum="B86DBBCF38D8EDB0" modified="2022-10-12" version="1">MSSKDKDKKKKTAKLEKKDTKILSIPDLDQSQLIARVISRGGDKETKNIANNEDEMQTFTQSHSNGVAQMNDFQGIQSQQQFNTNFLRNNDYNTNKFMSDSTYEEYRGQLNNSLYYQQQTLSMQNYAQQYPQQYYHMGNQDQIDYRYEKIRNQINLSLLPKRQEILYQIQKIENRIDEIKYQTQQIENMTREECDFIIDRLRSAENQKLQRLYQDKDELISNIEQIDSLQSYALNNQEQIPQSKILENIERIANQRIKTQIDIYSNDLPQEFIQLKQSYQQNIIQQQLIEFKNEVIWKLVNDSKIQIQKIQADLEQQVYQEYQKWLNNEEKYKQELNKYKLKCTFCGVNFDYKQINTNCQSNIQNAKLQFECEEIPQEQYIGTYRHFFSKSKELQESQMQIQNKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KMM8</accession>
    <name>A0A8S1KMM8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220369</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8055025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KMM8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="94" mass="11033" checksum="D5591A7202AE629E" modified="2022-10-12" version="1">MHGYNDQALNFQYSSFLVELILIFIHFDFQAAGSLKKKTLQFMEFCYFQSFKGGVQSSFSDWIFQKDRLMGTMTASMRTLMNENIKFQVLNSPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRT2</accession>
    <name>A0A8S1KRT2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0270069</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0270070</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRT2"/>
    <dbReference type="OMA" id="ITIHLRT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8058151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="28160" checksum="568D73B16BB19E6E" modified="2022-10-12" version="1">MLHQLAQLIDDNREEKENLGSKHVRFLSEKDVDQTSQNTQAITSEQNQDDNDSFPVIILILCLFLLICVCGTIVEVSRRKKVKKEIEQKLLQEKRLSLQANIGMLNSIQDQMQFTLWKLTSFNYKNFSTQKLLRFQSFFTSHNKLELQIRNEQDHDPWSAEGHISINQEQEINIHLRTSLDCELLEKNEKCDHLYLLQQFKGVYNKEENKFIGSWSLYGSQELNNLNFFGEFELMRVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KS13</accession>
    <name>A0A8S1KS13_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nephrocystin-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0210061</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CKFNIEA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058686">
        <property type="entry name" value="Ig_NPHP4_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029775">
        <property type="entry name" value="NPHP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058765">
        <property type="entry name" value="NPHP4_C2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31043">
        <property type="entry name" value="NEPHROCYSTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31043:SF3">
        <property type="entry name" value="NEPHROCYSTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26015">
        <property type="entry name" value="Ig_NPH4_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26186">
        <property type="entry name" value="NPHP4_C2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NPHP4 C2-like" evidence="3">
        <location>
            <begin position="700"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="NPHP4 Ig-like" evidence="2">
        <location>
            <begin position="1136"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8053784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1315" mass="152631" checksum="70E6C84898F37D73" modified="2022-10-12" version="1">MSTEDINSIKKFLKFQKMHKSLPSSIPIQMGLDSKAYKLIVSRIGGLRRPQNPVLNLQFSVAANFFIQNQQGDKCFFGRTYNSPRLNVEFGPDGVNYEHPIIPIYYHTRILNNKEMPLLIILEIQSYETQQDLVLKRMHLGWCELQALTITTQSTKLNFRKGSPRILLLGQQVNFESSGISVTCDLVQSEEFQISMHLSPENALCGLGDELPGLKKSFVGGDFELQRSMVVFMSKIQVLIPDTMEADIYRQVQKYRTYKYRTPDDYKGDIQIINKRLIVAFHNGWTFINTRGTNNFVPLGETRMLVNEFVDQKQIQYKQLVYHGVIDINNVCSTDPTVNSGMLVAQLEYEAMFLVSNLTKETMQLTLGWIPIYINNKFGADEVLEEDFIIGPGKTLDGRPLVSFQNPDLSIKLKGSLNLSGGGFQIQDLYMGAAQQQQVPQQQGYQQGPLQQGLQQQSNLNQNQSLQQQYQNKQEQQVRGSDIDIRTQVKLKDFEQQLLRLQEENERLRRLAIQQTADPQKQQEYLQAIEKLREENRLLQFTQAKQFEGLEKLIKDQKTVDRTAAPPVVLAEAKPQNTVIKPIDSYAYPVPSANLKPITRTDVTALVDDPISIDQENRLMALEMQDERKDQIIVIQPVSMKQILGPYPICKSIQIGIIFYDLQTFKSPFLNLEYYNDERNFKYNLENGNPLRLLYNNEVFSCKFNIETSLQRYANLEQDFARYLFNKNLILDIWDSESLFLFGSIRIPLRGIMRGGKQNAQIMADVEIFDPQSKKVKGTLQIVIKNIGKIGQQDMSKTVPLENTQNGFQKKKLVSEKPVNRMDVPAQAFDGNSIQDRIKKIKQSNLQEMIKNKQSQQRSLNEVLKYKEIKKPSLVRTIMQGFLQNEKVVHSFYGKCEIVPLYFKNTHPKPEQFTVNIEDPESRMLEYQEFFLIGNPAEWKYWVTNNKYPEPENYDLIRMKENQGKTQYYFILDPNQEVTLLFKFISFRQYDYRVDDHERTPEYIKKYMQSRHITILLQQDGKMMDGLRIIVEPHQQPIDHVLRFYERENRQTRLVIPRLFNQPLQQQPHVHITDPDVMIEWSSDFNSIRLNLRIGEPQSVKIFNILLYQDQYQHKIIANWKIEVYSLSTIDIEVPLGQRVCTKITFPCDITRIIKMTSSNKMVSFVKPFDSEITMLPGKINLVPINILALQIGKHIIKLNGVDIHSQELIYSWLVNVHAQPQPPSATYDLRCIDGRESRHQVNYKNQVNYTTQYNIVSSSEFVKIIDHSISTGPNEIVQFKIRVLPGSEELVHIFITDIDERIFDVIVLNIKYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSL2</accession>
    <name>A0A8S1KSL2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YINYHEG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1285" mass="153500" checksum="01A4D48B7F9F271F" modified="2022-10-12" version="1">MQFHYHKQEIKITFKKSIQQYKGLLFKNINPKLDIYRDKSISFRNGKVIGKTWIQDITCFDEEIEDCSFHKKGCHIVLRNSLDIIRVISENFITLRIRKNATHRIQLNEENYINYHEGTLNGQPNKLITQNSYYIEEMNCAINQNQLIPIGIKIEPLIKYSKIRCIKISEETYCIQQINKEQCLNILIHQDKQVPFYNDVLYLEGQLSYINGYFLLYNNEKLQMINQNGIEYEETLHGQCYLDGSEIFILRDISIDEQFVKNTIDFTIKVTGVLLKIQIITKSLKLKMMLEENQIPPQEIPDYLVKINDQKFIKETVKKLWKQKSINKNQLIEIYNVLVGMKKSNKLRGKLNLIQCYETENLDNLSFLNCISSETCDLQQIIKLFQNHIIYNYIHFLNLIIKHKPQFLDEVLPNGTLFQNFEPLELGTLSKQNKLNDLSFLGTYNVPKIQTNYQKIASQFLIQCHGIYYLPIQQEDFTFFLYTLLIKGGQSRLTLKQFMQEQDKLHTLFTYSSKIESLFGQLNQYYQEHNLNWFDEMLNQLRKTQYPVQIFQSILDYCPNLTTKEYLVPVNLAFERLLTFSTDNYQLFQQLLDSFIFIRNSFDQDEYDQLQNLISLLQQSTILSQIISNFNVIYQLNTIINKIMQNPEESQKCIEQLLENYHVNRVKQMQFKIQDKIFNLQNQNELKQCFSFGFKNYIKDEKKIELSIFKKNLEFGKYRDVKLLCQTFNLSINQFDEAISDAMYRYLQLQKKIDYIEDERKKFLEIVNEIQKIKKEKLVQTKKTMSLYKFIIESKEVGNYKDLDLFKCNQILFQIYRRTQNLDLPYLRLKKKLAKANNLAIYDREFCKFMIDLAQKNFNIKPQKAQLILDDLSKNGDEIIQSEVSIILLQFSKDENQKKEQYWKVLKQSSSADTVQTLLKFQQKQSKQIQLMPQKIEIISKELIEVSLDNSISIAKFEQEYKKFYSENIIDVVFLDTLLKDPQEAFFLLSIKQSQNTKLLIQKLVQSQLLNSEKILNYYIQTLVKPFNVINLLQGDDKELEELAQALVISQHKQIFDKRCIFNKNERLNQIMERLDTLELDNIIPELLYFKDLNIKELKIQNFIKRIQQTNLRQYYKNQEQSLTSYVKNQIKHNPEIINIQIDLRYHFIIYEDIYGFTIKEKYLIRSFIYQCIESNKSIQNYDNIYKQIISKEFNFEQVKEDFYQIIELECLFIISEIAKYYYQKQEEVIQFYCQKLLQNSQCNDEITFWQKQDPQNQIRNILIDTLKKQNQSLQLNNELRSLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KST6</accession>
    <name>A0A8S1KST6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0270231</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KST6"/>
    <dbReference type="OMA" id="CLIICDK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="127"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="439"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="445"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8058459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="468" mass="54981" checksum="5ED7048661EC47D3" modified="2022-10-12" version="1">MSQDKKFYFDFTSPFEMERQIEIFPKSLKEQFFIPKNDKFKMKIIVLQRNYLIIQNEKVSYKTNIQHTFQKYFNITNAFLEIVSHPKMGLGVSLTKNGVNFRFYGLVQQWFVYMKKWCIQSYFSNEYSIISLIGKGAFAKVFKIQRISDRKELAVKVFDKTILQCKDKLALLKEIELLRLMNHKNVVSIQEIYENDYYIYLVQELYQGGELHMEVMKNKTFTERKAFIIISQVVEALHYVHFHGILHRDIKPENIILREEGIIDQVILADFGLADYYRKDCKYMFTRCGTPGYVAPELLQDKIYDHKVDVYSCGILFYYLLVGKGPFDSNNYDTTVKANQNGHVDLTQFSFTNECLDLLRGMLDPNPQNRFTLDQVNLSPFFKKHTFANNPINHSISLESLKNKSHSQTHSLQLSPYQEQRYKQELNSRFAQYASPLQSPLRLKSPNSSPLTDSQHQSPNLQRVPIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSY2</accession>
    <name>A0A8S1KSY2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0270251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSY2"/>
    <dbReference type="OMA" id="AIQYICQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039273">
        <property type="entry name" value="TEPSIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514:SF0">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="491"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="511" mass="59530" checksum="EFBE0D9CC7E1C19F" modified="2022-10-12" version="1">MSSILNKFKKQTTNDTPNGIKFENTSFGQQAGTYQPPIIPAVQLTQSNQPRQKGRPGGGWDDEPQTQPEQTSISTNKATNSINLLNNTPPKQNNDQKSNHEVLLPPQPVIGSVQLSRITDYRKGQLLTYEELELGGFDYEFHLVDEVIKLGGIKLKQSDNVLKDFSRSIATIQDQLIGSILLNKLHSEDNWKVQIRCIYAIQYICQQFQNYREFFQINQQYLKVDTDQQLLSGAIDQTLAEVNGQKVTKQKEFEFEFREPPKIQQPLQSQQPNLIDILDVTSNDPQQQKQQQQKLNLKQLKIKQPTAQIQQQPIVQQQPLVQQQQVQQKNTQQINLLEAFSDISLENYNQQQFQQTQQQSYQYQQQPQIQQQQQNQFQYQQQQQQQQQQQQQQQQIQNGISFDDLLNSQTTNQQQTQPKKNAFGFLKKDQQQTTIQNNQQTQQPINLLNSPPQIYQQPQINNQVPQQQAQFIYNQSQSNQNQQMYLQPQTQFNNQQQSQNNKKDLIDFLNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTG4</accession>
    <name>A0A8S1KTG4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0240084</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTG4"/>
    <dbReference type="OMA" id="YAYTGLK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="446"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="262"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8055986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="519" mass="59938" checksum="E0C61C91D80DCDA3" modified="2022-10-12" version="1">MQLETELTEYDVSPNQSQNSPKKEYKQKPTKIGEKCKAILMVVFYLGTLFTFNGENIFFIGIYMLMLINNRKHLFDLVLTILAITLSIANLFVEYNLLGISQIIMSFDILTFSFSQRMIREIKLVWSEIKNLAITMFFVLCLCAYSTTAYYDDSEDDEIEIFYGTFGRSVLTFVQALSLDDWAQIGRESDDLFGYFILCIYILLMTYFYLNIMMGILIETLQFEKKGNHLDDPFNYEINQQQILSELVKDQNLFDKCIIGNYYEYIVFIISFGAIFISILEHFDHGDSTIELIIELIDDGLYTIHFIILILKKGPIYDLERKNKTVMFLHFIAGPLSLICSFIFLEIGCLLNLLKIATTPSVKGIFVGTVAMVPLLMPQFAELFGIIMFIASILTSNYYAYTGLKQQEYFSSFWGSFYTLVQIMTLDDWGNIVEPMYSKHGFILPYILIPAYIFLSNFIILNTLIALSCEYFVEVKYYSIEKDQNDVRSSQFVGINELKNYLSSYLSALQSPQCGRQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTT7</accession>
    <name>A0A8S1KTT7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0270135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTT7"/>
    <dbReference type="OMA" id="NEDDHFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="491"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8058277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="911" mass="108873" checksum="D34C14669D27D060" modified="2022-10-12" version="1">MHSLNSVHLDNINTVGQQDTSICKNIPVNNECDSSSIILLKKIEAPSPDIPGFNQAVFKPFQKLIIYYHIGFFVRKLLKYIKPDQTFKPKHYQLINDKSSGNIKDFITYAGKSKSTVMQYPSLEEVNFFVRMRYRYRQGLVNFQQKILYLFEQIPLIDPSYKIKIIWDLLLNIFRIYLMYIIPIIITFEQLIEDYDYIIIISQIVFAIDLLLRNITIYYDQGLPVVDRYQIVKNQYHVTNLIELMEICLLFALAYFKFDFNHQFILFEGWPKLWMLLYFIQLKNLLNFIGSWQQSFLMGQLASSLIELFKLIGLLLIIQHIFSCIWLIIGKYHLLLGNSNWITFFNLDHISWTDLYIESMYYTSVTMYTVGYGDLHPINISEKMFAFLFVFVCTFQLSFSLNTIGEILTRMKNNNDIINKKLIFINQYMHNKKISHQLQFQVREYLNYYWYQEQTQQTKEQTEILSQLSDDLRKQIAVESNSIVLKNCDFFYKNFSTEFLNELLKNLQFQAYQPSSTINIFNEDDHFIHIIESGQVDIYDKSFDKKVLIGSFKSNELFGLNEFLNNQKNYKYEYKSSGFVSTLVIPRSKFHMILSKYKQDFETFWNLKINNYSELKCAVCESKRHPPDLCKQVHYIPDKEKVIKQFNFYQNQDRQKFKRNSIRIKQLYHASTDQEIIKEAAQMVKVKNDSIFFQKYQIIEDETTQYESQEYINKKDIDSVGILIKDLESRSYLDLYQKSQQKSEKEILDIRIHFSLELQTFKSLKDKLHHKDLYTDKDIQQIEYLIKILELKLNLEDKIELDQQQEYQTFNQKANINTVIKKLIHPVRYIITSTSKSLDQFQLISGNGWLKYMFYPYDFINQYRFNVNRLGLPRKSMVVRQQERRLSSALMRKKQQNIRSMKRSIQVHPIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KU96</accession>
    <name>A0A8S1KU96_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0250281</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KU96"/>
    <dbReference type="OMA" id="VQPQHNP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="350"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8057133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="645" mass="75751" checksum="8044FCD565575F09" modified="2022-10-12" version="1">MGNSDSNASIKALHTNYMVIRNIKDDPRYGEGRIVQQKQTKQEAFQKEINLTDEDKFKKFKVLLEKKESNKHENLINVTKLYFHEESQFCGQFFQIYILLEYIPQSLKETIEKRLTNKQYFKEKDLMQILKGCIDALYILQFQNITHQAIRPETISYQEQQQLVKLSDPTVSGLLSSFQLVVQEELKQINQYYLSPQLMQSLNDEVQPQHNPYKSDVYSLGMTMLHLSTLNNCDDCYDIPKLKVLLQQVQRRLQQIETTFSSQYVSLLRKMLLLNEDQRPDFIQLKYELQASLSSQSQLYEEQQIIQIQKAPIIQVQNYEQIIETSPIQLQSMQMVMNSQERNNLKIQQSNQSIRNSQIQKSQELRQSQQSNQSNYIDRNEVKELRPDISDHIKILPLSEQIAEPSGIDDNIGNQIGIDKFKHITNEPEQQNILMPVTSFETSQQYEQFIKMQQSKSNYYITDQSQKVDQNMRLINSFNTKRSDIEKVIEIYPNGSKYDGEKQNGLRQGYGIFYFQDNGGVYEGQWYDNKMHGNGTLFYASGKPAYEGQWVNDKFQGKGTLYNEEPQMLYSEFDYRDFDQVGEYWTKYIGQFYDDNKEGQGILYLSNGDRFDGTFIQDLISGLGKYIKVNGQMISGRWWRNKLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KX51</accession>
    <name>A0A8S1KX51_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0270234</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KX51"/>
    <dbReference type="OMA" id="TCFAYIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF106">
        <property type="entry name" value="NIMA-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="228"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8058465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="625" mass="74968" checksum="CBCF34255E9A0420" modified="2022-10-12" version="1">MDFYYNYSIIEQRYANQFGIQNNVSRIEANFSQQNYTQLVSIGIPKYNIDERNLYRYRILKYLNVLQPEQYCCTQDEHFLIYQNLYQHLLNEQKLKNIIQQEGFKILLQLVLIFAEFQRRKFYWPLNSINQIYFIDGIIYISLLEYDFTKFNQPLNYLDNFWKFFTQYFIHEYQFQEMAYLMNQQNFDQILEYLFIKNGNIHQQSGLNPKYEKVLQYLLKIDKFNSLYQGNCVVKEFKKNEVFSIYPNLTNDIVYKSTEIKGNEDSQKTILQQIQRDIELMELFSYNENVATCFAYIRIQQYAFLLFRKFFGTLANRFETWPKRDQTEEQIRKDVYVITSRTADSLKILHEKQVIHRDLKPENVFIDNENLSDSFSYIADFDCSKQIQGQINVTLTNVDSSYTQNYDPPETYQSFKYDIFQLGLIFLTIANKGKYIGDYKRGPRLLQDHEFVKYYSLEAIQRSLQSTNYDINFINIIAQCLHRDPNRRPDIGTICNLLGQFKKNVQIKIIRKSIIQQQDQTNEQQQSIQTNDQNQKSNHQIQRSNTITIMQNSTNNQAFQEQSLTPTNYQQQQKIQINYQNPVDLNSPQQINNLQQSQFQQLQQSQSTRNLYQTSNNKIKFWLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZB3</accession>
    <name>A0A8S1KZB3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0280015</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZB3"/>
    <dbReference type="OMA" id="NTNNDRW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000557">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001606">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="113"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="410"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="448"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="484"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="523"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8058782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="552" mass="63324" checksum="4D040A7118AA23FD" modified="2022-10-12" version="1">MGCCTFKLYKEEAVLTIEDRQQDQIDAGVTHQIVGNVTTKILFQDKETSAIKSSSLSKEQIYQKDLEQQQNQTKNKEGNNFEMGRKSISGTMKLTPEMLVRKQCITEKFLSHYEIVKKLGQGGFGEVYLVKHLSTDNLRAVKVILRKTINCEEKLLEETEILKTLDHPNIVKVLEIFADFKYYYIVTEYCKGGELLDRIKTISKYNERLTAKYMKQVFSAILYCHQKNIVHRDLKPENILFDSNDPDANLKVIDFGASEKMINQSCLTKKVGTPYYVAPEVISEPGYDLKVDVWSCGVILYILMIGRPPFKGSNDIETLRLAKQGKLNTNNDRWNKLSDQVKDLINKMIVVDPKQRITMEQAFNHIWIQNNENQDIEDFNIIQTLSHFSAQNKLRAAIIQFISVQLVNKEESTKLFQTFKSLDLNGDGVLSKEELLKGILTADIDHFQAEIMVNDLMQELDVNESGKVDFTEFISAALVLQSKITINNIKNAFKMFDLDGNGVISKNELENIFGGIEIDNQAWDDILEKFDYNKDGVIEEEEFIKLLENIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L157</accession>
    <name>A0A8S1L157_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0300242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L157"/>
    <dbReference type="OMA" id="MSMLFTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03710">
        <property type="entry name" value="BipA_TypA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01891">
        <property type="entry name" value="TypA_BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000002">
        <property type="entry name" value="GTP-binding protein TypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000055">
        <property type="entry name" value="GTP-binding protein TypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048876">
        <property type="entry name" value="BipA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047041">
        <property type="entry name" value="BipA_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035651">
        <property type="entry name" value="BipA_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF8">
        <property type="entry name" value="TR-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21018">
        <property type="entry name" value="BipA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="1">
        <location>
            <begin position="42"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="646" mass="71900" checksum="AB373AA77B25436F" modified="2022-10-12" version="1">MLRTLRQFSSIFHRVPLRSFSGAVEVTPKDDILKILHSESRDIFRNVAIIAHVDHGKTTLVDALLRASGCANEYDSMDSNALEKEKGITILSKVTGVTFGGNKINIVDTPGHQDFGGEVERIMSMVDGVCLLVCATEGPMAQTRFVLQKALQSNLKPIVIINKVDRPSARPVEVEHEIFNLFCDLEAPSESLDYPLYFCSGKEGWVRKGSMEAEKQGLEDVLETIVETIPPPQIGTESSFKMLVSQQESHPYYGKIVIGKIHQGEIKLNDKILAVDQQGKLVETAKVLKILRRYGMQQLEMARAVAGDIVQVAGFTNAIVTNTLNEMGKNDVIPSIPIDPPMISVSIGVNTGPLAGKEGTKLNAQQIKERLMREGQSDVALQVMVKDKSDDSACVQLLGRGDLHLAILLENMRREGFEMQVSPPQIVTKKCPDTGKTLEPMEKVTIEIDQQYMTGLIDKMSQRKAIYEDCINIDKTRVKLIFSAPTRGLVGLRAELINDTRGTAIMQQQFLGYQEYRGTLKKNLKGAIISMAAGVCQGYALEDIQKFGPLFVKPGSKVYVGQVIGEHKLEQDIEVNPIREKKLTNVRTVLADEKISLFPPKVFTLEDVIAYIRDDELVEVTPKDLRIRKKELDSTLRKTQRKNQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1Y5</accession>
    <name>A0A8S1L1Y5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-related protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0310094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1Y5"/>
    <dbReference type="OMA" id="GIHYPIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10221">
        <property type="entry name" value="ASKHA_NBD_Arp3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000029">
        <property type="entry name" value="Actin-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="49006" checksum="8C245E016D0E00AC" modified="2022-10-12" version="1">MQQTIDQGFYQSKVCVIDNGTGFTKMGWAGNTEPQFDIPTVVADHQEKQKVQMSKNTNENLDYYIGYEAYDYTKSHNIFYPMKSGIVENWDLMEKFWHRSIFDYLRAEPDETTFILTEPPMNPPENRENIAEIFFETFNAKGLHISVQAVLSLFSSAFAPVNEYQKTAGLTGMVLDSGDGVTHCIPVSDGYVIGSCIKHIPLAGRDITQFIQTLLKDRGENIPAEDIKKVAREVKEKYGYCISDGDLIKEFQAYDRGEKNKFKTYYGKNSVTKEKYTIDIGYERFLGPEMFFHPEFLDGKWRSPIDEIIDKSIQTSPVDCRKKLYQNIILSGGSTLFDGFAQRLEQQVQLRIDSRLQKYEQRSGFKPAPIKVNVTQNPFQRYAVWQGGSLMALQPQFEKVYHTRQEYFERGPSIARYNAVFQPQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3L6</accession>
    <name>A0A8S1L3L6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lissencephaly-1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0320312</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <text evidence="3">Localizes to the plus end of microtubules and to the centrosome.</text>
    </comment>
    <comment type="domain">
        <text evidence="3">Dimerization mediated by the LisH domain may be required to activate dynein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat LIS1/nudF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8062067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L3L6"/>
    <dbReference type="OMA" id="WHVATKE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051012">
        <property type="term" value="P:microtubule sliding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03141">
        <property type="entry name" value="lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017252">
        <property type="entry name" value="Dynein_regulator_LIS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="101"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="143"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="185"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="227"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="328"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="53"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8062067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="403" mass="46188" checksum="D997CA8823689D41" modified="2022-10-12" version="1">MVLTARQRDELNQAIHQYLLISYQQSAQVFKVEAVVKDGQIEADLLEKKWNSIVRLSKRVITLEQQVEQLNEQLAQAQAGKIQFNKSDDEQRLTPIEKFKLEGHRAGVNCIAFHPQYQILGSASDDGSIKLWDYESGHFEKTLKGHTSNVNCLAFDPTGKYICSASSDLSIKLWELKNHTCVKTLIGHEHSVSTVQFSDHGDFILSASRDKSIKLWEVQTGFCKKTFSEHQEWVRCAVFSNDEKQMASCSQDQMIYIWVIDNGQILHQLSGHEHVVEQVKYVPEHGAKQILTQQQQQNIQTINLLVSVSRDKEIKIWNTILGTNLFTLSGHDNWVNGVSFHPDGIHMLSVSDDKTIRVWNLKEQKQKKKIENAHDKFILKCEINKFIFATCSVDQTIKLWLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6W5</accession>
    <name>A0A8S1L6W5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0350070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6W5"/>
    <dbReference type="OMA" id="ICQQWSK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="184"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="450" mass="52672" checksum="15EA5BB21CB8F5D5" modified="2022-10-12" version="1">MKQNLPQQSSIIFELCDQFQTQLLNFKNKIKTQLQHLDDLINSIIELVQEKINQNIIEENDIIEDVFLCCQAFIENKLDDCLAMVKNLIIQIQISIQRSKQQNNKQGTLINSNQYEYMDFYDQSTQCSSSNINKYELKQTIKQKEICFSLIFNSQNSIMISGCGENIKQWKIINGLIENDPLILYGHTKLVTCLIYTNDDRCFISGSDDGDIRLWKKVQPNQWQSTTLTVHRQGVIGVLFNSQNQQIISFGKDPLINIIKFGQQNLMQIKQTLNKHQSILNCLCMNESESILASSDSQKNIMIWVKDNNAQWQFKQIVNQSTNDYFCRMVFINNYLICQQWSKPISQVFVEVNGQFNQRPDLQIQLSEQNQDDEDLFPTIYNKKKGILIQKYGQYVYILKQLNNCQFSQICPPINCIDQYNYGNLNKDGNILIIWNYKTMEFRNYDLSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L975</accession>
    <name>A0A8S1L975_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">VASt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0360262</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NCIAIKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47666">
        <property type="entry name" value="PROTEIN VASCULAR ASSOCIATED DEATH 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47666:SF1">
        <property type="entry name" value="PROTEIN VASCULAR ASSOCIATED DEATH 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1556"/>
            <end position="1573"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="5">
        <location>
            <begin position="1277"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1117"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1476"/>
            <end position="1556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8064710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1574" mass="184354" checksum="8313372291932A95" modified="2022-10-12" version="1">MLNASEQAATSNLNDKLEDLKKIMKQYSKYTSDSQNEFETHFKKYGPFNKQYSQQISLSVNDPISLGHNLMEFIQLFELISKTTLSQFSEFQQFFSQSIEQYNEKMNIFTKIITDGNFQQSQQLAQHRQNCKKTKDKYDKLCKEIEMTLSSSKKNSEDAFTGYDLNLSQKNDQKLKELVKQVEPAEQQYKDAFDSLTIKTKEFNQAIDDSRQQLIQAHQLTYQRFVEITMKLDQHKLSANCWIKGQMDEKQEKIITMPKYEQEMEDRIITSKYEQDNQFRYINIMNKVIEKKPITDQEYLIFPDVLHKHIDAQLSFINDRVKNQKTLPNYLLEISNQMDAVSKNLQKSIKTTLQFIQDKSDKDKSNFIKPILQVQELLVKQNDQYSKKYNQQSQFIQLKAQCLDQLIKEQKNQEKSFLNMYQKMLKDFQGLKQQILENEQKPQIKENLLQLLKKQIQDNCIAIKYSVEQVRTNEFQRSNQIIQTLEQILGHYSSFIDEIITYTKQQEENLSQIQEKIAQIEYGTMINTKLDQPEMKFQKLFEMTDEIKSKIETATLLQQQEDSESDDINLEEGSVKVSQKLIEKFRILDGDKCIASYACAFDNKILLQGRMYIFSSKVCFHSYFNGKTLFGTTALGIPSIDIQSIRRIKAYMVDAALEFKTLKGTLVFASLANRDQTLGSLKQVKGLESGIIEERIESDPERNIQQVYVETQQLVKPQEKQQGIRSPSPKPQNIQAETINLKNIESSNDKLQQIDQSPKILNFQQQQQSKNYQLSKMINIPKTQCLDQLIKEQKNQEKSFLNMYQKMLKDFQGLKQQILENEQKPQIKENLLQLLKKQIQDNCIAIKYSVEQVRTNEFQRSNQIIQTLEQILGHYSSFIDEIITYTKQQEENLSQIQEKIAQIEYGTMINTKLDQPEMKFQKLFEMTDEIKSKIETATLLQQQEDSESDDINLEEGSVKVSQKLIEKFRILDGDKCIASYACAFDNKILLQGRMYIFSSKVCFHSYFNGKTLFGTTALGIPSIDIQSIRRIKAYMVDAALEFKTLKGTLVFASLANRDQTLGSLKQVKGLESGIIEERIESDPERNIQQVYVETQQLVKPQEKQQGIRSPSPKPQNIQAETINLKNIESSNDKLQQIDQSPKILNFQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLIAEKTQQVQEVRSSPESNSKQKQNYAINQQGTTENLQQQTPKINTDCQNQSNPAIPNVSQFEVEQTTNQSNSLYNEIMVQNKSNRFMNQPNIQKFKTQLKVTVKEFIDFFLFDTPYENCLSFPHYYQVVLCQDKEISFQRYQPQPQIGQISKRPINFIHPVKEKQMFAPDVVHCFAEDVLYYFNQDEILIEKEVKFAKIPYADSFCCRVFWHIHQIEDGCQVNYGFYIHFIKSTVFKSKIESSSKKENTEVWEKCLKQVYEEGQNKILSKRVQNERQPPKAEIEVTQQIETQVEQINQIREKEETKQVILEQVQQESQILLENNENQQQKQQLLIENKVKEIFVEKQEQSSDQKDLNQKLDKLTQLAYAIIGLLIINILFTIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBE7</accession>
    <name>A0A8S1LBE7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0350127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LBE7"/>
    <dbReference type="OMA" id="QCEFMSS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="278" mass="33218" checksum="1D27E59DFC3E56EB" modified="2022-10-12" version="1">MVKFVQILSLKNILLKLNTCKETLLQQTSFLASSINKNQCLNQTHSQEMQIKNNNEILQMLEQKCQSLKPQSETEYFQIMEQTIQLVDQQLQLINKVMKKQNNRNQIKYSSLRLCLLEKAANHFYEIWQFDSENIAYLKKYLDIVKSFKGTLSDQYLEGLCEYAQHTQDYEELEQVYSQIEKNIFSEAQKRCAMTLFMNTKDEIYYEHFIINESHCLDQSLEIKFHHFATQYFQQQNCHHTTNYHRQQCEFMSSEYQRSIAQGNLELQLSFMQNKSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBY3</accession>
    <name>A0A8S1LBY3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0340079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8062963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YIHNGQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1567"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1592"/>
            <end position="1630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8062963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1631" mass="194460" checksum="0974EFC249390E56" modified="2022-10-12" version="1">MYIILILLNIVKSREIFKLEINKSYQFYGEVPNSWLTYPVHQFRLKIPQDMINKDIFVLLTQLNDTSQQIILHLSQSEQITLNQINDCRLFDLELCKCQQSQINSYCYFFVFCKMNCYYQLDVITENMNDYLSNEITLKSTNQIEGKPVMFNLSNIEYERFYIRIMQIDTFSKLSIYINMNETIPTIQEFDVQYQIDGDKYLFFQNNFTQHINTISTLIKGWGNFLLQCKTYKRIQDITLYQNIKDTVIKDGVNFYKLIIEDFKDNILIFEMIPSTKKILIYIQPCIEDRCSNNYQDFEWQFQFDQEYHQFIIPPYKMIYTKVYLIAINSKYDDSFYVFQTKINDNIQERLNTQREFQGILQKNQIALFLLQPFSNEQIDRIVDVIVNYPKGHGIVLSKLCIKDEEEIQIIDNTFEYLQQADSNNYYNCSITQEQASLISQDHLQTQNTFQFHINSSQIQINVSKLWFEYQSYNFQYVIAIQSYVESMRFSLERRLTNQQKFITLNSFTINYSLLKELNTEKYQFYTFNQPDQIFSICLATYQGEQSAKFKTFSSYYTNIIIKRVRTLDLDIMQYYAIDSFSYALQIQATKHLRYSIIPILSMKSDTLIKYIQLIQAIPFKTLDLNLYFHFEITEVAILYINLKSLQGNFICYILSSNKNFGNQFPYETNFTLTSTQQTFIIENPKQFNYLHVKSISLEEDESYQIMYYYNTSHLELFLSNTFYGFLNDNQSLYFYYQSSYQYSQLYIIKTYLSEYSDENNLQIYISLNNKCPDEFNFEYQIQPSSYYISIPNLIQDTILYIGVYSKGSNKYSILVQERNSSIQLKDNIIQISPTLEQQNYYFYYFIPKYFNQSVKIQAYTRSNLIELLCNIIEFDPEHSDKENYPTNQNYKIKELFSSNPSNKLLFINTTDLIQCKDNGCLLLINAYILGHFDHFNIMVSSKYTLIRNLEMIIGYSTQNTMSYYYFNIIEHIKDIQISVRAIQDCDYDIYLLKSQINTSILYPSVDQYTYKFDSDTLIISEDDNIGQYMIGVLAQDCIYEVLLHLGGFQLSYIHNGQIIDTFIDNTIQYFYFNNHQEPFRIMIYDIKNIKVSISIKDKSDLIELQNNDNLFGGIIQIDKDLCSICIYNITLYPIRPTEVSLILTYNTIPLTIKYGRIYYDHCLNICEFNLEPGELNLFVYSKSIKLTFHSKINQVTNMNLTYSNYIIPINQSCILRIYQDSYYSINLNNQEIPITLILGKEFNGRNMIGQNQRVFIFQIERIDLEYIIQVSSKSDIFVDVYYDNSIKKIIPKIEYRINNKQRRLIYQFQQIEKQYKIILQCIDYYHISVDIYNKNQIKYINFNNHYLEIIDETQQYNIQAVLEQDIQFEKIDCLGKTSLLKSQYVTSRDIFFKIQVTDRMKPFPFNIFSLVPHIQYSHDQWYFKNTRFEIIKQSNDSLEVKIFTMKRKKTGNLNLNKLIYKLHLSDQDYLMKALGCEIDIELLQNYTGISIFYTSIIQEVKEKEESLQFTVLINRELIYGLLVFQAYYENYDIPYTYFYNASLIYNQSETIIMKDEVGIEDSKIDYILIVFGISIILAISLICIWICMPKKKQKKREFNEQQQQQQQQQQQQQQELQSLKQNEQELVNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCC3</accession>
    <name>A0A8S1LCC3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCC3"/>
    <dbReference type="OMA" id="KWINFEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="406" mass="46294" checksum="96709FFB5C4F5A4F" modified="2022-10-12" version="1">MSNLSRSSQSSRSSSQKRNTKRPQRDKRKKNNSDEYDSQIDDEDVSQYEGMRSKNKQQPLLGPGRFNRRENKLKKPNSVYTPAATTFLYKPGAELPNLSNFINQTIEIRIACEYVNKNNQALILRQIWGSNGVYASHSDAVCIGIHAGLLALGDLKLTGTFYQGISLSCKVIKGKKTLNGQLKVPLLSRSLKTPCAHCLKPEKVNWLSQLGSPEQLISWARKMSLPPNRRLTKKTHLNVFLHEPPQSSLPENLLVNNLPPLNEGWIVWDMCNEPSQKYNLLTFLDRQTAQGIPSRTSYRLKTNCLYIETSNKKFEISMDPNKIAEEVFLDSQVFMISEIIIPQQSDIEMLQKYGVPLPKELKSLILDKLKWINFEWGNDNVIIDGQITIQGLKSFKFVPITKHQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPL7</accession>
    <name>A0A8S1LPL7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0360249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPL7"/>
    <dbReference type="OMA" id="RIMNTFN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="232"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8064684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="296" mass="34502" checksum="E5D671BE53D56503" modified="2022-10-12" version="1">MTGVNQHWTCTTCQAESLLSPCGECTNLNLRGIKENSCKCYSCKITNYYLDCKQCLTWYSVNESKDNLDGKLLSCVLSCEQVQMLKCTNCQSLKRIDNFNNEMKNYCNSCNKYYFKQICQFCREVSTFNQKESQLKQKCQNKKCSKGTYLKTGKIQNQQVIIGQFIKSNEIQQVQPQKQQSTSKYLDNLDLMNFEQDNDNTQFNQNKNNVNLLDIDQHLDQQPQNESSITKCQKCFNTTDVIITTPCGHKVTCYQCLENQLNCISCGEQISNRIMNTFNRDFYQEIKERILIYKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LT93</accession>
    <name>A0A8S1LT93_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0450158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LT93"/>
    <dbReference type="OMA" id="ECAKFIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="262" mass="30725" checksum="CA21E6642A8533F7" modified="2022-10-12" version="1">MQNSKKKQIYCNQFELFADKKFKQIPSLKLVKVEQTNHKEQIFNESSAEDIFEQMGNKESNQDQLKKVNKESKVNKNDDWKSYYNSRLKEIKLERPELNHNQLTQLISEEWKMIKKQFKKVPQPTNNVVEDVQNKKKIKQNKDNSKFCQEIIYEKQESDSELDFNPFECAKFIENLRSKKFKLVINDMTYELPGTAIIEAVDNDSVLIYLTENNNNNNTNTAYGTPNEKVNANLEDIGSDSEQSICKGNDDFILQNNIRSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUA2</accession>
    <name>A0A8S1LUA2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUA2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="64" mass="7947" checksum="8EB91C72E17A76D4" modified="2022-10-12" version="1">MLKDKVRMELQIEIFLQIIINQNNFHLRSIKIQYIEKILKKKYLDNLKKQKRKNLKYIIMKLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LY35</accession>
    <name>A0A8S1LY35_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0450244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LY35"/>
    <dbReference type="OMA" id="SIYEIRH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="44561" checksum="A98B0006457CAF97" modified="2022-10-12" version="1">MGANFLMLISGEFMDAAISVITIGIMKIILVQIASNLLLFSTIISSVLVIIYTIRYLYHFHKAMRNQFLLALNDSHWEKILNSIAFKQPYIVLSFIEDTMQFTLKSEAQCNNFFNRQFDGSNFIRDSIYKGNKLEKFLFMQIQKYRVDRASIFNKTLVVEYLRKMIRIECSIYYGNKPTILLLMRDFLKQKQPDTSIYEIRHRNFIRLLLKILSHKDRLSLLKCRLIERKLLILQLYEDLRLSKLRESEINIYNLAQIIHNLYTNLSVKAFVTGNSNINTIQNIFLLILLIIAENSQGQQLSICLTNEEKSQRWLHISGNFQIEKVKKALKSIQDYIKLISSSQIIEQFKIELNLNQYILIPFQVNQINARSLKHIDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3F4</accession>
    <name>A0A8S1M3F4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0520133</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3F4"/>
    <dbReference type="OMA" id="FLTQLYW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002483">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF139">
        <property type="entry name" value="PROTEIN SCARLET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="573"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="28"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8074209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="599" mass="68146" checksum="1966FCA41D7278C8" modified="2022-10-12" version="1">MQVYDISHAVNDTVVSAKSVYHQKAIDLSFRNINYNIQSNRGERREILKDLDGFIPGGEVTAILGSSGAGKTSLLNILACRIAQTHHVRLTGKVMANLNEYSPETFSSFAAYVMQNDVLFETLTPREAFQFVANLKFVSEDEKNHHVDEVIKTMKLERCQNTMIGGLTLKGISGGERKRTSIGFELVTNPSCILLDEPTSGLDSFTAFQIINELRNLAHRLQKTIAFTIHQPSSDIFMLFDRVMLLVQGRFIYQGSRSQLVDYFKMIGFECPQRTNPMDYMMSIMHTEKEQNIKNFEILFDSYEKFQKPEVLECIEKSSRVDLTYKKVETSTNYQIMLITKRSIKGLLREKATLRQRFGSTIFMGLLVGGAFYGLGDNNGSYSDYSGVAGCLFLLTMNLTMTFLFSVVLQFATERDVFLREENSKLYTTFSYFIGKSFVEIPFCLITPVIQELILYWMIGLNTHSVGVVFIHLLVAILTSLNGNSIGLITGCVFSDIKAATSMVSILLLPFIIFSGYLANSKYFYVWISWIQYISPMKYSFEAFATNEFEKRQYVFGDPIETLGFDIGLWESIGILIALAIFIRIMAFIFLYKLRSRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4Q0</accession>
    <name>A0A8S1M4Q0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0530086</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4Q0"/>
    <dbReference type="OMA" id="DEVELWW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="250" mass="29340" checksum="3940D179B188F362" modified="2022-10-12" version="1">MGFSCSKQKDDHIDKRIETKKQEYNLKSQSTSKEAIEFIESNSLSNIMRTKPIKSYLAEQIEKQQIQMDSCEILADRIEAKSQLIIRSFNHFTEKALKIKSIVLDYYRNEYANYINSNLDTITNKEFIQKEMIQLLVETTIILNCLMDKNFDDEVELWWGNNYFSDRIQLLVLNFNDCCLDNTDNITYPIIQYINALKNKIIHVMPVQSNQPKSISNSVAITNVHLTQFYKDLKMEFAKKVEDEDENGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4W5</accession>
    <name>A0A8S1M4W5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0500087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4W5"/>
    <dbReference type="OMA" id="VREFQCY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="147" mass="17512" checksum="CA27C4CF9A9C168A" modified="2022-10-12" version="1">MSKLQDTNRKFNYSKQRWETLTANIPNKENIQMNNNNTVKKTIKKCSTAITFQKKQPLPQRTITKYFKMTNIKKYPHLQLYDSKGYVREFQCYDDKVLNIPPAYNQTISHSMDNDCQSDNEQVESAVRQLKYFLESTLNQYKQQHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4Z3</accession>
    <name>A0A8S1M4Z3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0540047</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4Z3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8075127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="96" mass="11477" checksum="8827B5207BA58B12" modified="2022-10-12" version="1">MKTLLFFNRIIRRRRFISRIKETKYEQMAAEIMKQIFSAIIYCHENNIVHEHLATEKIIFENKKPNSNVKNIGLGFSQKLEKQSKFILNILESPCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M903</accession>
    <name>A0A8S1M903_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0520209</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M903"/>
    <dbReference type="OMA" id="YYSNGAR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000184">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="217"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8074303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="412" mass="48134" checksum="3E398CD0DDEAA722" modified="2022-10-12" version="1">MEINCKLPPRITMNPLYYSNGARPVIQYRINPNEQRRYTTDLEAMRKYSKSQNSQKQNDDNGNQQNNPQKSIGIARANTADKQQLEDSKNQYNSNRDNKKPQQPQLFKVQSQAKPIFENFSKYQSQKLKNHSFVTQHQQNEKPHSFKQIAQKGPSQISHLYYVGDLQNAILNQKSQSSALFREHIQSSFTYIPIIKNLQIDQEQIKKKLLNIPLNLNKKYNKTIIFDMDETLIHCNEDENDKCQFKIDIQFEDGEIIEAGINIRNFAKEIIQKLSDQCEVMIFTASQDVYANKVINILDPNNKLSYRIFRESCISVGDNNLIKHLGVLNRDLKNVVLIDNSSYSFAHHLENGIPILPYYHDDKDNQLIKLYRYLCQNILPVDDVRPVILSHFKLDKLNQYESVEEAVQNLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9K1</accession>
    <name>A0A8S1M9K1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0520225</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family. PFA5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9K1"/>
    <dbReference type="OMA" id="QWIINLL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF23">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="165"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8074335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="234" mass="27934" checksum="2FFD15F0C3AD8A40" modified="2022-10-12" version="1">MDFEYFDKQDDDDDMNVEFDEEDIKYNIEDSKKPIEKELDDSEISQIELKINKMKEEEQYFDQDSDEDTKKKLTQAKEWKRHFGNYIPLIFINDEPIFAISPQWIINLFVIIGLFVITYFQFTKPKQKTIQFCITIIINVLELLGYIIAALINPGINTSLQETYNKHNNCQICEIVHYRKDTYHCETCNVCIREFHHHSNFLGRCIGQGNLFYYYLSILFLPLFLINLIVLYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBT8</accession>
    <name>A0A8S1MBT8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0540277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBT8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12953" checksum="87786A62F031433E" modified="2022-10-12" version="1">MIRKIIIWFKNLRMSNQIIILNFIIILFAVFATIATAYIQQAIFFTYIQEFEQTLSLKQEKNIVNNISKKLRIYIHNFKFMAFSINGLLIYSILIRLLDQCQLILRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDV3</accession>
    <name>A0A8S1MDV3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0550031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDV3"/>
    <dbReference type="OMA" id="AFMDTID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="128"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="31567" checksum="C0E4A1F703D3B8E1" modified="2022-10-12" version="1">MDKLDKIIAQLDELEISQKHKEVQIKHNQNSLLLRLPKVILIQRICGFLDDQDLYRFTATCSTMRKIMFSPLGFKLLTLSRNAQHIVGGNKQQQQILKQEKQEIGSSGNQSENNNSVFDSEEDALAQLQALKSVKEFLTTKLQQSQAQIQNIEDLITDSSDTLKYEKSVNMKLQSKINILQNQLSISELQRQDVKENLSELNSEYTKIISQMEQDRVLLQDEKDKLLGHKKVLIQEVYRLRGMVSQMEEDQNNYQSALKQIKVFMDTIDLKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEC5</accession>
    <name>A0A8S1MEC5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEC5"/>
    <dbReference type="OMA" id="QNLMNEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="299" mass="35856" checksum="5461005D1C8018CC" modified="2022-10-12" version="1">MKSLLKYVACTTFDDQLNQKEQRPKIKKILRQNKIIQNQQKITNNLDDKIKTEQSFEENSNQENYQNNENEVESSKNQDQDGNNINSQDQESLNEQTNTQLLQQGTFTFNQQPLLHISLTYNEQNFIVPIYNYSSELTIKQSLIQLNLSQENLLKVYQICIKHLQYYLKTLETIWLNKNNEKIDNIYFLQNLMNEQSFVQTRNQNSFWVKKKELRNGQIQIDIEDKEKEYRLVFQKFKILQFIQSQKLLNIGKRFSIDLNKSDDFLKIIERFSNDNQLTQKSRDELKWLIQNELIGMFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKE0</accession>
    <name>A0A8S1MKE0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0640180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKE0"/>
    <dbReference type="OMA" id="MNKFVEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="130"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="374" mass="44873" checksum="5D239ADA3C37A9E2" modified="2022-10-12" version="1">MQKTPEKQELSQALLLSISQYSWDKQIRLTTDEDVLCSYSKFSNDQGSYCETKKDQGNVFIKKCNQVQIDDVQSQENKQLLLILREKDKRIEELEHYQVWLQEQNEKLKLNQQMMIEDQSMNQLQRDSSSKRHLQKIEDLELQISQSEQQYHKSLKSLGQLEKENSTLKTKIFEYEQKLFQQQHQQKVTEQINEQQKLKYEEQEQINQSKIYELQQYIQSQESYINKLQHDVQLSRKNSKENISKQYKSVVLREKSPQLNQYDQHQMNKFVECIVDMVMNCTLSQAQTKPSLKECWKWLKNILEEYMILKKQILQDTNKTHSTKSSQFVGKEINGERKRDHSFFNDLNRRFQTSVEDSENKYLRSNTLLSRVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKS0</accession>
    <name>A0A8S1MKS0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0620162</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKS0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF9">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8079712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="27540" checksum="9CB3CC80CA95C3AA" modified="2022-10-12" version="1">MENQDYEHDSEDEGIEDNLIGGYHPVHFKEALLNRYVVMQKGRFSTVWLAQDFQHDTLVTLKFKRVHLNIWKLYKIKQQKPNLLEILQKIAQNVYKSIWIQSLKEYYANVQIICSNNVDDSHRLALLKKYINQRFAFQSFRISEISFQFLDCQRPTLINELGIKCIMRNLRCILNQREKMKKRSNLIRILEDLITFILIKQKSMKNHLIIWHQLDLMKDLIHKVLNKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MMZ5</accession>
    <name>A0A8S1MMZ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0600138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MMZ5"/>
    <dbReference type="OMA" id="YWINQRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8078573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="104" mass="12909" checksum="44352700E34F0DE9" modified="2022-10-12" version="1">MVNFTISLLNVYKWSILMPRLQVNRLQMFLGILKQKYPKMEKNKQFIQKRMEMASLMKNHVNQYWINQRIMLRLESLLQKQIIYWISTLNINEETKFKYKKFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MMZ7</accession>
    <name>A0A8S1MMZ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0660205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MMZ7"/>
    <dbReference type="OMA" id="WESKMLE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="453"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="831" mass="98823" checksum="5F3BB3722CE58AB8" modified="2022-10-12" version="1">MRPQSPKILQTQTIATTTRLISSPPRDTRQDSCSRLAKITTCQTRPKATITTTTYRPAIETRTVVLCTDKKCQGHENLIEQLTQDNNKLNQRIYELENQIDKYDGEQQTWMSTAIEIESMRKLLEETQKNHQQEVQHLNTEIYNLKSKCQNQEDKIKIITQQHQNLDANYLTLQQDMERQQFIIQDKDEQLSTQEYRIQDYEKQLQVGENVIEERNSQISILLDKIKQYEQEYITSNNVIHQLKQEISQKDIQNQQLLQQKDSLISNYKENLDLLEQKVQQDQHLDAQNIQKLAEFENQNQDLNNKVLDLQQQLQDILEQNNHLNEQVMLNDQKNANLLNSIENQTKQLEFQLQSQADEHKQQNNEQLHHLQQELYNTIQKVEDKDNLINSLNDQVVNLTTKINDKENKIELLQYEITQHFAQIAEYNNKELYNYLIINEELKKEIQLRFVKEKQIQNDLNKEIENNNQLLETIKQLNDKIQELDKSKQNLANDLQGQKQQLDDQNLETQKLSEQVQIQQKQISDSLQEKESVEDSVIQLKEEINQLQECLLQSKRTEEQIVNGNQELQEQLLLQSLEVQRLQGENKNLKDKLDQQFEEVKKLNEQSTQVIENKQLLQEVQDKQIEQFKVIIQDLNIQCQKLESDLKQQVELNEELNKKNSFCQVQISHLQEVESQLRYLNEQTNQQQKNLQQELDNKNEIENTLKFKLETLLEDIKVKQMQLVELDQQLQMVEQTNQKQQLNLEEKINYLQNEVEMWKEKFVILNRDYHRVQEDLMMVQAEFEAFNKRGLEIKNIKESTYFEVRKSSLYKENIDIKASQTSIGRLFKENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPE7</accession>
    <name>A0A8S1MPE7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0690023</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8082927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPE7"/>
    <dbReference type="OMA" id="YNGREFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001133">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="64"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8082927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="443" mass="52162" checksum="9662D7711C0AFEC6" modified="2022-10-12" version="1">MQQKRNQEQLRNALQQELFPKMQKKIEQSKVKPKRAIRIEDSNNRLPQVFPQIESMEIDFQAKYRLGERIGQGAHGLVKQAIKEDTGEIVAVKISSSGDPELVKTFTEAYKNARILNHQYIIKVYECYIDEQSETLFLVMEYSNLRSLEEVMRHTKLTEEQIKILIRNILLALQHIHERGVAHRDLKPDNILIDQNSLDIKIIDFGVSRRFKKYNGREFIDVDMWTRTGNIYYTAPEILIGGGYNEKVDLWSLGVCVYRILSGNFPFFKDSVLGTTKMIIKGKFQLDNQISHLAQDFIKRLLNPNPIQRLSAQLALQHPWLCCQKFDRFSLLSSQYPKSIYRTNDDIKDFNQYEKKNSYYSRNLHMRSNTMSKSPIVQHDRQVSPKSPLEILQYDIDGEIQQSPLIKLKKIQHQMNKEINIIKRNKQDDYRFFRFRMINKNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQ21</accession>
    <name>A0A8S1MQ21_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0610100</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="INEGHYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000015">
        <property type="entry name" value="Oxysterol binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000100">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF148">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="START" evidence="4">
        <location>
            <begin position="568"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="254"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="317"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8079056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1296" mass="153187" checksum="E690AA010D924772" modified="2022-10-12" version="1">MDFDFIEAQNSFQFLQEQKKLPIKMSHKLELLLECINGQDIDTSIFHSLDLEECHALMKLNKQAQKEMFMQIIKGYAYANMIPSAIGEIKGIIDVRTKVLKSWKQKQFKIDSKQRTFEILRKKSSKKKPRIIYLQYYTVQPQEFKDKRYRFVIIANSDGHGYYKTLVCGSDDKALYDKVIQALKTISQFTDHVMRGSILLESNDFNQPRSNTNQQSFVTTRQDMRLVTQSEIIPQKQNTNIKITKQTFDLDIKPNQQQQQKQQQQNLPQNIQQQSNISQQIVQNDSPAKLQQQNQYIQQQQSQPAFNNQQQYPQQQQSQQQQQQQQYQQQQQQQQQQLQYQQQYQQQQQQQQQQQQQQQSQQYQQSQQQQLLQQQQQQQQQLQQPQQQQQQQLQQQQQQYQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLQYQQQQLQLQQQQQYQQQQLLLQQQQQQKFQEEQYKLQQEQLLQQQKIDQQRQQQELIKQQLLQQEQQQKIQSQVDMQQKNEANLVKNELKYPQVFQVLFEEGKFNPDYFVQSTKQFTQVYSKDGVQILKDQNNSLCFRSSLTIYNGDMEKVFQCLAEIDFVKKWNAVINPNETKLLNMMQSENCGIMYERHKPYGLLYLPRDFVYLRYVTYRGDDILIVDKSIENEDAPPYMKVIRGEINYQLTEIVKSENNIIVRIESEITNGGLSSVNQDCAITQWYLSELSHFQQFLKTYSAVQEIIQSPLLQVLNRTKVNKQKENIIIDIGVTNIHQQQQVQPPQQLQQPTNFQQQQQQQQEYLSIQQQQQVLKQEAYQPSPITVAYEEQFKFDKINETQTITLDQLPIIATVPETDPLIIENHKEINEIQQYNKDIEDADLSNYTPSDFIQQTLPELSDKEIGQVKQMIKNIEQGLYDYTANCINHKYVTEPQKVKDEYITQINEGHYFLRQDWKRDMKHGGMIFYNQKKIEAQKRVIKFMLKSIGSNLLSGKSILNISLPVEVFESRSNLERFCYSFTFAPQILEKAAKIDNIIEQMKYTIAFGCSSIIMYMSLEKPFNPILGETYQGFINGCPVYAEQVSHHPPISAFQLKGRGYDIDGHIESAASMHANSVTGKNIGYIRVTYHNTRSKQIFIQCPGVLTGTAFGTRVFNINGRSYTIDLENNLIADMAFNPGSKNVFNKKDLLQDQFIGYLYKVKPGVLNKFKKEGYAKYSGIDFDKDVEQRYFSINGIWNQMVEFDGVKLFDTLEMNPHVMVNHPCPLPSDSNFRLDILYWKLRDFNNSQRTKEMYEVKQRNDRKCREKLTGKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MX27</accession>
    <name>A0A8S1MX27_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0710008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MX27"/>
    <dbReference type="OMA" id="HDIDNQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="106"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="192" mass="23091" checksum="04D185C680280C71" modified="2022-10-12" version="1">MSINQQKFDTNFYTKLNNLEQRFYQEEADKQDLEQLILSIGELVMFYDLKKDPIKQYFLEKMQFILSRPFTLLNLKEQYLITENKTPIGHRHQHNFSLPVLKLEKQAQVQSDLNNSFEKIKTLQEEYEMQQRDQAELIQHDIDNQMSVLKKKIIVRKQNRNNHNHVYHHSNFISNSKLSDTKTSDEEQTKMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZJ5</accession>
    <name>A0A8S1MZJ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0750090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZJ5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="123" mass="14839" checksum="4B76FD21148DAAEA" modified="2022-10-12" version="1">MGCVAQKKKVEKQIYRMETQIKKLVQQNEEMTNQINTMREQEQLLSQLCEQRSERQRIDLTALTDELNNRFEKLRNDLKQIDIICEEQKQAKLKEMLEWKNLFDKLDNVSKNNLSQISEEDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2M4</accession>
    <name>A0A8S1N2M4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0650113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2M4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8081224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="153" mass="18319" checksum="5F5B03D2E0637399" modified="2022-10-12" version="1">MLCKFHFLLGNYHRMKCIQCQIYNHYNELNKLHRSYYLNNGLFCIMNNYWRKFHITSKMDRSFHILNHQDNIHFCKINNLCFLHLNNLHMDVHTAGNQMHDEQLQVQSQSQHISSTNLFQYLVITTTIIVNQLITKRAELAFINSIFTINTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6C2</accession>
    <name>A0A8S1N6C2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6C2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="128"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="231" mass="28018" checksum="B50CC6C4D2338A65" modified="2022-10-12" version="1">MLNTIFSLQVFRNLDIKAMIQAYDYALQNINELSNQIEQLEKSAIDIIVEKDQIIEQLELQLLHSSKRTSFNNQEEVVQYKNHIQELQIRLEHLQNKHFEQLQEQEKMWNQYLLDQIAQNEYTIDQKLKSHLEQIFILEDKINKLENELEIKENMQISLINQNKIFKENNTKLEEVQMTLNKQLLKYQIDIQELQQNYFKEKNQYLEQINVNLTKFILFSKYSYLRLNGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7V3</accession>
    <name>A0A8S1N7V3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0820111</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7V3"/>
    <dbReference type="OMA" id="QLVLHEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="235"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="472" mass="54900" checksum="47EFE9925D609C22" modified="2022-10-12" version="1">MNNTIQNIRRPVTSQNCRSIEKLDKKNSDKLPNLSEVLNLKLKQKSEKDIQELITNLVKKVQQQNSELALKEKQIQQYEFMIKSLQIAIEKRDCIISALRVEKQQIVEVVNPLKTIVKSDDSSVQQSFRGKKSLRTRTKDTNFDENLSAILDADYALLSQYVKCYEELSHLTQIKTLVSNEEQFLVNIQKMELSNIVNIFDAIQLVLHEHKLLFKNVIKQNKLFESCLEMHDQIPLNDQLENLQSLLKDCLNCDKVQIYVLDNEQQELWTKMKDRVQRIQLSEDIIGACFQEKGIINVHNAYNDPRFKKDNEKGYKTNTILLCPILDKQQNSIGVIMAVNKMNGHFNNDDQLYITKTSELTSILLRNHQQSNESISIQNALRNVIYAQLQLIYMNDFETILFESENLLKNLFHTQKGLIYVVNNNRLLRVNEKKLLEISQLGVGIIGEAHKNKEFLCIQNAYNHKQFNSLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N8F3</accession>
    <name>A0A8S1N8F3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0760135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N8F3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="27535" checksum="66A5FD2D2CB208C0" modified="2022-10-12" version="1">MSIIGCFKYIRNLAKVYIKLAELDKQTQKIQKNHYNCVCNLKIQKYLEMLLKLTFYQEISHYKMIQKEKKKKHQVFFESSSDIRNSFMQNNQQRIKINTYDRQHFKLSFLDNDDIKELKQVLSMLYDKIEDTNVAQKEIQSDEWMKTPYEMLNKAQLLQQQQIQQQQFTQQPIQEERQIISLGNFRNARRKLDIVKQQSQNIVVQGNNQEKQEINQSDEQCASKKVKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAM0</accession>
    <name>A0A8S1NAM0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0840168</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CELAENK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="334"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="383"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="425"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="467"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1072"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1114"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1156"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="1176"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1198"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1240"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1282"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1324"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1366"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1408"/>
            <end position="1449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8089850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1531" mass="170712" checksum="D17DE33EFB694FB4" modified="2022-10-12" version="1">MKQENYKNGTQFYLQHVQVNFSLKEVIVQMIQLILLELKFSLKIFIQKQRYSILLKKSKNANVEMSSQSPILHQSKPYTVSEPLFKKMHKILLSILKYITQNSNNRNSMLKITKKIQCLFQDKQALAYIFTQVKDVEEQIYGVMLEIFRKEKVKDCIEYLLENENQKRIEQTILQQGGNCELAENKQKLQVARNEMRIISDALKKIKDHEFNKKDFSCVEKQESILNLIKSIKDNIQIIEYLKFLVRLTSIDKTMIQCGSNSLYVLVQMKADLRNQNFENIKIQNTSLVGANLIRCNLSGSEFNNVSISGINLNEALLWYCKWKNIKLHELIKLDGHHDNVNSVCFTPDGTTVATCSGNPVYYSIDNSIRLWDVKTGKQKAKIDGHTSTVNSVSFSPDGNTLASGSGDKSIRLWDVKTGQQKAKLDGHTKGINSVCFSLDGNILASCSYDNSIRLWDMKTKKQRAKLDGHTSTVNSVCFSPDGNTLASGSGDISIRLWDVKTGQQKAKLDGHSGQVFSVCFSPDVVISLSVYGMSKQDNKKLNWMVILKESTQYVSLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDKSIRLWDVKTGQQKAKLDGHTSNVCSVCFSTDRNTFASGSGDMSIRLWDVKAGKQKAKLDGHSGYVMSVCFSPDGNTLTSGSQDNIIRLWDVKNEQQKVKLDGHSGYVLSVCFSPDGITLASGSDDKSIRLWDAKTGQQKAKLDGHTSTVCSLCFSPDGNTLASCSGDNSIRLWDVKKAQQKAKLNGHRDYVRSVCFSPDGNILASGIYDNSIRLWEVKKRQQQAKLDGHSGYVMSVCFSPDGNTLASGSGDKSILLWDVKTGQQKAKLEGHNNIVMSVCYSPDGNTLASGSQDQSICLWDVNTGQQKAKIDGHSGYVMSVCFSPDGNTLASGSGDNSIRLWDVKTGQQKAKLDGHTKGINSVCFSPDGNILASGSYDNSIRIWDVKTGKEIKHQNNNTIEILQKYTTPLLQNNPFTNFVNPNIPILVISENPYFLSNGALILQGEFQTTWGEDLRAFFESRGGCILENLNQNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDH5</accession>
    <name>A0A8S1NDH5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0860155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDH5"/>
    <dbReference type="OMA" id="YMIALTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000159">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000108">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="360" mass="42588" checksum="C95D32CBCB806502" modified="2022-10-12" version="1">MKSILNQSKQNLMLHVANSIIKNAKNDFTVKDCDVPTINKIPASKRIQTKPKENALSEYNVITRCVTEQRKSSVTETSINEQKEQKRIPSYCMKWLNYKYQKKEFVQLEQQFLKILQIQPEKDVVHQIERDLSRTIKAENHFKEIRNILTAFSVYDPNIGYVQGMNYIASILFNHAKQEWIAFWLFVSLIEQMEIRDIFQLSLNAINKYSKILDFLISRFLPKAYENLCLKQVNTELYIQQWILSLLLQFIPFEYTQMYLDGLFKQGISYFYMIALTIIKVFDKAIQEEDQIEILILLTSKQYSQLKWPSILALQWDINPGELRVLLDCFDSDTNTFNKPKFQTHFSQLTTNFFQFIKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFE8</accession>
    <name>A0A8S1NFE8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0850052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFE8"/>
    <dbReference type="OMA" id="ISDMQQC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="393" mass="46496" checksum="31CEBCE2529CE6DE" modified="2022-10-12" version="1">MRKSPLMKENMKAIHNQKFSYLNKSKQKTSSLEKNSYSNRSVLNEMQHQTPNQPKKNISTQASDQKLHNQSQILKSLMKIQNRTNPILAQQNSYAGVKLNFNNCISNEEKRMNTNQYNSLNQTIHPSPKVKTISLSTYTSTQSPEIREKEIRHEQISQFKQLIIQTKQSLENVRISDMQQCIERIFDQTIQPLLQLKSEVLKQIEKCINAEQCQIRSTIESLKKMEEDILSNEFNIIEYMAMGPFNEIMLIYQKRFKEHSIFVQQLNQQDIVMHQFKQLHDNFTQQNHLLCKQLQQSLQNYLTLYISFQNKEDFYEIKEFNKIQQQNQFINNFQENLFQTCETNNSIDEDQVIMKDQQVVRIPDKTSQFTQLLMKGLNCQKSLFPSESPQFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFX1</accession>
    <name>A0A8S1NFX1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Aminomethyltransferase</fullName>
            <ecNumber evidence="2 7">2.1.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 7">Glycine cleavage system T protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0850142</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GcvT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="2 7"/>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFX1"/>
    <dbReference type="OMA" id="MPVQYPA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006546">
        <property type="term" value="P:glycine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006223">
        <property type="entry name" value="GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GcvT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001567">
        <property type="entry name" value="PRK00389.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF2">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006487">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="GCVT N-terminal" evidence="8">
        <location>
            <begin position="16"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Aminomethyltransferase C-terminal" evidence="9">
        <location>
            <begin position="301"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8090189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="385" mass="43742" checksum="1221E1C43B389A13" modified="2022-10-12" version="1">MKKISYSFTQLMKLHLHDYHVNLKAKMVPFAGYEMPVQYPQGVLKEHLYCRESCGLFDVSHMGQVKVFGEDRMKFVETLTTGEFQTKKSGQSVLCLILNEKAGIIDDTIVAKRDDHIHIVVNAGNKFIDMKQMDKIIKDYNYKVQYEYLKDKPLIAVQGPNAHKVLNEVFGTEYNLDKIPFMFMVNIQKNGIDYQINRCGYTGEDGYEISVENSKAQELCDKLLATKMAQFCGLGARDSLRLEAGLCLHGHEMDDTISPYEAKLMWTVRKPNKETGKYNESASFIGRDALPQRQKDAKFKRMGFITQSGIARPPCDIEFQGQKIGSVTSGTYSPNLKKGLGFAFINNEYAKDGTQLQADIRGSKVNITLSPTPFVPQRYYKPEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NLL2</accession>
    <name>A0A8S1NLL2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0850236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="RIWGTSS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="188"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="230"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="276" mass="31255" checksum="36D1F67430DF2D3E" modified="2022-10-12" version="1" fragment="single">MRTFKNQLNFQIKAHDFNNKSYSNEKQEKTKLVLLLKKSHNVIFIQLLQLLIQLTDFYKLFIQSGSKSLHLLVDMKVDIRNQSFENINIKNTSLLGANLVQCNLSRSEFNNVTISSMNLNGTFLLNCKWKHIKIHELNQLDGHNDNMNSVCFTPDSTVATCSRNPRYNTIDNSILLWDIKTRQQKAKLDGHSDEVRSVCFSPDGNTLASGSYDNSIRLWDAKTGQQKAKLDGHSNCINSVCFSSDGNTLASGSDDNSIRLWDVKTGQQKAKLDGHX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTU4</accession>
    <name>A0A8S1NTU4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0940007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTU4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="2">
        <location>
            <begin position="35"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8093691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="160" mass="18271" checksum="49948E387E00F100" modified="2022-10-12" version="1">MVLLKSYLELRSTRRELKQIYLLQIGKVQLLLKIKVIVELLGLLQQQEHITNQDLLEQQLIDCDLESQGCEDVNLDNSLNRVFKNGVTTATNYPYTSKTEACKRQTGQFKIKGYQKVDPNQIQSAIIKSPIAASVDGSSWTFYNSGVFNRCTFEELNHDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NW51</accession>
    <name>A0A8S1NW51_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0990183</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NW51"/>
    <dbReference type="OMA" id="MANTQQS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="152"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="245"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="486"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="97"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="491"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="503"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8095850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="541" mass="63907" checksum="1AB5D3F39A5EE435" modified="2022-10-12" version="1">MQQNNSAIKHLIKPFSFELPQSEQFNPLLLDNLNWDYVQANLDIAQCGPFLKNFVQSNFTSDTIRNMEFRQFKKMLEVCQFAMDFYTQNQEQKEIEIDEKVQILRNKQEQLEKLKEIEKQHMDKQNQINKELELLKQEAKKVKEQAVKNIIFKCHHCDKCYSSKQELLKHIDNKHVEKQKDDITNPEYLGSMLQQLQQQQTQQQQSMANTQQSFMKQQQFGNTMQSMPDPLMIGSAGFQQLKQQQIEKMEQKQKRKEERKIKRENDREERQKRILQQEEQMKQFQNQMKILIEQKVQEMVKLNNMVGSQQQAQIIQQKKLIAELEELKQMRFVQKDHSIIQQEQQQVNQSKSNEDEKILNIIDKEVNDLDISQQSLHSQQLSRDQKLQINKNQKAGDQSGMMSFHNYSYSQNSQNMINVKTEMGKANQIKNNQLNVAEMAHVGEIESDNEVLDQDGDASYLRPVNKKSEVQQAEIEFIEAIRKKKILKEDPQDDDDEDDHDFDDLLNKEKQKLLGPNSESQTNKELLESLNKIQQLSKDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYB7</accession>
    <name>A0A8S1NYB7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0970010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYB7"/>
    <dbReference type="OMA" id="EDFIEQC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="180" mass="20883" checksum="D992B45175E9ACF8" modified="2022-10-12" version="1">MQILSDLQYLQDKFSQQSLQIRQLFTEIQQAQQTLSSLRKPVGQLRQSKSTIILHSTYQDSNQSPNKIVRNASLKQEDFIEQCLKKIEQANKTSNTNTPSPQQPKQANRIKGRLEKHIPEMKTQDEQIPLPTTSLQSIFQASNSPQQKLSKLMQLDDFKITTKQQKQEYFDIHEEGQLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZU0</accession>
    <name>A0A8S1NZU0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1010068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZU0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="20707" checksum="69D7DE52DA07EB5E" modified="2022-10-12" version="1">MTLQDGKYYKGEWDNDERYGQGEFHWGPDQYYIGQWVENVREGTGEYVEEGTKYIGNWVSDKKHGQGKQIYPLYTYEGSWIANSREGQGTAVFNNGLKYTGQWMNDCIHGQGIVEYKDYKYSGDWSRNRMDGNGVFTHDFCTYTGQYRSGVRVGVGTFKDGAGNLIKQEFRNGDLVDNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P190</accession>
    <name>A0A8S1P190_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1010045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P190"/>
    <dbReference type="OMA" id="EARMINN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="130" mass="15393" checksum="8B3EFEF362796C2A" modified="2022-10-12" version="1">MKSKILKTDQIFDILQLSNPNYDSPMNFRELNDSIEIYFNTEEKKTEIEIIKYLNNMGIQTKTDPLQKEQEYWKRKKLETQQKMLSILCLTESTQSLTMNGYASKLLHHRMSQSSFEARMINNTSTMIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1J1</accession>
    <name>A0A8S1P1J1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1020003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="339" mass="40501" checksum="1C2F5A685B527259" modified="2022-10-12" version="1">MNCNHHIKNPISFICVAPHICSCYRKLCAECQYDHGVNVKQTIPINKFQDMAIKKLKDSKLDESSELTKQRIAFKSLISKTELMLKKIWEELSQSIKQGYDWIEKENQSFLNLINENTNLAESSYNDLEKLVQIFERTTLNDWNDQKKYYLTELDKIKIWWDYHIKAFIEKSKEGIQLFQSIIKIQTYEWKEDLYDIISQSKYIDTLIFSLIIKILNKEKISDSIGFFDQNDNQKLYQQYENAEVNLRDMLWSIKQLKKLMNNLNQHEFSQKEYSIYGYQKIRQNLIQKIDQDKKIIEFLKFLVHLTAFDSKFIQCGSNALNLLVEXYILKIQLILKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5W8</accession>
    <name>A0A8S1P5W8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1070015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5W8"/>
    <dbReference type="OMA" id="YKRQYLQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="261" mass="30310" checksum="449E30B8F3AB7EEB" modified="2022-10-12" version="1">MFSSISVSLQDLRGQRIISTNGISNYRNFQRMPITKTNYKRQYLQGASNRVKSLKEESNFIQLCSDRKDNEKQFKFNKQSQLLNYRNFHLHKQYPLTQREQQNEIDSSNKINSGKQMFKNSSLKLITDSNNQLIELVCSSEPVIQKNKKTNSISNVQKVEERINNNNNNNNNNNNNNNNNNNNNNNNNNNNNTKRISSANNHLQLIISSTNNKEDLLIKSTQSTQNYISKVLIKENKLDRSLKTKIPTSFVGTKKFLKQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PB61</accession>
    <name>A0A8S1PB61_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1120081</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PB61"/>
    <dbReference type="OMA" id="HAWQKEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="356"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="4">
        <location>
            <begin position="374"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="789"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="173"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="796"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8100376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1047" mass="124149" checksum="9DFA324F4E09FB0F" modified="2022-10-12" version="1">MQKIDQLHKYNIDLDLELRAVQNYDFEQEYLDHCLRDTHRQLSINNNHHIHSQTKSEKSNDGYDFRTPKVTSQTNLNNIVTSEVQQNHTITLKYVNQEKPISTGSSIMQFFFIRRFLEKLTFQRKRLQNLNITHLNLIDDKASDKASLLAYKQTKIQKGLTINQLNRIVQTKIEIKERKWENVKRNLQESKQKIIRQITNIANKIPLVQPENDFKLYWDIFASLFRVMLVILVPLEISFHTQILFENYVGLTLTILFILQLDLFIRINTLCYENGKAITDRWEIIIRQVSKSWFTDFSLIIIIIVFIFNSNAQDGYDLFLLILLTQYKHIFEALSKTDQISYFTRPQRGIIGLIKFITSLFYIMHIFSCIWFWFSSIDKSNSWIVTKELDGQTWQLQYLESIYFAIVTMLTIGYGDNVPKNSIEKIVTIIFILGACLWFSYSVNFIGTIMNDITQNQVERSQKMRVINKYMTQRQIPFSLQHQVKEYLQYRWKEDDEVDLQMEQILLEQLSDELKEELEKQAYKVFIQKSELLQKQFSLEFRNALFKSIKRKIIQPQHTFQSEINGQQHLCFVEQGVLLYQHKDRKQRSKMNATVHQGQFFCVKEFLMQTPEYEIFKAQSYVSLLVLSKQDFLETLKDFPDDLQKYCQLKDSYSQENEGCQISFSSFCPACNNFDHSLRNCCQIQLILNKEVIIKKHQMNQESQREVYSRKTSDKPIQTRAELTYVQECALYFQTENQGLINEQLKLQIVQEQEIESYQGNSETHNIYDPIPSVNIFQINEMLQSTHKPIQNKTFPKVEQEEEKQKEKNENQKTNVLLTQSQRMNRKGSLFQKQLQRKNTKSPSILFSMNEIAEEVNETTFIVHESDNIKNTNNFNFYMKENIRSLYTKLQRLSDDKSTTINEALNSLQYIYWKYNSITLEGFESLFNYDYYFPESNSINTIEMANKNQHAWQKEILQKFTKYLFYPYLFINKFLKLKRIQYQSAAINAATKFKNAQKKIWLIQLRNNLSSKKLISSSKNIKLTKSKMNSIVPYPGDSMQSIQISQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PC31</accession>
    <name>A0A8S1PC31_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1120024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PC31"/>
    <dbReference type="OMA" id="IIILWLH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF40">
        <property type="entry name" value="POLY(A) RNA POLYMERASE GLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="1">
        <location>
            <begin position="15"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="333" mass="39488" checksum="235B06FC2AB4DE58" modified="2022-10-12" version="1">MFNIQQPIEIESCLEQIFQKSLSDQQLEELINDSIQAFKIFLNNYELAQRNIKIIVFGSILNGFETKQSDIDFTITTNSYIPEVKALQYWTSQLEKQTRFKVEKSILNSRVPVIKLLDQQNLIYIDLCYNNLLGAINTRLLKAYSQLDEKVKKGWWVKIISNFLFSSYSIIILWLHFLQTNYGLPNLQDQEYNYDNIDSDLIIKRTLQENENIIKIKTFFVYEGQTYEKLQLQFQQRISLISLQSLLQEFFYYYSQNGEGFNQDYKISINLKEKKDPGVQYSMSDPFDPLHDPLKKINKLFKNNKAFDNALLFLKTKQDTEYLFKNENQLSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PE34</accession>
    <name>A0A8S1PE34_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine carboxypeptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1150010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PE34"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF201">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="21488" checksum="7CB16546E2B39115" modified="2022-10-12" version="1">MGCKNKKINFIRVFHKKKFSTSYHSKQNQKICSVKPESDQICLGSNINVYNIYGYCKEAPEFLKPKTKTIKQIRYSYVSWYEGNNFYDDDKRKAPCSDFGPINEYYNNAKVQEALHIQERPYFWSACSQEVNKAFNISNNGSYQILPLLSQSGIKILIYSGDQDAVINVVETEQSINMIPDSIGQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PEL5</accession>
    <name>A0A8S1PEL5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carboxypeptidase</fullName>
            <ecNumber evidence="1">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1150151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PEL5"/>
    <dbReference type="OMA" id="PEMAHID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000253">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF201">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036516809" description="Carboxypeptidase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="460" mass="52360" checksum="163D2BADBEB91677" modified="2022-10-12" version="1">MMSLLITGFVIGIVYAVNPDTDRVIFPGWGEYNFNSYSGYLPIGAGLRQLSYVFLESQRNPLNDPLVVWLGGGPGCSSLLGLNEEIGPFVMVDDDREFKKNPYSWNIRANILFLESPAGVGFSINIDTKYEYNDENSGQDNYQALLVWFQAFKQFQKHKFFIAGESYAGMYIPYTAKAIVEGNKQAVFKIPLEGILIGNGLLIWDAKIRRSALQEFFIRRNFLPPTTANTIRKICGVKPDSINCVLAETQFDQICLGSNINVYNIYGYCKEDTTPEFLKPKTKTTKQIRYPYVSWYEGNNFYDDDKRKAPCSDFGPINEYYNNATVQEALHILERPYFWSACSQEVNKAYNISNNGSYQILPLLSQSGIKILIYSGDQDAVISVVETEQSINMIPGIQELDSWTPWGNTDKDLAGWITQYNYLKFIVVKGAGHMVPEDQRQNAFEMFNSFIYGNELPKYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIP1</accession>
    <name>A0A8S1PIP1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1200055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIP1"/>
    <dbReference type="OMA" id="CNSNEQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11681" checksum="CD7A179230434E5A" modified="2022-10-12" version="1">MNSSPYIVKRKQRVGDWICGVCKNLNFSFRNQCKSNEQYPGNRCNKFPHRKSSKQQFRGFRTLVLIHYEEPIESVDELLENQIDPIVKSSLLVLDMDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PK25</accession>
    <name>A0A8S1PK25_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1210075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PK25"/>
    <dbReference type="OMA" id="DEECKIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17890" checksum="D652871566A7C41C" modified="2022-10-12" version="1">MNTEYQQQEIELQRQSHQNSEDTNNQLFSIIFAIIYNFIWGILFYIFRHLYYEEECKGMNFWSFIAQIFLFSVAIYKLWKQNLFEITEKVEFVLSIIVLIGLSYAYFQFEDCYGLRNFVLFYLIVTYVVLGIYLISLLLLILNKSNNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLF8</accession>
    <name>A0A8S1PLF8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1220159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8104037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLF8"/>
    <dbReference type="OMA" id="FEEFSFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="201" mass="24034" checksum="D7C61DCE93EAFB8E" modified="2022-10-12" version="1">MSKEILPIIHFDKQTLTTKSKTQQSSQNRNLIYKTLYVPKDDKSSHINSSIKTENESYDYVINQLHQHRQLRQPPSRLINQLRRQKMNVDKMPRPKAKKLSKPSYSINLNTQDKNQLQKQICNYVQQANKLQQYKLSAIHSRRQDFKPYFNFEEFSFMDEIFPHSNLVKKKFGLNEQIMFRNSMGLEKVILQRIIQTQEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLH6</accession>
    <name>A0A8S1PLH6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1210197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLH6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="231"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="362" mass="42167" checksum="AFE062714A58F4FD" modified="2022-10-12" version="1">MIINITPDETKSFRCFQQQKVQVVVQEEQEVFERQEDLLQILSNVNQINIIDDDDSSSIFKKLLDMLKKEQIKDCVAYLQSFKYQDQKEKEQIKKIIHILKNIKDREFNQKDYSSEPLEEIKKDLIKKLQGNKSMIELLKFLIMLTSIDDQFISCGSNSLHMLVMMKVDVRNQSFENIRMKNTSLISGNFVRCNLNGSEFENVDISGVNFNGAQMFNCQWKNIKVHELNRLDGHSNCVRSVCFSPDRNTLASGSYDYSIRLWDVKTGQQKAKLDGHTSTVYSVCSLLMEIHQLLVVVISLSVFGMSKQDKKLNPLTKTTKIFLPNLRFHSNNIDIFSKEPPIISLKTLYPNRQYFKHKEHQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PNE2</accession>
    <name>A0A8S1PNE2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1250115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PNE2"/>
    <dbReference type="OMA" id="IPGMFQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="150" mass="17834" checksum="BAEF6774B2221D0B" modified="2022-10-12" version="1">MNKSESFHQEVLSLKADSEILQSPEKKDLRTRNNIIPGMFQEYKRTYIKVPQESKEQLHQLVFKEGFKIKEAAQKLFIKYATAKTIVFHLRKKFEKERKQGSKFCRYIQLKGKISIKLRIISIIQKNLISSVEYTVQHQSNDQPYEQQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PNP9</accession>
    <name>A0A8S1PNP9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1250063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PNP9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035829328" evidence="1">
        <location>
            <begin position="23"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="363" mass="40759" checksum="B807CEB488011DD2" modified="2022-10-12" version="1">MARIIPILTLILAVVTARVAKSESPKEVLAQINKDSFGNSVLSVLQLQLATGGPVGEIQILLNNIASQLNGDQKKADKVHESDTVAFEKIIADLEQEIAYHQTQIVALSNLRDSTTEALGEAEAEVRVVTSDIANNEKSFADESATRQSQHETWVRKDAEHVDQMDAIDEASKIVQHLQAGVAFAQLKSRFEKVQAKLMESKHALFKPLINALTQLASKVDNKSIIKILELLAQIRQQLVASRASLLATEERQAANWEVQSGHLQEEHKRLVERKAFLENSIVQFKVTIQEAVEDLEDQTLFLEDAEDSLAIQERWAAEQESQYEAQTFEREQQLEVVERLQEVLTQKMSAASEFLQIREEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PNZ5</accession>
    <name>A0A8S1PNZ5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1260099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PNZ5"/>
    <dbReference type="OMA" id="PGENKYT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="895"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="936" mass="112001" checksum="C0B1620374D3047A" modified="2022-10-12" version="1">MSISEIIPIVDSQYFFGLYKSELKLFQVQPPKYYHSFNTSQRIIFLIFQRWTYLTKFILMQSNIEILNESVPRIIIDKKDMIIESHNNDEICILQSKITQSLPINLQTIQDNDRYYQINNILNIETHNLYFGEYSKVLNFWKFNDQTNVILVLIQSKDLIILIFCKQNKILKKQNIKIKQNYIDIGVLQNSNILVFYEDQIQHYKIVDSQQIQKTQFVQDQKIIKLNKLYNQIVISYDNCKKSVVKSIDNNFIFMQQPQNYPFDCTQMQQISITNLYIFQTYIVYNLNHFKQYIQVGKIIDILIDVDQRLYVLILDEEDEIVLKLFRFLRTPDFIFIYKIPTYNFKFQLSVNQKIENSYLMITAKYQNKTYLLVYNLRNQAINSLIYITEIDQSNQFYSYYDFTSNSIVFIKDGKFYFHKLNQICFKYTFFEENFMIQKKQLKLNFNSFINNFNFTTKLLVSKLNSDYTLSLLKQGQKRNIYYQGNLDLNQIRGNIIKFEIQPTQDFIIKLPINITQEYFICSYYQSSICLKNRTSIIIQSLNISVQLPFELDDNSIKGIVYNQESLTYSIFFVDPNRILLQLMLQLGNNESKQFTILTQDILYNVLKQEEIELINKVKIIKEITIFFNQRLFGLIFYNNYCQIIDISEINSTQYFLIDGAYLSNNTYVFLYRNYSIIQMKIIAFNNIQLISEFQCKGDIIQEHQFNLIEIFERVYTQTYDDVLSQLEIIHLEMKGDQFIMKFVMIYMIYFNIIFEISFNNIKYDNFHLLSYKFLRYEQNTHFLDLLYADNNFVMITLSSDSNSFINVYDIKGEMKYKDYLDSIQRIESFQYKKIEKYNASHYVINNHQNNRVTFVTINQLQIECQGQCNEPSNLILSNDVSSISLEIQLNNKSYLNVIITINSRIIMTNLIFILIYIKFGKRAKRNYTKQFYGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PPF0</accession>
    <name>A0A8S1PPF0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1240097</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PPF0"/>
    <dbReference type="OMA" id="GEYDQND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="144"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8104619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="554" mass="66179" checksum="DA0BFDC51BB23AF1" modified="2022-10-12" version="1">MDNQFYNQDSLAFGSPQPISQLQPSKAIKFENVPQKQNKLHSNVKFKEASKKVQQQKIDSVQKIRPSSAQKQEKFKPSWNDKVESKPKQFDNLKKNEIFKIETKLQKQQIEREKENKRRQLENPPPAAVKSYATIKDIEQVINDQQYNKDVSEMKKQKRELEQLQWMVDQEKKLKAEQNKQFEEKLKQLKTQQHPDLAQDIMNAKKKGIENKESPYKTKEQKKQVQKKQLEKNVDDIYIEPQQNNYPIWKDGINLIHYEAKKPLLPGEYDQNDLDLIPQKQPQQQPQQQVRIDDNKLKNELIEIQKRELQRQHELDALIDKHQQLLRSEAQNPNKYYKEINEKLDKIKGKVDPLVKKIDSVQIEYPFTGHLLARGVSKMIQVHSERLTELLIDDLLFEMVDILDTLEMKKKEEERQRYEQMALCDYIEAVKDIALDQDKVEQNFQQTRQQFMRDTRLDQYVPVEQLGRDQQYYVNPMDRLRSIELSSGFIIKIMEDRKKRIQYITQDKQYYPKNLEQFDLITETILEEIIWERAAALYQSSDEMAEQIFINEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQA9</accession>
    <name>A0A8S1PQA9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1270015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQA9"/>
    <dbReference type="OMA" id="SIRENCF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051942">
        <property type="entry name" value="DENN_domain_containing_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288:SF0">
        <property type="entry name" value="UDENN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="cDENN" evidence="2">
        <location>
            <begin position="374"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="13"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8105325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="704" mass="82627" checksum="41BE1418DAF06A41" modified="2022-10-12" version="1">MKSKQYVSQILDLDQQKAQIKLLEEKNEKLIQQNYDLIREITQYQIQLREKDQQIEQLKKENGSLKFELQLVFKQEDLYGVVSLDRYKKVSSAIQPQRKRIFETKKTTLHMNYLNPKKCNNEQEELISPGIVEYGKENDNQQIKAVIEDVQVKEFQQNNLSEIKQIPQIQSKLILEQCKGEESNFTPQQLLQESILIKQDQLSLQQEQKKQYSLFEEFLIISVSKSTLNGLSNNILETREINLKPEILFQHSSAESSYMEQLQLLTQQIHPYGLKVKLEEKTLSMSNLNQILMGGQSYDRLAGAYVITMKSEQTFNNNSFINNASQINPDNISDLISITNIHHQLYCICVDVPDFYDYNVSNNNSKLKKKYFYSLSKTYCFITKAPIIDFFIEIIKIIINTLKYKKAEIYSMVSEFEETLRNIDNSFYTSFKPELFNFLTELQHSKFISECNYSFLQTPIKYQLIHCQDFQNYEIEWAISYALSHMELPHYLILLINMLLEQNICIISSNRTLLSSLLMILPHMIQPFQHIQPTIHALTAQLMPILDSPVPIICGIVKDNENGLHHLGIEDIDSFYDEQSQILFFDANKLKFFNLNNNLTERNIYTTLFYKLFQTYQQIRQQSVPGHYLPDNNEITMNLSIKILDDTKAYIQDTLLSLIPEVDGEINLDQIQEKILNSTEDEIFKQIVQTQYFSYYIQQKFQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PS20</accession>
    <name>A0A8S1PS20_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1300012</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PS20"/>
    <dbReference type="OMA" id="WAINRAH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF50">
        <property type="entry name" value="BIFUNCTIONAL ALBAFLAVENONE MONOOXYGENASE_TERPENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8106220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="58755" checksum="7BD73E8A442AE32D" modified="2022-10-12" version="1">MLIQILLILFGMLFCLFLIKPLVVMMKLKLQFGSACRIQFGIYQILGLHSSSLIEKMRRQSIPKTVNFIVHNFAWTVQILIIDPDYYQYMLINHENYSQINDLNSNQLLDEGIMFQQGEKWKKQRELFIQYFDIEKLKSNLPTINEIIQKHIKQYDQSNDNIKSLILKIISDIYLYSFFGQDANKLQINHKCIGLETIDLFQSLSKLSNKPYYLCKKILFGVQALKMFPNAEEQIIQTKINNIKFSIEKIIQKRIVQHELKLDEDENEISQIPDDFLSLYIQAYLQSKKGNNRISFEEIKQQFFTFFKIGSQTTTQIIQASLHYLAEYPEIQNQLRQEILSFCKLEIIQTQELKSLVHLSAFLNEVLRFINPLPIVRKVQKSHFIKDLKLDRDWIVMIDYSITNLQEKYYDKPKVFDYQRWLQSQSIKENNNYIFIPFSNGPRNCVGSSLVTIIYQIILANILRNFEITNNEISATNKEIAFVQYDNSLKLRPINQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PT58</accession>
    <name>A0A8S1PT58_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1280099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PT58"/>
    <dbReference type="OMA" id="NKVSSYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="152"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8105768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="234" mass="27222" checksum="AEF94E38E7F04490" modified="2022-10-12" version="1">MIPLQNFNHRQSASVLYRQYESRKQSPVTHVVVTNNPRRSHQFDGRSNSPSKILTTSYVYPKIIQSTELKTRIPTQGQSIPIQEALKIYSSKPNTDSQNNSNKVSFYNQEKFNERSNYTKTTTGESQDEEKSSKQKPSIIYQKQLLDKDLQILQLQKEIKQIKESNKKYQNVEKEYDKLVQENNKLKSQIQLQQVQITQLTHQQSLISPGRPSEYVSALSEQDKLKIKTLLECE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q0E1</accession>
    <name>A0A8S1Q0E1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T1360117</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q0E1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000011">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="15">
        <location>
            <begin position="87"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="16">
        <location>
            <begin position="286"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8108308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="55792" checksum="3966BD1B84C4C450" modified="2022-10-12" version="1">MKEIITIQVGQGGIYNYQLNSTQPLFIEIKQSYLYHINISNVDFLDEIFVLVLQVGNACWELFCLEHQIQSNGQVINQQVIEKDDKLRTFFSESDHQKLVPRSLLVDLEPTLIDQVKTGKFKEMFSLEQFVSGKEGTANNFGRGHYSYGKEYVDICLERIRKLADNCSSIQGFMMFNSVGGGTGSGLGTLLLEKLSVDYCKKSRLCINIHPSQETSVSMVEPYNSIFATQSQLEYTDVCITMDNQAIYDICKNKLNIETPKYSNLNRIIAQVISSITCSMRFDGGLFTDLTEFQTRLIPYPQLKFLICSYAPIISRQNIDNVQLSTDEISKLALDTANMMAKCDPRQMEYFSCSILFRGDNIPRDVSHSIYELKKQQTIRFVDWCPSGFQVGVDYRDIYTLPEEDLYNNQRSACAIANTTAMSQFFSNLCQKFDLMFAKRAFVDQFVKEGMEEAQFVEAREILANLQNDYQEACVGIFENDQEENTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1A2</accession>
    <name>A0A8S1Q1A2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1390047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8109025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1A2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="167" mass="20000" checksum="B22E973F72517EB8" modified="2022-10-12" version="1">MGKKKPDSFLSQMSKLFNGEKIIKLTIKRNLKEQYPQLRKRLKRKQKQQLLNEIKTANDVNEIIIRSQTQKLTISTSSNKQQINKFADSLSNIYRLYCHDQQKQNKKKPVLLPTQPNAKSKTQQRLKAKKLRNKERRQKRNELQKLKKKELQRQKTFASKKREDKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1E8</accession>
    <name>A0A8S1Q1E8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Serine/threonine-protein phosphatase T</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T1390055</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:20630"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8109041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1E8"/>
    <dbReference type="OMA" id="CERIMHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07417">
        <property type="entry name" value="MPP_PP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000036">
        <property type="entry name" value="Serine/threonine protein phosphatase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041753">
        <property type="entry name" value="PP5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013235">
        <property type="entry name" value="PPP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051134">
        <property type="entry name" value="PPP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08321">
        <property type="entry name" value="PPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033096">
        <property type="entry name" value="PPPtase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 16" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="16">
        <location>
            <begin position="8"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="16">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="17">
        <location>
            <begin position="186"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8109041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="479" mass="55162" checksum="C3A7568BFB364F40" modified="2022-10-12" version="1">MIPNQHDPEKLKELGNEQFKLSNFQKAIEFYTSAIEKANGNQRLVCLSNRAFAHIKMENYGLAIIDADEILKEDSGFIKAYYRKGSAYLLLGKFDDARKEFKRADQLTQGKDADIQAKLKQIKQAIYEREFAKSIERPDESSEPIDIKDIFVEQGYDGPRIDNDISEVTPEWCEKLMNHYKDQKKLHKKYAVMILQKVGEILKSQQNVVEYDVPKDLEFTVCGDTHGQFFDLLNIFKINGNPSVQRPYLFNGDFVDRGSWSVEVIMTLFAWKVCNPDIMHLTRGNHESRNMNKMYGFEGEVKHKYDDKVYELFQTVFNYLPLAYTLSKQVMVVHGGLFSNDGVTISELQKLNRFRDIPEGGPMADMLWSDPIKQNGRHPSKRGISISFGPDIAHKFLNENGLSLLVRSHEMKDQGYEVEADGRVITIFSAPNYCDQMKNKGAFIIFRGGEMKPKFVQFDAVEHPKLPPMAYARNYGMFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q457</accession>
    <name>A0A8S1Q457_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1420124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000146">
        <property type="protein sequence ID" value="CAD8110017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q457"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="224" mass="26528" checksum="85274AC5D1E26CF5" modified="2022-10-12" version="1">MKGPKYVETNFQLLLQRRLEYNKHINQIKNAKCNPMLEKRSLSQQQSFFKQLEQNWKQANLKREQMKYEDIVKLNTKMLLKVANAKEDQLLSQNSQKGRIDSARSSKSLHSVIKKTKERELFQENKRLKDKIENVQSSIAYNKIIENFNKHQGERDRLIRNSYKKQKKIKDMLDNLTSQSKASLHSKINQVPKLRLPKINQQGESIKSLQQLNKLRIDGFYTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7M8</accession>
    <name>A0A8S1Q7M8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1480023</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7M8"/>
    <dbReference type="OMA" id="DQFWISH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8111528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="648" mass="74676" checksum="5F0F51176250BF66" modified="2022-10-12" version="1">MIEDEDSTFRIIVILLNSLSLTFTILILLFICRIKQLHERQGHTIQIVLILECMYSFFLILSQFYFDQPDVYETTFLVNTNGKGCHAIGILNVFTSFAYFTNINLYSLESLGILSSRFTKNYKLRHLAVVSASTFFALLALLSNAIGFNQFGMCSLLSEGLFTVFLLILTSVTFIIIIWTCTQIHRLRKVLGKDLTSKIQDKMYKVNLVYIITFLIIRILPVLILTIITLADNDNETYSDAFYALAVPMSLGGVCLSLIRSQEPTIQDYFNKKLQRQKSTIVELSIPQVEVRALSNSNSNLSTESQIIKTHYLSYLQGTTRDNSVAYRKTCLDNEELIAKLTSQMENNQIKLKLKEFLFVFKIIREMNIDVIIPTINTNLTHYHMKHMRQRIFYFDNDASFNASEIICTSYSESVFLQIQSIDSLMKSFDEESNKVFIESLQNSRLKLQTNILTTYDGEVLISIINNQAKRYLTRHLLSAIYNLNIQVQRSFIPPMIGLYSIVDEINQYTILVERNLLNLIMLEQQGFKIQGFFTYQVGQVDEEIKGGNDIYTKIKMNPKVKQEFLNTLNQDLSILRDQFLWGYSFNVIYLEAEERLNTLPCMYKTRNGYVIASLGGVLDQFWISHNEASAHPELYASQLAQQIDLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7N7</accession>
    <name>A0A8S1Q7N7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1450074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7N7"/>
    <dbReference type="OMA" id="KYIKSYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="170" mass="20208" checksum="DA6ACA2DEBB32BDB" modified="2022-10-12" version="1">MHQNSGLTYHNVDSYIETEGFFCFEQFTRLDLKKKNWEESLNSLTDDLIALKSEYVDLENLEIFIALLEFLEKNIQESEVGSPYYQLRQGMNQTSINNYINDMLENIEKSLTCDSDDYRAIDFPGLLKLEDILSFLKESKYIKSYMQKVDYLLQIQQEKKQQQQQIKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBZ8</accession>
    <name>A0A8S1QBZ8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1530099</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBZ8"/>
    <dbReference type="OMA" id="KEMRYQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="21"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8112973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="585" mass="69030" checksum="58314CE22FE4642E" modified="2022-10-12" version="1">MLQLQQPPKIQNYPGKRVLNYTFLKELGQGSFGQVFSAKVDKTQDLVAIKCVSKAKLSESRGIVGQLLESEIAVLRQINSEHVIKFIEFFQSENQCYIVLEFCNSGDFEQLWNRRGKKIPENEVIAYMKQVLAGMQALHEKNILHRDIKLPNILIHNSTLKIADLGFCKQLKDQNQHENLILGSLGNMAPEIIEQKPYGMAADMFSIGSMFYQLLFGCFPFTNINEKAFLQDIRTNKPNFKRNGVSISTQLEWLLNKMLMKDPRDRLKWSELYSHPLMKQKEMRYQQISLNLLQAEQINVEKIGKFYQNQVQQDTFENPDDFLKQLDNMKEQHILKQNKQVNIKETELLPDLQDEQVKLEISQTEKQIEDLKKVEELIEKYMRLRDQIVYLSRTLNEINTLISEKYSAFPFLLLIKKIYLLNDSLTKALLDKQNLFKVADLLEKVYQHPYFQKFISQMLDDQQFYESLLQFALKSAKSFWDEIEDKNWQKEMTIDFSSQFNQNFKEILSDFIIRTLSEKKEKEKDPKVIEQYIELQIHLIDCCCYDKVYQNDNKINITEQFDALYEKPLKIKNDIFSEKVQLLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QC97</accession>
    <name>A0A8S1QC97_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 10">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="1 10">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T1520046</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="1 10"/>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QC97"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07852">
        <property type="entry name" value="STKc_MAPK15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000238">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000166">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="13"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="405"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="471"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="410"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="428"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="471"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="481"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="499"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8112597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="510" mass="58693" checksum="410F5719E50D1C6F" modified="2022-10-12" version="1">MSEEIEPHIARKFEIIQKLGKGAYGIVWKAVDKKLKQVVALKKVFDAFHNATDAQRTFREVMFLQELNGHENIVRLLNIIKAENNKDLYLVFDYMETDLHAVIRANILEEIHKKYIVYQTLKALKFIHSGELIHRDLKPSNILLDSECLIKVADFGLARSLAQTEDDSQIVLTEYVATRWYRAPEILLGSTKYSKAVDMWSVGCIIGELIVGKAIFPGTSTLNQIERIIELLGKPRGDEIESLDSPLAANILASINTSKKKAFQSFFQGASEEALDLIRRLLCYNPNQRLTAEQALKHKYVRDFSSPDEEIVCQHPLRITMNDNKKFTIKEYREALYADISQRKKEQRKKWQAKYLAQLGVSLDEKQNQVQQIPEKEETIQEQKLSQPDLIQQQIYQQQLLLQQQRKAQRPQSGQMGGRQSSVEMPKQSQAQQQAIVPQQQTHHKSSSMIQSMQTKQGYYYPFIQQQMAAQPNKVNKSAVQTQQQQPQQRPSTSYYQKPLTQVTTSSVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDK0</accession>
    <name>A0A8S1QDK0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1540088</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDK0"/>
    <dbReference type="OMA" id="KNCQLEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15505">
        <property type="entry name" value="PHD_ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000750">
        <property type="entry name" value="Chromatin modification-related protein YNG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF42">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="162"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8113204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="214" mass="25148" checksum="B3A5EA858B36FB57" modified="2022-10-12" version="1">MSNIEEFIDLISYAPIDVRRAFSKVRELANLEKKYEESLVSTQKLVFQLKSVESTAEKEKFILQLKEQIQRNHLQLLALHRERIAALEIIQQNYQKYIATLDIEGEKFLEELKQLPREQEDISRKSQRDVKVEPPVMVQKSDKIKKTKRPLQDEQVEDAQQHTYCICQQPSYGTMVACDSKNCQIEWFHLVCVGLSDVPDEKTKWFCPNCRSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QEX8</accession>
    <name>A0A8S1QEX8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1600035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QEX8"/>
    <dbReference type="OMA" id="ANSQSNW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="1">
        <location>
            <begin position="4"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="197" mass="23540" checksum="D6A4856F9C957E98" modified="2022-10-12" version="1">MPFVEFNLDIILKYGQALYLANSQSNWVVDRMCRMTCIQDSHWQISYEVFQNHFEYKYAIGDYCLQNQSYIQWEKGPNRNLNLKNDMAMTIKNKWEQRKIQFLLQTDSSASVVILMSNLDSIQKKLKNNKDMKNNQKIFHMSHYIDINKIIMGIEVQYYLLTKGKNPYEFISKPFTLNLDDEQCFQTKDLQFTKILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QY70</accession>
    <name>A0A8S1QY70_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T3780003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000387">
        <property type="protein sequence ID" value="CAD8119857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QY70"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8119857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="163" mass="19172" checksum="07B6ABD25B5D350E" modified="2022-10-12" version="1">MVPLFQIIAVQFIQIQSLLEFPSSRNVQNIKIKSIFLLTILSRIIIIKQNELINEIKNDCVKELSCDPINTIRNNNFLATITPSCLRLFLFIISIYESFYEKIQEINIKIQVIKEYANYFLFLNRDISKSQKEDSNYLLIQHILGLQMIIFNQQSSLIYSIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JM10</accession>
    <name>A0A8S1JM10_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0040264</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JM10"/>
    <dbReference type="OMA" id="NILWMSY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894:SF5">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE RSMI, CONSERVED SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="1096"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="442"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="660"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="709"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="803"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="303"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="453"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="709"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="756"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8043044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1279" mass="151810" checksum="4C12EEAC14DB78FE" modified="2022-10-12" version="1">MNNTNCFPKIQKAQSTAKLLIKTDWDQSINSSIPLTIRAEMSSNRMKTDFTNKNFQTNSDVSYLKNKHKKFQSIESSIYKDPELSQTKYDKSLQSFYSTMYRKSQNKLQFKNKIDKNINKVQWLDFEELLKDKNLMENGLQKYFFDILKHEIKQSPNQSVIPPNRQQALDLKEWLFFMLQRTKDKSKNLTSIQIAEEIQLIYTSCFKEIIKQVKVECVDRGELLQIIWDFYIQLIDQIIKSSNQQMQCVEQEMKNKMIEQQNFYNEQLHYQELIYNESQNQIKQLQFDYDRALDIDQQQKQEILQLTQEYREALEVIEQHRKQIDELQLQIVMLKTEQINENNPENEKINIKVQQGQSIVSHLMNFQAKIDNNQKYRIKSPFKSQGSKSPGRSPKKNSIMFDRWEQLRIQENQLKSQIISLKYLDFDKDQQPSQNKIIQISGTDDKSVQTEDQSQSQNQNNNQQEQHNLHSQQNISILEKSKQVSNSLEQKISMAMKILEEQKLDENNKQLLCRTLSESKEVCEQNKSLSMRLISMNRLDSIQKMELLEKEELCQELEMKLQQTVQDFISIIQNSKKKLYKLVTKNTQLIGTLKQIESKYEIKINEVIDEEECQINNYLNDIDQNREDNELSLSKESQNLDDERYPQEKLIKMVDSINLKKQTNQKSQNYQEDNSETNNNDQSEQIDNYFDNQTSPKDIRYLQDIYHSQKESEPNDENTFKDSPDQMPSDHSKSDLLINPNQNNKSKELDENLIQKNNDLKTSTKNKTNSKNNPTNSKSIQRIQQQQLGDSKIINQQQYHIRNSKNQLDNNSIKPTKTNSKHNISNSPIRETDEISQINQSQRSLVESDRIDLDDEKNRSQRIKDVISQYKDKQFTKYASAHKTLNFNLLQETKQAKKFRRVYSQNTDVANNLLKQVINSKNLTKTLSFLQFNKIINQILTDVSKQNEAYKIPFHVCIYDFIKNKYGFKQVAEKKIKQIYEFIIIEKEKNAKVLLISKYCNLKNEIDEVAQKLLIEAFLFFNNKIESNKTEFFITYESANEFLNEKSQLWLQTNQMQQLQNQFKVLNQQYGSNKHYINFDQFIMKILDFYIFNKKDYQKILETLFKAADLDGNKLIEYQEFKTLYRAIHTQQMLTESLLQVFLKNADFADDQGDKYLTLPRFTEMAIELSIFPKESVIKYGEGCEKIKEHWENEKNTIKSRFLEAKQYLKVKHTFVELDNLINNNKKEIQNILWMSYKLLNEQSLRVYLKYQTKQCLSELLPLEILEIQQQYKQLDTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMX6</accession>
    <name>A0A8S1JMX6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMX6"/>
    <dbReference type="OMA" id="MSLFTKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="262" mass="31534" checksum="D31AA8D12C492406" modified="2022-10-12" version="1">MNKSLLQSPIKPQHRKIISQQQSNRPFRLFDKMSLFTKHRIPLVEEIPQLSERQSRRFKKYFSTTIRNSSCNCINCGKNNLFIKKTFQLFPFESNFNRRKRLYKKFRKYIHCVLFMIRYKIVQKIRDRQRNKMKKAINQNVHIHKPSAVANLLAEGIKQLKQQQQHHIEVLHEHDSDEEFYQFKPKMHQNRKSFAHFLSSPKGQKKLERVYYVKQVNRPISQYIANRPKPNTTQCSPKSILPQLSSLYDFKNSISVRQLKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN27</accession>
    <name>A0A8S1JN27_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JN27"/>
    <dbReference type="OMA" id="QYPNHNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8042957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="173" mass="20227" checksum="F78BFA2C09F73293" modified="2022-10-12" version="1">MFIVNKTTNTNPQSQPDLQEYQNSFNNEDPRRNQSVTLYDRVKKVNSNQNPKQQLSQQLSNLSLTKTTYNSFKTLYQFTQKTITQIKSKQEITISQNFVSSMKESNQFMYKVAKKMSSFIKTNKLVVVPPQKIIGNWVYQLKSKCLKKKTPQNSQYPNHNTGFRDEDDDLIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNL1</accession>
    <name>A0A8S1JNL1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0050214</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNL1"/>
    <dbReference type="OMA" id="QLPMKKW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17953">
        <property type="entry name" value="DEADc_DDX46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22387">
        <property type="entry name" value="KH-I_DDX46_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="10">
        <location>
            <begin position="505"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="537"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="726"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="172"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="260"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="348"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="505"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="191"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8043561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1159" mass="135085" checksum="ED9ED3DFF74D3B37" modified="2022-10-12" version="1">MGKDKKKSKSRSRSNKKKEKKEKKEKKSKAKKDSIRKESIKKKESIKKESQKKNKKQKESKKKDKKSKKKSQSPSPINAQKKQAQDEQKLKDDEQKRLSRLMKAKLIKFQNSDDDDDDDKKEFVYKDPEVIEPILPPDEQLIYDWSQRNQEKQIQDALKKCRLVGEKLFYDSDGIEEDDNPEEQFQDENGNDNKQDVKIQEEDEHEVQKPVEKVDKQQQERFLIDKKTLNNDSDSDSEDENASKVKVFTKNLQSISKLKELQQQQTQQQFSRPQIKRKWREEQYDDQEEDPLEAFMRQVETEATQQADEDERRRKEEEEKEKKRKQAIEEELRKLNAQKVITYEDIIQNSINQNNEKKNTNGMDIEQVQEVQNQQKEELDQQINEEDEEDNKFYEQFMKELKKKELEEKMRQREDEDEAEDVDLEQQQMLALLEEEPDIFDKQKKLEKKELKPVDHSTIDYQPFRKDFYREVSELVQMTAEEAKKLRQQLGDIKVRGKDVPKPIQNWYQCGLNDRILNVLIEKKKFLNPFPIQAQAIPCIMSGRDFIGIAETGSGKTLAYLLPLLRHVLDQPALKDGDGPIAIIMAPTRELAHQIYVNCRWFTSILNLNVVCCVGGAGIAGQLSDLKRGTEIVVCTPGRMIDVLTTSNGKITNLRRVTYVVIDEADRMFDLGFEPQICKIIQNIRPDRQLVMFSATFPKNVEQLAKRVLRKPIECIVGGRGQAGGNIEQIIEFMDESDKLYKLLLLFQEWYTKGSILIFVEKQTEADDLFKELLKYGYKSFVLHGGMDPQDREFTIHDFKKGIRTIMVATSVLARGLDIKHICLVINFSCPNHMEDYIHRIGRTGRAGQKGTAITFFTPQDEHLANDLVYLLEKSEQQLPEKLKEYQKSFMEKVKAGEAKIYRNKNRAGGGFTFGPEEEQKFQDFRTQMRKKFGLEGLMMDEQSSDDEKVLEEIAKGKLSEEERLKKQEERDRVERERIMQLIKDPALKSQILSEATKAANLCINSGGSREQVANAAMDAIKRVLKQHSQVNKSIEGGIEEAMQIINEFEERERNNHDFVSYDFEINDYPTQARLKILSKEFLNMIHELTNCQISQRGSLVEPGKKPLPGQKKLFLRIEGENEYFVMSAYKEIKRMAQDLTLNQIQDNYAGIQTNSKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPJ3</accession>
    <name>A0A8S1JPJ3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPJ3"/>
    <dbReference type="OMA" id="HFETHTL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="119" mass="14053" checksum="058791AA0A6D01C0" modified="2022-10-12" version="1">MKNSKLLVDQKDKKMVKSVNLSLYTIPGSKKHQNLQKSKDEYYMQISPHFETHTLTFTNKIKKVKCIQHEDMDTEESVKTSESPKTDRSQKTSQKTHIEQPKSLFYNEMKCSRRLKTEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQH3</accession>
    <name>A0A8S1JQH3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQH3"/>
    <dbReference type="OMA" id="IVKMKEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14014">
        <property type="entry name" value="STKc_PknB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="96"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8042820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="369" mass="43900" checksum="0A38D578117A8319" modified="2022-10-12" version="1">MICIKKNLLFHDSFQVEFLSDRIRLSNPKKMVQHTIKLHGNSIIEWKLSKCQSKLSGFGILVNGQLEYFEMDKIHLELFKQFLDAKISYQNIHKIYQMISFCGRGSYGHVFKYQNRLNGEYVACKSLKIGRTNNYEDFMIEVKALQTLKHPKIVKMKEYYVESKHFYIVMEYLEGKSLRELLRQRILKDQEIILILKQLLQCINYIHKEGYVYRDLKQENVLFAESDNLETLKLIDFGIAVIKTDLIKQTNRVCGTPGYMAPEMMKTQSLYDERIDIFGLGVLLWEMIHNCRFFEGTNLEDVYRLNEEYEFCIEYTQNIENPILRYLVERMLQHNPEQRITIIDALNYLNRVDINSCEYIKDYDNISTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JS84</accession>
    <name>A0A8S1JS84_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JS84"/>
    <dbReference type="OMA" id="FYFNAQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="172" mass="20435" checksum="BE06C8C3B84E5470" modified="2022-10-12" version="1">MIIVNNTPKRQKTQDSDLIDSLIIVEDSNFKENQVEYQNNLKLRNVELQQLRQKNTKNQTLLISTQSQSLQQQQICSKRGSLADLHSNLPFSQTYLGQMQNRKKREPTNLRLNELQQIRRYESLENLIQQPTNRNYLKTQNDYTSRNKDNFYFNAQQVQIQQLIKTLGSKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JT60</accession>
    <name>A0A8S1JT60_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0040560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JT60"/>
    <dbReference type="OMA" id="SMSKNCV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="2">
        <location>
            <begin position="286"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="2">
        <location>
            <begin position="670"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="2">
        <location>
            <begin position="839"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="951" mass="110897" checksum="F1D19EA37803BCB7" modified="2022-10-12" version="1">MEQEIIDWANTFENTKSIDDLKTGVALCQIISKELFQDKRQCELKIRVINCTKQDYQSCIRNLTFALSLLEESQQISVKHLTGQMLYQNPLKIFQILQQFYRSKSVSSASSKPPKAPQKQDDPLFYVDENLVQPNQSIQITSNEEIIANKYKMIKPNSPYQETNNYQQRSQTHQTEYAETSNRSKPQNQSNDSISNLTPIKSNKESYNQKLKNELQVLENQIVKEQQNLQQKQKRENKSKHSQESYSQHEQVNQDYIYNQQQQQQQLQQQQQQQQQQSQQQNSITIEQKQQLIEWLKQIRLIKSNAQGLENKLPKICKNGVIFFDLINRLTGRDEVLKGALRNPKSIREIRHNYRRVLEYLKQLERMSYKYLNQEQQLVDGDEETFWGLMHDIKVYYTNCGNVAIANHSQSVDTTLRQTQKQQPIQQNTQSFQQHSQSKLQQQITLSQKKQQHKQSFADTQLEITIDQPQQSKSVKPSTANRSPVGPYSRIHNMKQEYDSVNRSLNSLMKKSNIGSQNGTPVNQSLQHSRNFSSSKQQQKARSTSMSKNCVTREMELYVQTYFKQKGLYNNDNLFTDPIRNGNYILQLLEQPITNNNFKSILEVEFNVDSALQATKAIVDAGNLPAWIGKINKSSIMQLDKSAMGLYWWLLKQQTKKIEPITNSFTLPYRQEEIDQLKLITLHFAREYTSTIQNFNDLIFQCQTGILLCQVETEIFQQKITPIYTKPIGEKQCIANIRKALDYLKGKPIGQRFVWSEKLIYEGDQLIILGLFEDLRRYFDGLPQRQGDSYFEDGPYLKKSIVQSKGISKIGNLIQQEPFSLIQKESSFAQPQQVSKKLIFDDPPQFEWLNNFGFKINWNNDILEEFKDGIIICSIVQKLENIQFKGLQYKPNTNAGCLVNIRKAFSVLKDKLDMPLELVIEQEKLLKGDKEYIIKLLTAFKNIYKNKLKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JUK2</accession>
    <name>A0A8S1JUK2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100279</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JUK2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="183" mass="21682" checksum="1CDE2DD15AD26736" modified="2022-10-12" version="1">MKIYEACNSSSYYTRIKETFLADSGITTFMITESATMILFTRELRGTTWCFPITHEVQMSIFCQQFCQFMFSLELKRGEYNLNWSSQQSTLNDIDCYLIINQNFQIFLLKKLLQENFTEDWPKLVKSENKHQKLKSKLVDTNSKRVELIKEFVSTEELEQLLLEQYINKEKRTKLASSKKGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWN4</accession>
    <name>A0A8S1JWN4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWN4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="419"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="456"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="502"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="540"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="606"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="670"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="782" mass="93208" checksum="9035AE0B8C02E534" modified="2022-10-12" version="1">MTKIKGNPNFFEVYIIDKTQNNFQKQFPLDYLLVINNNNYSIFLLLFQLNFPAKSYIINFNCFFSWIYFDQYITKILIILDKYILYFLKEIYFLMDLYEEEPEFIRNVNLLKSPPNFQQAENHSKAQIIHKEVINDSQRCSCCNLHINSIKYPLLTDLNEFMVHGTAYKFFLFIKINFILLSIVFIIAGIYNLIQNFKGDQCIKHEICQQNLYNLLSIFNRLDSEFDDYILDTLYFISILIQLAFIRYISCYQNVNYNLDQEIDSELTISECSVQIQDVNTQWDKTTIMDYLVNYQSKNNEKFNIIDCCLIYNQEHFENQLKVKIREILIQNNDISLKNVINRIVDQIYILHNREQFLKFNGEVLITLSYEVIIILFIFKKEAINFKKAIGIDYKVVKCPRPTDVNWSPNFQVYNMKRFCIKYLIGFCVLIIVIPIQQAKSEYIIKNHGKSGNQLYFNILLSLSIGVLLMVLRKIYYYLMLSNFQDMMQKVNNKRIKPTLESIQYNIILLFQFIVSLSGIKFDEDDVEKRKTMIWCSGGFSQDIINIILINAFSIIILSIIDDVQVWKFIKIIYYKYFNKNSTLTQFEANQLMAKKMNFNQKKFDLILLIGLCSFCGYMYPIFYLITLITLFIIYWINKYQVINYGVEEEFKFEFYVCQKFHHILNHHSISQLSFCSYQLSFLTITLIGFFKSKPDIIKRMKIQQKAFNNDLYSKCNPLIDESLLLNEQYEKWEQQYVEGLQLNKSSRQAKFYSALQLKLLREFEREFQQSSIQKVQILPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXT3</accession>
    <name>A0A8S1JXT3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine Rich Repeat family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXT3"/>
    <dbReference type="OMA" id="CLRLGQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="651"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="651"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="672"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="695"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="707" mass="81185" checksum="56BB4A29B5C0E3AD" modified="2022-10-12" version="1">MRPQTAKPKLATNEYTKEIEFLKQQKLIAYGGDPSNLPQIFQKNPAFLVLDELQAELFNARCHDTGETVSIERAKIFKEEFNKINSKKFPDVLNLYSMRLGVTTLIALANQKPIQTTINLADNTLGDYAVHAIKTLLANSQIKNLNLASNMISHIGLGQIIDDLCKNQTLKSLDLGVLDGSIRKNALGFEGAKHIAQLLLSNKKIETLKLQDNVITTAGGEFIGQALKTNQSIKHLKIAENDLKIAGIETILINGQNLESLDLSKNNIRPTIKFQEYLMNNRTLKRLNLEFNDLGPKGVEYLANGIIQQQSGLISLNLRGNQIRDEGLQVLSAAIYESESLQELDISLNDITPDGIRYLADVLPNSQIKILNLSKNLLGDESMIMLCNNRIMEKLDVSSSRVADQGFMTFITFIGESTNFTHLKANDNYISEKVEKLILEIIEKNQAIIDLQVTGNRLSLCCLNRIQKILHRNQKILEEKEPSKIRTEIYRLKYEQKKIQIAKDKLSQQEKEILQIEDKKSQVIVEMERIKQQEQSKRAGLQERITDLLNQIEKKKKIIEDKQADFERAKQLKQQELDTQKNDYQVVYQKRKELEQEYERLQKELDTQEQKFKDEQQKLEDAIAEKKAKGEEIEKQTAQLREELMKLQKQQFEQNNQAEKEQQIQQQQQVINEKEEKAENEKQPDQQDQNAEKLKPKKSTKKGKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1B2</accession>
    <name>A0A8S1K1B2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1B2"/>
    <dbReference type="OMA" id="ICDDHAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="255" mass="29890" checksum="DF0366E7662E863D" modified="2022-10-12" version="1">MGSNCSFNQQGSERSMMMRQDSFFENGRIAKPQLDSMIGASQLTQMEIPNAQLRSPTKPSISKQNEIKSLVRVPSEESLFQKEFCQIKESKHQNNLKSEKTIQPKLQINLLNQKQRYHQQNQQQSPKFQSATKKKKLQEKSQSHSPHQNQYIPQEKSKKKRESLKNTHRNLEKIVPKRKYSDLPEKKEKVQQIRRKISDICDDHAYIIGLDSPTKFRSLTPNSILKRKESEIETNSPQKKQVRFKDQRQKNLICT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2J0</accession>
    <name>A0A8S1K2J0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pectinacetylesterase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2J0"/>
    <dbReference type="OMA" id="IKCIKDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562">
        <property type="entry name" value="NOTUM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562:SF67">
        <property type="entry name" value="PECTIN ACETYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035773328" description="Pectinacetylesterase family protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="47379" checksum="3346E04A502D237A" modified="2022-10-12" version="1">MLIILFQLLWCCFQLMLQFVEDDRAKCLDGTLGSYYFQEGFEGGQNNFIVFFEGGELILGNTEEEFLMNAVEKMQTQQGSSLNRASSLEQDGILSSDKQKNYYFHNWNLIHINYCDGVGFQGYKSESVYFQKHTMYFRGELIIRSIFDHFKTKFQKAELIILSGCSIGGIAALQWEQYLTSLVPNNIEILFVADSSILFDMQTIDGINLLQQSLKIMNYIANNETQNPHKKCAFNFPNQNWKCLYFQNLLLYIQRPVFIIQPFYDIKFLQNYLDIKCIKDQTLKNCQNNEMNLIDLVYLKFNQIIQESLIKNYNTGSFAPSCISHCFFVSQLSFSLKWTIPEGSNRTVYQTLVKWVKRQQSNQYNQGDFGLIDQVPWPMNSACAELSSLINKIKLINMILLIIIIFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3A0</accession>
    <name>A0A8S1K3A0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3A0"/>
    <dbReference type="OMA" id="DCCTINQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035903813" evidence="1">
        <location>
            <begin position="18"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="173" mass="20463" checksum="A693CCE7BE175314" modified="2022-10-12" version="1">MIFKVLLLILTIANCCTLNQIQKKTRILRGNYKDVLNFQQINLISRYNTYFNGGQMNSQLNRDAIYYVNSAIGYTYCSLHNSIEITLFKNYELNTVVVEIWDRLVDVHYDFEIYISFLEQEKKIYENASFAGVIKIRFKDQLVDKIRIYNRGGSNYNGFLSIIKAQAFYHRYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3D3</accession>
    <name>A0A8S1K3D3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3D3"/>
    <dbReference type="OMA" id="CAIRIQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="232" mass="28442" checksum="1B8FE3049FA5CA27" modified="2022-10-12" version="1">MQYQQLIQNNFQSIIDLYYHQAQQSGENRFREIKASTKIQAWHKMHKLRVKYIKIRYSTVIIQKFARGYIARMLMKRNNDSRYNERNIKYFSYHATQIQRHFRGYHYRKYYINWSTRKAYLEFLKTKNQDFLEELKKVEVDENQQLKIRQEQLARTEFESLAKNLHHLSSTQTISGVYNRPFGNKDIVFDMDVESHLKVVFHSNYEWEKKRQMSRYAKSNKLNYSNKLKPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4C7</accession>
    <name>A0A8S1K4C7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0120041</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FYEDAQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000029">
        <property type="entry name" value="Kinesin-like protein KIF17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF21">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="11">
        <location>
            <begin position="7"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="93"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8047730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="386" mass="43691" checksum="4A25CA64CFC0F590" modified="2022-10-12" version="1">MSKKFECVRVVIRCRPLNETEKKDGHVCIVNMDTKNGQVTVRNPKVVDEVPKQFTFDQIFDTQSQQENVYNSTASPIVESVLEGYNGTIFAYGQTGTGKTHTMEGKDDPPTSRGIIPRTFDHIFQRIENMAKNKQFLVKVSFLELYNEEIRDLLSKNIKNKLDIRENPETGIYIKDLSKFMIENPQEMREKLLHGRENRAVGATAMNQDSSRSHSLFQITVETNEIVQGQSHVTVGKLNLVDLAGSERQSKTHATGDRLKEAININQSLTTLGNVISALVDNKSQHIPYRDSKLTRLLQDSLGGNTKTVMIANIGPADYNFDETISTLRYAHRAKQIKNEPKINEDPKDAQIRQFQEEIMKLKQQLELSIEGGGGVMNPXLIIQLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K517</accession>
    <name>A0A8S1K517_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0140166</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K517"/>
    <dbReference type="OMA" id="MCLVEED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20507">
        <property type="entry name" value="CYCLIN_CCNB1-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000001">
        <property type="entry name" value="G2/mitotic-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="119"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="6">
        <location>
            <begin position="212"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="216"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8049791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="336" mass="40148" checksum="9961E7E7DE1C4B90" modified="2022-10-12" version="1">MIHDQYRFFGKELVNSSGEFQKENTDRNKPKHLTVLPRYLKLNLWQEERENKMEVENNTQQLCSFDQQMIKDPQYISLYNQEIFTYLLSQEEKYLVSNNYMNEQQQPDLNARMRAILLDWLIDVHLKFKLREETLYVTTYLIDRFLNLKTTTRQQLQLVGVASLFIACKYEEIYPPDLKDFVYITDNAYTKQDVLDMEGQILQTLDFSITQPSSYCFLQRFGRIAGLDTKNLCLAQYLLELSMIDMKFMNYKPSFLSAAAIYLVHKIRKTTQSWNEEMQKMTGYNEQELRYCAKEMCLVLQSQEKSNLQAVRKKFSQPKYLEVSRIRVERQVKQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K5C9</accession>
    <name>A0A8S1K5C9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090484</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K5C9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="219" mass="25661" checksum="BD012077D0579C66" modified="2022-10-12" version="1">MCYLYENVLMQLHHVLHLREIKQLVLNSWVLQFEIIVGILQLHWLQLIVQKRSAVIFQVKTIKYVMMICHYLKLLMNPFCVFDGTSCIDYGKNCNAFNEPKETCPTYLGKDGPCKATTVGTLKGSCAKRVCTEAPNTLTTDDDYQKYHKDCYTTDYGCTNIISCLQIKRGMDMGQLMYQCYYCMFYLQQHQLFIIYKFQSEWQVLCLVRIQLNLQSLSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K709</accession>
    <name>A0A8S1K709_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0170137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K709"/>
    <dbReference type="OMA" id="NDWESWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8050597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="281" mass="32565" checksum="7FBF25DF2672A753" modified="2022-10-12" version="1">MKVFNNPHFHSKGSSSKPSSLQSSRVGGSKIIGSPQGQEQDLNEMLQSLNPTQIIAQEEESKFDLIQEMQQLKTKVLGLLKKYQVMLQNENAKQVKSKIKSDVQEIQIEYEDIIAQYQIDQSQLEIENNIPTVQKEKELNTYFNLVEEFLERFSHHKATLNYFSILQQEKMNRNGLGVFSVDQDGRQSMYDFQEQLPDLTDSNNSENTHEAQQAKIALSNSQKLRSQIRRRFFDLALKVKEGLPLYHPGRQILVSILFEDAEEEGIDENEWESWIVKQLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KA46</accession>
    <name>A0A8S1KA46_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0110434</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KA46"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="60" mass="7286" checksum="5B59546DFA9F770C" modified="2022-10-12" version="1">MEIQMEEMQKHNLYMTFEHLKDHLSSHWGKWCEKIEKIQNYTIDTTGSCTRKSKRVQLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHY1</accession>
    <name>A0A8S1KHY1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0230007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3244"/>
            <end position="3268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3280"/>
            <end position="3304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3316"/>
            <end position="3343"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="271"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="316"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="374"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="426"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="481"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="578"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="625"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="772"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="825"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="880"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="927"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="979"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1023"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1064"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1122"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1168"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1217"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1265"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1312"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1359"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1400"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1456"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1506"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1544"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1594"/>
            <end position="1631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8055100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3382" mass="388261" checksum="B5D814DF1D132453" modified="2022-10-12" version="1">MAGLHPYKCVSYAFICKGVTEVQVKTGEDGTQHQCHPKFQPWGAGTPTLDAYMVHCGFHVGVSLVIEQLPDESYKYYCKYFADNSHNKCLITAYQKGLFRCQMCKLPFFGNGFGCDQVIPRYDIRFMYPNLRVCNGCCDICYEDGECMYCMRGCGEKDNKNNKQCFCSCSGHYYCYIDYRNSRFIEYPGCLEGYMQIGSSCFGISPCLYAIPSAKNIYNCQSCQTGYQKQVYSGSDYRCYMKDWNCDPSRQSMIFDYQIDNKMYYYTTCTLCDPNFFYSKKHKQCITCYSKLNQIADNGICKCKPFYGLLEEICTLCTDGYCHNCESDDFYQCTSCKPGSNRILINKDCICQPGTYDPENDDQICLVCDKSCQNCLGPLFQQCIDCVEESISNRIQIGNVCQCKTGYSEYEVREQTCGKCHAKCLTCFQPADASENQYCLTCIPGQNRVVSDTFKCDCEVNYSDFGGILEVCAVCHYTCGNCIGIEAINCSQCSETSNRELTFLGECLCKQSYFDDNTQNIKCQKCHHSCLVCANSTEKDACLECPSSRTAQSSGSFFECFCTDSKTFDDGFSLQCQQCDQSCKTCFGPLSANCLTCDINYRQLELSFCVCPINYYDIGQLECAECHYSCHRCFDNNEESCIMCSFDLNLRILKGNVCKCVDGYYEEVGIAKCQKCAYKCETCVIQPDKCLSCPLNSMRKLDPIKGCYCSDEYFDKENEITCLKCHFKCQTCQGIEQNDCLSCNSNQHRELKNNLCQCQPNYFEMEVQECSACSPFCYECINNSKNCTSCNNDRYLDGNTCKCKTKFHGVAISTFDFNGMLTCQKCHYSCSTCIGMDEGNCVSCIDTENRVQVGNTCVCKEGYFDTGLPTCAKCSYKCKGCQKYFDRCISCQDNSLRLLVSGFNKCQCIQGYYDNGQNNVCQKCHFSCLKCNDIETKCELCSNESNRIYNDQLFSCDCNIGYYDIGVENCQKCHYSCLSCNQGGANSCISCIDVNTTNRVFYNNTCKCLFGYFDDGSSISCQKCDIQCLNCIGQSYQCLSCPQTRKIETNCKCHQGYYDIGLQFCSKCNTQCLQCETTSNNCTSCDSNQFREINIINRTCDCQIGYIEIDGICQQCNSSCKTCSQSLNLCTSCIQFKNLKNNDCICNDGMYESTIDKSCKLCDKTCLTCANTNNYCLTCSIDNYRQLKNGNTCECIQGYFENPINQDCEQCMSSCLTCFLVYDNCLTCDNNLNLSLVNNKCLCSQSYFFDSVNHQCQQCNITCLECQNYNECTQCRLSTRHLVVDLNKCLCNDGYFETNQQHCQQCHFSCNTCENIYTNCLSCMSQYQRILKNNKCLCLDGYYEAGIELCYKCNDLCKTCQSFATFCLSCYDIEQNRYHSGDKCICKPGYYEQNTLICSKCSNECLTCQGSAGYCTSCDTNSKRVDQSIIHKCPCIIGFYQDQNFICQKCDIKCQTCLNQSNQCLSCTLQLNSNRKSLSDQCNCKDGYFDDGTQIQCQKCNLQCKTCEIKANNCLICQNSMRINTPTCNCIDGYYEDEQLNCQICASKCNTCLQESSNCLTCQPGRIEKDCKCIDGYFETGQKLCQQCAFQCATCVQDPFNCKTCKGNRLQEPLCTCESGYYDDQLNEDCQKCDSTCLQCNMNGCLSCFANRELNEEMNCIPPPNSIWYDDTPWCSTCQVAVVNSYLSDDLSMIIIHFDFPLNPKSFDSYLEVNKCSQLFELESVQNFGQNSVCTINSENNKELFIFLGENSKMNVGEAIKFKDNSLSHVNCETTLKTFIFATLQQPKNPLPPKINYHVPLHKLNPFAENQVYLQSIKNNGNRKLINIDWSCQVESGESSSMLNQFLDQINFLQEQNLLIPKLTLPSDAVLKFRIQYQNFILISSYSEFTIYTHAGDIPQISIDIKPSYFLYETINIGVSVGTLVNTISNESSRYLIQLIEIDRYPQKSSSSNLNTTIDSNSFEMQKFNLYILSHCNQFKFQHIQQFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXESSRYLIQLIEIDRYPQKSSSSNLNTTIDSNSFEMVYTTIPRYTLSKNSTYTFQVTATNLNSNISNNLNFTFETKIAGLFCQFNNQGIQNIRRDLNLQIQCEDFDSKYEGQSDPDLNIEVACKDLSFNTSCKNEQQQIINVNKTDSFQFIQKNTISTFTVQEWTVTAKKFQQISNFAFVIVYLDDDFPQLELQFNKGYLMRKISNYELLNFTYLIPFEKKIHLLDLTITIIYDYQIIEILQPKFISHHFKIFNSIKELNFGDKINLKFAAQYTNNIMPSINNIKLSINQPVKCSKLLINRQSDFALTDFLVATTCEQSNDSPYKYQLRQFLRESDLTDFLNGISDNSLIFYPFQTQNQFSIQAPSSIDSTKIGILVEVLDNGGSITHKFEKISIKPAKINCLSIQLKNLTLQNKITLLFEAMNQKCDELHQQIYLDLIKSQILPEKNANILKFQANKLYQQFLISQEENKTKNRLLYEEIQQKCLDLNSNHLFITQNSTQLEVNITKKIENFKKNSEDFNRIQKYFIKMKKQCEQELQQNSYIWNEQIFQQYLNSQDCLKSLIYYLDDIYSDFSLINIKDETIDQIIEELLRFFDIIAEETQNMILVNEKPLVIKGFEIIWKIKRRTKQLFNQQFNIQQTQEDYLIDFIQFEYAYLQTNPLRFNLNLEELLKSYFNDQTIQISPDNYYQIKLKNFYNRRFQEYENFSSIYDTQFGSYQVCQNTSQLLSEYEIYCVIRTITGKFYQCNLNRVQNNNRIELHCECDKFGEIFLTTSTKLIVNASESPKQIYNLELASKSEDILPLLLSTCSLSLIFLSIFIFQLYIDKKVKQERFDTEQSNLNPPIIKQKNSLIYHGTSKVYIEKFKQIHQTISLFYYKDKNIEFSYRILEVFSQFNLQLTLTILECYLLNIQVLLICVFIILNPLIILLMRIFYKIIEAIYRFKRIAAFISHLLQIILLMSPNLIIYIFYISKISMQSEQYIFAIIYLGNILISQILIEPLTVFGRILIYRLIAKSLKNMDLNPVFHLMHFFVMHNSLEEIFEEFTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJL4</accession>
    <name>A0A8S1KJL4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-related protein Rab-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Small GTP-binding protein rab1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0220012</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Protein transport. Probably involved in vesicular traffic from ER to Golgi.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJL4"/>
    <dbReference type="OMA" id="IQQKNNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01867">
        <property type="entry name" value="Rab8_Rab10_Rab13_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001018">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8054403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="211" mass="24101" checksum="EFD4ED5765F51E7B" modified="2022-10-12" version="1">MQNKESTEYDHLFKLLLIGTSGVGKSCMLMRYVDNNFTNNFYNTIGVDFKIKSIFLENKNIKLQIWDTAGQDRFRTITCSYYRGAHGIIIVYDITDRESFDSVKMWMSEIDKYAQEDVIRMLVGNKCDMEDKRAVSYEEGEALAKQLKLQFIETSAKLSTNIEQSFLTIAKNVLEKSQNSIKAESGQNMKIGQITSTQVIGNTNKKSSQCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLC2</accession>
    <name>A0A8S1KLC2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLC2"/>
    <dbReference type="OMA" id="SEIRQRW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1094" mass="130090" checksum="FF42D9A588A81FB6" modified="2022-10-12" version="1">MIRLKNIIGSAIHIQTSLFRFSTQTPQPPPPPSDQSQKKVLFSGKSADKPKKQHQKSEQKHQQQQKKESNENQQHEQSPPQKKQERHFDRQQQFHKQKVDEPRSYKQDRDRRSPDQQSQQQETPKRTGILFGNKSRDYKQIGAKYLSKRSFNQILKKKQETEKLNEKIFIDQLNEAQNIQNIHERNSKLIDVYASQSKWDKVKDIFKSEKQFNSGVYSAYINQIASNYVDLSEYEQLKAIINKVGDKVHTLQPEVFQSIYFMIFNLDLFPNEKAKELEYLIDIVTTHHLTPQFDLTYFQTLFEEQSLESEKTSHLMVIKVFNQYFERHAQQNKGKSTIDLPNLNIAFSVAVKSTIFYNTNDAQNFLNNLKSFQQLAENNVKLDDLVRYAQFISLKENSFTQLIDLFKGIRNTNFIFLFEEALKKLDTNGMEVKAIDKQQLEKLYFEKSENLPVRQAELFGLFADRFQYPELTVEIFQNHKQNKQNEYSSFLYQKAIGVLIDQSGGKHLSNSVMTLLQEANSFKVPLSKVNYQINQIKAHIKEGQYQLAYNLFTQNVIDDIILAFQREKIRRFLYKYVQKIHIGTKFRLRSNMKTYQKKIKHIDEFVKYLSDKQLFDFYKEDRSDNFLLESLIQQEDIFEFNKQITYREAFNQIKTEVAHGTFDYKKLIQPVTENEQILREVFDNYEYHVKIEKKLLFDERKRKRIYEVINQLRQRAGQRKTYEQTLAEIEGLFKKSTIENGLMRNDLIEFPEVEQIQQMQLKMKKELKQLGLPVEKYKANVITFQPLQINSQKEQEVIEQIPNEVLKKDGDNSKQKGKSQKTAFKSVDKLIDEYIAKARDDQKNLRNLDKWRRYLKTMYSGIQFKHKMQTDKNFFKKMIRQKYQFGSQDIGMKELINLKLKKKMLNRKRRWFTRNRLREKPPKISVFINGRVSLSDFQFMKYIYYNAYTQYDEYINVPPSNYQNEIWDLLAWGIQNKEPMAVKLAELYSHTCGAKMPEYLTKSIANFYKCEIGETNSEIRQRWVQSLEIVNDISTNKVYSHTNDQAFVSQQDIEFLHALECKQEYKGICRALLNTTHSYRKYVRYGVSQKELLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KP36</accession>
    <name>A0A8S1KP36_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0240346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KP36"/>
    <dbReference type="OMA" id="LIEYPKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF197">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Dienelactone hydrolase" evidence="2">
        <location>
            <begin position="163"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8056507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="318" mass="37267" checksum="F1569DEDC5CCCB45" modified="2022-10-12" version="1">MDQLAYQLCLILTLLMLFLVLMMLFSRSKRFRSFIQRIKSYLESQLDKIIFPARSCNEYQNISQQDLKVFSVVRKNVGEEIQNQRCNNIELNRIVHYAYLRNNSFPNTEYNVIYFHGNGETIDEAAYFVRNLMKSLQFNAFLIEYPKYGIYKYTQTSAKIILEDSILAYEHIKDEYQIDESKIFIFGRSIGTGPAIHIASQKKCKGLIIISAYKSLKSLIREIVFGIGYLVSKFMNERFNNEENIKQIKCPVLFIHGVDDTLIPSTHSIFLQKQLNNQNKESKLELFPHMAHNNITEFIYEISEKIALNFRELSKPND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRE0</accession>
    <name>A0A8S1KRE0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0240310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8056435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="252" mass="29589" checksum="63D1C4F15791EF8B" modified="2022-10-12" version="1">MRKQQQQDEAEEDQASLMPIKNHIKRIEEMLVLLNNDLVNDAKDIDKNRQDIRSLGEAQNKERTELHNSLLDHCHKIVTDMRELNQQSKSETYNLKQQVINQXELLQPWQQDQNQNLMALCITQQRYLIVKQSIFQDIFKLQMNGQKEDLILVLYQKKMNYNQALSFVKSKRPQINPNKGFSNQLQAFVAKVSQPPLSKNSRGNHFQQNQEYYDCNHYAPINRKGSAGILQQNMNFYKSITKSLSQSKNKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVX7</accession>
    <name>A0A8S1KVX7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0230046</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="TYNSSKC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051581">
        <property type="entry name" value="Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040673">
        <property type="entry name" value="CCDC81_HU_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028034">
        <property type="entry name" value="HU-CCDC81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524">
        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524:SF6">
        <property type="entry name" value="CALCYPHOSINE LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14908">
        <property type="entry name" value="HU-CCDC81_euk_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18289">
        <property type="entry name" value="HU-CCDC81_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="361"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="631"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="753"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="848"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="888"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="1167"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="1264"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="1299"/>
            <end position="1334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8055174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1672" mass="194706" checksum="6E6453FD06B7F242" modified="2022-10-12" version="1">MNSIDSYQSKATINAQSIISKIATRNGQVDNGSLIQALDEETVSAAWNAFGLHISRQLRMGRGVTVNKFGTFSFSAPDVVLDGVTNPVDRDKQPRQPVFLVAKEFVNGFNLKTAIAIGRQLRPYKVQTSGKIQHAIVNWAEIALYASQNKDSAKMAVERVLKSLSDIVRNRDVVEVEIPCVGTFFVKAHCAAVQFLESLMDGCKEITKRPLSERKSKGDMRLTQQYLQQLSKSTHPEENNFIIDDNAKNYLTNSLGIELPETRPKTAKHSQYSSKKGPLMSKTIEVRPSTQQNSRVGFTRSVNDKVYALERLKYYIRDHALNIEDSFLDLCQQAFGKTSERKVRMNFDDFKRAISQIQLPLNEIQIITLFQTLDVNNDGFIDKFDWLKAVVDKKSHVNYIKDVVYKFQIHTDDLLQRMNLNRDHPPINLQQLKLALMNLDESLNQHKALKVAKQILDGKETISMNDILILFNTVEEEDKLYDLSWFKDTLHKMRDHLINPQKLKTFRQSFEYFDEHQEGNLDTANFKTVLMESQLGLNVQDINRLVRYLPKNRDSLINYYDFIQMIMDVNKQMDQKDTAKDLVDFAQKISKYLTQKKFTVIQFLQQVKSGYGSCNIESTSQYLEKNIFTQLPHEECLEYCREMDVDGNGVVSDEDMNIFIKRYSYFNIRKDQSQIEQIAESFKIKPQFGDTLSAIEKTCVQSKSIDLITMGQKKMFQSLVDGMNQSKSLFPIEELPESKFDQILKDLRIKLARKGMSYEELFTFLDTDHNGFLSISEFYNIDKIMTLSQPAKDGFFAFMDKQRIGLIDLNTFLKFMSKSIIQQMPQLSEDDWDWELEILIKIRNWCQRENITIEDAFRTFDKDFDGQINKTDLRTFLKDILKIEEKEITEAKINRLFKLMDQYKRGKITLMDFRRFVEEGFFYGKNKQIFGQNTTLAAKQQQESRSSFDWKMNARQQIGLIISRHYPNVKESFDIVSGYRKKLVFQKFKKWMDEKNVLAGFDLTEKLVYEIFSDLDSHKKGYLIESDWLNAFAQYNWQDQMIKEIQDALSTYFSSIQNAIHYFQMEHSHIITKESFYKALQALFPKRFVEGDIEILWNRIQKNGSLSNQAFALIFGKSGKIYEQAEGQSQQQAIRPATQGGLSELPLDERNQVNISLLDKIRRYLRNSNKNINDLFKQYDPDNTGFITNLEFRQVIRSLNMGLTFQDVDILSAMLDTDRNSMVNWKEFSKKLDFRQADNKILERAGIHLQRVNDHIYHYLLSPKDAFRQIDAQHTGFLTFDKFKDMIEMLYRLATEDIPPFAIIKDLFEFIDKRRDGHLDLTEWMDAFSKFSNPNEKKRPMSANIRVKKNSQKSLMAQTDKNWLNKQRAPSQQQFEGLTDDGLINAANKFKTFLRKPPAKSSALQYIENGIWESSKEFDKTINAIGKNRKYLLEIFKHLTQNGQIPLTEDNIKQEMDKMLRSQGIVVRDEQKMVGLIINFCLKFTKIVQMEWIHSPEWGMMNEYLLDQLAILQYQLNKNLKKSPYQLFKTKDFPRIEKVMSSNKIRVLKKSQTYNSSKCPFVPKNTERPSYYITPPSKYKKPLTNIEELNIDSNCSTDSSQRLENLSPVYYCPKGKEPCGELQVVLNGDLIQVISQTKSRKKSRKFSDQETYAMSNFIAGPKCQEIPCPQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXV0</accession>
    <name>A0A8S1KXV0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0260058</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Fe(II)-heme o + 2 A + H2O = Fe(II)-heme a + 2 AH2</text>
            <dbReference type="Rhea" id="RHEA:63388"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:61715"/>
            <dbReference type="EC" id="1.17.99.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:63389"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Porphyrin-containing compound metabolism; heme A biosynthesis; heme A from heme O: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXV0"/>
    <dbReference type="OMA" id="AFVCYSW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120547">
        <property type="term" value="F:heme A synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023754">
        <property type="entry name" value="HemeA_Synthase_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="381"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8057466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="413" mass="47457" checksum="1A760B4ACDBCE3C3" modified="2022-10-12" version="1">MLLSRIRTFKNLTTTLKPLQNNRYYKFSNAEASKNAKFDRNAHFEDPVNEWVDPSKRWLLGGWLLLCAGGVYFMVLLGGYTRLTHSGLSMTKWKPIEYIYPRNQADWEKEFDHYKQFPEYQHTPIDLDKFKKIFIVEYLHRVSGSTLGAIYVLPLAAFTAMKWIKPQYAKRLGAIGGLGLLQGLIGWWMVKSGLDRPKPEYQQKPRVSTYRLTVHLSMALSLYSLLIWNAATLFRKPQHLQITPENYKTHLKFRGLGIGLIHLVAINLLTGAAMAGIDAGKVFNTWPTMNGQIVPANLFKASPFYKNFFENVVMVQFNHRNAAYLTYCFGSYITYLAYKSNLPKQARYICYALYGVLNYQLLTGIVALLNQVQVHSGSIHQFNALNVLTASLLLCHSVRRPSVPYRNYLKQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYG6</accession>
    <name>A0A8S1KYG6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0260158</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYG6"/>
    <dbReference type="OMA" id="IFSHSWL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000574">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="156"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="413"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8057666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="473" mass="54318" checksum="B2F7693282BB942E" modified="2022-10-12" version="1">MNNITASLKDLLPSNQKAVIKFKITPGKKIDSICQCNLKTCLICSRKRSSSMLSLKSIKNSVVVDENSPGLKRTDNKNNFVIQSSLLSKQLSRQVSNHQIQNQFYNGKINQESKGSTNSTTWIMTNSHSRKISVDSPIKEIQQQSLNPMGIGRHSFKFLYVVGKGGFGKVWRVEMKANRQEFALKEMLKTKIISKRSVNSVMNEKFLLEHLKHPFIVNMHYAFQDRENLYLVLDLLRGGDLRYHIGRMKKFSEEQTKFFTCCILLSLQYLHQHGIIHRDVKPENLVFDKEGFLRLTDLGVARLNKDSVANDTSGTPGYMAPEVMCRMEHSFPVDYYAVGVIVYELLIGKRPYNGKNRQEIREQILAKQIQIKENTPGISNKAIDFVNKLLIRKPQQRLGFNGIDEIFSHSWLYNFPWGKLLNKEIRSLYVPGSIDGNYDFQSQISADSEPQEDSSMVLRRKSVQGLFEGYKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYI7</accession>
    <name>A0A8S1KYI7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYI7"/>
    <dbReference type="OMA" id="NNFWPRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="249" mass="29632" checksum="0075BBB844EFABE4" modified="2022-10-12" version="1">MSVKVQLIHSQWDDIRKVLRATQRKLTNSNNLSLTNCAPIQDQVQKREANYSDNELEFYNNNPHALLLIKDTCRNTRRKFYLSQKRRELSSQNDQEFYSQLQSRREQVKKQRILYSNVANFEKAIKQITTPKQGMTRLSTRMRQHSQEIQPNKTNQFKLNQLDTNKINKVIPNETHYGYSSSHQTQIKKNSNNLWHRIKSPALENKDNFTQILKKRFKKQNDLFLDISCFNYESLSLLNQAKTPTLYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L113</accession>
    <name>A0A8S1L113_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0320031</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L113"/>
    <dbReference type="OMA" id="PIRISHN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF408">
        <property type="entry name" value="OS09G0560450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035811946" description="RCC1-like domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="121"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="153"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="174"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="234"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="364"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8061590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="415" mass="46095" checksum="1CC3E9ECED2FF703" modified="2022-10-12" version="1">MKLRKLGLSLLLCSAGSYSLYCNEKTKSYIWGNGVYQALPGQGLSFNNFIPKQLIDFDDKNLQKLFLSEQFESGITGNGDGYIWTAHKLSSQEDKNINDGKRQNIIKINVPKGKIKQIKYTKQFVWALTNNGDVFQYQVKDGQFINTPKKINSLQNIKQIECGEDHFVALTNDGSILTMGDDTYGQCGLGSNNRSTAPPFYEQRIRNPTKIENLPKINKIICGSNHTLCISAEGSVYGWGSNSQMQLSHSEEFSRVGEPLIAVYNPLRISALMDSNNQVLDLAAGNEFSVFVTKNKQNSETEVFACGHNIRGQLGCGFVRHISDIVKLEGLSNFKININGKQENVEVKQIECGYNHCMALTNVGAILEWGDNEYGQLGNKKRSFTDKPIVVSQFVQNKVATISCGSNSSGVIIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3N8</accession>
    <name>A0A8S1L3N8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310296</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L3N8"/>
    <dbReference type="OMA" id="WKHHNLN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="80" mass="9249" checksum="001E60BFBFC30028" modified="2022-10-12" version="1">MQMDIPNISRTSLSSKKLSQRFRIDKQGHPILRGFKLHSVTFIDDVVIGTTVHQIILVDSWKHHNLNDFRKDSSKCCEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L531</accession>
    <name>A0A8S1L531_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0260208</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="3">
        <location>
            <begin position="131"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8057764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="207" mass="23214" checksum="6C3A9F06BB58F7F7" modified="2022-10-12" version="1" fragment="single">MSQVSQRQRSPSPQSPEKVAQREKAAQAMKRRNQTQPSFFEKYAYHLVIGAFGFVCVYALFSILTRSSKKLTTTPVIDEEEIASHNSLGSYQQGPNDFFKDWKFSDAKFIFNNHLTFKGKIPQCSESGVIIPESYNFREVQPECAQPIYNQGNCSSSYSIAAVSATSDRLCKVRNGEFQDQLSPQSPISCDNKNYRCGGGSVTRVXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5K2</accession>
    <name>A0A8S1L5K2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0320299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8062041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5K2"/>
    <dbReference type="OMA" id="NQIFAQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="362" mass="43187" checksum="DF04CD379559A2DA" modified="2022-10-12" version="1">MFSKLILWFKDLRMSNQIIIINFFIIIFAVFLVLVTAHIQQAIFFTYIQEVQQALSLKQEKSIVNNISKELRLYIQQKNYQTILNLWHSQQMVYQFIQKQAQYQIKVNQFQDCIEQEEIREDNIIYNSDIICQGLFTKQYDNQAILLNKTLSLLSPFSQIFLGDEQNKISFIDVPNNQIFAQYPRMYKYKDYIPNTRPWYKNHIDQYEINPNQQYYYSPIYTSVRSFMPYFSLTYSLIINQTLFGITSQLQEIYDKKIQSIPMSIFLVNQNGDIILTTMPSISQTNLLITIINETYTGFNQTDWDFIKKNSNQKISQETAFYLENKIFNKTVFVNAFNFEKENLTLIVQDDQSIFKFQKCEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9R1</accession>
    <name>A0A8S1L9R1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0330129</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9R1"/>
    <dbReference type="OMA" id="DRKIIQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="631"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="167"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="374"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="492"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8062423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="689" mass="81484" checksum="0C675508D25A3DE8" modified="2022-10-12" version="1">MLTDSPSYKRSNKENSPKYCSIIRQSNISHKLINEVLNSGSDYLQDQLEQIDRKIIQYQCNIKQKITTLLGESTCSIDQQIYQTKLQDLRMEEQNYQQMQSDIDKQQSILYDYYETEFNKLKLQYELVTQEHQQFIKQRNEIHQTYNQTLETKAILQEDLNGKLLQRAELVGQKEELEEFVEQLKNEHPQLTEIVESIYQCDQKVKDINKNINNITNQIQSLYCQQDEKRKQLASFHQTTLLEVQVHQQTQKVKSLRNKIVPIQKSLNLQHPDSILNEFINYYGNSPIHLESIEFQTKFSTFIQQFRNNLFKQGNQGNNWGSIKEFVFQLEQFILGSLSQRLLQQQLSDLKHSLKQYGDELLLSNEVCTIDYQVEVKRQQIRELEEEKSSALYRREILYDQFQERITQQSEEAFQQYQDQNQEELNNILTQLGNAEYKTILDQTMKEIQQLMQDQEEEQFNNTYKLLQQYYLQDLAIKTNIQMVDQEILPQLKNIQQSINNAKKEFEKVNGSEKPYIERQNKIEQEIEQLEQEFKKKIDHFNNQETELQKYLNTIKLAIENTQEQLLSKNKPNKALLEQEILQLNNMLTQLQEQRKQLEISQFSKSPQKTDVSRRSSNGGILAISKSLNQFSKQRKEPTPNSFNNSVVKGITPSKESSNLYHIAKYPKTNCKSSQELRLKSNISLKSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LA38</accession>
    <name>A0A8S1LA38_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0360256</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="QPIVHHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053080">
        <property type="entry name" value="PP1_regulatory_subunit_27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46899">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46899:SF3">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="1016"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="801"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="867"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="807"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8064698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1324" mass="155436" checksum="9F4AAA4E9AA99FF8" modified="2022-10-12" version="1">MNQNYIDQVFFTKQFYRAFGNYFGPHTSTPSIASEYLSSTRTQTANLNKQNSLAKPITSPQSVRKPIHQNFERVKTQTQHISKQQPIVHHIKSLSSDLKNQPQQKRFIQQKIISYSESEQHPFLIIQKKARELRKIESRKSILPSIYNDSQHSARLLETPPLLQIPIIQITEPLEQHSSKGQEYESDKDDDSLDEEDFPQGQQRMKKLSVRLKFKNAVQSQVSTLFINKQPKSLFNVIISPEFTLKHEEERQKISDAFEAGNFILYKAKQFQHKEVTNKKKLFVSLLNMKHPHKINNIVNSQIAKLNTTNKAFRRKIKMILTTTENNKEIEQPQKFSFLSKYKKSQFGQQGTNEHIAQGINLNKDQFIITITNDKLENEIQIFQRPKFLIQIDQVNEELQLALSPPKLAKSPSRRVLTHSGSLHQSQKHINLRHSISQQIIKVETSNIQIVPNIEENHNLDFNQEFSKSNLYVRLYYQSKLHKLPRKTTFTGMNLQEIEDYFTSQQQLIKLTNINFQVTEVPGFEQLGQVLQQNLEIPKYIIYKPKLCLDINLDQTQTFSSSESDQSLSLDLLLEEPIQSLILNKFRPGSQQQQESGNQMVNKKQSLNNLDKEKDQTIFIEDDFKNLKSLSTGQLTLIKKARNNFLQPLEFRCILSTLDQNTKNQLLTFPQKAKPINEMLNSLYSDNDSGAFNQLEQYNNQEILKLRIVFQLEVRYEGILVGRYQDYKKIIDIEHQDLIETLQNLPEIPKLKEIEYQSKSQKSQMQQQQQYQKQQQKQQPQQIVKTDLQIRQIAQIKKNLHNKTQLKKNQTQTINVQPNLRTKQTSNSPDQNNSKLTMQYSNQLTNQQTQNNNNNNIITQSTTQYLTKPTNQSQQSVNISGSKTIQKSKSNADLSETSSIKSSNSRLEISSQQIIDVDNQKHQPKIQQFQELPIVKKLNHHHTKSENLINKIFEQKIQSMQQHALRMRSHAEEQTKRQKYDQKYQVKFAIEDNNFQEFMENFISLPEMYIDYRFQNNDTFLTLATQSGNKEIVKELIRRGADINIQNVFFYQKRMMEIPPCIWQQHILIILQLTCQCFQEPLLTFQISREGMRGIEINIKNNIVNLMNKGAEKKRTSSFNKQTINQIYDRYDTQQKQFYCPKQKKDVKNLINNLKLKSSYESTLVMISQNDSLKNVLNGLTNKSPKRLMKKYIKKTPSTSLHTPADFTIRNTEQQSELAKQQQSILNQQSQQISYRSLLQHNKRNQWVDLINQKSSNYIQQDQIAITEYQAQQDIQMTNNCDWKQPYTYYKEQRHHSEMQIIENLEFDSWKELMMTRIVLRQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBQ5</accession>
    <name>A0A8S1LBQ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0360137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LBQ5"/>
    <dbReference type="OMA" id="CCIDFTM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="262" mass="29726" checksum="612F7EB4732833B8" modified="2022-10-12" version="1">MSLTKREISKNHMSSEVNILNQVEQELIRKRPGSGQPISTKKSPLPLRKPGNSGNQRMIIKEPEQHMKPRQLSNNSLKTCSTQQLPIIKSPSQQQKPPIKIDKKIIMMKQEQVITSARGTLTGERSQRNSSNQNLSTADTHTKQFNWIDDQLSSQRESSLNKKRLGSKEKQTNNIQNQKSKLQDNHKNVHPPLNQPKCCIDFTMTAGKNDLSKMLDSIKMEIKQISEQQHQNGVILNMSEIKEMADDGCSSMGQSTVNQFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFJ5</accession>
    <name>A0A8S1LFJ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0380005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFJ5"/>
    <dbReference type="OMA" id="STSQNEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="104"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8065649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="328" mass="37997" checksum="2DE4C3728CAA543E" modified="2022-10-12" version="1">MLIVKGGPPQKIGKTQSVHHMPQDSHLIKLNPILTLKTEGCFDSTLNQIYNSVSDTEYVYGRIFKQLILFVDTKFSELTEKIDNFDNLSDINTHLLRIQSSQVSPIMKQRNDDSPSGIVRNKSSGSLLQQDNTSLSEKGKNSPSKKILQRNGLSSSNAMDINRLNQFEKKIRQTEEKQTKFEQDQQDRFSGFQNRIEKIIRSIQDKQQSLQFSDLYRGIQEFSENQKQIVSFISTSQNEMIEINKKLLSTSREHQDQINLIKNTVEQTNLAYLACNEKIETLQNSIQQIDQDLLIILKCYKDVLNDVFRIEILEQQLKRILNILNNYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJS6</accession>
    <name>A0A8S1LJS6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0390070</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJS6"/>
    <dbReference type="OMA" id="YSGEIYW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000104">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000224">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="6">
        <location>
            <begin position="167"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="200"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="361"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8066402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="494" mass="55603" checksum="1FFDBE13330E0378" modified="2022-10-12" version="1">MLLIAALVFLGTAKHFTVDLKSRHTQEHLIDIVNMLQHSHAQLLEENHYEKIGKGIEGALQFETVMLEKNIQKKNLKHQNHHNKKQEHHHKKRKNTNMVVNESLMDVIPLTSEEKGIFEESGALSPIDIHIEQQMEHQSQKLLTTNRNQDGLYIADVPLTNIANTMFIGELQVGSAQGKNAFDVIFDTGSALTCIASEQCKDIGCQKSKRYNRAESQSFNEIGKSVEIVFGSGTLKGLINRERIRVDGLDLKDALFIEVTQQIGDAFHEGEFDGIVGLGYPHMTGVPTLFDYMIQQHKLHSNVFTFHLNRATGNSGSQLIFGGSDDSQIKGEWVYHEVHEQFYWSIMAEQIKVGNKDTGICTHQHKCKMVVDTGTTLLTGPTKDVRTLLSMIRVEPKCQNYQTMPDITFVIDGHDYILSPKDYILTITQSGVEAPYHHSSMDQIVGCAGTIFPLDLPPKQGPLWILGDVFITKYSAKFDRDKNRVGLALNKNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LK17</accession>
    <name>A0A8S1LK17_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0390131</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LK17"/>
    <dbReference type="OMA" id="CLVVMNR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070736">
        <property type="term" value="F:protein-glycine ligase activity, initiating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051437">
        <property type="entry name" value="TTLL_monoglycylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45870">
        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45870:SF2">
        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="338"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8066522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="735" mass="87316" checksum="09B00DC867733202" modified="2022-10-12" version="1">MLDNRASYFNVQGNKASINMNIKMFQSMKTKGHLIKSKSKEPIRGQLKTLKRLEDTIEEPQMQQRALTTQKKLAPLIEEPQQKNEDLNPLRYNLIQLLVLMGKKEYYFDKIVKDDLKIIRVSTQSKENPEEHLQKIKKFLSNKVRYKYRSTFKMLLQIQLELLKYTPKKKSVAIKNSQREFEQDEEDDTPKKKKISARGIQDYPTWKKKNNVAPNQKVFIITSGYHALRDSLLERNWAENEDAYSPFFDLKWTCSLRSDDFMNLMEFQQINHFDCNQCLCSKYGLARNIRCINNWESFFPRCYDLGDLVDFSDFIEDFKISKIHSILLDFEQSLSNQTQNQTIAEPNEDEDDQNNTLRPELYQNNNTKLDDQNNTIRNKEEKQQPININQLTFRQRFRIRLCLVVMNRVNKSLSNMVKSILRDEFPFIHPGEWIVLLKDPEKPREILIQDYIDQQLKLNGYEDYEGVQFLQKYVPQIKELLKQLSKNPQNSLQGTRNIWIVKPEYSSRGRGIRCLDDLYSILDNVNKETMNYVAMKYIENPLIIKNRKFDIRQWILVTELVPLKIYFYNECYVRFSAEEFDIDQFHNRFAHLTNNAIAKYSQKFHKSEIKGNMWTQDDFQQYLIEEFGWDVFAERIQPKFKEIVINSLRCCSDQLKNRKRSFEVYGYDFMIDDQFNSWLIEVNMSPSSDTTTPVTAQIIPKMLEDIVKVVVDNQNKTKKKIGGFQLIYNSDTKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKC9</accession>
    <name>A0A8S1LKC9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0420009</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="1670"/>
            <end position="1855"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2933"/>
            <end position="2965"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2976"/>
            <end position="3018"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="3020"/>
            <end position="3061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2063"/>
            <end position="2109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2182"/>
            <end position="2238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2270"/>
            <end position="2293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="719"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1104"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1513"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2093"/>
            <end position="2109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2185"/>
            <end position="2201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="2205"/>
            <end position="2228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2271"/>
            <end position="2290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8068147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3327" mass="392768" checksum="C8061EA695CB13C9" modified="2022-10-12" version="1">MLMQRYHEQSLRGGGSAFSSSNNFQNIPLNVQIEAQLHSTLLNRWLVNLSKRLEEIEHVKEIMSTEEGKSVLCRSIAWILDLEQLFCYFQFQIDLVKRHKQIIEKTFQYFLELASTIIQWDRLIALKILKICNSFSRLIFIYYSHSNERSQQQQKDKWSQLTSSIINLINLDCEASLFYNCLIYELTLIQACIGYIPTEDDPDERNTSTFDILKEIFNSAVSLIPSPDLVSSISKGALFLYQQYQKKKSMEQFQNCLFLEQLKWNILMSIKAGVIFNEIEERISNNFNKIIKNGSDWAIQYSWIKMASELMACKPQISKIKFMSLQQDSTQIITWEQAQSDNLIHIIHKDFEIAEIIAQGQTSNIGIKLQSISRQYQNLQNFFMNGNQIIPKFISYYTLESGKDVLKFDNESYHDYFQMIQSLRQIPLEKIAFNFNEFLIKFQSFLISYYQIEPKNINLKDFEYQLILAQENYQSLLNDINKLQISRDCLNNIIQIISPLLIKSDELIKQQASIYQNCFQTFLYNYSKIQSKKFFDNKRDIYDQLIIWLQQINLCQEQQIHYQKQFGKKMPDDYFNLSKIQEKPFQIQLNYLLFAIQFCQQWNHQIQLLTSLFTKEHIMIKQQIIIAKSTQEAEIKKGNLIEEFISRQNTNFEYLHSTYSILQMLYEYFYELKTLLFDKLSLEEFDKIVKKQKKELVLNLKFDFNLYFQYQINQHQQFIQKLDTENEILSNQIKQYQNNKNGQMLNYFCQVFNISLITINQILNFDFDSVKKMEMLIDLVNKKDNQVYQNVRSNSLVLQTQIDLLVKSKEKVEQSNNVEYVEKXIKFQSFLISYYQIEPKNINLKDFEYQLILAQENYQSLLNDINKLQISRDCLNNIIQIISPLLIKSDELIKQQASIYQNCFQTFLYNYSKIQSKKFFDNKRDIYDQLIIWLQQINLCQEQQIHYQKQFGKKMPDDYFNLSKIQEKPFQIQLNYLLFAIQFCQQWNHQIQLLTSLFTKEHIMIKQQIIIAKSTQEAEIKKGNLIEEFISRQNTNFEYLHSTYSILQMLYEYFYELKTLLFDKLSLEEFDKIVKKQKKELVLNLKFDFNLYFQYQINQHQQFIQKLDTENEILSNQIKQYQNNKNGQMLNYFCQVFNISLITINQILNFDFDSVKKMEMLIDLVNKKDNQVYQNVRSNSLVLQTQIDLLVKSKEKVEQSNNVEYVEKVKDFENSVKTIQQELLFINNSDCTSLVNELNKFNEFLVHARTRQKHQLDSTINALKKCTLDFIDKIVNLYKNVKQSLKKEIITEMCESIDERKPIKANLQELTNNFKDQIMQAQQSIEFSEIDTLQNLVDLMKFSDQNAIICHQCEDQISKLLQKFQLLIKNLKSLNYESFCKSYADKLYFICQNQKELDRFLKDQLNFIFSPFQNQVPNNLKQCKQSIAASILNYIEGNQWRVKEGFVFQCIQLENQLHEQNKQSIQKVLILIAVKETNLKILVTLKNKQIVQQMYQGFSQNWPELQSQIQQDLVNQINKLEELQYNISGAETDRKRELLLQEHKQLEEQLEQQILNVNQIGDALGITITFLREIRQDLKKIQYKLDKLLNKIDEIVQDLKQLIGKTPKQLLEIRMQSILQQKVINDFNNVYVILRNKELKENFKDEDDETTLFNDIYQTQGEIDEFLQQPKDSLLIHGPAGSGKSAAAKKIEEYLWLKYQQLNLDEWSKDLEQIPVVPIFIQLASLKDPYEKAVEETLSSNIYNFDWKQIQAFQAQVEEGKVAVIFILDSFDELSNNQINLIQHNKLKNWKQQLLNLSELTQSQQRIYNLNYPKIITTTRSEAFELNSTNYRQWFSSEAVLDFTKYKELRILSFDDKQKQEYLKQYSYTQIKKTLFDFFEKYSLFQKRGRNLISEVEKIWNIIEEKLERFNHQEQSNNICLSDKEINIIISVLQTEFQLQNKQDQFSNLERQLMHIQTPQFYNNNIIDFNLEQLLKTPFMMKIFIDVLPQINCQKQEIHQEYFITSYKLQMYEKYVKKQDIKAIRNFLEGISQKQWNEDQQDEQNNQQHIPQQPEGLEQIGEPKYQPEIPEDPPKLSSEDSPGYPHQDSFQIPETPVTPETPETPVTPETPYILGIQKMLEIPEQPEINEFSQSDKEINEPSQLQMERRLQQIKKLSTKQKMNQSQPVQKKLFFQKLFGHEDSNKQNQNQNEQAKSSKSQNDNTRQSDEKNYYREPDDKCQNNHEQMRQSRPINIRSQYREEKYYQKSKQSTNYEQFQEFIPQTIHPPASQAIQQQSYDTSAPSKKSYNDQPHIPYLNQTQINLKLEEERLNLQTKIKEHLTEQGKKIFQNISQISQQDQDQLGDDKQILLRCYKSFKLTQYDFYQYFLENYIQNQIKKLLTIQHQNFNHNFISEVIEYARLLAQYLMKHQLTTIDTQKVCNQQRVFKDKKIIQHSDDPIYQELINYNSEEIAIIIKCLPLKLTGTILSFQHKSIQEFIYAKFIITNLLAISSIINQYKQLQAKISLQNFKINMNSQKLKKNQFILICIQIYLFNNLDQTSNKDRARDIFIDQFNRTFWHQYQESQNIYQSTYDLLSSIDISPLNSVIMANQFYMGTIRFIKEYIENKQDLKSVLHFLVLISQISSKFINISSNSIQLLTYMQELFYEKSFFGIQIKDVKLLGFKCIGCDFTKSKLENVTLVGTNLNYCKIAEVEWKNIISQDLPTLSFNIGPIIKASFSFDGQYIASLGLQQNLVIYQISTGLILKNIQLKKAVQDFCWSNTKSTLVYFYEETLNILSFYEDQKQNDVNMVQSNEIEVNQQHIKFKFKINDTVVLIKFSNSDSYLLVGCQKGKIIIYKFLNQQFTLFSEIQRKSRITFDLYSDENSLIACDQNQIFLVKIQEGHSKTIRTLTQDYQVTRIVFSSDGKLFAYATTDGVIIVYSLITQKDKAKLIGHKNAVRCVCFSSEGNILVSGGDDKSIRIWDYMKGIQIGENLHGHFDVINSIEFSKPDGTIILSAGNDGLIKQWNHSDNYHVSEYLPGHEGSILFMVLSQDSHRIITKGKDKKIIQWNIETASVVNQMISLQSCQECSINKLCSSCQLSPIIIVNDDQHIITGGFGHSISIFDIYTGNQTNFTISYLKPKGEVIILTQSEKYHLICSGTMNGEIKIWDSFNGRQFSNKITLNYQILTFFFDLEQNLIVLNQCGKYTKFQLDLSGTIAQACQCELICEASEMKQINQIIAEEIAEIQIDLQKQNFQEKFNKRVTCLALTFDYSYLAIATKDSNVYILETADLSEIFQKYSYQGVITSMYFNKNGLVLILSFEDLSIKGEQKRTIIIYLNYEELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKQ3</accession>
    <name>A0A8S1LKQ3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0420076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LKQ3"/>
    <dbReference type="OMA" id="CGNQPSN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="125"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8068277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="211" mass="24764" checksum="DE9850C4FCA07130" modified="2022-10-12" version="1">MFVRAQVPKSRTSLTPQNFKPICIEQRGDCTPTRSNNISYYEHSILIQEPSQQSLQNYTGRVEEKLQKRVINSSASNLRSKYVSDKENLVRVPGQSFCGNQPSNKELQQVINNLKKQKEDICFTLSETLKKNTQIQEQIETVNKEMEQQSLNYQQSLDEFMNEFNDLTQKISQLEEQNNKLFEQNEHQKWYLQQIQCQSSRFNQTHNFGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LNE7</accession>
    <name>A0A8S1LNE7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Chromate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0410208</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chromate ion transporter (CHR) (TC 2.A.51) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LNE7"/>
    <dbReference type="OMA" id="VWGMART"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015109">
        <property type="term" value="F:chromate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014047">
        <property type="entry name" value="Chr_Tranpt_l_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003370">
        <property type="entry name" value="Chromate_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00937">
        <property type="entry name" value="2A51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33567">
        <property type="entry name" value="CHROMATE ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33567:SF3">
        <property type="entry name" value="CHROMATE ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02417">
        <property type="entry name" value="Chromate_transp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004810">
        <property type="entry name" value="ChrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8067872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="43945" checksum="4AF1D8EF7C24F0C2" modified="2022-10-12" version="1">MIPMDTSSSKQMSLKQLGDVMFPLGFITYGGPLAHINVLRERFKNYTTEDEFQELFALCQTLPGPTSTQMVIAVGANLTHSCLGGLVAFLYFSMPSAFVMMILGLTVPNLQIPWIQLLINGFKYASIGVIIEAAYKLSNGAIKNKFHLFLWIVSAIVTTFFPTPSIAVLMIVIGAISNYVSDRQIGQQLLIPQEEQVQHLFDSWLLGYRSLVGYGISFVLFFFLSYIDWMPIAVCSNLFKTGSLVIGGGHVVLPMIQFSLSNYLTTEQFWNAFALVSCMPGPMFNIAIYLGALIGGIPMAILAEIFMFLPGFFTIFGILPYWKKYRGLRTIRAVLQGIAAVAVGFILSAIVHLIINSCSQAFVVPIGIGILSCLCLYKGIPIPFVIIGGGFINLLTEYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPR7</accession>
    <name>A0A8S1LPR7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0400238</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPR7"/>
    <dbReference type="OMA" id="THREGNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124:SF14">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_25, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="785"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="643"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="644"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8067353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="849" mass="99099" checksum="FAEAA56E9C7617C2" modified="2022-10-12" version="1">MKQHANNYFVKKIAKPISQVSTNSAVTRSNEQKPISYLTYTNSKHKRKNETAIKLPELQPSSIRAKTEQDQTAQNELKRSIVIISRKPSATLASQRIQTSPSIQIWEPDQISQQPVEEVQEITNKFYDVLCEELLLNKKQSKIKEKRKIFCKTILNEYDKFQEKSSRMVRKIIQLLKTHREGNEEKEMKSRTIIETKMAKSSLFFHAQTLYLPEQHQFNQKELLFQQQLSAIPKRHTKYSSQLTLTQQAESLKPIKSLIEEIKEKQQDPIKQASICLVSPKHPSNNKNKIKLIIECPEEQKSNKFEFFIPDYMEQDQIVSVQLLNDQIESKDTKKSNIYSMVSKNMFSKKMNQIQNVSETTMEEFNSDKATQFTRYYLQNYALKCINQKKIKDKKDSESQLNIMKQDSKQEQQIFLSKQLLDCSITKDLQATQFLEINSFIYRCIEPYDSQDSSMISDDSLIDDKSFIDSSIDQGIKLKFLISSKLINPNIIKDKFIKFMISTISDESKNILLHQEIMAFANQIEEVIHIDYDLPQQPHLEALNRLFLRINKYDRKQFLQSFFKKFENICIGLFEDCYKHQLNKKEKIFVKLCFQQQGNSLTDNTSIKYSNMLKSRHHRETTTETVPKQQTNLMILSLPPVTIKSRSQSPSNFSEPRTQKNGMQNQQVLLKNIQRKESQRFLQIQTSSKFQLQQQASKQQVLVVDGKEQSKIQKVKLTSFQNNLKQLSMDPQSLLYRNKIIENTKNVVKSLQERIFLMVEEHKLLDLKEILKLETSIDVNYQNDEGNTMLISASKCGAVLIVEFLLKNGADASIQNYNGLTAMDMALKNYQFQTADIIYKYLYPDNKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSZ9</accession>
    <name>A0A8S1LSZ9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SUMO-activating enzyme subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0460078</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSZ9"/>
    <dbReference type="OMA" id="TPSEHIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000002">
        <property type="entry name" value="SUMO-activating enzyme subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030661">
        <property type="entry name" value="Uba2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF5">
        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039133">
        <property type="entry name" value="SUMO_E1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="5">
        <location>
            <begin position="9"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR039133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8070667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="583" mass="66794" checksum="D4712982AB5ECCC0" modified="2022-10-12" version="1">MNKTLNLLYSPETYEKIRNSRILIIGVGGIGCELLKVLTNSGYKTMTILDLDTIEATNLNRQFYFRKEHVGMSKALVGKESVMKKHPELDITAIHGSIFDEKYDVEFYTQFDFVLCALDNAQAREHLGRMCLKSNRILVDAGTGGFSGQANVIKRFLHQCNNCQPSKGAPQYAVCTIRASPSQPIHCVTYAMSLYNLLFGPLDESNMLAGLLDLAQIQSCDKNDQELLNGLAIRTFNKLFYDEIKQGLKGYAKDEEANAINQNKYPISYEEGLQDSNVYTTIDQSKMDEEDDKIQPFKYYVDQFVKTFVKILQTPGVGSFKFDKDDWDSVRFVAATTNIRTFNFTKKFKNIDPRLVEGDKMKYLSVNEVRKMGGRIIPAIASTNAIAAAIQVSESKKVLEEDWDKLRMNWIQPTFDKIAPTFLPEPYNTCNHCSPKNVYLHIYCNFDTTIFGTVVELAKSFGLNDYDISGQSGTVYGLLNKQIKRDGTNERNYNKLLSKFYQRESPYANIIFSIFEQTSGRKFYWQIGCKYEFILMHRGDIKIPQISKITKLESAFQQLKNVKLQKFNDINEARMRYLDIYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LWJ2</accession>
    <name>A0A8S1LWJ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0450171</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LWJ2"/>
    <dbReference type="OMA" id="VDCCTIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046621">
        <property type="term" value="P:negative regulation of organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033276">
        <property type="entry name" value="BB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46400:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46400">
        <property type="entry name" value="RING/U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="176"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="31"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8070325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="225" mass="26659" checksum="4A9D648FDEFCF979" modified="2022-10-12" version="1">MDCPIKVSQLKSVTEQKEVFDKLFKMPTQHVQRSQQKYQSKSDLINELRQENYELKNTITELKDRIQDLEQQLKGKQNLQIEEENQEIISVEKPLQEMNEEEKSLHFALMLQEQEEMEFQNRLIQMQNNVDLDEMSYEQLQELQDKMGFVSRGLMEHQIQILLKQCTIKNKTVDCCTICLEESGNPVEIQLECGHVFHKECISEWLSREKHCPVCKRDVDLGKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M358</accession>
    <name>A0A8S1M358_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Origin recognition complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0520236</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">ORC is composed of six subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ORC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M358"/>
    <dbReference type="OMA" id="KPAKIDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003008">
        <property type="entry name" value="Origin recognition complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF23">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="249"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8074357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="615" mass="72028" checksum="99C92CC269D06C79" modified="2022-10-12" version="1">MPSRRRAKKKNKRTNKQQRSTKVEKQKPAKIDYQPKHLTRAQSKTKEKKEHKIKQESIKNENDQYNSNEQYPQSQKEKGIPASLTLTNLQECSDQISQISQLFTLQKSEVPNNISRQLSKPKIMSIRAAKLTFPEDSKEIQMDIEQEECQFINQAMIQTRCNLLGMPKQNKYKKEHSLTTENNFPSEHYEQSTHIQIPHPDNIQQKLTLMEECFTSLLECTVPDEILCRDQEKILITRFIEDGIKNNGQKQALYISGVPGIGKTATVLEVKNKLLSKKLNFEFIYFNAMNVGAPEDIYPFLYEKFTNKKETSRIKSCILLTELFNNQLETIQQNKVVLLDECDNLYTADQQVLYNLVDWPQQPQAHLIIIMIANTMDFPERLKPKLQSRLGNHRIVFRPYNSTQIESILQQRMKTKKIKQLFASNTLNYLGKKIATISTDIRKTLSVCRTAIVLAREQLLKKSVFSQIDVDHIKLAYDMIYNKPQHNSLQHFNDELKLLLIMIALEIHIKGYNYAYFHQVIQRVNQQKQLHKQDTLSYFQMKQVLIKLSQLNLIEIKEEQVLLTKSTWQQKIQNSLDKDRLFNLEDMLIQLKINIDDIKNGLSNDDLFKEFSNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M564</accession>
    <name>A0A8S1M564_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T0500188</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M564"/>
    <dbReference type="OMA" id="YKAPAAM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="17">
        <location>
            <begin position="58"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="89"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="287"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="441"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="58"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="482"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8073063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="519" mass="59448" checksum="9EB31D3FD61C0183" modified="2022-10-12" version="1">MLLNRNPQTRPLVNSTPIKKVFIDPTQRIYEDIVVGEYLEEHQIVVESNEIEVPQPFIEWKDCQFPNQLNKRISLKAYQKPTPIQASVFPIIMSGHDLIGIAQTGSGKTIAYLLPGLVHIECQRKKGGPMMLILVPTRELAMQIQEHISYFSEAYNMNSACIYGGADKRPQELALTRDPDIVVATPGRLIDFLDAQVTNLHNVSYLVLDEADRMLDMGFEQQVRKIDSYIREDRQTVFFSATWPKTVQNLACDLCHNEPINLYIGSQEVTINKNITQETICLYQNEKQEELLYILEELSKKDKVLIFVETKKDCEDLSSYLTEHGFFCMSLHGDKTQQQRDYVMKEFKSSRCKLLCATDVASRGLDVRDISLVINYDFPNQIDNYVHRIGRTGRAGDKGRSITMITLDTMDPRIAKQLVDLLKDSEQVVNEDLYDFAYSKPYQKGKRPNQRNKVNDKPIVQSKNINNTNNNTQKKQQMQFNLKPSSPVKQSTSSMPPPPELKKAFSDAPVFGFFNSKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M664</accession>
    <name>A0A8S1M664_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0540113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M664"/>
    <dbReference type="OMA" id="THCHSKN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="122"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="355" mass="41870" checksum="8553A9B4E92B58A1" modified="2022-10-12" version="1">MEKKIIEFIQECQTNIGNCQDLSQMEEIVHQLISIVYFLIEQGQQTFEEEKLKYQALNQEFFNLQTLYNQSLQEIETLKVHHKQDVEEFSLIEDRMIKQEYFIEKEKDEQINYMKSSTYEMMKSLKNQQLQLENVKKQNEDQQMEIKELRKLLQQEKQSNIGSALDHEESKLQTYLNIKGHQNSSSSCDSVKLKQLESCSSIELNPQSNQKENIQAENCSQTILRTNSIREAGHHLKYTHCHSKNQSINWVKLATIAQTQQSLSSNSDDEEAVCQKSQRRLSKDLSTECEPILFGSRNPYKDFYTLISQSVKLNLNQTKYYVVNVDMLYDQFLAEGVPFHKWYKKIKDYIQKQVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M863</accession>
    <name>A0A8S1M863_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0560125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M863"/>
    <dbReference type="OMA" id="IQHNKSH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="560" mass="65359" checksum="08FBCF812CB97B91" modified="2022-10-12" version="1">MSQKAYQSIMDANISATQLLQEEKYNQGYQILRRIETTLKQEKEAPTIPLSQINHLLSVVTNNIGCFYYMVDKFQAAEKYLKQSIEYLQLSIPDETLLITLINLVILFIKNEKYQEASRYIEQSFTELNQLQDQQLNPKIRLFLGDHSSIMTLQSLEAILLFYSGLTFYELGQSNNALQAWKKVLDILQSNTKSPYYILTKSKIQNLEVENVAKNNKGNLYSSRSHLKSDRINKILEQYSKDNEKQNQQSFRLLSQKIKKKIDNDISEYQQPKWQNKIEHNEKKIEINNYFTDQKKIQVQINVKDLLHQKSITQRDKNRDIPLSSHTSNVNQQINKALKKRPTQHIEIIEKLNKTHNEIYKSYLAQRSNNGSKTQRGDSNIDIKIKVNDKSSHSQNTIQNNKSHDKKSVDLRESPELCGSPQQFFNMLSSTQSVIKSQLMRSGSQGSVSQTSPFRLAQSQGDQPKNTSKLPFDYAKTHRPMVDTYDKAIKELYQNQYVQIENKKFNRIYDLAQSVKNRISNINRMLIQQEKNQQVLQKKFQQEFNQSQDEDSCETIIIMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M864</accession>
    <name>A0A8S1M864_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0560035</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M864"/>
    <dbReference type="OMA" id="HEWNENK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894:SF5">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE RSMI, CONSERVED SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="955"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="588"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="239"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="508"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="588"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8076297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1145" mass="134572" checksum="61D6C0D778137320" modified="2022-10-12" version="1">MLNKNQLPLLPNSTQVPITTNNLSNKNNSQISVLDGNSMKQSGTSANLTLNLTKTRITRYQDTSYLQQKTVIPTSLSNLVDDPKLHEYTQQRLKTNFYGTMYRNSQSMTSYKTSLTELDSKDGTLDLNPLLKNVKSSQLQIYLKKIIQQQSDKIQQSSTLPNRQMAIDLQLWFDYMIKKVKQSVMLENCNTQSIAEEISLIYSSCLKEIIRQVSLDCKERGQLLEKVWNSYIVLIDDLIQNINKTNQENLENLKNIQQQQLLNQTQKSYQQQYITLQQEFQQFQIEKEVLEKKNTFLEDKIKKQKEEITLLYQNIEQITEQQKKYQHNHTHSNDIITQLPAGFPNVQLKHLQSIQEGKSIVSHIMTKKAQIPIQLKNKSLSPIQIVKTSDDEQSKTPLSQLQLEFDNMDDSQLIKIKKYHRQSNIVQNVILDEKQIQTDKMIYSNIPILNEFIHNFINHGIQAIFDEVKEDEYRESISIVRYATSISIAQEDFVIETLNKSKIKQKVYEELQQYLTKLNDKYQEFQSKSIEQMLKIKEHQVVAQNLEQNLKEATQDFVEILKEKQQTIKSLKHGQTLLLKKINSISQDQLSSKRSPQFTSPNRKTPNFKHTFDFNKEAISKEFDVIKVEEDNDNSSIKSDEKDKINQTFQINNEVLHLNVLEDQDEYDDGQIQIEEKQDLNLDISINDENTLNDQKSEITQSQQLDLQLDSKKVNENRLSNVKDLVQKFGLQRQSTKKFTKPLQMRMSLLSELKQDKKKVKRVYSQNTDAANLLMKELIAKTPTKKSVRIPIIPIIQLLKIFYTLILDASKNPEGYKIPLHISIYDYFLKKYGFKNVAEKKIKQIFQFICSKMETQPKMQFMARLCYMHGEMDEAIYKQIVETVKYFNNKEVNFHKNDLIIQYDLMTEYCNEFLANFLGQNTVNQYLNSLKNKKEILFESEMITILNLYFSKKKQCEDSLQLIFQAADLDGNQLIEFSEFKTLYKAIHPDLYNKTKALHQFITFADFIDENTKDKMITLDRFAEMAIELNLFNKEKINQYSQGSQSLIHEWNENKNLIKYRYLVSNNYHKVKYTFNLLKEQIQLHIQKQSCSHSIQILWVSYKLLFERSQRVIQNYELKQLMDEFLPPEFIQITQIHNAIAEINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M874</accession>
    <name>A0A8S1M874_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glycine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0470174</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M874"/>
    <dbReference type="OMA" id="MEMQYFV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070150">
        <property type="term" value="P:mitochondrial glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.230:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000004">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="147"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8071424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="626" mass="71099" checksum="D4461FA440329373" modified="2022-10-12" version="1">MLQQLNKEVLLKRKEALEQVIKRRFIYQPAFSLYGGVAGLYDYGPVGCAIKTNIEQYWREHFIIEEDLFEIAATILTPEPVLKASGHVDRFTDLLVCDSKTGTGYRADKIVTETLENRIAKEGDKLAADVKAKYLLVIKDVDTFKEDKMKEIIAELQIKAPETGNDLTDPTPFNLMLPTIVGPSTKLPAYLRPETAQGMFLNFARLLEQNGGRVPFGAAQIGLGFRNEIAPRGGLLRCREFQMAEIEYFVDPNEKSTFKKFNKYINLEIPLLSRQLQADAKLHQPYKMGDAVKEGIINNETLAYFICRTYLYLVEIGINPINIRFRQHQADEMAHYSSDCWDAEIEMSSGWVECVGLADRSAYDLNAHSTATGQKLQAARKFKVPQPRQVIQIILDKQKIGKELKKDGMTLIKYVEALPDDEKQELSEYFQTHEEKVFNIDGKDLTLNRQLVKFEQKTMNVMEEKFIPHVIEPAFGIGRILQAIIEHSFNQRDDPQKTYFKFSPRVAPVKCSILSVVQNEEFDIVIQELTSSLKKLGISCKTDNAGVALGKKYARTDEIGIPFAITVDKETLTAQSITLREIETTKQVRVPIVEVPRLILELSNGLILWTDVLNKYPLFAAKEEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8H0</accession>
    <name>A0A8S1M8H0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0470224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8H0"/>
    <dbReference type="OMA" id="KYSCINY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="157" mass="18170" checksum="0B74AACD6688DDFD" modified="2022-10-12" version="1">MNKKNLGWDIEEQQYLDSLIPGNLPKIEKRHAENKCHKIKSILDSKIIKCSSSIQLNQISKSTQISTQTTQTAFQNTLTSSFQSPMNLTSYQALRYPPKQDVVYKSSKKSQSPLKRRKYRCSIINLSQPSILSPQANNKLYNINKYLQQKYSCINYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBH2</accession>
    <name>A0A8S1MBH2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0580259</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBH2"/>
    <dbReference type="OMA" id="QLMRHHF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF197">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="38703" checksum="ED41F8CD4B5AD85A" modified="2022-10-12" version="1">MIFPIPKNTYREGDLKELIKIPFYEDIFKFIPTSPKTKIQFHKIDPFKPPDEIPNDISALSIYDDQVDELPSQRIPHIIQTTNLKPKTQIRELRLSISPRSSYNNEFPHTLTNRSCKYTKEIPCLYLKSYTMTKRTIIYFHANCEDLKSSYNLIDFLRHNMRMNILAVEYPGYGIYQGEPNEEVILKDAEYIYKYVAFHSGIEEQNIILMGRSIGTGVACHVASLFKPATLVLISPFLSLQEIVSEKYPFLRKILKERFTNKDKILKVKSPLYILHGLKDSVVSVDQAKKLYELCRCPCLIRTPPEMTHTRFQFEQDLALPLLGFMRQLNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MET2</accession>
    <name>A0A8S1MET2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine/threonine-protein phosphatase 4 regulatory subunit 3-like central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0510261</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MET2"/>
    <dbReference type="OMA" id="AIQFSFN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030289">
        <property type="term" value="C:protein phosphatase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006887">
        <property type="entry name" value="P4R3-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051137">
        <property type="entry name" value="PP4R3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318">
        <property type="entry name" value="ATP SYNTHASE GAMMA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04802">
        <property type="entry name" value="PP4R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine-protein phosphatase 4 regulatory subunit 3-like central" evidence="3">
        <location>
            <begin position="119"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8073824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="664" mass="78845" checksum="6A8F25754D34F7C5" modified="2022-10-12" version="1">MKPNSEILLCQMYQLAGDKWNYVDIGYTHINSKNELILLQKDTSQEVIVVPIKQENEFWFDQEEAIQFHFNKEYALSFQSREGALGVWARIQNILVENEAEDQDSISLTPVSESNLESILETMNNMIAYGSQSKQMLQNYLINKKDYFDKLIKLFQQLEKDNNINLLQTMCQIVKNIVTVAEHELFQMILNDQNYLFIFGALEYDYEMNKKNFVPHRQYLEKHAQFLQVVQIKSKERLKTIHFIYRLQYLRDCGLAYYIDECQLMFIKIVLTCYVDLFKYIENSKDFLIEVIDQLRNFNFLALRFLSEICSVFKEFPDLNKVVIYQKLSEYGLYEIIEDYINDSLKGFEKYKAKLKKLKLKISDDIFIKIPNMILELLIVCLQHCPINFRQYVISEHQQVLKYPLFNIIVENAFQNELYMETLKLLIDNNQEEQNETMDLFLIQFYPKITNQISHLSTKEYKQQFLEISLGLFRAMKPQVKEAIIINQVILKIGFILQENQKLLQTKCLQIIKLICLSRDDDINNEIIMIIPNLINVILSYKGLRENLIFSQQLEIIKIIYEGINQKLIQSLEDELKKRENQTNYQRIHDIFKNLKNNCMKQQFSSQTQSQSQMQSRYNVVDDEMDLFKSVQGHSNNTHIVKQLKKQIDQDEEVDLISKKIKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLF0</accession>
    <name>A0A8S1MLF0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0590161</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLF0"/>
    <dbReference type="OMA" id="VTQIMAI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="22"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8078093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="451" mass="51849" checksum="48A4D6F049545743" modified="2022-10-12" version="1">MDEAVVLKQKKLIAGMVIAGKYKLLEKIGAGSFGMVFKSQFLKNGDLVAAKFEKRDDSQKGVSLLIREIKVLSEVNGLKGFPQIKFYGRDENYNFFMETYFGLNLEQLLRKCGNKFSLHTTLRIGIQIIERLQAFHEKNLIHRDIKPENFTISRQDATQIMAIDFGLAKYYRDTAGKHIPFVNNKGLIGTARYASINALLGNEQSRRDDIEAIAYVLIYFHLGELPWQNIQVTSKEEKYKQILILKQNNELDKYTDKIPKCLMKMLKISKSYEFNQTPDYMGLTKLLQDELTIDGKMDWEHLLETNNDKLSVSIDIDVIENDNQFNDLQDDKQQEVLNSMQKIAQQQTKFIDGRLNNSRNNIIFLKVPKNSIVLNDAQSPAGTIKSFNTSKLNHYCGSNINFNTQKQFSDLRVKSCENFQYNDDDNYDADSDDQPCILFKNLVVGRSSSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MT58</accession>
    <name>A0A8S1MT58_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0700105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MT58"/>
    <dbReference type="OMA" id="VWEENAK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="107"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="264"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="456" mass="54195" checksum="5EEE7F30848CC366" modified="2022-10-12" version="1">MQSHVNLQKYESYELQKPSITIFEQSTQMNILTDEQIDESDSEPEKTIQETQNQNLLLSKYQHNNTIFEDENEEGVWEENAKEVNAPKPFLMSINFQNISLYRFPTLEQEKNLRAEINNIEINNNVIYYQIETKSSQLGYTVKVKRRYNDFKFLLRELTIRFPQLLLPYLPIGIGVGKLNNRSVEERKRLLQNVINIITMHQELILSSSVRNFFEEGDQQEFENKRKQGQFNMKGGLAQNLQTVIDKSIKSIGQLKNWWNGTNQNKKCEELEQIQLELLKLQNQQAKYQKLLSYIQALQKKQQKIKDLIAQQDSQKTQLLIETKLHDCILDYAKNLDFQTNTLEDKFDQYYQQQIWQLMNVIEFIDYADIYFKQLVKYIEDLNEQLTQVAPNQQTHQTIKDEINKYLSKAKDQFCNKKDGIISRYIGIQQKGFTDYYISQQQEIWNYAQEMKNSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MU38</accession>
    <name>A0A8S1MU38_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0600055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MU38"/>
    <dbReference type="OMA" id="FMEINGQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="225"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8078454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="266" mass="31305" checksum="156A96DB467CE121" modified="2022-10-12" version="1">MQNIKKSKYRLKDVIEGKTSNDEKSAQKFVQFSHIKKNVSEFNELKSTLQDRKVTFAKFAFRQQELIDDKKLGPVLKGICTRTRIGSMQITELIPKFDLYKYLKQAHLEPSKRVLRRYAHWEWDQEQEEKKEVPPSYKFTSKTEFEYKKKLEMKDAEKRLRKDIIKKWASSLPDVAGLTAFGKQFTMQSSPLRSTKSTFSKTHNNFSPNPRSESLLFVSSLIHELDEIKQDNLNIRKKLRGTQQNLQKIIEKQQQQKVANMEINGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MVT4</accession>
    <name>A0A8S1MVT4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0610034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8078924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MVT4"/>
    <dbReference type="OMA" id="GQKQNRN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8078924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="100" mass="11710" checksum="7CC9F1B9740E5507" modified="2022-10-12" version="1">MGCVGQKQNRNQQQNKFQLHRQSISISHSNRKMCIESPFVTDPKQQIQLYRSIDITKDEEFILKSDKKFISKNQESPTTTPKFNQNLQFAIGNRKNKIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWE6</accession>
    <name>A0A8S1MWE6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0650181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWE6"/>
    <dbReference type="OMA" id="CQKITLS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="297" mass="34591" checksum="D73CF2645C82DE44" modified="2022-10-12" version="1">MSNIEKQIQKQRKLSNDVEYTYNSTEIFTNSSFFDEERSQDFQDDNSSVEEDFQDNWEMSSICTENSSNPNSIEENQTIKRITKRPNPVMPIRVEPLIDNRAFIDASKSINKNSNSGKGIAINVLGHGSICKQSKKDKKQQKLLSKQYLGNNNNNSQQHQNQNQQNNNQSDQLQGHQLQQRGSVQENKQINQCQKVTLSYLLMEREVEKQAQIRAINFIEKYIKQQQQVEDVIQMIQETYKILLIPDLQMQQLAAVLSQEERDFQLNLSLLRLNLNKNIHPLAKKAESKIKQHFQLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MYK5</accession>
    <name>A0A8S1MYK5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cilia- and flagella-associated protein 69 ARM repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0690153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MYK5"/>
    <dbReference type="OMA" id="QRFGVKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048732">
        <property type="entry name" value="CFA69"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048733">
        <property type="entry name" value="CFA69_ARM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14716">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14716:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21049">
        <property type="entry name" value="CFA69_ARM_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cilia- and flagella-associated protein 69 ARM repeats" evidence="2">
        <location>
            <begin position="25"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="680"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="680"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="844" mass="98046" checksum="0DFC634845C9C78C" modified="2022-10-12" version="1">MSTHTKLGFSRSLKEVSPPQKENLSIQQVLEMFHSPYTEQLTDRKSEAIVRFCRQRSGGFLLEEMQELIEILRLSIAENNQLMQPAIQKICETASVPFIKLRTSDQLKFVPKLSEFLDTLKPILYSPQQESSSQQPELRMPVIKFLKCFAEEGISEIMKEEASFEQQKKQTELSPLQHLLKNGTRNLRALNQSNLIENIIFTMQHYSKQYEYLLPLLELISSCILYRDLAAKFSNFGILKDIVYVIFECEDFRSYIVKSCFEIIWNTIEAVGVASIKLFAVEDIISGLKKLFENIMKQGYKLEDKCLRNEILILLNYLMRDEKALQYFVDKPQAHSIQQQTNFLEVLLFYATIDEVTFYNEPIRTNQLKAFFGTTSEDLEFKKLIWSGILTAVQSGNQAVLEAVKESPFIVTLLLYIDPLANSYAVNRWSPPQKKEIQLHCLGILGNAIVYLKDDFYEKNGLFCLTKFLTNTQELDHKERCLKAFNNASEFEQNYKIKICDEGVMDNLIEFLQNENDNDNPLKIKELCFSIISNLCKECNKNKKLFRQKGAVELMVNALKNPNLGTSARYALYAVSVLDCLWNTILGNRKSEAIFLDSEGLYVLLEFLETCDDMHKKLTLSCLSYLMENPKAIPYFCDWNSPKTMINSTQLLVKIYVAEDQRFGVKYSDGIVMNKLRPLNPQNDPSLRSTNQQGDHKTKHLKSLRIQQEDLESDVGSEAYLVRRITEKSAEYDLRAIVFAILYRTGFDKNELLPNEKQMIEVIQLYPHFKIGEIWREIKYDLEQDKIKPTSDDYHWLMTSIEESEELVMNCMNTQGLIAREFRKVQEEELTKFYDIIRSNKLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0L1</accession>
    <name>A0A8S1N0L1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Charged multivesicular body protein 1a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0690021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8082923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0L1"/>
    <dbReference type="OMA" id="QQITMVM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="23572" checksum="C9E36871C33F0B88" modified="2022-10-12" version="1">MGNDQPKEQAPKPTIDDAILDMKLASKRFTNESKKCEKEKEKLMNQAKATLKKNNEEGAKMFLTSAMNKQREAENLQKMAHKMDYLQGVIKTNTNSTKLVDALAKISPIMNEQIQNMPLEKVYQNMNQFEKAMDEMQVQGNIMTGMMNQNNDIGQNLAIEQMMGQLKQEIHSEVANDLNANPNVLFKELNQQNPQQNVQAISNNQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0X2</accession>
    <name>A0A8S1N0X2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0760179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0X2"/>
    <dbReference type="OMA" id="CTVRTRT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8086418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="232" mass="27968" checksum="38144F1FCF600AD5" modified="2022-10-12" version="1">MAIYFPEESDRINKKAEQTKQKILEMRKIAQEQFGIVFPDSNPFQIKTFEQRDSIKELDENIRMYKKIQKNNDRVFIDFEKKNQYMFDQNSFKTLQFIKSQKQVSEEKIETSCQSYQSLVNRTLKSVLSQRFDKATNVSKKSSPRTIFQKDRSCTVRTRTITLTKRKTMNPQKVEFEGLINEQRRLLQTSHLKRYHKDKLQQDFQNLLAELNNIKIHKEETIIEKYKKKYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N873</accession>
    <name>A0A8S1N873_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0760074</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N873"/>
    <dbReference type="OMA" id="VNRTHFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000574">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="288"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="547"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="81"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="220"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="618"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="224"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="618"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8086236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="640" mass="73965" checksum="93EB0B655E7E420E" modified="2022-10-12" version="1">MSQSTAKNLRDLLIKQQLNSKIKFKGEPVNKIQPTRKQSGNWDKKVDISQCQCNQKNCNICIKKRVVINASPLLKVKVSTKNQPCETDSKREIKAGSSVSAHQSPNREKQYQMQQMFLQKQLQSHLHQLQESRPQSRQIPKEQVKKPQFYHNMDNFLNSYYQKSNLSNSQLYENSYNVKSKAIPKRVESKSHVLSNTQTSFKLKLTQQGPKFSAIINSGLVESAPNSPNNHESSNKSKLTPNDSISKNKINQNDSKSSELRRKSTGEISDKTQILSLKIEEQVNRTHFKFLYVIGKGGFGRVWRVEMKKNKKLFALKEMSKAKVIQKRSVNSVLNEKYLLEHLHHPFLVNMWYAFQDRENLFLIIDLLTGGDLRFHLGKMRRFSEAQTKFFVACILLALTYLHQNGIIHRDLKPENLVLDQDGYMRLTDLGIARMNKGNNAGDTSGTPGYMAPEVMCRMDHSVVADYYALGVITYELMLGRRPYNGRTRQDIREQILAKQVQVKKEELPLGWSEDSLDFVNQLILRKPERRLGSNGIEEILHHSWLKGYPWDDLLKKKIKALYVPGAVDDNFDFQNQISEETQLNDDQQLENQGLLRRDTVQSLFDGYNFDVELKNIQHQQTQKNQSQRKSQPVSPEKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEY1</accession>
    <name>A0A8S1NEY1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0870160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEY1"/>
    <dbReference type="OMA" id="LAYNQYA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LITAF" evidence="6">
        <location>
            <begin position="56"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8091160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="142" mass="16044" checksum="D33683C9056CCB18" modified="2022-10-12" version="1">MSNNNKHFPLPQTFDQENIPTISKVQYPSFELQDQNQLAYNQYAQPIQQGFPIQYTSTYHNSQIPIQSNFPVVVNCYYCQKQIQTIVSYEAGAGAYLVGGVLAALGLWLGCCLIPCYLKDCQDAVHFCSQCSAQLGRKKFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NI74</accession>
    <name>A0A8S1NI74_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0830018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NI74"/>
    <dbReference type="OMA" id="RYNYSKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="260" mass="30693" checksum="827F1178347ABB3E" modified="2022-10-12" version="1">MELLNYAATIIQKTFRGFLARQAVKQIRRINYFSSFDHHNEYGFCIQPQRSKQSTRNTQSLIQPTKRMIRSSVYIQKCYRGYLSRKYVCNFYKLKQIVLFLEGHIHNFLRKRIQQRKINIKQLMAQCYNFQTQCKSIAKGRYHYSKYMNQKQFDEINGIIFTSFLHLMIPNFEKLNSEQKSHFVDQILGVVQSNPPKKTMPTQISSKTPLKQRHIIQNVSVLSSEVMTSNGSCTSSHKSKSNLTNEEFKSILQRQNKKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKG0</accession>
    <name>A0A8S1NKG0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbonic anhydrase</fullName>
            <ecNumber evidence="3">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0870046</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8090936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKG0"/>
    <dbReference type="OMA" id="HMVHMNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00326">
        <property type="entry name" value="alpha_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF208">
        <property type="entry name" value="CARBONIC ANHYDRASE XA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2 3" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035763944" description="Carbonic anhydrase" evidence="4">
        <location>
            <begin position="16"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="5">
        <location>
            <begin position="46"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8090936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="319" mass="35865" checksum="A911CB0B83C9161A" modified="2022-10-12" version="1">MIFGFFALLSTFISAETTTNEVTRDYCSLPDPIYADGVSAADFGATGWSYTNAANWADFVVTCGGSFQSPIDIITSGTTQEAVQPQINYQSKDAAVGYEEANKPYTKEYEGEYSEIEVTDASGSIIRYYAKQFHIHTPSEHTIDGKYYDLEIHFVHQAIQDDEQDCNKVKNKLTVFGLMFQESQSAVDYDVFKPWFDSNATGEVESFDLNDFFQKMSDTTYYHYNGSLTTLPCSQTVNWVVFQQPLPISSTQLKQFQDFLSDSTLFPIKHNNRPIQHLNGRKILKGTAITASVSEIPDVEASFAQILVMAIMIMNILIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKX5</accession>
    <name>A0A8S1NKX5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0910120</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKX5"/>
    <dbReference type="OMA" id="CRPSIDK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001624">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="55"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="133"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="182"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8092772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="397" mass="46395" checksum="EBCDCE2F7BF4E273" modified="2022-10-12" version="1">MNKIVIKKKEDMNQKVFQQLNDQVPTQYKNQLLCQIDPQGSQRDNNYQKNEKDFQIIEQCLGSGAYGSVQLVRDVNSRIYYARKTISKSKLKAQESIDNLKREISIQKKLCHPNILKLCYCYEDQLSVLLILEYAELGSLFSLIKRKSRLQEREAYQYFSQLLSGLEYMHKMNIVHRDIKPENLLITKSGELKIGDFGWATQMPNYHKAFCGTTEYMSPEMIQSQITDYKTDIWSLGVLLYEMVQGKTPFQGMTFLEKSQKILSRKEVEFEFDVSDECKSLINSLLQHSIHCRPSIDKIKNHQWMLSQGQDRKGSIRSSSMISIKSTHYTSEVLESQSKIYNPQFGQKSSIQEWEAMKFRSLQFQFQRQQDQQQPQNNYQNKGFLTKALIALGCINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWE6</accession>
    <name>A0A8S1NWE6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0940075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWE6"/>
    <dbReference type="OMA" id="CIDNKIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8093825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="39240" checksum="433C5FCA06C96DBE" modified="2022-10-12" version="1">MKQYQKENRRPNQLDIHNKTPSRLQTTRLLRVHSPSTSQSPFRCIDNKIAVVNYLPPSTMPKSPTSQITYFKRKKSKQDDMIDETISKLSFLEKKIAHIMEKNDQLRSQLKKTETDSVESYSYYLSGPTRSNKQSNCISQHTLQSYVNSVKHTIHTNHSPIESSRLKDKMIGHYRSSSKTPSVGKFKKSNITIREDVQNGRGRKMYENGFVYDGDWVDGMRHGNGTLKDFNQNIVYQGQWQNDQFSGRGRFINQDYQEDEFKLLNFKQFKLVQHQFTSYEGEFRNGQFHGQGMMTFNCEGEEFKFVGGFALDAFHGVGSLSQDDKIILQGKWIYGCFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWM4</accession>
    <name>A0A8S1NWM4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0960173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="SYDQEGQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2284" mass="262331" checksum="41366BA6BA263A03" modified="2022-10-12" version="1">MNSCVGSKIIQRGELKNGKRVNRWDILYEEEQIGGGFYDEEGDKNGKWIDINDFFSENSQVIITGEYKNGKKIGRWDILYRDNVYQQFKQIGGGSYDEAGDGIKIGIWKEISDWFWANSQVIITGEYKNGKKIGRWDIFYRMNVYQQFKQMXNGQEIFRSGENSKFIHMGEFNNGKKVGPWQIWFKENETYKQIGGGSYDQEGQEIKIGKWIVLGDRFEKSKQVTFVGEYQNGKKVGRWDIWFNDCKENNKIGGGSYDDDGNGIKIGEWIDLNQQFDQRHQITYNGEYKNGQKVGLWKISKRENNILKLHGCINYDLNGQEIFRSGENSKFIHMGEFNNGKKVGPWQIWFKENETYKQIGGGSYDQEGQEIKIGKWIVLGDRFEKSKQVTFVGEYQNGKKVGRWDIWFNDCKENNKIGGGSYDDDGNGIKIGEWIDLNQQFDQRHQITYNGEYKNGQKVGLWKISKRENNILKLHGCINYDLNGQEIFRSGENSKFIHMGEFNNGKKVGPWQIWFKENETYKQIGGGSYDQEGQEIKIGKWIVLGDRFEKSKQVTFVGEYQNGKKVGRWDIWFNDCKENNKIGGGSYDDDGNGIKIGEWIDLNQQFDQRHQITYNGEYKNGQKVGLWKISKRENNILKLHGCINYDLNGQEIFRSGENSKFIHMGEFNNGKKVGPWQIWFKENETYKHIGGGSYDEQGNGTKIGKWIELDDKFDNKIQILYVGEYENGQKVGRWNNVWYEKYKIGGGSYDEQGNGTKVGKWIDSDDKFEYKKQIIYIGQYENGQKVGNWDIVWFCGQSIGGGSYDQNGYGIKVGQWIDLDDGFDQSKQVTYNGEYKNGKKVGRWNINWSKGQQIGGGFYDEGGDEIKQGQWIEMIDDFKFDLQVTSDGEYKNGQKVGLWKISKRENNILKLHGCINYDLNGQEIFRSGENSKFIHMGEFNNGKKVGPWQIWFKENETYKQIGGGSYDQEGQEIKIGEWIDLNQEFDQRHQITYNGEYKNGQKVGLWKISKRENNILKLHGCINYDLNGQEIFRSGENSKFIHMGEFNNGKKVGPWQIWFKENETYNQIGGGSYDQEGQEIKIGKWIVLGDHFEKQKQVTFVGEYNYGQKVGRWDIWFKQYGWDKENNQIGGGSYDNGGNGVKVGIWKELDDKQQEITYMGEYKNGKKVGRWDSSLLNEVIGGGSYDEDGNNNKIGQWTEISGDFLFPFQITYNGEYKNGKKVGKWDIWFKDCKGNNKIGGGSYDDEGNGIKVGDWIDINQEFDQMHQITYNGEYKNGQKVGLWKISKRENNILKLHGCTYYDLIGQEIFRSGEYQTFIHMGEFNNGKKVGPWKIWFKENETYNQIGGGSYDQEGQEIKIGKWIVLGDRFEKSKQVTFVGEYQNGKKVGRWDIWFNDCKENNKIGGGSYDDDGNGIKIGEWIDLNQQFDQRHQITYNGEYKNGQKVGLWKISKRENNILKLHGCIYYDLNGQEIFRSGENSKFIHMGEFNNGKKVGPWQIWFKENETYKQIGGGSYDQEGQEIKIGKWIVLGDHFEKQKQVTFVGEYNYGQKVGRWDIWFKQYGEDKENNQMQYGGGQYDNGGNGVKVGMWKELDNKQQEITYIGEYKNGKKVGRWDSCLLNEVIGGGSYDEDGNNNKIGQWTEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKNSQKVGRWDVFLREQNGKSLKHMQQSFNNLLNIISGNGSFDIGGKGINIVKWYSQEDGLKNKNIITINCMYKNDYLEEVWMKLEVDHQNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYQ0</accession>
    <name>A0A8S1NYQ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0910026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYQ0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="161" mass="20173" checksum="C77443DBA03F6ADC" modified="2022-10-12" version="1">MEIILFQAFFNQQLRRFKKEYKYNQNHFTQMQLLLIANDLQYERYYIGLKQSEIRNELFRLSEKERLLREKGYQDRFRLAWKKCQCGNDWTFMHLTLYDSKLNEEFEKFEEELESYLLSGSQIYLMQFYQDYMCEKIQILNEKFYELIQKNYKPSEEMEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZY0</accession>
    <name>A0A8S1NZY0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1010114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KFAFILM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="932"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1131"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1246"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1500"/>
            <end position="1517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1562" mass="185364" checksum="8D10125281F39E61" modified="2022-10-12" version="1">MQKLYLLFWPSYSKAVYSDEVYLNICGSLFSWITFQKPLEIINSNPLNILISIIYTFLHLTMFIVFSFSLYNLQVISKIFTFYISYFQLVILFHTEIHHLNQQYDLAITFTLGILLYINYLIIVYCSRNYFIISQQHMLRRYSFYNYLIVLADFLYFITFPLCTDYVSKYSLLLLSFIYFIDAIIMQPYCIKINLIYIGATGVLLMSVIIRIVLWTESLYVQFYTITLIVPLCSHVFMKIYQSISYNILQQKLSISLILHLGEIINHYNQTSIQKSVGDYIIKKFIDVETLNSSTILQLLEYHLKNLEKTDDLEEIQLYRILIKHEIQGTYLQSLLETKRFQIQNRKHSLFFKTIGFLISKKYDQQIQDLYSGENKSSQNYHFQIIKIKETDNLFQNCLGLFTNLLEQKMYIWNQLQQGYDKIENTVIDLTKMYKILNKLKDQIHSMLQSSEMELILKMQTLKRFNVVELRLLSLYYSFLLNDYQQTKIIEIYIEELIRIENSQTNSSFLNFNILNNDLIIISSSILEQTGKMIKVNTEEINRFFGYEENNKKQNCTLINLYMPKFISQDHDKYVQKFINKGNSTLYNKGKQVFCQDYEGFIFPIILSFLHINEKSEDFILTTSLQKVKSNQDYILFDQNGKILGISKYVYSMLTYNQTHNSSTQSMQSQIEQSIYECYIQFWIPNIDTLIQNSNFDHDKYSINKIILELSILTNFTQYLYYFKQYQSINPIFSKEASYFEEFLQYFKQQLSNHSFMVSLIQFEVQISMHQLPTEQFYYICSMSKQYKDFQSMLSFDQETSTYNQDEIIKQQHIHAQTPIIQIQPKFITEIEKITDVLPQTIRIIEKQLLTKEIIQDRKDELILSNIDSQQIMNKCNSSLQFEKQKNDVIIEFDDQKISQSSRQSKSSNSQVQDQIRELQKNKGLCVSIKKIWIITTFLYISIYLLVFVMLSILSSQNQLLQYDIELVRIPDKFNRLYCSFATIGQMDLERSLLGIDYGEYLSYRKINHGKIIRNEMESMMINLKNRFSILENEQRLQNLTVRILNQFSYTEEELTMIQFDMIADSYTEQLFQYIIQGLNYSTSNRLSLIDQYYKLQFIKANLINQITSSQNLIDEIIVEIQISQETTITILYALLSFKLCFVLLCILCLIQFWKKPFYIIQLQVELLSHLSEEQIKLSINSAKQAKQLISHPYKWKRTNYLFDCYHKTIFKDQQCILSKIQNNIATKKTKQNSRLLDYQFNHLGIYLKNCMYLILLLSFIISSFFYMKYGIDSTQSEILLTIQYVQFKQDLDSLVILSQLLKTNSILSGRVRELGFLDLNNQLQDPDKYFHTLELELIEKFQIKQDKAQQVNQIIYQDIINSKKISSNDKEILQTLYQDDLCSVMPDQLPFCNFTNNQFQYFPDYPMPRDEDNNREIFRNGINGIFQKLLVILKSYYQLELQGHKNTNKTETEYFLSTPEFKHYILEYFFDINKAVVKFYTTILESTTDILQNDFKNALFYYVIFGLSVLIVQGTLQIQNLSKIQELVSASKFAFILMPLECLNENKCLAIIKQIIKQYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1F0</accession>
    <name>A0A8S1P1F0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1010160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1F0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="26798" checksum="44F28D3A0BD5B93B" modified="2022-10-12" version="1">MFKLNPKKVLIKTPTIYDYQANLPVETRKAIVLHVMNKNPKLKIKGGNSMTNPYKIPYELAIADQIDQMEKYKNSILWSPRMTPKKFTFAFIYPMGFALVLLWYIHNVAIPRRMVEMKRKYGYVYPELESKGWLDGWWDYEQEETEQIFDIINMEFKKTENKVKNKQYTDFTISDLQNFGNKPTYENSEQTSNINKSGKQIMQVKEKQKIQSQLEKILELQSKATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1L2</accession>
    <name>A0A8S1P1L2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0960038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1L2"/>
    <dbReference type="OMA" id="VANTHYC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="469"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="310"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8094474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="736" mass="86935" checksum="6CD63163FA6EF403" modified="2022-10-12" version="1">MNLKIYCKQFQKLFLIDSTPNMNMKQVIDQITQFFGCNVSTLTTASINDKILSSDLTLEELKIGQDCQITLFLQYCEHIKFQLKHNKKYIQTLEINIYNSIQEVEQLLANYFKIEYDVKIIVNDQKISKTLPFSQLDLIKTHEYEINVYLQIEMNKNQQKLLDFSVFDKISDIKQKIIEQMQIKGEFQIFYNFGIQLDTIENMEETIYSLQIPNYQTLKIQSLQQINLKYNVNNFSEQYKIKINQKLIDFINQLRKKHKYSTNYINNIKCNEQLQTLNTISLKEIKQYQIDIEENKDETINITIINYFDLANRELKIYNINQNLDELKNEIKQKNNNCQNVEFIIDERFLETDSKTFQQVGFVANTHYCLQYKCINYDNQHQKFSQNITQLDKHSQTFICNNQQNLIGNNQQQIIALSDSKIQSESDQIEGSNHFERSSGLYNNQFSKNKYHNSSNITEKSLLNHMSLYQEELRTEDQNIITITIIMKLNDQIFEQNQQIKENQYSILSQTQSYKDLLAKGKTNITLKFEGKIIDVDKSFKDLGISNNSTIEVIIQSDDILLSQINQQNQNDEESSQINEKVKIQFQFQKDGIQRNEFKIYNKETIKFSLIRKQLLMQYQLPENMEIMYKDKKVDLNQNISSILQEGEFLFILKGQENLINKKLVNLNLILIDGPTENQSIQVDQYIEISNLYNLVEQKLNQTNLKLYYEGTEISNQKETYITLKDDGENEIEVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P3T6</accession>
    <name>A0A8S1P3T6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1040173</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P3T6"/>
    <dbReference type="OMA" id="CYYLAYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053215">
        <property type="entry name" value="TKL_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45756">
        <property type="entry name" value="PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45756:SF1">
        <property type="entry name" value="PROTEIN KINASE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035828067" description="TNFR-Cys domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1151"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="3">
        <location>
            <begin position="396"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8097678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1206" mass="139557" checksum="92CDBDE419D01BF3" modified="2022-10-12" version="1">MIVLLFVTFKLIQSCKSLFNQLGEYHCETCDTNQDFCSSCPSTRNLKIVVEKGANVNVCHCKQNFVDIGGQECLQCPSNCVDCDPNDPSKCISCGNEELTYRVLGSNQTCICKEGYIEVWYTYNCQNIQFKQQILQFQYILSLEQYVNGLQNKYSYSQITIQGLDYMIERGIVNDGINQFQLISKLESNKIISTTTPYPQGQAYMQKFDNLFAFGIMLNHESTMIWAANVNYINYNQYSGIRILNQSIWEFVIFQFLLQKYEGNVFLQIALLENNNIKNEKQNRLLEIKEGKMALCYYLAYELNNFLLESQLDEIINDILYILLQRPSNSLDYFYNGQELIIQKCQPNYILKNKECQCDNENGYYIYNNICQLQCPDDCITCTKEVCLLADHSIRCQENYYLDIQKNICLQCYQTCQTCNNEKECTKLIAYNCLCLKCMDGYQLDEFYQCIECSQNCKSCHLQDQCNICKDGYYLSDNQCFQCNENCILCQDLNYCIKSQKGYYIENGIALKCINNCLDFLNECQCQDCFDKQYRNDIDLFCYPCPVDCQTCVNQQTCINCVSNSFYLDNSECKECQFPCLTCLSQTKCLSCIDEQHYYIKEHQNCAQCPTPCKTCYYNENPLCTSCLQNHYLLNQQCYECPLYCKDACIFDTIKNFIQCEQCQKGFYPTYINIPFECKRCKEYCTICINEESCLECSLGYILNNGKCQSCSQLYQNQCISCNNQFCLQCQNGYKLNNGNCIEDKIIEVPICLVNKQYYNTISNSCDSCIQNCIICKEQQTCIKCEETYYEKSEICIQCKFPCNTCLNDQFCLTISNQLYYIDNRITELNQINSFRCKSPCNKCYSNEECIDCIDGFYFENNNKCLQCDSMCKTCKNMSTFCTSCNLGFLLLNGVCYQCLDQNCQNCNQFCQSCNHFKDDLYKCQSCLIGYYLINQECHQCNSICQSCSQSSNQCSSCFEGYFLTSEMKCEKCNLGCKSCQVQTKSCILCEDGYYLSEIKERKCQQCVLPCLKCTTLTQCTLCENGYYLNNEMCERCMENCLTCSSSNNCLICQDSYQLVDLKCIKCPNNCITCNEGQCLQCEQEYILQNDGYCHKVSVICNINQCQKCLDVNLCLSCSKGYTLNTKNDECKIENQILDSESTDKDDSGTIFIIISAIFSLISFTLIYLIIKCRKIQIKIKLIERTQPKFPELPVTISEFPADEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P4K3</accession>
    <name>A0A8S1P4K3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1050096</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P4K3"/>
    <dbReference type="OMA" id="CNENEYD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000274">
        <property type="entry name" value="ras-related protein RABA5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8097908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="218" mass="24895" checksum="7D833F4C9296369D" modified="2022-10-12" version="1">MTRCNDNEYDFLFKIILIGDSGVGKTNILQRFLKNEFILDSKPTIGVEFSTKTINVQNKTVKCQIWDTAGQERYRAITNAYYRGAVGAFVCYDVTREITFRNTEKWLSEIKEYAPKNIVIMMIGNKIDQANNRIVRSGEAAKICEQHQIGYIETSAQNGQNVEEAFSNLVSEIFNSMSSIQQISVEQLQGDKLTFQDDSLSKNAKKKNSRKEKEKCCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8M9</accession>
    <name>A0A8S1P8M9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1090115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8M9"/>
    <dbReference type="OMA" id="QSIEFEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="1">
        <location>
            <begin position="2"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1152" mass="135510" checksum="C76873E66A24E3A5" modified="2022-10-12" version="1">MQVLINSIYESAGDTRIELINLMQEDIKVKKGIVLGDLSRIFIMINQMLIDTNKQVILATHGLLSSLIDYLLPAELEGGLKHFENQLIHNLADSDRQIRKSTKSIFLKFIAKTRNSEQVLELLSDSFKNKNFYIRERSINLIPDIFIADKSIFCNRTGINEVRKVIESIIIHLNDVNESVRKASKKVIQNLKNIENFELVYRRLAVQHQNVVDKIQQEMPPKVDKFIELNFKIQEHKDPLFDQFVPFEFIAKTNLTIDDFPQDLKTQLYFGFLPQEILQDTLNQKDLTIREKGITELNMQFFEDEGESQNYEQKTVDKIVKGSFMPSFFKYISSLFFSEDKKIILMILNIIGKIMQVPGVQVNVYLPLCLPGLVQCLKDERVIIRQKALFLLRKLTNYISKKQYFQNLIYFLEQKMDNWHQKEELINLVSYLILMIHPSEQWFYGIDWKEMNIKQEKQIEELSFNLREVDFKKSLITIASYLNDSIAKVKYASLECLSLLAYLDKKHLEYLHEILNNKVYEKLNDKVDNGIVYFINDNGNLELPQFNIKKLEEQKIQSQIKLSMVQSVQSEPKPQTPVESEKSEESRYKITYYYDKFQMSLNDKIIKSNASIDSLFASEHKNNQGTKYDMIIPLGQFQQTKANEQFKLVNSNLYLEKEKVDDNPYEDEIMHSDDDELQSIEFEYPTRTLKYRGHAPEQITKSKYQPIDDIKSRVFHTIKQLNEDINVDLQIPKVIKANVGAYSGYKYEDEEEADDEEIFIDTKLIKQQHRQTQPTQEESIINDEKVMKENFADNQIEQVQEIIKNQQLIYTPEQRYQKRLDEFLLSFEQRDNKNKGKNARKLKKKLKEIAKQEELPKVFDMPVFIDNARAETAALLINLSSLNLDLLAVSLHKFRVLLQNHLSDVYASQVPLLQIVNDVCRILSTQSDNPLYAIDYSQYKKNIPPQIVVRKIVYIALQTLSVAVQTLRSTLIQVQKMFTVCGVWLQDDNKQISQQAEQTMLDILRFCDDSQLQNILPNLYNKQSFNSKIYVGVLIEHLVIKYQREIFKSVHFNNIIQLISQMIIDQSFDVRRIGKKVFFKILTLGKYQIEEIVMQMHQQEKNQMKELMQRYDELVTDHQIGIFDQEEYMGLQVMPQKFNFEYEMIDDQIYMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PC35</accession>
    <name>A0A8S1PC35_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Methionyl/Leucyl tRNA synthetase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1130078</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PC35"/>
    <dbReference type="OMA" id="EMEITYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023457">
        <property type="entry name" value="Met-tRNA_synth_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326">
        <property type="entry name" value="METHIONYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="313"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="9">
        <location>
            <begin position="512"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8100747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="841" mass="99774" checksum="2D793644C365A1FB" modified="2022-10-12" version="1">MHSYQQQELIDLLQNHSNIAIDRQLISQLYNKCLQDHKQCTLPQFEKVYKEAIQLLSQKIANSKQQLQEEQDNLVSIDERLKNTPLINNNNYRIPMRLTLHSASVLNLQVHNKFMVGSFAFYMKIEDYYEYESDFKPSYNLQFQINQTIDILDVGDSILLALNTNTQNQMNTTIVIPTYSLQFNKNVTELLTQKENGELLEIEITYEIKVLDQKLLTLQLNQARNLVISYIKKFEDELEDYSLQLQDLRQIKIPQNNNEISNELNDGQQNQSRQEPSHNTYTIGVPDYYHKPLELQSQDCQQNPQPQERQSSIIPYLFGIILASFCNIFFNVLFELSLLMIVLIVVLKDDKTNVLTKLMILLSWIMFGLNLWMVRIMYIIIIQNLFNFFMIKQIITTPLFYVNAQPHIGHLYSMIYADAIAKYKSVKLLTGTDEHGLKVYRSAKHQPLQQYCDSISDQFKQLANSFIKYDQFVRTTDPQHIIKVQKNWNVLQNNGFIIKDTYKGYYCQVEESFVNHKTDQYLEEDNYFFKVDKDYLDTCIKNSPSRYQNELKQLIINSICISRPKQRYYWGIEVPNDNNQLIYVWLDALLGYLQEEQHHYMHVIGKDIVRFHALYWPCFLKGINNQSTTELIVHNHWIRNNMKMSKSLGNVIDPFQLLKKYNQNQIRLYFLTQGPQNMDVSFEENKLEKCWNTYIDQFNNILFRLFRPKIISPNLLLLQQHLLDDQLLQTKQSFLELHKNTKAFLDKNQFIEGYLELQKIFVLINTLIDKNQPWKMQDQFKKEQFLSFICILISMTQQILNIYVQTPNIIIPQFKEFPQNNNVQVTVDLEQDLRLKRFIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PPG0</accession>
    <name>A0A8S1PPG0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1230133</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PPG0"/>
    <dbReference type="OMA" id="DWICHST"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00065">
        <property type="entry name" value="FYVE_like_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052113">
        <property type="entry name" value="FYVE-type_Zinc_Finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39490">
        <property type="entry name" value="ARRESTIN DOMAIN-CONTAINING PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39490:SF8">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="389"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8104338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="456" mass="53736" checksum="B7B843F229EE5DE4" modified="2022-10-12" version="1">MDKQCSICNKEFSFIKQNKSKCKRCGLVVCLDCNKNEFKISSSMLKPLKKEKVCLQCKTDCLYMEEQLEQSELSWNKETELQIDLYQQSLNQEQYKQQIKNAKANENYKQNLEQIKSDLIKCFITQEMFNYSFQDWICHSTNKMKVNQIFDDIENLLVVFLYRNAEVGYSCELLQISIVLLTLMKDFAALQLLEQLYELMPNEYWPTSTQEPNLQTLVQDFQLAFKIDSTQISQIKKFMEFFSTPNTQSLFIKGLNFSCFHIIFLEFVQQMNFEVIQKYLIVVAKCCLKSFGQFGFDYKTIGKQILTKTDYKSITQIKTLLDQKLGNSQLTFSSPKGSMIRKRNSVCSIDNGSFIDTLPQIMTQQNSQREIKIEDNFIFNFQNEQNLQIIQLMKMIEQKKKELHELQIKNNALKNQTQNKKQISKQDHENYIKMIEKLSQEIQEELIQGLQLCDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSD1</accession>
    <name>A0A8S1PSD1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1300057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSD1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="634" mass="72939" checksum="6F885756B5E17AC1" modified="2022-10-12" version="1">MKLLLLTFLVVSQSQWINTYEAFVEDSLTTLDGWVIQNQFQATSDIITKCNEKTIIGGLKTFGLKTTATKFIKLPPHYKLKFNIQLWKLGEWEKEVFSIYIDGVPWEMKWGFTEGGIRLCGGPESKHSDKIFDIEFEIAHNSPTVTIILATNQQGQADIQAWGFRQVKVSFFACPSECGICHNDKAEDCKNWNQEALSWFHIDTKLEGWQLENGKDKGYECSGIVIFGGYENVGAKSTLSNSFISIAPHYKIMIKFTFWKIDLWDNDEFYIKIDDKEVRKIAFQTTDGIELCGVKSNNPGYGEKIVAIEVIQKHSEPTLKISFSSSLSKDLNQQSWGIRDFFLFAAQCTKFCDECVGNAENECTKCHSTHTLSNGKCLNKNEWYVASKEFFEEESFKKVEGWNFQELDSVSPNPPITKCSDLNLIGGYQSFGKKTTVSKKFILPKHDYIRVKIIIYKIDNWDGEELTIFIDNNELWTQFLGWNDPAQSDICGNKSNNWKERIMKVDKILTHSSAELQLDFRSTLQKEANEASWGFREFQLLYSPLNECIEVFSECNYKGLQERICENTESLTQSKIQFDIKSIKIPEGMKITGYRNLGFKGDKVEYETNQECLDTIEFSFIEKQLIGNMKYIHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTR6</accession>
    <name>A0A8S1PTR6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1310023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTR6"/>
    <dbReference type="OMA" id="KTILYDH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032273">
        <property type="term" value="P:positive regulation of protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008907">
        <property type="entry name" value="TPP/p25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12932">
        <property type="entry name" value="P25 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12932:SF9">
        <property type="entry name" value="TUBULIN POLYMERIZATION-PROMOTING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="732" mass="86611" checksum="8E5504BF5102A97D" modified="2022-10-12" version="1">MRSSSSNYSLQRDIQKIESIDLRNRSGIRNKKEENAYSRIDNKENYSIQTGKNQLFSYGAKYQHKLQMQKSKVEIPIVEIKRVDDKKQLSVIFKFYASFGDRTNIDYIRSNKVHKLMSDSQIKLQRQDQQQIDLLFVKANRNKPNMDFENFYYFLELLSEILYGENSFQILMDNHLTPLYENIISSTDLGDEETKLSQEIDPICVKLMQVVIQPITFIYNKYFPPQFKNFNLKDQQEELMLKQQIRSSLFLFLKEFEICPQFITKGSAYLLLDYIQADLNLPQICQLKKQKSKFDLNQFCCYIIKLAMMTLQSFDDSTQEQQIITPLQKLAFLFERMELSQGFNNLEIPSNNSKINWKLKVPEELIELIISEPDAIQYLNSTTQRMKIHQLKNEEFCDEQVKRINTVPTNRIRPSIQINKQFEEQCQQLFQENYTQLFNIYRQYSGISQQTGQDNELVGSKWMKFLKDANLVNINLIDKNSKLSYQIPHNEIDLIFTKACTVSQNNRTSVPIKQKKQTMNFLQFLKGLQLLTNDIHVLKTILYDHVLHLMSQGPENKMKDLLSQLMNLLKDKEIVEFLGLLHKSILVYYKHYTIGKQIMNFEQFLRFYKDFSIFPDLLTKNKIVQIFQILSKLYENQENSNIKSGNIDQHMFVESLALSALEILYNDDENLSQLEKIYFLIERLNQSDGPRIVQKQLGHTKCPSGQDWDLLYHIRKRFSWLIQQQEQVSQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PV90</accession>
    <name>A0A8S1PV90_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1320081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8106971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PV90"/>
    <dbReference type="OMA" id="HYDLKSH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="159" mass="18931" checksum="442E869F37770B17" modified="2022-10-12" version="1">MYMRYHYEPKSHIKNSDCKLNVHHSHTTSLPLLLPQQLSPVKEKSPQLSKMGEQILKQWGFFPDISRTSRKEIHTEHIKTEKKSKDKFDDLLYNLNTERANYQKNDYFQNDFNLKKSVHFNNVVTVKDIDGQVSQKEIKLFSRNKRRQRIKLENLTAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PVR1</accession>
    <name>A0A8S1PVR1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sperm-tail PG-rich repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1330033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PVR1"/>
    <dbReference type="OMA" id="RENRFQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031344">
        <property type="term" value="P:regulation of cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033602">
        <property type="entry name" value="CIMAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31508">
        <property type="entry name" value="PROTEIN PITCHFORK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31508:SF2">
        <property type="entry name" value="PROTEIN PITCHFORK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="575" mass="66608" checksum="732EC49FA8E525C6" modified="2022-10-12" version="1">MAFVFQAERGDILQQSGVSQDVKQGNAPFDSSVSRCERIKNEIIPGPGSYYQEYDDNYYGQQLSPKFMKLTNKKQQSFIFASSSKRFEQPKTFMTPGPGSYETDIIQNKPIQQQFTSQNQLEILKSLNRYQSIPSIPTDNQVYGYTEKGAHDLESNKNPEHTYSGIKQDTVGPGKYQIKDTFEQNKNKGVCWHKSKIPRLEPLISKDRNQSVGPGSYDCNRSITPIYKLCPSGNFKSKSSRTFDNTKGEKQKQFMKIYFEKQKEKLMRNHGYSDLEDEEFQYSDITTPGPGHYFGNTSTQSVSSKNSQQSIKQGYNCFGSRSKRFQEQKVHYHIGPGEYQLETEIVKQNIVQPPFYSSNTRFEEKYYGQLPGPQTYDPKITLAEKLIKKLEWTPIGNFGINESRFKEEYNQIPGPGTYELNNKVQKGINSIFKSKTKRVSSPKQKIDNFPAPGSYDVKNNTIEYELKKNEDENQELKQQKQAFGSSMPRFISRKNKHDRVQRVFEDEEEEEELEKLHNTSGLLKKKQNIQVSFNVRENRFQTKKNDTQKLGPGEYYSPNSKNWDKRSFNVLFQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PW52</accession>
    <name>A0A8S1PW52_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1330091</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PW52"/>
    <dbReference type="OMA" id="MAFRYVK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001311">
        <property type="entry name" value="Dynamin-like protein-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF233">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_59, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="5">
        <location>
            <begin position="35"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="4">
        <location>
            <begin position="566"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="522"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="528"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8107284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="686" mass="77722" checksum="F0676675DA58A595" modified="2022-10-12" version="1">MSEQKDSSLQGQLFDNLRSLITLIDQLRDLGVQEYIKLPRIAVLGSQSAGKSSLLENIVGLDFLPRGEGIVTRRPLEMRLIHKPDALKPWAVFDVCKDQKFFDFEKVREYIVELTDKATGNSKDVVPDPIVMTVYSRDCPDLTVIDLPGITRIAIKGQREDIEKVTLEMATHYCQDERTIILAVCPANQDLSTSDGLQLAIKLDPTGSRTLGVITKIDIMDQGTDCRKELLGQVIPLKLGYVGVKNRNQKDINEKVRVAVALQKETDFFAKHPLYSQMPPDIMGTKALIQKLTNILFKHIRASLPQIIREIDQKVQDCEVKLAQLGPGLPRDNKEKMQVIWNMLTEYTENLNNKLRGKFDVKQALQKDTQISGGAQVKLYLNDLYNDQAGKNYRVSREYTDREIREAIRLHQGDSIPGFPSFDSFLYLIMPQLERLREPAIDTLNEVFTYVDSLSNSILKKVFYRFPTILDAISDISSQVLTEQKNKTKVVVENLIDAELGYIFTNDQDYLTMNASIIPQEDKEDQKNAAAAPSGDNAAGTANTTTTANKPAQSGNKDTDPEKVLAQELSRRLDAYYRIVIRNLRDSVPKAIGYFLVRAAQEQMQFQLYNEIMKSQSVMGLMSEPEYITIERDTIKKTLLVLRNAQKLLKQDPQQTFQRQTSEAVMDKVKKTAAETINKTKRFLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PW66</accession>
    <name>A0A8S1PW66_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL15/eL18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1340008</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PW66"/>
    <dbReference type="OMA" id="IMGADIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="5">
        <location>
            <begin position="2"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8107485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="178" mass="19827" checksum="CF6C954866DCAAC2" modified="2022-10-12" version="1">MGVDAYKKTRVQRKVGRKVTSTNLYLKLLIKLYKFLARRTDSQFNVTILRRLQSTRTARYPISVSRLVKQINTAKDKTRTLVVVGTVTDDARLLTVPKLNVCALRFTETARKRILAAGGKTLTFDQLAQQNPTGTGTILLRGPKVREALKHFGRAAGLPGSHAKPYVSHTARRGKGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PY85</accession>
    <name>A0A8S1PY85_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1360093</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PY85"/>
    <dbReference type="OMA" id="HENWRCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013233">
        <property type="entry name" value="PIG-X/PBN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040039">
        <property type="entry name" value="PIGX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28650">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS X PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28650:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS X PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08320">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035822401" evidence="11">
        <location>
            <begin position="18"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8108267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="352" mass="41445" checksum="DB1BE749A805883C" modified="2022-10-12" version="1">MFILALFCFVSSQIIIGQLPSNINEQLTIKVDAKSIRISYCLSSDQYKNGLKKSEFNYPNPKTFTQLNHKLLLQYITSEILNEMEHKSALFYHENWRCKQYTIYDYFLEQFEISINNAKNRILTILKQEKQDASEFKQSQDWTYQLQLNSNTQQQQIQINYKNQSNGPKYHYQRKLIGAGLHQFIETKITFDKQPSQNVVIVDELDTFTYVDKDELDKLPYEAKLMTIVDIERPNELSTQHIILSKHKGTQEITYIMPYHFRYHLASDTHYKTSYVPNIPRVYLESQQGDSLIKSFFNGNYEQAQISTYEQHLTYQIPVGYLPYKNVVVMITIGITLGLALHISNLIRREKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYK0</accession>
    <name>A0A8S1PYK0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1360087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYK0"/>
    <dbReference type="OMA" id="ITANICF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="429" mass="50850" checksum="E358E15C52FA064A" modified="2022-10-12" version="1">MYLTADSALQLLHEKRNKFQVEIRRKRTENTFQQKRAIFVQNKVWDLELLAQYALEFYNRGDLQECENYLIRLNEYADSQYSANLKSINTITFLGEIATNCDYHILAIVLSIYQKIYLPECLNPYLLSQIQRFENSELIKQFVVLLHKIVKNNPLNHLVEQCDFVSYLVEISLLLDNFEQWFKIVEVLGSYIKQPSYYFKKLVVTCIKFIEYDTDLATFQIMLTMISCEKDNKNSINYLLANAGFVDLIMDKLDKNMMLSISIISKIVEESDEGTILFIQHGLLKTLSNLYFEMRENQTAFIYITANICFLKNDYVITEVLTSEIFNTILALDEQELNKNDYIYISQMLQQLIRKYSNEQLYRYLLNKTDYVYMLGHMMKQFELREVLQNAISCVIHMAHSQSEELAKLFRYDINIIEQVFEFLNIKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZ99</accession>
    <name>A0A8S1PZ99_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1370117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZ99"/>
    <dbReference type="OMA" id="SIESMKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="279" mass="32751" checksum="9CEB2FAC9DB59F4F" modified="2022-10-12" version="1">MDKEFVYNLENGNYYVLDFVNQKVQQTNSRGKFPENFIPYQSGFSNSQQRQLKLKYHNSVSYTPHQNKYFGYCQCPNEKIKHTSLSKVIISKNTSDRIEDVKQTLPVNLVNCFNKPKTPKISLTQNIYPPKYKITENNFDDNLITHLSGTIKDAEQVYKSGSIESMKYRKGTKIMDKLLKFKKNEPDQLRFTCRYLNNKKIKSPPYKVMQRYEIINKINQEQERKRAKSIDDSLVKRIPPVPNLQTLPSKIQNNFYQNIDVSLSHSIDSQKIKAFISLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4D9</accession>
    <name>A0A8S1Q4D9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1410043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q4D9"/>
    <dbReference type="OMA" id="EPEITHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="158" mass="18034" checksum="FDB319D0B9D1C6F6" modified="2022-10-12" version="1">MLSRTNKWFLKNLSRDHKPDCQDEAERIINNGGRIDPYKDQNGQACGPLRVWNNGNVPGLAMTRSIGDQIAKSVGVIEKPEIFNFVLEKMDRVILLGSDGVFEFLSQQDILDSVTPHVDRMDVESACNHLLEMAHISWVQRSNKVIDDITFILIFMQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7G6</accession>
    <name>A0A8S1Q7G6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1460150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8111203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7G6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="209" mass="24726" checksum="6FC55D67F239441C" modified="2022-10-12" version="1">MKITFTTQSNSTQNKYWGINAFDLYIGKCSINCEQCYGPGYKQCTACIKDWVVFKGECIYYNQIPPLTWEHISIVQQLNNISDNIYQIQLKSDQIDQETLKLGENEIEFDLNMPILNLEIQAQCLKNVNIYNSFQIYIRQNNQKQYLYTFSCKENLPTIIIKTHQVQKFEQIKQFLLNLTETSVELYQLVIIEYQQTQLLILSLNFIWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QED8</accession>
    <name>A0A8S1QED8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PARG catalytic Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1580075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000163">
        <property type="protein sequence ID" value="CAD8114188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QED8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004649">
        <property type="term" value="F:poly(ADP-ribose) glycohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990966">
        <property type="term" value="P:ATP generation from poly-ADP-D-ribose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046372">
        <property type="entry name" value="PARG_cat_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007724">
        <property type="entry name" value="Poly_GlycHdrlase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837">
        <property type="entry name" value="POLY ADP-RIBOSE GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837:SF0">
        <property type="entry name" value="POLY(ADP-RIBOSE) GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05028">
        <property type="entry name" value="PARG_cat_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PARG catalytic Macro" evidence="2">
        <location>
            <begin position="244"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8114188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="497" mass="59819" checksum="A4227D621CADB53D" modified="2022-10-12" version="1">MQLRVRANKQEYFDFSNISNDKHFMVQFNKIANQLIKDNQVNLINYINEFIIQQSQTKIRNNEIKQNIQKEKSNYEQGKNYNQSFIEQQIQFTRKQVFILLLMMYSNAIDKDNQNMQQSGFYQINMELIKQSQCKQSQEKLKCIHSYFINFYNRFLKQDQVLNNDQQLNQNLKQIQDLEKEIQEEEYKHSQKIQYLNQKIEMIRNQLLNNNNEQDEFVTYIRRTEFRQYNQIFNDQQYLLSYFTKNKGRCEDYQNSVLVNFADKNVGGLSLDTLNIAQEEVLMLTHPEALISMLFMKPMDDNEAILIKNVIRFNDYDGYGQTFRQKDEDYFKLIQNSSFKQNNEFSQQKEDVNKLTQNKKIIKNHILCMDALYYKNWQLQFDQVCINRELTKSYIAFSLALDYTNNENISTGKWGCGIFNGDVQLKILILLPAFLMAQQDWQQKHEQNKMLDFKNKTERILIFSSFHNNQFDDLIYSYEKCLNEKRLKILQNNNLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFV0</accession>
    <name>A0A8S1QFV0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1580054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000163">
        <property type="protein sequence ID" value="CAD8114156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFV0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="131" mass="15580" checksum="71CE42DA54D29885" modified="2022-10-12" version="1">MGFKAAMQLVEETQKQKREQREVFIIKNCNLLNSFQLNLRELQTSINKELDSFINISKFWMEDLNKVKSDQAPLNFLEELDTIMKQGKDSLLNKELVVEKINEINNLWSAKINRQLEKYCQFEEDMLSNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QG23</accession>
    <name>A0A8S1QG23_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1620023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8114929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QG23"/>
    <dbReference type="OMA" id="FECHYVT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="15140" checksum="7F0D6171F1935D94" modified="2022-10-12" version="1">MRHKSNGQKIFIDGSNLLLDDESIFSYRPRFSKHKNFYAKKPLFECHYVTPTQKHAKEILDKICHVNKKALITERPESSFQKRKEFSVDQCLTTRGSIQRKQIRIKSINRSNIQNKLSDQDWLAFVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QGQ6</accession>
    <name>A0A8S1QGQ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1610108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QGQ6"/>
    <dbReference type="OMA" id="HSIGKYS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="686" mass="80855" checksum="C765CAA5F9C63498" modified="2022-10-12" version="1">MNNIITLERNKPLKYPSNDVSMNWPSFQSCNVSQYRLHLATDRVFQHSIGKYSDTESERNEIPQNLVKLSEFQQHSQQNDRNQQLLKNENFWIAKFIVSDLTKSFLLILSFILPILYIEEYKFKSTQLKITITILMLSLEMVSIFIKLYSQILKHQVMQLTVVQIMNLIVYTICSILEDQLSYGILSIYLFVGNFQTLGKLCKLLTFLNYEYIIVFICHRQQNYFIILSCIYFSLHLFGCMWRVEYEEQNQEIISYQDGIYQSFLISLLKIDEMEIENKQLIILNVTFNAGMILYILKCLLMSSIEKSKRQLLLDSFRLYLQSQLISFKSKLVIIHYSQIFEVLEEDQTLQQEQRINKVSNYLKQKLIKQELSKVKFLSVSTLELISQNGTFIQSLPLNKLSTEIDGLYIILTGNTYVDFMGFQIKLQSQKVIELSLTEVINNQWQGRVKIEQTDSKEVLYFFIQKQILIGILQQSKEREVYQMIKDDIIFYNDTTKLNFRCYFCEQFHPSFNCSSFNIKRRFNYDQIYQERDARYSRKVTKKSRSAIFQQITYQGTSNHNVFEQSSDSFEAFSDNSSEHISSANIGVDQITKLPSTKYIYKEILQADIISDKLTEPIISSTFLRNLTTSPDNIIKRSIINNTPQQQACFQDLDSLFEIDKMVEFQDYKTKFNITNIIQILNKKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QHZ0</accession>
    <name>A0A8S1QHZ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF541 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1650082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QHZ0"/>
    <dbReference type="OMA" id="INNIECT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052022">
        <property type="entry name" value="26kDa_periplasmic_antigen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007497">
        <property type="entry name" value="SIMPL/DUF541"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34387">
        <property type="entry name" value="SLR1258 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34387:SF2">
        <property type="entry name" value="SLR1258 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04402">
        <property type="entry name" value="SIMPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035825579" description="DUF541 domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8115638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28647" checksum="2354D9C91EB060AC" modified="2022-10-12" version="1">MIRILTIAICITIIFGSFVKEQQAINNIECTYVEYKRPEIFYVTGTGSLSIEPTIASVNFAIEIQNELAEVALNTANKIQTQAFKSLQSVDTTNAGVKISTTQFQMFPHSEYDYPYNGNAQLKFQGYKVIISQKLETPNLKIVGKLIDAAINAGVTTINNVSFDIKPEQKSELKDQILQLAIKDATNKAEITLKALDMKIHSIKSISIDQSYAPRPVYEDQAVMKEVLIGAAPTKIFAQEQNLQHSVTVGFIIVPIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QI06</accession>
    <name>A0A8S1QI06_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1650092</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QI06"/>
    <dbReference type="OMA" id="IYAVCLM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="39"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8115658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="355" mass="42074" checksum="843E7062A2867A18" modified="2022-10-12" version="1">MEWSRSREREKIKKQREKEETKKEKHYKLQKDEIFNQRYLFVQAISDGTFGRVVRVFDGGENDFKAIKIIKSVKRHIQSAKIEYGILRTIHQQNQYHPGNEKIVRAYEAFSHKDNYCIVFEDLGLSLYDFMRGKYFGGFELKAVQEILYDCLQGLDFMHTSGYTHTDLKPENILLIDKQQKGKKEGHYHVKIIDLGGAVFQTDCHSQLINTRQYRAPEVILRNKWTNLSDMWCMGCIAVELFTGQQLFKPKGNDFYHLAMIEKHCGPIPIEMIQQCRNEAREYFNEYQLNDSFLKWPDDQFMVQELNLMKVFKALIPQNQQELLNLIENLIVIEPLKRLTPKQALEHEFFKLTFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QIG0</accession>
    <name>A0A8S1QIG0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1590061</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000164">
        <property type="protein sequence ID" value="CAD8114330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QIG0"/>
    <dbReference type="OMA" id="FAYYFAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="213"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8114330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="528" mass="62402" checksum="7F7E935256B19FB4" modified="2022-10-12" version="1">MNQSIIHFNEERLEDTYIQLELKKTTNEYMEFKCRRTTNDYNYIIRRFTKQNINNERFLKLRKIKLYNILPALDLYLQENSMASPDYQEYSLENNLEHMDNNEKVFVASLVGITLQELCKRKLPFLIKPDKVIIIGKHVCFQQIDFEFGRIITPEESFQSFIDLISWMGLECNGDDFQETIDYFLVQQNVNIGLTESLPYVEILQQIFQFANDQSMVDDKANSFVYVFDTPDFLKTIPGISNRTVMKSAKLDIADNKDYLILCSQRELEIMENFRKYEPFAYYFAYFRIQDQLYLFMELYPTDLSKTFENSEIYDRNVKDLCCQLAFAIRQLHKHRILHRDLKPANLFLSHEDLDRAQLKIADFDHSTTIEDAPEYCNQILTGLTSSTNYEPPETGQSNYTYTSDIWQIGMIFYQIANQGIYPVDHSSLFWTEDRYKEECTYEKIKQTLADKNIEDDFIKLIADCLSFNPQDRPNADTLITRLEQCFEDFVFENEETLQSTHQINLIESFGQLLSKSQPLRFTKSGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJ83</accession>
    <name>A0A8S1QJ83_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1610009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJ83"/>
    <dbReference type="OMA" id="FNCHLAD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="372" mass="43358" checksum="9875A02346BD320A" modified="2022-10-12" version="1">MASHTISKDMETDKYFLIDNVTAEVKQINKNGDYIKKFNIELTGKANYSERVAKAQGDQAYMPYSTKVLQTKPNLYTPQTDKFDGYAQLPRTLQEPYINNTRKRPKTTIEATKYLLEGKEKNKKYLIGKQLQHITGTIHDYFKAKKEGKIEILKMRTETKMQDFVSGGSVKIINGRELTQPQVLHDPPEKRAQSASRIKCDTIPHTAETRAQTSQQSRVIVPLEEQKLKSMQSYGTLNQELQNAESRALFQSRTYNQTSQSKRSQNVIDQNVQLYNIPHFQERLDIFKKEIQSFTLPQPKEIKQITSKGRFNCHLADNQEHYVQDRLMMKLFQPEQFKSNESPKKQEMEKQRRILAKMKEADMLTRNLKLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QM37</accession>
    <name>A0A8S1QM37_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1870015</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1870016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000196">
        <property type="protein sequence ID" value="CAD8117066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000196">
        <property type="protein sequence ID" value="CAD8117068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QM37"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="104" mass="12542" checksum="FFB776AD71B7EB19" modified="2022-10-12" version="1">MPEPPQNVKEEDKHLDFLKDEEDTDQILIQTVPENQTIFRMPEIEDFLKSKLFNSSQDIRKLRIAQGKKQNWNYPLISFLIYWNKQKKFRQIKSKFSKKTSVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQC1</accession>
    <name>A0A8S1QQC1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1850017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000194">
        <property type="protein sequence ID" value="CAD8116975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQC1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="168" mass="19857" checksum="AFBA25B4BF0B3A46" modified="2022-10-12" version="1">MSSALSLAGQKWKEFWGISKTQNIQLEDKKKQINEADQYIRLAGRAIQGIVFQHQQMQLLYGLLSQVLKKLDELEKSQEGLLLAKTFDSLSSLHSSKIESIHKANDSFQEWFDTIEQLRQMLDKYQENRLVYDHYRLKVDQLKNSKDQQTKVLFNYVQQFSHFQQKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QTU2</accession>
    <name>A0A8S1QTU2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2530007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000262">
        <property type="protein sequence ID" value="CAD8118622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QTU2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8118622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="267" mass="32157" checksum="2F7139FF362A321C" modified="2022-10-12" version="1">MCIHFNDRSTQSRIWLQLGKYLTFDCELCRKSKRLEKLQFCGKFSVLYASVEEFNFHLQSKLIRLEEQSNYIQCEKCKQKFHLNKKGNNIFVSTFICIHQKRIINQCKYCRKLIQKKQYQRCKKCQLLLFFFDVSRQVKCPNCNIVYCESCKEEYQLFSQKFCRCKLVKSSRNLILFQFLFFLFIGLIILVFIGSYYLENSEFYQSIVCTIKDGYSNINCTLDQFYIDTLKQFLLFIILIFIITLMSILFENIPYILKLQHRIQSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN29</accession>
    <name>A0A8S1JN29_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0050507</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JN29"/>
    <dbReference type="OMA" id="HKSITGG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021911">
        <property type="entry name" value="ATAD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23075:SF12">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23075">
        <property type="entry name" value="PUTATIVE ATP-ASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12037">
        <property type="entry name" value="ATAD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="283"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="29"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8043852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="526" mass="60712" checksum="6DC2AD0C697FD2EA" modified="2022-10-12" version="1">MKNQGVFDPTALERAAIALRELNQSPHAAKALEAMIKTEEAKKADKQALQKQHEISKVKVEGEERRRNMDHQKQVNQQIADYNDKLERERTKDKLKEKELTAQRMREEAEESIRRQENMRRETLTLQMKKQFELEEKKITLQAKLNAENYRKNFDLIIQEQEKKSQIEKQAKIELHNLYFAKFQEGFKYLQQNPQGLFTIAKVMLFVSGAFFFSKYSLGLGFKRLEAMLTKPTLVRETSRRSLKWMMPSSKRIFDKIILNPELELTLKLITSGFIAKQSQSAPLRNLLFHGQPGTGKTLFAKLLAYNSGLHFAIISGGDIEKLGEQAVPEIDKLFSWCQSTPKGTLIFIDEAEAIFYKRSSSKQTSAALSTFLAQTSAASKKYSLILATNLPNKLDEAILDRIDQIVKFDYLNEEQRVKLLKKGFEDTFQKSNMLSLILNPAKAFSKRFKVNFNLSEDEIQSLAKQMEDFSPRQIDKFIISLYDAALGQCIIDRQNQYCVDIGFANNILQRSLYENSLRQQWSAQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP90</accession>
    <name>A0A8S1JP90_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0050130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="IVQKGFE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000009">
        <property type="entry name" value="Probable E3 ubiquitin-protein ligase HECTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000002">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF2">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="6">
        <location>
            <begin position="992"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="1293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8043477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1325" mass="159127" checksum="A122A80F62E3DD05" modified="2022-10-12" version="1">MISKFNKQSFLQQKKEEKQSRELERKKIEMASKIFIFYKNTKICNDLKQSIIQQYLPKIQTALSVQFMKDLQTFYKVCNITLNKCPEFLKPFFLLNKKNVLNNETYVLNMLQLFSGCLQIQQNNFVINLFKGNQFLINRLHFSKAILLGFYLIQTSKQASNFIKSMNEILISIHQLLNANQIDLFYGQFQRLETQILQYNLPQLIVQKGFEIFIFYYLNLKRNEQEVWDLGFNYVMLIEQNNSFDFCELMNHIKKSKQYNQLISIVFTSEQEIQYIRKLFNKLQTLKILNSEQLFEILTQLLQVLEQNYYKMNLLSDFKNLQYLNNSEHKKLKILRKKKEFIQFIDQLNFQKLLSLLENQQLTDQSQISKFIYYLINIGQNRKLEIIQQMQKLRYIDNYLTKYLLYLSNLKDFERIPPESISVYCYVKAYEFQLLDDKEFQNIALKNEIVKKEFIEIFGFIIQYTKICIQNDINQHIYKELKLFIREIFDRNQILNFYEWKYEKMKINNHLPIFSLFSQRIQKFNQYLLEDKNQFNRNILALITYDEFGEPIVKQNMKIVIRRGQEFQDAFDXKQASNFIKSMNEILISIHQLLNANQIDLFYGQFQRLETQILQYNLPQLIVQKGFEIFIFYYLNLKRNEQEVWDLGFNYVMLIEQNNSFDFCELMNHIKKSKQYNQLISIVFTSEQEIQYIRKLFNKLQTLKILNSEQLFEILTQLLQVLEQNYYKMNLLSDFKNLQYLNNSEHKKLKILRKKKEFIQFIDQLNFQKLLSLLENQQLTDQSQISKFIYYLINIGQNRKLEIIQQMQKLRYIDNYLTKYLLYLSNLKDFERIPPESISVYCYVKAYEFQLLDDKEFQNIALKNEIVKKEFIEIFGFIIQYTKICIQNDINQHIYKELKLFIREIFDRNQILNFYEWKYEKMKINNHLPIFSLFSQRIQKFNQYLLEDKNQFNRNILALITYDEFGEPIVKQNMKIVIRRGQEFQDAFDQLEKKQLKNMFHVQFVNQMGIPEDGIDAGGVTKEFITRVTNQALDPAFGLFIETPEKTLIPNPAFMKEEKQKYAFIGKIIGKAIYEGVLIEKVLNQVFLNQVLGITNTINDFRFYDSEQYHRICNLKNQDVTDFGLTFSITQEFYGQIIEFDLIPNGRQINVTNDNKIQYINLYCHFRLNKQIKEQSQIFREGLEQVIDPEQFKLFTNQELQLLISGQPVIDIQDLISNTKYQGYLPQDKTIQDLWSVVNDFEYEQQSQFLFFFSSCSRAPTQGFKSLEPPFMIQKVPIQHPNEIQKLPTASTCFNILKLPDYKNKDILREKLVQAISSNTGFELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPD1</accession>
    <name>A0A8S1JPD1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0060417</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPD1"/>
    <dbReference type="OMA" id="GHIYKHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006862">
        <property type="term" value="P:nucleotide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="240"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8044333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="38222" checksum="21DB76206CD22B70" modified="2022-10-12" version="1">MSKDFSEYLKGIKKEETHDDIDLKELESVFDEKKYEVKWWQIAIGNSFTTTALGILTQPLQVIHTTFQLSIKRMDFQVNNLKDIMEAKITLGEQKKLEQILKYKIINYQGASYQNYNAVFKGIGEQGFLGFFKGLGTGIAFQITNSQLRYFLIQKYFNEKKLIQNLYASLVGMVVDVFTNPLMLIQSKMIYQNRLPNFRTYRSIYDLLKKERNLYKGALGHIYKHIFISLSQMPSLYINDTRLCLGYLLMIQQIVSYPFLTVIRRLHCQGSSPFMLQTNYKGFWDGAKQIYQQEGILTFYRGFLAFGIANSFMAFVLYSTQLLGESQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQM2</accession>
    <name>A0A8S1JQM2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA ligase family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0050544</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQM2"/>
    <dbReference type="OMA" id="YVPHKIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07896">
        <property type="entry name" value="Adenylation_kDNA_ligase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08041">
        <property type="entry name" value="OBF_kDNA_ligase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029319">
        <property type="entry name" value="DNA_ligase_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050326">
        <property type="entry name" value="NAD_dep_DNA_ligaseB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006592">
        <property type="entry name" value="PRK09125.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47810">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47810:SF1">
        <property type="entry name" value="DNA LIGASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14743">
        <property type="entry name" value="DNA_ligase_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="5">
        <location>
            <begin position="282"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8043889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="443" mass="51261" checksum="49AF2E46311D23FC" modified="2022-10-12" version="1">MNSEIYLVKQNQLFWHSKLLSTNPVTLQIRSGILNTNWEKPDNLQIELKKYNTLQEGILDIGEMLKLKLRQGYHEIKPSEIVKTYEQISFPEAEYKYELDLKYKQFQQQKGEIDDKQLTKKEQIVQKIEKKQKLRQPGLGLPDKSCSELLDDATQGRLISVWPKYKMRPNLGENLGLGPTFSKSQRISCLLASNWTVSIDPKGYYMSEKLDGMRMIWTGMEMFTRNGYSLKFPSYFIEGWPTTYLDGELWINRGTFNKVLTSVRKRTADPNAWKIIKYMVFDAPFLQEPFSDRYAKLKKAIEKIKNPHLVYVPHKICKGKEHLAEELAEVQNNGGEGLMLRDPDSLYDGTRSKSLLKVKSSLDAEAVIIDYIEDTHMKKDDVLGGFLVRNEEGLEFEVGLGLKIGIKKQRPPIGTKITYKYCGLAENGYPKFPEFVRQYPQDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQS8</accession>
    <name>A0A8S1JQS8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH araC/xylS-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0060041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8043959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="109"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="348" mass="40275" checksum="76C5E8077DB87980" modified="2022-10-12" version="1">MSDPTESQQKQNNDVIYCKHHPEEQVTRVCNNPECNKDGFICSKCVHEGAHEGHPSFGYAKLKAALIKFVNSSRTQQDLTKEGQLSYARMQNYLEETSQSIYKQAQKLNKLSQFMENLNETLLSLQEIGEEVDIAYTILYNMDKKESGVDVSTLNDYLNQFRLLMDINLDSQNHEVSIKPSSISQLRIKNILDKKADFTSLLRKTVEEHEQHFQKFLKEMGDLLKENKQFLVPKANETEENRNAEFGKRALYNSYYKQDTEKTEKSKKKVKGQVKGKVKHEDDEEMSMGREYQIGAVEKCGIPKLDQGQPPQGIKKQXNKLSIKKKFDFCYKSSLINLGWFTKYSKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRG9</accession>
    <name>A0A8S1JRG9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRG9"/>
    <dbReference type="OMA" id="VMINELM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="432"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="556" mass="64734" checksum="9F05BE2C8C1EA91F" modified="2022-10-12" version="1">MFEDRFLETIKQQLLISKVVPVTINPKNILKSLLAVENKLEEENLYQSICKKQELIIKNLNEENADVILSICKFIKLEIIKQMEGSKEEIKQGVLKQIEIECINWPQYKNPRWPEYLEYFNNLSIQLINHTYMLMEQILIDLTTKPFVCEEFHQYITDKLEHCMIVLSMEMSDFARVINNEIFYWELKKYFNSIIQNVHLYINRTSLFCNTQMEYLNETLTKYHTLANIRTYQILQIETSQYIIDQCHVSLKEMLFHRIEFEIFDKDLVKQIIRHFYEYSPLNQGLYEMSTLTKSFQDAIQMYYILDDKYFATADLDSKLFAQDEECIKYKIWLNEKRKKQSALANQPVLKGSVGAGLGCLIVDSVDPKIPWDEKLKRAGISSVATGLVGAAMRIPVVGVILSQALLFYAIRVQANNKVVDKSEKMKNIAHLGFQSSFGIGTAVAGQILIPIPVLGALIGGTVGGVFMGLYNKFIIPKTKPSIKVMINELMERQYADGLFQYDDKVLKIMKINQQHYFGLKPENLNDCQWLTILMDQRRSMNYNNKLKRNLKMKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRQ0</accession>
    <name>A0A8S1JRQ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRQ0"/>
    <dbReference type="OMA" id="LIVKFEP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="933" mass="111884" checksum="14C2DC2CE8F42C4C" modified="2022-10-12" version="1">MDLNDQQKGLVIQNQYFLPIKFKNQEQNIKVTSEIENLYKYFLKVKIKQFKPKIIILGLEIDNSFEIDSYYLQKIIYQLNKISQNQLIILPIRKSTNQEQYWQTLVQCINAINTYNKTKKNHIQDNNRMRAQNQLAYIEYMSKTNKEKEFYKKQFKSLNLLQKFEESTEKKQNNQEIFKFPIINSKNVIEYVILENSENGQQIYIDSETKYYVSYFQENTENKLTISVIKNQSLYLGEINLQEIEIKNVNQKKIILEEIVIFQDNPNSSFLLDQQKFYQITLDINKMNQFVGTVIIYNFQQNQNKIDKFSVQNNTEIQGHIKGFTIIKHSDTSFSLLSGCMDGELYQQIGIKLDLVEQNSLKINQYQKQNNKLPKGYDYPLIVKFEPNNQQTNNSNKYLYFESQTKYQVQPFSPEIHQIIFSYNQQNITCKSEILTNITNIQLFKKYSKLFRSDKINLKYSKCTSNIIVIDLLVFEKSFERNYLKDFNDNNKDIVLSKQPLQNYLHFQCCYQIKSFQTQLKFNLNINSILLKTNNETLNQRCLYNNWLEDQNFQHIFLFNTSQHIYLLYYLKDKSGQYYKKKFTFYCDENPKDFIPYDEIMINDFYNNVIWIVRQYYDRNYPYLVIYNCHWMRLQGIKDDDVNILVKVKIDIIFELNINSQQFNLIGLEVRKTDNMLLICSSTFICQIPIQKIRNYICNQKQIQKQMIEENSLYQIFYTQLPICHNSQTILMNLNDTTIAFFYQYCTINCLDEQQGFYQIEIKAQIWSQKKQLDQNIENCLILSNSNDNMNYYNQDEIQDDKALIKQSFNYEAENTLILAKLISIEFKGQFFQYLIFFEQEFSQFQVIEYYFTSKCNSIIKKRIIDLQQSQGTIQFLKSMSTNKYETLILYENSTQLIQSECTQDIFLNEETEQDFGQQKVHNYFDKQEYLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JS70</accession>
    <name>A0A8S1JS70_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JS70"/>
    <dbReference type="OMA" id="IQWINCW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035744940" description="Insulin-like growth factor binding protein, N-terminal" evidence="1">
        <location>
            <begin position="16"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="587" mass="69391" checksum="4E17433D9ECDF482" modified="2022-10-12" version="1">MLLIFLFFLIQWINCWNVITSKKIIEPINNDNFIMEDDYLIVSNGSYLLKYEGLQGYRKLHMIFDAKANLSQVEILLKVNGIKRNFLVVEEDFKEFHIEFEHQAPDIKIQFNFDEQIQFSIRNFNLFIEECPKNCKYCLSGYCDLEILDQCEKLRYKNQCVDECPKGYISEFRECIKTSLNQLGLNQMSIDNDIGQTKKFGENQELHVDFGQNFVGWMEIVFNCYGKQQEHYNSILKIFKENAQPELIHPFLPIYYNTQSYKQCKQRFELDCIQVKGYLKMEFQEQNTITIYSYSKNVENTGFWNIDEIKFYDKEIKYYECTINECESCSFNQVCSQCKVNLYLYENQCVKQCPYFTIKKGGKCIKIDEEQKDVSFIIKLDSPFQELLSIFGQKTFDMKEQISFRYENGTTYIGGGLKDNWRRTTFTKQLNLGKHYSIRLMFNLSIENSTSELDCFTYTIDGKTYLVHKNESYIDHPLYHNKNILNLNLGCKISNNGRCVISNYYFMIMKCSPLCLQCVGPLQSDCKAYQKDIDKFDYQKQKCLEGYYLDDYGCIKCSVGCLICETYNKCLKCKDDKDGEFYCLPYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZS1</accession>
    <name>A0A8S1JZS1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120385</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZS1"/>
    <dbReference type="OMA" id="DIFREMF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="227" mass="26677" checksum="B71F5C9EA9FA2659" modified="2022-10-12" version="1">MDADIFREMFPQDYIKRFLEKNVRIDGRQFKQPRQIIIEFGEGFTNYQYADCFISIKHIIREIESKEVDLNQCIRLSIIMDNQEDLSNIDKVRIEKHFLSQIQDVVIQIVDPQRLRTLNDKVKQIELYIRVAGGDGSLLSHLINCVSSSLQQIKHFETQNSIIYKEIKCQTFAVFEDQILVDPSDFEEKNSSIITVIHILGDEQFILKKYDGKSMNYNLIQQIIQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K298</accession>
    <name>A0A8S1K298_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0130088</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K298"/>
    <dbReference type="OMA" id="NRPHNDL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07852">
        <property type="entry name" value="STKc_MAPK15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000238">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001058">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="19"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="499"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="562"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="507"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="572"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8048808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="655" mass="76412" checksum="6AC3A1225EEFE505" modified="2022-10-12" version="1">MSNQNDQHNQIEEHILKLYDIQDFKGKGAYGIVWKAIDKQTKQQVALKKIFDAFQNQTDAQRTFREVCFLQQLTDHDNIVKLLKVIQAENQKDLYMVFEYMETDLHKVIRAGLLSSLHMQYIIYQLLKCLKFIHSGELIHRDLKPSNLLIDSDCKVKVADFGLARSVAQSENQYEMPIMTEYVATRWYRAPEILLGSHNYSKSVDMWSVGCILGEMILGKAIFAGASTLNQIEKIIELIGRPKQDDLELINASLAQQVIDGIQMQKRKSFAQFFHNASSDCIDFIRQCLNWNPQKRMTIDEAMNHQLMKEFVNTEDEKTLKSIIKIPFNENKKLTIKDYRDKITLNYEQILKEIEQNTFNKNLNKQYLSTPQNISSYLNNFDSKYKQNPNYQTSQPVDKPKKLDSFYKPEAINPYSTQKKSDYSPTMKPTMSTNASNISPKFIQQKYNTTQNQSYNQGLRKTYTNIVNRPHNDLNKSPEIKKRTENIITNVTKSYSFHGEPYEKRVPNSSFSTQNKNKPSTSVSYVRPVQIQQLSNYNTAQNFSQQKEMLRPKDPIMLNQDQRSKSFEKSPININPTSPYQRQKTEISKPKFIPQSNINNSVAQVQKLLQKCCQVQNYQQHKKTLSVIDPTKQQPQQIQQTINYNYFDIRNSKFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2J2</accession>
    <name>A0A8S1K2J2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0110254</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2J2"/>
    <dbReference type="OMA" id="HAYIFME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="6"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="357"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="512"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="398"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8047090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="531" mass="62826" checksum="1B91D9B714213E30" modified="2022-10-12" version="1">MKDKYIVEEKVLGQGQFGSVQTAKSLENDQLRVCVKIMQKKKDYGQNVLQTVQEEEFNICKKMVNKKFDNLVQIYDVYDQDYNDTQAYIFMERCDKSLKELMNLKKSGQEKKFTKEELIDIINQIAKGYNQMISQEIIHRDLKPENILCNIVDNQIQVKIADFGLSKIVDSSSQKKMTKKVGTMYYSAPEILNEKNPDDYDNLCDIFSLGVIIFELALLERQFNYKKIEKLKEITFFQLHEDVIKNKEIDKDILELLDKMIVYDPKLRLTWQELLNYFDQKQKQSSLQSDLQQQQLKRQQSQQNQLFQNPQQQQGQFQGPLNINVQKQQNGLNQQTQQQLFPNPGFQQKNFQQNQFPQQGLPQFPQGNQGQAQNPNRFSTISKGQQLPEQSSQAPNVQTQAQPTQQQQQTKFQFPQTINNLNQQNNFQQGGGQLNQQQKFTQQQQFTQQQQFTQQQQFIQKQQFNQQQQFTQQQQFTQQQQFTQQQQYTPLQQFNPQQQFNLQQQFNQQQQFNQQQPQGYPNRLMTMASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K390</accession>
    <name>A0A8S1K390_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TFIIS central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0120353</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K390"/>
    <dbReference type="OMA" id="QTNRRAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS central" evidence="3">
        <location>
            <begin position="17"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="445"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="771"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="876"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="929"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1004"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="555"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="827"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="204"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="352"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="448"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="476"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="771"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="788"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8048175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1033" mass="121861" checksum="E0C98A76A93914D5" modified="2022-10-12" version="1">MFQEVRPVENTNYDRRVRENQKNEFKKILEEDQISSQIAEQLATSLEQEAYTLSGGDHTRDYRTRVNTVKMRLKGTKGQLIRTALVEQILTPKELMSLDMSKLSEDSVQAFLQKEQRGLPQPQTNIKKNVPQRGPPTRPPVAGIPPIKNQVVVPPIPQSTTQNSSQIINQQQISDQTNSDNQNIHTQAEPQHINKEQEINLQTQPKDQNPQNQKQVPKQQIPPPNKSKQVNSTQISNNQTEEYGIKQQEVQQQNKNLNTQINNQLSSDITTKQEKTEVQKNDNIPPPLSQQQLTNQINFQQVQNENVEEEDLTNFDFLKQNQEKLNQLAEQLRSEEESEESQESNIENQENYNPIENANFSQTDNQEDNPILQFQNTITKQNNQDSSENIHHTEPEQQINNELDKQQNDNFQQETIKQQIHNSQELKDQEFNEQQISSQIQSIENQDEVPHQKLKEEETQVQQNNQDQQIKHQKLNSSHNQQEQTQQIISSQTDTQNQRNEQIVKQQNELIESLNLTSQTPKFNIQLQQQSTQQIDVAVFEQMIKQRDIYYEKQISQLIKQNKDLQHSINQFQQDHIKTIQDLQTKNVNYQTQIQQLQNENKLLKTSLQNQEQNQLKQLNQLKEQILSERKQFQKLQEMQQMEKQNEKHFIEKLHSNTQQRKDIIHKYRIQSTQSNLDDQITILQGLLSKLQPPQLPRVQQFQQPTQIQANTQQQVVQTLVVEAPATHQHHVQQQNQQYWANEDDVNLQGQIVNADSGSTQLKTANNQIQVQDVQDAQDEQQQFEQQDQQDQEVQQDEEEENQQFDNITNQHAIQEQQQAIHNQPHFKQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYQYHQEQQQQQQLLLQNSSQQHQEEQIQNNEISNSMDKKVIHERQQNEHIDQQQANSNIIKNEIQENTYHPPPPKKQNHQKQENHATFAHPRLAHKQENNHNQQQNQQQNFQTNNPFFDYQGAEEYFETSNQTNFQQFFEKQIEIPKQPTSQQKAKQTNRRANVPGSLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K579</accession>
    <name>A0A8S1K579_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130176</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K579"/>
    <dbReference type="OMA" id="DPCTAIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="631" mass="74408" checksum="FDD70723ED7A2855" modified="2022-10-12" version="1">MYNDKTIYIVEQLIQDANSDLVTLLQVETFMKYVRVSDAIAIEFIIKNINQLLRILFINYDQIHRSQYIIYSDLISIVWEIIDLNIEQILQQIENNSTQVYDLLNYQNVPDVQWAQAHKFLQFIKQRSWCLIPKALFEIDYLLIKFLPHLHNKSVALIILLFFENQYHDQQLSFLKKGFNQFNEFDPCTAINFTFLIHEIITRIATKELINFLLSSQAIAKSFEVLENCNLSFIVRKNAAHILSLISNYYSLDMQNLYLDEPSNETIIDNFRQTEFYQTFNIQIIKDILNNVLLNNCRLGLLAVKLIEIVDNLVRISDLELWNKIDKSNIMEIILNLVKKFKNSDIFISYVNNMIIFILDRAISDFHPFWCVQIFTKYKLYAQQIMQFNRQILIFEQQLQVKLIRDSDFLPYYNELMQIENILKRSQFWRQSKEQLQKYEEKHIYRLGEDSDTPSIDYPLIVSAIDDECIDEIIEPKDNIFIDCLNRQSPIDSLNNNDDSDDDDKLIGAFSGLDTLNKLKNVFHKMDLKDQEENQQVQENKQQQQGKRIRNLSNSLNNQIQQGQRDIISRSLNSDHDSLQKLSLSSSTNLKLMTKTFDSFTIEILGNTKVVKNNQILKVDEIDNTNNIQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7B8</accession>
    <name>A0A8S1K7B8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">VTT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0140118</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TMEM41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7B8"/>
    <dbReference type="OMA" id="CIKIPRD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045014">
        <property type="entry name" value="TM41A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43220:SF18">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 41B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="7">
        <location>
            <begin position="97"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8049695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="266" mass="30383" checksum="501418694CDA4DF2" modified="2022-10-12" version="1">MSTEIDINKLKAEIKNDKQRRLILFGIFATGLIGLYVLVHFSPHLTQEERKIVYRIPKYPHHIAELLNVINRYAENNQFYVLFAFIYLYVFMQSFAIPGPVFLSLLSGQLFGPIPAFLLVCLCATTGASLCYGLSYSLARGLVLNRFPNQIVNFNKKIHQNRDNLFFYMLFLRFTPLIPNVSINVSGPIVGLPFKYFFFGTLFGLMPGNIIHIRTGLLIQELNDFSTSYQAILTLVGLSLIALLPTFFKKKLQELDEKGLNKSKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9H9</accession>
    <name>A0A8S1K9H9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9H9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="23699" checksum="CBFB740DBC1552D2" modified="2022-10-12" version="1">MIQTFFGLCHLFNRRKLKKNLMIKYQYFVETLIVLILIQYNTQKQQNLLLKELKCVQIKSSYKMRYQTLWSFQTKLTESIDQFHKFTNYTQNVNYYIKYCLNDALITFTNIMQKQKRFQEFQKKAYYNKKQLYLILNFQGIICLFQHILILSIQSSQHTHFFVQIIVSRISNSYSIVYLTVDLTFLASSLQKVNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9W9</accession>
    <name>A0A8S1K9W9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0170066</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9W9"/>
    <dbReference type="OMA" id="PKITSNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8050455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="433" mass="51734" checksum="BE43EB5F045729E0" modified="2022-10-12" version="1">MNNQKESENDDSDQSQVSITVQQKNKERQLRKIQKLQSLINKPFQQLTDEELNKRRILSKVSDLIGKTNNPHQFLQPLNHKFVLNINHLQNASIPKITSNQTDPEKDYVDYLMMKKQKQQEQMRRQAIKKQNLKENYDQLWQQEEDLSDKEIKDTYNVNKALTIVHKSQVQKEKQLKSDERIYFGKNSQSVQRNSNFQSLTNSRLILMQDQDINETECREQQARRTLKKMKTKQVINIVQQATKKIFNKKDEQIQKELIMHQVYKQMNKEKKKESIKSIDSIFRNSQNEEIFQQSQNQKMITNKILSENKFFQPKKLNEIKPIQSRVQLSHIIQKQDSRTDDSPPRFYSRKISLINDKITNLVSQIDLIQQKEIDQLTFIKQLENEQDLYKTYRESLQPDQIQDTLKSLLGPSNNNYRKPFRFNNKKFFNQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCL5</accession>
    <name>A0A8S1KCL5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0180080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCL5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8051405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="160" mass="18286" checksum="EDF0E0A102E9F8AD" modified="2022-10-12" version="1">MLYQQLNKNFKKALSAFQITSLLPDRTMSSTKNRVYKKSQTANSSKCLFVPKKKEREQIYITPPSKHKNPLSNIEELNIDSNCSTDSSQRLENLSPVYYCPKGKEPCGELQVVLDGDVIQVISQTRNRRKSRKFSDQETYAMSNFIAGPKCLDIPCPQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KD11</accession>
    <name>A0A8S1KD11_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0180193</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KD11"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8051575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="120" mass="14180" checksum="4BFD9ECE17A61998" modified="2022-10-12" version="1">MFVLQPLVKKHSIKILNQEDINQYTMEKNLLNMNLCQKGFSLDFNLKIHISTHTGEKPYTFKYLDCQKKFSQLSNLFAHLKNHQNSDYADNVYNRLYIVSDSEEVIDEFEEEIGNEDVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KG42</accession>
    <name>A0A8S1KG42_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200211</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KG42"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="145" mass="17393" checksum="6F49751D441EF1D9" modified="2022-10-12" version="1">MNYPKRFSIGKMYQREKGIYLKDNNCISQSQDYQTKNQIDYIKQGVKLQDVQLATFYLKNGLPYRGFKATDDIPINCLLVQVPRQLIISSQTAFESSQKAFYYKHRDFFIDHEEKEEHTIIAFLIMNKRKGFKSKYYRFIKQLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KN76</accession>
    <name>A0A8S1KN76_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0250020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KN76"/>
    <dbReference type="OMA" id="YPRRNID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1012"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1015"/>
            <end position="1025"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1066" mass="126294" checksum="5516B044E6D67EF6" modified="2022-10-12" version="1">MILEEALGVEDDYVRLQKIAEGIKQISQSLKTQKNFNSPSLIKQLICSYPFSDTTIEMLLNECFEQHKSDPKFIMAFHKALQEVAILQEFRQFLGSRSLFNKQNRQLSHNNNITSILKEQLKQGSIPFSREVIERLIIAKRFSAKFIIPIFTLSKEYKFDLEQMASIFKLFLMHTKNEEFQLQIIDIYEELKLSIFKDLEWIEEHNQELEDYFIQNKIKVPQRLIQSKLMHIKNKNKKLLHQHFIKKCNMKSETESDSLSVSPEQPKIIDYKTKIQQWLCIKETPTIDLDNYVYFTNSENLNSLLSFILNPCKIEIIADWELTFKNVETKFNKKHFKNIPIKLDTFQIIRSYKTVLIFQNQNNIRQIDKLLTTFIEISFRKIYDSLISDEINLNLNHLVNFIEQLILLNPQKSAYQILEYNLLFTFSLYIYNHQMTTFIGDMLSLWIDKFKFGFYLQEQIWKYLDYTNWFNFFFQILIKQPQRNEQCYQEYKSLKIDGILAFVKLLKSPEKTQQTQNDFQNSVTSRQEPLTPIEFSEEQQILNSSQWILDNNSTANQIMEDMSQKDIDNLKHFSIEKNKSIMTELQESSPLRIRIMQSSYNKGFYGQSSDITNKTLLSPKGKMHHSSVSLPSIDNKVHKSESVDSQNCQNYASRLRNKTYIISQDGFSTTKLISIYPRRNIDLQFTHFETAQSFFTYNKHTLKGCTRVFKILNDSIFNSLYYQENLIKDHFQKQQFLNLAQRSFINERTIITLFQIYLYGIFDEESKILQKQSIECGLIINEIYKNSLILQEKQLNTLLKNCFSQVADFVSKKLIELNNYTQIPEFKFKQYLLFSTLVNGLHYFDLYDPNRDERNIYKYLNETVMHILIIWFFNSHQNNIFQLNFVKLIAILFSRAPQYQLQNLLFHVGLLTSLFNGYTTYNKNGCKNIDYVESLYMNIMYLIYIINANVYQRQLTLLISNLESSASWKGLQQCLCEVQTNFLNQLNQLMKLDRSYSLKKIKSSQKIILQPNSKESVRGKNESKNDNSQLNELLKQKSITKESSPVKQQMQIKKLIRYRTKNSIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRA4</accession>
    <name>A0A8S1KRA4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyrrolidone-carboxylate peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0250148</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRA4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.20:FF:000003">
        <property type="entry name" value="Pyrrolidone-carboxylate peptidase isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PROTEASE FAMILY C15 PYROGLUTAMYL-PEPTIDASE I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402:SF1">
        <property type="entry name" value="PYROGLUTAMYL-PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01470">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8056971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="23907" checksum="FD40F1F472BABF8C" modified="2022-10-12" version="1">MQNKNYKNINLSGFGEWGKTERNPSALLIQQLSPQQQHDLNIQSIKILKVTKEACDDYLSQINNKNAINIHLGLFDELQNFQLEMCAYNKLDYQLKDFNGCKIRRAKIDQEIDMDEQIKSSLNISQIIFQLQQLGYQVNKSYDPGRFLCNYLYYQSLKQNPKTIFLHVPLYETIKEEKQLQFLIDLIQTLHNTNNEQDIIKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVS3</accession>
    <name>A0A8S1KVS3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0270252</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVS3"/>
    <dbReference type="OMA" id="PKVQQHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035754878" evidence="2">
        <location>
            <begin position="25"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="49"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8058501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="138" mass="16118" checksum="A28741C8155F99F7" modified="2022-10-12" version="1">MEILHLALLIVISIICFKIGQGVGQKEVEKQQSQYIQLQENEQLIEEIDEDFIKQVEQQEQDLKEAIKKFNEINQRDKQLQKQLNDLKNAQTNNAKPKVQQHELLSDKDFDNLLDEGFVVDQEINETQLDQDLIEASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWK9</accession>
    <name>A0A8S1KWK9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0280275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWK9"/>
    <dbReference type="OMA" id="QKNEEQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="1">
        <location>
            <begin position="94"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="30657" checksum="BD76ED234ACE6DBB" modified="2022-10-12" version="1">MENINIIISSLSGEKLKRYVPVQTKIIDIKEFIAQKKGVPIQAISLKFNAKDLDDQKTIQECNIKNGNSIILMIKKILNQNFGNVQDVQTQLIQKNEEQYKELIDIGYSENDIIDALKKTNNNYQEALILLSNNNIKNIEEEIQFKIPTQKEQYQALLSEQIVSELRQRVSNQTEWNNTIETIKQQNVQLSILIQQNQDQFRHLQQQHVIENIINIDEEDLKMLTQLRGFKGNLDDKSILKIYLKNERDLFSTMQEIENLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWY2</accession>
    <name>A0A8S1KWY2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0280325</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWY2"/>
    <dbReference type="OMA" id="GYERNEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000537">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="56"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="312"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8059287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="371" mass="42904" checksum="0887D6FE9C3D5969" modified="2022-10-12" version="1">MGMCQNKQAEYNSKIPDIASEILDEDEVDEKIGETTQIPIEGENGNLKTKLTLSDFELLKVIGRGSFGKVLLVKRIADQQLYAVKILRKKLLQKKKQQEQAIQERKIMTLVNSPFSVKLHFAFQTPSRLYMVMDFMIGGELFLHLRKRQKFSEEWTQFYAAELLIALDNLHQQKIIYRDLKPENILLDKDGHVVLTDFGLSKLGYERNEMTYSFCGTPEYVAPEILYQKGHSFVVDFYSYGALIYEMLSGAPPFYSKNKREMLKNRCEKPLEMKPNFSLQAQSLLKGLLIKDPGFRLGSNGIQEIKKHNFFSGIDWQLVEQRKLQPPIVPRIQHLEDLSNFAPAFLQQPLIETPDSQQNVYFEGFTYQQGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0W5</accession>
    <name>A0A8S1L0W5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0300172</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L0W5"/>
    <dbReference type="OMA" id="ENHEITH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000023">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035856843" description="peptidylprolyl isomerase" evidence="4">
        <location>
            <begin position="16"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="5">
        <location>
            <begin position="27"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8060511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="190" mass="20569" checksum="A669CD92BE0C789A" modified="2022-10-12" version="1">MKIIILCLLSFSVLSQLSKQTVTHKVKIGVTIDGKNEGEITLGLFGKVVPKTVENFRALCTGEKPGKSYAGSPFHRIIPNFMIQGGDFTNGNGTGGESIYGNKFNDENFNLKHEIGCISMANAGPNTNGSQFFITTADTHWLNGKHVVFGRVIENMELVKKIEAQGSQSGQPKTKVVFATCSAEVVTDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1D3</accession>
    <name>A0A8S1L1D3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1D3"/>
    <dbReference type="OMA" id="HYLLKCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40535">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40535:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="293" mass="34128" checksum="DC3A5F05C0DFAE47" modified="2022-10-12" version="1">MDLMKVKMMMNNQLLNLNKRYKQKQEIVMKIVMISQIIQLNKEINKNEHKISYSKIDNIINILLMQILHLFLIVAVLSCDIDEAAKAFKSKQIRDPIFPYTKNPYDIVDPNYLQKVSDNLQDTTSVCAIKYDDYEKQIYHLKHFNSKEEAEQNQFIVTHQGKCGACSTLQDLAVYLTNDLTRPVRKCGLMYGLSQHHLLKCIKGLGFTDTCAQVWLYNTLNTKKSCFWPCIVSFMTNEDFVKNGKLNKCLQCDEDISGPIFKYESGRTRRNSGIKSEIDRPDDQIYDITHCYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2G2</accession>
    <name>A0A8S1L2G2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0300093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2G2"/>
    <dbReference type="OMA" id="CGNTAID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035944324" evidence="1">
        <location>
            <begin position="16"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="178" mass="19434" checksum="3E02DAAFB246C940" modified="2022-10-12" version="1">MKAILLLVLLATGLATESFLSTSQDIKEMELSESNFQNFLIGYALGIGLLDAIPDALGCVGTVTGFKDLFQKAIDDLKTNDLEHIVEGVQIFAKLFDGVASDCGDTAIEAVTSYKNIMAIIRAKGFIKQGWINIGKNLETVVDDIQQFFFLGDDDWMLKGAYIGDIVKIYVTGIPNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4R3</accession>
    <name>A0A8S1L4R3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">50S ribosomal protein L20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0300198</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4R3"/>
    <dbReference type="OMA" id="VITHQQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07026">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.20:FF:000001">
        <property type="entry name" value="50S ribosomal protein L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005813">
        <property type="entry name" value="Ribosomal_bL20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01032">
        <property type="entry name" value="rplT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10986">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00453">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8060563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="19698" checksum="4099292ADD4EAF72" modified="2022-10-12" version="1">MANWSLKKYLELAKGFQGRARNTSKIMMIRVQKSLQYAYVSRRLRPRILRREWIQAINAGVREHRISYSQFIFGLNHSNIQLDRKILANLAINEPFSFKAVVDEVKIQNSMKEKPSDQVSLDQAYEKGLIANNVTQTWFKRGIKEGHKLYGLKRELTPEELKKFEKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5E3</accession>
    <name>A0A8S1L5E3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0290288</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5E3"/>
    <dbReference type="OMA" id="LRFYVEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019">
        <property type="entry name" value="KDA ANTIGEN P200, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="232"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="253"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="499" mass="60156" checksum="1CEC04D2385C080A" modified="2022-10-12" version="1">MQNRQELQSNPIFQQVCQKLNFQPDQLEPQDFEEFYQANQKKYNMDEEAWVVLWEGQEEKKQNKLQQVLKIVELEELKAEEEKKKSFAHLLIQLSQSQSTKIQQKQLEKLRAGNLKDLRFYVEQQISQLTNKKSFIPRYKEIDIYKERRMIEMDQERDKLLETKVLKFSPPLETKKKIDSNLVFVGLDKLAPRQIKLSSYEFDVENYDPEDDVNYELEQQLLDQIEEELRQGKRTDKNVTEMVRRPEVKKKKGDQIQEVQKRNQEQMEEKRNQLLEKQEKEEERIIQRQLQKQREQKQKIISNFMRQQDKKENQNRIQLVDELNNELLQKKIAEKNKKINKFQKNKSNYHKQKFEINRQLDDYDMNLNQLLEDYQDGIISANVLEQAISDVPVNIQVQKTQAQKVQLSQKKKSSKPLSQKNQQELIDLEQKHNLELRMLIEREELAELNRQKSINHGVSQEILELFEKQREASKKRIQNTLTKQKMEIQNKLRLYSKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5J9</accession>
    <name>A0A8S1L5J9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0290318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8060026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5J9"/>
    <dbReference type="OMA" id="IMIRRRD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="96" mass="11129" checksum="5FECA38A3DBD6351" modified="2022-10-12" version="1">MGCTQQKQSSPSRKRNDDSLINGQFDTKLTQNVITIQYLNQKQIRSKINSGRHYNFVLQHVILQNNILNSTIMVRRKNQENLPSKKEYMTSSNGCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5L2</accession>
    <name>A0A8S1L5L2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0260298</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5L2"/>
    <dbReference type="OMA" id="CKVEIPC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF4">
        <property type="entry name" value="TONALLI, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="575" mass="68068" checksum="1A7D438A6BAA178A" modified="2022-10-12" version="1">MDQKSPQKSSSPIKMSLNNIDKIIYTIKDSWKIQQDENQKLFDQFLQYCKSQYPVFNIKFTCSISGEWLEQPVSFMQCILDKKHLNDCFDLKQILKYLIQNDSNFMQDGYIQCPLCRSKSHFKKDIPLIPNLFFQQLRQQLNDKSKPITYYLTTQTLIPLYPGFLGKVQFQYNNQIQQIFSQQCDVQQSFSNLRKIIKGNESFKYSHVCLLGNIRIQIPVRGKQCRHLECYDFLYLNWYMQQRDQKQLKCLLMGCQEIVNLNELQLDQELYEIGLKSFNFSSDFIYDPQSQKLLDTYSITNYDPQIVAYKKLVPTTTETMNYIKSIYDLTDRIYSSTKNQELTDKFYEHPLNGEFNIIKDQSTNCKVEIPCRCIRCDKVNVCDLRFMSCILNQVQYPENRNGVKTYSVSCPLCHNPFSPKKRHRDQSVHEQVYVDTKMLNYINTIKNFNYLNKEKYQLFLQKKLYPSLRFEELESPIQELDQFNLKLRCLISNQKLRNPIRGIYCQHPEAFDENSLIELCSTGQLDLNLAQCPQCQQSIHKLTHDNTLKELLGQLQGDFDEIQVFPKQKLIKQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L7L6</accession>
    <name>A0A8S1L7L6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0340323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L7L6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="186" mass="22739" checksum="6B51AC73CC738045" modified="2022-10-12" version="1">MKICSFFLKFYFSKGLQFFNPKKQHQQRKKYIFMLIFKFFFEKLCKKLQFHITMILFFRRLFLSYKFFNSVGSSFPVTFCKLIEFFYFQKFIFSFRTSKSGFEKADNFLFGVQKGDYYQQMQKIVDLLDIRALFKNDAPIQCNLTNFQKWLLDIIIFRTIKILSLFISSIFQLKLKFIYIKDDLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L8Z8</accession>
    <name>A0A8S1L8Z8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0350255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8064017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L8Z8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016286">
        <property type="term" value="F:small conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015449">
        <property type="entry name" value="K_chnl_Ca-activ_SK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10153">
        <property type="entry name" value="SMALL CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="197" mass="23698" checksum="979EA13054154474" modified="2022-10-12" version="1">MWIAEKALQRENLQTIQLFKHCFLEVILSLLAYAYEDYVPVTNLGRIILSYAAYAGFVMASIIISAIIQAFEQEYGEYQACVLLDKLIMKESMYQVTSNIGEQFYYIMKFDKNPFTYNRVKIIQTELKEFQMLKRQYKSIISENIVNLFKRRSRESNDILEEYQDIMKMCKYQNKQIQEMLEELISLQNYHYHNWYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LA53</accession>
    <name>A0A8S1LA53_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0290214</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0290215</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LA53"/>
    <dbReference type="OMA" id="ENNAIYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8059884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="291" mass="34096" checksum="09204FB24E4AADA6" modified="2022-10-12" version="1">MQQTRSPLSPIDSNQNTYRSQASKTQDINYQIQFQISKQKLSTVPDSRQDNNAIYMSPRNTKLKTQTILSPNCRSPLTSRKEIKPIQSPYNLSIHSAQSPIKIGNSTAHNIKQELDYFKQKNNNLQKQILNLTSEIQRGQSNTLIKELQQKIQLLTQMNEKLTQENQELQQIGDKSHLKKNIESQLQLIKENEKKLNEIKEKQQKYEISKLDLAKNSTTDYITQLILDLEERVHILIVENANLNKVFNQRIKLQERFNTLELQLKQAQVQFQKVKIEEKLIKTKYNQIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LB90</accession>
    <name>A0A8S1LB90_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0340351</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1281"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1458"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1585"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1811"/>
            <end position="1829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="774"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="783"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8063475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1869" mass="219520" checksum="2B5E8DDFF4F3E89A" modified="2022-10-12" version="1">MKWEYFKQYQGLIIQTNLSPTINNFSYAKFQIAVYFLQLSQFLIDDSKEQVFSLIKSFTKICLICPYFMNNKDINILIGIVITVLNCLPYIIITYRFIMKSHNSVDSISKSVLDLTTIIINNYFLNFTWLFYVSQLYYICWNLLNVSDPFLLLLSIINLLIALGSLLISNIYFINYEFFQQSLRKYFNYINIIAQLLLIPLIFMSIRNQFIFVLVSKIIFGLFICMLIGEAIIEYPFGFSAYAKLYNKGLTTFTIVYLITTIQKAFSINSYSCIVIFLILLPVSQHLTSALIEMKRQTVYQTISKQNQYFELLYIENMFELNGEAQKCKQKEIEYITKFCMHHSHCRQNKCQCKKIGFEKIIKLDQTVIFVSCLFKRSFDKHKSTVQKRIGDFEVLSLKFLTFINKFKRNGPKTYQELKVLFQKQREYSFYFIQMCLFLQFIIQAQMQKDEDYNINSESRSRISNVKLQVSKSERSIVQNLYQMEQVKQNMLPLLIQICQFKSGFWKQIQEGKIATFIEIEKQLQQLQILQQQITKEYNRYCSIFFVNGRTYNVQFLKFDALIQLILYNNARAYYDMEKDCKEILAYERSMSTFEITNINFFKGDAISVKVCIAFGPNIGKVRNKVISPLIPRFFGFADMASFIDYTKGNINPLMPNWLLGVHDEMMQNYIRRGSTQRIGKYFQTFAKLCDQTLIRCQVYLAHNFSPDLEADFSMIGCLKRLEQDEIDPKDESKRLRNLIFKGQQHVLFDIYGNLMGITQGLYKMIDRMQRKLKDNNNNNNNKQDEVKQKSFENDESSEDSDSEVFDVYEIKWSNQPLTIEDFYLKVLIWMLFPFITAEIEQTGIEYLMNGQIPPKGKYPNPIDLESSNTVVSNKETYMFIPEDINLFVQQYNKVLGKINEDEKIQTSNFSLSGKRAQYIESESINAKQQVTVFNEKLCSFFFEEFLRKHQQLVLGNFSRQDMRMSNLNQMTTTIQNSEKVDSSSESEGQLKTRAEQMYYDKFQNYIQRITTQDFNPIPVIYSINYEEFRYKKNEIENKVQMFVIELTVNEQQLASMEKGYRKQLRDTLKQMQAQMKQEKTENLNRGSTNVKQSQNQSQIEVISEDSMSLQGNFSEKVEQDDQISLYKFLSHPSKIEEYCFSDQNILTDQCILTSAKEEQNKLLLTNRKSLQLLEYGIYSAKSNGTENQFEQQSKNNFLKASEKEAASKVVLKLSQKSRQQKFYNELEQKVIQSNQDKEREKVENEDSYLIQLKNFDENYKAINQKINEFQYPNSYKFQNYILFATLLLVIVYILLVSIAVWNQYDYGDCMSLIQILLKFQKTYSLITHGLFRIQYSYDFNITNFDQLHVFYDKQIFQEVDQLINFSNTQKNTINDIDIQLSIFYDIDNSTKLNLSITETIQKSLAQFYKVVENKVTTDYELQIQFIQSSVANLKEIGQLPQLAFDSCYDTKIDKERYYQQLLIIFMVIIFLLVLILQSSQIPMISTLGKYKKKLYMALLDINQLQFAQEQESFDALASTLKKSVYDWMMIDFIVEGKIFDIEPLYLNGFSSDNASTNYIDNQNRTKQEKNKLIKKLKGSNIQQIRYIILLIIDLVVIMSYFLVIFLIIFILTDQLLSGLELLFKYQTLQSSFINVLNNADLVAYKSLSNSSLYYNIISQSEFLNYSQVLQGTEQDFFLQYDNNIIVSLTDENINQKIEFMNQQSICNTQYALDCQSTLVGIYNEQILAYYQQGLKSLFVQVYKIIQQYPQFYQGNQINNSLTDFSNFVETSDHIAYLDYGTDLIITAYSSIVDMAQEQFDIQISKYQQTLIIFILSIGILGLIIKAFLGRMLMRMQKQSIDTCQASLLLLSPRRYTQKSIAKLITQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LGN1</accession>
    <name>A0A8S1LGN1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0370245</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LGN1"/>
    <dbReference type="OMA" id="FKPEAYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF54">
        <property type="entry name" value="CHROMATIN ACCESSIBILITY COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09011">
        <property type="entry name" value="HMG_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="34"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="92"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="174"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8065385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="23744" checksum="A16DF982F28B87EC" modified="2022-10-12" version="1">MKKQILESGQKVITIDDTINSKQPTPVQPIVQQLSAYQLFMKDKKEEGKYSLQVLSQMWKDLPKDYKEQYQERARQLKEQVQQNQLTTPQTFFPLSRLKTILKEDEQKPQFKPEAYQYMLKAIEEFGELLLNKVKDQIKQDQRKTIKEDDLINLIKENQELQFLSCLAIHHQKKKTSQTQNQQGKSKTSGALDKFLENNNGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LWE0</accession>
    <name>A0A8S1LWE0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0450151</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LWE0"/>
    <dbReference type="OMA" id="NDQIRPR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="137" mass="16665" checksum="2E1321A764B7813A" modified="2022-10-12" version="1">MQFDQEFGPWSNDQIRPRSRYNYQHETSLNKNISEIRVVRRISYKINSIKRSQYPKLKINNISPQINTKRSQNYESVKKREEYSEQHALPKLLENKVLLQLKIPRHRHTDMKVVQDIEKIIRRNLFYIPTHKYSIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZB2</accession>
    <name>A0A8S1LZB2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0410244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZB2"/>
    <dbReference type="OMA" id="FEIQMRP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="47"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="584" mass="69491" checksum="AD2172080E04C036" modified="2022-10-12" version="1">MKRQRSQSWEPQSFENSELIRQQSERPINVENSIFSMIVNHNVIKDISIVDTYKEIDNISMHKKHFVNYVIKIKTDYFDYTIAKRYSEFEKLYDQISNILHKLPKFPEKKLIKSNSKKNIAERREMLEQLLKYILRNLNNQMHYFLNFLNIQQQFLKGGFGQNVRLHEMSKDEFGLSDLKEETKLVASNEQEKMVFHYIAKLNERNDERSKIFQKMEKYFFGKTQHLGSQVLKFMLVGDDQRQKGIIELLKNTSKDFQSHISCISGMQFLRKMLEYDFNPYADFFLKVFAEVSIKQLKKMGISFHVCGTNKQLCKQDTLLLIYKYVKVNDLKPEFISFLLEDQETLTEFQQFKEAYQQKKEEAFQFIADSAENLQKETAPIKSNSKEKLSILDQEPSIEQLKEIECNQSIIWKQVQDYDNHLIQHIQGQIVKTVHPLKCSLNRAIELLTSQKHKWVKTMVVRQIVKQIEDNKQIVAETYLWQDKQLFKKVVFFSEETLIINDDNFEIQMRPVFDCQTYEQFQKCDETSQKLQVTYGIYKNGTTTVTIIQNLFDKVQQLQYTPVLLKEVDYFEKTVQQLKLLQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M1U5</accession>
    <name>A0A8S1M1U5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0430116</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8068944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M1U5"/>
    <dbReference type="OMA" id="KWKFKEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="38"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8068944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="519" mass="59831" checksum="28011B916D1E50FD" modified="2022-10-12" version="1">MNGGHRLKFQISFSVDEVKPEIITLNQFDDALKFEDRYEALQTLGQGAHAVVKVARKKDTDEIFAVKIVRSGDQEIQNNVKRTFNNTRCLRHPNIAQDIELFINEKMETSYLIMEYCAFDSLESIIKKRKLTIMELKIVMKQLLLAIQHAHQKGICHRDLKPDNVLVDLSENMDQSEVRVKVVDFGVSRRFQSKGQEIEMLTKTGNIFYCAPEIYHKSCYSKEVDIWAIGVIAYQCLFQKLPLHSDEYQDFVELLRCPDKWKFKEHLKELEIPLQNLIIGMLEYDSQKRITIDDALRNSFFEQQQRESTPLIISTNLENLIYNNNKHMKQLQTSLAINNNYFGNHNYNLGNMIQKLQGQNNQERVDVEDLTKNFGNIHILQKQNEKRGPIQLMNSIGSSNVLMSKLGSRQEISYQTQSDALRKICDIKGSADPFGDFAIQQSQELKEQQESNSQQNNNTQKLFQQLGSQIESSINLTAEDHTKVINQLDSLGVPKRSSVLAQFLGDLGIKEVDEITEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M2K0</accession>
    <name>A0A8S1M2K0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0520087</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="GESINCL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="39"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035940046" description="EGF-like domain-containing protein" evidence="4">
        <location>
            <begin position="18"/>
            <end position="3627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="3084"/>
            <end position="3104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="3445"/>
            <end position="3462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="3468"/>
            <end position="3487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="3499"/>
            <end position="3519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="3525"/>
            <end position="3547"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="16"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="97"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="127"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="162"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="226"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="267"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="354"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="400"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="550"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="588"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="632"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="680"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="727"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="774"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1202"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1298"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1634"/>
            <end position="1665"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1753"/>
            <end position="1791"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1792"/>
            <end position="1835"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1840"/>
            <end position="1878"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1886"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1931"/>
            <end position="1968"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1973"/>
            <end position="2012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="3196"/>
            <end position="3215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3126"/>
            <end position="3153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="3197"/>
            <end position="3209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8074117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3627" mass="416913" checksum="4CC6C19C11BC30E8" modified="2022-10-12" version="1">MNYILIFLLIGYASCQCQEGYIWTNTGCRLCQNFCSSCTNGLCDQCPPSYYGDSTHCYSNHQNKLGCYIGCTQCTDYQVCTSCQSPYYFLVQNLCQRCTSPCETCDDQGACLTCISGYMVVGNDCQKCQSPCSQCSGELYKCTSCKQSPPQHYYLSGQNCILCEFPCVSCISKNICTSCNQGYYLNISQYCIKCTEPCLDCNGPTNCLSCINQEYYIDLGDNQCQICSNFDSNCLTCQAYNQCSSCKPGYYVQDDFIGSQFISTCNKCSSQCLTCSNDSNECVSCNNIYQPPTCKQICNSSQFQDGLLCYSCASQCQTCSLKSIQCLSCSINRISPPLCPCKETYFDLNGDCTPCISNCKYCFNAISCIKCQDGLYLQEGICVQQCDQLYYINRDSNCIYCNINNCIECNINGICDQCQQNYLLYKNQCYSQQCNQGQYTIDNENCLNCDINCYNCLNQADNCVECYNDQYLLEIDQINNCVVECPLNYYIHNNQCLKCNSNCQQCSQENVCTSCIVGQYLLNNICYQNCPIYYYGIDQQCLQCPNNCETCYDKYCYNCQNGYLFYQNSCLEYCPEGYYGDSNQKCQSCQNICKACVNDYECISCSAPLYFENNTCIGNCPISYYRDLVDNRCKQCIDGCEICENETFCYKCLPNYASFKKRCVIECDIGYYVEDQYCRQCDFDCYTCYGPTKFDCIVCKYGLKLYKSMCIEDCPKNTILKNGQCIECHETCDTCFDRGNQNCLTCKLDYILLNNGCYAYCPSQYYKNKGQCLKCQDNCDICINNLSCIRCLNNYYYNRGNCLSVCPNGYYGYDGVCEKCHFSCATCNGALQTNCVTCTQPLVQQNNECLPGCLNRYYKNLSNICQSCKETCAICTNNDNCSECIYNYYLHPLTKDCVLICDNGYMHNYIQRKCQICATGCQSCFGQSINQCLSCFPNYFYYKNQCWYSQCPISTYDEQGICIQCDQYCMTCDTQQSCLSCLNGYFYYNQKCYQTCPDGYFGDSINKQCSQCKQNCRICQDADNCSYCYNHYTNNGQQDYFLLDNQCVAICPINYNYFLFECTQNITPIQIIQNVAEYCYDDPNTYLDINQNLCIKCEQSCQTCFGPYRTQCRDCVNLIANRMCVDQCGQQTYQSGNECIPCHLSCKYCVSNNRIDNCTHCHLGAYLQLQENQLYGRCVNICDVGYYQDYDNKINGQIICKQCSSNCLTCIYFLQCTSCVEPKVLRNGNCVNSCNSNEFVSKIDNKCYQCKAGCSTCIDSLSCTLPFIGYSQTLYLQNNQVYASCGEGYYTSGLLCLECLDGCRICSDSNSCSKCNSSFILFNSNCIPQCTGKSYFDNGQCSPCHINCSFCYNGDENSCISCETGLKYIVIYDNVDSTYKSYCQDTCPQNYTYDIQQRICLSNSCHNSCLTCVGDQYNQCVTCSNNQILSLMTSKYGICQSTCSVGMYNDNGICLNCNDFCQECTSYSSCTICKSNFYHLNQQLCVIQCPLGYYQNIDTYKCDQCDSNCYECIYPNICINYIQNCATNQFLYQNTCINCHWTCLTCDGPTEYDCTSCGYINPEYRYSYYKQCLLSCPKSDDAYKCQLCPENCEMCNSNQECISCFIGYYLLFNTCVTSCEQRYYANQQYQICEPCFTGCLECVGPSHQQCVLCEPNYLLSDNQCLPYCPYKYYYNFLSTKCEYCDDFIYKNSCVIECPLQYNPINNVCVQCTNDCTSCQNGLYMYQNTCISNCIIGTYNVNGVCNLCDIQCYSCQSQSDNCQQCSSVTRYNAPFCECQIGYQQDSPNKDCIKCANQCHSCINTFNNCIQCKSDRISNPPACTCPYGKYDNGVNCVPCRHECDDCLSNTLCLYCKGDRVGNNCLCRLGYYDDNSSLYCQKCDHSCTTCTKYGCLECLGNRILNNNNQCLCQQGFYEDGESINCLKCHFKCQTCDINQYNCLICKGDRKQTPYCQCLDGYYDDEVNIDCQSCPKECYTCNKASCLNCKANRFGSICDCPPGGIDRQDVGYMHCETCELGLPIIQMQPDLTTLIIKFGKQVTFNFDLGCSQIIDVSLLGVNPKCSLNQLSQIVIQLGINNSIEIGNLIQLFTNIKGIDCDLPYTSIFSTNILPPYIINDSDVMIFGPTQVSTCEVTEFKVSQYFFDGHNGFKMVSWNLESFSPYNQLTEQQIFGILYEVNLYKSTVVTIPPNILQQNTIYKFKYQFINYLGQNQIKYYQVQCLPLNLPIISIQYENQQPQVYYINNRIIIKATIQQQSSCDSPILNNVNAFYKIQAYYQNNLIILDKQFNFKHSSQKYVEIFIEPYTFTQSGYYQVFLMATFDQLNIKSKSMTFKMLMPGIYAKIYGGNQIHSFGQSFNITAIVKDLNVKELQTKDIEYSWTCINVALQRPCETVDKQPFTIKNQQIVKIPSRTLAPFNSYQFVMKAQKKNLIQVTSSIMTIVDVEISTFSMDTPNIALNIPILFNQELLFEVSSLQLTNLHGCIIYDFQQLSAFSIPYNTFAITLQNLLKQRYDQIKLQIFSTDSIYFNPLLTSVEITTIQPPLNCKLNVSPKKGLSFDTIFKLEIQSCVDQQNPIYYKFLFYYNQTQYLQDILMNSQMNADLIIDYQEFNIYSTILPKSTNSHSIIIAIIKNQLGAQTNLTSTVIIGDDLAEINDKVIQFESEINSNQAKVLQQNKRILNDDILNTFTTLYNQSKYLNEIQRLSLLNILTIGVEQYTTQSNHLDDIRSILYFDLQSKLDLPLISETTKMQIKKSINRIIINNTVYLQDFSQIQVYLTNLNQSLSKSINDVLFIINSISQNQVGYRDYQQKELEKINMIHQLSTLDYLLKSQSNFYYDYFHGNISLLNETMYKSISSNFESILNLLQMLKYGLLLVQQVNDDPITFSGSSFTLIFNRLTTEKMNEYLSEIEFVKLVDKPIDNNTHFSQSLLLNFSYIHYLENPFVLDSSYPNNTHFTTIQSIEPTLQNGSLLIPTQPITTKYNVTILRQKRLLQSYQDIYLMDEQPNYVDFVCTNKYFTQWESNFCKTSIEIDTQSSLIIACDCQYFGPTTISSTLADIFEIDKFSKSFSLEAIEALNKFPFHRSVIFYCLVIFTILLILFLPYGIKRDQIDKERAMPQHMIESEYIRKLFEDRQKELQKEMEKIKEERNKEWKEFYEKQNLIEIVTDDKEQKQQSLDKDEQIQEVFISPDIDPDEMIDQQQQDQSDNSNNFNLDMSHDSDEEFQFEEYPPINISKKKNKMQAPSPYAKFFQLKNPYSLNNTIVNRLYLHSSSYEESLQNQQQHQNQKNLIHERHSSRIQSDQFSVRNSENIQQIILKKISSNQSKNYNNSLNSQAKLNDYPPLSPNSSSPIGAIYDSKQIIQPNQLGQSGSQYESHVNSNSTKVAKTQIPFRKSQEHILFLDHEFYEEDNLSIEEKEKIIQIWYLEKKRNKLLEEGFKLRFTLKNLFLFISLFQSVLSIIAIFDKKLPRPIRCCLIYMTLLTTCYINIFFQVELNPAATIAYSILSSILSMIELIILTVLMPHQYILIRIIGWLSFLVLTGLFSYLIIASMAFEAQDSGGDVSRSNQWGINFIISFITMNFFTDPLQLMVCFKIIQWSFNNASPHLQQIFILLCMNKHFNIYFDFIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4D2</accession>
    <name>A0A8S1M4D2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dynein light chain roadblock</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0510088</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
            <location evidence="1 10">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GAMAD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4D2"/>
    <dbReference type="OMA" id="NNSTIEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000009">
        <property type="entry name" value="Dynein light chain roadblock"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016561">
        <property type="entry name" value="DYNLRB1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10779">
        <property type="entry name" value="DYNEIN LIGHT CHAIN ROADBLOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009998">
        <property type="entry name" value="DLC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 10" id="KW-0243">Dynein</keyword>
    <keyword evidence="5 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Roadblock/LAMTOR2" evidence="11">
        <location>
            <begin position="6"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR009998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8073502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="12031" checksum="927B3C5F5FBF6B86" modified="2022-10-12" version="1">MSDVDIEDTINRIKNHKSVQGIVIVNHEGTITRTTYLNEKKEEGDTIAKSIPILAQKARSLVRDLDPTNDLAFLRIKSKQNEILIAPDKDLLLIVIQGPKKQGEDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9B3</accession>
    <name>A0A8S1M9B3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0480110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9B3"/>
    <dbReference type="OMA" id="WIGICHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="42524" checksum="29E082F147BA3801" modified="2022-10-12" version="1">MESCQNEKHIGNLLFGYCSKMNCNSNLKTFCDICRCYHDEHLQDLHQLDSLDDIKLKNIEYNCQLLNFFENQKRFCLEIQTPLMLINYNNNLKIDYQNKEVLIDQIKQIVTIQSILNAVLPMFQNINKSCNQIIQEFNKLQQFNYNLDIKKQEIKEKKNILKKQKIVRKQDQKSKLLSEKQNITEIIQWQNLQIHFSQKFKYKYIKILNNGLIAQNEDDRGMVICEPMLPKKGQYRFAFGINDNAYLIWIGICHKEYLIQNNYKTNFSIQSDNKNGVYLINNYGVTNSHLNPDLNDQTNSFFFELDDLIIVYVDMDIGYIKWESKKSNNQYSMQFDTSQDVYPCAGITAFSPIFIVQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAT5</accession>
    <name>A0A8S1MAT5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0580153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAT5"/>
    <dbReference type="OMA" id="QKIPQTA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="288" mass="33609" checksum="646DC6C19D867CB8" modified="2022-10-12" version="1">MTTNWQQSYVPIRLNMNEDDDPFEEEIDRLRQQKEIQQKLKQQQAAIYDHNKKAEQLRLQIVKDVLPKHKGLQLAYDYDGTCMLAKDFAQVKNNQRVPEIHKITQVDTVFQPQKPVQQAVIENEKVKKEQTRQSKAKTNPPQIFEASVMKPFDGFTPNQGVKLIYQDQNLEIVRQQDIQNWGDGQRMTKKQFEALKNEGFKPSTYSMKQQMLQKINSDIVEQQEPKNTPMIAESTNQINELKISQSYTNLKQLQTQQTIQKIPQTAQNRKQGSKINVVDKQLLDQLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MF10</accession>
    <name>A0A8S1MF10_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0570148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MF10"/>
    <dbReference type="OMA" id="QYYINNH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="547" mass="64939" checksum="BD28B0BED4F93EC5" modified="2022-10-12" version="1">MNFIFYFCLLQNVLNTKIKRPQVSIFNETLTPSTYISESQEVIEIQYENILDIFSINLHKRISGEINDDNMNLVEFEKIDHYNNEDCQIIQRNIDVRMQLKLWLDKTGIKEGKYIMDIAISEKYSLAFIVRNDYKLFSVNFNQSAYGIYLRTFDYSFENTLNIKEQNKDETPYLIIDDQSNKVFIFTQHQGVSFNIEVDAHKEIQFNFEDQIQKRDKIYSVHYNNKYHLVFVATGYQGVDIYKIINGSITLQQNLSSQFRNYSQIVDIKSDGEDGLFILDREQGLNFYQIRNEFEYEYQFLINVPDSKSFDFNNNTFFIIAKTSKKQDYVLEIFVNKQDTQYYINNHYFEDMIINDVSVLEQYAILIGNNKHKIVYHSIYSGFIEQKIVSHKSFYEPQLIKTKKFKQNRIDNPNLQSVAVIGRQEFQIINFIVNNPIIKCNMNKQIKNQFLAEINSTRCINDHIETNTQKQLTLCKIYHSFTIQKQNLVQNQVKNVLNIILFWSFVIVFYLRYFAKSRKFTGLKIDQSIQLNNGYDSEIIEQIQLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MFN0</accession>
    <name>A0A8S1MFN0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KOW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0610201</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MFN0"/>
    <dbReference type="OMA" id="QTICQIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23702">
        <property type="entry name" value="eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000104">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_eL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002784">
        <property type="entry name" value="Ribosomal_eL14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01929">
        <property type="entry name" value="Ribosomal_L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="3">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8079237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="375" mass="44012" checksum="E9EA27BDF21E65C0" modified="2022-10-12" version="1">MRDLRLIVIKQQDKNQKYRQYNQMDRLLNYNYGILKFKKIQCCKQIKYQKCIEYFNQMIVEYLIIFNIKYSNSQQETLILKSIIEENCDQNNIPLIKNKYDLVSNEEQSNYYQSLLGFVNSNKFFSFIQRSVKEGNRIKYREMCQLFTDLIQKIIDKGLYVNQQKNIKQCTIKQCSKLIESIIQKRQVLMLNEYQQIQYINYILHSLFKKIKRIFKQQKKFVLLKMVLGKFVEVGRVVKINYGPQEGRLATIVEILNDKRVLIDGPTTGVQRQVIPIRRLTLTKFNLKGATRGARTGVITKAIKKSDPFAQYAQTVAAKKVAKKALRAKLNDFDRFRVMILRKRRSALLSTQLKSLKKNTGGKAQAQAKGGNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MGG8</accession>
    <name>A0A8S1MGG8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0560243</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MGG8"/>
    <dbReference type="OMA" id="NCVAIEK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF14">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8076625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="282" mass="32800" checksum="A002F1A51AAA1727" modified="2022-10-12" version="1">MKNAVLILSNKIQLKINKIQCNQNLRFMQLQDLQVFKMFIINGKITQGLDLYPDNEYIIQPLGSTIVVRHIITRQQTFQDLSLITVSRIGNYVASGQTTYMRFQADIIIWDFKDRSVIHRIKLHKILIQSLSFSYNELYLASLGGIDDKNMLIVWDIKAGKALYGTPNRDPVNQVQFYNQSDGKMIAVLNTGVEILTIDKQNKKIQSVDVNFGNFKLTFNCVAIEKNDKFCYCGTKTGDVFEVQMDMVIYKDWHQSKNYFLKELIFQVYYIMVTQQLKQEMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MIG0</accession>
    <name>A0A8S1MIG0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0610251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MIG0"/>
    <dbReference type="OMA" id="YWVAVAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="102" mass="11670" checksum="9329D76257C46FF4" modified="2022-10-12" version="1">MDSNLYVKLSPQGTQKHKELESQCLMKFIYWVAVANISGALGGQLMMYIPMSSRQKQMRFRNGVFISTALLLSYHGYKLSKRDLRIGQKEIIQNKDNVLSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKF1</accession>
    <name>A0A8S1MKF1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0630051</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKF1"/>
    <dbReference type="OMA" id="AENIEWE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00124">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF706">
        <property type="entry name" value="DILUTE CLASS UNCONVENTIONAL MYOSIN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0518">Myosin</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="5">
        <location>
            <begin position="38"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="3">
        <location>
            <begin position="634"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="963"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1126"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="149"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8080019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1154" mass="136285" checksum="4E18E6E83FF618A9" modified="2022-10-12" version="1">MSFDDEVWVEDEKEICKIERLNEAQAATKFRVDPLAKQGIQDLLCLSDLNEFNCLHNIRIRYLKELIYTQIGSSILVSINPYVFLQNIYNQETIAYYKIKLGQHQAQLKTSVRENEFEPHLFKIAQLSYDQLFETKTKELRICSMIISGESGSGKTESTKILLRYLAAESRSGLFRTIQHGQTIEQQIFASNPVLEAFGNAKTARNDNSSRFGKFMHLYFNPDTKRVSTAKVDNYLLEKSRVVKINKQERNYHIFYQIIASQIPELKLKSPQQYEYLKGGDLSYQRNEKQEFEETDKCLDNLNFSPDQKKYIYQTLAGLLHLGNIQIEYDSIKSKLKLDDSLKLASQLLGIPNQQLEDLICKVFTSVGKEIVTKNNNLESAQSSRDTLAKHLYEKLFNWLINKINEQLLNIKSLSFSNQQQQPQLTQYMIGILDIFGFEIFTDANGLHSNSFEQLNINFTNEKLQQHFNQQMFVTEQKEYDQESIKWQQIKFPDNKKIIELIENSQISIYKMLIDQTIVLNRGDKEFLNSLKQLPKDHLVLVSDLQTAEIEPRFDKKLHRIAKFDWFCLKHFAGAVAYNVTGFVEKNRDSINSEVFKVLPNSTNPVLKEIWSNIQNDNQNMKQNNVITKFQNSLKDLLNLLNQSIPKYIRCIKPNNFKQPLTFDAQEVKRQMTCAGLMEAIQIRKAGYEIRQKHIDFIKKYQYLVNKNPKNIKNIEEMFKLLGENQQIGQFLKEQQNQLGFQIGKTKLFMKQDFREYFDNKLIEFRLKYIMKIQKQFRIYRKINIMKTKLKELHKKLIRIKRAIKRFLFKKSIKQRIILRQKINTLISKCIKCSSKMNIYYSLNQWKNRIQEIKAIEQAQLLDDVEIIGESIEFTKILKIDSYSQESDLTNKQQQQEELQQNEIKQQLETKQQEQTQILKQEGNSKGSFLKQTVQLNTQEKIKQVDQNQDQIKLQDIIQSKEMQFYEEKINTLMQEIDDEKKKRIQLEQENKQLNHSLQDVQQNDIAEDRHREQILKLEKELFKRDQIIHDLNVKLNDYSKQIKQSSSINRLSEVQDMKISQLDGQLQKKNELLKLYSDLTKSLNKGWRMKILENECLFTYFNLKTYQQKLPIYEQFQITQIVETGDLLQKQIKDLQQQISQLEQSLSKSQMNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLJ2</accession>
    <name>A0A8S1MLJ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Senescence domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0650065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLJ2"/>
    <dbReference type="OMA" id="QEQPREM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009686">
        <property type="entry name" value="Senescence/spartin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045036">
        <property type="entry name" value="Spartin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068:SF43">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06911">
        <property type="entry name" value="Senescence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Senescence" evidence="2">
        <location>
            <begin position="145"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8081130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="332" mass="36793" checksum="8B362109F8E13AA1" modified="2022-10-12" version="1">MQQEQPREMAQVVSIAENAQFFRLIQNSRTKVFQGTVKLAQFQDSKTHFITIVSFCQNIVPGTPFVYIQSESGLTLIFPHIDQGLNGIFIPQSDLLNVFQTALNTVVSMVPFDQAIQIQQFKDTIELHCKDIATNTLDNPNDAASYIAKGGDYLKKGFMFLGTMMATGINKGSQYLNDKIQPGQEVQVDPNTKTKFQATKEKVSEVFDVTGQYLGQLFKPVAEKTKELTNELGHKIDNSDSQILKQSKQLTSATWDAAGTALSGLGTALCQVGSSIGNGTKQIVQKKYGTEVVNTYLGGDQQQQQVQQQQQQQQQQLDQYQPLIEEQPAPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNC9</accession>
    <name>A0A8S1MNC9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Obg-like ATPase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0660136</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNC9"/>
    <dbReference type="OMA" id="VLRCFDN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.300:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00092">
        <property type="entry name" value="redox-regulated ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF11">
        <property type="entry name" value="OBG-LIKE ATPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="5">
        <location>
            <begin position="22"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="6">
        <location>
            <begin position="298"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="31"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8081720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="389" mass="44056" checksum="12D4662A616D2ED0" modified="2022-10-12" version="1">MPPKKEQQEEKKNKLGRPSNTLKMGIVGMANVGKSTTFNTLCKLNVPAENYPFCTIDPNNAKVPVPDDRFIKLCQMHKPKSEIQAVLSIVDIAGLVPGAHKGEGLGNAFLSHIKECDGIYHVVRAFEDENVCHTELSVDPIRDMDIISTELLLKDLEFCNNRLAETEHVIKRNNNKDAREEKEVLDKVKQLLDNKKWVRTGDWNFKEVEILNKYYFITAKNVVYLVNLSQQDFMTRKNKWLKGIKDWVDANCPGDIIPYSADLEKAIFEESQAGTITQERQKLSMLPRIIKTGYKALDLIYFFTAGEDEVRCWTIRAGTKAPQAAGVIHTDFEKGFICAEVMKYEDFVNLGSITAVKAEGKYRQQGKEYVVEDGDICFFKFNVGGGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQR6</accession>
    <name>A0A8S1MQR6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0680165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQR6"/>
    <dbReference type="OMA" id="NGSKMMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="894"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="144"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="364"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="511"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="639"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="755"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="894"/>
            <end position="905"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8082798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="997" mass="118779" checksum="2DB24D625BBE8398" modified="2022-10-12" version="1">MIPYPHIIQPTIKSANQSSGFKEGEEKFQHSTPKYVLKVPIPSLPTLQQAPQSDNKSKSGHKENNFSAMTFDQKKWNPDSQGSDNKCVLSQSAKKVNTENDDGSSIVEKSFTEAAQKILKIKNLELENKVSLLIIENERLTTFLNDQFQENEKLTNDLQNYQVSLQDLSKNYEDKLNIVLLENEKIVAINQTLTMKNEQMQNQIKRLLNEKDEEINNNRKEWAGKYQQQINQIDEMRKSEINQIDNRIDKLQFEKSELQILIQEQQQKLMEFQIELKVRDHTLIDYENVKADLASKIHKIEMMSQTILAQEKRINDLEARLDIMVADNKQLHDVNEQLNQTQRILQGTLEDRQIEFDRESHKQKDFYELELRNLSNKNKQLIEEIKLKHQEQLQTQRIQIENELIKELEQYKMELETKQNELNNNLLIWQEKEKNITQENKKELEIKDQMISTLKFSNQSLQEQMNQLQTLINKMQEDFDSQFSQKETECVKMAKNIHLKDQAQKQMSLRLDEICKQVKILEEQRDDLSQEMKNKNDKVRDLEMKLDIVVKDNDTLEYQLKNTQTQLQLLEQRYINLEQTSEQKIEDIKLSYSLEVQAQIEQCITQYQQTIKYEQDHSEQIMKNLKKQQEKAKHFELLSEQQLNDIEKLNKRIEELIDVCEQNGSKMMILENLLKERDNDYDVLNQKLQSITENYELQIDVLKKQMYDMGIQNEDLAQNLNSIQETFIKQDELKKNTMNDLAEVTKLKIDQDQLIKKMRIQIQGLDEENRKNSEDSNKFKNQVYQLQQEIEEITRSMQLNFADKEQELQNKYENTIMDLTQQLNNLTIRFNDLVRKEKDNRFRLKLLKSNPQMKLPSELEDENCYETMKKKMEDQSQYLQRLILENDELKQQQYSHISKGSLNEQQKQKSQRQSMNIIKDYTTQDTDRTIRQNEYQKSMNPFKQQPVKVLPVMNNSQNILEQKYASNRPIDRDVYLGQTQNTRTEPHLYRKQSLQRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MR84</accession>
    <name>A0A8S1MR84_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0580081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MR84"/>
    <dbReference type="OMA" id="KEQHQCR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="480"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="546" mass="66067" checksum="024730685737DBBA" modified="2022-10-12" version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sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSK9</accession>
    <name>A0A8S1MSK9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0630073</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSK9"/>
    <dbReference type="OMA" id="KCIYERQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="420" mass="49393" checksum="78842B4304F41E8D" modified="2022-10-12" version="1">MKSIKTKLVTNTSQIETTINNHSISSRKIPKLPYLYTKHNQVLSSSRKLVLEENGVLIQLNTLKKELIKCIYERQLDEALEITKKLVQQCIGYDLTIFGKSLHLLADMYISNREFNNGLYIYNVLRILADIQNNQTLKIEALIQMGDLCKLQQSYHLSKLYLKKALQYVWYLNDTENEATIYDYFGVLYYVQGELRLAQEYHDRAMNFIKESNDSAAKNHSVESIKTYIKKINYQSQVMNNMVLSKLGLIQGNETEIKLTTQLDIGTLNNQILSDPEFQVEVATPNRPQRCVTKNGVLQNDVDMFDNVKELHERQLKRKFFEKKIPVMNQTKSILKQTIDEQLEQRMKSDQNKNSVERFKQYLQKFHAFKNYPEKLERVNINHLSPNRNLLGFKYSFKPMRHQLLELISDIEQYIYLHQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MT80</accession>
    <name>A0A8S1MT80_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0630022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8079965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MT80"/>
    <dbReference type="OMA" id="KCPNLFC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="348" mass="41383" checksum="4796B50A441BB344" modified="2022-10-12" version="1">MEKIQQLERRLEEKLDENQKLVKDIKSKELYIQDLQAKLIQLQTEKTQLEIKKKVQDDQITKLTYNLKEIEKELWKLKSIELNEQRQQNFYQTQSKSRLIIQEKSNNNNIIDIEGQSFGKNFKQPNNNNDENYSFLEYQIKQNKDLNRDNQILLFKLQNVDELSNQIKNSQNLINQNNEILQKIKRIVNNSNYTSVTLIQQELKNIVNQNETKDFFNRYFKCCDGQNELVLCKQRSCIYHLNCLYPKLIKQGQLALNNCDCGQPFTNSILRKVNSVESKCILDQILDRQLQKLISQMIQGKRFLLYKCPNLFCNFQWFFRMDKSSSNQGSLSYCPNCQINVYSEIQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N187</accession>
    <name>A0A8S1N187_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0710132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N187"/>
    <dbReference type="OMA" id="PHEEEFN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8084011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="263" mass="30911" checksum="49375EFEF66830BB" modified="2022-10-12" version="1">MSNCIIKEEEINVHTPVSISKTTLYKLNKKKDLEENLPFQIAYSKAVSKFLQALHILQLAQNHSQNISVPSYPFVLQYSYNEFLVHLGGHKINVQNTPILNDFQQQLHIQQYVPRLISQQSPSIKLTIQKRKGISNQEQKDTKNIPKNYCKAIITFASKHQTLCHQILGDQLKVVKFLDRITVYKKKLMNIRTFSGLLSQSEDPVEEEFNKTFRILSRIFIKKYAINYIFNSKIVQHNWHLRYRQQIYKGIKNPKNFSHIKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1S5</accession>
    <name>A0A8S1N1S5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0770097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="WTIFNYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1054"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1158"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1372"/>
            <end position="1389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1429" mass="168921" checksum="01B6583743DA2D58" modified="2022-10-12" version="1">MKEVFQKLTNYLIRVYLKNEENQIVFPPYVILLFRVQQAFLSLSYIYGLSNYYYIYMISIYARPQQYFIDSEIPQYIILMLSVIYIFTLLTIIPYNNIIGIFGQLLRSSMLNFFFEISKGSISIITHTLLVLISFSETLIISGTLNVQTKNFQHTRFTNLDLIVVILNYLLMIFYSYGFPQSIIVILAIILNILRIINQLLFSSFKNLITKTILLTIKIITFFFGWFWIAQLQIHLSAEIVIIPLIYKMIYTYYQNQLKNIVYNNQKHIPCHIISLLAFLENVKDIQLLYVSKPKKPRNRIIYSSFLIYQNKNYQAFLELNSIQDDQLSLIESLKKKIILQKCINNIQKIICGTNKIDNIAHTIKLLLNSEEQNYSIQNDIVEIVQNKIHCLEQFTQHQSHNSYENYHNIIKKIINFKEKLEKQYKEFPCEKTQGILGFFYGEIMNDYLQANQLFNLVAMQDEKIKKITLNQDIFSNKMIYLILKFDGKLIIKRPSSDAAQFLQLTNQQLKGLELQYLIPKGVQEIHDDLVLNFLKYGKSKYMRQLNQCLLYNHEMEFMSSVELLFDINFVEELNFIAFLQPPANQTLNLILNEQHLIKCISQQFIQELGIEQQYKQYQGNHINKLIPNFPKYFFDKQQIENTEIYVEKQIDEQSQTSSHPLVFITTFNLHFGIVHDITKYFIISFDYFKRSPFYFCQNQSISVPFMSPLIKKQNHCSLAIINDESTIHVPYQEDEVMKNHYIFQLNQQDFNFLDNTPTIQDQKKQQTKLLSTLDYTNIKSTTKQNELLLAEQKFFSKPKIKSSISNGDQVIFMQEVQSSQVSSLQGVRNSKYFRQFEMIAKIKDLNSFSKLHRIFLASIILCVVFQFIIQTIQIIELNFNLKDLVSDIDILQIKNFVFQPLETFLLTRWTIFNYKLLNDAGEISNLEYQNLIQFPLSNLNLGYDSLDKNLKQVFNRPALQNFLEKTYIEIYEYVTTNQGELYNLNLRNSISILKNFQYSFKMAYELDGGIVSDSPYVYFQYKNYLTIKQQFSELNSIVLDQTLKRSLQIQSKLSIITFICLGALTIQYSFSVIYFILLQKIVLKHYSLTQYLQIQYIEFEITYLKKLIEGVNQNQNLLFRYQFRLDNKESLLDSTNLKIYQKKRNIKQLSILNYHDIYYYLFFFILLLLTAGNASLTYYEGWNYLEKYPETAKFYKEVSDVGTDVPTMFAQRDVLYSRKAIIPYFTQNEYDSLLEEIVESLNRTKAFIQDDYNFDKYVVSDSFKGFFTQIQNDSLCDFIPLELKNKSNNICQIVLNQNMKRGLQALLIYIINHITTDMEINQFTSRSQVSYLELEGAFLVSNIIKVVNDKFNIDLMDQTKYFMNLINMHNIIMFINLFLIEILIITYIKNKLIEKLHIAQRLTYIMPSKSIILDSNFERALRSLLKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N637</accession>
    <name>A0A8S1N637_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0740034</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N637"/>
    <dbReference type="OMA" id="ANGRFMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015671">
        <property type="term" value="P:oxygen transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00454">
        <property type="entry name" value="TrHb1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002779">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019795">
        <property type="entry name" value="Globin_bac-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01213">
        <property type="entry name" value="GLOBIN_FAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="15"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8085236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="542" mass="62430" checksum="7B3DBF4665AF3D75" modified="2022-10-12" version="1">MQAEIKNIPLNSGKYTTQQRLGSGSFGEIYLVTTQSNEILAAKFEEAKSKHPQLVFEAKVTKALQGGIGIPVLHWFGQEAGLNVMVIDLLGPNLEDLFNLCQRKFSLKTVIMLADQMIQRVEYMHSKSFIHRDIKPDNFLIGLGRKSNTVYILDFGLSKKYRDAKTHQHIPYRENKNLTGTARYASINAHLGIEQSRRDDLEAIGYVMVYLLRSYLPWQGIKANNKQEKYHKIMEKKMTTPVEVLCKTLPIEFSTYLNYCRSLRFEDKPDYSFLRKMFKELFQKHGYDWDYQYDWCLAINGQKSNTYNNGKITIQVNANQYDADENQPPITIQINEKVQDKQVVESKIIENKYQRVQNPDNDLDQTEQIARNLMGNEQFNKQQEQIKKNLMGDSEIKLSIPNNTNQKNQAPIETVFNPRVVIQKKTIYERLGQSMAIDFTVDKLLQKLQNDPKLKSFFVKTDMKKLNSQMQAFLTMLFGGPSNYTGRDMLEAHRGLGIDDTHFNLVVKHLSDTLVNLALPEDIVAEVVEVCESLRDDVLGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9X5</accession>
    <name>A0A8S1N9X5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0700043</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9X5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="302"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="347"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="74"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="315"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="392" mass="46778" checksum="B6393FD9AC033E80" modified="2022-10-12" version="1">MQFHLRHSYFIENFLSQFSVDKQNQVVEYLINMGIDLANKIGTRNTKELYQQLKQLSSININQQITENIKEQTQGDIKNEIKTIQKQLRDLNDLIQISQRENQNIQGTHKKQKQSKQIDSNRYQQYEQYEHIDVQPKNYQNQHQNYNTQKSQSKSPIKYNDRTPHKYMHSRSHSQRNLTPKITESPPPPQIVYIKNMHNKFDIKSQFDDRQIKKLMYGNSKKKDRSRYINKGPSPQQIVYSSQTSGGLSYRQYPGQVIHHQMDKQKQNTSPKKLPVYLQNVESKIKPLVDQDKLQYREIQKDRGSHNKQEGTVKQFNNNSLQQQNSGETDTFFDIQQYIQDQKKYQFSPVQEMNDDVRNEDDDVSDTFSNFSPPNEEVKEFFQQQQYRQQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBG5</accession>
    <name>A0A8S1NBG5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0780151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBG5"/>
    <dbReference type="OMA" id="QCEKADY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="50"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="219"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="583" mass="70466" checksum="289BA3B6ECD9568A" modified="2022-10-12" version="1">MSHKYYSISTKVSSSDNVKIIRIQSNKNHKSHNNLINPNKIDPETLSHQNNQLKSQINSLSDEVQVWKLKYQQLQRDSTQNLMFDNSKTFLISLKQQLKKLESDLSEKIKQIEEYKKMINVTKYQELQVELFNYQQQVLLQQQEIKDLREQLQENQTFKIDDLYQKQEKIIQKLSQKNHELKSENTTMKTERYFQNGQIESLKTQLIIKEKEIKRLVSIQKQLEENQQFQNSITKSRQQIFRESVENAQLQEQLKNLEFRYQTDIKDRDQEIIDLKQQLIEYEELKKQLEKYQYISQEQKINQFDIQKVESRQDSTRKLKISLNNSGISQQQQNYSVLRQSSQQYKQSIQMDLSKIQNTEFTKYCNENDESEKQIQQSNRKQKKVIRVNQYDVAQFGLEIKFKLLSKEVSIQEVEMLFEKYDQQIKIHELLDLLLQEPFKIQSYEQRKLIARYLIEDNNEDYVVYDALRSNEIQIVLSVFKQLIGKYELFTKKEIYSIENELKLIFEKYQFQFNDTIQQLIVKKKHLKPGQCEKADYEQAIKFSELNLTPRQLEYIYFINYTFFQDLYIIYYQNVMTYFNKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBP7</accession>
    <name>A0A8S1NBP7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0790102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBP7"/>
    <dbReference type="OMA" id="AHEERDT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="314"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="454"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="784"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="907"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1116"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8087746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1231" mass="146624" checksum="887E04C4DCB7B8A5" modified="2022-10-12" version="1">MQSFRNATILQQERPRGGSVNAKSFLKNAQNINKTESQSFLLQGNNSKKTFESQIPNKNQSSQHDPHLSYLNNLQSTEKLIKTQKLSTLTTKFSTQNLKYLSPSNQNTTKIDIPSDNKQLFIQTARVNSLLQEISDIKLQNEKVKQFYQSQIHNLQNTQDQLKIENISLSNSIKGLTQQLSDARQIMQRIYQQTDLEELNNSFDQIDFNNNPNIQQALYQFQSEIFQFIYEYQNQTNQKIQLKQQQLNKIIIKLNQIIQKQKGYDHKSIINDLQQTNNNLLTKIKSQEVQLKFVHQKSTDQISNQLEEQFNEFKKHHQSIINNLEQEKEIAYEQETQLKKEFQMRIQKLEQELSILLDENQKKEQLISDLNDLNQSKVLQIQKQEEIQQLTHKLQESMNIQVNLNDQIVLLKQNCNSLLNDFEKQQFEISKLQKDKQYFQTQFENQQIENMELMKQQQLLNENHQIQLDSLNNQWQQQNYALQEKLKINESKLNKLYDNSNTLQKQKQELENLLKQSQNETNKFKDQIDQIQQFQIKINKQNQTIQNLIQQNQSYENMINKNASEKKILEQQIVELKQISQNKQVLERNIINEDKQSEIKTLQIENSELTKQIKQEQLNQQQLKTQIVNLQNDIILYQQQKTQIEQQLDKQQKNNQELLKQMEEVIQNNLNQQDQQANEWQNQFNILSKKYNQSDQNLQNALQENKELGERVLELEQKLEEEIDRNQQLIEETKKYQIDSKQKDVQICQFKSQIQILNQQLNNEKSEQKVNQQNESSTGFAQVIKEYEIQTKHLEEEQEKWQLQQNAYIQEIEQQQLKIQQYEYESKENQNFQRQLNEKLQETLKKLEQIQQQLHDEQLKSQLLMKQLSSISENKSSSQPNQDLMDLFDVQQSPRGQQIGTVQLPETNQSEEQISILSQQVQLLQQQIKQQNESLEEKDVMINEINEQKSQLENSQNKSIEKINELENEILNLKQELQNLDQLNKDINHYQQKMSNQQKIIESLQNQVEELQIELQEQESKVKMLGTIQQSNEEQLIFQELISQKDQEILNLQEELKNLQKKVEQRMNKSVISNGSKRSQNIVKFEQFADLDEHDVSVESNEEDLIIEKLQLQKVIKDNNDKIIQLELQLQNLNLHYSKELQQKEKDIIDLQEQLNKFKEQYQDNENTEIDIQKQQNEILDLQKEIEIQNQMIQQLIDDKKLLEEIIQTQQNKNESIKEQSQFNEINDWFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NC85</accession>
    <name>A0A8S1NC85_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0780051</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NC85"/>
    <dbReference type="OMA" id="IVCEQKN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34451">
        <property type="entry name" value="PHD FINGER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34451:SF7">
        <property type="entry name" value="PHD FINGER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="172"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8087143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="361" mass="42271" checksum="027FBF5985FB6625" modified="2022-10-12" version="1">MSQGMNNNSLIPLVGNAQDLELYQMLFSLQQHQLVKAFQNSDLQQLCQTSLLPNLTQSLNLNNSSKDAFPTPNMLNMSFAKTMELIQCFSNPTHLLQQANFLNPYPLQQTNFNQKQKNSADNPITVEDDEPVQQHKKCHNNQCKNVGDKKIKSKRNDILFFCDKCSKLYNKGNYCDFCEQVYGSYDDEAGWIQCDSCKKWNHIVCEQKNRNQNIQIEFETSQYHCLTCSKNIKKPKVPKKIEEPPILKQRPIIEQEDLRNREKNITFVATKDNKIQYTFRFNLMDDEIKQDLDLLRNSTKKKLKNQQSSPPIIQQVISTQQNTQSQQQQIQQETQDSSFVNRNLRRRINQKINYRDLIGEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCC3</accession>
    <name>A0A8S1NCC3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0720014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCC3"/>
    <dbReference type="OMA" id="NHAQIAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046455">
        <property type="entry name" value="Sec7/BIG1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF388">
        <property type="entry name" value="GOLGI-SPECIFIC BREFELDIN A-RESISTANCE GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20252">
        <property type="entry name" value="BIG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="1">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="973" mass="114293" checksum="88F475353C7D1BCC" modified="2022-10-12" version="1">MQKFSLNWKEGLEYLINQGILEENNHAQIAQFLHDNPFNKEQLGQFFGSSKENHQLIFQIYSQSIDFKGQHIVDALRKYLNYFTLPGEAQQVDRCMLVFSQKFFQDNPGDVFKTSDETYVFSYLLIILQTDIYNKSVKTKMTFQQFCKSSKLSMERDLGEEYLRYCYDQILQEPLAIHSSIEKQQNSQINWMNLERKSLQQKIYIFMPRIDYIKLFMEVFWPALFVNLNVTIERTENVQIISIAMQNATYTLQLMSMVGICDLCQQFIQWLCQLASLENKQLKQKNYKALQCIIDLAIKNGNALKNNWRPVLEIISSINYLLNEKQKGKILQEPLESISKNIQNIIDISSIDKVMQNTSNMDSRTILDFLQSLIDVSLNEITLPEPRIFSLQRLVEVTSFNMDRIRLIWMQIWNLLKAHFVTAGIHANSDISLYACDQLKQMSVKFIQQYEHNNFKFQMEFLQPFELIYAQTQFSEVKEFILSCMRMLAHMCYYKLKSGWRVVFKIINQSLQESIVLVNISIDVLNKVFSEDLINLKDIFDEIDQTFQFLFNREEQHVLLKGIEYVQNAVDSLMKHDNNLYYAFWMQSLAHICNLFQNNQQQIQEQAIQSFFKILKSNTQNIKGHQYVQIMKGMMKELFVSLSKAKKEICQMAHKSLLELVFDHDIKEAYNEISTILLNSTFQTNEYLAKTSVMTFKSIIIKEGQKLDWNQTIQILDLMVQNTTPNVLFEAAQLDQSIIDDLLSLNLPKNQIRLNSDQLTLKCILQLMLIDIIQDIVDHHIKLLNEQTYQYLLNIAQNLFKMSFRFNRQIKLRYFLQQQGFMPNLQQLPGLIKQEKSSAIGILNLLHKQSLVNEVLLKDLIQFILEILEDTKLLGQIQVNNQFKGELEKIIINNLSIIQMSVIPILDNLDLKKIQDKIKSLLSYFVQLPSCYSLLVSQCPHCQTCPYKCMQKSEWKKLFEQIQQLQLKLISQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDM3</accession>
    <name>A0A8S1NDM3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0870173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDM3"/>
    <dbReference type="OMA" id="KATDQFH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050817">
        <property type="entry name" value="DjlA_DnaK_co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24074">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DJLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="3"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="126" mass="15337" checksum="83C14090041E2256" modified="2022-10-12" version="1">MKNHYDTLGLERNAQPNQIKKAYHKLALQCHPDKNSDFKATDQFHQINEAYNTLSKEESKSKYDKRLQTRENLNDLLNYIKQQQEEPFLKYDIRDDFISKEDREFLRKFSNKQDIETIKKKRIRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEM1</accession>
    <name>A0A8S1NEM1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0820085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEM1"/>
    <dbReference type="OMA" id="WHKEWQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="54820" checksum="ADD9878BC82384FD" modified="2022-10-12" version="1">MSSIETQQELCKFFILVVNGMRRLEKCLEFIQEITDNKYIPPFFKNSVCRIEQLQVYLQSYGIDTQIYELKDLIGRLGGFEDRLLFSQFQDRYLRQSGNIEKKEAEISFLRLLYEEIQLERQLDDQRVRIFKRWDYNLDILYNKLSSGSNSVDFNKMKKFFVRIRQNLIDEEIKILLKRVGNGDGTIMSLDDFRQALKTQKQRAMDKIQIPMMKTSIVFNKQINNTSYMDRLRRRYKPLQTTQSFIQKDGCQTPRAKTPTPCHNTELKQTRTKTPNQLTEQKVYINESKEINTLMSIYLNQQRCLQNVLMQLDEVTLLKSLNSIDWHKEWQTFSVNSQGITYEHFINILTYYDQRKPFKDKINIIDLPTSALTVADNLFQLLRDQENQLQKSRLQYSKQDQINSLDKYFVKGYMDVLDILQILQKCTITNVSLQDAELIIARFNQFNGNKQITKEVFLNTIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGS1</accession>
    <name>A0A8S1NGS1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0900105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGS1"/>
    <dbReference type="OMA" id="DHRQPYD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="127" mass="14943" checksum="C914E55AF13B1755" modified="2022-10-12" version="1">MSESSNFFVGKYNFSRRVINHRVPIDFNHGITQNPFDFVWRSSFRNLLVSNLIRNMSMWYCGAAFVIGIAINEFQNYRRQGHAFTTPKTQPYLNYVTNSRNKDANLGRWNGNFACWENEPLCGRDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGV8</accession>
    <name>A0A8S1NGV8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0890057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGV8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8091780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="81" mass="9640" checksum="F1BCF976B45E141F" modified="2022-10-12" version="1">MGVCSSKKHHYPLQRSQPQRNIEIDFVKLLEYNRKKEVKDNQNNQQQQSFCQIFLQENKNSNPQTFTQNLAPTAQLDYKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJ17</accession>
    <name>A0A8S1NJ17_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0880002</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJ17"/>
    <dbReference type="OMA" id="FLANNRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002477">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF98">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="6"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8091309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="473" mass="55393" checksum="6A474F929793C63E" modified="2022-10-12" version="1">MQLQQLEKTQKLGQNKYLCIEKKTKKEYILTLQASSDYDDRICSIDHKNFLVILDKWQENGMMCMIQNSEPLQTIKGGGCFGEQKILSIILQLALALNNAELKGIQATVTLQNLFLDEYKNIKIDLLSLNATPLNFASPEVLRKESNHSGIWSLGIIGYFLANNRMPFNGDNDKILAYNIIYKDPHPTDQSKFPILSQIIKWILVKNIQKRYTYQQLINYLNDKVQFRDTPSSLLMNQQFSRTISKKEQSICSFKESKIEIINESDSERNNQSQFLEIKPIVPQIITLDTVENMHSVHRQLKNRPQSSVQRIIKDDQKDLMIRLQRIRPNSAVGFSGKEYISRNIKQELSNRRIIKSSVEPQITTNTFRNNFMKKQQFKEVSVDLKEAKQIKNEHVIRVYNLEYQNEQQIVSKRIHKPFQKILQNPNTPKCLNQKEKVFFDEIQDWKSKMQQHMKQQQKVKKSEPVLKLISQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJD3</accession>
    <name>A0A8S1NJD3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0880073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJD3"/>
    <dbReference type="OMA" id="KEFRCHD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="280" mass="33148" checksum="6B3ED36F57147012" modified="2022-10-12" version="1">MNPKQLSKVYRNDLENSFIRKFQRSFLNLGLKSSQTTRVQIYKQKQPRYQINEKLKQLSEWNEQKYNHKKLIYYPRAKSSESSIEGQNSKKASQFFLLLPYHCSAQKRFYQCRMMWKRLILAVEVILYYSKLQKEQLISKTASCPHIPPKRNSLKSLNYPTSPPKRKFRSRTQGDGKNQLNGFGDYIKAMYESEKQKRELCSKSVIIKEFRCHDSRIKRLGTSKIYDQTLDLSKNGCASIKDPNQLPPLISIIAPYKDENIRIKRVSSTMIRRIRTLRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKT1</accession>
    <name>A0A8S1NKT1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0940097</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKT1"/>
    <dbReference type="OMA" id="HEQFRRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051938">
        <property type="entry name" value="Apopto_cytoskel_mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="19"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8093867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="253" mass="31285" checksum="70F002BE089D5A0F" modified="2022-10-12" version="1">MKNKIKFFFSQLKFNPNKNYYQILNLTNKSTQEEIKTAYYTLAKLYHPDKNPDQIDKFKAINEAYEVLKNPTTKSEYDESQVNPQQYQKKQYDRQYHQHSTYSYQQNVNHEQFRRMYEEYKRQQSHYEYQYHSKTSYAKDDPFKQYKKYTQEEQFELYSSRAAAMRYLIIFMMGMAGYVFFELFGLLMENRQIVVVDQNTGQQYVTTKEEFRRIQQKTREQMDYERRRAILKQIQDEELRMLRQNQQQTEFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPL8</accession>
    <name>A0A8S1NPL8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0880123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPL8"/>
    <dbReference type="OMA" id="YMIHLRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8091543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="447" mass="52156" checksum="E5B94B1A55B35527" modified="2022-10-12" version="1">MMKVISRMSSSILKKQDDMILFAYDNQVKQGEVLKFGKSLGVVMSIFEKNQLALLLQKSEVLEPIQHINSLKAYPQNNVCTIADIKESNENNTDSKLLPKRVLPQNQILTHNVYIDLFHPITYGQMLIIKDKELLKKLNAFDQIIYYGKSPIQTATWFKHAESQGSNYFLPKYALEYANQIARSNKTLLVMDQIYDHFVAHQKLQETIQSYLYEKTTKTVYLSNLLKDLSQSCGYRPGHSNLTVAVLQKDSRDDLLNDELEKELDSLQSHTPSATLAKPSPLSVQIQSPLYYHLAQELYDILSQYNQKKSEYMQKQQLKIHIDPWDFYEYFDCKPIVQMINLFNQQNSQEHELAILVDFSVKAFKQELICQLKGSPKEALNDLLAFSCSIKEFDKKTFAEFYYEKKNELDGNQFKSLIIESLGRFYELYMIHLRMTDQLLQYKKDYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NR33</accession>
    <name>A0A8S1NR33_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pyruvate dehydrogenase E1 component subunit beta</fullName>
            <ecNumber evidence="4">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0910062</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NR33"/>
    <dbReference type="OMA" id="HVQLYNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027110">
        <property type="entry name" value="PDHB_mito-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006667">
        <property type="entry name" value="PRK09212.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008854">
        <property type="entry name" value="PRK11892.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624">
        <property type="entry name" value="DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624:SF96">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="5">
        <location>
            <begin position="15"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8092656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="340" mass="37578" checksum="E985C812BA5BEB5E" modified="2022-10-12" version="1">MLRIVHKLRCGFAKMTVREAINSAMAQEIERDQNVFLIGEEVGQYQGAYKVSKGLYDRFGKSRIWDTPITEAGFTGLSVGAAMYGLKPIVEFMTFNFAMQAIDHVINSAAKLHYMSAGGLKTSIVFRGINGAAASVAAQHSQCFAAWYSQVPGLIVLSPYDCDDARGLLKAAVRNPNPVVFLENEIMYNEAFEVPDNVMDKDYLIPIGKAKIMREGKDVTIVTFSKMVKFSMLAAAELEREGISCEVINLRTLKPLDRNTVIESIKKTHRVVTVEEGWGQCGIGAEICSIINETNAFFHLDAPVVRVTGADIPTPYAFNLEELSFPKTHNIVEAVKLVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NSF6</accession>
    <name>A0A8S1NSF6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1000146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NSF6"/>
    <dbReference type="OMA" id="NPEMNKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="77" mass="8467" checksum="2D4A8978EBF2A7C8" modified="2022-10-12" version="1">MNNIQGYGLDKPSVRLHQAPGGNSSISFGDYTPQQQVPQPLGRKQVNQPPVQQQQQDQKTSVKVRNPPGGRSQIQFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWW9</accession>
    <name>A0A8S1NWW9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0970039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWW9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035892951" evidence="1">
        <location>
            <begin position="19"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="25307" checksum="10F99927030CB41E" modified="2022-10-12" version="1">MDIFLYFIIVAGILQSEAIIINQPGNSIQNQIVEATQYACKQGYFFNGQICSECRSIENGFCTCQKYSECDQISCGPGYYLVGSSCVQHPDSNCLKGSLSSDGSGICIANNNCQQFDSLTGICKQCQSGYFISVGLGYEDSQTVSSTYYQSVNQCKQCQSNCLQCQNEQSCTVCKSGYFVQQNQCYQCVNYLSHCLTCDTASKCTSCDGEIVHYRQTIVQPVHLINIIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NY32</accession>
    <name>A0A8S1NY32_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0980109</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NY32"/>
    <dbReference type="OMA" id="ACIFFIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000001">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000003">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="4">
        <location>
            <begin position="15"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="235"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="508"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8095311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="776" mass="89301" checksum="6FAE99D84AFDBD83" modified="2022-10-12" version="1">MYSSNAKIDTKTQNRLIVCESNANDNSVIQLSQDKLNELKLFNGDIVLLEGKNKKQTVAIAIKNKQDNEKALINSVIRKNLGIKIGDYIFIYPTSSVPNLSKVHILPFQDQISGINERNIVQTHLVPYFKDAYRPVKKGDCFVINMVQEIEFKIIATEPEDIGVVGPKTIIYSEGGSIKREIENKEQYDTQGEDNNIINGYESIGGMNKQFAIIKTIVELQLKNPQILKASGLQTINGLLISGVSGSGKTSIVKAIAIETGACIFFINGFEIISKKPEEAEEIIKKVFELAEKNKPAIVLIEDIDCIAIKKEDIKCQMDRRLFSQLITIMDHFKGIEKLIVIGTTNRPDCIDPTLKRFDRFDKEIELGIPNENERMEILKIHTKKMKLAQDIDLIQIVKETKGFVGADIAALCKQSVLQCLSDKMDYLNIDDQQLDDRTKEIITVTNDNFKVALKTMKLSQFNTNSIEIPKLRWKDIGGLHNIKQQLQEIITLRRDHSKSLQQFVLKFSQNIILYGPPGCKKKSLAKALAGENSMNLIQIKQPLTSLYFKEIFNIAKYQQPCILLYDEFDQLFQKQRNPDAQDNNLIQQFFSQLDDINYEDNLFFIGISNKLDNFEEYIRKNDRFDQIIYVGLPDLKTRISEFKINLRNTPVTQNVDLVFLAQLTNMYSCYDIKNICQNAKIAAFKEMIDIQNKKKITPQQNQLQINSFPQITRKHFDISLMQTKRSLSIELIKQFLEFQNSIEQQRKDNQEKFKYKWPETSQQTFQMELNEEDYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P2V7</accession>
    <name>A0A8S1P2V7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1030171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P2V7"/>
    <dbReference type="OMA" id="SEEQWTH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="116" mass="14162" checksum="9771BB4482C540C3" modified="2022-10-12" version="1">MEIIFIFIYFTVVTLFIIWKSFEEKQSEEQWTHTLRQIHHSLQELALTYENKQHSHMLDHIKENMKILSQMQEQFLNTLLQTQNSMIKKINSCRLSSRSLNYSYRELSFDNELKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P3P2</accession>
    <name>A0A8S1P3P2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1040118</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P3P2"/>
    <dbReference type="OMA" id="KVINLIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097729">
        <property type="term" value="C:9+2 motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10676">
        <property type="entry name" value="DYNEIN HEAVY CHAIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="56"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="2">
        <location>
            <begin position="114"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8097590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="32384" checksum="A0E1DD792A243253" modified="2022-10-12" version="1">MLQTLQNELNTLQTKGKQQELRIENLYINFNEIYQLQNNLNNPTSTKHNMLNKVRPEVFHQILIFLDTKQLLQFRLISSRANQVVKLMFPKQINIVQQLINDQQNELQLKIQSAEEIAKDQNYQQLFNTALNGLQQLNKAHIVELKSLARPTELIEKIMNLICMILDPSFKIQKDSWKECQKYLGRQNFLELILTLDINNINDKQFQQLQQVDSIEQQQAAAQSIAASSMLNFLKAVLEVRQSKLYITQNAIKQLESKIKKEKQLIQKLEKIIQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P4J0</accession>
    <name>A0A8S1P4J0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1050113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P4J0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8097942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="394" mass="46537" checksum="C27903423EA3C258" modified="2022-10-12" version="1">MIIDDKIQKILTSNDFQRVSDKQKLSRSEFTDQSNKSNVTNELKPKKRRVVKLKNQLLSSNVSPDIQKLYYQSKKIDTEQQHTGDKIMIEEQKPIILPSLQKKEQVISEQSSKTNLSDLIMNIQEIFPPDHQLWRDLQVGDQSIEDVIIQNQDYFQDLLILYLQEMEIRSLKNKKLGCKSKYSTQYTDDYNDKFMKDSTYINRWIYERAKQSASLLQNSSTYNQHFVPQRFEKSDNFKPDHIPNQNTMSCLSSYTANYRDWKKQYHGIIGPFYVQSDKKLPFIAETNYSRNFVTQKYEKQDPIIPKPLGPFPVESQSLVRQTTHKNQFSLPQNWIENLPLTSPRNLEIKSFDGYHPHFNKQQQSPQVMNSFADRFFHSSVVQQLMKKNQKQIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P504</accession>
    <name>A0A8S1P504_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1060058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P504"/>
    <dbReference type="OMA" id="DPEQQCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="2">
        <location>
            <begin position="632"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="569"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="569"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8098117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="983" mass="116886" checksum="2D75FA9EF6BB21E6" modified="2022-10-12" version="1">MNQIFYPLTQVQRTSTRQINKVIYYSNPLPEIKLKSNDLKQSIYDWLRQLNQEQLEEVLSFTSYFRVYQYLKMFKIDQNGMHEYQELLCSPFEVNIAQELNKYYSIQNCKRLNKIDLNAHKYILDHIYVSDYEWLFDTIILKGDVEQIINSFETISNGTIFTSCGELESHEDEGYFEINLKKQKVYQTLSELLVDEFQIAILLKYHQSCERNNYTYQKNLLNLNNVRFNYLSLNLNQAYLEFKQLFTHQSNQQQLITEFNLIQSWNEHIKQNSLQFKTLCQEIQYYFNQPKELFSVPLKQILNEFVLFCKFLISRTLLKEFPLNQVPEIIRVQHKQDKQTYHQLQQLSNLFKNSSKSKILPQNDQRLFSTTNEEIENHFYEFTNSFIKKLMDETFIELENQKKFKKQKQKKVKSQKKQYMSECTSNCTEYQQLQQQQEIQFGDEEWINDTQSKNQKKKQRRKENQEKKKFKKLPEIQQVQSNQIIETQQFETSQKIENQKQVEIQEQVQETFLCTTTSTHDDDCEFIEVTKKKFHMKKKKQNNKKDKELKEVQVEKQVILIKKSNSQNYLSDDQKMENQTPKKTLSQKKEQENTSQKPGLVKSLSRDQKTYKYDMDKDTLNNIYQSILEHKISRDIQSIYQKEQDNLLKFRTARDVAILRLQHIIKAHFFQFQVEPVIFGSSRTGLALHDSDVDMVVFGLPVFTKVQLFEPMRKLLEVFSQMKWAISYKHIFQASIPLIKITIDPSIGFLEFLGNPIYYVMNHRNIEQLNLKFGEQSQNIQIDITFELQQPYPMYGYQGYHIGLLSTNYQRQVGISVKGFTEVAVVLKKILKQKGLNDSYTGGVSSFCLTIMLAAIGENISMGDKLLQFLLRYGWNFDPEQQCISLNSEETFMPIYEQGDKLPLNIISPINDEKIQIYVSKIQEILQVFKELYLELKENMNQIAISLQKKLDQKELLQLYQTNIEKLVDLIQWQNTNLLDSQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P9A5</accession>
    <name>A0A8S1P9A5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD-40 repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1090177</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P9A5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="187"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="229"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="271"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="313"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="355"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="397"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="439"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="481"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="523"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="565"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="607"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="649"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8099484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="800" mass="88428" checksum="B5582F31DD3BA746" modified="2022-10-12" version="1">MFRKENISDCQKSLSKDDTQKHIQQYIIEELNYSFVDKQQKLKDVTNNITKITNILKSIYEHDFNKNDYSTKIYLESRQELIQNISKNKKIIQFLKFLVSLTSIDEKFILSGSNSLNLLVEMKVDLSKQSFINIRIKNISIVGANFIRSNLSGSEFDNVDISGINLNGALLLNCKWKNIRINELHHLNGHSSYVKSVCFSPNGKILASGSNDNSIRLWDFKTGQQISQLDGHSQGVLQLCFSPDGILLASSSDDKSICLWNVKTGQQKIKFEGNCNTIYSICFSPDGTTLASGSDDQSICLWDVKTGQLKHKLEGNSFGVLSVCFSPDGKTLASGSNDNQIRLWNVKTGQQISQLDVHSQGVLSLSFSPDGISLASDSVDKSIYLQDVLAEQQKSQLIGHGDSVNSVCFSPDGSTLASGSLDKSIYLWDVLTGQYNTQLIGHGDSVNSVCHSPDGTTLASSSGDQVICLWDVKTGQQKSIFMGHGDSVNSVCFSPDSNTLASGSSDKSICLWDVKTGKLKLELYGHISGVLSVCFSPEGNTLASASFDKYICLWDVKTGQLKSKLIGHGESVNQVCFSPDGITLATGRYDNSIRLWNVKTRKKHSKFDGHSHGVLSVCFSPDGTTLASGSVDNSIRLWDIKTGQQKSKLEGHRNTVRSVCFSPDSTKLVSGSQDNSIRLWDVQNLQHQTRMNGQSFFIEPPFILSEAALSAQDINSMNINVGQQISFDQIYKDISSTFSQRVDKYITILLISQQPIFQAKGALILKGEFINQAGIDLRQLLKQRGSYILESQIEFKQKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCK5</accession>
    <name>A0A8S1PCK5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1140034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8100950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCK5"/>
    <dbReference type="OMA" id="IFTQKEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="418" mass="50077" checksum="30BBF14FE30EA130" modified="2022-10-12" version="1">MYIDPADTKAWAEELKEYQGNRKQKVEFQKPKIFTQKEMKLKQTEFNPVLQTYQNPERDVKEKDKDFQKTSTLAQKKMEMVKKYMHEYDLVNLEQIQGIERLDEREKTKQCLLPPNFNDYNIITNEKKSEEYYKNMKVVVQKRVHEKPVINRNKRDFNIITNKFLDNHELKAQEQEVAIMSDINDKCKRVRNYDIVAGNFYNDEKEQEYQQKLKQDQQNHGKHFNDRFPPSWKFRESVYLDQTKEIPEEIKMIDEINRNHKKRYEIRHVLENEYKERDIQEQMRTQDRLIKRHKYDDLIKKYDKEFDIITLEKPEIDHIVKALPPKPPLNSWEKVQMTKSVSDAPIHEVSIPEGIQFKDNVQNQNESQVRVRFPQVNRQGSEKSQKSVSKSNKSGPKSIVQSQKSNIQGTEIKSGGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCP8</accession>
    <name>A0A8S1PCP8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1130113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCP8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8100815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="163" mass="19440" checksum="50A701A3412274FB" modified="2022-10-12" version="1">MLKKQKQFIENPIHDLSVCPTNKLTLSSLDKPLFPTPWRSYQHYNIQTPTILINQTDRVQEMSFQIDKLKPRYSRSLTKNRKQYLQETAQNVYDPKPMEKQILNGSRLKFNGDLSMNLYQEEPKQQLLLPLMLDLYFLHSFQNRICQGWFNHYQQRCGSTQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PF09</accession>
    <name>A0A8S1PF09_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1160107</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PF09"/>
    <dbReference type="OMA" id="ARRIFWQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="158"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8101736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="469" mass="53509" checksum="F85B53243AA15C2E" modified="2022-10-12" version="1">MSQLKKLNQQVKQVKCITLSANQSHHSHQSSVKTLPKPIASRPVTSITKNNNKVEAVKIDLVKQKKQITNISVTRSTSSARTRSASNGPKEEKIPRILGDLLGPEVELADCQDFLLQLNEPQLKMDEDLMKERVILANRIANTIKMKKRIPTTTADYYKLCSVIGKGAFAKVCLGIQILTGVKVAMKIIEKSSLKNESAKKRLMQEITLMKLLQPYKCCIRLYEVFETKRQIYLIMEYVEGGDLIKFTKEKPLSEQMAKNIFGQLILALQILQNHNILHRDIKLDNILLQGEQIKLCDFGVSRQIVKGQKILEQCGTPAYLAPEIMTQKTGYEGFASDIWSSGVLLYILLVGKTPFKGNNMNELNQQIQSGLLNFMEIKKANLSNEAVDLMKSILNINPKLRFTLQEIMKHIWMKDIDFKQIKQQTNSNLDLRIICQIEQYGYQREVIIKTLQNKTLSHISALYWALYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PFG8</accession>
    <name>A0A8S1PFG8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1170019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8101907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PFG8"/>
    <dbReference type="OMA" id="DNICLED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="339" mass="39918" checksum="E398AE9DF873FF84" modified="2022-10-12" version="1">MNQLEKTPNQRLQRTQSQQDEKWFKQLHRISSSLKNLKTTQERNVYIKKVARQRELDKIIHEKQVIQQKSYLLDRSKKKFLSLIRCKDPETFFLKFKPIQKPLNMLRMVSELNLAEKVQMNLNQKSKHEDESSPIVNRTFNKNNKMGGTKVTELNVSKESSQGDIMKPRKTLEYLFQDNKKQNPLLQNEYDLEKSKSKKQSQSRSYSQYSKSNMNTLLKTDINTLLTEMDQVNTKMNNKLLKISKVSSQILARNGIFLQTKNIPNSIKVNSQDNICLEDDSNILYILDSSLASHTELMLDNLYGNTKKLNSDTKNLEMRKKEYEQESIKLFYKKIYCKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJ07</accession>
    <name>A0A8S1PJ07_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor 38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1190043</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PRP38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJ07"/>
    <dbReference type="OMA" id="ERSHKHD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005037">
        <property type="entry name" value="PRP38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23142">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03371">
        <property type="entry name" value="PRP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="345"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="403"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="445"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="489"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="537"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8102713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="547" mass="64246" checksum="DDD741E5037F4B94" modified="2022-10-12" version="1">MSKNDKNQKLTAYESYKKIESEIKFQTNLLEKSNPTIRCPIHQYNYSKTCRQCKKIVVEEVPTYQEKSINMDKYHYDVGDRGDNIIPIRGENAISNMNSLVRQNILTCQYYKELLQIRDINDIVTETDKIVTSVGTWAGPGVPSSFFCILHKLMSMNLNVKQLQILCDWKLNPYVRCLGLLYLRYSLDPNFLWGWMKRYILDEQEFKPSKDETITIGDYCERLLTDLNYYNTRLPRIPQQIDTIIQAKILLAQEKRQRRNINQRIMPLLQPGVSVRAISQKDDEWHVGKIENIDGTFLIIQFEEEKERISLGEIEFIPKNDEQRLYIAQMITEGMQEGQIQLPQEVISQKSPSKSKSKKKKRSSSTSSESSSSRSKSRHRSHKKKKNKHKSQKEHKKKKDKKKEKEREKLKQKKDKDREREKDKERDKEKERQRSNSPELGQYETNKERQERLEKEIRRKQMDKAVAHSKQDVAKIPASYKTSLAAALPTSTKPVRQSSPSPIKKKEQFIELGNSDNNNTKFEKESKTDQNNPFLKELIKKYGDDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PNC5</accession>
    <name>A0A8S1PNC5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1240121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PNC5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="127" mass="15410" checksum="E1B5C7279F5BF6AE" modified="2022-10-12" version="1">MMQHQNFQLDQLMIRNLTQYLLITRQNQKKKGKLYKFLLYNTIDIWYIKSSKQQNFFSKKKFNYQNPCIGCLQLTNIIKRKRFPIKSVIKLSNKWNHIVNKKKGDIMSIKLTIPKEYSNNMPILFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PS75</accession>
    <name>A0A8S1PS75_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1270110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PS75"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="150" mass="18381" checksum="9D30CDC1FDCBABC2" modified="2022-10-12" version="1">MIQQEKVYKNTIQFIEVMEIYKTTNAYIQYIQRKSEYDQCIENRENQKFNKTIFGIIQNLVLEDNDESQLKTFFILSQFQVWFNLLNQINQFLELEQNIQQNCDGILKIIKFLLLKTITIELIIVQFHRVNNQKAKFYFYYPNNIKYFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSP9</accession>
    <name>A0A8S1PSP9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1290069</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSP9"/>
    <dbReference type="OMA" id="DTFLYYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000121">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="353"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="146"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="101"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8106042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="554" mass="64220" checksum="91052E80319446EE" modified="2022-10-12" version="1">MQRVPQYVQRPVVQDSFRQSFKQTPDKENSYAQLQKRKPIQAYPQYQSTQTLQKPEMRPNSSQPRQPSVQVVPRPSSASNDKRAFSNQKQPSRCSFKELKNSTYIQPQQATPQSRYYSLKQVPDTTLVKSQSNQQLPNKQIDQSEYKKLHEKLLFLENKINKIKSNIEISNSQLQKQSERAKQKPVGLAAKFFQKKDQENNEPKESSTPKELTKCATALNLQLNKALKQSQIGKEKQTSVNLDQFVTQVKQLKKPIQPQQSQLSRHQSLEISQTVIPSTKTVINEKSYSSQKPKKDDTFLYYISSVIRVLVQEQITKNDEVVRDHIVQTIQGLEYARNLQLQFQEDKVMNLPKSNHFKTLVFDLDETLIHCNESVAIPGDVVLPITFPTGETIQASINIRPFAQQILQTLSRHFEIIVFTASHSCYANVVLDYLDPKKQWISHRLFRDHCLQTEDGAYIKDLRVLGNRKLNNILLIDNASYSFGQQIDNGVPIIAYYDNKSDQELLYLQNYLMKFRMVTDVRELNSQLLKMNSYTNYQDPLHLVQDLFPDYLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PU62</accession>
    <name>A0A8S1PU62_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aminopeptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1320041</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8106890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PU62"/>
    <dbReference type="OMA" id="GENCIMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000006">
        <property type="entry name" value="Puromycin-sensitive aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF299">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="10">
        <location>
            <begin position="37"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="9">
        <location>
            <begin position="247"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8106890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="841" mass="99266" checksum="B7A378D0CE7A8E0C" modified="2022-10-12" version="1">MLQEQNGIRNDEKQIKYALLSKDQAEQRSMDIENCQYTLDLVVENDIIMGMILINFTCYNDHIKLDFCGQQIQSVKVNNNEWSLDEIVKNWKKHQLSINTIKGENCIMIAFSIQFSESEFGLIKYTQNQSTYIISLFCPNYCHSCFPCFDQPDIKAKIKLQLICPKEWLAISNMNPNLIEQCSETQNQWNFATTPKISLYLFSLNMGQWKKITKVLNQGIQMNLYTEQDKYITCLNSSILIQKCMEEGFNYYQSLFNIPFPFEKYDLIFCTFYFSGMEHPGAVLISRNMIQNRFDSNQLTKLFLLLLHELSHMWFGNLVTMKWWNDLWLNEAFAVYISYEALASVQQQESLKGYKFNDTKIHYLLYKQNGVNLDINSHSHPIEMKIEDANQGLQAFDSITYNKGSAVLSALVKLIGFNEFIEIVKQYLDKFKWRNATTNDLFDLIQQYSKVVDIDRWRREFIQQNGINVIEIITQDQQSILKQSSISGNAIRQHYMNILLIKENNQMEQKQIMMTKECHYLCESKDYSAAILNYTDDAYCISTMDDKSLSFILSNFIELKLSNQIRVLILNNIFQMTYILGTFKVKYFLEFVLQSMNQDIDAEVLNFMIEKVEILFMNLNVEQQLKFGPIIFDKLYLLISLNIELKEIIHNQIGNFAFNQEHIHQIYEILTLHQKSNRSLMLLGRFLDCLFYKKHLLNQDHLQSYELFWSTLKSYNPNFVIRQNAAAFTLEEIQQYFNSLLQNTAEYNQMENMFKGINNPYSLIYDQYLEIYYSNIKNLCACLDQHKILIVLNEGFPRQGDFNIQLQILNELKQEFPNLNFTLNKLKQRSIQRQKIQQSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUK6</accession>
    <name>A0A8S1PUK6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1320099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8107007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PUK6"/>
    <dbReference type="OMA" id="ENWCISI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024453">
        <property type="entry name" value="Peptidase_C92"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47112">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47112:SF1">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05708">
        <property type="entry name" value="Peptidase_C92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="69"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="365" mass="43107" checksum="1CE0DFAECE8D85B5" modified="2022-10-12" version="1">MLEKRLRSISVQNNQNPLIDCYFRSECSPERLKRKSQKSQQTINLTSPIRCIERNNVDDFEIYLDGNQTVKEGWLYQSKRVYVQLTRQSIKEYNDEQKQQLIRVLSLKEFEFIVEQEKQLIKITFNHLKQIILYQAKDVVEAKMWVNSLNIVFDKHRNYPKNRLQLQEVHRYLGVEVISEDYFLKIVESGDLLLFETNNTGAKLQRIFTNTKYDHVAIAIKMANKYLFIFDANADTGVTFLEWSQFIEVNDLYEKLAIRKLMNVNRSEIEKKMIEFLQQVHGKKYEVTLSKLFRQKSLSPSKKNDTYFCSELVAKAYKKCGLLESNKACSSFWPVEFTKPLKFSKESFITDDIVVILKKHLAIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYX2</accession>
    <name>A0A8S1PYX2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1370032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYX2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8108384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="57854" checksum="F438B0D0DC81653A" modified="2022-10-12" version="1">MNKYTLIFRDENTEKKYQHQKKYGLNIIIGGMVISFILRTIMKVINFEWVNTGINLGVVAMLIFLRSYSKYYLLKRLSLIITNCGLSIFTFIYEEPSNNMYSHLRGANSALSSLIIVLSGDFPESVVQIIFIQVTKGIYTILYSSQNEYEILTGNILIMIILIYYLYFHHKAIRSQYLLTLVEQKWENVLESIIENQSYFIINFIQDTYQFQIQSQHNCEQYFYDKDYKTFLRQSQIEKSNLEHYFFQQIQLHLNNHNPINSSIIFVKNSLSLFKLKYSIYFTTQPTILIIFETVQQSKNRNINLLYNKIILYNRMLQMSINQIQNQNFLYQRLKHYLQIENVMFQLEKGNFKLQTIKLNKFLMKFSFIQNLSNYQQKKELKITTLPNVLKALFLLIRDFSIQVKDREKQIQNSIFLSYQDVSENQVVLILQGRFQVKQIQNLIDIYKLQFYTILSNIDIYQDKIIKLILKKRIQIPFINKLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZ16</accession>
    <name>A0A8S1PZ16_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1370083</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1370084</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZ16"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="184"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="124"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8108484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8108486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="246" mass="29278" checksum="06C87EA1CCCC5C73" modified="2022-10-12" version="1">MSDKKNNNIPQNNNQADDSNYALQMQIIAFQDQTINNLKNIIIEKDSELQKKNQENAQLKQQIQMIEKQYSQQQMSYQQENSNILNLQSQYQKTFDELKKLKMMFNQNSNPDIVMESLQIKEQLVIIQQQKNKLEIELKEAQTRLIQSQGIIQELQRENYQLKQFSMSSFSNSPQIQQPSINQSQFLQSQQYPSNSQSQNNEENQRNQRKVQLKNELRQIPSQNRTSAQQRRIQQIEDELNYLEQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q0I7</accession>
    <name>A0A8S1Q0I7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1370013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q0I7"/>
    <dbReference type="OMA" id="NAQITDN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05162">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="7"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="323"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8108348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="482" mass="56259" checksum="9250A8B1264B1934" modified="2022-10-12" version="1">MKSSLKKKQIVWARTKGHPWWPGIITQVFDGNQYAINFIGDDSQQGSILMSDNLFDFQEKFEEFNDKVKKNKKLKEAIQNAQKLQKKQKKKHSDSEQEDKKKSIISKRNLRKQLKKPETPNKDNIQKSPQQETPQMQQKKQYLDREFQDLLLLLIAAQLNYDQIKQKLTPILDTIEAQVAQNSTITEVLSERKGSILNAVYLILKHQSEQNPDHFFQRDIHQQITRLHELISKLKNSLLNQFFNAANIIQNLSEVCFNGNRGQVFTTIMNQDKINEKKIAQVSQTASQKHAIENLQQTKRQKKKEKTEQKIQSNNGNVQIPENTTVQNSNNNNNNTSNQSKKDQITNEESKIEEKKNNTEQTKIIKQDIVVQKKLQKKNEIPDQALRRKVCLNMLELVQLLGMEEEEAKKITIKIESLGREADPLMRNKYAKLMWHCIVNLDSKLLKREELLNLIDENADQSDVWTKLTLQPIDRTRKLIHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q0W7</accession>
    <name>A0A8S1Q0W7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">inositol-1,3,4-trisphosphate 5/6-kinase</fullName>
            <ecNumber evidence="3">2.7.1.159</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1370090</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ITPK1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.159" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q0W7"/>
    <dbReference type="OMA" id="RYTLYDM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052726">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052725">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047325">
        <property type="term" value="F:inositol-3,4,5,6-tetrakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032957">
        <property type="term" value="P:inositol trisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008656">
        <property type="entry name" value="Inositol_tetrakis-P_1-kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040464">
        <property type="entry name" value="InsP(3)kin_ATP-grasp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217:SF1">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05770">
        <property type="entry name" value="Ins134_P3_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp" evidence="10">
        <location>
            <begin position="277"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8108498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="482" mass="57652" checksum="E3544E3ABBA74582" modified="2022-10-12" version="1">MQQNQKPLLIIDSRYTLYDMRQKQVYKYNTVLQKSFLLLQAHFQIQVIFCHSKPNTNDFFYNEFQRNFQGIDSKKLSIQELKEISFQNLELIVVTHNYEQFTLNQNYQLYYFSSISQQLDKKRELELKYYQQNPKPKFHISHILQVMSYFSKSENPFCVGYYFYPKKFNELIQFQTLISNNNFTYLPIDFRFFNKYIQIDLLFHKIMDIYKNKEINMKENEVQLFQQNYDKFIQENSQLPVIDSIECLQTLIQRDELNTKLESILASPEFQNVVKEHHIKIMTPRQIVYNNIDQVQDLSNLEYPLIVKTKQGALTANCHIMAIVVNEKGLKELFKQKQFKGELILQQIINHNSIIYKIYQLGSKVIVQKRKSIPNIEINNFKFEEGFYVFDTQKDLFKNVSECLKQVDEGIHECSNEAQLQKQMELLSSIISKEFKLHLFGFDIIGMNQEFYIIDINHFPGYKNVENAIELFEQLFIQVSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7T2</accession>
    <name>A0A8S1Q7T2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1480053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7T2"/>
    <dbReference type="OMA" id="MKMIQQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="641" mass="76834" checksum="7786725D56F9EC3C" modified="2022-10-12" version="1">MQEIQDLDQKQGNSYQNNQQFKNSQQQITYNIQPKFQQHSELIQYEQPLIQQLVLNKIETLGKTISQVLAIEMFKQFTTKTRGWSLYLLQNEISEQRRNFLNLQEDSLKNGLQEEWQVAREEFKKFLKMLEDLLQNQEHKQLLQNILQPPPALSFIMIQPDMKFFNALENYHYQSKINFHNSLQLDNTGQDLLEKIKTYKNELAKFLYFKFIEQGIRYLDQLLNYVLIHKIQNNQFTLQLLNDYIRQLRTENDKQQLLNEIQLIIIIFPELAQYFQEIYNIINDELLYFDGRVELQEQQFEIVMLIYDYIVTPIRILNRKLSNKTICVMQQLNWIYRTGSVQTKQKQLKDLKKYIKQNHSQELQLDLDYLSDLFISTKLKLLMKMIQQINVLQDSDIKFEYFNFHAFSFQDQDIIQQCLKQEDGHFLNIYHEPSIDEQQINIQQVFNQFNNVIEFAAHCEMDLNKNAQNNSLEELKNKIKDSIQSLANQITSTKTIIKLQALLNQIFWFQKLNGNTFLVLYQYNQEISEIQLKKQDIIGNEEVVPNLYLLLLNFDQEKDKDILFQKKVKEITELISEDYAVDFSDLIKEIEQFDQKNKAAFIKNAKQYYYKDFKVHLLNLLQYGTKQFQEAINYLIQQVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7T9</accession>
    <name>A0A8S1Q7T9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1480058</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7T9"/>
    <dbReference type="OMA" id="WYNTNID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033565">
        <property type="term" value="C:ESCRT-0 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="55"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="308"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="182"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="308"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8111598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="342" mass="40760" checksum="973766BA8EF70F3B" modified="2022-10-12" version="1">MSSNKNIEQSPETQSIRNSIRHQSLYQSVQEFSGYKEKMQDLMLPNVEFIDPSNFKKQNECIVCSIEFTQTNRQHHCRMCGNSCCGVCSQKTINKNRVCDICYMKASQITAEKKRTKFLQTLKESAKKLRKHIEQAEKKKQDLKEERDAQSDKSQIDLRVIEKETEDYRLKYSQKTQDKCQYEQEVEEIKKKILELNSLKEQIQQEFQEKEQEIRAYDTNLYLRDQELNDKKTQLQRLRQQKEELENMSNKIEQNPELVEEISIPEPIQQIQKEQQQQQWYNTNIDIDEIMYGNSDNSKDSTTLIKQELPQKQLKKQNPNTPSQTKENNEDENEDKYKCIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8W1</accession>
    <name>A0A8S1Q8W1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1480002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8W1"/>
    <dbReference type="OMA" id="ARQIQIW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="114" mass="13308" checksum="A405D07AEB35C0B3" modified="2022-10-12" version="1">MFTLSQTDQEYISVFETNNINKQFTKTRDITIKCGSDANCLFPQQYVNSKCILLSKNGEYVNLIRKKQNGEFLTEQSIHLGTNDLFGGMSEDGQYLITWDTQARQIQIWRYHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8Z0</accession>
    <name>A0A8S1Q8Z0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1480018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8Z0"/>
    <dbReference type="OMA" id="STHRINH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="220" mass="25783" checksum="1BB6045C85CF3519" modified="2022-10-12" version="1">MVKLLSKSPVALNSPTLQQWDNSFRENYEMYQSEIDKNTYALQRSMRGFWIFGKTFYPALTQHYSQKLELYQLKQQLGKIEQSEVNLYKTVNPNISTVLNKTFSTQWLWWNLQDISITAGLQVYAQLFNFGYSWRVGFLVLPYCARVFYDYATSYQNKSKAVEFLNWAIQYRKAKSQIERGSLDPQIVERYQALFGSVKKGPLQVYADVVELATQQNPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDF9</accession>
    <name>A0A8S1QDF9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Copine</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1530045</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDF9"/>
    <dbReference type="OMA" id="CWETTEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000147">
        <property type="entry name" value="Copine family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000515">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF106">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="137"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="3">
        <location>
            <begin position="305"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8112885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="553" mass="63291" checksum="F286DA5D0CBF06B9" modified="2022-10-12" version="1">MDILIHQSPGELVYLYISARRLQNLGNSNIQDFRCRVYSKDDFIGETEVSRSTLDPDFKQPIPIMYKFNKRQKLVFQMIDADQELASQLVSQNTSTSIVGICKQPLAKLMGARNSLSQLNLMKGDQIVGNIIIHVARKGPQIIGQKEQGHKVTEIKWRWGGVKLLDLDFFTKSDPFAKFYRINGQQTELIHKTEVIKNNLNPNWMCWETTENELIKYSKNLYVEVMDYDKMGSEEIGHVTINYDEIKINKRTEFPLLTPKGKNAGTLKLLELIIVEPNEEKKEIVQEEPKEPTFLDYLMGGWQMSLSIGIDFTFSNQPITKPDSLHKIDPHKLNYYQQAIKDIGGGIIAFDYDKQVPVYGFGGTPKLPTYTKSTMDDCFPLNGNKDKPFCNDMEGILQAYIEAVPNIVFSGPTFIANVLNKALQFAKDNAQKNTYTVAMILTDGQIEDQDDAIKVLLECQSLPMSIIIIGVGDENFKYMKQFDDPMFLKKHSKSDMNVRDIIQFVSFQDYKNDIEQMSSAVLDHLPKQFMDYMHINNIQPIKIQSVHLSKAFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QHB8</accession>
    <name>A0A8S1QHB8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1620065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8115013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QHB8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8115013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="87" mass="10535" checksum="CA350D68D1610016" modified="2022-10-12" version="1">MSGQKKLLIQFLKKILILNNLIKHSMKYKNLMKANNKNQSSQQNQDEQIVQDQPIQQNQEYKEEQIIQDQKNNEEVEDFIEDYNYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QWS0</accession>
    <name>A0A8S1QWS0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T3220002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000331">
        <property type="protein sequence ID" value="CAD8119435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QWS0"/>
    <dbReference type="OMA" id="TCAYKDA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051266">
        <property type="entry name" value="CLCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579">
        <property type="entry name" value="CALCIUM-ACTIVATED CHLORIDE CHANNEL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579:SF43">
        <property type="entry name" value="ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="171"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8119435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="593" mass="67473" checksum="B4FA5907B3DAE219" modified="2022-10-12" version="1">MNQNQQLQSMFANPSNQQQQQQQIQNQQQQLNFQNFSQIPMQSSPNFPILSQPTHTNYNIFGGPSQQQPQKQFYAQPNLQPSLFGINNMVIQNQLPINFQDDEKVEPKKEDAKQTSNKYDLNEKLSFEVKTLYKMGKLMNSRTQYLPGIVSIKAQDQTITQNPENQRVGVDLICLIDKSGSMDGQKIAMVQQTLSVLLDFLSDCDRYQLITFESSAQRLTPLKRVTDGNKQYFRQIISQIYSGGGTNIGSATDLAFRQLKERKYRNNVTSIFLLSDGQDGNAVQRISEQIKTVNEVFTLHTFGFGQDHDAPMMTQICNLKSGSFYFVQDVTLLDEFFADALGGLISVVGEQLEITITSQAPPPYQNIQISKTYGNMWQKKGNLYYITQPQFASGSRKDYVFELVLPKFEAKIEDFQRNVKVIDAELKIKDPVNGTVITKQTSLTLTFFNQDEQINQNEADLDVYAQYFRVKGTEVIDEARKACEQNKNEDAEKLIDNMLIQIQKIPNVAAQCTGIIQDLQQAKQASARTSYSLFGQKQMCQMVSNNYQQGGVNSLFSAQGMQLQQVQPAAYQNVQQQQMMFQVQYSKAPQKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMF6</accession>
    <name>A0A8S1JMF6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0040098</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMF6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="21"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="436"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="350"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8042881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="495" mass="58585" checksum="391B7F96D9F44AA7" modified="2022-10-12" version="1">MQDENQVNQMEEEGISKCEGYYIIKYLGHGASAVVKLGQKGHEQVAIKLLKVKNEQERPQKIQKLQQEVQILEQLNNHNYDGIVQLKYFSDNLTYIKKNGQIVNKIGMILEFGEKGSMNSYLLTLKRFNEQASRFYFRQLMTALVYMKGLNICHLDLKPDNILFDSDFNLKIADFGFSKQANGLINDFFGGTKNYMAPELWDRIAYDGSKVDVFAAAQILFKMITGCFAFKNCQEQLYILIQQNDYAQFWNIQNNTHQTLFPQGFPDDFKDLINRMIDPDFNRRITPEQCLQHPWTMNGEANLYQIQEQFNNVQERINQQLKQQEEEYQRQQQMRRDQERADQEMRNLLQQSQQSYKSGNSQKKNPMINKKRQLMKKFKFTFENRVLKKSLPTKMIDEILIYDDPKNLFCSIIEHFDKCGKIINIHETKYKLNVNIMENDQEKQEMLIELKKLEGDKLRVEVLNLGDDFIKFNEEIQNLKQSIQQQRDEVQIQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JND8</accession>
    <name>A0A8S1JND8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JND8"/>
    <dbReference type="OMA" id="CDININV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035934347" evidence="1">
        <location>
            <begin position="19"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8042899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="335" mass="38047" checksum="CF74113E59B973BC" modified="2022-10-12" version="1">MNFLIISIIVGLIVVSSSQWQSYHLLYSDVKFTYKNDSSAQMHSGGFLLPSRETTANFIDCNSPSTTYITLNSTYPQTRSSFMITLKNGYLFSVDFYFQGTWTSQTVTIMINQVSYTYNYTSPKNYQSTSGFCDDFAYEVKTVTFTYQQPNQTSGQLYFTSQNTNDGQVSIRNISITLPKCYPSCKQCTGPQFNQCTSCYYGIPINNICLSHPSILQNLKEHICRDICDININVSCQYNGFQKSYLNETCVKNQIKLCAPQNQLQEAEICIPIHEDQIKISDEQYKDQFIQPYQGCQSFKAKCQSSCIQCDNTGCGCQTCKVGYRNIDNVCQSIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPW5</accession>
    <name>A0A8S1JPW5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0050202</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPW5"/>
    <dbReference type="OMA" id="FVIPYEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="349"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="487"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8043549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="829" mass="99166" checksum="0D80E5D7EB185A15" modified="2022-10-12" version="1">MLDQSEAYKIEDKLLITKQVNTIGNAFQSMRAKFYGFRMKNKAIQNKKQYQQLAPIHFHIIKDVSRKEYYQKLVKYGEKLKRKLNRKWREMKKSKIVQKITKSIKLIPIINPNNEFKLIWDIFLAFIRFYLLFVIPYEIAFNNKLLYNQLLWTLQVSIALLIFDILLNFITSFYEKGYLITNHYKVSRNYLRNGFIYDIFCVITLVLVLAQELNEQNNYIDYKHQSLREYNYVRSVISILGIIIQLQNIIRVFQRLQNSQDYSESTSLLIDLIKLVMFLFLLEHLFSCIWYFVGIQEIAEDSWIKANGLSNATYTDQYITAFYFSSVTMFTVGYGDVVPKNIYERLVTIGFMVCSTLQLSYTVSAIWNVYSKLNEKSEDHIRKMRAINTYMKSVNISNQLKYEIREYLTFYWKEQKLEENLEVKDIIGQLSQDLQENLIFEANSIIVKGCKLFNDYFSHEFKAECLKHVQSITLTPCKTVPIDQPCLFFIERGSIGTYLRNNRLRLATLQAGDNFGLREFMLDVIPSLRYTSVAFSKLVFITKKDFMKVLLQYPEDHEQYCQLKEQIIFDKSYFGMDCYSCKSLQHTIQQCPLLNFNPDKDSVIKKHQYPQLQNRRHYKRIFEKKRRAIFKELKQNDNLDKIQLTIPTIESSDSSEFELKESWKASHSNYFQQIQSITRRQFSNRQALNIKQIRQKLHPPNQNIQILELNNPREFDEDCLRERDKVLLEDLKVRLNQRVNLEIDCVKNYKNYYPKFNSAWLFKTTKINLKFFEQWKQLSLFLTFPYEFIKKYRQHILRDNQLVSPQKDVDHHRKKKRATALYFKPVQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQ27</accession>
    <name>A0A8S1JQ27_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0060077</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8043995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQ27"/>
    <dbReference type="OMA" id="CRDDYIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056290">
        <property type="entry name" value="CEPT76/DRC7_peptidase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033551">
        <property type="entry name" value="DRC7/lobo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056292">
        <property type="entry name" value="DRC7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056291">
        <property type="entry name" value="MORN_DRC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35249">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35249:SF2">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24656">
        <property type="entry name" value="CEPT76_peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24671">
        <property type="entry name" value="DRC7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24667">
        <property type="entry name" value="MORN_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CEP76/DRC7 peptidase-like" evidence="5">
        <location>
            <begin position="276"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein regulatory complex subunit 7 MORN" evidence="6">
        <location>
            <begin position="413"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein regulatory complex subunit 7 C-terminal" evidence="7">
        <location>
            <begin position="729"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="636"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="747"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8043995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="830" mass="98944" checksum="0A128D2A6888C0B8" modified="2022-10-12" version="1">MTSSAVSDVEIQKQAQQQQNNILAILKSGNATVVNEKLQRCKDFKEGKDITIPFCYKENSPKEELVLEHVQQFQKQFQLHYDEKRNLFLYPKNECDLYKFICTTIRPQKIGYLELYNYEQCARYMSFFIAYEQLDPPDQFPKVIPSPTNVARWQKGDCFDLSILLCSVLIGVGYDAYCVYGTAPRSITSKNESDLDYIFINNGIKEDDEDKDKDNDELKDNEFAILPKEEIISKYDTKIQKQKEEFEKEQRRIALTIDDDEPDQMGQDFYAGRRIHCWILLKAGKRGVERNLFIEPSTGRIYQINESPFLTVDAVFNNRNFWINMKLESKVADLNFDEMDTSMNWEYVMLDTMVEQAQQDDEDYNYGEDHNQQQKQVDPQQQQLQDIAQILDMPPSWPPKIFIDKDAFLKGTPLGESTVFYKKCKIDSFAPYSQEKDGLVQRFTIYQDYKRLKVQEIRYFYKHRSDKLVLKRRFPYEFKTIEEYEPGNKPQRKTITTIDRQLRIIVYYPTRNHDGLIKRIEKIGEKTIEEYENRDDRVIYRSVRFDPKDKKFEQRDLTHNDRYMGQVKITKMTQKYELSELYPASEQPQKVVIDLQKNIIKVYYHMNKGEINPQFKELKRENMHSLGKLTEPGIEKKNEDALVSQENQRIANLEKECLTHIKNQEDEVKKDEEQLRSQDLKLEKTLHDKARERYKETLKKSEEEKQREQADKDYLYPYLEKRKLQGKEVLSYNEALEIQKDVMTKLKERLLSRAAIIQKKLEEERAKLDQAEQMQQKKSDPDDNEYINIQFRIDILEQRAIRFESQALLKYEEMDRKLKEDKRLSELKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQT1</accession>
    <name>A0A8S1JQT1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080501</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQT1"/>
    <dbReference type="OMA" id="LIVCMYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="106" mass="12212" checksum="318AF807A7F78172" modified="2022-10-12" version="1">MITQSPNFKLNPMTPLSVHHKLALIEDEPDDLELDQEYQKISFNENLIVCMYKPNQNVTNIQKTILKLKGQPNIGWVNPIFKDEKIRSTSILKCSQSPIRICSICQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRI3</accession>
    <name>A0A8S1JRI3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRI3"/>
    <dbReference type="OMA" id="GNKCDYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="68" mass="7926" checksum="03D3C59538B554A0" modified="2022-10-12" version="1">MNKASGATTYKNNQGQKSQKSLQSKLPEKVRLENHGLVIANQKEKRVKKNEIRKYLMSQNKSYLQDLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JS81</accession>
    <name>A0A8S1JS81_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0090096</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JS81"/>
    <dbReference type="OMA" id="ERKTYYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000854">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="108"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="366"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8045071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="430" mass="49789" checksum="D7ABEE4138FE6033" modified="2022-10-12" version="1">MFKKGTNIRDLFAESEKPIQIIVKRRASQDMPPLVSVSPKLIAQPKFSVHVSQAQIHSPERKTYYLGELKTTKRKPLPHSNSITIKNTNLSTTLTTNQYNSYVGKINFTLKFVIGWGGFGKVWKVEHKRSRQIFAMKELQKAKILGKNSVKSVINEKNLLSKLKHPFIVNIVAAFQDRETLYLVMDYLSGGDLRFHLILNKQFKEDQIKFFIGCLILGLEYMHSNQIIHRDLKPENLVFDQSGYLRITDLGIARVHKLDNHNETSGTPGYMAPEVLLRQNHSYGVDYFALGVMTYEMITGKRPYIGRSRKEVRDEILGRQVQLSQKECPNYSPSIIDFTNKLIQRKQSLRLGIGGISQLKDHPWFNGFDWISLYSKKMAAPYLPKCEEYNRKLQQGHQESDKTLIEEFTKQLRIKEVQQQFDGYTYIHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSD4</accession>
    <name>A0A8S1JSD4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HISSWDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035740354" evidence="1">
        <location>
            <begin position="19"/>
            <end position="2187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2187" mass="246588" checksum="E9C2FA249F88FC9A" modified="2022-10-12" version="1">MLIILLLLHLANCGHISSWDIQLDYTNAGYASRFTIPFKLSNPLPDFGYLRIKFPFALHYTNTNNVPDNVVVTFKQAKGNYQCGEENEQSAQVFVNTDENNAYYVKFGVELTSNEYYYVIIQISGKNLNIQSSGVKEPIQLATVTSISAIAMIIDYTPVGGIFALDLEPSSDMRSQVSETLNLMNQIYNVTIGVQFKKQMNEGARVQLIFKSDFNFEGTCSLQNEIPYECTLTSQMVVYLIKQYILVNDWFYFQVSVRNPLRVTLPTSLEIRSLQQYANTINERTVSDAVFQVKQVLLSTHKLYLGWGLEYSSRSASYYPFPIIVFRGDANGKYTPYNAFKWQFAMASDLPEDIELQIEVSTPMKFKSYLMSINHNLPSYPGKQSKCIFSNSNKLSQSKIVCYNIGQLYLSTIYYIGCRATFPYDQFNLPLEQKFGSMNIYTVSNGIIDTLSVIVESNPVEYIQTNNNPDWLNPSYDGTVVSSQKMDISILNDPLTNTLGIIPNLDDGDIELIRLKFNLAIKIDKIITPATTPVLGTQKISYSAGIVIIANDKILMSSTDPATLECATLSGSEFFKYDYKSSNLANFLAITVASTDAQGLLNMFVYNTNELQTKFLQFAISNLQVKFHSSLYPDEYLLDFYIGSFDQIESTYPIFKQSFLTNAYTITSPSPAFIRLGIVNYFSSSSSGNTCDYFPTLIRLSGYFQDTDLENAQTKISLFFTNLQPIYISSDFENDNRVYCKYTGQTQPTCQYYSSGLLANGQSYINYERLDVEFESLPTNQADAFQLLIPVKTFASNTKVSFFLTAVVEINNLIQIRTSFRLTGKDNISSGQYAFPLSVAGLGNGFARAGVTSALESDGITPIPYQLQVSSAITVGKNNTDLTFFIGHNDVSFININTVSNDNQNGGGFTLVSTEPFYTIDSTLKAPISVDSSKVSGCMTFEYLTKFVIFCPTDKASDSPLTTLTIKAGNLPFINGDKLPYRTVYAWSDKVNGLGVFKVDVDTLITLKGVVKILQVSPKLEIGQKQQRIQIKFYTTNPIPNQATIRLEFYELIPTINFDAINKGSRCSIETALYGLLSHTCTVSRSTSGSNVYFLFKITNCDSCPWTNGQFTLNIWGLNSLHSGAVQKSQFHMRIMSEYLNTIDVSKIQGAIEYDDDPNVAYVAFFGNPIFQFYNRRAKGQISVDFQFTRSIWKQEYVLINIGGFFDDNQRNQKNIICQVQQQGQPSYVFGQADYTLDRGSSVSGIKLYPIDDIIGSQIFTLKCYGMVIPFSLNPEPMDASLILQGTFLIIQSSGTISLDSLYAETILPIKNIQLTQKLNNLCGSTTDFFINVTTTLIDVKLGTSIFVVFPTTYPFIVSSNLTYCSLNNLFVACSVDVENTVILENFDISIPRGQEMQIRIYGAQNSAIYNDQANEIFVAFTNNIDITVLGEYGVVYDLIGQDMVTTPPNNILTLWIDTTQQYIRAFSTITFKVSFPAGAIDISNIIQIEFPPAWDMIINYKLPICSLYDQNNLTNYAKSCINFGNRVTITIKQSDYIGSQFYYLILANVRNPDYAVCGINMWTITVLNSTYVFGRSHAPNFNIPIFPFISNPNQVTLKWIDMNTRQSIRTLQVKTGIFDQVIGLASDAGLFLRTFQIYCDNPYFIANPTSAVAPMGQAYTTFTIAGTSKAPQGLQLIEIAKTDDFEKHTQLPFLRVFLSQKSCILNTEMTLYQLTLGGQALPILFNLTDCQPASNLRIEATSDTDKIYFKSKSSITFGLNQKVGAFLFASEDSGISVGDSGLITFQVQGSYQKYYTTVDPISYRIIDINKKRPTSSLFSVDRGSNYVKFIVSCNQYAVVYYYISINGSEILYPEEIQQRTFLWTEPSINDYYHQFFGLKNVFVPLKNYTVVVSGLRPSTTYNITSVCKNVETKFSVATTILQTTKSNNGKSFYIDFIFDSEIDLEFSTSITCFLTQQFKVPARLIRNSINTWCSLQRRRILSTTNDIVYNEKNVRMYVSLIDFELQDTVTASLLTKGINKTQFISDLYDQVAGLPNLVKLSDLTAISTTVPIFQYEVTLNISTNSIALLNISLDQEGFVYCMIDFNNRTKMPTFYDLREGNALRKPENNTYYERQRFNKSSSLNFTFDGLTHYTNYSIYYYASNDDPSEHQIRTYVYEINFRTAPKYEMWGMIIIFSILSMNIILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXB0</accession>
    <name>A0A8S1JXB0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0110406</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXB0"/>
    <dbReference type="OMA" id="QDLQYRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000005">
        <property type="entry name" value="Chaperone protein dnaJ 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000064">
        <property type="entry name" value="Molecular chaperone DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008035">
        <property type="entry name" value="PRK10767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF52">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="25"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="166"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="166"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8047392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="409" mass="45127" checksum="A77E1554F2E5980E" modified="2022-10-12" version="1">MQVFTKNVLRRFAFTPFFSFSSKRDLYELLGVPKNASQNDIKKAYYGLAKKYHPDANPSKDAKEKFAEINNAYETLSDDNKRRVYDQVGMTGDEQDQAGAQDPFAAYSSFFRQGARGGRGQEFEFDESIFGDFASFFNMGGEAERTIKGADIYVQMEISFMDSIQGSQQTIQFEKVGTCTTCNGTKCKPGTAPGRCTHCGGRGSINYRQGAMTIQMACTKCRGTGISIKNPCTSCRGVGIQKQATNETINIPKGIADGQNLRVTGKGNVGENGGKSGDLIVKISVKPDPYYKREGYDLITNAYISVAQAVLGDTIKIKTLNGDKQVSIKPGCQDGEKMRLNGLGINKLAPNQNQKGDQVINFKIQIPTKLNDKQREIFQELAKLEKTESQNQAQKQEGVFDQAKNAFHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2V7</accession>
    <name>A0A8S1K2V7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0130094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2V7"/>
    <dbReference type="OMA" id="CHINNEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="36"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="301"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="369" mass="44022" checksum="A856FEE77AC7EDCF" modified="2022-10-12" version="1">MFKIQQLFEENGIPFETKLDPRNYRKILDKQFTQLIQQQENEQINLEEVLQEQIQLKQQLAELIRKVHSSGKPRKKNANKIKQRKYEIKTCEAQIEQIDQQLKERQHQKQSTQEEVELLGNKITEIKEEANECREELQRATATVQELSQSYQKLVNQSNQIRNIYLNLQQEIKASQQKQNQLQAASLHYEQEFEKLCKEQQRLDPKLLQKQQKLEELEQLKNKLDSELSQKNNELQQSRMQLKEFEDELNKTKSYDKLIKQEVKAQESLQMERKLLLDEFQRICSISGIDQLAQQSDEGIMNELDQKIQQNQDTIESQEKMLDGLQSEINVLIEKEETFTIRRNELLNRYHGLEKQMDDLAKKYAQFDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4A0</accession>
    <name>A0A8S1K4A0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130337</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4A0"/>
    <dbReference type="OMA" id="QINFEYD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8049223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="281" mass="33457" checksum="72F88B4F709440CD" modified="2022-10-12" version="1">MLTPYFINESFIDQLFNQNPVSLLITRIQPGRVGFGNGIIKCENFNFQIRRLISFTYQNQKKNYYQLSFSKQFTRFQMFFQNPKSQESYYFAGFRVFPHNSFSAFIGNTQNKGYLVTKQYLDVKIFNNFCLSYFLDALKISQNFLSQATFRIFYPNFAESNIRLRTVWKDRYLFNLKYDKQPQLETGLALPLSNSLYFNVISNFFFDDQRISQITQFTQHYKGFITKIGIESNFVNRISTIPYIQFEMILSQDPCMILKGQYKNLKPSLQINFEYDSLKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4F3</accession>
    <name>A0A8S1K4F3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0130035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4F3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035808338" evidence="1">
        <location>
            <begin position="16"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="376" mass="43658" checksum="09DF5F544CED72DF" modified="2022-10-12" version="1">MRNMFILIFIGYVQSVDICVQQTTYEQCINLKGEQCKWNGQYCRWSNNYLFGCDPTLNEKLCTKQIGKLDGTIAMCIFDKTCHIIYEQYFLKCSDKLSKSACLSITNPDQLCKWEDDQCMNLNQTEFNNINAIFQNLELSASVCSRITGFLIIHHSILWKLLEYSPDILREAQRKVEIEAGVEFQESKTYQDSNLKDNFIDSNGMFIWYTLSTKQSISDTKLNDFQRYGCIAQEILIENRFIKLFSLSGTIRGVNHVYCKYLNVHPTNAIFTVFTKFECLPTTEDQLQDESYINKNQLQCQDMSGIVCQRFKSSEIKCVIDYTQEFQCRNTNIIVEIDECKTLSGTATHYNCAFVIQQYCYLDISTSKGFDIQILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7F2</accession>
    <name>A0A8S1K7F2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0140133</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7F2"/>
    <dbReference type="OMA" id="YSNNTQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="383"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="642"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="74"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="127"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="209"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8049725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="875" mass="101315" checksum="010CF1B5F222152D" modified="2022-10-12" version="1">MKKINQQMQKIAGRNSFQESPLQLEKKTLNNSTKPIKTERPQQKGKTKLIDYFKKERNSLNQAVNNIRAKLKGSKSIDNVFPLTQKSERGKNSQPNQIEQRVQTDEGNSSKRLNLKAKNLSLATVSLEKKEYQKQPSTTKQSYNQSPSSQSKPILTKSKSKDQYLFEMLKNAQKISNQYQQYFNLKQNKQSIQSDTSTNHRQSIEDMLLSQKSSKGSPQPYCSKLSKKTEGDSKSLNQGYSKTEQTPIIKQQSKTKIEESLQYDPKKKLQIVLFYKQTKYYYLYDYINQSTDNLYNFLIEQIASIELASSKVEGGGTGSTEDQHIQEELNKICQFITVSKNIPYDYYLSLPEMPLNVFQGITLQLQPLYAQSQGTKKVGLKDFILIKCIGVGGFSRVYLVKKKDNGQFYALKLIDKKFIFDNAKEVIVQNERDIMVRMENQYITKMHYAFETKFYIAFVLEYCAGGELFYHLRKLKRLNEQEAKYYFVEICIGMAYLHSQNIVYRDIKPENILLDLQGHMMLSDFGLSKPNMEEGELAYSFCGSPEYMAPEMLMKSGHSYLVDCYCLGALLYELVSGLPPFYSNNTQEIYNAILTENVQFPDYVQISDQLKDLIIRLLQKDPDQRLGQNGGIVEILTHVWFHDVDFEGIVNQTLQPPYKPEPLKYNFDEEEFNKGDAEFRKQYTFNLQYEFQNVDNANYELIDFYYERPQLEFKNRTNKVNKSKLVSEIVQKESLSPNKQMISKVKHSPQESKKVLRTNIKSEALDYFKKHNLLTKSSQILQQSLKFGHAYSKTLQQPSMSIQDLKKLKQLFDQSKQILSSDRLTTVPDQNNKNGLDRVKTEQFGNLPSPKTTTNSAANKLAKFSKLFGSEKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K918</accession>
    <name>A0A8S1K918_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180001</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0180002</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K918"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036273269" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8051249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="34288" checksum="50C3D4A7C043FCA0" modified="2022-10-12" version="1">MRHFLMVIFFLQQSILAIPNTLSEYQFSITLIVLYLYFLDFSFVVTCPQPGLIWTIRYEWIAINDQRISVINSFNMNPPSDEVFNHQLLNANAAIIGYSEQLDFQLSITQLSVDNVSVGITKITGEFENLTLIGYQILLIAFREIIDLGFFTFIPVYNRNTISLQSVNCLQCQFKELKYNYQSQLTLNLQKTFGISSITQQFQRCKMYVNKGAPIKINHITQSKISGIRMPNLKKLVRNQIFHQVIDLNFMLKFCQFLSFKQYIFQFINTLNKLNIQENCKVFIQSLLTHKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCT3</accession>
    <name>A0A8S1KCT3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA pseudouridine(55) synthase</fullName>
            <ecNumber evidence="2">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA pseudouridine 55 synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0200132</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase Pus10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCT3"/>
    <dbReference type="OMA" id="LVISCQR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2510:FF:000001">
        <property type="entry name" value="tRNA pseudouridine synthase Pus10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3190:FF:000001">
        <property type="entry name" value="tRNA pseudouridine synthase Pus10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039894">
        <property type="entry name" value="Pus10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048741">
        <property type="entry name" value="Pus10-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048742">
        <property type="entry name" value="Pus10_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01213">
        <property type="entry name" value="pseudo_Pus10arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21238">
        <property type="entry name" value="Pus10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21237">
        <property type="entry name" value="Pus10_N_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pus10 N-terminal eukaryotes" evidence="8">
        <location>
            <begin position="106"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Pus10-like C-terminal" evidence="9">
        <location>
            <begin position="317"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00075270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00079393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00083669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8053049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="571" mass="66719" checksum="A608D8892C73756A" modified="2022-10-12" version="1">MQLCESKAILFNKLFEKDACIRCILRVFKITEISTYRETQQFIQLLFDVGKLIGNENKLVEEYDFLYYKRKREIIDSDVQPQTCHSSLKDQFIKNPFHTFDKINKCLICHGILQCVDDQKFISILVDEIQQKQYQFEAFKFQVKTPIGCQIRQLIIVNQCISEIGQIDPLYEYIRTPNFATDIKICVKWIMCSLLSEFLKVPSDPNDDRFQICLNFENIGDDVKQFEQFKQLISNIDDQIWEEKITKKLKMNDPQLKAETHNRLLQKKNQQNQPFLPTQNNLEKISKLNNNQISYYQIVNVNYPVKLEVTTSYQNIFIKGNYIKLARYISQTPWIIDGNKIYEDSIEDLVSAEACKIFKPSSVKFHSGGREDIDVRMLGNGRPFAIELVDPHQGLQNQTQEILNQIEAKINSQNVVKVTPLAYTDTNIFGDLKNSETNKVKAYCCIVECKNIINEDLIEQANNIKDLVIKQKTPIRVLHRRTLMVRDKIIHSLLIKKINEKWLLVYVLSSAGTYIKEFIHSDLDRTVPNLCTLLQNECDIYQLDVIKLYEKINEDVIIDFKNLDKECGCKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KG04</accession>
    <name>A0A8S1KG04_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0200191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B box-type" evidence="1">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="144" mass="17306" checksum="AA71CDA6B211BA6A" modified="2022-10-12" version="1">MSQSHLLEQLPQKDNQGDQCSIHSKPLELVFQEDGKYICDNCALFGNHKFHNYKPIDEYVKKMEQTFYDITTMYEIVKEMRAKIEQKTYFKEFETQLLQSKDAKLQSIDIKFNELFNELMRMKEEIKTRISNNYNLLYDDXTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJK9</accession>
    <name>A0A8S1KJK9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJK9"/>
    <dbReference type="OMA" id="KTENDVH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="68"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="129"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="618" mass="73699" checksum="5E20407F9F24478B" modified="2022-10-12" version="1">MNRIVRQNQRAAYSLNQSMTNGLPALTPDNMNLCISQFYGRRPTQKIYDSPINEIQSGRQQIPYQKDTEQLKEEIIQLKKENSRLKDQNKQLQFQIKLDEKEQQDKRTTQEVSDDQINYNQNSRLVEKLKQARMLLQERQTEIESLKKSTRYMKLQEMEMELGNTKSKFEILNKQINEILKTENDVHKFHKLTIQTVELEEKLRILSNSNQKQKRKILCLRQELLTCQAMKDRYKKQLEQCQIEFDKYKKNHEQEIQKKTRYKDKEIIDNLNQQLQNKQGVIQSLQIENEKQKLLIFQKQRQFKELEDQYQTKKSWLTNKKEADTSFQEVLDEKVNKNLLVLGDDDLSNSYKQQSETKIILEEDHRDRSYTMQNRLSVDSVRDEQKSPKVQSLQIIPEIEVTKQKLPRVNHSDVEEIGKQLKYQLMTQHVAFEQINQFFEWKSEISIIELVDILQGAPFCMQQQKQALLLARYLIEDNTQPYVEFTPLQTNDNCVIKSVLKNIIGKYQLFDNEQQNQIMQNISQQIWKFRQSIVEQIKMITSKRSNSQYNELCDEIEFKNAIQGSNILLDERQKEFLGFFIFREFDGSKQFDYKMLIDKIGNQNPQNSPSKINPQQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KMN3</accession>
    <name>A0A8S1KMN3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KMN3"/>
    <dbReference type="OMA" id="LNTNYQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="110" mass="12474" checksum="51C46DABBAF50C88" modified="2022-10-12" version="1">MIAGYLQNKLIAQSSIQEVHGTDMLATQDRRTSKIYMGGQSHQQMNPLDLVQISSALPQFSLQQNVDPILQNLLNTNYQQQISQLASPPNIELINQIKEQARVVRMHYQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KPD5</accession>
    <name>A0A8S1KPD5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KPD5"/>
    <dbReference type="OMA" id="IMPNYMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="219" mass="25638" checksum="16EA1267E7F4AB3F" modified="2022-10-12" version="1">MQNPNFMPILLYTQPTGYPQYYPMYYMPIMPNYMIPQYQSSQEPEINQIISKSSDQSQFSNQNCNLNNQLNFQSIKQNNNEIAVENNEEIQTNTESNKPKQKSGKKKINLLQKSTNIQKNYAKAIISYACGQRVLIINTLGKKKGIEFLKIINQLKNKLKNINHIKNYTQKESFLSMFRILGNKFLKNEAVSYIYHSNIQQKSCHLKHLKMIEQNLLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQ05</accession>
    <name>A0A8S1KQ05_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0240004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQ05"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="168" mass="18993" checksum="165B9CC4E8A104CB" modified="2022-10-12" version="1">MLIMQQNYQLGLVNKVDTCIPQYDFSTLTGTQQICLRYPGYSSNSSTATDTTPNVQRKFSDNTEESDNATCASFLPGCISFGKDCFDFTIPSTLMKETQETYDKIFACTTGSIDNFTTNQCYNSSTATENDYCIIKICKLPKKENDGTCGFFRGHYQRLYRQYGCNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQ94</accession>
    <name>A0A8S1KQ94_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0240383</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQ94"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8056581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="90" mass="10957" checksum="32F7B400BED869CD" modified="2022-10-12" version="1">MFYDIKIFKLGLQEQVIESWDEAIIKMRKYKNFMLKKLKLQMIKIDIKRLFKIEILQSILNILEKQSNIREALKISRINGTIRKIYQKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KU58</accession>
    <name>A0A8S1KU58_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0250092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="170" mass="20201" checksum="00BF10718DDA190A" modified="2022-10-12" version="1">MFXLIRKYLISIIQRNKNNNYQEKSLQQQLNNRFCNVVLSTDNSIMLPTIQKTNSRMSQKSNYKQRLVLGSHSVQRSDKFLDRLQRSDTKNNDNSFIDYDENRRLRIEQRKSSQSIDQLNIGCQTSFIMTDDKKQESVQYSENFDSYCSNYQEESSDNQQKNKSKQKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVT8</accession>
    <name>A0A8S1KVT8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270263</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVT8"/>
    <dbReference type="OMA" id="QIGDHEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20566" checksum="0C6BA8912C0DD5D8" modified="2022-10-12" version="1">MNQFQSIREGYEQIGHEQYYQEYGSNYKNPHEDKIKQVLLIALAKWKPKLIKVLDLACGSGEITLILKSLGVKNIVAMDPYTMQAFKKRTGDECLPISFEQIADGQIGDHEYSLIICSYAMHLADDSLLPGLCMQLAMISKHMIILSPHKRPVIKWGWNQVDEIIKDKTHARYYTSTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXG8</accession>
    <name>A0A8S1KXG8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXG8"/>
    <dbReference type="OMA" id="CITYQVQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="210" mass="25221" checksum="5F6F730360D7A2EA" modified="2022-10-12" version="1">MNFHSNWRYEKMQLIKDVLVNKNVVPIYIQNRLMKELDFQIKAFANKNKNSLSPKKGCNRRIKTLDQSPQRIKFERSVSRSYNKDQDCLRCRKFNFQNKIIIQCDTGQHMFHEKCLLDLFKEQLQDIQPHFSCSCGMKLCPEFVKELNIRGIEQLVNQLYEQQLKCITYQVQIQNCLNPQCNFFWITNSQKSRKYGRQLKPLTKKYCPYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KY07</accession>
    <name>A0A8S1KY07_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KY07"/>
    <dbReference type="OMA" id="CQVIESG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="568" mass="67149" checksum="C960904B776257B0" modified="2022-10-12" version="1">MRSSESNEVCKRVRLNDQNWQVGRFLEEGVEQRFRNLRQTNFLVEYIKDGLIKYIMDGEILRIEKLYEGYNQTEVIKNVEQIKYLRWEGEYGGNQKKVGKWNAFWRGDNLNVGGHYNENGQKQGKWIELFTNFFEQIKKKIELVQVKSLVLVFINKIRDMESGQQVLKIKLLLVEYMMRMAKKMVFGLIFMINLASKYINGLKFGRWDTYFQMSFEDEIQLIGGGEYNQNGVKSGFWIDIHEDYDYYEYQIIYLGEYIDGKKYGQWNTQFKDPNFLDQQSFIGGGFYDQNEVKTGKWIELYEDFRNVCQILLEGEYVKGIKQGKWNTQFRLNQHHKFKTIGGGNYDHQGIKNGRWIEMNNNFWKQCQVIESGEYQNGNKIGSWIIKFRYTIKYPFNKIGGGIYDNNGMKNGKWIEIFKNFQNSSQVTLVGDYLNGTKTGRWNIMMRYHEKEKFQFIGGGMFDQQGLKNGDWIELIENFGIQNQVIMKGEYLFGMKLGQWNTLYRNNPKLDFSIIGGGQYDKNGQKIGYWVDLNNYFGNEQFGSLQVLKGTYENGLKKKKFTHNFLHQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYR5</accession>
    <name>A0A8S1KYR5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYR5"/>
    <dbReference type="OMA" id="TWLNAIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="391" mass="46427" checksum="ECE84C097ED49028" modified="2022-10-12" version="1">MKNLIKCQDNQILEDDEEELENTNNQKKKLQFINRLQRKNNKSFDQTWLNAITQENKNEIQDKIKLAVGDSKFKFKYLFQIPQIINNPKLVQRKKTYEIFTKNSQLEQYYLQLKKASSGAARSISSKADYKDILSQISNNKVHDTYQHQFKQNNGVIYTNELWSQKLRNQIYDQSRLKELSELLEMNKIKMSYEEQLMNFQEYFVKKLKPKKQSITEMKQIIESAFFSENLQSQKSDTNKEVKIQHKSLISYFKSQNEDQTQDFSTKHSRMASRNQSQGLRPQSQEMSFLSRRASTSQQNTRMMMVPQFSALNSPVNSPVNRLDQIVQDTKSENQSTKQTIKQINNFIKKENEIQEKIRDDRMKKYRVQALNNAIKLKKNRETNSHIRNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZ84</accession>
    <name>A0A8S1KZ84_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0300039</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZ84"/>
    <dbReference type="OMA" id="WRIQIFA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER 16, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184:SF12">
        <property type="entry name" value="SUGAR PHOSPHATE EXCHANGER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8060247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="54871" checksum="1E85BE4E43067084" modified="2022-10-12" version="1">MFDSAVPGLKAKRIQVFICCFMQYAMLHSCRSTWSFTSGLLAKQDDHDHDDEKIDDFDKKYLGYVNFSFLFVYGSSMLFLGQIGDRIGPKLFMLCGTFSTGIIQFMIAALLPVEKHNRWLLLVLQILNGISQAPAWPGLMAIMNNWQSKPNKMIVMGYFAACTNVGNILGDLFAALLIEQLGMSVMAPIYVSSIGVLIMSIINIYMIEASSAKEYIAQFKNPLTKSLVYKQAHDEYMSRAASVISVSEQSYLTDLSQTLLTVDRDSQMNPHVPPKPKKNKKDSINMISAWKLPNVALYAFAFGCIKAVFYILAFWLPSYLKGENLEGVAWITQMIEWGTIPGGILICYAGVHWNLRASLIVPSLWIGTVVMVSINFLSKLEQPYALYYILVFLTGLLIGGVYNNVSGAIVIELSNMKELKGNKKSTATVTSVVMGYGAVFAAINQLIVPYLESILFLYCSLNAIVAGCFLIPLIINEYKRYKEQKLKVGDAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3M1</accession>
    <name>A0A8S1L3M1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0320317</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8062077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L3M1"/>
    <dbReference type="OMA" id="GLKTCHS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8062077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="352" mass="41954" checksum="E3B65A3FC4036360" modified="2022-10-12" version="1">MNYVIKQTEQFLRGLKTCHSIDQNLHCYFSKNGILLLSNYQQFEDVETKKPTFFIYFNSNSFEQLQTNSIHYKMPQTLTPDLQIKLQKLNSTLDKLAFELNILQLYTSLKPFKKQIKKIEIQVYYTISQLRVIQKFKMIVFFKDSFFTIECPIIQLDEIEQMELIRKYMEKRRHLEVLEVDPKISEYLNDFNEKEALQFILKRNTIEIHLNSQKQETSLELNSGFLTQYKFDDFISIYKNVNTINIEPNILTPFHGYGFKISISKDVNQKARIFISQDWQDQISMVLKSITTLTNKIKVISILDDDCNDDELLDIIAEYQIDRVQEQESVMQIQQVEEQQQIRKKIKMNQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4Y5</accession>
    <name>A0A8S1L4Y5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0330186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4Y5"/>
    <dbReference type="OMA" id="NMIIIDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="94"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="345" mass="41544" checksum="CACE388A2FF7B7BD" modified="2022-10-12" version="1">MFCCLGRKKRDNNEMEKKNSKKEMVERFILQNCKSNDEDSFDESIDKSNMIIIDYDNNQYEKKPWSGIQWQMIIQTKNLNQIELDELKRNIRFGILCNLRPATWFWITDIKNTQMKKHTSNIYQKLKSCESQYDSQIFKCIKQLDKKYNDLVFDILRAYANYDVEIGFIKGIDQIIEYLVQQLDPEQYINLDYSFNQFNRMCDFYEQMVFWMTIHILFHLNYRRILKQNVSFIETEKFSNTLMPSINSLIQQLNVKISELIEVPLLFFFQKQLKKEDFNRLFDVFLFEGESILLQILLKFLKISEDLVCQFQAEADLKIFLNQTLIPYGISKIEKINQKLMNFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6R0</accession>
    <name>A0A8S1L6R0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0320231</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6R0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="161" mass="19056" checksum="F0C07D2C9732A875" modified="2022-10-12" version="1">MNFKNAYDYLLNFQSQTSRNTSRITKSEDSQFLSSQKQCLQNSEQKYRVDNSKQNNRNLFESKSDVKINLGENPFDLATSINYQNLQQSKQNNVNQQGLQLLQESNRMDDIYEIKIRTNGPIKEDILSFLQLLGQVEIRKIENNQDKDFYYRQSKSFHTQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6X4</accession>
    <name>A0A8S1L6X4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">40S ribosomal protein S14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0340179</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6X4"/>
    <dbReference type="OMA" id="KWGVAHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000002">
        <property type="entry name" value="40S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8063159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="17939" checksum="F0939A06DF1C488E" modified="2022-10-12" version="1">MADAKPQPVQQPKGKKGKKTGEETVQQVVATLGPNVAGNELVFGVAHILSTWNDTFIHITDLTGRETLARVTGGMKVKSDREESSPYAAMQAAKDVYEKLKTLKINALHIKLRARGGVDTRQPGPGAQAALRALARLGLKIGRIEDVTPIPTDSTRRPGGRRGRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L8U3</accession>
    <name>A0A8S1L8U3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pyruvate, phosphate dikinase</fullName>
            <ecNumber evidence="2">2.7.9.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0350225</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050242">
        <property type="term" value="F:pyruvate, phosphate dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010121">
        <property type="entry name" value="Pyruvate_phosphate_dikinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01828">
        <property type="entry name" value="pyru_phos_dikin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE DIKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931:SF9">
        <property type="entry name" value="PYRUVATE, PHOSPHATE DIKINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="6">
        <location>
            <begin position="21"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme mobile" evidence="5">
        <location>
            <begin position="452"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="6">
        <location>
            <begin position="692"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme mobile" evidence="5">
        <location>
            <begin position="933"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme C-terminal" evidence="7">
        <location>
            <begin position="1029"/>
            <end position="1376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8063957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1383" mass="153210" checksum="E087879DC31D3D1D" modified="2022-10-12" version="1">MSKYIYTFGDGKAEGSAAMKNLLGGKGSGLAEMNLIGIPVPPGFTITTEACNLYTKNGAENTFALIKADVEKALHQVEELTKMEFGSAENPLLVSVRSGARVSMPGMMDTILNLGLNDVSVEGLSKRTNNPRFAWDSYRRFIQMFSDIVLDMKPTTKEDIDPFEEIIEGVKHAKGVKQDTELDVDDLKTLVTKYKEAVKTKTGKDFPQEPQQQLWRAIQAVFDSWNNDRAKYYRILNKIPEDWGTAVNVQAMVFGNMGNTSATGVGFTRDASTGENIFNGEYLINAQGEDVVAGIRTPQQITLVGSKRWAELQGVPEEVRKAQFPSLEESLPETYQELKNVQEKLEQHFKDMQDIEFTIQNGKLWLLQCRNGKRTGAAMVQIACDLFREGKIDEKTALKRIEPNKLDELLHPIFTPEALKTAKFVAKGLPASPGAASGQIVFFADEAKKFAQSILVRIETSPEDLEGMNIARGILTARGGMTSHAAVVARGMGKCCVCGAGSLNINYKQRTLEVNGKVYAEGDWISLNGSTGDVFEGKLQTQEAQLSGDFGLIMDWAEKYTRMYIHQKMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKTGKDFPQEPQQQLWRAIQAVFDSWNNDRAKYYRILNKIPEDWGTAVNVQAMVFGNMGNTSATGVGFTRDASTGENIFNGEYLINAQGEDVVAGIRTPQQITLVGSKRWAELQGVPEEVRKAQFPSLEESLPETYQELKNVQEKLEQHFKDMQDIEFTIQNGKLWLLQCRNGKRTGAAMVQIACDLFREGKIDEKTALKRIEPNKLDELLHPIFTPEALKTAKFVAKGLPASPGAASGQIVFFADEAKKFAQSILVRIETSPEDLEGMNIARGILTARGGMTSHAAVVARGMGKCCVCGAGSLNINYKQRTLEVNGKVYAEGDWISLNGSTGDVFEGKLQTQEAQLSGDFGLIMDWAEKYTRMYVRTNADTPKDAQIARKFGAKGIGLTRTEHMFFEGERIKAMREMILASDLAGRQTALAKLLPFQRTDFEGIFEAMNGYGVTIRLLDPPLHEFVPHETAQQQELAQEMGLTLDQVKQKVHELAEFNPMLGHRGCRLGITYPEITEMQTRAIIEAALNIKAKGLEVKPEIMVPLVGNVNELEHQLKVVRKTADAVFKERNDTIHYLVGTMIEVPRACLVADQIGQIAEFFSFGTNDLTQMTLGFSRDDAGKFLPYYITNGILKVDPFQVLDRQGVGKMVELAVKNGKSTNPNLKVGICGEHGGEPSSVEFFHIVGLDYVSCSPFRVPIARVAAAIANLTNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCA3</accession>
    <name>A0A8S1LCA3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0350047</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCA3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="274" mass="32536" checksum="67B9691A028B27C2" modified="2022-10-12" version="1">MDQVVKKKQEQLLQCLKKISSKPQTDDEDQKQQLRKFQQEVEQKYKLARHNNEYRFPFEYYDESSDSSLEELQKVGTYDLRKQYQKKQTNSKHLQSSKESIKAKSLIKIMSDKSMKANRQSTRYKTEQYDENQVSYIDALKKNKSIEKQTNEQIISFIRKSVAKRSQYSQHNNNNNNTSRQYINLSSNNQSHIMSQSFNGQKSRTQNYYLNKFTKLKQDMFKQQNQTSTSQMNQDSKISLKYFFGSKSPEQSCTINLKNKLAEMCKRQPLKVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFG5</accession>
    <name>A0A8S1LFG5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFG5"/>
    <dbReference type="OMA" id="HEKTKIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="262" mass="31167" checksum="5531D9462A4951F1" modified="2022-10-12" version="1">MNNQFLPDDDDPQVKEERKSLYKIFGKETSLGKELFGLYNAHEKPKINYPKPKQKTQEQLDAEKMKTQKSCPQKTVIDYPKEEPKQKQQYYPIDFIQKRKPEAEIRKEIEKYYEGRKFFRPAVQGQDRKKLIEQLQRVFKYKRGALPKGAELPEINIKNDDAFLKDSETTSNAIKKMHKKDLYFTGLKSDNQSDATTLQGDPQIELEVLFSSIMKEIEERQEFLQEIEYLNEPKLKQRIKDEIIERVAELQKVQDLRRHYCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LI90</accession>
    <name>A0A8S1LI90_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0330229</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LI90"/>
    <dbReference type="OMA" id="QHLAMEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000082">
        <property type="entry name" value="Shaggy-related protein kinase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF0">
        <property type="entry name" value="PROTEIN KINASE SHAGGY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="78"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8062624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="414" mass="47693" checksum="F0CB35C9A272601E" modified="2022-10-12" version="1">MARYAKKPINKYTQPTDANRRVQTEAADIDDIDLQQAILKFKNLQEKQKTPLKNQRTKSKSPAPPQNNQPIDYSQFTFTSLKVIGQGSFGIVYKAKVNETGEIVAVKKVIQDKRYKNREIQILQELDHPNIVETKHAYFTYGDSPDEQYLNVIMDYQPETLHSFNASFLKQQQLLPEIQAKLYSYQLLRGIAFVHTNGICHRDIKPHNVLINPDTNVLKICDFGSAKKLSPLEPNIAYICSRCYRAPELLFGATNYTTQVDMWSVGCIIGEMFNGLPLFLGASAVDQLVEIIKILGSPSKDEVLSMNELYDIKQYKIVQIRKKDWRKVFQTVVDPSAIDLISKILTYCPKTRLTALQALTHSYFDDLRDETTFRMYQSKIQIPDLFDFTKEELSNNQSLANKLIPKWYQKRNKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJ41</accession>
    <name>A0A8S1LJ41_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0390298</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJ41"/>
    <dbReference type="OMA" id="YAWQIEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000121">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="205"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8066789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="410" mass="47911" checksum="51D9B1A14261DA3F" modified="2022-10-12" version="1">MNLSRNGSNKFSRHDRIAYSQILDLKKNKIYSSSTKNSDQSNGYFLSLANMKAPDYGQAQLLNNDALKNKINEKRNQKSMDNELNKFVTRVETKLISSPLKKKVIAIDTKIDMPTQIKPYSLTPQSRLQSFRIASPSQKPRSSSQNKRNEIYYLSKLQRAFDTNGSDYFSRMYRQHFHQTYQGLNCRLFPQNNNDYNKSHKLPKKHQRQYTLFFDLDETLVHCNETPSIPCDVVLEINVSKHQIVRAGINVRPYAKEMLKNLSNYFEIIIFTASHSSYAEKVCNYLDPDSNIISHRLFRDSCTQTNTSLYTKDLRLFCENTNRQLSQVALIDNASYSYAWQIENGIPILPFYDNKDDRELIDLEKYLKNMIGVSDIREYNRNNLKLHLFVDQRGPYKVLENLFGKPQQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LMN5</accession>
    <name>A0A8S1LMN5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein/NADH dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0380109</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LMN5"/>
    <dbReference type="OMA" id="LVRYKYG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016464">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B8 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005822">
        <property type="entry name" value="NDUA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8065816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="100" mass="11716" checksum="7893106CDD715013" modified="2022-10-12" version="1">MSWYKQLGNSVKELRFVFCQTCGRSEGIRNLVSKNYWQWKDANPHFPFVVRECESIDPYVLIRYKYGVEKKALIGNLNEQELEQVLGKLVDQSNKVNSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSU6</accession>
    <name>A0A8S1LSU6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0470261</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSU6"/>
    <dbReference type="OMA" id="MMEYNKE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22749">
        <property type="entry name" value="Otubain_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1300.20:FF:000006">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019400">
        <property type="entry name" value="Peptidase_C65_otubain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931:SF15">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTUBAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931">
        <property type="entry name" value="UBIQUITIN THIOLESTERASE PROTEIN OTUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10275">
        <property type="entry name" value="Peptidase_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="203"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8071598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="406" mass="49009" checksum="0C09B0189BDC5381" modified="2022-10-12" version="1">MEKEIISRLNQFFQKIEIFDMRQKVIYKRLSEVEEFQNSFIQFLQIDGSFSYTKLSDIISQTQQQNTLDKQNNSLIIELEQNFNNQLKFIQDQIQQINKNEELEQSWKDKIDKDIKNISKLIEEIQQQKQQIKNIDNDRNLQNLNNPQPIKNVIFDIYSKTTKSRVVQGEFQYFIHQIQSQSELGKYYNLKLDQKKKIYSQISGFRQVRGDGNCFYTSFGFQYLQLLIDQYDQNEYDQFLNSVIKKMRFRIQYPNNKIEDEITETNLREDFIKRLNFIRDIKNKEMRIKELDKQFRAYEQEDELVDGCFYALSTIFFRNLSLYFIDQSEYKDNFLDKDTLLQWEVECNNNEIVIALLAQQLKINICVFLFDKQQFQLMEYNKEAKQKIILLLQPGHYNIGIPKIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTG0</accession>
    <name>A0A8S1LTG0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0460148</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTG0"/>
    <dbReference type="OMA" id="YNQEHKR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46915:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46915">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="156"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="6"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8070807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="457" mass="55465" checksum="C9726C4D76F72F1F" modified="2022-10-12" version="1">MIKPKKKQQQNKMEQLIQNKRQEHLELLNQLEKLEIQQSTKEMQIIQTITQQQKKEHDIEIIAAPQLVSPKWLMNLKPNMTYECDVVTQEESELDQIVKHQLKEKIQKQKEMQQQAYLELINELDLQSKHHAYDEKVKEDFKCTYQYMSQGRLVSIDIKYYDFLKLNPQNYVNDTIINFFLRFIENDIFKNKSLFIYNTYFCTRLLSFHAEYKQIYTQYLQNNQMLQRWTKDNIFMKQYILFPLHLREHWAVIFVVNPLQVCEQLCNNNYQLSTDVNKNGYLIYFDSLLIQDQRIGIQIKFYLMHVYNLEHKRYSDDQIYEIVMRSTVPVHQPIVPRQTNLVDCGLYMLEYVERFLMNPYQILNNLEQDHLKWFPKVMIFIKRILIKKILNALSSGQKDYAIRYQENCRMIDQQFTDSNQYDYIDEQLLEQLQVPRLQFHLSEDYNYYYDVSPSVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LU94</accession>
    <name>A0A8S1LU94_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0460139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LU94"/>
    <dbReference type="OMA" id="QDQAMQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="253" mass="29522" checksum="46F58ECBB5AB6B93" modified="2022-10-12" version="1">MNQTRDLEILNHQLNNQNNKMSLQIEVLSQQLYLLQKQIHQLQEKAADYEKLKAENIILKKDIMYLQEQIQSQESEQLLVQESKFNLQIEEMQKRHKKQIQDLKTLYEDKIISLQKEKSSMQDQAMQMGVSNQASKTLEQQAAKIIQISHKLEKTLSDLPSQSPSHGHIGISKEMYDQMLEQLKSKSSKNIILEKIPDQQNFSQRYKKSSITNIKKENNFSVKRILHYSKPQDSPRTQNSINALTISTLTKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUF7</accession>
    <name>A0A8S1LUF7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0460295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8071097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUF7"/>
    <dbReference type="OMA" id="DGHKEWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045182">
        <property type="term" value="F:translation regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002697">
        <property type="entry name" value="G-protein beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045223">
        <property type="entry name" value="RACK1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19868">
        <property type="entry name" value="RECEPTOR FOR ACTIVATED PROTEIN KINASE C RACK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="13"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="65"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="195"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="306"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="334" mass="37701" checksum="E1C5375B1FC7030C" modified="2022-10-12" version="1">MANQVEFKQLGFLRGHGDWVTSIITNQDPTLADLVISGSRDKSILVWKLFKQPDGDLAGQPRKQLKGHSHFVSDLVLSNDNKYLLSASWDKELRFWDLLNGTCTNRFVGNKKEIFTCAMSPDNRQILCGGAERRYKLYNVKAEEKLTQMNHFHSDWISSVRYSPIIKNIQPYFVTVGWDGWLKVWNQNFTIRFQFKAHDSQINSVAINPSGEYIATGGKDKKLYIWNITDLKKPAFEYDAGGIINQLAFHPQQNWIVAATENGIKVWQISQEEKAETKTPIVTLDHHTETAVAGGVKKIQKHGAVSVALDANGAKLYGGFTDGSILVWEVSTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZL8</accession>
    <name>A0A8S1LZL8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0490255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZL8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="98" mass="12170" checksum="8DB33FFB20E0DBBA" modified="2022-10-12" version="1">MLSGQCIELMFSQQLFNCKMWRQYYDRGFIRKVMMEMINHMMKIQCCVECEQNQCIMHEDKFYILDIELKKFCKQIRIKMIKTLSSNYKFKEYFTLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0B0</accession>
    <name>A0A8S1M0B0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0480159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0B0"/>
    <dbReference type="OMA" id="WCPKRSE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="179" mass="20953" checksum="86A5E957D6605BDF" modified="2022-10-12" version="1">MGQENSNAKLSQNFIVFGVNLLLDEKLMGSEWQKDQINSCQDFTLYKNISEKPNQLLWCPKYSEILQNTVESIILNIEFEGVLYVFQHTQDQKILNDHRKLLKWLMSEDSMRQCSLILLCVSQSNDLAINIKSQMEIELDLESMDSKQARNLIIIQKDKESHLKFQKIIQQLIKMKIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0Q4</accession>
    <name>A0A8S1M0Q4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0480234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0Q4"/>
    <dbReference type="OMA" id="HKESCIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="184"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="247"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="573" mass="67235" checksum="E02FFC74FF62A290" modified="2022-10-12" version="1">MQQKVHLKSLDDACEDPHLHEAIKLFLTMNKDNHTREELYFILQRMEELPYFKQFVAEKLQKGTQRNDILELCSRLRMEYFKGGEVLFEENDPSNDKLYIIFYGDVMLMRMKKMDQLMTLQRQNSEAAPQIQNQQQQFKISTILHAKRLTHKIVKHHNHHKESCINQEEKKRLEQSYGECMRILGEGNGFGEKALAENIPRTLTVACYSLESFIVVLKKDDFLTYQMTFEKTKKEKQQLMFNIFKNVNNEYSSQRLESMIYSCQTIQYDRATQLTSEDESGDSFYIIISGDVTVYKRVDNKNIPLCIVSQGHLLGEEIVINKSANYEFTSQVTSLQALVIVIDSQEFIHKFPEECRIQLEHDYKQKSRNRQNLVKLLCQKKKIDTITNGVQKDQILKLAEIDNYYVSNRTSFNSAKKSDLKLNSNFLMVYSNPSVQNYCIQSIVEKEKVFSSIQLNLNPINNNQNEPSEFNLGNLSKDYLKRRKILFQSKLNQKLPLQNFLKPSSRVLPMSIKRDVFRNYKSNKNRINFHSSVNIFNYASPVSQNKSNSKFIQYDCRESSTNIYTQFDDKQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M359</accession>
    <name>A0A8S1M359_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nuclear cap-binding protein subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">20 kDa nuclear cap-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0520078</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRM NCBP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M359"/>
    <dbReference type="OMA" id="QIYEHMC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12240">
        <property type="entry name" value="RRM_NCBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001933">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027157">
        <property type="entry name" value="NCBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034148">
        <property type="entry name" value="NCBP2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18847">
        <property type="entry name" value="20 KD NUCLEAR CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18847:SF0">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="40"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="127"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8074099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="194" mass="22345" checksum="D371248841E97B12" modified="2022-10-12" version="1">MSNLNAASLYENLQQPISQYYDKSSNLTREEYFYKLSISTTLYLGNLSIYTTEEQIFELFNRVGAGVRRLIMGRNKETGKAIGFCFIEYFNHEDAKEAHEYLDLLKLDERAIRVDWDIGYSEGREYGRGTGGNQVRDSYRKVADPERPNVQQNRGRGRSYNNNGGRRKQADEDESDNSGGGYKKRAGNRSNNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5B1</accession>
    <name>A0A8S1M5B1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0480056</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5B1"/>
    <dbReference type="OMA" id="EIQIFHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13431">
        <property type="entry name" value="TPR_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="34"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="170"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="538"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="572"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8071796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="825" mass="96843" checksum="4E19247342F7E580" modified="2022-10-12" version="1">MGCGSSQTEAPELQKGHHSQKTNISSPDSELADKQKLYQRGNYLLKKGKYDQAINLFDRVLEIDPEFADAYYSKAIVAFEQNKIEDAQNLLQITLEKQPDHVYALNEAGCLMIKQRKFTEALDYLEKAFAKQQNFSEVNYSLAYALSKLNRKEESLKYYDLAIQEDAQQKHFYINKATTLIELNKYEEALKCVSEALKLDSNYIEALITQGNIYKTTQQYEKMYSVCLAILKLDNENKLVLQMKQEAEKNLNIQITDQQSSTIISIEDKLILAENLLSEKQFIQARKVVDQILLKDPLHYKSLRLKAKIQVEQGQYGDALASLNKMLNEQPKDIDVLKEKAFVLEKLKRLEDALQCHNQIIEEETNQTKNEKKANIYMKLGKVKEASEIFESLNIKPNLDDPQTYIFKAKLLLSQEKFEEGMSLLQEGLEKNKNNIEIMQLLAQFYKAQKNEKEALNIHKQILEIDKNNDCSNFEIGVIYCDQNLYKKALESFKKIKQLDFNELCYFYFGLCYYKIEDYKNAIKNLSNYVKLGRTKLEESYFMLGGANLFQLKYDEAEENYLNCIKQNPNNSEAHYQLGNVFKQDKQIEEAKKYYELAHNIEPYNEIYKQTYETFINEKNYLVDYSNSLLFTISTFIGICQLNQKQQDYDQQKIEKYLSILLLLETHQKKRFNSIENHFEQIKQLIAKSYDMQYTVIDDTLFPIMQYWHNQKICKKKKDINDFVNSIINAVKLLVNINSEKILNVINSERTLNNKYCIEFAEKIQHESYQKRSGCLGLIDGIKILGFLLKYHKDFTKDRMVFQELIATKYQIEEIQIFHESYFKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8J3</accession>
    <name>A0A8S1M8J3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0520114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8J3"/>
    <dbReference type="OMA" id="YINEENW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="311" mass="36333" checksum="96CD390B5781A3F6" modified="2022-10-12" version="1">MLNRAINKGFRKALPQFTFSSRNGPYNPNRYRHYLNPNFFQSNKEIADIAKSQQVPLPVRNVRHVNPVRQSGPLPPYDGPYTMEDVRAVWQNTSIGRQGTWSCQMDPDEIMRRVPGITRREVEKILNMGLTPQEQVDFAYLVYNCGFDVDYVPNSVYVARQVVTNSKGEKVEILWNVQVLEDLAKLPVGFAPVRELIDYHWEIFLWSDPMIKPTGDLDLGVPNTWFEYECEWGGEMVGIEDQFNIPESDRVFPSPKNPNCRRELWKSQDDIQEQLEMEDENWYPKGTSYNIYQQTDYKKVTKSDSTFQEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9N2</accession>
    <name>A0A8S1M9N2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0520250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9N2"/>
    <dbReference type="OMA" id="KERHYEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="325" mass="37891" checksum="FCD41819170BF775" modified="2022-10-12" version="1">MTNQLGVGFGAYKFQTIINNEMRVTKDSVHRTRIQTESRKSIQDKCKYQFQNAKKKIIQKLQDPNERERFNTLIQVDKDSDDSLSGDPENQAAYLLQQKKKKEKEFELYVSESSSFSGIDSDDSLNEPNQEDIKKSQIYEEENKLASQNLKALNTPTILKSPQISQSRAQVALQKVKAMKNTRYPVVKKDIDFLRSLCQNQNFVNNEKAILEEYKLDKEICVDMDCQDIVTFMRLQHEVEEMRRKQTEFNTQKERHYEVTKNLPKKSINKSNTKAEDNGFCTVMMAKRFIKTLKLKVEQKQPKPPIQAINLLRKSTRGSTLKTQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9X4</accession>
    <name>A0A8S1M9X4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0570178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8077030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9X4"/>
    <dbReference type="OMA" id="VYEAEFR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="161" mass="19330" checksum="F9D569A89A8D467B" modified="2022-10-12" version="1">MQVIQEMIEEFNTLRQQVYEAEFRIQSFIDDEQMSIKQQKNQVDENQSKIQKQNVKIQNDQLNKITLALTNSLTRVIKDLYYDEDKDLIIIKLQEHGIVIELEMKQNQLKRIKLQQVGRRDYTLIDSEHLNIEEMHLKFLERPDQPFSSIIIPYLENLKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MA09</accession>
    <name>A0A8S1MA09_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S acidic ribosomal protein P0</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0580068</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Ribosomal protein P0 is the functional equivalent of E.coli protein L10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MA09"/>
    <dbReference type="OMA" id="DDAMFGL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030670">
        <property type="entry name" value="uL10_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039087">
        <property type="entry name" value="L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="6">
        <location>
            <begin position="129"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR039087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8077348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="323" mass="35220" checksum="920BF9D5C3714F5C" modified="2022-10-12" version="1">MGKKETKDKKPAQFKKIYELLSKYTQVIIVGLANVGSKQVQDIRRILAKRNALLVIGKNTLFKKVLATRVQELPKEHEYYDDLAKFGSAIKELDALKNSVAGKVGFIFTDTPVFDLKPIIEENKVETPARVGAVAPIDVVIPPGPTGMDPASIQFFHALQIPTKIEKGQIQITKDFVVLKTGQKVGQSQAVLLQKLGKKPFLYGMEVLACYDNGSILNKQQVSVNLNDIVAKFQQNVRNVSAISLANGWVNESSAPYLLANAFKDLAAIGLQSGFIFDQIKQSSAPTTSAPVAAKVEAKPVAQQAPAKVEEPEEDVDMGGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDS0</accession>
    <name>A0A8S1MDS0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0560126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDS0"/>
    <dbReference type="OMA" id="MFQYKEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF137">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="22"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="164"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8076451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="302" mass="34561" checksum="1640FEBD960C6D46" modified="2022-10-12" version="1">MGLSHIFGYLKSLEAKNSLHVAIAYILVAYFFHAVQITFYKSAHFTVVPQSLFLRSFFSLTFIFMFQYKENYHPKNRTRYLIQSQTLGAVGSLFYFYGINFISLSEGAILFATNSIWSQLMVSFMNRERITKSRFLNSIICIIGIVLVVNPTVTVDDPLMHIVGCLCILICSVIQSLGFIIMKKIGPEVPSTITTSYFHIMIIITSSLQQQYIGRFEYFDGYYKYIAGFSLFTMLGQLIQFRAQTLVTYDKLCNYTYSQTLYIIIIDYVIFNKILSLNGIIGGSLILFGVFKQLAEDKKSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEF9</accession>
    <name>A0A8S1MEF9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0590062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEF9"/>
    <dbReference type="OMA" id="IHLPMKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="8"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="23849" checksum="E4DA949D7121EBA9" modified="2022-10-12" version="1">MIYCVSRTYSKSNETQHHKNSSLSTINSKPPETNNNSKHDLYVSNNMMLSMLTVSGQDSNNQSQITSNTQKEDNQFYSYRQSLQKQLSERKIKRNYDLEPKTRENNSRQSINIPEQTEPQQSQRQSSRTVPNLFKTSQLYTQTQKQINPLLFKSSSNEISFGQYKFKKNEILQDANSISNAPEDDAMIHLPMKFQPFQFKQYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEU0</accession>
    <name>A0A8S1MEU0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0590124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEU0"/>
    <dbReference type="OMA" id="FVERCII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF297">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE-RELATED PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8078019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="292" mass="34035" checksum="CB2AEDF223F7696B" modified="2022-10-12" version="1">MIPNHSLKRLFNIDILYHDIIVIGTQECQRSIVTSIFCESKNEWEVKLQNELGKSYIKVQSASLNAMHIIVFAHKSLIPKIRNGKQYTFSQGFMGIVGNKGGIAVSVNIDDKIFLFINSHLESGQNSESKRQTQFSKLETHFQNQVFSNHNHVQYDYLIWTGDFNSRINENISPQTIKTHSDFFGWLGKDQMYLSRHNKLNYQSQFQEGMIFFPPTYKLLQNYNSWSLDQDFRIPGWCDRILYKENKKMTIQSTSQSFKDMTSKLKLQNYDANFDILGSDHRPVFAQFTVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MFP8</accession>
    <name>A0A8S1MFP8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0560181</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MFP8"/>
    <dbReference type="OMA" id="AMNDMVL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000121">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="302"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8076523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="502" mass="58287" checksum="6213FC6CF77BAE3F" modified="2022-10-12" version="1">MIEIRRPLSEKNLGNRSSKELQRPNSSNQSNSQSKQTLQTMKSSIQLQKQSQNFYSKYFSYKDQKENIEIRSNQERNQSTKIKQSPQDYQKLQEKLLYLENKISSIKSHIDNSQRQTKNNLASKFFAQNNKNNQTSTSVQKTVSIDFTNRGQKDQQINQQIKEQRDQTPVQQNKLKSSTLINNNESKIEGTIIKKPSLSTFVTQVKAPLNENKTNFKNLRPESAKASNTIKENFRRKTSQEKNNNTAFLYYISSIIKGYMQIDITKPIELIREHLLQTMQASIFQKSVKITNNFEDKKINLPTTNKKTIVFDLDETLIHCNESIQVPGDVILPIKFPSGEIIEASINIRPYAQQVLQTLKKHFEIIVFTASHSCYANVVIDYLDPNKNIISHRFFRDSCVQTEEGAYIKDLRVIGNRAMNDMVLVDNAAYSFCLQPLNGIPIINYYDNKMDQELLYLQNYLISLKSVRDVRQYNSQNLKLDKLSQFSDPIELLQQLYKEYIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MI77</accession>
    <name>A0A8S1MI77_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0630141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MI77"/>
    <dbReference type="OMA" id="TIQLHNM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="106"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="212"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="308" mass="36582" checksum="A5367588E17A3A9C" modified="2022-10-12" version="1">MTSDKQANKTTGLGSSRIDYQRFNTAKLKSLESIISDKNNKQVLQPLGRKDQIKMLQDYLQEKINEYSQVEKKIELYKKEGDQMNDYVEDLIKEYCLQLVRQGKKENLTDNQIKELQEKKQQLKQETIQLHNMNKVQYFELEVQQGLSLKLNAQKQQLKKKIQLFEEYYKKTEQKIRQKQPNLDFNEMPKAQRNKLKPNAVAIIQKTPDEILQEKTLLIDEYTKKIEQLTFQIQEITRTCILEKQELIKKIQDTKEEQLKLSDKILNLKLRLNKFDKLEQSLTSDKCISDIKSNDTEHSQQKQQELII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MMN5</accession>
    <name>A0A8S1MMN5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0590132</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MMN5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8078035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="163" mass="19307" checksum="B6E2445567B96273" modified="2022-10-12" version="1">MNNKLADLISNYLNEQKHLTDKYTKLFNQIKQSDTYYGRNSNSILNLKKRSSNSDYSYSSNKEKIHCQFCGKQYTSRLPLKNHIEKFHNQENYPYRQELSTKQITPYDDNDNYENDDNSYNEADEELRQKLLRNVQAIQMKECQTTTHQEDVIISGIDSDSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNN2</accession>
    <name>A0A8S1MNN2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0670073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNN2"/>
    <dbReference type="OMA" id="HDEVQYR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="109"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="245"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="302"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8082078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="356" mass="42429" checksum="5F50D45B238CDD04" modified="2022-10-12" version="1">MQRQPLSQIDYNQDNRGVTPKNTQFKTTTLNSDFKSNQNYNVQFKVKPQIQEQHNSIIMSPAPLRRNTIQQLITSPLQKNYSQKHIEIHSPRYRGISQQPHTNHLLTEVQFIKDQNQKLQESIFKLESQIQRGNSTQFIRELENKIIMLQTLNDKLNQDNSSLLRITDIEGLRKEKEQQQTELLQMQEEYDRLLQKLEQFNCDNLKGLDSLNEYNKLKSLVSDLESQITGLIIENEQLNIIYSEFSNLDQEVQQLQLELQSQQIQYQKSKHDEVQYRYKYDSAMHEDKMQKQKLNSQRNTHLDKFNDKIKLLEEENKKLKNLITDIDYDEEIKELEDRISIIGQNNRKLEHQLLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPC7</accession>
    <name>A0A8S1MPC7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0670079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPC7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="80" mass="9795" checksum="FE3B8F681DA0999E" modified="2022-10-12" version="1">MKKIKILQRMGNQLTQNQQFSNRIHIYRNMMGIYKDEEDDGNEEEENQMIKIQRRKCMLDSSNFFLLQLKLDNTDLLEYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MT46</accession>
    <name>A0A8S1MT46_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0650179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MT46"/>
    <dbReference type="OMA" id="TIENKPC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8081351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="26800" checksum="BD9DAB8B02114A1E" modified="2022-10-12" version="1">MQECLKFIYKQDKTKNDLMAVSQALKKWPQEAQQFRQYCLNSKIFVSNEVPKHVYVVTKGEFVQVKYVKSQSKNPLSYISKIFAQEPQIQKQLRTFKVMDVIGMDEIHNDNYTYDVFCNSTIGEVFCIKKCVFEKIIPNQTMKKKNLQITIENKPCQMIEIHSAKGATPINRLQLGEIPRQPEEISTHRHEKRTIDFTAFKQIKPQHVVHQPIAEELEEPPLNNRFLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTI2</accession>
    <name>A0A8S1MTI2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E3 UFM1-protein ligase 1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0630079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTI2"/>
    <dbReference type="OMA" id="ECHEVNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061666">
        <property type="term" value="F:UFM1 ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990592">
        <property type="term" value="P:protein K69-linked ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032434">
        <property type="term" value="P:regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018611">
        <property type="entry name" value="Ufl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056579">
        <property type="entry name" value="Ufl1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31057">
        <property type="entry name" value="E3 UFM1-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31057:SF0">
        <property type="entry name" value="E3 UFM1-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09743">
        <property type="entry name" value="E3_UFM1_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="E3 UFM1-protein ligase 1-like N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8080075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="715" mass="83258" checksum="F3AD5D0C26B84AAB" modified="2022-10-12" version="1">MEELLALQQQLAEIQETSTKYRLSDRIIIDLIEKLVKEYDLKVYHSTDGQSLVTPNQLSKEIHQLILSNKRVAVNQLPEILGIGFDKIEGQIEKDSNGWDVVRMQDEMMSHDYILNVCEEINEDLQQKSIISLQEIMQKYAFPKQFITKYILKQVGSVIQGVCNEDKLTTVTYQEIITARLCGILRATLSPVSFLKLNKDLEIQNSQKICEQLLLTKQIDGKIISGQYISSRFLQNQEAQVKSFFEQNSYVEYDKLYSQFFIQKPKEYLKQMFKDNVIFLDTCGFSKDALISKQDQIQELLINEGHTNLQEILPVILSDQDIETLFSLMQLNNCEYANFMIFHKPFLDNLALAFKDKIIESIYQNPQKLIEQQQNQDDTPQDIQASAGGFTNKKNKKQQQPQKKKQQNKQELFTNKEITDLLTQKKLVEYNDCVDELFQFLQPRLSTLYEQIKIELFESKKSASSQVIQEIQKKIEDMALGLLITQRSLQKIQQECHEVNTKVLVDNCLFGYRLLVENLVVITCKKYNIQLPQNLFVDPNHPSVLNGQINFGLQKTGRVFLNKVALGEAIAFLPKEQQKALKDTMELYTKKSLDVLSQHQFFDVFNLKVHLDKKNDRNLLLFIKHHCKEYIKQRQNEIFDENIFNQIILAMSTDLGLYFVGNYDQQFYSALKQVVYELQNEKQIKTLLNNIINSLNQQLEITAETKELLELLKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MUC0</accession>
    <name>A0A8S1MUC0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MUC0"/>
    <dbReference type="OMA" id="SEMECPQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="263" mass="30392" checksum="8CF55EDA972D6804" modified="2022-10-12" version="1">MKNHNIPKSSERYGKLILPIRHSLPVGLFSSQNSLFASIISVKNRESNSKSQKQKNSQTHFEAATQRAVTPNNQNPSLPFTTKYHLSSQTQKPKKKSNLINKESFQSKISNLICQNKSADFDNISSQIKDRDDQELNKIFNNLKWISQQINTIKVNQLSQQILSQWQKVLTSNCEKVIEINKQKKKSEMEYPQLIKSNSSSSQIFDASGLNLEKLLAEERRNRLLVEEQTSKIISSQESQIKSYIEKIKQLEQRLIEKNSWQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MVP7</accession>
    <name>A0A8S1MVP7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0720053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MVP7"/>
    <dbReference type="OMA" id="LHTMREM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="459" mass="54251" checksum="4F1215B245F6238B" modified="2022-10-12" version="1">MLFQSNPQVAKNLLGNRQKRFEHTMKSMQKIKTFVDLTKRLKLLNPLQFKQVQIIIQHLKQQAKLRQFFKKEDDEKNHIIAISKQLELEIDMLNNIENDEEKQNLILDILQLDFSPNEIATMVQESSMTLETHHNLHRIQETINKRNLRQQSKNQVLDQINNLNFLQDVNNDSSDFYKETIKSFNSKRHQQQNNELSVNEKFELLKKYLLQKGIQIEKKEKVPHQEYSKFYAQQANKLIKSTKLKQLKQIHVSSPKSASISPELLHPQSYHTNHNQKKMEKLESNYQTSNNVSSTTLNTPQLTPSKLLKQKSEEFLSSQQQQSNTERIIAPKRKSDHQIISSSRNILLNPIENEKEDQPIKINQSSQPQVSYFHKSIDQLREFQANTKAVQTFFRMENKDSLHTMREMEKILKKPLKQHFNEFMSVHQEENDGTDCLGKRKNNMKRNIRLNKIFKSELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N006</accession>
    <name>A0A8S1N006_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0750192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8086028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N006"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="49308" checksum="35930FA9D3EEAE49" modified="2022-10-12" version="1">MREYCLQENTLLSNEARLVLKSPKKMSNHQTSLILKTALLGKIVMYIILIVSGIASLFDNLEILWNLLDILQQLSYMKFPENLQIQLEIYKIGSFTPIISYKQIYIYKIYLIVKIPIIPAKWIYQEYEIIVTFFTLSIFGFAYFQFHFASINPCCYFKLQLTCYFLQQQIRKLIQSCQIYFLLIEIGKKQYQQFTYSGLIRILTSYFYELTFASILQIANYNIDTTLNATIHYQHQLHFNQIQSSQYKVNKKLSGLVEGINVEQGSKQYFTILLIKKTLIIVNLVVLQEYAAAQSLITACLSGLFFCYFYIYKPFENTYENMKIFITEILIMLKVILFSGYAILKFNQNKDPAEVLCLINVKWFTLMLVSPCSFRFTNNYQNILIPLCIYILDGQNFDQLSLKVLLTRIQNFKNTNIKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N413</accession>
    <name>A0A8S1N413_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0720063</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N413"/>
    <dbReference type="OMA" id="FETHEYT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035908529" description="Fatty acid hydroxylase domain-containing protein" evidence="6">
        <location>
            <begin position="22"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="7">
        <location>
            <begin position="129"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8084433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="278" mass="33433" checksum="C45037E8BD0DDE8A" modified="2022-10-12" version="1">MIKFRQIFLIILAFVIPVVRGQMEDISFGEKHIYINTFQQYIEKIIFGSIGGIVQYLICCSIVEYYNPMPKNKLRLQNIFREIKYGIVQLIFGCFVSMSFYYYLYPYTPYYKYFENHDYTVFHFLYGITFHWIWTTCVGYWTHRMLHLKYFYKYIHSVHHSFKEPTAFCYCAAHPLEGFIEGNLLLHTAELILPIHPMQTMIYGGLMAFNDLFAHDGGKYDHSDHYRHHLYYVVNYGDAKIDRFFGTAYNPKNYPVKEKCTYLPDFKDKDHPVFQRYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4M3</accession>
    <name>A0A8S1N4M3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(+)--protein-arginine ADP-ribosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0670012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4M3"/>
    <dbReference type="OMA" id="SCKTITF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51996">
        <property type="entry name" value="TR_MART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="749"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="783"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="814"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="181"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8082004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="977" mass="112191" checksum="43E945DD0F6FB3C9" modified="2022-10-12" version="1">MNLIENDNRSEYPIFIWVDKNIFNWENRINCEQLQNDYQNLFQAFDNISDALKILNKIKDKDVTILTSAQCAYQLVKQTKKNMIVFCGTKFYHLNLFLDNTQIKSIVSNSFQSAHEDVIMSMNQTDIYNDFIKLLSPPQQQAIEYQHSKDHVSIQFLVVIEDIRKNKEPEESDIYNEIEALLNISNKQQKKQNILEDLQKKEIEKEQLLKEKFNLFEKLIYLYSREEIYQVFNQQFAQHNYQKIKNIILCLYQGFKQLKEYKFFSSLFRGIYFDSKEIFETIIQDLQSSQMEGKSLFWNTITSTSLLSEIAYNFCSSCNYGILYQILLDQDVPHPCFKLQAYHTQYPKEEEVLLFPQFEFIVQEINCIQVEPITIYLVTIKQVKNNYAFALDPMLRKIYWDKIKEEKIKPKIETLVNFQSKRIFELISFFAEEFKDQEIYMDEILYRIENELVNVFGQIQMQLTKILSHSNYTDVLSQIEKLTKTYTQVIKIEYQNQNPQQFFQSIEELIDKVIKSLRQNICTLILKGIINIEYWKEYLENLQAKLKMKQSQNSTIAVPNSQKVDPSSLQSQPVYKGNTSLLSYQQLKGQKITSLQQVQHKNGNFAYEATLQDGNKLLMHHNPNNNNELTFTKKIDATGKNGYSNKWQKVGKPLETKSITVQEVQQSIQGQSEKFNMQSIQAKEMNYQIQQAKISGQIGGVIAGTLGSLTVDCIIDGIDAQKLGKGLLASATIQGGLIYAQSVQSFGRAVPYVGIGLTAFMTSISVGGVILSDFLSQSEKVYNSMLIAVKTGSAIGLGYFGIEGGMALGATSGPIGVVIGGIIGGFIGGASGNLLGRAIDHFTQFTLQVDFNKNHKSKVEDGYLINPGQLPKINWKYVKDKVKSLILIAQTDTHMACLIPNIDRNRTEIQENEDIGIQFNEYKYIGPDDSCKSITFRLLATTEEYVNDDEILQQLQNNETNIIDIAQVKINLENIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCM4</accession>
    <name>A0A8S1NCM4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lsm14-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0860051</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCM4"/>
    <dbReference type="OMA" id="NRNNKMM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01736">
        <property type="entry name" value="LSm14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025762">
        <property type="entry name" value="DFDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025761">
        <property type="entry name" value="FFD_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025609">
        <property type="entry name" value="Lsm14-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025768">
        <property type="entry name" value="TFG_box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586:SF26">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_51, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586">
        <property type="entry name" value="SCD6 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12701">
        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01271">
        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51512">
        <property type="entry name" value="DFDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51513">
        <property type="entry name" value="FFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51536">
        <property type="entry name" value="TFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="7">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="DFDF" evidence="4">
        <location>
            <begin position="127"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="FFD box profile" evidence="5">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="TFG box profile" evidence="6">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="153"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="FFD box" evidence="1">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="TFG box" evidence="2">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="205"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="256"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8090470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="32268" checksum="34D23AB5DBE1304A" modified="2022-10-12" version="1">MAQVFNSKVSIITKSEIRYEGTIYQINPQQQTIALKDVRSFGTEGRRPDHEIPPNQQSYDILVFKAAEIKGFKTLEENKSEDLKFENVKQENQSQQPQNLQPPQNIEQIEQQQEVRGQFQNNFPQNQQIKSNGTNPRAFDFDEMLQKANEIEKIKKQEGKPKYNPTSFFDSLSTSTQKQDRQTQQRNQNQIDTDTFGNFYKQRQHNNNNNNNGQRRNNNNNNYNNKNNNNNNNNYYRRKNNDPQRVVYVEKQSLEQYQQQQPQSQQQQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEK7</accession>
    <name>A0A8S1NEK7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0870124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEK7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="55" mass="6499" checksum="02554607C04A0472" modified="2022-10-12" version="1">MLISQSLHQLNKDKEQRDYNQNLNKRTSQELMKGQTNSKNCQLKPSFFQMSPLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFF5</accession>
    <name>A0A8S1NFF5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFF5"/>
    <dbReference type="OMA" id="NTWEILI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="58301" checksum="9B1E96FF9274BC08" modified="2022-10-12" version="1">MNKYTLRFKDQEIEKKYQNHKVKDIQFPTLTYLTLGGTPLLLIYAITNLINIDYTNALINGIFFVYMLMQYLVLRKNEYIRVNYCDLALMLTNFGSLYYEISPKQYDYYNGYIMGSNQMLIHTLLMFACNLEIGVFSNLVLTVVRVILAVENSENMSLLQYLQTVIMACSFIIVQYQIEKQFRDSYLLSLKDNTWEILIPQLLNKPFFIFAFNQEVNMYQAIMSNQNQFFNSDSPLTTFLFQSKVKKKSLEAYLVKKSNQKGNFSKLPCLFNQQVNLEYQLKKLQVSVIGCKFEKIIYAVIIDSEDPLLRESKIKFNQSLQQCKDILNAQILQINKVLTLYLSETYSSLIRNLRISLYEIYYKQVDHKKVQLVKMKKLLIKCQRIFETPTNKIKIDCQEQIKFATIKSTLIIFIFEILKLIEGNQQVFLLVNQNITTKLFVYGLKDLPRSALFEESKKVLVEQTMYTAQYYSFEFMRSPQSTFNYENQIKQESIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NG05</accession>
    <name>A0A8S1NG05_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0830092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NG05"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="76"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="121"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="356" mass="41685" checksum="F999C05418C203C7" modified="2022-10-12" version="1">MNQFYSQSFQEKILNSRNISNYQLITSLPTLNTIIYYDFLIAFAINRNNTHLVAGANQKIIVYQLIDGRIKQINVLLNHLNYITTLNFFRQNQIFISGSRDSYFILWSLNQISNGKYFLKVKSHSAEINCVALPISGENFIITGSSDCTIKIWQSQNNSFSQLQCVQEIKEHSNEVFGLSINCYGNLFVSCSKDNFILVMEKSNTKNWILKQKIQILGFGYRICFITDYIFAFQPRKPEPHNMLQIYQLSLNLNQFILIKECLIEGGDQSCNNHFQLLYNPNQQILFNKNGFYLNQIIFHKEEKQHNQFSLIQAINYETDTFYGTMSQDGQYLITWDTKSIQLQIRKYMNKKQINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHE4</accession>
    <name>A0A8S1NHE4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0810007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NHE4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="149" mass="18036" checksum="814D082A565A4868" modified="2022-10-12" version="1">MQYSILGVHLILIEIIKAYDEEYDIEIKSITPILIEFADTTHKIVVNFISQYLQSCFRRQNHQHLKQHSVIKRSKIGMQISLKMSEEQQQIMKKTSIVRRSLFYVIIILSRISFYVEIKKKWNKLEMFSQLYDMKFHNIEYLESFEYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJI7</accession>
    <name>A0A8S1NJI7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0850219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJI7"/>
    <dbReference type="OMA" id="TINLEMN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="59970" checksum="CD0FCEDC4C57CD8C" modified="2022-10-12" version="1">MNRLIQNIRKIDFFSVTYSPAISDGFNYNHSSVLGGMISIFIAILSLMYGIYYIYIWWTYQLLPKVTSDLNQFSENDNFGQLMNYINVKPAYQQGWSPINPFKRDEIILQPLIIDLDDIEFKWKPLLSNQQINDVLTINLEMNYTKQYYIIFTLCKEDYLIDQQKCASEEAKNLYLQQYGNSLYVEIQFTTINPSTFEPQIFERTYPINIHSQAEMCSSIILHYQMNHYLIDRAFLFSTGQEEHNFISNSLNYPQYSLKDYCDKQFLPNTYGAFFVLFQQQYYTVQIAYPPISEVFAAIGSIISILFSVKFIITILNLQQLRQDVLDDIMTQYYPEIKQFQIIKNWFGKITQVKFKGLPVDISSYFQFQKQIHFQMACKLNYKNLLYEMSRFQFILLSMKRRTEIERVHNIGIKVPIQLSESGDSYTITQGQVNQLKTINLRNNNQSEYKYDILTINDALILSYDYKNFEQSILSSTKRSANENNQIEQDFFDINQIEPQYNQNEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NNA6</accession>
    <name>A0A8S1NNA6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UvrD-like helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0890095</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="RRSIDCY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039904">
        <property type="entry name" value="TRANK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21529">
        <property type="entry name" value="MAMMARY TURMOR VIRUS RECEPTOR HOMOLOG 1, 2 MTVR1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21529:SF4">
        <property type="entry name" value="TPR AND ANKYRIN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="6">
        <location>
            <begin position="795"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="416"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="544"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8091856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2336" mass="277034" checksum="072F632FA6FEE6FF" modified="2022-10-12" version="1">MNNQVFKSEKEYLKAKQKLYQDKLNLILTDIDQFYRIRLSGKQFLIRYFQYLMEVNERQQRGDNSPFFYEMANYFREFNIKQFFPKKIWQFNVTKPFMNSLSNVSETDIINIFILLNLILEGKFNYYSESIIKVLPYEGGNVKFPFEFNYIIGFNFQGFLKLVIQVIPKLYPILNDEGKPLHFEFRHEIILVGLGPENQKNSIGYYEIDKYYRNQQTSPNFFANMKLVKEKINTSQIIPHATKIGIKPKSYKDILLPREFQIVKLDCQDYVYNESFKRWIPASTQILIDNKLARINQFSNNHFLIQKYSQQPSAFQLFKIIQNIPNYKVNLTNEQQEIISYNGDGLVIGRSGTGKTTCALLRLFTTDILFKIRSKLDQINYQTTDIQLNQQQKDCQLKTVFVTASPLLACQVKRLYDKLVQNIQDVINNKKRQQKNQMETQIENDQEDLDQSTYQIIDAIKSDQQQQEYQQLNQEEEMDDQEIDEYEKEMGKFLKFSDVTQFPIFVTLRKFLFLVDASLINSFFTIFGNKQDRSSQWHNEQFGQMKLSQNNEVIEKKLEQFNQKLMDEIDNQDYIQIKMPEVTQDVFVRIFWPKILTTIKSKCPISKCFDPIYVWSEICTKIKGHETSHEYPDRYMNYDNYYYMQSLPEDQCSFVYEAFQIYEQHKYQLGYYDLLDLVNHINYELAYGNDIIENVHYLILDELQDVPKAVLILLDQLTELGLFCCGDNAQNIAKGISYKFFEVQNCLLSFRNSKKKIHRNLKIFDLSVNFRSHNKILQLANSVIRMIEIFFPYKIDKLKKETSNLKGPKPVVIKSDNVKHLLNNLCDFFSNDKQIVEFGCNQVIIVKDQDSKSKLPIELQNILCLTIYEAKGLEFDDVILFNFFHDSTASIKEWESLNDLEPQVEYLKKIDYEKYITNHQTEVIASSKQKNNELVEVWQLKMKNMKECQEISIDLCQELKQLYVAITRPKKRLIIYDQSLEKRRFIQNIWIKLNAVKIVDCQIQPQDIRFQLLLQNNNKENWKRQGFKMFRMNNYDQAAKCFKFAEEKQLEQKSIAYNIVVNNAHISNSHQQFLQAAIIFEEIGLLPRAAQCYFTAKQFQKAQELYEKLGQINEMAESAYFAHNYQKAAQGFEILGDIRRSIDCYCLQKDWDNVIIMLNKYKQEFSQKERLAFLNQFFPNYLQNLAQQIQDKAIEQSDCTQEFQAINQEDKSILNQSESFMIQNSIINKSEEDYQVINNQIINQSNNEQSLEILNNEEQSQSFQIENESQQNMEHLSIFDPEDEWLKYDQKQLIKSMSSKISHQSEFSNIVLLNQPPQITLLTSKNNIFISNQILQELIHKLQLFSNEFKNHLDQQKYKSVQLSNRKDDEKEFDHIINFVYDLDNIDIDSIYMILDILEQFKSYKLCIYLCNHFKLATYLGRYLVSLMSTYSTLNKTKSQVNIQMITFGSIRRNLLEQSAISQIAVNNILETINPIFLTFKNEEELSLNNSLGLNCYQQLIGLGFWKSFIYQLNFKHSIDLCMSFNNYSDAIEIFKKVQLNRKYELSEQEKFKLMKIQYYQQIKLIQGQQERASDFVKELDDIFQITKNYYMNKKQELEEKDLNQLIINANIHKTNLNFNQKLKQIEAVILSFDILKLRFECSYEIFIGLFEIVEYFHNSLITFQNQEDIKEALLICYGFSIPQGDIMIHYSHSLLIHISSKIVKQVVKDQKNKDDIQKQNSSLIFVDIGYEYISIQFEQAIELIKNSFSQKIMMILNNKSKKSMDYYQVNDDDSFSVNSQEDWEDTLAELLVFLMLEQSNKKFQCKIAQKKEEQKKLHPLSIYYSKQTELQEYSIVDSEPSQILQLIQRIILKNNLRIINTPNYIKKLIRDISLNQIIQIKNQKDNSHSKMIMAINLLNLTDQLSYAVLILQNSKKGNEPYLKYIEFLECQHFGIVEDVIGCFLDYSQFVNDKFYLDEQLYHVIRIGLNILISLLCNSQEKLTIAKSYKEYLNNIENVDENNCKLETGIFQLTNQQLIEEYLQFLFDFYQYCCSQYYQDQIQQFLMIFAINLLNIQQGKIIKSLIDYIKDNQKLKNLQRLQDYLIKEISKNKQEVQKKKFIIITLTSYLEEDIIEVKIVNTKSDQQLDECYDKCIQVWNAYQSETDSKINCMKDLIKKWKGFRQNHKIYLTEQIKMNLQFIRSLEYFKIGDCQNLRDLNRYMRQNISNFNEDLQLSVTLQRQLLKARQNFVNSLDIHLINSLLNDLGEQMKLLLKGNDIHQKVKQLEMDLKMWEKNDEERQTNEKMLELNKQLIIEKWKDLKAGIKLNSNKLNQQSNSRVQKDQLTTIQEVVEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPH9</accession>
    <name>A0A8S1NPH9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0950031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="SIMMSTA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035753116" evidence="1">
        <location>
            <begin position="16"/>
            <end position="1433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1433" mass="162617" checksum="2D4143310B2987C9" modified="2022-10-12" version="1">MTSKILILIVIAVAAKEQLKLQPCDSKVSTLKVFPSTGETLEWDLKDTFFEGAYLSYSLSPAQTLFQIQKPFAQLSSPKPHFESINRVTASRALTEKGQRVWMNHFVFIEVELDSIDLFYAEGAQGDNATPPQFNKKISITTGSSYLSCLGVDYLNKDQFILDCVDSQKLPFTNKFFIVDKNGNSRIHQNGNYNNYKVNTKRISQTVVFTNKLGHEHTFLFRSTPAYATQTTGLDQSSLLEVFYIESDGTPRLQSVLDAPTLGVITDVQNPEKFIFQLIDFKVFPNGLIYILTAKDGIYVLEFQGNGEFGFIERIVTSKDQAYEFDVDYLLQDDGTVKEVIAILYNDYIQVVEGKVPINNYILEFTASYPASLVISQQFIIIQQKGVTYLFSTEYEDFIYKEVIEGAKGYIINPYETELISITQVDSRRYSLTHGLLRFPSTQNTTSTLQSFTLIATDTFSQQCKVTINYKVYNASDYSIQPTDELQIPTLIYDIPEPFNIDPLASGPNLNYLQLLSQKESTTVNAKITQLQEMLLESISLPNPSQVIYSDVLILDSQEESYALLLQTSDKSITIFQCHIREKLKARSVCQQYQKFQSSTLLNMNNFIWWYQGFNIYHLALDNDYQATIRVSGVNGTNVVDTIQYPAETLNKITSVTVLKDRFYFVLGNKKQIDIRSTYSPFELEYSLTEVMLRYRGFTGSWNPQRIYGNRVHNSYLMFIANDDNVIVADYHAEFTIIKVITLSKGSIDVAVGLESFFIVLKSTTQNLIQEYDLQNLNSIHLMKNVPLYWYTLQTPLQTDFCSDTGVLFVRAYDSKTAQTVVLVYGPGKLLRDSLLKVIPLGSRIADGTSIMMSTAGSDQMVFYVNSGTLQKVYSYFNYPVLTIQPNYDSSAYFSVYTIKFQVTNYNDITPITYYQVINLVNTQITLRIEQNVLDYRPLNATNSEQNIDLVADWFDGQAVSLDIDCNQCDSYVKPIQTVQQMDSGSFNGYHIIDAAPYKGSRTIYLANQETLRVLIADSENNEETSYSISLSTTQSSTCDHIAATEDGVYIFATCQLNNNKVFHVAKCSTTGLLECHQFGTIQTIDALFTVSQLAVNGNYVFVLNGNPQRPRNYEGSLNVYRWTASAQQVTFDLQKIFDKAFFGNQSSYFSSFYLANYKVGVTNYLKVLLVDSKQNLYAIDASLSTFGVITWYTTYSQLNLYTFINQGQFVSQTSSYYQVLELAAPYYQSSDQTIRLRVLITTDSSAHYAISLTFDGKSISTSIPLSYQNVIYLLNQYSTWTALNKAQVGNDYAVIAYTDNSQLLLASYLLPKDPIQGVPLIKLVGGTQSKYGYPQVSDFLAYIPKNIGFPRLYANIIRNVDIKENLLQTYYLNPTYQLYVYNVGGDLAEQYVSLTLNNAFTSDTGYVLLRQFKTEQQEEDKSLLETVYDIFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZI7</accession>
    <name>A0A8S1NZI7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1030046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZI7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8097089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="18829" checksum="047F884F8C0ECD0B" modified="2022-10-12" version="1">MLDQIQNQKIASQSIIIFGEQQISLKNILENQKTGYQQYIVRRRRLILILQKDQFFFKCYYEFKKRFISIRVQIITCIFDNINPRCKSIVLQKGFLLRIFFRTQNFVSLLVLMKSIYFMRQNYIFYLYINQNIAQDLYICQGIIFEVIMVFNKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P258</accession>
    <name>A0A8S1P258_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1030159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P258"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8097293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="135" mass="16969" checksum="9A2803F27DE8503C" modified="2022-10-12" version="1">MFNLKYYFKYVDKSIYKIEVYLINDTICFIIYLLIYIYYAIKKYSNRVQLNDLESFESRFYMRFYDLNDVDQKSLQKFIEQRFVNRTKIIRREEPSRNHQEIKIMKMLQFAEKNYYKINNQQDWNAICTHIFHKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P4F8</accession>
    <name>A0A8S1P4F8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1050019</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P4F8"/>
    <dbReference type="OMA" id="NQMEQWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="8"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="8">
        <location>
            <begin position="95"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="445"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="420"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="445"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8097754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="618" mass="72681" checksum="EC934A66B53A7C94" modified="2022-10-12" version="1">MNSELFACQFCKEYFSIQREPFLLPDCGHSVCAECLNVKLKNGNQFVCKEDGVQVTRNQMNEFPKNFALLQIIKNRPTNRKIVMNNSPKQLENEQDSDRCKKHGEKMDVVCVDHRVRICAKCALFGDHADHKVVNLEDALKKIIRRIDELKDMSERLDKVTNETFEMSQYFNKIELHFQSNLDVQIKQVNSFFDELTLVLDNKRNKIISECKEKIIHSQELYNKYITEAFIDVQRKVELWRMTSKDRIHYYEEQQKVNSIPFELISISSTNELMLIGSQYLKELESTKQQILLKIDEPSYREVHLELKSDLEQFINTYLIVYQKDPQFKLENSLSRLESITECSLLKDINSSIIQDPLNQNQMELWIKQYQENQQQKQLNQQYLYQQQQQEQQQQFLYTQQQQLQQQQQSQNQPYQQQLSYSNHQRTDQPLTSQELDDQYLNNLKKSPSATTLATRRTVSPSPIRRSIKQKKINEKFQPLIVKLKGDSLDQCDFSQAELGDEGLLSLITIIKKTKNLRVLKLAKNKIQDGAAQQLLIELIEKQNENDQENNQISTINLSSNMLTDKIIDTILDLCKKYSKNMQHQCLNQIYLNQNIINLSRVKKKVDEIKKYGLIIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6L0</accession>
    <name>A0A8S1P6L0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA primase</fullName>
            <ecNumber evidence="9">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1070190</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="9"/>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6L0"/>
    <dbReference type="OMA" id="NVTRGFN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00335">
        <property type="entry name" value="primase_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3 9" id="KW-0639">Primosome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8098737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="410" mass="48233" checksum="2B283EAB810B6FCC" modified="2022-10-12" version="1">MNDQEQKDFERNLYAYYKHLFPIQMIVEWLTYAKDNPPEQKYFNRREFSFERSLGGDTFYQRYQQFQNAYGLQKFLLDNQQNDETSSLIKYDIGAVFDKLPKKGEKLKALEKEIVIDIDMDAYDEVRTCCQGAKICEKCWKFLQVACEILRPALFYDFGLEHILAVYSGRRGIHLWVCDSHIRKSTNEVRSAIIGYLNINIGNADSEEIIKEIKQEKIEKLHPSIQRAYMIAQQFFKQIILVDQNSLKIENVKKKILTILKQTKILNPKLEKLILETNDSFQIWEIICSQDSERLEIAKLQIILMCLYPRLDAHVSTTINHLLKGPFSIHPKTQRVCVPFNLKDSSQFNPLDTPTLPNIIQQLAELPNQLKQRENENKVFPFLGKEIQSLKKYIELFEDFISKYNKPKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PAU7</accession>
    <name>A0A8S1PAU7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1110185</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8100210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PAU7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF47">
        <property type="entry name" value="TRANSMEMBRANE BAX INHIBITOR MOTIF CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8100210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="35099" checksum="1A84F0DB28A9989D" modified="2022-10-12" version="1">MNNNINNYQVNQNDQPQYLNLQSFETQDGKTQKINQFQDQGIFQDLQIRVVFLKNLYNILFFEFLYNLLMFGFGIYTRMATWLLKFQDFYCFYDEETYVEHCFFDIEPSWLFYVSLFISIILQFILYFKGDLVRNAPVNYIVSIFQIVFFGFTFTTINIFWALKLLPIAVWITWGITYLIIISLILYAFLQKRDISWQVGTVVILGISLPLIFIWMLTYHYYPLWAKFVCLIVILSYGFYLVFETRLILRKERFNLDTNDYLIGSLLLNGLMLQPLVRIFEILFNAYGRRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PDW4</accession>
    <name>A0A8S1PDW4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1140132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8101144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PDW4"/>
    <dbReference type="OMA" id="FMPTKQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="363" mass="42619" checksum="22DFEFAC05FABA51" modified="2022-10-12" version="1">MALIHNQNDLQNMFSKAGKITSEKYIVFQHRDDHPVKKFKTPQSIKSPDLTKKKVHSKQESLNEAEQNHLNHLLKLSQPFSKQISRQIGHYTQIRNNCKASCGQYHIKYSELDKAIHSVPNYDKTLKHTLPIEVPPKYHQDFSIDTPIIDKPKVKNESFVDLQRQIPRTDIFSGRPNPHPDRFQYLNVTNQWSKIPRQPQIKLEKQLSRDQMLIYKKKEFAPDYKPNFEFGKKKLGSCGAPFYKLEQRKDILTKIPPYTFESYFEYDEYSKKPKSQLFSSPTAPNFNTMLERECDQHSMLPSFMQKYTNTRLGITHLNQKMLEVNNFRDGRFLTVTSSFMPTKQQSSKKNLNKSSQNQSIDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGC5</accession>
    <name>A0A8S1PGC5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1180079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGC5"/>
    <dbReference type="OMA" id="CLTNNIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16306:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_7, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16306">
        <property type="entry name" value="TRANSLIN-ASSOCIATED FACTOR X-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="589"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8102390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="675" mass="79734" checksum="F6174AAD57DABF65" modified="2022-10-12" version="1">MDVLQKILKTQGTNVTAIDLEFKQIQNLEELMPQLANFSNLKELNLNGNRLTNLPDDLELLDTVEVLCLTNNIFTDLGQVVEALQTMPNLIQLEISLSSKEEEQFIIESLPNLQILNSQKINQDQEPVDETSEQQSQQQSERSMSAQQEITLQQYDLEQMAILYDNIREYKKDEQDDKLFDQQIRTIMLDLQSKVKQNNPDHLTNLYILTAKYNLYEICFKSILKYFKSNEKKLDLIFSKIHDMHLSIFNDMSNVIQNVKPSSKNTSQLQINQKQLDQSQQQQVDKSVENRLRQELKELQQINNELESENKRYLDLLIKHSKGEKVSLSNNDNNQKQQDTYQTQVYTKNSQQHNNQQYNNQIFQSKQSQQVNSIPQQVNVRNLTLKQLKDVINEIYESKQKFDQKCTDSKLPRETMEQHMYTFLNQKYGLKNLIIEWATSIINSLKKYAAEDNDIAVFGKILRNECDEEFRFVQTQVKNTMQELLKMYLRGKHPLKHQAEIKEMLNQRINGQLYEEEAVDIIKYMYNQEDSELLLDKLKQYYIVPQKPNDRRLTREEQLMQLQEKDKYKLEYQVFQKIILDFQLKSHEKYLKKFIVIFKEMDTDLNGIIDENEFRNLIDVLNFDASDLDIQKYLNIIDPYSHQQITFSQCVTLFSSESVPGSNGQIQILQRISEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSC5</accession>
    <name>A0A8S1PSC5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1280164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSC5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="178"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8105894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="710" mass="84919" checksum="8F26EA05DF9CE683" modified="2022-10-12" version="1">MNDFFENSGIRLISRLIIYIFRGLKQDYGSSGYNYFYLDDKQINIFIVIRDLKDYSQVELIRWLIFHIYILRFLNNNILLKVNPKYQTAKTIFNYGLIVINIRKILFDYYLQVYHRNIQFSIFQINFFLILVNTHKNDYNKQQRGYKQMYNGFFLRVLGYYIFYKLVSVYQVELQKSQFWSIVAFFTIGRKIILLILLLSLLCKKEIIQLMNLYLCYLLQEKENQNKNQRQQVLTDIFDVNFRQYQCFQYFSLLLLIKCYQIYQQFKELLLTQILNQQLIQKMNINELQCLKEYHYSKPIMGVCIDQQCIEDKSFCHECYMNCHRHDKNKLYLFPSLEDWFKQFFMKFECAIQEMTESFSLIENLKKNFNQFKIAQLNLFMKEVVNLEKQIDMLNLNLNSFILQLCQLSQQTQQNQQSQQTQQNQQSQQNQQNQQSQYNQQNQQSQYNQQNQQSQYNQQSQQSQQNQQSQQSQQNQQSHQSRQNHQSLQNRQNQQSQFIIQFNKISYNDLITQNENTNIQDQKRNQIQQTQGNGLANYDTPTQNSLKFLDIQFKIEEYNSHVEITRNGKLVKCLISENIIMGNPMIPKTGITKFAIKLLNPVYYVALGVGKKDMLLQINNQPNLFQQKEHGTYLVNSLGLVFSHNSSTTYKNFSFKENDTIIIELDMGKSKITWTKLNDNSNISVPFQNSFNLYPLLHLNQTVIQVVDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTC6</accession>
    <name>A0A8S1PTC6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1280134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTC6"/>
    <dbReference type="OMA" id="RIFSHNR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="26952" checksum="625C8BAECC39A71B" modified="2022-10-12" version="1">MIRSFHSISFQQISYTPPQNQLRRSSCHCSHCSSSNIKQITLPNQQYEIVMHRKIRLANIIIQNFQPTDKYLRSKQKYKNFRIFSHNRQQNISTDNHKSPSIDQQDQIKDHSQKKRISPIQSHYSNSKQSNFYFLNHLKTQMTRIDNNLNQLIQTSRQQITEPDEPIITSKRTKQAIIKTIKINHPYKSAYSISLNKRFGPPKKMHTNPSNLLARTQQSLSKYALNCIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTN5</accession>
    <name>A0A8S1PTN5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1290008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8105948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTN5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="83" mass="10134" checksum="E0170503A47CA9AC" modified="2022-10-12" version="1">MYLNAESLQKQEKVDNYHCLIRKNHSNQQNLYTFIRVQFCHLCKQKIQDQLKALTQKLNTILSPQKMRLKLKIFKYQMMIFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUU2</accession>
    <name>A0A8S1PUU2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Inhibitor of apoptosis-promoting Bax1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1320109</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8107025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PUU2"/>
    <dbReference type="OMA" id="SIRMGKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF47">
        <property type="entry name" value="TRANSMEMBRANE BAX INHIBITOR MOTIF CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8107025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="34195" checksum="B9B1934A28BFF196" modified="2022-10-12" version="1">MNNTNRYYSDDPNQQLQYNQPVFNQQNPFDNNNRQPEQLANQIGNYNHAAFNNPMNSYDNEYKGLVSSADSTIRAGFIAKVYAIMSFQLAITFLLILASYYFQNVRNAIIDTKTIKYTPFSIICFLIAFIVEIAIFCCRKVARRVPLNYILLIIFTLCFSTIVAAPCIICFELLSNGVELVIIAAAITLAITILLTIYAWRTKTDYSALGHLCFVLSASLLIMCIVGLFVRNIWFQLFICTICVIIYGGYIIFDTQLIIGNHSNYLSIDDYIIAAMLLYIDIVILFLRILEILMIIFGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PWJ2</accession>
    <name>A0A8S1PWJ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Dynein heavy chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PPRIM_AZ9-3.1.T1330150</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FPYDERA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000002">
        <property type="entry name" value="Dynein axonemal heavy chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000003">
        <property type="entry name" value="Dynein heavy chain 12, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000001">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000001">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000001">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000063">
        <property type="entry name" value="dynein heavy chain 6, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000049">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000362">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961:SF8">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 1, AXONEMAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein heavy chain linker" evidence="16">
        <location>
            <begin position="996"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="17">
        <location>
            <begin position="1541"/>
            <end position="1866"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA 5 extension" evidence="21">
        <location>
            <begin position="2031"/>
            <end position="2175"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain 3 AAA+ lid" evidence="22">
        <location>
            <begin position="2400"/>
            <end position="2491"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="19">
        <location>
            <begin position="2556"/>
            <end position="2806"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="18">
        <location>
            <begin position="2820"/>
            <end position="3170"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="20">
        <location>
            <begin position="3199"/>
            <end position="3421"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="15">
        <location>
            <begin position="3665"/>
            <end position="3782"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="23">
        <location>
            <begin position="3814"/>
            <end position="3953"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="24">
        <location>
            <begin position="3961"/>
            <end position="4267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="922"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="2835"/>
            <end position="2883"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3047"/>
            <end position="3116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF17852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF17857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="CAD8107401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="27">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="4271" mass="495925" checksum="6585FC62F05CC0C8" modified="2022-10-12" version="1">[encoded data, 4271 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYJ1</accession>
    <name>A0A8S1PYJ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1340086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYJ1"/>
    <dbReference type="OMA" id="VIWIENN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="508"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="553"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="592"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8107639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="633" mass="75112" checksum="CD7F889F88CDD28E" modified="2022-10-12" version="1">MDSSQKNPQIREVNSDSASKGYSSNKSQFQSTFKYQYPIEKKNEHEQSINSEQSLSSIQEVNPKNDRSQYDEESQMFNYNYDQIINQNQRLESQNISLQAPINQQQIDLQTIQDQEPIQKVSSPVKLQQQQLQQSRSQSQVFQPFQPYQQQQQISASKSRIDRSFDGISVFTNISKQRTTPNSRNKANSFYYDRIGNQVSEIMPPMIDNYLTHVLLQTALPLLIVFILYVINYFLQRAIDQFCYLPEQYCICNGFGAKLWSWARQLIMWKLGLIVYRKQFLCRLAMGSSNLKHKEPVTEYIFLFLFTIIFDFILVIWIENNQDYVYVILVYCIGVVVLLNTFRVKDWNIVFKKGAGVFLYDFALLMLMVFIYYAIPNVVESLQDRQGKISGMQSFLYLYPLFDWIMEIFMDYIYNQTQFAELYIYQLQLIILGMKIGILNIVDIWTFEFWYNILMLGIMRANNSSMFLTIFLRRWIFYLIPDLQFITKMSETKKSLKGKHGAYLEHQFWIVFYYFFYLTNSSVPYIAKKTVFISNCKFDNETWNLTREYYQPIFIWGLLLIAEIAGIMLNKQKKYDNIKYEGGCKFNLIGNIFIRLSGYYFFQIAVMIPFLFKSLNDISQINKHLEIKDTEQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q0V3</accession>
    <name>A0A8S1Q0V3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1380080</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q0V3"/>
    <dbReference type="OMA" id="ADRIHDF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="106"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="537"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="450"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="500"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="537"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="561"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="573"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8108831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="583" mass="68310" checksum="E7DAD77D0B4BAE3D" modified="2022-10-12" version="1">MLEHKRNHSQKIRTVQIRCYDKQVTIDIAMDKSVADLLNKLREHPATPNSQQLVALKTVSNSITMDYMLSVPEERAQLPVHKLKQEVLIGVYRQIVHKQNLSLLDFTFERCIGKGGTSEVYLVRHQVSGRLFALKMIKKQYITDCRRLEQILREKKILSQVLNHSKFIIPLYATFATKDHLCFLMEYSAGGEMFYHLQNYRFTDDEARAYICEVICALEELHQHRVLYRDLKPENILIDLRGHIQLTDFGLSKLDLSEEQLTNSFCGSPEYMPPEIVNRQGYSYPADFYTLGCLLYELLLGLPPHYSQNTEEIFHKIQNEDISFPEDLSTDVISLLQSLLNKNIKERIHDFNTLKKNSWFNNVDWQTIKEKKSTQMPIFIDIYETHIHQEFLKIDVTDLNQKNEHGELSSSEDLFDFFNYVNETYKDIFQLKQKMRVQSDHLIDKKLLQISQSSTKKKNLQLNLNEIEKQFSKTQTQKQSLTPQSIMPLSLLRKSFQQMMKEKPRKSQDNHPTLSTILSERPIIDRNYIQKMLQTLTNKKKPQSPQHNELSWRPPQIKRGSSSSTHLKSSISQRGQSSTRKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q170</accession>
    <name>A0A8S1Q170_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1400044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q170"/>
    <dbReference type="OMA" id="YENVQQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042507">
        <property type="entry name" value="TBC1D19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16110">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16110:SF1">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="313"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="466" mass="55501" checksum="82AEDE730A3F18EA" modified="2022-10-12" version="1">MQQLDNQEILMKLKSSKQFWDIKKKIKKGFNDQQKIGVNDIQKFAQSCEIEDQLTNLISHILKKSKKRETLKEDQIDFLNTSKIKWTELLFKNLDIVHQKTKNQEEDDTNTDSKLYIYDEKNLLAFLQTFKQQQSSALYQNVQFQSNKLLNINIPLKSSQQILDKYQYLLLQEELQDEKINKIRKQIENEHLDIELEKIAQTFVPNGERCLLYLKYFEFDHQETILQQNFTKLQKECLHQLFYDELLEIDGHEISEDTFYFVFQEQILTCLKCFIRDKTIKENIRIQLQNGIKKEHTEFQLPITGFFPFKNISKYVAPLCYLSTKIHIIYALFKEFFCRYFCFLHAISSENNSILSLCMQIEEIISTNSPKLFHHLKVIGCDTLKLIIHQLIYCFVGDMDPPHILTIYDQIIGHDSLEILVILCVGFLNKYKSKLLLAKNYENVQQIFQELREDKFQELVQFAYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5H9</accession>
    <name>A0A8S1Q5H9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1460004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8110916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5H9"/>
    <dbReference type="OMA" id="AQNNETY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="232"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8110916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="273" mass="32267" checksum="A0761A9F17F1E883" modified="2022-10-12" version="1">MHRRQDSNNNRIRISIDHSVEVRNTRTNISPLQSNHVISPQIYKHLKLPSIKQSRSPSARNQKQTTKQVTSLNQQTAELIKKLSNNSIKQKHHRFNTESRRKSSLSQNKLEPQTKLTSKRTRSISNMVSKNSLIVSTQFEEEEDLPKKNKLPTNLPQNQIKIEIVNKVQSNYDHQQNKYAKFKAQNNETYQRSENQELNSLRQSLFSIIENSRVQRHKFYQLGNTDQQLQLLEQLYLQNEKIVRNYVQIRQEAQEIDLKVKSLSNQRKQFKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q975</accession>
    <name>A0A8S1Q975_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">glutathione transferase</fullName>
            <ecNumber evidence="5">2.5.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GST class-pi</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1490021</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Mu family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GST superfamily. Pi family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q975"/>
    <dbReference type="OMA" id="ADFIMYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000020">
        <property type="entry name" value="Glutathione S-transferase P 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050213">
        <property type="entry name" value="GST_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF222">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="9">
        <location>
            <begin position="3"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="10">
        <location>
            <begin position="91"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8111755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="221" mass="25868" checksum="BA297A1C1905D4FD" modified="2022-10-12" version="1">MGDKVVLGYWAIRGLAQPLRFYLEYLGLPYEEKRYYKPEEWFADVTSAPLNQHVLVNLPYIKDGDELVFESQALYVYLAYRANKPELLGQNPKEQVTLASVRGVLQDLFKSLITLFTIPEDQFEAKKDNFFNQEVLWIAEKLSKFLENQKWAVGDNLTYIDFQLFEAEEILRNYKPEVFTTLVGLKKHNENFANLPAIKAYQVSDKMIARPFYPPGMYRWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9G9</accession>
    <name>A0A8S1Q9G9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1470066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9G9"/>
    <dbReference type="OMA" id="AISECAP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035886335" evidence="1">
        <location>
            <begin position="17"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="109" mass="12615" checksum="FC5D1672AF48F900" modified="2022-10-12" version="1">MIKIFLISMLLFASLSKKHQTIDEDNVCLNEKCGVQINECFEDYECFEAISECAPQENPTVQEIKEAEQCVKQKSEAAEQLLECAQKKCSKKELNKKQMKLIKKFMNFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QEK4</accession>
    <name>A0A8S1QEK4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1560086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QEK4"/>
    <dbReference type="OMA" id="IKEIGCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="632" mass="74441" checksum="5CA9F8993A15EF36" modified="2022-10-12" version="1">MTEESNSILTTIKYYKSILKFPSFPKQYSELDETQEQNELFFCNSKQRKEESLRLLKEWAQNRMELFEKNREIVEELANSCDYRIRLSVDFLQIVYKFFNEKYFHEQQYAQFLITKPQPALKQNPLNQQLYSTIGISISTFEEYNLKRLEKIANFAHSIQTEILKAQSFHDLSDMDKQLDLLLSSIITQKKLLQNYITSAGQKLNQIIQQFPQHQPPQRQRKPIFDDQQQQPQQSRTDKILQNISALGTLLVGASSLQDPVEMELNQQMKEQERQIREQQLKEEQSLSNGSELQQEQEAQQQKQEINKEEEQVVKQIQNDQILKVENQVELQDKQIETQDKQIELKENQQDQQQQKSPESLSSAEYYKQQQQLKEQQQFEQNKLRSKNYQPQGQQNQQIIYEDDGRKQNNQPVDLYRLIISFVATQQYANKLLNQLAEKVLQYWKQVIAIESKRAQWAKDSCLIYKKGIAEVHNLQVELPELSIDVDQHYNLRKILPKEIAQFEDGHQEEFIQTLVIKEIGCQAARLLLVKEFKVHVIDKNNELSAILVLTIDKYVGCWLKHEDTIEAVDNPVLHYPIKGMAIQKVNELILDIMPSRHLLFLNIANDRQKSKIKFQTADDMEEFCTISQAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QEQ3</accession>
    <name>A0A8S1QEQ3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1560107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QEQ3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="101" mass="12087" checksum="C383757A98728FD9" modified="2022-10-12" version="1">MQKNFQYCIKAIKQFQEMSADLGGTNILQALIQGIYDENYSKNKYQHMDRISLQRSISWYLKTKDLKLEFIHQILGIIEVSIQSKYQLKLEMEIVNQQRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF28</accession>
    <name>A0A8S1QF28_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1600066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF28"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="106" mass="13050" checksum="BB3268FE1D592F33" modified="2022-10-12" version="1">MQQGEEIDMKELEELITKKCKAREEEVQKQVTQEVTKYKEECEKILQNIQKEHEKIQKQYGQDQENFEKQEKNYKIIRLLQMKLPRNLNKNNKKIFNQKKILKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QGI9</accession>
    <name>A0A8S1QGI9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1610060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QGI9"/>
    <dbReference type="OMA" id="TNRVEMF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="174" mass="20959" checksum="483CE7C7446536B0" modified="2022-10-12" version="1">MSLNNIFKQKQIERLKDQQFHKQQRESKQEEIRKIKSGQFKARQTNRVEMFEENKLNKLREILKEGSYPILPITKHSFRDRYKEKELDGNIILKSQQLGKDRARQTMLDFKQGHLETGEFNNNEPHKNHYKWLKVEDQSNQGNPMRFGKGDRIETERIKRVVDNNSQKIDYLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QGQ4</accession>
    <name>A0A8S1QGQ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1630016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000170">
        <property type="protein sequence ID" value="CAD8115178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8115178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="109" mass="12342" checksum="E1AE5ADBCB7C09A5" modified="2022-10-12" version="1">MKANKFQGLHPSIFISGXVKEGSIRIDGNISVEELLITEEPKPIKIDVQQPLPKLLLKQKDLSQLLTLTNEILKQGKPKLPKVMSSQHSLPQLPKNPRDRTISQLQKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2024-03-27" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNA1</accession>
    <name>A0A8S1QNA1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1930001</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1930006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000202">
        <property type="protein sequence ID" value="CAD8117243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000202">
        <property type="protein sequence ID" value="CAD8117253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8117253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="112" mass="12938" checksum="025C7F2759B78446" modified="2022-10-12" version="1" fragment="single">MNSEQIVVQYKNKVEECLQGFQQDLDCSYNRIDAYLDQLLLDPYPSVPIKNLNLALPIVQISIPSIQPLHNQLIQELKPIINSTIHKQIKLKQQNIDQLQQQLILLQDKGXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QRS7</accession>
    <name>A0A8S1QRS7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2120008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000221">
        <property type="protein sequence ID" value="CAD8117822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QRS7"/>
    <dbReference type="OMA" id="HKHQVYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001434">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="171"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="219"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="51681" checksum="36B6D6AE1CC19FE5" modified="2022-10-12" version="1">MSIKQELNSILEINLKEIEKHLDCSFDKINNFIDQIFILDFPIQKFNNQQQESQLPKVNEFKLIDQSQFQSQIIQELQPLINNQIKHQLQIQLQQNQDHQQQQQQQQLIQSQFNIKSFNYQIINHNSIKQKQEYCCAVAFNKVCSIVAAGCNNLIKIYEFKQGMLKQNQILNDHNNLVSTLNFMKKSNQFISGDNNGFIMIWQNNNNNNNNQWICSQTLKAHNDRINCLIMNNNEDTIISSSNDFTIKFWIKQNEWICQQTITDHKHQVYQLNINEQQNKVISCGRDQLILIIEYSEYNKKWMVIQNMKVDCQGYRLCFINDNLFTFQPKKGNLMHIYEMNSVSKQFIKTKNITVNQGDDGCAFFPQQYIKSKQLLVSKHNQNINLIRKLENDEFKVEQSIQFGSNCLFGQISDDGEYLITWDHSSKEIQIRKYTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QSQ1</accession>
    <name>A0A8S1QSQ1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2610001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000270">
        <property type="protein sequence ID" value="CAD8118768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QSQ1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="114" mass="13182" checksum="28AFF831E22A7C41" modified="2022-10-12" version="1">MNMKKTDQCDEFTQFKSSIDYQSHGCTQTGKTYITQGSFGKTIIPTPVTLTLNCSLIEPSYYFGCALIEKNFTGCSAQCEKQTFLLLKHILTVHNKWNITYKCTQRFWIYQIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QSY3</accession>
    <name>A0A8S1QSY3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2170002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000226">
        <property type="protein sequence ID" value="CAD8117944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QSY3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="48" mass="5570" checksum="BFBA40AFF07DE307" modified="2022-10-12" version="1">MDLTTIKFLGKIKIFFNLISLNRSVEIKSHNNYSFVSFQHLAVIQISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JM52</accession>
    <name>A0A8S1JM52_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JM52"/>
    <dbReference type="OMA" id="NDDMNNS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="177" mass="20202" checksum="54D3E516E0FE47EA" modified="2022-10-12" version="1">MGTSICVKGSQQTQVEIKTISQHSNKLNGPPNLFRPTPIISILITDEQVEKEESLKIELSQQQNERQVDQLFFYTAQQSEEIDSQQQNPWIKKNFAIEAINVSKFKNDDMNNSQNGNLNNSFDQKNSFADTSKQKPILRKRSGQESSNISLSAGSQRSIKKVSFDKKQQVLYTKFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRE9</accession>
    <name>A0A8S1JRE9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0040020</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRE9"/>
    <dbReference type="OMA" id="YFGKEVM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8042806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="517" mass="60650" checksum="A4F4E975710585FE" modified="2022-10-12" version="1">MLPQTQQNLIPKKKIEHYTYLMHEQIGRGYSSKVYKGKDENTGEVVAVKVIDVKAISNEIERQLINQEINALKLIQSVNVIKLHDYYHTNNNTYIITEYCNQGDLGKLIQQQGIIPEVEAFKILRHIINGFKEQIRKGVIHRDIKPTNILIKNSVPKLADYGFSKMIKAPKEKVFYKVGTPIYMSPESYLENKYSEKSDIWSIGVVYYQMLYGQCPWVINSESEFQESVSQLHFNRNVPISEESKDFLRRALQVDENQRLSLSDIDQHPLFLRRNTTALNKMRGALVSNSFNKSITNMNVVRVNQTQQCTRSGSTSHKHQKSNQIKEKIGTIIQHTYQSFHYESQAEEVICNFINQCKFIYRTCQLIDHVKYLQSPRLRERIMGYFGKEVMDIMNKLKEAYEKGVNNIKVINFNHQFVISILNKYSFREVYLKYKEYYGMSLLGDISSLKDIIRELNHQLRINEQNETGILLLDYLTYYYQVIINNNTQQFLDGKPINEITPSHYQEIRVKIYNLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSF3</accession>
    <name>A0A8S1JSF3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSF3"/>
    <dbReference type="OMA" id="CDPEEFT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="685" mass="77807" checksum="B638F05747986195" modified="2022-10-12" version="1">MRQDDKMEEGNTYEEGMQTQSQSQQHAQTSSSSQQTSYQTQTIVTKTITTSHRVQQSEDQGQFDAAEFEMMNGQGNVEGEIYESASAQYKYGGYNLQADHAKLGGGLMLNKYNFSDASLKEKQFNSSAGKVQMACEAAMAAALRRQEEAAAIGGGMASWELQMKAMAELKAQYQLSAKITVKQRSFLMYGYGFKGKDGKPQKCDDEDLSKQKCDDEDLNKLKCDDEEINKNKCDDVEQEKPKQGLNFAVCEAECIEKPKKKKPEDLTNAPQDPEEFSKKKKPEEPEEKPCEPEEFSKKKKPNEPEEKPCDPEEFSKKKKPNGPEENQCEPEEFSKKKKPASEQKIYSPQDLSKSKNTPRIQSPDRYFQPRSEEFIRLMPKSPQQIVYPVSSVRSIIVPKFVACEPVQVAKQPMLTQCLSVRQPLVIQEEPASFVAVQPVQQQLRTVQPQFVQQQAIIQPVIQQQQVVQVQEMQPLQQVMMVQPTQKIETYSQQQIQVQQPHFISQNVQVTGQQPQYATTQTVQMMTQQPNLVVAQPQQVASVAQPVVLMQGIQQEMISSVAVQQQIVTNTHLIQQDALSPIRQQQIIQQPQVVLQPSQQQQTMYVQTQPILQQNVQMQQSVLQIQPQQTIQQVQQIPQVTSPLRQQSLVLQPQLQYNTQPLELLQRSQSPVGQQQFKFQTVLHKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXG1</accession>
    <name>A0A8S1JXG1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0040116</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXG1"/>
    <dbReference type="OMA" id="CTIENQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="556"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="727"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="758"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="810"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="833"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="862"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="887"/>
            <end position="909"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8042898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="941" mass="110067" checksum="B0B800FFF6458626" modified="2022-10-12" version="1">MQKIIKNTFDLFISFLIYILKQYKYLIYQGYILLNNFCYINDLRIENNLIIEQLDIFKFETYCLTNLGYYLPLVFNQCIPICGDGIITQDEECDDKLTYQSHICFNCKFQCPINCLQCLYGQCIQCIEGFSIQENRCYPKCRDQLIKSDKICEDDNNIQFDGCYKCQNSCQIECLVCQNTKCVQCISGWNLIQGKCNQICGDGQVAIISEEQCDDPQNLNCRNCQIFICNEYCKLCDNQQQCIICNDTFNLVNNKCINICGDGLLTQGFEECDDGNQIEFDGCYNCKYSCSKGCLVCQENNICLQCNNEEQFILDHTTHKCFTQDYQDEEVQLINKEDSSNTEEIQIIKNEDTNNNDDYIQILQSDQQQFELNCGNGKLQTSLNEECDDGNLLGGDGCSESCFNELSFKCQSLENQKSECAYIQAPDFHLNVITNSQNSLQIIDLTFSQQIKLNQNNTIESIADFQIKPETKYQLTIIPFSNLTSELNMSHYQFYIQFEQSVDHPIFTLRFMDESIINSENITIKSVQKSIQIGNPFVLSNSTKKKLTQIVSMNDAIMYTMISISALSFLIGNPEMFFNLLNLLQSFSYIRYVQYQFPPHLSQFLETYSKISLKLALDYLKVDQFLAQLNRGNYPQSKNTTLQTNQINQINQIFLVNAKSCYITVLTSYLTYLMYKIITSQQIDYFLNKLTQLKYENIKYLQVIGLFQRKIQQKCLKMKFEYFSKTIFQVYLTILHQFLFSTFMQFPNYSFESFFEALNSINSLFAIGLIIFINFKSLSITSQQIKNVNKWKFFFEESKSQFWASNYKSFQLYRVTIYIFLIVFFMNQPEIQTILLSLQSLIYLLYLYKVQPLKSQSEYIKLILREFIFLTTVGSFLIYSFNLTEEQYLICGWIHISMLSTILGINLFIDMIEFLGKLYANYQKKKLKLERKQQKNIQFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2H8</accession>
    <name>A0A8S1K2H8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2H8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="196" mass="23702" checksum="925F3DFF7D691BE6" modified="2022-10-12" version="1">MNSTSHYQDFAYVNLLQQQKQQTLLSISQNNNNKINNNNNILKKLIINKNHLKFQLIQKQNLLLLQSILINYLIKLRRREIRITKKKEKFNNNRNMENHDQVQKLVKIEHNLNQNRIIQQQIKLNQINLITHQFSIMLQQLILEQILQYYLIFSSQCCSKTLLLYDIFQFQSILRINLRNESLFDRFLSLDFLIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2V6</accession>
    <name>A0A8S1K2V6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0100472</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2V6"/>
    <dbReference type="OMA" id="FYEPITC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042234">
        <property type="entry name" value="WDFY1/WDFY2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189">
        <property type="entry name" value="LD41958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189:SF1">
        <property type="entry name" value="LD41958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="422"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="486"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8046576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="506" mass="58803" checksum="656CC5CDF2C31482" modified="2022-10-12" version="1">MLLMNESINVTIPSYQQESSNVQYLIDIKTRNKQQWQISKRYSQFEDLHKKLIPLFQELPELPKKAFITFLVGKSKEQLEERRAGLEKYLQQLIVRREIYHSQLLRDFLQLEQCEEILPPNLLHSFKTIMGIRDISLSDQGVMFLLQADMSVLNRVDAYVNNMKMPWDSEQGEVKTIPVGQVECYIKQEEGEFCYKKLWTKEYSTQAICMFYEPITCTLLIGLDSGNINFIKVSERDSFEKYEQSIEIEIHMSRIMGLFYRNGQIHSVSKDTHYKVIDMEQGGLKSDFSIGKHELTSLTYNEQRQISVVGNRNGQIYILNIKPAQPIVMLQIETSTSFIRGLVLDYQKNYLISVSYDDGVICVLDVGKCLQDNVGKIKTKIQAKIKTREVQWSSKRGELFVGNDDGTVTIYNALDLQPQFVLKAHDKAITKLIWQESTQILITGGKDEIIKWWHFPKKWKAGDQDEQVKSKQLIEAKMENILKLQNQNQKIDSDEEDETGLGNWHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4R7</accession>
    <name>A0A8S1K4R7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0110352</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4R7"/>
    <dbReference type="OMA" id="GWITDEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336:SF3">
        <property type="entry name" value="GUANYLATE CYCLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336">
        <property type="entry name" value="OXYGEN SENSOR HISTIDINE KINASE RESPONSE REGULATOR DEVS/DOSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="7">
        <location>
            <begin position="734"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="85"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="396"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="396"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="429"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="442"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8047286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1080" mass="123493" checksum="05D1EAB4E99081AE" modified="2022-10-12" version="1">MMDSDRMSERYTNIVNNNLTFRDTEQNFLPLQVVDNFNKQLAEVKQEPIEQQVVDQPINSELQRGNTAQLFTDKPLCLPIVQKKLSSQDQENDEIDEISQKKNRQMSSQNMESDQDGRSSQNKSGQQRQFMSKKTIRRMNSKIKARQSVILPRALNTSITTKISHVVESLPFSILMALVTLYALFGDDIRILTVNKDGDDAFFVLTILCMVSFTLEIIFTCICKHEYFCNFYFWLDVISTATMFLDIGWITDEWYSGDVTNAASIKTIGSASKVARKAARVIRVIRLVRLVKLYKHARQQIQQQQQKALLRQLLRQAQISQEEKMKQQQKQQQIIQESQQQQSQIQNQQQIRQQSQQQMSQSQNNQQPVNEEDQVLQIQNPQSVIESGKIKSNQLNQSQMSGNSIPINQQIQKPNSSQDGSEGSNNQFSKSPSKSTPGKSGQQTNSQGESSINGEIDTKESNVGQQLSDLVMRRVITIILAVLISIPLISFDTYQENINSYDSGIFRISQFKDQQNVKEILIKQYAEFHKGQIYPIQAVNILVNGSWVNYNYQKYPDYFEYTSLSPDQYRFTDLQYYIQTSQNGTLLAYSAADLISYNQTNSILSIFQTIFVCIVLALSALLFNKDVEDLVIEPIETMMKQIELIAANPLEAVNIEEQEDLVIEELEKSQDHKKLKEKEIMRTMETYILQRLIMKVGALLAVGFGEAGSEIIAENIKKGGSVDPMIPGKKIMAIFGFCDIRNFTDATEVLQQDVMVFVNEIAEIVHSAVNSFSGSANKNIGDAFLLVWKYDQFDYHPDPTNHQKLILKDTKLIKQKGDMAVLAFLKIITSVSISKKLEKYKKHPGLNARMKEYSVKMGFGLHMGWGIEGAIGSSFKIDASYLSPNVNMASRLEAATKQFGSIILISGIFREYLTESCQKQLRLIDIVTVKGSIEPMKIYTIDLSIKSLLKGIKQPIDKYDISKLTQREAKKYRVVQRYQRNQLIKKVENNQIQIADLFQTDEELVLARAPFIQEFYDYWDQGFQHYINGQWEKALPIFTKTLSMIPEHKDGPSNTLLEVLHSNGNKAPNYWKGYRELTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K5D1</accession>
    <name>A0A8S1K5D1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0110468</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K5D1"/>
    <dbReference type="OMA" id="NIQNHEK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="57"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="93"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="255"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8047516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="517" mass="60809" checksum="34C4A4A10EC2447B" modified="2022-10-12" version="1">MINTNDNLEDEFDGLTIEKLSGIKRSILQSRDVHDWFKKRYNDKVKKKYLYFPEEIKQQLEVKKIFQNFDQNKSNNLDMSELYEMFQKNGFKITEEQLQKFFKIVDKDRDNALNWSEFKNSAFNEQAAQVFYEIMKELRENMEKEQKLDSSSPAGKYMPFTFNNMISYLSYLASRDELKKAIDDTSISQLEKFKKYMEMINLNQTISVQKRNQSTPEVDIIHEEVGESQNLDQEQEFVKRNEQRFNLYKNSFYNSQSASKSPKSQKQPNILQKEQQLKVPKKLREIDAIVRSHQVPETQSLFKLKEKTNNSIIKLKQDVSKYIHLAKTQYNINVDELQIQSTKNKPPLTLKIRNFEEDFKNALAAHQMSYSNSEPYFKFNGSSVQEHQLPILEEKSKTITNPILPGDHLPISVLQELRKFQRTQQNSFNSNTFKSLFKTQSDFTKTAISQKSRSQGRFMNIQNHEKFQFSVPQTAKPNQLRSQLSTAPQLRVGTNYGMKNHQKIEIQPLLGKSLIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K930</accession>
    <name>A0A8S1K930_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Alpha-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0170372</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8051063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K930"/>
    <dbReference type="OMA" id="FINIQHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04515">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056861">
        <property type="entry name" value="HMCN1-like_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052969">
        <property type="entry name" value="Thr-specific_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763">
        <property type="entry name" value="ALPHA-PROTEIN KINASE VWKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763:SF5">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_25, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25106">
        <property type="entry name" value="VWA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="9">
        <location>
            <begin position="254"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-type protein kinase" evidence="10">
        <location>
            <begin position="469"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="7">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8051063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="907" mass="106209" checksum="C36620262DDF4E9F" modified="2022-10-12" version="1">MFQIETETSKICIDSIFCVKENCQNNHSRFYLGICLEYLRMEEEWYKNQDEDFEKKKCDIQECKLQHLDQSKLKSSLQQSINGIFLHNLCCKNECDCKLIHKPWANNICINDIMNVCQKKKKNNCSLNHLTWNQLKQKANESSKINFTTPEQLCESQNCNCQQHYPWMTDQKICIPFLQGFCPYIKCLKNHVEWEGLSPKQKIEQNSLQPCSIIYQELKQKANSFSQKQSIQLQCEEAKFLKFKESIFQSNIIDVVFIMDLTGSMQTWKIQMENAIDEIIQQFQKTNKGHQIRFGFVGYRDECDKNEKITYINLTQKVDEFKKQISKLEAKGGGDTAEDVVAGFEKALELNFSLHEESLLCTFLIADAPCHGREYHDISSDNQIDSVEKNYLENVLKKYKKIKECNFLCCIKINETTNKMFQKMQQVFPLLTITSQKQPKEISDQVLFTLKQSLIQSKSIQSKIKNTIQIQAQFNKYKLNISPPNYQYNNRAKYWQNFYNVVDQSLRKGGTALDIEQHINESLQSDDQNCSTYIFKAFDTINNMYIAIKIPKFIIDMYREGKLDKKSIDKAEQLAKTRFYSSSYACQMAHYFNQKISKIQEIPSLFYVLPIIYTLTEPFFGMNTVYGETFINIQHQFKKYTTNSHHSEPKYYYYSVFSHFSFIESGHTLVITDLQGCDNILSDPSIQTKKGLISILDFDETNHNEKGIEIFLTTQHQECSYYCSLLQLSREQFKITNSQKLDQTKWQIAQKTNIYGICITCSLFTQYDFDAQKQQQEKGYIMNCQICKTESNKQMSLDQHCNCCFDIYSASLNISLRNQTETGRCDDCKQLCSDLNQQCCYYCESYCLKNQKEMRVNEQLIRICSKGQAYLCQQKCAKCQKLYNNDVILSKDQYNKGIYKCIKCAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAQ6</accession>
    <name>A0A8S1KAQ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAQ6"/>
    <dbReference type="OMA" id="YDPNTYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="139" mass="15883" checksum="40090CFC4E7DFA6E" modified="2022-10-12" version="1">MLYDPTTYKGIKGGTQFNETEFFQVIANKAPKSKSIQSNLRVKNQSGILYKNQKQSTQSAEKKYTSPKIKSPLKLIAYKQSGSSCSLNSDEYISKIKQWNQNMMNKIARTQQSSPPKKGILEQCIDSYMKKQKFKQIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDR5</accession>
    <name>A0A8S1KDR5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDR5"/>
    <dbReference type="OMA" id="CHAKLQS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007204">
        <property type="entry name" value="ARPC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12391">
        <property type="entry name" value="ARP2/3 COMPLEX 21 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04062">
        <property type="entry name" value="P21-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="173" mass="19796" checksum="161C2617B192DD8E" modified="2022-10-12" version="1">MQQTVVTIDPSVCHAKLKSDKTLCGLAYFDDQKSQRGIVLESLEYFRLMTFFKNYNYEGPSDILLIYLWVVSAHMIKIIENDQDQQVAAKRLDEIAREAIPTRNGDRKNFLGGLLKETPGETDKIQAYLKGLKDILAKEIIAKFFKDGKRTFDAKFWSGIAKRKFMGLSYTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KE26</accession>
    <name>A0A8S1KE26_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2-hydroxyacyl-CoA lyase</fullName>
            <ecNumber evidence="9">4.1.2.63</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0200283</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2-hydroxy-3-methyl fatty acyl-CoA = a 2-methyl-branched fatty aldehyde + formyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:25375"/>
            <dbReference type="ChEBI" id="CHEBI:49188"/>
            <dbReference type="ChEBI" id="CHEBI:57376"/>
            <dbReference type="ChEBI" id="CHEBI:58783"/>
            <dbReference type="EC" id="4.1.2.63"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:25376"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an (R)-2-hydroxy-long-chain-fatty acyl-CoA = a long-chain fatty aldehyde + formyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:67444"/>
            <dbReference type="ChEBI" id="CHEBI:17176"/>
            <dbReference type="ChEBI" id="CHEBI:57376"/>
            <dbReference type="ChEBI" id="CHEBI:170012"/>
            <dbReference type="EC" id="4.1.2.63"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:67445"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.63" evidence="9"/>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KE26"/>
    <dbReference type="OMA" id="QETDMIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106359">
        <property type="term" value="F:2-hydroxyacyl-CoA lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02004">
        <property type="entry name" value="TPP_BZL_OCoD_HPCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000006">
        <property type="entry name" value="2-hydroxyacyl-CoA lyase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045025">
        <property type="entry name" value="HACL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710">
        <property type="entry name" value="2-HYDROXYACYL-COA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710:SF2">
        <property type="entry name" value="2-HYDROXYACYL-COA LYASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="13">
        <location>
            <begin position="4"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="11">
        <location>
            <begin position="195"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="12">
        <location>
            <begin position="384"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8053348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="552" mass="61326" checksum="778B81BDB5D73783" modified="2022-10-12" version="1">MISGNQVIAQSFAQNHLKYVFGIVGVPVIELGYAFQANGMEYYGFRNEQGASYACGAIGYLTRLPAICLVVSGPGLVHALAGAANAQVNGWPMVIVAGSSENGQQSYGAFQECDQWNIVKPYIKFGAKITNLRQAPILIEKAIRQSCVGRPGVTYIEVSGDLLRSNIPENELIKVPSFTLEQIPRCLADPKNILQAVQQLKNAKRPLIIVGKGASISFAEDQVIKFIESTKLPFLPTPMGKGVVPDSNNLNVSAARSTALGEADVILLVGARLNWILHFGLPPRFDDNCQFIQIDNYPEEFNNNRKTTTLFGDVSLVVDQLQKSFGNWQFNNKQWIDKLFEKRNKNTLINQQLMNDKELPLEYYSAFGIIKQYLPKDCVYVGEGANTMDVGRTIIEHDIPRRKLDSGTFGTMGIGLPFAIASKLVFRDKQVFAILGDSAFGFSGFEFETSTRYNLPLVIIIINNNGIFMGVDELSQKNNEKPVTALNPNSRYEKLCESFGGKGFLVETHDQLHNAMKDIINNPQQSYIVNVRINPYGQKKPQEHAWLTQSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJI0</accession>
    <name>A0A8S1KJI0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0220252</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJI0"/>
    <dbReference type="OMA" id="ISEPCEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000059">
        <property type="entry name" value="N-ethylmaleimide reductase, FMN-linked"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="4">
        <location>
            <begin position="157"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="4">
        <location>
            <begin position="516"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8054877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="881" mass="99471" checksum="E6D35FAC64487B3C" modified="2022-10-12" version="1">MLIGKQSQRKLKILFQYQERVQQRIIYNTNNLGQHTQNQKSKNYKCNDSLSVTQRDLQQMMLKIYPKKKNKLIPLESQILFQLSQYFFIIINFKYYLEKFFQFSKNSTQNLETKVIKQLSKMEKTNTPYQALFQPFKLGLIERTGCFSTTVVSPGLECKNRITLSAMTRGRCGQDLVPTDQHVEYYAQRAASGFLVTESASISDRSLAYGGAPGIFNDAQTQGWKKVVDKVHSVKGNIFIQLLHCGRQTHSSLQNSQAPLAPSAIRIRGQNQIVKQDYEVPQEMTLQDINLVVKQYKEAAERAKKAGFDGVELNAGHGHLPDQFLRDSANKRTDAYGGSAQNRCKFLLEVVQSLIEVFGAGRVGVKLTPVTHHADMNDSNPLELYQTLLRALSNLKIAYIVLKNENDPENFQDFGYPASKKQIPCVFSAFRNYFTGAIVANGGITLEEADEGIRIGKFDFVAFGQLFISNPDLVDRARNGYQLNKNIDWGSVFFGDSKGYTDYPKYQIPSNVLSELLNPLKLNDLFLPNRITMSAMDRIRTPAPYIPTELNAKYYAQRSTAGLIVCEATPVSQRSMSYPGSPCIYNDEQAKGWALVTKAVHDKGGRLFLQLFHGGRKTHSSLQNGLEPWAPSAIAIRGNCYSAQNKPFEKPHEMTEAEIQLTLQEFVDAAKRAKTAGFDGVEINAGNGHLPDQFLRDSANKRTDQWGGSVENRCKFVIQLVQKLNAIYPGRVSIKLTPIGHTGDMNDSNPIQLYTYLLQQLSKLNLAYVTLVEDKSQENAEGFGYPASDKQIQNLYKSLRPHYKGILIANHSITPERANQDIKDGLYDAVSFGQLYINNPDLVYRVRNAFQLNTNFDFKTYFGGDEKGYTDFPTFDEEKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKU8</accession>
    <name>A0A8S1KKU8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240142</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0240144</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKU8"/>
    <dbReference type="OMA" id="IYEYRNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8056104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="23664" checksum="7AA3360119A94239" modified="2022-10-12" version="1">MNFLFSNDDDDGNFDSFITQNLKPEPPKEKQSSKKLQTAFKPIEVKSQFQFISTPKLKPNKDQAKTSLWNIMLQHLNYTNQINNFNQERSKLPYPLNSFFLSKIQEYRNQKIEAIICEIHDVEEFGDTTEITLKDQSGSCKASILSDLFTDDLDDLNPTIYSPVKKIQKKEDILVYLENVTVFSPNEFQITIIINRANLKLAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQA1</accession>
    <name>A0A8S1KQA1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQA1"/>
    <dbReference type="OMA" id="LYQQTYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="131" mass="15910" checksum="EB98A16AA034BE48" modified="2022-10-12" version="1">MDNEIQFQNKVKRIKERYVHEAMYLVEQWRSLYQQTYIENGRQLKLTLDKAAEIVGVPRKTLEDYYYQLKKAETLVDLQNFKNCKIGVIRRIVKESKKQQETTTHLIDTNDFFLQENETNDYRKNSFEYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRQ4</accession>
    <name>A0A8S1KRQ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRQ4"/>
    <dbReference type="OMA" id="LISCSIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="111"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="41065" checksum="03AA62D940A54D33" modified="2022-10-12" version="1">MQQNEPQQPQKSIKYSIISQNLIPQRYRWEICMAINQQNSIFLFSEESTIRVFQFNGGMGLKQIQLLKNNRTSVLSFFPKQKDQFVTGSEDSSILIWSKQLISKGKFIQKLSGHINGVSCIIFNVQNTLIVSGGEDSQIKFWTPFKSNKEEQIRNISDQQWYCQQTIKDFEKAIATLSINEQGDKLISCSINQNILVIQKSSATQMWIVIQNIKIEIWGRQLQFINDKNFVFVPYDGKQLYLYRLNSVNNYTKKANFLVSLGQQYYDINPFYFKDRNILILQGNLFLSFFRIKGNKKQSFKKKENFEIEKELQIDFQPGFLKAILSLDGKYLITFHQKQSEIQVRQFQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUV2</accession>
    <name>A0A8S1KUV2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0280271</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUV2"/>
    <dbReference type="OMA" id="SKFMINN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8059179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="60" mass="7012" checksum="DDC69A34FF9DD486" modified="2022-10-12" version="1">MGCSIQKPKQNQRNHLDQIERLIQSDCLSNESKFMINNNPIIKRRNCSKEKKNLQGSLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KV65</accession>
    <name>A0A8S1KV65_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0280326</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KV65"/>
    <dbReference type="OMA" id="SKHMYGC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="4">
        <location>
            <begin position="79"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="138"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="174"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="211"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="247"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="285"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="356"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8059289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="434" mass="49883" checksum="A7EE49A6517C006D" modified="2022-10-12" version="1">MFQDPEEKDMIGDLLNDSFEADKTKKSYSNNRPPALEIKQQQKASPVYQPKVYSPQVQHKANSYDLDLRLTKIPVQQSAPNSALKAKSYDCMTQFIPEEETSLDGDLISKCKDQNGARSIQKQFQEGPVAIKDLIFSRLEKGFVSLSKDVFGNYVIQNLLENGTPLQQQKMLVILQPHTQQLAFHQYGCRVLQKLLQNAYKTPEFQVLFDSIKGKVKELVIDQHGNHVVQKLIQLMESDVSLWVLDGIEGQISKLVTNSFGCRIIQKAVSISNNHVERQMRVLQEIMKISQELCTSQYGNYIIQQLLKDGPEVIQIEIQQIIMDKIEEYSLNKFGSNVVDCAIKCSDNKFKLKIMELLLSQKNHPVLFVRLSKNAYGNYVVQNFLKFADSEIQKELYLKLTNNQQLLQEIQQSQFGQYVYQMLTQKLELEAFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWP1</accession>
    <name>A0A8S1KWP1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0290106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWP1"/>
    <dbReference type="OMA" id="YICKQIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034078">
        <property type="entry name" value="NFX1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR, X-BOX BINDING 1 NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360:SF12">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR NF-X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8059668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="439" mass="51542" checksum="1078ED820B38BE2B" modified="2022-10-12" version="1">MKNQLNICQQEIVINSSNSKNKIKLLCEHEVDENDKIDVNTYKCMIKLKLKFQQCEHEVEYYCFEKDKIDNKCHFICDKLLPCGDRCKKKCYQNCLPCMNNCQGLKNLNLCEHFVESRNIDQEQQKQCNQEVKVKLDCGHTANFQCWQREKLQKSYICKQICNKQRICGHSFNQCNNYCFQQQCSPCLQQITLKLDCDHEQQIWCYQKDDYINTVCKECQNKNINNQKKNLKNQVIIDLNCGHKVQTTYDQKAAVLQSFQCLEKCIKPRICQHNQPCDKFCYQECTPCREIIEKELSCGHQAKLQCSNAKQELENYKCKQPCNKKWPCSHKYSCQKECGDHNCTPCQQEVTKQLKCAHTITEICSNLQKYKCRENCTQTNQCGHPCRKYCGEECDPCEQEISIVFASCAHEIKIPCFQKESLTKKKCQQCIEKNSCILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXW5</accession>
    <name>A0A8S1KXW5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0260068</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXW5"/>
    <dbReference type="OMA" id="GWISKRS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF3">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8057486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="427" mass="48200" checksum="00A0D858A138F488" modified="2022-10-12" version="1">MYSEGREDSQYSGNNMEQYDEDQDQGDGQDVDMEGQQYFDDEEQDMQQQEEYQEYQPEEQQDDQQSFKYQPFDQRKWQWAEPLIEGVPPCARGGHSATLSGASIILFGGHYYANKDEGYKYLNDTYQMDVNANRWFKAKVQGTPPAPRYAHSAVLAGQRIIIFGGKGEKCVFRDLHALDPLTLTWYQGPEGSGSPSARFAHSATLYASTKMIIFGGWNGIDYFNDLYALDLEVMAWSQPPCTGPAPTPRQGHTAIQVGANLIIQGGFYYQEDKSLKTLNKTANPRHGSYLRSCYLNDIRILDTEHFAWSRLRVSGTPPAPRYGHSANVSGADIVVFGGWSLNSGARSENNFATPPDIDYLIVLNTEKMCWEKAKYEGNAPRNRYGHSATSIGPHILIFGGWEYNRATNQVVVLRDLNVGQQQQEQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYX4</accession>
    <name>A0A8S1KYX4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T0270324</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYX4"/>
    <dbReference type="OMA" id="NMLIHEE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="123"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="407"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="409"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8058642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="464" mass="54479" checksum="94B4160D29DC9CD2" modified="2022-10-12" version="1">MDYTIKDSFFDDSEEKSFWTQTQFPSKDIQTELRDNMLIHKERELVKRKVGIMSHYLLLFKDQEVHKWVNLINVTMEFIKNSVAGQGIRFLKNKQHFEMYGDVEPWYNYLKKYCIQRGFSQRYSLLKKIGQGNFAEVFKAICKTDGQEYAIKCFRKQDLKEEVDKLSIIKETSIMRKLQHESVIKMYEVFEGEEYLYLVLEYLKGGELHKYMKKSPPFSEEKCSKLIYKLLKACQFIHEQGILHRDIKPENIMLRKKDDLEDICLCDFGLADYYTPSGKYLFTRCGTPGYVAPELLQDKIYDYKVDIYSIGILMFILIAGKSPFEGKDYDDVVMRNYYAKVKFEDCKLSEHGMTLLHGLMNKNPVKRLSAQEALNHQWFIQDKLAKTCQFKIRRHSNVKRLPHLDGSPIPTNNFLSPKNSFNNLSPQSLTRTDDNTPNSPITPVTPLQNKGKPIRKSIFNLQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L098</accession>
    <name>A0A8S1L098_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0300041</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L098"/>
    <dbReference type="OMA" id="MWGETNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="388"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="658"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8060251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="784" mass="90463" checksum="A997D324BBF539A4" modified="2022-10-12" version="1">MLYIFGSLLISHLVTCIVLTFYQLNDSRLVNTILLSFQFLMLAIHFIQKIKQIQNEKIQISVKVCIKISLLLLIFQRKQNLVQSFLAGSYMQYLLRKQSIFLKLIDALVCMFYFIMWGETNENTVTGTLLSLFMVLNICMKYYIQNKKEKESRGQISTAKLPSPELIKNQTSPNYSRKMSGHKYQSEPSQDLQINDFGCMDQYQKIFNMFPDGIIMVNMKKQVIFINKHLKKMMGGMNKKAAFYNLLQMKNVQQQINELEKINSLFQEQQIILNEQEFDNKTYFKKSTFDESRFDDQSELKSSYSFQQFENVKKLLDHLIENRHEKNGVLKMLCQFPQNEKSLFQVSFKRFENKQSEFILLIVRDMTSMNLVTFLQRVITDKSNFLSTVVHEFRTPLQSIKLMVQQIQPMCSEEMVQKYLLPMLSQLDCFTNLIQDLLDVSVLNVGKFKLDIKQLNLHHLITETYNIMVVQAYAKNNDLIINLKGIPEKICSDPYKIRQILINLLGNAVKFTYNGTITISGKAINKSVEITVSDTGLGITAANQNKLFTAFGKLDDPTNSNPQGIGLGLMISNVLARKLSYDQKGLSAVSEGEGKGSKFSFQVYDHSFSEEKQKYQQESVVRQQREYFQAPLNQIKQCDSFRMSGLELSQYSRCQCPEILIVDDQPFILQMLKIQLESQGYRVNLAFSGFECIKKIEQFQHGPSCSCKNYKIVILDIDLPNLNGIETCKRLISMGFNGYILGHSGYSGGQEEEECLKAGMVGYFVKPLDIAKLQQWLESNRIML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2E7</accession>
    <name>A0A8S1L2E7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2E7"/>
    <dbReference type="OMA" id="QEQEYKD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019">
        <property type="entry name" value="KDA ANTIGEN P200, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="551" mass="68280" checksum="FD7C677A2D3898E6" modified="2022-10-12" version="1">MSSDYEQDFYNITQATLTPRLQEACRREGIHPNELFKKTREQVMQLIKQRDTVGAQLTEDMVTEIEKHLEDKRKRRINIVRSQRQKILQEDNNVSRNSNHISNLNLSTMSFADKQQKMLENIKRRQQMEIEKMVEQEQLKERKSQIALQKMLKQQEKEKQFHDELQKKRYQNEQLRQKQEMERKKKEQREKELLEQRTKENDLKEQKRRQKEQESMMKQKQEAEQRELERRKKQEQMQRENEQFQNEQQKQLEYKKQLLEQKERKRIQMMESRTIERKTQAEKERAELEKKLQMVKQYNENLLEKKRNDYSVKEQHVEQKRREFEEYLEKVRQEKARQAEEKQKQINKIMQTNQQKEEEKRQQYEHKQQEYQKKKEVMSEQQRQQMEEKMKKYRDKEQKIQMILQKNEMMQEQQKQELQKKLDRTQLKLQALEQKKEEDQKQKITLQIIKDIDKFDNLTRMEQLSELRRTKLEMKLSKDDERTEMVRRERENLAKQAEIIRKEAEEKRQQFIQEFEKKKKLQGITQEGQRDKQVSVNNRSQSSGINMRQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L423</accession>
    <name>A0A8S1L423_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0290009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L423"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8059476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="86" mass="10137" checksum="A3D6C1ACDCD34296" modified="2022-10-12" version="1">MKMRCTQTDHRNQQIIGFCINNTCQNQRPYCNFCLPCHGEHLNRLTSQELLSEWIKERILIIQSIQKAVEECKVTLDSLLKFITLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L558</accession>
    <name>A0A8S1L558_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0290216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L558"/>
    <dbReference type="OMA" id="IDSWIHM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="15"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="58"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="100" mass="11423" checksum="1B44423F630D53FF" modified="2022-10-12" version="1">MNSNEPKPQTHQAKARLKAARSIFELADTNKDGYITFDEVPKLLIETNKLISDEKYVPTKEEIDSWIHMTDLNKDKKVSIHEFEVLILKALQAQGIDLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L7L1</accession>
    <name>A0A8S1L7L1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0320032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L7L1"/>
    <dbReference type="OMA" id="LESQDTF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000051">
        <property type="entry name" value="Protein phosphatase 2C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="172"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8061592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="458" mass="52774" checksum="755A45B366295804" modified="2022-10-12" version="1">MNLRIFDKTKSASRRLKSPRIFKTEGNYQQQPRENKNDNQNKPKSFINGKSGLGKRDYSENQSQITQKRLIVNSYSQQTRQQQQQSNHTHTKYNTFLDSILKLSKSKEMNRVPIRNRKQMWHNYVNPESSIPERRVQSQINILKTDETYNKNRSTASLCSLESQDTFQFNLKYIHKTRQGQIANNPSKVNQDIFYCQTNFIENFHLFFVCDGHGQNGHLVSNFIQTNLPNNIQKDKLQLLSHQIKETLQKSIQNTSINVNKQSFDTNFSGSTLNGILLQENGKIHSFNVGDSRTVIGKYNGYGSKFKPYQLSIDHKLTIKKEQYRVISSGGKVDTFYDQNGNSIGPMRVWVNGTQYPGLAMSRSLGDQVAQSIGVSSVPDIIEYQLGLNDKFIIIASDGVWEFLDNQIVVDIIGKYYQQNNIEGASEELMRVAYRMWTIDDDSVIDDITFIIIFIQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LAD1</accession>
    <name>A0A8S1LAD1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0320119</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LAD1"/>
    <dbReference type="OMA" id="XRVVRKS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3450:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.310:FF:000008">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045059">
        <property type="entry name" value="Ribosomal_uL29_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8061756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="14635" checksum="013D33FB7BE9F3FD" modified="2022-10-12" version="1">MSSQKFKVKKLRDQKPEDLLKDLEKLKGELIQLRTVKVSAGNAQKLGRIGLVRKRIAKYLTVINEQRRNSVKNAAKSSTKLPVDLRGKKTRAIRQRLTRSERAQKTQRQWKRLNNFPLRKFALKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIU2</accession>
    <name>A0A8S1LIU2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0390232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIU2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="122" mass="14522" checksum="553FBCB4357D395D" modified="2022-10-12" version="1">MVKMDIKQLRDLTLSIQILNQNKIFHRDLKPNNILMNNGYPIIIDFDCSYFWEPKLEKWLRGKGLTTKYYPENDIEQDKIDIYSLGIIGREFVENCPEQFSIGATLEYQDRYSLIQLEKLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LNL6</accession>
    <name>A0A8S1LNL6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0400037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8066953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LNL6"/>
    <dbReference type="OMA" id="HQVKVME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="198"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="300" mass="34994" checksum="DB8FA34B53838C87" modified="2022-10-12" version="1">MSLNDWKNEKAMLEQRNQFLYMQIQEIKEREEHQKKFNDTIMQAWSQGDGRTNCKVYKEIQSTLEEYSRELIEAKKKSSIAMMKLENENTELKYQIQQLESIYQQKNQLHQVKVMELKKRIECQNNKENVGDNQFGCIVSVKSRTCSQNTSREINKQRSKTQNKPELLNCRNSNVMEINTDFLKDYSMQIESVSPQERQNLAIQLKQLTKELKTTKNEIENTNLQTANELKTEIKSMLNKLLKAKGKMTESIVGSGKESVLMLRGSQYKPTLADFAKQLNAVRSIETFNILNPGKVTNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LNQ0</accession>
    <name>A0A8S1LNQ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0400058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8066993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LNQ0"/>
    <dbReference type="OMA" id="MEFENFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8066993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="351" mass="42212" checksum="A4407F41F3EA6D0A" modified="2022-10-12" version="1">MINEQTIQDMVPYIWPEDYDDRLQFSLDLKMQFENFIISNPKLKNQVLQTMQKISEEAPYLDLFDIFKIEKERTHKLYKVLYSLHSNNNTQIDFKQPVPNKTLQKLLSDSYRLFNRSILDYQCKLISYAKYQKIAVSLLKKSINQMYLDYNTINFVCSCATKQCITLNRLWKDYDDTGEKYKKLKVSLQKLEQMQKPDDLTHYFNRQQLILIYTLFSLMQCEQTIAIQLGEQYLPFQYIPPQTHFEEQTQDFTWNINGFSKIRDYFPKLAHKLNKKMKLLKDLPLFTIQERIKIYQYTQHQLGYKQIDDIHINLQQKIIDILNYRQINCEIELILLVANAKCYTQILYSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPT7</accession>
    <name>A0A8S1LPT7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0390143</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPT7"/>
    <dbReference type="OMA" id="CTTRANC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.30:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerases I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.30">
        <property type="entry name" value="RNA polymerase ii, chain L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00615">
        <property type="entry name" value="RNApol_arch_Rpo12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006591">
        <property type="entry name" value="RNAP_P/RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039747">
        <property type="entry name" value="RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023464">
        <property type="entry name" value="Rpo12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12056">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12056:SF2">
        <property type="entry name" value="GEO11084P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03604">
        <property type="entry name" value="Zn_ribbon_RPAB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00659">
        <property type="entry name" value="RPOLCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8066546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="54" mass="6219" checksum="1CB34949837E3FF3" modified="2022-10-12" version="1">MDIQDASRVVYICGKCGKDVQLEAKDIVRCQCGYRILYKKRKADPKNPPQYEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LS38</accession>
    <name>A0A8S1LS38_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420089</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LS38"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="70" mass="8894" checksum="9EC64202F7AF7303" modified="2022-10-12" version="1">MHQHYLPRPQDWKSLIFRQFLYITKNMEQKIFQDILIPYNFDVQEIQQQFGIYQKQIKQEREEYEQELKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSB6</accession>
    <name>A0A8S1LSB6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0430190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSB6"/>
    <dbReference type="OMA" id="LMYQVTL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000810">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF68">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATG1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="290" mass="33566" checksum="04111ACD9C07DCE0" modified="2022-10-12" version="1">MQQVQALPPQFFQALKIQTTLGEGHDAKVLLALETKENENKQYALKCQAQKIQDTAINLFNEIQILIHIKHHHIIELYGYSQDYTCLVLQYCPHGTLVQLIKSSPLPVSIAASLMYQVTLALTYMHEQGFTHGDLKLSNVLIDSQYNIKLCDFGFARWNGKMAIEKQTIAGSEGYTAPEIWTSNPNYIKSDMFSVAVIFFILITGHPPFESNNPDNNDPWWNLIKNNQWDTYWKDIRSNIDQECKNLFVQMFSIQPEERLSANHLNQWFKLKQTTQDNLIQEIKKRLAEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTJ4</accession>
    <name>A0A8S1LTJ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0410337</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8068126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTJ4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="131" mass="14964" checksum="BACFDBBAD6EF79FF" modified="2022-10-12" version="1">MQTQYPPKGGVNKGQIIPLKSSCPFDCQCKSEEIRNWHHKSCSELCFVSQYGDIFCQHHLKICDGYLIQDASFECSSAGKGNTWKQYRGNLQFFMALVLCIKAAEISLDSNNELINFTTSLQKEAFQRRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M1N6</accession>
    <name>A0A8S1M1N6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0520196</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M1N6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="69" mass="8124" checksum="03DA2B5A3A6B1DB9" modified="2022-10-12" version="1">MNNNQTIDLLKLILVQCNNQIIKLQESVLKKGETIVYIILLKIKTLKKQIRMIQNLFQKGFEYSKEKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M2M1</accession>
    <name>A0A8S1M2M1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0490192</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M2M1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="367"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="154"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8072565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="51390" checksum="7A1E2C78A2B97ED3" modified="2022-10-12" version="1">MNKKSLTQTERATSAAIGKTQISTQHKHTFMTQTQKTENLLNQIVEQECPLYTQYKQKNPNYPIMKIKEFKKPQIDPVDHTDYIKFKKMKNNFVKEYKEKLDNYFQNNTKYERRFFKIYHRKDQIYFKHKNSSQKNVENPEVTDMRTYNQILQMNQQQTQRANLNKQQEKRRRELKMEEKEEKEYIKLIMDKFLKEEQKYFDKLTNLEEQGQQMFQEKVQLRNKYTHDYIKNPQREFVEKKDLIISKVLQIPENLNSSVISNAGSQIQSQPTIYQANMPFSSAFSSFQQLPSLQNVLQRMNSNVLYQLPVPQIQEYDLQPGEQIKKPSTYQTQQILDLQRLITLNEKIEFTFRIVSEPSLLDIVDSNGCTLLHQAALKERNGIAKFLLIKGIDYKKTDNKGRTALDIAIKQKNQKLIDYINLIKKKGLKEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M412</accession>
    <name>A0A8S1M412_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0490201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M412"/>
    <dbReference type="OMA" id="YRIYTNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035931642" evidence="2">
        <location>
            <begin position="20"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8072583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="526" mass="60619" checksum="2BE4F804230F2568" modified="2022-10-12" version="1">MKWTLIIVAISMYALHANSDLDTVKQLLTDLKTNVQLDIEALDAAWELHKNNKQSVIDALGFSTQEQRSECSKKDEDVESKKRDIKITNDFIAWMEKRQRLNSNRLGVLEQNRCQSSNNYVHDIKNYKIALALVKFLREQLDKLDNNASPVQKQEFLQKVTKFVQIYQTGKLLTMVENLQINNDGSYVLPTVEGDEYDLNESILSSTRGKRNTNKILIQQQQTTVIAQNDGDDKTDSAVVLIPSQCDDSINTVIVVQNDDGTGNDDDLHIKFTNGGSNTNSHTKPIPKPHVDPIHKHHSEEYVSGEEFIDNDQEEVDDNEETDDDNNTEEQTHNDNDDNNTEEETNDNTEEEIHDDDDDDDDNNNEESNHQDNNDNTDNDQKKTGDDEQGSKPSVTEEEDLSDIRKILDAIEKHSRKSLDLEQEDEVRSSMIYIDFKLHIELENQYFNKQIAGEKENLIKLTNQLTSRVSISRQCNARLKQISIAFQVSQDDYNDSYQHYLELRKSKEYELSTFEDIYRIYTNQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5H2</accession>
    <name>A0A8S1M5H2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0450255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5H2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="35482" checksum="7D52F96CA0DE1867" modified="2022-10-12" version="1">MGNYCSEICNEPEIQVLRLSDLECTPQSVRSRKSQQHQKGYFTESKEPVLHLNNNTEKQRRPPYRFTTGGVYEGEWVGNKREGIGIMTWPDGAKYEGEWKDNRANGNGKFWHLDGDTCRFILFLLDEGEWKDDQTHGYGIYVHVNGAKYEGYWKNDVQDGYGIEIWADGSKYEGFYFMGKKDGQGKYYWPDGSFYSGAWKENNINGHGTYQWQDGRQYVGDWLDNCMHGKGCYKWKDGRSYLGEFVQDKRHGFGVYEWEDGRKYEGFWDNGKQHGEGNYYESNGKMRRGLWEDGKRIKWLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5T0</accession>
    <name>A0A8S1M5T0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0500179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5T0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106245">
        <property type="term" value="F:L-serine-phosphatidylethanolamine phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006659">
        <property type="term" value="P:phosphatidylserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004277">
        <property type="entry name" value="PSS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03034">
        <property type="entry name" value="PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="129" mass="15466" checksum="B558122D02DA87F1" modified="2022-10-12" version="1">MRFQEQILFFISACTLIYFAYENDYDNMKNRYRGIIGCILFICMMGSLRPYNGPIKRFIGYWRFMFWLGVCYNAFLIFLIFQNEIEARQLMKLGDQSLGRSVTLEHQFQENFFLLQVDQESSKISLEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M7T6</accession>
    <name>A0A8S1M7T6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0550106</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M7T6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="119"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="813"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="816"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8075849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="876" mass="101111" checksum="7D1BF7774DCA86B5" modified="2022-10-12" version="1">MNQSTGQNQQSQPLINYGNVKYSFKCLRRHLLKDKVYHVLALQNMITIGEHLNDPNPKYKLELNLKNTVYWKQVHGQNKAFGIKYKKNVKYFEASTEQLTKFREAISCFIGFHSIDDHYKIEKMIGSGSFSQVHKVFRLHDDKAFAMKQVSNQQKTDKENMQLLENEIGILHSLNNEAIVKIYEVFRIEENQYGLIIEFVDGICLATLIEQLKKNQNQLKEEDIKILLKEILKALNFIHQEQVIHRDIKPQNIMISQSSFKNVKIIDFGLSIKNSQQYNRCGTPGYMAPEIVNMKKEDQKPWTSLCDIFSLGVVFYKILSKGINCFQGQTSDQIVANNKKCQIDWSNVQQFNYSDNCISLLKAMLAKDPEERISADQALQHPFFIDLPPVLETDFAGNDMTQQSKVIVNQIIDKPNLDSIAESLNDFSGNQKQHIYVREFKPSRISKENSKEFSDFSILKTKNSETQVVQPIDNPPPIKFTQQEAEEIIQKFRVYKPQNKQFKYENLLKFQNCKLKIYKDSYYFGQLVNGKKNGFGVILSNNGRTYEGQFENDRKHGSGFERFPDGASYNGTYINGKPDGVGKFLWANGEVYEGGWQNGLKHGQGIWNGIKGDSYVGEWKMGNPEGYGVHVWVNGDRYEGEFHNSLKHGEGIEYLINGDVYKGQYVNGKPEGVGEYQWSSGSYYNGTFSNGLRHGKGLWIKDRNATLTDNYDGEFVNDKKCGYGVYKWAKGSKYEGNFYDDLRHGYGSMFWNDGSYYIGMWEQGYQWGEGEYCKKGEQPKFGTFEKNLLVIEDQEKLSQYHSKLPPQIRIRQYTTSQRSVRSNSQRPSSQSQQRNVRPSSVKSVNQIKQGSMGNNQFLCKKRRQPSFTKQTFDLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCJ7</accession>
    <name>A0A8S1MCJ7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0550137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCJ7"/>
    <dbReference type="OMA" id="LNHICIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000267">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF644">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="1">
        <location>
            <begin position="445"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="764" mass="90548" checksum="84B29F4D2BF671FB" modified="2022-10-12" version="1">MTTILDNVTTLKDKIKILDDNKNQTKNLDLQAIYKHIHNIGQDSNQLLEHALKINFGNVLKIQAQNDQNEVLQVFKKVCQTAKDLNHICIYVKYIQQISKQDLQKIYFKELGFQTEKLITEFRKQGTIIKELRLDTNDITKQRFMIQMITTLCPQDLGFNPELFSLMLIPFKCLHKADPTDISLLEKLADVLLSQCNGQGIANQNQVHDWLALLTGIIQYAYEGNSIGLFILLRKIPHQHINELLYRHKYFMLLNYFNNLKVTLKIFQGLTFYSITQQSIFFIRTISQFLYEYALEIKSKQYSDILKYIYQEFILTTILGNIQMIHYSTELIDLLLFYIVGMSSIDQDPLLKSFNLLYFKLKQASNYKIFTKDFKTQRIQFLLTVAMNYNGLNTDEQFLNIIQIYQTTDKMIPYKQYIQWDFSQLIEQQSQIDQNILLRDSKDFVGLENLTNTCFMNSILQSLYMTQSFRKFVIQLKFTDQSLQLIPYPSKLSSLQKLFLLLTYQKQGYCSPYELKRQLREPYSNTNDQQDVSEFAHHFLEDLLELLPKHLQIPMEKIFFGYHRSCIECYNCPKRQPAYRPKEKFLGIDLHFNEDEENQDLYDMIRKAYEKEQIEFTCDKCNQRTDRVYKSQQLIQLPSVLFMIVHRFTFNAATQTMNKMLTKVPFKFQIDFRDVFNQKKLNSKDCIYDLYAFIVHMGKNSYSGHYICYARQLNKPDVWVTFDDTMISNLEYDSDQLDKELIAEETPYLLFYQNQSGQPLIFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEA0</accession>
    <name>A0A8S1MEA0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0570008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="TIYGMIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035875005" evidence="1">
        <location>
            <begin position="21"/>
            <end position="1532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1532" mass="176622" checksum="CE07F74FFDE9AB8A" modified="2022-10-12" version="1">MLFLLLLPLLSYSLNTKVKCIIDPLTNACDHLTIYFNPTYPIRINSLIQLTLPFIIQGQEQLLLTSFDESTVALLTLTGSTHFTVTQILPENQWYKCNLTLTTVLSKKQKGIGQFQMNIVVSQYSNTLILEKGPQIPLTYSVINQIYTPPSIIPIPTVIPSPSGTLCVTSDWGFVLNKNIIRQQQSMTFTLSYDATNDRLDVILTMVDLNSGFIFHRECYFSGPQVEKFTTECTLKQTGSKLIFQLRSQNSVLLTQQTEYLLEFFISHQGDTIAASTFTVEIFHDTQLIDQCSSSKTFLIDETDLPVSLNQITEVELYLDTPSIGVPTFMGVAFRPKNIQIDTLIKIELGFLSYDQLNCQVFQLEIDTFMPSLSFSHYSYDSQNLYFYSNKNKPDQIQYKYSVNCTGITILQTTNKIFRVVYEDLYHTIIQYFQTRQPQRLPTDSQSQSASLKIVKNIELISKKFNNPLVSSIITIKIIPIYGIYRVLIEFPSSYTTNIIKCVINETSSVFCNQDSLNPNRLIVYLTKYHDSELIKDPFNVTIYGMINPYQLKYNSRICVMLDDDNILTPLLFGNENYLNGITQYEYLDDYINTNIYKQLPTSDFKFSQSLARVYDLYLIGNITFSMGSINEINKLYLRISDQYTIYDLNCQLIKVGDQQLNNYISSCNQNQNYIEMNLITDTGNIRFSQQYYLNISNFRTQDLISIEQYAALQPMIQLFLAQDDNIISLENQVNGSFVMKTQKIPFYWYNRNADGEFNRLEVQPGFEDTKTYKIYLNHFGLQRLYLGLADVSDRFNCTINLQLPTIHEDIVVFGQSSKYAKQSFSSQAITQPKYIVKSMTDDTFYETIKDTELVINPGDSGFIFNIASKSLINIKIRVQINYTISFPGRNSWNPIYYYNPFTEIPVLHLEIINKGCELIPQKSTYDLPVNGYTHPIIIDASECIPVVDTKLNVTIQDNNKLQLVPIQMSISRYRFELITRTTRKAPEYSYIFQIKATNETISGIITQVLINNIAISISAILQYTEYPKASPLKGLYSQSSNTISLSMTCSQPSEILLVIGIDEITDESFEKLRSNSISNELTGDYDLFKLQPENATLQQESSYLFKYEIMPTNLIMDIYNNRIGNQITYNDYDIVSQVNQSQIKDNSYKTLMNSIRRMMINRLSLLDKTSQLHLQRIQSKLNYFDTTVTFALSNLKYNTKYSFGYYCKNLNGNTSEISTNFQYIANETTSNNKTVSKMLQLKFKQKISSQQENNILCALAQFFSINYYQVLSRECKQCRFQNIHYDKINYTQYLQLVQDFVPFENRSTTEKQKYLDNLYTMKIYFNNYPSSNGQNFDITQKIDKSINLTNETILSIINVTDDFSLTFNYETFSYQELKPAKRSNVELATQLVLLNYEFKTISLDISIKANQDVLGIVGLHKIEPIFENVIKHPTGIMLRRGILSTFSNISEGVQFAELNKNENTTLTFKDLIDGQKYLIYYSSTDLQNDCLTKQYQETVVQQITFVSQQQSQESILHINIALILLVIFCMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MGV5</accession>
    <name>A0A8S1MGV5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0620242</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MGV5"/>
    <dbReference type="OMA" id="NETMFFL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="323"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="696"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8079870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="839" mass="98070" checksum="264F4B32E044F24A" modified="2022-10-12" version="1">MNSTSVKNQNETMFFLSIINLIFGIIKLEMFTIISNSILFGLMFCKQNQCLQEGYLGYKYKSHRKHLIFEQVIVIYYCCYQLIQRGVPAIWFNIFSIQLDQHLVLFVTLIIVIAFTCAQVQPMVEISTLQTILEQLILIIIFLYNKYNFTSLRQQTQNRIIKQIMKNVRYYAFDKYQNEIDGEKLLQSEQILNSNATLFSYRCENQKLQQQIRKDLKIKCYTMQQIDIAQESQIFYSKPSVFETLGGFLEFYQKDKHQMKNKVILASEYDELIGQKQSYRLYIEQFKQFQRIYTLVALVNLDDKIPSKQNKNIDKFKMSLSKIFTHKLKTPLNTTLGFLQALVQENNTIDEQVKNSFLKPAFINSKIQYYQVQDILDYVNSVKLVTYNVSKVNLYKTLQLIYDMIELQCRAKKIQVIFTINSKPFDVNDRPIYLNTDQLRLERILFNLLNKSYRHTQIMGKIQLNVDVLSENNQVQFKINDNVQGFKQEQFEQINNFARLQNKCIHGFRNSIVKRSTFKFSLTLQITNKLIYFLSDFQCSLQINHDLEEGAIYQFFISLNCQQVNIQHSFEISQKNSSFQKRSSGSLKQNLPNHISLFTKSQNQISDKVLEEYEIEVEPLANKPLQLSTQVVQNYPQNALFQSHPIQQSVFPFQKSVFQKEMVNDKSLTISVAFDHSFNVKRAADSIDIRQDMNQTIMLVDDEPFNHDTLKLMLKSLGFKNFISAYHGQQCIELVKKHYKSIKVIFMDLDMPIMGGVKATQILIQQMQDGEIDHIPIVACTAHDDKDTQQECFTAGMICIIAKPVFIRSLQETFLRINEIKVKSQNLVFGSKGITSSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MH02</accession>
    <name>A0A8S1MH02_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ABC transporter family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0620264</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MH02"/>
    <dbReference type="OMA" id="RENWWSA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000610">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000140">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="11"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="308"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8079910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="576" mass="65674" checksum="62B0E7AFA7E4D88E" modified="2022-10-12" version="1">MEVTHEILVMLYQRVISLFQYYQVLGQTNSTFILLSSLIIGLLVCCFVKYLLNVIQVQRTTQNIHQQMLNSILLAPIQYFDINPSGRIINRFSNDLSLCDNSTNQVCLDILELIGNFFIALITLAVLQPYFIFMICFIIILNLYQYHFYNKIVSQLKENELIQRSPLFDFIKKTIGGSIQIRVYKQQDLFKKQFLDLSNKCNLNSLTYCYSIRSFCFNIDFIGFIASSVGLFIFLNLNSNDVAIFSQGLLLLITYNDGLSLGLKQLINFATQMSSYNRMFQIIDVESEAPQVKDIDSKLPNFPESGHIKFNNVCMRYRENSNFILKGLSFEVQSGEKIGCVGRTGAGKSSILQAIFRMCEIEDCEDSKIEIAGVNTKQLGLQKLRSSIGIIPQFPFLFTGSLRSNLDPFGQYDDQAIWEALEVTGLIEYARSFSKGLLTDICDVNSLFFLGQKQLICLARILLQKKKIIVLDEATANLDMKTDDFIQNTLKQQLKDCTLFTIAHRLNTIADYDKVMVIENGNAIEFDTPFNLMAQSLNSTTIDRKTQFSYLVFNTGESNSRVIFDIAKNKLINSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MP36</accession>
    <name>A0A8S1MP36_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0670005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8081990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MP36"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF10">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8081990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="330" mass="39148" checksum="ECC467EC26BB1C04" modified="2022-10-12" version="1">MNTNKSQETSQSQVIFIMESQLQLTVCQIILDQFQINTTQNYTNTKEQFLIYLLTLQHIQTLVQKQQLLLEQLMILLVVVDYVEGVFWYTETILRKVLQEKIKPILMINEIGSLYWNYKMIVKKCIRNSLLQLIIYIQIMFQYALDQEKNLGLFDVLNLLLSIQKNSNVVPPKLQEKFWGDNYFIVVSKCWWKKSNDDSGKQLQSAFTAFIIDQSVNWLIQSQKEIANKMFYILGLELTQEEQKLSGKFLLKAVMSKWINMADTLIKMIICHLPSSEEAKKFRTSFFQITQKKLKFDFLLLVEFYRQHKYKIIDQNNGSKLQAREDRRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MU40</accession>
    <name>A0A8S1MU40_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0630185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MU40"/>
    <dbReference type="OMA" id="ISGCETC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="120" mass="13823" checksum="12F2008DD3DC7336" modified="2022-10-12" version="1">MSFPEYFQISMKISGCETCDSPFIEGGPDMIIELNYSLFIVKCDQIWELHGICGTYLEVHKPLNKDIIYEQQIKGKGTLKTQMLTKSLQSGRYEIWVVVRSKIGSVIQYVKSFYITIVNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MVU4</accession>
    <name>A0A8S1MVU4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0700112</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MVU4"/>
    <dbReference type="OMA" id="RYLAYQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="410"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8083542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="457" mass="53934" checksum="00EBC13C493E1FFB" modified="2022-10-12" version="1">MRKVILKTKIYSCILIILSGTVAVIACILAAQVDDQEVKIYGTLYTIAALIFMQINLYIFYQLHTINLINAEINNFNCLSSWKVLHKSILNNSDLWFSKYLLKCLIVIIFHSTLSFITRLKYSNVAFEFDQNMSISIVLFVISFIFGFVVQIHFVIQLITMLADFKEYAKEIVFSFHIKESLQEEKIYKRAITSMAIIFVLVSIAYYTLKLVLYIMIIKNSEETNTKVFSIIVSILNVSCIILCTRIYKQLKIIYKQIPDTQDMIIYSINIPCFTGWFILVRSLVFCKKFRFWRNLTFVLLYIQTIYGLIKFPFYQIYDVTIYKMYSLESYLYCDTAILVGLMFWQFIEQFARVIHQVKRRYLAYQFEDHRQNIILPIEIQGQSDEFGRIQQQQIIDNLVENAQEEYGECCFCLEKISKGQRVHKLKCHASHVFHYYCMSKWLANHNNCPLCQQTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MYM8</accession>
    <name>A0A8S1MYM8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0720003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MYM8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47112">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47112:SF1">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="54"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="316" mass="37730" checksum="2ED2BC68ADBE2D4E" modified="2022-10-12" version="1">MINQTPSTIDSSEILFDEEQQYQSYIRQQSTMEKKLQYSISANNQMKGINQQDIYNKEGWLMKKSPKLLVGWQVNCQYITKIYQKRYIKIQEGKMQYFKGSEKFKGCIDFHLITVTIIPIMKNNVIHEIRQFFGSMIIRLELNGIKKIFQFKSIDRKDLQDWYSCLKQHILMTQSYPKMTPLNHESMWRFERISETYFRKKADTGDIHLFRGQSPLSKIYRAFTGDNFDHVALLLRYNKGELFLLESMGQTGVNLLSWDTFMQSNWHNLYQQMVYKQLEVNKSMKYLKNLNNLLKYLLSKISNVTKLIDQTIYKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2C3</accession>
    <name>A0A8S1N2C3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0750123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2C3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="38397" checksum="B84939CA8FB5001C" modified="2022-10-12" version="1">MTQQIRLKLEAIKNQDGKVQNFQRVRNKRLQKCNLGSVAGKLVKEYGKCNYKNITKVLMNSQERSLCFLGRYSTSVINVGQRQLPPFDFGIDDINDQFVHQKVLKAPSKLENGAMYFGEWLNDLRHGKGILICEDGSKYEGYFQQGNAHGRGRLIHSNGEVYEGQWENDEAHGLGTYIHEDGATYTGLWEHDLQHGKGCEKWPDGSYFEGFYKHGKKEGLGKFLWIDGAKYEGEFKGNNIDGYGKYSWPDNKYYQGYWKNNKKNGKGKYVWPDGKIYEGDFVNDQKHGQGILKFPDGRTYEGEWQNDKQHGKALLRLPNGKISNGEWKNGIRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2Y8</accession>
    <name>A0A8S1N2Y8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0760072</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2Y8"/>
    <dbReference type="OMA" id="VWPAYPE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19519">
        <property type="entry name" value="RecA-like_CDC48_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19528">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000004">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000001">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000003">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000048">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="6">
        <location>
            <begin position="42"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="5">
        <location>
            <begin position="141"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="252"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="525"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="782"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8086232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="820" mass="90762" checksum="D2129FCF11CF2392" modified="2022-10-12" version="1">MDPKNKPQPNQTNQSNQSNQQGQSEKKDVSTAILDRKKAPNRLIAEEALQDDNTVIQLSQAKMNELKLFKGAPVLLKGKKRKETVAVPIPDKLDNEKIRLNKVIRKNLRIKLGDIVTIKPLDQVPTLTKVHVLPFDDSIEGIKGDLAQTYLIPYFKDAYRPVKKGDYFICRGGFKAVEFKIIATEPGEIGIVGPTTTLFTEGEPVKREDEEKLDEVGYDDVGGCRKQMAQIREMIELPLRHPQLFKTLGVKPPRGVLLYGPPGSGKTLIARAVANETGAFFFLINGPEIMSKMAGEAEGNLRKAFEEAEKNSPAIIFIDEIDSIAPKREKVSGEVERRVVSQLLTLMDGLKGRGQVIVIGATNRPNSIDAALRRFGRFDREIDIGVPDEVGRMEILRIHTKNMKLAEDVDLAAIAKDTHGFVGADMAALCTESALQCIREKMDVIDLEDDKLDAAVLEAMAVTQEHFKFAMGQVNPSSLRETVVEVPNVKWEDIGGLEEVKKQLQEMILFPIEHPEKFHKFGMQPSKGVLFYGPPGCGKTLLAKAVASECSANFISIKGPELLTMWFGESESNVREVFDKARQASPCVLFFDELDSIAVQRGSSAGDAGGAGDRVINQLLTEMDGVSAKKSVFFIGATNRPEILDEAIIRPGRLDQLIYIPLPDEPSRLNVFQANLRKTPVASNVDLAYLAKITDGFSGADITEICQRAAKAAVRDAIEAEARQKQALQMAPNKASQLIKADPVPDLNRKHFEEALRHARKSVTNIDLQKFEEFKKKFDPSFSKGSNQGGFSFKWPEAGGQQFARGQQSRIQEDDDLYGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N3C5</accession>
    <name>A0A8S1N3C5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0710228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N3C5"/>
    <dbReference type="OMA" id="SGQMNIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8084203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="117" mass="13829" checksum="A9C81036DDBC128C" modified="2022-10-12" version="1">MNFNKNRRVTLFQILGRDRVNKKSQSQHKKSNTIDHVQLPQLYDNFLTEHRIRLGQQKQFKSIKIKDDFPANTSLNVSVRSPIRLQSILQSGQMNIKIDQLLNRKKYVKSSIQMKLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4P5</accession>
    <name>A0A8S1N4P5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0670025</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4P5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="12">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="169"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="508"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="464"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="464"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="537"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8082024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="819" mass="94659" checksum="03241F72C8EBA097" modified="2022-10-12" version="1">MKIIIRYCSQWGNMSTKTCKVNPNIKVFQLKQQIAQLTDIREDDQILILNEYTLEDDRHLFSYNPEDCQFIYVNRENKVVEEEVPETEVLEVQKGYNRMKGYEKLGIVHEDSEEEDENKKKSTTIHKSQMDIAAEFIKLVKCNDIPAVKAMVSNHKSPSFDIINDRQYCGFSGFHYANQFGHEELVVYFIDEGADINFCTKDRQSPLQLALKNSKIECAKLLLSKQVCDVNYCSQGKSALQLAVQKGELDIVELILKHPQFDVQAIDLNEIKGDLYATQLVTYHCQQKLNKTQELVKPKAQKGYIKKVNVMKFFHYKRYLTLDPEAGTLCRYKTERDCPLNPIEMIALSNVVSVWNPKREWYMKGDHEYLSVQYKKSGILILQFCSKSKNTILSWKKSIEDSILYYKETERRIAQLSVQGDIMALNQYIVSGGGGQFNIIADSRCQSLNDQVAQNLQKEAQEVQEQQQKQQQQQVQQQQQSPIKTLSPIDDNHNHGNNQQYKKYLINYTIIKQVGEGAFGKVFLVKYNPTNQVYAMKQLNKRKLLLKKQVKFAVTECEILKQVDSPYIVNLFQSFQTLNNLYLVMDYCGGGDLSFHLYKHKTFKESICKIIIRQLMKAVEYLHSKDILYRDLKPENVLLDNENKIKLVDFGLSKQIENGKARTFCGSPAYLAPEVLSQKGAVQATDVYGIGTVLYELLVGEPPYFNEDLDTLYNNIRNDNLEIPSRLSKACQSLLQSLLQKEANKRIGCKIPSQVNWSIIKQHEWLDWNNPEEKVNFGQTQENLVNVMKNSTHNIGDSDYSEENYKLNRIVGWTFARVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6Q0</accession>
    <name>A0A8S1N6Q0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0680117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NEAILYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1083"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1277"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1392"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1592"/>
            <end position="1614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1656" mass="198741" checksum="33CA63F5A3114D6E" modified="2022-10-12" version="1">MNPFFLFTFEIRQEIVSKYVNSIAYIIIHYFIILINHYHKQVKKQFQLLSYMIIAQTRGSNNSNLSSLFNQLDVSNIIFQYKELTSIISITEFALSLFITILIIILLFKRNNNKAEQIQFSSLGNIFIRSIRILYWILCYPLFLVSTYNVMIAYDFSQFLSMFVLIFVIFFTFFHDFVLLNDNFNKQDFLNIAQSRPYILNSWIKLVQCGFSSYYYKNQNSHMIYLLVSFTLSILNYIQLQYYMVIFGKYDIDYIFHFVNLVYFGQSLGLLIQYFINIEDPYTLITILIIINLVVIHKSFRKWYFEHQFTNSKDIKIRVLLLSWFRELECSNVYYMGIITKHYNHPNNKKCFLRHKAIYYSGKKFAKYTYITTKKHNIEKYKGLFLKFFIKCLLETQLKQEPNSSELRLLYSEILFYKFNLINESFKQLLKIKSNFNFNQQMRIIQLKLSIHNKLKENNQLCYRSKLPFENMLKCEEQMKDFLKAFLKITELQIFFWKNQLQQFISISEQINISQQIFKEIQVCQTNWKSIHFTNPIDEQKMFIRFRWKFFYLYFKLYILHKKLKVHELKELPDQNLKSQQIFQDEKIDERSSSEDETQTKTYYQTNCIISSNNLFFRVDKYGEIITASQSSLQILGRHQNEYRGVRVETLMPDIIKENHNDFLKLFYKTGQSENLFKRRSILIKHSKGHCFKAQKYLKYIFNLEQNRFEYLSMLRTVSSRSQYIILNSKWEIDSSSEFIYSILGDYKLPFLSLCPKAISYTEFAQFLTTYDMKILSLKLCSFGSRERIQDKKFTFRRQHDQTFSNKSINEITSLVFKRELEEKQKQNVIQLFQQVLDEDNEIEGTTKLTKLNHIKLNIRIPIYKSEFQEDYNKYDAQMKNIFYNGESQKRLVFRNGNGKIRVDKFEFLQRYRWVKQQRMMFIYSKIKYLFEHYCQESILEKVLKVDANIKFYKTLNKISFYILKINRLELYDENTNNNNSDKQEILFSNTNSFKPYTSTIVREVVLVTQTQTNTLKNQSGYQYSDMELISNREYTLKPFLIGHALREDFLVQGSNTIYSLNEQNNPFIKEEINKISKRNQKLTLLVILNRFLFFFELLLISFTYFFCSFYYNPLTLHNSVTLLGHIFKISLITVQSYNYIIDVALMNENKLTSLLIINPTTKFNTTDQFLEFVHTDIDEQYTFIYNIYTNQEFVSMVYSGFYIYDKNVSGLDIFDQFQLNLLQFNFSDKALINLNNTILSHFRDYMAPYGQQLMEDSIISRINEAILYQTQSIDNLILFMIFQFSLMIIDSMLSLLIKFNFRNDVNTIFNIVANIPKEDKIKAIKQYQTLFSQLNSLFEEEQYGYKKSSIQALNADTVIQKDFEMNEVEKANQKRDKVKIKKLSKYQLNTIIALICYLAIILVLFILLYYFILIQQIETKQFFMDNNYLAQTNYFWIITLLKEKFIFENYLNSSYYPNITKLRFQEFLNDMSVISPLLYQTTISDIQRIFDDTLCDYSFDETVTYIYYDQFCQEILDGALKRGLKQFNILLSQVVIKALNPNDQRYDDFQIEDLAQYQISFQIIYEIFLTTFQIWAQNAENLLNNTLDLSLVSYIIQLLVIIILYFSLLEYFLFTKLQQDFDFCKNYYKYFMLNQTMNQVKTMRVDVIKTGLLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N814</accession>
    <name>A0A8S1N814_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0820092</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N814"/>
    <dbReference type="OMA" id="PFIPRSY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="282"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="496"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="50"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="362"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="383"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="458"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="601" mass="71192" checksum="386A9F50B88C8CB3" modified="2022-10-12" version="1">MSQLPQYTSQTVESQYFQQPSQMSIKPQPITFYPPTFTQLQGPLASDQNYQIQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLQQQQQQPQYQYDDQYIRSRQLPQNLQQQMMQQQWNEQFQQMLQMHHHNYLLQNQLHNLMINQTAQSEQARQDLQIQQQQSMADFTNLQPETITEYIPVEKKVIEYETRKKKIQVPVTHEVTEYVPVTTETISPERTVRLSPPKEVHMVPTVVQQPPILQQTVIPRPPQLQMVHAPTQMTRMPTVPAYQQAPLTSVRSQLQQSPPRILNKPTEIPIPPPPPHLPLQPFNYTPKDFPTMNMPPPPHQFQGQISLNSPQPPPPPQFDSHQIQGQISLNSPLPPPPPQFDSPLPPYSHQFGHPQNQLPPHLLPQVPQHQFNQHLPHQQQHQINPHQPQHQQQPHQSQQPQQIHQLQYPQQLQSPHQPHQPQYQQQPHQPPLLPHPPQSRPAPFIPEQYYRDQIEDEQEQHYYDIPQRKHPNHPDFQQTSKLDTPNYHIPNYDSENIRDKQSEPIQYPKRAPFIPRSYDSDLHYQKPEQIDIPSKDNQKRGIQRSVYEKRPQNSQQTLRSKENYFKDSIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N8L0</accession>
    <name>A0A8S1N8L0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0800222</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N8L0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="21"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8088372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15721" checksum="6CA2C4D949CA69BA" modified="2022-10-12" version="1">MRWQQNSTITMIKHTIIEAYIKAQYYIGTTLLKLNRIEDALQSFNTAIDLNKNNDEALENKALSLHLLQRFNEALKIYDQAIFLKKNSNRLKRKADTLLVLGRKNEAKQYYFEALRIGNLSSDQEQILYQLARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N962</accession>
    <name>A0A8S1N962_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0810215</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N962"/>
    <dbReference type="OMA" id="RLYIFHN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052981">
        <property type="entry name" value="Ingression_C2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052">
        <property type="entry name" value="CONSERVED SERINE PROLINE-RICH PROTEIN (AFU_ORTHOLOGUE AFUA_2G01790)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052:SF3">
        <property type="entry name" value="INGRESSION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="146"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="4">
        <location>
            <begin position="476"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="304"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8088612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="67012" checksum="6D62E28AAB6DA540" modified="2022-10-12" version="1">MGCGSSAGAVIEQNRAPGFKGPVFMTIKHALLERDVRFIGVMDPYIKLIMGKQEFKTPVAKEGGKHPKWEGCTHTFQKLLGEPDVIIGQVWCENTITSDILIGEGAFSLTSIRKGNAEPKRISFLLFHHGEKAGEISLEATFTLDPSGKDLPEIKAPDTEGKFIVKPKFGRLKKNANLLLKMDPFLTIHFGNETMNTSVDAAGHTTPKWNDEIIFTRETNEDTLYIEVWAESLIGWNDFLGCGYASITDSLALKENQLQTVTLFYDGANVGEVEIECTFKTAKPQDYLQKDKQSTEAPTKESFQQQLQTQYQQPQQQQPQQQQPQQQQVFPQQLNQFVQNYEARYQNSYGQQPMNGRVDFTYPNGDHYSGDMMNNLKHGQGRLQFSYGGYYEGQWANDQYNGMGVLVVGDSRYEGQFINGKKNGQGKQVWNNRQQYDGQWVDNNMHGNGEWTFVNGAKKKGVWAHGNRLRWLDDDQNIIQAGKTMTSGFMLTSPNQQFTLSFFHDGRLYIFHNQTRQPIFSAINFNRQPLAPVWLKFDPSGELQMIDAKGVPYWVSGQNFGIFAPPFVLILQDDGRLVIQDANGQAKWYSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9V2</accession>
    <name>A0A8S1N9V2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0830188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9V2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035921910" description="Beta/gamma crystallin 'Greek key' domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="2">
        <location>
            <begin position="948"/>
            <end position="1024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8089428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1036" mass="115410" checksum="926318141DBCA1A6" modified="2022-10-12" version="1">MKNRTVVLLVIIYSAISVLGEPVVVSQSFQGNGFNDADGWVVAGGLAHVTECSGTKMFGGFGKFGGKAVASKLFELPPHSSINLKVQFWKIDSWDNEEGYIFVDDQLVWSRKFRHNEGDGQKCGQGGDWKEMTLNLDLNIKHTGATAVVIFTSNLNEAADNESWAIRDFVLSVERCPEDCSACQVDDKAENCQFWQSFSSSWGALDHNQLGADGWEVAGGQKSATDCGGVALFGGFDKTGSKTVVSKILKVNPHYKLKIKVLWAKIDSWDNEAAQIKVDGKMVWERRFQWHEGYFGKICGCPVFEWKTIFARTEVDIDHTGDQAKIEFTSTLNEAPNNESFGLRDLYIFYASCSENCAECTGPKDSDCKKCANNWALVDGKCQALPNFILLEQSFLEDKFTGINGWILNKNKGGRLINECSGKSMVGGFDIMGIGASATKTFDIPPHKRLRLQSTLYKIDSWDGEFMIIKVDGTDVWKTSWNLQTGGANFCGQGIWFDGIVGVDQIFNHQAPRKFRHNEGDGQKCGQGGDWKEMTLNLDLNIKHTGATAVVIFTSNLNEAADNESWAIRDFVLSVERCPEGCSACQVDDKTENCQFWQSFSNSWGALDPNQLGADGWEVAGGQKTATNCGGVALFGGFDKTGSRAVVSKILKVNPHYKLKIKVLWAKIDSWDNEAAQIRVDGKMVWERRFQWHEGYFGKICGCPVFEWKTMFARTEVDIDHTGDQARVEFTTTLDEAPNNESFGLRDLYIFYASCSENCAECTGPKDSDCKKCANNWALVDGKCQALPNFILLEQSFLEDKFTGINGWILNKNKGGRLINECSGKSMVGGFDIMGIGASATKTFDIPPHKRLRLQSTLYKIDSWDGEFMIIKVDGTDVWKTSWNLQTGGANFCGQGIWFDGIVGVDQIFNHQAPKAEVVFTSTLNQDAADESWGFRDFKLWYEPKEACAVFYSECDYKGASFEFCSKSPNFQKDNIPPQIRSIKIPPQGRVTLYESTDYNGKKVTYTSDQACIQNFDFSLIQMSGHVEGGWIEVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBS5</accession>
    <name>A0A8S1NBS5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0760224</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBS5"/>
    <dbReference type="OMA" id="MLINSIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="129"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8086465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="214" mass="24692" checksum="229FD8AD3035FEB2" modified="2022-10-12" version="1">MAYALAKWQTSSTIGVISLVPNDNQVVFDLQNALQFLKNENISQFQKIKYITTQGGKWYYLPFNKEVVQLVLTANDYNQENVEMLINSINAKIFDHNPITPQNITIQQKQNIYNLLIYFDQAHGKEPKQIQQILQTLDNTKQTVQQNIEKLINNKEQLLNIEVQSLELDNSSQVFLNSAQELHRKQRMQKLKTRLIIGSIIIVGLAISLYVIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCN8</accession>
    <name>A0A8S1NCN8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0790118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCN8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="131" mass="15349" checksum="EDAE2C2165EA2EEE" modified="2022-10-12" version="1">MLKKKNNKPTINGQSETLNNCRDKDIQYYIYYALIVERIKDNPLQCLLNSKPKYKRLGTLELIGYRNLEIYQSQSESKEGKILLKKASCEVSNWIEVVMNFLLHSTSYFKGQFTKVVNETFCIITKIKNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCN9</accession>
    <name>A0A8S1NCN9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0820190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8089019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCN9"/>
    <dbReference type="OMA" id="SHVCQKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="196" mass="22842" checksum="B485EE7EBD1F10CE" modified="2022-10-12" version="1">MTHIRPPVPKFNDECLTPKMEMSLLEEVQHLIFKMNLDFEIEKKATKIIQIIPLQNTLTSAKAVIFFCLKELNRKLPPIEKKVEYVIKYIEQQQNLNFSHICQKMGFGDQVSKVCETLKKQLVYLIGKLNINIQIAITIKIAADIIFYKQGGLNTKFLAFHTKVNEEQLKCSLNRIKPFSEKIMTELFNHYNDNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NER6</accession>
    <name>A0A8S1NER6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0880145</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NER6"/>
    <dbReference type="OMA" id="IETQPNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000121">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="366"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8091587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="565" mass="66497" checksum="21F7359991913C24" modified="2022-10-12" version="1">MYKTTQNYSYADLLSRQGISDNGRSIITIDKENSYALLQKKKQLQNRENSSSSQQKNENRSNSGLQKSASLQQFYRAQSAVEKNSYQNQKGNQQQIKEMKNVSYLFLLQQSGYKLRHNQTKSTKEQNSKGIEFLQNDYYKQKPVENQAYKRLNEKLEYLEKKFNQLKDSFVNISENQQDKKSDRIQQKQYSLVSKYFPKKFIEQQKNNSQEERKVTKDIKSIFNIYSQIKDIKSSSSNSQLLKQTQEKKNVQLQDFVSQIKRDFNNKNSQLLKGSYVHQTNTNLNKSERQSSMDAKYYYFNKLIETQPNEPFLYYISTVLTAIKNKKPTEIDELIKDHFSQTYQGLIYASRLVIQFDPDKVINLPRSNNLKTIVFDLDETLIHCNSNVSIPGDIILPITFPNDEIVQASINIRPYAKQILQTLSKHFEIIVFTASHSSYANIVIDYLDPKKQWISHRFYRENCLQTTEGAYIKDLRVLGNRKLSNVLLIDNASYSFSKQIENGIPIIAYYDNKEDQELLHLENYLLNFRHVKDVRDLNQKQLKLKQFLDYSDFDDLYNNLHGMYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJN8</accession>
    <name>A0A8S1NJN8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0880146</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJN8"/>
    <dbReference type="OMA" id="WVIVQYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF23">
        <property type="entry name" value="MINOR HISTOCOMPATIBILITY ANTIGEN H13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035886029" description="Transmembrane protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="413"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="662"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="737"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="738"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="784"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="795"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8091589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="828" mass="97168" checksum="4BD4DF1C550E03C8" modified="2022-10-12" version="1">MFIYILLTITLAALPNANLSIGPLLQSFTDDEDLDQIVYVHQKVYENFDYASFPSLSCCPNNVTKFRVMNLNKQGSNIQRFNLPFYIENLGEEPLLIEWLNITEEISDQDIQVQINYQRDLPIIKGFDNYLGLNLTHLCWKTLNNDRYWSMKRVTLQFEGYDPVIFNYQFLCGKDYYPVRFDWSNIILIIFESLMILVLSMFGKIYAIKVVFITKQLKKELPQEKLDKIAQESSPFQGFLLGWSQALFYMSLLIGALFISLYSQEKAEKPIQIIVYILCIICSIHFIDELFCAFRKKVPFFVKSFYFIRYCDIFAIILGAALFAIYLATEQIWILSNLISICILGSLIKLFKITSLKDCLLFFLPIMAMDIFCSIYLALNIRYEWDSLILRYFNTPLSAQIPYFRHIYKKKCAWVSIFNILFPGFFLAYVHRFDRLKKTFVYEIVSFFGLLIGLILWVIVQYLISFPLPTSIFTEVLMVSACTLIAFRRNELNLFWSGNFYDEILLDPFKNELDKKSVKLISFFNIGVSQDLKKKYALNSGAEQTANVYEPFELVKANQSGELKLDSILFEGLQSFQEIYSSPNQTYRNNDQNINNKQDNNIIENKKQQQEIQTDIKDQKDVVQVDSLIQNSQEYHNQENQPLINNQNKEDINIKTENQQQLKDYQNQKDQNEDNVESKTPPDVQKQKSLGKPKISFLESKKLEEVIIETPIIQSKNNEEDKKQQKIDIQIELKEQQKVSQGNQNKQSNQQNKIDQVEEKQNQQQNNKVYTSPARQKYQVNPPKAKEPQVDRFQQKQQLRQQNDNFKKNLQFFQNQEKVGNFKTPVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKC3</accession>
    <name>A0A8S1NKC3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0910011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKC3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="307" mass="36592" checksum="B739FD17F90E6D7A" modified="2022-10-12" version="1">MKRFGQSPLLGLALKNQNKIFQIMIESIFFFCCLNQNLSKQLIQEFWHWQAFLAGDNIQFKDKLLQKGVFKSLIKLSIRYKTDFVVFNTIMLAISNLARGKTNHKYRQRIVNRCLLGFELLKRRRNYGVVDKLTLLQILNILKSYYNLKKNNYIPCFEDQRKYFIWQQDIIRSYFKDWSVIKIRNITLTQKQRFEDKSKIKKQIAFYLTNSAELDDLDHLVMSHEFIKKLSNHLDSNNKTIIQGSLQGIFEFIKEVIIINHLVQYDEKFLISKKLIKVQKLQKSSIILNSFIQSIKMLIKYQAIKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKW0</accession>
    <name>A0A8S1NKW0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-hydroxyisobutyryl-CoA hydrolase</fullName>
            <ecNumber evidence="3">3.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">3-hydroxyisobutyryl-coenzyme A hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0940118</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-hydroxy-2-methylpropanoyl-CoA + H2O = 3-hydroxy-2-methylpropanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20888"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57340"/>
            <dbReference type="EC" id="3.1.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.4" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKW0"/>
    <dbReference type="OMA" id="LVWEQIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003860">
        <property type="term" value="F:3-hydroxyisobutyryl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000026">
        <property type="entry name" value="3-hydroxyisobutyryl-CoA hydrolase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045004">
        <property type="entry name" value="ECH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032259">
        <property type="entry name" value="HIBYL-CoA-H"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004127">
        <property type="entry name" value="PRK05617.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176:SF3">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16113">
        <property type="entry name" value="ECH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl-CoA hydratase/isomerase" evidence="7">
        <location>
            <begin position="28"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8093907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="365" mass="40954" checksum="C003BBDEB4B35926" modified="2022-10-12" version="1">MRLSLISAMFSSSNSQVLTTKVGSALNIKLNRPSQLNALNLPMIAELNQILDQTNQTSCIILSGEGGKAFCAGGDIKTLYFAKTQPSEQNPPSALKQFFFDEYKLDYRLATLKPVLVALMDGIVMGGGVGMSIHAPVRIATEKSVFAMPEAKLGLFTDVGGGYFLSRLPHRLGYYLGLSGFRLKGADLVHAGLADFYVQSSELQNLISKIGTIQSQNLNEVKNIVKQFQPNQIPEFTIKQQLQNIEPGYSGESLEQIFNNLQNHQNQKFGQDNLKILKDQCPLSLRIIFEQIKRGQKLDLKENLKMDYRIVRRIMQGTDFFEGVKQVLVDKNHVPNWSFKDALTIPQSEVDKYFKVLENEEELDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NM76</accession>
    <name>A0A8S1NM76_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0790065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NM76"/>
    <dbReference type="OMA" id="GWIYNDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="230" mass="27239" checksum="4A69BA24B72685AA" modified="2022-10-12" version="1">MKHIECPIKLASASVKFPNSQLFNTNKARNQILFRIYGHFIFGFTQQSPKDYYSKYSIQVEGRLLDKIQEWAEYMRYQNLPKVILNIQDQNENPIIKYFFPDDDNIGWIYNDQNIHLNIISTNLISGVCIDRSKNLEKKNYSMIQEKSNFTYKFSAKHIPEAPPSQTFMQHQIEFISNRFPQLEGNLEDGYRRHVLQIETLAGRKWFERIGDYYITDIDNCLEGNPHLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NNQ8</accession>
    <name>A0A8S1NNQ8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0970097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NNQ8"/>
    <dbReference type="OMA" id="CILGQQP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035904778" description="Trichocyst matrix protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="41689" checksum="895E94523BFD71F6" modified="2022-10-12" version="1">MKAIIFIALLACILGQQPEMLEQIAQNSFGRQILQTIQLELTQENAARQIYTMLNKLFYDLRDEDARSSKANGERQAQCSDQFTQINTIQEKALVSKADYERQIPGKQEELTNKLAQVEQKNAEIQRNDQLQINFSEQRRKEHQLYEQKRDELIGLINGLKQAQQIIRQLQTSHPGGALVQLKEHHEQLLKSYAANSEFKNMASLLMELCTDIKIHSDNDNVQVIVDIINDLIESIYDVQKREMYAEDWAQKFFEQDLLRLSKENVRLQGQIADDQVAAEFAQQRLEDLQQQALLQQIIYDNKEVERKSFEVACKEDNNAAEQARVSRNEQIQIVLQLLELFENNFNDRTRAALLQIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQE4</accession>
    <name>A0A8S1NQE4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0900206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQE4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="166" mass="19974" checksum="6E1AECC034D451BD" modified="2022-10-12" version="1">MSKINIPIKKSIVKLRNHKRLEPHRKQFLINLVIFENVSIRQAAQKLQIKYSTAKYIMKNFKNNGKQFNKNYQTHNKQNSIIYNVNIVIDVSDGSIQLAKTSKKINYCDYNSLKEESKYKNFSQTSEFIYEKLGKIFWHKFHYKEQLDEEILKLFLDRQHILMKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NT80</accession>
    <name>A0A8S1NT80_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0980184</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NT80"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8095462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="35" mass="4363" checksum="B4CBAFAD536CA6B6" modified="2022-10-12" version="1">MNQFNNLESRIVVFSLRYQKIEQEYKLRLKKCQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NV55</accession>
    <name>A0A8S1NV55_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1000108</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NV55"/>
    <dbReference type="OMA" id="YVVATFC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000457">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="23"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8096122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="456" mass="52347" checksum="970A75A1BC45B1E8" modified="2022-10-12" version="1">MNNLSFDDILSTRIKFGKVQIQTFLIISLIDLLDGSEFMFLQLLNAIIYKEWSLSLSQLIVLATVFNFGQFIGSLICGQFSDYTGRKSMLVWSCLLQVLKISMLLTAFVQDLPQLIILRFCFGLLFGIALPISSILMAEVTPLHVRGQFIVTLQMMYIVGRMWMLLLAFIFLDSIATGNWRAIAVTNSIPCFICLIGSILYLHESPRFLISQGNIKEGIEGINFMGRFNDKDYVDLQEDEIQALQQWRKATFEQQYDKKSFKELFNQENLPITWRAYSLSIISMLMYSGLYIIIPFLFDEEEKTLLDLLYTVLIELPAVLVVVFLIDKIGRLPIVLIGTVTSMIAVFIIWYWRARYLLLGLIAFKFFNRMTFLSFTPLILESYSTIYRSLGIGTTIAFGRAAGFLSPAIILPLYQKDNYSPFLASIFILIIMTIIFATYPKDLTRKPLDIKYEKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXF4</accession>
    <name>A0A8S1NXF4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0970144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8095011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="QECNNEK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="247"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="356"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="988"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1052"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1239"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1352"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1647"/>
            <end position="1688"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1717"/>
            <end position="1939"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1963" mass="234998" checksum="32D1B1A48F862233" modified="2022-10-12" version="1">MNYQIKGNFNYHLEIQASQLNETINITINAFSSKINQIPLGCKFQWFKKLDNERIKIETQGNIYPCSIFDIGYKIEAIVQPFESGYEGQATLEFQKIQISTQLENKLSQLYHNNMKQQIICNDQNWIFSLEYLENSTTQQKIEYADQYILPNQNQLQVQFNNQIMKFKCKDDKDAFCAFFISMQCLKRVHLKFIAYNIGKLNQKQVNFQQLLNAQLLQLINEKSVMPIKKFEPIQSNIMMATSQDFRQNNQIQINQLKQELIQLQNEKQKFQQQIDKLMKDNKELEMNSRSNVATKDQLDSMDRLIQSLKNEVYTLKQRETDLMNQNGRLTMALNEKNDKCDKSLSQSQFLDELKLKQYQEKLQECNNEKLKLQEELNKQQKFGQSSRTITQNNDTEKERLLQVNQKLMQEIATSTNKIKELQLELEMMKNMSRISMSMSMMEDPVMKTKIEQLEQENQYLQKKVSILEEELSKKKSTKPQKQQQDRDLQIQKLTEANKRYLEENLKLFEEIRQLREKFDYSSILQGTMKDSQIQNNVVNNQLEQQIERMQQIHNLEIQKMKKKIEKLTNDLQDSNQIKKQFEQLIKQNKRLVEENTHLTEQIRNLADINSSKSFTDNMINSKIFNCDQYKELEQQCIIFKKKNEEIQSKLDIQLQKDIQQLDKIKILEQEIIKYKNQIREIENNNQDNNQIKLLQNQIDDYQKTKDNLMRENIQLRDDYSTLMQDKEYLNGKIKQLENSIFELKQKQMQLQEENRTLKLFPQSAERQFSIQIQQLQQQNKNLTEEIVRLQTQQNAFKDLNQSNLSISESVLGSKVYEAKEYKLLENQKEMLFNENQKLKLEIRQEQLNQDNQIKFQEQIKSLQNENDLLNQQLISNKEQHSRQILILQNDIDKLNQQLQNISINENRYKVIINDNKLEDNNQKYQQDILKLENENRLLNQQIQQLQLQYDTEIRKQQIILSDLQFENNKLTREISYSKQYSESIIINQNEKKQYQSQESEIAQLKNQLAEKTHQLQIIQTNSQNNMIELQQVISKLGQENKNINEVHNSIKSRLETQINKLIEQLKEKDLQINRISEELRKKDQVYKQQENDLFMKSNQIEQLIQQNKGLALQLNNLNFELQELKMTNLSHSFSKSHIDVRENENQELQNEIQSLRIQIKSFEQTQYQQQQIIKQQENVKLMQLEDEILHYQKEIQQIKGKLQITMQDSEFYKHEFIQSQQQVEKIRKEQTQKQYEKLSILENELIQIKEIKQELTQENIELSDIIQKQQKQMEDLQLDLDEQIENNKSITKLKMQLQLECEKLQRENQQLNNKIQELLNIQQINRDKEFNEQLRKYQQIEQDKIKLSIQCEQQQKVIDNLKSNFDIQQNKKDEFEIKLHTSEQYKQELQILLKEKDQQINQLQEQSQYLSKELDNEQQKNVSQKKKFNDLTLQIQNKDNQISQQNLKFKNLDDQIQKKEQQINNLNNQISQLNQQINKYQNEIIPELNQKLNQESLEKNQLNQQIDCLQEQFAQMEQQILSISREKSPVRSKNNSLCKPFDQGLNEKIIQQEQTINQKNQLIEKQLEQIKTLTKQNDGLLQQINKLYEDLKEQQDKSFSLDRSSETYLINKSFNSKYNIDNELILQHQYQSHDIYDSDKKRQSQYQQFEQTFQEIVQENKIQKQEIDQLKQVIQKQKQEYEQQIQMIPISNNLSNSEIHNLDQDNIKSRSLPCQNQSIKNELQQQINRLEEELMMKQGLEQSNQRLNNEIRLQITKVIDLQQQNEQLSEYNNQLNQKIQEKDEKIKNLQQLIIQNSDSNQVQKEEEFINVKQQLQDDILRLKKQNQQYEQQLSEYQSKIETLENEILRKSQQLSIKDSQVNQNNRKNKKLEEQIQILQDQLKNQGGLVTQNKDELICQLQNDLEQNKILIMDQQIQIKQLEEQIDRLTKSNSALMEENCRLNEQVKDNVNVSYLSNDSQNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P248</accession>
    <name>A0A8S1P248_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1030139</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="IANAICN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="4">
        <location>
            <begin position="3818"/>
            <end position="4144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="41"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1831"/>
            <end position="1856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1831"/>
            <end position="1843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1844"/>
            <end position="1854"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="2">
        <location>
            <position position="4111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8097253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="4144" mass="490008" checksum="0D3199F0EE79B53F" modified="2022-10-12" version="1">[encoded data, 4144 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P368</accession>
    <name>A0A8S1P368_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1040042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P368"/>
    <dbReference type="OMA" id="CCKDVIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="496" mass="59131" checksum="ABB11E8C5572D18F" modified="2022-10-12" version="1">MSWYTEIADNLESYQKIVNEKIKSTKDFISLLQSIFQLEESYSKSLETIGLQISKLINDKDRTTELFTILRSYLLIKSEQSKCFAMQLNNTLICEQQEYLIKQDNYLKELLQFNLKNKKEYQQNFQTLNQFTIEYKNAIEEEKQYQLNRLQKKKLSIAVSELSFQNFITIYNQYIDACCKDVINFQQNLQNLELERKNLLQDSHMKFIIFEISVIRNLQYDLTGISQKLEQYQPTDLKQQQQKNIIIDKIDFEVIKRDIYQQLNFKPDSQILNKVKKPIFDQVKSKDSDNNEFISEESLNYQKIFQNVLLNNSVTDEIIKKLSELLEIALPYNYTQCLEQVLQKVISEKQQKTIQQYQVSHEVYLSIQKLLITLLDHCNKYQDYATVILEACLFAKKIFKRVYLPTENKEIKLNVFEGLMNHEVIQNSQIWIDQAMNMKKQPTQKLYVFKENYNKNSQIKYQNDEYINALQQIGCQKQIIENIRILLNKDNQNVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P546</accession>
    <name>A0A8S1P546_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1060059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P546"/>
    <dbReference type="OMA" id="IHYYDQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="251" mass="29519" checksum="DAC007E13A74E356" modified="2022-10-12" version="1">MKSKLQNGNYFLNKDFAFYHRPQIPVTIQSKSALEIKSIPQPTNLQKKQKTIHYYDQYKFEQYTSAKTKLYDLLSLRQTNRTPIQRRRQPESSLIVKRSPEIFTSIIEINDVKQKVKTVSQECPRTQQKITMVQHSSQLVSTQKRNHNHSKSTNNNNTSTPILNKTVSQFQCFTPLTNQKNNAQSDLIQRIKQQKLESLINLIINNQLNNIVNAFTLIKNTAPLLPYFKELERRKAEFKQKKFQIKLIRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6A3</accession>
    <name>A0A8S1P6A3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1070121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6A3"/>
    <dbReference type="OMA" id="YDIPIFN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="90"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="354" mass="42913" checksum="7620FBFAD7987C5D" modified="2022-10-12" version="1">MRNAYDQSQCQQVLKVYNNSQGMLNQNNYFLEYDIPIFNKIPNTKKIQTKPKENIFYHKTNYKDKNIVEINDSNSTPLYCIDWLKSKYQNEDFDHLKKQYEKLLLIKPEQYIDYQIEKDLKRTLLAENKIKEIKNILLAYSIFDPSIGYIQGMNYIVSVLYNHAKQEWIGFWLFVLLIEQLEIRDMYQMSQNSLNKYSQILDFYICRCQYKLYINLKDKQVQTQLYIQQWILSLLLQQIPFEYSQMYLDGLFNKGLSYFYTVAITFIKMFEQPIIEEEQIEILMILTQKQNVLNYKMQKLLNWPFILSIDWDIKKQDIRAIIESFDSEQNIFQISRLQTHQSQQITHFIQFIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PDY3</accession>
    <name>A0A8S1PDY3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1150039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PDY3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="48" mass="5426" checksum="DBF4ED864B0BEF8C" modified="2022-10-12" version="1">MQKMKTGMNGSDKTKCKPQNPQQQPNTQQAQEVINQAVNNPQNSQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJG9</accession>
    <name>A0A8S1PJG9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1190164</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NWKERKE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:002029">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057978">
        <property type="entry name" value="TPR_DAAF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25757">
        <property type="entry name" value="TPR_DNAAF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="123"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="377"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="552"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="669"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="946"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1039"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="613"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1176"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1210"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1496"/>
            <end position="1531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="620"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1213"/>
            <end position="1232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8102933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2033" mass="234183" checksum="920108B0715C5F2E" modified="2022-10-12" version="1">MSEEQVNENKESPEIQKPVDVQEDPPVEPKITQSTYGNDDVPIGKSQNKQMLEEFPPEGFVPKKPAPKKATKKPVPPPVENDEPKVIINPDDIPIKQAGANVIDDQPVGGGKKFAISEYPEGMEGGGEDLQQTVLPLGQRLKSKAWKLRQSAFEEMAELFQKDESLDEYYDEWPKLINDNNPGSQEKALIALQIFIAKCNKSQFAARFDAKETIRILIDKCIAPGKKQIVKLSYELLFDIFERRDKQEMFDVINEMLKNKVQKVQCAAIQSLVELLLTFGPKRLDYLKPFFPEIEKLSQSTVSSIKTECMNFYKEAHKWLGEPILAPFIKGLKKLQLEELEKFQKEWQPVPMVPTRGGDAVTVGQGGGNNNGLDVYDLVDAVDIFNKHNEKWCDKVLAQTKWNEKQVLLDQLLKEAATPKIAPGNYVPMISMIKKLLVDSNQVINVCAIKLCGAFVKGLRKNFTAQAKLLFPLLIVKLREKKNICQEARAAMELFHYSLQVEDVVEDFKEGLADKNPQMRAQCLLLFAKLVQLKGFPQGAPKPKFVDCVKQLLPLAKKLIEDSVPEVREASVQSLGTLKPYLSDQLINSFYSDIAQQKLNKINEISSQIEEEKKKEQIKKQPQQTKEQLQQTSQIQQSNIQNNSIQQGTAKKPPLSQKSNIVITEQPEQSVDQLEGLLRSYGIDDQFFENINGMAKLKAEAIKMLENETDAMQNHFEDILNFLRFKLKDWKEANLSINKELFSILIYANNLDPKPFTQKSFQPLCKLLVEKMTDSKFREDIYIILKGCCECVNPKYIVIYLITQATPKDDKESGKPTIMPPQKIGEIINNISKIIDIVTIKNVPTKEIIDFGKEQLQSTNAVIKTSCVELFKTIYKYIGQELLQFIQDVQPPVMKTLNSEFDKISILSSYEPIITFTGDAAKECGVIQSQPLSQSIMQSTVSSINDNLPRADISNQLQSVLKKMADIQWNKRKEGMEELDKLLTHNNNRIQINGLNDLINMLKQRLSDNNKQLVRPIVQIVGRVAEACGKDFKSSGKQLISPLISILSDKQVLVRQDVVASLDKFCIAIGIDQVILQMPSYLQMESIELKQEVLLFIMKYIEALLKIDYKPFILPLINCLCDRVKEVRQAAEQVCERMVEDVGIDPFLNQMKDLKPAVVQQVKQFFSKFGMTEFEDTSKRTGKTPKGKQQSQIIQQQDIRNMKRNLTIKDLQNDQNTSQEPKQRNEITPNKSLHQKSHKTLPQDNTPLRQQKEKAIFQDNNSLIAKSSSIKELKQLRLQSDQIQSWANDCFPISTFSDLYVCLKEDLYNNLMSYNYKDVMKGLQQLMTVWNDPEQKSDIIELSDLLLKFVLVKIYGCSANQLLINALISFLDAFFQTISKQRYILNEVEQIVTISLLRELIYAGVDVHPELIFNLQCVFPVEIMIKKLLQLFRINPQGLHPFLIDQKDRIDTQLSNLIIQGQLVKFFPLDLFTEYSQYITKELQNELIALFGENTIRLQNKQYQSYQQQQQQQQQQQQQQQQQQQQQQQQQYQTGKQPIVQGTIQLQSVKDPRSQYFLQILDSLLSQQTSQNIESLMQISDLLTQNIQQNQQLFVDNAEAICKCFQTLLQNTFTQRQIYPAQFIQFFLQALNKLYQLKPYVNQLPYELLCGFTEEIMLRLLLEDEIKTQQENGDAIKQLNSTILRILENTSQDIMFSILFDILTKHRRRSNAQKMIDLLVKCIARLTVKIEQNSNIKIELLLLKFHNYLNEFSLHPNFALDEKGVKTIKVVLQQLVKVRGERIWENYHLVSRSTQQDQYLNKWIQAYLGNPSLQNTQQENNLPDQELLQIVEMLRQPTLLDSGIQKMVEKIKRNPTIQWQKYVHDQQIQLLIQQQLTNGQTTIQGNYMSNQSSLQYSQLQQLPLQQSQLQQSQLQQSQLSVRPQLQQQQQQQQQQQILQQLPSYPRTTRVADQQQQQQNDSFGGQQFNQYLSVNQYQMNQNQLGQSQQQNVKTPEYLMQKLTDMKNKVQTLVGPNNVQQAQNPRQLQNNRMPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PM14</accession>
    <name>A0A8S1PM14_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1220126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PM14"/>
    <dbReference type="OMA" id="YDEKWIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="188" mass="22442" checksum="5604A82A5A76D471" modified="2022-10-12" version="1">MLQEKILTMNINSSTVAFINVDYLISDEKEINQDQLKNFIEDKKINLIITTRNKEELRFLRNIHIIKIDQKEDEESIYKANIEIQKHKGNFFYVLDNYIAMLKYIICLKITYNYISKTENLDKETKRIIQGFGLQSFFQSYQIYLSNIYLSNESTNISNIQIVQEQYKLPAPISIPEFKVEYDEKWIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PNG9</accession>
    <name>A0A8S1PNG9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1240053</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="WATENNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035945321" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1433"/>
            <end position="1455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8104531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1468" mass="167954" checksum="9DB157625121FE4D" modified="2022-10-12" version="1">MTQFICVTILSAFFALLGQASECESLIQEFTIIGTVNESLSWDLRKNFFRGSDLKFNVSLPGRPYMDTYEPIQQMGFGTNHGDEDIGVILTLKPRVNQETRAWMNDFIFLEQNETDVDIHYSINDPMNTRQTPYFSDFITVHKSDALVCFDADFVTETTIIVDCTTALAGIANGFIFIDVKTGEKHIQNNDNPHNYNKTKARRIATLNYTAKSSDGFAAPAQSYLFRGEPAWATENNTQDKLDKDCELEVFLIDIKSEADQKYVAMLNVTILKTLLPEPVENFSLIDFQVEPNGELYVLDAFNGVYVLELTNTNQFKLLQHFENPSKEQIYAFDFNYLGNPDGTYTQHLVLAYKNKVVVFENFSQKFTFQLPHQAVYGELKLSMSQEFLVIVQKQGTYLYHIQETMLLFKNEEYFEQVLVNPYYPDMIGINQQRAYRYGLSKGYLQMFSQKEPTATQIQFNLNSWPQNGGKMCSILIKCTILQQNDTNIYRTSNDPFPDQIVYPSKSIVVDLLASGPSLMYKQLVNNSNVDVTLDSMWEVFVKGVDLPDAKDVVYADLLVLDQGDRVYFLFQTPDQNCTMIRCSNQDLKNTTLQKCTQIDQFHVPFILNKTKNSFTWWQTYNMVTYVYQESDFLVKVFTSSGSSQETGKIEFDSKDLTNKINSFVYLQNAVFLVQSAKYQISVWSVRGDIKELYVINQNVLRQQGYNGTFIPKAVFGNPKIRGQIVFIQTQEEILIGEFANGNNIHVFHLMHIIPIIQKSQVSIAIGPLTFTIVQKTASEQIIEEYNWEHLQLIALNKRLPLYKYQLQEPLNIDYCSQSGWLFVRAIDTALQETVILVYEPNVLSHVSLHKVIQTGYKINDVFDMAVDGNSQMFIYFNDNKVNKFITLLQDAVLTIEPKEDILQYVNKLETSVQISSALGGTPLVVNEKIKLMNTDSKIIVKQSSLDKYKYMFQFKKNDNDQSLEIKSDWYIGQPYDLFVLCEQCGDQVLIEPQIVKSSNGSDMKFVIYDGTQFDSDTLVYQADKSLIFQHKNGSFLNRINLEASLGEGQRCELLTVTKDSKHIISTVIGIGDIYVLVNECTGTKCQQYQQGEVTLAGVTQAIKMRFVFGRNFIVLNTDPVNYSKFESSIIVYSFNTSNNQFTILSQKVINTKYLGATSQVQIADFCHLTVSEFIYAILFTDITKGVWIVNLGSNVDGSVTELNKELLNLRGYEHDQYYLQDDTHFHQLKVLSQEQSDKIFKIQLLVTTSNVANYIFYFELEISSKTGVSYIKQSLKLQQILFNYGDWKSANKMSFAANHVSVAYTDGNEVVLAVYYIEVKKLDEVRTVPFIYGIRADYNQPLPADFAMIMSQYQNQTYLYANIEYDSQFSEHTVYRYTINDKPKLILKNCSTLKEQDLEIYAVNHFGAASGKTRLWDSDNVPDDDDSSSSNWWWITLIVIFGVAILGAGGFFLYKKFGKKGVEPLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PPR5</accession>
    <name>A0A8S1PPR5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Protein kinase domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T1270036</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PPR5"/>
    <dbReference type="OMA" id="MLTEYEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000013">
        <property type="entry name" value="DnaJ subfamily B member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000002">
        <property type="entry name" value="Molecular chaperone DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="137"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="17">
        <location>
            <begin position="394"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="15">
        <location>
            <begin position="484"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="16">
        <location>
            <begin position="598"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="11">
        <location>
            <begin position="598"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8105367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="818" mass="93758" checksum="207F0730BC57373B" modified="2022-10-12" version="1">MKQAPKLSEVNSPNNKLINILLQKQKSPSQYSIQSPPVSPTQAHLPKGQYQSKNTSIDLSKLATLMKQQKVKSPLDGQKDSMTQLIEKARAIVISAKTPTGPIHIRNDSAKVRKENKVPEVVSPKVSDTKQIGKALFTFEYVIGIGGFGKVWKVKRIGQLYAMKEMSKALVITKKSVNSVMNERILLSQLKHTFLINMNYAFQDRENLYLVMDYMCGGDLRFHIGRMRRFNEEQTKFFVASIFIGLEYLHSNNIIHRDIKPENLVLDEKGFVHITDLGIARVMKPENSSDTSGTPGYMAPEVMCRQNHTYAVDYYALGVIAYEFMLGRRPYVGRSRQEIRDHILSRQVQIKSSEVPPNWSVEAVDFVNRLIQRKPSNRLGNNGGYEIKLHPWFKNFPWSKLQNREIIAPFIPNPSDDNFDQRQIVIEDEENAEIISQNIQLLRDPNVQQQFVDYEYQMHNNEKAFLSLTLFTLLISIYCAVHREYYDILGVSPNASVQDIKKAYRKLSQQYHPDRNQGDPDANEKFSKINVAYEVLSDPEQRKKYDKGGVDGLNNQGMQHHDPFDIFGSFFGREQQGERKGPELKVKVRVTLEDIYNGKEIPIYLTKQILCPHCRGSGADDPDLVETCPACKGVGSVQKRQQVGFGFFQTFQATCERCYGTGKIIKKKCHLCKGDKIIPGADNISLYIEKGIQDKQTIKYENMADERNDSGTSDLIFQIEQIPHTFFQRQGNDLKCKVEITLKEALLGFKKKIKHLDNHFVRIDKEGITKPGEVQIIKGEGMPQHEFSSQHGDLYVEYKVVIPNFNGEQLRQWQKFFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRN0</accession>
    <name>A0A8S1PRN0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1280051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRN0"/>
    <dbReference type="OMA" id="PEQNSHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="27365" checksum="5B0D2053CF9AA15E" modified="2022-10-12" version="1">MQIQNKTESNISSEDDILEIIQTIINIKQQQNQMNFQDNSEDQSNLQKQTEQILIPQRFRFKTSHNLGEIKQRKLSQYTVKSEIDEIKENQSNDNEYLQDLIQISPKKPWHFKSVIIDSSKNSEKMMEYLSEQQIKSQNQKSLASISSNASFQDLGIVTLKEELQTVYSYDQIQEDKKEALIYCFDTKEKNQTNPSISNNTKNSYYDKNRSNFQIISLILITIGVAGWVYQRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRX7</accession>
    <name>A0A8S1PRX7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1280091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRX7"/>
    <dbReference type="OMA" id="SIQCNDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="147" mass="17125" checksum="45C967A4CE4D5379" modified="2022-10-12" version="1">MLQQFLKGKNFAFEIPIPISVADEYESMKPTNSIPKIQQLKQTQTATLIKLQSLKLHPLSNKQSTITLNDFQKKLPLEEMIKLRLEMKFQTPTSFTETLRSRKFASNLEQIRLTHERDGIALSVEHQKRGLVRYAQKLLNQQQITFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PS02</accession>
    <name>A0A8S1PS02_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1280106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PS02"/>
    <dbReference type="OMA" id="SIFYFEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="47032" checksum="D2AEE5FAB447245E" modified="2022-10-12" version="1">MQVDNNVEEEKKKKENEEESKGVVTIKRRLIASFDLFQFMPVPKTEMVSSRRSLYGSLLMIGCFLAYFIYTLVAFITNNSPTVNQYEEILEAQNVTLPNIAFGYFFGDPFTKTINDPSIFYFEMFQVTKFQDVSKKDVKTQIPLKSCNPDWVPASNFTILCPNESANMSGALYQTPEFTYPRIQVTYCSQGGNQTCISQQEIAQIAAGGRILLYIQDNSPNFDLTTGQSVESVPYSTYQYFLMPGLYPRTEIFFQLEKYTIKPDFLRRWQDDVRIFLSTEKINSWITNVSVYNTYNAYQIGFRTELTQQISVLTYQTSFDMISIVGAFWGVLFSTFAAYFLVYNQNTFYRENKQWEDFGKKIGVRKAADVFNLTEEAPHSKVNDSIVFEMTEIHQENNHSQNEQNGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUR7</accession>
    <name>A0A8S1PUR7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1320125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8107057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PUR7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="38007" checksum="E756E6C36FA6F89A" modified="2022-10-12" version="1">MTSNLHNQLETLKVQTLNYKLYTNIQILESWCCSISMNQIKTLLIAGCSYKTLIFQFCDNPKLLVSLIEETYIVSSISQKVQSSEVLIGNSKGTIKIFQKNGINSCRYLIKSKEHKGLITQFILTKDEQLLISSSIDGKIKFWNSTQLINCIQTIDCNLDGVLGISLNQSENQLISCGKQSKIVVIQKSNINQLWFISQEIQVQSYGYRLSFIQDYAFTYQPFYIDKLEIYQSNQDNLLYNKINEVNVKKCYMDCLFPQIFIKNKSLLINKMGNLIYIFQIKDLTEIVQVQIIDFNTYNIHGSVSDDGDFLFTWDDNTDLITIRKLKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXQ1</accession>
    <name>A0A8S1PXQ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">60S ribosomal export protein NMD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1350138</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as an adapter for the XPO1/CRM1-mediated export of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NMD3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8108030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXQ1"/>
    <dbReference type="OMA" id="QYTHTEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048899">
        <property type="entry name" value="NMD_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048898">
        <property type="entry name" value="OB_NMD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21193">
        <property type="entry name" value="NMD_SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21192">
        <property type="entry name" value="OB_NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="9">
        <location>
            <begin position="10"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 SH3" evidence="11">
        <location>
            <begin position="233"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 OB-fold" evidence="10">
        <location>
            <begin position="301"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="391"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8108030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="445" mass="52939" checksum="350236BF786384D4" modified="2022-10-12" version="1">MLQRAVLMFCPLCELEFEPKLTKTNICPNCLIAQMNITQDIDNDKEILFCRYCRRYQRPPWVLCERDSKELLAICLKKVIGLQEKTIIDTKFIFTEASTKQIKLQITVQKEAMNGTCIQDSKILNFEEVYHQCEDCKREFTPHIWGACIQLRQRVNHKKSFYYLEQLILQHHMNQQMLKVESADDGMNFYYKQKNQALHMLEFIRSYLPIQVKESKEVFSFIKYTFVVDVPRICKDDLVILPHKLCHQLGGINRIQICYRVASSIQLIDPIKLKVCEISAEQYFKHDLDIRTMPLRENCDEFTVLDVEKIKPQKNDQNNPFQNSFCTVELMKDNDSDYKQYQVKSHLGNFLQAGMTVQGFDLNKYNNELDEIYDKPELIIIRRKPEKNKKRKTQLKRLDIEENQEKRNKKKEGLEEQQIEEFLEDIEQDQHLQETLNLQQIQSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZ72</accession>
    <name>A0A8S1PZ72_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">UV excision repair protein RAD23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1380008</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZ72"/>
    <dbReference type="OMA" id="PHMLEPI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14280">
        <property type="entry name" value="UBA1_Rad23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14281">
        <property type="entry name" value="UBA2_Rad23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01805">
        <property type="entry name" value="Ubl_Rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000002">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000003">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015360">
        <property type="entry name" value="XPC-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621:SF0">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09280">
        <property type="entry name" value="XPC-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="132"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="267"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="78"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="317"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="91"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="340"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8108687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="38405" checksum="95C2E294EFB8072C" modified="2022-10-12" version="1">MKIIIKTLSGQNFPLDVEGTDTISVVKEKIFQVKQFEVGQQKLLRKGTLLDDKTTIAELGIQENEFLVVMVNAKKTAPQQPQPVQQPPVQQPAQQQPAQQPAQQVPPQQQPQPQGAPVVKPAASGTGLLSGPEYDKAIEELMSMGFQREECVNAMKAAFNNPDRAVEYLLNGIPPGAISNPPAPVVGGQGQPPAAQQPVGQPAQYQQLRQLYQQNPQAVMQLIPQLMQQLQQTNPELHAQVAQNPEMLLQILMGGGGQQGPPPGSIQLSQQEFKDIETIMGLGFTKQDSLEGYLACDKNVEMAINYLFEKQANGDLFTQHVQREQANPPNQQPPGGNDGPMDEEDDDDVYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q907</accession>
    <name>A0A8S1Q907_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1470009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q907"/>
    <dbReference type="OMA" id="ITYSFAI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="254" mass="29232" checksum="82177D4F1DACCB49" modified="2022-10-12" version="1">MLIKIILALVHISFAVIFDISEYKMPLYVLSAILTMIGIYNSNKEFGFLLYTFITFINTFIKGFILEMYLHNRHSLLDPKSLKLASYVFIITIVLGNPFVIRRISSFLDSHYTLKFGLNLSDKISQCITLSNCYHHVLSQANPSMFELGLITTVGFSITNLVIYFGLLESKSQGESIKIMRQSFIDNFITYSFAITNVYLWNFHGQQFLNVKSNNVNDIRIVVSVSFIVIYTLLSMLHSKHSTHSIHKEKEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9H3</accession>
    <name>A0A8S1Q9H3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1480076</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9H3"/>
    <dbReference type="OMA" id="QILFIYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="361"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8111634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="427" mass="50437" checksum="D6B7C209EE25EBAC" modified="2022-10-12" version="1">MLIQKTKIKKIISYFCIHYIYYNLFALLFALFQYDRIPFAIPLGLSIALEIFICYYYSNKEIIKMVDHVLHIMAYNFVIILYYTDLQYYIFGNMFLLLSFVIRTKESCTNQITHEYSSIIRFLKTFYRFSSLVASVCISLKLSKYVEWTWPQAFWWYWMFLSALVGTCFTLIIVLISKIINEIFKQYQTVPQEYKSLLWALYISIISSMLSVMWIINTFNKLGLDLPIKIGEVLFYSTISFNFIIFGAITKLLWDSIIDFCVYLLQSQQTEIESQRELPTCQVSQQKQYERKKTIQQIFLKKLSSAFFRQTTKSDLIQQSQKMTELMTERGQTKKILQTVEIESNQLQENQNKIIESNQKCIICCDNPPNAVLMICGHGGICYKCGLEMAQKSKECFLCRQQILFIYEISNFNDDLMKVVTITKFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QB51</accession>
    <name>A0A8S1QB51_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1480049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QB51"/>
    <dbReference type="OMA" id="LTPHYPL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="573"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="674"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="764"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="252"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="587"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="601"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="772"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8111580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="940" mass="109908" checksum="9742982FB11F5FE0" modified="2022-10-12" version="1">MEEQIKRLQSFVEHLQKKSKINNSKLDVECYEVFSECVLIVQSQISVEIGNVMDKLFNLIREMLEQAQSQIKPHPEPKSILLTPQLPDFINELQLGSVNIIDQQGTPQYIKNYQQIPWSDYKCRGQGTRNLRTYQDVGTSTIIDYYQNCQKASSDILRREEEMREWLEFTNDTISMLSEALANKKQLDIEFQGKPEELDIVISDIQFKRLNLEQLMKSVNQDNIGIQNYDNTVQFLLQHIHHIQQQTAIAIINRYKQRYQEKEKQQQMINDMKSKIKKKVNNNNKEDCRCSQYVNEILILKNKLESAQNDISKKDTQLLELQVFNEQKDKQFKIMQAQLSQLEGEKSEFKQKLDNIYKEYSKLIKKNSDRERGYLSSSSNDEIIINAISGNPRTTSRDQSQQQSSKQQIYLSPKDNFNIQKKTQQMERLVKLVNALDGNNQTRRLTQMQFILEQKASSQIHELADLIKDIEENDLLQNDNMDNSQISNNIDQLSSMRNLEIKFKQNILSKNLLQTSFEIESNNDKPAIKVSLPTEQSQHQQIINNKNNSTTNSNYKRIEQQGNKMDGLKIINTSPNQLTPHYPLSKAKSSQNINRQNSIKLDQKDKKETKDQYQQTDLTMISNIFQTDKQQNLLQEIEQNEQNENLKFQNSNNKINSQQQLTTSSTFNFNSAGTMGIGNLNSPMQSSNTNSNLPSPRNQQSSHQTNIFPSPSSSIRQKKMVALKLEQELYTSQTQNSLQQDQKNISKGKSLKQKIYEQQEEKHLNQQHRRMYTTTGTQSQTISPREYYTPRNKTKDDLVLNKVQQEQDDKQKEEQLFYDQMTNEMPEQNEQDIIQKAINRIPGYELAQEINDKILETMFGNLNKTPDIAQMIIQLSKNGQLNLNEFKRFINRMQTFHKRCGRDCNHLMRFYLRLGFVSMKYLNKRRLLKLSKPTVLPGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBQ6</accession>
    <name>A0A8S1QBQ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1530043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBQ6"/>
    <dbReference type="OMA" id="TRFNSEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="284" mass="33854" checksum="D0304164E283E2C0" modified="2022-10-12" version="1">MQIQKYHLDKYISMILNKRAYLNQGTSNSNILNYTQNSKTNDQNIAISIYLEHVTDHRKILAEQWILCLKNYQVTNQQCGKFCQLENFSLCCLLTKSPLYQNFIVKQSSLKNYQLQIIIDHNGKNQNLELYKCYHLNMQQNLGIIARFHQHLNLTQEINKQRSLSFQEQTVIPKQNNKLTRFNSEQIQTPEKKVFMYFFEEDVTFLIDETQVERVMSQNSDSDFMIQTIDDETDVTEWQVIDHKQLLSKNQKKMLDDLKQIRDHFNFKSKHSHQIQMTIGFIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2024-11-27" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QEU7</accession>
    <name>A0A8S1QEU7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">mRNA cap 0 methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1560100</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004482">
        <property type="term" value="F:mRNA 5'-cap (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004971">
        <property type="entry name" value="mRNA_G-N7_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039753">
        <property type="entry name" value="RG7MT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12189:SF2">
        <property type="entry name" value="MRNA CAP GUANINE-N7 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12189">
        <property type="entry name" value="MRNA GUANINE-7- METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03291">
        <property type="entry name" value="mRNA_G-N7_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51562">
        <property type="entry name" value="RNA_CAP0_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MRNA cap 0 methyltransferase" evidence="6">
        <location>
            <begin position="1250"/>
            <end position="1463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8113774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1490" mass="174620" checksum="873FF59822E395FB" modified="2022-10-12" version="1">MFKQFYLELLIIIALKISNCYYLSELLQQYPFQIISFDRNQMKMDDASCSGYGLWSRYIPLSNTVKIGQIGILDSNCFHLHNAQDQNTQRINLILYECLNYGTNTIKKYLQFIDNNGVDHIYEMPIQDGAYEFVWYFIGFEMVPIENRFTIYFSNHEKLIQTHQIQIEFPFYDEKLNLIFGGDLSVKHDQQLHNSENEKLSYFPGQVLILNYYQISQQQYCNTVISFILSSQIDGCRCVPSQNTKINDQVIQKQEEFLFISSKTNCQQFLLSGWIKIDDIHTFVDEFDYQFIKLSGNFQVTQMTDDNLSPFTLTYKISSKGNQMIITTYSYTFPSINIDFSNNPYLIKQAFHLIGDIKLWHYLFVKKQETNIYISITFFELYKQEKFEIYEEVKQFNLVQFKLLYGNILQSHSDYFKIQIVGFQLLNCPQESVEQLECHSTCQECDGPTKTDCLSCFEFSNRKYLPDFKECICEYGTVDMNNQCITYESLNLTIVTPDEVTMQSCKYGYFEFDNACFRCPSTIMDNVVTCLECLQNPKEWMNMLYCETSLYSDDDGNISQYIYETDLCYKFVGNDLNYYLCEDECLDCQFFEEFNSNIQQKQLSSDKSMFIIAQITYCLPPYFLNFEKICTLCNIEFCLYCFSYFGNDQTKTTLNQQFQYSIIGEEIKTGCIQCIDGFIYSFEKEQCIYKNPAISNCLRSYLAAEGQEICIVSSIDDFNIAPEIINCQKHILKCKQCIQTPELIIKCIICEDGYMASAYSGICTECSYQINSKICVEWNQKNLEPWKWLIQGFRIQFLKITNFYHELVSIVPMSYVIQCLDGYQIILNQCYQYCDDNCQVCRNMKIDNQQQFYCQKCGLNYFKKNNRGQFQGKCINCPSLCQMCEQRSNDQIEKINPFFQINEENSIYTLKCIQKVPLQKVTIDPNLQIAHYCFNENCDDMFEYNVHFFCRTLELLDLDSYSGYLEQKLNYKYFNEIGLKRITLILLFNTVCENFQVKNYSFLNYIREKNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQKEDKKKQIQKIYLMETKADSDSTLFRSRLPQDLYFDFVSMQFMANLLFSSEQAVENMFENITCRLTNQGIVLMTITDANVLVKKMREFTVKDNEGNYVYSKNQYFSLKFKNLQFPKNKPFGQQYYFYLEDSVGIKEDNQIKYVPEYLIELQSFEQKAKEYNLEIIENLNFIDFFEKYKQKHSNLLKIMVKPPSDDWKMPLDQWEIAHLYRVVVLRHLKGQAQSKIRRHPHLTELPDTVLELENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFJ2</accession>
    <name>A0A8S1QFJ2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1610028</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFJ2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="247" mass="29072" checksum="FB65ECB96A78B6E0" modified="2022-10-12" version="1">MLKNPKIKETLTSRLQQQECCKLQGHENNLIQFVCACHECPYRYQKACAHCVIKLHGKHVEDMKEIEEFDIIINENSKKAQSLIKESEELFLQLKNPFRNFFEQLKLQLNDMIDSVQQRYLIQNQERAQQLIRNELDFINQFNQNVNSKQNRYLNDHSLKQYSNSLRTAQEKLSNVFSLFELKQLLESNLIVLIEDFGKNNGISYISNTSRNISQRHSSCHNIMTPRSALNVFNHNISVARSKKIRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFU6</accession>
    <name>A0A8S1QFU6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">OPA3-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1610115</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OPA3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFU6"/>
    <dbReference type="OMA" id="QEMFFKP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010754">
        <property type="entry name" value="OPA3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499:SF0">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07047">
        <property type="entry name" value="OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8114849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="20702" checksum="AEBFB8C7BE9C4F6A" modified="2022-10-12" version="1">MIPIQKLASLLIRTFSKPLSNQIKRYALNKHRSRKPSLVKTAFVFLGNKYHNVETFINRKSIGIKSQEMFFKPLTDEAALNKGSDFFADIFVYACILGIPLLELYKSQRDSAKKSQEQDTALQNYQTQIDNLKQLNDQFQIEYNRIVAENQYIEQSLKHFEKDIDDQVQHLQKQLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QIE4</accession>
    <name>A0A8S1QIE4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1600024</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QIE4"/>
    <dbReference type="OMA" id="NESYLIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="117"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="472"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="775"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="353"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="366"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8114467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="907" mass="105857" checksum="E768F491869B7708" modified="2022-10-12" version="1">MLKCQTLYKTILKYLKTFQNQQYQQWKWHNYTQWADLSFRPLLIMQIIFIIIKLSIVANNLILWIILGLQILVHLIIFLCLPGIIYKESYLLQLVPLIQQECLSILCYFIINESYLIFYTAICVIFQSMYSTILQKVIAIIIYLIQTMYFADLQSYEGLYLTITLLFMQIIRKELQQMQHFLNLQALFLIIDSTPQAMCIAHKDKNWLLYSNKVFDNLANKLESTNQSETDQSPEKSGAINFTKNQLTFLQNLQLDELNNNQNAVQFDFDDDDLYLDDQYKIKNVTYSKDKNSIQYSKALSLEIPTDRPKNHVQFQKFFQSFKNPQDESIIQKTFTSDVTIRIEVNNPLQFPLQSAGKKSQRKKSNLPNKTQKQPNYMVSSRKISADFLLNSKEYKNSGHKQSLLDQEVNFLNKTKPVYVQKMKFLINVLENCRIFDNDDELQIYFIHEVNQVLLKTKLKKLESIKKNLLRSISHELLTNLNAVYGFIKQSQDKLLKQESCHIQLEQALSYTKLQQYKIYDFFDYRDILEDCFQLKQDKFEINTVILECVDLFRNQIERKSLIINVELPKTSYIISGDRQRLCQVLLNLIGNSIRFTYKGGITIIVQKQEYIESMEGIDLNSANFQDNHFLEVQINDTGIGMTENELSILRKKLHLTDEDEKVSKHSVGIGIGLSVSKQIIKLMSPKSQNYLSVESTKDEGTQFNFLLKYNEESNQNSNFLQKSNNQTIPNVDAQSTQQVRVMNYNKCNNIQITEKQGQSEIQLDIVLNECNCKIILIVDDEEFNIQVLSHMVKQLGFEIDFAFNGKQAIEKVQNQLDKRCNPSYCIGYNCILMDINMPIMNGWETVQRIRQIENEIHLTRTIPVIAVTGFCSIKDQQKSINEGFNSVLIKPATKELLIDTFNRLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QIZ4</accession>
    <name>A0A8S1QIZ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1630019</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000170">
        <property type="protein sequence ID" value="CAD8115184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QIZ4"/>
    <dbReference type="OMA" id="DYIIMIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002483">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF139">
        <property type="entry name" value="PROTEIN SCARLET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="358"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="471"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="567"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="33"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8115184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="598" mass="68111" checksum="0534C3EBA3200149" modified="2022-10-12" version="1">MTETIKQEMTPLNTNKPEPSTSKTLRSRKVVDLVFQNITYEIKKRVILDNINGICPGGHVTAIMGSSGAGKTTLLNVLACRITNSKTNKLTGKLLANNCPYDYEKFSQFASYVMQNDVLMETMTPKEALEFAANLKYSDTQIKQSRVIETLKCMRLERCQNTFIGGISMKGISGGERKRTSIGYELVSNPSCILLDEPTSGLDSFTAFQIIHQLRKLADEQDRTIIFTIHQPSSDIFLLFDRIMLLVQGRLVYQGSRENIINHFSSFGFKCPDHSNPMDYFLSITHSEDKQNIENYSLYFENYQQKLQKQIESEILNCCNSDVPLKINETSFTYQIGEISSRGVKNIMRDHMQFRAKVIQAIFLGLLKGGVFWGAGRNNGKLEDLLSISGALFFICVDFTMNAIMSCILSFSVEREVFLREENSKLYTTKSYFLAKQLIEIPFCVISPLIQQIIAYWMIGLNDQSGEVVLIHLLISVLVFVCSNSMGLMAGAAFKDIQLALNIVPAIVTPLIIFSGFYSNQKTFYPWIGWLQYLSPMKYGFEAMSYNENEGKYYENDPEELYGFDIGLWWCALILFGYIVVYRFLAFLFLYGLKQKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QK57</accession>
    <name>A0A8S1QK57_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1620092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8115067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QK57"/>
    <dbReference type="OMA" id="QVSHMCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF9">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8115067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="317" mass="37082" checksum="B446C738BE3F4A88" modified="2022-10-12" version="1">MNQSSWFSIEDLELEHQTSEVNPPISKERKLPEKLNMDSILSQYKQIEINSNLIKYIVSESQTTDNNKQINVKSKIKLKCEERDLEGNLANKGTNNIQATKISMASSIPYIQGLRSAFLTMKEGDVAWFKLSNEQMYTEQEIASGIQVQNLQKYFKIDILEVTQPELPIDITSLENRLKQLELFKIEGNQFYQKQQYQSALLRYQRGLNFLEKWPKKFEDNPVAIEAKRNSLLVLSSNKAQCLIKLQDFKQAIQVLEPLIGQMRNKEYEVKNYYRLITCLKEIDEQVKADFYFGQVSHMCQLSQDQKRLFKSIKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKL1</accession>
    <name>A0A8S1QKL1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1630032</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000170">
        <property type="protein sequence ID" value="CAD8115210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKL1"/>
    <dbReference type="OMA" id="IWECADN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="11">
        <location>
            <begin position="23"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8115210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="263" mass="29904" checksum="16CF3F0354CD84D6" modified="2022-10-12" version="1">MGIKGSKANLSQKTTRAHSNGITRSSCQMQGWRDYMEDYILIQEQEEFQLYGVFDGHGGQDVSEFLSEHFYEIFEMELKKNPENYNLVLESTFETLDTILAHRIASSKVGSTAIIVLVTKEKVHIANLGDSRAILFSNESVQQLSQDHNIESEYNRIISNGGYIRDDRINGSLTVARAFGDFFLKSSRCSIISSKPDIMMIDRPQNKSILLASDGIWECADNQYISKYLMENNSLGKLFNQLIAKNMNQEYGYDNMSAILIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QLH8</accession>
    <name>A0A8S1QLH8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1640007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000171">
        <property type="protein sequence ID" value="CAD8115355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QLH8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8115355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="102" mass="11861" checksum="A6B5B202EDC9DFFB" modified="2022-10-12" version="1">MSLDEECDDSNVLNGNDRTCRLEQQFICKDSVCIKPELLIQILIQAGDQSIQYNILLQFNNDIDFNKDVEVFILNGQEEINIKQQCDITFNMNNQFIQTQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QUZ5</accession>
    <name>A0A8S1QUZ5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T3200001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000329">
        <property type="protein sequence ID" value="CAD8119419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QUZ5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8119419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="47297" checksum="6CC6E1F59D075438" modified="2022-10-12" version="1">MTRKNEDNMSMLRLGRFANQVSIKTNKLLFYIWRYSGILLKRVIVYYQFILLQQQSSPMTNKYYFCKTFSQFMSKIQYDPSDIGFQVILGNMIIIFLLDILCLCAKSSMTPQIQIVTSPVEIQVKIFYNFLFGQYTSHYIFKEGIQKSSREIQLCFFYNRNRSQLYYQELIISIAYILFAIIYIQMKNGKFQIVITNYFQKEYGNIIVVINVQYLLICIIRVLRLIIITQHLLTTSPYEYQLDSGFCDNKKYEILQINITQNKASFLNFTISTNLVGGFILQIHSGWWHYIINPAFFLYSLKSIKFSKTENLDVLSPLTATKSSLVHQSAIMLYLMLPGMQNLIFNTYFLVETTPQVYLSKTSRFSLKVDLVNVIKKFEIDQQEGKISQVIKKEGSVFLLLMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QVH0</accession>
    <name>A0A8S1QVH0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2560003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000265">
        <property type="protein sequence ID" value="CAD8118697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QVH0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="72" mass="8566" checksum="212652627ADC3CE9" modified="2022-10-12" version="1">MSDNLFFQVTLENKTIFICNPYSSRHMSLFSKLEQDSIILSTTTNKQSSFYHLWSKSYLKEMNHLFIKITTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1R007</accession>
    <name>A0A8S1R007_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T4000002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000409">
        <property type="protein sequence ID" value="CAD8120000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1R007"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8120000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="39114" checksum="FF8A4632803CFCC7" modified="2022-10-12" version="1">MKIIQLRFKNYWVNFLMTMEHEEQKLKEFNIYISGIYILFCLRNIDSRIKKLCHSFHLFTNVILDARVSYNCLGNTYERIMLQCEQRICFEMLYRSSVPILKFQRIYWIILFTQKSVRSIISICQGKIRVFYIPFIYQSSNDNPANFKNFQILQQPLLSKGTYLFPTAFLAIAYYSTKTTNTFQMICWLFVQIETTVDRTNAGKCFLMNRSTSAETQLLLANSLNYRILEDIKSKAVLIIYYLIFCLSLQSIILLQNSQRSQKSYYFCNILRSKLSNFDSISLFFHPLISKQTQYGIVLYKKAIIIIIQPVVTIQMQFLLYFWIYLIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPT9</accession>
    <name>A0A8S1JPT9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0060203</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPT9"/>
    <dbReference type="OMA" id="DQYNSTQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035253">
        <property type="term" value="C:ciliary rootlet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097542">
        <property type="term" value="C:ciliary tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033192">
        <property type="entry name" value="ODAD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46518">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46518:SF1">
        <property type="entry name" value="OUTER DYNEIN ARM-DOCKING COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="466"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="323"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="470"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="487"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="525" mass="62399" checksum="4F0E3FC55BCFBAE9" modified="2022-10-12" version="1">MSAVELPDIRLYSNKSNTRQSKKSDYEKYRKLAVIKSVDKLIKKNDLQSVLGFDDDYYPKQIEDQKKVNEAHEVHINLTELKKSCDSMKINGIKKEKDIENMRKEIDSMTIQVDQYNQTQNNLQVKEKELQQKLENQHKLLSESQYDRKIFEHMLNRMKKDQLVYQLRANQYERHLQLALQQHHSSTYKTQQIQEIYSKTLMALKEVTEGIQQQNKNREKNLQRFKDDLKQRQDIEQKRDDRIKRQQEIAEVAAKDIKDSALKKWRKLLLVHKFLNSFLKNKMQRGIAQYQDLEMTFQKIKASTGITDANDIVQKFLGREQTYAHLLISISDYEKKINALKNENQELSTQFSSLKQQYTDMDKEFQVETYRQKEDQFEQDKLVQEVEEKATVAGLLENKLRNWMSRGLKKLGLQKNLGFQAIIDTIREKLLNLSVKQQEDLLNQSILNAVSDIKDQEFLKRNVRVKPKKQLTQSHTNHSMDQSSIKDDDDMFDELPIKEDEEDELEEEKLIEQLREEIKVKNHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JS97</accession>
    <name>A0A8S1JS97_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0090466</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JS97"/>
    <dbReference type="OMA" id="LESEERX"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="687"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="98"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="433"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="687"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="698"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="726"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8045532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="767" mass="91993" checksum="84DEF9B6E0C8AEC1" modified="2022-10-12" version="1">MLPKNTISDVGTQVLKQYKKMYEEICYCLKQEKEASIKELEIWKDKYDNLQKRCDILNQSKDKQDSKIKQDLNDPKTLNQVPKISGHQQINLQPNAEKQQFQILIQQLNDQIIKQKKQIQDLNDKNEKHLIDNKKLEIENKKYSDQLNQIRKSSHHKINDQQMLQYEDNIQKFIQTIDEMKKDKNNYEKQLEKLTKENQILIQTNDELRKVINDHDKVQQQKNQFQLQLSEFGLKNSQLEKLVKDLEKQQQHQQELHQQTTKQIEDKQQEIELLQNKIQEYQKELSNLNQLQEIYDEQQQQLQKLAQIIQELEGKLQESTNLKQNESGKKQLSQKYDELYQQYSDLQTQDIMKQEWLNLQKSQLEDLKQENEQLNHKIDEVQKQNNILISQLEMNKDHTKLSFSQLQDQVNSYEHIVSDKNKEIYQLKLELAKQKCNLQNDDYEEKIKQLNTQYEKLESESKMKIEWMEIQNSELEETINEYEKKIQNFVEQLSQQSKNNPKVDNSEKIAELQEHINQYELIVNNKNIEIDELNKQIKEINQKQNDEDFQKKYLNLITQYEKLQTENQMKQQWQSILEEEQQDQITQLNEQLAQIQNINQPEQIQQLKDQLLQMNEMKTIYEQQLLDQQTLISNLQQQLEQISDQEYYQKMNQILQENEELKQLRSDNQEELNKKDQMIEELQMQMLSQSQVTGSSDLQERKRRQELLDKVKDDKSENSASSNEQSIKKIDKQTSETNEKQSPKLDQTQDISKSSKPKSAKSLDFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSG8</accession>
    <name>A0A8S1JSG8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8044985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSG8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="434" mass="52351" checksum="191F09BF321C601D" modified="2022-10-12" version="1">MNIQVHFYFKSFKVIDLLEDSLTYYSKRISLIYNIDKVSKLFLIQNQQLNQRKIKNQLYKFYFQRNIIKKIQNSRLIVMIHKLKNKFLMKLKLIQKAPKQWIFRQSTCSQTDKILQKIDFLISQINGYSQDIINFSLKNQILCSKTAFICEVLNEKICKNQLRTQLRKSSSLNHNQNMYIKEMLQISQTIKKSVQVEKQDQLFGMKSLMQQLPLVRRIKMDSHEEEDTQKINYQDLLNISNNKQNINDSEELILSKKCLKMEEREVSNVLQSQKYFKRMSKCYQIQNQLTACKQIVVSSQKHILKINQNQKIYIGQQWFPFYLKLFCQDSKSSWQLFIKAINYLQKKGVNYLLKKERAFQIMKNFLTMQKLEIQNQIIFQTNNQLRSYYFIDILITPQIYSFIQLNSTFKYTQINQFSTFYYNLLNNYFRFQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSY2</accession>
    <name>A0A8S1JSY2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN motif protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSY2"/>
    <dbReference type="OMA" id="DRYYIGF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="54955" checksum="642C9DE73AE94F45" modified="2022-10-12" version="1">MQQQSISDFDNLEREHQLIDQVNQKSKSAKFKQYKQGQYYGDIQNNIRHGYGLMTYQDRYYIGFWKDDKKHGFGKEVLDKGDQYEGQFEDGKPHGEGTLETSNGIYSGQWVQGIKQGYGKWRGKNNEIYTGEWKFNKANGYGRYDYQDGGWYEGEFKNYLKYGKGKENYANGDFYDGDFINDKPDGFGVYRWADGSSYHGTFCSGVRHGKGYWLKNGDPKNHESYDGEYVNDLKSGQGVYRWPSGNVYEGEFFNDHRHGFGEMRWVDGSFYKGQFHNGQMCGEGELKRNNKPIIYGYFENNKLVKEQKKKSIPKTMNQSQNNSYDGKGNYYSLYQSFNQFQNAQHLQKYHFKSESIEPIIRQFKRDCNTSFDEINKNEISTHQNRFKISIDLDEPQGMTTQQKYKSLNTTNRAFQNQQENLISVTNRTQATKKVIIDSVKFKRSQLSFRLQSLNQSTRQTQNSLPKQGVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSY7</accession>
    <name>A0A8S1JSY7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0050394</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSY7"/>
    <dbReference type="OMA" id="QGYLEYH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF1">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="4"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="460"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="631" mass="74857" checksum="55175B0235C20485" modified="2022-10-12" version="1">MNQRPIKNGYLQQKHIFGLKTTKFYYLEGTQFLIFEDDKTHIPQERIDLSGFIVDGTWQEDGYYTFTLRHLQQEIIMQFITMTYDDAAEWVSKIKQAILISEYEALLHQTSYQISIDQNRSYKNDSSNVTKNIPEYVQKQLQLYQELSKDKWIIEKTLKQMKLTAIQSQNNIILKGEYVFNTSLQNVITIIQKGGKLLDLFRKQSDVHEIDLNQFHQDIWHFNNDGKKFVLESKYIQFDFQRNNSFFLTKESITENQFPMIKTDEKQSKKNMISIFKVLEIIHVVEEDSKCFTQYMYVVKKDENEKIIRKLMKEQIINLSIISTELDLLLMQINNESIPIIQSRIVVGTDHSNQENQENEFIQGENIHFPPQDRLGYVDHQLQPNQPAAIHENMHKRIIHQNEEQLKALAELKEKIGHLFLNDQTMIRYLIARNYKVKETEKMILKCLQWRKENKINSRKTSDYQIYANENVHTQLGFSKWGHPILVTNGMNSHPEKFENEQGFSEQGYLEYHQSLMEEGIRSMRGYVDQFIVIIDCYKLTPANFSFSVLKNAFIEIFNYYPERQFRIYVLNTNFLTRSFYAMLKPFLPSRTVEKINFIGQDFNEIKNALSRDLDEETIPKRYGGKNILIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWV8</accession>
    <name>A0A8S1JWV8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Polyadenylate-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0090135</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWV8"/>
    <dbReference type="OMA" id="HHTHTHC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001548">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:002151">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001005">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="51"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="134"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="227"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="5">
        <location>
            <begin position="532"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="438"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8045110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="74146" checksum="CB25DCA2DA17FF90" modified="2022-10-12" version="1">MKYIKHRIKFYVFKRQTKAQKIKRIFKSLLLNPELMFRLPNYQDQNRPSENGLHIADLEDGITEEQLYVEFRKFGQITFVKLHRFPFIGKSKHFAFIYFSTQEEARKAKEAMNYKQLLREPMRITYIQEYDKDANLFFAGFELNVTLQQLEQFFSKWGQVVSVKLSVDQNKKSRGYGWVQYERKEQANNLLTESQQNEGKVQYNEKTQIIVKRFVKKGTNEREDKRSNLYIKNFWTSLDTFDLENNQVREQLEGEMRTKLNEWFSTYGSIISILVKIDIERKAPFAFVSFSRHQDAKEAQKTLGTTLGTNAQKDPLNTGRKMYVGWAQTKTDRKQLRDNNFYSKYIYADHLNRNVTEDMIRQTLKDAGYGDICMIRLEKMQQGFQQIIRIGYIVFDQASDANKLIKSFKENEKFDEIKKLFDQNVEQAGGKYFQHLFPQLSQQRGQRRPNQRQSPTRRMYQMPPGPPQYGTRMPFPFPMQQRVPGGGMRRPYQQQFPNPMRQAPLPPPPVQQISELAPVQAVAFDKRQDLVELLANLEAFKAKPENEQVKQLQMMLYYRIKAKLFQDTEAQWKKVSEVLSDPSNYTIDEILDMIKNEDQFNELVDDAVAQLKEEAGDSVAQQQNQQDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZP6</accession>
    <name>A0A8S1JZP6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZP6"/>
    <dbReference type="OMA" id="FCQIICL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="84" mass="9836" checksum="99945FCA9C05AD63" modified="2022-10-12" version="1">MGVCQSQKNSSNLKRSGAQQFIFIDFDKLKKYNEERQNLDKNNCQQQSFCQIICLDNNNNNKSSNPQTQTYNNVQTQQIEIKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0T7</accession>
    <name>A0A8S1K0T7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0060130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0T7"/>
    <dbReference type="OMA" id="THGSMDD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="550" mass="65742" checksum="AEC6C30F8B99ABAA" modified="2022-10-12" version="1">MNQSMTIINSIKRQSSILSLDKQTEENGTTKLMKKITQKQTHGSMDDISRLLPLNDNLINVYNDFEDNYRVVRESNKFKCEAPQTSKQEIIQINTSNILKVADGLHLCDLNPEKLNRIQIYRFRFYYFRARVKNKLNPLQMYFTFPDKTQNQIYKVFLSTTVEFPTKFNCEQSTSSRSIKVKTKSGTKFFIEDYIYITLYAESDFIITMHLVFGEFHNSILSAKQQEPQRYYKYWDEDKKSMSPKKDKILQNLDQSRYKSTQKLKDFLKGAEVSAKKIITVVQKGKQIEKERQEERSIKMLVKSQIQEFRKVEKSILQQKFDKQYQCKQWQQMISLIEICKQVYNILYERKRRLRIQAKAKLIVLRIKTKLFIEVQQFGQNPFERCNNKSLLLLKIISKHLQENSKKRAQKIATDFLKKTLIFQTALIAHHKMVSKVRMITKVFKAKKFQKRAFKDRFWRLIKNYFSQNNGPQLFATSQVKVIQIDKPLMSKLIEQYIQNRKLQWSQSYKQNIQSDEKLTRIPDIIIFQLPNDKELKQIMKEYYVQKKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K127</accession>
    <name>A0A8S1K127_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130303</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K127"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8049190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="189" mass="22664" checksum="EE7C05E7D9E8B980" modified="2022-10-12" version="1">MNNIKRKIFFIKIQLQLIQIKSFFPSIFLHNISQRNLIIKYIQGVEIVTKRLSRKSVFTSNFLFQKHLNEIVLISIINIINILTGEKMMIKFIFEKIEDFKFNELLYNLRQSKKELVIVNNFQTPLFPFIRRNVQSSETIWLIMNVEQKNNQDNSKFKDQVLRKRREEINNISQYSHIGESVPQINKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1G8</accession>
    <name>A0A8S1K1G8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0130228</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1G8"/>
    <dbReference type="OMA" id="FECEKLM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="486"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="709"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="711"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8049099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="911" mass="107379" checksum="B4E9B266E2C29C06" modified="2022-10-12" version="1">MNQKQSDLLSVTPRVLSSRSSQPNEQLLEDTSRKPSILFTKLEKVQLSHEASESVQSNESQEMPQKFVQGFKNQIWKERALNVIVLVARFVTYLLTNSDKFKLRYLELRQFKVIGDQASDYNYYLTRRLIRAKSKQHICFHLKVVFKKIVHIFRRLGINISPITPDHTLKLLWDLFVFTILIINIIYIPLKISFEIQGSNDGIDFFLETLPQYVFICEILLNFNVAYYSRGILVLNQVQIIKHYLKGKFITDFIVLIPFLIGRSNVPYIEFVLLLRVSRIMFIFENLVETLNLRVNFAAVIDLISLLATFLFASHLIACVWHFIAIQEHLYQNTIYTWADKAQLESDWISRYITSFYWACITTLTIGYGDITPVTQIEKLFVIFVTLLSSIIFGYTISSIGAIFTQISENKNYLRDKMTMIDSFIKRRGLNKELQVRVKKFFEYYLKTQKNTDFECEKLMEHLSGTLIREVKIDFYKSLFFQSKLFRQNFSDEFISNLCLLVKEQSFVPEEVIFQEGQQVDRMYFILKGEVEANVRHNKLVKIYKRRQAIDEKGFISQNCAQFTSRAVKFSKLAYVTFEDVLSLLQLNKEDLEKYYRIKHQIQFGGRIKFSGCELCYQNHIFTKCPFVFYTPNNMRLFRKKHNQDQQNRQFQQRKISQKLFTHKSIINLRILQKSILEYGNSTFLYTDLLPDCDFFQINKNDELNCDGDGQSISEESMNSPDNTGTQFKHQYSSQSQQANYMAQSISKNLNRVYQEPGISSLRIKITKGTKKQTLNASNQKIVHDNEDQKEINLDSKQIILRQNSKQLQDLQSKSTKEINFEPTQIKLIEQEIVKNIFEQHEHQIDSQKEFEFYDIQFNLSNIIKDLFTKKRKKMMFQISRKRQKKQLFCVLNKREQSQIAINNSFCNVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2B7</accession>
    <name>A0A8S1K2B7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2B7"/>
    <dbReference type="OMA" id="IENDMPP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8049349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="232" mass="26653" checksum="193CBA4AE934DE64" modified="2022-10-12" version="1">MFEGTRLQYIVPGYTGHIPKSFFEQQVGYYQEEKPQNHIPGYAGEIKSMKAENLFAQTYGKITYQIQHDDYYKGQDVPPELRYKSQLQDTYQNQNKVQLRTAAEIVGVKPKPIEYKIPQTETAKNFFKLDEQGNPRLKESLSNWKSEYEQHSETIDEATHKFHGDPGQKVPIPLGDPLPGYTGMQKRVVAANIFGQTFANARKTALQDDAKIKDEKMNTFKQQASFIPALKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3D4</accession>
    <name>A0A8S1K3D4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0110395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3D4"/>
    <dbReference type="OMA" id="CEDFHAC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="297" mass="35504" checksum="2201E72FB12F7983" modified="2022-10-12" version="1">MKVKIFLGEYTYVYRGSIDLREINEFVKTLTNRAFIIKWKDQEDDLITISMKQDLVYFYQQWTQNQSTNQQSFKLFVHEMPLIFQNPQTTQLQSQSQIQSKLYSPCQRQSYSKQNQGISQFTFGLQLNEQAKSQEFQKERHSQQINLQQIVEIQGSSKNISNQNLDKIQQQNYMNEDGKRQRVLQDFNNINSQDLLFFMNMDMKQSINKKEFTEIQMATDDDDDDNEIIDNNKDNDMQIIDCKNIYCDYCSDQIQCNDELTIIYTCQFCEDFHACKQCFDQYQEQVHIHQLKENILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3Z7</accession>
    <name>A0A8S1K3Z7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">mRNA m(6)A methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.348</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0140059</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an adenosine in mRNA + S-adenosyl-L-methionine = an N(6)-methyladenosine in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55584"/>
            <dbReference type="Rhea" id="RHEA-COMP:12414"/>
            <dbReference type="Rhea" id="RHEA-COMP:12417"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74449"/>
            <dbReference type="EC" id="2.1.1.348"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MT-A70-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.348" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001734">
        <property type="term" value="F:mRNA m(6)A methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF7">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="35"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8049577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="246" mass="29172" checksum="2CECA3C5D2D1BB83" modified="2022-10-12" version="1">MRRRVMQQEDQLKKKSKLVIDNSDEEEICKKVKLEDPSYSEEEEEVVDVEELEESEEEEKAKKNKKQETIEDEDYIYVDKLPNSEAMLRDLKKQVEQRIKYYKMKYIQEYHENHTKQSFVHNDAIPICTDVRLMDFQKLRDEQLKIAGQLFDVIMMDPPWQLSSSQPSRGVAIAYSSLADENISKMPIETLQENGIILIWTINAKYKVTCKLIEQWGYKLIDEIIWCKKTVNGXFFFLCFTVLSFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K713</accession>
    <name>A0A8S1K713_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0170104</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K713"/>
    <dbReference type="OMA" id="YQTKNIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="217" mass="25459" checksum="FCB58A0514A9A562" modified="2022-10-12" version="1">MINQINHNSSEQYQNELTSTKTNPYQTKNIQDASVQVEFSNAQLMKIIDEIDKIHQQEKKALLEQINYLEKKLQQTKAKQLILCINEVKNIYLNQIEINKPTRMALDSLEQLINEKQHYEVSDLKYRLHQAEKVLKLPMAEFKTYPVEKLVNSIKKCQQFESENTNLANILEELNQADDHWNQIQKDLYDESSTLVNSLQTTTQNIVYELSLLDCNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7R4</accession>
    <name>A0A8S1K7R4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0180138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7R4"/>
    <dbReference type="OMA" id="NDKTWIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8051520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="367" mass="43872" checksum="6E1C89B67B8A2757" modified="2022-10-12" version="1">MNCFRAFVIYDIELSQFYLLLRRDIEFNSLWQQTIKEIQHQIIEQNVIEEKVFFTHSLLGKVRGKYDYFTNRLLILISHQYVDDIFQAELLLSIYAYLCEVQNYKNLKIQELEALEKFKVENLIKIKEEQLKMNNTFNNSKYSSLKPNLVDQQYQKMLTCTTLSSENNEIQSYSSKQLLDEQYMKVFKGFMLYDKNKQYFLMFVRKGFNNDKTWIEERVRIENILLETTQKTSQIFCIQSKFGQFIVEYDATIKFYFILLSRHKAAENPQYQLLQRIKCFIQLEPKFHKQKKGDLESKLLYAINDIIDAEERIYVQRYGIQYLDSKSLRPKCQSEKTSVQPSQQQIPSISVIPNIQDCNSLQQIQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KBF3</accession>
    <name>A0A8S1KBF3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KBF3"/>
    <dbReference type="OMA" id="RMDYDTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF151">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR MTERF9, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="385" mass="46223" checksum="1FDC93A33D6E2F94" modified="2022-10-12" version="1">MKAIFFRFSSYAKFAEKIQNTKFATIYDYETQVKPTLNIYESMGFNEQMIFRIFKNRGLSIVKGNTLNDIGQLNELFKNRLNAETKQMRTILFKDPQILSHPIEKMNNYLDLFEKQLQIKKEDILSMLTAYPLLMRNFEVNYEKFRNVFNTYAHINDKQFGQILVNTPFLFSFNLERIPPNFRVMYNRDYKTKEIQELIQKTSEFLALKNHDLDRLLNHYDVLIPNKEVQHQLLINNHKLLLLTPAYMLSPKINLFKELGLSLNQIGQILQICPNLFIKSVQTLKLKLKFFEKHLNIKIKDSQYFPQILEFDYWTVLRPRLVILNNLENAMDNLKMDQEEFIKKYDCEQKYQELLNEAPAKKKIDADFMIRKYHRYCHDRIQLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDN6</accession>
    <name>A0A8S1KDN6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0190164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDN6"/>
    <dbReference type="OMA" id="QKFQFPE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="451"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="252"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="173"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="451"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="466"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8052401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="539" mass="61803" checksum="894FFE75FABDAC30" modified="2022-10-12" version="1">MNPNDEQQRLYQMFQQMAGPDPYGRMLQIPNSPYYPCWGPPFPQPIMAPPPNQKFQFPEQFPHDQALQMNQLPSNQNGLNLNGITQGLQNMPPMPMGLPPGFGMPPLGPGQIPQGPLGAGSMGPPPMSGMNGMGPGTMQLPGMGPMPQGPLGQMPPFDFQFKQQSPLQPPMNPNQVLPPQQQPQQQQHLPQQHQLQQQQQGLLQQPQQQPQGQSNQPNNNAVQQNLEQGFSQVVNEFQLKILNMFVMQNQMLLDFKNKNANLENTITQILDEITNLQKIVEQKFDNIQADDFMSRLSIPNQEMTQNNILLKSLQCNQNDFKYQLVLTNDLEQPLFKDKNFNLDIALKDMKGINIKNHNKIELEVQLYSSDDKPVLLISNSQNQAILRQPEDNIWLEEGISNIEKLQINEVTSHYQNGWIYLVVYPIKNDNKNVQNEINSAQIKPLIYKVSVKSKKTQKKSSRSRSRSHERLRRLEKLDNKSSSESENHIQLEIKNLDDNDEIQQEKDNDINKEQIEQKDQQQNDEQKKDEVYQNNESEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KI11</accession>
    <name>A0A8S1KI11_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0210155</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KI11"/>
    <dbReference type="OMA" id="EACINAP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902494">
        <property type="term" value="C:catalytic complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098796">
        <property type="term" value="C:membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000022">
        <property type="entry name" value="NADH dehydrogenase flavoprotein 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1590:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01958">
        <property type="entry name" value="nuoE_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371:SF3">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01257">
        <property type="entry name" value="2Fe-2S_thioredx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="194"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="214"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8053873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="263" mass="30009" checksum="2A5D0C02E2C3678A" modified="2022-10-12" version="1">MIQKVVQLVQRRSIQRFSNVFVAHRNRDDNSDSVPFDFTDENYKKIEGILAKFPTNEKKSGTIPLLMLAQKQNNNFLSLSAMKKIAKILEIPEMDVFETASFYSMFNRERVGKFHLQVCGTTPCQLCGSKDIIKAIEQKLNIKNGETSADGLFTLQEVECLGACANAPMMQVNNEWVYEDLTPDNTLKLLEDLKNGTDKKGPQNSRKNAEGPQGRTTLQNIANQQDIKYDRNFEAAKKEWLDQKEKERLEAEKKKQAAQQAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKT6</accession>
    <name>A0A8S1KKT6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PPRIM_AZ9-3.1.T0200352</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKT6"/>
    <dbReference type="OMA" id="CMELCAG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000299">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000383">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000828">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 27" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="26"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="330"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="368"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="401"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="437"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAD8053482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="473" mass="53924" checksum="3C4F05E88CB45D3B" modified="2022-10-12" version="1">MDQEVFLKKQWFIMTHEGKIEQFYDINLKKVIGSGTYGSVVKATLKGTKNQRAVKVIPKSKVKNPDRFKKEIDILRQLDHPNIIKLYETFEDQRNVYLVMELCEGGELFDRIMDKGYFSEAEAHEIFLQIMQALNYCHTNGICHRDLKPENFLFLTKADDSPIKVIDFGLSTLFEDPISQKASGNQKVTMKTKAGTPYYISPEVLKGSYDESCDIWSAGVILYILLSGVPPFYGDSDPEILDAVQKGEYTFEIPELKQVSDSAKDLISHMITTPEKRYKASQVIQHKWMKDGSKNTKQLKLNFGQLKNFNGSNKLKKVALTFIASQLNEQEIADLGKLFKQLDKNGDGVLTIDELREGLIGMSDVQAKELGNIIKSIDTDGNGTINYTEFLAATMEKSLYMKEEKLYQAFKMLDLDGSGKIDKHELQTVLGKSEKIIDEKYWDDMIKEADKNGDGEIDYNEFIEMMDKFSLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KMP5</accession>
    <name>A0A8S1KMP5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210358</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KMP5"/>
    <dbReference type="OMA" id="NIHRVKK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="169" mass="20240" checksum="7C714296A85022E7" modified="2022-10-12" version="1">MIPGGIFSQCLQEIKENCPPLQRVNYSHKKSFRHCLESITQKIPIVKSIHKLRLDQEQKIYQNIHRVKKLNLMHEIHQVNPKLHNELKQIQHDQLTLKFAEYKQQQEQCAEILSDTQDSYIKYQQQLDHHGSGLKIFHNRPLIRNIMEEYDQEESTYSDSYINKYLDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTS8</accession>
    <name>A0A8S1KTS8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0240150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTS8"/>
    <dbReference type="OMA" id="WAGCNQP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8056116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="306" mass="36280" checksum="AD2F3083F07B74DE" modified="2022-10-12" version="1">MILSRGLKQQLRQFTIQTFANTTRVPNPFAHHHHHHGHEHEDHGHDNNNHGTKHLGNRPKVEYNTITNFLDHKNLYTYFEQGPGYMYYPGVNYNEAHSKPYDWRDDPKHNPDYLIPTKHINCPDPKTYVWPFEGRLEPFNPSEALSQNHLYDSSLLLKPENKQVGQPIHHLTAAQQDQDADVNHEFDYESEDCDFQTESFRTQHFRKRGPMWPWALIATMPFVYFWVEFLYQRYPDEDHWRITHPPPLNYPDSEDTDDTDTYQDYHSPSGRFLRDIGIIGDLWFDIKDGKKVMNQWAGCNQPLPDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUT3</accession>
    <name>A0A8S1KUT3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270306</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="264" mass="30611" checksum="1538B2F068FED8EE" modified="2022-10-12" version="1">MSGKDFSQFLSNKDNQSIQESVKKSIDPNLFINEQTLKQIEAETNKHFWINYFTQSILTTFIAPLTTIYTSLQLSVIPHQNKYGDLPDESGTKTSTQLVKTTRDIQPHEKRKFELLIKSGALLXYEELPEELNQQEFYEEINDIQSDQIEFDQSTDDDSDSEDTDNQNIPKADLLEVQQNEMCFQAFSECDFQGASLKICGPNPSIPKELQNFLIQSIKMPDQIRITFYTNQQQKITIEGDQECLQRPFEIDQMKPQRQLLSHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0L7</accession>
    <name>A0A8S1L0L7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L0L7"/>
    <dbReference type="OMA" id="YYSNNHP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="227" mass="26959" checksum="9908C273C91B7F4B" modified="2022-10-12" version="1">MKSIVKTEEDSPKKPIWESLLERGSCKKQEIIKKIEEKKNKDEELDPECTFKPFLMNQNQLQLDKPFYERQLKWLEDRKQKIKERIDGAKDKSLEKCSFQPQLGPSPQIPEVDIRNTKGMDSFIQRQEKARKLKQEKQVLLNNPYSHLSSEKKAVLTNQSYYSNNHPISKEYESIKKQERIEFSKAIENSQEIETQSQTQQQQKMQLGMAMKMLHNQLYNIQFNSEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L134</accession>
    <name>A0A8S1L134_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0300222</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L134"/>
    <dbReference type="OMA" id="QNYEFGK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035856310" description="Trichocyst matrix protein" evidence="3">
        <location>
            <begin position="16"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8060611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="40578" checksum="0034EF21BC76CCC3" modified="2022-10-12" version="1">MNLSLIYFVIVIVAANDYELLAQLQNYEFGKTIIATMQITLASEDASVDGVVNQLKAMQKATQNDLDDNSQSIKQSQESCNLRFNELQIVIENAQNKKSDNERTLPFKQEELNIITYQIKDKLDQQNRIQDRRNGAEFSRSEEKKDFQQKQQETLDFISGLKKAKAILSQLKSSFLQVGDVRAKLHKHLESLNKTSPYHGLVKILIAAAGDDNVPKIIQIIDELVESLQHLLSVDRSGDEQKEELYQLQKNRYNLELQTLNTNIASLQAEAEKLKQKLLEFQNDIESKEQLLTVKNQEYNDWKKTCDDDLRANQNLRQIKFNELNIINECIEIFTTRLNPQIKSYIQELEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L192</accession>
    <name>A0A8S1L192_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha-soluble NSF attachment protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L192"/>
    <dbReference type="OMA" id="TEIRACK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15832">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF8">
        <property type="entry name" value="ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="33938" checksum="2BD83A3546B55C93" modified="2022-10-12" version="1">MDSNYVLKGDEFMMKGDKALKGSTFGNIFGSKGERAEKALELYKSAATQYKLGKKWEKACEAYQRCITCDQTLKSGETGDFYVEAAKAISNVNKAESIQLYEKAIEHYANENRLDNASRYKKEIAQIYESEFEFHLAVKAYQEAADLFQADGKRISDYNQMRLKVAELSTLNASGDLIAAIKIFEEIGDKYMENKLTAPSAKELYFKSCLLYLCNNDSVGCGIALERYLDKDPSFGSARQYKLIVNLIKAVDNNNVQMFSDECFEFNKIIPLDKWKLNVLNKIKEGMQPIQQVEDGGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1A6</accession>
    <name>A0A8S1L1A6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0310312</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1A6"/>
    <dbReference type="OMA" id="VDLECND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF56">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="294"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8061439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="518" mass="60633" checksum="4DA028594F38FA62" modified="2022-10-12" version="1">MSIDLSFKNLEILDQSLNLNNYIIINLQGNNFKGEQSYIQQIKVQHLNLSFNNIEQMWLLPSSIITLEINNNLIQNLSSIENLGQLLDISHLKNLKQIDLSNNLLQSVHWFSNLTQLTHLFLKNNKIKTIEQLKNLPQLIEVDLECNDLKNVAEVHYLETQSKINIINLFGNPVFEGLQLKVLNVVEKNCHCRSEQIYNQSEICKKRNQQIDDIDLFDEQANEKQSDLQPQTLTSKKYTKSLSPLNKQLPQFYDPKSSKYVTFHDVLRQKQRNITNTNYDVKQQNVTTTNTNMTINNRQTMDQLKQQNENLKKDYMKLKQKFEQSQKQNQSFYQEIESYKEQLNGLLLQFNIPIQEEEASVRSLLDQLELGLMTFIKETRKQNIEQINSMMTRMGNKVKVMSSQIEIITLKKCCEYTEKIKNIELSLSKLADIVVEQKQQILKLQRQRVHSPLQRQCLTSRFHGKNPSELDQMKLKTLFFKSPNVQQLDKPLKTQGSQITSLSQQMLDTQQKPKNLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4H0</accession>
    <name>A0A8S1L4H0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310151</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4H0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="35295" checksum="8D9EA6E3F9A90135" modified="2022-10-12" version="1">MRNYQSQHKNHMKLTTKTKQQFKKLFLIKKDNLFSKLSQKTSSLITCFLNIQEFNTLLLVSKTTYQIFNQTSGCFQIECLRLLNVKCELIQSQNWKILLQSVMCIPIRSLPYGEAIKSVRYQIKQFPIFIEQKINYCGFQKAFLYYESQLSEKKKFIKTVDYRSALHFASNNTDILLRVRQGFEIHTQLPELLILIYNLHDYVKIFCKYLEIRFSKCNLEQFIQIWHHYQGWISSVENQTFDLIFLFNKIIDESLHQYKLPKYTIRHFMVCEWMKKANKGELIVKLREDFRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L547</accession>
    <name>A0A8S1L547_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Toprim domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0290211</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L547"/>
    <dbReference type="OMA" id="FLLEWIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027032">
        <property type="entry name" value="Twinkle-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12873">
        <property type="entry name" value="T7-LIKE MITOCHONDRIAL DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12873:SF0">
        <property type="entry name" value="TWINKLE MTDNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8059876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="414" mass="49028" checksum="511A380038FEB985" modified="2022-10-12" version="1">MLKKLSQQYQKISMKSLQLGISLNYSTNKIRYSFRSTRFIVKSQEQNESSKIMMITKEEIISFLKNNKIAYRIQRTYAQLQFCPFCIKRNFDELSNQYTMGINLKNGMYNCFRSSCQSKGQWKDFLQQMTNNDPKLIVDGPIHSIPKQNNQEEVGIYDQKYELFQFHLHYQRMKLQQPKFIYEYLVGNNWDKNQRGIKEEVLNKYLVGIGQDEVFDPQLKQMVMAPLIYFPMFRLLSQQQQNDNDIKNEYIQSKYNSQLYELVSCKLRGMGKENKYIQKIEPKNAMKGIFGLNLLTKNVKQIILTEGEFDAMAAYQMTEIPSISLPYGIAHFPNFLLEWINEYKELNNIFIWVDDDEFGRVNSNKIASKIGNWRTRIIQPSLINNNQYPKDANDCLRYYPDKIQEYISKSITVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L719</accession>
    <name>A0A8S1L719_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L719"/>
    <dbReference type="OMA" id="SFNICHS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000471">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039245">
        <property type="entry name" value="TYSND1/DEG15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21004:SF0">
        <property type="entry name" value="PEROXISOMAL LEADER PEPTIDE-PROCESSING PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21004">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="489" mass="55607" checksum="538B8782E6EE322B" modified="2022-10-12" version="1">MEYNGGIIRIQSQHPKFLSLLQSLDSQGTYSNTIHQQNCTFHKFQENHNLIISTGYLIAKFAQAINSYNINIIKQHIKFSLILQDNKEINLNLICLNFDDWIAEKLNNCYIEIELGFIMYFEYPNINIVQQIPKYCSSLLTQGQEVLTINQSPFLKGSVMQKQYCRKAYVSQLFTNQNQSKQLMMLGIVSQVGQEGGGIFNSKGEYVGILLQNIKIGRLNNRYFTFCVNWVVINSIFNKNTPQLNPSYFNAEIINQILNVTKYISQGAGQFGSGILVNDLQNQQSYILTANHVLSDILKGSKILIGEEQYEAFILEDTLNNMDFSIGKAKNYISQGLNIEDDVIFSEVPVVGSTVYAVGYLYTLFQYKPCISKGSIIKIIYDQFNQMISIVTDCFIHNGYSGGGLYNQYGQLLGIISFNICHSIEGVICDLSYITPIFPFLTVFQDLVQQRNLTQTSKDKLKQEIKLASEIRTLCTLRELPQIGYIPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L7J3</accession>
    <name>A0A8S1L7J3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0330035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L7J3"/>
    <dbReference type="OMA" id="NNFKRNM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8062235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="313" mass="36948" checksum="D6084AEDBC885CC8" modified="2022-10-12" version="1">MFNNFKRNMRILNKDTVETSWLKCLIDDIPKQKHILILSRCLDGEFNNVNSQEALKKIILRMQHPQKTVTALKSYYLVHELRQFLVPYVTDKTIELSQKSTADAVLQSVAFLYCSYLKNFSNNYEQINIVYKMHRQLEVLILKLDVPIIKCITIKLIIDIIFLYESLNKQDDQTKRIRAIKQIKRFLEIKKLIQLPEELYQKINTLNFDEIEQKITLQKQSIITNQVENKLNAPKKEVLKLDIKCINQRKLSTSANDWEDQLDFKVQEQQFHKSLQPSPNRRRIASQTPNRTTSIRDNFFVPSQGPLSFIMMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHR2</accession>
    <name>A0A8S1LHR2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0400252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHR2"/>
    <dbReference type="OMA" id="IAYEFIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="357" mass="41677" checksum="0939EDD850EEA626" modified="2022-10-12" version="1">MDLFGLVQTAVDLAKAHEENGEEVKFFRLKLSSLQEQLQKLEQKDFQINAQLQGQIKKELTESSQLLSKLPERNSYINTILSKLPFGSVYNDIKDKSQALDRLSQQLNNHLITCLALAQTSTVEKQGNVTTHVEETQIDDDQIVQINQSQGKIQLKLIAEDHIKQQFRMNIINTIEVIELKFGNNGNNGNDEQEVFKFGREYYRKLQMEVQQVQTISREHAIITCIRKQKQKFDSDNFEVPQKKIKIDKQSEQHQVQQQEIFYTCIDNFQQQEEAKPGDDNNNYEYIFKLQNKSPNGIFIYRDEEQTKNNDDIILGTWTRIVNNKTIVLKSKDKIAILMKKPEYKQPLIAYEFIMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIV0</accession>
    <name>A0A8S1LIV0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cilia- and flagella-associated protein 206</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0390237</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CFAP206 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIV0"/>
    <dbReference type="OMA" id="QYPETCS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003356">
        <property type="term" value="P:regulation of cilium beat frequency"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021897">
        <property type="entry name" value="FAP206"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21442">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21442:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12018">
        <property type="entry name" value="FAP206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8066669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="602" mass="70120" checksum="E7882311A37A4A2D" modified="2022-10-12" version="1">MNQLDEVVKFALYQCRARNNPITETLASYVAQTILNKRTNKFYLEEKLNESELNELKNETLNKLSNQNAPDLKTIQLQIQYDSAYVEMELQRQEKIKKQSNETGKFIDDVVTLEIKNAKDFEGLTTLYTKMFHYLIYKNKELIEDPINFDAQAQFNMDKEVGAALESVIPRAALGPFVSLNPSEKVTQLVELANLVIGIRLFNKKIGKGGVSLSSLEDLTKYNARELSDQIKQIALETIEQCEIYTTFFINQEKFQMNNEDIEKYKDELAFLRQYLSYILSLQEDIEQSEAMIEQNRIRFIKEMEDLKKLLEHKSSAPKEQVYPKFALLAQSHIGLFEDKQISIERVELFRLLVDLRKMMKLSMPLAIQKQCKQMESTQPDNLALINYQPESGVQRLLPQNTPDFMQTQLDYFGFCIWSVVKKNGLLIPGKPSLGVFRYRDKNCVFSNEIAINEFLSEPQRYLTGVIDICRQKPHLIRLFRVEENFKNLNLKLTFESGSLLSNKLMVDKDVQTPVHFIEKNLDPNYCWNEWELRRKAIQMANIRKRQTKASQTILSNFKVDSEAQVYEMKDQSTSTGQNKGLNPLRPRNYIVGLRDKTYNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJU3</accession>
    <name>A0A8S1LJU3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0390085</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJU3"/>
    <dbReference type="OMA" id="ISEAWFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF35">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08182">
        <property type="entry name" value="Pedibin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="261"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8066432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="490" mass="58131" checksum="A24322B07780899F" modified="2022-10-12" version="1">MNKTLERNQNGTVIMNLKNLKIICEREGLYQNPENNDTLYLHFKGFDKIENLEPYFNLVALWLNNNALQKIEGLCQLKKLISLFLNHNLIDKIENISALQDLVTLNLSHNSIKKIENISSLTKLQNLNLSHNLLTNYQSLLEIQDCPSIQNLDLSNNHINYEESIITIFQQTYIGCLYLKANTFVRECPNYRKTIIVAIKALQFLDDKPVTPGERKISEAWFIGGKDAEQQERVSQIEIKREQDHQIYLQTLERQQKGEIRNQLLNKQQILCKEIRQLQIQYFDGEEVDDLIEQKENELKNVEFELDKYQVDTILPHQCFLTTKDDEGNTVILNKTEDEAKQIRDRYFNQEMQQINQKCDNNNVENQLNNQKYEQQEEQEECLQSNKQSKFIINLDSGPQIPKVDTKAQQLREYKQKREGWTIDHDDLLENLLMYHQFNFDAVSDEFKRIMAYEDQELKYLEPEDLRIIWTYIELSKYRNKIPQNLDRIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LK33</accession>
    <name>A0A8S1LK33_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0390296</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LK33"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8066785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="66" mass="8015" checksum="A77D5C98E11C0069" modified="2022-10-12" version="1">MRNAKSKRGFYVSAEAKFMLDIRNKRILNMNIQVLILQSHKSHKILEFLNQDNFIMMFFQNQHSHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPQ3</accession>
    <name>A0A8S1LPQ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AN1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0420117</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPQ3"/>
    <dbReference type="OMA" id="DQMERCD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677">
        <property type="entry name" value="ARSENITE INDUCUBLE RNA ASSOCIATED PROTEIN AIP-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677:SF20">
        <property type="entry name" value="ZINC FINGER AN1-TYPE CONTAINING 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8068355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="136" mass="16423" checksum="E0AAD29BD85AC0C5" modified="2022-10-12" version="1">MNLDDQMERCDVEYCKRRDFLPFKCTLCNKKFCLEHKELKEHECPFQFAERKALKCNQCNQVINYLSTQFEIDVLKQHVCQKINVEKSRCPQCKITLNDLNKFHCTSCKRLVCLKHRMKDDHECEKYSKLNYCILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LW48</accession>
    <name>A0A8S1LW48_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0440260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LW48"/>
    <dbReference type="OMA" id="YSEILCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8069831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="504" mass="59041" checksum="3E858075B3A10DE7" modified="2022-10-12" version="1">MSYKPISNDQHPLDIVDDDGELDIVLKRQEKPRPSQKSFLIWIVIVTFLGAILIYIGQSNSTGTGQNKIGKNKIQNYTKVNKTIIPDHNDKIIESKEDQIQLEDNNLIDINMRNDSQKKSENINQTQNQTTKVEDKQENQEEENNIDFEELQKQTDQLNKEIKEYYKNKNETQNVDIVNEVQKDPRYGEILCESGYYMNKDEKKCKKCEDRCSECMHIIGTCYKCQNEYYLNNYGKCIQKCEGKHDSSNLLLSDDNKNQSIPICIGNNHTSSLFMSVQKSNNTHFVLPYLIIHPATTVSLMYHNNYPLVYYFNKDIFNFEDEMKLNDIITQAQEKQLIVIIIGSHTLYDFSLFNNFLFQEALQNIEHLVKAKSSTFRSFFGQDYNAYGVIREYQSQYKNNSVDLVISDDPIEIDHEYLESNLLVTHEQHYTDYNFESLQGAQSQKTTVILLFHTKNEQDQKDYCSRFLYVSFLQCAVREIQIDHIQRMRQGFELISQFVVQPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXU1</accession>
    <name>A0A8S1LXU1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histone-lysine N-methyltransferase, H3 lysine-79 specific</fullName>
            <ecNumber evidence="1">2.1.1.360</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Histone H3-K79 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0450179</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60328"/>
            <dbReference type="Rhea" id="RHEA-COMP:15549"/>
            <dbReference type="Rhea" id="RHEA-COMP:15552"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.360"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.360" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXU1"/>
    <dbReference type="OMA" id="IMKQSHS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140956">
        <property type="term" value="F:histone H3K79 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025789">
        <property type="entry name" value="DOT1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030445">
        <property type="entry name" value="H3-K79_meTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451:SF19">
        <property type="entry name" value="ACTIVATED IN BLOCKED UNFOLDED PROTEIN RESPONSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451">
        <property type="entry name" value="HISTONE H3 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08123">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51569">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DOT1" evidence="4">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="492"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="256"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="568"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="492"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU271113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8070341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="781" mass="91825" checksum="17DF88002AEDCAA9" modified="2022-10-12" version="1">MDHARILNFLNSLQNQACMIKQEGRIDTQQTDNAQCPKESLKSLYEKFTKDYSLQRARTLSREERLANHLTSNSLSYGEIEFTSLSVVFQQVKPKKGGVFYDLGSGIGKSVIAASLMHQFDICKGIELLHSLHEQACKLKQEVEKQKTLIVDEMEQIGIKEYQQPKIEFINGDFQELDWSDGTFLFASTTCFEPDLMKQLSKKAIDLKEGSYFITVTKTLEPQTSKENELDLTIIKIIMKQSHSKKNITNSLYIANNQHTELIDYLKQENKQLTEQMNELKGLLQLNKKALRIMTPQNNDEQNRALLMVMKNLQEENAKLHNQIDKLIEERNQAQNQVLINQQITEEAQRHEKELILSLQNKIQTLQNNLNKTEQQLSKMEQLKPEYDEISGIVIKYKEICEPDKIAIKFHNQIEMLTYQLVEQIKKNKVIEAEKQKLQGFNLKLMSNLVQMKNTAMTYHGNKLIIQQQQIQQGPEQLIKFQNNLLNVMNKQEDENSSQDDKNSSVGSSPLMSPLPLKQENQVVKQIKDIPKLDLTRAKQIQEINAKRQIQQMQDIKKQIDPNFVDKLNKYERIIEELRKNYQREMLLNKTLETANNQLQRNCDDLENQIQILITSNIRHQEKLQKINKQYYFLQQFYINNKDQITTTQSHNRRFSQSPTELSQEMILDTSFIDANGAIISNEIQEQKYLQQQMFEQQQQQQQFQQHFYSFYSPNNIEDSKKFLLSLAELIYCGLQDKIEQLQQQQQQYQQEKKQQRFRSVSDIIDYQHIGFHKYNYIQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M428</accession>
    <name>A0A8S1M428_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1772 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0490211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M428"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013901">
        <property type="entry name" value="Anthrone_oxy"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36535:SF1">
        <property type="entry name" value="DUF1772 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36535">
        <property type="entry name" value="YALI0E30327P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08592">
        <property type="entry name" value="Anthrone_oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="16030" checksum="3F21953AF93D1D55" modified="2022-10-12" version="1">MDTQTKLTLLGTAFAGLFSGASIYINVVEHPARLSCGADVAYNQWVPSYKRAAKWQASLAFLGGLVGIINYAKYQNKLTLKAGLLLASVIPFTLIAIMPTNKILLEQTNLEDDSKIALLKQWGKLHAVRTVASLISFGIFLYALAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M865</accession>
    <name>A0A8S1M865_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0530271</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF-assemblin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8075002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M865"/>
    <dbReference type="OMA" id="DNDSQRE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008374">
        <property type="entry name" value="SF_assemblin/giardin_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06705">
        <property type="entry name" value="SF-assemblin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="172"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8075002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="249" mass="29941" checksum="31CC884437B195B8" modified="2022-10-12" version="1">MSENIQNRVSSIFEKLNNISTSVQDEKNNRFHTISQLIMAFEAQLQHQSDQKEEKFAYIAQKVRQLTEFLEQEQEDRERQESETFKLITDLERHARRLIEQNSKDRVEQEKKIVYTIGQQIESLQQEVVKEGLAQSTSHEYIDSYLNEDLPKIADELQNEITERKDVEEKIYHQFVEQLNDLRELFEREKKEREAKEEEIVESLREISGRIQEQLKKTRNEREKTEETLVQLVEKVIEKLKREMLEMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAV9</accession>
    <name>A0A8S1MAV9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0510199</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAV9"/>
    <dbReference type="OMA" id="ITPKESM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38394">
        <property type="entry name" value="NEUROFILAMENT LIGHT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38394:SF1">
        <property type="entry name" value="NEUROFILAMENT LIGHT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="297"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="450"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="759"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8073706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="820" mass="97596" checksum="A492992801E97A6D" modified="2022-10-12" version="1">MFSKFKNKEHQHNLQSNPTTPPKQSTSSNSQQNSFVPQQITPKESMFKGSLKVKTPQQTFPQSQSNLQQITIDTQQQTNSPIVSTINSADNLQTQIIEFPLDQHNVDQNVEIKIDDAHFKKATSGFSFLKRVKQEKSQSTDESTQDQNESFIVCGMNSQQNSTQIYPQDQIVFSFQNYDNTNQQVNESNQQQQIYTNHTPIKQQDVQQIIDSQNQKKLIQNMNTQIDDSHLEQYHILIKQLHCYQQEQDKKQSEIFLQQQKMEQLISLNYQQLNDLYIQLTKLSLEEQTFFSSEQFETSLQEEYVEIQNKIQTIEKQNKMYEFQFNNFQNQKLKIKSDEQIYYKIIEQQLDEIEKIHLKYIEQYQQESTTTIKRLLQLLDEENERLKIQQIHIDINQKHLDQEEQHLNQIMDNQTKDYRISQELLQQKKLQLNTEITQLQLILNQKILELQFADQELILTQDKIQQVQQKFQQQLGKVQNKRTKLSDESESLSVEKLQIENLEIECITKQQDYKQKLQDLQDLLYIIKEKRSNLIENTDLISTQCKLEIKVFHKYQEQKQISFSILQELQQVQRSIDRLNYQRCISQQQLHEILNKKSELQIKLPQMIDSKAQFVQNKNFKGAATINEQIKEVQKQIIIFENNISQNQSKESQLLQQIDEIQDILLLKQQQFKQLNQITEILRYQFQQLKLEQIQQLEYQGLSALIIEIKDELNILYHKNEDILNPNKNQSQDIPIFGKDQVGNNNQILDNDIIQEQYNPEIQQQIQKLKEKVFKYAQEEQFQEADQIQQQINQYQKEALKLNDGVIRNFEMDLREKLIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MB93</accession>
    <name>A0A8S1MB93_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0530282</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8075013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MB93"/>
    <dbReference type="OMA" id="RTSMNYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47567">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE/SOLUTE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47567:SF1">
        <property type="entry name" value="NAD-DEPENDENT EPIMERASE_DEHYDRATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="1">
        <location>
            <begin position="115"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8075013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="32815" checksum="7B5B85FDB97EDFAD" modified="2022-10-12" version="1">MTKDTVNNINTSANTSFNFKEVTNKALRSGLSGSMAMVIQVCSLMWLRTTMNYQYRYGGTIVGTIKNLYGQGGVLRFYKGLAPALLQGPLSRFGDTFANTLFLTLMDSFEQTRELPVMIRAAGGSVMAGIFRIALTPIDTVKTLLQVEGKNGLTILQSKIKVKGVPVLYHGALATASATIVGHYPWFATFNLLNEKLPDYADKKKKLARRAFIGFCSSVISDTISNSLRVIKTTKQTNQNPITYKEAVNLVLEKDGIKGLFGRGLKTRILTNGLQGLIFSVLWKGFEDYLNRRSSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBD8</accession>
    <name>A0A8S1MBD8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0580234</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBD8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001129">
        <property type="entry name" value="ras-related protein Rab-44 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8077680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="299" mass="34966" checksum="7C9B548D95717E1F" modified="2022-10-12" version="1">MMNSSADKTPLFKFIIIGESGVGKSCLLLRYTKDEFVSEYNVTIGVEFSSKTVEVDSNTKIKLQIWDTAGQESFRSIVRSFYRNVTAVFLVYNITKRDTLEKLDGWLKEAKENASPNIVTVLVGAQNDLEDQRQVSYDEGKSFIDEKGINLFFETSAKNNQNVELVLSEFYYMHSPKQPNWFSQTIQMKQCVKMKTKSLLHYPNPMIIHSSSYNHRRKRKKMIQGVVEKSVKPTIHINTYIYVQFHILCIFISKTQYFFQVFKICSIEILIYSISFLFRFCLFIFILLSSYQIKYNLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCW4</accession>
    <name>A0A8S1MCW4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0590153</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCW4"/>
    <dbReference type="OMA" id="CHVFTEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF1">
        <property type="entry name" value="UBIQUITIN-LIKE-SPECIFIC PROTEASE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="4">
        <location>
            <begin position="188"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8078077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="392" mass="46693" checksum="3DEC97DCD899880F" modified="2022-10-12" version="1">MTEELTLMKVKQKIFWADYFNEQYKFKNFNTRKHGVTINNDFLSVLANNQFLTAQHFVFFQQMFSYYYGEFQVLSLDCHVFTEFIPDVTNCILPNMMVTQYQYDSTPDSFQMFEQSYQQEQQQTQQTQNKGDSFFYKISNIFKTLNPLQFIRKSQPPQDLDQQDNFSNQFNIGQQDQMSQQKELLQKYNFQVKNITYIKKDPKEPSQVIAKGQIKINNVILLENEKGKYQFNKLNIQIENLKAKYKFSSQVQKNIFSLFKYTYIPINLQNNHWLCAIVEFSENQIQYLDSNFAKQDNVVEGLEQMLNYKGEQTQWQIIEGSPKQENSFDCGIFCLMALYQLYKTGNFIQPNQYKQQDIKSFRKQLLHLAVIESQSDINQKLLNIILDFKHQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MH35</accession>
    <name>A0A8S1MH35_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bulb-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0600261</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MH35"/>
    <dbReference type="OMA" id="FIFHNQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052981">
        <property type="entry name" value="Ingression_C2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052">
        <property type="entry name" value="CONSERVED SERINE PROLINE-RICH PROTEIN (AFU_ORTHOLOGUE AFUA_2G01790)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052:SF3">
        <property type="entry name" value="INGRESSION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="146"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="4">
        <location>
            <begin position="476"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="286"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8078809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="592" mass="67277" checksum="A45D00BB650969EE" modified="2022-10-12" version="1">MGCGSSAGAVIEQNRAPGFKGPVFMTIKHALLERDVRFIGVMDPYIKLIMGRQEFKTPIAKEGGKHPKWEGCTHTFQKLLGEPDVIIGQVWCENTLTSDILIGEGAFSLTSIRKGNAEPKRISFLLFHNGEKAGEISLEATFTLDPSGKDLPEIKAPDTEGKFIVKPKFGRLKKNTNLLLKMDPFLTIHFGNETMNTSVDAAGHTTPKWNDEIIFTRETNEDTLYIEVWAESLIGWNDFLGCGYASITDSLSLKENQLQTVTIFYDGSNVGEVEIECSFKTAKPQDYLQKDKQSTEAPTKESFQQQLQQYQQPQQQQQYSQQQQQQQQVLPQQLNQFVQNFEARYQNSYGQQPMNGRVDFTYPNGDHYSGDMMNNLKHGQGRLQFSYGGYYEGQWANDQYNGMGILVIGDSKYEGQFINGKKNGQGKQVWNNRQQYDGQWVDNNMHGNGEWTFVNGGKKKGVWANGNRLRWLDDDQNIISSNKTMVSGFMLTSPNQQFTLSFFHDGRLFIFHNQTRQPIFSAINFNRQPIPPVWLKFDQSGGELQMIDAKGMPYWVTGQNFGNFAPPFVLILQDDGRLVIQDANGQPKWYSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MIU2</accession>
    <name>A0A8S1MIU2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0640027</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MIU2"/>
    <dbReference type="OMA" id="CGCILYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="95"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8080577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="485" mass="55976" checksum="087ADE58D4A60537" modified="2022-10-12" version="1">MSLCFKIILSWAVQKKIMINIQSKDTQSPKYKIYFKLSTKIYWDLKKQKEGMILQSFVFPYQNKFKTLYADQLDLLRFKDLLDLRVTYEGIGDIYIPLLQIGKGSSAKVYSAQNVINSKVYAIKAIEKSFLNQTDKGSGLEAYNMEVSILKILSSYSSNFLILKEIYEGDHTYYLVTEYLEGQSLSEEIERAKGLSDKRLPIQNIKIIIAKLLSSVALLHSHKIIHRDLKPDNLMFAKRNDYYTLVLVDFGLATIETLDKYLFPKCGTPGYVAPEILTTNVGNKYTTKVDIFSCGCILYKLLTGKSIFNGKTFDEVMRANKKCEIDFNLPIDQNYITEDSIVFIQSYFNENLLKQLLKKDPKLRISARKALQHPFFDQISEYSTQLQASLLSTMKQNNLNFQIQCEEILSQYNLEEDIINSEILDQELNQIQIPKLKKCNQLIELQSPQKAMFQFFQKEPLSIIRSSRLVDLASPRGDFMDGIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKL1</accession>
    <name>A0A8S1MKL1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AtPDCT1/2 transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0630086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKL1"/>
    <dbReference type="OMA" id="YIYAHYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056361">
        <property type="entry name" value="AtPDCT1_2_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055311">
        <property type="entry name" value="PDCT1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34674">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34674:SF1">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24788">
        <property type="entry name" value="AtPDCT1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="AtPDCT1/2 transmembrane" evidence="2">
        <location>
            <begin position="74"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8080089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="277" mass="32700" checksum="3D86D86A2A664E45" modified="2022-10-12" version="1">MHISNKQQQDLSIYHGCEVQKLESSLKFDHRQSAVPDNPCYNFRFRIFIFFSYIVITQIGEILGTPQDDIKGIFDIGHNLTDGINQLYHSHTWFASLMQVISGLILDFAFFYVSLYWVFYVRNFRLFAALIIFYGIRAIHLNIFKLQFANNYYWQDPGVPTFVVKYGNYSDFFYSGHVGFLVICALEMRKLGKKWFALFFFISSFFQAFIVISFRIHYIIDVPAGYIYAHYFYNLVCYWEVKIDHILYWISTKCSSVKISQSSTVQTKIPDLDTEKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNG1</accession>
    <name>A0A8S1MNG1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATPase AAA-type core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0670014</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNG1"/>
    <dbReference type="OMA" id="MFISDYS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF5">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-type core" evidence="2">
        <location>
            <begin position="10"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8082008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="132" mass="14626" checksum="5B7AAF2F36062DAA" modified="2022-10-12" version="1">MGNLLNIIQVGSPGIGKTSSVNCLARQILGDSIKESVLELNASDDRGIETVREQIKGFAQKKVNLQEGQHKIVIQNKLTEGAQKALRMIISDQSTSTRFVLSCNDPTKLIDAIKSRCCILRFNRLSENEIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQH0</accession>
    <name>A0A8S1MQH0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0570236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8077144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQH0"/>
    <dbReference type="OMA" id="RLKHMAS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="12207" checksum="BF561F7701C3C0C1" modified="2022-10-12" version="1">MGCNVQKSKSQSNKLCQAPQEILESARIANEQDGLTHGQKSDIKESENFVSQQQQVQIGRRLKHMASSDELNKFINKKHSNPQSHFGYIKNSPEQRRKQSLMNSPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQV6</accession>
    <name>A0A8S1MQV6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0580021</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQV6"/>
    <dbReference type="OMA" id="QYNLNFE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="685"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="386"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="458"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="386"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="416"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="458"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="531"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="573"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8077254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="788" mass="93258" checksum="5FEDCE1F90068908" modified="2022-10-12" version="1">MEGQCECKNEDNPDIEYIGVCQNEECKEELFCEKCKGLHADHEDDTLVGEEFNQFIEEFCSPEYQMRLEKEQILNEFIIYYDNIQKENIEEGKMLNDVQKYFKEQNFLEIVKNLDKIKKYKEDLDNNKAAEIRKKLNEYKRLIKELDNKYSSEDNQVREQKLNYLQKGKELFHAQLYSESLENLEQALKIDNDCVDAILLKSQTLIQLENFDFAITACDQIIKKNKLNIEAYFIKIIALLLSQKYDKVYEELKKANHNCSYNFYCNIINLIKQLEICSNKVKQTLSKLNYDLKERLQSLILFQEMPAQMIKQYFTQLIQIIQLADQDEINHALFQYNLNFEIFEEDQIQYFVDLRPKLDNEAWQQMIKFLESLKEQQNINNQVEVPLNPNIPEQQNNEENQIQEQQNQLIQEKVPTDNTSQSQELGNNNQIQTSNNKINNQTPNQLIEDLNNFLTDPQTNEQQQQAKSQAPANQLNNQTPDQFKQDFNNFLTDPQTNEQQQLAKSQAPTNQLNNQTPDQFKQDFDNFLTDQNQNEIPSNQQQQQAQNQAPNQPKNDQTPNQLAQDLDNFLNDTQPNNITQNQQSIQSQQQQLSKETPIPIIQQQLADFYDQPQKQIIQPQQQVQKQPIIANEITPYSQQDLQNFMNEQYQEIQPNKKMIILDEDKRYSQILQYCDQMIIKSKDKAQFYFKKAMTLIKLQDYDDAIYYLDQASTLKPEEASYYYQKALLFQMLDQNSLALLNFDEAIQKDPSKALYYQEKGNALQKQNRQNEAEKYFELARQKQNEDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQV9</accession>
    <name>A0A8S1MQV9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0660142</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQV9"/>
    <dbReference type="OMA" id="KFEDHFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000053">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431:SF14">
        <property type="entry name" value="LD15323P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431">
        <property type="entry name" value="SORTING NEXIN 17 AND 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="163"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8081732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="320" mass="37890" checksum="24EA00D9431B2D3D" modified="2022-10-12" version="1">MKQSAQRYSVKIPKHETIDKKVYYTISICNLEGGEPKELKKRYSELESMHQKILEWIQIFKIKIPVIHFPKKKFLFHTNQSEESIIKRRGELQQYLNEILACPELHCLGVIEEMLPKEPKLSAQKQNDTPVDRRWQEIQQLKESYLAKHGDSIFSLPQKKIEQQSKQQYIFKFEDHLIFDDSALYTIQATDTITNKSWRFTQRFQDLRDYHRQIKNIHLDFPLPNFPEKKLVNISDAADLRDRKSQLEDYLNRIFKYQTIVESDIMVFFIAKSQLDGNEIGCRSKGTSQTTLENSSIKSKSQTTIEQFDDDHKQPRKITC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQY8</accession>
    <name>A0A8S1MQY8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-lactamase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0670060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQY8"/>
    <dbReference type="OMA" id="IICANCV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050789">
        <property type="entry name" value="Diverse_Enzym_Activities"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43283:SF3">
        <property type="entry name" value="BETA-LACTAMASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G07500)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43283">
        <property type="entry name" value="BETA-LACTAMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035938496" description="Beta-lactamase-related domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase-related" evidence="2">
        <location>
            <begin position="59"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8082059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="378" mass="42295" checksum="E21AB0F4231C7920" modified="2022-10-12" version="1">MKQNNFLPSLITVLLIICANCVTKDDLISQLTENLDIMDNKMSILIQGADGSIYLKEHLSSEGTQPYDDDTTIHIASGCKWIAMATIMKLVELQQLSLDTKVTDIYPEFQNTNPTMTLKNLMTHTSGYELADEWIFDLNISLQESVLGIAKGGQYPASKVYFPGGTKVGYSDIGMQIAGGMAEKISGKTFHELFDEYIAIPMGMKNAKFTAFDGETGNNIAIADGLLIRMIDYGNFLRMLLNEGYFDGKNILLASTVQTMLQDHTGDLPVSDEVMEEVVGEVEDSYSFAIGAWVYERDSDDKPSIYSSPGIHGFENWIDVENNYLCILYIRTDTEKEPEVTDLTEAIRPDILEFYLDESGNYSNLIYLGILFVFVIMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MS58</accession>
    <name>A0A8S1MS58_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0620104</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome c family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MS58"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000049">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002327">
        <property type="entry name" value="Cyt_c_1A/1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11961">
        <property type="entry name" value="CYTOCHROME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome c" evidence="7">
        <location>
            <begin position="42"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8079595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="154" mass="18132" checksum="850501F1A04032C4" modified="2022-10-12" version="1">MRSRKVYDGDVGFEMRGQSSQKFRRKGQVKNENEIEIEVPNGDLTKGRIFFSHNCAGCHDLDQDLNLGPSLRTVYLRRNGSKRFSNYGKDLTAYKFYWTRKKLWEFLENPEKMFPDTNMQLDGVKDPFLLASVIDYLQYLRVFTCDIRGQKQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSP9</accession>
    <name>A0A8S1MSP9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0670201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSP9"/>
    <dbReference type="OMA" id="FRFKAKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000051">
        <property type="entry name" value="Protein phosphatase 2C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="129"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="410" mass="46815" checksum="26469F89598090F3" modified="2022-10-12" version="1">MKLILFKNIQLKQMNQYNHTKVKQQKKQVHLILTPLKLEQSLRVKNNYLENSSYYQRTSPRTILPQFTPQKKKTQISFQEQKNSPQSSLVTSFKVRPRNFHQEKVQFQSNLIKIQQRLIPDDSFRFKAKYAARSRKGVQLGNTSKVNQDTYICCAKIENNECIHLFAICDGHGEYGHLVSGLIKTQIPILISKNKLLLEKNSSQGLMIIIQGLSDILQQSHIDMSFSGSTLVIVYIQNNKIYCANLGDSRAILLNRDDNWKMKPLSRDHKPNCKDEADRILANGGRIDPLMNGLGLFVGPLRVWTNQNVPGLAMTRSLGDEIAHSVGVSDKPEILQFDLERNDKVIILGSDGLFEFLSDDQIINCISPYYDTSNIEGACNQLMLTACNSWMQKCNNLIDDITFIILFLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTL9</accession>
    <name>A0A8S1MTL9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0630100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTL9"/>
    <dbReference type="OMA" id="QSYIHLE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="82" mass="9486" checksum="A0D771BC107D8844" modified="2022-10-12" version="1">MEQKHLMRYYVEEQQALHRAAAVYGSGLAMQLNLERNILGAPGQTSYIHLEIAMNKLNDIDELDFMGNKKLEQEYVKGFQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTX4</accession>
    <name>A0A8S1MTX4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0720088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTX4"/>
    <dbReference type="OMA" id="ETPNCFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8084457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="113" mass="13345" checksum="D2790CA35960E85D" modified="2022-10-12" version="1">MCDHRKRQLTYDDLDNSNMIPRAPLIKPLKDSSILIPELQLTYESPNNFKQIPLTIISEDERLEGFQLPLVGKHIKKRQRFQSENVRHTVINMMERQLLSRKSSKIQIESNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MUQ4</accession>
    <name>A0A8S1MUQ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major vault protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0640011</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MUQ4"/>
    <dbReference type="OMA" id="PKRPNHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08825">
        <property type="entry name" value="MVP_shoulder"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.550:FF:000008">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.570:FF:000002">
        <property type="entry name" value="Major vault protein-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.550:FF:000001">
        <property type="entry name" value="major vault protein-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.570:FF:000001">
        <property type="entry name" value="major vault protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000010">
        <property type="entry name" value="major vault protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.620:FF:000003">
        <property type="entry name" value="Major vault protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039059">
        <property type="entry name" value="MVP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041139">
        <property type="entry name" value="MVP_rep_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021870">
        <property type="entry name" value="MVP_shoulder"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041134">
        <property type="entry name" value="Vault_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040989">
        <property type="entry name" value="Vault_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041136">
        <property type="entry name" value="Vault_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002499">
        <property type="entry name" value="Vault_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14165">
        <property type="entry name" value="MAJOR VAULT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14165:SF3">
        <property type="entry name" value="MAJOR VAULT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11978">
        <property type="entry name" value="MVP_shoulder"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01505">
        <property type="entry name" value="Vault"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17794">
        <property type="entry name" value="Vault_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17795">
        <property type="entry name" value="Vault_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17796">
        <property type="entry name" value="Vault_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51224">
        <property type="entry name" value="MVP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Major vault protein repeat" evidence="5">
        <location>
            <begin position="48"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Major vault protein repeat" evidence="3">
        <location>
            <begin position="115"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="MVP" evidence="1">
        <location>
            <begin position="118"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Major vault protein repeat" evidence="3">
        <location>
            <begin position="167"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="MVP" evidence="1">
        <location>
            <begin position="170"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Major vault protein repeat" evidence="3">
        <location>
            <begin position="220"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="repeat" description="MVP" evidence="1">
        <location>
            <begin position="223"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="repeat" description="MVP" evidence="1">
        <location>
            <begin position="279"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Major vault protein repeat" evidence="3">
        <location>
            <begin position="327"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="repeat" description="MVP" evidence="1">
        <location>
            <begin position="330"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Major vault protein repeat" evidence="7">
        <location>
            <begin position="378"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Major vault protein repeat" evidence="6">
        <location>
            <begin position="463"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Major vault protein shoulder" evidence="4">
        <location>
            <begin position="525"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="684"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8080545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="848" mass="95857" checksum="628EB2B1EA83C706" modified="2022-10-12" version="1">MAEYVTRIPPNQYIHVLDQNTNTARLEVGPHTFIRKEHESIIEGPNKMISLPPKTYCKIQNPILRDKNNQPVKNAYGEVEIQRGEIEYRTADDYSNPFPLFPGEKLDGRVETLLIVQQNTALRLVAIRDFTVDGKQKQAGEEWQRNGPFTYYPRAEEQIVEIVKAEVIKPNSALKLKATQTFIDKNGKERRAGQEWLIRTTGSYLPDTFERVSEVVKGVVLTDKVCVHLRAQNEFTDVYGIKRKPGNEWIITNKQAQVHITDVYEQLVAVEQAITLGSRNYCIILNPYNPKLEQNDWGIQVLIKGETTFFLQPGEELLGGKIEDIVILGEEEALLVQAIYDHSEDDGQFRKAGQRWIVRGPREYIPGVKINVIEKRRAIPLDANEGIYVRDNRTGEVKEIKGKTYLLEAHESLWEKHLPENVEILVQRASTGQPYVPPSVQASGKMTYNFESEQLKPRDKTKIISFKAPHNSAIQLYDYKLKKSRVVFGPDLVMLGPDEQFTVIKLSGGKPKVENLIQTLTLSLGPDFMTDQLIVETSDHAKLKLTLSYNWHFKVNKDDPNDAQKLFGVKDFVGDACKSIASRVRGAVSSITFEDFHNNSSVKIREAVFGKEGNEIRTQHTFEANNLVITSVDIQGQEITDEATRRQLSQSINLAIEISSKSQEMQSRHQASKLEQEAKGQLGRQKLLDQAKAEETKIELLKQQAQSAKVRAEGEAVARAIAETMEKKIKAEAEVNQAELRSKALQIEQEALLEQQTQEQEAELNYQRQLIELEIKKAKELSDIEIKKFKQQIQAIGKETIIAMAKAGPETKAKLLGGLGLKGFLVTDGKNPINLFNTANGFIGENLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5D6</accession>
    <name>A0A8S1N5D6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0790037</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5D6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00065">
        <property type="entry name" value="FYVE_like_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="357"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8087640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="423" mass="49963" checksum="770BC3720758362B" modified="2022-10-12" version="1">MSCDICKKPFNIIWRRDTKCKRCNRIICTDCSKLQQVRDIFRNICKVCMQDCETIRKIISESLLAWNHNSQIAKKYWVKDVEIIKSVYKLNKEIKYTKIARDVKTVLQTPMNYSIQEFLNLLIFSNKDIETTITNVIDTYVTRNPQVGYNQNLIKLTIFLLCFCKDYVCLKILEDIYNKIPKQYYPEYQTTQEFIKDHDYMQHDAIQDVKFRFKLEQDQIIAAKQFIESAMMNYKNQFFLNLTFSCAFYIIDKLIQSTDSTDIDKFFSVIIQINIHQLQTKNSELVIARTTTVEGIKQYQNQMAKKRSSLYLSVDKCTQDSRNQSQLSCRTLQRKMDVNEFEADENENLSHQSPTLLQSQKQEITKLTQELLSLEQQINQLKIENQQLEEATVQNTAILFHNYENAISDLIDQTEILLKQSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N790</accession>
    <name>A0A8S1N790_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histone deacetylase</fullName>
            <ecNumber evidence="1">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0750079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N790"/>
    <dbReference type="OMA" id="GWLRAFH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09991">
        <property type="entry name" value="HDAC_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF44">
        <property type="entry name" value="HISTONE DEACETYLASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="2">
        <location>
            <begin position="26"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8085806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="481" mass="55419" checksum="2CADB6B2CEDF544D" modified="2022-10-12" version="1">MQNETNKRVAYFYHPQIGRYHYGKEHPMKPKRIAMAHNLIVNYGLYRNLDVYMNRQASLKEMEKFHEPEYLQYLSQFTSVRSQIVNEYQHYNQQPNITHFTSHIYNEFDLLDYNIPHTHEPIETNNNYPQPNSFRVGDSTDNPSFPGLYDFCQLSAGGSIDAAHVLISQDAEIAINYSGGLHHAKKREASGFCYVNDIVIAILELLRVHQRVLYVDIDVHHGDGVEEAFLLTNRVMTCSFHQYGDDFFPGSGDIDSYGEGLGRYHALNIPLKIGMNDETFTEIFQKIITQVMDIYRPEAVVLQCGADSLCHDRLGGYNLSTKGHGACVEFMIKYNVPILVLGGGGYTIQNVARCWAYETGLCLNKKIDAPIPTSEIYYEYYGPDYKLHFPIKQNVENKNKKEELQRIVEKIYGYLKSIEPAPGICFHDLPQSFYPEMNVEEEINPDQRYEQTLNQFEGAHYEEGNEILVDPSNGFSIHRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAL5</accession>
    <name>A0A8S1NAL5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0830036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NAL5"/>
    <dbReference type="OMA" id="RISQMIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="351"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="634"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="424"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="374"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="634"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="645"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8089172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="683" mass="79813" checksum="341C89443AB87CB4" modified="2022-10-12" version="1">MFDSQNSTSAKLTEQIKSQQSYKIREFEWFEVETKIRLFVQKLMEPTLKQMHEDRMIVDELQQNFKTNNKEVSYLRDIVLNQGAGIPIFEEFKQRFLDLEMKLDQQKRESIDQFGNFRQSLDSTDNEISKFLQSVRTLEYFKIKLEQDLQDIQIKNDNFKSSVESIIKQLSSDLTESTKNWKLTAIEIEQQFGKVDNRHSDLINIVKQEQVKLKQISDSILEINKKQNQNINTFEVIDTRLIKADVQITTLKKQILEVDQLINRNKQVQMFLIYYLPMYMQAQISDSLFTCLPLKFFYKFTQFEKQKFDYLDKQLLEYNGDPNIQILIDEHSKTLAEVMKRNSRISQMIFMNQKKEQKDSHSSESDMDSDNRSQRTNVRNSVQIRQDSTNNHRKESIYSKDMIQQSAIMQQQKKNSGQSNNSNLIFYEDQIKQIKDQFQQEIDELKQFNITLKKQIAQNQKETNDQISINISSCQSNNQKLLNTTTNQIKNLEENIKKALSQENSNSNDLIFDQVKQFKKSLQTILHFLVLITHLLIQDEIDRQGIQLMGAKDTQIDGGLVYTGQSKKGPSIQLNGECLSCSGQSIQLLQCFKLACLAYHPSDILVDGKSFKRDQLLEMCTTLLRQAKQEFLDQDQNISQPNTQPIASRQRSSSIPISPGRLNSNFKFNKTRKLAPMTNIQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NER9</accession>
    <name>A0A8S1NER9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0740012</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NER9"/>
    <dbReference type="OMA" id="IMATFID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8085194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="478" mass="55449" checksum="89471B862E1B652A" modified="2022-10-12" version="1">MNDQKKDTNSIKDDKIQISFKLIPESLANDIKQELKQYVETLAQQDADLEKIEEYVSKINSDDIISQYYGLNRCRKLLSDTIVAQPIESLIQKVLQTNIQYNLFSIVKLNSIPLLKYEALWIISNIVFGTQEQIQIILDNDGINILFLALESQYDELIELGIWSLANIAADNIKFRDMLLQQGILFHLKRIWKQYKNTKSELIKTIVWALSNLAKGKPTTKFRIRELIQILSEIIISTNDEEQLIDSSWSLSYLIQNIYEIDMQIDKRLIKKLKLLLNSTKHTLIIPALKNIGNILSGNDEQTNQVLQAGVLQSFEILLQHKRKSIRREVYWSLSNIVAGTIPQIKQIIRNDQLLKSLFKQLKLSDLQITKYIAFFVSNSAIYAELIDLEYLIVNYEFIQKLSWLLDKNDEQIIQATLEGICKLIQKLQFEGKFAMYKQLIEDQKIIAKVIQLQSHPNETIYENALKILDNLVTQESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHG7</accession>
    <name>A0A8S1NHG7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cathepsin L</fullName>
            <ecNumber evidence="10">3.4.22.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0810028</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in extracellular digestion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.</text>
            <dbReference type="EC" id="3.4.22.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.15" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NHG7"/>
    <dbReference type="OMA" id="CGQNINH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000104">
        <property type="entry name" value="Cathepsin L 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035942015" description="cathepsin L" evidence="12">
        <location>
            <begin position="25"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="30"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="114"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8088425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="309" mass="35729" checksum="9012E3C7C3FDBDE0" modified="2022-10-12" version="1">MIRSILTTAAISLLLLTNYYQTPSQTINEFDIWQLKHQKIYIGEERIYREAVYQANKQKIENHNKDETRTYDMGENQFMALTQDEFISLYLTQVDAPKLEQQEQIEEIFDTQEPLKFVDWSSYTTIKSQGQCGSGWAFSVSNSIEAWYGIRGGRNITASAQQLIDCDVYSKGCYQGTNYNAMHYAQQVGLMNSMSYPYIGQQQGCRYNRGDYRINNYQFVGREQQTLQHYIQNYPVSVGVDAQNWQFYNNGTFSDCGQNINHYVLAVGFDFGYNWILQNSWGYGWGENGIIRLSPGNTCGILNQAYQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJE2</accession>
    <name>A0A8S1NJE2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0850199</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="LQFTNQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="520"/>
            <end position="1911"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1976"/>
            <end position="2185"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2349"/>
            <end position="2451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8090301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2564" mass="306253" checksum="1C09EE551A8079B7" modified="2022-10-12" version="1">MEDFSDDDINYFIAKLSEDCSKSFFDNYQNDSQLLGIKLDEQLIKQIRDKEEMMKTMFDLLNYIFNKQQKILADYEDLKIQCEHSLLVQKQEIQQTQYQIEQLQTQNAENQEKNQQFLDQIEELENQLSQKKSEIFQMKQNEGRISQNHQAHEIKSQEQKEQLQSLQTKVQQLEEINSKFKSEAIASFNLLRQYEMKQKDYEDKIEILELQLKNNQREKDKLSKQYEELFKKQQSEIMTSTQISINNNFIHNSPQKSTRKNSNYSSNNNNHETIQKLRRQSGFLNQPKLEQIQKQINNIPESESESENELTSGKTLKQEKNEKKNIQKELNNLKVKIEELEKQKGEMEKELTQNKEMISNQDTKIRQLETNQKKMNLKSTQLEKKVTNLTEEKNALEETYKQEKSQEQDQLKKQSEDFMNKIKFLQNENQQYASQLKELNEYKNKYHELCLKNLKEEDEMEAFLRTHESAATAHKQMQKQQEKQLYQINLNQILNISDADLIFTRARSNASVFKKKDIPIEQTEIFKKKLKEQLDQSLKNVLMSEQNNSDILQKTLLQVNQYELQLKNMQEENQKLKFEIDEQKKKIDLLNFKNKELEIQNDKDKLEMIEHSKLLDQSKVKLYKSEQQLNLLNEKLKGLQEIVDQQEKDISNLNEKIIQQSKLMDDQAKISKQEIDRLNDRNDNKQLKLDQALIEIQHLTTDNKMAKIKSDTDIKNLTEKNTKLQNQLDLIVKQKEQQEQEFNSINDINSKMEQTLQAIMKNYSDIEIKCANLEREKQQLEQQNQEINEKLKKIIQKKKILKELNVAKDQNYIKIKTELEIETEKCSNQQNKINSLKDDLENLKQENLIMYNNIEQDRNQKIEESNQLKTVITELEMNKTELNRQKEQLDKEIEEIKLQFTNQNEGNQQKIHFLEEETQKKDKESEKLRLELEVIKKEKEHQVASLKEELSESEKKLLHIQNELQSLQEQFKQLQLSMNASQSEAHNSYQELQLKYNSIEQDRNQKIEESNQLKTVITELEMNKTELNRQKEQLDKEIEEIKLQFTNQNEGNQQKIHFLEEETQKKDKESEKLRLELEVIKKEKEHQVASLKEELSESEKKLLHIQNELQSLQEQFKQLQLSMNASQSEAHNSYQELQLKYNSIEQDRNQKIEESNQLKTVITELEMNKTELNRQKEQLDKEIEEIKLQFTNQNEGNQQKIHFLEEETQKKDKESEKLRLELEVIKKEKEHQVASLKEELSESEKKLLHIQNELQSLQEQFKQLQLSMNASQSEAHNSYQELQLKYNSIEQDRNQKIEESNQLKTVITELEMNKTELNRQKEQLDKEIEEIKLQFTNQNEGNQQKIHFLEEETQKKDKESEKLRLELEVIKKEKEHQVASLKEELSESEKKLLHIQNELQSLQEQFKQLQLSMNASQSEAHNSYQELQLKYNSIEQDRNQKIEESNQLKTVITELEMNKTELNRQKEQLDKEIEEIKLQFTNQNEGNQQKIHFLEEETQKKDKESEKLRLELEVIKKEKEHQVASLKEELSESEKKLLHIQNELQSLQEQFKQLQLSMNASQSEAHNSYQELQLKYNSIEQDRNQKIEESNQLKTVITELEMNKTELNRQKEQLDKEIEEIKLQFTNQNEGNQQKIHFLEEETQKKDKESEKLRLELEVIKKEKEHQVASLKEELSESEKKLLHIQNELQSLQEQFKQLQLSMNASQSEAHNSYQELQLKYNSIEQDRNQKIEESNQLKTVITELEMNKTELNRQKEQLDKEIEEIKLQFTNQNEGNQQKIHFLEEETQKKDKESEKLRLELEVIKKEKEHQVASLKEELSESEKKLLHIQNELQSLQEQFKQLQLSMNASQSEAHILYQQLLKEQHTKETIHQNELNQLNQQIIEFNLKIQGYQTEVQTLNSNIQQLTISNNSQNHQFTQYYIQDQLQILVLESIIHNISLEANAYKQKSEILLKESQQKIIEVNLNLQQQLTNCQNFQQLNNEIDQELKRIKKISQEDITMIQQLTHQLTLKDQQINQIQIQYQQFEQQQNKSLQDLEDLKILFEQIQKEKLQLEIHLAEQYQLVKEAHTQQDLYSQELTESKLEVQRLNDQISQIFEKDKNQSVETQNLKQLIDQLSEEKQYLQELTKTFEETIQRKDEEIDEINKNLEELKQSLTLKVYEIEQLEQDFKQAKEDLILNQQKLLDEQNFRATQETQFKIQLENYIKSQQKNSDVLDISENKIELLEQLQGQKIEFDVNIQTQNEKLRIYELQVLELRNEQFKYLEKINELSEIINSESVFREMQKNFIQEILVHLIKQSDGLKKINQQETENLIQDDYDDLDKLKTSTMMQINILNRQIELTINNFKEVQMKILSSNEQINQLEQSLKESQENQTNNKLLIESLILNISGLNEKIEGYQKSSSVNLHEITKQQEQEMELLKKDYQNKINRIQEALNRREQDFNALNDQYNLMLQDQINKTEELNNLRKKYDDFMNDIDKMTPTRSPSNSISQKPNQIDQYQQYFILTAQAVALSSDDPMAAAEIKLKNFYQEVKNRNLPFHQWHIWLQLKLKEELSKNRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJJ6</accession>
    <name>A0A8S1NJJ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0770110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJJ6"/>
    <dbReference type="OMA" id="FCNREND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000085">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="178"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8086814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="461" mass="52245" checksum="6886711E38B39204" modified="2022-10-12" version="1">MKQKVSQISPDKLYASLLKRKNEQEQQARLKSPIVTTNKYLVSSNRNGGGTSKIEAKKSRPASGKDLKDVSSVLLKKTHARQNSKLIETKENSQNLSTSALSLLLKIKQDKCENKQSQIQQPKDLYNQQTIQKKYQKLMEMTKQQQQLILKPKLFKGPRSISNPDISTMQLQIVKANGQSAAGMLYNGQTKTNQDIYKLIQRFCNRENDWYIQVSDGHGTNGHQVAQFLREVLPQFVEQGVVHLTSCYERDKQINLILKNCFLQTNDELMDSGIDITYSGATTVVVLSFDNVLYCANIGDSRAIIGRFDNKLSVIELSKDHKPDCFLEQARILQRGGRVQAYSDEEGNPIGPARVWKQDEDVPGLAMSRSFGDYIASQVGVIQEPEIIKHSLLPCDKFIVVASDGIWEFLSNEQVIETVYEYYKRDDSQGACQKLVQLARDAWQREDEVIDDITIVVAFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJK7</accession>
    <name>A0A8S1NJK7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0850229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJK7"/>
    <dbReference type="OMA" id="WIAVNEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8090361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="276" mass="32645" checksum="7B8C3F9909D3ACA4" modified="2022-10-12" version="1">MGTCQSQKQNKNFANQINQINLATQFPRLTDQIHFFDQISLEQLNQPDLSTFLSKHINKNRIVNEGKTWIAVNEVKCILNTKSIVLIRNFIPETDAAMQLIYLLKQLISVSVRVQELQVDFNEYAVELFHYFQAQLVQVSIEVLLAVNTLMKKQEHWWNDNYFSWRKNYIQSMFKIKQPDVEKVEFIHPFFEYIALLKECAKYFCNQLKSPDLIAIDIKAQQEVLEQFYNQLRTGFLQNQSTQADSINWEHHLASKQLEQRLQTYTLFIKKKNSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJS7</accession>
    <name>A0A8S1NJS7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0920034</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8092928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJS7"/>
    <dbReference type="OMA" id="QSKYNDE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18605">
        <property type="entry name" value="ABC_6TM_MRP7_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF330">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="510"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="615"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="108"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="387"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="699"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8092928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="908" mass="105187" checksum="0A7DF1D02F5B32C1" modified="2022-10-12" version="1">MKAIKDFISNPKSIKFLKWENKWESKIMNIREQEFAIIAGSKWLDSLCVLFWSITNTLISSVTLYVYSQNNDLENVFTIIYVFSLLTNPLNSLPWTLAGMLQAKTSFLRINSFVITRKSNNQFKEGIEINQAELKWDFDSEFTLRIPHFKIDNIVFVGGKVGSGKSSFLLSLLNQMKIQSGYFQVNQQFSYVSQQSWLFKGTIRDNILMQSKYNDELYKQCLIQSNLIDDIQKYPEKDLFDVGPDGSKLSGGQKQRLCICRALYNQNDIYLMDDIFSSLDPQVGDSIFNNLLKITKPIIFALNDNYLQRYKKYADQIIIIKNGQVIYDKQEIEMYFQDLQEQTFDLKEQKVNLVQQEIIQHERENQQDVEIKKTQKSLSFYLKSLGLLNLLWILVSLALMQISFNVYDIWLNTYINENPMFIFNNSFVFTLFMLVLIDIIIKLSRAVSFAYGNLEQAKKIFVQLLNHVIHAKCSFFDAEESGEILIRFSDDQNVVDNRFPFELNLLCNNIFNFIGTFIVLVVLQYYLILPVIVSALVFYRLQQLYRPTSLSLRRLDAETNSNLATSIFEQFKGILTINAFNKREEFRNIFLSKLIQSTQVQWCSRVIALWLQIRLQLVAGFIVLFILLFTALSLFYELPISKSTLQLLLYYSLTKISLFRDLTLSLTTCEQELVSYERQNKYCNNNIEQDKCLNYSEDIKFKDIKLQYFNGQLALKGINLEIEQGKRVAFCGRTGSGKSSILAVLFQLYEQTDGDMSVPSQQLFEWRNKVGVIPQLGFVFDGTLSENLDCLDQNLISKYVAMDGNSQIDSNSISQGMRQLISFSRVVLQNPDIICLDEATASVDQQVEDFIQQYLRECGKTIITISHRLEALKDYDRIYYLENGLVVEQGTFQELVQLGGGFANLRNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJU8</accession>
    <name>A0A8S1NJU8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0920044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8092948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJU8"/>
    <dbReference type="OMA" id="CAIKPID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="321" mass="37612" checksum="BE6102807172D019" modified="2022-10-12" version="1">MKTKYNKLSPLDESKSAKCELLNEDSLKMNILKQKIIHERSSVLQRDGELRKISEELHNRIMNQRLNFNKPLKYTQFVIHQRKNDIKKNSNAVLNIVKKEQQLQQELSKRDISQFASYEQEQDKSQLIDKMPINKKKLKMKYRDIVKSILIKSIAQSFHDDSINVINYHTKPKSISINEQPILPPSNNCAIKPIDQYILTKLENIAQDQLDNSYMPPLSQNEIKTSTQKNSLLKLIPITTATAESSKLKRNRCQDDFSNSKLIQEFKNESGIKNYEITRKAQERITLYQKILKMQNESRSQSQSCYIKKKIVNPIQNRYNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NL89</accession>
    <name>A0A8S1NL89_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0940194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8094057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NL89"/>
    <dbReference type="OMA" id="YHIIASV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="275" mass="31114" checksum="051750D378859CF2" modified="2022-10-12" version="1">MFKGKRVHSQLIDISLHGTLFKFVKHPKLNAFISIGNDHDLSVWDLESLNLMYKKEGNEYAIADALIHNDYHIIASVNGTLKIYKGDEEPKVTQLPEIPLSICVHKDQLYVGTTSEKLLIYDLDTVELKSIQNKQPWVTQLRSHDDTLYIMGNKISFNDNTVDLGHKGQIMHHSILGNKILTGDSSGKAILSTFPEFTKLYEYQMESAVVQLAIIENKEQLSLIIGTLDPGIVIITNGEITKIEDRAAPFIICSQNRIINRSNILTRQAYCDVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NMR1</accession>
    <name>A0A8S1NMR1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0880127</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NMR1"/>
    <dbReference type="OMA" id="YALMLVN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="120"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8091551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="356" mass="41335" checksum="BCC8E94A9F151667" modified="2022-10-12" version="1">MKSKQSSSSVASESVSDFTSGVSIRSGKVLTVTKLEELASAIEQKSSESENIKEVENPEEMLSPLDFKLDLSMIIQKNVKTEGFYNLVLQNYLGKQQKPFEFLNKDDKGFDDEERIRFLNWLIEITHAYQMNCSTFFIACSILDEFQKKTQNITRYNLHLTGISCIYLASKFNDVKPLKIDQAIDISHKRLGRSKIFKKELEIFECLDHQLNFSNGFLFLTLLLRLYLSKIKDKENDIIEIAYFTLKLCCFDYELQSKYYQSEISAGCLSYAVMLVNSNNQGRVNEHITTQLLYLLKNSNVVKDQQIVIVGEHIEGLIERYFTDIQFRGKIQKLIEYNLIQTDLLKFYLDNRNQDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NNT5</accession>
    <name>A0A8S1NNT5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ELMO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0890180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8092026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NNT5"/>
    <dbReference type="OMA" id="INFIEAW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006816">
        <property type="entry name" value="ELMO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050868">
        <property type="entry name" value="ELMO_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771:SF2">
        <property type="entry name" value="ELMO DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771">
        <property type="entry name" value="ENGULFMENT AND CELL MOTILITY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04727">
        <property type="entry name" value="ELMO_CED12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51335">
        <property type="entry name" value="ELMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELMO" evidence="2">
        <location>
            <begin position="236"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8092026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="405" mass="47280" checksum="1ACE681D6F2E4607" modified="2022-10-12" version="1">MIANQKAMQKKRNIQNIIDNDDEMGEVAEWAGISAAQAKDDDDFDFDNKQVFQQSNDHVEQARQKINQKLNKKGTNVNQRNGPLVNNLKDKAKSIALQNNQNNETPIKQQCQSSDDENEKPRIDKGEESPDKKVFRLSERSIGKIEEEFEQANKERMMDLEKKKNEIKQINFIEAWDQLKGSQDIRDQIEFIQKKTSFLDKLFGCFSIHLKSSRLIQEKNQVCLMSQLSFDDNNESHFRILYTIYCQLMTTDYCLRYGSHWEMIGFQGTDPATDLRGAGILGLLQMLAFISEYKIYIKQALKIFQDIKIPFSITLINITTFVLVSLKDNKLNQLINQEDSVISVINKLYFAGFHLLTKILKKEQITFHTIGNHLTHIRKLIHEQPQKLIREFHQDIQRFYKINNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NP85</accession>
    <name>A0A8S1NP85_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0950094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NP85"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="21772" checksum="B6F0F6F6C140F2DD" modified="2022-10-12" version="1">MLTYSCLVQSYMIQTNSLSNWFLNLYKTWMINIVSQHQIVLKIDLETELIELGNMVFRFPFQCTIQNVNQNNSILNKEFQIKNCQMLWVNQILLFIIQAFYLTIQKKKRCLFILKQTQALLTILNLDGTLKIGVSQPNIQLKKENTSIFIQILMRVHKYKCKVEQKQNFKQSSLDPNQIIFIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQM0</accession>
    <name>A0A8S1NQM0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0960047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="RTIMEKD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="520"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="679"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="827"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="981"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1124"/>
            <end position="1559"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1604"/>
            <end position="1709"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1763"/>
            <end position="2450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2473" mass="295328" checksum="61C7D22E8B22FD1C" modified="2022-10-12" version="1">MSYTPQQQEEIRAVYQRNQQLEMQVKASLNEVQQLNQRLQMQEQAFKQSQQSLNQQLQKLSQDLSIKTQQLVNSEQQFKRQLDHTNEQLNIVKQQSQLRIDQLSQELKLKTTAFDKASNELQAAKHEILLLQKNAENLNVVKSQQLVQQDANAKRVIDDLNDKLRLISVQSQKQIEQLQQELAIKKQEIDRLNQDLVSKKNEIYQQQQKIEATNIAKNNQISQLESLSKKQLQEYNDKLNQFQSQAQMRIDQLNQDISNKTAMLEKANQEIAQKKQEIQNNNKHFEQVSNQYQQKIQEMMGRLNELQLQAEKEQNQFGRTKEDFQVKVQNLSRDLSIQQQQSNQFAQAMQKENQQLKQEIINLQQQIAQRHQQYSEQKSQADAQINKLNIEISKLKTDLAVKEQQLESIEQQFNQYKNTTTKQIDSMIQQFEQRQSEIHQDNEIQSNRIISQNKQILEKTVEEHKKQIEQLDKFWNDKYKQLSEQGGQDQQRITQQYLTIIAENERKFQNLFKNQEQSYLSQLEAQEQHFEMQINQLSNQLKQISGDKESLHTQLITAQAQLRDFDQKVRELHYHGQTKSEQAYLQINGLQSSKEELFAFIKEKQIEYDSKVFELQQQMQSLASNKEAVEKELSLERVVKDGLKKEILQITNQQQMKDMEIRNEISLKNSEIIKLNKEVERLDGLFKQATLELKRINEQNEDLKLIDDFQLKEKDLEISKLKQIEIELKNRIQQLQKQLSNQEWQLQQENIKLKQQVKDLNNDLSILNTRINMLKTQNKDESERYQELLQSHDIELQSWADRYKDQSQQIETMAQQLFAAQQQNKYKEQLIFEYDKLKQEFDQLNRQYQLLQENSLRNSNIKENAKNVEIQQLQEHYEQQVIQFQKEIDFMRDNKINQRQDTSVIDFSVQITALNDQLVKKNNEITNIRMQANRTIMEKDNQIILLQEQLEQLISKVDIIEYNQNDQAEAIEKAENLELILNEKDKFIVQLKEELKNCKDQILELEEQLKIRQNSLDMIQQQASLISELKSQVEKRQVENQYSVQPKPVQQCFTKSIRSSTHRMSGKKSQTNEESQKEIISYVQKVTDVSFKQQSNQQIISIVNPQISETRQILRQSQEEVQQRNLLNQSIMVLQQELKQVKLNLSNEQQVSSQLKFELSQANAKIEQLLVEQSQLKNKQQQNDNQNIDQLNTMNMKTYESNIQSLKSENQDLQQKSVEQELQLNKFSQRINELQNEIKKQKEQIQQLNDSENQKENKIFELQSIVDRLQNNLKSEQSIVRQKQSEVKQITYQQEQIQQMLQQEYQEIKKNNNELSQQKLKLELEIQELKSQLQQSQVTQQLYESKITKLNTEIIQVREKLVQFEQIQIQIEQNKTQINQNSEQQQYQQQIQELINKLEKQQQQNNLKQQQLNNQLQILNEQYQDAIFKFENSQEQIKQLQNSLTQQKQQYLNDMNENSQILSIRNQENSNLKIQIDHLTQQLNAYKESNTNVQKWQTQVDILQKQLREEKLQQQDLNNQLQNQLFQFQQSKREWQDLIQRLQEDNNQLQTNLNRVLTARSDKEQIDNYSHQQNQNIQITTNTIKTQFMSNTQGLSSEKILIELQIAQEENVKLISQLQVLQLNNKQLNKEIQDFDEKQKQLENRYKDQYLRAQLEHDLSFKKIEQENVKLISQVEVYQKKQQQQEILIDDYIAQIEKLNIINNQLQSQLLLVQKDNSNFYKDQDKQIYKLSEINQQLLTQITQLNNQRNTDQLQIKDLMVIKQTLDEQLSKHSEEIISLNERLKDLSDQNTQLTHMNSEYLNQITGFQVQISELQNKLSQNIYEYKPRYSDQQPRQSTSNNSRQIEELNNKIISLNNQVNILNQQKSNIQQELFSVRKQLQNEQDDKLSQQSRQSRTNQYKILSEMEERIRQLQQQLEEERLGKSMNNGEIELKNRQIMILEQNNKKLQQQLEQLNLQIEEEKTLLYKEIDQLSEQNKRTKQQKNSEYDIEYRNSIKQKETIQQELIQANQQIKKQQSRITILEDQITKLTNGSIDMERQNRFIIELEDKLKINQRKYEIEIKELKNDKQSLNQQLENVQQELEEIKSKYHIEIEKTEQKLQKENQRFSLQLNDKAAYLSQLEVKNRQLKEEILNQQERYDEKLRQANERIKEVTEDLETMIDNYKKEKQLSQKWEKQNEDLRRQLQSQVDDIKIKTERLSVEKYERMSLDSRKQIEQIQQQLNNEINGLKQEIRQKNEQINQLKSTNQSLQQKLNKFETYIRDLEDQLQQFSNKEEQLLRELNQSKNQSLADSLNVKVIALENNLVKAQQREEQLIQKIRQLENQQNSNRNNSDYRNLEIKNNNMFEEIQILVQTIKSQDQEIANLKNELTSYQREVSFMKEVVKEDTSRLETSILDDKNRRIVELENKCALLANENTRLNQLKMKQVDEWKLKYDKLQLELEKSLNMKGWDDQSRNIVMSSNFGYLSNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUV5</accession>
    <name>A0A8S1NUV5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anoctamin transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0860233</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NUV5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF73">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="6">
        <location>
            <begin position="87"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8090834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="209" mass="24554" checksum="85EBCE147DA8FF96" modified="2022-10-12" version="1">MSAMLLCITIQEFYLINKETWKMHYKTVGRLLHQIQILLMSIQSKEYHVVQQLGILFLIQRKNKKQMCRKFYLNNKETMKWHYQYLYQNSFKGKHMSSIGSDQLNSQGMVHSQMIQRIVISASVLIFLIGSDIGIINGLYFFNLFLKTELDSAGNKFLSLEVINSGSLDNGIQQFIDFYYEQIAKILTDFENFQTSHQYETSFILKNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZ36</accession>
    <name>A0A8S1NZ36_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0970165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8095053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZ36"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8095053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="102" mass="11902" checksum="23D58580063A5888" modified="2022-10-12" version="1">MQFIQTGGGSYDQGQKNGLQLILSDGFYEYLFSNDFCSWSQAKKLVNGILITNIIMIKFGNKCNEIIFQYILGVVFMKMDQKMVDGRFDQQFQRQLLSHLQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5E9</accession>
    <name>A0A8S1P5E9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1060130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5E9"/>
    <dbReference type="OMA" id="HENMKQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8098247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="120" mass="14539" checksum="9F85F0BBD2FE2DE7" modified="2022-10-12" version="1">MDLQAINSNYKNFLEEIGSYYSVVDDQEVQILRKKQDDCYQNFLDVHYEYTKCISQIDDKYSSLNKTFKFKTEKAAKSYKSCLQNKKVEDCHERTWKHLHENMKQYISLLRRIDTQTIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P925</accession>
    <name>A0A8S1P925_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1100075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P925"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="172" mass="20436" checksum="069FF3424911C713" modified="2022-10-12" version="1">MSYYKPFYQSAYDRTAPVYPPSQYTPAYYGPYDRPHSYQSRAPMRGEQWSEYIPVEQRYTDYVPETKIEYRPVEKQYTDYIEVKHETDYVPVPRLEKRVEYIPVDRYDEHVDYVPVQNSHVVRGPQSRVGYGSQSQYLPPPPPAPTSYSNYRYSPSRVSGYRPATGYGYRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBK6</accession>
    <name>A0A8S1PBK6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1110129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8100098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PBK6"/>
    <dbReference type="OMA" id="EMSTHQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="115" mass="13895" checksum="9D2C8CDFFAD17842" modified="2022-10-12" version="1">MSQKIQKSQLQILSKSKQIKVKEDCDEVNIFNSYISMHTEERDNHHKEFKDKEYQQRKESERNCQSVKKIEMSTHQQRKRQSQSAHQTSAVKVQFQTPLQKKTHRKFYKTPFPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PDT7</accession>
    <name>A0A8S1PDT7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1140116</name>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1140117</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8101114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8101116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PDT7"/>
    <dbReference type="OMA" id="WAGVEHD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045862">
        <property type="term" value="P:positive regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="70"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="105"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="8">
        <location>
            <begin position="254"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8101114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8101116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="341" mass="39768" checksum="B83DE19B8D201AB1" modified="2022-10-12" version="1">MFPFVSNIFRSDLPLFIFDDIFVPGPGIRVGVPRPPYTNQQFRDQVPKPPNLQKMQSVKKSLADYSETQGQYWCILGDMSFEKGDYTKAIQYYDRAIELTDSTESSFFRSRGLAYKKIGNLEQAYKDAIMAIELDDRNIKAHLLCGQVLAERGKSQDNTHEIETAINRLTKARTLCAGQKKQYYEDELSKYIYRAKKLLWYKNKELEDQKKRQAIQNYSNYLQQRIDLNEEQRKKEIDAFINSIGNPDQKQNYDIPSYLICKITFEIMENPVVTDAGHTYERDMLIEAIQKNGPVDPCTRQPISGEFYPNHNIKQATQDFLLNNPWAFEYQSGENYADIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGQ5</accession>
    <name>A0A8S1PGQ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1180029</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGQ5"/>
    <dbReference type="OMA" id="VINPMES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053328">
        <property type="entry name" value="Uro-adherence_factor_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34592:SF4">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_31, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34592">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="650"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="728"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="212"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="339"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="587"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="650"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="662"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="728"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="783"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="837"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="847"/>
            <end position="857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8102297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="877" mass="106323" checksum="74760F9C57B97D34" modified="2022-10-12" version="1">MHISNPISNFLVQSNHLIILFSIKQMCYHAHKVFEKINFKTIFRLGYLEILEELEILLICFIKNILIIKKSKRNMNAEERPGTSFTSKRQVQTEQRKRQQMNLGTLETEDKSTGMKIRPITQDQQRNSLQQKRQKMIPQAILYDRESLYEEVINAKQINNNLQRENQALHTKIKMMDEELQRMVRATPISEQVINPMESFYQDSKPMKQHLIMDLKKQLRRLQEESIKKDQQIQFLQKNTKAMKIKELEIELLSYQQEAQRLKALMGMKFNDSDNDLLRLRADYKLLEDELKQLKIKCKEYESKATMYYNEKLKKDKLNQYLKNQIESLKFKDPEYKKQNKITENEQQLKQDIDQKQVQIDTLISSLKAKDAQYAELDKRMKELEREQQQIIDQLERDRQQLREQCNSLKEEVSQQSGRRRTVQNNSFLENKLEILPEGPEIIDQKKKLLPCVTKDDVNLICKQIKFKLKAMKVPFEKIDNFIHGDVHSVIRIEELNEMLQAEPFLLNEVDSLKVARYLIEDNDERFVEFSLLAEGLIARAKSILKNLIGKYNLLEKNEEDQLFFEIAIVLSKYRNSLEDFILRSKKKQELCSIEDLEEALKFVEQTFNSTQYEFLLLKNYELTQELIHINYKKLFELFYVDQQVQQKTKSESDLPKEQVKKQSNNEIQNNKDIQQQQKQEELLQQQLKKQKEEDEQKKQLQLQQEQERQRQLELEKEEEEQRRLEQQWKEEEEERKRQEEEERIKQENEAKRQKLLEEMKKQEEQENLKKQQEQQRQLELLKQQKEEEERQRKLKEEEEERRLQEELLEKQRLEEEQNRLEQEYEQQAEYEKEQNEQEENFEEYNEQQLYQQQSERQISEAQNKSQKDYEDEDFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PK50</accession>
    <name>A0A8S1PK50_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1210090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dynein light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PK50"/>
    <dbReference type="OMA" id="MNDIMIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF35">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="1" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="88" mass="10746" checksum="5F7EF4FC67359711" modified="2022-10-12" version="1">MEQIKLQIKATDMDEDFIKKVTDITQSAMQQFRTEKQIAHHIKYEFDKFDQFGWNCIVGRNFGSHVIHQTKKYIFYQHKELYFLLWKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PL14</accession>
    <name>A0A8S1PL14_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1220104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PL14"/>
    <dbReference type="OMA" id="NKGQESV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="235" mass="28095" checksum="10A5F68CCFB8E7D5" modified="2022-10-12" version="1">MQLMQAKNDHQQESSNNIKHLRYKSSYLIRNRRKIYDDNDFREVVQNFYKLVSEINKGQESVQPLLVKKITKPPLRKQTQQSKQRNASKNKQPTKELTQSKRDVHFRPDQQAQQYFNSCFSQQIMSHLLPNFDQPYCKQVPFLYDKPKPHLNFKRNQIHIQTAYQIYIKEFGSGFVESQDPTSFARKVMNSNFNCKTQYQEEEKESDKSEKLNKSYEDGKKKPLKELVREFQSDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PPS0</accession>
    <name>A0A8S1PPS0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T1260023</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PPS0"/>
    <dbReference type="OMA" id="INNRTPG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14008">
        <property type="entry name" value="STKc_LKB1_CaMKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000206">
        <property type="entry name" value="Serine/threonine-protein kinase Ssp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF77">
        <property type="entry name" value="SERINE THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="161"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8105046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="474" mass="55074" checksum="172E18E011B50BED" modified="2022-10-12" version="1">MLQLPINNRTPGQNFSFNQFASAPNSPDRNTPNTIKDYIKYRQRNLIDFVKSRLQSRQNSPKNLFKVDLIQFAKKKYDSMPSPIMGENKFLSTQRSIGDSIQIVNGQGMTEDTLFDEIIDLENAINSNGFEKKVMGSQKQINHSIMKKEVTEDGQVKINQYTILQELGKGSFGKVKLAKDNNRKYAVKICDRKKLKLKLLSSKMDAYSLLDKEIAIMKKVDHENIVQLYEVIENPNNDKLYLVLEYMDGPQLLQIKNKNIDTIWRLFRDFMLGLEYLHNFANIAHMDIKPENLLLNQELRLKIADFGVSQIMDDDLVKSKIGTSAYQPPEVFTEDQVRGKPIDVWAAGLTLYQLVYNEHPFISLKQDQLKNNILTQNIKWRETSDLTDLLKRMLDKNPNNRITTQQVLEHPWVTKNGRFPLKNEYQGNFCVTENDIHNAITRLSFNMAIRTLNKLKQKLNQSRQRLKQRKAQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PU70</accession>
    <name>A0A8S1PU70_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1290120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PU70"/>
    <dbReference type="OMA" id="HTESKCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="4">
        <location>
            <begin position="96"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="159"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="195"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="231"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="337"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8106138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="440" mass="51500" checksum="55472CBA402DAD54" modified="2022-10-12" version="1">MQETEQSEKDMIGSLLDENESECSEFRSSITKSQQHSQSPIKSFKDFKEIPPVLEDANQQEFPSNDPNNFYFTVQSAFIQQKQHSMSHEFNQYIISQPPIYYPSYQDGFFSQKRTKKLQSSTDQEQSISQQCQDQYASLIIQQSFIQGNDLQRDKIFKAIKDDLLLLSKHKFANYVIQKIIENCNQNIRTLIFEQLHPYIIEMCYDKFGCRVIQKLLDFIQNHQKIKLIQSIKNQVLNLIFDQCGNHVIQKIIDLASDAEFIIDIVTNNIDHIVSHPYGCRIAQKCLETFPNHNLQYLYISLIPLCEKLSFCQYGNYIVQHMINQGSPKGFEVIGKYIKARILEFSQDKYASIVAQKYITLASDDDIANICKSLMNDCFPPMLLILINNQFGNYVMQNFYSKCNDKQKEQIQIQIHKYEDHQFTQYGRHFLQFLAKCNAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PW33</accession>
    <name>A0A8S1PW33_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1330081</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PW33"/>
    <dbReference type="OMA" id="TCKLWEA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF744">
        <property type="entry name" value="DYNEIN ASSEMBLY FACTOR WITH WD REPEATS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="131"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="174"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="216"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="258"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="300"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="342"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="383"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8107264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="424" mass="47500" checksum="B8E4F24A0A34975C" modified="2022-10-12" version="1">MKLKRFLLRYYPPGIILEYIRSNGEQETKSIDLLNLSNDTDVDQLVEEIVMEEPIISENRKPQLAALIRKLIDKIESKKEQQFELFKTLKAHMLPLTNCAFNKNGDKFITGSYDRTCKVWDTFTGEEIVSLEGHKNVVYCIAFNNPFGDRVVTGSFDKTAKIWDANSGKCLQTLVGHNYEIVCTSFDPHSLIVATGSMDKTARLWDVETGKQIARLDGHEGEIVSLHFNSDGDKLLTGSFDKTAMIWDVRSGECIHMLDEHTGEISSTQFEFTGDYCATGSIDKTCKIWDIKTGKCIETLRGHKDEVQDICFNSTGTRLVTVSADATGRVYNVHTGECIAQLLGHKGEISKVQFNPSGNKIITASADNTARIYSETGECLQVLEGHTDEIFSCSFNYEGDIIITGSKDNTCKIWKEVNLPVKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZB8</accession>
    <name>A0A8S1PZB8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1370130</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZB8"/>
    <dbReference type="OMA" id="VIPEFCN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001016">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8108580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="490" mass="55841" checksum="8E4E77AB60B515B4" modified="2022-10-12" version="1">MNLPLDGGGGEQQIIKIFFYSYINKENMKNSQKLGGIKKLQQNCNHEDLVQQEQICQELKDEIIQILRMPQDHISAKDAIIALKSENFIESHRAIIKLQRTLSNFEQAESIAQENELIPILFHMIQFGSTYLIKLEAAQIVSILAGGRSEITRIIMQNGILQLSLSVLDGNQSELIMLIITILGKLAGESVKYRDSILQVFTMEKIISKMEQKYKSIYIWCLANLCIGRPSPRFDKVQLAFEIFAKVIMEEFNQQNLKMINDAIWALGYMIDGEPNRITALINSGVVPQLINLLPMGNFVSILRIFECIFYGTEEQIKYLLECGFIAHIRMIVDPKSREKSGDMIQTFAATETLFKVLSNQNFLISLFRSLSDDNSNLRGDALEVIGNAIQHGTNDDVNQLVKNGLIYYLLKMLDIANEKEIVSIHLKKGLEMLIKIIRKGEIKVKDNKSNQFFTLFVQLDGISIIQKLLSYKQDDVAKYALIFKQEFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZD9</accession>
    <name>A0A8S1PZD9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome alpha-type subunits domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1370072</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZD9"/>
    <dbReference type="OMA" id="IAFTICE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03750">
        <property type="entry name" value="proteasome_alpha_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="3">
        <location>
            <begin position="2"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8108462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="231" mass="25540" checksum="5E4C0F3EB91C16B7" modified="2022-10-12" version="1">MTDFALTTFSSKGKLLQIEYALNAVAKGDTAIGIKAKNGVVLVVEKKQSSILIEESSVQKIALLTDNIAATYAGLGPDFRVLSQQARKLVKKYDLKYQEEIFVQTLSRELAEDVQIATQRGGIRPFGVSILIAGYDEEGPHLVQLDPSGAYYSWKATAIGKQAKNAKAFLEKRYNADMEIEDAINTALLTLKEGFEGQITPTNIEVGVIRDDHVYRILPPSQVKDYLEELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4U0</accession>
    <name>A0A8S1Q4U0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1410108</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q4U0"/>
    <dbReference type="OMA" id="FNGQFTH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF78">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8109680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="157" mass="18386" checksum="05A58E60990B1ADB" modified="2022-10-12" version="1">MGNSGIRKLWFLDSPVETPFKSIHEINVININKSEESLSQYKGQKVVIVNVAIDSPELNEQLNYLKSLPYQVLLFPQCDHKFTYQQIADKLQGFKVFQKVEFNGQFTHPLYKFLKRQTPQLYDEKLANGRKINQDFCKFLISEEGLPIKYLLQNEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8B5</accession>
    <name>A0A8S1Q8B5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1490084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8B5"/>
    <dbReference type="OMA" id="QHHWFIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="132"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="469" mass="55334" checksum="4CFCE3137E8D315C" modified="2022-10-12" version="1">MLQFRRTIFDQNEDTVMWKSDNQPPPLPLQQQGNVLMKSYDKYITVYLEICPERLYKRLSSNQLVYCTLDKCRLKKFSFYDQVLPHHKYGITLQLRDQQETFLIDSPDEFRKWFLLFKRYCVLDKFKQKFKVLNKSKMEDPLFGQCYYNCIHSNNYKLVKIIDKANITNYQLQCLSKEISSLRKLNSPLIIFFNELYEDDSNIYIVYNHYQGLDMRNWIKENSQNLEERLVAQIMFNLLTAISHMHSRGVFHRDIKLDSILIQSQLPSVLLTNFCYSETYIPQTTYKKCGTPGFIAPEIFKTKLYTPKADMFSLGCLFYVLYFGKIPFSGSTQEEILQKNEAAVIDYQIQKQLCRKVSSSGINLLKALLISDPEQRLSAQQALQHHWFIKMGTKQQAKFHKNIIKGKSLSTIIENSVDITHSIQCQIPSSTFIEDKVDKEFTADNLKDRLQKFNSIQLKPSKHRHQYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8X5</accession>
    <name>A0A8S1Q8X5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1460061</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8111030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8X5"/>
    <dbReference type="OMA" id="CEIGQCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF140">
        <property type="entry name" value="ZRT (ZRT), IRT- (IRT-) LIKE PROTEIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="321"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8111030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="344" mass="38066" checksum="14D3A3FC5FD38AEB" modified="2022-10-12" version="1">MSDEDSNQVVLAITKVGCMITFFMLILIVGSLPIRLKAFKSNKKLLAYMGAFSGGLFLAVGLVHLLPEAAENFEQSFDDDEEHFPFAYAISILSFALILFIEKIITDHHHDHGHDDDLHHHGSNSKNTQVQDQNNLFVNGSVDAEALLEKKNTEETFKDALNTQLIVAKKASFVQMVRKSIAQDPKNSIVYQDVNTWAPYILQIAVGIHAVFEGLSIGIQEEVSLCIGIALVVCCHKWAEGMTLGLAFRKAGVNKTTSTYMIIIQAIMNPIGIGIGWIMADKGPLYTGIFVSISVGTFIYISTMETLTEEFSISQYKWGKYLIFLVAISFVSSLWFIEQAVGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCE1</accession>
    <name>A0A8S1QCE1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1500020</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCE1"/>
    <dbReference type="OMA" id="CKITITK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008504">
        <property type="entry name" value="Emc6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029008">
        <property type="entry name" value="EMC6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20994">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20994:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07019">
        <property type="entry name" value="EMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8112070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="109" mass="12448" checksum="AFFEA9454F45D0B9" modified="2022-10-12" version="1">MEEPKVELDDLEVNQERLKQNTNQLKNLRVYGSLNAGIIAGILGMDGWIGIVIYIAIFLIVSACLAIKMNFKVKEYFRSSYDAYYSGIGTDLLLFLMIWVIFHNLVNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCS1</accession>
    <name>A0A8S1QCS1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1530036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCS1"/>
    <dbReference type="OMA" id="CIACMES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="270"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8112874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="324" mass="38631" checksum="D0BAE200089AE9DD" modified="2022-10-12" version="1">MLLPRIRIQFTLSEKTIKILDLSLLGLAILTCYKLYKCDSKRNEEKQKLKRFKYLYTADECHLLKIDSKQITVQGQINNDNQFLQLKSYQEQNKGQNVLSNIYSNESILIFDPYTKTEIIGKITKDCQFDLDLFNYHRQWKYQTCVNSESNFIVRLYQKIKNYFFGKTLVENINGIDLNQYFIFKGILKQQESELMLEIDHIGLTPNLIYQQIKKNLKKYKVQEFILSMILTSLMYYFYKKMKEFIQIKVLKKELFQNKIDEIAEDSDLCITCMTNVRNILILPCFHLICCKECLDQIAQSHYQCPLCRTQITQLKQVYIDNIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF37</accession>
    <name>A0A8S1QF37_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1550029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF37"/>
    <dbReference type="OMA" id="QYDIDWE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="223" mass="26142" checksum="3DE65B617AF83E07" modified="2022-10-12" version="1">MSKVFSPDQRNLGGRRIILQQMLQHLHHVETAKSTLSQTNSSSKLLRRTPSPVFKFLDQYKEVLNTFDKVQKIQQKRPSVEKFKNNYFLKKVQTTNTEHQKNFEKCKKRIQEMNNKNKTTLNPALFFRRVDKSLINKTCPIMQPRPQKKEIQYDIDWESIAKKIPQLADPLFKNKITDLIVSNRIYSDDDYAFLVCLISSVNNAEQSKIIQIMSQILNELGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF76</accession>
    <name>A0A8S1QF76_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Heat shock factor-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1550049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HSBP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF76"/>
    <dbReference type="OMA" id="GQQMSED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070370">
        <property type="term" value="P:cellular heat acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.430:FF:000003">
        <property type="entry name" value="Heat shock factor binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009643">
        <property type="entry name" value="HS1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19424">
        <property type="entry name" value="HEAT SHOCK FACTOR BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19424:SF0">
        <property type="entry name" value="HEAT SHOCK FACTOR BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06825">
        <property type="entry name" value="HSBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="77" mass="8945" checksum="16FCFA793FA10230" modified="2022-10-12" version="1">MNDRGQQISEDPSTKELNNFVQTLLKQMQDRFDEMQGTIVSRIDDMGKRIDDIEKSVTELMNDLGFSDDENEKGKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QG87</accession>
    <name>A0A8S1QG87_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1550033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QG87"/>
    <dbReference type="OMA" id="ICYVNKK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="15292" checksum="9081CA93F38BFBED" modified="2022-10-12" version="1">MRHQSNGQKIFIEGSNLLLTDDSIHSFRPRFSKYKNFYTKKPIFEYHYVTPTQKHAKEILDKICYVNKKALINERPESSFQKRKDFSIDQCLTTRGSTQRKYIRIKSINRSNIQNKLSDQDWLAFILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QH73</accession>
    <name>A0A8S1QH73_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1620050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8114983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QH73"/>
    <dbReference type="OMA" id="REINKQH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="169" mass="20265" checksum="B7EBAF97EF7732A7" modified="2022-10-12" version="1">MQLLKEIVEGVRNPISTIEQLKKSVIREINKQHYHTIDYTHRRSISTQEAPFTVVSIKKPKTQLNTIELAPLKNKTRFMPNSTLELIVKQQKNKSLQFKQKIYTQEYKEKSEYVQGRRRFNKNVECLKQNNQRFRLSVSEQSQQLNKLLESRLSRMLRYNNNNHDNYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QHQ7</accession>
    <name>A0A8S1QHQ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1630018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000170">
        <property type="protein sequence ID" value="CAD8115182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QHQ7"/>
    <dbReference type="OMA" id="RAKYSRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8115182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="63" mass="7071" checksum="80E627D981EBF65B" modified="2022-10-12" version="1">MEAISNTNFPKLVLNGNIEIVQYVDQESFKQLGKPKAKYSRISQLPTNLQKLQSGSLVHSLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKQ4</accession>
    <name>A0A8S1QKQ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1670075</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the exportin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000176">
        <property type="protein sequence ID" value="CAD8116026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKQ4"/>
    <dbReference type="OMA" id="AFLRIIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044189">
        <property type="entry name" value="XPO4/7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596">
        <property type="entry name" value="EXPORTIN 4,7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596:SF2">
        <property type="entry name" value="EXPORTIN-7 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8116026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1096" mass="128924" checksum="5A2F92D12545856C" modified="2022-10-12" version="1">MKASIHQNSKIAMSVGDFNKNQQILELLRNFVLDYSNIELFKSLILEDCNPETTFALYSIIQKSLINGDHFTNLPHQKVSTEYFYNEYDHRLNITEICTKHIDIALIYLQQSHNHPNYVYNQVIFTIALLMRKNWVEIFKIEDIISQIINSTIQTNKIVFFKIFENLISCIRQYSYASGYIEFRKILNNFQMLSLQRILNAIHQQIQQLQGQSLLQLYTQNKTMVESFINLLYQLMNLNFNLSYYEIDIDNDGKDTSQLTNFPDYYLDLIIDPTFQKELFQDTIGFINIDQNLSLQCINMLQRIACVRLSIFHNKKCKGEFRRIMWEGLLYLYQNQDQFINYAEFSSEIHSLAVRIINNLTLRKIVKFQYLFDNFFQLIQQVYKKTVQIYGVKIMLNSPIIKLQELWNQISYQGSLHLPLNDDFNVKINNVMQIQLILVLKTFFQQNQFTEIPQECSVKKFKKLLDLTFMPFHCLFKSHIQDNINLILQMIEQLRGDNEYQCAAVISLTIVFLLHTSNTEILNLGFESYNFGQKEEPKPNLDGQSYILKFIVELIMNQQVQRNTRILKISILSFIESFITTTIDSFCYDQETKTFKNLSQIYSQLIRVDQRYQNYVLVLETMLDECFQIFTSGDPYLIEFSLKLIEFIYQKVKKYIPRKVFKVCTITEMLKQFFLQLNITCLNTPQYIKKRAVVFKIITIVWVDDCSDDYLNSLVDIYNQIKQSILIEMNKVNILKYVWDLIGIASEIDVDNIYRAFLRIIYPDLANLLTQENIELFAKDYDSSIAIITLFKAIFYNKNTRLSTENIQLILYQIYGKAMKFFIILIQQQIQTAKTQQKISDLQLKQVSKLLALMSQISGQKKINQGFFIIYNDQCFLELYNLQIQLILIYRQLLQHIPKYKKILYECFDCICQDHSETLAYKCDLNSYLDIFLICKESLKEIIYDNQGEDTINENCATLILIGTLLSNISTFLIQEASVGDINDLQMIKNKINGINLQGKQILYDIFNLSCQITLIYPKNSKIIFAIPEILYAISIFNPKDFETLLITMVTNQIGITSNPIIDQISLAFKNLQGELQISNKEDFVRKIKVVLEMYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKV4</accession>
    <name>A0A8S1QKV4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1620111</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8115105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKV4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8115105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="623" mass="71927" checksum="DEFB5B1CC28F3507" modified="2022-10-12" version="1">MYIANPNGNLDGWSILNAQFPYLTNCAGITIFGGFDENTMISRSFSNLDPHYQLELKFQILIVDSLSTADLEVELDQDNFAESMSTQINDQQNYCGQNDKNEYIKSISFQLEHTKDIVNIFTKIKQNNQQRLQWGILKMEFQLDICYIGCTYCSGPKQSDCQSWHNLFYSFENTLFSNLIEINHQTHEFSQYYECSQCERILNIDFVQTNTNTQVQLKENFLINQEIILQLYDDGTMQISMILDYGIREFNFRLYSRWGSVAQQVQIQYQLIYHVDEIEFPFFPGCLNYLNKQCLQCQEGWKFNTIEKQCQPICGNQKIEGYEECDDGNFKAHDGCFECKYSCIQNCIHCQMGICEQCASGFKFDEKMENCLPICGDGMLVPQISQVCNDGNLIQEEDCYQCRQECDEYCSICIYFYCFQCMEGFQMVNGMCNPICGDKIVLPEFEECDDGNNQKNDGCHECKLSCYDGCEVCEKGICKIDCKLLYGQGYYFIEDSCQSICGDGIIAINEECDDGNEIEFDGCFNCRYTCETNCLDCKQGECSTYQSIVVILNQKGKNNAMMGIKIQMMDVIIVLWNLVGNVKIQVMEAFAVLQIRLNLFLISDQQIIISNQFYSSSIRIQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QMZ9</accession>
    <name>A0A8S1QMZ9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1670054</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000176">
        <property type="protein sequence ID" value="CAD8115985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QMZ9"/>
    <dbReference type="OMA" id="CGTASQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05288">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF42">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE, ZINC-CONTAINING (AFU_ORTHOLOGUE AFUA_7G04530)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase N-terminal" evidence="3">
        <location>
            <begin position="4"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="2">
        <location>
            <begin position="168"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8115985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="39603" checksum="8842844E14957139" modified="2022-10-12" version="1">MRGVTLRRYPDESFPKNIDFFDLKDFPIPTNLKENDLLIKVCYVSIDPVMRVWFSGAKTYIDTVHQGQVIPAFGCGVVAESKSKRFNKGDLVLGALDCADYCVRSANTIFKLPAVKSKHDPNLPLFLSVYGVTGITALKGLHQIPKENLPTKESKKTLVVSSAAGSTGSFALQIGKYWGYRTVGIVSSRDKKDFVLSLGADACVCYNDTLDSNGVVDTDKLTKLIKQAAPEGIDAMYDNSGEEVLDAVLPAMNKGAFIVLCGATATYYTWKNRGGLKNLSHFITKQIKAEGILYFGQKNAIMESFKEMNELVMGKHIQHKEEFINGLENVTLGLQKVFLGKNLGRVIVKLDNEVPLPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QSD7</accession>
    <name>A0A8S1QSD7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1990005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000208">
        <property type="protein sequence ID" value="CAD8117440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="312"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="453"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8117440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="651" mass="78134" checksum="B8260C34FF4C3825" modified="2022-10-12" version="1" fragment="single">MESEQKENCQPLLKQRYEPFQFEETKEIVEIEQENKLFVWNTNIKELERCIFGWESESNQQIEQFYKKLKIKLHIHFYPFYLQLMEPVQNTALTYFEELFFTIATKRQSVVCCRCSGTQKAQVTECMKFCSKKVDGGNDIGMIQPADVGIGLEGKEGKQAALDSNFSIVKFKYLNVLLLQRGRLSYKGVPYYHNLLFQEDQSFQQFKLFLCLLFPVFSIIFDEDVEIKLLFNTLHFIDLQKKSQRSKDIFGLGLEIYVLRSNNYIFDFGYVQENIFGNRNSCFYSLHFVMKKSNVSSAVIYILSNMWFENQLLVSVMTFYSFFNEIVIILVIDLLVLLPKRYQRNLIHQIMKRSWLMQKENQSIPKFLSDCQNLIQFNTNCCFFNLLKLHYQVQEFNSMIHFLLKISSSIQIQYQFYERIFQIIYHNNNNNKVQKVQEMTQQLSHQWAGMVQKQMAEKLLNENQQKYDQLQRKQRYKEELETDMKLKQQQKIIQHQQDQQLESQMLETLRQRESKKLKEMEQRKIKQIETSLENLNHLLIRKKMNQERSYQSLLLEQEDIKQEKLKEIKSLERIKIHKDEESRKLRENYDSVIQQKLNQKQQIKSKLLLVQETDPDQQIQIQRGDAQSYQIELSKQLEEKKRKILEIQAKX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QUK5</accession>
    <name>A0A8S1QUK5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2350007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000244">
        <property type="protein sequence ID" value="CAD8118357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QUK5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="668" mass="78554" checksum="783843A9877F8F7B" modified="2022-10-12" version="1">MTQLFQNKRNFQEPKILHQETISVDLQKWNQTSLQHVKEKIQILITNDNYIIYQSQEGIILRKELINDDFNPEIFHNLEQILHLRWWGQFGKNQKKQGKWTATWNGEALFEVGGYYQDGLKEGLWTEPFKDYRTKAQVYESGQYFHNQKVGRWLYYYKQNQIGGGLYNKQGQRKGKWIVLGEGFWDQSQVTYSGEYRAGNKVGNWDIWLDWNERSRKIGGGCYDKEGHGFKQGYWVEVSDRFEILSQVTNIGEYKLGKKVGKWDIWHSNFEQNKKIGGGLYEKSGTEIKVGYWIEISDQYTNFFQLMYHGVYKNGKKINKWNTKKNDGQENQKMQESTYVVNLSVFRGGGCYDDKGDGMKVGKWVEVSDKFPRYPGITYNGEYKNNKKVGKWEIFWNNEEDIKQIGGGFYDDGGDELKFGKWVEVSDGFHVYSQVTQVGEYKLGKKVGRWNICYSDSEGNKTMQKYSSQCLFLVVVDYMKKVVLKLRLVIGWKFLMNLHLTFKQCIKVNIQIIKNAVDGIFGLIFQKIRQCIIIIKCFCFSGGGTYDEEGYGIKLGNWVELWDEFQILSQIMYNGEYKNAKKVGKWDLLYRYYDESEFKLIGGGQYNSDGIKDGYWVEINNGFQWTSEIIDDGEYKKGKKVGKWSQQYRDWNKIEEGFIKIKEIIYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMI3</accession>
    <name>A0A8S1JMI3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMI3"/>
    <dbReference type="OMA" id="KTTYSEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053083">
        <property type="entry name" value="TF_kinase-domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305">
        <property type="entry name" value="SI:DKEY-192D15.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305:SF24">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE C03B1.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="371" mass="43385" checksum="5FC99EE3CCF867D0" modified="2022-10-12" version="1">MGNQQTSESQSEFLDKRLDPNYGEISIYKNKANNLIIAEIKQVYTSDSSAEEIKASLVKRLQLNHKCLIKIISFDSGKMDDFCSSCIILSITVEYFNNTLQNDIHLRKVHKTTYSEQELLYIIYEISSLCHYMKTLNYEIIDIYPSRILIDDQRQIKYFDQFLDSTRLSNYYQVLFNQRDLEYIAPEQLILLSEKNKNDTTDQELVNIFCLGLMMVSLMSGQRCVEFFNQEKLLFKQDYVNQLINKYCLKYQYSNFFKSTIQSMLKLHKEGRPNYKSLLQILHPQQDSFATYLNPIGKQREMLESQFYSFHTQGSASKSHILSSDPVDFSEIDKKIQSARQKAQNTLDEIGLDLRLNNFATTQINIIEASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPU7</accession>
    <name>A0A8S1JPU7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0060213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPU7"/>
    <dbReference type="OMA" id="TCFSEHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="475" mass="56218" checksum="6780FC33C6E78F45" modified="2022-10-12" version="1">MKRLTLRRREPTQTTSCFSDIKTEQNEPKQQDERLKNWNIMFGNFLGEGLVRQKKRNKHIDPAFLREAPMFCGNNVYYSPSVRDNFEKFIKGEISACEFLTRKNQSELLDRQRPNSPKKFKLFRVEQDNNSKNEHPKQKGFLKLLKDKSDEISKKSRIESRFAVLRNLIEHKIKLKEEEDIIKRSRELKNVGVIKQMYDISDEYIKLISRRYMEKKSIPLIQKNIVNGNQFKLIQHQNPYQTLKTATYENESKNKYDPPTQTSAIKFQINQKAKEYIDELQTRDFIQNEKAKKKIFLNSQYKNIVHQQSQSLHSLRNVKQIVPFIKQNYLKNQYINNDLELIKYITDFHVEDPFHDKEIQSKVKKSLLISAGIVPHNQISRIPLKFHKQSYLNLKDGISQKSSRSMDDSLNSMYEFNVDSLYYQSITLQNKLLEKQPEGFSRTIRSIQKNETLKTEIISKNVQKLQGLKTLKIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQL2</accession>
    <name>A0A8S1JQL2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ferrochelatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0050534</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoheme biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ferrochelatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQL2"/>
    <dbReference type="OMA" id="RFLSGYC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108:SF1">
        <property type="entry name" value="FERROCHELATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8043879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="43975" checksum="F646E890D13950FE" modified="2022-10-12" version="1">MKSIYQFTKFRSSTYNQSLQYLEQTQFDPNNKTAILMLNLGGPNSFQEISPFLIRFFEDTSVIRIPFGLGPYIARLRGPAKINKQYEKIGGFSPLFKWTMVQGFLMSKPQGSGFLPAFRYGLPLQEDAIEYAFEKTEGRIQKFLLFSQYPQYSCSTGGNAIRNAIELLQKYKTIQPKLEVSVIDSWHKNKFYISAMANLLINKIKGQNLDPKDTIILFTAHSLPADFVWDGEKYPYYIYESCQLVIDQLKNMGIQIHFDLSYQSKVGLNKWLRPATHHALNQYIKNSKFKNILISPMGFTSDHLETLYELDIQLMEDLKKDIESNKKNVIRCDSLNDHPQFIAALSDLVIQGLGNKKCSSNRERCIDCKFEQCDIVNKHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRS2</accession>
    <name>A0A8S1JRS2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0040135</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FGLAKIX"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000524">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="63"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8042916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="254" mass="28924" checksum="B131157D1945EA1C" modified="2022-10-12" version="1" fragment="single">MGNFCPKFDAHSEKSSIANIKTEEVEQTKLRVTQPIQSSHTLVTSSNALTVRKSKGQISRDDFIPIDKLGQGAFGIVFKVRQKTTNKIYAMKQIRKEKIFKNKLENNTVLERNVLKQSKHPFIVRLKYAFQTNSHIFFVMEYIAGGEFYNIFEQVRVGFPENVVKFVAAEVVLALEYLNTKLNVIYRDLKPENLLLTTTGHVKLTDFGLATMRRENGEKSYTVAGTAEYLAPEIVNKSGHSYEVDIWTLGILIX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTY1</accession>
    <name>A0A8S1JTY1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTY1"/>
    <dbReference type="OMA" id="TQNAHKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="178" mass="21086" checksum="0E96FB9E5B5C1E13" modified="2022-10-12" version="1">MNLDYTKIQDNVNKYRYGVKIGNYNEETFGMDMKQDVHKIPNSTMKDTFGLQNTLLNMPRQQETEAEKEYYKQTTFSHGGLQNHLLIGHGQQSYFETRQLTSTYDLSHNQQVKPQIQIYSKYEPNKTQFQAQQKKVEVTDKVQHVIPDVQQANQERLVKKKEFTNTFNDNYKKIPLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JV93</accession>
    <name>A0A8S1JV93_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JV93"/>
    <dbReference type="OMA" id="IDAIKEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="651" mass="75881" checksum="DA8D4AA1311D2CDD" modified="2022-10-12" version="1">MIRGDSDEFKQNASSQIMFSKSSKEIQDVSTSRRDHLFQKLPSLDCTQPNNFWQIKKGGSARCIIDSERLSTASKPLTPQAQKKQKNQIIQIEENNQFDLGSSRHFQRLFTVKNKVKSSNGSVKTSRDENTSMVSSNMVLKKSQYHQTQQIQQLQQSQQSVQQQQCPFSSGKSIKDILSQFKDKKPKTGITRHSEFEKMEGLDDYAKEFGQVLVQDELIGPVIGSAHEMKGVVKALIDLSNGNKLTYHFESLKQLHMNLNITDEMFNRFKYLYIKKLIDMKISMEIIFKCAQKVEYYRGAIICNSRSVQNVKDSIRTIAKNMYAQIFEDFSLSPLFKGTKQEEQAIKFSRIFGFILGSSESTNYVMDSMRDFHKAFGINSVQYSVFKYYLSGALSKHTEKEVVWYILEQTDAYKAAVIDQDSIKDLVYKQFGIDNFCIEFIKLCQTDPYINKNFIHKVGYDQFVQHTKYFLHYAFNKQNNCFTLEDLNQLHCQHQINIQLYQSIKDKLFNLLIRLNPQRVIFQDFEEEFDIILPYIINKKLPCDIVGQTRLCEFVPLISDELKSNVEIKNVFDSNDADVTKTILKKFEYLLSGRKYFKRSDIQAIHSRLKISEAVFEEFIYIIEKVISQYDTSLLWMIDAIKEWKYIIITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWS6</accession>
    <name>A0A8S1JWS6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0110295</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWS6"/>
    <dbReference type="OMA" id="QHENTSI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000184">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="377"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="119"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8047172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="556" mass="65524" checksum="42BE2EDB7C068E27" modified="2022-10-12" version="1">MQKNNQQQLKNLNLTKTNKPPIDSRSSRNVYELSADKSQINKSFNEKKKSVPKSNAILQELFYKRQNSQEQLFNRTSNQKSQHENRSINKSQRSISPQILYQMPQEIKKIYQQINQRLDQSKIQQNNKNKDNKKYKTEESKSHKNESTSSTINDSDEQIQNLINQEEIVFTIMAVIRRKKKVINQCQNYLNRISHFLIEEKDKISQQILSKQIHLERIGILVILYKALSDTFEEDQQNLKNLLFYIHNSMILHLQILNQSNNISQSQRAVIQARLNKIRPQKNSQNLDINLIRKNSNVVYSLLVLFIENSTDQKLSSLEKNLANIDRLSLNQGTEFIKQEYQKILAHIQQLRLTMDSQDFQFDYEEVIDCQQIPYLSKTNNYTLIIDLDETLVHYQELVDEGQFLVRPFAQQFLKDMSKFYEIVIFTAAQQDYADFILDLIDEEKVISHRLYRQHTTLMKNTYVKDIQKIGRDIKKTIIIDNLAENFQFQPDNGIQIQSWYGDSDDQALLLLSPLLIQIVQKNFPDVRDALRKFRDQMQRNIEAGISNPHLHLSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXL8</accession>
    <name>A0A8S1JXL8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXL8"/>
    <dbReference type="OMA" id="FILVMYW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="920" mass="108526" checksum="0F88A1DA22405F52" modified="2022-10-12" version="1">MTNIQHHFKLIVTQTIFLADLIETTISLSILFILVMYWINSQISSNNETIIEIMQANITLTGIDLQQTISSIGFLFNISKLALNQQEISQQNSTLKTVTGTFTVQERSQIAQIEELTQIYIPNLPYYVIQLNSESLCASNNTLNKYDFKSRPNCTNNPKFCQLLENSNNYKGNFDLFQYMKNENKDQVVFYTLFPFNIQLIQICGLLVNDSLYQCFILDLAEYKYSEGILYTTLFTINNSVISDVSDDCLTVDLKCNPVIELNNLDLNDQNNNDIVEFLESIEKTDWNNQIHSLTRYYESSNPIISLSMVDIKVYFYQSIKIGTYDINDEFSSEYKEDLLNQTKEEQDKLIALQQNFNRWQLLATFNNQLFTNLVETKQEDYFDFFLFFQFFILFLLLTPILVNIITQNEFDQISITLNQVSMMMQMFFELDYDTLHKDLITFKENQKSSLTELNELIQIFDEFCWLIEFLNQLNKKVHDLQKLEQGLHIFEKRNSIECLELILMKLVSEHSRQKDYETAITYNKQLISILEQQKKDIQLIKSEEIMRINYKLFQSLNQLLDLLSKLLIRNKSRDRNAFNQAKTLFSNIMDFADTLEIKFTLQIKLFFKKCKLLFNLRKIKEAEQCIDQAQLILQQNYTLSKNASYQNKTFNQDKKLLQVVQQKIYFYRGLIYMSSGQLQPAIQQLMLALDYGNVYQTKLRVKTIKMLSSLLAKQEKFRSDSQILKQLQYFFQNQKRQFIFLIDSTQRMAENQLEVVETIKQIFQIMNDDDLIQVSTFDEYTHVLIESQSKISILETMGKNYMEDGFFDYLSGRQTQNQRKLYQGILESLNQGVPRKYTNYLIVMTFGENYAEQNDKLVELLEKLRDGFWHFVLCSTQEKKFMNQRNTFYRLTDEARDGTYIELFANFEEGLSKIISLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZV2</accession>
    <name>A0A8S1JZV2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZV2"/>
    <dbReference type="OMA" id="PFYLRKK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="220" mass="25730" checksum="BD0F6AD4FE4F0648" modified="2022-10-12" version="1">MIKTSIIHKSKTNVEKESEKRIKGILDLEVVEKLQSLNTIQSRKFPNLKKELNTGCIIYNFPIRPQDYRYIYDNKQIPSKLQTIQYDNVSSFDNAKLQRQSSFDNNNQSLNDNSKQLKSRIKRFSINQSISQIQKQILLLNNKNNRFLQTSLRQENQIAQLPPFYLRKKASQSMSKNQSQNIEIKQPEPSRRGKSVSHIISYASRPQTGLPSKENRYLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2H3</accession>
    <name>A0A8S1K2H3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2H3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="38" mass="4708" checksum="BBDDFEB78918051C" modified="2022-10-12" version="1">MFLYLGLLFNLIKMNQICIETIQYFHLHNEDEENKMIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3D6</accession>
    <name>A0A8S1K3D6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3D6"/>
    <dbReference type="OMA" id="WKDRYSQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="132"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="373" mass="43899" checksum="F61C08A788F524CC" modified="2022-10-12" version="1">MQNERLQRLLRSKGSSYIPQNPQAVSPFTRQTQNSTIQSNKYLTNYAVQKLQNQSTHITITPSTDRRIIPTPSRPPINILPHQSSSISPIRVVQLSTERVQQPQEIHEVHHFERPIQVVAAEEMEYKYKFKIQQLESMIYQLQVENQRIKINGGYIEQSIPIDNTFKINELERINQNLRTQEEQQRIELKQLKEELQNWKDRYSQLQKQSSNQNGFNEELRQLKKQIFQLEEDNNDLQLHLRDKDQEILKLRTILNEKDDIIDQLNDQIQELQIIQENYSRVETTLISLQGEVDVWRKKFKEKNEEASELSEKLIMAETSLEAMKKRQIVQVKEVNVTKTNNNKIGTSLAQSIEAPGRFSTNRGSHYSNKQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3S2</accession>
    <name>A0A8S1K3S2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine protein phosphatase 2A regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3S2"/>
    <dbReference type="OMA" id="ECSHEYT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001336">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A 56 KDA REGULATORY SUBUNIT GAMMA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="57459" checksum="E32090D22CE35FF1" modified="2022-10-12" version="1">MNNKAASSKQTPAVRQQPQAAATASQNRTPQPIIQNMARVTDPKHVPKEIRDQFLKKIHFCSQPYDFNDDTKNVKEKQERSQYLQELFDLLKEPNFVVNLVVPHLDLIIEMIEKNIFRPLPILKKTATNGEIGMEDDDQLIDPAWTHLQPVYEYFLQLIVNEQPDVKSLKIFITHSFIQEFLELFDSEEPREREYLKNILHRLYAKLVPRRKMIRKAINDCFYTLIHETYKFNGAAELLDILASIISGFAVPLREEHVIFFKTVIIPLHKVQTCQFYHEQLLRCSMLFLSKDPTLATFLVEGLLRYWPFANSAKEVMFLNELLEVLEVCEISKLEPLIPKLFKRLIKCIAGPHLQVADRAMCFFENDYFLTILKSYKPFTFPLLVPVIAQIADTHWHKVLQESLNALKTILKEIDYQAFDKALNNKDPKYLYIIQDAKNQKKDRQKIEEKWKNLTKQAIQKNPNFKEPIVPYSDTHIVGEHNGLNNGNITIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3U4</accession>
    <name>A0A8S1K3U4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0110480</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HIGWINI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1178"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1245"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1278"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1328"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1352"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1383"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1415"/>
            <end position="1433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8047540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1469" mass="168411" checksum="42BF2E1355780A9C" modified="2022-10-12" version="1">MSSCNQQLGGQELCNKSVVNQKLCGYNFSNNTCESRKCEHLTYSNYSVITDKICNDWRYDCILDSLGCTTYTGNCTLIKLIHQCYQYQCFWQVGKCVNFVDCQINTTAITNYECLLENADYCRLNYTKGIGCAFYDCSSIQNQIICNQSNLVDGQNCIWINNKCNFKLCSDYPTQFDCENSYGQGWSSKIINKQFWCEHQIVKCSNNKFCIGNADFPKSHSDCNNRLFTTTISFQTDQKCIKKQQLCSDYTYKDACVSRIDGMKCHWSSNTCINICEAANFNPSTNLDCFIWHSDCMSDAVNSCQILDCNGLTTIEDCTIFIAQCFWNGANCQKILDCSNYSNSILCLDTKNSKGIPCFWDGTQCKEKTCQNKPTASSSNSDCSNWLFNCYFNINNNSCIEDCNSASTSNITHDQCESYYLSKSCTLKLDVIQCVDLPASCDLAKQTQCFLDSYGNKCYFQKSQNRCIILTCQNLESNFTSHEQCNQILNECTVNSTKNGCQKLDNCSSYLIQEQCYLDQNNIECEWIHNQNKCTFKQCSFAQLIQYSAFNCHQYFGDQCTVNKNVDGCETGQTFCRDYNYVQCISDGQMNLRGINCFWDDQKSSCLERICENGPSAASSHTECALFLSTCQKGGCREKGCFDYNYASDPACASIFEDKRCVTNGYQCVLRKACEDINIIDGCTFDINLNPCIWIDEKCYTKTCQTAQVSLTKYEDCNSYLPFCTVKLDGGCIRKQNCEDYLFQEACNTDSENFECIWDVLLNKCFSNQCIPFCGDGIVSGYDEQCDDGNYLPYDGCYKCKVHCPLGCHICDGMQCKECQKTGWQLVEGICTSECGDGYLVGNELCDDGNNIPFDGCYQCTYSCHQKCIDCFQGQCMQCETGYVEDGSLCKSVCGDGYLVEQLEQCDDGNLQNNDGCNDICEVENNWKCLTQYTISSCTYAILPYILLTKLSKTDTSIQEFKLSFSEPVSLNQQGINQEQFLQLIIIQITNHNALYDVEIKPIIAINSELSDVAYKIIVNFKSNVQNPVLQVEIKSDNIVNIFGNKLLSNKATLKFRSPYKMSADQKLLISKTSVLSKIMLYSIISISGISFAFGNLEILWNVLDMLQQLSYMKFHNIVFPENLQIYFEIFNIGSFTPIVDKLLINQKFENLFNFQTPVIQAKWKFEYYKIDCFYLQYFETFLVMLILGYIYFIISYLIYKFLIFCKYQNLPTIYQRDSLLKVVKFFFFIQTVCRKYYQYFIYSGLIRIYTSNFYEMTYASILQIVNFNSENTLNTTISYLALFTLICNIFIIFSIYCYLSSNNKVTKNISVFVQGVNHKKKQGAKQYFTILLIKKTIFIINLVVFQGLMGYQSLITACISGVFSCYFLVYQPFENHFENIKTTITEVLIMVNTLIFSVYEILQLNQNRELAEHIGWINISCFTLILISNLVIDILQQFLRYREAVIDKVKICLRMHGKEPKKQIALFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4P0</accession>
    <name>A0A8S1K4P0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dynein regulatory complex protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0090345</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DRC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4P0"/>
    <dbReference type="OMA" id="LDFMMAR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060285">
        <property type="term" value="P:cilium-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003352">
        <property type="term" value="P:regulation of cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039505">
        <property type="entry name" value="DRC1/2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039750">
        <property type="entry name" value="DRC1/DRC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029440">
        <property type="entry name" value="DRC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21625:SF1">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21625">
        <property type="entry name" value="NYD-SP28 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14772">
        <property type="entry name" value="NYD-SP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14775">
        <property type="entry name" value="NYD-SP28_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein regulatory complex protein 1/2 N-terminal" evidence="5">
        <location>
            <begin position="36"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein regulatory complex protein 1 C-terminal" evidence="6">
        <location>
            <begin position="554"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="34"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="156"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="271"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="591"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="34"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8045318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="642" mass="77227" checksum="62996A9E5B0AAC11" modified="2022-10-12" version="1">MDSKKQAKGSEQVQKSRGNIDMLIRTKKEELYTIKQKNDQKQKETRAQEEFKRAERANKIEDESKKANSKNAELEQEWCNLQEKDECEELNKLINQQKDKFGQIMHAKDELIKQFLDELNKKDDDFGKMIKEQAIDIQTLIQKMRSQFFQLRECIHEDLESIENEYIEERANLLNNYQTEIRNLFDKRTEKEKSFVDERERKEEEYTKDIEKLRIQGLKSYAELKISMETEIQNLEKCYEDMKALYQLNTEKLDYNLKVLKEKQISHTHTHDDLKRKDQNLNNRLRSLIKDYNEFDTKFKQTNKELTQEYKRITRQFKELQRKFKHFDKADQKAYADIHSMNELEVRKLKAKILKCDKTIHIQQLGVDWEEPQDQKETQEKSQIMAEEEVQLPLSEEKLQEIVEILIEEVDFLIDDKMRETLEKAEQQEISSVKLGVIKKCLNIDSIDEMLIFIDELIKNCELKVQEEQPQQEAVTEAIKKVQIEQPQTVPDEAPKINYETFILNQNSIINFLMHWMKTNEQRKKQIEKMSSRRAAKQETERQKKERIAKEGKKYWEKLTQVLPEKTFRVWRILDKQLSKYYELLLKRSKLVEETGQIHNHNEELKNLLNQYLQINHELKIPPTRFLKLEQQQDNVKLQNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8C2</accession>
    <name>A0A8S1K8C2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0130014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8C2"/>
    <dbReference type="OMA" id="FMFQPSA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="29639" checksum="87F1B45C7462322E" modified="2022-10-12" version="1">MSQEEYQKQEEAPDANAQPTLGQVQQPTKRKNKKRAILNPQQSTGHWSEEEHKTYINFLNVHRSVMESSDQKKTSKIFKLMSETIGTRSPSQCRSHHQKFNPFSHQVKKRQKGAGRKKKESNSQIDKSNNGFIENACFPQFMFQPSAPYFPQQPFDFQMMQLQQVPFMMQYLDQANKDDEKLVQQDPSVYLQQFIMNPQQVYDEKNQFYNFGFPHPQQMQFNFNYFQQLPNELYGTNSAEIIQKDNQSEDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9Z0</accession>
    <name>A0A8S1K9Z0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0180179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9Z0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8051561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="77" mass="9027" checksum="2C15DB64C155359A" modified="2022-10-12" version="1">MKQQKKIRKTHRRIIQEDCLDSWVNTNEYLLSKFSPPSCIISQAPGSMLEFLLKSSFYINIQISNFINYQNSVISEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB34</accession>
    <name>A0A8S1KB34_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB34"/>
    <dbReference type="OMA" id="CKMSMLK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="504"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="808" mass="95817" checksum="5452E358BF83E76E" modified="2022-10-12" version="1">MNNNNSLILEYHLYDTIQLLKNNKYKKFIKLSKKLVCNPYLVNDIFYIQLNIQLIRRLLYCQLKVVQKYFLNFSSKQQTILEVLITCMDLCQNYQILLSKYETCFDEKEIKEKQISRKLKFLNEPNKLTIKQFANQFDKLEPDNKSTIWIKYQWQQLFCYFQKTLLFNYLYCKMSMLKEASLFLFKLYKLKPISTFKSQFLYLQTLTFKNTAQYELANTTNNEVLEYGFQMLLNSLKEYHKPKSNLLNFQRQLIKITTTLLINLDIQSKSQEINDNYLDCIQSLNLANFLAFQILKPLKVKTELVEYIASEFEFTNEKFEQILLEDQDLTKFTQFLYGYHVNQKYHIKNSGNSKLQEMIKIKLQDNKSVQFQKGFQTINSNQCIYASNSQDKLTMQDYLPIKIKSYHHHKDSTVFSNPYSQTSINHTIQNNNIIKESPQTQRDMRNQNAHPGSFLSLNNVSVVKLNQEKSKKRQVSVKCKRVNTNSDHKKSYLSQLINLRQKSSLTIRNEMSELEKNHQKLINKLNNQQKSTKISTIDQFFNKKIEAIAFEECLTLDQIKDTAKKIIQTEWEIHKEMPVTKSLMKSISVSEIQLEQQQAAYNKLQEYREIQNLDYYNTEKDRAQDQMEQTKEVRKLRVNQQRFTMKHRSSQQSLFNSQQIKSLVINDTKIQEQFSQQFLQQQNTTEQRVEIKVSPRLARKNNSMLYNSNLEQDNCQKGFSLVKGQLIDLMINNLEYRDELNMQEKELKQFVLPRQFRTNAISPSGGEFSLKSQNMSDFKSFAYSTNSTQITSQNMENKLHKTFGNQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB60</accession>
    <name>A0A8S1KB60_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB60"/>
    <dbReference type="OMA" id="YLIQPQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="219" mass="25701" checksum="30D9DC91D8B741E3" modified="2022-10-12" version="1">MQNPNFMPVLIYSQPTGYPQYYPMYYMPYMSNYMIPQYQNTQESDINQIISKSSDQSQFSNQNFDPNNQLNFESIKKNKNETAVENNEENQTNTESNKPKQKSGKKKINLLQKSTNIQKNYAKAIVSYACGQRVIIINTLGKKKGIEFLKIINQLKNKLKNINHIKNYTQKESYLSMFRILGNKFLKNEAVSYIYHSNIQQKSCHLKHLKMIEQNLLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCD8</accession>
    <name>A0A8S1KCD8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0190050</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCD8"/>
    <dbReference type="OMA" id="MQLQETH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000009">
        <property type="entry name" value="Mitochondrial 2-oxoglutarate/malate carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="15"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="116"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="216"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8052173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="35160" checksum="A8CD93E8AE3BC15F" modified="2022-10-12" version="1">MSENVLEKHWLTKLTDTLQPFAIGGLSGCVATCFIQPVDLIKVRIQLKSEKLGPNAGSEISPFRVFSEILKEGGVLSFWKGIDSALARQVFYTTTRMGIYKTMYQRSKQANNGKEPSFLAKSWCSIVAGFLGSLAGNPADLALVRIQADSTLPVEERRGYKNVFDAFYKIVKDEGIVALWRGSTPTVIRAIVINVAMLGPYDEIKERLNHYFGTKDTQQTRLLASAAAGFLSSFCALPFDNAKTKMQKMKKDAAGVYPYSSIFDAMGKTAKREGIIGLWVGFPTFYFRIAPHTMITLLTQDWLTDKVNSWKKKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KES2</accession>
    <name>A0A8S1KES2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0190096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KES2"/>
    <dbReference type="OMA" id="NYDERHT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="230" mass="27365" checksum="03A0CFC99515B7EB" modified="2022-10-12" version="1">MLNIKEQKYSSSKKLLPTINFNNPITFQAWSMLRGMAFDYGRTYDFRIQGFYSLIFIGWIFLFLFGIGVLLNFIQIDYFQITLLSEMLIILTGFIGYYLWHGARLNEYYETFDILLEDVRNMYVDMLRRKEQYFILNLDITNAIHKKFVFLLKNETNSIETITQYINLIIEEIDDAIRQLNYDKRHNPFKIYGIRITLNFLQSLVVAVFTFVGYAVQQRMQSTDTACIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KG06</accession>
    <name>A0A8S1KG06_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0200228</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0200230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KG06"/>
    <dbReference type="OMA" id="MAQETQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8053241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="105" mass="12038" checksum="F546412555276671" modified="2022-10-12" version="1">MGCNVQKTKSLSNKLIQYPLKVNENAIINHNNQGQINGQKSDIKETDNFMVQQPQIGRRLKHMVSADELSKFINRKQNIPMSPFGYIKNSPKQIKKQSLMKSPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKQ8</accession>
    <name>A0A8S1KKQ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0220312</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKQ8"/>
    <dbReference type="OMA" id="EMYLLLE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004531">
        <property type="term" value="F:deoxyribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09120">
        <property type="entry name" value="PLDc_DNaseII_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09121">
        <property type="entry name" value="PLDc_DNaseII_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858:SF23">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03265">
        <property type="entry name" value="DNase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035909371" evidence="3">
        <location>
            <begin position="20"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8054971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="343" mass="38509" checksum="AEBB8888D59BE163" modified="2022-10-12" version="1">MKILLLVLVIQVFSQSCLSTTGSQVDWWLIFKLPADSNIPYSGFEYYYCDSQNDCSTMNLMNDDLRDHTSPLQRTVSQISFTSSTTMNVVWNDQPYGKSTISDRAHSKGILSASSNGQGFIINHSTPQFPVFDDTQNNIIPGMPSSSSVNGQHYFCVTMTTSQINVVAEQYIIAQTLTQKANEISTFESTYPNIYALRNNSRKIPSQSGTTQVKSKNGMNIHVISKNQNLVDDFYATIVAPILKVGLVMETWGNGTGGLQEAVCSNTYQTYSNIYRNHNGYKFKYTKDHSKFGISVQSAMPYVCMSDLNRMTTQNKRGGTTFCFLHSKIWNVINKAFIERQTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKX6</accession>
    <name>A0A8S1KKX6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0200382</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ISINDWL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF12">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="411"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1129"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1161"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1210"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1262"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1299"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1408"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1434"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1468"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1517"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1541"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2022"/>
            <end position="2041"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2053"/>
            <end position="2071"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2086"/>
            <end position="2114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2126"/>
            <end position="2145"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="1618"/>
            <end position="1753"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="2200"/>
            <end position="2331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8053542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2395" mass="282943" checksum="FDF2606AB4DEA554" modified="2022-10-12" version="1">MKQLFLNYRKNNEKKKNTYQIQFRNGETVLQDGYNENGNKIKRANLNFIIPLRTFKENIIYFLLLILQVFSSQALYFVGILLVIQQWLVDLHNFIKIRKMDQMVNSQEARVLKQWDECIVNKEIIEKIKERLNKQDQSESNQRQKSKSQVDKNSQVKKVENVQYQKKGTIVSNVSIPFQNISYKQQQSNKNIRTDNANKYNQMSPLIYKDMALKLKQVQIMEDDPLIIEIKKTVPSMFLKDSTPTAQRIPINEKLKKMEKDKMNLIKIQNVENIMIGDVIILERNQRAPCDILVLHCNDENFCVQHQLCDYSSTTVRKPVVQNRSDSQNLAQFKKSLTGNIVCHEHNFQIKGFFQLKKDPQSRIFGLENFIFCQERLLATPWVLGIVVKVGNNCQCYARFLGELRFQNTQFLPYYIMVMVIIIIQIIVYNQNEFLRNLRSITQIIIDNCIYLILMVPHFYKLYYKMCSIIQLKGIGTIDQKQIMSCHIDQYIDKQYLTVSVDAFIEQSFQLKCIIYDNKLCEFEEQKFFQFLIQICSGIKTQSEVKNVEKEEQEEIQQGSQVINYLMQSSCSDELMSQRECIINQIPKSVEGRFIYYQQSESKEDLNSNQMALIQHDQISKDKTLVENHDGYNETIQRTSNPIRNSKGYQSSKDQFKQEDYFIDEENLCKNMMRNNDPDELNPLLLQLAMNHLAFSKLLITEKKEQKVKNKFLGQLDQKQVSLAKAMGYEFLCVNNVQNVQHYIIQINEELHSFKLVITKLDIQRQRLFMLFEMDDFYQDNDKQFILFLREGNVKKNEGIDDKVWKHQYDSLHYIYYSYCYLTQESASVFLQSVESNTPDNQLYTLMEQVFKLNIILGLKYTLKPECQDFMKNIRKSDYQMFVYTQNQEIFSTSILYQSELLLQNDLVLHFNQQNEEDLRAYFKQSLQDFSNTFGHASINSSQFIKIQALSSQKQITVDQVVEEKPRTKKIIVMMNNQSLQYIMENHYFKNNLKLILSFTKVLFLYQATQDQLNTIQDIWCTSDKTINLLYENQSLLRCLSGCQLQILQLQQFSLFKNQKIEKVDKKHFFTSLITSLQQRLIFEKCYFNEMQINSNTDVIIQGYKELDRLLFFQSPLLKIFFRALYQQTFYKTITFVTSFTFVTIIYTWYTLDYMDYLIEIIFYFYIYQFFLFSLQHYSFVDQSKKLKDSKDQSILLQKYSQHKSSMDSILIMILKHIIQGLLISCIFIYKMYDLEYYLYIFMSISINDWLYLIINLNMIEAIILAALFPIIFYIYILLDMTQNDEWNQQIDAQDVFSIILGTTFLLITNLIFDYLQIQHSISIPIFNDDFLSYLNYVQMMITTKQKKKTLTILQNRVNELFENIEQVDLSIQKLLLNQQNNSSKFGQWRQQIFKKAQTILMWKKKQIIQSFQLLFYHSTFIIIMYFYQDRKDFFLVDYLIIFCLQAFYFAIQLLIKLPTNQQEYLEYGKFFLSTAATISILFVMKPVDNINSILTIEYFIVYELSIKFHPIYDFRVYITTAVATMIGYIAWYIVENDSIISNQIAVIQFLILFSVLQYFVKSYFIQLEEDQAQNKLNFIEQNNKINDILGILLPKFIRDRLNETDQYNIHQNQGLVAIVFCDICNFDQMVIEEKDKIITFLDDIFRTFDKYCQIYGVQKIETVGKTYLASAGLKACEQELTYLSQVDPVQRALNLAEQMMIYIRSKMWGYKGQQLVGKIGIHYGGAISGVIGFHKPQFSLIGDTVNTTSRVCSTGLEDSITLSEQAFDQLKNENIEFEIRNVEMKGLGIRPTYIYKCKIQNKENQFQMQIEQDAQSKEARSQYFVRKNPEIFKKDLKKRKTILTQIDTMKQKMDSMQEQGAPNVFGIKPIDPTYYQPYEDKSRHSIQLLSKQGSDENHIKQSQAKPSTFKRLVTMLQNKFQLENYEPEIDELIDYEQLLNVILLKNEYTLEHNIIKETSFIQLLQISYYKKQMNQFVSYEQYQEFVKIQSKNQTVQNLRIYVLYYMIKLFCQIQFYGNLNLGMIILQWFCCLLNLIQFLVNDKTLFEKWKLFIKLLLCFEALLSGLVIILDEDDYLKNIHIYEIIFIQSLFCSIQILSFWIKVLFCIAIAFYFTIILAIDGTQIISIYFIIVSSLYNIMLILLIEQQQVGCFNQKIIFKTQQQKESQLLQYLLPKHILNKFLDDRIGNIGICDKIDDLTILFADIAGFTEYSSKVKPEEVLVMLKNLFVEFDRKCCDLNVYKLYTIGDCYVAIGMIDYHNRNPHQEAKNIIDLAFEMINIIAQVRKQINFDGLNMRIGVHTGPVFGGVMGTDIVRYDIYGPDVLIANKMESNGVKGYVQVSQQTKKYLEEDYANLFKFELNVTLELKAIGRQIEGYLVKRQEEDNHSNQQDGPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KM76</accession>
    <name>A0A8S1KM76_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0210295</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KM76"/>
    <dbReference type="OMA" id="WAVFNNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126">
        <property type="entry name" value="ANKYRIN REPEAT, PH AND SEC7 DOMAIN CONTAINING PROTEIN SECG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126:SF61">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_2, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="84"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8054096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="19364" checksum="380A3E6879E204BB" modified="2022-10-12" version="1">MQQEEEQYSCLEEFFIDSCRFGDYEDAIGCFEDNVDLFWIDINQNNCIHMVSANGHLSILEQIIQYSKKINIDLYKLINHQNQYGCTPLHWAVFNNQLTIIPVLLQNGADIKIKNLNGLTCIEEAIQLDKTEVIELLSKNISLTEDQQQAFNETTDIIEQEDEDQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KNQ9</accession>
    <name>A0A8S1KNQ9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0250207</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KNQ9"/>
    <dbReference type="OMA" id="FAFSQIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115:SF1008">
        <property type="entry name" value="KINESIN-LIKE PROTEIN SUBITO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115">
        <property type="entry name" value="KINESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4 5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="28"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="724"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="662"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="728"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="748"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="111"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8057030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="772" mass="90436" checksum="A967C240ACE7550C" modified="2022-10-12" version="1">MEVKKKPAIELPTVETLSVLDQNEPQNGIEVICRIRPKFVGENYVNYMITDKKLDIYEINNENEISNQKTFAFSQIYDQNSTQRNLHQEYVIPLIKDIFEKQKGGLVFTYGVTNSGKTYTIIGTQEAPGILPLTLSTLDKIKTAFLLNKTTIEIDGQVIKIRESPYDDYVLKEVNYTFQSLEIYNEEIYDLLANNKTKLQLKEIYDKKCYVKDAVEKEINNTQQFKDLLNKALQNRQMGETTLNNQSSRSHTIFKIQISFLYQQEQFTIQKKQTICIVDLAGSERAKRAETQGSQLTEACNINKSLLVLGKCLKQMRCQNESDQNCRIPFRECKLTRLLCEYFTEENRILMIVNVRLTGDDIEETLKVLHYGALSVNVNLLKSKIYDSTISYNQRLQINQSQQILNDSKSKKIPKKNAKEIIRRAKMLVQNTTQHMLFQQTIQDSFVKDLISTVKQQQARLKQFSGLDDELFYPPILESDQRLQKSLDKIQSDRSRQASLDILDNKGLFQPPSVSNFLQDIPQRKSYKFEEQINQNKDAIIDERLQIDQLKLSEIQEEKQDIHSIIEQHFNSYEEQEELINELKQLDLEQIDEQTDDSKQQSKCHIIKKNNNNNQENDINISNQCQYKEDLLQYSNQKYDKNHFDQATNTTPYDEMKKKLFGQLFEQELQKHQDEKDKFKQEVMKNLFNDDDLQNLIEKENQQELINLEDSAKKQQQRNIEKLISSNHKSDLKIITESDQEHDSSGDKKHHQTKNHPKKKKQTKKKGKSKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUL1</accession>
    <name>A0A8S1KUL1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0250343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="AIKCSVF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2357" mass="274867" checksum="D40CFEB2C930AF77" modified="2022-10-12" version="1">MILIFLLILYPLKAQYSLTNKALNQSALVKKTIVEQIDIDESDQNFLTFGIWSKYSPLGIATLKNAYGIFDSNCFQLINSVDNETQSLNFIYYDCLDDVTKEIKKFIQIGFSQTEQFIFSKQIEPSQYDDIWFFFQIISYISKKKVELAIYSQLNQILKETIELAMPNRDQKLILTFGGSLKVDNSNIAQIEQGRIFSIYPGQIIVYNNEMKRISIDFDYWSKITQYFERIETCQCDYGKKNINQDSHLIYLDQLTNVSMKINCNTYTITGWLQISNIYQTSEQFTYQFMKISTILDGLQNENLATFQLFYHISPIQNKIIITTYKYDFPSVTQDFSNNPFLIRRELPIYNSITSWQYILINLQENVLEFSIIFYEGQNTQSYETSIDIKQFDNYQLKISYGNIQQSNLNYIDVIVRNLLFNNCMQDLKQYKCHYSCQECDGPNKYDCLNCSEESNRIYIPEYKKCVCPKDSIDEYQQCINFMDQNLQLNVRQRKKAKCKFGYFEIDGECIKCPSIIYNNLVTCLQCYFFVSTWQEYPYCDNIQQLTIISTNETFPDMRNFYFFDGSDLISACDPMEWQNSIQDEYGLYEQFKLHIKSFQSFCNSAELTTYDENSCYPCKQRECKACGVTIDKNFICLQCQQNYFLSNGTCIYQFKNFNYSQCKPPYYASFAKQCSLCTIKNCLYCFEYQVDYEFVINLNQIYIGYQINNPKIGCMLCEKDFNFDFNLGICVKKASKIENCITSYTNVTNEEICLTSSLDNFKISTEITLCNQYILYCSLCFLDRKQQIKCITCESGYILQDGSCYESQEQNPNYLNQYWNLKIESFLISIYNYEQNNDYTNNLTPCGYYCQSCKYQLGEHSCFKCQISSQTYQVNTPNQMCQICSSLCQLCIKRDEIPTLIMAPLLDIANINPIYTAECIIPYMDSNIDYDPYSKIVQYCLQGDCNNELTYEFIEYSCFFDRFPRNLNYQGINTEYLNSIGAVSMTIIIRIEIEDESCILFPSIINQATLKFHVFTLQTIHLKLLTTYPFYLYIFNSLQFEEYDSFTMIDYGIVFPNSEQLDLNISNSYNQVNITLINITMKDSSIKNIQSLFKTSKFGYIDLQNVSIINSQFINSSLFNFKIISLMGDIKINQLYIYNCTFINSILFEFSYILNPIQFINLTIDQCQLQNSSIFYFRANNPLDIKIFILSASIFRSNFIRSYFFYCSQQVDVISSNLILDNNQLLDSIIFGFSSGLQSSNIEISNNVFIESSFMQTIQMTNQQLASCTMINLIIRNNKLQSSNLFKFFSQFQSSDLFLKINDVIIEFNDGFIINNVDQISYIFNINAKIIILNKFLINSNKKIHTLYLFDINDIKLENFNFENSEIEEKISVKTDCLKNLNFQNQILQIVGFNKISISYFKIKQIQSMDESIIKIMSRSQYFQDQVGQIKILNLEFYGNLLQSLNSINYFSLVTIISDKTLDIVIKNLVYQNNIMHVYYENSLKDSAALLYISTLASTIKIQNLQCKQNAMTNSSNSFIYIATKSLIINNLYVKYHNILPINIWNQYYNLIPMNQDQIQSLIMQIFQIKTIGGAAYIQSSNFSCINSTFEEIKAVKSSVFDIVTSSDGIIMIQNLSVYKTRTNFQERIESTGCISINAQNSLLNLTIQNAKFQNILNRMSSSILTINPSILQNKMIFQNMLIIDCVSLKNQIVKIKFISQINKQNQIVFINLIVYQNQESWVNLFQYIGELSDSEILEITGNDNTMIYLENCDLSIENFYIEGMLLSSVFQLNNINTLKFFNCTFQNILNIYTQNLIEITQTSQQKYFIFLNSINFQNGSMYLVNNQLNQEYQQHQTNYFISGCFIMSFSVEFIQKNYFYSNLIKLLQQKQKQTSILYIRSESNLTVLVFDTIMFQNNNYSTSQQGIIHFDEIYFKIFRITKFDCYFNYIKKFGCLNIVGNQNVSHLIHIKNSNFIQNIGTQGAAIKCSDVHLKLNQCKIISNFAFTQGGGIYLLLNTKQLFISNTIIIENQAQEGGGIYFEQDNDITIKRITSTFILFNKAQVYGNNLVENPNYLQLYINQKAMPAVDLKINNISTSILKITPYKVIEQGISKLTDILMIPSTQVIDTYQLFVLQKAAYLPYIKSLNIFFKNSKGELLYNIYNSTCEVSDFIVTNDNQEKQGDITKSVLLYQTMYNNFELNTLSFRLDPYQQNYNHLSIQIFCKTQKSQQGLKYIINTKSFKCQLGEFYIENGCQICKSSQGFYSVTYDAIKCSVFDKTKFQNITSNMIDLKQGYWRPDYLSDFTEECYKNTEFCLGGWRVGDDTCKMGHVGALCEMCDVYNIRGEGQFFKNQQNQLCVSCSSEERHYYLQHYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVF0</accession>
    <name>A0A8S1KVF0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0290006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVF0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="182" mass="20796" checksum="5870E180ABE83A97" modified="2022-10-12" version="1">MAKLTTISSSSGNSSSSFSSSSSSSNSSDSSSNSGQDFHYEIEEKIEKQVILECKTDLQKQISQEKSRYFSPSVRLVSSNSTNTLFGRIIIVFCLKDILNNLKSYDINLHDCSQSHTHNIKKFDQFLNQLLLYQCFCIPSESFHTFLKPEKIFLPINKSTQINQMNKKFTQNNRSERCHLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWY6</accession>
    <name>A0A8S1KWY6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWY6"/>
    <dbReference type="OMA" id="VIDKQDC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="75" mass="8789" checksum="59E0B5A7F949F58F" modified="2022-10-12" version="1">MGCSVTKTKVDEKLKTLTTDKFEQYLITQSLFQSPPVPLRKQYSIEKNPIIQRRRLKSQQKTRTTSVIDKQEFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0K5</accession>
    <name>A0A8S1L0K5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0300086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L0K5"/>
    <dbReference type="OMA" id="IRENQWV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047867">
        <property type="entry name" value="Ribosomal_uL22_bac/org-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="CHLOROPLAST 50S RIBOSOMAL PROTEIN L22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL22M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="40196" checksum="FA834AE04A872CC8" modified="2022-10-12" version="1">MKQIAFRLNHICHAQFGLRTRAGSIIKTIKPEKKTREQIRLEIEDPFQAERLRQIQLEEDAKNFQQYIYNLKQNDSNPNNFIKVKVKNVEVTKTETATETENNTPFIVKKVPKQEKILISGIRRKVPASLRRLIPICRPLINMHLYQAQEAIADSGRKAAQFLAKTLVMVRSHAVQRGYDPERLYVHSIQVGRYKKFRKARFHAKARINPAFRETSQIKVILEERPTKEMFKDFVQGKTPSVISYLMKDQLVREKADYAKIRRFQLFLTSKGRQQQKLMLKRRAQKEMKEKGLSFKYLHRQIVEQEAEQLAENYDTGKSGLHRFNLEARQTLFKKNEEINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2P4</accession>
    <name>A0A8S1L2P4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0280136</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2P4"/>
    <dbReference type="OMA" id="GQNTFNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000064">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000015">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028934">
        <property type="entry name" value="Vps26-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12233">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 26 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03643">
        <property type="entry name" value="Vps26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8058956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="35066" checksum="927A4571767FA012" modified="2022-10-12" version="1">MASLLNMVQGQPIIEVTLDGIETRKHTRFVDKQLGVLKLPVYTGDDDISGVVEVKMNKQKKIEHLGIRIELVGRIEIINDQKQSSDFMSMSRELEPQGILFEDKAYKFQFQKFEKQNESYYGNTVRLRYYLKIYMTRSYGKVQKEVDFAVLIPQPEIEEQPQTSLKLEVGIEECLHIDFEYFKSKYHLRDVVTGKVNFYLVKIKIKYMELSVIRKEQFGQGQQQQTDNETLVKYELMDGCPQKGEVIPIRLYLSGVDVTPSVKNVNGKFSVKYILNLILVDEDDRRYFKQQEITIYRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2Z2</accession>
    <name>A0A8S1L2Z2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0320197</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2Z2"/>
    <dbReference type="OMA" id="HNSSCKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="192" mass="22676" checksum="228720DB0BAEC033" modified="2022-10-12" version="1">MQHTNIFIQSSQYIPEQSEPIKFLDFSREPYLDQSNIDTYRKQEKFGRVNSSQKKNPYRLSWANAILHKHYNVDILEKGSKIIIKKLPESEGTSQTLTNHLPVIQKSQKREKDQIFSSISQYAKNNLDKFFKQKRKYHVSTSTENNSYNMHNSSCKQTEMNDAFSLIQVKSQDRLMPIFDVNFKNNLLKYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L729</accession>
    <name>A0A8S1L729_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0310256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L729"/>
    <dbReference type="OMA" id="NENYWES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053299">
        <property type="entry name" value="ASTRA_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44156">
        <property type="entry name" value="SUPERNUMERARY LIMBS, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="161"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="375" mass="43901" checksum="DD54F3EDFD038611" modified="2022-10-12" version="1">MDEEKPGFFEQLRQLYQNREKIESKIVGFHMQKQFKISHLYGNEEQINCMDLIDISENESFIIVCSDAKIKLYSHTTKLDKLFEFVIQFEANEHLDYDGLKNEKQKKFLRHSKQLTTGPVKELIMFCHFGYIHDQLYVFAGGDLGYVYQIEIKENPEYFLLEGHHNVITGLASNNKGVFSSSKDGSIIQWDVTNRQIVQVLRDGDTPEAEVLCVSANQEHIIGAYSDGKLKLWTIQDYKNDSWTYKINKQPTIIFQGPLQDIYPHITQIFLLKQTIVTKHLNGILNYFVFDLQGRTISLRDWEMSNENYWESIDVKFPYLIYTCFNRMQVITLKKNKLTYEAELDVLPSSKLTISKISNRLIVIGRMNQYTILQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDF1</accession>
    <name>A0A8S1LDF1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0380273</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8066108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDF1"/>
    <dbReference type="OMA" id="IFENYCA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09487">
        <property type="entry name" value="SAM_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000983">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000081">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000150">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="12">
        <location>
            <begin position="17"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="11">
        <location>
            <begin position="192"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="544"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="549"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8066108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="582" mass="66920" checksum="2F129660D919FB44" modified="2022-10-12" version="1">MDYQKLFNVIPKEPRRWTQSDVESWLEFIGLQDLSNVFQRNAIDGACLEVLNDEDLNELGISSNVKKKKILQWIQNGFIEYKQFVRNNVGQSEWSIASKQIVNKENLDYTPIQRLNDFKPRRDSFQDNHKVELVESMKVVEAPQILKQPKQNLEATKIPSYPNQIKQMESIHIIELVCIGDNQKICVTEKGLSIGRNPDNTLVLSEDYVSRNHCMIEFDQKTKNFYLKDTGSTSGTFLLLMQPSLMRLGLILHMGSMQYKIEQMNCNNQQCDVVLRVIEGQQKNQKFSFQLVKNQNCLKFGRQLDQFRMDTHLSGIHAQFSYTNDGLILEDMGSRNGVWVRLSEQGQCSERVKLTQDRQFRLGYEKIYLSNSAISIKLVIVGDGSVGKTCILIRYTQDKFPTDYVPTIFENYCAQVLYENKMVNLNLWDTAGQEEYKQLRSISYPQSDVFLITFSVDEPSSFQNAIKKWYPELQADQPNAPKIFIGNKIDMRPTENVNENNFVTYNIAQKVVSDLGCKYIECSALNGTNLKQIFQEAIKQAMKKKFPQQIPQTTGPAKNQSGAQRKSNQNENADASGKCQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDJ3</accession>
    <name>A0A8S1LDJ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">40S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0360120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDJ3"/>
    <dbReference type="OMA" id="MKRIQQG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.60.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_eS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_eS17_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8064425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="16211" checksum="ED8DF1DD0AE94D4F" modified="2022-10-12" version="1">MGRVRNKTVKKAAKVLIEKYYMKLTSDFHFNKKILSEVGQVPSKKLRNKIAGFATHLLKRIQTGSVKGISLKIQEEERERRLDYVPEKSSIDIDNLRGDQDVKRMLEKAGLEIDIPIDEPVVEEKQQKQPRRGQRRQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LG60</accession>
    <name>A0A8S1LG60_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LG60"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="92" mass="10626" checksum="9D5FD8594185BEAB" modified="2022-10-12" version="1">MGLISYYCLLVVVFIGLIIYDLLFYTTYQNDVCPLIEYTDAAADETTADENAAGNRILNLMTENIDEFINSQNAAFYFIVNVWYLLFWQNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJY3</accession>
    <name>A0A8S1LJY3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0390265</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJY3"/>
    <dbReference type="OMA" id="HALQMRT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126:SF14">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126">
        <property type="entry name" value="ANKYRIN REPEAT, PH AND SEC7 DOMAIN CONTAINING PROTEIN SECG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="754"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="787"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="628"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="646"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8066725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="828" mass="96365" checksum="64B0FED632DF2B18" modified="2022-10-12" version="1">MRNKGHNKVKSLHTVQEISNTKTFFIGRITQRQFIKNTLSSLPLAQIPQIQVSAPLSGRVVARKILKGTSMKTLESVPDNEMKNQQSIFELIASSQFKNIISKGKSKKDEEKNFTLEGPLKFKDHWRKKIFKQSLNIESQSIQSEDNMNIQIFLKNIRQIITTSHIQHNEIQTNENSFLSDKFKTQMTSPRFTLTNHLSTIQFQNQPTQLYKANSTIMLDPIEEKKVQLQPTMQINRKKLTISVYNPENQIGVYSFSVPDYCKKLEDVNEEIQEYTDKPKHVLKQALQFISLQPKKMTSKFSKSIQKIIGISDQENLNEDARQFYNIQKTSRFMKMYLQNKMSQIFIKLEQNILVHLQLIQERLTSIDIIKICESQQSSRLQSCYTNPINIYPEQLSNPIFQITIEYNRFQFHQIYYFDQSFIDYSQDDLDSFDRDYEINQCQSKLKIINLTSYLNFIINTIHPETRNIMLQDENSLINNTMMQDPYDIYHDNQIINKVNSLYRYRKRRDQIAQTKIILHQYNDILTGIFQDQHEIDNLINDYGLIELDEQTQTQQSIIKIDMAKIYIKSAKPNQNKKSNHDQLSSNEIQNQNINSKKIISSPRHQTINYTSNNSKPIKQLLQATTSQTNVIQKRNDSPQDETSFKETSNLNTTQSNKMKATQTMQNTTMPISQKEEDLLKPTKSQERRNAITVISSMSKHALQMRTQITEARRRQSVPFIEKIKLLIEEQKLSELKEEFSNNPNLSINEFLKGNNTYLILAAQTGNIEIVEFLLSKGAKVNLQNINGDTALHKAIAYQFYNVADLLIAQGAQNLLNCNGMSPWQLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPF9</accession>
    <name>A0A8S1LPF9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420301</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPF9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="90" mass="10414" checksum="3CA09FB22B7B9B7E" modified="2022-10-12" version="1">MECIGQVNFLNEDQIGRFYGEKGYTIDNNSYFVQVQLIYKRQEQQNGYWCPLFNIGVLNFLSTAPDQNQRIFSPTTLMGSGLKIYIECIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQX0</accession>
    <name>A0A8S1LQX0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0420283</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQX0"/>
    <dbReference type="OMA" id="IHYSHPI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000123">
        <property type="entry name" value="Potassium channel KAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="351"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="455"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="752"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="661"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="752"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8068685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="774" mass="90723" checksum="2D5748E74D1CD37B" modified="2022-10-12" version="1">MQRPLGSPIDKKSMMQKNNMSLRNLAEKSIFAKQQLSKKRSQSINLQQQDEPSIHYSHPIETKRVSQASVPLIQQDSNECFQKVLGSKQGSKDGSGEEDDRNIVSEQRPSKIRRLSRLKDLEPPQSLKFSLIITQQSRIKQTWDAVIFIVLIYVCIFTPYKMAFIDDEEFPGWDGFDNSVDFLFMADIIFTFLCSYYDEENNLIKDPKLIAINYLKGWFTVDLISCFPFQFLFQDYDKSSNIARLSKLPKIYRVIKMVKIFRMIQLKDVEYIKVININIGSERFLYAFVLLIFSCHVTGCIWFFIASLSEDPDWEYNFISGYDQYIVSLYWAVQTILTVGYGDVKVHKWPSFLFAIFWMLVSVYIFSFAVGSLASFLDRLDHANSIYVHRLSTLKNIKKEFHITNAIFQKVKKELKSGKKNLLLNYNTLLEDLPPQLRVELAFIMNKQLQEEIQYFRDKAPTFIAAIGPVLTPLKVGAHEYLFMTGDNAEEVYFVKSGKLALVIPENDNFKFLMIKPGSYFGEIDILFYGEKRKYTIMTTKKCEFYVISKKHFKLIYLNQFRDEGAKMIKEAQKRKQMIKSAYEEAIQFIEAHGEKHFKRNQQTSNQTIQLKHMNSVSSSKSPQEISVSNQKFQLEKIIQEENEDDDQERETEAFMSLMDRQQFRKKISQLEKQIQLKDQTIQAIQQELDELMTEIQLKNLDALQEKIKVPKTFEEHLQQRRFLKANQKLKEKLKKRKSRKVSLRFSFKQTTSLSESEQNCKSLSPLKLDEEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LS24</accession>
    <name>A0A8S1LS24_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0430159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LS24"/>
    <dbReference type="OMA" id="FNISGWS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8069025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="251" mass="30213" checksum="9A24D44D17846838" modified="2022-10-12" version="1">MKNIEQQSIPLDQEFSDQNIFTYYQRSNQIRPKIRLFALQQNIIKRQPNDNVNHLQIQSSMNIIYIFMSLKTSNQQVYPNKVLPIEKTLKKNKIKLRASQLKYHKQVKNLDLQKEFQLRINQSYNLKRKIYKSHLVTQSIIKKKVYETPVYKDFNKHYTEQKPQTFRYKELDLNSYIEEYKLIKQQTIATVILPNVFQHQNRNDNSQSSRLSIQPYQHSFKPKIQNISISQNKYIQQKNFNISGWSDHSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LS71</accession>
    <name>A0A8S1LS71_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0440269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LS71"/>
    <dbReference type="OMA" id="GSTQNNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8069849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="57446" checksum="B0D8225BA4BD7652" modified="2022-10-12" version="1">MVLEIDVRILKNKETLVEILPKLYEILQTGRTLTISWPKNQKDIYYEYFKDNQKIKNLSQYQTLLNLVEATLPYPDLQSKIIVTLALYHVEQKQCLEVLKYVKMLNQKKIHQSLRQQYLFTLKYLKQNVNEIQDINQVQIIIDILLYENQISKEELKQFYEQKKIDIEKNEQINIWFIEQIWNYEKNNFKLLDILFQYFTNQNNHEKAEQIFMSFVNLQHHQYIGLNQINAEFDLCKKEYEKNLIEIDSLSTIFEDKNPTDESLLFQQQSLQSITRLIELKQKVGCPLVDMIYLYQKQAQIQKSRSDLDSAIKTLIHCLELIKAEKLINYNSRIILLESLCYDQLGDYQRSKNLLQEALQSFQDSNSLKEEIKDKCDPKKLILTKMLLASTYYKLKNLEKSLEIFYQVLKIQQHEDYESHLIGSTQNNIASILLNQGKFIEAKEICEQAIENISKKYTDSDPVMKKVKQLKEKIDVSVSSQKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSD2</accession>
    <name>A0A8S1LSD2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[histone H3]-lysine(4) N-trimethyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.354</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PPRIM_AZ9-3.1.T0470118</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60260"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60272"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60268"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="Rhea" id="RHEA-COMP:15543"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.354" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="QTFIMEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140999">
        <property type="term" value="F:histone H3K4 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10518">
        <property type="entry name" value="SET_SETD1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044570">
        <property type="entry name" value="Set1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00508">
        <property type="entry name" value="PostSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Bromo" evidence="18">
        <location>
            <begin position="200"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="19">
        <location>
            <begin position="452"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="20">
        <location>
            <begin position="1453"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="21">
        <location>
            <begin position="1574"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="42"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="1010"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="1425"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAD8071318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1590" mass="188676" checksum="00CFB9272F49F438" modified="2022-10-12" version="1">MSEQLNISNGEYFKLVEKGVKITRGAVQKQSTYTDNQNISQSFNEQSSGEQALSSSHHQSSMIQQSTHNDQQKKTQKQKKSQHKKLPNSCTSCATQVIENSLCNECLIKYEKNKYCILCKKSMFERDKAENICECRDCGLYVHKKCEPLFKDRKPNFDSYRCPKCRIELRKIIFCELIRYLTYFDDEKLLWQYDPSSVKYREVIKNPIDFDTIHKKNKKGEYLDNPELKLYSDIKLMCFNFLEFNLSNPKAHRKGSILKILYDILFQQFRAYLKLTDEDLLTYKNNIHLFTSENVQMAFRLFLNYGFGGKDLVTIEGINNYIPLWEPQLLSITQPVPSRNVKSNLIQWEKTINPNLDFENEDEPLIKLNMIDFLKQQVQREIKIKIDINKITQRHPYLDYLFKLEPPQLTDDSIDSPQLQYIVKDLLIKDLSTVSLVQPFTMKVGCPELLFEYICCSCGGYEFLNNLLMCENCNKTYHFYCQINNSQYHQQRVIKSLQNWTCNNCVKCKECDKYGLKNDLFCCNCNDFYHFQCVFNNFIAPTDGLDYWKCKNCFKCANCQNTKLFDPELLSKIKPTTTSNTVHISIEYFQNFQYCLSCGLDVAGFRFCQFCEEYIQINNDQQSNECIEIENQELGSLYRLYFQDVKQKSIEKIYLNKETKPIKQYKQCIKCKQFYHVTCLLKNKKTINNEGICSDCSQDMSQLLFKIEKLKEQNIRQTIKAKSLKKVVPVVSNFLEWAPQLNQVQQTFIMENLEQLESLVSNFFQYQQNIENLKEDLTNNKDISDEYIGKLTQYWCARYNWFEAPRQLFTVTQQKRQEFVHFQLENDFHERQLLIHFFKQKKQFVIFSLQTILGQNNSLFEFNKVNRFALDTISLITDLQCCFVQHLKYEYSFQYLDNQYYTNYLNILDQYQNSRLYRMKFLQNQKQQLLFLPIPNSIVRSIKKQYTSQMVELKKQQAKDHILVQKGWLKIATNYLKQNIKQLNDKYSETQITEKLKSYQQNNQLIKQCIQEMKQLYDNKIQEAEQILIKQEQEMKQQPKEQCIIQNEYIQKFPQLDEDQLIESPLDKIQCQLCKKNGNRSVSGRLLYVDDIRWVHTNCVLWNEDSKECELTVKALLKNNNFKCEYCQQVGSSIICGKCRKRCHFYCGYKEQWAFTNSKKVYCQSCFDSQEQCVTSFMIWRKIVVQQKQDKKSQTILHRYSNTILIALKPSEQRGQFEELNLLTLRDKILYISLNMKEFKIKYAEVNKQQFLKECEKSDKNRQLQFSVTKLVLDKYPYKEYKVQNLQELLDSFKDNNIKGIEFTENLGEFFLLYTQQVRQQLLDTKNIQKMCNSVCQFSQFKEWFYQQSFVEDLPLDERMQILQQISRVITETQVNENTIYDNHPMLYDSLPTIKHQNSKLSRKDPQQKIVTINPNDILEEANLLTEELKQIDKQEELEKKLKQESKNQKQKNKLYVAPSHIHKYGLFTKQDFKKGDFVIEYTGEVIRNALADYRELTYNEQGFGDCYMFRASKTKVIDATFKGSEARFLNHSCQPNCDSLLLDEKILIYARKDISIGEELTYDYQFEIEAESQKIQCSCGAKNCIGRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LV72</accession>
    <name>A0A8S1LV72_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0440303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LV72"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="365" mass="43790" checksum="53454213694CBE34" modified="2022-10-12" version="1">MHYLQNKKHQVFFTESKRFKRGILNTINRRHTICCLQSDLYHQIQTNLNFELFHKRSYSSSHSTTDQDSNLKEDVSKSSKDDQNNQFKKNQENRSQSESQSIHLSKQSNQKKNDDDINNIYQIRQNEVDFQQFKQDFQLDVIQLETLYYYLSRAGFQRSAPLQILPYLCYYFYSLILYRSDIFKILAQYIDMKSRDQQRQQLMELQGRFFSEKLNNQCLSQIEFYNYLIKDQLNETMVFDLLDQIIYKLNFYYNLLLDDFLKYFLLSTLQVQKIKESLGDTNIYMKNNIALGNIRFVFKKKDGELINFKFPIGNFSNIQFYDYVQFLKDFAKQLPITKSIQSFNEFPTEPPPQRKDNDIISFGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXH2</accession>
    <name>A0A8S1LXH2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0510094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXH2"/>
    <dbReference type="OMA" id="QTIVEWE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="3">
        <location>
            <begin position="623"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8073508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="959" mass="113484" checksum="9C5C5C7093728066" modified="2022-10-12" version="1">MPKQNLSNYIKFFNQINDLEQKGLITSKQKHLLKVNLTTKEYQLNNLICTIYDEQDYQSAILQYLQTIEPQPKIRYQSSGENDSPIRQRRRGNSLSRVSQFNETKLIAQIDELNDQLMTLWQSDSQFYLNHEINEKLRKVGEFITKTATSTPSNSQKQVTNITDSNQVIENLQQLYRDMKQHFNDYLRCHILLMDDKLSTEHLNNVLRAFMKNLVDADEISFIYNNEIYTSENGQFNEINLTDQQQLEIQSILEGNVIEISQNYPEITQLFQTTKFKGNYLLKIQNSTCLFCFHSKGEHKMIIESFLSLCNEYQFFDEVNQLAQFLLETLKQARVQCFNPLQLSHMIQDIGIAFIRCSKYFFIHKSISILSERYKVCKDNTSFISMHPFVEFDFRDSLNLSIHICFDLNKKEDMNVYKQLNLSFNKYLKFIKQCYDRTTFYKFFVKSQDSLIFEFDKQGQITFVSQPITSKLAEEFNINFNPQFYNASYTSIFKNKNLIQHIENQLQDQNKWKLTDSNSTYEVFMKMEEKYFKGFFVIFQQGWFRYNQKSLDANQWQKLRKSFLQQETNQYLDKLEQKHPQLKNSVVQMFKPKATQKQSQFKNFSPSFSPRHLDQRNSLQESESIRLIPKTKGINHFYPDMITETCIENIDNFDFNILPYSADWLEKQKVVFSILRRNNFIEDYKISEDALCSFLCALEYKYNKRGNQFHNYDHGVTVMQCTHAISLEIMKTQYAHLLSQFTKFALILSGLCHDVSHTGRTNIFEINSLSNLAIRYHDRSVLEQHHAATSIKLLCAPATNIIPNFTSSEFREFRKLFISNILYTDITEHFNLMKNFEARIKELNFGTEDDIKLMTGMIIHTSDFTGGAKPFKLSREWSTRVNMEFQEQYSLEGKFGYPQLPYMKDLDQLPIMAKSEVGFFKFIVRPLWSIMSKFTEDRLQQSVDNLEQTIVEWEKLMIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0N1</accession>
    <name>A0A8S1M0N1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0450288</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ICMANTA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035715340" description="Sushi domain-containing protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="2550"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="3">
        <location>
            <begin position="140"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8070486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2550" mass="271966" checksum="7BCA866D835E3F41" modified="2022-10-12" version="1">MKIILTLCLVALVCSQQVFKSNECNDCGLFKSQNDCEGYKPVACEWIGASGTTPAKCQKKDGGGSQQGTFKPYCELVEKPEVNCSKTFGCAYLDSKCVHFAGCSAYVKTTPQECQAISYRCVSDGFACIEAKECKDYNESQCEITPSVSGILKCKFDGGTCRDYGCSEADLTFTTDQQCSDWQSTCITRGKGCWTKPLPNCSTYTGTEEQCNSFIGFDGNCELAKDTTNCKARECAKASNTISTDDECKSYQKGCITTGKGCYLGTTKPLCSTYDGDNTSCVGYIGSDGVCEGDAGGTKCRARKCENGNFKTDEQCKDYQKTCVTNGVKCVSALQTCSTYKGTATGCSAYIGSDGYCTGTSTTVEANCVPKVCDQAGDTFTTDEACEKYQKGCVTTGKGCSTKAALKKCTEYDGDSTQACNSRIGSEGRCTWRSGNKCSPRDCTSAPTSTNTNPLCSNWFTNCVTTGSGCVSATTCELTVKQQSCEGTKDCVWQPICTENSSCSQFKRKAICMANTARVFDGYADVQRTIEKWVTQKCGWGSNGCENLQCSHLTGPFYSIHANCQAEMSTCISNGVDACITKVECSKLKGTKDTCQAYPGFCTNTASATETTNCTQRQCSENVDAQDNPACEAWNQGCISTGKGCVIYTTTCNQLKGDRDTCNKLFGYSSGIITNYKTVQCYNDSTATVDSFCKIKTCIMATGMTEGSCDGFLSGCAFDGKDSCVDPETATCSDYYGVSSFCEGVKIKDQGDKYCYGTATAAKCTIRRCEDNADANTTDDICEGFKTGCVAKTTGGCIAKTAIACSSQTGTSETCPNFSASTGKCQRYNTCQDRACADITNPNGHQDCMTYKSTCRFLKTGSACINHNVCASYNVPDSTATSQAKFDYCTTITDSNGKLCGYKTGNTCADRTCDQMLGTYSTTLACTSYISTTTTTGTGTCKLAGTGSTAVCYAPLNTCAYTHNISGTDDAKVTECQKYVNDKDIKCTYITGPQCTTQSTCEKIITQTDEKTCNDFLYPNGKGVCQKVTDSTCLTTVEVASGCTGYKIDQTKTEAQKKALCQSLYVIDDVDNHGAGTGKFTKCIYKSTATSTCSAISTCADIPSASSQTDCNNQLTGCFYFNGLCYGVQNTCAGATIPNSANTDTLKSLFCHSMKTASTYCKLNAGKTACEDGVAACATFDISSITGGQWDNAAITVSSYCLAVADKTKKIFCKAGTSPNCAAATCEDIPGPQNQGDCDNRILGCAFSNGKCRTFNPAGTDCTAIDLTTAAADINTPSGKTQFCQSITQTISSTATPCTYNEHKTGTADTTCAATGACSTYNVLPDDAATKLTYCNSKVDDKFHKCTFTTGASSCRDYDCFDISNPTSQISCDLGTPGKTCTYISGTCYNTTDGCAKIPTSGTDKKAYCELLTSATIKCTYISGDFCTPKVADCKGYNVTDATNKSTTCNSLKTTTNGECTYVWGNKCAALGACETYVGTTDPEQGPETGSEETQCKSLKDAARKYPCIKHDTDAKKCRAQVCADNDNDETGCASNVNGCIYYQTKCIQKTTCASYTVPGDLAGNTEKQAWCEGVTDSNNNKCKWDGTKCADRTCNDSATANFYTTSDCKSYLKTCKTNGQKCVDETSGCSTFTGNQPYCEYLLDNTAKDYCKLNSGAVVSGACQIRTCYDNVSAQSDSDCETWLKGCVTRGKGCIPRNKPCSDYRGTKSSCEAFKQFSHVDSETKLDVYILCSGDASNTETSKCKDRKCSDNTSATSNDECQAYLNGCVTKGTGCVEKTADCSAYKGTRDVCSKFMGSNGLDYCYNVSNATETSSCQKKKCSDITGTDNKTCSDGMKPFKVTDKPFCVYNGTGCDTYGKKCEEFKGTDNKDDLNSCSKYIASNGPCKATSVGTIVGSCAKRVCTEAPNTLKTDKECQDYHPECLTTGYGCTSTIKCNTLTNQDSCKSRPQCTWANLCEPKKNSCSAQSGTSQSNCVNTVITYEIDKVSYKKYCAWTETGNVCRDQKCEDLSATINTHKQCNDFDKGCTTTGSGCITMTVCSGYKTESICKNASYVRDTKTGELGRCGWENNKCRERICQDLSGKTDAECDAHLSGCKTNGTECVQALSCSDFKTQQYCLSSKSGPCLWVQSNSTCYDYDRCEDALVKTHEGCQAFSPLCTTNGETCIPITSCAKTVLKASCVKGTDGICGYLPTDKCQLFDKCSDAVSNDQNACLAYGGCITDGIACVAKGKCGDYKTEISCKNNLGSDGICFWNGSSCKLKECSDLIGTTYEGCRSQVISSGTCTTDGSKCVPLGLCSSYTEAGCYQGTDGTCIYSYPVGQTTGAKSCRLKQCEDIDKGINNSACSNVIPGKECVSNTKNCIPKAACSTYKTLEACNGGGLDGTNKVVCAFTPNSATDKVNGTCKTFTQCSDANADPTACETNKSCKKTQSGTTTSCVPHTCDTFATSTDCQPVPSFDGKGFTICTLQNNKCAPGDPGTITDPKICYTKSAYTYTWNQTTNKCEKCLAGSTQPNNTNNNTDNTTTDPNTDDYGMILSIITLGLFGIMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M2H7</accession>
    <name>A0A8S1M2H7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0520072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M2H7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="46" mass="5405" checksum="3DDF38EC7718B49A" modified="2022-10-12" version="1">MKPDKFHLWMSTTSFKIISNNGINRTIENPGLLENTTMLQREQQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M351</accession>
    <name>A0A8S1M351_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0520073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="207" mass="24487" checksum="9CE95D3E89C130AC" modified="2022-10-12" version="1">MIDDQIKDKFTSTKPXSKEQRNQQTEQNQNEQEESFWSSSMKIIQSELSNTYSAFTNKISEISTYTSTQLPNVPKELIEMKNSVLEYSSNYFNFYDSISEIKDNILEFSNKIASSIQEQLKENTNNMQNIFNNLTTTFGDYVNGINDLFEFIWQHRETIKEAFEYAIEVLKEQYDQFLNTQNFIENMRYSKEENTCVNNFIEYLANT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M381</accession>
    <name>A0A8S1M381_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0440044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M381"/>
    <dbReference type="OMA" id="RPYTEEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000184">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="445"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="150"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8069474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="625" mass="73799" checksum="60FEC39237A03C5E" modified="2022-10-12" version="1">MNSYRQNEQKKLRNSYLTNNLENSMISNKSKNSFKDLNEYRNHTPQERTGHRNQQSASKIKQILSNLVQTDQITPKSPFGQSLKEKQDMKLTDNIYSKTPKTNDSYFKQQIVAPQSTREVNQNNENLQNLYYMFQNKQQDSQSKQNVLTLSQLAKQYQQQRINNDQIAQKNGFNNNQNKDTYHSSIYINQIQQLKQKIDNSLQRKTSQKTEKYQNQNESQIIKNNGISSISKRTQQDLNCFAKTEEISDQLIQLVLQKELLWNKLYQNLEKLNYSSLITHCIELHQCISQLSERNNTQYLYDIDINIIDTFLLESFTVLIMLMEQPKGEQILDQNLRNLIIYITQIHIIAIKKTIKQSQINFIEKQFYYKIYFRIDVSIDDTTHSDVYSSLEFKQQNLDSYFIQQNKILNQISKQLQKDNNLKSTINNKSLNVLEKKKQPILPSKQTKNYTLVLDLDETLVHYQEFPNGGGQFLVRPYTEEFLEKLSKYYEIIIFTAAQPDYANFIIDIIDKQQIVKARLYREHTFLKDNVYIKDLSILGRNLNKVIIVDNMPENFQLQPENGIYIQSWTGEQKDKALKDLMPLLEQIAIKKCKDVREALNQFREQMIQKVQQGIKNPYENLQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5F5</accession>
    <name>A0A8S1M5F5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="2 11">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0480081</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="2 11"/>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5F5"/>
    <dbReference type="OMA" id="EHFNGHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07834">
        <property type="entry name" value="STKc_MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000013">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000028">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="27"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8071846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="359" mass="41733" checksum="5AED9C5C47F45334" modified="2022-10-12" version="1">MKSKQVIPKGKHAFDCNGQTFIVDDKYQFIKQIGHGAYGVVCSALNKKSQQLVAIKKISDAFSDLIDAKRIVREIKLLKFFDHENIVSLLDLQRPEHPQNYKDIYIITDLMETDLHRVIYSKQELTDEHIQYFLYQALRGLLYIHSANIIHRDLKPSNLLLNKNCDLKICDFGLARGYEDESEFKTEYVVTRWYRAPEVILNASEYNKSVDIYALGCIMAELLGRQPLFPGEDYLDQVQRIIQVLGTPTNEDVRFIGNKNALTYLKSLPKKPKQQWKNLYPHAQPLALDLLDKMVTFNPDKRLTVQECLAHPYFEGLHNPEEEPICECTFDWGWDSFKPTEAILKQMVYEESLSFHPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M818</accession>
    <name>A0A8S1M818_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M818"/>
    <dbReference type="OMA" id="NIMMAIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="652" mass="76876" checksum="2F8033234D72EB3F" modified="2022-10-12" version="1">MLDNIMMAIGWAGSQQIIAISLLRYLILVNSKALYLSQFLIRLASILLFLIVFFNKIYRLKHPRLNTPHQLGRPFRFSFSLVFFCSLMEFVSIAMAVHSLKYYSIVHFVALFTIFQSFFRSWINNVTPITVLASYLLMGLGYLLEDWIYFIHGASFDKRFLGINELFEWRPLVMVIAAAASRTLGIYIFKHIQFQQLFQNKGLIDKISWANYINDEPEKIQNSLAAVNALFSRYLEAQLIVHCEQNSIPIHQLKSDAILELLNSYFDNHLSYSYQYMEQFHIFNYNKEAVDDIDAYAEKDTLLRKMIKQRIFTIQLQHQQFKELFGIDKYQNLSFEVIYQDYGIHEYIWDEETPFITLTMQFFQGMFCLIAAFFNEEQFSDYFAYGYDIGLTLSKIEYYVWIAVFLGILQLGKIVAYKLIILWEVQTYFFSGIFLELSIIYLTMIWIESITYIKVIGLIISYIVMSISYRSAEKVRDQKNKLFYLNFVIFLVKTQKFSHREGSHIISLMTQCCEHLNLNEFLKVLSQMLCIHGIVSCEQLRHMKFSHFGESQLFKFQIPFIFQSQLRYENNIENDTLIQEEGEIEQMQKSQFTRYVIESKYQENKSDIKQSKIAGNSRIQVKSEIQPQYSKIMSDRKLSASAQKSKRPSIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M850</accession>
    <name>A0A8S1M850_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0500034</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M850"/>
    <dbReference type="OMA" id="LMHHQSK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000093">
        <property type="entry name" value="NLI interacting factor-like phosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="33"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8072806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="276" mass="32355" checksum="6AC54E8F2D1204E5" modified="2022-10-12" version="1">MNCLQYIKKCYENLRQKREGQDDSFRLSTQESEVRKKKILVLDLDETLVHCEFKENQNFNYETILDVWHKGVLYNVYLCRRPYLREFLKQMSVYYEIIIFTAGYESYCDKVLQYIDVDKHISDYFARSNCIFVNGNCLKDLAILDRPLDQLIFIDNNPNAFELQPDNGLLIPSFLDSDEDECLLRLIPFLKFMANKSSVKPVNQFLKDYETINGSLFSDTHITLQCHIDGEDGSLNEESCIIIDDVPKHRKTQTLMHHQSKSKQVQEARSQSLFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MA34</accession>
    <name>A0A8S1MA34_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0530088</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MA34"/>
    <dbReference type="OMA" id="CITQFAF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="389"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8074663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="459" mass="52643" checksum="BA12E845022F6E95" modified="2022-10-12" version="1">MGFCFLFNSNVCFGWDYKIIIVYACITQFAFMISKLLSSKGINTYCCFAIMESTLLITYLMGIVYNKEVSPININFISYTLLILQFVILAITVLISRQSLESEGHDQSQQIQEILVSAIKCIGSLQIVFLSLKWSNQINWCWMQTLVIVWFSIGTLILIEICLFIDFLMKCCNNQLENKKQISKSIITQVYGSILVNIIFIGIILTSLLTFLGLGLLLDYQISQINLGGLIVTIIYYLMELCFYIKNKEDLISFLSIQENQTQSQSQEVNNEQTSFKERLKQIHRLSINQIPQFMIKLSATYYKKKELIDSNKQQIGSVLSQDKKYRSISFSEIKKNKDIKKQASQDYDEELTKRFDHLMSSPRLKLEVSESMGAELSSRGQLKQNQLCLICYEKESNMINQPCGHGGFCQECSQQMLSKSNLCLLCRKPVTHTLIFQGVDNRESLVEVVGILQGEKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MA95</accession>
    <name>A0A8S1MA95_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0580093</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MA95"/>
    <dbReference type="OMA" id="GRHIEFS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001816">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="17"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="398"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="398"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8077398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="465" mass="55095" checksum="7C5900F28F0EA93C" modified="2022-10-12" version="1">MDQNNSLQCGLIIDQRFKLLKKIGSGSFGIVYLTFDMDEQEYCATKFENRNLHMRMMNREILILKQLKGINGFPELLHNGKDRQYSYYMSTLLGENLEVLLKKCGGKFSLQTTLQLAIQLIDRLEVFHSMNFIHRDIKPENFLISREDTSLVYLIDFGLSKYYRVADGRHIEFSQKTGVIGTARYASINTLQWMEQSRRDDLESLGYMLIYFVKGELPWSNVKAIDKEDKYDQILQVKMGIPLISLCYNLPKCFIQYFQHVKSLLFQQQPNYSHLRNLFEKQLQDYEHQLYDWELKQMELQTQDILIDPDSIPNIRLQTEIDLVPPVDEEGEVFHLKQENQHNVKFMKQLHEKYFDHNTYNQQDEIQLQKKISIEPKSQATSKQNNYQISNHTLIQVESQPQIHNNSNQFNKRKDSKSYTSFKQSQEHKENRKFRQSNDDFDLDMANDDGPNLDLKNLEVNYFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MB32</accession>
    <name>A0A8S1MB32_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0580169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MB32"/>
    <dbReference type="OMA" id="RINSCIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="564" mass="66796" checksum="4F243C9A0734829F" modified="2022-10-12" version="1">MHKLTRINSCIHHFKCIRNMKMQTQTFILQSFIFVLVFLIVMSSQMINKFMMDDIIEKLTKEIEIKTNLKQINIQSQFIKYQAHHPLEYGFKFLECFNKLNQLTLIDQTTSIHLLIECPKDEESFNIYQKMPQFCYKLTKSNTITSDKILINHIIGYQALLNSLFLPISLSDFQKTMFFTFISDEEYFAILPPKMIPTIYLPSARPWYQEQIQKVEQTQQNDVITISSIYQNFETQKYEFTITKGLTDKEQNLNGIFGIDQTLEHYKELLLYDDLNIILVDLEGRMLLSNLIIEQNIDLSKDKKYIYESDTTGFDENDWKQIVNEANQQGQGIIECNDNGVKQFCRYNTFFKQDLIIIVINISSQFYLLVFQSLAHITTNVTKAKELQDQIMSEITQFLYYCVIVAVILLLLSIIIIPIFFRPLRNIQNMINTQMISKFTIHYDYLFYSNRKKNKLAFFQESFNVFKQKLLDFTQSKSQDCYIIENFKYPRQQQMIVSELTSFIKFQIDKDKIKNLPNQNNQVVQSPKLFCDTFLDYSYNSQKSVMQVSPKDVTFRIDFQNKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEF0</accession>
    <name>A0A8S1MEF0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0610072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEF0"/>
    <dbReference type="OMA" id="NECQGVE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="30"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8079000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="377" mass="45388" checksum="EA5A2D86FEE97F42" modified="2022-10-12" version="1">MKDNYDHITTSQAIFDVVKRGNYVEETRIQYEIKCIGTEIETAQMGKDTVKYKFTITKIDLLTDTKKSHEFVRRYTHFLWLQNELQNKQIGRVIPSLPEKQTVYDKDKRKLEFVYFMQKILSHKKLQQIDCLERFFSEELRDYDAFQHYIDNMKESQSMINMVINTGISVMNMFSKFYNYNSVQIINRIPDENDKQFEEFKKELEQNKSNTEIITSDIQKIVQKLQIQARSLSNSFDIFMNECQGVEEFTTLKTIIKIYESYEIKLRQKYVYRMEASIKDYDQAIKLINLYKELKEQINKDTQLINNPNYRSQIDELKMQINNNKQKVEQLHINFLDDIDLFKQQQSWCLNDVQKKFYIDLQECYDSIKQQKTAQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MG43</accession>
    <name>A0A8S1MG43_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0580200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MG43"/>
    <dbReference type="OMA" id="VPRVWQK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="68"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8077612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="669" mass="75576" checksum="B288E9CBE423235C" modified="2022-10-12" version="1">MGCQVSNISTYMSYSTKVQEAKQGQSEIRRNVKYTNQLLTIPEQGVMNLQDLFLKSADKYAFKQCLGSFIGNDFSFMTYSQVQEEAIYLGSGIKTWGLANEVNEYMNYKMKLIGVFGKNRREWVILDIANILYGYTMVPFYDTLGPDSIPFILGQTNIETMFLSADATKSILKCKEKYKLQNLVLFDQLTEELERELKNKGYKLFRYEDVVQNGKSQIQPLTQIYPETIYTICYTSGTTGNPKGALITHANFVSAVASTQVTDAQINENDVHLSYLPLPHVMERLIIISLLYVGAQIGFYRGDPNLLKEDILKLRPTIFVSVPRLYNKFYDGIKAKMAEVTCLKKAFLDQAIKSKLSSLRSQATYTNSLYDKVFEGVKQLFGGRCRLMITGSAPIQQDVIDFLKIAVCCPILEGYGQTESSALSFSTARWDPVSSHLGGPAANTEFKLVDVPEMNYTANDIITIHKGEKEEKLAISRGEICLRGPGVFVGYYKDPQKTAEVLDSEGWLHTGDIGMITEQGGVKIIDRKKNIFKLSQGEYIAPEKIESIYNRVQGVAESFVYGDSLQNQIVAIIVPQYDYIQKQATSMQIQGGFEELCQNQKIINLFKQNIEAFGKQNQLNSLEITKLIYLEPQPLQNLGCLTSTLKLQRHIAKQVFAKQIENLYKQEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNC4</accession>
    <name>A0A8S1MNC4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0600203</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNC4"/>
    <dbReference type="OMA" id="ARDWKKV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000082">
        <property type="entry name" value="Shaggy-related protein kinase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF0">
        <property type="entry name" value="PROTEIN KINASE SHAGGY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="126"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8078693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="470" mass="54397" checksum="8BC050A4E4C2D362" modified="2022-10-12" version="1">MQQSASKSAKKQTNAIEKPSDSNIARRVQTETIDYDEAEQDIQSFQDISNIKFRNKLISFNKPAKKPIVKDFNTQFQLCLKNENSIKPISGTVKKCQKPQGIDKINTKSLLIPAKTTRQDYSTYTYQNLKAIGSGSFGTVYKSKVNETGEIVAIKKVLQDKRYKNRELQILQELNHHNVVKLKHSYFTPSDNKDEMYLNVVMDYYPESLYTYNKSFRQAQARMPEILVKIYSYQLLRSIYYISLLSICHRDIKPHNILVNPNHHKLELCDFGSAKKLIKTETNISYICSRCYRAPELIFGAVNYDTQIDVWSVGCVIAELFNGEPLFLGDSAVDQLIEIIKVLGTPNKVQVLSMNSDYDMQQYKFPQIKARDWKKVIKTNDQLATDLISKLLVYCPKTRFTPIQALCHPYFDQLRDLNQIKQLQEIYNFNIQELFDFSNLETNKMTNDEMKKVIPNWINCSSTKIVKTIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNV2</accession>
    <name>A0A8S1MNV2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TmcB/TmcC TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0660231</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ECNTVKE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000060">
        <property type="entry name" value="Sensor protein FixL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057352">
        <property type="entry name" value="TPR_TmcB/C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25474">
        <property type="entry name" value="TPR_TmcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="959"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1160"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1288"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1520"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="domain" description="TmcB/TmcC TPR repeats" evidence="7">
        <location>
            <begin position="486"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="907"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1727"/>
            <end position="1750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="937"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1733"/>
            <end position="1743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8081910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2124" mass="247992" checksum="8427D7EA9B0EAAE7" modified="2022-10-12" version="1">MSIMNHKKSQIEKGTSQEMEYKFKISLFSVMHNIIQNNLCNTLIYCVLLSIENLQLMYYPIHPVIDFLWDNQGLYYFRIILRYFQFNYLIDQGITIFIILLYISFGVMVIIGLIFFISQYSLSQLKYQEQQSGMLIFSFKLLSLLLIILNTVLALPFYNIFLATIYCRSDSAAGADLGCYQGIHILHMIVAIIGIVLLIFFHFSTTLLYFDLNPKSKFPFNGYQSDAEYARMLFKFVIPFLIIFNAPGTYNSEMVVLFGVGDLILLFSRYNYPTGYNNFIHFYKITLEIIVLWINICVIITDFLDTGEPDDMGLLYLFVSIPCVVFGGLYAFKKILKESMTFSIKNLKNEEEAYNYLNILLQLILDRKSPNTKIKLMGVLKLHYVSCTKTGCACKQLISQDHRQEEGTQENENWMKFLGSILDDLMTDEKFKDNAQFKIFYSYVCNDELKNKYNALHYMMKAEDTKNHTLRDEFCLFRFKQHIECEMQEDSEKAQDIDVNSIVFFQNQLVVLTNMIQKSAESHLEFWRELQEDYPNILKLQNIGMKVLQQIDLCEEGYQELIEINNNNLNLLEFYNAYLQLVVHDNEQHEQLSQQISQIKKTIYQRRQGGGQESKITESQETIIITMSGNVGSIGIVASCNNEIQKSLGYPASDLFEQNVSKIMPKIIGDQHNQLVENYLKTNNSNIIGLERFVLAQNSQQFLVPCKLMVKVLPNLDKGILLVGFLKPLEKLPGTHYMIYDAISQQILNFSESIKDLGIKIQNIIITSAQEQSKFSFGRVFKELGDILNNPVNLNDQSLKSLLYKLDGDVYSVETTINVKQINDTLVQINHDPDNKKMGNSALDSEHIYMNSDYKKVKLELIEERFPLTDDGQPAGGFIYHTIVIREDIDQEKVNYGKSSIKQKLDESKHQIVQQQSIEEEDDQQKNPQSSVDDQEENKQTKDIRSALEQSKTPRQIIYLRYLSLILFLSTLTLSITKLVLSLQQSILVDEGVSAVRQAHRRHSLMADINLYCRYLYLTSFGYYDQSLEGAFKANISVLVDKLQVIQFDMIEFRIKVEGRSGLQIDDDQYDVLFLLSNNETKTYGNIFNDAVFAYITSASSFKKATLSQFNDSSNSNSVSKNLFYVVENGLGVLRNGSERIADKFYNFYYDQVDAQTINAVIILITALIIIVICEIIYVPIFMSIFNERKLLISYFGMITNDDIKYLIDQGDTFILDHITRFKSSDDIYQVDQPQQQNQVESSLNQDQSQDVIESPKQKIEMDQRKSLFDQTKLKHQRIYESQGGTHYLLIFSFIFIGSLLMIEYIVMYVLEDQSISDAKLIMDHYLYISRRASIIKFNILFTLESLVNNGQPRIYFDNDLNKYYNEKVYSNEQDLFKTLTYTYPSSFDDYFTFFNNVNFLNVCQHISWIQKLQLITQSFVFVDTIDNFTNYTFSSSELEQECNTVKEGILTKGIRDGIFLVSLRNNELLARFSNFSDSLEEFQIQELLRKYILPTFWENNNIFQTCFYNFLDIRDQIQIYNFVGFVGFLLLIFLFLWISYIAKVQANIQQSMRMLTLIPDDLIDKYTKLKEAIEFHVLVLLFKRLLCISNNQTRNSQSQYFLFTQETHNGILEEGLQIIYQIKNSKFGIKGNTTSFSKVKRYEIVIHSIGRIYIKCQQTKTDRFVKKVKNNITTPSPQHSKGHNHEVHKIPGRLYKGTLLKNLENTTNSFEKSYILDSKKEFGYSSISPKSTKKDHSSEQLRQKHKQISNTDQVVTPYKELKQQLMIRRSTLNNINSTIALKKTTQNVVDEKLEGMKLECLLIKPCPNNINNLVMIQFENVLGYDESSFFGLQPDFIGSKQHEEYLVLRDHYFQRASSTSSFYIHKNIKELFNSISRVYQIGIYTLNYQQLLKDLINEKKLKINGAFQITDYKIDTFVIDITNVLTNLKIKNPQLLIFIQPFKLLNQQEFFTPNHTKIPYYDYYGQRFFIPFTKSIHPNQYRLLALSSLQMQSLFKNGEQNNGFQQINYFIEQFTLALQSESNFTQFLSKSQNKIRNRQNKDYYNKFILLILQNLIKINMMTSNQKKKKQMIDYLLRLRIQNKWKYYTMIYFIETKIQLKNLRILNVIQPTIYHNQIQNYNNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MP55</accession>
    <name>A0A8S1MP55_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0670154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MP55"/>
    <dbReference type="OMA" id="DQHFQTF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="206" mass="24176" checksum="4C2A97850615B1C6" modified="2022-10-12" version="1">MKPSQTFRIKSIKTTINNNHQQKSLQMSLKSQSLTNKTKKQKDQIYKRFSQIQPLHVIQKSINIPCRQSIASVTDSQISADEEFEIWDDNQDLRVKKIILDDKQSSISQFLQLYKARYGGFPGQRQAISNEEVIKTAISYASSSKKLFKKYFPSKFVVFEDQNDYSQPYDVEDDQHFQTFNKFFRNKKLLSTHKVSQQSLKKTQNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQZ5</accession>
    <name>A0A8S1MQZ5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0660162</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQZ5"/>
    <dbReference type="OMA" id="VGYDAQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20621">
        <property type="entry name" value="CYP5011A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000091">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF50">
        <property type="entry name" value="BIFUNCTIONAL ALBAFLAVENONE MONOOXYGENASE_TERPENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8081772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="506" mass="59653" checksum="C671E27BD1D8FFC9" modified="2022-10-12" version="1">MIIYVLYAVLGLISYLFVIKPLTEMIRLKLQFGKDCKISFNFFGKEIYDQLKETKESKNERDIFNKQKTAYNKYQYKFFMTNMLWIIRISIADPLYQKEALINHQLYQKVDPVCHNDLLSKGLVWSKGEQWKKQRLLLSSSFEFDSLKKKVPMINEITQSKIDNFSNENVLSSLQSITGLIVIKSFFGIQTDQIYINNTELQVEIANIMNEMGYMRFKSQIQSTKRLIFGTKAWKIFPTKEEKKLLQRVKDMRSIVENLVQQRIKYFEDSNNSQINNNENFLNILVNEYLKIKNKKQQETTLDQIIQEFITLQFAGTDTTAVLLYHCLYFLACYPQAQEEIRREINESCPSSYINENEINNLKKLSAFINEVLRLKNPAMRPIIRVATQDHKLKDLKIKKGWLVCIDYFLQNQSEKHFDNAGQFDYTRWLQDKPIKEDNNFIYMPFSAGPRNCIGSQMALLEAKIILGQILKRYQIMINKNVEVSWEIHFNHQLSPTNAVILNKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MS94</accession>
    <name>A0A8S1MS94_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Arginase</fullName>
            <ecNumber evidence="2 10">3.5.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0670126</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>L-arginine + H2O = urea + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:20569"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="EC" id="3.5.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the arginase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.1" evidence="2 10"/>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MS94"/>
    <dbReference type="OMA" id="VMQEAQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004053">
        <property type="term" value="F:arginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006525">
        <property type="term" value="P:arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09989">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000012">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014033">
        <property type="entry name" value="Arginase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01229">
        <property type="entry name" value="rocF_arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782">
        <property type="entry name" value="ARGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782:SF3">
        <property type="entry name" value="ARGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0056">Arginine metabolism</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8082182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="323" mass="36362" checksum="4A2022DB55EFB03B" modified="2022-10-12" version="1">MNQWKFIRNSIGLFGSAVHEGQTKTGTELAPRALREGGLISALYNLGWEINDMGDIQGKNYKINNVKSKFFKNSQQLGIINGEISKRAFDIVSKQKFMLNIGGDHSVACGSIHGLLEHYGDELRVVWVDAHADCNYQLDSNRNYHGMPLGHLYGAITEQIKGFEWLRHRLDTKNIIYVGIRDIDPMEREFIKDNKIVYYGMDEIIELGIGQVMTRILKQFEGKPIHVSFDVDSIDPEFAHGTGTLVDGGITSREVHYILRKLAASRQLVGMDLVEINPQLEKTPELREEFFGDFQQIGRIQGTQTVALGIELIASALGRTLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSC7</accession>
    <name>A0A8S1MSC7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0630028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8079973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSC7"/>
    <dbReference type="OMA" id="SWRMNEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="634" mass="74244" checksum="F676FFCB777CC480" modified="2022-10-12" version="1">MQQLLKEGGNRNQKQNQFEWRSITPEREFGQMLQGKPLITEPNENKQTAKSMSQENNINNHDFKSKHTNLNKLLIHDNQQQKFQRPLNYMQTSLKKMSEIGQQINSILQKFKQNRIHRPQSDLNMFDKHSMMKKTSQNDISLSTNLNLNHSYIDNRGTETERSYFQYKNHKDNGKQQNKDQKDYQFLFQNSNNQFQTNSLKKIIEKKIALAMQTNTNNNLYNDYKKQINKESKQDRSYNIQKYITNKFLRNSSCLQQNEKSDRKNKQMSSYFSQNHYSKSQSSFLIERENSKSENTTSRNNRIRVSSYIKTPTKNTSDLIKQFSPRFISQQLSSYQVSSSKINSLLSSMNKDIEKIQTYNNIQNLNIYHIQAIPLFLSNDPNQTHVIVACSDLSVRAINLYSGRIQVLGKHQKPIRSITKIVIQQNFKTFYSCSLDNQIIQWVSESMHGTYRLKHSWRMNEVPLQLYQTEKGVLYIVTANYIFYFSSVTKKQITYHGVFNCMIQTSSNILVAIDNFIYKLQADSLQLVCTSKKPIKNMIYEEGLLYVLQRDGVLLIYEEAKHLKFNSEIDIGAMNIFSMQNGQIIIQTNDSEFIVYSQQISKSYQIDGKIKCLLALQQILLIGSNKKLIETLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MUK3</accession>
    <name>A0A8S1MUK3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0710037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MUK3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="42" mass="5233" checksum="D855AAF71B3EFF01" modified="2022-10-12" version="1">MIQLRYFNKLLRNKKKKLRQKLLRSKIQSILKRKNNNLIHQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2H9</accession>
    <name>A0A8S1N2H9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0690214</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2H9"/>
    <dbReference type="OMA" id="FHAECCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000612">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052788">
        <property type="entry name" value="RING-type_E3_ligase_ATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798:SF97">
        <property type="entry name" value="ALCOHOL-SENSITIVE RING FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="257"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8083285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="37819" checksum="3A9E564E63E6752C" modified="2022-10-12" version="1">MINEANFFWNQDYTVNFGSEHSYLDERLIITDDYMPMLPRAIKEGNALCLFLCFYYLIKLFITLSQYQQIDEPLFSILILVMGFHDVINILHYLIIGILTQYIGGIDIQNFNRQLNLLSLNSSITQILIQEFISYYRPVNGLLIIDIIEQKSFYFNCITIKLLYYFISVYTFIIILFITTDTPLLIQIYIILLIIYWLIPVLMCFIWPFVMLWFIVLRQELHYQQNRNLIIRFYDQLQKIKFKKLMEQRPSIQQNDCPICYQEINESHSIIQLPCHKEHYFHAECCKQWLGRDPRCPLCRYGLEQQKDVALLEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5U2</accession>
    <name>A0A8S1N5U2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0670242</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5U2"/>
    <dbReference type="OMA" id="EDFCIIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="358" mass="42112" checksum="3ABC4B38C774CD76" modified="2022-10-12" version="1">MNFENIVKYIEKSESCLNYEIITAPFVPNTFSLWREVVYENLDPDQWFEFVKQIDKQIFQRYLEKVREAYAKKLTYQDLKLNVRYDIDVKKLESIFENDLKKQVDTITVHLSYYQAQVEYAYDLLRRIHTYKFVDYNVQQFQTSQKDQTLYQKYLDKIMKGFISNVQEIATEKIKEFCATGLIKNNSFLESGKSFFKQFLFNGGVAVAKLSNLNLGLFIGGIIAEQIVSKVALSMMTQRISSKLDFLQLELDQMTNELTYINSDLDHLIYRAAEEYPRNFEQNKTFIQAFVQKNYKVNLGSDIIYCPDTNTIIQKSVEEDCVLLNEVEYNLSLQEEIDEDFCIINLASNIQDSINKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7I6</accession>
    <name>A0A8S1N7I6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0780024</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7I6"/>
    <dbReference type="OMA" id="SAGMYPQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21383">
        <property type="entry name" value="GAT_GGA_Tom1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03561">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13856:SF137">
        <property type="entry name" value="GH05942P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13856">
        <property type="entry name" value="VHS DOMAIN CONTAINING PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VHS" evidence="2">
        <location>
            <begin position="12"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="3">
        <location>
            <begin position="172"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="450"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="502"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="548"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8087089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="65545" checksum="5737C59251F6CBB5" modified="2022-10-12" version="1">MENPVERAVNEATADTLQMPDQKLMKQVADLVNQRADQSKFAVQAIGKRLMQLRNGKVQALSMELIEYLAFTCETPFYTQIATNDFLQRLNTLLNPQMNAQMQQRLLQVIAVLKSLMQSHQDLFPAFFQFLQKIASKHQIPQNYESKYAVLRKSQPVYSSGARTNSLGSGSSKNDKLRRDLEIVKSNITLTNEIIDNANPQEDASKNEILKDMTTTLRGVEEKLRNLITDMGNNDEGLMNFCLELNDDLLKTFTRYEVLKKHKKPDPFRPELQQQQQQQQLPQQPPTQPQNIQQSPFQQQQNPFSIPPPTQQQTQPQQIQQPPQPKPQQIDLLNLFEYDEPKPVAPQKGPQPTQVVQAEKQQNIIDLFEVDMSNPQPQQQQQQQVPQQYQQQQSQIPPIQQIPPISQIPPLSQIPPVQQFVQPPMQSQNYGQAPISQIPSQSNYSVLNAFPPQQQFPPQQQQQYPPQQQYPQQQNLQQFYQQQQFNQQQMKYQAFQQQPNQPIPYQQTQPFQQQGFPQQQYPVISNISNQQKKPENEFDNILNLAVGQKPQPQQQTQQQQQKLDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N940</accession>
    <name>A0A8S1N940_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0790084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N940"/>
    <dbReference type="OMA" id="LYCVDER"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="377"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="561" mass="66776" checksum="F548AC50737DB955" modified="2022-10-12" version="1">MSDQLFCVDERCNLSQKLISKWIDFIQHQELEHKMLTIQQFKAYLIQKKINNGPQFSDQRKNQFFSQAQESLNVFFDQIETRIQQFVKQELQEIFDLCLKDIEIQIDDCIQNDIIDLNNFETYFQFEEYQSKCLNFYNNNIQIQIQEIINQFCRQKDTQFEDIFKSLTKSFIHQKNMFIQIEESKITTFQPQIQQQKKENIEEYQSLKGYQQIEKLQQNYFQSKEYTQESKPEPLLSIGGQKQNDQINQDYFQFKEYTQESYLESSNSLKGHKQIEKLQQDYFQNRDYTQESNTESLLPLKGYKQVDQKQQEYFQYKEYTQAEPYKQVQKKQQDYSQNKEYIQESKSDPKITKNQTIIQDPQQQNNNQRPKKKLNVNQKQNTGNQIQQTQNQISQNQKEVKIPQFKQINGKVEYFTPQLMNGVVQLLDNFKTAKSQSGFILFDGFFTRSSTIKLELLILEIDKQSEIEIGLVDWSIFKLPRETGKPQKGVYSLNNKGSVFKDVKQKNKDKIFKNQTKLKLNIMNNQQQFIGEKTIGFDISQLKGNLSLFAFLQNGQIQIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBB3</accession>
    <name>A0A8S1NBB3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0830180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBB3"/>
    <dbReference type="OMA" id="LANPTFC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="274" mass="30292" checksum="A4D73C0A0A246523" modified="2022-10-12" version="1">MSITVSTCQQICQSPLNLSPSKNLWTFSKSERFGKLANPTFCQQAFYNLPTMIEKRSAGIGKGNKIDFTKVVVPSPSPQQYDLGSDVQNNLKKNKGYKFGVSRDKMASTGILGTLNLKTPAPGTYDLGSTLSDIRYTMRPKPQKSSLISNAKVPGPGQYESLPAINEKGRYPISKYSNSCATLFNPKSSKRFTTDYSTKVPGPGQYGLDKTGIQQDGRYFVSKFHDSNVRSFPKEARRTGSNEKYQTPAPGNYRLPSEFGYYEASKSVGHTEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBE9</accession>
    <name>A0A8S1NBE9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0830200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBE9"/>
    <dbReference type="OMA" id="SIRDFRW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF434">
        <property type="entry name" value="GB|AAD18119.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="264"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8089452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="318" mass="37625" checksum="D159408ED4EEA0E0" modified="2022-10-12" version="1">MNISTSCAHSIRDFRWKNIILGCFSLLGLIFSCKSFVLDSNTFYQLIYISELIIGYFMMLVVFINQFLAMRQTQYVLTMSYLINETQRVNVQYTDQELEEMIKDECRNHVSKCSVFYTSIKMCFHNFCCESKDFLYLVLSAGVIISYFFEQRFQQEDIGGKVFILFCVTFWDLLLVTLGFIIEMPRRIRQYYKMRALHRRATRLQFLDLQRQVQSLGDNNLEGQQGLYIDLLHHDLIVDQNNEQRQNGFQGQNAINAEDEQVQCQICFEDMNENTNIQQLQCHPSHIFHSDCITSWFQRKRQENLVPSCPICRAPQNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NE64</accession>
    <name>A0A8S1NE64_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0780178</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NE64"/>
    <dbReference type="OMA" id="ANCMHAL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000113">
        <property type="entry name" value="Beta-adaptin-like protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015151">
        <property type="entry name" value="B-adaptin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09066">
        <property type="entry name" value="B2-adapt-app_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="6">
        <location>
            <begin position="37"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-adaptin appendage C-terminal subdomain" evidence="7">
        <location>
            <begin position="666"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8087395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="778" mass="89775" checksum="5E7E8D268D4F7B10" modified="2022-10-12" version="1">MRPHYASSIASIPPPKQQQYFEGNRKGEVNELKILVKNTLSEKDDKKKREVVKKVIAYMTLGIDVSKIFPEMCMASYTNDMVQKKMIYLYLTTYAEQNKDLAFMAISTFQKDCKHSDPKIRGFALRNLCSLRFSGAIEYLMPAIRESLSDIDPYVRKTAIMGCVKVYYMQPEFLSNIEEQLYKMISDNDPLVIINAIHALNEILAAEGGMALSKKLVDYLLGRLKEFNEWGQATILDELSKYQPKDDKEMFNIMNLLEERLKHSCSAIVLAVIKVFMNFTKNKPQVYEQVITRVKAPLVTLASISEGNLEIMYTILCHIKFIASKGYNQVFSQDYKCFYCRVDEPTYIKLIKLEILALIACDFNLGDMLNELGEYVTDVDQEISKKSIQALGAIALRLPDLANAIVKQLSSFITLQDYITNEVIIVFKDILRKDPKHIKDCLEIIQSDSVTDQNSKIALIYILGQFGSQIPLAPYILQTFIGVAESVELKHTLLTSCLKVFFCRAPEMQEILGQLFYSIMNNENEDIDLKDRAAFYYRALQNNPNEIKKLWQNGIKVDKFLEEQNINKEALLFEFNSLSVIYEKSVNKFIKPAEYFNNLRNKELQDQIQEQQQSTTAEKPDPVQDGFDIEHDITVSNVPIVQNQPNLLEISEQPKQKLIIDNFVLDYRIEVEYFESMWTSLNNGASITRTLARNDIANENAMEQLFNRYRLYNIAAGEEDNVLQMYFYGAHKCQVFFEFEINRNLNTVLLNTKSELEDYAYLAQKYVEDFLKQSNLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHL3</accession>
    <name>A0A8S1NHL3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0840022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NHL3"/>
    <dbReference type="OMA" id="NCAMSKS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF140">
        <property type="entry name" value="PLC-LIKE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="318" mass="36614" checksum="C69D190759938437" modified="2022-10-12" version="1">MPGSNGHLNDDLQLNQIVYLGAHNCAMSKSYGWIYAQQNVTITEQFEKYGARHFKVPLHWYSNNNDPEIVIAHESDGKSNCQLTLMQRALKKPESAHLRLKEIVDLSFQNPNEIIILKIESKLLCNSQKNGTENFDPQLTSIILHSLLVDIKADQRAIRIQKDSEIPTLGWCRKNNQTLLITIEPKEQIVGELLKYTYYSYFLSAQVDWEADPLKDVDNGNVARGKVNQQTGEILSPFLEIHFCPQNSLKLDSTYRDKYNSYNHVRQRFLQYFFNCIKMPNFLVADFIDQGKLSDIVDDVNLIINSNGIFLPELFQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJK5</accession>
    <name>A0A8S1NJK5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0880126</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJK5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="436"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="619"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8091549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1230" mass="143652" checksum="735ABA34B0E756B1" modified="2022-10-12" version="1">MINRDAGFQDLESGIQSNNISSGQLFDIDQPSPIGGYKLDPTLEFSQLQQLEALKLKRDIFDNNHKQAINLGHKADVNEEDCLISNLSGKRSEPQGSISSISCNLKIQKPLTVKTTQNKSNKSIQIQQKFDQDDFIIPSPIENNGTINLGLKSDAKIAKYQTFVQQLEEEQEQNERTKRAQVNRDTRGRSKEMKTGLNEVWTQKAFIIIRLVSRFIQQLKTKTERIKFRLITKRIFEVICDNSANFEYMLINRLIKQKPSISLIIFDQIQNKALKLLNSLEAINAFFGKYVKVIKPDSLFKIVWDIILLLFIVVNIFYIPIYISFDVRSSGVFEWFFDLLPSWIFIAEILLNFNTAYYDKGLMHEDRKQIIKHYVKGNFFWDLIVIIPFLISYMDIPFVRYTLLLRLTRLSPLMTSIEEVLNLEDNMQVFLDLLKLIFFLLLTGHFCGCAWHWVAVIEYQNYGEELTWLTKYAPDAMDYYWFDRYIISLYWSVITTVTVGYGDIVPVTTVERIFVIVVTLLICGVFGYCLSNIGNIFKQISDKKAIYKQRIREINQHIRKRGMSYNLQLKVKKYFEYFFKIKQEEDQHAEQFIEQLTKHLREEVLIDIYSKTLTQSRFLRENFSDETIHRLCQIVKETKLYPEQLLFQRNDSPKSLWFVLSGAVEYVADHQNEDEHFYTETFLKKITQGAVIGEREFISQTPYEYNARATKFTQLLVVEQYTFKLFFIYNYKAFGQICEICGWTHRFIQCPFVFLQPNKNKIASSFASIKTNRRLTFPYRTLPKQNWKNNLPDVQEAALGYIVLNNIIPEKEINDAYLINLGFELNDKDEDQQIQVQNKKNSMFKDQKVLSQFDNNLQQQPVPQPSIQTVLPADQKSQNQTAIFLKDGQRASMTNVKLYILNIIYQESVIDWDQGSQFRRSLNRIKLQGIDKGKTLQQIKGKFHQNGATDLLDESMIQEEVKIHNANVGSQNFIHSKLKIVKNKINKNQNQIGIRKVSWLEFDSAINQNLDNKPIHLLKQVSKGSETNFDQSLPSCSQEMEIKQRIEARENSIISKKTSNVEVTRRKKRRRTTQLLQLFQGSEIDNKSNKKVDKSLLNNEAYSSSVYTAGVPSNSAVVESNNKFDNQGDSNSVHAGQSIDGIQKRIQDIVRVHYEMEMDVARSSKIYFPDFNLDVVIQRITVYYEKLNEKKQEFDMREMKRTKTNFTLFDRIRQAKNTTIKGNFVDKEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NM12</accession>
    <name>A0A8S1NM12_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diphthine methyl ester synthase</fullName>
            <ecNumber evidence="4">2.1.1.314</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0920173</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 4 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="EC" id="2.1.1.314"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the diphthine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.314" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NM12"/>
    <dbReference type="OMA" id="ICDERCW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141133">
        <property type="term" value="F:diphthine methyl ester synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000004">
        <property type="entry name" value="Diphthine synthase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000004">
        <property type="entry name" value="Putative diphthine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8093202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="278" mass="31523" checksum="159C851F3389584F" modified="2022-10-12" version="1">MVFFIIGLGLGDHLDITVKGLEAVKTCKEIYLESYTSILGINRQKLQEFYGKEVIEADRECCETGIDKILENVCADPQNNYAFLVVGDPFCATTHTDLFLRAVKLGIKVEVIHNASIINAIGCTGLQVYRFGETVSVPFFTEKWKPYSFYPKIKANLDHNLHTLVLLDIKVKEISEENLAKGKKIYEAPRFMSTIVAVQQIIESDKELGLNAIDEKTKCFGVARIGFETQKIVSGYLHEFLDIDMGAPLHSFVICAKELHPIEEEMFQFYRNNQQKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPS6</accession>
    <name>A0A8S1NPS6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0820018</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPS6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971">
        <property type="entry name" value="KINESIN-RELATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8088664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="15169" checksum="AEEEEE41763B903B" modified="2022-10-12" version="1">MILDKENLFPGQIEKKINYINQLFRALLINRRRVNEDRSKYEQLISVILEEEENLISTHRSHIDQMVEVVKQEMMLLCNIDQPGSDVVEYVKGFEQIFSTKIEEIQAQLQFQQIQIIFAIINIQISFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUR3</accession>
    <name>A0A8S1NUR3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0980012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NUR3"/>
    <dbReference type="OMA" id="ENDHTAF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="488"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="62957" checksum="CA1E86A73CC83109" modified="2022-10-12" version="1">MLLILFSLVCAKRINGPVHKKLFEQISDTNYVHNIDSTLLLQYNSYPGVYSMELGEVLDGEYRPIKENVTLDPFTLPGCYQYQRRLIHKHLADQQIESDEQTFTDMYIDGDFVFVTRSDNALLKFEIILDLENRKIIGLEFKEKYENGGISQQHQLFCYNKVCIILGEEGQNFIYKDNKTSSINLTSIIGEFREFPKDVYFDEKSKIIYICYENSIVDVLQFTNDKTIEKVKTIIAGEKIVQIRTNPESTILYLLDQQVGLLSYQIVSLLEYKQTSFQIILKNCMAFDFYQNTFFIVAETQDGIPYALEVFSQEDTYYFNKIYSKDMDIYDVWVGEHFAILIGAEIHRVIYHSIYNKFIEQTEIPLFFQDINLVNVEEYRKWDNTSYNDQAIEQEPFQKIPLVYKQSFMVGISNNQISLFSIKSLLPWVQCKPTTTNNTQYSLKMNSTSCLSKQRENDHTAFKQCIILHNFTITGKEIYIYEEDQQKVIILGSILIGTIIILILFAVGVILYGNYKINQYNPIQPDEQELQNVQVGNPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NVF0</accession>
    <name>A0A8S1NVF0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0990068</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NVF0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8095620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="48" mass="5749" checksum="7471F308A7456719" modified="2022-10-12" version="1">MNPEAVNFNIIELIFKHRHNLIIYFLLIKVQKNSGFSRTINISDMMQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P117</accession>
    <name>A0A8S1P117_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1030133</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P117"/>
    <dbReference type="OMA" id="AKLYHWA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18888">
        <property type="entry name" value="NUDIX_ADPRase_Nudt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000152">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF1">
        <property type="entry name" value="ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="42"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8097241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="197" mass="22737" checksum="41D715B1E1F8C70B" modified="2022-10-12" version="1">MDFNTKVDEQLIYEGKWLHFKLLHFQVNGRQMVWEMIERPPGNRGGVDIIPIIKYKDKPSQVIVIANFRPPIRKFCLEFPAGIVDPEGSIQENGLREIKEETGYTAQIGQYQYNGVRIRNDPWKSTEYGLCVIADIDGDSEVNLNPKQHLESDENIIVYKVNMGKTMANEILQLAKEKDYEIAGLLWFFALGQQFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5N4</accession>
    <name>A0A8S1P5N4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fatty acid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1060162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5N4"/>
    <dbReference type="OMA" id="FYLFHNY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03507">
        <property type="entry name" value="Delta12-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32100">
        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="2">
        <location>
            <begin position="61"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8098311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="358" mass="40990" checksum="D61E7C2B2ACD7E88" modified="2022-10-12" version="1">MSKKELEITLGQIRKAIPQHLFQKNELRFLISVIQAISCTLLLTYIAYNYIPLNWFALPIWIGYAFITGTVATGIWVLGHECSHFAFSDTKWLNDALGFLLHQALLVPYFSLQHSHSVHHAKTNHLTQGEAHTPVIIGSKMGKLYQKMKDILGEECFTFIQIFNIAVLGWPLYFLVGVSGGSARGFTSHLIVPNKLLPKKILHKVAVSNIGICLVIYQLYKWYLATSFAEVMALYIGPYLVVNMWLTIITSLQHNDLNIPHYDETAWTWLKGNLCTIDRNYPLWINALHFETGTTHVLHHLFPELPHHNAREANIYLKQILGDLYYKDTKNFWISLYQTASLVGVEHQGNGVWKFVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8B2</accession>
    <name>A0A8S1P8B2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1090045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8B2"/>
    <dbReference type="OMA" id="DIHKQRK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="24279" checksum="9E49F35547DD4A9D" modified="2022-10-12" version="1">MNQLVSIDSTLQANLERLSYLMAQKKEKKPDFRDQFQTNTNTKKIFVNLRAFVKQPQPPSPQSQNQKKEYKIPSQTITTQTQTPKTPTYRQQFIRPQSLAQLEPFIKRQEISVSNIMSERTDGKACYKNWVGLSVIERNDLFLKKKQQKLKKMRDDKDEKELSKCTFTPHFYNQIHIERPSSCFQNKSYQDIHKQRKNQNEKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8N2</accession>
    <name>A0A8S1P8N2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1090155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8N2"/>
    <dbReference type="OMA" id="CQDRSFE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="277" mass="32544" checksum="AD296EEEF8F03D17" modified="2022-10-12" version="1">MSEQQSKGESQENLSLLSSQEEQQKKQVKPTELVNLPKGSSQSLIINEQTSTREERKIKQYLQRIQEQERQEEVIFKKAATLKRKRSKPNPPLKQQDSQEQPISSENKEEEGNQQLALALQLNPLLVPQQSVQQSETSSSQTKLRLNPKLKMKEQHHIYLYLSDCQDRSFEDMQQLQASATHLYSPQKQTKDQTSLNQINQEIFQETSYLIKNIKNQYQQYIEPIQSVNSNDQKEFYNKYFDKKYIESVIRESRLQIHKKSGLEKQTLIGKQIPKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PF83</accession>
    <name>A0A8S1PF83_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1150079</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="6">Part of the multisubunit TRAPP (transport protein particle) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PF83"/>
    <dbReference type="OMA" id="YMVKFDD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990070">
        <property type="term" value="C:TRAPPI protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14943">
        <property type="entry name" value="TRAPPC5_Trs31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016696">
        <property type="entry name" value="TRAPP-I_su5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902">
        <property type="entry name" value="41-2 PROTEIN ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017479">
        <property type="entry name" value="TRAPP_I_complex_Trs31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5 6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR017479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8101373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="180" mass="21419" checksum="E2B84F40E89E174C" modified="2022-10-12" version="1">MKKFQFFDKNIRQQRPELNFCTFNYVFAEYICYLKRKHGNDYNEMNAKMEQLGVSVGIRLYEVVSLRERNKRETKLVEQLRFIQGIFWKHLFGRQAESIEKLKDRPNDYLIRDENPLLLKYISEEGHISPAQFMCGILKGVLNASGFTCQVSYQFKTDERGVSYYPHTVFILSFEDARDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGR8</accession>
    <name>A0A8S1PGR8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1160062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGR8"/>
    <dbReference type="OMA" id="QWINFIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="656" mass="78475" checksum="2C090B1B342667BA" modified="2022-10-12" version="1">MQQRPIINRDLQEKKDKAAQSLHDYKKSLELNYKEYISQTSEQQLNDFESISRPSVKSMPVKTRDLLNKYKAQLEIVPKFQIVSKPNPQLQIKQHQELEEHQQSVSSQCSEPPKNPFEDSRKTKQDEQNISFSRQKEVQLPDSQIKQDIVQIDQFRQKNPFDSSNLMQFTQIQMLDPYAKTISDRNLVSHYTSQESSNIQNLSQTQEKSLDSQQFYDTIEEQGQFQQSGKVNSGTQFQKFDHNQQSSRTYKSIKEEIQDELSSMTHSSHQEEKAHLNQSQIYESLDQESQLVEKAQRWSQYQDIPQNTAQFNPNVQPTILEVKQEDQNSLYIKQDTSNNDINYGQSFDQAQQQSSYLNNQVNSKLLDKVTINDGITMNNHIISKKLSQELVIDMEKFEQNSHLLNSINKTQLLFITEKYFDLMIIKCNKNLLQDFRLLKKLQSYLYKKDIITLRNCMKQFKINKDKQLEKYEKDRISRQFLFQKQQQLKENIFSALQQNKVVQIQFFQNTIQLINKHKLKVLMIRWKTQVYQQKMKKQMDLQYKQFLFNGWLNVAKREKKAKDIKCLTKYYFTTIKKFYQTWKYFTYQQQRLQSKFLNAYRNVNLMKKKFIMLQWINFIQEVKKEKEFESYAKQANEYIPVKVNLKLQNIKIYNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJ35</accession>
    <name>A0A8S1PJ35_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1190068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJ35"/>
    <dbReference type="OMA" id="LANMKCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="182"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="493" mass="58277" checksum="FC7975073F5E70BB" modified="2022-10-12" version="1">MRKKMQKMHRHKLLYEDVLKQVEKEDVAFFSKCTKFRDQPSQSNTLDRALQFLWLKEKVKDAQLKESQKIDRKVALQFGQRRVCKIDSTLKHDGPSSARIIKTIKTDQGMINGWYNGSGDSKFDLGPQYNTKKLEKNNEEIEYEELIKQDQQSPSEYKIKLNHQDIYNNQISEKYSFSNLETKQLLQKLNELKQINKKRNEQRQEYVELEGQRFYHVLNDLQDYLDKKIDNKTLIQEMRAFQTFYPIKRKLQVKQLNPGDSLSQGIESQIDGIQKATFRLFNRTKTIANMKCDLFNTNNYVFNVKEVPNIGQQLRRNHFSQLIEELRENGMKTPREINEVSLENSIKGSQLESSENDVKQIRKFYLKFPKQKGELSQTTSCTKSQRIGTLQSQISNQYLQQPLKTFISNIHGSSTEIKLENPGKFKNRLDSRINKYVKQYIKNQNVIQQNQNVDMTILKEKYKSLKTEREKEITRMYKSQQIEGINNKTVHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PM73</accession>
    <name>A0A8S1PM73_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1220051</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the citrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PM73"/>
    <dbReference type="OMA" id="WVAHWNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032787">
        <property type="term" value="P:monocarboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000002">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.580.10:FF:000005">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024176">
        <property type="entry name" value="Citrate_synthase_bac-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004126">
        <property type="entry name" value="PRK05614.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42871">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42871:SF1">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001369">
        <property type="entry name" value="Citrate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8103823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="470" mass="53570" checksum="E3F502E596A3C1D6" modified="2022-10-12" version="1">MIRIQQLGQQFNATKSTLTIQDSRSGKKYEIPISNNFIKASDLEKIKDENGSIIRSYDPGYMNTVNCTSKISFIDGDNGILEYRGYPIEQLAEKSTYLEVAFLLIYGELPTPLQYDKWCKKIQTHTFVHQDLYGLLSQFRYDAHPMGMVISSLSAVSTLHPEANPALSGQNIYADQKSRNKQIHRMIGSITTITALAYRNRIGRQPNQPHQQLGYVDNFLYMMDRLNESNYTPHPTITKVLDILFILHAEHELNCSTSAVRHLTSSGVDIYTAIAGAAAALYGPKHGGANEAVLRMLEKIGSKQNIPQFIENVKNKKALLFGFGHRVYKSYDPRAKIVKQLAYQVFDLVGKDPLIEIALELENIALNDKYFKDRSLYPNVDFYTGIIYKALGFPTDMFPLLFAIPRISGWLAHWCEFLDDPENKIVRPRQNYQGYKTRDYVPIDQRKQTDAYIHAPSTSFSTRRNVSVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PPR0</accession>
    <name>A0A8S1PPR0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T1260082</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PPR0"/>
    <dbReference type="OMA" id="QINSEYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021319">
        <property type="entry name" value="DUF2921"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF185">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RHF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11145">
        <property type="entry name" value="DUF2921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="312"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8105155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="375" mass="44707" checksum="01AF7A887D791DAD" modified="2022-10-12" version="1">MKNKSSDNLKLLIQTKKHCHGMEKQILWLSKISCEVEYTIQLNYHQYEADQKKIDFTLKLQNEKSYDKKCEIQINSEYQNYLSSIQEQILLQIERDYENSIAQRISILCLGFVSIFNSFIAFENIRLPYTGDYFEYVILLAFFFFCLIFSINVSLFWVISRKRVQSQNLSLIKFFIKYVLIYCISFYNLFDLLQKYKQQNSFLLMIGFFMTPQIIHNIRLGTNPKFIPEYIFGLLSINIAAPVYFKGFTFNFGTSKLQLEFCICWVLIYLVQIFILYIQYKKGPRKIIPRCLLPKQYNYYQDYRPQQLEEACTICLLHLMIEPDQQEQDLDRLQFSRKLMITPCGHIFHPSCLQSRMEIKLFCPTCGNAIPPFYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRQ8</accession>
    <name>A0A8S1PRQ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1280066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRQ8"/>
    <dbReference type="OMA" id="CNQRNSL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="96" mass="11310" checksum="D25576034EA47DF4" modified="2022-10-12" version="1">MQSPQFQKSYENSSKSEQFTYGWLPIKNDVEQSIISYLDQLGINQLTQVEKHCNQRNSLYSLDAALTPPKQQRRTSLKMDSELFQRRSFRMCSDQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSH1</accession>
    <name>A0A8S1PSH1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1290006</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8105944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSH1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="209"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="251"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="299"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="334"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="382"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="403"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="424"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="466"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="508"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="550"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="560"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="592"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="634"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="676"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="718"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="760"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="802"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8105944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="924" mass="102368" checksum="B5595C214FA09898" modified="2022-10-12" version="1">MKSTNQNKGDFFEILAKIKDIDDKILDLIIEIFRKEKISDCLEFLQNVDIQKFQSLYIDKNKEINVENNDNQKITCAIQQIKIHDFNLQNYSSDIYKESKQYIIKKISKEKKIIEFLKFLIRLTTFDTQFIQCGSNSLHLLVEMKVDLTNQCFENIKIKNTYLVGANFFGCNLSGSELDNVDISGINLNGAQLFNCQWKNLKLHELLRLDGHISSVNSICFSPDGTVIASGSDDKSICLWNYKTGKQISKLFGHKLSVNSVCFSPDSTVLASGSDDKSIIIWNFKTRQQICKLCVQSKINSISYSPDGATLANGCSDKTIGFWDVKTRKKKFLISGHSNQVLSVCFSPDGSTLASSIGSSYSNADNTISLWNVKTGQLISKLNGHDYQVNAVSFSPDGTILASCSSDCSIYQWDIKAGLDLARLYGHSKEIKTVCFQPDGTTLASGSDDQSIILWDVKTGQQIVKLIGHTKVVNSVCFSPDGTIIASGSQDSYIHLWDVQEGKQKRKLDGHSEWVESVTFSPDGNKLASSSTDNSICLWDAKTGQLITIFEGHGKGVNSISISPDGTKLASGSDDKSIIIWDVNSGQQIIKLEGHNSGINSVFISPDGITLASGSDDKSIIIWDVNSGQQIIKLEGHRRFVNSVSISSDGITLASGSSDKSVILWNCKTGQQKFKLDGHSDYVRSVCFSPDGTILASGSSDYSIRLWNVETGQQIAKLDGHLFVVTSVCFSPDGTRLASGSRDYSIRLWNVETGQQIAKLDGHLFVVTSVCFSPDGTRLASGSLDNSIRLWNVQAAQQMSKLKGHRNNPTSLCFYVDRTKLEFFFSDNSICLWDEKTGKEIKSNEKRYADVLAQFKTTYQNLSFSEVTNNQTVLLISQQPIFQAQGSLIMKGEFMNYQGVNLLQLFKSKGSIILESSTEFNLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PT21</accession>
    <name>A0A8S1PT21_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1300035</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PT21"/>
    <dbReference type="OMA" id="QICDAKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46344:SF27">
        <property type="entry name" value="KELCH REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46344">
        <property type="entry name" value="OS02G0202900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8106266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="635" mass="73504" checksum="B375FF147BC1D8BE" modified="2022-10-12" version="1">MNRSQSPINNSRNHSASKRYVSPNERQNTQENEQNHKKLPPTQQVKSKIINIDQQSDISSVLGEVNINNTSKMSNMTQFANFMYVPCPTHPEFFITNLCQDQNCIEPLCPECINEHLQMHQQKGRVPKLENIQRVRKDNTYKIDELSKSLQFKLLDSKKYFQEQPSIIYNNNLGQLRQIHEQISNIIDDYFETLYKDLKNQNQDEYLKQLNQIEQDIIQQQHKLTKLQEDLHNDNYVRAVIQICDSKQELFSDKSLIQLNNLIENYQQSQVKIIFDKNNIELFQTYCNKICYFVKNTNTQLNNKSLQIENKSNQSTIKLTHISEIPPPLINPITLISDSLTNDEAKYQINQQNHFEDDKPQVLVRLEEGGYAAYIYDIQSQKYRIETINSQTKIPLFHRLYTTTSGKHLVIGGVDRDKSRFKAIASVYEFNHTTLQLSLHSEMVLPRSMTSACQIDNFIFVVGGSSTNDENTSIAKAEKLDLNSKRWQTINDPYFKCSGCALVAIDKNTLFKIGGKCDIFTPCNSIESYDILKNSWTKIEFKFSSNGYLRLPFNSCAIKISYDQILILGGSVHDVKSNETQILNLKTKTLQKSKELEKSIEFNTQSAIVQFNNIYILTENEQPLLIGNQNGFKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PX42</accession>
    <name>A0A8S1PX42_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NAD(P)(+)--arginine ADP-ribosyltransferase</fullName>
            <ecNumber evidence="10">2.4.2.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Mono(ADP-ribosyl)transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1350008</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-arginyl-[protein] + NAD(+) = N(omega)-(ADP-D-ribosyl)-L-arginyl-[protein] + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19149"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:15087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:142554"/>
            <dbReference type="EC" id="2.4.2.31"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Arg-specific ADP-ribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.31" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PX42"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106274">
        <property type="term" value="F:NAD+-protein-arginine ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050999">
        <property type="entry name" value="ADP-ribosyltransferase_ARG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000768">
        <property type="entry name" value="ART"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10339">
        <property type="entry name" value="ADP-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10339:SF25">
        <property type="entry name" value="SECRETED EXOENZYME S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01129">
        <property type="entry name" value="ART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0800">Toxin</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0843">Virulence</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8107787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="308" mass="36653" checksum="254DF9A5DF240B88" modified="2022-10-12" version="1">MHLSFNVDQYLTNTIEQINNDQQKQGIYQKYKIHNLIALKLWTSNLLYRQLNCDLRNNSYQQWKKYLKCLIEEERVLEEQKIFQDAKTYERDKTIQWRDIQAISLKEKVGQSFSNKEGMIFQIEMISSKDISQISVYPNEQEVIMMPFSTYRVLNVKQDDNQPIIVSMREIPFAKNTNNIVLWVDDNPQNNYQIAKKCETQNNKLSIIFCTSTNDAIQMINSHKWLLLLKKSQLRIITDMVRIENFIINYTAGVDLLNLLHKELNYNGEVLIFTQDEKQAIKNLKQTSYHNYQVTRNQSVLENFIRFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2B8</accession>
    <name>A0A8S1Q2B8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1390034</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8108999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2B8"/>
    <dbReference type="OMA" id="FNHKFTT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001072">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8108999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="218" mass="24615" checksum="2A066680E02E1B1D" modified="2022-10-12" version="1">MSTYKYLFKFIIIGDSYVGKSCLVQQFLHDRPRQEHEATVGIEFGVKDINLSGTIIRLSIWDTAGQETFRSLIRAYYRSAIGVFLCYDCSSMQSFNGVEKWVQEVNDNGNENVVIALVCNKIDLQDRQVSSIQGRQYAVDHGFLYAEVSALTGVGVQQIFRDLSLEIIKRIDNSVIVLGKHLQGIVVGEPNLSPTKQIISLELDETQQQKNQKKKTCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q6G6</accession>
    <name>A0A8S1Q6G6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T1440025</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Late endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q6G6"/>
    <dbReference type="OMA" id="MSAMDMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090385">
        <property type="term" value="P:phagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF13">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08477">
        <property type="entry name" value="Roc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8110391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="277" mass="31937" checksum="448D732991EAE411" modified="2022-10-12" version="1">MEQDSVSILCEQKERKIKVILIGDQSVGKSKLMECLLNIESKVNDKQIQSVDFFISRIMLPNKITQKIHVWITSGEIKNIAMIRAFLLQSNIYIIAFSLNNMVTLENALNTWFQLCVKNAKQTNAYYYFLGTMGDLERKCPKYNEIINQINEKCSEYKVAVFGSKNAHPISGIRQSEKWNDDHIIEFATKRLPQNVYYSECSATENLGMDRSMSIFTKLIMSAMDIQDKLNGIVKVTEQPTITQPQLQSTQDKRPSAKIEIRQQPQEQQEKMCCIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7V0</accession>
    <name>A0A8S1Q7V0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1470006</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="EDSFSIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="988"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1048"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1083"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1157"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1188"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1217"/>
            <end position="1235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8111245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1284" mass="149338" checksum="D9C1169567C4ABCD" modified="2022-10-12" version="1">MKVDVSGVPFNPEQPNLGLNIIIQRYFTFPPHNQLFIDFTIILTNSFDLVVQTDNKKYYQFAQFGALNTEISVKFSFIHLNDMDIITILAEQNASQPNSFEWGLKEIQIYVEECPATCKYCPDIYGIDTCAQIQPLFPHTGTKFDETNNWKISQTSSGNMQLYLSQCIKFIIIEYPLYYKYFNPTTTITSQRTVDIPSNTGTIIQFRIFFYQYKPNSIKIIINNQSYFQNINQVMKFLIGSYNNPTQNINFDNNYYHYIYYISISNYDYNIQIKNIQVEVQFPGSNNLYFSIQGFNIYYKKLESYWLEQIQGCFTMLFNKCVECQEGWYFRSDEQICVPICGDMIIQGQEDCDDGNDEPSDGCFNCKFQCASNCKVCIFGECLQKKELKTEIVNIEWDCQRVGYISRIGYYYDIQQDDCQPICGDGIVTMDEDCDDQNDTPFDGCFNCQFSCPTNCLICEYGKCIYCRVGYDLQRDRCQTICGDSYILEQETCDDGNDLQFDGCHYCENSCQLECTICLNAQCYQCIDGWTLEQGRCEQVCGDLKVALFSYEQCDDPLDINCVNCLYQCTEDCFKCDAPLSCNLCQANYQLIDGKCKSVCGDMLVNPLYEECDDGNDIPFDGCQYCLLQCSYGCLICEENNVCKECDSTNYFLNSTNSLCQEIPLEIIEQEEEEEEEEIKIEDVQCNDNFILINNQCESLCGNGMLASLYEECDDNNKNSGDGCSSQCKLEESFICLNTEDSFSICSFIILPDFKLKSLSDVKDQTQIIELQFTEKVKMFQDTILEDICKFTITPQTEYEMKIIPITNLTTMLSNPKYQFKILFLQPVQNPIFSISIAKQQIFNINEIELKILQKDISLGSPFVMSKESKEFVTSVVKMNDAMMYTTIAISGFALLTGTPAMLFNLIDLLQQLSYIRFMQYKFPSHLRQFLESYTKISLKPIFDYFQFDQFLANLNDGQLPLQPRKTISSNQADPLNSFYIINAKSCYFSLFASFFSYILCLTISKTSAIKYFNKIIKNKGMKLKNLTKTFQLYHNLQVACIKFRNKYFYSGLFQVYYTIMHQFLFSAFLQFPQYNFTSSLEIFNSVNAILALGFILFIKFQQFKITTGTIKNINDWNFFFDGINKRFWCQIFKPIQMLKITSYMFAIVFLMDYPEAQSFILSAQSSFYVVYLIIFKPIDVGFEYIKLVSREVLFISTICSFYVYSYELDDDKMFQLGWMHISLFTSILGSNLILDIGKYIKLAYDNIQIKKLKVERRLNYQYQNNQLHQFIMVEYEQHPKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9K8</accession>
    <name>A0A8S1Q9K8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1470092</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NGDISCY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035919128" evidence="2">
        <location>
            <begin position="25"/>
            <end position="3043"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2581"/>
            <end position="2606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2627"/>
            <end position="2649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2690"/>
            <end position="2712"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2719"/>
            <end position="2737"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2776"/>
            <end position="2801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2887"/>
            <end position="2906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2918"/>
            <end position="2938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8111391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3043" mass="353271" checksum="1711BA3C998E60C3" modified="2022-10-12" version="1">MIQQISIQFCIALLLLQQLITVECISTCPFTNYKDALLIMETSTQSYTESINDNDNQNTLGFAFWSISIPLLEKLETVNRDEPFKNDESDNGELLFLVKDSILSQNFIYGFKSYDYENRQVKHIIKFMNQDISKTLIFSFGSLEYEGIWIFHLILLQPSLNLIIVEVSSQEKQSTNFDLQNQLNLQISIGGRGYIDNLNLNSFRGKFSKQIYLSKFVYSTNTFTQQISDCKIPQKIIGEQTINIITGLKIFEGNQVLQQFIDHYGNRYCLQGWVKYTLPKINENRYTLLKISGQYNYEQEIMLGDELFRIEVFISKINPEQTSLIINVDAYGMPVKQSFQSQYDLLFQGTVNADYSVLQVKRIYQDLNTQLYFEGLQQWHYIQYEYGRNNIEERMFLQIKFSNDLGILQQNFGNDIFSGSFTNSRFNVFFGGDIVNPNTNQQFLEALIYDFKIQYNYEEDKLFNMKCHYTCFTCNGPLDQNCQTCDPNSNRFYQDELKMCKCNSGYFDKGTSICDNKFYSSIQLEELPIDSEVICPIGNFRLPRDDGNGYDCLECPQFSNFKAVLCANCYFYPFTWYLEPVCNMDYYTIKQVVYEQEAYHLIQRTEIQNDVYSIESDRALHLNPEYAYYCQEGQCNEFSKSHLGKTIWAECKPNYYYKDYDNDYGECINPGLNCLKVNILKSCDICPIGTYNNYYTGCDACPLNCYACTNTFSFILTCDACIQSYALQNGVCKQCGLRCDVCKDYYDQNLGYNYLKCLKCIDNSQYSISLDGINCISNVIPHCVHSFQTLIDDYKINTLDINFIPQFDLNKIKLLCAKCEPNYVFVFETQSCVYNNINTQCDIGIGSLNINDQSLETVICLKSYYYENQIVEFMDKCDQQIKNCQVCLETNIQYYYTCLECQNGYYAQQVTGQCIQCPIQLNCISCYSQHSIIKDHWKKNIRAFYKKYIEIKNTHQFILNAQSQNVNDYEILCSTCQNGYRLHKNICIKYCSDTCVECILKDDQYYCVKCQNDQKGRKQSTYNNECIECPEDCALCRPRTQDEIKYINPLFNNTKYTKYTNQCILSFQDQQYNYDEDIGVFIKCEQSQLNACYKQLVINLNAYDNLDEYNKDYNKLIDEESRLKFKRENFHMHRLFEYDNIFLEYQNDDFYSLANANHIKSILIKISNSKPINYLVYYLGNGGIKQMFSQNIFSLINVEIELNFPENSSLILQRNLEITFLNFNKITIQNLKIIGCFQCTNEKFIFTSLFPQQVILNQLYIDHITQGSFSSFVFIFQNVSILHIDDLTLNTVINYDINKIIDVQSTPFNKQIIFKNFKTINCILNNHIFFNLNLRNKDTVSFENFEIAGIYKLTNLIIINSEDQKSGLINIKNIIINANITDSFAFIKLEQVRSLSISDFLLNTTVIGNSSLLLLNDIANLDNLNFTNNQILDNSVFILNFDQKDTQIQFNYKINNIKFENNQYNSVIKFINFQKYSSLEQFIEINQLSQINNVLIDAKLQYNFKQQDSSLILLLFNEIYINNFFINRGFGLNDITISESSKLQFQNGIIIQDKFTFLGLHKHLTCQLLYVDGQYYSISLNILSSKDIMISNVTFSKVQSYNFPLILIISTNIMQEIQDSNIILKDITFQESLVLLSNPVFQTSIITIQSSQQTIIQIYNITFKNNLLHVYVEDGLVIAAGLLLIDCPSSQINIKYSTFQNNLVTNSSTSIIYIKSQLLEINNCLFENNGVFNYTVLQPHIIWAFTKIVTQKTINKIFKVISTAGNGQILVEQLRIHNSSFYNSIGKLGGCFQIFTLRSSTIEIINNVFYNFSTQFISEIEQGGVLYIDGSSSSFLDIVITNVTVQNVYCRQYGGFVYLKSNNSQTHLTILNGLLHDIYAQQGSVIFVSYSNLVEEPQTVNTKELVIINSQRGYINYLNRFNDLSNVQETSSLINNRSSIYVEFGSKILIQNISANTLMFESFLNFEQSNFIYISDILVENCQLSNNLIKMSENQNVNLIRSQFRSIIVGFEIESVNCQIIPEINEIINLECLEGFENAPIYLENDVIDESIDRGLCVSQIIKSKLDLSNSGLIMINDIEYQDFAKISQFDLSNISCANCANGLLYMQFLNETKLQQTQIVQYPKVVNSICGNKGCLIFEKLLTIQNGRILTEKQDSQEGYELQISYYICENNIGFTGTCIMLNSIFTSIQNSVLQHNNATFQGGAIVVKGSDQVIIENSLIQYNQAEVGGGLYLENTFALNYELLKTRVILNTAIFYGNNIASIPEKLAIQITQDEFLQTKVLFENPKKKIEEVIIKPFKSINGKNSKFVQLPTGQQISTYQFFDWKTQNYINYDLIFRIHNLNRKMNLIQNLSNSFCTINSRRYNILEKDEDQEFTNNYTKYNTIQYNSETQDFNLDDLIVYFDNDVPEEIVLQLEFQCVSIKIPIFNSIYPYQTERFHNDYKLRINIKTLECQFGEIKNLTDFSCVICDSNQGLFSLTLNAQKCDIQDDLSTISVRSNQLQLRPGYWRPYFDTTLISQCLNLASNCLGGWRNGDISCYTGHIGALCEQCDIYDTRGEGSFSVVSRYSCGTCQNKYQNIVLIIGIIIITLIFLVISVKGTLISIDEFSKFKPFFRSTLTKLTPQSSILIKILTNYFQIVATITTFQLELPQGLVSTLDGVGNPIQTATYSLDCFLVNFSDIQIQYTRMIWQIILPILYINLFMGLFFIASKLNLIESMLNCSVMSTTLIYFYIYFQPNLINGFVQLVSYRNISGFQWIKSNVADRYDTPEHFKWMIEFCLPVLIILTILIPFFFFYGLFSNKNSLHSKKEVKMRWAYLYIEYKETVYFWELIKIVEKELIILSLIYYEERIVIKGVLVLLITYFYQELNQNYQPYSMNKMNKLDYYSANICMITVCLAIGAYIAQEADIKELQIPFLVVMALLNFLFLQSILFQIISKYTQQYEDTLDKIKNFIKQKYPQYRSSKYLNQILESRTEQRNRVQKQFNKLKKYAIPLGRQMIQTREKYSKSKQSNAIEFHTRQETIDIEKSSQKRLLIIQNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCM1</accession>
    <name>A0A8S1QCM1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1500065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCM1"/>
    <dbReference type="OMA" id="RQANNTK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="271" mass="32297" checksum="8A1B320523627888" modified="2022-10-12" version="1">MNNVNQFDRSLLTVDIVGAQPKQKSSYLDKIIPQDIRQTNNMKLQYTIPKQDPQYGRRRYQHDFHHFCNSCVPQGENYFDGSHKKHYNLHQGEIKQYRALENYYDQPKYYYIIQGNLQGDRLLATNDIEGAIPGTYTSKVVRNQEKAQKIRQERDYLRAQNNKALYLSQLESNINYQSEFEQQPQRNKSYSIQITPQNQVFENKVTEIPQPKYEYMNKPMKLPPILERETPVINPISNKLQLRQNYKNYLLEDYDKTISMLQQNQPMKLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCV7</accession>
    <name>A0A8S1QCV7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1500115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCV7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="837" mass="97165" checksum="359809A1D5E22FDE" modified="2022-10-12" version="1">MLQDAQSQLNMMQEEINLIFTNVKDVIKSLSDYFSSGDMSFQNFLNKNEKFMKFSTTELNFQKDFLERNKFVEQTKSKEILASLIEKAGIIFSKIIQNAYKANYLIEQIQEYYINEKIEENIEVSEGLLGYRNRLIKKQFNLLTQKNGDQVFIKDGQILGILQKNMGSVTGQVRYNLEKIKYLQFQGQFGIKGYRIKQWIYYWKGLQVGGGYYNLLGQKEGEWVDLTNNYWDFRKIVEKGSYKQGKRVGVWYIHMNNQVMIGGSYDEKGNGLKNGKWTDIADGYQQYQELTYNGEYLNGKKIGKWVIKFRDQTIGGGSYDDKNKGIKTGKWIEISDNFNNLTQILYNGEYINGIKVDRWDSFINGQFKDQKQYKFGGGSYDNKCWGLKTGYWVEKLDGYSLYDNLISNGQYKIGKKFGKWEIRLKNEVIGGGFYDSRNFGIKVGNWVETNNQYSHISEITYLGEYWNGRKVGRWKTNYQKNQVGGGSFDQGGNEVKIGKWIEIHDGYRWDSYVTSNGEYKNGKKFGFWDIKFMNKSIGGGLYHEGIKNGMWVDIVERFCDDLQITYSGMYKQDRKVGKWDTKFRNYYLYPFQLIGTGQYDEEGKGMKIGNWVEISDNYERGQEVIYSGKYENGKKVGNWDILFHQFETMKIGGGRYDEGGNGIKIGNWIDISDTYGTGYNKSQVIYHGEYSNGKKVGRWDIKYRKYNNDSFLTMGGGCYDEEGQGLKNGFWIEINDGFQDGNQITFNGEYIQGKKVGRWDIKFSKYISAPFLLIGGGSYDEEGRGLKIGKWIDIQKGISIYNEVIFEGVYKNGIKIGRWLEISLIDNEILSDIQYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFH0</accession>
    <name>A0A8S1QFH0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1550099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFH0"/>
    <dbReference type="OMA" id="HYNRDFL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000051">
        <property type="entry name" value="Protein phosphatase 2C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="186"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8113517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="467" mass="53150" checksum="06B0F4404A27469D" modified="2022-10-12" version="1">MKRTVDIYKANGKKPKQAIGSKSQTSYAAYLNSDVKTIAKLKENLTQITNNYFIKNLRIEQTQKSSAKQLKTNFSQLRQPRSATIDTKNSQKKISRPYESLQPKRQTAVESTQNLIKQYTNQKQKTNVDKRNLWHNFLNNSIIDQQVRRAQSSSTHNMRQEPKSDLKYRSAQSLHYNPDFLQFKLSFSARTRQGQLASNPNKTNQDTFICETNIVSDMHLFSVCDGHGQNGHFVSQYVRDQFTKILKRDHQLKQHPRQAIVKSISLLANLINQQPFDTQFSGSTMNTILIQDGGHLICSNVGDSRAIIGKLGNNSKFKPFPLSIDHKPCLEKEMNRIHMHGGRVDTYYDDQGNSIGPARVWVRDGNYPGLAMSRSLGDQIAQSVGVSSVPEIFEYQLTPQDKFIILGSDGVWEFIDNQSVVDIVGKHYVQGDLEGACDELMQISYKMWTLEDDSVVDDITFIVIFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QHY3</accession>
    <name>A0A8S1QHY3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1600102</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QHY3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="417"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="623"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="882"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8114621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="968" mass="116410" checksum="E99A7AB726EB3532" modified="2022-10-12" version="1">MINPNVGNSIFIEGNMFTRLQQTNMQGPQLNNDIVHLIGNPKQLFILKQYNKIRTNEIQILRLIKSLQNKEQPECQNIIKIIGIQEKQIQNQLSLQVILEKSEGNCENLISANTISLFQKLNIFEQMVKGVIQLQELSCYLKDLKPENFVYFQDNNNNYVIKLIEFGFLNQESNPISQIKSLKYIAPEILITQGNYNINTTSIWSLGIILYQIVSSQHFFQFMNQQELIQQINSLTQEQINNKINQILNIGQQEKDLITSMLQKESQHRLKLQSVLEQMQIFLSKIQEQEETKQLQFAKDQYIIFIKNVKHNYNLQVPQIKLISEQFKFYQLIYQQFQGQDQISYLIKFNQEISETIQQIEQKESSNQQFDSRQHEITVYKEFNEKLKQIKYNFDNSFRNLIRDANQFIQRIDKQYMESQNEQIRRYKEQLTKQQEKFKQFSNLPTYANIINQVYQKIEEYFVQITKLEQLIIQRTQEAFSEYKQACQQIHPQLVDFDKENDEIVKKIQLSIQIEKKKEEWRKNQNNVQEQWKILVENTPKLKQQMIQLINNPICQSLKKVIDEKILKLDQDISENENLLNSYKNFNQFNSVEVINWQIQILESFQKKLKELYLNEKIQVGQLQSYSNNVQELQKKYKLFQQATQSINSKFPNYQYFIKIHQENEKHHQQLEQEIKVLQEYLMHQNENEQLFIQALENMKTKTQSQKKQEIEKRISKNQEINKQQGQILSAVLQKVSKLQSELKFTAQDHNLIMDQEQLNVLNNALQNEINMMNQKISNQIFSIQQLTQQTIEISKLNSKIDDQMKRIPQKQIVEDFINKFQQNQDSYEKQYALVNYIKLYHLIRYINRIKQKPNIMDQIEITKYEKETKSLLQNYENYLFNNKIQIKIEEMEINRKELEQQLEKLEKILEQQNYDSLSERLKFEKQIIETKWNNQFYKITDFTQMIRLNKIKTEIQQVIQEKFRNQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QIE3</accession>
    <name>A0A8S1QIE3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1660046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000175">
        <property type="protein sequence ID" value="CAD8115798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QIE3"/>
    <dbReference type="OMA" id="CTNKFRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="58"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="245"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="472"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="653"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="776"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8115798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="872" mass="102184" checksum="84A8DD2E1EF78885" modified="2022-10-12" version="1">MSLSQAGRQAINPITGESRFQAPDYRRSQEVKNEPTRTAESRRNFANTSVPVENQEQSQFQMDRIAQLQDQVQMLEKQLTQEVKRNSKMLVDLEREVRTRGQMGDPNQMFEDLQNKMYSLETKIIAQDRQKSELGSKVAIQEQNLKELLYFLKNTQNKDTNEVLQMRGMLQEKITEESSHLQKEREKTKALFMEMVRLGELQEKLQESLSTSHQHFEQRINGMESKIYNGEKALIQVAQRGDSGMNFINETNERIEKRVMALEANLLALGKDQLKDHEQINRVDMANQRIQEEFKGMLQLIQQDYTQRLDTRVTEVINRIVMEHEQRVKTIEEMKQAWSLKDQINMERLQYEREEVIQKLGSLEQFQRIDSQKKDEAIRSLQTIIETQVNQILVNIQNEEAQRYSHEVQLRGDLLKIQENIKQENELFKTHQANITEKITDMIRIEVQTRLSTDTELKNLTSAIASDIVSDLNSLKDQIEMANRALQTQVKQLEKDQAEKAERLSLYIDDEINKAIKTSAQKYEKVKVIFSKFGESFKQHITAYEGLKQDLNSRQTIIEQNIDMIRQDFQTVIEDQQTHLLERISIEKNQILEAVNATVQFLEDKVNKLDEDCSNKFRIFREVIEENQQVVVEKTKLILDQNESYQQTQMKGLKLMAESIQELKQQQQLIKEDHQNQIKEVNQVVENTKQDIQQINTNIKEINDIQVQHKSLLDENQTDIEILKKENETIMIKQQDIDKNVELLNEEQKKIHNTFQIITQNVEQVNDKTLTIGERVDAINSNFQQLQEELKLIDQKNSKLEGDLEDSNLKINNEIKNIQTRLEESQKTAASIQDSIRDNDDIDKKQNLEIDELQKSVKNLQDRLKQLAQLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QL48</accession>
    <name>A0A8S1QL48_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1630095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000170">
        <property type="protein sequence ID" value="CAD8115320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QL48"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8115320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="43" mass="5133" checksum="0424A5F2EB933452" modified="2022-10-12" version="1">MFICIQTSLINVISQGDSLFCLQKIQKKVEQYICQKLKYFFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQ75</accession>
    <name>A0A8S1QQ75_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1850006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000194">
        <property type="protein sequence ID" value="CAD8116955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQ75"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="712" mass="84417" checksum="0C8139542A400914" modified="2022-10-12" version="1">MQQNLVYCLNHPNSLVTHICTGLHICQRKLCPVCQYDHQIIITGTQILPLETFKNNLQNNIEQFQLNDSIRLDRAKQEFKKAIVQIEQTFFSLIKEMIESSNQIFQEIEKIDKYYLDLLNSNIEESSNSKLYKISEILQLNHLNLWQQQKQYCYSKLYENKHNLIQICESAAIKMREKIKTTNSEKIGLNVFQNSKLLGINHCDFIYINNEIISKQGNMKLNQRTGVFDILGFSQYENTLKAFQIEFQQGKLCYIADGKILRIEINNDQNSIKEIITNYDQLCNLKWIREKNQTQYQIELWEAQWGKQHLGLEGFYDKQGRKQYFWIEPCSNYSIQLQVVYAGYYKNGKKQGKWKLLKKNSNEFVTIGGGNYNEFDVKEGQWIELCDEILPQQNQIIIDIGKYNDGHKMGTWKKYIYEESIFTKNKLMLVFSYFQWWRRISFWNQKWEMERDSLQFQRNFKSFWLVLIKMDQRLVNGIFIQSKIMNNNQLCKALIFLCSGGGTYNNLGQKNGLWSELSNCFHQSHFTIFNGKYQNGIKQERWMIQHKMKQSNSIEILGGGEYDEQGLRNGDWIEIFQNYDKYSQITFKGRYLNGLKQGQWDILYSNNKIGGGNYNKDGEKQGKWTEVHENFNIQTCNILYVGMYMQGKKLGQWEITIQSREEQKQKICGSFEQNGLKSGQWIEINQKYQEYDVIYQNGIQKEDNEQNQDIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QTH1</accession>
    <name>A0A8S1QTH1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2760003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000285">
        <property type="protein sequence ID" value="CAD8118979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="493" mass="58527" checksum="768C2B434493AF74" modified="2022-10-12" version="1" fragment="single">MGKKGKIQLNQKQTDHIQSFLENDEFISLKSNDALRNLSINLQKLWSVEKYSNMMRQIYLNKLVVTPYMMEIVVQVLPEMIIKETAINNLKQNCINIFSQKLKALYISKYLIEMYQQQLNKLVTSNNGDETLRKLYCLIQKIQKNQSFQKLLSYFWKKIEQNPILKQTQQCSQENTELKAQLLQIIYQIKLQFKKKKKREAVIEAVIDALKELNLSSYDFYDEFINQHHQKQIEKQRKLGKSINIDLFLHDLKQYSTNLAKAMSMNQMTQVQYQQQGLLYKEEKEKEKWLNDFFNDDDQFGSYRKDIRSCSLVQQNGANFQFVHKSIQEFLIAADLYEVLVQSKDFDIQILNILQQNCCKKKQSRIGLLKFSIKCIRNKFQKLQFTRKYITYGKTNTVNSITRLIKTIKKHEISFLNYSTEIYAETRQYLIQKISQETRIIEFLKFLVHLTKIDYTLIICGSNAINILVEMKVDLTNQNFQNIRIQNTSLFXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQA4</accession>
    <name>A0A8S1JQA4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0080289</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQA4"/>
    <dbReference type="OMA" id="RENYYHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF3">
        <property type="entry name" value="FERROPTOSIS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="7"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8044723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="393" mass="44982" checksum="233F69F686C142DE" modified="2022-10-12" version="1">MRNSKKTLLIVGGGFAGMTIITQTYKQFNIILLDQKSYFEFVPSVFNAFIHPESIFDLTLQFKQSKFGVLFIQGKLTHIEDNIAYYEGGQIEFDYCAITIGSNYTNPIKSTIPKLSDRFIELKKAQLKIIESQTILIIGGGTVGIELACEIKASYKQKTVALITKGKILSTMPKSASEYAKKRMLNLGIEIQENYIGPSLDSNFDLVFNCKGNNYESVRLNNNFEMFDPKKQLLVDDYLKIRTNQNIYCAGDICISSQNETKTAFAAEMQGEIIAYNLKHPNKQIKSYWIPNTYIISLGGWKAVFVFETFTFGGFIPYLMKLFIEVVVVNDFRGIIGFNTIHQIMNYIVYVMLYIYMIMQLLFAIAPLGSKIKQDQRVELKRIQQEIEEFKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRG0</accession>
    <name>A0A8S1JRG0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080169</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0080170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRG0"/>
    <dbReference type="OMA" id="PDECISV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036273288" evidence="1">
        <location>
            <begin position="16"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="225" mass="25091" checksum="0F6FFB21B6D42EE4" modified="2022-10-12" version="1">MKLFIVIFLIVATSAISINSKCTAEVTKLTPRFSQLYGNVNEKKFVSSIKELIGILKSLVAVKKSCTGSEGVDLSNFKQKIGVCLKGGFELASNGYKSYNEFEEDGFSDILVDSLVEMSHLVEPVITNCWGEEVKSVLGFVLPDQCISVIDDVAKVSLQIIEQKERPLLVTKGLINLRKIFKESKKACPFVETLIPKKQKNDDDNEDSDFSDDDDDMEALFELFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSJ5</accession>
    <name>A0A8S1JSJ5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Non-specific serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0090038</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSJ5"/>
    <dbReference type="OMA" id="HPFFIEP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001236">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033699">
        <property type="entry name" value="POLO_box_Plk4_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51984">
        <property type="entry name" value="CPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="9"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Cryptic POLO box 1 (CPB1)" evidence="8">
        <location>
            <begin position="388"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8045015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="65671" checksum="4D743D484B4D5302" modified="2022-10-12" version="1">MKSDSLANYQIKNPIGRGAGSFVCKIVNRLNKVEMAAKIIQKTSSNAQKIQNEVDIHLELHHKNVVHLTDIFEDDDNSYLIMELCDSDIYQLIKKEQFSEQQIRYYGKQLAEGLQYLHSHNIIHRDIKLGNLLIQNDILKIADFGLAVKLNNDEEERSTLCGTPNYISPEILNQQPYGKKVDLWSMGCCLYAMATGHGPFEEKNTPLGEVLRRVKVGDFDLPNNFSEVFKDLIINLLNLDADQRYSIEKIIKHPFFIEPIPPRIQSRNQSSSQIKQLLDLSPFVKHQHRQTCSMIGLEKQQIVASFLQKQQQLGGKKLLFGMTTNCKGGLKQNYHLENVPRFFLPQESHSNNTSCNHHNKENINKENCNKENIRENSVKLKKQNSSTINIYNESPIKLEDLKSCKLQTKNGLLQIHEDGRFEMDVCNKDLNFIIQTNGQEILVQKKGQKPKQYRLNELPQKLQKFYTYSKQVCNAIRERNQKHKITNEHGNFALKNTKLGQCFEGYIAQSKIKIQYILNSDTLKLQLQNGTLKSININEFQQMSTQARMDPNEVYSIKIALKYLTQCQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTC8</accession>
    <name>A0A8S1JTC8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTC8"/>
    <dbReference type="OMA" id="DTHSKCT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="214" mass="25479" checksum="E9B3FBAE8ED21483" modified="2022-10-12" version="1">MRTQNNPLIHNPTLHTISNQEDSFYENNLYYLNRASQERVYQPFTQRINDKTKNQQRNKYLDPENKKMEFFEQLQKHQLYQAKLDKTVQYYSSQKKDSFQQSVNSSKGQFKIASLKVSERNRNLSERNDYTHQLVKEHNIIDDIVQNQDLITKQKGLLEKVQDLKKLKLLKQYKFNHYCLPGVGKSPYVFNDTHSKCTNPGYSRNIEGGKFFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWZ1</accession>
    <name>A0A8S1JWZ1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090176</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWZ1"/>
    <dbReference type="OMA" id="NGFENMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="220" mass="26363" checksum="34226A8F463BE413" modified="2022-10-12" version="1">MLTQIEDKISTILNVGFKEQLAKFKNQSFRKRQSFKETELRRKSTDYWLASDKEKRLDLFMPIDQHQQLNNKLLLRCNTKTLLVKRTKGLIRNEVKSKSFQSINNTVQNKIFYMKKSAQHLDKQTDFETPSKRINGFENMIKSKNYEIYIRGRTNCMIRNYINEIFNCEQKPDINNISKRIQTLPNTQIKKQKNQLDRNEQILRTYNSKQNDTVLKSLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZT8</accession>
    <name>A0A8S1JZT8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">CLAC channel</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0130013</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMCO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZT8"/>
    <dbReference type="OMA" id="CSISIRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008559">
        <property type="entry name" value="TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20917:SF0">
        <property type="entry name" value="CALCIUM LOAD-ACTIVATED CALCIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20917">
        <property type="entry name" value="PNAS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="10 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="36"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8048634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="20681" checksum="D3FC292F48457272" modified="2022-10-12" version="1">MDINIGETLFIIVGAAISISVGELVSWFLVYRNADYKDLVSRIETAVAKYNKEKEQFVKETNAKNQEKRLSQIESQIKGLNYEMTFKKMISNAAVAILSIVTINSIGNYYSGIVVAKLPFEPFWILQQITHRGLNGEDLTDCSYIFIYILSALIFKSNIQKIFGLEGPKMPFGPGGPQPSLFKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1R6</accession>
    <name>A0A8S1K1R6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0130061</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the metallo-beta-lactamase superfamily. cNMP phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1R6"/>
    <dbReference type="OMA" id="LEMIMQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd07738">
        <property type="entry name" value="DdPDE5-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000029">
        <property type="entry name" value="Cyclic nucleotide-binding domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000283">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="10">
        <location>
            <begin position="576"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="10">
        <location>
            <begin position="711"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8048746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="828" mass="94535" checksum="B45A2954D75756C2" modified="2022-10-12" version="1">MACTSKEQFHQDINELNQELKPQEQISTVQTMKLNRGGLIIKTSEGPIQFGLPPETVKDSMSLGVEVPTFYIIPTQRFNKQFGINVAEFEFPAYFNFFIKKRQVTLICDTETANSIKIVFQETLLGPKDFSQIQNDYHPDVDPIEYVDFKKELAIFAKNPMNLNEKLTSETLLKFVIFENNVAQIGKVKIVKEEKEFTIYDNDNLLISFPDKFSFPKEYDKLLSLHKIDSSGKSEITPPSLLIMKKMKHGKKPPSLTQNFDEVDSDEDDQEIGSMPNLLLWNKNGEAKLQKKGSIELNEGLTFQAPEFGVTVLGCSHGFDPKGSTSGYIFWINGRGIMVDPPPYTSYHLKKMGIPPMMISAIIISHCHADHDAGAFHKILDDSKVEIITTRTIMYSFLRKYSAISNLSQEQIKSLFIFRPAILGVPLNIYGAEFEFFYAFHSIPCIGFRVKYQNKSIYFSGDTFYDPVKIEELYKQGHLNQARCRQLNSPKFTEDIILHEAGVPPIHTSQTVLSQLPNQVKNRMYLVHVAAKDVLKGSGLKVAKPGIENTLILIRPNVQQDIPLNRKMDIISRIDIFDKLTLNNAKFLMDALKLEKYKEGELVIQENQEGHKFYIIESGLAKVFSDQKQNKFQRYLQTGDYFGESALIQTGRRKASVLAVTDLRVLSLDKHDFWFIFGDGFEGQGPVIEQMMQLMEARKQQAIQILFKNSHFSQLTPTQKTQLEMIMQQQDISKGQILWKKDDEATFVFFIRKGAFVFIDCDEAKLEEFDSGSFIGEINAILKGEKLTTTVKAVREGSILKIEANAFVKFLQRNPGLLLLLQEYKYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2U1</accession>
    <name>A0A8S1K2U1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Clathrin/coatomer adaptor adaptin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0140065</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2U1"/>
    <dbReference type="OMA" id="HVECSAT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017105">
        <property type="entry name" value="AP3_complex_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22781:SF12">
        <property type="entry name" value="AP-3 COMPLEX SUBUNIT DELTA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22781">
        <property type="entry name" value="DELTA ADAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="8">
        <location>
            <begin position="35"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="652"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8049589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="713" mass="82307" checksum="6891ED6BB10FF45F" modified="2022-10-12" version="1">MFHLSQMDQIFLKSLADVVRTSRTTSQYDKLTFTTQTIADIKNEIQSRQESVRYQALLKLFFLAMEGNNIRWAEFQIINLMGSGDFQLKLGAQLAAHLVIDSQSQGLIMVTNVFQKEFKNGHVECSATLSCLGTIANKDLSDSLLPHVLKLTTNTKPLIRKKAIAVLSKIFTINPLNIPGNLEMVIQQLQKETNISVLACGISLFCSVMKVAPKLYPLFLSIVYDQISKQKSNWLLIKLVRISNKLISLEPRFQGKLIEHYTRLLNQTNSKSLQYELIYSIMKYFKNHSQLYESAGDILKQFLNHQDPNLRCLGLECLTHISSSAGLMEFQEQILESFKKSDYFSKLQILQLFKDFTNQQNFQTVIEFFIKYSDLESNHKIIESLIFIIMKDKFINVDDPEKLLLMYIPVIAQKIDTIDNAIRFKELLFELLTRVPGLKVYSDQLCNQILQTYAIQSDSLQGPTLNIVQRHTQLKYSLNHREIIFKSLIMIVGESVKLLKLEALTNIIKFLEQLNILNCYESSLSDIDSLLFKIYIQIIKTDPSNDLIPQIEQLIKNIYIKLHNISIVKISFFYESLILNKTKDQLDQLYNLYAEDLPPINLEAQKLIKPPEGLDINLSFQIDIEEIEKIKNSNNDQLEQKPIIQQLQQIESNQKEQEDQNTLIQKQGENEEELKPLNSKIDPIPKKIFTINRDEELPPGINQNDINKSKEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K391</accession>
    <name>A0A8S1K391_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0110071</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K391"/>
    <dbReference type="OMA" id="LICQQLM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04515">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052969">
        <property type="entry name" value="Thr-specific_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763">
        <property type="entry name" value="ALPHA-PROTEIN KINASE VWKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763:SF1">
        <property type="entry name" value="DUF659 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="VWFA" evidence="6">
        <location>
            <begin position="493"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-type protein kinase" evidence="7">
        <location>
            <begin position="760"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="698"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="143"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="406"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="710"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8046726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="995" mass="117820" checksum="C32247E8428711A8" modified="2022-10-12" version="1">MQNFNQQGFENQQYANQQKDINQQNYTPEQIQAYYQQSLTPEQLAYLQSIYTPEQLQAYYLQLYQQQLLAQQQQYHQQVQQVQLQNGIQNQINQVPQKIQNNQNHQQQTQQFPQNQVYAYAPNQVYPPNFYIQQQPNNNNERLRQLTSQIRDLETKIQYLENEKDDLENKKTKEIKNYQTQLQYERDKVKQLEEDEKKKDEAILNYKTDQFQTNFKISQLEKQCTKLQDQLREKETEIDKLNSNNQKIQFMTQNTKNTTPDYEKQMQQFCNQQQALLQEEKNKYKLLNNQKNSIEDELKQKDEKIIQLEKQSQDLQIQVEKEHKYFLNAQGEIANWQRKFNFQNKQQEEKDSTIDNLTKQLRDSKFENSQLVNKLKGEEESKIAQQNQFKSIEKTYKNYIEELQKNKSNNQNKSKFEDEISKLKNEMLQNQQKYEEEQKKAELLKEQEYQENKIKKAMQEQQRLENRKQENKKIKEIMMKKTQGSLEECGKLDICYVVDFTLSMQPYVEQARCCVQESLKIIKQQTNRDTNISSIAYYDIEQKPKTGSYFQFDFSNNVQNFQQFMLDIKFEGGRDPPEDVRGALEQMMKNLKWKSKFKIAILITDSPCHGKKYHSFPVDFHSDDDITQTLHRLIEQQIILIGFNLNDKTTKMYEEFKKVYEAKNAQDFFLLVDVAGLKVHQLAEKMASSLGSASVNATQVKQSGTKSKKQQQERPKNKDGAMEALCKQGDFFNFEQQTKVVDTVFTVLNVTINDQVFNNNLQSINNIGKQPNKDYIIKEEGKWSCIRTELPFAFGMMKDVFLMKKKNGGPEELYVIKTPLGSKPYQSQLEAIQECRSHLICQQLMKKFKNDIADALEQQNFQKNEYPNVVYSDFLILEETKNKYWIAERFFKGEFVKYNNNFGYINEEITNLNKFAQAFSYYTFFISKGQYMINDVQGVGNYFTDPAINTTNGDFDDTDLGQEGQQMYLVNFQSKKALAFEILSLLNQLNEDQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K5A6</accession>
    <name>A0A8S1K5A6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090474</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K5A6"/>
    <dbReference type="OMA" id="MENNQQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="169" mass="20431" checksum="12A3543A034A1524" modified="2022-10-12" version="1">MCENYCRMQLLYQLRQRKLMENNQQINIYYRIKKRVQNSKIQEQELSFSESIEKESIFPFIIRKSPQEHKDQIQSINTGNNRSVPQNKVKLNAKPINNNRSISVSRRISPFYDKNLIYDQINTKLDKEKKTIVVFSQRENPLKNDYIYRFCKRDSQSSQSRRSNYNAYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7X2</accession>
    <name>A0A8S1K7X2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0100504</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7X2"/>
    <dbReference type="OMA" id="ETIWCIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053083">
        <property type="entry name" value="TF_kinase-domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305:SF2">
        <property type="entry name" value="SI:DKEY-192D15.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305">
        <property type="entry name" value="SI:DKEY-192D15.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="358"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8046608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="492" mass="58588" checksum="ECE1791A61917742" modified="2022-10-12" version="1">MGNQVYFAKTMEVSIFSQNVPLNQQIQHLGGQYYQHIERVELYKLKNEVTKFSTFLKKQFYLYNQAQNLYHPNLIYYYRFCIDPEITTNLIISRWYYQALYKRTKNICAYHFQYKSLIPEKDLWKFLYQISQGLQFLESKQQWHMQIQPDTIYIDQNMQVRLIPMGILRKMSVYSQVSNKQGKALLSPELIEQLKLNNINPVHNPSKSDIFSLGMTMLELKTLKSSFDCYSFNSNPPQLHGKLIEERMKESIMNGYSDDLIKITKKYASSRQSLQGWNKRYFKLNKNLNNQSNNFSPLKPTLNLTFKITIITAKIEQSNLTESQIQTPQSHLDLNQLDNISNFQQSYSFREKSDEELLFREQQINRQLEQQLKQQQQILQKKYLQQKKQQIIEDQQRKFFSLQQQIKQIAVKLEESHQKPTEIPNSLNQLDNYNYQINLLPTPPITFASPIKRTLNDLTENIEIQNRVNYSYIINLIKNNQQLLKFIVFQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9Z1</accession>
    <name>A0A8S1K9Z1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9Z1"/>
    <dbReference type="OMA" id="FRVIIQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="55" mass="6443" checksum="550B719AC6EEB2EF" modified="2022-10-12" version="1">MNNEDEMEKQARLMAEKYKDGPKKKGALVKKTQERTQFDSATHEMQKQAQQHPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAA8</accession>
    <name>A0A8S1KAA8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VASt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0180264</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAA8"/>
    <dbReference type="OMA" id="NGFHDKE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF4">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="4">
        <location>
            <begin position="145"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8051651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="410" mass="47453" checksum="B390FBC17076179C" modified="2022-10-12" version="1">MSAKDLLIQLFGLPKGEIIFQDYSCALKGLISKYGRIFIAENHICFYANLAGSKTNLVIKLDDINKLDSKNKNDIDIILKDGRVFCFNGFHDKEQVYNLMNALISGQPLQNQQTSQTTTDSAREDESQIENAEVEIQFLQQGASMDQEICKFTFSFSLDKFFEFFFADDALVYSIADHRQSEKDTDIQLTKWTPVEDNPQMFQREMKNVIKLTGVPFKDKSRMHKLFTYKKEADKLIYTCTTHTLDVPYGNCFQAEEKWEVSQLEDNKCLLKIFASVVFTKSTMMKGTIMSKTMSGLKEDYEKWINNVKIRLEAMAKSQKSQSSNINHEYEDSKKLDDNILNKIMQTSQSHQQKQNISQSQIYQGRKSELLYLLSIFLLIIIMLIQIGILNKQSQKLENLEQLVLQLQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KBD6</accession>
    <name>A0A8S1KBD6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0190203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KBD6"/>
    <dbReference type="OMA" id="KSPHANK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="299"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="342" mass="39634" checksum="C5208C94B915C430" modified="2022-10-12" version="1">MFLGRRIFRAFCSKAQYNIPIFTSSRGPQANKHIDDVLLDQMVKGFKKAYTDKFLLDFKKVVLPAKRVRSLDDHVIGLWKKHEVAVVIEGRDEFEDIDIVLDYYQVAAVKRRICANVAPIYIRVPGHDGDIRCTLGEIIKDPKSGWPFKVTLRRYIVGRPNLLYIPITILPNNSNPALVRGTDYDVHIEGVWINSYNATYPTKFFVDAKNIKTYRPYKLGDLQNTFPQGILLHERYKKLIHWNVVTLARDEDDKFFEEAEFQRSKKDDDLLIYRKPDDEGEETLEGEMLYQKKKVTVEKKQKKRKEKSLKKKVKQANQELQKEVAAAKEAKEAAAKEAAGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCF4</accession>
    <name>A0A8S1KCF4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0200346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCF4"/>
    <dbReference type="OMA" id="HIEWYSE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034338">
        <property type="term" value="F:short-chain carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000502">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF63">
        <property type="entry name" value="ALPHA_BETA HYDROLASE 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="110"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="393" mass="45948" checksum="FF1FAECED0E0A4BA" modified="2022-10-12" version="1">MIKKLLSIPKWKFFAGASFALLGQHIYDCLYNTEIEVFYQNTTFNKDLLSKCPNIIGHFNPSIWSMNNIFGLFYVTLIEHQMVPLQKREIVTLKDGGQVALDWKINDPKNVVLVLHGLTGGGDCNYIKDTLERLHNAGYTAVCFNNRGVGFTKLSTPQYHNHGDPSDMMEIIDLIKQRYPEATLQCVAISIGANLAAKYAGIMKEKCAFKSIVCIANPFDLLACFENLDRWYNYLYIYYLTKHFKWLLNKHLDVMNACFDEHNIDIKKVLEAKTPYEFDHYFTRQLQKFESVEQMYRANSSIQYIKDITVPTLFINTKNDPLIPYKFVPIQLHRHMPNLIFALTQKGGHIEWYSETNLVTVKRWIMDPTLDYLNYFRDLPMDKWQQYIQQPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJ84</accession>
    <name>A0A8S1KJ84_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0230232</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJ84"/>
    <dbReference type="OMA" id="LQDNKIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="97"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="149"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8055540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="387" mass="45058" checksum="338419A6F80C32D9" modified="2022-10-12" version="1">MFKVLIALKSQQQFKCTVTMGGKTKVITLPQTEGLTFNVDQLSTIQLKYNDEIFRIQINEDITETTGEYKFTNVSGQYYIVLIQDIEKPSKLNIKQNAQTSESPLKKNDTERSLTASTKKISFKTPTNKQKSESNQKMIQSFILGDNLMEQLKQENKELKQRIIELENQVITLQEQNEQRNDKVDQSKQEVSTNDIKNDFQNLLRLQQRQAQENKQLLKQLIDLDNNYQELLEKKSLVDQEVTKLKQTISQFDCQITQLKSRLTQFEIDGLSQNIQLNLQIAQPIENDEIIDNLIKLILKMRATGSQYIPIKEDKLDSKLAQIIAKDHTQQYQFIRLKQGLYLIGEDQYQLFLQDNKIMITTEDGVSQLQDFIKSRKRVKSINIRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJ98</accession>
    <name>A0A8S1KJ98_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0220212</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJ98"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="382" mass="45635" checksum="E10ECC9B55250211" modified="2022-10-12" version="1">MNYLPKYLTDKQSNKFRKLLIMINYMCWRNESRKSKSILLVAGCDYQQKYFNLHIISNMQNKVNKEISIDIWFINIVIGLRMEKMGGEGLRTKVTQKTLQIQNDFHIQYVIFLSTSERISNKKSHESIAGVNYFSGLPGYQIYYEKSSIFLITASIYCYFGFQICKLTQFLKKIMNMNFNQSYANMVFFIFQQYACNFHKHLYNNKVEQQHLVKQEEGVEQEVKQEEGVQLSGQMEDESLNQLKQEKKYQIANENQNDYNKIHIFPGQSKNYYKNMGQKIVRFIFEHFQDDQNVMGDTYIKQFIKITSQGFNRNAILKLKKSRIARKILKLFFANYKWVKPFISQHKAELDLYFRYNTQVYSPKKKQQQSKTLKNDQIKQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KK81</accession>
    <name>A0A8S1KK81_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0200354</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KK81"/>
    <dbReference type="OMA" id="MNDHIIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025:SF3">
        <property type="entry name" value="HORMONE-SENSITIVE LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="340"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8053486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="584" mass="67899" checksum="30A6140DDF0701F3" modified="2022-10-12" version="1">MNDHIICNTLYFLQKCQLLLSQIEGKQEWINEINECQKELELHKVNHPLYEYCLLFITEKIIQPFLQDLDTLFNCQDVVKEIKKYELNFNYVIKRLSLIKEFIPNVVKVPLDDLFCKPQTDDEWIQLNRYIEYVNLDDPQSLSKQFDQFYDYIAMGAAYCTLGQKYSNTLISLIVQNVGGAAMLIKKQTARNIKTKEITNPSLSFVKGLWSIQNNSTFFKNFMKFTNCKIKSHLKIHVPFLFDDQQFKYHHDNNTYVLEKFNGFKMVDDLYNKLQVKELLQKSKQQKIKVRIISANKVILNNNIDYSQLVQKVIEISSEHHQISQIIQQNNKITKIKKVVLHIHGGGWVAMSSFSHQSYTRKWANYLGDDAIIFSIDYRLAPEYRYPYALEDVWQLYLWIINFSQFYLNIKVEQIVLAGDSAGGNMALGICFRAIKYGVRIPDGLLMAYPAVNLDLTKFNPYLLQGLIDQVAPATVLKLALYEYLKDTNGKPDVDPYLSPLIADDSFLLQLPKMTIMCGQLDSLTGDTIKFVNRLRKLNKQFRILIYNGINHGFLNFEVPIFAVPAIKPLIENSCDLLKQLFNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKC4</accession>
    <name>A0A8S1KKC4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein-tyrosine-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0220023</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKC4"/>
    <dbReference type="OMA" id="DPNDGFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948:SF2">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE DDB_G0269404-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="28"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="91"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8054425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="35047" checksum="9BB520CA01D29DF0" modified="2022-10-12" version="1">MSKWLDLSLTCTQINFKCQQPLSRSPMDSVFEIQGKKLYLGNINAANDSQYLKNHNVGAVLSIIDTSDIKLDRSIIHLWIAAEDREDVQITRYFEQAANFIKDHLQHTNVLVHCYAGISRSSSLIIAYLIKYAGLSLKEAIIKLKNQRPQVDPNDGFMDQLKQFEEKVKNQKLCKSENATKSVSKFLNISTSIEKNNNNCQQSNKGIQNFNIYSRLNSTTNIKAKNQNNQNITIQKTKTSFLDKTCSPTNLSTTNKTGKTFISNNLYEDPLKQYQKIKNSQNLFNQQILGMKNIYSQHGRFHSMNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKG1</accession>
    <name>A0A8S1KKG1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0200279</name>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0200280</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKG1"/>
    <dbReference type="OMA" id="IWRFIRE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8053340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8053342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="272" mass="31794" checksum="368275DCFA4E313B" modified="2022-10-12" version="1">MQILEKIGDGLSSVINKCIINHKQCALKSFKECSRKLREREIKILNSLKHDNIISILEFDQNYNWYTTTLMKIDLHQLIITHGSLQSITIQQILLQLSNALLYMHSIDYVHRDIKLENIMLDSEAKLFIIDFGLSVCLHTSKQYPRHCGTSTYMAPELNQDEKIINSNSLKKTDVFALGVVIFILAYGYPPFTIAIQSKCKFWNTIIQQKWQQFWNYFDKLIKTPTNPDFKDLIQNMLEPDPNKRFTIDQVNKHPFITNQSNLEELKVKLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KMY5</accession>
    <name>A0A8S1KMY5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0240352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KMY5"/>
    <dbReference type="OMA" id="AHNFKHS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053328">
        <property type="entry name" value="Uro-adherence_factor_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34592:SF4">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_31, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34592">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="2">
        <location>
            <begin position="25"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8056519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="632" mass="77462" checksum="65B1477B1F1BF9CA" modified="2022-10-12" version="1">MPKNHYNSTKNKYHKTTIELPYAQNVIPVKSGIHNLIEKAEKLLREEKFSSIHLVAYDKTAPNALIMAKILKEKFPKLHEITLIESIEKFYHYEPLEEGLVDVNISVKIPSIIIKLTFNPTQEEMAKPGYQKSIFDERSAQLLKYQDPMMRELYDNSSAADTQQEESQEDEDQEQIEEDQVDNRDGERYYRSQVDFRSSLQESMIKHKDIYATPQQDQMGQSNKIRTQDQNYRQEFDMQQNKNQKYDNRQQEYFHYAHNFKHSKVPDDDDGFEEISIPRQNQYSNSNSQKFQHQPPYQQQRQQYDPNQQYQQDFVNSGQYQNNYNKPVRNTEQRKKRLEEQLTDSQLQFFNQSNISNQQFDRTQGSFQNQDGFGQFNYPNSKQNFIREERNMINMEQQNTQNFHFNRDIRDDRDMRDNRDNRDNRDNRDNRDRRDNRDNRDNREKRDNRDNRDNRDNRDNRDNREKRDNRDKRDNRDNRDNRDNRDNRDNRDNRDNRDNRDNRDNRDNRDNRDNRDNRDNRDNRDNRDNRDNREKRDNRDNRDNRDNRDNRDNREKRDNREKRDNKEKNDQRDNKDNREKNYNQNNQEKRKNNDNKDQNYKNKGGIKQTGQNEIINYLDKRRELNEKMYTPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUR1</accession>
    <name>A0A8S1KUR1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0270291</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUR1"/>
    <dbReference type="OMA" id="WINLIIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="850" mass="98097" checksum="08011C41D3220210" modified="2022-10-12" version="1">MNKDINPIQITKHQYSPQIQKQSLQSLKIVVPICIIFSLCSIVIAFMWYRSNGLTEYLLLGLNNVAILIVCIFSFYWSLTFQIVRDLINQQEGYPLSTIPYFHSLFTQILCAIQTALALSLIEYNSYNQFILILPLTIIEIFLLLYLANVSRQFNIREYGVQRLIVYASSAINLIFLAFAAYYLKKVISQINYQMYQVSSTNIEISFIKIILLTFFLLSILVAIFNVKKIKAYFVTLSYIVIGLCIMAACVNGLLIRRIQSLNLELSLPNGCRFAMQTISEISLKDELQCSQKYLNLDLSPYLPCDQDQQSYEWESNTNNKLGCINLNCCKAVQNYLFSPINSLTIWINLIIMIGLVQSFNGAILSESTFKKYKMHIVGDGVVFIVLLIFAVLAVCTYNFTPDLEIRIQHALVKEQSVLNQITILPTPVYKDFDKQEKLIGFHNLQSCQQITAVMLQKINFQTDNNMKGIILAIQGTNGQFTILNEENSQDLSIIVDNELTKEFFQGQTQLHDRILIKGNVVEAEKFMKNNLYFCSDYPLNADFEILKEFYEIPKKRILEGVSKETQKFADQAFKFYDIKVTIEDANNFQSISNSQIQIYDGKFLTQSCQIIKQLERPLYELITDENGSVTLNNLSQGNTYTIMIYKPNYKRTCSVLDLQRRIPKTNYIFRLTNGIQKHSVRVILEWSSKSLNLDLYGIFKMNEHACLTGPLSKSCGGMEHISISKQDQHIEILDITQLEPYTYTLFVKRFLTRQESLDLLKKQEINQDWIDSDPHITVYLNELKYPLVEFRLPQITNQQMDKVDMTWMVFQIDGIQSDAPNSIQKMISEISNDEIKMNTSSKKSYWPNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWB7</accession>
    <name>A0A8S1KWB7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWB7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23313:SF0">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23313">
        <property type="entry name" value="TSEC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="248" mass="29518" checksum="6B7A3F212BB4F4F1" modified="2022-10-12" version="1">MDSDAIMQKMAVIFDDPEQKLLNEISEINKNRYKTQMQKLNELKEIQEESDIEIQKQKKLIDQLEKSLIIESNKSKQYDEILKQYESEGKSLSDESKKYIIQITQLSEKVENIKKQYLQAQEKQDTMEREQQQQLKDLDNWSKNTKNLQNELQKKDQKQIKLLEELTQVKIQQQEIKQVESNDDLQKQLDKFLSDNKRLLKQRQEVVAVFKKQAKIIEILKKQKLHLQTSTSLDLTEQDFAKIVDFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWT3</accession>
    <name>A0A8S1KWT3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0280305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWT3"/>
    <dbReference type="OMA" id="ESHHNIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="215"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="488"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="524"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="600" mass="70842" checksum="63777421604A1C82" modified="2022-10-12" version="1">MNDSSCSWDTCCDRGLLNQGDCMCDYGFFGDHCDQQISKIFTPQYYIFIGLYCLVFLYTLINASYQLYNKIFDKNLTIQEKSQISIKLLVASPINIILTLSCIFSLVKLIWLILDPLQLYKGKTIIIEHILHDIAYTLLFYIYGYLLITWYSMYAEICFYLSNQDQDDNYFNKESNNWICQHYKKVVKLRLFLVLLVQTAVSIFTGLRLEIKYPHFAVTCYSFLLINFIIFIFEFALYGIKLQRCIRYQLNQFYNEKKKKILNLMNMKEELQKQLEQKQADAQFQSSFQIKKVENSPSQNSSRRPSVDLPQVLVPNQKDRLRKKNVTFCKNNSNFMYYRQESHHNIKVNQNIKEVIAEQTENSERETNIIQNINESCCLEESIKQIDWIYDQQDKQIIENVIQKKNQVVRDAKEKISCDQVNIIVPQKQNNRKQVDISNLSDQSQDQTQYTQIGQQIDAFEQQKYLEEQQLSLKSSSLEADKKILQKIMLLVSLGITYEVIFGIMCIIALVSEAIYSTPIGTMVYYYVSSTLQFLSLFNVLKLFKDFRSQQIKNFIWIQKIGNKKSDLNQNYSFIIPQEYKQDDDMLRKFESRINLITLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXW2</accession>
    <name>A0A8S1KXW2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXW2"/>
    <dbReference type="OMA" id="MMLNHEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035813303" evidence="1">
        <location>
            <begin position="18"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="99" mass="11443" checksum="1BA5A5A4104945CC" modified="2022-10-12" version="1">MKMVILILILTILNTQAQTSLRGKSKIEQNEELSFLSNDYTSELQEYSLFVQLTKPFIETAPMDVMMLNHEMEIARMMGNPGITMMNYVLEESPIRPAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L243</accession>
    <name>A0A8S1L243_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CNNM transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0300028</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L243"/>
    <dbReference type="OMA" id="FMKRQHS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF97">
        <property type="entry name" value="METAL TRANSPORTER CNNM-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035765214" description="CNNM transmembrane domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="5">
        <location>
            <begin position="38"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8060225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="429" mass="48868" checksum="265DFB4080AFC446" modified="2022-10-12" version="1">MKYLHLFKLVVFLHSSCLVLGDEIENKDEPHHHRYSPSEGIFWLFLLYAFGLSILAAFCSGNTQGLLSIDMLNLELKLKSGTEYEKKVAQILLPVISQHHLLLSTLLVGNAFACETLPIILHQLAPDWLAILISAGIIVLFGEIIPSAFTTGPDQLVIGMKMIPYVKILQAILYIICYPLSLLLDYILGVHLHQRYKIKDIRGLLNLHAQDSGHGNNAQLARDQMQLMDSMMEMRKQTVMKNCKDIKKVFMLNADERYSSDLKNKIRIKGFSKIPVYQGSNKDQIIGTIQAKSILKLTAQDYGRSISSLLQMQEPLIIPKDTTMLEMLILFQHERKSLAFICDKGKKVQEYDTLNISNLIVEGQYNIIALIGLTDVFEEMLDFEIEDEDVHGTLCDYPKQKNKYTELKVITEEQEKQGRGRELKQKLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L259</accession>
    <name>A0A8S1L259_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0310144</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L259"/>
    <dbReference type="OMA" id="TQMPQLY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="4">
        <location>
            <begin position="57"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="121"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="284"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="364"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8061111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="574" mass="68240" checksum="BF55E46E2BE32A03" modified="2022-10-12" version="1">MANPHIPVEFNKDDDELKCHPSQSGSTATPTLFENTNNSKAFCAQFFEDQLHQDDDEFDELNQQLNQVLLSDSFKPSLTQAFTADMSSYLSHEKLTKNSRKMQLEYQNANLKEKEFVFNTYIKNDIENFSLDKYRHYILEKIIEVGPPSHKNIILDRIYKQIHKLIKDLYACKVMQKGLEVMTFNPQDSPEQLENYLSFIHQDNAQMKKIYIDKIANQIIQKSLEILEGSNLLKLLQILQKYILNNNKEKFELSTDQYGCLIVNKIIDIYPKQFELQTKQICNDIIVRAIENCSCLTRRQYANYIIQQILEKGQEVHKRLLMDQYLIKDFISMSLDKYGSNVAEKAIIYAGPQWRLKLWEEEVSISESSFKKLINDQYANYPIQRLFEYLEQQQRNEFIALLHKLHGNNQLNNHGQIVMKFAQANYSVKRYTQKIVQSELNKHNKNQDKCNNNYNKVQKQQLNQQISQLQMQQQQQLLYQQQCQQQMQQIILQQSQFKQFDQQQYQASVMQQMLMFQQQQQQLLTQMPQLYNQDMNYIPYPIMTQDQQMMMLRWIQSQQQLCNSNIKFQNGQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3I1</accession>
    <name>A0A8S1L3I1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0250312</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L3I1"/>
    <dbReference type="OMA" id="FICERNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="279" mass="32772" checksum="ABD40D9E01FC71EF" modified="2022-10-12" version="1">MQKTQLMNFLQNGSNFYPFGDKPNNSYSPEGKFKPFICERNQVGKLVNNESYNFSSIRKNTKQRTSEQINQIDTDSIMRHKFRTELESPKYDPNTKYVTLIPTSLNNEDLQKPIDIKSYTLNHNQVERGQLMKKQQKGLNHKHQLISRSTKTLTSQIPAIGIYDPLPLIKHLPNIQIKSKSIQKLPEVWERNNSIVTPKQGLKHCESEMMFAKQIAQINREVEQEMKRNPNQKFAVQQFDINENMNLEEAEQMRNRMKQYFQSVKDSLTKIKNLINVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L574</accession>
    <name>A0A8S1L574_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0320234</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L574"/>
    <dbReference type="OMA" id="GREMPEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="457"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="481"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8061911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="534" mass="61957" checksum="81F7DD512B80F48E" modified="2022-10-12" version="1">MPKYIDVLTIEQALIKAGNGHSFQRRALIIMGLQYMIAGMLHIAMTELFFHQQQYKCLSEDLNYQNCTEGAFCEQYKTKENKVSLVDHSDLGSLIKYFLMVCGDKIQIFILVTSYYIGGSMGSLYYGEQMEIRQGRYTVMIETLLMMGFVCCISMLSPSAYILSVALFAINFFQRGFFNSSMILFFEISSENLQKFGPALLLTCYGIGEVISPRLIEMFNLNWQYSMLLLFGLPAVLFSVLIKFMQESPRILVIRRKFEEARLTINYIASVNERQMPENWYLEDEIRIQELKLKMKDILLEEEIHEQPKGYNFSSIFRYKSIRIRMFCLLYLYSIVVLGQFQTAKEIERFSRLERHHTYLLLSLVSTAGYLISGYASLNYLRKEVIKAILILGAIFHFLIAALPLLILNKTASDLITFYDKLVYTFIMMVLVMCRLTLSFAYGFINVYALEIFPTSLRHYGFCGLTFLTEFLFIFQDSYVIFCHAFGLNSSIGMFALLLLGLLTLQKLRETQDLPLKENVDEMEDELINNIYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5F9</accession>
    <name>A0A8S1L5F9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0290298</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5F9"/>
    <dbReference type="OMA" id="KVCKLEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="329" mass="37909" checksum="15E70BECA88BF155" modified="2022-10-12" version="1">MNHQNNRSYYQLNTSNILSTKRVPSLTPPKNYHNSNSKDKITKQIKDKAANSILKAEGLNFPIQNALFLQQQNTILSARKATINLSESSDKLRKTSPNQKSPNNPRLSTMQKNKQISISQSQQAIAQVLAKMQENDNISYINKQLYQIPEIESTQKSTINSREYTNSSKHKTEDYQKLLKENLILQKKVCKLEQKILELQQLNLQLLNLSKSCDCHLSKIRELEQGINNINQIQDIVNPLQMQNVDHFNIQSKGEQQKKRSSYCQEYSDIKPQNLFDYQNSNLIIEQLIEQYHINAKELAVFKGLTKVMEQKSLPIPSVLKTLSLVINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6L5</accession>
    <name>A0A8S1L6L5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0330103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6L5"/>
    <dbReference type="OMA" id="KKCHEFT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8062371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="580" mass="68809" checksum="6A138C3B00BF3A39" modified="2022-10-12" version="1">MFNNNPQKYITRNDIKEIKKKFDWIVIHSANSKIPGVTLEQAMQIVAQQIDSSPHIFSRWVSRQGRRNVATWPQILELIETYNQQQETAFDNQIYEQSSFQFIEDKFMIMKPSYFQITNQQEKQIFVYYIENMSYVKLKQEFAILIFNNKTLALINVHNFKPILVVSAQSIEKTKKKQADEFMGILQRLDQQQSKDSWKKQINFYTKSIDNSFHIERLDTNSSIKERLDPILQCKRDIEHLQKVVNLNPWDPQLRPLNPDPKMQLIRKRGQSEHSKRYQVFEQENYKLNSSLQVQLICSKDNNIILVLENLVLQYYQLEYDYKNGDYSLKKCHEFTLNDKIDQIAITQNKWIKQEHCLILKSKQMLVYSMPDFKLLDKIYVGQQADTMLSNKIIIICQYDGLFSIIKENLRKNQQEQYYSVGLQKQVKFNDLKQSVSSLDYSSKYQFVIFGSMCTLVSIYDIRKGQFIHQFSVLNTSQQFNQMNDKILRVFVFDSQNQYIIIFTSGYVTIWDIQRHKKIQECMKQLPQNKTQQQYFQGLFLEQRGMLLLGGFNLQSWISKINPIQIQDTILDSNSSPIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9D5</accession>
    <name>A0A8S1L9D5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0350161</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9D5"/>
    <dbReference type="OMA" id="KESTQCY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044244">
        <property type="entry name" value="TTC27/Emw1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="559"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8063829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="819" mass="96873" checksum="DCF66A42208E0919" modified="2022-10-12" version="1">MEIKCEYNLQQLLDKGHLGVGLKVILFFADNINDQALDIIKQCCLKILETQHKQIVFDIFQILYGLFIKENFTGPSYHYQNADRKLFIENILIKQVIPKCQINQISKEYLEELGLIELLPLINQISRVSEYEIYHSQANQEFILNKLQCCGELFKRVIQLKHLFLVCKQILELLHNTNPIYQLWWARLNFMHNQFLDNPVQELKDQIFNSFQEFLDNYPIGPKEQAKLYTEFSYIYNYYYKYQEAEACLFKAQQLLKVRFELTGKLGKKTKFQEEKLPQLVAQIIDQESVEKEIQELPKVQEVLQQYASQEVNLEEESILFDKPVLDGEIKQDSLSLEEQIVLLGLIHHYKRTLPNDEILQQQVQAYLNLMLDKSNSWIVYSQGLLLRSLNEFNHLKRMERALIQMQTLVDQFNETQPDSDLKALNLFYSNYPDYYNLSGILGEQWMKVGMMSSGYEIFARLEMWEECVECIVGTGDTQRALKEIEQITAKGFGTVKMKCITGEIKQDVKILKEAWKDSKKRFARAQRSLGEYYFFKEKNYEKSIKSYRKAVKINSYHQKSWYIMGCAYMRLNKLEEAIKSLGEAVRINENDGEIWGNISSCLVALKKYSEAQSALEQGVKYASTDWRLWSNLMGVSLRNKKFVRFYSCIEKLVQLDHRELIDEQIIQKITQTFAYQTDQLNQENIIQSNINKKRILKLYEYLAKEIGQKYFIWEGLARYTELLIIYDQRMAELENKQILDLLPYYNDIVQYRLKSCQMLQILNWERDMTICQTLVKQLTSLNQDVQKIQDEETIKNVTLYLSQQKSRLETILEKKINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9W7</accession>
    <name>A0A8S1L9W7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0360055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9W7"/>
    <dbReference type="OMA" id="GSHKQLR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="266"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="485"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="546" mass="65965" checksum="656539383EE597EC" modified="2022-10-12" version="1">MIDYIKETGDILITRSMSSFQQNSDSSPKNLLGSHKQLRFPTISNSLLKSAIEANYLEADQRNNIIRNPNHLTVFKMHFDTNSPRTQTAMEILKLNVQDLQVKDYEEFYQKHKDPLQNLISYLQYVTGKYKILNDILKKRNQIKHIQSQVSKQINSKNIKYKDESISLEQTTELKKQSYEDFHSTILSATQKKELFDKKLIKASENYNKFLNLVHDQINKQDEYYSKTAPEMYQKAERLKQKQLELVRKKLRMDHMKVDQICQKVKKEEQTQYREHQKMVQELEKDHNLHQERKQKQLQQAQEELLEQLKKKEEKERERQLKRQMQMNLEQSMIDQVMDSIKKKSDYMNEYLHKKQEDEKKKHNQTIKIFEEKQKKLQESYVQKENQNVQNYFSKSTQRQIQINKHEISQLKQQKSLSLKNSRIMKRVEQFQESLDQKRFEELNSKLQETDEKLEEGIKRHQSLLDLRKIKLSQKNEHRFKLAKQKQMENMLEKIHKQNQILQKNLEINQKNDKIKQELEFLEKYKKEQLQDYVIQRSILAQKLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCW9</accession>
    <name>A0A8S1LCW9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Golgi apparatus membrane protein TVP23 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0380176</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TVP23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCW9"/>
    <dbReference type="OMA" id="EEWIYEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008564">
        <property type="entry name" value="TVP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019:SF7">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05832">
        <property type="entry name" value="DUF846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8065918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="25581" checksum="0D080C742C69660A" modified="2022-10-12" version="1">MQIADDQQDVQHSIPDLDHVNIKLGDDEKQVNQISSNTVSQVQQPQQIAIPIQQLPSKTQDIQFLSQLFHCLFKGLAIFVFFIPEGLLNLTYCFIIVVILSAIDFWTVKNITGRKLVGLRWWSEVKEDGSEEWIYECQVANFVPNPFNSNIFWFAQFGVVLTWGILILLDLIGFRWFNAVLAMTAFCLTGINFVGFYKCRGEHQKKAKEYMTKIGLKAIQQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LF65</accession>
    <name>A0A8S1LF65_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0360238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LF65"/>
    <dbReference type="OMA" id="YMYAICD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="19408" checksum="E99272678F841FA4" modified="2022-10-12" version="1">MILHTIIVRGSLILAEFSDSEEDYSNIIKKRILDVKNVQENQKILKDDVYMYAICDKDLKFICLSKEDDETIFVKLQTLIQAVSQIETDGNYSAKLTQILHQHLRKEKIVVIQDEKVDQQSPQQTRLINPPKLEKKDQIDFQKKLCFSVMLLILSVYLILNNIITNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LN29</accession>
    <name>A0A8S1LN29_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0390279</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the 5'-3' exonuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="QVVIFHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041385">
        <property type="entry name" value="SH3_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF7">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18129">
        <property type="entry name" value="SH3_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="8">
        <location>
            <begin position="270"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="8">
        <location>
            <begin position="392"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' exoribonuclease 1 SH3-like" evidence="9">
        <location>
            <begin position="1062"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1255"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="607"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1255"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1266"/>
            <end position="1285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8066753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1313" mass="155278" checksum="51D49894455844AF" modified="2022-10-12" version="1">MGVPNFYSWLMKRYPLCLYEHTRAQRVDYFYLDMNQIIYKCATDPTVLFKDQLRERDFDDVWVSIINYLDMIINLVNPQQLLFLAFDGVAPRAKMNQQRQRRFQSQKRYKYLSAHLQQIGLFQKNETYKNNQFSPGTEFMTQLNQQIKFYIERKYYEDPKFKNLKIIFSGCDVPGEGEHKLLSFIRNLQCDPNSVHMIYGADADLIMLGLLTQLKNVLIIREDLKFKQVASAAANRIVKIPEFLIINISIVREYLDLEFAVLKDKMKIAYDINCIIDDFILLCFFVGNDFLPRIYCFDIRIGTIEPLVDLFKKHLTNANDYIVHRGDINYKELLSLLELLQGFEKLCINDRQQEMLNYLNTKHENKQMIAKEKELIDNILKYFSLQEQTEGRKFYYKAKCQITQGDELDLEKLGDMCINYMEGIYFVLQYYFKGVPSWEWYYKYYYAPLCGDIVGVVQNIVENLQDKEEPIVLTKGQPYPPFKQLLSILTPENALLLPEQYGKLLTDKENSILRTPIDYYPEDFETDSYGTMYEHQHITKIPFLDCKIVEEAYNQIPDTHDQRNQQAQSIFYQYDSNQQQIYESTLPKHFSNFKSFCKKQFIDIDDKEQCQIAYEQQQQQQQQQQQQQQQIIEQFENFYQIPTLKVVNIKSSKLINIQQHEDDMKKFENQFLLLELQSAKFDFDSFIQDVIKNKNFVYCGFPIQQQCELLAILRPDHLNLLEGIKLTSISLLKDYSKKDHSKIYKEIRNKTLNQYEKYMKLDCSIFLIVKQYKHYIRDNNNQLSYPQEQYRELVYPYEFVFPDQKVKFIIPNFEDFQIDRQVVIFHEKQNGANGIIKQIDKKLTIQITAYPTLLGYDYIHSDIYYSLQIVSEKLQCSVKTLLNLLGSVVVNMEDKESKIADQLDIGLNIINRTNNQLVPELVRLPQADQYATGSSKYQKFKDLELSEKCVTILCKYRNQCSRVQQYLNQLNDSQRNCPINAKDLFPNSSDPNIDLLKIYIWILQLPESQYLLQGSSSKVAEIKIHKKPIQNFNITKKVDPGFVIQQTTQTFLPPFFIKHPTIHKIGDRVVNINYPFGIYGTVVGLLEQKEIMVQVLWDQKYIGFTNLGGRYDLLTCSTCKFTEIFNLSNEDWRLNLAKKGCHQGEFWDLWTKVYKPDFKSRAIEFNDQLKEQNPFKQLVELPVEIRPKVLIKDKDKDAPQGLELLQKLAQEQPNLITNQPLQGIKEEQIDQNEDEAQKEIKKLFLMQQQIDQQSQVEQQKQTQKQSNEEDQKVLPQQIEEKEEKQYPQQQIKVQTQQVKKVLTKKKDQQQLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LN45</accession>
    <name>A0A8S1LN45_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0410163</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LN45"/>
    <dbReference type="OMA" id="QATHLMR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="180"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="499"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="503"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="533"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="566"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8067782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="651" mass="75432" checksum="B1CA22AE1BAFAEA6" modified="2022-10-12" version="1">MKGEATQPKTQTQGSAIKNDKPLKQVKQSSPLKHQIEPEVVWPFFAPQQALVNQMRFWDRKNCKNVYPSKEDLRFQAIMLRVGKKKKSTKGYFFKLSKDGTLSYYKKESDKVAKGYLQITMDYVAHLLEQKSKKDSLLIVQIEKYQGLSISIFDGKTQLTLEFFNHLKDYCIVSGMDKLYTQMELLGKGSFASVYKVKNLSNEQMFAAKTYFKETFDQSQHKSKFVLMIKNEIMVLRKVIHPGIIRLYDVIQEKEKLILIMELVSDGDLYSLIKEKKRIPEKEAAVMLKGVCEALHEMHINEFVHRDLKLENIMMKSRDQYIIKLVDFGFAEPINHKELVSKAGTPGYIPPEIFKLFPYTDKGDVFSVGVIFYSLISGISCFKGKNYQAVLEDNRKCEISFMKSVWNDISSECKHLLKKMLARKPADRYTCADVLKNEWILVHTSNEYEQQADSELERRNSYQTMKKSHYSHYNSVDGSQPDFNNDFSYHTNDLKSFYHRNSQKTVKTQRSIQSIKSNNDKMKQSMRNKSAHSKQQQFTNFKSQTLKHKNKSAIHSNTGEGGGDQKSVRWVDKKNKSDEPSFGDKKENDSVKTSISLKIFEDFEAINQTNVAFLKMSPILFNLQKKPDSDAKINEAIKKKKQQATHLMRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LN56</accession>
    <name>A0A8S1LN56_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0420067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LN56"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8068259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="18732" checksum="894B9D1356093F22" modified="2022-10-12" version="1">MSRAIRRYVNSKEEMEYDRGLSAEEMQAAKLRKAFVQKFIADFDTNFYKTQEERDWGYVVRREYRYDVTYTSLVDGWACAAAVSMVRMFQTKRFSWAPYFVVWPIAYLYFQPIKFLKHNKKYFDMCNLGETYYLGRERNKVLVECNRILDREDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LP15</accession>
    <name>A0A8S1LP15_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0410320</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8068092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LP15"/>
    <dbReference type="OMA" id="TLMDFGF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF98">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8068092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="636" mass="75837" checksum="30EE004A63B73AD8" modified="2022-10-12" version="1">MSQYDLIIERIAITSGWLYEMEEKTNEKKIQLLALSPFEPFHQNFLQDEINFKATIQKTKLLRHYLRCVDLNKKYYLFEFVQGQSIQQIINKYRVKSESIPIEKINNYLKYLLEALYQLHKMKILGRVFSIENIIELQDNSITLMDFGFASELKQNQFNILAPPEIVSSMVETNGQRLNYSIEIDSWLLGAFLFNLVKLYPINSYLKGNKLNAYTYSNLEEYNLFLQQQIKENKYIKVEPSKYPKKLNKLIESLLTYDPKERLSFLDIYQSEYIKDLELEICDEQIQFYKNLNLTEIHQKIMLRDGITSNEFIQTSVLKQPLNPQSEIKNLQDIQSYEADHDFKIPQKIYLESVVPDVYQSEQVTQQSSHLLKLPYFMENQLEKFQRLFQILNLEQFRFVILNNTADEVVSLLSNKKFGLEYVIEYFLKKMAYLIMIEILGKLNSQKSQFTTSQEEWDNFQKLEKKTDLIKNINSQIQENINRLKNLNEQCYKDLAQNQLFNENIRNSVKQDKHFLQGNKAPETNIFGCTSNEFLKSGYRNITQYLYNYLVSKCQFDEMRTKQFSLILKTVLCHLINRIFDYDYINTQFKNIKSEKNNDITPETIYKFIESKENDEQKIQEIQYLVNKYFQNQTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LR22</accession>
    <name>A0A8S1LR22_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0420140</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LR22"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="123"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8068401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="483" mass="56660" checksum="8C2A2F9F95323D18" modified="2022-10-12" version="1">MRQKSQTVHEGVERKLQAVDYDKCNYKCLLTKKKFLGRGLRYLYLFKNSICIGKSPFVQIPERQFRINSELRIKWTYTKNKLKSVIFQVSSQHYEYYGTNEQLRELKQKCALNVFQVQIQDEYQAESVLGKGSYATVLELTNLCTNKQYAAKCIDQQRINEKKNGQKQLQQEIETMRILSEQKHDNILQLHELYIGNQNYYLIMEMAKGGSLLALMKKRQILFSRKDIKLIMKQLLEGLAFIHNHNIMHRDLKPENILFMNKDLDSLVIADFGLAQSVGSHPYTYPKCGTPGFVAPEILEQDSDLARYTVSCDIFSAGVILYVLLVGEPLFEKKDRKEQLELNRKCSINLSRFPSDQLDDLEKDFLMKMLSKNPDFRWPADNLLKHKFLFLKILQKVKQIFKKQIIFQLMPTFSKNPFIKFRKNINTVRDATQTLFQRQRNCTLIMQTNLRFDKSSQEQLNTEQVNGMGSFNLYKDEINQTNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSC7</accession>
    <name>A0A8S1LSC7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0420152</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSC7"/>
    <dbReference type="OMA" id="YTHFKDD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016287">
        <property type="term" value="F:glycerone-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07992">
        <property type="entry name" value="LPLAT_AAK14816-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052744">
        <property type="entry name" value="GPAT/DAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605:SF0">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="409"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="457"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="3">
        <location>
            <begin position="69"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="534"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8068423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="604" mass="69712" checksum="95723FE92BC91C60" modified="2022-10-12" version="1">MIKYLTTQLQKSDSSRKQSFNNYLTGWLTNLQSYSQSIPFLQYQRVVTHTFFRDIQVIGQENIPKDGPILLYGNHNNQFIDPLLIISNMTRKINFIAAAKSMRKPIIGNIGRALGGIPVERPQDLAKVGSGIIKKYDGTYLFGENSQFTKQCQIHDSILIQGIDTIILIVEILSDTKIKVQCEDSSQVRIKDNKYKVQPKVDQSQMFEQVWLALKQGKCIGIFPEGGSHDNTTLLPLKPGICIMALGASQKYQTKVKLQPVGLNYFKGHKFRSKVIVEFGVPYEINQQLIDTYTINKRQAISKLLLEIEQQLKSVTIQAPSYSHLSAVMIGRSLYLPDRTRLSPQDLLQLTKRFLQAFYKLKDHPEVNQVLTEIKLYNYKLKALGVRDWEVMRMQKRIYLDLQIIGFNIFMTLLCLFFAFPGYIMTIPITIMLNMYAEKERKTALAGSKVKISGKDVVASFKVLASIIIVPISVIIYTILFYLWLTAYNIVDEEYTFRYTIIFLLMWPIYITAMIRSNDGLIRHARKVNSQILFYLYENKYRKLKIQREELQNKIRKLVDTFGEEVVQDFNQNRIVQKNQLQSPKSVAELDIQNVFESLLELGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LVQ1</accession>
    <name>A0A8S1LVQ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0450046</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LVQ1"/>
    <dbReference type="OMA" id="NGARCIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="3">
        <location>
            <begin position="82"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="103"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="139"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="175"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="212"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="248"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="357"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8070075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="435" mass="50268" checksum="E29BCBE495554068" modified="2022-10-12" version="1">MNQDPDEKDMIGDLLDDSFENVGKIKKTYSSNRPPALQINQNYNQSPIYYPKVYSPQVQQKQNSYDLDLRLTKVPVQQSAPNSASKLKSFDNTVQFNTVDETSLDGDLISKCKDQNGARCIQKAFQESSQAIKEQIFNKLEKGLLSLSKDVFGNYVIQNLLEFGTPLQQQKILIFLQPHSSQLAFHPYGCRVLQKLLQNAHNTPDFPNLFDTFKTRVRELVIDQHGNHVVQKLIQLMDSDISLWVLDGIEGQVNKLVINSFGCRIIQKAVSISSNHPERQMKILIEIMNLAYELCISQYGNYIIQQLLKDGPFIIKNQIQQIIMEKLEEYSLNKFGSNVVDCAIKCSNNQFKLKIMELLLNQNNQQVLFVRLSTNAYGNYVVQNFLKFADQEIQKELYYKITNNQQLLQEIQQYKFGQFVYQMLTQKLELETFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LW19</accession>
    <name>A0A8S1LW19_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0450091</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LW19"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051630">
        <property type="entry name" value="Corepressor-Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017:SF28">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_98, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="4">
        <location>
            <begin position="200"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="544"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8070165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="573" mass="67501" checksum="945120B16B15EF20" modified="2022-10-12" version="1">MQKYLLDNLPLSLQERNLLYANGQQVYDQVMKDGGFVKVFCQRELPAYLKEDDWVSFYQSKVIVYEYKSMVDGSCIGKPYYYNYSEMLKIYEEKQLSWLKHQISILTIDPKALEMINVFFNFEALKQNHGNQTIDIRVQQPGYDSFLPCKINQQLEGMKIMDYVDYLNDPSKFIKKEGLSVDQRLFAVNVNMQGWDKEINHLKQFLPKCLRPQGENDGLSYLRQEIDGVNIPQIYIKTDGVWTGGHQENLSVNAININHGPADCIWITVDNENVQKLRQKVLELYDTDIFKNEGLWFKEPEFFLKNGIPFRYTLQKKGDVVILGAGCLHWVKSLGQTINTAWNQVALDDMTLEAMSERHKLNKEINYRTVIPYKNLMLDLYIHEKSLSQMTIERIQLELQNFFDQENRKLHLIDPPSSQLIECCKEVQLCSKCEHEIFLYFYHDDEEYLCLDCVQNKSNHKSLPISMKYRYHCFYFLLQAQPTNCNQEFCCPYTGKTKCSIQQELKPAQFKYKAQPKLNSMLHGTDKMKKKKFDRMDYEIDAKTTVQNDKQKTRGRKQSIDKSQPTIKRNRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LX83</accession>
    <name>A0A8S1LX83_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Papain family cysteine protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0450089</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LX83"/>
    <dbReference type="OMA" id="MANKCIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035795550" description="Papain family cysteine protease" evidence="2">
        <location>
            <begin position="23"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="4">
        <location>
            <begin position="29"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="3">
        <location>
            <begin position="122"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8070161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="38772" checksum="5AE347D3759673B1" modified="2022-10-12" version="1">MKNLYIVALLSISLLSLQLYNSEQQTDEYSKWKQHHQKLYQGVEDTYRKQIFHQNLQIVNEHNARYNQGLENFEIEANQFADLTFDEFSSLYLYSSYPDQEYINNSFQKTTQKQNKSIKADIPDHYDWSTIIQDYSQPYNQGKCLGSWAFAVAGSIESALYLGTYEQISPQYLINCVDQTNPCLEGSVKNTWDWVTDNQNYLDLEVNVAYTGTKGACIPSLDNIQLLNGYEMLNKDQFVQDSDYYQALSEHIVVSPLSVSSIYFQLYKTGVFDKPCGNNPNYDALVVGYDARTDQGSLPFYKLKMSWSPNYGEQGYVRIAMANKCIYEKQSYANKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5U2</accession>
    <name>A0A8S1M5U2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0480151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5U2"/>
    <dbReference type="OMA" id="HNFRYGF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="93"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="198" mass="23727" checksum="88A6B218D184E0B0" modified="2022-10-12" version="1">MSSEINIRQLLYYKQVNPNPTQQSLRTVKTMQHRLNQLRSTAQSDFRLTTLQTQTPIHNFRYGFNVDEHQNQNSQIHFLEGFDHPFIHINSYITESKKNIAQLRDQSNRIRLEMSNIISKQESELNQLQINFRESLNQKYKLLKEDNVQFIEEQYKLSKEYQRLAKSKQIMEEKHQLLLRRVVQLENILQGVALELQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M7P8</accession>
    <name>A0A8S1M7P8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0550086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M7P8"/>
    <dbReference type="OMA" id="AIYSIQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="506" mass="59504" checksum="C7EFC5001A09ACCA" modified="2022-10-12" version="1">MQQKSINLVHRRFKFPEIEGSNKSNRSKGSPTSSRNNTSQERDLSIDGGNSIQVFKIRNLQISERSETPKEDKIFSMSKSTRLRSRQEPNEDYTEHNQQELILPKIYSPIITPRKNVVSKMKDSIFKPLLSRQETIDHLINIKLQTKIHDQLKKGVNIKCLYLSQRTDVQINKIIVLNFDSLLLGKRKSFWEQQNDITMCSQIIGSKKCDNSYCACVLSKELKSTLYNLSKQFYIVFLFECAIRGQVWQQYLIESGFSIDAIYSIQQIRLLGGGIKMKQIISNFGSSDIKQLLYFGTIDCGSQYMKIPKEQFYYQIPIVQAKISLQIYLLQLQKSKSLDCKLLFDICMLNCNKSENFNYKRHIDVVNYDLEPIIKQMIQSEEEDFIQNNIFKSLKQDEEQQFQQEDEEDQEEEIDQKNALLIWLYQAKKLLLDYFKSINNEDQTQNPISMIGDILRRNSSIQQNYIFEDYEKQAKRYQLYKELKNSTTSQTFFRKQIQIDCLIIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M7S3</accession>
    <name>A0A8S1M7S3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0550262</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8076157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="VECTLIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1151"/>
            <end position="1197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1965" mass="230006" checksum="7F815653EF67CA1E" modified="2022-10-12" version="1">MKLKEKFYDKPDATTVSLLQSIKMNPYFQKLISFSLNCLFSQMYLPNNKHRDNSIYIFKEGYSILIQCIDNHRQSDQVIQNVCGCLNRMIGLHKFRDEFIARNIIDSNLSHVISSITNRQLSQEAIKSTLDLYQNLTFTQLQTDSLAKSNILVGFVQILIVSKPLILEMKRIFDLEYKMLIQECIIENINIHELLTIYIQLMQEYIDNIFVIKESLRILLLLVNNQNALKIIKQKQSIPLKIMSEILIQHNDIDVNDKGAQILQSIINIEQVQQSIQSIKSVDQQAEFSMSLIACLIPAIHNQINLDELINILFELIKGYKKIIKRKPILRDALNCLSRLYDQDQDYAVKLKQFLPLMMELYENEDAEVVAASFDLSRKLGTNLTIEELNKIIEKVIAQLAQLKGVKDGLRLLLTYASKNDKTFKAISKGKEAFFKIIENVNNQTSQEYNSKLLLLIHQEIKEYLIKKLSVIVQFIQKHYLWKRSLLYYIQILNKYPEQVAQLKSNLMISILSCKKNKDYISIVSTLKQVKTDSEAEEDDPEEVAIEFQQNEELEQQFIILVRNLTKQDDVKVLQKEIIGNKYNDELSQVMLEIQLLKLSYYVQIEGTFYLQNDYFTELMNYLLKFLKSYKAEMIDIVQCIFLLLQTMSTLYWNDKTGTLYTQKKTSQLCFDFLFQSLENEELMDSSLDTILFMGQGCKNTNKKQHYLLDIQDNQYVIQLISQLQILMKKYVQKERIQLKILDVIKIYLLFELFKQNVRDLPKILCRKLPEDTTTKEMGLETIQILSDINYTDNALKYVTLFQLQHQYDIEVINQVIPYIDTVQKYEGDSFLTEEIKKQIKQMINYFEEMEDPEIGSEYMDKLSVYSTIPQALEYEFDCNILDALVNVLKYCELEKNNDRLLCSSINILTQMLINNYIEPIELEKCFQFLQQKRHSENPVNLLTFYLSKGEAIIQTDYLQFILDLLEEYQNNKFVVANLYDILYRSCLKSKDISKILGDKRFIQKLILQTQELVIEQSNEEQAKKNLAWLENLSQSEPVALRIQREKGRELCQLIIKQNSAHSMFAFKILANLAKFSQSIPLSYFLDYLKQNQFPLEILTSLDILFENEEIKQEFLKQNGQQLINQWQNDYPDQCQKLHQHFKPKQPVIPRQSLLKQRQSLLQQRQSLLQQKQLLMQLRQEKEEQEKQQKLEQSQDKENINESQQMIQQFQQPEQNQQLIKDCLELAVKNPQQIIDEGMLERLFQIEDSWEVFRRLTKHQDIAEQVLQPNSKLYQNTIDELKNQDSKYAEQVLETAGQISQVPETLSEFAQTEGPVSMVNLIQHQADNPKVVEAGLDALKKLCQDEMILSTIGDSNGIDLQKDLLTKYQNNIEIIKKCDEMISQLSVSESYQDKIAELGIIQQLIEQIKQNPNTLEIVQPAIEALAKVVYRNADNGTTVMESKILDEILQSQILDDLEESLLTLINNMCYKNEEIKKQMKIEYLIKILKKNIQNYKQKSFEQCVNTLFHLASYTQNIQKMIEQKLLEIINEFIKKHQKEVDSILLLLNLELKIAAQVNVPIDIQTVECTLICLQFDKLEQQALRLIRQILANCYQFIDQLNGFTQIANILKKAEQSSCVLTLDVMLKMAAINKESISKFEQLNVGLDFSEILRVNKNDKKLYDQILDYSLKLFNKTNELANQLMIFIQQNEVTELQIDKTLLIVEQVDDNETFIPTLIELIQKNNHKHTKTISIILSRESKVYPNQIFVNKGIDACVAEVLVTKEKEVLMNLATIILDVAKSLEEYRQNIAEQFLNQLEKLNEPVLNELIKFLQGPKKQISKLTDEIKNFLQKGEILKMYKEDGSRGYYRFFCDETLQQIRCRQESTKLVKQKWILKLSQIQEIKGNYDKKSLSSSAFENQNWLVKQIHKMKHKVIEADRCFTLFAQKNVKPKTLNALCKSESQKNKWVEYIKILIDEQPLLMQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBP3</accession>
    <name>A0A8S1MBP3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0540061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBP3"/>
    <dbReference type="OMA" id="IITKITH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="160" mass="19083" checksum="7D3864CE7F47DDD1" modified="2022-10-12" version="1">MYEFWNGQDFKNRYPNNFEVQGNQFIQQIITNTNLILDHFNQQQFLKALPPLSEIQVKSLYYEAKQLAESSDELQEYIQNYMDFNGNIIKLNPQFQNAEKQQCAQLLLKMIQMVQGTIGDWYEFQHQQIEIEKFQEKVDLLRHNFSLSENQNCCGKCQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MD91</accession>
    <name>A0A8S1MD91_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0600068</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MD91"/>
    <dbReference type="OMA" id="FITIKHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="6">
        <location>
            <begin position="6"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="7">
        <location>
            <begin position="20"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8078480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="33279" checksum="2A89E12F4EAA59CA" modified="2022-10-12" version="1">MNDLIVPIVTIILSFISIISCGFVIYIYGRFRELRNDQFTIVLQIILFDLIYDLILFSDSIGYLFLRNTNFQLSEKPVLCQAQSFFSVYSVLSSTFWTSIIIHSLYHSLRESETNYYMQSYYPGLGYGIPLIISIIPIIIDAYGQYYPMPQTNCFFDINNENMELYIMIFFDIPIWTMFLYNIIVIILVIRIINKNNIVNQSLYTLFMYPGILVICWIIPGFVNLIGVKSVIWKSIDFGLGSLIGFFDSYWYCYTALADRLFYENGEFVIRIKDNTEEQELPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDR1</accession>
    <name>A0A8S1MDR1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0560116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDR1"/>
    <dbReference type="OMA" id="IHEANCN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035740820" evidence="1">
        <location>
            <begin position="16"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="270" mass="31142" checksum="B20EE851C25D04FC" modified="2022-10-12" version="1">MLINLLLVFIYFVNSKNIKANRNFKVPQITADYLVKCKNVFTYYLEQKNKIVLEEEFQAEKTEIDHISAADIQGIAEDKPIIFESPILLQSGPIRSTTLYNQFGFVQTWTILHHDDFENRNSMLGWNIPHRAYCELKGIHYTDVEGSPQNDHFIHEANCNVNNPSLQKTYNTNIEHNHIRVSAELTIFGDWNGDSINVLIDNKVAWSKVFQGEITQAEHPCPALFALGIPVQFTMEHKTHEFTISFQANHKRHPCDGSFGVDDIIVYYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEN9</accession>
    <name>A0A8S1MEN9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0610117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEN9"/>
    <dbReference type="OMA" id="PDQICEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="90" mass="10466" checksum="35374F6C37B74829" modified="2022-10-12" version="1">MTNSRVHAIDSLKPQSLLHIESPDIICENQLVVCKTALNIKSILKKQEGQQKEKKQLLFKDDLNKIYVVENWKIYNTPYEEDDSCCCLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MH85</accession>
    <name>A0A8S1MH85_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0570110</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MH85"/>
    <dbReference type="OMA" id="FLATAKC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000113">
        <property type="entry name" value="Beta-adaptin-like protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015151">
        <property type="entry name" value="B-adaptin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09066">
        <property type="entry name" value="B2-adapt-app_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="7">
        <location>
            <begin position="37"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-adaptin appendage C-terminal subdomain" evidence="8">
        <location>
            <begin position="661"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="606"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="606"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8076895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="774" mass="89303" checksum="732A3C5E070904FE" modified="2022-10-12" version="1">MRSNQAKGIENGSATKPSQYFEGNRKGEVNELKILVKNTINEKDDKKKREVVKKVIAYMTLGIDVSKLFPEMCMASYTNDLVQKKMIYLYLTTYAEQNKDMAFMAISTFQKDCKHNDPKIRGFALRNLCSLRFSGAIEFLMPAIKEALSDIDAYVRKTAIMGCVKVFYMQPEQLNNIEDQLYKMISDNDPLVIINAIHALNEILAQEGGMALSKKMVDYLLGRLKEFNEWGQATILDELSKYSPKDDKEMFNIMNTLEERLKHSCSAIVLGVIKVFMNFTKNKPQIYEQVITRVKLPLVTLASISEGNFEIMYTILCHIKYIASKGYNSVFASDYKSFYCRVDEPTYIKLLKLEILSLIACDFNLGDMLNELGEYVTDVDQEISKKSIQALGAIAIRLPDLATAIVKQLSSFVTLQDYITNEVILAFKDILRKDPKHIKDCLEIIQSDNITDSNSKIALIYILGQFGSQIPLAPYILETYIGAQESVELKHILLTSCLKVFFVRAPEMHQVLGKLFYVIINNENEDIDLRDRAAYYYRAIQANINEVKQFWQYHIKVDKFLEETIINKEALLFEFNSLSVIYQKNVNKFIKPIEYFNNLRSKELQDLNQEQQQTQASTTQEQTSDVIENPQTQSYGQTNDLLEISDQPQQCISLNVDNFIENFSIDADHFENMWTNLNDGATFTRMLVRNDIANETSMEQLFKQYRLYNIAAGEEENVLKMYFYGSHQCQVFFEFEINKNLSTVILNTRSEIEDQAYLVSKYVEDFLKQVHLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MHS2</accession>
    <name>A0A8S1MHS2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0590105</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MHS2"/>
    <dbReference type="OMA" id="LASINWE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05579">
        <property type="entry name" value="STKc_MAST_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000604">
        <property type="entry name" value="AGC protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000633">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000340">
        <property type="entry name" value="Serine threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="327"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="166"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="179"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8077981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="710" mass="83036" checksum="E335887A063F592E" modified="2022-10-12" version="1">MEHKIICRICNLPIKIDFLKLHNETCKTRAEYRRKEIKLNLQVAKVCENAYKKKHQIHSRGQKYLNSLRLKWDQMSPEYTQEIYQEYKILQVLISYGERTLNSEVDAKYHLISQNEFIQAQQIIRNPQILNLIEQMNSLINQRLDLCYKTKFQSIPSISFRLSKFSKDSPKSNSETHNKMNSFSDYLNSSNFSEDTQSDRKSISRPDTPVKSNSIINNLRKRGKLLEWPKRQSTFSSEGDQISEKPKHRQEIQKKSYFAKSGSSDSEPDDIIDTIVVVNESTQREDDSNFESACFMELQRGYFSDTEIIRLDVKNKIKERNIGLKDFNFIRQIGQGAYGSVFLVKRIITGDLYAMKIINCCNKRFERLLEQLKQERNIFEILTGDYVVKAYYSFQHESSLCFAQEYMIGGDFSKILINEGAFDENIARHYFSEILIALEYLHNNNIIHRDLKPENILLDQQGHIKLADFGLSELGINKKMIKKCSQQSFSQSDLSPSPIYQMNKGRSFKKSNIIPENAERRIIGTPDYIAPEIISGQSFSHKSQDFWSLGIILYEFLVGIPPFNDESVEKIYQNILKGDIEWPEIGNDPEEQISYQAYDLLKKLLNPDFTQRLGYVSIDEIKNHPFLESINWEQLRNTPGPIIPKINQNFQTFENVTEKVQKFLKKGERKQIQIINKLQDELEYLERIDLLVAKNENEAQLIQQQLQLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MM85</accession>
    <name>A0A8S1MM85_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0660070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MM85"/>
    <dbReference type="OMA" id="FIFQQNG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8081588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="581" mass="67309" checksum="794D8D0391911D42" modified="2022-10-12" version="1">MQYGLQCRKHPGNQVTFVCTKVNCNYGNLLCQQCINGNLQHKNEHKNYLNQLNEFIFQQNGKTKTVLIPNINNTLQELDKRLKEYNAMIDQETQEIVSDIAQLFKIFYESSDRAKKQIQEIIRKDLIEQQARNINFKQQLQEIQSNEQKRNDNNNYLFNTIFSRDQDKELPISEQSLMQIINKNGDLNQLRCDILNCLNNVVSTLQPTSNRIRYKYTQNHQQQFNLLKNDFIQSLNCLFQKLTDLTQDFHTVEDMKRSLYQFGSLDGSQSLKQSQVVQRTLGSSQFISTGNQSQRFYRSNSQSQILSVKTLELFPHLSPTIQSILNKIGHLSPPQADSLLSNIETVPPCQFPDGIIYEGQIQNNQRFGWGRAIIGQDYYEGYWRGGLPFGFGRIIMASGDYYEGFSQNGQANGRGLFCSGGYSYEGDWEEDLKQGKGQEILQGKYEYKGDFKNNVKCGQGRLIDLVTGNEYQGEFQNNQLQGEATIEYKNGDKYYGQVITVDGQIKRNGQGIYLWVDSRKYEGQYENDLEHGDGVFMWPDQWIYKGQWQFGMQHGKGIQYNPQGKEREGIWNNGQFNKWIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNL9</accession>
    <name>A0A8S1MNL9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0600248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNL9"/>
    <dbReference type="OMA" id="HYVNIRY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8078783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="189" mass="21566" checksum="710E85A8E42672D6" modified="2022-10-12" version="1">MRFSKFIGRILLAILYVNAGFSQFFNQDSYIHYVNIRYPVFYGYASNLIGSYLENIEILKPNNFIQYTPNLILVLGVTQILFGFGVGLGIQQAGYCLAFLTVIITGYAHNPLIYSNQVDINREYLQIIFNIGIIGALFLIRKNKNNKNVTIKNTAESEKKEQKNAEQPKQQNNEKSNQDNAKKRKGKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNP5</accession>
    <name>A0A8S1MNP5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0670078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNP5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="146" mass="17777" checksum="2A434D19360546FA" modified="2022-10-12" version="1">MFPFTNQVGDIYILCSQQRISNMINIWNEKFSRFHQIFWKQQNRVYDSCLDGDRIKKQYKILFGYNQKRKYYAEVILNMINIRKDLHISIKVILINCNYYFLYGFRDFRCEFFGVNKMIQLQRSIIIKYKDCALGYCGKNEQIDIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPF6</accession>
    <name>A0A8S1MPF6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AMP deaminase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0600184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPF6"/>
    <dbReference type="OMA" id="KEFWIGE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003876">
        <property type="term" value="F:AMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000035">
        <property type="entry name" value="Probable amp deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006329">
        <property type="entry name" value="AMPD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359:SF0">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19326">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8078655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="694" mass="81401" checksum="1E7AEC6D85E4957A" modified="2022-10-12" version="1">MSEFQRRSSQRQGSIEPDAQGLSIQYVAEDQNLQTQYLDLKLARDETKNQKTVFGNQFGYAAKTTRRVSDTADDEIKEALNFDILQIKPGKPYKGAKTKESRESASKILEILKLRDYYSFDGHEQSEISSKFVQHNAKNEPYIIWNRRVDVLPILENTEVLQENGIIFTKSGENIDVIVPTMLQFISDLINLMKCVGNNSIASFCYDRLKFLEQKFQMHEIFNHQNEQLDQKNIIRRDFYNVFKVDTHIHHSAAMSAKHLLEFIQRKYEKNGDDHVDINKDGTKICLRDIFKNISVDPIDLSLNSLDVQADKGIYKRFDRFNNKYNPLGTPKLREIFLKTDNYIKGKYLADLTKELMDQLEQQQYIGCEWRISIYGKSMEEWHKLGKWLIKNKLYSSKVRWMVQIPRLYSVYKKSGMIHCFQDMIDNLFRPLFDITINPTIDPFLYQALFQITGFDTVDDESLYEYFAISDLKQSPKDWAGDRNPPYTYWIYYIYANLYTLNALRQQRGLNTFKFRPHCGEAGNIDHLATAYLVSDGINHGLELQKSPVLQYLFYLKQIGIAMSPVSNNKLFCRYQKSPFQKYFQIGLNVCLSTDDPLILHLTNEPLLEEYAIASQIFDLSAIDQAELARNSVRQSSFEKEIKEFWIGENYNDRIAQKNAEDRNNLPATRFMYRKVTLNEEYEHLEKLNFEELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQ53</accession>
    <name>A0A8S1MQ53_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0570185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8077044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQ53"/>
    <dbReference type="OMA" id="QCTHAIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="428" mass="51038" checksum="BB680DF671B0E779" modified="2022-10-12" version="1">MLFSKEPSNKRKFYYRNILTSNFDFFSKCLTLKENQKYTKQSISNSNLIINHRKPNQTLHSPRSPVINNLETPRHIRFHTNNSHKLFEQKQISEKISQKQNTERRAPLHKRKIQITETSLQIEDYIELLKNKTSSNIQYKYSDLFKDQSFQTTSLHFKSQNPRKSKEEQRNHLQLLLQCTHAIAKIKDGTNIQTIINQCTHQYPNLSLIIDQFIQDLDVAIQLYSNENQLSLNLLRITGLDYKIFHVLFKPKQEFPQLHYQFKIDENFFQNYKFYKPTKYDIYYRVYGAGELPLEFQEQLGEFNPSIIRHKLKDSDYQAAILIKDQIPSIDQDFVRKIIYYQKNLQLFLKQKSSEVLSSFKEQLNLDEYNNNNILRQSILSKLIDSENLIGNFPHITKSNRYLFEKMELEQQNKVYSQRIKQSKANAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MRB1</accession>
    <name>A0A8S1MRB1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0580096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MRB1"/>
    <dbReference type="OMA" id="NHNIFTR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="435" mass="51496" checksum="E53D40A60BCB7026" modified="2022-10-12" version="1">MLKINFKETRQQTSYLNQIPQKSKFLEFRQCHTWHSKSFELPSNSLLQSNQQRRQLPKLKTNLNIPNTTQIQQSATKSSRSEKFDITDAISTTSHIIIETPVKNCLIRRVSQFEFIGSKQKRLNNLTTSIQVFQHSKDAAILKKKKMNRLKMQYKFTQRSNHKKYYRHHKSRSQRLPTILKITHKRAKTNSDQQYEGPINKLEPDLERKINLLLQRKRNSHLILRRKTCIQEPKNSVFVSRDKLLKYHEIMDKKFDQFDSSCESSPATILKVQFKKKQFIKLQQTNSGNHQYLTPRLNYEQNHNIFTRKASHSVIAQYVNEKNKKIKNIFQSKLPNIIQPSLNELNQYFLAQKRSVDIIRTTQQLIQSKSSGYLPQQIQLNNKQTQFINKQLKIINQDSQICSSTRIKTLPSDTTVSRNKINLQQKLWPYFQQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSU7</accession>
    <name>A0A8S1MSU7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor beta propellor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0670236</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSU7"/>
    <dbReference type="OMA" id="DIGNCDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002671">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011387">
        <property type="entry name" value="TIF2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Translation initiation factor beta propellor-like" evidence="6">
        <location>
            <begin position="202"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="450"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8082402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="484" mass="56641" checksum="AC9D07E5BAC960BC" modified="2022-10-12" version="1">MEQNIPHQLVIRNKNGVYLCNMLAKTDEEMQLINQDGNVQLIVLENHIIMFNKQSIKLHDYKQQIKELEFTNLQNIHLTESKQHLIIIDKLDQQSFQLQILMFPSLEQKGINFNIKSFKREAWPLLKYSSDDQYYFMIQNQKLHCYADNKCVYQTDIGNCDYFSVQPNTPKPLVVCYSLMDFNIKESFLKIVDINGKIKYEKKLHQTSDLEVIWSPNGQSLLINKGFHEDNTNKSYFGQNQVLFYDFPKNALRELPTYEGPIHHVQWSPSSKEFILLSGFMPSGAVMYDNTCKPLFEFGKDHKNKIIWGNGRFVMICGFGNLDGSIHIWDAKTLKQISNSKYKSASLCHWSPCSRYYVCAKVTPRMNVDNQYSLFDYHGKEIINRKFLELYDVQFRPSNIKFEERPPTPIKQVQQEPPKKQFIAFADNPLAQQMRAMKAGEGARVLQVDETFGKQSNNNYPPGYEPPKEKKKRVRKPKQQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWB6</accession>
    <name>A0A8S1MWB6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0720210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWB6"/>
    <dbReference type="OMA" id="SDWPKDE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="344"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8084688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="42439" checksum="A3B54410D2CDD573" modified="2022-10-12" version="1">MLRQVCGSAITVEPIKKSKSSCSTPNKEFHIDQIIHKYIGMDRTADTFTSSITTMFHNTLPEEKQLLVSPYKKLTEKDCEKYFTTFQRLSDRKSDNQESLHQDEEFHHLKTSSDYDSSHNSLHEIAQNLEQVFDSPEGKNNGHTDKSPEFYNPETKLKSLPKELPIIEENEEIINTHQRLNGLITQIDQKIGLHQVNQQVIEKIKEKYLPKKEEPLEFKDFPEFNPSDWPKDDDLDNLKIDQPKQQSPKQIVVKEIRVQPIRNITFNSNEFNDFIQEQFQTEEQEKKDYNVKQKNKNDDHTEQKIKQDDQNVSLQRKSQIMIAKNSKFKLPKLSEQQIRMTTKAIVILLILTILSQLFTLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N029</accession>
    <name>A0A8S1N029_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0730081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8084972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N029"/>
    <dbReference type="OMA" id="AFIWITY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="47161" checksum="BDAC678A5A4F5EAA" modified="2022-10-12" version="1">MNYIINLIVSLIFIWITYLVYSIKQFKHDQISDWILIFARRLLFYSSILIIFPLLIPNGLEYQHNLFLKDHKYLRVIQLIDQLSISERETLTAYINQEFSMKYKIMLLVPIVLLLKEIYLLVQINQKESQGKEANNNLAISHEYPTHPIIFQPKNVSEYVIDKNEEILRIESYIQNNIEKMKSIDEVKSSYLNNQSDLLNKLQFTIDSKKPEKILYSQYSNKQLANARHLGKIEESELKSPNFKTIHSQALIENSFAFDNQSKLDTQIQRRIKLIISELIIIQIIFGYTVGVQEKPIISCLFIMLSQCIIIDLFDELMLFIRKTIMGTCLLIFYLIFVISMFLGMYYNQYLIQICYCIIIAYCIKPFMNFYGHQTIKQTLMFSGISLLLLIVIKLLLLIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1S2</accession>
    <name>A0A8S1N1S2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0740203</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1S2"/>
    <dbReference type="OMA" id="FDCEEID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000015">
        <property type="entry name" value="Oxysterol binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF148">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035934034" description="PH domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8085572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="874" mass="103341" checksum="FCD973A453934BEB" modified="2022-10-12" version="1">MIIEYQLIWLIIMNISNFFQECVNAVRDPRPIQYQPSMQISEGEILVKGKTLGRWESKKFIIDEEKRVFALRSSNGKTRLKPYFLQEYQVENKEKKKDKITFDLTSKTGGKCLTLGIENEQKANELINTLQDLCNKIEVKQNKNETIQQNIQDHIYNNQHQMKINEIDYEIPSFFDISEIRDSISHIRKGQHNLDNFKLVLDNKLLIYRNEHNHHQFKVFMEFDGDCQNLIIQNLTDYNKINQWNAFISVQESRMIKELHEDKSFLICEIRELSNLFIFKREFQYLQHHFKIDNIDFIVQKQIDQKSYTKNYQGSLKYGVWGILTSEKTTKVYYFSEQSMQGLSYPDEDGYLIQLYLKQIINLYQYSSKFQQDLSQIKQNELNLYVQQQYQQELKQNDIQENKEEFFDCEEIDAMNDKLFMAGNDSNKNNDINDEVVRDVYEQNVRRLTSLPQQIDSLDNIQDWIDKKMKLFLSGNYNSYSINVKHQLQPKEYDPLQRQLLTQQEGGHYIFKKDFIREEKNGGLKVINEEKLAAQKAVIKFLLTRIGASLMMGKSITSISMPVTIFEARSNTERVCNSMGFAPIYLEDAAQSQDIYYRIKQCAAFQFGFIFMYLSCEKPFNPILGETFQGFYDNCPIYCEQISHHPPICAIQMYGRKYRIDAQLELIANFNSNSVVGRNVGTLKITFENPHQVVLITLAPGSLNGTTYGDKVMNYLEKQFIIDIKNKIIAEFTFNPDKKYCQFFDIEYLNQLDYLCGAICDVTDAAIQKYHKEGYRKYKGLDLKQDIKQIRHKIKGIWVNEIKIDNQKLISIHNDFPVKMQLAQYPIPSDANFRMDLLFWKLRDFDQSQQWKEKLEILQRQDRKLREQKGKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N222</accession>
    <name>A0A8S1N222_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0650040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N222"/>
    <dbReference type="OMA" id="HYYHEEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="366" mass="43848" checksum="A030028D67F61912" modified="2022-10-12" version="1">MNQDFQILQGIYQFQSQFSKIIKGLQDEANKYNKCFNQAQKDIQDFYNKLLQSSQRMNKQQINNDQEQQQQCEKSLTTLKNKYGQLSKQLNIFNENFLQYLMQKSEFDNYYQQIQLSQIILERNTPLIPLQNLSWLMNSPLQLDVAKQLTSQLSSIIQQQNIQFNKNEQLSNFFCEQTINIQKQIEYLEQQKILKAQIELQEQQKLAEIEENQRQQQEIQKQKEELEQKNDIDNEQIDNFENLSETEELTQQCQECGQIIKFNNFLTQCLHYYHEECIIQKIKANYQKRSIYCSCNTLINSRMIKDVLEHSKNDQNQLSFEIFQYYQIKQLLKKGNKTFTECKCGFLYITEKEEQIDNCIVCDKCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N2X8</accession>
    <name>A0A8S1N2X8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0710138</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N2X8"/>
    <dbReference type="OMA" id="THRYPIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="3">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8084023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="482" mass="57118" checksum="5C7C0C7777C19EF7" modified="2022-10-12" version="1">MFQEQSFGGQDKSTIECRNLRRHGKCTVSGCPYSHSAASVKPKVLRKVFQGQSGIISDPNQQQQNYRNNFQNRNNYNNNQNYQGRRDFQFNRLNQINNNNTQDRLNYSNNQYQQSQSTQQYDNRQQNQPSYQGLQQQPTIGLQLLYKMNVENLKGFLRIININNNFYFGLINNRGITFYPYNETKGIDILQKIDFVIQFEVEDAFIQEYTQQNNRKELYCLAVVIIESGLKSVLIYPDFLNSQTNFVKISDISNTYLKYIHVNTLNSELYTFSSDGFIRVFDLQNTQNSLKYYAQQTEVFQSIFFLNETSKGSNFLISTKNGNIYHYMNQQFTKLHSLTEQTLIDITIENDSKRVYLATKSQEMFNIYIINENNAFEGPIYTHRYPIQKLIGFKDFDQINCLLVSDNRGQLDILKEQPSNDQVKFLRQIQYKQIMGDIKKIMFFKMININKVIEKMIFVQRQKDPQRQESTNFEFYRFYQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4R8</accession>
    <name>A0A8S1N4R8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0730165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8085121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4R8"/>
    <dbReference type="OMA" id="FTIIQSC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8085121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="78" mass="9219" checksum="9C6910F4F147B187" modified="2022-10-12" version="1">MNNIKNYFSTPLSLMSKNHLNSEIDDENTENNQYISAQSFTIIQSCLSKTIIEDYPINKLKRSQENQQSNWKDSFKWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4U0</accession>
    <name>A0A8S1N4U0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0770231</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8087052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4U0"/>
    <dbReference type="OMA" id="QWIEISY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="15322" checksum="E2B77E0CE58AABCC" modified="2022-10-12" version="1">MCISGGGLYDETGNELKLGNWVETTNNFKLNSQVIFSGEYKNGKKVGRWDFKYKKDNKPFFKIGGGSYDDSGDEIKLGNWVEIMGNFRDYSQVTYRGEYKNGKKVGIWKEMKRDNLSIKEEFIIVQEIKYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6T2</accession>
    <name>A0A8S1N6T2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0790079</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6T2"/>
    <dbReference type="OMA" id="SFIFYPQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF115">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8087722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="141" mass="16511" checksum="DA95F83F790B14D1" modified="2022-10-12" version="1">MSHNEQSEMHPGMQMMDMRMYMIINWDYKVRFVFNDWKSTTHGEFVGGLFVTMFFCVFLCLLPVLKGFADKQKSLFLKIVWQLLAATQSSITMFLLMTVNGWVFLAMALGFGIGSFIFYPQMPISEKHQREDDQQVLERVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7D5</accession>
    <name>A0A8S1N7D5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0810093</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="QCENEAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="415"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="501"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="561"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="638"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="745"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1020"/>
            <end position="1040"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8088490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1131" mass="134291" checksum="4681E5AE73F6B74D" modified="2022-10-12" version="1">MRSYVLSKENKYLLLQDWWQKPTKRNQYTHSRIPFIISGSHISIILNIWSLFIQILTLWITPFMISFQQENSIFQVLFIIILFQVFFDVFIKINSPLIVNGETIYDKTERIKDFFKKNLLEDLIYIFTLIISFFPIFDNIQLQGGFCIIIIFISYKKLNQNYESLYEILYLKGQYHYAIDVISLTILIFSYAHVMACIWHYVGEITIDQGQSWLIQRNLQNSEIWEKYNISFYWATMTMSTVGYGDITPTNQFETLAANLMMILSSCMFGYSISQIGMILKSQYEVQQKYKRSIIIMNAYMKNSQVDLQIQSRIRNYLKYQCENEANENKDDINKIITDLPIGLKQELNLNLLQEMQYIIEMIQMIKVFXNQLVQKFKNILLMRSYVLSKENKYLLLQDWWQKPTKRNQYTHSRIPFIISGSHISIILNIWSLFIQILTLWITPFMISFQQENSIFQVLFIIILFQVFFDVFIKINSPLIVNGETIYDKTERIKDFFKKNLLEDLIYIFTLIISFFPIFDNIQLQGGFCIIIIFISYKKLNQNYESLYEILYLKGQYHYAIDVISLTILIFSYAHVMACIWHYVGEITIDQGQSWLIQRNLQNSEIWEKYNISFYWATMTMSTVGYGDITPTNQFETLAANLMMILSSCMFGYSISQIGMILKSQYEVQQKYKRSIIIMNAYMKNSQVDLQIQSRIRNYLKYQCENEANENKDDINKIITDLPIGLKQELVQDVQMNIIKRIKILNNQFSQSTLKQLSQYLIEFKFTPGDVIYHRNDLNDQSLYYIQEGIVNIYEENSQKLLQSLKAGDTFGEYQFFTGFQSQTSTKSQGFTKIFKINRQSLLQLLNQNSKDFQRFHHIKDNIIFNKNFQIVQKCCNFCKIADHINIDCPLLSYKPDILQRIKKAELKPSIQQRYFLQRNNQKVRCLMLHSAIQDTVEEYKANLSTTQIDVDMKLFSTYATKDLSSNCQKESLLDRQQTKPLPVAPLKKNNSFLIRQRNQSQQKKVGIFVNLKSVFRDSDRQLKSESEDDQQNNSPKNQVSQQFQIDFNDLQMNQFDDQFQTTNFNIDRLMNYFGYMPMSNVIYALRKYERLYKYSKVKTINYPESESRKFSIPLYYSRRDSINELLKEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDD3</accession>
    <name>A0A8S1NDD3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribulokinase/uridine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0780027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDD3"/>
    <dbReference type="OMA" id="EVWFVEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="188" mass="21833" checksum="C70470F1346534DC" modified="2022-10-12" version="1">MILNQKEDKQIIIAIAGVPGSGKTYFCKNVICQQYPDVKVIPMDGYHIYRKDLNEEGMKRRGAAFTFDHQRFKKDLTNLRETGTGSFPDFQHSVKDPVENAIQITKDDKIIVVEGLYLFLKEWDLKHLFDQKFFIDKEFNAQMIGQRHYVCGIEDSLEKGIQRAIENDKVNAEYILQNSDFSDVTFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFQ0</accession>
    <name>A0A8S1NFQ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0850107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFQ0"/>
    <dbReference type="OMA" id="EQLMQDG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="44"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="100" mass="11618" checksum="14F282E9D7A8686D" modified="2022-10-12" version="1">MQDGGNVQDRDLFMKEMDNLISQNQFGQAEVQLTKYIEKNEADSDAFKKRSNLYLKNGKYIQALHDIEKAMLLDSSMPCCKSSYEHYQIKKGLMRMSNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJ77</accession>
    <name>A0A8S1NJ77_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T0770055</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJ77"/>
    <dbReference type="OMA" id="KFDSECV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="124"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="448"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="450"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8086704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="494" mass="57243" checksum="EB29E96FF328B911" modified="2022-10-12" version="1">MKSKFSTDSIFLQTDSEFWRNEQIQIKEQEFKEDYFLFYSNTKKSYKNKVLKRHGPIIKKLKKNGLELWCDVTNAIIEMLDIQHVGQGLRITKNSYSLEFFGYVDGWFKELKKFCIQKQIKNQFIISNKLGCGNYADVHRLIRKNDEKEFAVKIYDKLSNKFDSECVIKELDILRKMDHPNVITVLETFESQKYIFIITELLISGNLDDILSKTALSEDDAIKGIFKIIDALTYIHSKGVIHRDIKPENILLRKPNLEEIVISDFGLAEYHNEEGQYKYHRCGTAGNMAPEILKDLSYGYKADCYSVGIIFFQMLTQGQSPFIVEDYQETLQRNEQGQIDFSVVQSSPSVMNLLKQMLESDPFKRCSVQEARCNTIFKKFNRQTIIIKRKRIYQDHTVSSYSPRSTSIYQSPTNQASPQIPISPSYKYVGVKNLHSIPLRLRSHERKLFSSPNSTNSGANSAKLQIKQKNSNFKINQKNNKTSIYQGMSSNKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJ94</accession>
    <name>A0A8S1NJ94_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0820213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8089065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJ94"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="73" mass="8758" checksum="8EAEDABBE8C42635" modified="2022-10-12" version="1">MNKEKREKFKIQKGSQKKEEESKFNEVNKIQLIQLIQRQELLIIQLNQLVKREETVIIRKHQVGQLIIHLLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NLM2</accession>
    <name>A0A8S1NLM2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PACRG-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0920085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NLM2"/>
    <dbReference type="OMA" id="YCLIATH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019399">
        <property type="entry name" value="Parkin_co-regulated_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207:SF1">
        <property type="entry name" value="PACRG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN PARK2 COREGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10274">
        <property type="entry name" value="ParcG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27309" checksum="D0A588745A288AA8" modified="2022-10-12" version="1">MQQQKKSQPPAVLNRTRSLGKQQQQKPQQQQQQQQQQQQPKQGFTQLPSQKLTSIKTVDPFSDKSKQKTNSFAYVYNAGGIPCRVNHGCNTMKLQWNQGVEISHLPYDPLMVTCFEGLIEEAHPYCLIATHAIKDMLNEQNAQEKVIPILTKLVWPLRTALSSQNDKVFMSSLEILKLLSNTVGSHLNTHLKNLLVPLSKRMDKKNQKEIIQDVLRQIQDNGGPETLKLIKVAIPTYTNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYG7</accession>
    <name>A0A8S1NYG7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Biogenesis of lysosome-related organelles complex 1 subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0990007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLOC1S1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYG7"/>
    <dbReference type="OMA" id="WQILYNN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009395">
        <property type="entry name" value="BLOC1S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13073:SF0">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13073">
        <property type="entry name" value="BLOC-1 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06320">
        <property type="entry name" value="GCN5L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="111" mass="13024" checksum="6D2A9D44A1AD5A61" modified="2022-10-12" version="1">MFETIMKEYNQNKKGLDKQDEILRRQIGMLAEPMTKKVLHGLNANIAILHQNEQIIEKETKQLLKETAQLQKESQQWQILYNNLNETLKKVGDIVNFAQIIENELQELCES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P267</accession>
    <name>A0A8S1P267_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1030150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P267"/>
    <dbReference type="OMA" id="KFRHKSK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="88"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="325"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="479"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="163"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="325"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="479"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="495"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8097275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="59634" checksum="D5D15592BA9FDD1C" modified="2022-10-12" version="1">MSHSSMEQRNGQLLVMTIQIDDKRSGRIDVFEGDDPEILARDFCIKYNLNQRVAPLLIDNIQKQIQIAQKERINMVQYLSQKKQNQRQTEQFNEIQNQQQGSTPIIAQSQQSPMKQESMSQTVYDRLYQDAHNKKLKKQLQLSENKSSSQIMKSQESEVNYGLILYQKGIQKKEEKLQKAESARKDIQKSQLQECTHKPQINSLSSKIAQRSPKPVGEHLNELAKIIQEKRENAQNFKMEMEQQSCSFHPQINRVSSSIVDEKKKQSQIAIPHYESLYQDMDVKQQKLNELDRNYFAQKHTFHPKIDQISEQLVQGLSFEERRQKFRNKSKERNSSADEGNMFRPRTGRPPEQRPENLFDNLYKDAQLLAQKRAAVLSFSREKIMNQSRVRASQKSEFLTQQAIINSLANIFDQIDTDNDGEIDAIRINLGNLNQTVIQILQPLISEMEEGRHVLTKSEFIESAMRLVYTLSNKEKHDLLKKPNSKKSDIPIYTFNPSINSRPQKNSRSKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P3P1</accession>
    <name>A0A8S1P3P1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine Rich Repeat family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1040142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P3P1"/>
    <dbReference type="OMA" id="CWICDEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="926" mass="108600" checksum="5F27AD6B5C90BA11" modified="2022-10-12" version="1">MHQITTLETDTPKKLNKFSMRSLALPRITLVGFEKSPYHSGIHEHSSNVPKRYNRQYYKIQQSAKERDLISDKDVYATLSNNQEEPYLDRKPKDLVGDDAYKSYYHTFKNIKRILEQNQFENITNSVQTNLIQRAEKLKILPCKMGLIKLKGDKNSIAIQNQKFGDKYVEVLSEGLRTLPTIQDFNFNRNRIKEHGASQLFPLISKQARIIEFQTNTIGQKGLDPILQCLPLQTCKIQILNLEDNQLGDTLISDLCKAMSKNPSVEMLNISKNNITNASYISIKQMIEQNDTLLELYLRWNSIKGSGGLEIFKVLQTNKNIKVLDFSYNLLGAGNVIITALKDFIIENKTVQHLDLSANGFTYQDCLQISEALKSNHSIYGFHFRGNFGYVDSKGFLIIENNMKNYNSIHIDQRIKGVSPNPKPYEHSTHFEKLKDICWICDEWQMSTFEWIPNESGACSEEPIFIHFDYEGFEPIFLGKPDSNGNFKTHRMIPTGDIEYFYTANSIQIASQTAPIKQHIEKFRTKVTIADQVINVLIDETNLEKFTKSKPVIEDWYPTYDVLPRTQDPIYIPAKRKKQKRIWSFPISIWAPKYKFDTEELLRKCFERDWACCKITKFVKKQEEQDQVKEMLWQAYKPMRETYRFYASVNPTGDVFSMSVNPTSDFVNQCQLIDGKQLKLADVDLKFIATCSASSIDWKGNYRNPERSLVRYQMMEFLVRLSDDKYVRFNPQINIVQATKMILDQCMPHLSQFDCHKWRAERYFVEQCDDVCKKYKWVIDYVYMRNSQKKVKPGQPPFMCLDELKDICNKANLYDENFVERDVNLAFNLSMLTQVDELESDRLFQMQWIEFLEAIARIAEKYSPIAIGKKEEKEWTYEQRFQQPLYYKLEAFMVYLINTLVDDETKKNWKQPTISMFDEVEEDEYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P3V9</accession>
    <name>A0A8S1P3V9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1040187</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P3V9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF12">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="8">
        <location>
            <begin position="28"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8097698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="231" mass="26688" checksum="C953B7E8115935B1" modified="2022-10-12" version="1">MIGQISLFNKTINPKIQQNTYQLIIFQNNLYQIIKKRPQYYLLLQKVEPEQVVNILRNLFIGFDNECLTSNIYKLCTIRDAFGIVDIMQRNAAQEAKNVFELGFGMVEIIRNARQIIGFDGLDMRRGIYTGKVIAGILETKIVRYDVYSADVMISKKMKSNGEKGRVQVSQETKTSRSPISSCVQFSFQQIDQFESINRQINGYFVEPQKNDSGFDDSNQSSEREQFKTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P4N1</accession>
    <name>A0A8S1P4N1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1050059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P4N1"/>
    <dbReference type="OMA" id="ELQFICP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="281" mass="33351" checksum="808EF8147F695505" modified="2022-10-12" version="1">MSIIVYGAKIMNVYCGFQLTWKTCEAFKKQNVQEGDALLIKWFLLYTFIQFEPVLMLFLELIPFGYFAPFFIKFYIMLPHSRWHSLIYGVFEKLEMDEYTNRFFIYFTKIVKYFLVRFLNLIETCIDLVRVEELTELEKQVDRIMEKILNKKQSIEVTKSKIRFRNSETKGFKNNYVGISETKSQKLSVQASVKDMGRFVDYILSFNTQNSELQFICPQKNICVHLKTLISIDINSDKCLKVVYSENDGNLELMKLKKSEDIDLWLRPVIENLIEKISQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P4P3</accession>
    <name>A0A8S1P4P3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1060008</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P4P3"/>
    <dbReference type="OMA" id="QCATKSI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="778"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="811"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="372"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8098017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="909" mass="107189" checksum="34DAEEDA59BF3B8B" modified="2022-10-12" version="1">MKRKYNKLFKTEVESDAFGDLVTFQDFESKLRTFIDNTSKFTKGKQKVQMKESNSQTDLDFQSILLDKIIRKSMTKKDLKTKYIDLCSEFGIDYYQLLKLESLELKQDYAIQCATKSISKSLENLLTKYKQEKHEPSVPNNSKQLDLYGWMKVKSQYKKLKKILQYALVELVQQNSNQDKIKMFFAYINHFIQKRHKTTESLNFSTAHTSRIRTQQNFEEKLYLDPPQFQFSKPDLFFEWNHAIEQGYLSNDQNIHKVGYELMKKQNSKGVNYQKREKLLDQCQEGNYLTAEELKYLASTSFLINKCVDKQFIQRAINFLDSQVIHQIEMNIAEQVLQTFYYLKKNLTKYRKEWLEKVNIEKRKNIKINSELNQKKNQLLSNSNQIRNIKKKIINQLRDVAKKKQLQHEAEKKKEKYLRIQRRMGNPLANIYVQKFKEIVLQVISKIREKKVSDKERQKEFLERNEAKPPPKAIYTIGNEIYDVKKSKRAFHPPSKVMTTLSEDKYKIEKPEIQKQQIKRYKLGIRNYTVTQDQFKNFYAGGPQLPRKFQSSELQHLIKNDESDTNFPRKNNWITSEAEVQAANKIKFAIKRYIQKKALNKLIVKSEVKKNRDIEIKIRKCMQAKKFFEELHKELDKRQSERLKDISSHQPPKLPIDKIEKIPSSGALSIGIKSSGQVSQTQRTSKRLQSHRLSLYKYIPPQEKVDENSLDTARRAEMEIKEIARKKKMMKFSKQKEMSEAKLKNRKLIVAAKMKNLQVAMTSGFQYNQRDTQLCDRFGNTPLYYCAKNGDEQFCRFLLNLGANVNQQCENLNTPLHMAFQSESKQIIIDFINKGGNLSMVNADKCTPLAFGSEELLKSLNLCNLVATTKQQYLESDNNNVFSKKEAKWMDQQFEDGLIYDRYKYKTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7L0</accession>
    <name>A0A8S1P7L0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1080131</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8099026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7L0"/>
    <dbReference type="OMA" id="ELDNDYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="294"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="340"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="319"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8099026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="423" mass="51574" checksum="9EE0D6C28763C9FE" modified="2022-10-12" version="1">MKKATDGVYYENEYIANQEDKLLAKMRKKQERELEQLKLYEQQQTEQERRKYSILESTRSKQQQVEQALKEKKQMIELKRKEKEKEKLLQVQKIEQEKLKELTEREISRDLILQELQSNKNNQQKIIPDKVKPSIIKKIAEGIINTQQEEILMKKIQLDKLQKEQEEQIKDLRERKEQLLKELRQAKRKQLKKVKQRNEEEKNAYYQEKLQKIEQYQKKLDDIEITQQAKIELIEQKKKEKMEKLKRVMENYKKIMAEKNEKAQLKVSQLEEKINQISEKMKFTEEKIEKIEKERTRHRQNIQSRNGSIQNNKSQTFQEKIRQKEEEHLKSITETQSISKIKLQQEKQKKEELIRDHKKKAASILKEMVEIKNKKLKEKLDRAQQFKDEKEKLFMQQQKLAKELERQKQKLLLHSPRYKAEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7M1</accession>
    <name>A0A8S1P7M1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1080039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7M1"/>
    <dbReference type="OMA" id="YFQQEDE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8098844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="152" mass="17990" checksum="3257B1A6B72CD16D" modified="2022-10-12" version="1">MHEKGQPSLPSLHIATNPNMNSTKRIHARMIIEPNVKDLISLPRAQQLGSYNQQFRRVPLKLNPKVVQAFSPYQSYSIISFSDFLYEKHKKEKQLQKLKEKKQLEKLKDIQHKEKIKYFQQEDENNEDIIDTIMEKVRIELGQQIQSNFITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCP6</accession>
    <name>A0A8S1PCP6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1140054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8100990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCP6"/>
    <dbReference type="OMA" id="KHPSEQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8100990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="225" mass="26280" checksum="BEF507E51DB366B1" modified="2022-10-12" version="1">MIPVQILKNIQNQNTQHRQTNSAFLKSRKHSPITHLIVTRHPRVSQQMKEQSNSPSTQIKTSSSQYPQIIQSVEQMKRFPTEANVKSIKGSTIQISNALKLFSSKHPSDQYQNTLKFSSFLYEKVNEQSIYTKTTTGESQENDKEFNQDSFIVYNNVLHGKNLEIQKLEKELKQLKQENKISKFRKRLLNYIVGKLKFKTKIIELINRNNLIKSLVDMQFVKKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PE31</accession>
    <name>A0A8S1PE31_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hflx-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1160039</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PE31"/>
    <dbReference type="OMA" id="VCHPYRE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01878">
        <property type="entry name" value="HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001888">
        <property type="entry name" value="GTP-binding protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030394">
        <property type="entry name" value="G_HFLX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032305">
        <property type="entry name" value="GTP-bd_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016496">
        <property type="entry name" value="GTPase_HflX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025121">
        <property type="entry name" value="GTPase_HflX_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229">
        <property type="entry name" value="GTP-BINDING PROTEIN HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229:SF8">
        <property type="entry name" value="GTPASE HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13167">
        <property type="entry name" value="GTP-bdg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51705">
        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hflx-type G" evidence="6">
        <location>
            <begin position="353"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8101602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="607" mass="70402" checksum="208E32DFEA1A4020" modified="2022-10-12" version="1">MLIKRLSNIIVKYRFSKEILDFAKSTPPKQQLNSQPVNCMVLHPVLYPNKGPELELYMAEEAIGLSKSLSWTLEQGPFWKDEYTQDIRRSQGKIDEEDKDNQSQEQNQQKDFQINEDDLGHEWRNKIIRNSIAKSSLVKVPRIHSTTFFTKGKLAMLGQHIQSKRINAVFINHELTPLQTRNLEKLWTQYSKGEITSVIKEDNNENIDNNEAIITDEIESDNDFEGLSVKVFDRFTMILQIFAKRSTQGVSRLQIELSFLKFAKTKLVRSGSAFVSLSSIFKGDLMMAKEVYVEVVSAKQRRALGKMSGSGESELQLQRRLIDDRIAKVKKLIDEESQQRSKIKRKKLIHTVPRIALIGYTNAGKSQLLNCILQKEVVQSRDLLFQTLDTTSKSIRLASGQKAIMLDTIGFITDLPHDLVESFKSTLEEVEDADIVLHIRDISHPCTEQQKQVVLDVLKELGFNQDFYNKKMIEVWNKIDLMNYPIDYEFIESQDYPIVPISALMNINIKRLLQVMEEKSNQIMNKKLYRIKYNIDQHFERLKWLYENGNISGVMNEIQIPPIKKGDPTIIEYDVIIDEITYNRYIATFQPEMRIKKNKGMPPSNWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGX8</accession>
    <name>A0A8S1PGX8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1180048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGX8"/>
    <dbReference type="OMA" id="CDNFEVN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039918">
        <property type="entry name" value="PPP4R4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21467">
        <property type="entry name" value="PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4 PPP4R4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21467:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="660"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="408"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="660"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="702"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8102335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="718" mass="85145" checksum="7C74B25B17AD35EE" modified="2022-10-12" version="1">MMDYSCDNFEVNESRITLNKDEEIKKLTLEEDLPEIKRTQIILMKGQQRQKLAIYNNLHRLLCLGFDQLYQYIRGDIMEQSEEVQIIAARSLQNCKIKPKEIIQLALHFIQLYQWKLAQAWMPVFQNLVLLIDYKEFQSYLEKMILQFSEPKQPEIGRWVGAKMIVCISPVLKDEMKGPILDRARLLCSDPDHEIRELVADELLTCLICNLSGELVEQYFIDKINELLYDTQIKVKKNMVRTFLRFQHRFPRNSYNIKGTTVFIDCLSTNNIELLSVALQYCGETFVQIQDLINDEFKNKFTILYQKFGQHQNDEIRKWYLYNLPGILLHLNEGPYASQILMPYADILIQDKSHQNKLLACKILHEISKQFDYSYVLQKMWHLFECVIQSDDLDCILVINLPEMYKLLQGNEENQEKLKTQFKDINQQLQKVYQKCLSNYQYNQLFLEHLLKFLHLINKKHFEQYILPVIYKEQQNIHSDLLSMKCLAKFYSVCQDYEIQMKIKDTMNLMFFRGNNNKRVRYIFWVSNMIPYISKKRFQELNLNQIISYSSDKAASVQIQLIKALPQIYQYLEPIEYNLIKNVKGLTQQSLSKEAFLQIQQRQQQNLNFQDEQNQLIQQEEAVFTQFTQQQKQEKGNDLIQSNEYANKYLKKPIPKLVQQQSKKTPNASTSVRLANQSFGELTPQQQQQQQFKRVRRYTDATKSTSKPSSRPSGFTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHB8</accession>
    <name>A0A8S1PHB8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1180098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHB8"/>
    <dbReference type="OMA" id="VEYLIYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="203" mass="24427" checksum="2B561334F837C8A4" modified="2022-10-12" version="1">MNELQIFENYRCLYHQIQFLNCLIKQNDQEQKLNIHNHILNRVKQILNLKDQNYMRMQEILTLNVINCLEDILKLESSEIRLLYSNIKILVEYLIYLNEFISQQAQQVLLGDVIIDIQLMNKQIDECIKKCNLIITKHKHFFEEYNELQEIISKTTNNINESSQLLMIIIDEIKEQHLINEVQRRCQLKNEDTIKNLELQLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHM4</accession>
    <name>A0A8S1PHM4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1180163</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHM4"/>
    <dbReference type="OMA" id="INSLPEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03044">
        <property type="entry name" value="GST_N_EF1Bgamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000006">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1010:FF:000001">
        <property type="entry name" value="Elongation factor 1-gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000148">
        <property type="entry name" value="Elongation factor 1B gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001662">
        <property type="entry name" value="EF1B_G_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00647">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01183">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50040">
        <property type="entry name" value="EF1G_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="83"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="EF-1-gamma C-terminal" evidence="5">
        <location>
            <begin position="263"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="215"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="215"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8102544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="427" mass="49579" checksum="34F6341FB9F34BFA" modified="2022-10-12" version="1">MSIKLHSPSGQGRTHKILVAAKLANLQLEFVDTQLSEKNFQVFQNKFPFGKVPVLETQEGNLFESNAILRHIARHSQGLYGKTLYQQGLVDQWLDVTTNELETDVVAAAIQVLGHIPVLPEQYKTSLGQINHTLTIVNTQLGKSKYITGDSLTVADIALAQVVTFAFTLLFGEPQRNKYQNVLKWLNEINSLPEWKAEFGRPRFPKNTFILSEVTEEKKEKKEKKQEQPKQKEQKEQPKQKEQPKQKEQPKQEEDDEAPKKKEANPLDLLPPSTFNIDDYKRIFFAEKDIQKNIETLFATIDLNGWSLWLVRYNKSENEGKQLILTNNLMKGFINQRLDQNFRKYAFAIHGVYGDEPNLQLRGAWLWRGTEVPKEWKDHIAYDYHEFIKVDVNNAAQKQLFVDYWVKQEEDVSEVEGLKARSLMYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PI91</accession>
    <name>A0A8S1PI91_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1180062</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PI91"/>
    <dbReference type="OMA" id="QIPTVIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF155">
        <property type="entry name" value="DEUBIQUITINASE MYSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="98"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8102363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="178" mass="21119" checksum="A393CDA855EB6826" modified="2022-10-12" version="1">MNPDLMCNLYSQGQYYNPFYYLQQQQMLQYMMFQQLTPTISMPSYKYETQFILNNGTTHQQIESPNITNTAQNTHSTEVDYELEDKCGLVDDESQSRVNGHWTKQEHLLYLEFVKSHESILKSKYDKKSKKIFKLMSQFIPTRTATQCRSHHQKFNPLAKGKKKNRQINKQIPTIIHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PII2</accession>
    <name>A0A8S1PII2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1190032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PII2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="217" mass="25560" checksum="E2A54969E0AAD7EF" modified="2022-10-12" version="1">MTKSSDANEQQRKTRKYQKINKEERRIVLKLLLNDKLSLQEVANRLKLKYCTVRTIQKAFEKDGRIGKKETRKKKLKVQTILKISIVNPFTFQLSEPIVSSEVSQIYMDKQPSQEDQVILAKEQRCLLQSQCQKLYMILSQQFQKAMGNSNPLYQSTLQNLLWASQLIIKQLLEVKQYINVKQEHEPPSPNQTQYPFPIQHNLYQSQYSFILPRINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJ75</accession>
    <name>A0A8S1PJ75_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1180155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJ75"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="117"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8102528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="206" mass="24746" checksum="7EDC1A212C4E540C" modified="2022-10-12" version="1">MNKSRDWNIVDDELNRKLKQLYEIRSQLDDQSTEQLLQNKDQNQEYNSDVNYYKEFWRYYILNEMAIKKVNELHSQNQKLHELIGDIDKLQQELHIALSYRHKKKNRRTSQEIEKSYICPYEKCNKQYGSDVSLNLHIKLKHDGGNKTDREKFAKMIIEAQQNGETITDLNINIKFPPGYLDQFKNQFLNTQQNQLNSERKSIEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PS96</accession>
    <name>A0A8S1PS96_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1280156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PS96"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="141" mass="16923" checksum="A0DD763D32CD2F3E" modified="2022-10-12" version="1">MEAYILLIQHHKKTFYKLIEQLGFYFFQKPSSIYMQNDQASENIKKKNEAFKKSESFMQEQNELDILSKYEAIVPELTHDNIAILINLFNDQRVLSINHNYLAIQDISIIKNIDEIINKFQININYINQQNDRIWQQHINM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PVS0</accession>
    <name>A0A8S1PVS0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1330013</name>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1330016</name>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1330019</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PVS0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF511">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="12"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="73"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8107132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8107138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8107144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="305" mass="36042" checksum="703F910867DAE1FB" modified="2022-10-12" version="1">MINNYDISCIIESIKKRGGLFQGSIITATNINALNYYNITSVLSIYSEFKIQNASEINNLIFDCHDTPHFDMTTLFEQAHNYLESSRQYTNFLVHCHEGISRSTTIVVSYMMKKRNLNIEQAFIYVIYQRPIVSLYQGFIKQLFLTEQNIYGRITSLLNESITPYFHYNNKSNQYHLLILLKNQEHPYKSQKIILIQKSNPLILPLDLSLLVSQSSCNLHLNKHNKSITNIHNLLLLKYRKIQMERLLLTNNLFSFHLLFQYPKKLFQHLKPIVSNKRQNDNIFIFKLIIKILKLLFDLQNYQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q0R3</accession>
    <name>A0A8S1Q0R3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitinyl hydrolase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1370060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="WETMRIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000218">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF827">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="1025"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="2">
        <location>
            <begin position="1568"/>
            <end position="1895"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8108438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2630" mass="309796" checksum="4F540C8E9CA42DD8" modified="2022-10-12" version="1">MNIEELKESIQRLTQSQDCQNDLEIIWQQIQHQVGLDLIYQSGKAVIELRQYAMGWKQANKILSYIVKVCLNNKEQLKAWETMRIVMDPDKQIYKCYELHQIDVNQFSHHDFTQYEFCAELQEGQQVDALYDDEKGNVIGWCRATIEKLNEKFVWVKWHENDDKRKIVRYSMDLAPFKTKVNDEEWQWRHSLQPGDLIDCFDNRQWQNATIIAALKDEEVEYIVGFRVYDEKGNLYDSMGRRYFGWNSQYDERIRAVSPRIQKINTFSKGQYPNPYSQEDIIHDCNDFLYPENNYAIPRFSTKQTRRSIILIQMINDFGSQGLFQYILEQIQGKCTIEFIHIYMQILNNIHEMLHKQFILNYVPALQIAVQNNILQSTDNNLRNFSAQKITDILQSLTNLLKRVYPIQKRSEIIDRFDLDIAFKCFTSDFLERKIQGLKAIQELIKRTKDLYNQYGMQEYQKQAAIQKILEWLNEKKIFETLYVGSGNSHLVQRSGEFFKFLIEEKMIGIPNLKEIWNSLDKAEYENKLAIYKLLKDVSNSLEKEQLEFLTEEICKRDPKEVSKDELDLLLDIVKSNYKYKEDYIIKCCNYYWNVLKNGQLNQTLFELYMTQYIEQVTSFEMRPHKGIQIKMIGESIVKQQSASIGLKVLIKLIEKLDISPAAWEQYTRNVALTDLQSQYGIIEQIFIQIKEIKKHHNNFEEIRIRMQFIQMFYQNINTYEYRLTFNVISTLWELLVCQSECQQEKDLVFKWFSTLSNQDGQSQLTSMVEISELKTFFIEKMANNLAHLTEEGFNCIKTVLSTINKFESFGLDVIWQIILEGENEKVSQMAIEYFHQFETTVEKLFNKLQENRKQNQKLLRCLLVLEDFIDYSEVNGVGNLKSLNALSQGEELSIQIQYENSQQKRFTIKINDNQTIVELRLAISKVIKLQWDAIGLTSLKGEIKFTENGKMIKDLRLKKDEIIMVFKKQVKDAPEVQLLDGDNLSEQVKVVFAEIFSEYSTDGKMTKEDCTRFVTGCTGNPCSIEDANIQRTFEQYDRDKDQILTLQDFYDFYTDSIRTKKSTVWLNLQTLHYRNDLVRGDRVPLPEINAQQLPRGQIVQNQEYLDLLFDLLLNSNNEVQEKTWYLLRRLPPSPQFIKQMLTFENIKQPTDWDQILVSSHYRLLYSLYIIEFLMNQQDSNHLQALIDDQDILKLKDKWMSKFLQLGGFDRLLQFFKEYQGKSVNTLSQIEKEILSFLLNTFQNYVIAACAAYIPNLYKASRGIQINKPLDQVLLDIRQSEESEEFQLLVQKLKESRLGDDIIEKIENFISVLINLIQELLKCNELEQEDRQIIEHSIIIIIVILLHNQELLAKSIENIEFINIFLSGIFTGTSDTLRNLFSRAILVFCHESQKKNNQPTRIMLQQLIIMQNSQGNSAQYYELLSQLIDSAFESEESQQFIDYEQLSQQMLQNLMNYKSSENRSKNTPTDKILVGLLNLLTKLYRFIKNPIVDKIFNDCLFSMQEDIEIKCKSVESRQAAFKLLYNLGQQQNIDTIVTTLQLLSQKIPIINRWNYIPSSDMRSNLGYCGIKNLRCICYMNAMLQQFYMIKPFRYGILQANDNKDVDLQLSKTGFTYDDNVLHQLQQMFSYLELSDRVDYNPQEFCAAFKDYAGEPVNIFIQQDAQEFLNMIFDKLENLLKETVYKNIMDGVFGGKTCTQIQCQKCKATKSKDEIFYNLSLPIKNLKTLQECFDKFVQGEIISDFKCESCNQKVDVNKCQLLAQLPNILILHLQRIVFNLDTFMNEKINTRLEFPINLDLQQYTINKDLCTQYKLVGVVVHVGTADVGHYFSYIDIKNQDQWLEFNDHKIKEFKLKQMEHECYGGSSNFDYNDNDVWGTGFRENSQSAYMLIYEKVQKDQISLVFNNEQELQDHIDQFENYTIDPNIKNGLLVDYNSLKPYIPNQYKKKVNSDNQQFLLERNLFNQDFLKFILDVSEFVNENNAFKMIDVLIKFNYDLLSKAYENSLMDQFNAKIILLIQQYPNINYLDLIYFGKLNKVVDLLLVCPEIRTRKHISKLLSTLFNQAIQIEGQITEKLNEGLDQLFLMIQDQVPKNWTRYESYFEFWLDFLQQESKHQQEYLMKKNMVLYMIDFILDKSSPLQLYEKKVQMGNAYVSMNIQTQLQILSSLLKQNQQLNLNEKKLLFLPKFYDKILKNKVDDILPIILKLSYKNKYFSEIISGCIMRGMSNGDVDEFKNYLQVAKSFLLIEDNLQIERLEWLIGIPSSKQKETTKIYDNQFSDIPSFGLYGLQSLEEDYWTFASPLGWSNCLFDYFCSHRMSKNHDIQCLLILKCCLQISLENQTCFNYISNLPCVNYQYKYNEEIFKSFLETFILDTKRFYSQHSRKQESEETKILLEEYQKRLEQIHDPTKPQYDYIIGKSVEVTKTTKLLYLLNPSTKEELTVDEVSPNFNQIKSQIDDGTLHKIITLEEKVYTTYVCDNLPTGQTNEALPQQYVKGTQIHHFSVDPNSETANFIQSKAWNVENDDKAVVSNQRLAMTVKQIQLQNNTNRNLHVILEIKGPQNSCHYIPTSKIQSLMNPKIITTMFTIIKQKSQDEFPKLELNLEYKKQEPRSESYLYLSNSNEMNLELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q6L7</accession>
    <name>A0A8S1Q6L7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1450071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q6L7"/>
    <dbReference type="OMA" id="RTIGCTQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="941" mass="110835" checksum="EA462D4563182C2C" modified="2022-10-12" version="1">MDLEEQQGLIPNITPAKPLKQIRKVKYTKSTYFNYLIAILALIILALMSYIVIDITGYEIMHLTFKSSTKQLNITNQIPIQNEDQSQIDTMDYTSQIDNLLWDDANNNKENQEVLNEEFIDENFSNQDEINQIEEPSIQNQNTDINTFQSNTLEISDQQEVEDQVQILNDDQQLEQYSLLQSGSRIITPKIDDSKYNYVELPNKMKILLIQNDDENAQIYLNDFKGYWQTNYFVKCDQCKITVKEQQTQYFIQTKSDQILNSFTELSKAFASETPQLKQINYQKTSWEELLTQISNENHPLSSKYSKSSTFQLSPNQIVLIIKGKFNSDELMNGIVQSDIVLLRDKDIEELENIEIINQHESIIKWNNQKDLNSIKILYELENSKELNFIDYFIQTSLQNYIINNKLGLDVKSNVILDQNNRQLYQIQIALPFQDQMDYDQRELAYRLSKIVINFANSLQIQLINKDNELSDYCKQMIDQLQQINNLQFDYDNQEQDYMEIAKNLKQDLIEEVLHYKFLVQIVPESLKKTLDNLQSTKNMIIIVSDSQYHTEYSFLQISKGSSTQDLLFLTQDELDINKNGISYSLKEITEQTKQFIEKTEDEIIFVFPQINQFIPQNLDLVQSTQSFDINDQVEVIVDVSKKLPKINADITIFTYGQFIYAQYLYERIIMEYQDAINVGYGIDVNQNQYFQIQLQGWSDHYTNIFKGIMEIIKEKPNEELKSQLQYKFDMLPEHLKLINSKIESILIANYKKPQFGSVGQNYKMKLIGNIQQNQAQALDQMLHKALNFKQKLKQDPIQIYSIRNKEINYQLKAKDKWHLLINYYQFSQLNQNQQKLLNRIQEYVQIQSQEQHIIFEKRTIGCTQGFFLIVQSSKDLNDQIEKLDKLIQQSLNELNKEDGSNKQSKKYSQFWKKQSGKITFFIDNQDRNTNMENLELIQCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q783</accession>
    <name>A0A8S1Q783_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1460053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8111014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q783"/>
    <dbReference type="OMA" id="FFARCYW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="112" mass="13151" checksum="E7F3A00D0D037B29" modified="2022-10-12" version="1">MLNYIQPILDFSFYLFFGIYFSGPEEYELYYRIFAVCACLFYIFIFFPNKFKLFQVNSQLLSIGTGSVALASFVLSIYLSYEDQFFAYCYWSTLLISCGIGTFLVKTKYKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7M3</accession>
    <name>A0A8S1Q7M3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1450069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7M3"/>
    <dbReference type="OMA" id="MCALVED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="328" mass="38522" checksum="053C439FD77972CD" modified="2022-10-12" version="1">MLFNNPNFQEVNLTSDSNPRPSIQEIVDKYSFRQSEKKTDDKERITFGQSYKMQRKTENEKDEKLVINEQNLSNVLSDFSIISDDPSVFIGITNLKDKVLNLIQNNRESVSQNEKKIQEEVSQIALEYSMYSQIGKSKHEPELQQMCALVEDFLYQFKKSKNLELSVIQNFQSDDSFYQIKENNIEKLNIKLNTLKDALSYQLTVLKLPETKQILQKTIDNLNEIINANDISTIKTVIFKIEQKIEQIKNNEYALLELYDYINKAVKVSRSKLTDQDRLQISKIYQAIQKFGYQNSYQQALVLYEQYLQLPTKPHIDQYINKIQQQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QB11</accession>
    <name>A0A8S1QB11_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myotubularin phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1530076</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QB11"/>
    <dbReference type="OMA" id="IQKQRIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14507">
        <property type="entry name" value="PTP-MTM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF128">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3,5-BISPHOSPHATE 3-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="4">
        <location>
            <begin position="269"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="1">
        <location>
            <position position="498"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="438"/>
            <end position="439"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="498"/>
            <end position="504"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8112929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="746" mass="87727" checksum="ABC2487EBCBC595A" modified="2022-10-12" version="1">MNNPLGRSTQKPEQQTQNNQKNNRAKEFEKQLQEEERMLAQSAGIINNNTQQTNIQQQQPAYSQQQQLNQQPQSVFGQIGQMLDAPRKLFFRNTTTQQQPIQQGQRQPTDQNEPEIIQTNIVQTYTTRDIKSIGLEIVQPGEIALQELRGICSIGTQQFQGITLATNYQIIFLPDKFPKDFRKDYFYIPYTLMIKVDKTMDRKQNDANFIEISCKDGRWFKYRFLKEQNDDCNSIFNVINKNAFTLNKQTLFAFTFYKEYSNLENDYQGWKIFNIEKDFERMGINILQENSQNQNGLFKYLNNENGIICSTYYNRLVVPAKADLDCIQKTAKFRSKERIPILSYAFPINGKVISMWRSSQCRTGIGQNRSTEDECYLKYMSFQTVDEQENQYEDKEIRLRIYDARPHINAIGQQVAGKGYENTLFYRRCSIDFLDIHNIHKVRDSQTKLLKAAFNENVQFLSQLEQSQWYDHIVAIIKGSAKIAITMTKEKINVLVHCSDGWDRTAQLTALVSIMIDGQYRTLEGFMILVQKEWINSGHQFCQRSAIGNKQNNEDSRSPIFLQFLDCVFQMQQLYPLSFEFNEKLLLDLAYHHLTSLFGTFLCDSFLEIQKQRIMEQTVSIWSWIMKQKDKYMNAFYKNPQSIDQDVIIPESFTGKSITFWKGYFLYYSLESNDAYQIHPQCQYTCDNLQEMYKALSKENHVLRQAQKDQEKLAILHQQKFNQLMQIIQETQNEEIIEKLQTIKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QM20</accession>
    <name>A0A8S1QM20_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1690030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000178">
        <property type="protein sequence ID" value="CAD8116101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QM20"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14014">
        <property type="entry name" value="STKc_PknB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="25"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="555"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8116101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="587" mass="69109" checksum="B8DB37F8E4DDA6C7" modified="2022-10-12" version="1">MQQIQQNWVGKLINAATSENPNRNYRIENVLGQGSQGSVYLGKHIINNFQQIDVAIKIQSYMSEQEIQFLKSLIQYQNQYENNKQNLQNYNPSYIIRIFDFFQHNNDWILVMELGTQDLYKFIQSKHQLPIQQLGQLLKQITKSISFLHDKQLLHRDIKPENYIQIGDEYKLTDFGLITTQFRQKTTNVGTVLYQAPELISNLNNYTQAIDIWSLGCIFYEVLSGQTLIQGTTQDQIQKMILAHKQDCTLINQRINSLNCNKDIKDMIIRMMDPNPLNRLKTQQIIEILDSKFSFQKFPIINQFPQQLNIPFAQNNQFPLQYQQIPQQQQGIIMQFNQQNQSQQQQLLVDMQKQLSQQQILLESITTNFKFLVGIVEDTKNMMNEIKKGQEQQKIEINQIKNDISVEIQSIINIKLDVYQNNIISKIDKICEEQEVIKINQQKLLVQKDQDFITQFQESINDKFKNIDKFMISIQQVLQENKLEKKEFNNQVNEQFIAKQLDENFINKLTKKNNPQKKDKQQVSKNEDQESNKSNQNKPQQDFQLIHQKKRAGTSQLKSKNQQQKQGRQTEIYDDGGNDEDRQSMKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QMN4</accession>
    <name>A0A8S1QMN4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1710026</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000180">
        <property type="protein sequence ID" value="CAD8116341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QMN4"/>
    <dbReference type="OMA" id="ISEMWRE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22010">
        <property type="entry name" value="HMG-box_ABF2-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051762">
        <property type="entry name" value="UBF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46318:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_121, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46318">
        <property type="entry name" value="UPSTREAM BINDING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09011">
        <property type="entry name" value="HMG_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="7">
        <location>
            <begin position="13"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="7">
        <location>
            <begin position="100"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="13"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="100"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="190"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="150"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="221"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8116341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="273" mass="32656" checksum="0D40826A3F3426B9" modified="2022-10-12" version="1">MQLRSGVLKKPPTRNYISGYQIFYASKLPELRKEGKFQVGDAGHKISEMWRELDDDERQKFDDQFHEMESKYKEDLILYYGGDMHDIKKYKSLMEIPEKPRKPASACLVYIAEHRKEFSNENPDFNMAKVTKVLADKYNELPPKHKKKYEDDFQQKLEQYHKEIEIWQKKFAQKQEQFDKLIEEKFKRSASKQDLEYQELPPYKRGPKKMKEDDETAPQIIEKKNDKDKDNKKNTKNSKDEGKGVQNKGDSKKYLDVDQKDEHIRRKSGQKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QU39</accession>
    <name>A0A8S1QU39_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T3050002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000314">
        <property type="protein sequence ID" value="CAD8119316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QU39"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8119316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="24951" checksum="2C63F5135223BB8D" modified="2022-10-12" version="1">MELNIAPQINILILYLTLFEIFNFIGKVQLGFQRLIQLHLQNFLRPNLNFIHSLNLNVGGVWASTDYNFQSFCHQLQGFSNFTCKPTCKTLDEIQATLDWQGSFRFKFILQMLYLQSIFKQFLNPNRPENYVQPYLIFFFCSKQNDGQYNIFVRKKRVTTDQEIIMYSIQKNEKLLPFKIMEVKENNLKFLKSLKKSLFLQNQYKILKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNC4</accession>
    <name>A0A8S1JNC4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0050497</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNC4"/>
    <dbReference type="OMA" id="WEIFETF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000771">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000660">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="334"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8043843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="489" mass="57387" checksum="590B7C61886546D3" modified="2022-10-12" version="1">MTEKNIDHYSFHTSDVLGVGSFGTVFQGIDLKNNRYVALKMIAKDNLLNDPKGKSGLMTEIKIMKKLNHKNIVQLIDVLETQNNFYIIQELCENGDLSKFLKKNRQVDEKMALNIIINVLEGFYELLKYRIIHRDLKPANILIHNNTYKLADFGFAKIVDNFNPNVLHSQVGTPLYMSPQILRNEQYSMKCDIWSLGFLFYEMLYGQSPWTAQSIPQLVKNIYTQPLVFHDSINQVSDNVKDFITKCLIIQEDERLSWQDVYHHPVFAQYFVQDPKVENLFEEKELSIAMSMREQFGTQQLSINELLRELAEDENKILNIEGFSEIFLAINENLEDEEIQYIFNCIDEESNGQITCENLLDWLNKYDISLEDTYMRSGSNTVYGAPEKKQIQQGLQQNELNQDRQLIQILYDEIKTNNLNLVELFHKFATTSDIMNYQDFTNMMLNYDTTLSDWEIFETFKLFAQNKSDNINLNQFKQVLEQNFQDDYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNE0</accession>
    <name>A0A8S1JNE0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin/coatomer adaptor adaptin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0040350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNE0"/>
    <dbReference type="OMA" id="DMCSEDV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001606">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="6">
        <location>
            <begin position="24"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="597"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="597"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8043127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="656" mass="76110" checksum="EE69EC68730F65E9" modified="2022-10-12" version="1">MLFELPDKIGKAFGNGTLSIQSSKNNKLAELINSKYTEDLVEAIRILYCQYLQNKDLTLYMSRIINLLPREDLELKKLCQILISEFAAQPNARTSKSQGDVLILATAPIFKDLTNQNTSIKLNALKTLSSLRIQELTPMLFTAIRKIQSDKNPLVRRSVALSILKMHDMCSDDVQPDDYNDIINFLLKDTHQTRLYALYVIFKITGDLSILHKYYFQLIKDLLNFNNFDLERALQLLYQYSVMYFKEGDKDLIKLLDACEILLRLPYPNIVLSISKIYLQFPQETRIKKLIRTVVKFLNIGSESKFIILQMLHQFALSYSQYLKEHYRALTILQIEKLYCKLLKLEILSLLIDENNFNNILEELLYQARSNQPQLVAVAVKLLSKTTIKFPKFIKRTIKSLLDILKTGSSDTKNHVIASFCNLISTQESKLPLLAICLQNIENISDIRYKATVIKLLRLEIGQIPTIVAESLRKLSLTFSQQHENVKYEILLLSHQLLAIHQDQHIELLNQYIMTLAKFDSSLLVRDLYRTLSKNIEFKESFVQEEENVAPSLEMQKLPIGSLSHLVGMHLPGLNLPSYLNEDTSDKRKIEIQLPPQKLEQSQQQSQQQQQQQQQQPKSQQQQQVENKKEILKRVEEDVKKHYQNLDQVLNDFFTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNH4</accession>
    <name>A0A8S1JNH4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0050448</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNH4"/>
    <dbReference type="OMA" id="LEYQMIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="144"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="186"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8043794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="238" mass="27981" checksum="4F4D1BC6063D5D16" modified="2022-10-12" version="1">MNEDNMQNLLNKKQTFESKYEEIVALTLRINQLQDSVQRNIDLDDEHMTLIQLQRDQSKKLLETIQSMINELERGAKGFEQKKQVKNAKQQLAKQKDRYQQLSKELNKLSFTQIEHTQNKSQQQHQISEQQEQHYSQESLDLEYQMIKERQEDINHIQNQSLTLQKIMNELGLKVNDQDQQLDLVVHDLQQTKSNVEHANQEIKEAANSQKQSRSKYYIMLFIASMLLLGTLGALLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPH6</accession>
    <name>A0A8S1JPH6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8043981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPH6"/>
    <dbReference type="OMA" id="FGDMLEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000154">
        <property type="entry name" value="Alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="68"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="317" mass="36642" checksum="E33E13FE48708D45" modified="2022-10-12" version="1">MYWFKKSEKDIKNQRIQQAVSEEKYRMPCPLGNSKWDEEGVIQFTCLQRNTTIKLHTYRCFTKSNSTPQSVTFFFHGLNEHLGLYAHIAQALSKQANSLCVGFDFRGFGKSEGLRGWLQSKEQHIDDCTRFIQLIKQLYPGVKLFALGQSLGGLTSYLLGMNDLVDGTILITPALMDNYYNRPYLKQIALFLGLLSPTWTPFPASFPNASKNPQILEDNMKDPYINWNSTLPGTARVLLKMMREAPNTFQSYKKPFLIISGGMDQIIDPEVGQELMKQCTSTDKELIYYENMWHDCIEEQEIHEIIPKIIEWIKKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQY5</accession>
    <name>A0A8S1JQY5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQY5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="49" mass="6231" checksum="88C4E13B81143A2E" modified="2022-10-12" version="1">MKELQEEFENNGEKQRQKFLKHFYEILDNPKNDQIIRWHEHGFCYMECR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRF4</accession>
    <name>A0A8S1JRF4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SF-assemblin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0040363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRF4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="28854" checksum="6DC99FF1686F20C1" modified="2022-10-12" version="1">MNPNTPFNERFAKISDKLNSIQLQHDTSKAHRIDTICGRINGVEERIQDTITSYNRKLHTLKDEIVRLQKQIEEENNAFETQFEQRVREVAAFESRITTKLEQEIALRKDGNLKLQGYLDEKVVYLKSDIQTEGKIRQEQVENITTSLENDLPKLYDMVKQEGQDREDNDNGTLRRAGDEIKRLNEGLGNQKKLREESEAAIFEMLKDLVSRVKSEIEEEKKLREESQESLLGLLEDACNKIYRAAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTA2</accession>
    <name>A0A8S1JTA2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050484</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTA2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000808">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="302" mass="35365" checksum="7C237938983AF1CF" modified="2022-10-12" version="1">MSISNQQLVFQQIKTYQFELIYFVSLNKIKFCNYTEFSYINQLLLVQIQITCQFYCYFQYIQLQLNKVFKKYTSHTFKLFLINFQNIFQFKQYKYINGYAIRIVIVQEQTKNLNLLVILVGDSKVGKTEIVNRFVRGCLAKQSHPTIGVEFATKIITLSENEQIKLQIWDTAGQERYRSITSCHYRKSLGAILVYDITNEQTFESLGKWIADIKSQTEENLVIMIIGNKKELVDKNEDQRKVSSGYASDFAQQNNLLYEEVSAYSGENIENSFIRLAQEIIKQKSQKPVEKPENAKEAKQMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JV94</accession>
    <name>A0A8S1JV94_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0100457</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JV94"/>
    <dbReference type="OMA" id="MSKGSKW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="670"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="870"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="975"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1182"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="605"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="623"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8046562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1248" mass="148454" checksum="59C7B39D2F89BFD7" modified="2022-10-12" version="1">MKSQYKQLIKKLFLTKMKNQVKQDLLISTAILLHDFNQMMISLQILSQLKKLKENKLFNVSFLYHFMNQKDYKENEQTKIRIMLSKIEQVQINLVLQQSRIKFEANLGTSLRAAVQTLIRQYLNEFLISQSNQQELIAGMKECVKLKIQFYEILDSDSGFTISHLFKKVKETSKFLLKIEKQFYSLFNLSPDLSLQKTFCFFEAQIMNNLLAAFNIKQSEINLKENQIKYRGIKNLNLSAEISSYILLSVDENFRTFSIIQTSHNFFKLFGLTFDLTTYNFSDIIPQFYQKHHENSVQNFFHTGQNKFYRNFNYTFIKSHNGLLKQVSLCIDNTQIFKIDKFIFVSFLQSVNTDLPIIVVDAQTQIAYFTSQTLSAFGINEINQKQFIEDGALHQVGILTIIPYFNMLSKAEINTDIVTTFCFFLKPQYSQEATLTQSSIKKDQNYYIWRNKDNINCFSIEIKIEKVTVQLGFYYQLEFISLKQILSYDERKKMLELYKPCIGESTMSKGSKWKGKHLKIMSLLSFSHFSQERKKNTLKIQDLKLKSSILLNQSQGQMKNLNQSSFIQQNQSQSNLLSSRARNEQEFYKSFNKSFVESDVQNSQFEKEEHSPDKKKNNEGKNTKINNQQLDRIGQQSSSVAGFQRPFFYNRYYLLQKMTEKRNPKKIDSFLFFFIGQSLIFFIFSFLVMINMTSDMIFIIDNIEMGSLHASIMGPHDLFFSMRITVSSYQQMQREGFLSQNQVVQLTDPFYNNIRQGYLELKESFYRQLRNVYLQEFFNDIILTLRFMDENERELQIMSQSFRESLLTILQYQYDHMRTIEIRESTSGKACQIFLMANYFVLHDKIEEITQEIQRWLLDTKEIIDNKGRIFWIILQILLITSGLIGLFNFNQYIKYYDQFLLIFNEFNKQLIYDQLNRYDIVQQQLQQNSDDIMRYNFDQELNEQEKTQSQYGRGKVHHFKLLNRNRQHLSRFKVYSIIILITVVYLIYTSVIFYKQQTFLSKYKDTINLYKIIQDLKLKSGSIYLFKEISLRWDNFTFLSQNERDILYQYIDQSHNIIDQYILLSSTYDSSQYLVGNKFTDKFIDIQQNNICLEIIDQLEDYMLFYCDKSFEGTISKGLIQTLNYISNSIRMQQQINNFTKRIEIELYEQEGSQIITRSFFGLSNLFLESVKDTCNELNQFLIYFSLIYLLISAFLIIFIKQIYRKYLINEYHQIRQSLFMIPKESILYDESLDRYLREIALHQGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0U4</accession>
    <name>A0A8S1K0U4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAC-A/B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0060140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0U4"/>
    <dbReference type="OMA" id="HPQEDDA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22054">
        <property type="entry name" value="NAC_NACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14358">
        <property type="entry name" value="UBA_NAC_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016641">
        <property type="entry name" value="EGD2/NACA0like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21713">
        <property type="entry name" value="NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAC-A/B" evidence="2">
        <location>
            <begin position="51"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="192" mass="21858" checksum="4360C8AA6C4F867B" modified="2022-10-12" version="1">MAEEKEIEIKEEKEQTQEHHHDHDHDHDHEHSDDGHDHDHQGGDDKDKKANRGEKKFKKSMLKLGMKPVAGITRVTIKRGKQFLLYIDNPEVLKSANVDNSYIVFGEAKVNDPQSQIGKKEADNLAQQVTKQVEEKHEQQQAQNPEQISDEGIPPESIKMVMEHCKCDRSKAVEALRKSDNDTVQAILTLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K137</accession>
    <name>A0A8S1K137_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120416</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K137"/>
    <dbReference type="OMA" id="QKEHPQS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="241" mass="28304" checksum="0341082289C33538" modified="2022-10-12" version="1">MRSSLALRLRNSIDNIQYDNKYSVHVNTQHHVLSPRKSVDKTTIHQIYKVTQPHQIKNKTIKLNRDVSGYNIRYNSSVDVSLEELKKIKKLEKSLYYIQENRQKMDNKINIGKVLKENPFQNSYNLEKQSKRYSLPVLNKKQSPQHNNNDKMKVTILPAISENTNAKQNNFSIEKCLEDLNKIKSGGQKEHPQSTERILDLLLLNTCDLKKIFKNQNQKNKRKIIVQSKVPQDFFNNLKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K185</accession>
    <name>A0A8S1K185_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K185"/>
    <dbReference type="OMA" id="KQIICHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="209" mass="25005" checksum="BC7D9188CD8AF3E7" modified="2022-10-12" version="1">MNSRKSSTSTLDSEKTSPRLQNIRLKPIKFIGSDFMNNLFKKPKEQEIVPIEVNSDSDSSGSEHDVFDNDEILLKGIHLGDYDKLSPTDQLQMLYKIRFETYPKRREYISEQELANETQKLVNKIKLHFYKFKKILPLQNLPDFTKIYDINQDQNYDQFWDYYRNRNNHRKHKQIICHQKKKSEPSNPVLRSNSRIRTDCRIRTLAHKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2X1</accession>
    <name>A0A8S1K2X1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0140085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2X1"/>
    <dbReference type="OMA" id="TNDQEIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="12"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8049629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="38960" checksum="DDEBE61D1D715B5B" modified="2022-10-12" version="1">MGATCSNSCCTNDQEINSKGGNDKQEVVLSKNKSLKESQQRNNKETERFNADNDQGKSDASIGSMKKENLLIQSTIGKMQRVQLEEITFSDGTTYKGEWMNGLKDGQGVLKWPSGSIYSGSFLEGKLSGKGKLILEDEDFYEGEWKDDKCNGFGVYMCKNGARYEGNWKNDKQHGKGKEIWQDGNTYEGFYNDGRKHGQGILKFSNGTTYEGDFSNNELEGQGTMVWEDKRVYKGQWKKSKMNGYGVLMFPDGRVFKGVILRQFKQHFQDDKKNGVGEFTWNDGKKMISNWNNGKQNGIGICTNGDRKIGYWEEGKRTKWLDEQEKKLHSESIQQLELVKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3S3</accession>
    <name>A0A8S1K3S3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0110470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="IYIVICK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF1">
        <property type="entry name" value="DISHEVELLED ASSOCIATED ACTIVATOR OF MORPHOGENESIS, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="2">
        <location>
            <begin position="918"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1326"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="541"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1326"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1350"/>
            <end position="1378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8047520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1378" mass="153651" checksum="B47BEE68DF6D4C16" modified="2022-10-12" version="1">MGQINPKYSPPSPDKLKLTYQIRNQAKPITLVFDTQFQVNEINQNDWDLVISSINMKIDKINKLQQLPINFKAQLIQSFMMNKSIETAPCITGSEEGLQELLIYLRTLNKEDLQKFIEDKQLIEQLRNLLKVECEGYHDKECLILNILLHCIQQNCNLDLNQPILNELAEILNPLHSVNFSLLLEIYIVICKNQTDEKMNQISISWMEMKRNGMWNYPFEPFIQILQLNKSVFSIMNMVRFINYFMEAHEDVEYSNKLKTLLINFGLRFLIEDVKAKIQTGYYKIEHCTYQYMDEMLLEQYQHYKINPSSRKRKLLPPDGKPVELSVCAALCADLFIDLQDPEAQKKYEYAVSEALAQIDAFLELTEKVYAKVMKPKKLIVDKKSRTKRAGSITTFLDEERSIKLRIPDLDYQKLLTQIREATLFPQVLDAFQDYLQSAAKIGVNVISQMMSVAVDKMIELRKNDPLKLDLQASEEKCTQLENQIRSYINEKAKMQQELNQLKEKLIDANKYIKNIELKLQTQEQIIQTYNSQSNTEKSHPPPPPPPPLPGQKASPPPPPPPPLPGQKAIPPPPPPLPGQKASPPPPPPPPLPGQKAIPPPPPPLPGQKASPPPPPPPPLPGQKAIPPPPPPLPGQKASPPPPPPPPLPGQKAIPPPPPPLPGQKASPPPPPPPPLPGQKAIPPPPPPLPGQKASPPPPPPPPLPGQKAIPPPPPPLPGQKASPPPPPPPPLPGQKAIPPPPPPLPGQKASPPPPPPPPLPGQKAIPPPPPPLPGQKASPPPPPPPPLPGQKAIPPPPPPLPGQKASPPPPPPPPLPGQKAIPPPPPPLPGQKAIPPPPPPPPLPGQKAIPPPPPPPLPGQKAGPPPPPPPPPMPGQKAGPPGPPPPPPPPGQKGIPPPPPSFGGANAKGPGIPQQVGKKKQNPQKPMKQVPWVVIKDDKIKSTIWEKIDDSKIKINTSLLEEQFCKVELVKVSGNALPSQKQQIKQKVSQLQAERCKNVELVISRLKISSLTLREAFLKIDTNILTEEKIAMIINAVPEKEEQEMFLHFNPQDLSTVATPDLYFMDLSTVPQIKLRAEAIVISYEWMGLYESVEAKQNKIENGIKLQQEDKKLQIMLEYSLGYGNYLNGQSTRGGAYAFKLDIMSQLDDVKSNDNKTNLLIVIILHIEADIGYSLYDQEFLAKIDYDFLCKTSISSLTQDINELKRMERVVERAKKSHGEDSSDQAGVKFKALQEQLQEKIKKLEEKNVILESKYKALLQYYCEDSKLQSDEFFTKIDKIWKDYQNALTQITRIKQQEQKLQRDSRKKMSQSCNLEALQQQNENLKNQQQLKQQQQQQQQNQEINSGQNKKDVMEELRRLQSRKAELAAKRQQKTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCS4</accession>
    <name>A0A8S1KCS4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0200127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCS4"/>
    <dbReference type="OMA" id="WQRLPGF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09633">
        <property type="entry name" value="Deltex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039396">
        <property type="entry name" value="Deltex_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039398">
        <property type="entry name" value="Deltex_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12622">
        <property type="entry name" value="DELTEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18102">
        <property type="entry name" value="DTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="45"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8053039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="213" mass="24532" checksum="574C9188BF5A2663" modified="2022-10-12" version="1">MFLNGQSFDQLIQRMKNFEDKLKNRPDIVLAKYFKIQKTNDKSKCSICTKTNQKSIMTPCNHIFHAKCIVEKIKNKQLKCPDCDKIYGVPAQPPGTFNKQIIKQSCQGYENTETIEIIYEIPNGLQNGIHYRGTKQTCYIPKTKEGEELADLLKKAFDKGLVFTIGRSLQDGHKYQVQFNDLHHKTSLNGGKYGYPDATYIDRMISELKQRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCS6</accession>
    <name>A0A8S1KCS6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0180110</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCS6"/>
    <dbReference type="OMA" id="LHISVMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000465">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="172"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="292"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8051465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="605" mass="68979" checksum="FE21089B1AEC8A97" modified="2022-10-12" version="1">MNVQQVPITKPPSRSPMQTYNIAKIIKTEPSQSDRQFSAGHRIERSMLSPSFLDEGSVLVQKLPPNKLVKASGKYPEFCIRDQDTGKVIDIRYQDWRNNWNTKSPNNAKLIKAVKNNCYNEVREILLKDKPDINARDVEGNTVLHLSVMSGSSKLVGLLLYHEAQIDSLNSKLQTPLMIACSLGAEDITQQLITAGADINSQDINKNTCLHLATMNERQKIIDLLLNKQSLNLNLKNKENKIAMDYSSNQQIFLQAMQKQNNNSIKIYDARTDFGSENSNSFNEDKVGPHHFKVHALIGRGSFGEVYLVEKLDTNQLLAMKVLHKSKIQKQNLTRYALTERNVLSLTKHPFIVRLRYAFQTSDKLCLIMDYCPGGDLSSHLRREQRFPEERVKLYLTQMILALEDLHKRDIIFRDLKPDNIVLDADGFAMLTDFGLSKEGVQEHYSGARSFCGSVAYLAPEMLKRCGHGKAVDWYLLGVVMYELLVGQPPYYANDREELFNNIQKADLKLPSYISNDGINLLKALLQRNPAKRLGSGKGDAEEIKAHPYFNDIDWNQVKDKQIKMPILSRKTRFIKVEHNVFEIGSFLEQSHLAGWSFAEHKIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KD29</accession>
    <name>A0A8S1KD29_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0190150</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KD29"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001126">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002022">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000818">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037867">
        <property type="entry name" value="Swd2/WDR82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861">
        <property type="entry name" value="WD40 REPEAT PROTEIN SWD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="109"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="511"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8052373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="782" mass="92256" checksum="8714D7BA94C4CAFC" modified="2022-10-12" version="1">MITDEPTVEITKDVLQGLKIQKVYKDFLKEINSIDFSQNGQYLVACDDQVVNVFDIANMKKIRTMYNNTQEIDMVKFTKDQTHIICVTKNEPYEIWHWAFEENQILKKFSGHTKIIYQLEIYNQIILSCSFDDTLRLWELNQQQDTCYAVLDLSSKPDNRVIGAFDYSGNSFGMSFVEKENGQSINCVQLYQIRKFELGPYQVKKISGTTVIQLKFSADNQFILCVCSDGQIQILDSYLLNTLFDIPGNADRINVNVCFTPDSKFLITGSNSGYLYVISIQNAIKTQTQAGQLIAKLEGHQRKCKLVLFNPKYCCLISTCRNLVHYEFINSNLVIEEIPEEIENDKVIIQETADENKIKVILVMYNHNYKLRQIIKNSNKSSQLLITFQHLLKFSQFLQSIIIHLEECWYPPALKLKKKIKQNNLFQCFYQDINIGFITSTGQIKSTAFIYVHKTIREYLIKKICIDQKQERVRFLMHFFKIENIIKIPYVHQDYKNQFLKTNKFKNEFIILNEEIETHQSLFQVYKLVDDQIYYVKRIHLRVNFNSSFMNVFNTKDVYINIAKILASLSHPNVIRLYDWWIEMLNFQPYLFMQIEYCIYPRYISQAKNLLSYSYFYMNPMPHKQKQKHIQDIILQIIQALEFLKLRKIHLIDLKPENIMVTITVTGDLQIVLADFNPQITNQQQQCYLDYTFQSIGTLILNLILTFPGDATLRNNYISKFLNCKFEESLTLLDSWAQKVKNRNREFSFQLYKNLLLLVKSIITDSHFHDFHELKKSIQKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEE3</accession>
    <name>A0A8S1KEE3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0210007</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CPILYDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035874016" description="TNFR-Cys domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="3589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3335"/>
            <end position="3352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3441"/>
            <end position="3464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3470"/>
            <end position="3489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3510"/>
            <end position="3530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3536"/>
            <end position="3558"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="3">
        <location>
            <begin position="2260"/>
            <end position="2300"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="3009"/>
            <end position="3020"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8053689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3589" mass="413072" checksum="1D84F8487739B158" modified="2022-10-12" version="1">MCFLIKAILILLPFVNGAQKLRLQGSKPLYMDCKMIDERDNGFVGSQYFYKYLSQSGNLLNLDQITFSFWMLVYSKMKLNGKQILFGFVDGYTNNPYLNLMLYYQIEQGDYYMYVVNQRFSPEIIKLKLKQENDLYIGSWNHILLSIDQSTSNTFINLKFFSTFDYQLNQFQETLVNQKLNYKFGVHSRITNEQLYNAPFDYKACVYIANFDYINGWTTMDSEIYIDYDLELKYFLKPYKTNGLNVNDIFMNVIMRSYTNPIIYSDTIGLILYKNTQIVYNFMEQLSSFTMMFWIKPQNIISNFQFLSLTDDALQKTSVGFGISKEYYLLFHQNYDKTYLGNLADNVWVHVTAGFLELSLNYDFYPTNQKKLLRVFIDEVQVTLKTITNVIVYRRLVLGPIFNDDFGSEMIDIQDIRIFKGYGILKSQGDCQLFVGAYCAFCKAGTHYCKEQDPTDNINIYKCPAGYKESGTSCALISISNCLRQSGSLCTVCADNYLLKNGQCVMITALVSPFNCQDDFAIFCQRRNLLIMNSKSIDDKSKLCIDSYNQYNPATYRCEQISSQICLQAQYLKSCYVCNSGYYLTELNTCQKTCTANYKFQLNGVCLKDCPGRYLYQYNCLIGKSQPTFTCNTSQKCNRNEVNINYYCLKSIEQTGKVISCIQFGSIQKQVYFDCDPLCKYCYGTESYQCLGCYSNMFYSPYYTTCVDDCSTQAKFKYNNRDWMVCELECPSEYLTQEYECVTTCGVGYAKYNTICQIDTDITDNYLKTTPIPMFVDCPELCQTCTSDTYCTSCLNNYPLNQNKCVTSCYPQYLYVNEDNVNQCLVNCDPTDLVYQNNNIDGYSIRQCFKIKCGYIKTNKMYQTYQHQTNPLICMYPCDSGYYAQISTYQCTKCSTNCATCYNTATTCTSCYPTTFLQDYDCFKTCKSKFKNYINWQCEGSCSIGFTIDDKIQSIQACVNKCGQVFGTFIYSLNNRCYETAPIIGAYCINLDCFNCYYNCKTCSGPNNNQCLTCYDKTFFLSNQCVIDCGTKFYDLLNWKCVNTCPINLYTTSGYQIINTINKLVTSCSSTCLYNQFQYKDLCSDLQPIGTYCVQKTNYKLCDNCASVCKTCFDSYSTTCSECNPGSYLYNTTCSSECPNDSPYKDTINNTCVVICASYQEDGYCVAACSANYYIYEAEKQCYESGCPEGTYNQINTYNCYACALGCATCIDGASNQCITCLPDYFLLGTSTCTSICNVSPDLIQDWVNGKCVKQCPSGSFLQTLQNGYLACKDTCPVYYYSNICVATCPTQTYLDGTLCNPCAGPCSECYGVDIDQCTKCDSGYYLADTTCVDTCPSTKPYANLANQACVLSCPDYLYLAKKVCFSPCPGFLAYYELDGKKECVDQCYSKSYLSSGRCYSCNSICKECYGPINGNCLQCESPYFLYQQKCQNTCPFYTDYTDRVCKESCPSNTVIQGMKCQTSCNTGYVLYNQFCVTTCPTFTYKVGNNCYVCNSLCRTCFGPSVSQCYSCIENYLINGNTCTQTCPILYDYQISKCLSTCGTKLEVTDTKSCVSNCPTGYIKCNKKCLTVLPDGYYSDGVQCQKCNTKCAQCTSPTVCQSCNLNFYLHLQTCNSFCTNKYLYMDPISRSCVSKCPPTLYHQESFDKRFCVTNCPLKLNDQCIDSCPLGMYNKDSFCTSCPQKCQECTSATNCTICIDDYFLENGLCYLSCIVGKTDYKNHLCVQECDSSLFEYQNECLEVCPTNPVFYYHSNICMDACPISTFQNNQQCIDCDISCLTCIGPLNTDCLICKDTYYLHDQQCVLTCPNLYNEVDKTCIISCPPNLLLDQNKCVLICSQFMYSNTCLSNCPPKTYDSNFTCYDCSQNCLECNSFGCLKCENGSFLQDGSCGNFCPYYYNTILNQCEQQCPEGAFLYIDQCYAACPANTYQYQKSCLLECPQKTIQINSICYQCPERCSTCKNEYECTNCESPYYQYQGACVVACPTVLPYQNKINNICQSECPPDTYEKEYECLKECDLIIYQNKCLKHCPYGYYGNSICKPCKLECKACNDFNICTECSDNFYLEQNQCDTQCTKIKDLKSKKCVDSCSQLLYQNVCYETCPINTYQFVNNCLQKCQDGYFGSTEFKCEKCPSQCITCSNFNQCNSCKVGYYMYQKQCLDECPDKLFSNPLTSQCTQQCPNKTFIFKNQCLYECPNDYLNDTENYQCVLTCNEQQYLNKNSCYPCSFECDKCTAYGNQNCITCATNYILTEDGYCFGKCKTGYYQTSNSCEKCQHKCLTCENDTECLQCRGNNRNPFDCSCPKGFYDDPFYDNCQQCPCEECISQSECLVCKNNLQVPNCSCNRRLNEDWCITCQIGSVNIQYSDDLNSIIVYFGYLISVNLINPFQPSSCSFWFNYAEIFGENAQCYLSWDRYSVHILLEPYASVNIGDELSFKQSFYRDVNQGLCDGQFIEIFIDNTVKGPSAQTKPYVLFDIPSVVSTCKTIIIKQILLDGTAKKIQEVLSWTLHEMENDDYYLQMDAFLADQSNEFTIPIGTLASNVTYTITAKYINILNRVNFTTFSFTTLPERVPYVFLQYNPLIARVYVFDCQLTYSDMKNEFTLSIEISDTHNKTYISLQQHINPIYEVPLNESLLPKETPLLFMASSGSSVVHEQIYLKSKRIDIEFLQQNRFIGLDNQINARAFDRNIQDEVLSTLNIEYQWQCNNLFNLQPCKTEENKIMQFPSRRIADIYADSQNTTFVFFVKASKDNRWTVKEQLIVVTDFEIEEEFILNQEIPKNYVNLNDEITVMIRNNQKYAFIMQEFKILSSIKIRSQTLKFRLAGLTNNFNSPVYIYLVPGNESIAFLLNKPPLEVKFNVEPLIGESLDYFNYSIQNLQPGDKFSIYYYFENLVLQNDINFQSINNGLPLIINSKEITGSFQLPNGIIDNSISVLCQIESDQGSKSYIQQDIKVNRKNYQINKLYESFNNKTNFSDLQSIHTMTKLIEIEQQQVCLKQCSGVGTCVDYKCMCPPGYYFDDCSGNLEQYNNYNNLIINVMQSLIKNPIKNNDEFRLFSQSLLFVSTLKDINNTYTNLDCQYILEQYIFNLNSRLEKINQHSINLQYQSTANLNYSQIDIRSLENKNDLHTALKSTVFMWAKTLFTQDAGIYQLQSRLNNFLSATIELSLFGIEPDEVIDYSIDTAYLKIQRINNMSNITKGRLLVESSENNNSETEYYDIVQAIYIRNYFAFDGYFPYPLQLYPLYDYQIRLQNRKQNIQLKTYISYKFKIQNDTSNLVCLMRNSHTYEWSNENCSIHLINSTYYCNCTTLAPTTICNDYDFLFQGSPQFKLDIPNMLYIIYFVQLIILGIFYIQARYSVHEKSIDNNKFGQILKLARKDKIAVFGSKIVPISDEKHNVQNESQQLQQIDRKTRNDKFSFNNFWKYHFLTSMKYNKICYFSSFLRSILIILKWNQAIIIGEVLSLFGFTYDVSMWIILSSIIFSRIFEYFLKTQAKYFFYMKQEFILSIIKLFLFLIMISTFLFGIYVYFMIENQTNLIISYSFAILIDILFLDIILFSANKFFGQDKKKLIRKKLKEFQSIAKAQNINKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKE8</accession>
    <name>A0A8S1KKE8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200394</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKE8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="45" mass="5891" checksum="86624A2A67BCAC92" modified="2022-10-12" version="1">MKNRQRKRRKWGSYFKNILGSYQFIWKFLKKNKQIKFTDRKFYHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KML4</accession>
    <name>A0A8S1KML4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0220357</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8055015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KML4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14014">
        <property type="entry name" value="STKc_PknB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="576"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="10"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="410"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="452"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="494"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="536"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="618"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="660"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="702"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8055015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="796" mass="90181" checksum="9D7D5D7F50226774" modified="2022-10-12" version="1">MIIKVEEIEIKKRQKIGVGGNGQVYLGETTDGSFVAIKEQMKISDHEYKILQALKGKKCEHIIQVYAFEEQKNGIFTIMELAESFDIKNMSDKNGICLQMAKGVSELHQLGFFHRDLKPDNFVIGKDDKIKLIDFGTTKQIREEIHTNSTGTYIYMAPEMIITNAYDSSIDIWSLGVIFYEVITNETFFAKYDTDYLTYRAITQISQQQINNKIRKKIVNQQLNTLLQKMIVQYLDGEEKIIQQKKRIGIDEVINELEKQKYFEQHLQQMEDLYEILASFKPIDRRTFNMIFEALMDIQHKENLINQISKDDRIIQFLKSIVSLTALNNKFIELGSKSLNLLVEIKVDLTNQNLEKIKIKNTSLIGANFVRCNLSGSEFDNVDISGMNLNEAQLFNCRWKNIKIDEQHKLDGHSGYVSSVCFSPDGTKLASGSWDNTIRLQDVKTGKQKVKFHGNTSYVLSVCFSPDGSTLASGGTNNSINFWDVQTGNQKAQLNGHNGLVYSVCFSPDGQTLASGSGDNLIWLWDVKTGQQKKLLNGHSKDVLSVCFSPNGTKLASGSKDYSIMLWDVKESVFKYWVFILIFLCCFIWAFIYSYFIIFMLFVVFFIWLNKQMKKSILQGHSGNVNSVCFSPDGTILASCSDDKSIRLWNIQTGQQNALLNGHSDYVRSVCFSPDGQTLASGSDDNSIRLWDVRTGKQKAEINGHSDYVRSVCFSPDGSSLASCSDDNSIRLWDVRLSNSISSNINLLLISQQPIFQAQGAQIHKGQFENNQGVDLFSFLQSKGSLISEDEIQNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KS27</accession>
    <name>A0A8S1KS27_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0270111</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KS27"/>
    <dbReference type="OMA" id="RIAIFFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="5"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8058229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="591" mass="71309" checksum="0AF9587E6EA8A9A0" modified="2022-10-12" version="1">MNYSITILYQALTTSSGKVFLIEEKSYNTSIKYYAFQFQQKEIIEKSLIQQTKILQKSKYQHLFRKYQKMLLKNQQIEQELYLFDYHEGSQFLDQKVLDYGKNKKSFENPEINQYIKQLLLALYELHKNQIPGRVFSVYNILVIQPNDRLVLMDFGFEPDIQQDQLDILAPPEYLEEIINQKQKNNKFDLKFDSWLVGAFLYHLIKFKSINQIEVSKNTYERFKYDKKEKFYEYLSKIQFIPCQTQRYKESLLSFVQSLLTCDPNKRLSFYEIYQHKYIQELNIEGVYQCIEFYKKFEQIKDPLNDVFITLDFKPGSTNLNPPDTKKKIQITETTIDQISLNCPNYLNIIMNKPQFSNPSFQDYWLQIQLDCLRCYILAYTVDIIQTTFKKYNHPFEKLYAYIIKKMHYLILWEFNNYLKSDFFKNKSDQNWSTFKEQHQNDLFKSDLINSYLEKLVTELIKDFQQIKLDQDPPLPEIIKKSLKDDKDQNKNMILNQLEFYSNVYLEALQTLLQFIIKQIINNQINQQITKNQEQLDELNQFKKLVYFCVDINYSFQPQSFQEQFKELSQQNQQVQPKDIIQLIQTKHIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSK7</accession>
    <name>A0A8S1KSK7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSK7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="129" mass="14801" checksum="1117E114C2FCA3DA" modified="2022-10-12" version="1">MYQLFTSKIRIKQSQTRQKLSLIYELELHLSQTEKLEGVCDSVLSIIINLSYADLPILLTVLAQSVYYIVYVNPPTVYQKFPLKLSPKTNVNFNLNDLVNTALFHTNGPPNTLFPIQQLKQTSIFVLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVA4</accession>
    <name>A0A8S1KVA4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0270157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVA4"/>
    <dbReference type="OMA" id="RTHAKMA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="95"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="281"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8058321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="582" mass="70306" checksum="68C0375F096BD707" modified="2022-10-12" version="1">MDNRLERNVNKLMDRYKKTSQQIDQIMQYEPTPQQPVTYFIGRSRPSKINDDDDDSSRERRRQSGSLEQISRLRETLNARDMQEREKYERIRILNKELKITLKEYMTVNKELEGKLSSKDKQIRALEQENKQLLDSLNKGDLNTKEVKIKAETSEKLIEELQQQNHILKQKLGDKKQKLTALRDQLKQAETVYEEQGQNFGRELERVQKLCEDFSNEIKNQEEQNKELEGELLKQRDELINRDEQIRYFEQMYQDIRYRDADQQSQIEYLNQQINYYKQQLDQATYKQDEAYNQLAKVEKQQQQFLQTQEQEIEKRVSNYKDLLEKKKQKINEQKLSIDNLNQKITDFQRLLDNEEKNHSIQIQDYQKLQQINSELKSVIDNQQNKLLTAENRIQSLVLEIQDQKSALQIKKQKFEEFELQSRQDFRTIEQLKNQVLHLQEQLTKSQQYCRDLENDIQMTKHENDKTMDEIEIKIDHLVKQLTQSKEEIKEYKNRENELKQKIKSQEEEILKHAQKSAKYKQQIEQSQYSLKQIEEKVRIYESEREIQSRQVLQVKQDQTTTQNKLRVLEDIHSLIKMHKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KW81</accession>
    <name>A0A8S1KW81_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane protein 230</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0260121</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM134/TMEM230 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KW81"/>
    <dbReference type="OMA" id="LHIIVRV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008590">
        <property type="entry name" value="TMEM_230/134"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05915">
        <property type="entry name" value="TMEM_230_134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8057592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="11980" checksum="0CA818C7F640D773" modified="2022-10-12" version="1">MKDYSETRPLNKKRVVRSQSPPPLRIRYNRPYKTIVLSFFLLSAGILFTEQGILQYQEKGLGETYPIFILAIMLLIPGVFYSGMFLLIVLGIGGFTYDMLPSVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KX17</accession>
    <name>A0A8S1KX17_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KX17"/>
    <dbReference type="OMA" id="GYDNQNR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="354"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="401"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="498"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="596" mass="71991" checksum="091D4FD1760BF20B" modified="2022-10-12" version="1">MTENQAWGDLSSDEENEKPQVDSKAQNQQGAYKQRNNQSQQGTSGRQQKQRQEQTQGEVDQNQEGDEHDNNINNPDQSNQQGNYNQRNNQYKKNNRDYKDKRNRGDGNYRGEGNYRGNRENQKNFKNRDYNYNKDDRQDRNDRQDKNDRQDRQDRNQRRQYQERDNTGLIQQKKDQLNNYNGDGINILIHFTLNENQLKETIKEIEIKNFIVTEDQTRFDVDKENALKLLEVYKLQIELDGRIHKLYMRIGSDHEKNNYGNRGGYQKNNYQQRQQNNDNQNDFGNRNRDEKFQNKRNNFEPPKIMRNTSSRVDDNPQQTDQQQQAQTDVQQNKGDHQQQENENQEKRVDKVVGQEENNQQNITQEQNQSRNNQKGYDNQNRAYKQNYKQYNKNNNFDREQNNQNQDQDGQNKDNQNQFRNNNNNNYNNQYRGNRDRQQNRNQNDNNDQDDQNNKEDRDNQKGGQRNYQNNRGNYKNNYNKNYRGNDRDNEIDNDKQNFNRNTNDGNNNRNYYNNNDQDNNRNYNKDHYKQKNYNNRDNQGYENKQQGDEDNQRKQNYRGNQQNEIKEFAFSSHYDKQKKESQKVAIKSNNMFDVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KX66</accession>
    <name>A0A8S1KX66_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0260322</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the argonaute family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KX66"/>
    <dbReference type="OMA" id="AKIPMHA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04658">
        <property type="entry name" value="Piwi_piwi-like_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAZ" evidence="3">
        <location>
            <begin position="284"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="4">
        <location>
            <begin position="570"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8057992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="873" mass="102254" checksum="D9F461267673F7AF" modified="2022-10-12" version="1">MNIEKSSQKHTTSSNSSRSSSKPRSRSRSNSQRRDRKTEKTESTDDKQGSDQNVKIESNLPQSARGKRQVRKIQYQLLTYIKDLPKRPPKCPNYPQQFREVNLVTNNFSVRSKRNGVIVVYSVKFLPFIDPQNNNLKREIIKSQEEALREFLGPFVYYSNTIIGGKESATFNFKLEKPIKFEDQDYYLEIKKVKVINMQDLENPNPIEADKPLMFFNYLIKDFFKNLKYQEHGKTRKFHDISRRQPIPETELSVIPGFAVNFQTNEQGFSFKIDISHRIIRTTTVLQYIDQIWKEQSNKLQKEELRKYVAHVMTKQMVLANYGTYRTYSVDNLLFDQNTAEFSIELADSKGKQQFITMDQYFKQRYNYDIKNKKAPLLVIEDTKTNRKVYLVAELCIMAGIPDNLNEFTRKQIQKECQTNPKDRFEKIKKMLFDLINRQPQTGLTMQKLKEEFGIDINSAPQYVKAKQLRPPNIVFGNQNVMPQGDKENFSFNCNNQSAFIKRYEVRIAIISPFDFDCEEFTQNIHELILKYGIKMGVQTKCHYLSGQRQNYIAEIEQTIMKYVPHTTNMIVIVLNRYMKNCYAPLKKMCMSEKFGVLTQMVSTDSISKQKKGLFSIIQKLAIQMMDKVGNCLWTVQLPQKWPDNTMIVSVIIEKGKFAGMLSSLDKSYSRYYSQMSTKIVEKCLIKDIGDMMKISFLQYKQENGCFPQKIIYFRDGLSDESISHLLQYETREIILAANQLCQFGDQIIIINIQNTNITKLFREERDQYFNAPAGTVVDSEITTKNYEFILVPVYSSRGCPRPILYRVIYDTIKIPMEQLQDFVYGQCYNYQNWTGSIKLPAIVKNCQKMIKFLVEILQTEPINKLRSSFYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L077</accession>
    <name>A0A8S1L077_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase III subunit RPC3</fullName>
            <shortName evidence="4">RNA polymerase III subunit C3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0310153</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L077"/>
    <dbReference type="OMA" id="KGLISCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055207">
        <property type="entry name" value="POLR3C_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039748">
        <property type="entry name" value="RPC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22536">
        <property type="entry name" value="WHD_POLR3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase III subunit RPC3 winged-helix" evidence="6">
        <location>
            <begin position="272"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8061129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="382" mass="45104" checksum="02295C1751FE0B41" modified="2022-10-12" version="1">MHTFRTLYFSQLAEQHFGKAMGKVIHVLDQYGPLQYTDILKYTQFDDLKFLEIVITLGIKHQIIGYKIKKPQDNASEIKQLFQSDKEKKQSDDQKQTKNKQKRKGLIFVNHNAILGREYVSELLNIEKNYNIIIDYALKNGVFGIKNLISENIKESALEGLVNELFDKSLLQIVQVDQQEEIQEATYNDTKNKSDKKNQKAKKKIEVQTQKQQSLTQNSLLQINWTGLMDIVKYNTIMQQLKQIYGISSFQDQNLYKQKQKELKENLEFKWIEDKYSQKHSRVFNLLKLKNCPLDDKQICDLALLNYQDSNTILFELLTDQLIKTQIIKVNDKDKQFFQFDQKLVDEKLKLHFYKGLISCKDKKLYFGILNQMNLMQFDVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1K7</accession>
    <name>A0A8S1L1K7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0280318</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1K7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38130">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38130:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8059273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="104" mass="12297" checksum="44BF7250313551CC" modified="2022-10-12" version="1">MQQDHWPFPHRQDLISLLTIKNQKRDFIYENRSQSTMTIQGIDGTAPKLKPYQYLNKEQFCNRDDDIPGTRSRPLIRSNNRSDNKLIVDDIKETMSKVNKFSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L328</accession>
    <name>A0A8S1L328_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0320217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L328"/>
    <dbReference type="OMA" id="YESHYFD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="126" mass="15353" checksum="EDF6C57C4F2257B3" modified="2022-10-12" version="1">MKRQKQFEFNINEKISYPYTPLRMSPSFRIKFQSPTKVQKIKFIYQKDLKEYESHYFDQFVDTNRIPTIHTQRAASQKQAQMQLRQQTPNYINLNPKDSEQENDKLSEFLRIIRGKKQVRINEHQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LAL6</accession>
    <name>A0A8S1LAL6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0340308</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LAL6"/>
    <dbReference type="OMA" id="TWSLISH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF521">
        <property type="entry name" value="LEUCINE RICH REPEAT AND PHOSPHATASE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="58"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="117"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8063411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="192" mass="22465" checksum="28CA21E5CAA464D2" modified="2022-10-12" version="1">MNNSSLCILCFQASNIKNTDLPVCQSCLNQQAQNLEHFEDEIDKIHQQDQIKQDESSSISEIVPNKLYLGNYIAAKNKKLLQKYKITDILICGDFLKQKFPQDFKYHQIMIQDSLNQSIIEYLDETFNFIDQAQNVFVHCAAGINRSPAIVCAYLMKKNKWNYDKAFEFVKERRSIINKQTNFANQLNLYQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LAQ6</accession>
    <name>A0A8S1LAQ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0290352</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8060094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LAQ6"/>
    <dbReference type="OMA" id="ETFEYNY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF33">
        <property type="entry name" value="PROTEIN HOS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="409"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="442"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8060094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="61448" checksum="D28395E98629DD5C" modified="2022-10-12" version="1">MNKFKYDIKPLYIETFEYNYLDGKTEKSFTIKKKLEAQRGKGKMQQSEPLAQFNNDKMKQSNNRAQTSRWLRQSSFAKNCTINPSTPLNNGIRKIESRMEDEILIQNSRNNSEKRNIKIPKLSYIDFNISVLNGEGSDSFSKALKLINKDNILMRKKKKSVQNSKTISTQENEITQFEQLENQHPKDMMEKIGQMLAINQRNNPIRKQSKVREAFTRNLKFLDQSFQESTSPDKKLKSVIQIYFDKKQAYQEEQNKQQEITITQLSEQTKSTKNNNRMRISTTFLKQFAYQVEEESKQKQQSKSQNFIQQSIKSSQIKDKFKKKQNTIFSIQVRLIFKKMFNQIVNCVRKMKLMKLTIKEIFQNGVIQKKPYEREGSFQFFEGVEDNNLQLINFMLLKCRYYAFDINEEGKTPLHLSSKKGYQSITERLLQYGAYVDSIDQDGKTPLYYAIQSQQKHIVYLLLYNKANPWSIKNCQYKCNNPDIMHLIKVSRKIHLILQLTRHCDRDIKWQESRNNLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LC40</accession>
    <name>A0A8S1LC40_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein 198</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0350282</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM198 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8064071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LC40"/>
    <dbReference type="OMA" id="YLWIAMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025256">
        <property type="entry name" value="TM7S3/TM198-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040236">
        <property type="entry name" value="TMEM198"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31247:SF5">
        <property type="entry name" value="DUF4203 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31247">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 198 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13886">
        <property type="entry name" value="TM7S3_TM198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035847915" description="Transmembrane protein 198" evidence="8">
        <location>
            <begin position="23"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="TM7S3/TM198-like" evidence="9">
        <location>
            <begin position="183"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8064071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="423" mass="48149" checksum="02CED1083E1A81E4" modified="2022-10-12" version="1">MNYKYFILILGCLLITIQSSKCKKVINSDAIYIFDDLQTKEYKQILNETSTLYFRFCQPILKCPEISLNTFAVIINNEGKPEQQCISLINTDSYFADSFELINEEESNEGVQAEFKNTLNGFAIKYMLYCSEEQEGLEILDVSYEQDKQLYKIEMEADNGCHLVLFSKIVEFLQDNKKFLSGILIIIGLTECLMGKKILKPTLFIFGYLIGFFFALYISSELDIGDNPFYLWIAMIIAVLLGAFVGGLSMHLDKAGIVAVGIGLGVVLSLLLWNALLVQFVTSEYILYSIMVVLSFGFSVLSFRLFDHLIIFSTSFLGSYLVFKAIGLIAGGFPSEIKSISGNSDYRYYIYFTCIIILACFGIYYQYKQWGQKIITYDEIVSSMMNGNQQAEHQDSLLNDHQNSQDFIELKEIKQKSEIKGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCE8</accession>
    <name>A0A8S1LCE8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0350077</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMC family. SMC2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCE8"/>
    <dbReference type="OMA" id="THNKIAM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03273">
        <property type="entry name" value="ABC_SMC2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027120">
        <property type="entry name" value="Smc2_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="7">
        <location>
            <begin position="512"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="171"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="246"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="432"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="677"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="888"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8063662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1181" mass="138552" checksum="957E101237935906" modified="2022-10-12" version="1">MWIKEIIIEGFKSYAQRTVITNLDPEFNAITGLNGSGKSNILDAILFCLGLSKEYDTLRIKKLQELIYKNGAAGITKAEVTIVFDNRNKEQSPLGYQDCDKLTVTRQITADKSKYFINGKSETQKNFKNMFRSVQLNIDNPHFLVAQGRITKIINLKPQELISMLEETAGTSLYNEKKREAQKLIQKKEEKLKQVNEIIEAEIQPQMQKLTDEKNIFQLWRAQEAQILVLKKDLFSYDYYQKAKTLRMKKNDLQIINEQISNQEEKMRYENAEISAIQEKIQSLQEQNRNNKYEQITEKYKEKQKLVNTLEKQVQNTRRQKETIESEKIKLEHALRTYQTDKERTDQKIEIADRQLKQVSDELKEKKDLLDEQIGQQNQSEDGNIAQNGKQMIQRQINDTISHIDSNRKDLEQVTERLQRIDNHMLSSKTIYEQMQKEATNLDTKIDLLKKRIEQSETEIQKSSTLEQQLLDFKSNRGDLDQQLLEIKKQISQSQPFIFQLNLSRMKDWDQNRVYGKLFSLFEVKDEQYMKALEFGAGAKLQNIVVDDSTTSTYLLKNNVLQTHSYIIPNKEIQQNEAKKEFVQAAAQIAKENDGFAKPAIDLITFSDKVVNSMKFVFGNFIIASSMDIARKIAYHPSNVQKCKVVTRDGDIVDPSGTLTGGYTNEKAQLLPKFKLFNRWNQEYKEIQGQIDKIELQIEKIKKDIEFKEQLNRDITQDKYQLEQLMIKQRKSNQFNFQTEQNKYLNEIQDLQIEQERFKKQIKEGEDKLVELKKELQLIQQGKNTKELIQAQIDRTKKEINKLKQQIDQQKKELIENQVESQNYEQEINKCIKKIKEETNNLDKTTIALDQLINELNLNKDQFLKITEEKNLYESKNAIHNNQMTRLLEQLQERQKYLNSTVEQLNGHQNELKKLEREQHDLKQQLKQLEDQYDFIRQDKNELSQDRLSDKFRVLETLEYEKTKQQFQRLEHDQGKLGKQVNFKVEAMTEQVEKEFQSLKDKKLILENDKSMLIQNMGELDDKKIKTIEKCFLEVNKDFSSIFSSLLHNAQAKLGRLDGQSIEDGIEMNVSFSHQQKNLSELSGGQRSLLALSFILALLKYKPAPFYILDEVDSALDLSHTENIGQMLSQNFKQSQFLLISLKEGMYQNANVLYKVQFVDGVSKIDRHELKKKKQQQQQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJD0</accession>
    <name>A0A8S1LJD0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0410197</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJD0"/>
    <dbReference type="OMA" id="KQEDNHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210:SF1">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-CH-type" evidence="5">
        <location>
            <begin position="164"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="4">
        <location>
            <begin position="287"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8067850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="433" mass="50432" checksum="C0254FB40B6DB08E" modified="2022-10-12" version="1">MGSCASMKLKKIDGNIQIAAYKVCITAFIWNKESHNLFDFESNQACRKELELDFSATLSMVDNQILALSENEYVKKQVQLLRVDCEQDYVTLSGMSQKSDQRVWAILCRRPEFESLNEWELQRGDIIKLGRMKLQLLDFNYDLDALQQQRDQSDINDEEIQSQDLDPDGCQCRICFQKSATISNPLFSPCKCIGSMKYVHLHCLQVWIQQSIKVKNQQSSTQYIWKKMECEICKMPLKSTYTYQTQIFCIMQIQKPVVPYMIWKITSDDKSKEGIIQVMELQDKAEIKIGRIPDCDIKLKDISVSRSHALIKVIKQEDNQLKLILQDNNSKFGTLLYAQSNKLLKYNIPSLQKVLYQIGRVLLYIQFKDKGKSYNKQFTCYKHPDKIVVQTKINQNEQATIIPNVEKDKDNQQSFQTNNELHQEDIVINVKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LK97</accession>
    <name>A0A8S1LK97_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Growth arrest-specific protein 8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0380047</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DRC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LK97"/>
    <dbReference type="OMA" id="CEQPNIP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039308">
        <property type="entry name" value="GAS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025593">
        <property type="entry name" value="GAS8_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31543">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31543:SF0">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13851">
        <property type="entry name" value="GAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Growth arrest-specific protein 8" evidence="12">
        <location>
            <begin position="216"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="23"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="240"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8065733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="466" mass="55304" checksum="FFCF15E8A8A05658" modified="2022-10-12" version="1">MPPKPKPKKAPQEPEDEFTKMTAQELTQNLNIFRDKLTELKQKRNYIQMDRDMVQNFFSNCLSEIQELNIKIVNKETEAEQLEETHRIQLKSYLQKVKHLEYEQEKANDEIEKDGKEAHNLENDHFSKRSDEQKRQKTHLKKLQEEYENSYIHAIEKEEKNNKKTLDKSKQVFDETLKNMEEKYKMRLQKLKEELELRLKVEIHELEERKNLHINELINNHETAFAELKQYYNTITRENLELIKNQKEEIASINAKLQKNSKIIADMKAANNNIRIPLKQATEERDILKNALKQFSKHKMSLQNLQSKNTTLTEKYAELKHNSNDLNFKYDKLLREKQELEEKFERIAMEVKKHTDLQNNVLSQQLQNMQDGLEEKEVQLKTIVERTNMDPQMYQQLTIKIKESIEAKNQLIKNLRYSIHHATKAYNDSIRVYEAKLVEFGIPPEELGFQPLATITSSMPAGLVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKH1</accession>
    <name>A0A8S1LKH1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0420034</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="GQLESCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NACHT" evidence="5">
        <location>
            <begin position="1740"/>
            <end position="1908"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2437"/>
            <end position="2478"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2479"/>
            <end position="2520"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="4057"/>
            <end position="4098"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="4099"/>
            <end position="4140"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="4141"/>
            <end position="4174"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="5007"/>
            <end position="5048"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="895"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1363"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1594"/>
            <end position="1663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8068197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="5115" mass="597802" checksum="826F419DB0043565" modified="2022-10-12" version="1">[encoded data, 5115 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LMF9</accession>
    <name>A0A8S1LMF9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0410014</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LMF9"/>
    <dbReference type="OMA" id="HTESPID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="118"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="413"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="427"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8067492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="468" mass="54839" checksum="6BF20432613FD372" modified="2022-10-12" version="1">MNSQKQNFCGNSANYDSFWIKSPQNNVIIQQEEFFIVLRQNNLKMKKLKIAEHLIIYSKTKSINCLNCQLEKVKNSLVIQQGLKISKQGKYLEIFGEIDQLYDHLKKYCVQVNFLQIYKLCSVNVEKEKKKIYKIRHRVTNEEKIAKVYELDGGDMQQINKEIQILRLLKHKNIVQMEELFQEDNRLILVFDNLEGGDFAKLLNQNKFCEDNIRIIIKQILQGIVYLHDLGIFHRDLKPENILFKNQDQIEQLQIIDFALADFYCNDHKYLFTRCGTPGYVAPEILQDKKYTLNVDVFSIGVILYMLLTQKNPFIKEQYNYEQIITANYDGHIDYSEVKCSTECLDLLKKCLKVDQHKRISARDALNHPFILGTFQTEQIQFRIKTQVQLQKQQSNGSLRKIIPIGTIDSPRFSQKQSIKSLHTESPIDTTSDSPGTPKSARTENRFANLKFSKSHQWNSFRVSKFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LMH8</accession>
    <name>A0A8S1LMH8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0440016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LMH8"/>
    <dbReference type="OMA" id="DIECPKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="26887" checksum="0A793D6424FCB626" modified="2022-10-12" version="1">MNDELEKTANFFFPVDNQGMKQKFVHTRRLLKHPVQRNVSNPSTQITTSKRSSDNSGVEGQHTPPQIYGMRKRSELHTNSEDKKSNNYRIFSSNVNQRSSYPQQFSFGDQFKFMNEFLQINDEHQSPPFKQLKKLPDIFQQRNSSQGFRQVFRIHSGQSKRETMSNFVKDIECPKQQCMQQLQLKQTINKQQLRGTQTKLPNIGQPVSFLQTKQQLKMINRIFSGKRDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSY4</accession>
    <name>A0A8S1LSY4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0450098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSY4"/>
    <dbReference type="OMA" id="RMMGTQG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035829440" evidence="1">
        <location>
            <begin position="18"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="135" mass="15853" checksum="4F6A2B8243426A02" modified="2022-10-12" version="1">MKLVILILILTIFSTQAQTQLRGKNKFEQNDELIEMNNQYTPELQEYSLFAQLTKPLYETAPMDVMLLNHEMEIARMMGTQGVTLRNYSCLVTNVQGLHLIFPISYRINIVKPYYFQTAQKSVCNHIYFLIQRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LVE5</accession>
    <name>A0A8S1LVE5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0390162</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LVE5"/>
    <dbReference type="OMA" id="HETYHVE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF32">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="146"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="406"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8066574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="602" mass="69348" checksum="795723B670CCEDCB" modified="2022-10-12" version="1">MSKEKKIESSMLTFFVSEMTQNFWEQTQTTELDSQEFLIEHEFVREARKEVKVKHTLLVLGEEYLYKISPKQNLKRVPLNILHFDTIPKLPEDHILQLNSENQLQGFKLTYANKILQVFTQNSVVFLQWQAILKRKCLLSCFHETYHVEKMIGKGSFAKVYLATSNGQSYAIKAFSKQFISEQPKGRESLLNEMQVMRAIKHPNIVQLLEVHETQNSIYFVLELVVGGELLQRVKEKGLLKSDDLMKIAFNLLSALDHLHQKKIFHRDLKPENLLFKSKEDNYNIMIADFGLAAFSDQELIFKRCGTPGFVAPEILLYIDGGPIYDTKCDIFSAGVILYILITGKQPFHGQDQKAILKANKACQIDFGLPAFLKTPIEFQDLVKKMMSSKISDRLTAQDCLKHKLFAKISQQLKQDQQDLEEQQQQVLNNLANYDIEYTIGLKNNTQNSEDLVGSQQLQWRKPAYNGNVQTVETLTNCSTVSIKQIDAQSSSLFQQQKQPQQSKFSIFSAKLSRQNSFDGGTDMFKECASPGRSKLSKEKDLHKIALKNSFRFYQKPQLQVEDNQINQEQSEVSELIKRHNSSELIKMNRTDHEIQSPKKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZC3</accession>
    <name>A0A8S1LZC3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0500088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZC3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="40" mass="5061" checksum="EF1C26EA9CEF8935" modified="2022-10-12" version="1">MQNYLWINKQKRDVGQGDIKFQYYQFWFLQIQMMDVSQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZD6</accession>
    <name>A0A8S1LZD6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0510010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZD6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="91" mass="10915" checksum="C98911A6FAB2A676" modified="2022-10-12" version="1">MMLNDKDKQNDKILPGPGDYEIKFPLFDPDYIIKNNYHLLQFNLEKIALENKREYKNKKQLGQDLINFLSNLMKQQQRNYQSLGMYSSKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M2V6</accession>
    <name>A0A8S1M2V6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0520147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M2V6"/>
    <dbReference type="OMA" id="ELMLMKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="110" mass="13240" checksum="7AE81EE7E23E2A4F" modified="2022-10-12" version="1">MNESLMVAQLKQRFSQPNQLQRGYDELLTYIKSNDLSFELMLMKMKFEEKLKKNEQALKTCNFLLFIQPNSQELHIRKLNILKQLQYFKEISDELLIIKQLFPQVNLMQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3B4</accession>
    <name>A0A8S1M3B4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0520272</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3B4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="82" mass="9096" checksum="378CB0B5D92235F5" modified="2022-10-12" version="1">MRFYSLPSGCCQFMSYFSNQCCQMHQLFRNASNIRTCASCSPLDPCGLGLMQSANSTSLPNFQASSTTVRPPELPLIINMSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M696</accession>
    <name>A0A8S1M696_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0480258</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M696"/>
    <dbReference type="OMA" id="MCENTEA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="305"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="386"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8072146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="504" mass="57359" checksum="675193FAB27ABAC6" modified="2022-10-12" version="1">MLSCHHLTNTEIQMDKDDKPTLLKMFWNLLVCSFQTVFGYVCLDICVSVTLHYINKGMTQIDVAGYGFALNCVYIIVLPIGFGFNQSLNMHTAQAIGDGKHQLAKRFFNVNLYVMLMFLIPFSGILIAIKKPFSLIVEESQREQTADCAWQYLWFLIPAIILAIIFESIKIFMASYKITYPFAIIHFITLLLHWLFSWLFIMKFEWGIPGAGFAIILTEVFNMIGIFSFIQLTEYKRTIFQGIKILPDIPRIRKLGLQYCKTSMPILVHIYCAYFIFVLLTFIALSLNTPSVNAHLALQTVSGTFFRLPISLSIAIMSYVGTEMGLGDVKRAKQYVAVGIFIFVLTCALCASLLWIFQDGFIDFFTAESGKDQFADETKEVMKDSLPWMIGGSLIIDGMQGTLSGALKGINKANLVQYSTIFAYFVCCVPIVSFFAYDWGLGNGTKGIWQGFGISNLILTTLFTLSLFFTDWQRESERIINRIKRENEMQKSTIHQIEDQLIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8V9</accession>
    <name>A0A8S1M8V9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0520169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8V9"/>
    <dbReference type="OMA" id="DEMEKCS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="186"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="252" mass="30984" checksum="058A79EA890EE514" modified="2022-10-12" version="1">MLTLTQMDEIIKLTEQHMINCQFNYDFKEADEVKSKILQMKTIRDLIEREEIQEQFKLGEERILSQTKQQIDEVNQYFNQLFEKFNYQKSQALQQLWHQQKIKLQKSVFYKRQQNAEYQNLQKIMTYLSNQKEFKKAEQYQVYLKEASQDHMRRTQSEQRQTQETQQRVLKQKHAYQEEVLINKFNDQEQLIKLEMNKKLQEIEQKRINQITQLQFERNQKITQLERSRTKSIKVQIQQQLDEMEKCSFLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDM4</accession>
    <name>A0A8S1MDM4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0600012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDM4"/>
    <dbReference type="OMA" id="DCQRTYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8078368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="154" mass="18291" checksum="40600E4B8DABF67E" modified="2022-10-12" version="1">MDCQRTYLNVNQRKKNNNYPLYRLDAIFERCEDGSSIGSHLQKRIGIDIFEDANQLKKVQTKLDITNLKQNFFNNSQNISNQFFEQKKNFQQNLELEWTNSNKVLQSNFINTEDINILEVDEINYLELNASPKIKRFKTKYSQKISKAIKKFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MFQ1</accession>
    <name>A0A8S1MFQ1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0610221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MT-A70-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MFQ1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF2">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE MT-A70-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8079257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="539" mass="62750" checksum="6112A02CB399D85D" modified="2022-10-12" version="1">MNKDKNKKVKGDPNRSRSRDKEKNEVLRFPPINQEQFRAVVIEFMLSDQFDYPISGGELYTYINTIFLTKTSTPYLRNLQIEPILNQLSDAGAINLSKLAVGIYLQDFILSISKEKLGQMNIQYSKDKPKQMPTVEEWIKEEIRSITSGQTFLKIDHLLDEARNSKTNVSLLPKIAAQKKRLIENVGNISLKYEDNVVQLALERKRAALLKTENYDEEEAKRKFTERGLQICENFTRDKCRNVQCQKTHFRKILKANTQTKLGNCTYLDQCPEQEQCKYIHYILDGNDVDWQKRVQTALSNHKTMAPQWINCDLRIFDFRVLGKFDVIMADPPWDIHMNLPYGTLKDKEMKALRVDLLQNDGVIFLWVTGRAMELGRECLILWGYRRVEELVWIKVNQLHRIIRTGRTGHWLNHSKEHCLIGIKGNPSIIKGLDCDVIVSEVRETSRKPDEVYGMINRMCPNGKKVELFGRPHNCRPNWITLGNQLPGVYLKDEGIRQRFMEAYPSVDISENNMNDNLNQMTNQENLNVIYNNHIGKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MIL0</accession>
    <name>A0A8S1MIL0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0610262</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MIL0"/>
    <dbReference type="OMA" id="QIHRKHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF0">
        <property type="entry name" value="PROTEIN KINASE SHAGGY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="10"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8079319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="304" mass="35787" checksum="FC909FDF6A939A82" modified="2022-10-12" version="1">MEKLQSIPNYEIVRIIGSGAFGYVFEAFDQQRRQKVALKRMQKVGKLQSRECEILMQLKQCSYVVKMLDVFYSRSEDNKMIQNIILEFMDQNLEDIIGDHRKRKTYLESKELKNYLYQMLKGLDQIHRKHIAHRDLKPENILVKDGKIKLCDFGSAKQLKPSTINTPYIVSRFYRAPELLLGVTEYDVSIDIWATGCIMAELALLEPLFIGKSEGDQFFQILRILGSFSKQDLKYYQKVVPFDVKLFKEFPNYEPIDLDDKFDHVDDKDLFIDLLSKLLKYIPEERITASQALKHQYFQEILDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MN29</accession>
    <name>A0A8S1MN29_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0660220</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MN29"/>
    <dbReference type="OMA" id="CILLKPC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696:SF3">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_98, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="156"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="105"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8081888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="203" mass="24196" checksum="F9EB8CBEEB8E83DC" modified="2022-10-12" version="1">MNEQDDEMLVEEIMCCQNDDDIRSLVDSQCLDIIGNSLQDQLTLAHKQLFRVIKLFREFLDSSRNQFWDQKKKREQNQTLQQDFLMIHHELQLEKSRGKQIEEVNNQLRQQIKLYEGDFETIRQQSFEDIKKVEDQLVKTKNQISLYKNSLIQKFCVICLQKEYCILLKPCGHVCVCEECSKKIDQCPIDRVKVIKMKKVYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MT67</accession>
    <name>A0A8S1MT67_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0630164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MT67"/>
    <dbReference type="OMA" id="DTFFLYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="69"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="338" mass="40224" checksum="F23D9BF22FB3D30D" modified="2022-10-12" version="1">MNLVLDSQTIDLLTNYEDLLNSTLDQGQLEQMCHELIDTFFLYIEEIQNYKGEIGLELKQKQTEIDMLHLQISKQANQLEDQKSDLTRQIEICQQLELQLQFFEKKSQKSEKEILQVETELRKKLHQQNQQYRDQIQILTSVQQEIDKQILQLSREKGQLQLQFEQLQKKYEEKQFLITNLTEQLANLQTSSSFNTKILQNNDVIIDSQDESIEIKPQEKRSQYSNCISHNQINFKWEKIQPSISSIRNSITIINPTNTRCDIQSKQKIKSLAYLNCKSENQCDPYYEFFKLITQCVKLDLGQAHIFTMNFDYLFEKFKNNCIPFNQWYDKILEEIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTL1</accession>
    <name>A0A8S1MTL1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ODAD1 central coiled coil region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0630095</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTL1"/>
    <dbReference type="OMA" id="MEREMYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035253">
        <property type="term" value="C:ciliary rootlet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097542">
        <property type="term" value="C:ciliary tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049258">
        <property type="entry name" value="ODAD1_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033192">
        <property type="entry name" value="ODAD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46518">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46518:SF1">
        <property type="entry name" value="OUTER DYNEIN ARM-DOCKING COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21773">
        <property type="entry name" value="ODAD1_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ODAD1 central coiled coil region" evidence="4">
        <location>
            <begin position="143"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="527"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="34"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="104"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="335"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8080105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="546" mass="63957" checksum="2D6E8D9950DC5074" modified="2022-10-12" version="1">MSRVQTAKSQQNIRSIEEELEHQIKKSGLLDADRKNFYNNAEETKRKNNEIIEQLKKENRDLKKLQDELIANKRGASASLAKTQGAFISWSGDIKDENYWRRKLDDAKHLTKAKKERLIQLQDKLNEVSDTKFGVPEESPLMRQIRILENNLDKVMIKYNEAQSIRKTYEQIVKRLKEERVGYDNQLAAIERSLKGKEHDYEELLLLAHDATHAKELAQAELKKYEHKKAAVRELRKTYLDAKRKAIEEREKVIQKLEKREKDNVQTQVEYNTSNIPIQESSNPNVDTNIQKQKLADYEEAFHKLKEATGFRDVNDIIQKFTSQDETSKSLSDLKGEYQDKIEQLITERQKLRDHLNDLKYEGGEALTRKQIDEIENNVNNAINKCERTKLKYERVQKILVNVKAGIEHLYEKLEFFKLEGKPNIVISDEKLVDGLSQVVEKMKLVLQLIKNDASYRAQDFKQTWKDFNKEHIDQFINLNLRDNNIKPENVSRNIRVRIQDKEDDDVSDGEIEDDIDQEVTAKLKQKYGAQAKDKNAKKLNKPNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTZ3</accession>
    <name>A0A8S1MTZ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0600030</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTZ3"/>
    <dbReference type="OMA" id="MNESQTD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="21"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="461"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="487"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="515"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8078404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="656" mass="76267" checksum="DE85F7A3330443FC" modified="2022-10-12" version="1">MEDQLKKLIEGDEMRKFSDYFQYKETIGQGAFGIVVKAVNLTTQQEVAIKIIKKKLVNRYDQLKQESSILASLRHENIVKFIDVKETDTRILIIMELIQGGSLEDLMQKLHKNGKWFTEEQCKTIIRNILQALAYMHKNNVVHRDLKPENILVNEDLNCVKLSDFGLSSVQNQLMTKQCGTLIFMAPELLMNKIYSKNVDIWGVGVIMFMLLNNGQHPYYNKGDTLEQILQKTKVMHEQGNLTQLQYSLFKKLTELDQTKRYRADQGLLHPWLNDQNDVEVPQTMEEIFHTWIQQQKLLNLIKSVMFLSKLGYMKKLENREICNKYSEQKSKLKEDKNKKEGKLTINKEFYDELFEDYQKTLTEQQKRLVQTSHQVDSEKQIKQQVQSQQLSIVQAKLQFIIRTRKPSTSSKNMEEPINDFDNEEIKLEMNESQTDMILQDNELQNDQSVFEKSDQNISMKISTNIKSPKKKRKSPLKTQTKLYIQKNDSLNNSQQGIKEKPSNSQVPSPTKLINNSVVNSNNTTKHQIKLKPLQNSNDPQKEIITNNTILNQNQISYEPNFRSVIRPVRQTHVPRLSRLSVDRDQVQPTVLDLSSMAMGGFSPVHSNQFLFPTKIQNYCTQKRAYQNEFHPSKVLTSLASKKSNDHYNFINGGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MUZ1</accession>
    <name>A0A8S1MUZ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAM50A/XAP5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0640072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MUZ1"/>
    <dbReference type="OMA" id="HKGGTVQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048337">
        <property type="entry name" value="FAM50A/XAP5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007005">
        <property type="entry name" value="XAP5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12722:SF0">
        <property type="entry name" value="PROTEIN FAM50A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12722">
        <property type="entry name" value="XAP-5 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04921">
        <property type="entry name" value="XAP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM50A/XAP5 C-terminal" evidence="2">
        <location>
            <begin position="160"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8080645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="295" mass="35656" checksum="5720F754F721CDF8" modified="2022-10-12" version="1">MYKDLDINELEKLKKEEAMLSLRKATTNIKERFGQSNQDILEEEFKSKTIGLVTREEFKRKRENIDRIYVQDLKIKQEEEEKKKMEQKLKRKQEYKLKTILLSFDSEQQELTEKKNYGKDSSVDTLYLPDMNREKKIEELTRIFIEEYQKNMELQKDQLIDIKFQYWDAQTCTRTLRIKKKTTIKEFLELARREIIRDFGFLTEFSPDDLLFVANAMILPHKISFHDIIAYKVKNRSGQPILSFEQRKVQAKGIEYEIEVENSTTCKIIEKFRYEKIKHIYPCSKWEVVEINKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N028</accession>
    <name>A0A8S1N028_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0630279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N028"/>
    <dbReference type="OMA" id="QDGKMLY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024453">
        <property type="entry name" value="Peptidase_C92"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47112">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47112:SF1">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05708">
        <property type="entry name" value="Peptidase_C92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="69"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="363" mass="42550" checksum="6CE2F40B41827125" modified="2022-10-12" version="1">MTDLRKRSLSLQNNEILCADFYQRSECSPDKLKRKSQRSQQAIIITSPTKKVEKHNSNLFEIYIDGNQTVKEGWLYQSKKVYIELTRQSIKEFNDEQKTQLIKIVSLKEFEYNIQQDKQIIKLQFPHLNQIITYQAKDAVEAKMWVGCLNIVLDKHRNHPKNKLQLQEVSRYFGFNVISEEYFLKIVESGDLLLFETNNTGAKLQRVFTNTKYDHVAIAIKMANKYLFVFDANADTGVTFMEWSQFIEINDLYEKLAIRKLMNVDRIEIEKKMIEFLQQVHGKKYEVTFSKLFRQKSLSPSKSNESFFCSELVAKAFKYCGLLESDKASSSFLPVQFTKQLKLTNQIQITDDIVVILKKHLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1M1</accession>
    <name>A0A8S1N1M1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0690108</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1M1"/>
    <dbReference type="OMA" id="TDWVFNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="4"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="335"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="350"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8083095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="440" mass="51197" checksum="EF2523941D03C7EE" modified="2022-10-12" version="1">MKNFTVFEKLGEGSFSTVLRVKRQSDQQEYAMKKVRMGQLKEKEKENSLNEIRILASISHPNIIAYKEAFYDEQSQSLCIVMEYADQGDLQQLIQQHIHQKQYIQEIEIWKMIYQMAIALRTLHQMKILHRDLKSANVFLHQGNYKLGDMNVSKVAKKDLVYTQTGTPYYASPEVWRDQPYDAKSDIWSLGCVAYEMAALKPPFRAQNMEGLYKKVQRGLYERIPPKFSSELMAVIGLCLQVQSKQRPTCSQLLNNPILLRNARQFIIESRVSQQTQSSQAGSNILLQTIKLPKNLKHLKEKLPKSKYFIESVNKSYDETQANISFLPKINNTKEIRPNFSVPPIKSDRNQQQLPQQNSLHEERSKSIVNSVQLLEIERVRQKKLLEKQQNLKISIIHQNAIPYINSSSSQIQNLYVNKQHNTRDTSKQYIEAPKPIWWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4A5</accession>
    <name>A0A8S1N4A5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0800125</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4A5"/>
    <dbReference type="OMA" id="WITQQQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="137"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="139"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8088177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="230" mass="27487" checksum="D20A87E2890200F5" modified="2022-10-12" version="1">MQFQTIKEDDMKESSYLQLKQQVNESKAQKAIQEMKTLQHIWFSWITQQQTGQSLTEEIIKKNDSIDVECTQETSTVDKNTLLKKFFETLKQRMIDDKIYSTFTQTQTTQFFEEVTTQIQNKIKLYEDIQKLKLLSNYPINTKRTYSKSANMILKKWLIENYNNPYPKQQQVEQLVQQTNLTNKQVLNWFINARNSLKNKSSQEKKFKQVVETKFKEIAIQKKKKQEKCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5A9</accession>
    <name>A0A8S1N5A9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0810170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5A9"/>
    <dbReference type="OMA" id="QMQINIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="106"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="431" mass="52339" checksum="61D43C855E15E174" modified="2022-10-12" version="1">MRQSKICQQPECKRILNSPDEKGPRIRCKDCHTKSFEKSISDIQQKKIELLIKKEQLQKEFQDYYNENKQKWDAGVEQSLNAIKIGLQNEKYQKLQHTINIRKRTLAKQQEELDDHKKLIDKLQLFSKSIQEKYYNLKQSLQKKQNEIQKKEQQYKFRQYIIFAQLPFLFDNHMIYNQYKINEEIQEIVYDTIQELVLPMRDDILISTSFFNIDYEQLRLSTTKINTKNGEICLTLKKSPVQWKEYFHDNAEYYENFITSIIKVYKLIYYMGNLFNVVLPYVIYIDNRGYPQFLEYQIFTYKRFSNLMDLKESSLSQMQINIYYLKYYFNLDIQDKLYSFFDIIDLLQSYYEKFGFQKYDIFKNLIQDYIPSIKNPINKQQYSNQQKRKPIPIKLVQTQCPKFLLQYQISEQQISNKMDNSSEFSDVEFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6C4</accession>
    <name>A0A8S1N6C4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0800230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6C4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="152" mass="17378" checksum="E7D449570BBC1BEF" modified="2022-10-12" version="1">MINNVEQLLWSQLNIFQLIRCLILCISLRRISVVFPLTLSVAELGVNAQTTLFVPFNLSPLHAFCITSTFSPGISSPQAELFIYSSISSNYIDLHDWVCVVQPIGQRRYMYNLLLRTIQVVQLGQCQRGYIQFHRFYWNCKLAVNGATVFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ND39</accession>
    <name>A0A8S1ND39_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0720203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ND39"/>
    <dbReference type="OMA" id="CKRVERI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8084674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="132" mass="15135" checksum="7D903ABE44093880" modified="2022-10-12" version="1">MGNGQCCKRVERIETLEVDNTPPQQSKLGSLFDAPQQMISLSDSDEDYQPQKQPQFGVFKEHQKSSQLSTGQQFSFQTLPSEQQFSNFVTFQAIPSQQGIKNNVFSKFQKEMNDIAKNVPQHQKNYKKNDKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NF44</accession>
    <name>A0A8S1NF44_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0740074</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NF44"/>
    <dbReference type="OMA" id="NFKHYIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="14"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="74"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8085314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="267" mass="31354" checksum="302798AAF6290AC5" modified="2022-10-12" version="1">MNRFSIQSNDMDKVHSFFGNNLWLGSLKAAKNSQLLIQNNIKTVITVANNISLKLENFKHYIFTIEDSASFRIIDYFMQINEVIEEELKNGSVLVHCVAGVSRSSACVIAYLMQSQGWSYEKTYYYVKEKRLTINPNPGFKKQLIQYRNQLNWFKKSKNSLESIQSNKILLTPRNIQKSREDLIMQLIQNNIKSPNRRIESLNQSSTTEDKEIYRFQLAQKLFYNSLKDNNKKIDQKNYQQNNINSSNINLSLYNTIHYFVKSDRVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJ65</accession>
    <name>A0A8S1NJ65_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0910133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJ65"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="153" mass="18134" checksum="EEDA1E782BC584B7" modified="2022-10-12" version="1">MNEDREFQKKVQVAVDSYLGKRDKQSQMVEQIQNVLEREQLIDQVQKLLVELITKMTERFISNMKNQVENEILKLRQTIRSTHQRTYSAVDKDILEDQVQRTLNKHDVQINMLLEHQAQLKQLRQEGLSNESIIVNINQSKSGKQVLKRNRNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NMA9</accession>
    <name>A0A8S1NMA9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TmcB/TmcC TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0850130</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="EINCACI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057352">
        <property type="entry name" value="TPR_TmcB/C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25474">
        <property type="entry name" value="TPR_TmcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="999"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1196"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1307"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1510"/>
            <end position="1531"/>
        </location>
    </feature>
    <feature type="domain" description="TmcB/TmcC TPR repeats" evidence="3">
        <location>
            <begin position="489"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1048"/>
            <end position="1091"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8090165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1571" mass="183351" checksum="ABA69612F01A73D6" modified="2022-10-12" version="1">MTSSSKTDQIYLMTLFKEIKTSLFKVFCLILKEQEQTFTQTVLLSTIQLLQLLYIPFALKIKYIWKNNQVTDVINKILSYFEILLHVKDQGFSVFLYLFYSFSGLIVLIMLGLTLLVNQQNRKNSTNWIVLVVRISIKLMITILFFPINQIFFGLLACTNNSNGISVLVYQTNIICWSDEHTIHAVLSIFIILLFQIQINTFTLFLFEAKKSNTDVFAQRSGRQFSLFHIYITLSTMVYQLVETPAYSIIIILTMFVLSLHFFYQIKYSAPFYNQYVQKLWNVITAINCWTTLMINFCFFLEGPYFQNSLLGYALGIPILIGIIFKQQDQEIDLLFSNLNQAQQPNELISLCGYLLTLNEKSIKDQHSQLLLDAFLEIHEQTCPRLDCIMKNKQLLRQIQNKHQNRQIENKIIINELIIQMLTFGIKMQPLNIDLRLYYSFFLIDEIKSPQLAFIELSQAENNSIPIDQQFIIYRYKKLIEDVQVENDVTKLDTNNFINNQNIQISLNQKLEAQCILQLEFLAALEDEKPDLAKIDSVGFKILRLKKFIDKEWQKVQQQNVYSPTLFKMMSKYFFFVLSDEYEGNMTLMKQDKFIKLQINDLINLEQASIESSPIIILNTEQNNFGIIKDVNIASCSFLGFSKSDLIERKLSVIQPQIYINYHDQLIDNYLKRITDIQKSKVEKVIFIKNKQGYIQQCSLQIKQLMTQNQEVFLIGKIGIELVYKPICYLICNTDGIIKEINCACIKLLRLDIQMIKASNKNIKDLFPDIMSRIPEILGKKNSIISFNINSNQLNPQRSSSIKSSGMDSTLLDDCVMDESLKLQCQIQEIYIDLPNAGLIGYVFKFEKITPPSIPTIHNTMVDNFYKFNFKFNSQLDTYFGSYQNYMQTLHKISYDTSSVVSSQEDQQLRQVNRKQTKKSTSISNGIKTVRLINGMLIDVEGLSEEEISESEQNELENIKQETKDSDSVIQSDYIFLKTKSSLTSMIKSSIIPQFLNSLRYLISILIVGLLTLGYLQFFFDLNYQDKIKNGLDLFYQSNQRLSKILIVQSYIQELKFMNLKYENLTSNYNFDELKEANIKIINEITNLQSKLSQASFDLAPQYISYQQEYEDSAIEMNQSNVVINYTFNEAIEQTLVKALSLSQQDLSSYTDNNVDLNYFETNSFNSIVYHNYVAQFYYYYYMVTVLEEKSQNVQIILILVTLILFIIYVACSILILKIKNLRDSILVLLLQVQEQQIINLKKNYEYFLQLIQSKEDEENLDYEEELRKPIQYQEEGAQDSLKTNDNDEAQFLIKKKRKKFENSHTILRNILISIIFIIAIFQAYFIYLIIDNNTLDSRYNAILPVLNLTSIIESYLRFGDNSFKQKYVNNDFLINFGNFNSQDYIQILKNMVSDIHKQHSENSNVLDIGYIGIFEQIFILNPCNLIKDIDSSVSLNECESFNDGIINQGLSMATIKYIKYLEQALKNPIYFTNIRKTQLTYFKDSFQYLGDKLIESISNDFSVNQLTKVSLFTVFNLLMLFVYFFIWIPLIRNLIQSIIKTRLLFSLIPNNDLLKIKAIMQYLLQIRNPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQV3</accession>
    <name>A0A8S1NQV3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0880091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQV3"/>
    <dbReference type="OMA" id="ICIEECK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8091485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="41814" checksum="9161B0860E584464" modified="2022-10-12" version="1">MTESIQFRGPLEVEGPDDLDDDDNYMRPGLETTGLQNRKQKDQPTKKSLYIAIICFFLGLFYFYFTYEQPISIHSEFATQTTFGCNRLYHWTKQGCEPNPIGCNLSVEKSSSCMICESTYYLSLQGICIEECKESNGQFCTSKNNTIISNIYKPKTNTYSYFEIPYILIHSEHSTGLLYVSDVELFKLEPYLSLNYTIVKFEDYYLLNNEELHLAFKEFQTFIEKTLLDGTLIKKSYVAFQDKSQGLLFYLASQNITDHIFLVQPTLLEEKQFYDNTYEIKSFDKLFANRTFNVTVLHTGIVPDELKCKKNTLRLMPELDNLDEKDNSNEIESTKIEEEEVDCFKIDESQIIEKIVHPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NVP5</accession>
    <name>A0A8S1NVP5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0990118</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NVP5"/>
    <dbReference type="OMA" id="KYLIITE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="558" mass="66528" checksum="B92A8CC52120D9E3" modified="2022-10-12" version="1">MLEKKSQTKDRPTLKVQERNSKSITKWNQQENKNSLNSLPTLYNVEIQKTYPKFQSRFKSVKAKLYGETENESQQYQISELIRLHSQLESINNEEYNQKAFKPLFDYFELMNCQIDFLIQIRIENNQLSAYNGQLYYPQIGIELENTTKISLLSEKMKDCINSQKIILEKLFQEINQLEQQKNEIKQKIIPINEEILPLQNQLKNVENQNGFLNQQQHKKFFQLSILQEQFNCFKRCSKQICILVQKQKVYDQNYEPYYSIRNQKYLEINTKFDQQILMSENKKSQALQQKNGNRFDQIIHRKQFVNEDLNQDLLRHTNVFFLQILCKSIKNLYKSYPFNSNEISAIFDTQRVADDQVIKINQALNQDKKCPENQDSIVVYIVRKPSDFFLQFVIKVIGCLNSFENQFCNMEDLNLQYNIINIKEDQIKYILQCSLQDYESKLNQIILNASDCEIIQIKLEIRLKLKLQRNIHFLYIIQDKITNQEAFCRQFKDCYKKKKGFKDQIGSVLESIIKNADKYLIITEMPEMIKMTCNQQKKLTQLKNLIHFSQTMATKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NW99</accession>
    <name>A0A8S1NW99_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0940064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8093803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="40" mass="4795" checksum="77E1AD07C0849DB6" modified="2022-10-12" version="1">MLLXCSNHFRLSCRISRLSTKNIRRLLLSIKESLDFSQFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWC1</accession>
    <name>A0A8S1NWC1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0940060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWC1"/>
    <dbReference type="OMA" id="MWNCASI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8093795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="337" mass="38676" checksum="DE38DE93934CC8F5" modified="2022-10-12" version="1">MYQYVEGAKFIQHLQNLDWKIGYEQLIIGEQLAQELITSTNIKGVNFNGNEIVKGNTYPVVYEESQLNDSIIQLLVINGQISIYGPSLNFQVIDHQFQYIQNEAFQNRYNLSSGTISFDDRQRIILNSEQLVTGMWNCASIFNLPQNQQFINCLLKFINQITQKGNKRIKFTKQDSFILVIFDVSATCILVSLGDPTHKLIYKPKVYKLWENFLNKNSFSSDLQFSINEYDQLESQISILQNEYSSMLKFLSEPSTHLIDSVPNKTSLLILSKSNHQTNNHNSMASHQFQGLLQVSSQKLFTGRSHLDTKRGYHLSVGQLSENSFLQDAPLINQINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWE9</accession>
    <name>A0A8S1NWE9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0990061</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWE9"/>
    <dbReference type="OMA" id="NACNVIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="335"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8095609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="498" mass="59803" checksum="E5CE845116C92E00" modified="2022-10-12" version="1">MMPSKFYKRSLSTRNEDHSKQQTIYKHPQINLTPIKKRVNLSRIDGYENIISQLYTPRQIKNIGYSPKIIKQSTYQEETQFNNIVQGYFLPTPRKISLQFNKIITEPTTFNNSPIRKQEKNCIILKNNKTDQEYDLDKQLNIPQKIRLYFSCQKLQKRFFKLQELLQFISIKSIQQYLNDQNSNTIDYVAEFDIQYKQFILTEFLCIILYFLMKYNLIKKDELGRLEKLILYQHQQNACNVIHKLQIHVPGFKTYMHLFYQLQQANPLIPKQIDLLNKYIDKTIDDLIKTIVNYQLQQQIREFLFMKIEQKTIDFLDKLKKIIEQIQEHLYFLPKNEKEYTLILDLEETLIHIDDNSQIKKRPFLQEFLEELSRYYEIVVFSHLPQLEMDKIIQQIDQNSTIKHKLNRNHLMKYEKVLIKDLTLLGRPANKYIVVDNDPFNFLLSPQNGIQIRSWYGEQGDHALQDLQPFLGDLRLYKDVRIGLTQVKTYSQYFFVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P726</accession>
    <name>A0A8S1P726_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1080037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P726"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8098840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="102" mass="12207" checksum="961A33E34DD81871" modified="2022-10-12" version="1">MRDKLTLMATQIVMDNDKPNYKTTQLFAQVPKPLQRQLVYHPKPDKYDIITNHESNQERIKKASAFDYWNPISDKKHQLFNISEIPQYRQGSRDPITWRFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7X4</accession>
    <name>A0A8S1P7X4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1090002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7X4"/>
    <dbReference type="OMA" id="FINPIHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="333"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="333"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8099149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="422" mass="50746" checksum="8BDBF7FA206F13BE" modified="2022-10-12" version="1">MTQQLSHQWAGMVQKQMAEKLLNENQQKYDQLQRKQRYKEELETDMKLKQQQKIIQHQQDQQLESQMLETLRQRESKKLKEMEQRKIKQIETSLENLNHLLIRKKMNQERSYQSLLLEQEDIKQEKLKEIKSLERIKIHKDEESRKLRENYDSVIQQKLNQKQQIKSKLLLVQETDPDQQIQIQRGDAQSYQIELSKQLEEKKRKILEIQAKKQEERLRVEQKIAFEKQKILENKLKDYNMHKQYENDNYELSNQRKQIGHNSSLQSYNIKVNDTSQQKLPQIYQDNYQAQDRNKQLRQLDQDMLKYQKMQQQLQQEQTYNPNQISNQILKPQQHAQTPLKTPLSQSSKQSKQSKKSKQFINPIHQEGPGYSQIHQSKTQTPKSKQSKKSLPLPIEITDSSNQQQRPKFKNYNILTGILHIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PE15</accession>
    <name>A0A8S1PE15_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1160029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PE15"/>
    <dbReference type="OMA" id="ISICQPF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="19510" checksum="633C3624367CA52F" modified="2022-10-12" version="1">MNSKSLSKTQIIKDQMKNQQIPYKSMERLSICQPFSNNEFFNRQKLYDDLYNTNQFKCESLNFVTTEKGIKQSKYKDIVESAKLKYAQSPNNLRNNIIQLQQNSITDIVEQLRNQKKANNVKLKNNANNLQKFQQLNLNLILIIAIPLICSLVVLFEMTRMEGQNTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PE96</accession>
    <name>A0A8S1PE96_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RCK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T1150101</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PE96"/>
    <dbReference type="OMA" id="NFHEMIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000797">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF10">
        <property type="entry name" value="SLOWPOKE 2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="239"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="14">
        <location>
            <begin position="280"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="14">
        <location>
            <begin position="634"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="570"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="994"/>
            <end position="1021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8101417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1039" mass="118840" checksum="1BE569260082CFB0" modified="2022-10-12" version="1">MNPKLSMVLPLPQLKPENNDEYENRITLRLYIEKLLNQKVGRYLEIISGIASLASVGIYIVSTYFDSVEWLATLDIVVCSLYLTEYLLKLFAAQHRLQYIFSDFAIIELLTVFPLFTIQSVENWNYLQRLINISRILRVFRIIRMINKFQSLSDTEYGGVTRQIYVIFSTVTTIIIVTAGVLYAFELPKRQDLIANDPNRGCSNQVDKCNFHEMIYFTIVTLSTVGYGDVIPQSEEGRVCVIVLIIIVLVVIPKQMNELIRLMGLQSVYARSFYKPNHEIPHIIICGYVSVASLKNFCNELFHQDHGGQDKNAIILKPSIPNTEMEDFLHNERYEMFLLYLQGNPMIERDLRRAAVTQAKACVILTNKQIVDSHSADHKNILIGLLIKKYVNHLTGCNIRLCMQLIKPESKMHYKQSLGVKMITDQIIVVEEFKMNLLAKSCFCPGIITLLGNLVTSAGEQKESLDSEWLTQYTDGMGHEIYRTDLSFKFQGKTFSEVAAIVYNEFSGILFGLELDFGKQAIITLNPGVFIIPIEYKVHAYIICQDKKVADLVATYDMTTEEIANYHFSLSQPNTSDKDKPNEEEENQAEDHLLSGKDQLDDQYIIEKDYLLLNEPVSLMDATSIFLLEQPEINNHIVVCGIHPSIYYFLLPLRAKYLKEIQYVVILAPEKPTEIWEYINRFPKVKFVQGSPLISEDLQRASIHCADKAVIFAQSSDANKTESEDFLDQMHDAESIFIYKAIKKINPSIQIMIELVSSSNIQFLLDKDYKYQNDFKYELTPLQASGEVYISAMIDTLTCQAYYNPHIVTILQQILTGMRQSNPITKAIVEDLDIKDSNLYQVPVPEDYLNKTFGELFNYLSIERHLIPLGLYRLAKAVDNKHPYVYTNPPAETTLTPRDKVFVLAHQLPADLSGIPFDANSDPKQLNNKEVENNKALNFLLEKLKQQTSSQNGVNRVVKSKNDKTVIFQGQQIKQARNIEAESMNLNSAALQVLDQVNEQISIVKDQINDIKSNLTQKEDEIVEKCRRAIRYELGTVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PG47</accession>
    <name>A0A8S1PG47_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Casein kinase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1170044</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8101934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PG47"/>
    <dbReference type="OMA" id="LPTFEQH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001472">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="404"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="340"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8101934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="432" mass="50714" checksum="449825FEBDFBE68B" modified="2022-10-12" version="1">MNKDQPVIIDNRYLLASLHYQGRHSNFYNAVDIQQEQKQILIQIITDQICICKLFKRLEIAIDYRDKSNDHLNRMSRFIPIPRIYKMGTLVVQDITYHYIAWQKSGPSLKLCFKLMKYKFSLKTITLVALKMQIHNQNILHRSLKLSCIMTTNHSNLLLTNFDDYVEFLNKDGKISNKSKQIHIKTNKFSSIGQHLQQLPSPRDDLESLGYLLLYLLSQGKLPTKCQSKDQNIRDKFYMEFKRQFLPEKELKQFPEAFLYFFQNVFKLGIKEIPNYEILKQPFIQYLGRQEQDYNFDWSEVFKPLIELTATSEISIRDKACSFEALMMNNVKKEYHKDNQSAPTSPTNQQHKKKPIKSQRFTLAPILEKNESLMTTFNNCDSNAASPRFNHQIVNLLFDNCQQDSSSGEISPSDEPTVDQLPTFEQHQHDTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-06-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGQ9</accession>
    <name>A0A8S1PGQ9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1180161</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANT" evidence="2">
        <location>
            <begin position="22"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="24"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="24"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8102540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="168" mass="20438" checksum="CD55FBC05CE21957" modified="2022-10-12" version="1">MINDFLDFCHKNDGRQINKLVSSRKKWKEVEDELLLKGIRKYGNQWSKIQEEVPTKDSKQCQARWKILKNVICAQRCLRKKKLWIDWKLWVISQRWKIKIIRQKRSRYHRXVFMKEAFFFSLKFLFNSTLGMYKGFGTFISVHQLLLQQKLITVVILIQQQCYVIGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGT7</accession>
    <name>A0A8S1PGT7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1170114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGT7"/>
    <dbReference type="OMA" id="NYAPIFY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035751133" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="224"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8102071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="42000" checksum="A37B07CF3CE6867C" modified="2022-10-12" version="1">MKIALILIAITLVSATSISPYNKLNYSKQRSMTAVLAEVESKIQANSPLDAVLNVLKQFKEAVNVEQVNHDQIFNIEYSECDSEFEFRNKEVEDASNVVRDSTSQLNVCKTSKIRTTNQQEVNQQQLFTYQEHLTLILTTAENEQGYFKKRGRDYEDSLHAIDEALDILSGIANGKRSFAELSSVSKSMLQTSFNIQQTINYAPIFYAFTQLASQQDLVDPSQIERVEQLLLQLRENVHDNYNEFTTSNAAAVAAFNDQKDRINIILNRLSNQEERLQDKLDHLNQCIGTQSAIVQAASTKLERNQQLWDQAVALCNTFEAEYFTATMARRSEISLITELEQQVIKRFQVVKEQKPARMLRYAKLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIN7</accession>
    <name>A0A8S1PIN7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DC-UbP/UBTD2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1190062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIN7"/>
    <dbReference type="OMA" id="MADSICQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032752">
        <property type="entry name" value="DC-UbP/UBTD2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039869">
        <property type="entry name" value="UBTD1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13609">
        <property type="entry name" value="UBIQUITIN DOMAIN CONTAINING 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16455">
        <property type="entry name" value="UBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DC-UbP/UBTD2 N-terminal" evidence="1">
        <location>
            <begin position="63"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="237" mass="27700" checksum="DA2BB78E62112897" modified="2022-10-12" version="1">MGSCATKNQENEVSLPSNPRLQRAYQQYSQIIEITHPLNVNQKYNSYFTLIPDQFIGTGIKKTNQYESRLSKEEWQQKRVEFWDSRIEGQKENWTTIKAALEADEGTAKTLLQAADLKLIKNSMQIVFDNHGQKYDLPVFVMHNPVSFPQKQSYDQNLYQSFDRKIVKFKVRSTKWNIDKEVSYNTGDKVESLIKELQSKEESQKMKLFVNGREMRGHNMFGNYGVQDNSVVQAFMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJ93</accession>
    <name>A0A8S1PJ93_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1200065</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJ93"/>
    <dbReference type="OMA" id="ESKFESY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="782"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="839"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8103160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="923" mass="109500" checksum="B811EBD94C231AFC" modified="2022-10-12" version="1">MDDDIFLGLETIQQFDEKMKQFSQQHQFRRQLQRYPVQKILKRSNSFAQSQISTAINSSAHASRKQTISQLEMNPPKRRHCEFYNDEFCMNLFAEKIEYLCTQCYDKNEVVIKELNRVAQELGISLHRVQQFESVQEREQYILSQLQNQVLDSIKQWKQRIDQREIQRRKSDNITINTNSQDKMLDIFDWIEIDTKFRQLKKRLKFALVEMINDKNNTSKISFQLSNRTGDTIQDLLYKKPDTRQIIDELTNLLKTIKNKRNVTNIQLNSPKSVRIEKWNQSKEEQFIAKRLSAKQRLSKLINSSSWNNIAIGEFVSNLDKGQQNKQKRQELESKFESYQSMNEDDLLYLINTSVLLNRCSDKKFIQSAINHLDQDIISDIELQIAENILKSHFDIQKQVNQTRHDFLKERNEIQFIKNRQAKNIQERQEKQNSIKKLQIKMCNILREMKDKTKTTMKQDSEKYNLYYQEYKQGNPIANPYVWKFKEIMMNVIKKKKNERRDQKLGVKSENHKPIFPSKEESLILMNVIRKTHPSSKLRLIPQQQQEYFPTPAKVFNEYKLKVQKKEYEKNQSNFLNFYTGGPKKPRKYSSPQLQFIEGGENKSDWITPEIELIAINKIKRALKAYVSRKRYIREREKKRQKEYQEKEKDPKFFRILSRMKSKNYNSLDIEECYKSLKQAIAQSAGNSKQLLQKGVQMDYSKVKVPHSMIQLKLKQRKLFLALKVGNTNWAEYSGFQFEPQDVNCYDTNMYCPLYHAAQLQSITFCNFLINAGADVNMPCQGGLTPTFAAFITNNIVLINLFISNGANIDILNDEGKNPLCYCSIQMLKELNLQYNPVNSKSQTHRQKSRRTQSNLEQNYSPYAEMKMEPMLNVNDEIKWQQDQIVSLRTFYTKKERDNFYYLKQKAQSQKESENNQENQEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKD2</accession>
    <name>A0A8S1PKD2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1210171</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKD2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF27">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="53"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8103685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="206" mass="24738" checksum="882FE240DFBA19B0" modified="2022-10-12" version="1">MQNQDFDIESLSQNLEGISNDYQQLIKDVQNILRNEIVDEDGLIKLCQQGFTNQTSRLRGIIWRLLLGYFPLNRKYWTQVIIKNKDNYEKIKKENIKKAPPQKKNDHPLSRNTDSDWNNHFQDQQLWSKIQKDVIRTRAKELGKEEYREMLNRILFLCCKLNKMDYVQGMNEFAALMQCQIDLVFICVQVIQMRNYRMRVMHFTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMP1</accession>
    <name>A0A8S1PMP1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1230092</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMP1"/>
    <dbReference type="OMA" id="QNMEKEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8104256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="234" mass="27388" checksum="F29679A542370450" modified="2022-10-12" version="1">MIPIQNFNHRQSASVLYRQYESRKQSPVTHVVVTHNPRRSHQFDGRSNSPSKILTTSYVYPKIMQSTELKTRIPTQGQSIPIQEALKIYSSKQTPDSNYNSNKVSSFLQEKLNERSNYTKTTTGESQEEERSSKHKPSIIYQKQLLDKDLQIMQLQKEIKQIKESNKKYYNIEKEYDKLLQENQKLKQQIQLQQVQITQLTHQQSLISPGRPSEYVSALSEQDKLKIKTLLECE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PNV9</accession>
    <name>A0A8S1PNV9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1250014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PNV9"/>
    <dbReference type="OMA" id="TESITMP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="55094" checksum="272D3FEB5E0594D6" modified="2022-10-12" version="1">MNKILNTIDKFDIFGVPINLLTNAKTSLYQSRLGGLVTMLIGSISLTYFLYVFILWIDHQIPPVVSFKQQTISYAEYQLSDSIIQLEFQDFLGGVDPFKKENNIITPKLYRIINSTIVDKPIPLFSSKDKPSKIFINDGTIVLNNGNTENDDNLDMTQLLLVLEGCDNLTTVSGSYCADENVITEYLSKFHGFLFLTIYLNQFQYSTGKLEQIKKQYYTAFEPSKPLYSQVMLKQQESIIDDGILFTNYQNYQFLNNYELINQQVDHFFTSQVVSLLSNQTYEFKSYGCYLFRIDNIAVTESITMPKLGQILAQVGSIVQVIFLLKYIAIYYNRMLLENELLHDIVTMYYPEMKKIKFNLLNQIEMQDNPENSSNNSLEKFKYKYKTFIKRAKEKCRLDNILYEISRIQFIIQQQFGDQILQQSHQLGGKLSNHYMDHQNSKESNRLIIKPANSLDIEKDFALLEPLEILQKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PR93</accession>
    <name>A0A8S1PR93_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1290020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8105970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PR93"/>
    <dbReference type="OMA" id="DSIICSQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="491"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="536"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="757" mass="88057" checksum="584B131358CAB6C6" modified="2022-10-12" version="1">MESQSNILRCLDHNEFVVFLARSKSEQKTIKLCHKCVIDKKKDLTLKSLILLDDAKKYLQETKSEILEERNSLNNYNIGILNDIKENIGNLALNFNLQIDKIKENIDSGIQIIEASAIKFQKELSDISLDQIENINTDKSSILCSFINYKELQSSLINQISNLFNSQLLQKCQKLVSSLNVGEQNETLNQLFNIGSFIDQYTEIKADWICDKHEKQIEFVDLSQQKSIQNRLACLECVPEYVIQYTNLGQLQKQWEKYTEQALTFFNVYQTNTSSKKAQIILQLKDFREVILKLIDQLIESIQIKSQLENDDLEYHHKLLQKPWQKLSKVDLINIVDLLSSQNRLALINHMIEQEFQTKDQAIDSIICSQMQQLQNLINSTFSQSITYTIKPFTDNEKLNKNKIDVESQIQQQFSLQSQIVNLKLQLNKQNILPQIFKPFNYEIISQISETSKCRTFAFNNDQSILITGLDDGSLKVFEFNAGQLKEIQLLKEHSSYIQCLYFMQKSSLFLSAGNDKQIIIWQVNNQKQWQSYQKLEGHTDRVNCLIMNNIEDCIISGSNDKTIKFWVQENKLWKCNQTLSDHTNKVRCLSLNISQNQLISCGEDRLLLIISLDNNKRWNTIQKISVEYYGYSLCFITDQIFTFQPKQINIMHIYELNTLSQQFIKIEKEVSIKSDADCSQLFPQKFIKEKQILLNKNGQNVNLIRLHSNGLFKTEQSIENKDNCCGLFGTISNDGNYLVTYESASKEIKVRKYREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PS63</accession>
    <name>A0A8S1PS63_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1270088</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PS63"/>
    <dbReference type="OMA" id="YIFLEKC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="300"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8105469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="766" mass="91403" checksum="206B6C19B3B800C7" modified="2022-10-12" version="1">MDLQFSFQVVNLSQNYQIRLSQPKIVNTQLYYQNKNEVNGKKLRDANLYYPCRNIQTNQNFLAQLIYIEPNKNSQQEIAVFDLFCKHPHTNIIKIFDLIEKDHWYVIQEDITLNLEEFMKIRQQLSLSLKINFFQQLISGFKHLKLHKIILRDLQPKHILVKVIRDDEYILKISDFRSAEITENGKIQSINGMSDFAAPETFIEGETLTNSCCVYTLGMLLYYICNDGKKPFQAESYQDFIQKQKEFCQTLSIQQYQNKDDDQLRKYYKEMLIYDKNKRNQTFYNEFCQDYFLLDNLYFLKRSSQVGEGQQGTVVDAINIETQEKLVCKMIQKKVLQNDEDMREIQVCEHLKGEHHQNIIKIIKIIKDPQWYFIFFEKCEKNMKQFIQENKNYLTDLEIIDFLSQIIQGYNFLKQKKIVHRDLKPENIMIKFDNNNNKIYKIIDFGVSKIVSSQDLACTDVGSLLYKAPEILENEDGYTSQCDIFSLGALMYYMMYNKEYINAKSFMEIAQSQKQLKIHPFKCPDTMRNIDIKQLIEKMIVFDPAKRISWEQLMGYKLSKDYLDILNDIYSFLLIALQTEQLLYEIQPNYKDNVMLTEDIYAHRSILLKFIELTFQKINQSFQSNSVSFNETEYKINQKFNIDSWKMDNKWNKSNQLLQQRQQKLEKKQITQSVQALNVIQNMEIKNSEIPLNFKIVHRFYMDLPNLLKNPIILQNESIQLKYHLLKMRNLFNDFTYVYILGQSVSKQYNEKISEQEMQTYFNSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PW73</accession>
    <name>A0A8S1PW73_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1320060</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8106928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PW73"/>
    <dbReference type="OMA" id="ASQFIMK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="677"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="45"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="108"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="154"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8106928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="706" mass="81850" checksum="035AD22BF0E0D19C" modified="2022-10-12" version="1">MSKNKKKQNTKNKQENNKDQTKQQEQIESASDIDKKIEEQEIKYEQNNQQELDNQETIQQNQQTTNDQITQEQPQNNLKHEQNNQLEEIQNTHQEQENDQLEQFNDAQQQKQQDQITNQQEQVQNTQSEVQQPSQQKDQDEQQQQRSSIQNEQRSVETQHLKEKIEHVTDGSSNLQSDKNKNQVDQQVNNTQGQNQHEEINQFNEPKLHSQPSPDSPNDEIMKPSSKPNPNVSLSIHENEVEHSVETPTQKPMNEIAIVQIQQHEVILNVQVPIIVEKRNDQVMIEGAPQTQLRKQNDEVVLERVQVQEQKMEKQIEQTNIEVQPKIHIEQHQQIELEKQPQMQQQVIIEKTETQSQVQVEVQKNRSEIFTEEKTQMQTENSNENVYLQQIPIVQLEHHQNVSIEQQPNIYLESRNLDTQIEICPQIQIPLNAQTTSLQEHPIVNLEINHNIHQIHPSPDVQSIQTQQFNQIEHQPHVSVEQQGQAQYSVQAPQTEVQINQIQSSSEPQQQLKVESNQLQTQLIQEEEKVNVKQEDKTHIENQPSHEITNVEKREIIEQKPLLQTEQNSNPVEVSHNDTVRIEGYYVDQKNNQFKQQKQVPLFADSPTQQIRDSEPLQPQNDAERQFLNRAQSDIHSSQSKIFQQVPQEVGALGLSVAAAAGASQFIMKNGVKSKEGLFAALAGSAILYAGYRLFKSKSQQNQQRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZF3</accession>
    <name>A0A8S1PZF3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1370082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZF3"/>
    <dbReference type="OMA" id="QYHVANE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8108482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="212" mass="24236" checksum="D46BF29075757EF9" modified="2022-10-12" version="1">MPKGKQTKQEQVKQTEVVDKKIKKGKKAKANDEPVEQPASPPQSPIRKGKKQQQEKKPKATKERFNKLYYPETPNQQFPNQLQQNVINVIASFGTSAPLKDITNNLNLFYTNQIEEQVVHNFLAQAEETGIIYERSGQYHVAQELVPPQPQPKQVVSQNIEQEALPLQGIESAVKQHLDNDQIDFEDQIQNQVSEKKDVINDNDDHQNLFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q275</accession>
    <name>A0A8S1Q275_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1410152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q275"/>
    <dbReference type="OMA" id="QCIAMVE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="162" mass="18918" checksum="ED0E5CFA1E825086" modified="2022-10-12" version="1">MEFATRPPSNCINPDSQSPLKFIRKLTRQASTVNGSSFKIQTCIQSVPNKTQDVIQQCIEIVDQIRQQHQERQGSRFFHVRSCSMPCDRVSIINQLIETILPQSPRKPQRSLEGEIQSVMNQLIEEKQYKQRAIDQRDSLAKRQDYSIQQLKSRIEQYQKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4T1</accession>
    <name>A0A8S1Q4T1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1420133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000146">
        <property type="protein sequence ID" value="CAD8110035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q4T1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025:SF3">
        <property type="entry name" value="HORMONE-SENSITIVE LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="1">
        <location>
            <begin position="4"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="116" mass="13320" checksum="E2003FA14DB573F4" modified="2022-10-12" version="1">MNDKILSHTILGICIESYSAHPDSQLGIDSFMSPLLLSYDILKYFPPTRLFCGNKDPLYDQVFRLASRLQVVQRDVKITIYENLSHGYLNFNTIKGMSEIKQCILDSQDAFQELLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4W3</accession>
    <name>A0A8S1Q4W3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1430123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000147">
        <property type="protein sequence ID" value="CAD8110313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q4W3"/>
    <dbReference type="OMA" id="RSLICIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="34879" checksum="C4B93038E54095E3" modified="2022-10-12" version="1">MKFNSSNSFLFTGSDKKIKIYSFRKGLLYKIGESYHSDQALTYLNCNKDTILISGCNQMIKIRSLICINNSKVQRKIQVDQNSFSCFAISEKNNLLVAGLQKHIYFWEENGLCVLKLDLQLIDGFDWVYSLSFNESQNKIISCNGDHNITIIQQQNNQIQQWKIVQIINMDNFGTQAICFGDDSFVFHPWMSRTMFVYKFNQQNDQYTLSNTFLMQGEGQNCYSFISQYIKQKQLFIKQDKNELCLFQVNKNQELQIIQKVRFQEIYIWGTMSNDGLWLIVWDEAQKLLKVISCFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5A6</accession>
    <name>A0A8S1Q5A6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1450117</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5A6"/>
    <dbReference type="OMA" id="CCMKGKA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06606">
        <property type="entry name" value="STKc_MAPKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001222">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002032">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF369">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="79"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="336"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8110846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="402" mass="45725" checksum="948D9027392DA317" modified="2022-10-12" version="1">MGCCMKGKAHVAASSINPTQRQTRKFESQRSRVDELKSEVFEKTMYGNEDNYYDADELTCIDRNQLLSLIKSEPKKIRWTPGQVIGQGSFGRVIEAMNLDTGQLMAVKQVMVDIRNEDRIIALEIEIDLLSLIKHKNIVSYYGMERTDKTLNIFLERVAGGSLSSMLQKFGSFQESLIKVYMRQILLGLEYLHQNGIMHRDIKGANVLVDNQGVCKLADFGSSKKIALNSDSTIFGTPNFMAPEVVQQQKSGRKADIWSLGCTMIELATGKPPWHQLTNQFAVMIKIGKGEIPQIPEGFSEEAKNFVSHCLEVDERKRWNATKLLKHPFLIQQNKLEIPQGKTPLRNTPGSKSKQKQRSFKYPEQETSHSPGQLKDFEQQHSENLQPMNDSSFVLKQKRQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5B0</accession>
    <name>A0A8S1Q5B0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1430074</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDM010000147">
        <property type="protein sequence ID" value="CAD8110215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5B0"/>
    <dbReference type="OMA" id="GMNTGQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="123"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8110215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="320" mass="36420" checksum="97EAFF354F2BCBD0" modified="2022-10-12" version="1">MSKDNKGEIDVDNIIERLLSVRGSKPGKNVNLTEAEVRGLCIKARDIFISQPILLELEAPLKICGDVHGQYFDLLRLFEYGGYPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYTIKLWKTFTDCFNCLPVAALIDEKILCMHGGLSPELSNLEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWADNERGVSYVFSQEIVQVFLKKHELDLICRAHQVVEDGYEFFSKRQLVTLFSAPNYCGEFDNAGSMMTVDESLMCSFQILKPAEQGQGASQQNKAGSAKFVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5F2</accession>
    <name>A0A8S1Q5F2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1450093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5F2"/>
    <dbReference type="OMA" id="EQIQSPR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="482" mass="56954" checksum="ADB6CA85E5DBE2AC" modified="2022-10-12" version="1">MNENKKNRVFSFQIKNTSGVLSEVLDSQLSTTRFQGIESSHNSLKPNFFFQESEQIQSPRISTQRSLSRLHINSLSPKNSYIYSRKSILTAINSQLGMLYHFEKQYAECLKSNYQIGVIESLFNLMVIAVEQDNIDLLITFMRLCALTFISFQEWTKALLFFQHCKFLSEFTRQLDVIQWTFLQIGILCKYIKKYDIGKIFIKKSLEYSWLNKDLDQEIICYEELGKLCFLNYELQTAKSLHEKSMKGTFEKEQSSLRQVSKKGMEQILNMFPNQEKKLGLHIFSKAVNFPLKFVQCQNQQYFQKRSVKNQYLISNDFECIPNQRIIEIDLTLDDMLQSFLKGVNFSFEIPIPISITEEYESIAEYNSMPSTQKMKQTQLATIIKLHSQKVRTTKNKSPIIRLDQSQQKLPLEQIIKQRLEMKFDTTYTHREKIRNRKFVNNLEQIRLTHEKNSITLSLEHQKRELIRYAQKLLNQQQITFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q6E7</accession>
    <name>A0A8S1Q6E7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1460065</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8111038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q6E7"/>
    <dbReference type="OMA" id="QMQQAWK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="255"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="65"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8111038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="301" mass="35993" checksum="9D3073F191572BB7" modified="2022-10-12" version="1">MSNILRICPKCQASPATIQCIDCQEKMCYQCEQNVHSKLKQHKTDIIPYSQMKQINSTQFINSGIQDHSNRSQELQQELNELESIIEAKKVFLAKQEEKWSTQINSLETQYEKKLKEFEKEIESNENQFQNYYQSGNTTFQIADIKKQMETQMQQAWKELNDKKDKLQEKETLLKDMIQTEQFCSQEIAQKEKLMNQYKDLLQQQQVERDLISEENQKIMKQLESIKSLCKKSLPEFGINVDFLDKLTRMNKLKNTQQVEEQDEEYEEEDQNQEEEMDVEENENKQNKEEENENQQQQEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8B8</accession>
    <name>A0A8S1Q8B8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1490060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8B8"/>
    <dbReference type="OMA" id="RRYHLNN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15571">
        <property type="entry name" value="ePHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051188">
        <property type="entry name" value="PHD-type_Zinc_Finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420:SF42">
        <property type="entry name" value="G2_M PHASE-SPECIFIC E3 UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420">
        <property type="entry name" value="PHD FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="245"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="161"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="368"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="166"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8111829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="476" mass="56011" checksum="DFD781089320DF78" modified="2022-10-12" version="1">MNLYLESAAEEFEQAISDADQLLALTKATLQKFTNYLKRKQQEKMERRYHLNNITEDSNSMKQLDKQSYNQLKILEYFNKFHKEVDKQLRDLKEMVPSNPQTYVIEELNQQENINKQIEQKIPQKVIQQEQKYYPLFKQAKNSGQSIQNSYNQSQLILGQHQTHPQLQSQNSTQFQTQQNQNNNTQKENNQQRITTTQNQNSSEQQTETIKPILKQRQEIKEQLITFGRLQKFQYQKLIYPNQNEGNCVFCGLEANDQNLGPLLLIPSNNNQNQKYQLHEMCGLWSQNLVFFNGIEQCDPKNIDEEVDKSKKTKCFLCSRSGATICCAVCPQSFHFTCLMKSSQTGKLIENQFKFFCDKHKYKARLEMQNSSDEEQLPKKKKKLQQKLNTSKQMNKKSPKRGKSPASQQAILQQCLKNKDEDLKRQWINSDLIYFNLPQWASIEQKFSDLDQSLIQNKQYDPQNNIQSDIKQEQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QB64</accession>
    <name>A0A8S1QB64_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1530028</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QB64"/>
    <dbReference type="OMA" id="FVEICIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="162"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="418"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="526"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="529"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8112858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="66216" checksum="5CC7D34C0FB834EF" modified="2022-10-12" version="1">MNLSKSNSQKGFRILQKSSLSPSHKLSPEKSKLSFKTRKNSELLKLMVSPKLITNQTTQEGSFAKRETTLQNSQIMDVVLVIVHVGQQQFRFTFTRNITTTQMRAALLQKLKLKIVAFTTIDHNILIDYYLTLEERPIIWPELQLECQEPIQTGQHVSLKDFQILKCIGAGGFSKVYLVRSKINGHFYAMKLVDKEFIIKYKKAELLQNERDIMAFIYHPFTIQMLFSFESRNFIVFILEFCSGGELFYQLKTLKRMSEEQAQFYFVEICICMLYLHQMGIMYRDIKPENILLDLDGHIRISDFGLSKLTSPDEFAYSFCGSPEYMAPEMLLKRGHTIQVDHYCLGALLYELVTGLPPFYSKNTQKIYDSILNEQVTFPQFLSQEIKDLMSGLLAKEPNKRLGVRGGVREILQHKWFKKVNLTDILMKRVNPPIKPDIQKLNFETKNLQQGDLEVREKLVGKAGFKKDFKIFDEFYFDYRENVRIVDQQRLLKEHMKNVDSIQQQKPRSLDKIISEDSRQSKLQKRLEQQTSLRTSPQKGSTLMTNNFMQTGGLKTESSDLTSSKRRIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QD66</accession>
    <name>A0A8S1QD66_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T1550022</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QD66"/>
    <dbReference type="OMA" id="QSTWNDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000035">
        <property type="term" value="F:acyl binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863:SF28">
        <property type="entry name" value="ACYL CARRIER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 12" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Carrier" evidence="13">
        <location>
            <begin position="41"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8113363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="119" mass="13823" checksum="4D4D31E475D8CFDE" modified="2022-10-12" version="1">MNRRLMQMFSKQFGQIPKFYASNEQPIVLRNEKSGYYVNPEAVARRMIKVISLHDDVKNPSAITLQSTWNDIGLSDMAYVEVMVEVEREFEIEFPDADVECFRTVNDAVEYVARSFFAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDN7</accession>
    <name>A0A8S1QDN7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1560114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDN7"/>
    <dbReference type="OMA" id="SPAWDAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035734827" evidence="1">
        <location>
            <begin position="21"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="182" mass="20930" checksum="7429B9DE876B10D0" modified="2022-10-12" version="1">MRFILLIFFLEYSNFFGVKAGLCQENLFQRQKHILRGLKLDVASTFHGSFLSTARTNIFWRKDIDDNPLRFSLYYSYPTIVVGHIVMPLGTSAIVEFLQSYEINTIRFWMWEGGHRITNMQVFVIGVDGITETIVFDGLAQPAVVKAKFSSQIVSKIRFFNRGGNTVDSNMSIIKIQAYYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJ92</accession>
    <name>A0A8S1QJ92_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1610014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJ92"/>
    <dbReference type="OMA" id="ESAVYNM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="127" mass="14717" checksum="3D318E018F7BD2AF" modified="2022-10-12" version="1">MNILSEIKKQVEIEMKKIKKVPVSNKTITCQQKKELFDLVNQGILLKDAAKQLNLTYHEAKIVYNEQKRKSMNTQSETESAVYNMRTAGVSDLKVFPRHFLLQQSVNNTLVSVRRLFNILVLNPKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QK78</accession>
    <name>A0A8S1QK78_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoglycerate mutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1690014</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000178">
        <property type="protein sequence ID" value="CAD8116069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QK78"/>
    <dbReference type="OMA" id="AIFSMTH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045820">
        <property type="term" value="P:negative regulation of glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043456">
        <property type="term" value="P:regulation of pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051695">
        <property type="entry name" value="Phosphoglycerate_Mutase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517:SF1">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="2">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="26"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8116069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="24100" checksum="B7A99A29F98B503D" modified="2022-10-12" version="1">MDKFESLKLQIEKILDSDKTVLIFIRHGETEANFTKQLSGWHDVKLTEQGLNEGKQLSKAFQPLRDRFAGIFCSDLSRARLTAELALGSSDKIQQSMELRELNFGDHQNKVYSADNKSIYEILFTQQYQAPNGENWNDVQNRIMKYLRSQIHKKGVYLIFTHGGAIFSMTHQFGYTNTIKNCSCIGLELDINTLEIMNQLFYWEFPTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QLL8</accession>
    <name>A0A8S1QLL8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATP-dependent DNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1760003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000185">
        <property type="protein sequence ID" value="CAD8116613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1759" mass="205185" checksum="9A5D1F2AB578D23E" modified="2022-10-12" version="1">MMKVHQTLRKIMDAEPFYQISKYYNISSFQKEIEYAIFPNLYPKAVGGLITRAPNPKNMQLIIDIEQEEKKQALKYLNNIKEVREQNIQTKQTVTLEDLNNNTIYLNLELSDFTTKQKQGDYQLMPKKAVYNIKDEEALNNNLSFSYVYRWQYLSIYHYEKARKLNNESQSDDFDWPQIINLKLKINNDFLISSVLEKHVTSKLDEYLSKNQQQWDNEQKQYIQKLKNQSDKLKKLNKSKKEIESFIMEQEKLFIKNKIKNIIKQQKLDQIAQQLISSSSIIESGEEEMIKQGEQNVQKISRDLRSKSDSVISENQENNEYQDKDFQGRNNQVQNKKEEFMKFQNEQQNLSEDTKFKLNCSYTKLFQKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLFLMKHYKKGIKNKFKSQQNPDKEFNQYQYGLFGDYKALVTRIEFQNRGSLHFHLFIFINQNNNDSYDECYVKHLLGEFSLYNSKLEKDSQFLHKYDLFHHCSLERCITKDTQKCRLGFPKASNKGSYNVDNKIEIEQRRFIPEEKYTITFSRDTVKFFNSNCQINLCNDENILGYLTKYINKSPKQFKNVVGKESASKQQPQELIEKNSIMQHVKKYLDDKVTSLQEAYFAFKQTPIVESSFRVIKIKQQPLAVQISNKEDYQDYITVKNIEEMFEINQISNSWYRYINRPEELEYLNFYEYQMTTIFYGKKYKYLTKLKQQKSDQQQNNVNNIFLFDLNSNIIKIKEISSKQKVFIQTSHLHPSKEYQGINNVYFLQMLQLYWPHRKNSWIQTTHDNYDGYKNAFENLINTFDYPNEDSIKNQFRQKYCKFSEYPFKQYVEQYNNLQQFQIRILDNLFKFNFNLNKFNQQYKNYIQVDYIKSLINQIEYQLDQELEYQRVDEIRTVIQYLDPKKIIDELCKFQINELINEIKKDVQLFWDQMSQICKNIIINLGLICKEYGSSELFDLCPSKDSVQKYFPKKKSSQNEELFNFQSFDQQEILSQVQDDQNSQDPYLYRNAITILEYEDSKIIKYKDWATITFSINTQTQWENLKEQQRLCIILMISNIIQNYSREQEQKRINESTFLHGPGGVGKTFLIMFLIQFANDSGLNYNILCPTQQAVQNLRLICRSQNLDAINAQTIHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPH4</accession>
    <name>A0A8S1QPH4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1990011</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000208">
        <property type="protein sequence ID" value="CAD8117446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="WINHLEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="133"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="150"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="175"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="217"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="259"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="301"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="343"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="385"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8117446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="445" mass="48797" checksum="6DE9335802D8BF66" modified="2022-10-12" version="1" fragment="single">MFKQDNKKQNQMVILVISTQYVSLQMEIYQHLGVKISLSVYGMLRVRKKQPNQIVILMMSILSVSHQMEIHQHLVVVTAPSAFGMSKQDSKQPNQMVIVIMFCQSASLLMEIQGGDCSIRLWDVKTGQQKAKFEGHQQSVNSVCFFHDENTLASGSSDNSICLWDVKTGEQKAKLNVHTRYVNSVCFSPGNNTLASGSNDGSIRLWDVKTGQQKAKWDGYTIDILSVCFSPDGNTLASGSGDNSIRLWDVKTGQQKAKLDGHTNYVLSVFFSPDGNTLASGSADKSIGLWDVKTGQQKTQLDGHTNAITSVCFSPDGNTLASGSYDKSIRLWDVKTGQQTAKFDGHSNYVQSVCFSPDGNILASGSDDKSICLWDVKTRQQKTQLKNHSHNVKSVCFSPDGTKLASGSGDASICLWDVKTGQQKAQLDGHQYQVSSVCFSPDGXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QT74</accession>
    <name>A0A8S1QT74_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2640008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000273">
        <property type="protein sequence ID" value="CAD8118831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QT74"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="39" mass="4594" checksum="0A698014B59ACE03" modified="2022-10-12" version="1">MLINQQGKLYGKNNMKTGLCSQIQIRFYIYLCSEMKFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QVY8</accession>
    <name>A0A8S1QVY8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T2770002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000286">
        <property type="protein sequence ID" value="CAD8118994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QVY8"/>
    <dbReference type="OMA" id="FLYPQTV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12277">
        <property type="entry name" value="RRM3_MEI2_EAR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007201">
        <property type="entry name" value="Mei2-like_Rrm_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04059">
        <property type="entry name" value="RRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="35"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8118994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="15564" checksum="394C34E8B426DB2B" modified="2022-10-12" version="1">MIQDKLIFLNEEKYLQQQQASLEIAEENILKDDRTTLMLKNIPRSMKPNDLRNILNKEFRNLYDFFYLPLDNNNEGHLGYAFVNFINQDVVLKFYRTFNNQKWTNTEKQICQLKYAKLQGRRQYEFAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMN2</accession>
    <name>A0A8S1JMN2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMN2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8042915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="244" mass="28228" checksum="8BAF6730EA855E5D" modified="2022-10-12" version="1">MKKQLPKSTSQTAIPKPEKSQTIKTPERHGIPRNVNYYSHFLGEPYITEKDIEFVLQLRNIDHAEMTAKLARIPNQTFSRGDQKIASQKDKHLSRDVKYNGNSTPVLHLLKGRIGPTPHLSQAEFETGLRSYAKTDQSLLEKERNWTTVPKTQRKDVFPEFLPNYKEEIQKKKSMSISGGKLAKLTYNAFDSDSNYPPYQMKFHEKNLQNVKHMFIPAIKMSTVQWQDGLRPLDTLKKESKTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMP8</accession>
    <name>A0A8S1JMP8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0050196</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMP8"/>
    <dbReference type="OMA" id="EWFKENI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000447">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="71"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="359"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="397"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8043543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="58911" checksum="164F87B0D7653CC0" modified="2022-10-12" version="1">MGQCSSQKMKQQMKAKIQEAKQKQSQTTTPNRQTTQLNGMRSQSRHNTHSAFQSHNDLSQPGYSLKNINYFLVYRNTNFSSYYSLLKQEDKDFPFTTIQHNNTGIVRIIKSYKSSDSKYIAQLLKYQLHHPNLIQQFEIYEEQQKYHVAEENNNKIVVLNSKIYNDEKEIAFVFNQIVEVIEYLHHQKLTHGHLTLECFALFGDKYLKLYDLYHLFMKKKPTLEESHYLPPEYFENQEYSEERDIWSLGIILYNLLYHISPFQSNIPSLLKLEILASKISYEKTISDEAISLLQQILEKNPKSRISIRDITKHIWFKKQQVFLLDEQIKETLLRLKQSKKLNILQVYLLKFIINNYPPDKLREIYSVFRSLDLDNDGYFSISELITSYTEYVEDTENTKIICMNIFQKVDMDKDKKITFQEFILYAFDRKELIEEEVIDISFKLLKNKKNFITAETLATQYTLDKEFFTEIMIELLKKDYITFQNFKDLMNKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN89</accession>
    <name>A0A8S1JN89_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="11">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0040277</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="11">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AdoMet synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="11"/>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JN89"/>
    <dbReference type="OMA" id="ASYMARY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000004">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000011">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="13">
        <location>
            <begin position="23"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="14">
        <location>
            <begin position="134"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="15">
        <location>
            <begin position="257"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8043057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="419" mass="46669" checksum="6DE0571EF384CC20" modified="2022-10-12" version="1">MQQVDNNQESPHKKKFVLPKGHFLFTSESVSAGHPDKLCDFISDSVLDACLEQDPNSKVACESACKNSLVMVFGEITTHAQVPYETIVREAIKSVGYDNINKGLDYRNASVIVSLDQQSQEINQAVVGSKHEDEIGAGDQGLMIGYASDETPELMPLTHHLCNKLISRLQECRVNEICPWMRPDAKVQVTVEYKREDSSFYPVRIHNVLISQQHDEKITHSEIEAELHKHVLKYVLPSKYVDEQTQYHLNPSKAFTVGGPYGDAGLTGRKIIVDTYGGWGGHGGGAFSGKDPTKVDRSAAYAARWVAKSLVASKLCKRVMIQVAYGIGISEPLSICVNSYGTQAEGLDDDDLSEIVQNHFDLRPGVIIRELKLRRPIYAKTASGGHFGRNEPEFTWEHPKIINLSAWKAHKAEQQQQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNR8</accession>
    <name>A0A8S1JNR8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNR8"/>
    <dbReference type="OMA" id="ILRMNYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="95" mass="11482" checksum="8D0C079FC368FD19" modified="2022-10-12" version="1">MDWTSFKSKFAYQIYFSYTLPDTHEQMIETLCKDQFETPLHYKQSNIRFQGASLKKLLKKDSNIIHDLQNVHDFGDQILRMNYQEIKKQVPYFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQF1</accession>
    <name>A0A8S1JQF1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0060227</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQF1"/>
    <dbReference type="OMA" id="SLCQIYN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="489" mass="58055" checksum="3D7EA67965CC6DA1" modified="2022-10-12" version="1">MLQSKCDELYPQYIPQSFQDKLLQHHFAPNLTFQEVIQNLMSEDFIKIHNGLISYKNQLMDVIQIENDIEDQQITLLITILQQILQAQMPPCIQFYAINNLCQLLIRTNIQHQLQIKQISFESLCQIYNRDSPNPFQFQILNIFSIFCQLDKTIAYQIFKLCHIQQKLDQIKQLIDVIHYLQALQIFCKQMPQCYKNMTLNFIKKQFIFIQKPSIRACKIAIQIINDLVSIENPELDQQLLNIWDEIIIIWLNDNLNELEGSMIQIISKFIKKANFPKEKIKNKVFLDRLFHNLQGDQIFNLIDSLQIYQFLIKQFQICIADDEFFQKLMQLCKNSLNPNITSIILQIIIQMLKSKQLSKEYLSKLQQFNIIRLLPEYLHEAMILIEKINYSTQLSGFLKQKQINELTLVNCIMKIVTLILKQNILIDKFKTEIAQFERMFQSEKFQTFMHNISVMKIQEKFTLIKGLLQSIISYEDYESSDNKKIRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZ17</accession>
    <name>A0A8S1JZ17_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZ17"/>
    <dbReference type="OMA" id="KQNCQSK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="515"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="632" mass="74712" checksum="DCAD3F972263052A" modified="2022-10-12" version="1">MSVQISSSESDEKSFIQTEQERILKKSNIFKQKEQCNNSCAKHPNKKAKYYVQQDISKLFCSKCALNLALKGLKIEETQEKQEKQPEIYRQQRIQSFQEQLREVLKQCSIKFKKLNNLQINSSKQLQDQKDNCNLFFEFVINIAKQLKLTYLSKIENDHINQLNQVTDNISLIQQIDTQLKQYEIDITTNHENIVKNMEMKPFEDIMSRYDKKVSQVKQQIQEFNQEYIQKSIQFEYNQILTTMNKMCYNLLLNTEDSSEYVNYQIQNQNTPKIIHKATNSPINMKVFELLKGDDIYQSTCSNPVKDHIQSPGKSSTQILQQQQKQKENAFSNQIESNTYTNTPENWQFKVFQDRKNHQVTPNDRDSVKHNISVADQINNNQFELNDKCIIQQSEKQNTTIECQKQQENSNQLLNNNINQLYIQRQTKEIDRNSFEDRQLTPKHQKHFTNAIPQTFKLITNHINQKSQYQYPLVNNNLLEQKNQTEFSKKNYDSQPQQQSLTPLHQELYSRINNQQNHKQDNNMNRRTNSKQPSLDQIEGKRQFILANMNIQQYTSQANQNTTNEDTLKDRIIKELCSHPGESIYNQVLKQNCQSKQKNQKQTSKENIEQSTTLRMKNISGYQCIKKQSYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3T9</accession>
    <name>A0A8S1K3T9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mini antigen</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3T9"/>
    <dbReference type="OMA" id="NICTWNP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035735666" description="Mini antigen" evidence="1">
        <location>
            <begin position="19"/>
            <end position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="785" mass="85564" checksum="3508F1A40D3C8A31" modified="2022-10-12" version="1">MKISIMLLLVAISAIGNAYFVPNVKCACQQILIETECNNWNCQWTIVTPATSTTPATGYCQQATCGQLNQQQCLNESERCYYQEPGPNPTTSQVCYEFSKCEDIVLSNGATCQKTNPKCVASPQQLRPKFQCISAGLTCSGLSMEQCSPSQSQTLAGEGLCYWNGNSCKVVSQCSELTTITQCQMKAFQNACQWSGTACIALTCASFQQSETCTFYQQATLSSSPIQICNWTGTNCIPATSQMLQSSNCGQNTNNHYRWVPMNASSGICAQCNPYKYVFRNQCECKQLLQANECTSSKSCTWTPPAGTTPGYCAPTACTSFTDQQTCAQVQGCYWNINAQSPGCQPFTTCSSITGQNAQICYSQSIYCPGSNGTNCQSQQQLNSCATIVTPMQPNVCFNSIGKDGYCTYQNNQCASVTQCSILTTLQLCNQFSNSCIWKAGSQSSCQSKTCSDYLTESSCTYIQNNINYNIINICTWNPDTKKCSTFDNSFNYDINSCFNSTGRTHHWSEPSNAQKGFCLPCHYSSVLNVKSNCSCNDLTNYTDCALSSPKCYWNAANSTCQNQNCPQLTSQGQCVLSKYCYWSNSHGCQPLGSPSTTCSTLTANTQQECLSQSILCAGINSNGKCQSSPNSCGDFTTFYQCFGSVGTDGICQWNQTSLKCQGISNCGLINTQAQCELVLNTCYWGNSGNGTSTCMSYNCTTLYTQTQSCNFYLSTPNQNYEVKYCQLNGQTCAEANNVSSLTSSNCYASSGGTARWDADKGNGTCVQCYAKIISVLFIILSYMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4S7</accession>
    <name>A0A8S1K4S7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4S7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="125" mass="14749" checksum="B19F34700ED9B2AA" modified="2022-10-12" version="1">MQQKEYSKKRNKYSDSEGYFQKTSNGIGYKNNYNPYLDESVENYNNKYSKSDYELNWYNGKKDREPNGQLQVVLNGNIVMILKQQIKTNQKRQNRKYSDQETYAMSNSISGPKFSDIPCPNFLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6E6</accession>
    <name>A0A8S1K6E6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0120168</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6E6"/>
    <dbReference type="OMA" id="SQTRCVG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000006">
        <property type="entry name" value="cAMP-dependent protein kinase type I-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000472">
        <property type="entry name" value="cAMP-dependent protein kinase type II-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0114">cAMP</keyword>
    <keyword evidence="3" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="123"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="247"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8047856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="377" mass="43993" checksum="D1A26A081A6A0E31" modified="2022-10-12" version="1">MNQSAEKLRQYAQENVVFILQKLMEDILAQQPKNVIDWSIAWLDKKGRDIVKQRQKQNIHSSSSDDEVIELPKRPAAARKQRASISAEAYGQYNRKESFQPRVIVKTQQQKEKIGKRLEQSFMFASLDQREKDIVIDAMEERSYNAEDWVIKQGDNGDNLYVVDQGELNCFKRFIKDGENKFLKVYYPGESFGELALLYNAPRAASIQCKTDSILFALDRQTFNHIVKDAAMKKREKYVNVLKQIELLSMMDPYERSHVADAIRSANFQKGDFVIREGEQGDIFYMIEEGELIATKTLIQGQEPVKVFQYKEGDYFGELALLKDIPRQANVVAQTEVKLIYLDRHSFKRMLGPLEDILRRNTEKYQKYEQYWINQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9R4</accession>
    <name>A0A8S1K9R4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9R4"/>
    <dbReference type="OMA" id="WNHININ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="133"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="408" mass="49049" checksum="43114E83A24A9978" modified="2022-10-12" version="1">MNFLSIFTSSTKQQQKQQKIKIYKDEQCQNYFQNLNIEDNTQCQKILDQIDIQNQLGDDVRKVLLLFTCQKDKKCISQRIINKQEQIAKILQNAPQNLELYIIYQEIKLSKLLETQSYHQKIWNHININSKDHSFRQGTLLKKGKTGQFRERLFKLQKDTLIYEKQEKHEKNKTKQSCISLDSMQIEKQPDKNLTFLIKCDQKIYQIKAQSRTDFDGWLYSLHNQIMKWKEVKLLLDYDKKIEQCAYQKVEKLKSLPSQIKSIKFIAQNNTYITKQFLKYLENNKSQLDNKLFVFLQLTLESETLQQGSKYYKQMLEKKDSLYQEIQEMMKDDEHFSKFEKIQSTQFKQEITIYFDKQIVPDFLLQIQQSQIIQDFSIHQFKQFYKFPLFDLSGFYQDPTDIVHPNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDH5</accession>
    <name>A0A8S1KDH5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDH5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="223" mass="25968" checksum="ED8F1E0F7047EEE5" modified="2022-10-12" version="1">MNLDLSQQLQNYRQEIHNKFKSKQIMAQTIFKLASQQNKKDNVNLATLKQDLSRQKTKQRTSVSNFQGSPLKNVVRQNQSPNVQIRNEFNSSKFIIPIRSMSQFKQSPQQSKFQIPTPRDVKQNQSAVKLYSSKYQKVLPSEQGNYYGQLQAKLDGLALLIGSEEDYHQNAKDVENVIEETISNYQSSNKQNKFDKLKSQQQLLEQDLKQLTFKISRFNVYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHX6</accession>
    <name>A0A8S1KHX6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cep192-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHX6"/>
    <dbReference type="OMA" id="CMQKQQL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054089">
        <property type="entry name" value="Cep192-like_D3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22067">
        <property type="entry name" value="Cep192_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cep192-like" evidence="1">
        <location>
            <begin position="220"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="311" mass="36106" checksum="C8ABCA10920ADFB0" modified="2022-10-12" version="1">MNQLTESQNILNEQQQLHFEQHEEMHKQKLLIQPLSPSNKENRQEQFGLQKQQFLQKSNNDIYRQLKNEYSELKNIINVNKQSVNFGLVLPGNICEDDIQFQNISNETIIIGIQVVCNNTEFDDLDEYVYSARKLNGYDYKDRFMLACPAQKQFNMKVALKVPNIKDQKGLFGTIIITAINSNQQKIQGQINTNIQSQVLLPLIECSKRLMNNFYKVPIIQMALKLGKKLDCKIQFRNNSRIGLPLELEILNKNNCEIIVNPQSLSVQSNSQFLVTMQVKNQQVQLIKNVLIIKVKNSNVHFSYPFIIQVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIK8</accession>
    <name>A0A8S1KIK8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0220340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KIK8"/>
    <dbReference type="OMA" id="SIKNQMH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="129" mass="15400" checksum="93892BDBE741EE9C" modified="2022-10-12" version="1">MFKINCYEDGLNVLIASIKNQMHQSIRKNKFQIQDIRKNDDIEITSNLLSIRSSIRNCIGNSQTLSRYSRLKETKRRYSLLPLKNNRFRSPLKEKQKLKTRDLIKEKLMNQNFEIRAWTIPNTNQTDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSE5</accession>
    <name>A0A8S1KSE5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSE5"/>
    <dbReference type="OMA" id="ICAFHYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="381" mass="45780" checksum="7CE8BFFA53CF5201" modified="2022-10-12" version="1">MEPPKYAQKVEENQEAMNIVLGIKIKQLNQNELANTILYKQFSFVSNHLIKVITRDEQSYRFKIHFGLCKMMEFNELELVYWHLLNLQFDYGKKLQPNYLQEIKICAFHYFKDFLKIQAEFIPDIALYKRELLFLTLSAFYTIKLMRNIQKFEKIGEFIKEDKFCELFKRIEPAFINVSQLLTLQVVNQKYIELTKYELQLDKQETNYNFIVEQILEMAPAYQKDEDLPISKKVKRQKKLMSKKIQINKKQEQESQQKIEAQQSQQFQTSQYQNQQLSQNEQQQINQPMGSIQFSRQFNQNLQSQGHQQIQQDNFFLNERCSFQFQDRQNSFGMQLRSNRYFNSQDSLGQIYSNQFNSTPPKETYNQRNYISSLWTNKNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVI9</accession>
    <name>A0A8S1KVI9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">cyclin-dependent kinase</fullName>
            <ecNumber evidence="1">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0270202</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVI9"/>
    <dbReference type="OMA" id="PARRWNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07833">
        <property type="entry name" value="STKc_CDKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000049">
        <property type="entry name" value="cyclin-dependent kinase-like 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001072">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="4"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8058411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="454" mass="53004" checksum="8913CB7D75A3410D" modified="2022-10-12" version="1">MNKYEVKGVVGEGAYGIVLKCENKENNQIVAIKKFKETEDNEIVKKSIQREVKMLRQLKHPNIIDLFEAFKRKGRIYLVFEYVEKNLLEVLQASPNGLDQGYLKKIAFQLLKAMEFIHYHDIVHRDIKPENILIDNENNLKLIDFGFARSLNIPDTLTDYVATRWYRSPELLLNYQTYGKGVDLWAIGCLLCELTDGEPMFPGENETDQLYLIQKTLGPLTQEQMEIFQKNPRFLGMKFPEIGKPETIERRYLGKLPQKAIGLMKGLLRMDPKERFTCLDALKHPYFADYPEAQEYLKQVENRQNKVQNDVNQKRDSVLSQQRQQIQIGHNNQIRTKSIKPVPNFLSNHVLMQSTAYNYKISDQHTAERELPNMKKTTSVDKLPQTQYNGFNIGNGFSFAPSKNQQLKATQPLKQSQPQIKIQNLNIIYNTNTYNYQQQQQPQQQQRKIIKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVR2</accession>
    <name>A0A8S1KVR2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0250054</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVR2"/>
    <dbReference type="OMA" id="NPYYSAW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="11">
        <location>
            <begin position="17"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="190"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8056786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="411" mass="48019" checksum="68A72AFC93514408" modified="2022-10-12" version="1">MYKSFKSYPSFAAFQIEQSKSNRLLYVYHNSVYDLTDFIDKHPGGRGPLNTYKGRDLENIFFNPAIHKHSLSALQTLERYKCGIIENKPIQQQCQQQQSMLGSKSTNLSQSIVTNKSQKNTPQKIDCEKKLQQKSQSVKPKAQQQEDRLNQSMSKLNQSMTSSVKSQFLLIPTQQEILMIQKQKQQKLIKQWKQSEIEELKRQQAEVEKLRREKNDDRKTQSEILVSNPKKSTNPYYSAWLEKQQQQELQKKAESKNSNILPAYLTQTNDQQLNNQGDIKFQQFSFDVEKKRQYDSKLSQSVIVEKTQKKVNPYYEEWKRRQSLKSQESQKELVEQDHNQLQKQQSIILEENESQISGQPSSINNSFQRSYSMSQRTIVQENPQLSVPVFAPKFISPIKAKFVNSSSMKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZ13</accession>
    <name>A0A8S1KZ13_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270341</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZ13"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="146" mass="18115" checksum="E6BB9BF9239BD1C9" modified="2022-10-12" version="1">MQISILVNYRQYSFYRTLDDILMIFYLKLTIQNQKEKHYRQLYKFKWRGTLNSKIKLKINRNSYVENVGYHECFAIMFRLERLRLYRNNINLINIWIVFFDRIMSGVPFVETIQKKTEYDNEFFETNRNVLNMYFEQMRSAKQLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZC4</accession>
    <name>A0A8S1KZC4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZC4"/>
    <dbReference type="OMA" id="IMINKAE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="60461" checksum="058DB366B67C74EE" modified="2022-10-12" version="1">MSDQFDQSQEGIVQNEVPENRDQELFDQVASRQNNEKKQNFQIEEEDEQDQNQPVGAIQEVVKPEEIKKNVSQISSPLQPIMINKAETKRIVHQAALERKTEAFEQTKSFELHRSSQIFQHRQLVDSVWARLQKVVASSDESMKFLVSFMKQRVEQEEQQLKYSQNAQLSKLFKDFDGKIMQCNYPEFSKAVRTIDSAMERQTDQIKIFIVWIQNQTRERLEQEMSNFTNQNRDLINSYNRFKKTLTDLDKEVTKYFNKYDKMYQNMTIKGKKPQKDFLRAELKYKQAASRVVIYQREFGTWLLNSWNEIKLLEVQRLDLVTHTLMEFQNQIMNVYGKIPQNDSIIAALNGVKVQQEVQTLYSHDFVLTKQELEFIQTEQQQELVKYFQSFQVIDKYAELSHPLILKQFSAQRDVGTIGKTWIETGIIITVDHYFLTFDGKPTRFSRPENKYPLDGMKMTTRSPLELEIQFIIPGLVMDSKKKLLIQFKKSDELEEMMYFLDTVGQNKFALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2G3</accession>
    <name>A0A8S1L2G3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0290088</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2G3"/>
    <dbReference type="OMA" id="IDVVCDM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000008">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF149">
        <property type="entry name" value="GH01077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="10">
        <location>
            <begin position="52"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="11">
        <location>
            <begin position="197"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8059632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="467" mass="54447" checksum="B0B466205F3C995C" modified="2022-10-12" version="1">MIIRKVLQKGFSTLYNLKQFRHQEIPQNVSVDREFGDTILSNGIRVCTEFWPSDLAHITIYIKCGSRNETEATSGTAHFLEHLHFKGTGKRSRDRLECDVENFGGQLNAYTSRENTSYTINAQKNKAENAVEILGDMLTNSIYAKSDVERERHTIYRELFETRKMQFETQIEISHRSAYKNHQMSLPILGKIQNMYSITRDMISEYHQNNYYGENLIICGVGNIQQEQLCEYVTKHFSKVHKKKQQLKKEVPVNFQNEVFLMQSELTEDINVGLFYQGPEWTDPHYYHFLILQRLLGDKPSNFLEAAIFEQSTLNSFQKLLLNYPEITTQKAVYTPYKDTALFGNYFVVNPNQLDSCIEVSKKIFEEYGEKVSSEELQRSKRRLFIELCQHETGNDISQAIANQLLYFDRRVYRQEIAQNLANVTELDIQNCVKNWILGKQPSLTIWGNIDPVLKKYKFDASQIGTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2M9</accession>
    <name>A0A8S1L2M9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0300133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2M9"/>
    <dbReference type="OMA" id="FEFEFHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039273">
        <property type="entry name" value="TEPSIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514:SF0">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="489" mass="57078" checksum="F0B34CD31D6BECB0" modified="2022-10-12" version="1">MSSILNKFRKQPQNDVPPGIKIENTLFGQQQGTYQPPIIPSVQLPQTTQPRQKGRPGGGWDDEPEPQQQQQQALSNKPTNTINLINDTPPKQNENKTNIDILLPPQSFNNIQLSRVTDYKKGQLLTYEELEIGGFEFEFHIVEEVIKLGGIKLKQSDSVLKEFARACQTLQEQLIGSILLNKLYGEENWKVQIRCIYAIQFISQQYQNYREFFQINQNYLKIDTDQQILQSAIDQTLLEINGQQIQKQKEIQLEFREPPKPQQNIVQQQTNLFEIGNNEGQQKQQQQQKLDLKSLKIKQPEVKQQTQKQQNTQIDLLEAFNQISLSNYDQNQQQQQQQQQQQQQGISFDEILNTQTLAQTVQQPQQKKNAFGFLKKDQQQQPQQQQPQQQKQEQVQLNSLLLQTPQQLYQQPALNQQQTSNIMYGQPSNLYNLQQQTQLGQPNYSQAFAQFNIQQQQYNLVQQTQFTQEQPQQIQQPTHKENAFDFLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9Q6</accession>
    <name>A0A8S1L9Q6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0340120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9Q6"/>
    <dbReference type="OMA" id="HQAGKHS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="257" mass="30317" checksum="754121F08EFA1891" modified="2022-10-12" version="1">MKRSSNKLQKQNASQPQTENRGKPSQLKFLQLNAPGIWTNKHKSLYIIKYLTDNGRHNFLHQAGKHSNKDADHENQQNNQDTKIYFEQAQEIAEKLFNIQCSSKRCIVFYFRYINEQKLTYDFSEYIQNKDFVDILSSIQNLLTENFIQDLIIILSSQNNINSIADMLLENKGAPSNYSHIIKNLFIQKKIDTMIEQKTITSDIETIRNEFEDFLKDPQTKYLVRMFICDILNIFYTQTIDQSNQDEQFQMAEVNDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LA35</accession>
    <name>A0A8S1LA35_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0360080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LA35"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8064347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="36372" checksum="62C943C8E58B87EC" modified="2022-10-12" version="1">MGQECQKCQMRPEVQHQSELNKKEEISSDSDSDPEYEEEDEKQPQKPPSMDNKSNSSTFFKEQYISQNSKHEQIESLQNQIKFEDGGIYTGMIINNKANGFGRLVMPNGDIYEGSFINNYLEGEGKCIYARGPIYTGQFKQGKSNGIGKEVWPDGSVYEGEFQNGKKNGKGIYKWSQQSTYNGEWLDNMINGIGKYEWPDGRVIYQLFRTQSYYGYWVRNQMHGRGFYKWIDGKYYDGEYEADKKSGFGIFNWPDGKQYQGYWLDGKQHGKGLMINKEGKKKFGEWQNGKLISCSDESNLQIIPDGWFLNTFNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDD4</accession>
    <name>A0A8S1LDD4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0380263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8066088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDD4"/>
    <dbReference type="OMA" id="NLFICYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="27"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="24642" checksum="75E027C24BA632A7" modified="2022-10-12" version="1">MNNNQFQHPQFTNVNMPLTNFKQQQDQFILKAEIQQLRQKLEEQEKKFQVVQFQKEDLEKKVANLEQQLFQRNQNNSNAKNIIEDQNLKDKYIQIQLHNQDLQKQIDQLNEKIEDKKRIEIQSIPIISKNIQEETVRLSWFEKIDNLQISFQRKNRIVEFQGYGEVQNKGFIFQCQCELSRIPVVQTQPQIVQKEHELFLKFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDH4</accession>
    <name>A0A8S1LDH4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0380288</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8066138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDH4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8066138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="36" mass="4329" checksum="754254CF2F3F57E5" modified="2022-10-12" version="1">MKSLSASKYNHNNTSFTTPDKKLNKIKYTVYYEVKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LEF2</accession>
    <name>A0A8S1LEF2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LEF2"/>
    <dbReference type="OMA" id="SSEFHYW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="387" mass="43639" checksum="7E42223A4231BCED" modified="2022-10-12" version="1">MLFLFIIVSLSYAATLIKPVTIINPYDQLCSGAGECCACSKTGYCSSNQTCTCAASQGSQMSGQTCLLNSTENSAVEKQLSNGADESSIASFSVIQVYDLLNRNSTLTYKQLFAIYELGLSIAKSSVQQSEINWFLKSIEFITKKLKAIQTLEVSALQTFQIQIIDLIRSLMFKYIDLDSKKNPIFFEQGYFSAIIAKWKDTINYSNMKLNSKSIQSAQCEYKYKSVLIRYKLVLAVSNDMIDAFTNSTFTYNSTVTYCQFMDKYGEIYYPPSSEFHYWHYVPNFDYEPLLEMSDVIGLGCFQIKLSNSVYGLAYNNQDCQYKEITSSRPVPYCQCNEPGITFVQVLWNDEKIAQLALTAGTSNSTNTTDSYGKLLIIIYLAIQMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFI6</accession>
    <name>A0A8S1LFI6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0370163</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFI6"/>
    <dbReference type="OMA" id="IRMRYSK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00124">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF706">
        <property type="entry name" value="DILUTE CLASS UNCONVENTIONAL MYOSIN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0518">Myosin</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="5">
        <location>
            <begin position="62"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="3">
        <location>
            <begin position="651"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="729"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="944"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="176"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8065231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1274" mass="149461" checksum="4653AB9933DA36F0" modified="2022-10-12" version="1">MNLGDDEIWVNHTKFVCLIAKQDNEQNKLMITKQGINSIDKEIREEYVDEKSNIKVDPLAKKGVSDLLLLNELNEFNCLHGIRMRYSKKLIYTQIGAPILVAINPYQYIPEFYDEKMVTFFKKEMGRMHQISKDILQQKLRDIDPHLFKVAQMSLDQLFEESTKTNLRICSMIISGESGSGKTESTKIILKYLAAESLGQKSKTPSIEQQVFASNPLLEAFGNAKTARNDNSSRFGKFIHLYFNPVTKRISNAKIDNYLLEKSRVVKLSSVERNYHIFYQLIAAQIPELKLGQAEQYHYLKQGDLKYNKKELEEFKETSDCMDEMNFTPQEKQYIFQALAGILHLGNISIQGDANKSFIIEDFSLKTSCQLLGLDSEDLIRIICKVQTVMGKEVIMKENNIEAAGSARDTLVKHIYEKLFNWLIERVNVQLQACGQVQVQQNTSQYMIGILDIFGFEIFTDEQGIQTNSFEQLNINFTNEKLQQHFNSHMLETEQSEYNMERIQWNQIKFPDNKQIIDVIENSTLSIFKLLIDQTIAPKGNDREFSNSFKKLPRYCVFNVQDLLAQDSDIKVDKKHKTAKFDWFGIKHFAGYVIYNVSGFIDKNKDTINQEVHKVLPTSKNPILREIWKSHQITNTNIKQNNVITRFQNSLQELLGILNKSLPKYIRCIKPNNTKSAFEFDAQEVRRQMRCAGLLEAIQIRKAGYEIRLYHNDFMKKYRFLIKGKPTNINQMIRLLNENQIVKEKLQKELEAKNLQIGTTKIFMKEGLRDFLDQQLEEFRIQKKIKIQKFVKKYIYKKTIFLKLKMLLQRHYKLQRSCKWYLYKVVFLVKRIKKRKILNSFIQTIQKFEKKIVEFFAFQKLHDYATILIQRDAEIQRQQQELEKELQIESSGEINKSVNISQKLVEDDSSISQSQPIQSFNIKTFTKLDPVSNSASHYMLKKALDEATLKLKLEQDKRRDLEDQVEKLQNNLKQFESSGRETLNPSAFGQLEDIFTLQKLTEEKKMLEVQVEQLQFEKKRIGKSSFDQIQNLSLQLAHAQHTNEVLTEDYVNKFNANQEIEEKLKDEWNQERRQLARELEDCKKALSNQNKIAQKDIQRIKMEYDREKLRVAELESEVLKAKQISELQLKDSQVEIQLKDQTISQLNNQIKEYNTRSLIQSQVISQINDTPEVIQELNEKIITLSATLKKREFEQSRNKKLIHTMNHLVKLKIIEVNALHKHFNIVHEQQKQEIYQPIAYFMEKEKILIKQFEELVKQMNQESTGHAENQQKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFJ7</accession>
    <name>A0A8S1LFJ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFJ7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="132"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="471"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="503" mass="59763" checksum="AF9BB8EB865BB7FD" modified="2022-10-12" version="1">MLLQRHYKLQRSCKWYLYKVVFLVKRIKKRKILNSLIQTIQKFEKKIVEFFAFQKLHDYATILIQRDAEIQRQQQELEKELQIESSGEINKSVNISQKLVEDDLSISQSQPIQSFNIKTFTKLDPVSNSASHDMFKKALDELKQKLKLEQDKRRDLEDQVEKLQNDLKQFESSGRETLNPSAFGQLEDIFTLQKLTEEKTMLEVQVEQLQFEKKRIEKSSFDQIQNLSLQLAHAQQCIKELEHNNEALTQDYLNEVKANEEIEEKLKDEWNQERRQLARELEDCKKALSNQNKIAQKDIQRIKMEYDREKLRVAELESEVLKAKQISELYKSQLEQNDSNIKDHKGNEHELQLKDLQIRDLQVEIQKRDQTISQLNNQIKQNNSKSLTQSQVISQINDTPEVIQELNEKIITLSATLKKREFEQSRNKKLIHTMNHLVKLKIIEVNALHKHFNIVHEQQKQEIYQPIAQIMEKEKILMKQFEELVKQMNQEQTEHAENQQKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHJ7</accession>
    <name>A0A8S1LHJ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0400210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHJ7"/>
    <dbReference type="OMA" id="KSVQHKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="252" mass="29569" checksum="DB7B885D96497D04" modified="2022-10-12" version="1">MQINGRLQFDLKLDDKDYKISGDIFMKENISTDVEKEAETIANRIIEKVRQKSLNKSINKSVQHKYESQSATKFSQTEHFKSIEQSNSRQETLKNDYKQPYLSEYQTEFRSSCKLNNIDQQQQIDICELITPQKHKQRNTKSQQCIKKDQPIHKQSTTIQLAESQYIIENNTNSNTKDGKQIIQLIDNAHNLIRKNSQILNKVQLQSPLQSVINTKYLNTQNNKKKEDIVTQLLGSLRNAKKIKDEYQKRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKY6</accession>
    <name>A0A8S1LKY6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0430030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8068848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="35"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8068848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="21076" checksum="5AA03001309013CE" modified="2022-10-12" version="1">MNYKTKVYLIKLFLMTGKLKITNLVKQKPNQLKVLQVYKNQGKQLMDLNKFDEAIQIYINILELDSKNIAALFGYGDTLVKLQQFQLALEQYRKIKKFEKDLIVAFLKIGLTLGQMGNIKQALKYFIKTQNEIMEDHKLEWTRNKEIEIKELLKQALQYFSNQNLTNEELLHLFQXKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LLE8</accession>
    <name>A0A8S1LLE8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0420219</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LLE8"/>
    <dbReference type="OMA" id="AYQVKND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1087"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="160"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="264"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="382"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="775"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="856"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1096"/>
            <end position="1111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8068557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1111" mass="133964" checksum="91F8DB3BB13428E5" modified="2022-10-12" version="1">MNNRWSISNQASLQQSRYKQEIDNSNVPNNQPAYQVKNDEQARKLGQKDSPILSENRQRMKSSYEVEKSSISQKYQKTETSYSPIIKSTYQRDISPQFQKSENYKLIDSYEIRLMKLSQENGQLQSQLFSDQQKYNQIIIDFELQTKCLEQKLHTEIMNIQQHKLLIQNKNEEINAQTQELLKLEQKYFDLESKMFQQQKDMGILKQSNDAKTIEIDSLRQYIKELQAKNARDYEELKLSTDSSIRINYELQIKEIQVQHGNNIARLNDLLQQEQQKYNRLLNQFEQQMNELNYQNIKLNDFKIEKDKLFEENTRLQRLLDEIKVQQQELLRESDSQRERQMQQDKNHQTTLNELKSHYEYMKKSQVEREVKEVTIKFQAERQSYESQIRSLNQRLLEFEGRLNQKNEENRQLQQKQQAIESEMRVFVQDHEHYKRIKENELQELQNELQKQRQLQNDTQLKNQNQLNEIQRLNQIINDFKTEIDLVKSQSQEIQKQIQIEYDNELKNQKSKQQYEISQLQDQINDLKNEHQKYDQIIKQKDETIHQQKLNLSQKQGEIDGLILKLENLEKLKEQEINQYQNEIDNLQREQNLSQTKLTNEKNLLEQQIKQLKQKLNELETQQIQQEFNNEQGRQELEQKLQQKEFQLQQLQNEKNSINSQLSVYKSKIEQLDQIIQELREQNQQISQEIEDQKQQNESDRAQFVKKELGLENKIQQLKQQINQREQELQQYLNDLDSTNNRVRQQELITQQSQDEFRRREHQYVQEITNLKQLMDTTQQRQSEYQSMRETLQEQVQSLQEKSLNKEKEVEEQQQKYEDLRQQFNQYVEETNLKLEYYQRIEYERKKSDLKYQAELNAAQDKIRVLEIELETLQLRQNQLLKERRELENQLDEKSKEIESLKNQLNKDYISNEEYTLLQKKYNEQTDELHLLEQKQIKFGTEKKQLERQIDKLNQDIELKDQELQQTDNLMKKRRSEQDELNRKIDSLTRDNAKVSHDLLDVQAQLSTKNDQINALKRENEDLQIQLASVKKQYEKTYEQLNKKNQELLEKFNDAEIKKSYSSGENTATKVLTATTIIKTSQVQLNTPLIPEEQESQQGTERSDQQFKTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LM43</accession>
    <name>A0A8S1LM43_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin fusion degradaton protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0400247</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LM43"/>
    <dbReference type="OMA" id="YKEHSEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004854">
        <property type="entry name" value="Ufd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055417">
        <property type="entry name" value="UFD1_N1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055418">
        <property type="entry name" value="UFD1_N2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555">
        <property type="entry name" value="UBIQUITIN FUSION DEGRADATON PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555:SF13">
        <property type="entry name" value="UBIQUITIN RECOGNITION FACTOR IN ER-ASSOCIATED DEGRADATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03152">
        <property type="entry name" value="UFD1_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24842">
        <property type="entry name" value="UFD1_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 1" evidence="2">
        <location>
            <begin position="10"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 2" evidence="3">
        <location>
            <begin position="106"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8067362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="32049" checksum="A00F6498C4040ABA" modified="2022-10-12" version="1">MHRSYRGYHDNLEVFGASTFSKRNLNQGNKILLPASALEQLIRSKQQGPMIFRLQSTQDNNKYTYVGVLEFTAEEGTCVIPDWMLESMGFFDGCQIIISHEKRLEQGKLIRIQPHETAFIDLPDPRAILENHLRNFICLTEGETISINFHNINYSIDIVKVEPSNILKAVCINEADIEIDFLKPLDFNDAPPNLVKKSSSLVQQEELQAQKQQTVFTGTGVRIDGKPLNTQMRKPSEDVKTVEPYDPRKQKLKNGLKQTQEATQFVGSSIKLGNTGTQNKKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LMK9</accession>
    <name>A0A8S1LMK9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0400286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LMK9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="416"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="510"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="625" mass="74628" checksum="C133903E19C7B64B" modified="2022-10-12" version="1">MNTITQQLEQYKPEIQQNICEIHKSDIVAIDLVISEKCQFQYLCYHCLVAKINDNNISTIEQTKERIQSYKTKKQENKSKEIQLRLDYFKKILDQIGDFKCSVENILDKIYTQINAQIVTIQKEKSSLLENYQLFYNFQDEVKSLSKFLSIDNQNKQLEFQLDSQLIHQIFQQFELLFNNASYFQTIDTFKDVKQKIELMNENIKFELMESKSQNSQKTTTLNRICQKHNKEILMIDIDTKNKKMEDRFVCVNCISDHPNCQYKTIEKVNEELNYVKNEQDRILKDFKTKKLEKYEKLQQQIAIMRKNQNQQLNEISDKLITEFSKQTNTTDEISIQLLRDEELESSINNLIQFDQKNININQIIEDIKSKDLIQEKEIESKLESLKQQDQLDIQESINILKDIQNENQFQGILQLSQYIQENTNINQENQTVQKEIHELIDTLKLNNCQRDLPNQISHQFQENLKKINNATNKIKSISDLNPLDFQKQVKDDNEIIENDQIYLKKFCDNQNIEQNLETLQIEYEKFQQACQIKIEKLNKIIDQLEKEMEQKIKMKEDEIQQLHQKMKEKSILQIEDQQQVQQQSQLIDENDYVVDFSQIEIEYEGIDFVGFYQQQFLIIDQLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSF6</accession>
    <name>A0A8S1LSF6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0470141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSF6"/>
    <dbReference type="OMA" id="EERVCQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="102" mass="11687" checksum="E8639BD8BEDF36CB" modified="2022-10-12" version="1">MDLLKGGFQGDNSDGDSLRQSWTTEDSLSRTSSYDAAEEVNDLEERICQYLNDLNIQSFTNPTLKEEMYNLKLKQKLFSKIISLSNTNHSTLMKKRSLLFAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LVF7</accession>
    <name>A0A8S1LVF7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ODAD1 central coiled coil region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0480168</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LVF7"/>
    <dbReference type="OMA" id="VIQEWKS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035253">
        <property type="term" value="C:ciliary rootlet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097542">
        <property type="term" value="C:ciliary tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049258">
        <property type="entry name" value="ODAD1_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033192">
        <property type="entry name" value="ODAD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46518">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46518:SF1">
        <property type="entry name" value="OUTER DYNEIN ARM-DOCKING COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21773">
        <property type="entry name" value="ODAD1_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ODAD1 central coiled coil region" evidence="4">
        <location>
            <begin position="143"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="527"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="34"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="104"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="335"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8072020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="546" mass="63916" checksum="3F76F048FEBDF801" modified="2022-10-12" version="1">MSRVQTAKSQQNIRSIEEELEHQIKKSGLLDADRKNFYNNAEETKRKNNEIIEQLKKENRDLKKLQDELIANKRGASASLAKTQGAFISWSGDIKDENYWRRKLDDAKHLTKAKKERLIQLQDKLNEVSDTKFGVPEESPLMRQIRILENNLDKVMIKYNEAQSIRKTYEQIVKRLKEERVGYDNQLAAIERSLKGKEHDYEELLLLAHDATHAKELAQAELKKYEHKKAAVRELRKTYLDAKRKAIEEREKVIQKLEKREKDNVQTQVEYNTSNIPIQESSNPNVDTNIQKQKLADYEEAFHKLKEATGFRDVNDIIQKFTSQDETSKSLSDLKGEYQDKIEQLVTERQKLRDHLNDLKYEGGEALTRKQIDEIENNVNNAINKCERTKLKYERVQKILVNVKAGIEHLYEKLEFFKLEGKPNIVISDETLVDGLSQVVEKMKLVLQLIKNDASYRAQDFKQTWKDFNKEHIDQFINLNLRDNNIKPENVSRNIRVRIQDKEDDDVSDGEIEDDIDQEVTAKLKQKYGAQAKDKNAKKLNKPNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LWU3</accession>
    <name>A0A8S1LWU3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0500272</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LWU3"/>
    <dbReference type="OMA" id="CAMNKLA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012493">
        <property type="entry name" value="Renin_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13351">
        <property type="entry name" value="RENIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13351:SF1">
        <property type="entry name" value="RENIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035912212" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8073228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="46538" checksum="4B07BF2C96BF6D3B" modified="2022-10-12" version="1">MKTLVFIICVVYTFASHAYLYPFNENNAQDLEGLNQKQIAQLIKYNIGLVEHVSQDQQKAYEQLIISFGKGNILTPTEMNYLILIESDKLYQGQESNQQRITEGYAQSFKVLEREQKSIMETLFGQQADSLISKANKLFNNNNLGFKIYQNEDDLDVNYLQWTVTLYLGKNNEVYLYEPKKDKSYKVDQSLEKCVDNFFGTTFDYAPKDNKIYKYNTNEMLDITQDNQDQIIGFLKDICAMNKLATNFKKNNSPNFLSIINKSIAKLEKILNEQEIDLVYDMMRIAHKKLSKNFRNTFESEDLFGLILIEEEKNPLITTTKESAKVQRILMETRTRMLNQVTQTNSSTYVMNATTYQIYVWFGVFFVIVLIGIIYSMVTMDIQKDTLLYAKFLTTDQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZY9</accession>
    <name>A0A8S1LZY9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0500067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZY9"/>
    <dbReference type="OMA" id="HNDKITI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004928">
        <property type="entry name" value="Eng_C102 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="26464" checksum="69A1C2CD0BE35FF0" modified="2022-10-12" version="1">MLSSEWELSIAIIGDSGVGKSTFLSMFITNQFVQRHNDKITISHKEIMIGQKAVQLKLIDISGKEPFRSMAWVHYRTCIGIILIFDLSSRDSYENLKKWYEEIQQYVDQEKIIIRLVGNKCDKLFYDDLDLGKEGGNDRHYIQYEEGEQFAATHSMSYFQTSSSVFSLAQDENYSSIPSILTKFTQEILDFIKKNEENGKIDELMGIRSLKKQVVVQEVKKKINRSSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3V6</accession>
    <name>A0A8S1M3V6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0530100</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 13 14">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3V6"/>
    <dbReference type="OMA" id="HVMKLSM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000140">
        <property type="entry name" value="Protein phosphatase 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8074687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="282" mass="31797" checksum="BE80633ACC6FF13C" modified="2022-10-12" version="1">MGSFLSQPNTQKSHEYQFSGYLSCYTTAMQGWRLQMEDAHLMKPNFIENISLFAVFDGHGGSGISKFLADNFMNVLISQPAFEKMDFMQSLHDTFLQLDDMIKNNEIKNTFIGSTAVVALIVEKMLYVANLGDSRCLLMRDDETIELTKDHLPSNELARIRYAGGFVDEQGRLNGTLSVSRAFGDFEFKQEPLPANQQMVIAEPEIRKIKLNKDDRFLFLGCDGVFETQNSYKVMEFISARVAEKQEPSIVLEQLLDTSLAADTSTGYGCDNMTAMLILLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M569</accession>
    <name>A0A8S1M569_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0500100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M569"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="60" mass="7062" checksum="7B2540BAEA998D70" modified="2022-10-12" version="1">MNNVIDKIENKNLDPIIVMKNREYLIDHLVIEELVKKTKTLNIEEDLDSTIIICNSDYED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8Q4</accession>
    <name>A0A8S1M8Q4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0470274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8Q4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="59" mass="7027" checksum="20DA16799FC65A1D" modified="2022-10-12" version="1">MKGNSNETQTITHYLSHRIFKRKVATQIISFSDAQELRSFKKLLAQKILSDKRNNYQWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M906</accession>
    <name>A0A8S1M906_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0480030</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M906"/>
    <dbReference type="OMA" id="MINDCGY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000200">
        <property type="entry name" value="Syntaxin 11-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF83">
        <property type="entry name" value="SYNTAXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="174"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="19"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8071744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="267" mass="31650" checksum="2A5B338CDCCF9F42" modified="2022-10-12" version="1">MSDLHTSLLNNNISFDQLYEKVKAQLIDYQQSVDELQVSGESEIDQHNVNQVFLQLKAIQLEHMDIKENIIKLEQSSRGVEQRNKTKKLRDLYTSHLQRHQQLYKNMINDCGYESLKEVLKDIERMMKMTSKQDVQNFTLNHSSNNQQNFQNAQQLQMMDVQTFEQFDHIEWHNQIIKQNQEEIDQIQYKTQTINKIVQDLALEIEHQDTYFDVIETNVTTTKENVIKTQEQLTKTQEQQKTGKKKLWCMLICAFVAFLVLLLILLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M994</accession>
    <name>A0A8S1M994_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0480095</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M994"/>
    <dbReference type="OMA" id="MHIRANI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12236">
        <property type="entry name" value="RRM_snRNP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001833">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034143">
        <property type="entry name" value="snRNP70_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051183">
        <property type="entry name" value="U1_U11-U12_snRNP_70-35kDa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952:SF5">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="138"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="242"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="242"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="270"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="293"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8071874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="305" mass="36321" checksum="11B14D6900B718D8" modified="2022-10-12" version="1">MYYQAPTPMMPLAHAMNNPLQVQQAANIRKPDFPPDMQPIFVIPQIAHIKSQLKPKCRPYDPILGIGRKAEWMFDKFEDEYEQMERVEREKDIVKPSRLPPKILKKQEKKRQHSEEVKKRLQTWNPFEDSNITSDPYKTLFVGRLNFATTDKKLRKEFEEYGPIKSVRIVRDSTHDKPRGYAFIEYESKNSVKSAYKYAVDKRIDGRKVIVDIERGRTILKWRPRYLGGGLGEFRRSRSEELLKKTKEDQVEKEEEDKRRKTKKDEGKSSKKPRSRSRSAKKHKKRDESEFKKNKKREKSKKEHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9W5</accession>
    <name>A0A8S1M9W5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0570173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8077020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9W5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="194" mass="22915" checksum="2889B856BEDF34EA" modified="2022-10-12" version="1">MNNFHNYRANRIALKSVKQRSAQKVNQFRASLKSNEPVDSVIYNHQYQREIRIKTEPQESLVSKATSQNQRFNQLLNQVDERALQLSIQIKQERYKKQPKFPPLKGFVQHNYILEQSLDTPTFRNKKAQLSKTLEIENSSTLMDFQLNSHCFPTFNESKEQSPNKDKKILTFPKCIFFCDVKKARNFFQIPSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBF4</accession>
    <name>A0A8S1MBF4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0540197</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBF4"/>
    <dbReference type="OMA" id="NYHIPTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="126" mass="15375" checksum="1ECA37CE6B47FABB" modified="2022-10-12" version="1">MKRQKQFEFNFTEKIKYHHQPLRMSPSFRIKFYSPTKVQKNKLNYQKDIKLYQSHCFGQFIDTYHIPTIHTQRAASQKKAYLYSRQQTPNYSQINQKDSDIENNKFLESSRIIRLKKQIRIIDNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEE3</accession>
    <name>A0A8S1MEE3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0510183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEE3"/>
    <dbReference type="OMA" id="CPTIQQD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025640">
        <property type="entry name" value="GYF_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14237">
        <property type="entry name" value="GYF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="2">
        <location>
            <begin position="287"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="426"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="509"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="579"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="609"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="676"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8073674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="723" mass="84151" checksum="006EC7569B57C4F9" modified="2022-10-12" version="1">MNQTFSKIRPKTTDSKIRNSSHHQLISQLFQQINSYYEQEEKPRPKTQSRQRKMIPLNYSQSDTYDPTDNLIDRLCPTIQQDAKFIFIKTRERALRQPSSGTRRKKMNEEPKFQLNPIKKTTIIQEITEQTAPPKRTIEQKTDHSFYLFYQYAQNNEKRQNEIIQLQFQLTFYFAIKYTCQILMSHPSTLLEYQPAKQPHSNIIKLLTQHQDLFLIQNQTPSTLGAQYPLNLDAKEILIEVQPDQKFTHPKYFQKDNKVQKITKQPKQPASTIFVESGLCDPNKKAWYYLDENQKTQGPYTSAEIDQLITKGTINMQTKVALETLDKLVRVEKIIQAVNKKKEADQKLKEASKLKQEEENQQKSLDSPTKVEKQEEPKVQQSKQKEEQGIDFEIDYTKIKQNIDMGQWAKPLIAQPIQVKVTKAFTNNPQQSKQQQKQTNKQTERKRSDNKSMHVEGNKGNLYQKKGYEKQEEVILEAKPKEESDATINQEVQDYLKKLESKKAEIVKEQIQQQQEKSEKLPQQQQTQVQKAQKLKTEPPKQEQKLQPKQQAETVDSGDEEWEIVGAKKNAQTKKEQQQKQTTKTEQLTTQQKKQEQQQQQQQQQQQQDENDDEDEDGWQTVGASNKDKKKPVNKKIQQKNSNIPGFAVEVKTDSITISSGSAKKPPPQKQKKPEPVQQQQPQQSQAQNQQQNIQNADDDDEGWIEVEVGQAPKKVTQSKKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEG8</accession>
    <name>A0A8S1MEG8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Malectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0610082</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the malectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEG8"/>
    <dbReference type="OMA" id="HTEYIEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039155">
        <property type="entry name" value="MLEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13460:SF0">
        <property type="entry name" value="MALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="757"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="Malectin" evidence="12">
        <location>
            <begin position="493"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="303"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF11721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8079020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="814" mass="94758" checksum="C366E18C967DC668" modified="2022-10-12" version="1">MNNLNEFKERIQFLLAEALNPPKKQQCLIEFNRLLVANKSDQHQKIVLQVLETQRMNEVNLRALKISVQVQKYDLNSSLRAATIINKAFNEGTEVIYQLCGECYRLLANSTTINSLSDLLCNLALKLTSQPIQIGSTISLHYLLQFEVLPNTKKLIQQAYIKNQLICHQSMVEIMIKITTSQDSNLVQILQRSLAALQEQRHTYQVKQTLCKFMLALSDYQIYDDNLLLQCVTILRETTFDRVVTVQQAAKLALQSYQNLYIARNPPQVTTSSYFPDVKPFIKKKRGDGHLVLNAEKYKKMQISQVKQQLEKIQEKVTEQKIQDSLIDQFIQQEQKQSDLQNQEQIEEIQSDQDQNEEITENINPQLQDTQLFVDEKIEETQDFTEDYYEKVLDLVEDCQIDQAFKLALYNNDDFLIIQLMMTVKDYFSELSNKTAEQLIKKFNAIYKSNFVEDAQQRNAQNWINKLIIKIYMIILFFLLQISNNKLIAPRKVIHAIDCGNYIDTRTSSGIKYKSDHHYIGTSEIVDYYEQAENRKIKFTDDQSLYITQRQGQSFAYTIPIGNEVPENQTYVLVLKFAELHYEESNSRVFNVLLGKKMILQNIDILQLVGSFTAHTEYIEIIIDGGAVYYQGEPCFGALNNRNELVVGFSATQDLATIAALFLFKGPLKDTDYEDKQMFQERWIQRNTPKTNDEIKKQNEERIKNIEQEIKEKQNILKIREGFGHEIDIEEIKIPQKKTEWPEFSFQLLFILLKTPLGAVLLVGFLTVCFVTISFVFFDPYGQNKLKQQYQEIKKQKNIKQSTIPKKQVIQKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEJ6</accession>
    <name>A0A8S1MEJ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0600123</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEJ6"/>
    <dbReference type="OMA" id="CTRDTPG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="4">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8078558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="181" mass="21380" checksum="42CB27BB9CBB82DF" modified="2022-10-12" version="1">MCLRDIQQYHRYQRIYNLDRIVGFFINFQLFILNKNMKEEQQNQKNKKKSFTMQNKPCDWICVECRNLNYSFRKICNRCQQCTRDTPGTRFIPNKIDQGLQILETIKLQEIDMGGSSHSSTDSNDDDEDGLFSQALFLEDLSKNSSSVKKSFSFLKKCQVCCNQNYFYQQKCSHCGYSSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MG92</accession>
    <name>A0A8S1MG92_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0560192</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MG92"/>
    <dbReference type="OMA" id="PERQSYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="203" mass="23678" checksum="2DC2ABFDBB527984" modified="2022-10-12" version="1">MIITIIQYNKIQWQLLKLQYLYIKIQVAHGMPSKKEVISSKRIFPEQHNRKGAFQLTWDIQGVFPKIAQKIGVKPVPDDPPLPPKPKYIPPPLPQNYVPPPIKKQGKKTGKPQMIPERQSYEESIAKKTIRQSRKSLSNTNYGKEYGSFYSMDRCFLKRDKEFIKKEVKGYGNFYEKSPDGRFVHVPMPPLSYKYQYVASTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MGS2</accession>
    <name>A0A8S1MGS2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0620227</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MGS2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001341">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="9"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8079840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="501" mass="58783" checksum="18EF9C8DD2050A1F" modified="2022-10-12" version="1">MKRFDLFYEFSNQQLGQGVFGQVYLAKKKTTQEQVAVKILPKNNQLTISEIEILNELVKHSHPAIVRIIDVYDQGNQVYVVQEFCNNGTLYDFMKKKPQFMGEEHCIEILEQVAQGLDYLHKINIVHRDIKPENILRTTFNGQIYYKISDFGLSSIGDMKLTSKFGTAYYVAPEIIDGRTELYGYDKSVDIWALGLMFDELIHGTPFYDGLTEDEVFIKIRNEPYYPRKHEYSGSRLPQKKQIVVNILMGIINKEPNQRKSLTWILDQIRQYKQSVINSSLSLLNQATFTQPISPLLVNDLDVIQDRKSIYQQKCPLHNQPAIYRMPLKEEDKDVSRIVSACSKCLRSIGVYELDSLQKKVDEALRILTIENFDQFFTQNYIKQELQEMRAEYFLNYSYFTIRDDQLQSKFDVIMMDQQKKAKIEYNTKIKQLINFRDKNYTSDEQWLIYVAEKLTDSTENEFVLYIKEDMAQFSEHGIEDCRQLIFNHLKEASEQLSSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MIE6</accession>
    <name>A0A8S1MIE6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anaphase-promoting complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0610233</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the APC10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MIE6"/>
    <dbReference type="OMA" id="FITIEFP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08366">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000122">
        <property type="entry name" value="Anaphase-promoting complex subunit 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016901">
        <property type="entry name" value="APC10/Doc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004939">
        <property type="entry name" value="APC_su10/DOC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03256">
        <property type="entry name" value="ANAPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028841">
        <property type="entry name" value="APC10_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01337">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51284">
        <property type="entry name" value="DOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DOC" evidence="3">
        <location>
            <begin position="7"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR028841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8079269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="197" mass="22773" checksum="DD53E68701F4F25C" modified="2022-10-12" version="1">MHIENPFYSSIQTPYTQTNMAPVANMQRKEIGDQAIWTLSSAKAGNGVDQLRDDNLNTFWQSDGTQPHNITIQFLKKMRVQEVALYLDFKQDESYTPNKLSIRTGTNIQDMKEVQFIELKEPYGWYVFALKTKLLNGQEKPYVSTINIQIVVLQNQHSGKDTHIRQVKIFGPREKQNQGLSFPDFKTPEITQYASIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNC2</accession>
    <name>A0A8S1MNC2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0640187</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNC2"/>
    <dbReference type="OMA" id="ALNCKQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="70"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="104"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="138"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="437"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="539"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="573"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8080832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="828" mass="97014" checksum="82BE8C42D20A535D" modified="2022-10-12" version="1">MGCGSAKPVNVQETEYINSSKNHSRTSSISEGQTETTKLYRRANYLIKNERYEQAMQLLDRVLENDPDFADAIYCKGIVELNQNNLEDAKKYFQISLEKQPNHALALNELASLMIKEKQYKEALEYLEKGFQVNPNIPDLNYGLGYVLARLKRKSEAIKYFDMAINQDPNQKHYYVSKATTLSDLKQYDNALEAIQFALKIDSQYLDGYQVLVYIYRQSKQYEKMEKACEQVLKLDSQNQYALNCQQEIPKKISQQQPQVSQVQISVEDQLKEVRKLQVEQQYTQALSLLNLILEKDPNHLQALKLKAQIQIELKQQASCLVTLDQILTIQQNDLDTLKQKVTILEYLNKNEEVLKCYELIIVQDTSFELLEKKASTLIKLDRIQEASNIYEQLSSQSSKDDSKIFIIRGRLLQAQKKYDDAIICLNDGLNKFPQNLEILNLLVQMYKDTKQEQKALEIYDKLLNIEPTNEQYLYEKGSILFNLNNFNESFDILKQLKKNEYAQNINYYLGICCNEKKEYMDALKYLSLYIKTGKENLEQVYFIMGSANLFLMKFDEAIDSYQNCINLNPNNSEAYFQLGNVYKQDKLMEEAQKSFEQAVKIDPQNNLYKQALDNIINEKSYLQEYRNSLLFTMITFIGSCQLDPKSQDFEKKQNEKYLTLLTLMEAHLKKRFNTIDKHFEQLKELIGKSYDMKLTLIDDTLFPIMQFWHNQKICKKKKDNNDLVVSMINAAKSMINSNNEKILRYIQNEKSLNNQFCIEFLQKCQHESYQKISGKIGLQDGIKILGFLIKYHKEFNSDIKSFAEFITNKYLIDEIQNFHDSYYHQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPQ5</accession>
    <name>A0A8S1MPQ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0670249</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPQ5"/>
    <dbReference type="OMA" id="IGQFAHN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="408" mass="49346" checksum="FE9DBFC25C0F0CA6" modified="2022-10-12" version="1">MKPNQKVLDHLKKYDYYLPLHLDYVQKWFYEQMVDNLGLGESFRTSFLSQISKIGQFAHNEQQFRQLVRDSWFGFLNKKPLNFICIMIDGQEQNAIKEFLLNVLIQPNQYDITYIDELLQIYEYYLQGNGGVVNIINRTKFRMILSKLNCSRFILNQDERIQLQIKQYMFDLYLKNIEKIDEKERINVEFITRTNIFNKLFQVLFDQIEGQGNMFNHLKQIVFVMIEEQQNILISFQILKSFYQMYLDKEKLLFERIDSRVNFDHNRIARELNQKYKEKKDLGETLKSIVQRQTQTINYKLLLRELVTKDIYSKENEQIFNPLLKEFDINNQQKNKRQQYIDNDLIQQIKLLGFNKLDKRNENEYDNLNFSINIIDNNNIIVINKRNNQKGKCQNIKELYLFMKNSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MT64</accession>
    <name>A0A8S1MT64_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0690019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8082919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MT64"/>
    <dbReference type="OMA" id="ECIINQA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF12">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="359" mass="42273" checksum="023E124A67F26A4B" modified="2022-10-12" version="1">MQQIDIDVINCNMLKDNEDSQGILYHTKTHRDQLRDSYFSSRNVENIIKFRRANYQYYQFAKFHQTKANEITHYQLRKCLLYNQFSNLLIYTSYNRLCFYNPCSYKQSIYTISQDFNIVGISQYEDKILLAGLQSNILFFSLKTGDTQEQKLNLNQECIINQADFINDQEIIISDNDNQIRIFDADNLNQEKYLALCSHCVNHCAIKQDLIIACLDQPLVIGHDRRQKKPIFSLQGHQCNNFAAQFDPINEKKVATSGEDKQCIIWDLRKLETPYDKVDAISKPFYNIQYNNQGQLICIEGEDFVQIVDVQNTKLYQIIDFFGQCSGCTFYKDKMFFNLQSHRKMGLFEYNLINNNNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTJ6</accession>
    <name>A0A8S1MTJ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0660032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTJ6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="9358" checksum="3AA84C992D0ABF4D" modified="2022-10-12" version="1">MFTRPIVQQKKLNSQKKDINYFNQLYNQIQVYCIIIAIWLILQSLLQLEIINLRMFLMFKQIMFQKKNLHIKNQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXP1</accession>
    <name>A0A8S1MXP1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0670143</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXP1"/>
    <dbReference type="OMA" id="GNNCCST"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="14619" checksum="5BC342BE0417CDA7" modified="2022-10-12" version="1">MGNNCCSLSIPTTQTEEEIVSSNQQESITKSLGDSLQIANFKELIQDHCQFYTYQDGDILLTSRPISPRQQHVIIYLEPKIVAEEEIKFQQEGMKGILSARTYDQNPESSCSSINCKEKLQKKVQFRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZF1</accession>
    <name>A0A8S1MZF1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0740253</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="TMNIANR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF12">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="433"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1182"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1212"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1290"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1316"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1461"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1485"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1520"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1570"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1595"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2078"/>
            <end position="2100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2112"/>
            <end position="2136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2156"/>
            <end position="2180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2187"/>
            <end position="2205"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="1671"/>
            <end position="1806"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="2259"/>
            <end position="2390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="181"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1924"/>
            <end position="1958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1925"/>
            <end position="1934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1941"/>
            <end position="1954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8085670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2465" mass="289601" checksum="CC856A64CD610290" modified="2022-10-12" version="1">MNSKEKPIKCNIHPQFQLHFRNGEVIKLDGYSTYSNRINRTSSILSIVLFAPFRIARNNIIYLTLSIICLILLASIWSMQFFNYFRDSQQYEFYFIFSFQLFAHISVELYNKYRINGLDIKLNNQENKILKQWDECISNKEILKRIKEKASQKPKNEEIKEKDRIIPIKNDHPIKNDQLQRQLSQKSRSSGFQSQFSRQSINQGRPAGSPRQDRFDRMGSRASLMMSPLIHEDMIIKNELKNKLVQDPAIRKFMQQVPCIFEKNTSRSKKIIDSKIINSTDKMDLIKSITSEEIKIGDVIIVERNQIASCDILVLYCNDENFAISHQLCEYSNTTIRKPLVQNKFDSHNLVLFKKSFTGNIQLNENANNISGYFQLKKDPQSKVIEDQNFIFAQEKLLNTPWVIGIVIQVGLNCRSYKQFKIRPQVPNYSQRLLTSMIFLYFAFLILMYIITYQNKYVNEYLDIISLLVEDLLYLLFLLPHSFRFYFDLIQLFQQRIIYKISQLDMKQIKAFHPEYYQQYKCLTMNLDSVLEGTFELRAIICNQRICHCREQELLQQAQVYASSISSQNIFKTVDSKDDSKIVEIQASNIHQTQNVKQQNNTQNSGRYLEDEIFSYEAQEDMVLNIPQSVGFRLISNFTNNDNTQQELNGLVQNQSINPDKSEYLQKSSINKIQLSMNSKSAKQVSLKDSRVDRKSIYLNDLIKIITNEDEFNNIILLQLSLNHISYSKLRYTQTGEEKVKNTQVNLLDEKQIQIAKAFGFEFICVNNVQNISAYVVQINQKLCSFKLIDFQIHHHTQRLYMLFELDPDFEKPLNAEYILLVREANYKKSDILDENKLNQKSSIHFLHQMDLEQLHQIHYYSCLLNHEDAAYYVNQQSLQYQNTFESEEFIYNYLEPFLQFNITLGFKYNLKPKALEFIRNFEKTDQSLVFYTEQRFSYAYSIIHQIDIQNHFKLIFDQDNDDDLRAYFKKSLQQFTQLFSDSNCVSNSQVQSLNKLINPISSQLSNKKVQEEFHSAPPITIMLSSQACKIMENNIYMTNHMKLLFSLAKITLLYDASNQSLNFVQKLFDEPNLTLNLIFENQTLLKCHQGQHLQIALLQEFSYFQKAKYENVNKQTFFKNMIQSLKVRLVYERCLFNELLIYHNTDAILQGFNELNELLFYYVPLLNILTKYQFLIAFQRTIYFSSFAITIYTTSIWFFNNQKNLIVEFVFYSYLYSLIIYIATTIEFINQQNLNTKKDQNQQKVIEAIINQSKNKSNYFQIAIKSIIQGMVTQLIFTHQSQIQSVDVAIVYCFLSITLNDLISLLLQIGQQLKVAVFIIYSLSYFFYLLLHFNIMAPNLEFKFEISNLFEILLVIFIFQVSEQFFQHHFVLQVPQMSGLFQQIANSVNRHKEFINNKQTSILNLQREINKIFENIEQVDFNLQKLLLSQKQLFQNLGQWQSQIFQKAQSIMKWKKKSRIQGLQLIFFHCQMLIIIYSQSDTSYYFLITYIIYQIFLIVIFVFQLLYHLNSRQTQYLELIKYMMSGIVTLIAVLGLSVTEMSLYYHSFSEIIVTFQISSKLHPIYDNRLYLIPIVVVLIAYFVIFSLNVDITPYFVIIKISMILFFSVQQYIIKSYFILLEEDQATNQINFIEENNKINDILGILLPKFVRHRLNETDQYNIHQNQGHVAIVFCDICNFDQIIMEEQENIIPFLDDLFRTFDKYCEICGVQKIETVGKTYMASAGLKACEQELAYLSEIEPVQRALNLAEMMIIYIRSKLWGHQSQALIGKIGIHYGRAISGVIGFHKPQFSLIGDTVNTTSRVCSTGQEDKITLSERAFEQLKNDKIEFEVRYVEMKGLGQKPTYVYVQKGNTKNYIAKNSMNNSNSNSRSNTMNIANRVRTQSSQKGGLKKRSIAIQDPLTQRSKQLLQFQLSWNYQQQHGQQNSSSLSEENSIKKGLQNSQSSNNLNHLSQDPDHEDKLYRNIKKPELIQQLASAFHQKFHLEEYQPEVDHYIDYDQLLNVILLKNENGLEETLILQNSFLSLLKRSYNSNKNNYIEYHEYIYKQSEQVTNKIIQIYSLYYLIKQFCLIGEYNLISLPLIILQWIGCALNIISLMMYKKRMISYWIQLIYIGLSFQLIIAGIFVIVDMTLFLKYSHIIEMIFIQSFFSNIQLLHFWIKILFCICSFIFEAFVLIFWKEHSISLFFAFIVMVYNINYCYFLEEQQVACFNQKNIFQSQQAKESQLLQYLLPKHILQTFLDDNNRTRCLSDKIENVTILFADIAGFTEYSSKVTPEEVLLMLKNLFVEFDRKCYELNVYKLYTIGDCYVAFGMIDYNERNPAEEAKNIVDLAFEMIRIIEVVRKQINFDGLDMRIGIHTGCVFGGVMGTDIVRYDIYGPDVLIANKMESNGKKGQVHVSEVTKQLLEQDYEDIYFFTLNTKVTLTTINRSIDGYIVESLAEDDFPSDQINSQQIQVQSLHSEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0C1</accession>
    <name>A0A8S1N0C1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0680009</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0C1"/>
    <dbReference type="OMA" id="NTMAADY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8082545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="69481" checksum="58D30EF82981B9AF" modified="2022-10-12" version="1">MIERNRVSSQQKLPTLDLSSTNRGTVDRLKVNITVQSGDAQFISFRSLNEQNPKFPLPHNSVDSSSNLNSSIQNDLNQNKKIVQLLGNTLYRQKYEYQEVQETLIKKMRTMTRMQKYVKRIPHKVETFQNLEKIAQTSQQQIKKKVSFPMFYNGAFFTKLQHQWIPTVRENTMAADYQKRIYIFGGIGSEVFGDMIEYEIQFGKFRELKQKGDIPLNRYGHCLHCIGYPMLLSTQFSYDNQQQNSINGKQLIIYGGEMQFNSALKIRENLSDTRIYDIETETWQLLKPNIECIPESRRSFGSCIIGRGLLIMGGIRSRFQVFNDLHYLNLSLCKWTPLETQKNPFIKGLAFQQLVCVYNKPQYIFEKRETRSKQLKSQDYEGVYCFGGCFIDENKNTQYYSNFLAILKFDQPFNNWIIPEITGKPPMSRIQHTASYIQDMNIIIIFGGRDDQKNNPYFNDLFAFKILDREWIQLDIFGSIPRPRAAHTAVSYRSQILYFGGVNLNGYVEFSVNIAEFNQNQIRQLAAEYRPNDQRHDQELEREEQQPQSIINKQYFSLHQITPVQPLKKLTKAEELDLQIKQLSFQNFPKHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N262</accession>
    <name>A0A8S1N262_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0770047</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N262"/>
    <dbReference type="OMA" id="DGYFLYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001126">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000818">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF136">
        <property type="entry name" value="EIF-2-ALPHA KINASE GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="202"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8086690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="530" mass="62385" checksum="55063EF8272D2612" modified="2022-10-12" version="1">MDQKLLKNQQKRNVNLNQLFNNLIKHQFKRCLYYQFTFIQIQDDSLQKRQEFIQSVECKEYSQMLIISQIRMDFTNDDPFLKHLMNVEKAIKKAIRNILLQLQKLDNKQQFKDACDKLIKIFYQDINIGFITSTGQIKSTAYAFISKSIIELINRKKINLQFKKIPLKITLTFFAIDSLQQINPIQKQYDPRTAFIRNNKFFNDYLILSQTNQDELRSGVSIFFVYRPEDDQIYALKRSKLQATHLDYFSVFSTTASNTPITPDIRKIREAKILSKLTHPNILRLFAWWIESTNDGYFLYMQLEYCSFPGYKYQPTDLLTFSYYYLNVMQNQEKINKIKSILNQILDGLEYIHQRGIIHRDLKPENIFVTINIKGDLQVALADFDQGKDVREEKLSIITDERLPEEELNSIKSQNTITTGTIGYQTANYNKDSHYEMADEFYAIGIILLHLVIAFPGEPKNRNHYAKTFVMADSIKDVLSLFDSWANKFVKNKSSDFSFTHYQNVMELAKLLISSRKLTHADVRKMINEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4J0</accession>
    <name>A0A8S1N4J0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0710104</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4J0"/>
    <dbReference type="OMA" id="QSEIYYH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="398" mass="46950" checksum="1135002A981ED38C" modified="2022-10-12" version="1">MGNALNDIGDNYDFKFIQKVQHSLFGQIDILECQIQDFPDIEQYMRVIPKLNNQDYIAFIKRYIKLQNLHKHELTSPFLIYISNNIFEYPSHSLYDEIQVKRQQGTQFAESELWYLAKNLIAGYSIYLQIPELVKQTQLNLHNIFITKKGHIKLSLHTLFLINSTNFKNLYIEESQYSHKISKEIGLILLEASCLSFEDSIKVMDMYYSIEYSNFVKDMIGDTKSLQYFQNYQMSKKISKTIYGESQLKKTTTLLAQQTKSLKQSQIIETYHIKSPVKTDPNQFDQVSKIASIPEVNEQDQKKSQQLDYFSGRKSTQPIQNIQSEIYYHSKQQSLQINDQQPNIIQFIDNSNLKLNTNQPVIQNFDVLDEKFNQRFWKAIDQAKRQVLIYEQRSKQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4P6</accession>
    <name>A0A8S1N4P6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Anti-silencing function protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0720219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4P6"/>
    <dbReference type="OMA" id="ITCIYHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006818">
        <property type="entry name" value="ASF1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040">
        <property type="entry name" value="ANTI-SILENCING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040:SF0">
        <property type="entry name" value="HISTONE CHAPERONE ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04729">
        <property type="entry name" value="ASF1_hist_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8084706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="23278" checksum="5D02A22FACEBE7F7" modified="2022-10-12" version="1">MAFVSITNIVVDDKSQPFTSPITMDIYFDVIADIEDEIEWTLLYIGSPKDEAHDQILDQFSMGPLTKGTKQFTLESNPPDWKKIPQDELLGITAFILTCSYRQREFFRVGYYVYNTYTSPENIENDPQEVIIEDIARQIFNNKPRITRFEIDWNNPANSDVQQQPIQTKAYMFQEQQEKQSADATEVYNAPHQIQNVFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N502</accession>
    <name>A0A8S1N502_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0710189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N502"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8084125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="109" mass="13262" checksum="C1533B2FFAE70691" modified="2022-10-12" version="1">MHILQKNANTKPYIQIAQSLILKINQLQSYHQEILNFLGFEYFQIKNVNIIESFNINSRNMKIRMQRLQQNIVNYFIDKLDFIYIYQIAFIKTIILQIEQFYNENILKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6K6</accession>
    <name>A0A8S1N6K6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0750015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6K6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8085721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="273" mass="31440" checksum="7BB22B30E2688A90" modified="2022-10-12" version="1">MQILHQTSLILLKQLFESSLSLLFGKLPQESQLPPSTHQVGEVELYQQNAFFKRISKMLLFMLVSILDPQLYYHYYYNRQNQSFLIMFIIYVNGTISKLVGDEISAHVPSLQVVPVEYLYNQLLSKFDQARQYANLNALQLKSLFESKLSEVGIAKTLQSLLIASSHLLQSIFILQQSLASWILFQQENFQILLQFRLEILKGKPSEKTAAQHFQLKYKNPGLWVVYSSQLEAEIISQQAEIASHALPVLQTHFIYSAYCVPNFDESLITFNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9Q4</accession>
    <name>A0A8S1N9Q4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0700008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9Q4"/>
    <dbReference type="OMA" id="HAKRFPM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="26135" checksum="6308062C1D7BB6D9" modified="2022-10-12" version="1">MDHINKDNLHDLNKKLTEENQRLSLLVYGLQRQLQNAAVQINYVQQIEEKLELLQFEIIQLKKDNQLLQEQTFIYNEQQMQKDQLQLQLDNMKSEYEQKLQFLIKENKRLGRLNKEIQTKQTCSLETQAQKLIELGNDLKNKLETFNQQSNNLNQTMIGPKGLTKKIYDNLIQQLQTTDEKSFQITHAKRLPIIQVKEVKQQPYSQKSLVSIPIISSITSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAT8</accession>
    <name>A0A8S1NAT8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0850007</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8089920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NAT8"/>
    <dbReference type="OMA" id="TICIRID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="746"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="911"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="950"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="991"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1013"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1044"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1077"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="416"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8089920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1137" mass="132368" checksum="FC45C0C60680B728" modified="2022-10-12" version="1">MNDYDFLIYKTIELNPHYLLFLSFKFWRIDQWDNKYFSVYIDNKLIHGAVYSHTSSPSDLCGSTSYNDEYYSISGIIDHTSTTVFIVMLAQRGIWGISELEISIEQCPIGCYSCNKDQCLDEYFSSEGWIKDGMVQTSVNGCLDWKYGNVNSGKNLMKVLKLDQHTAISFQLKVLIFNLNPINVFIKIDDVIVFQTEYSNGWININDDEELCVFMQMKNIKIHQYTHIKQQIQISVLLNENSKFGIRDFQLFLGTSYFQNMCTDYNFLAFDGCFSNIYDCVEGCSNCIKGECWDCQEGWEYNRYLRICIPICGDSIIINYEQCDDGNQMPNDGCHLCIYSCIQHCLLCQFGICHQCNPTYRLSADETICIRIDDQDEQNFIQTQQFQSDLRLCSIECQICQECSDIKENSCYHICQQKIEESLETVEENKEEEEQEQEEEEMDENLNIECMSYCLQCADQYSCLQCEVNFQLENKQCFPICGDGIIIQGYEDCEDGNNDPYDGCYKCQFQCSFGCISCEQGNICRKCDNFTILNNDTGKCEKEDIYNANQFTEQNNTNSSSILGIQNSILDDNLCGNGILNNNYELCDDGNNVGDDGCSSMCLIEDNWNCKNQFYQSICFPKTQLLLTYLNHSNSYQYIELSFSNQVKTIQSNVNFIDCIDIKLSTKKYSFSIKEVVGISQQIFSIPIYQIQINFYDSIVNPILSISINSKLKDINNFEVSNNKKNITLQSVIILSSIQKNVAVKIYNFGFWMMIGLGSGSAFLILFGEILQFSEILDILQFQSYQRFINVKYPENMEIYFQTSELVTVTPVLMNLKFIDFFDKIIGYQFIDSVGKLHEYNINSDLLFNIYGQITQIFLLIGFELFLRFYKKVFISQVFDVKQIGQKIKIIKQIRILNWFKLKFYMIGMKIFKLKSILTYQVFTQIFYANSLDLSFKTINHLLSNNDQNIRSTISIFISIIYLFMAILFIIKSLRRIHSKHQLHYLRNEQHEGLLILKKLLFSLILIGMQTQPAIQCLLLTFINFCYLSFLINMKKYSKNKNDLINNIWVEVPVMIVTFLNIPYISEFRKYLTPNQYINLGFTQMGILIFGLLGPLVRCSIQFFIKFKAIKTIFPQNQIKKQPSRQNLNIFQMEQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCP9</accession>
    <name>A0A8S1NCP9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Splicing factor subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0720118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCP9"/>
    <dbReference type="OMA" id="YDRFNIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009846">
        <property type="entry name" value="SF3b5/RDS3-10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978:SF0">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07189">
        <property type="entry name" value="SF3b10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8084504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="10400" checksum="961FB65F9B29223A" modified="2022-10-12" version="1">MQDRFNFQTQTEHLHARYVGTGNPDTPKYVWNNHIHRDTLASHIGHHSRMVYFCTAENEPMCRLRYKFLTKMIQPCGPPPPDIEKAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEJ2</accession>
    <name>A0A8S1NEJ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0820065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEJ2"/>
    <dbReference type="OMA" id="RFENIFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="337"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="383"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="178"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="562" mass="66407" checksum="37036FF43BFE06FB" modified="2022-10-12" version="1">MEQVFAYAKSQYCLKHQKQISLVNLDVASEKKLDELLLCIDCQNDSAYIVTLQEFEASFNRNDQNFVPIEVCDRPFERIKMLLNEVSVKLQNQIMKVQKQLETQRRALYQKTLENLIKQSFSQENTNSQIMYNEIGKINLDFSTKIIKSLQDLLNLEEFKRCKQEIEQLKNIFDDNYIRNYQQQYQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQSQSQLQLQSQQQKQLLQQSQLQSSQQGFIFENREIAQSEICYSISFNSTNTMMIAGCNNYIKVWEFSFGLLSFLETLDAHKYTVTNLLFSKFKNNFISGSKAGDLICWKQQNNQFIKSLAYEYHQQGISCMILNKEENLLFTADMDRGIIIWKAFLDRNELSFQKILKNHTKQVNGLSLNDSETILVSCGLDKQVIIWQKQQNDWIFKKNVNQYIYEFGTRIKFISHDQFIWVSGVKNGQDKLFVFQINNFNDNVNEENNKQLLESNKYTDSLFFPIFYLDQKKLIVAKHKDHIYLIKKQDNGFLETVSLKVLNSNEAYGAVTNDGQYLVFWEQTAQRFEVCSIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGQ1</accession>
    <name>A0A8S1NGQ1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0890116</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGQ1"/>
    <dbReference type="OMA" id="TGSELMY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8091898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="110" mass="12553" checksum="17DE658A2003A932" modified="2022-10-12" version="1">MVSCAQYCMFISAPGCIFMTYLGLMIAIGSETVYVVPHSRIEGAFALFITAVLYAIFFAISYNSEYSQIQRQQNVDEWINSQEIREAELEMQDIPKQPKSNLQESIEQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJR9</accession>
    <name>A0A8S1NJR9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0900090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="142" mass="17022" checksum="9C8D47D59A510E7F" modified="2022-10-12" version="1">MIDLIIKPVFYFEIAVIKLYNQNIYYKSKESAIHKLYYNSICFLGILNTSLFSQQNVQLYQPMENVLKQIIKLNTQIPSFQQAIQSNFANMQLFQNYQYEKYQYLFGNNNLIIIKLIDVIIRFNNQELIDLLFDYXQLIKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKX2</accession>
    <name>A0A8S1NKX2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0840059</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKX2"/>
    <dbReference type="OMA" id="GQSIICD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37473">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37473:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="247"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="273"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8089675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="285" mass="33914" checksum="CE16AB04F818810D" modified="2022-10-12" version="1">MLPKLHQSSSVQSVRTSEDFSLNEVKRRQKQLELESQTVENRIKQLKKENEKLSKKVEMAEQLALDVYASRLAQKMKKEQKQHQLSMIDPTKYQQTQYERMHLKKMKQEVREMKHQDAQLMKNSIKFEIMRTNEEQIKIAQNLRLRATLKKEEEKLSQAMIQEKLFEKQQKVRLEQERERQKIIMENEKYEYSIKQLEAQELKLVEKLQATQQREQAVKEKLMAATRLAPEDFDKTYLGGQSIICDQEKQEQKQEEQKQEEQQEQKEQQQEGGQSGEGGQGTQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NLU6</accession>
    <name>A0A8S1NLU6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0790013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HLAFYIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035946679" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="2814"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2358"/>
            <end position="2379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2421"/>
            <end position="2441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2468"/>
            <end position="2491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2511"/>
            <end position="2529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2549"/>
            <end position="2574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2624"/>
            <end position="2644"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2682"/>
            <end position="2706"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2726"/>
            <end position="2745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8087594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2814" mass="330698" checksum="AB443ED83C13AEB5" modified="2022-10-12" version="1">MILISFLVLSTKMLNGFETIKMNPAYQYQRKFIQLTENEYTTNTCLTYGLWSKYNPLSTITQVGMYGLFDSHCFHLHNAIEQNSQSLNLIYYDCLDSVSRKIIKKILFINNLDEQVRLQIEIDSFDYENTWYYLQLIAYPSQNIFKLIFIKMQEVLFQTIKSMKYPFNDENLILSFGGNFRVHNSKIHEIKNGEIFSYFPGTIILQEFSIQSLTLDFAFIDIANQAYKQFDQCICQPNEKFLIGDKDFKQQEYDIYFSDNLNCESFILIGWIKIKEIINPSGILNYQLMKISPNLQNPIFQNQNLSPFQLFYHITSLQNEIEIITYSYTFPDVSIDFSDNPFLIKKKLLIKNKINIWHYLKVELLDNKLIVEITFYEGQEIFYYDAQFEVNHFHNCLFKLQYGNCQQTQMNYLNIQINNLEFFNCYKEITTQNCHYSCYECDGPTNQDCLSCSIESQRIYIQEHKVCICPYNTIDNQNKCQTYVDSNLRLIEDQNLNNNQNCKYGFFEFDGNCIQCPSIIKDDFISCLECLNNPKSWFEYPICQRVLVIKPNTSYYEALLLFGDVQYYFDGIQLNSIHNTYSFSYETLITNITGIYKEFYLSQNYFRQFCQQMDFVDTDQFICYECFIKSCQICQVTPTTFICVKCYGNYKLINGECIFQPSAKAKFELICLPPYYYSFDNYCKICKIKNCIYCFEYSTTNQNFCSLFKLPSLNLNLRQNFKIGCALCEENYIFDFTLELCLRQIPEIMNCQRSYINFQNQEQCVSSMNDDFSIAPEISNCQKYIVNCLICSLNVDLQIKCIVCQSNFIIENDQCYQNEELDVQKNLIYNLQNKIQSFILQFVPQLKQYVYNKFLNQKKVIECDPQCILCDQISTYCKFCPLNYYKKQIITELSNTCTYCHPFCEVCLHRSNKDIQRNYPLFIVNDQNQIYTKQCIKPYFDPSFSYDPYSQYVRYCFTKDCKDQFVFDVSYESCDFTRFNRYYESKINTQYCNQIGIEYLTINFVLKISNQQCFLILPFNIQTELKQKVINLKKVNLKFSSSQYLDIISFNFKPFYNFDQVEISNLGFVIDSNQHFIFYNSNTKIDLILTNFTITQSILQNIDSLFKTEQFGNITLNNFTIINTTLINSSIFNSQSFQMRGSINIINLQIKNCTFIESALFKLSQIESLLSYKYLIIDQSNLINSSIFTFYTTYPQLRILNGQNTIIKKNKFYNSYLINSTYLIQVNLQDFQLYSNLIDTSIIISVNYNIKMNMISIYQNVFTFSQFLSINQILLKNEILCNIIDFEAKQNQFQTSSLILIFSTLSTNFLIIKFSRFSINNNSKYSNDNGKSQLFCMNSKEIHISNFLINDNDDLIIFQLSENKNITIVNIIYQHSIQNFKIPLSQPCLTTNKKNKLLQVFGFTNIYIENVYILKIISVDEPIIQINPSYQNLSYDMNQIEIINLTFAENILIQSTLNNYISLLIIEQDNRVNIILENIKYEENFLHSYSSSAIRTTISLLYIQSSLSQLKVQNFLSKNNALTNSTNSFITVMSFNLILSNFTICNHNFLSQQLWVKYYELQFNENLNQDNLNEIVFQILQIKNTGGVGQFLVQNLSCFNCTFSKILAMQSLIFEITTTEEGYIDLQNITINQVQNNLLSTEKGTGCFSIYSSNSKLNLRLLNAFLSNILNRMASSIFTIIPSKQSNIILLQDIYIQNCLSLVNQIMNVQFSSLIANKNIITIKNMKIIQNYQVWIDYFAKIRDLYIQEITDTINNQNSLISFQNCIILIQEFFIEGLIINSVFQFANIPKLQLYNIKLDKIQVLYQFNLIQVIQLQQLQSIIVIDNLSIKNISLYQNNLIQQSTYQQQSYNIRGCKIVQQYIEKYQRDYIQTIVSSFQQNNDKESSLIYIQSISNLNGFYFRDVTFEKNDCQQCQKGLIFFELSDFKFIKFEKFNCNYNQIKMFGCLHFSSNTYIKQKVLIENSNFLFNNGTQGVGIFSQKVSLKIKQCKIINNNAKDLGGGLYMQLESSGFIVNRTIIIGNIARIGGGIYLDGNSNLNNNNFVSSLLLFNTAQEYGNNIIEIPTHLAFYINYMENPSQFVNINNQQINILHLKTYRMIEQGTQIFMKDLLIPSNQVIKTFKIFDIHQSKYIPFIQDIFLSYKNSRNEEMHKYINSSCKVQNKIITKDKQVIVDNKVSQILIYNVLTNSFDFGSLSFSLDPYKQEFNYLQIDISCQLIESNNTLKYRIYTKSLKCQLGEFYVNNGCQICQYNQGYYSVTYNATKCSIFDKSKYSDITSNMIKLLPGFWRPNNFSDYVEPCFKNPLFCIGGWQVGDQICSFGHIGALCEECDMYNIRGQGNFYKNQWDQNCRKCQFDWSSIFSFMLINLWTFISISMSLRSISKSNQLYSQLIIAQRFSKILFKLNQDQQSIQLKMLINYLWIYSVIFTFNIRFSFSLFFIEQTSDTSYFIAKDLDCYISSIQSVHILYLKIFTMLILMIILFNLVIAGAYLHTLITKQKHDISIFSNTALYLYVFNYAGLIKMFSSALSKREGDVSLQYGSQIHQKWMYYFIIPGIFIIGIFIPIFIYILLMLNKNKLDKIKLRKHICYLLNEYKQERYYWELIKLFKKSIIIFIMTNFETEIVLKASLLGLGLLIYQILAIFNKPFTIQKYNSLDLQTAQICSISIFLALTKSICEQKNYIFPSILIQIFIIGCFIKLCYPFVFGIARNYFKKYQFFYLNKLHFFLNLKVPNFYFAIILGKFLEKEKLRQNKLKYNIAKLKNHLIFLSKVQLRNSRQIISPQSSRILSTKSSRIGVEKVFFNNVEEYESNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPJ3</accession>
    <name>A0A8S1NPJ3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0980057</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000004">
        <property type="entry name" value="AdoMet-dependent rRNA methyltransferase SPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024576">
        <property type="entry name" value="rRNA_MeTfrase_Spb1_DUF3381"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF13">
        <property type="entry name" value="PRE-RRNA 2'-O-RIBOSE RNA METHYLTRANSFERASE FTSJ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11861">
        <property type="entry name" value="DUF3381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="8">
        <location>
            <begin position="23"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3381" evidence="9">
        <location>
            <begin position="240"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8095207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="371" mass="43108" checksum="85083EDDC64587C3" modified="2022-10-12" version="1">MPRKTKARSRLDKYYNLAKDQGYRSRAAFKLFQLNRKYNFLNNARTVVDLCAAPGGWMQVCAQIMPTSSTIIGLDLVHIKPIPGCKAFTQDITTPQCVQLLKKEIPQKADVILHDGAPNVGASWAKDAYNQNDLVLSSLRLASQFLKKGGVFVTKVFRSTDYNSLMWVFNKFFTKVEATKPLASRFVSAEIFVVCLDYLAPEYIDEKLFDSKHVFKDTETDMLQQQIQKEIVSVDKILQKRTQRHRSGYADDVHQTVYQMIDFEEFLHADNPYPIFIEYAGIKMTEEAKEKYLGLTKPPQDYQILMEDIKVLGKREIIQLLKWRSKIKHFLSKQKREQKQLQQQEEPQXLLLFNRKANIDQIKKTSIWKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQG7</accession>
    <name>A0A8S1NQG7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0990025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQG7"/>
    <dbReference type="OMA" id="QYHSISI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="272" mass="32283" checksum="BAB809D67C3DBDC1" modified="2022-10-12" version="1">MRNNSQQKENTSQLKETIHKLQQRDATQRFEIAKLKKLLVERNQEYKLAFDQLQELKRIQQRDNQYILKVDNYVAKLKQKLQKANIKIDSLESKFSSSRVKQNITIDDSFNGNDSFQTDHLTISTKHPILKQTSNRSNKAYSVNKPKLICKPIKVQLPYPNISYTENQEILENNSLNKSYPLQEQILNRILDDEEFINNFSNQFDQMQTDLFSYQKIQTQPLSFSCNSRQSNFSFVNSEKKQNRNDKQQKQPNRQYHSISIEEKENNSKYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQY4</accession>
    <name>A0A8S1NQY4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0940072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQY4"/>
    <dbReference type="OMA" id="EMTKQSY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="447"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="169"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="237"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="353"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8093819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="560" mass="67466" checksum="E8363665E94917DE" modified="2022-10-12" version="1">MNKYYLDSEEKQFQQSNREVLKQIDVNNRSEASLKTSVVSFACDRDNKYDLEDYRIQLEDQLAENIELKRMLMDEIEKNKRITDIDKSSQYLEQQDKFAQILNKKDNEIMNLNKLLRQLEIENKLLHKDDQLESSKRMEKEKQQEKRIIEYEQKINLVLGENSKLNQILQERITQIEKMKYTVDQLEREKVQIVEKYEILIAEANNLSSREKCTIEKCLNQQKNEFQKQLNIVNTQNNQMSNILNQQKNQIEQLELQLEENQVQLDTTKQGYDESLKEKENLIYQKEKENQDLMVKFNELEKKLIQQNQQVTQLEQQLQTNNDQVVIEEMQKYFEEQYDIKEKELEKKHQKEIQNLMTQLNENQNFIEELQNHLTEIHKQITKFKQEKQMLRQQNQELQQQVKQLSSEIKQQENLRYSQEYMLPQFNISCQSAITDQSLCDDKEKLNKQSRKLNKSKDTIQQNKENHEKRDIKSIQYINQLKQQSQQNTSYKAQFQQKVNKTNSQNKDSYALDTFGNRDRSISCLDMINKGIKFRESFDEEDQSPIAQFKQWKQQMTVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NRP6</accession>
    <name>A0A8S1NRP6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tlde1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0920024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8092911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NRP6"/>
    <dbReference type="OMA" id="RTEIMIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021225">
        <property type="entry name" value="Tlde1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10908">
        <property type="entry name" value="Tlde1_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035775720" description="Tlde1 domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Tlde1" evidence="2">
        <location>
            <begin position="56"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8092911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="187" mass="20733" checksum="E4FBD43E36228A78" modified="2022-10-12" version="1">MKYILALLFVAITYAGNCGQTCCQDLPKRSPMTFYYFINTGRFFGGSGDSSISTFGYSGAGKYRNDPTAQCLKNQGPAPSNTYTIKTCQNTMHSPPVDRPCSFWIDGNDQDKMCGRTEIMIHGCACCTPSDWSVPPIDGCSAGCIIINEENRKKLRVGDTLIVQDYDPLSDNIQLDDIIIDKEIMYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NX58</accession>
    <name>A0A8S1NX58_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0960074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NX58"/>
    <dbReference type="OMA" id="FKQKAND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="457" mass="54264" checksum="7B0F8EEC8BEA02E2" modified="2022-10-12" version="1">MTQNLKFHKLNNALPNIQISQHTKIQQDYQDGCSTDRVINLVNKVKAKLKLVNNTPDARFNRQLQQFRILDTFVDTKTKLKKMNILNNRDLIESVKNYLIRRKIARQLGQEEADLEKIYEQYEVKFETEFDKAFLDSDCFLDLGLVKAPINKTKVLSQEKLQDFGKRAISQFQTSSEQMSSRDQVTKSQYDQNQFYYYVEDHIEQLKKEKEETENFIQKLGKTTKSTTHSGRGDEKRSTLIQQQKITQIDYDKELLKIVLQYGIDLNSTEYLEEEIYSAFKQKANDFIKSKINEAKVRHNHNIEEDQLKNKYQIRPQKKRISSMFEQQFFNFSKNYPPSKSRYQPESKEHTLTSGDRKSQILQERMDNKKEKTLLLSQFNLKSKRIINMCNKDEQKLLYNEFIKDIQLMNLYLDEAITRTNFNRKLSQLGFTKEDQFLIKKQIVLPGSSIRNHYDNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXA5</accession>
    <name>A0A8S1NXA5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0890064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NXA5"/>
    <dbReference type="OMA" id="SHWNKGQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055325">
        <property type="entry name" value="CF161"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24274">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24274:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24569">
        <property type="entry name" value="CFAP161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="315"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="351"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="482" mass="56728" checksum="EFC8731271DFBE05" modified="2022-10-12" version="1">MHNYNVDHKYKFQTRIGNWFEEWELDETKKKDYLKNRQNGQLASIVKDQKTNFSLRLASLTFPKDEYIHFGFHIMLQNLKTQGFLSIDIQEKLKIAEEAYNVTTAPATQPTVRSVFVVVPYTKEPNFFGDDVLHYGQHFRILANPRISNNKTFYLHSLPQTPTRCAKISRKQEVCAIESDVFNTVWKLEHSDPKIRFEMEGQPIRIDDVVLVKHSFTQHWLASDDIVYQNDFGREREVFVHSYQCLNKTQNLIAEKEGRTTIDIPLRNQEPQNLWKFIVATKQNEQFDESVMDDNRNVKNLMIRVKQQITGKGSYGLRGLAKIFFEMDQNNNGVVEYDDFKWGLRNFGLNLSEDETKMIFQTFDKNNNGKIEFNEFLDAFRLQMSDKRLYFVQRAYASIEQKSGKVTLDTMGRTINVKEHPDVLKGYKTERQVFQEFVSHWNKGQPDKVISFQEFGEFYQDVSSAIQQDETFEAILKKSWNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYE2</accession>
    <name>A0A8S1NYE2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0970025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYE2"/>
    <dbReference type="OMA" id="ERYHNDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="244" mass="27900" checksum="6E55184E03A27D92" modified="2022-10-12" version="1">MFILVLFGYKQYKVFTIDVLGNTLLDCIWTQAIKEITKLLTAKDEQHQKDQKTAQQKISSNEKRISAINQQLQEEGLLEAPTGKKDDKKGAKPPPQKKGKDAPPSNPLEAEKLELQKDTEKQQEYVTKYTDKLNKIKVAQEKFKDLNEKYHNDQLVIELCDKTGERKFVKTKLDGIANTFLSDKGTYILAYLQAPVTPDEEEQLMALEFEGYLARTVDEDIGAVEEPLLQLQPKKDDKKKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P059</accession>
    <name>A0A8S1P059_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0990034</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P059"/>
    <dbReference type="OMA" id="WRSTNQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431:SF14">
        <property type="entry name" value="LD15323P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431">
        <property type="entry name" value="SORTING NEXIN 17 AND 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="210" mass="24680" checksum="3BD9975E94AE4FE6" modified="2022-10-12" version="1">MQENEFAISICVPSFKKNDDVVYYQLVFTQNNQRRSFSVDYRYSHLKKLYESLQTLKGSLPKFPPTNWWRSTNQNDELIEERRIQLDYFFKTLLLCKDVSQSLIMKNFILKSQCLHLKKIEKEQLILTKKEREAGATPNMSQKKAKSQLTTPVCGPAEDPIEDPRERARSWEFKFTKKKSESMCIKDNNQLNNLTFGGVPIFKGIILRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P388</accession>
    <name>A0A8S1P388_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">T-complex protein 1 subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1040058</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P388"/>
    <dbReference type="OMA" id="GMILRID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000005">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="310"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8097472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="334" mass="37143" checksum="B017441FFFE27EEB" modified="2022-10-12" version="1">MINKDVTHPRMRREIKNPRIILLDCTLEYKKGESMTNMEMTKESDMTDALQQEINEVALMCNDILKHKPDIVITEKGVSDLAQHFLLKGNVSVIRRVRKTDNTRIARVSGATIVNRPEEIQETDIGTLCGTFEVKKIGDDYFAFFVDCQNPTACSIILRGASKDVLNEMERNLHDCLAVAKNIYQDPKLVPGGGAVEMEVSARLLEKANKVEGLGQLPYKAVAYALEIIPRTLSANCGADTVRVLTELRAKHSETGGLYFGVDGNTGKIAKMNDINVWEPLSVKKQVFKTAIESACMLLRIDDVVSGIKKKQQQSGRQGGEEEPQETFGDQRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6Y9</accession>
    <name>A0A8S1P6Y9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1080008</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6Y9"/>
    <dbReference type="OMA" id="SIWAWIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="7">
        <location>
            <begin position="113"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8098800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="203" mass="23074" checksum="974590E294F0453A" modified="2022-10-12" version="1">MIQRIQDYRRDSSNFKINKENLFKNDLNQANSKIKQNFSQNKIKLLTNLMSNTNMNKPMQQSDGDEIFFHIQNNAEQYNIQSADEKNQNIKMIPDQVIESQTTKPKPLTFDQIKLVSSTQVSGDGHVKSVRVQCPQCKQKVDTVIIRKPGTQTYLASCILLLCSFGLVCVSCLPCIIDDCKDVLHSCPQCKSPLGKTQFKILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P734</accession>
    <name>A0A8S1P734_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enoyl-CoA hydratase/isomerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1080083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P734"/>
    <dbReference type="OMA" id="AYRNNEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003860">
        <property type="term" value="F:3-hydroxyisobutyryl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045004">
        <property type="entry name" value="ECH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032259">
        <property type="entry name" value="HIBYL-CoA-H"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004127">
        <property type="entry name" value="PRK05617.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176:SF3">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16113">
        <property type="entry name" value="ECH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl-CoA hydratase/isomerase" evidence="2">
        <location>
            <begin position="28"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8098932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="365" mass="40851" checksum="C0167CE543B97414" modified="2022-10-12" version="1">MRLRLISAMFSSSTSQVLTNRVGSALNIKLNRPSQLNALNLPMIAELNSILDQANSASCIILSGEGGKAFCAGGDIKTLYFAKTQPSEQNPPSALKQFFFDEYKLDYRLATLKPVLVALMDGIVMGGGVGMSIHAPVRIATEKSVFAMPEAKLGLFTDVGGGYFLSRLPYRLGYYLGLSGFRLKGADLVHAGLADFYVQSSELQNLISKIGTVQSQNLNEVKNIVKQFQPNQIPEFTIKQQLQNIEPGYSGESLEQIFNNLQNHQNQKFGQDNLKILKDQCPLSLRIIFEQIKRGQKLDLKENLKMDYRIVRRIMQGTDFFEGVKQVLVDKNHVPTWSFKDALTIPQSEVDKYFAVLENEEELDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7H2</accession>
    <name>A0A8S1P7H2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1080031</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7H2"/>
    <dbReference type="OMA" id="LAYRRVM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="79"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8098828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="296" mass="35607" checksum="0DE44C314C32F3CB" modified="2022-10-12" version="1">MSEKFISKKHNFENKAKSQNQKARFLNDKEESEHPPQMRNAQRFFRKPKATQEMIVIPQPSIQYAQPYVPKPIPIPEAIKQIDKKIQAYRKVMERERQEKLQREKQELQKLYENEEDHKKEIDFMTNPQIIDQQEEEPSQQEEDNQELLSEEPRVQDQQQITVCEKTSYSEQVKKREQIYDHTLQRMYEKRQNPLLFAKFKKKPPILNITTKNKIQKGNGRMDPEEHKLRVYNRENVPTIPDLIIPSFTIKKDQMAKNILAYLKEYTPLFRGYKNYEYPKCVQNILQEEIDSLPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8K3</accession>
    <name>A0A8S1P8K3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN motif protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1090153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8K3"/>
    <dbReference type="OMA" id="HKWINGE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="49004" checksum="B4BC9849137559A4" modified="2022-10-12" version="1">MIDNQTLQSILNYQNITEGYSYGELSQLKNFKTVLSNNKIYFGQVFNSKKHGKGVLWSKSGHIYEGEFNNNRKHGFGMELFQQKSSYCGSYLNGRPEGLGKFIWGNGESYDGEWLNGYKHGYGTWIGLKGDSYSGQWAYDKPHGQGIHKWINGEQYQGEFKECLKHGFGEEIFSNGDRYIGMYQNGTPEGEGEYFYSSGAYFHGQFLNGFKSGYGEYRCANYSYKGYYQNDKKNGEGELIYADGSRRKGKFYNDLFENNETTKLTINTQPENSPQNLYNITNSKTPFYLSQTKPIQKELNSSSINKTVNITRNIQESKFQKQLNLLPIMNNKLRSNSTRQSNQDSDKILYINQYKNNQSVNSKQNSRKTSVVTQPIAITQTIYRSKNQQDFSQKLESTIRKFHYKTELKGTKNQNHRSVSSSKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PAZ1</accession>
    <name>A0A8S1PAZ1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1110112</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8100064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PAZ1"/>
    <dbReference type="OMA" id="DQYQNTF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="304"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="141"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8100064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="353" mass="42148" checksum="17DE9EE747D12B8D" modified="2022-10-12" version="1">MDQYQNTFQMLGQNMFSKAPPSGYSAGYHPIIQANSNKQKFDQLIQLMQQQEQTVSDYNTRMKEKEDHLYYLEQQSMQLQRDLIQKDKQRNEHNQIHYKNYSPTFNVQNTVPMEPLQTELQSVQNLKQNKSIQAVDYPEIQSQQMQRQASAQSNQNQTRPQSNTIQGNQKLLYMQKQIEEQNQYIQQLSALVGNRKQVFDPEKDDSLAQLLNERDDLQKQHILNEIKQLKQRIDEYDHSRQQLQYGFMPQQQQLLQQQGMMQYQNPYGMYPQMYPPNIQLQQQQQSDESDDNDLNNEILMKLIDQQNRQNKRGSKNKQNSRDNKDRNQKRDRRNTQKKKQQDDDNNNNHGNND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PEG9</accession>
    <name>A0A8S1PEG9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1140009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8100908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PEG9"/>
    <dbReference type="OMA" id="HKANKIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="613"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="645"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="619"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="916" mass="108436" checksum="5504E7C1CC90B48E" modified="2022-10-12" version="1">MSEDDSNSVLEVSAPIKGRLQRAKSTDEEKDEQQKDVFKAEQPDSIEISDQSDIQEVENKKRQKKQNQNSQKPRKPRQKKQNENAANQNEENQEKQNKEKPTKQVKTKKKTVETNEKTIENLLNKKFIQAEEEDEPPRREMEIEYESNRNDRDTFGIDNKQNKRKAQNAKNINQLIKNRHKFFTEFEDTPFEFQNENQNYQEIDTDQFELKFEKQQVEESAIKQKAKRDMLNKVQQYKQNVLVFGDQQKSQKSLLSSTFLPSQDQEQNGNNIPKIPQIIKTASFIQEEQNQRLDGESRNSLLRKTYSMGLKKGLSQEDNEDPELQLFIPKINGAKDKIFEHTLFLNKIKQKGFDRVVQTSTSLNNSNKAYSRSEFQQILQQKQVEHKANKINLSLNELMNRFQSTTFEQIEQRRQQNQQVNNNGQDDDDDSSPDEDFVPKQNELNQESEDIDDGSSQSSQDNKIIDDKVKESKQTEKEIEEQENNEVKKEKEYGIIIEEKSDVMSKIDDEEFQQLQQIKNRKLNQNNDQHQSDADEENENQNENEDEDEEDEEEENKKDDDNNKEDHKVQENKKFNKLAQIDISDDDDQQADEINEDDLNMFRRLKKVSTMFKERKRKEKELEEKKKLKLERQKEREKMKKYIESNLVEDQAEQGSDNESHDDVVKEIKDDDEEEDNEVIEKELLEMIDDKIEDLDEGNEIRAFQIFMDRIHAQEKEDIKKILKGEFKRRNGKVSNIDLINMEQTEEEKQARLMHKFEESEEENYDSDFENYLKMSKNQKKEFLKEKKLQKILQAQKEMNLQTKKNRLQEQQLFQPDLLKVVEKAKKKQQETIQKKMTQKPPSFGQSLQLQSKINENSNAHNSNSMQQINVQKKNIKQQQNNTSYAINRLFSDNLNSKGQQLQKSPNKAVLLMKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGP4</accession>
    <name>A0A8S1PGP4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="2">1.11.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1180024</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGP4"/>
    <dbReference type="OMA" id="NNFGVMR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03015">
        <property type="entry name" value="PRX_Typ2cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000003">
        <property type="entry name" value="Peroxiredoxin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF171">
        <property type="entry name" value="PEROXIREDOXIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 9" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="10">
        <location>
            <begin position="5"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8102287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="208" mass="23332" checksum="7250C82D1272991D" modified="2022-10-12" version="1">MSQKLKIRKPAPAFTATAYVNGSFKKVSLSDFKGKYLVLFFYPLDFTFVCPTEIIAFSDRVEEFRKIGCEVAAVSVDSHFSHMKYCQTPRNQGGLGDMQIPLIADLGKTISADYGVLIDDPQDGDFGVAFRGTFIVDKNGVLRHYSVNDLPVGRNVDETLRLVQAFQFTDEHGEVCPASWKPGKPTMVPDHGSQKLQQYWTEEHAKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIZ3</accession>
    <name>A0A8S1PIZ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1200017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIZ3"/>
    <dbReference type="OMA" id="EDANTKW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="600"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="48"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="300"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="486"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8103070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="774" mass="90889" checksum="16572466B33571C0" modified="2022-10-12" version="1">MSIIENVNKICQTHQFEILAVDLQAAREQKQKYLCVKCLIERIQGNNIVLLSEAIEMIKEMKNQYKDEVEKKNQETLNSIQNLQSSVLKINEHFTQVFDKLQYKINENINSNEQHIEQKKSSFDELNLDKDIETLSLIYKGNSNCESIKQEQNLEEFVQMLDKVQSELSMISNIKQFSQIFESIQQIKSKFQIGQNTPKRRFDKHETPTLKYVCNQHGKKIMMINLSQNEQDQSGLACEKCIQQFPKREYITVEDANTKWKELQGQQKQMISKYNNNRYSKFNSSIKSIQQLKEIYNQTLTDIIQQLERQRNMNQNTQNNNSNVVEDIYELDEQEILKIVDFLSQKDKNYKLKLEQQEQDQVDLIFYQNQKNHLENLMKYNLLTKHNLMRIQNQDQCEQYSITDIIQENQYDSNSNVQSFLKKFLLLEQYCSLFDDSCNFYKNLQQEIDDLQEKGQLSQLFNAEQQQKQYLSFLEYSKKMKLLIMAEENQKLVSSLEQESNQIKQSQQVIQTQLDESNQKIETLNSKIEQLDLQILQYQQDKSQLNESNQSLNNQIEQLKSQITKAQQESTNQLNQKNQEINQLSSEHKSLQQLIRDQPKLITPQFYLEVCKLIEEKSKMKIICSFLIYLGTRDGLNHTKCWSKINGKYNLLIIYKSKSGNIFGAYTPCQWIEKQNGYVADDTLSSFLFSQTHNQFFPLKEANKGNAIYRHQSYGPSFGNGYDIYIGSDFTSGSSALGTAYQIDQYQIQNTSTHLFGQATPDLEECEILELILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJR3</accession>
    <name>A0A8S1PJR3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1200117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJR3"/>
    <dbReference type="OMA" id="ICACYNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="559" mass="66092" checksum="F34B9BAF9DB142BC" modified="2022-10-12" version="1">MDELLSAVMQRKTKINEVLETIQKLKVKNVISQSERSTIYAIIRKLKEEEKNRTEKENWIVSIMRNTKQYNEEQMNHIFVKTNDIMKLINESFIELPYQPPNSLIQFLEQYYNLHQGFFQQMNQEYQLKIQYKQAIKQGNINLITDLIKALLASSRKILKDNIDDLAYQVNKDLSPEKQKIMKKLIPDPLVEHLRTRSSTQNGKKDGTVISMTNSVIQNEEPIRYNDKKEYQQIKEKLKQQQRRFILQPKIPNQEIMLLESPVYQLKQKYQQYNNINIEKNQFASLFNNVNLDDLDDQNICVLDYQTIKRQQQNFEEFKLNFDSLYDKIKMRNSYIQQSVMDYNQYISKKCINSTMMLSSQCNNNLENEFFNVIKDKYEELTSLNAEFQQNMCHYHFCRIYLKPHINPICACYNTNTSKYELNSNPIKNKIIIRKSVQSIKKSTPIQQNQNTFTSKNLIDQLKKERTFNQSIILEDSSYQDIYKYNNSSGISPLNNSILQIRKLDQSQNLNGSTQYKLYSHKLSSDITSFKQTSLHHRAQSANGDQLIIPPLNLRNCAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJR7</accession>
    <name>A0A8S1PJR7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1210004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJR7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="180" mass="21882" checksum="02E245F3EA45D0EE" modified="2022-10-12" version="1">MKEYKLKEANFQVYLCRTSKISIGLKNLYINVSNLGQLIMRKQNNCQIKTNKINQELIQQNQFLSSQVRLIVKVNQQFQLELVYILQKLKYLLKNVFRKILIIIRKQIQDSYERTMKLFILFKFFLMKIKKKIIFLRFLKKIICHLKVISHLQQQISPYTIIHMYNEDKRYFVNSMKPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLK4</accession>
    <name>A0A8S1PLK4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1220020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLK4"/>
    <dbReference type="OMA" id="NQVQSIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="344" mass="40650" checksum="463541AE6D61A608" modified="2022-10-12" version="1">MKNLKECLSSEGSYKNTKQFKENIQNEDTLNYVIETLEDIFQSPKSHPIQQVLAMRVSLLVCLAEQLTKELMDLHYDCIVYSVTNNIIYLIEDIIMIDQKKNERGKFYFSDQPDQSLILLGNTLIRLALESIFVWNLWHPNNLAITQVYQRLIDQGVQFPKLHYFNAQKVKEYYLTVKESSRNGTLYRKQSLMLNEQYFDQLKQTIEKNQYNNIKLHEINEELKQIKHVNENQKQFIENFNKAYKDYLVNNKLEEFNNQVQSICLEYDELSSQKKQNSAIQFCTYSTDKQSNQFSRSISSQNSQQRNGSNNKQQSIEQLLHENELIQAQIQSFEIPKVNSHIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLT1</accession>
    <name>A0A8S1PLT1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1230055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLT1"/>
    <dbReference type="OMA" id="QRKASYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="142" mass="16919" checksum="337F74FAC39833BB" modified="2022-10-12" version="1">MKTNYLRNSFEQQQQPFYQYSKSQIVNQTSKQSFIISENYQSQLLQLSIQPKQNEQRKASYQDEEEKKQQMAQAIQDQIQNNQNRDDSIQKPFVDQYSQPLNEDVQKDQDIKLNTLMTKFFDVEIPNLNNVSIAYLLEELNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZQ6</accession>
    <name>A0A8S1PZQ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1370126</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZQ6"/>
    <dbReference type="OMA" id="TCSLIGW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042512">
        <property type="entry name" value="TLCD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31898:SF1">
        <property type="entry name" value="TLC DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31898">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="43"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8108572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="262" mass="31111" checksum="7ECED1DCC97552E0" modified="2022-10-12" version="1">MYQWDLVNESSDQLRMFSYCFIVWSALFILCYDRIKLNNLSSKKQIDVFNRIVSILHGQFTFWGSLIIILSQTPYQLQEMNSSEMQFVMIVSAGYFAYDVIICTYFDLYDYWLIFHHVISLMAFGESILNKKYGHIIIFGMFITEISNLPMHLRHILGCFGLRQTKIYESIEILYFSLFIIFRGILSPVLLIRTYEDLHAPLLIKISASGLLVYSTYYIIEMVKITQRKIKSYRDRKRRNLTMYWFSQNPLLPSQKYVDSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1B3</accession>
    <name>A0A8S1Q1B3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exportin-1/Importin-beta-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1400096</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1B3"/>
    <dbReference type="OMA" id="QFIVQWA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057942">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF33">
        <property type="entry name" value="IMPORTIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24140">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8109344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="919" mass="107822" checksum="79E2C1EFB1EE7F94" modified="2022-10-12" version="1">MFSTEEFIRQYQKLANPDKTISNQANQYILKLQQSNEAFTIAKELLDQPNIQQEYQFIACQMIYRRLKEECNISIQPYLLELLTRNLPSVAMNQVCSSLAVIIVGNRSLWGGILQELIQLMKAKMTIGIEILTQIAIQGKEWYKKSEQLRLQQEFISQENTLSEIFLSLLMVQDLSIFNQTVQCIECWVQFSYNIFKDIKLTQQILSLIHQTISNNAIEYSDKLFALLNEGVIFSQYNRQQNVNQIDSTVLQNLNLILEFIIKIYNPQLSKGYASFATKFLCDFYAIIFNSKYEQAVFNLMGNIVSNPSRKIAFQTFEFWSQMREYNKLQGFGLNILQTMIDKCRMKTIKLTKEFLLGEPEEEDTDYNINYYEGGQHFISTSDFRENSKDIFYSIYKSAEMNMQSDQYFQIVLNNLIISNPDSQIQIIQSESALFSLCSIIDEADIQGNNQLILQIIHFILSLPVHQNFDIIVKTSLQMFSEFTNQLQLHNEVLLQITQYFFKYMLHPLLGPLAGVAFEQICNLSCLNNVELISDSVKFLELHFSEFINQSQVSNYIEGILKLCYRLYEQGKTECLIQLFNFANHQFQQVKNPQVTEKEFHYNNSLVVTILQWMNLNANQNDQIKQLLELLCQSIFEPLLIVLQGQYSFSLYDDIYKLIRIILKVSNYQNQQQVIHLLQISYQIFHSDPIQKNNWLQLITQAIGRSINHQFIVQWANANDYQIHLFCIESFNRWKDPDLMKVYVEYVKECIRCCQQILFDSPYLQKIIDVICDAFLNLSSYEMQREIFHFFKSLFQSLDNNKEYFNPLVIKIVTTLFMSISDINPAIVFQIIQILLSILQKQVNPNDLEELIFQTLQKSWGKDKQPQKVKLVSQAIISYLLKSKQGEINRNLKLLLENLRPMSEEEINYMQLEIVIKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q3E0</accession>
    <name>A0A8S1Q3E0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1400018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q3E0"/>
    <dbReference type="OMA" id="ESQMFIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="242" mass="28471" checksum="DA565BDBAEEB6BBB" modified="2022-10-12" version="1">MITLHQKSLTESKIKIDNASFQTNLNLRSFNPSKFLNNLDQLHDIQTPQYNENNQQIQPLTEKADSSYCRFPKLQSNSLQNISKPIDEMPQSKHNQLKSQLSNIFFQSNPITENHIILPGLRKQRKESQMFIQMIEQQISFPNQDNQTSRSKRFQRKREQQTQSQTNLDSLNYLLPDNSQLDDSNNYNKKVDFHKRVRVINLENGRIATEIIKEEDESKSPKKSKRKFVSQKTKFFQEDANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4M5</accession>
    <name>A0A8S1Q4M5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1430073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000147">
        <property type="protein sequence ID" value="CAD8110213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q4M5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="577" mass="68403" checksum="3E4990434F6AE516" modified="2022-10-12" version="1">MTQVDLKAIEMVFALLLLSCLTYTWFTFISLKFTIILALTICSLIVLLKIKWIRIKRKGFLNNISDKFRKILLERSLFDILCDIWYFETIKRHLRVFLSPFLIKKSPEEIIESFEDINPKLKEAILRKGTINLFPEPIKKELVENYIQEDELQINQDKQLHQQIIQQPVKVEHWDRYVDYEQYTQKKRKNNSQTLLHVISLLMHKPIVHKENHKINLRKLSSRTKSLLIVSIICIMVQMYFSKSSRRIYQKAFTVLSSMSLLGLASTALGMKENLKTEMSDTTFRREATFSLNYISSSQIFYENRRPSTKKSIFLRTSQNQVRLPHFQTQIIQRNEQCIKTEKNNTIISGKTYITKAMEHQYLRISSENLIAPFGCQIQCTSEFQLMISQIHPFPTNYNCEQVINNRGFIVSVIIGEFIYISIITRKDSELIFNLQFNLQFEDNNQRIKTLQDTPSSDFQQMLKFSHSKSFQCIINSNKEQAGIKKNRYQDEISRSERFLKKLANQEKIITKIINDKKTQKVIFEQQWAIIIYFIKMSQRIFDVFSHKNISNQITFKINEKKNNMSKRRIFRNKSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q588</accession>
    <name>A0A8S1Q588_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1420070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000146">
        <property type="protein sequence ID" value="CAD8109951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q588"/>
    <dbReference type="OMA" id="CITNQVN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="388" mass="45405" checksum="685391470460AB14" modified="2022-10-12" version="1">MGQNNCTPNIPGLENCYARYEQTDENKYPYLVANKHRLAESQNSQIFELYQKQQDQLRCYLQNSRQLSQSTRRNYEFEGRFLKLPSHLICHIMYFVIDDYINLLLVNTLWYTRLNEAFSKTVSIVDEKFQQSHSFFKLKQSKNAFQTFQFGRQQGFRLDRCLIAEITPGVENSIVKISYTYICKGQEKAVCYKFGILRKNTIRTIWIGVDQSTNIYDKQTVPVIQPVLPFCVGDYIKIPFTLLNLQGQVNLQSIKWNKPQIEKLDVGIFGNPKHITMQPPLRFEPKDADWTLFQYFDHPMKKCFDNFKQIKLRECRCCGNGLVINRMNYTCISEGLLMIPQLNMKIIVKQGCITNQVNRATVSYELDNTLELRIGDLLTLYYLRGGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q6D0</accession>
    <name>A0A8S1Q6D0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic peptide chain release factor subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1460060</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterodimer of two subunits, one of which binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8111028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q6D0"/>
    <dbReference type="OMA" id="RCNGSEE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000032">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000003">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.960.10:FF:000003">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="7">
        <location>
            <begin position="7"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8111028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="437" mass="49417" checksum="B7AC58F481D5140A" modified="2022-10-12" version="1">MDSKLSDAEIALEQFRLKKLIKTLSQERTAGTSVVSVYIPPKRIISDITNRLNTQYAEAASIKDKANRTSVQEAIQAAILRLRPYNKAPNNGLVVFCGIVQQADGKGEKKISVVIEPYRPLDLSLYFCDPQFHVEELRALLNIDPPFGFIIMDGNGSLFATIQGNSKQIIKSFDVDLPKKHNKGGQSSVRFARLRMEKRHNYLRKVCETATTCFISEDKPNVKGLVLAGSADFKNDLAGSQFFDKRLQPLIISVVDINYGGEQGLNQAVQLSQESLLEVKYIREKNLVGQFFENIDKDTGLVVYGVQDTMRAVESQTIKTLVCVDTLQYLRLECQSKQTEQKAIKYVKGNEGYEAGSLIEEKNGEQFVILLKEDLVEHLSEKFKDYGLDFQLITDHSVEGNQFMKGFSGLGGFLRFKMDMDYLVQQEDWKDEDEDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8M2</accession>
    <name>A0A8S1Q8M2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1490095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8M2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="134" mass="16463" checksum="DE5B18637DA6D319" modified="2022-10-12" version="1">MKMEFLIKEEAKYKVYQKPSLKIKQQYVQLSGTVRINQQFSIPQKYQKQSILQQYIYYQNYCQQNEKMKFYNINQCIYKICSIISVCFHQELEIFKKKLLIFLKISVQPKHINKLYHIMKLMNFIQQIHNKRPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFF7</accession>
    <name>A0A8S1QFF7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1550094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFF7"/>
    <dbReference type="OMA" id="ETIIIDM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="156" mass="18019" checksum="8F56DF1BA1C323CE" modified="2022-10-12" version="1">MSIYIIDIRSLNFDFINLKSISQSYQKIMGCCQDRPNNILRANELRPLTKAPSLILTQRAQVMEPMKISIIGDIDKMDFMDDFPQPMPSITQIDSKFAAKQISSRELKETRDLKLTQLSETIIIDMRSGTFLQSPLKPMSERRSSKNLNQKFIEML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPP2</accession>
    <name>A0A8S1QPP2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1810019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000190">
        <property type="protein sequence ID" value="CAD8116805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPP2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="80" mass="9626" checksum="5A0A90410828C4BD" modified="2022-10-12" version="1">MIILIFWSLESSKQNHIYKSNGFHIMQGQTQQNFICIYLEKIYEKLIKKYLIFGVIQEKLRRKIKVRNDISSNLFLCSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPS2</accession>
    <name>A0A8S1QPS2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1960002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000205">
        <property type="protein sequence ID" value="CAD8117341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPS2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="124" mass="14763" checksum="2D7E0A50EC7A42AE" modified="2022-10-12" version="1">MVEVVNISGQKSQIIPKGEIIILENYQEFYLNSRTKDPQGLLLDQKKHQPALCYFRQKNYQNWIVMSKKREPDQPDGLDGQDLQTRQRKVQFTYKREINDLNRQNDQQIEKQVRQLKEVLTAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPI2</accession>
    <name>A0A8S1JPI2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WDHD1 first WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0040574</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057646">
        <property type="entry name" value="WD40_WDHD1_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24817">
        <property type="entry name" value="WD40_WDHD1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="WDHD1 first WD40" evidence="2">
        <location>
            <begin position="269"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="303"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="345"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="387"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="429"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="453" mass="50542" checksum="3BB2EF06003A3012" modified="2022-10-12" version="1" fragment="single">MQAHFQEKEALADIFGHIKDVDEQIFGAILEKLRREKVQDIIGYLSDNRNQSQLESCTLYKGVDITQADKEQKLSVVRNDIKKITEVLRKLKDHGFNKVDYSSEVNDESTQSLINSIKDYRWIFEFLYFLVHLTSIDETLIQCGSNSLHLLVQMKVNLRKKNFENINLQNTSLVGANFFKCNFSGSQFYNVNISGINLNRALLINCRWKELRINELNKLNGHTGFVQSVCFSPDGNTLASGSSDNSIRLWDIKTGQQKAKLDIHSTMAWSICFSPDGNTLASGSVDKTIRLWDVKTGQQKAKLDGHTTEILSVCFSPDGNTLASGSNDKSIHLWDVKTGQLKASLDCQCNQVWSVCFSPDGNTLASGSSDWTIRLWDVNTGQQKAKLDCHYDAVKSVCFSPDGNTLASGSQDKSILLWDVQTRQLKASLDGHSNQVCSVCFSPDGNTLASGSX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPY6</accession>
    <name>A0A8S1JPY6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0060047</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8043965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPY6"/>
    <dbReference type="OMA" id="PQTQMIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="225" mass="26427" checksum="0B3473F44D705A07" modified="2022-10-12" version="1">MSFPNQDYLAMLKAKPQLYPQMQQTPQTQMIYPNPQFMQPYMNCVPQYFMMQQLQQMQQMQQIPTMASMGPLGMPLLPSQPHMQPLPQMVMPFPIQPPIPAQTKTDQNLPVQVKGTKVKLNLKMIKDIPQEEDSVLDYDEIKKQSGDRQFINLKRKRRLLDQQDAPLMQTRRRSEQSQYLEIREMLNVDDKDMTLIMEAYPDTKSLLQELKNGNLKQEIEDLLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQ46</accession>
    <name>A0A8S1JQ46_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQ46"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="36194" checksum="5951F1C833B06D2D" modified="2022-10-12" version="1">MRRPNEVEYSKTALMANWYEDRLAPQQLQRDPNAQKSLRTVEEGISIPGDNGCLLPLPRVNRNPPYITKEIIVPDDGYREFRTEFQTKFDAKNIQNMQLGDCRALVKTNGEKVNFPECNPTVAQTAGARSIKQLDQTNMQQILVDRAVHQNYTDFGSTFRNHPEQHNKFYSITTYQQSFQLPENNVKEILTDKKVKPIAAGCRERDPFDQGLKMTSVITGEKYRDSVDPKENTEVQRQWVHCGDSGLKTAQNNLRSSSIQNYKKTAYQVHPYDIATSLPLGDGVYTLHPKYTDPGQFRHIRSDVTRIMNKPITRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRS6</accession>
    <name>A0A8S1JRS6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRS6"/>
    <dbReference type="OMA" id="YPNNNTG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="163" mass="19132" checksum="DDC93EAC6A3C62C7" modified="2022-10-12" version="1">MFIINKTPNTITLSQPDLQEYQDFFNHEDPRKNQSVTIYDRIKKVKSNQNQNLQLSSKLSNLSLTKTTFNNINTLYQFTKKTLKQINQKQEITLSQNFVTSMKESNLFIYNVAQKISTFIKTDKVTTTSQDYWELGVIIEIQMLKKEIPLKFTVSKSQHWIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JT69</accession>
    <name>A0A8S1JT69_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JT69"/>
    <dbReference type="OMA" id="PYSNDEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="362" mass="42928" checksum="83223DF324400502" modified="2022-10-12" version="1">MNFFWCCEVDNQTISDRTSQLYFSNNAYSPYKILEDMQDPKLENLQLTLYGEENYSLVRNTQLNVISQDNSSPNSLPIEVLQMIVQKSKNIGERLQVFIPQSNDVQLLKYFIQQPKFKIYTLEDNQEYLNILKQQKIFKQLQYISQDFDNSFDLIIFNCSSSQERISSFFQNYQMNSFKNKVFILDYCKNIKNIISQIMKDQETQKEELMTISINGYFRYYIFFIGKISSTQNFSRLKSIEAILLQDYESQTNYQTLIKQMLKYLKQQNIPYSEILKIIGKIQSENHDELKKQQSDTMLQRFLSKIIANKNIDKSQIFEIFQSVFRAGQFTITPSQEQFSNNQINSYKCAILQNNSRMNDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JV36</accession>
    <name>A0A8S1JV36_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0110070</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JV36"/>
    <dbReference type="OMA" id="EIYIIVF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8046724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="18469" checksum="F77C547DAACE0D72" modified="2022-10-12" version="1">MDQDSDNSEIYIIVFVIIFLVFMGIALFIYIRKKQQNRNKKLEEEINKLQQYVNSCDRKQESRTDNVNTQPQILSQTNSFTKTNRMFQQEQAEQNKQTGKMFLDYEKSEREIINQHSPHSQKSPPDEEEKKQTIDSGRKKVVGIKKKNLQIEIPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVY1</accession>
    <name>A0A8S1JVY1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVY1"/>
    <dbReference type="OMA" id="RDQSCLL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="73"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="247" mass="28490" checksum="C89153C75276FDD6" modified="2022-10-12" version="1">MYQKFETTPFSSFRQQYFNNLREQSCLLPALEELCGGWTQETLKSILQKLTKKNQAPLPLFFDSSQAMDSISNKKQALATVFQQFDSRGIGRIDATELFSVMLLLSTGEISQIFYNIAVIFGSDKTNHITSDEFFFFIDCLFRGISKVLICKGETKPINLNKRLNDQDINKFIQQIFKGQQKVNKDELYASVKQSQQLFEFIEYISTSMQASMEYTRQQSLLMMKITMEVKKLMAQMLSQIDGTAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JW58</accession>
    <name>A0A8S1JW58_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0110144</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JW58"/>
    <dbReference type="OMA" id="IQKNHFR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018256">
        <property type="entry name" value="Ribosomal_eL13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01104">
        <property type="entry name" value="RIBOSOMAL_L13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8046872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="208" mass="24300" checksum="8E3425F9B5EBF0C3" modified="2022-10-12" version="1">MKHNQQLPNAHLRKHWTRFVKTFYNQPAAKRRRQLRRRTLALSSSPRPIELLRPVVRGQTIKYNNVQKLGRGFSLIELKEAGLNASFARTIGISVDHRRRNLNQEELNNNVKRLKAYLSKLVLFPRVTGKPKNGVVKDSTNEVVAQPVAQNTNPEVITFQRTPKREKATVISKELRAKNVYRRLRQEWYNAKFVGVKEKRKQAKETKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2R4</accession>
    <name>A0A8S1K2R4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110289</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2R4"/>
    <dbReference type="OMA" id="PHTMQKK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="143"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="257" mass="30589" checksum="62BB985FDCA0E88E" modified="2022-10-12" version="1">MQTWDSELPHTMQKKLSLLLEQRENLQQQESQLKQKNLLFAHKKSLSKLQNQNQQTVETLLENEKIELIDIFDQELINQNSRRYSSRRMPTLEQSAISFQEQSAVFHKSNKTNRSASTNLSYFNTQSPQRLSKFKIDTNTSRIQDQDQTIKQLQSIVAEQQEKSLIQNQEIQILKEKNQSLIRQLEISEKNFIKFQKQTDLFKENLVEKLKTYAQKLEALNENEEKIMMMQKENARLKQEKEELLFLMRNNNIGVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K331</accession>
    <name>A0A8S1K331_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Steroid dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0140125</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K331"/>
    <dbReference type="OMA" id="NINMMAV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051019">
        <property type="entry name" value="VLCFA-Steroid_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899:SF13">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8049709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="33249" checksum="94ED803BD3BE8B41" modified="2022-10-12" version="1">MFFYIIGLITIAFVVFRILEEIYKCLQPFPNIQAKYGKDCWAVVTGATDGIGKGYCQVLAQQNVNICMIIRNEEKAKKLIQELSNGSTSKFKIVVVDFNKSLEEGFFDKIYKQIETLDIGLLINNVGVSHQAPLEKYTDNQLKEIITVNCFPIVFLTKKIITNMLKRKRSAIINLSSFTGRVPMPYNQTYAASKAFDDFFSRSIALEYSNIDILAHRPMYVTTPMTNFKKGQGAISPFQAAKGALQRLGLEYSTHGHIFHRIQGFFGAVVVPQFIRNIILKKEMRKLVRKHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAU7</accession>
    <name>A0A8S1KAU7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAU7"/>
    <dbReference type="OMA" id="LILEPCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="214" mass="25030" checksum="D694923642BCC946" modified="2022-10-12" version="1">MGNTCLHRFDNQKQKVIKINGYPKLNCPNKLILEPSIQNITQNLDTFEEDFFKSEFQFDIQSDNQKNYSPSRVKLGSSIEQINKSEQIECPSHFNEIYDFQQDNQLKQFMNPNNNNKNVFIQGQKTNHQKKIKQKVSLQKIEKPKSILKTHKKQHNSSFNSFQCTKCQHTVTFSLATCNSRNSRSLSPLLNSLQVDFQPVNSRSMTYLQQFPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJF6</accession>
    <name>A0A8S1KJF6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJF6"/>
    <dbReference type="OMA" id="EQKKFNW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="301" mass="35377" checksum="AC35A2BB29899B8B" modified="2022-10-12" version="1">MTIEQMYSVVRGETGAWGIEGYEVPKKYCDPLKQVQEREFFAGKLKKPPTITKRGHFLDDIAKPFRNRKVPVQYNVEYKWVNEKDKERKRSKSELLKRNTFIDDIFLQNSKQNYPKPGPGKYDENRSIENQAKKWNGKTKIQYGEKPDFVQDYQHLGAVLPAGPGSYNPHPILPKLKHNNTNPKIMIAKHKDFDKFKLEMMKNYPKPDCTKYKPYPVDYNTFGRSLELLYDKKKIEPAKIKYWGNPNKPVTRNKSEAKIPPQYNLIAYWPGKDDGSAKNKELKKFNWMNKVTKGIQQSIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KK22</accession>
    <name>A0A8S1KK22_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0220169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KK22"/>
    <dbReference type="OMA" id="MSIWSKR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="81" mass="9081" checksum="80991433AEA9763C" modified="2022-10-12" version="1">MDKQQKSVQNSQMSIWSKRTSIETIQNEALSSEFESSTQDISKYNFVIGILKPGQKRIGLIAINKNKLSIAGILGIKKIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLR9</accession>
    <name>A0A8S1KLR9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLR9"/>
    <dbReference type="OMA" id="MYILNQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="56"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="279"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="525" mass="64006" checksum="DAA2B84D10A2B49E" modified="2022-10-12" version="1">MYPKKINRTLHIGRQIQKDNSIKSLIIDKLIQDYGKKNQLNINLINTYVEDFFTFYKASEGTVRALKEEIQEAILRSENEKQLMIQKLHQRISRKQKEEQLKILTNPKCLVTLPDKNDEQNNFYKMQNKTDNNSKIQLINTLPNDLLEERRKSIYQVDGSVDEWAAIIKYDTLQFKKELEEQKKLMYFNQQKIKEELDRQVAEKEQRKYKEKQEDKEYQAKNQQQLMEFERQQELIKENKKQQLLQEKFIRDQQVQIDKRKKKEQNLNTRLQEEEMLTKIKEQLKIEDQQQKRKRLEQEDKFQKILKQNENLRKKAQDEITYSQENAKLLQEQQLIQDQFENDRKDKYQKDQEDQEQKINQLMNNYSQFVVKNKKDHYFQDDYWTKKNSEHNTFFDFKNKTNKKEQQMQISYDLQQQILEKKRRYTQEVNEKKILFEQQLQEQKALKKQDEERRNNIRQMYILNQEELKKQMVKGYLSQSPKKKTSPSERMVGYELLQNKKLLKKIANDNSIGISIKKQEISIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KMG2</accession>
    <name>A0A8S1KMG2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0220306</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KMG2"/>
    <dbReference type="OMA" id="MRQMQQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="115"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8054965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="318" mass="37337" checksum="B5A4CE80C332E3AC" modified="2022-10-12" version="1">MNQTPSMRQMQQYGQQGYPQQLQQYGSPKAAIPSFQQTTQNQQIQTQQSRQGNRADVHHFERPLQVVPQEDIEQKWKEKCLRLEMHVYELQVEIQKLRLQNSSGQITYIQDDTRINELMQANREIRNQEQQMRIQNKQLLDDLESWKSRYKLLQESNSKSSGLDEEIRQLKKKINELTEDLAGSHEQIQQRDNEILNLKQLLHDKDNELDQLDARIRELEMMCENYSQSETQIISLQGEVELWKKKFKQVNEQNSDLAEKLTMAETQLEAIKKRQVTVTKETEVRKSGTHGGGTTSTYEQNVIKGAQLLHPRGSQYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRJ2</accession>
    <name>A0A8S1KRJ2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRJ2"/>
    <dbReference type="OMA" id="HIISEKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="23808" checksum="0CA7F31CADC628B4" modified="2022-10-12" version="1">MKLRSISTIDHPKEFLFPIEPDLSASMTVTNSKIYQIYQKNRQTTPKRDPLQSQFNFTNTFRFENNGQISPLRLYPEKAELINLSSKRSLTPSSKPQQIKVKGFTDKFNEIQLKKTLRHQGVGIVYCRQNYNKINNKVDGTGEIMLEDIQNLNNVKQRLKTFGLELENEGIHHGVKHIISEKFARILQTEKNKTSRNQHNYFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTK0</accession>
    <name>A0A8S1KTK0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0210384</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTK0"/>
    <dbReference type="OMA" id="LIMECEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF6">
        <property type="entry name" value="DYNACTIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="9">
        <location>
            <begin position="28"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="84"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="201"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="861"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8054274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1097" mass="128070" checksum="847F7E6CCFC32B38" modified="2022-10-12" version="1">MEQLQEGDYVTILKDPIRNEKGYIKYIGELEGRSGTYYGIQLENKLGSHNGTLQGKEYFTCPDGFGLFITGNHLKKTIITRDANKKSTKVTKDEPKQQKLKTSASSAAVVQKSQPQSQAQYGSNQTTKQTTLKRNESKKETIVKKHIEEQIEERLSTLEAEEIQEPQQLVKQQSEPEPQFLDQQQELESVKQQYHALKIVANDYKLENKSLKKKVEQLEQELTEQLEDLDSYEKLIMECEQLKSDKDILNLKLQEFEASNQQLQQQVSQLQFEKELADLEIEEALVTCGANLQVGNQDSSEVVLKNTQLQKALAQLKQEYDDYKQQKITEIQQYQIRLEAIPQLNEKAMKCVKLESQVKKLTEENQELCQRVDESQALQEMIEKMTETLMKKDDFIEELRNQIKTINEEKKLDQELIQTLLDESNSLEKQINDMQFKLDQANAQINNNSNDLQEKDQQIQRLKVKVAQLNKQVDQSRLEEDNTQNEHTKKTFLDEINLKNQELLLQQRDTYKKYMKVALNNIEIRNQIALKGIIIYKSLPQDYCTKLKFNLLEPIILVRKLSEKSILVADEFLTQLENQSDKFIKEELQDLGFWMLNAHKSLYQLSDLCNIFTIYLQNQTDRESLEKVARNGILLNQFKIADTLVDMIIQCVQNEKISTQLSLSELQFAIQRINDDLSNLEIQEGAVIESLLNQIIFKYSYSIFNFKVIINQSTATTLQKITVDQLIDTLIQRCKELSKQLRYGRQCWGGYFNRKNEKFSELINYLQQLLQQDLNKLELDQYQSYISCQGESVSQPYRDRCVSIVHLLNQINQQFEEQNINEEFLSNKPTLHSYAQTSLWSQWQKELKSQLSDISYTIESEKRLKDDLQKLQSEKSQIYSDCVALRKNKEALENRVVDLQIKADRVQVLENEKQRHLEKIKGFNEASELTRKEIDNLENKLKESEEIRKQLEEQVTLYQQSQPKNSFLDQLQGRNFRKSGTMLIQSSNNLDSESNFNPFQEFTINKGIEAVLLKENQQLKLYKMREDILSITASNQKTQIEEKLLQIRKLKMKQLKSMVLNEKFDARNQAAKFYVKQSGMKLTNTVLMDTLQAIIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUR0</accession>
    <name>A0A8S1KUR0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0270065</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUR0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000038">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000099">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1420.10:FF:000043">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF153">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="3">
        <location>
            <begin position="1089"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="389"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="489"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="906"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="424"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="497"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8058141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1248" mass="147375" checksum="3627B75ABD2A7500" modified="2022-10-12" version="1">MKFDQEKTDEIKLNPRYQNLLKWGLDNGVIIKDVDMPAAFGELTGVVATKDIPANTAIICVPQPLIISQEKCKLSSLSIVYDKHPELFDENETSDAEFNILIFYLFNEKKKGEKSFYHPYVQAIQSNNTLIDWTKEELNYIEDPIILDEFAIVREDLKELWNQAKDIFNEFIQIFGETRPTDKEDFYWAAQSVMSRCFGWSLKSTSMIPIADFLNHSNKACTHYLVHSKIEKLEQEKLQAKKDGGNEQNNDSDEDSFMQIVHDQYKLKGNKINLNVLNIKQDESQLKKFIDPKNTYILQHQQYLTENQLKDIDNLDNISNADKRAMINWINYEQMIQNSEVNLWDLGFVTSSDSEDNDSDEDVEVARNKQFETLKIKELSDWKLKFEEKKQQKNQKKEKNEQEIKKEQQIEQSIEQPIVQQNQEVDKEQSDGELKSQTDNESILTICLNNEKKNLVTIKGLPPQQIQALKQRQQQMMEVQQQKQQIQSFIQEQKQQQNKDNESDSSEESKWDWLEENDKDVYFCITTTEPIKKHEQVTVSYGRRTNRFLLSWYGFTLPENKYSSFNFRLWLNTEICKEKILSQKQIFDTITINKLISPEEWDSGKIKFNGNEIPISSITKEFRIKKNKLNMDLLMYLRLYLMLYQVQIKDVLITIPVSVDYEVFVMQFCIQLLQHYLNSYSQELAQDIKELESQISFSRRFALHINKERKEILINQIMILLDAIIILKKYKESNDLKQSYISEIHNNVYYKMEILRGLKVNWSSLLYYLIKMVSIRLKDIALPIKFNLQIGANMIGNTQEHEICINNKQIVNNHCCVILNEDEIELIVLDGPVFYKNEDGQFEQLAKDSILSLNIECADFSIRLGQCIEAKVQVVNGTQHHYLPRLQPQKEIKQFNLQERLSTIEVKIVESLYTSQQSELEQLQQNQEQEQNNIDEINSISIIQQQTNNLEQQQQNIQKSNFEQIKQNEINQKTDILQLNDYEQQTEMLSNGTPDFNNNQKDKKKIQNNTSNLDYLIIPQRKDRESSPKKKSNHKLMKEIKKESYRMSQRKCNRWKFCIAFSGFHPSRDEQQFLLLQNIQICLEDYNFNMLIMEDNIQLRSIKLLIALAKGIPIISRNWLTRSINQYEILDHNQYYVQFSNEFCKEYNFDFKQYQKRLQKCKEAKILPLKGVTIYVPKRMIYHIEHFELEYLVESLGGKLVHQIHDDGEAQIYMLIPKDQTKIIGYDKYQQSPIECLFKSALKFKNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVP4</accession>
    <name>A0A8S1KVP4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0250044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVP4"/>
    <dbReference type="OMA" id="YQKFERR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="112"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="328"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="417"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="773" mass="90839" checksum="5A1EDA58E6185335" modified="2022-10-12" version="1">MLQSPKNAISTAKFQQKVMNLETNTSQFDATSTIIQSMYLQIDELIETIKKRNPKQIKQQKTEDNSLAYQNQLNMFRADEESTNLQQYYLAIESEEISNALYAKPNDVLLQQAQMKKTIQALQIKINQTKQTLSVTDNEYRELESKAQAIQEKQQNYASILLKLKEKQQLNDSVNKLLDQVGQQQWLKLRQLFDVDMIKLSIPFEKLIKLQQLLLKESENLIQVGKHQCILEEKKQFLIEIQLQSPLINLVLSFDQILLNIIHQPKDMLISKQGLLVQTVKCLNILHQNFCCIIQNLTKLNYSQKYFEESQKELIQVTAQAKTFSLNKTQLLIEIDKMKKQLKDLQQQLQNSQMTTDRNIENEVQNLLTMYLKDKEILMREIKFKYGKQYHDHMIMDVKQEFKKICLGQQFEQTKKIEQAYNRMDEIQKQIIKNKDKIIELLKQLKEHKINLQQTKTEESQIDKKEFLLQLQNLKIILLQLENKIQKKTQHNSEQLLQDLQVEINKLKEFIDQNFFVQKQRSDSIDTIKKTQKFEHARSQSQIPIVSTLGSEISGPIQSPKNIQYPIQNPQEIFDSSDDPIQIVKKPFSEWNSYQLSFNQNQLKSISGISGQEQSFITQKESSLHGSILEAMSSNRNKKPEIHLIVFLKLNECSKTYDPTKNEDPQKYGYRLYRIDNQFNIFSSEENENMNSYQKFERRLDVKQLILDDCSQNALKFRMIYEPSMRGIVQDSLALRHLNMQHLPFKLQGNSVIVNGLCLNEQDLIKLQLLFKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KX41</accession>
    <name>A0A8S1KX41_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0230239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KX41"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="175" mass="21174" checksum="4D1BA22F192A9792" modified="2022-10-12" version="1">MYYPSSTTYTTQSYRKFKKQEDRVENTNAIQDYHLQRTLKQVEQLQRDLEQAKMEEQNSQKQRRQAEEKLYHFQLKYDDMNKECMNLREKIAKAEGDRQVIRAQGEGEVKALNVQINYLEKQISELKLENEKLKRENEQLHYQVLDAKNLSKKPIQNIPQNTQQQQQQQRPQSYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KY15</accession>
    <name>A0A8S1KY15_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KY15"/>
    <dbReference type="OMA" id="ALDASWC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="222"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="333" mass="40420" checksum="1B7A974D56A751A4" modified="2022-10-12" version="1">MALQQSQRYDADENLSKFLNKELGKSIKFQKKHEKELNNLLAYQQILQDEKLKNENKLHQMFYKEKLIDHQKKQKEDFLKEQKVNKDHQYLYKQKENEYINALDASWCFYQDYSPSKDMKPLKVDAEREKVLGFLYKQLQTTNDNVEKALIKKKINKFRDSEISKKKLDQIDHKIEYAKQVRSESLDRSIDKIRIHDIHIIDVQEKSNILNQEKQSYYIDKINQIEQKIQKIEKQQQRNKLNRLNIEQQKDEYRQAVRSNSQTLMHQKELLNQNHFSEKIYKLNFLVDQKQKLLKQRQYLLDSLNQKRVIARKSLDDVKYPQLNSNNYKAIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0S5</accession>
    <name>A0A8S1L0S5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0300141</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L0S5"/>
    <dbReference type="OMA" id="EETLTQW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026306">
        <property type="entry name" value="RSBN1/Dpy-2/CEP530"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354:SF11">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354">
        <property type="entry name" value="ROUND SPERMATID BASIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035850573" evidence="2">
        <location>
            <begin position="16"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="853"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="856"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="880"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="894"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="912"/>
            <end position="943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8060451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1030" mass="116303" checksum="18CED0097B692C1F" modified="2022-10-12" version="1">MRIYALFVLLMYATALNFEASLMSHTLAEVDALDTSQLNCQTPRSHFEDLEETLTQWQQVLENKDRIPSHIETLTQMKHLIKHKKYEQLVEKIKDLDLISPPVLSELQSKLQGLQSHDRHEREECTETLTKLCAYLIKQMNNVHQQCQQSPVTIIKVKGKIDDLKIIQSGCQQACQPTCPDEPVPEVPSEVASEEFTEEPVEEGVPPTTPDEEPTPDQPTPDEPAPEDKPTPDVPQDEEEGPTDEPPVNPSSEESPEEPTIPEDGTEEWTIPGEEPEEPKSVPTTPDSPEEDQEETPLPPVSPDPLEFPEEDEEQFIFEETPLPPVSPDPLVFPEEQEEFFFEETSLPPVSPDPLVFPEEQEEQFIFEETLPPVSPDPQVFSEEDEEEFFFEETPLPPVSPDPLVFPEEQEEQFIFEETLPPVDVESPIVCHSSESTEKIELTATQVEPGYTSTLTITNEYGFGLYFQKLQKYPAFADGEEFHLASLQDNGNTVLGVFVTKTGVKCVTADGSTQQSTSVQNDDFEGDWSFVFISHGKGVTGCGVKFFEEQPALRQTPASHPSFSTLEFIIGGEQDSHPSFQGQVYGHFAVSAPNLHFQDEKTYKQIVDSCNNPPEEVCELDTHELGDFEFSGFDDSSEERYDFSTWDSGYSVSGWFKWESLSDQDVWHTVFRLSENTNANLDDIQLGDRDLALYLGNELVGNRYQVTTYTYSGSGNPNLWDSVEYGSTLGQWHYIYFGYSRRTRYANYAIYLSESEMPSNQIHDVRHYLVPQRRLYIGNDGLYPAFNGKFFNWRVNSCIGDVAIDVVTPPKDIIFGYTPKPTGDEPLPEPEVFSEEEEPFWQEEEEEFVPIQPEDIPSEEESVWPEEEEEFIPPPPTLIPEEESIWPEEEEEFVPPPPPVTPPVIPDEVQPEPLPEPEPQPQPEPEPAPAPKPAPGPAPPSEPQVPVVKECEATIDVTKENAADILCAISEYLAQLASGHVPELGINPSRVCFCLQYDDSESNESFMQVEAILKENDAFTINKLVGQRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L252</accession>
    <name>A0A8S1L252_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L252"/>
    <dbReference type="OMA" id="NIKFCLF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026749">
        <property type="entry name" value="Tmem135"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="20957" checksum="3A755118C6AD02E7" modified="2022-10-12" version="1">MNLRIDRFIQTLLAKSQHCQHGCSCFEHVSIQSLQTFKYAYILRTALSLLQILVKFKKNKLKQILKILISKDNIKFCLFLSSYTLFTKGLHCLLRNQYEQDSPKYAFYCGLLGGMISLSFLKYEQRKFWALYMIVRALDTRFHNLQKYYLIIQLKQVCLCYHLFIYATHCSLCLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2I6</accession>
    <name>A0A8S1L2I6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0280062</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2I6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050836">
        <property type="entry name" value="SDS22/Internalin_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="4"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="386"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="514"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8058876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="566" mass="66331" checksum="94256F425E8A4311" modified="2022-10-12" version="1">MNTLECPHGCGRTFDDNKKMDDHIKRRHFLQQRVYPSLYENTNVNMMQKKVNVIRQKLPQLHDNHLQTQQSSTITQQQISESIIQQLPPKPISKIQLQFKKNQILRPNSSNRKLEDIQKIGDQKNKDYNDNKEIQKNEKIIEPSPVISAQDLVEARKIITKQFICTNSDGSDLESVTHLSLIDKNLLIFESTTDVQFQELIALKSLSLSQNKLINVIGISVLHNIVDLNINNNKITTLQPLGECKRLQKLYANHNLINTIDIGLMIHLKIFQIGNNQITDFEHLVQSIKLMPSLKDLVIEYNPCTQKYAYKYDILWTVFLDKLDNQIITQQDYNLAQAFKDSKQESKLSQSMNFQKGLRPQTASTMNNNTFQQDIQNDNSQSNIMLEQLNYELEEEIEELYEINSNNREKIDKLEMQNVEYRLIADRVPFLEEQLQDMRSKISAYQTLIIFKDSPDHELRKHIFALTQELERLKSNNNTVENYEEENQTKFNMNQSKFQNTETTFIQQLSQAESDNENKEEDEDDDDKYFRDDENISQIPGQEIMELIMRNSMTLSKLKNEFKDIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2V4</accession>
    <name>A0A8S1L2V4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0250181</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2V4"/>
    <dbReference type="OMA" id="FYLWFFL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="433"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="562"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="596"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="663"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="799"/>
            <end position="819"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8057004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="863" mass="99917" checksum="B756BD2401E3B0FB" modified="2022-10-12" version="1">MNFFRSQTMGYYKLIIPRESAWNVMNELAELDCIHFVDYDPTLPMINRPFANYIKRCDDLLIKLQLIEHEMKKYHKKITYCKDVNFLIQNFKQLIKERSKASHTYLDEIENDIDKKHQQLIEQSTNMENLHERRNKLIEHKSVLLKGEALLGQSFFQPANYVAEGYVNLQGKELDDIKVLQGQVKFNYLVGVINKEDQIRFKRIIFRITKGNAWMNTMDIESDQIVDTKNDDAKVIKSVFVVVYPGGGGSNVITNKLNKICESFQVAKYTFPENNMVFQEKLRSIETELVETRNLLEMTKNQVEAYLDDFQRVYQNSNCSQIEELKLFLVKEKYLYTQLNYLRVQGSVLYGSIWLPQGADIKVDQALREVQTNYEGLPTGQLQISPPEGTKPPPTFFETNEITWGFQEIVNTYGMPRYKEINPGLFTVMTFPFLFGVMFADIGHGFCLLLLGIYLCVYNREIKESDSLMKHALIVRHMVLMMGFWAFYNGWIYNDFMSVPINLFGSCYTQEQIQQDEQDIQDKQDEYYWKQKQNCVYPFGIDPVWMCVPNELTFMNSYKMKLAVIIGVIHMTFGIILKGINALYFKNWIDFIFEFIPQLIFFTCTFGWMDFLIIYKWFVDWTGNTDKAPSVITLMINMILSPGKPVNPPLWGDGQSEASTQTAMLLIALFCIPIILLPKPLILNSQNKKRYALNASNLTENLNKDLYQKINEDSEGTQENSEIHTASSGGEHHEEFGDIFVHQVIETIEFVLGSISNTASYLRLWALSLAHGQLAEVFFQMCLNGGISSGGFVGAIRLLIGYSIFSMATFGVLMMMDVMECFLHALRLHWVEFQNKFFKADGYAFEKCSYAKVMQDNAVPKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3M2</accession>
    <name>A0A8S1L3M2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0330274</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HYDIRIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="363"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1020"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1331"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1020"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1047"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1069"/>
            <end position="1079"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8062670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2083" mass="248867" checksum="BF426834AEFC4F7B" modified="2022-10-12" version="1">MNEITEFTILQLKSQLESSLENTGSQSKESLKLLLQYSRMLSSYAEQQTNNKAITILNQAFKMLLPDKPPKLLSIQFQYWRLLIMTAIKLCKALEINGQLQQGLNVLIQIENITKQYNKSLEWAQNVELEYLYYHLAIIWEKLQNKERTFYAANECILLLKSNLKNKAEQKQIHMIAEMLKLQSQTKNSIHQQLSYFNQIQFYQSKYLGDNHKETIKTIMRINQLKSEIQFEKEIEEFSKEDFKKKIPFQEVSQIDFYGEEKSFNLEQQQQQQQQQQTKQFDSKEYLRQRLFNQQKQLVIHAKNNSQSKSSLRISHQSKKSLEDFKRFTDTKINLKINSRTSSSGDNKFYYFLNVNNLQGRTKTETTQYRTTQSKNQDRLTPQGIPSQQFRCKTNKSNVSLNNTQNQQELNSGKNVKDDVFKDLMTQRPKSSIAQQRLAKQLQMTIGHQVIVNKKSSQQIQLSNSKPVERMLKKLNTQELNLKKRVLNLQQPIQHSEVSTKKSNQAQEIVFGPQSQKSINLVTSGDDHTQKRSDLDQFEYNLEKIIKIQRFFRFKLKQFHNYDIKHEKINLNKFSVGFVHEDEDKFADKTPALRGKSNNFKFAYQRSNTSSVESIKFASKRDNFLQPQLFQYKQKDHSFNSQASSKLINQKSGSNSQINEQSSESLQDFEQLVLNIDKKWFYEEYIQGQCFFFCDDQADQIMRLLKFKAKFLHQNEDDNISLQCELEIEKGFQSHIIILPIILSMKNWKLYAKMKGLQFFLNLLKEFVEQNFPIIPQTTKILIYKGQYFDMLLESIIQLISVIMKNICSERKLVGVKLYYTKDVQIKKNRKMIQQYYQKQRQEQRQYYFKSLQFFMNHYDIRINKRASSIDLNLKGLMGNDIAKQKLKAAENAVLFAIKAKTSIQNQPPKPPVIPLMLFDDDNVQSPQILEVSMRDPDVNRMQLKNIINPQKRRQSIQEQEMNRFSNLFQQNQKVHHQRNHSNLTPKKTSESIFKKTFTIETNLLQQKILTRPQTIIQQVDQSFSSKSQQSQTSSNNQSKDHDLQPPSISHSVSLSQQPPPSFKKKNVGNVSQSQQGNTTNSVQNLLDTQQNLVAHQLFDTQALEGLELIPEQENELEQKIITIYQKDDHFTVSPQWKIKRKATIHNFQFEYFWNQHKQQKKVFQKIYNNPFNIKEILMVGTQQIDKQYFLISVTQILKEQIQIFNQSVLNNKELVKIQITYRLAYPYDNIWQDSIVIKFKEFIKWFVPDFELSSYIQYFGLSKNQKIQALSYLGRYAHIKESKIKLEYYEEKRNKNKIKQQQRRDIEISRMKQIESMLIRDNMDLYYIQILGLNNLYQELLKERKILEEKIKRKEERLRLFLQLTDPNKLDPTTQQPKYIGYDYDGDSEEKTIQVPLNLQMQILKSYNTVVYLKQQKQIIKIQSTYLIWEGDNIKMWRPFQRKIWQQVKIENKDKLINAIQCKLRNYILKQIIVKILSLNGKKYIIKQQKQFVQNNSSQDLLYQYFQLKQFSNQQFYKFLSILRVQLLQLDDCSHLWNSYFKQYKQLQHCYISQIGDCLLEIIVLIDNFAQVLNQNLNRYVFIRINIYEKQNMKTKMHKLVLTLDDLHITSKKQYDLYNENITQIIFDDVQKLMHKYVSYENQGVTKNPTLVIKNSKLNRTFKLVNSLPWLHNFNYDEFQVTLYPQKFQFYHPQGLHPKIICKVVKFLKIPQVFSKRHHSFYDNYEKQYDKLQEDFTFRVPCIITIEKFQEISSFYIYIYFPQFCRRFITKLHFDDILINQDFNLKLNFQFKEQLRIHRSEKLWQSLIESFKIVINDQKKLILQIKKFKLSCILQEVVYQKIKQIEKSQESILFIVNIEKKIEQDPEQLLIHYPFERMKLQEAKNYSMFLKLQWLNQYDNIQSYRLPLYELMIGYFEKTIKGFNMFDYKFKLVDLIRMCQISVENIIMNFSMNQTINYNNTLDQLMPQQLTYNINQTIKLNINLKTIQVDKSIYKLLYRGVLLRKPSILVGIYQKLHKQQLYFYIQRAKDCESFIHKIRFQEIENIYPGFQLNLQINQRSIGPNIFKILKNRLIVQSYYQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L8V2</accession>
    <name>A0A8S1L8V2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">IBB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0320295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8062033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L8V2"/>
    <dbReference type="OMA" id="MIKLCWQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IBB" evidence="1">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="467" mass="55533" checksum="7B540FA9F0E2BDEC" modified="2022-10-12" version="1">MNIVQKKREEFSINIRKQKREELFKSKRISNSQYLPFSYNTTNLAEIYSNAYYQKQCPNNVQLNETIQKIFKISELADAFNENECEKNYDTSSDVEIIFDIINKGYPVLDRACLQILLSFTLPSHFTCKPILKYIPDLLKFFNETQYDELKQTVLFVFSNLAADCYQCRDAILIYEIPQAVFQTIYQTSRELMDDLAQILINISRTQPLMSNFNQIQMIKLCWQLFPYLQGNEYIAGILQLIQKICQKDKNLLDMNFLNSICQNYSNSDQYLNEILALIKIMSYFDRNRYETIENNILNYLILKVNQTLITGQQDIYLQKQINKVLSIFTNMIVENPKLIFNTLNTNIIQLFKFNEQLQETYNEIFSSIYYYSFYYQIINNLNQNQLKQFIQLFQPIQQMIILLDYNINNTDIIIDILRIIEMIMQKNIESISVFVEQNGEQYLAELSQHENEDIYNLCEEIFEIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LC82</accession>
    <name>A0A8S1LC82_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0370126</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GOT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LC82"/>
    <dbReference type="OMA" id="LMGWPIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF9">
        <property type="entry name" value="GOLGI TRANSPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8065157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="139" mass="15759" checksum="9BA0D43C83462D51" modified="2022-10-12" version="1">MYFLDDSKRIGMGFLTVSLLLFILGVMFFLDRALLVMGNLSFLIGLCLLIGVKSTLSFFLKKGKIKGSIFFFLGFFIIVIFRLSIVGFPLQIYGLFQMFKSFLPFLYDSATKLPIIGRYLRNPQLKKMVDEVSAKGPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFW9</accession>
    <name>A0A8S1LFW9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFW9"/>
    <dbReference type="OMA" id="IHANLNM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="426" mass="49479" checksum="6ED7C0BE744D69D3" modified="2022-10-12" version="1">MYQDIDFNDQNNNNVPKTSTEHSNTKSSDSQRKLSQMKYNNKENPVIIDNKYVLLNVIYQGKTSTIFEAINLQLQQACVVRMTLISKSDNSTKILQYFKTQFENQFEEKYQSGKIVQNPQDCLVRLIDHGVFMNQYNYQVLNKTGPSLKFWFNFWKSRFSFECIILIILKTIDALQLLHSGNFIHANLNMQSLLTSLENQKTLSLGNLQQAILFKPNSKIGQKCQHLNKYSSLGQHLGIQIYPKDDLESLLYIVIQLLTDGEFFDQQKNFKTRSDKLQWYFSVKHSFLPEKVLKNYPPCFTDFANKIKFLNPMELPINYDILKSVFKGCKNLEFDWNPLINGLKKNSGNTSQQVSMQNVDLKSGQLSLDTINESLNEQVIDIHHSLVKTQKHIGDYCDFNPETQESDGEISFEEESTDKSYVYCFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LGS8</accession>
    <name>A0A8S1LGS8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0370270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LGS8"/>
    <dbReference type="OMA" id="LHSTHYN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07830">
        <property type="entry name" value="STKc_MAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000545">
        <property type="entry name" value="CMGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000645">
        <property type="entry name" value="Probable serine/threonine-protein kinase DDB_G0268078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8065435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="351" mass="41042" checksum="EEAB727845A790D2" modified="2022-10-12" version="1">MDKYKIGKVLGDGTFGSVYKAVDETTGQIVAIKKMKHKYNKWDECINLPEIKSLLKLHHPNIVKLYEIIKQNDELHFVFEFMERNVYHLMKDRQKPFNEIHIRNIIYQTLQGLAYMHKIGYFHRDLKPENLLESNGTIKIADFGLARDIRSSPPFTDYVSTRWYRAPEVILRSNEYNSPIDIFAIGCIMAELYRLWPLFPGTCDTDQLQKICEIMGTPSEQDWPDGYKLAAKIQHRFPKNIQPKPLSQVITQASDDALDLISQMLRYNPLKRPNASQALAHRYFIVALPILPSLDNIEQQQDQDYTNFQQDDQQQDQRFGPQNQQNISLFYMKNARYKPGYTCILENAEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHU8</accession>
    <name>A0A8S1LHU8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0380108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHU8"/>
    <dbReference type="OMA" id="LMINQCP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013887">
        <property type="entry name" value="UPF0592"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35397:SF1">
        <property type="entry name" value="ARMADILLO-LIKE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35397">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08578">
        <property type="entry name" value="DUF1765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1002" mass="119202" checksum="CC6197BC545DE17D" modified="2022-10-12" version="1">MGGVCGNHHHQHQHNQFAKSPIGPHRTGCSFMNPERMKFNIIFENLRLQYPTKSCHLSIKLAGEILMETPNHIDLDGKHAWKKIVETQIQANILELHEKKLEFYLLEEKNVIATLEIPIFDIISGPIHFDYSIGKGRLSFDMQMAQILQIDINPIEIDCSYVESIKDKAYVFNLRLVTRKMYFTSPNSESFYNPAYLRGFNSSLNNFLQEMMFRTTWNSSSELPIFSIELPSNEMYNSSLQVCIWSINKGNEFQSPGICSIKSLKEKCEKEVFIDQNLFAETFIALHKLIQEDDEEDFQMYQQFKTTVTKSLWCKGNKVGTIQCKFNVKLPRFLKQKLVGIRTENGCTLGVNICSSKSVAQIAEITNIFQKLYESMFKMQSLSHNDPNRTILQHQALTISQQLLIEVQKADKDTNSKLFYYKHQEDLFKAQEYFIKIAEQITKYIDKLDENLREVCYEILLVIIDRGEFRLHSLGYFEECKQLSKKQLELKNTVNLHYSEFMLQTLNWVLLKVPIKTSQQNERKFMMRFLVLSFIRIYNFKDQLLKCINKPNDPQLVEWRGNEFQLEEQDVFINEQVAMIFDWQTYFYNYVTQNQQYQLSQSMYDEQWKLTLGKRSLIYQYFIMDYCTYIQQILQKNNIQWQHVPGYKQLLKSFLSELKLKESYTASFFDCVMAISRNTNIINIIVMILFNKTNLYQSDQVIQVFDLLSLIINQCPQTLTVFDYPFFLNGIRIVLAQSEHAIAIATVLELIYTNFLKFPIEFRKAIIDLLFEPICFELFLHWSKTVRTVFMSFLLYRICHQYRNNKISVMDEELFEQQYLIASKPRKNFSFYENRKEEKQLIADYIYLKYTRFMMNIEQVKIQLKQFPIHKDLQVLQNLKEKLAQKNQYHQENQNIIIQEQRQDDKEEIPSEKQIIFERRNEYRNPTKKRTIILSDNKYKYLKVALNEFSELTKQYTRWRYQSMNMSGEELSPEEKGQAYLNFQVPQIKILVHYDQKEGRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LI50</accession>
    <name>A0A8S1LI50_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LI50"/>
    <dbReference type="OMA" id="IARRWSY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="392" mass="46197" checksum="FB005C9FF272273B" modified="2022-10-12" version="1">MIHQNLAFGNLITSNQLERLVKQQKILLKSPQEKNDKKIKSKRLFRSTSFKTEQHALLLLTPKNRSTKSPMLLKPPIEKNALKRLPSKPNPYDALGKSEKSFTSTQRRSSAPLLKITRRWSYHETLTEKQRRLNNKAPKTTIKYHYITAEKAKKKKLNRLRWQYKFISRLQKNKIKKLRSKTLPLAPFIRMRHTRAKTIAEEQYHGPILNLEESLIRKIDILVMQRKSAMRRHTCFQIPKKSVLVSKEKLINYQKQISEYLVKTQQEPENQVEFSPALDQIRAYQKRVNFSDRKQSLKISHPKKKSFVSEMIKKQSHSIITSYISNKAVTLNSEHQSISYTPLNQLIQKKTHTIHEKIVNNNQTWSDKTLIKRRPNLLIKLQPYLSKQVVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJB2</accession>
    <name>A0A8S1LJB2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0410187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJB2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="247" mass="29963" checksum="624B99F7F1AD1A6A" modified="2022-10-12" version="1">MIQLQIHYKVDFGQALYISGKWNFEKAIRINWTWEDIWISDVAYHNMEYKHFISKYDKVQNIYLESGYYRVTNKHSFDVWNHRKICFQCSNSKNLQIYIEIIFNLEQYYKAALLIFQDGLTNPKKMVYLLTKNIFYGYVPNKYNDCLVLKKANLKTIIEFCNMKEQSTLEQKTINQEIVHLIVDLYYFKQESFTQSLLQLIQFLIQKYQVIYINIYYFQLLYISNKSLTHLRKYLSAYEKLTFSPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJX7</accession>
    <name>A0A8S1LJX7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0390260</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJX7"/>
    <dbReference type="OMA" id="WIEPRQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210:SF1">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-CH-type" evidence="5">
        <location>
            <begin position="173"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="4">
        <location>
            <begin position="297"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8066715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="44197" checksum="39D85823D2C8CBE7" modified="2022-10-12" version="1">MGNCCKQTGQKNFSSIQLNDLLQRKSLLLEQLEQLQNPVYNQTSLQVKMITWIKDSHDLFDYENSNRITQSLTINSSIKLYRDKTSGKIQSTQQGKTLIRIENRQNCFWIEPRQIKNDDHHVWLVIKSIQQQDNGVKLQYNDVIRVGKVEIKLQEFHFDVSEKKLDERETILSQSVDEQRCRICLLGSESIDDPKIEPCNCSGTMSLIHLKCLQHWIVTKYNMESSNAIVFLWDLMKCELCQSNFKRKLQLNGQTVDLVELSKRQFQSYAILEMKKPLIKSKERLTYILNLEKLEQFKIGRANDNHIRLCDISVSRFHCKLILHNQEFYIYDNNSKFGTLLKLKQSMPLLKEFQNVQVQVGRTLFEFENVSNSYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LL33</accession>
    <name>A0A8S1LL33_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0430080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8068908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LL33"/>
    <dbReference type="OMA" id="DQTEMFT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8068908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="321" mass="38568" checksum="DCF2306026FD779B" modified="2022-10-12" version="1">MEKQFETNIRLNFIQQNCLMNFVQQIKSNFGMIQRKEIIAKNKKKVESQNQPQLIFSIKRRQNDQAFDNIFFSNEEEMLNFLYSRKQKRLNLDELVGNLSLNEKNQEIVGLQRIPLQNIDSDQNFDQKETAEKSLIYFKRDRFVTKFRKDFRDSLLSNNRKKIIIQLEQNKNQLTSVNEKANYEFEEDYYVIQKLEKEKVQSQITMKIDTKQIEKQIAEQQFESDTEKSFDSEDSQRTDYDDYDSLDQDSSQQDGLEEEEYSNNNSDDSIDLKRFQQNYIQQDEFEVDEKDEGNQGDQGDQTEMFTSFIRQHEKIIKDKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LLL1</accession>
    <name>A0A8S1LLL1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0430021</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8068830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YTSHYNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035802044" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="1783"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1693"/>
            <end position="1717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8068830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1783" mass="210391" checksum="01301B3A1983CD29" modified="2022-10-12" version="1">MLILIFLNFIVGILCVNRNESKVILTDFYNNHTPVFVIAPYYESYTIDLFNSKDVLENTSFSNVNSTNQNLTFQITPFIETSSEPIESPFCPVDEWGPLYQPARFYLSQTAIELLFYNQSDDKIVQVQNKLHRDLFDLDENFQNISCYSIFQLRKNNQFGVDCDINKNATILIFDLVFDEQTLIIINFTYNTYYYYTYEDEENENFLKKCQYRKTINANILDVANEIKFEYLQYCPSLFNQKAITNEFLIIYHERFNRHNKILELQAFKANIQDLNSCKMEQNTMIEQIFINSLINGFDNSTWNIGFLFQSSPTICKCNVQEIYTSHYNIKCNSTLIELYQPNQIRLVQNLNDEVVLVTGTNIAILVIYLERSYQSENFFTISYKQIFEPGYLLVDMSITSQYLAVIMTDSPFVQQMSPFYSKPNLQCSKQASGETKNILKIFYLNAEYKTVYYQTNSFNQFQSFFQSLNQIDFGNFILLYNFFDKKLNIITLFEKQMNVSYLLGQDNLLQTYNQQIAPIIISFNISGGYFVYDQFQDTYNYNVVNFSRYIILCFIKLEDSSFQLIAQVHKLEQKEQNSLEQCPISKDILVFQEIINSIDLFEGIMGPFLNFNYVPNYNISRFYPYYQNHKAVIKQNQFYDKHNQPRSVFNVLSAVNKNIFVTSNIFGIVEILLTYQNNNQTIEKLETSQSIIFNKTCIINAREYQSFEKQFPINIEIQQCTEVRYRDLHKYLQQVDYYKQFEYYDIAVQYNNSIIVICQKIETLSGRERCQNITDMQKENELILQIVILQGPIKNERVSYFFTILYNNTQTKLYFYVLDVFLPSQFNPDITYHNCSCYNTLETTNTIISFRYTYINQQTLFILFSSSQIVVYTPGPSKLTKEDKLYNFTSTLTNVVLNITQCQNNNLLGFEITKYFQSKLAKQYLIVWCQENQNGNFTPYLLQYIITNINQINFWRRLPHYDTILSSKTKILISGLILYVPVITKSVYNDYFQNYYIYNLEDYTARSLVEKIEIEKKKNELIITYPISNLMDSLIAGSSISLTLIITEKKFEIYYLNQKAEFIFHLQQQFIPVYNNSPFVLVIGVFQPQKTKTMFRNYTLIYNFTSTQISFAYDSSETYKVEINKGQYIEAGGFYQYLFLVNQSIFNGPIASYNHSSNQVNLSIIPSPYLQMNKYLGSYMNEVSQFGEITQIIDVGDEIYTSLFNQQIQNISNYNELIIVLTDYYVLFYQKHSIPDENRSCIYFDYTQNQQTTNSSLCSIRLIYYHQFKPFNSFLFCQMQLNGVNVLIIVCQLKYFHKLNDNLSFQDKENIQQYIYLEYTYTKLTNNISITTYVMNNRQFCFCYFNDLQPFLYNNQVYQMIKIGYQQLNSYFGIYQIIITENQTIQFIQYNSYPNQNNSQPFQYSEDFALSIIEYQQIQFLYLIVLQPNNIFVQVINLTTGFPAYGPGQNLIFQEDEEYVRFEKAVFIQAINHFQPHIVNESQMDQTLSVGNNNQIYEITFNVLTFQAKLQYTFYQYLLCHHNFLSRPQIELSYQNDTVFYIVSQCLSNQFEYQSVQVEMTYLLNELNTNIVYTSLFIRNYSRNYSRPVEILEPYLVNPQELHFQKIQLYQSDAQANTSKSHLLITNSTYFLIDLILYAENGYYLYYPMLSANDDRDHSVYLNITAYNSYSSCEAQFEIGWIQQDSQINNVAWFYATLSIILLAILIVIIVVIYGFCQKQSQIGKKDQNLPLERIGDDESEYGVELQGAGYNQLMLGDVFKDLLRDNKSKKQTISYTDELLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQW3</accession>
    <name>A0A8S1LQW3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0420105</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQW3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="6">
        <location>
            <begin position="29"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8068331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="27124" checksum="091FBA87B54F120F" modified="2022-10-12" version="1">MHQYRFIYHLLIYQYQLLYKKWNLVEPDAEKIMMAFDIMIFAYKIIGVLTEIIVEMNDTISFQKFAMILETILYLFFGITSSLLFYSNTDQSIITNFQKEYKGNYPIEVTLSILKISYVYQHFNSQYASLSFGKNKSRQNQEIHFKNHLCTLLNGIINLIIIQLTAFCILNLLQFYLQLDVFVLYHLDTIIPYGMTFHYELKPIYQIINIIVVLFRILGGIFGIITCFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQY8</accession>
    <name>A0A8S1LQY8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0420120</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Neuron projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQY8"/>
    <dbReference type="OMA" id="CSEREYN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14509">
        <property type="entry name" value="PTP_PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000029">
        <property type="entry name" value="Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045101">
        <property type="entry name" value="PTP_PTEN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF81">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE AND DUAL-SPECIFICITY PROTEIN PHOSPHATASE PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01301">
        <property type="entry name" value="PTPlike_phytase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="11">
        <location>
            <begin position="109"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="10">
        <location>
            <begin position="216"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="12">
        <location>
            <begin position="287"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8068361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="441" mass="51751" checksum="36E64B0671933854" modified="2022-10-12" version="1">MHSATQEELKSRAKQNEISSGEEELLSDSGDECDPENYNFYMSQIDFAALANGIGQDDQKGQEINLRERVSTQQLRQEVNQVNQHISGNHQNITSVPNPIKLLVSKQKRRYNYNGFNLDLTYITEKIIAMGFPAENIESIYRNSMQDVRRFFDSVHPGHYKVYNLCEERKYDHSNFNQVAEFPFQDHQAPTFSLIYEFCLDLDNWLKTHEKNVAGIHCKAGKGRTGVMICCYMLYAKQFTNAYDSMRYYGMIRTKNKKGVTIPSQIRYIFYFEKALNNKWVPDNMPNKQVELVKIRLIPVPNVKFFGGCAPWFRIQNKDKEYNSKNQFPVKEYKLEPYIEFKLKDIILQGDVLLQFLNQGIWSRSEKLFQAWFNCDFFDYTGVLMIDKFMLDKACKDKSGKTFQKDFRIELHVVEVNQDNKSLQSLYNNTINQITHKNFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LS68</accession>
    <name>A0A8S1LS68_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0470044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LS68"/>
    <dbReference type="OMA" id="LMMLNTK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="176" mass="21385" checksum="2FFE41C7F0A7E58D" modified="2022-10-12" version="1">MNLQFQLELKLKDFEYRKLQQQLNVMKSVVDEKAMKFDSIKKQLTETFSALIKYVQLELQNLKKELLCLISTQKQEIIQFQNEQISLKYIQELEQLNQQYYEEKEQFKIRIDKLTKQLIESEKNEVYLMMLNTKYIKENELLNHQLLSKSRQMSQTEQNFYPNKTGFISKFLNKYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSS9</accession>
    <name>A0A8S1LSS9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMMECR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0410175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSS9"/>
    <dbReference type="OMA" id="LFITWNK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00296">
        <property type="entry name" value="TIGR00296 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF0">
        <property type="entry name" value="AMME SYNDROME CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="190" mass="21654" checksum="DB910049B91994D3" modified="2022-10-12" version="1">MQINQALPIHCAYCFDVLIASLQQKDVPKPTFQEFDVPVFVTFHANKEDLRGCIGTFSPGPLGQQLAKYTFLSAFKDSRFPPIQTKELNSLDVGVSLLINFQKGKKWNQWEVGKHGIIIDFQEGGREYGATFLPEVAAEQEWDINTTLEHLIAKAGYRKNYQNVLDKIDLTTYETSKAKLTYAEYLELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTP7</accession>
    <name>A0A8S1LTP7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0480183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTP7"/>
    <dbReference type="OMA" id="QIPYPQH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="748" mass="88222" checksum="CF8F43F318E64EBE" modified="2022-10-12" version="1">MFRFRRAINLFNRKDGFITKLKNKLLKDDEDEKQIIPHQTIKQPLNVKQEIEQFITQQTEISDLERIRMKNLDIKLGTNTYFHEIQSYEERLDSDNVMRRQVLARRELQKEKDLAVAHLFNPDPQQKVKTPWIRNINDFTSEAIKQIPYPQHPKLFLEGDFFRYFEDKHYEVDKVAQFDFSESLYTQLVQIQTLRREETVKHRYLELLDYYQNIPIVFNDYTYFSKDSKEGHPIYNIYKRKNENKELIKDDDELVFSGKIIADLIPKHYEDLILDIEEEKFQSLYKEFLPNKSHDTYGCVIEQLGLNFLIVRSDKIQLVLENVDNTFTFIENYLYYIEKSDLNLQKYVLKLNLLNFEERTIVYQISPGENVKIRNFGIGQAAIEIDMANENKFIDLNGKDIVNYKKGYKFNIDQNIVNTFVSSASLEEQPKEIRTADGHIVYECKKGNIRSFCANDHFLAILERDLQSQISDRLIIMKEKKNHEMVIQESQYSINLESNQIKESNCLYFHLICPYKPFQILKYNIDIKAISLHFSEQLPGFDYNKLQVDTFKITEEFNVCVSFLQDLPVQFVLIQLIDQEQYQSLNQEDVVLYENGCAICQIIVNPSCQQQVLDNLQKVFQEVSVLSDKVILKTKGALSGLIGSYTHFFVQQFYGFLCHNGLFDNSKNMIDLPISSYDITKVPSLRNTFISYTLNDELTYQSQKMVALLRENKDNEVDLKKQFGVVLQQCESDIDEAIFRLTFLMSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LU80</accession>
    <name>A0A8S1LU80_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0470247</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LU80"/>
    <dbReference type="OMA" id="DVPDQEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="235"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="480"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8071570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="527" mass="62067" checksum="900F39525A6F50E1" modified="2022-10-12" version="1">MSVHTSPKNSFLSSARTRPQTAVTSPRRPQLQLSIPNDDQDQQQLINKYTATTSLTPKSLMKLQERKVNVGVRSENVSCNLRVTKEQAEIDAPDDYFEKEVQINQDEYEQFKKNFHRIVFDAFGNYIYTKPGPIDQEFKLQVSSALNTHKHTSSSLKKKLQIKTQSFQPKKTIDNLLKSTSEMAKMNPRRRAILEIKQKAENSLYQMINNLAKEKVLSESKAQLEQVTQQMLIYIDKFTRENQELVDQLEMAKGKIFELQSSNESLNFKNIQLLKEIKHSKQQLDELKRSTVEIEKFIPQFNIMTKRFENFQAEKFIDKYDYFENQNLVLTKRVSDLELDNIQLEKQILHLQKDLEIKQQINKHQYDMNFHSSTNKQDVPDQEYEQYKDMYLTLFKKILEIYSDWTNKTKALLPDKMDGGPRANLVEPLEMLQNMEKMITISSNEKLQSYLRKIIVSANQLQRKYLTENVNEKFDPDKIYERIIKLVDNLKAQISNLQCQVQSYKQTQNCQTQVQTQRSKSFLHEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXT2</accession>
    <name>A0A8S1LXT2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0510160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXT2"/>
    <dbReference type="OMA" id="PVYPNAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="273" mass="31124" checksum="BC931D6B651BE2EA" modified="2022-10-12" version="1">MSNRPQAAQQPQQAPAPQVQQRPAAPVYPNTYLPPTYYPASPIRQSYVAPVAPVAPIQYAPVAQMPVAPIASVPVQQLAPVQVQPVAVAPVAQHQTIKGESRIEYIPYQKAVVEYEEQEIVSYVPRETKVTDYYAVEYQTEYIPQVFQEKYTEYVPVDRYQERVEYYPVERQVVHQQPTQYVQQAVSVVQQPVIQQSVQYVQQPVQYVQQPVQYVQQPLIASRVVPQFVQQQYAAAPVTRPSQPLQQQQHQPQQVPSQQPRSQAAQQQPQQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LY49</accession>
    <name>A0A8S1LY49_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">DNA topoisomerase</fullName>
            <ecNumber evidence="3 7">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0460193</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="3 7"/>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="VIHNVYS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.290.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.140:FF:000003">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF21">
        <property type="entry name" value="DNA TOPOISOMERASE 3-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="9">
        <location>
            <begin position="14"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="10">
        <location>
            <begin position="176"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="615"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="625"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8070895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="646" mass="75144" checksum="E3B8BD49D5CBB1B8" modified="2022-10-12" version="1" fragment="single">MNNFKTSTKIDKIKVLNVAEKPSVARSIANVLSKDHRIEETKSPFNKLFKLDYRLKDVEVEMWITSVSGHLKNLKYPQKYQNWEKWDPIIILKEAEIENNISNDKQNLELNLKNFASICNRLILWLDCDREGENIAFEVMEVCKESNPNIQVYRAHFSAVTYVDVRRALDTLKYPDENLSNSVIARQEIDLRIGASFTRFQTLLLQKQFNLTSIVSYGPCQIPTLGFVVQRQKEIDSFIKEQFWFIQCEDDQKCIYNWDRTHLFDEFIVVLLFERCYQDYATVINVQQKDVQKWKPYPLCTIEFEKLASKKLKISAHKAMELAERLYNKGYISYPRTETNKFPPTINLQTIIRDQQNHPIWGEYATNLINFAFEYPKAGNKDDKAHPPIHPVKMMIESEAQNKTEWDVYQLITRHFLACCSKNAKGSETTITLQINDETFSKTGLVIKEKNYLEIYIYDEWSQSQVPNYKCGDSIKIKLTLQKGSTSPPKPMTEAELIGLMDKNGIGTDATIHEHINTIQDREYAIKRGQIIKPTRIGLALVESYEILGFTLHQPFLRAIMEQRMNEVALGKKDKSQIVQATIDEMTIILKQLQEKQKLIIKTFGQYLEALKNYDEDNNNDDNDGNNNNNNNNNNNNNNNNNNNXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZQ9</accession>
    <name>A0A8S1LZQ9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor 1-gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0510126</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZQ9"/>
    <dbReference type="OMA" id="VSPDWQV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03181">
        <property type="entry name" value="GST_C_EF1Bgamma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000006">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1010:FF:000001">
        <property type="entry name" value="Elongation factor 1-gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001662">
        <property type="entry name" value="EF1B_G_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00647">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01183">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50040">
        <property type="entry name" value="EF1G_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="85"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="EF-1-gamma C-terminal" evidence="5">
        <location>
            <begin position="276"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="215"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="215"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8073560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="51613" checksum="8DD26C2AF402D84C" modified="2022-10-12" version="1">MSFKLYGPSTNPRLSQIHLVADLLEIPIELVEVAYNQCDSKEHLARNPFAKVPVIETTDGFIYESNAICRYLARSKQESGLYGATPFQQSQVDQWIDWTVNELDPNFMTTFTQIWGHIPVNEDSFKTSRNIINEKLKQLEAHFKNSPYLVGDKLTIADITLIVRIAPFFTLLIDEKTRKSYPSLMKWFTAVTELPQFKKNFGRVRLCKVAFPLPKQEEQKPKEQKPKEEKPKEEKPKEQKPKEQKQKEQKPKEAEKPKEAQKQKDQEDDEPAPEKKKNPLDLLPPSPFNIDDYKRAFFAEKDISKNIQQLFTSVDTQGWSLWIITYNKSQNEGKQIILTNNLMKGFINQRLDQNFRKYSFAIHGVYGDEPNLEIRGAWIWRGIEVPQEWKDHVAYDYHVFRRIDINNEQDKKDFTEYWVNQEEDVSKVGGLTARSLIYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M456</accession>
    <name>A0A8S1M456_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0470096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M456"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="270" mass="32722" checksum="BAD2FE758E0B2175" modified="2022-10-12" version="1">MSIYEQELQNNVEKLVDNYMKKSKSFHRQRSSFSMNEEQRDFTQEIIEKEFQKAALLDDLLKQIQLIVDNLIQRHLFDFQNQLLHIQNELLLLKSKQSTVGSQESTMKQTLVLSKNSSFTDFNDQFIFEDQMKNLKNKMKNLKEEVNKKTQHHDSLINMLLDQQKDFKENQRKNQFNVDVKLIEKRMHDQLWLSVETQSRDFVETKFYQLKQMVEQNNQKHKEKDAIIWTIINECKSEMDKMNQKLNGLNCLLQEMKENNHWYTHIWKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5J3</accession>
    <name>A0A8S1M5J3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0500182</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5J3"/>
    <dbReference type="OMA" id="PHFRCSS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051568">
        <property type="entry name" value="LZTR1/Attractin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376:SF1">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="378"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8073051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="531" mass="61483" checksum="8987A726B973A558" modified="2022-10-12" version="1">MDYCIRKTEMTDNKEDDVTFASYEWENIKATTASQLPTCRNCHTATTFKHYMIIFGGKEGEGRKKFCNDIHILDLKRLNWTSQIKVSGQIPDVRMGHSAQNYYDKIIYYGGWNGYTVLDDIIMMTPSEQMNVVCIDWQYVKSENTPPKRQFHTANICGDVMYIFGGGDGKMWLSDLYKFDLVKCYWAQVDTTGQKPQGRLQHSSVIYDHKIYVFGGEPDRSHQLNDLYQLDIGNNVWTRLQPKGSTPSPRVSASAVMMNNKIYLFGGYDGQQWRNDVFMYNIIENQWEYIVINDQEVLPQFRGQTKENNISSQQSSPPRPRCRHSAIAYKNTIVIFGGNDSEKSYNDVYMLKQQSTIKLAESTLKQDFSSILFSPILSDITFFVDNQEIHAHKIILASRCEYFKTLFLNEQFNLGDKLVITETTINVFKAILQYIYTDEVLIDSYIVYDLLALADKYMLQRLKNLCEDHLIKNISLKNVIEVVNLADKFSAQELKANAMIFLLDNKQKILNTQDINMLSKEILIELLKFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9P5</accession>
    <name>A0A8S1M9P5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0530102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9P5"/>
    <dbReference type="OMA" id="RIEFNDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20855" checksum="300CBC126E03395A" modified="2022-10-12" version="1">MDPIFYTFLGIGIGFFGSKIVMNNVLNNKQNKNPQQNSHTKKLIPSTPDFKKVFQQNQIPQQIKGQIELDDQIRKIIKIQSTPVELDTDSQFSQFKDQENNKLNQQNISDHHMQPQTPNFQNTAQAKPNIKLLVTDDQIQQVITSPLILQQNKFSTSQNNYPQTTQSSSHTESSGQNRDKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MB17</accession>
    <name>A0A8S1MB17_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0580159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MB17"/>
    <dbReference type="OMA" id="YKLPQKP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032054">
        <property type="entry name" value="Cdt1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014939">
        <property type="entry name" value="CDT1_Gemini-bd-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08839">
        <property type="entry name" value="CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16679">
        <property type="entry name" value="CDT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDT1 Geminin-binding" evidence="1">
        <location>
            <begin position="74"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication factor Cdt1 C-terminal" evidence="2">
        <location>
            <begin position="245"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8077530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="360" mass="42459" checksum="33463DD3D831A748" modified="2022-10-12" version="1">MKNKNINAAPELNQNLQKKVKLCEDSQCTTLFNENSNSVEQFPTLSKIASKYLSKPSLRERFQLINETRNLQASKYYSLIQILSAIDSQLLFLKQRRESLFWPNIVKSCYDSTQLQVTTRQLLEILTVWQQSYIINWEKFEKQNGNYELLFKFPDKQLPNSQELNQRKELFKEKLESYIQLNGDFIEPYKLPQKPSLSNINLIQEKNISGKNDCVQFCQFINISRIFQDKDKSNESKPVSNVSQKLIDKLKERKLLEKQKYEESQDDKQQSSKKQHVLIATMLHQYYQQRDVSNMFFSNVLKYIHEHNLNVLMSNDQIKIIIMNLIQSIENWLQLIDNPGGQILRLNKGIDLPNIINQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MMK1</accession>
    <name>A0A8S1MMK1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0660130</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MMK1"/>
    <dbReference type="OMA" id="VAKINFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641:SF19">
        <property type="entry name" value="NEPHROCYSTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_6G03870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="304"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8081708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="47214" checksum="FBEE9BC874114E3D" modified="2022-10-12" version="1">MNVLEQSLKQIQELYFKGQYRKAYEQLQKLGSDLETYDQKFQLFQRQECKIELLAAFYTEYGSVALKLSLLDISKKCVQCALYHLKDFTNMNLFIMNILTYQRINFWLSRYHDSIYLLKRLLNIVPNSLIHYKIEIFINIANSQRAWCIYDEAENYINQAEQLANLLSQSHQNDLLKTNILFLKARLYMDTDRSKYGLDFALDCLQQREMLLPSDHPDIAIAMELVAKINFIQNIVQEAQKYHDQAYMIKIKVFGLHNFETIDSINEKGRLLLSCKKEQQAIYYFQLIGEISQVLLGPYNQYQALSYNNLGCALFNIGKYESSIQYHSKALLIYEKFCGQQHAKLVFSLNQMANANKLLNRPKAAFIIWKRCQTILEKNPQINSKQLSSIKSSLQGLQIQEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MN47</accession>
    <name>A0A8S1MN47_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0640152</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MN47"/>
    <dbReference type="OMA" id="TFLKACH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8080762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="320" mass="37672" checksum="40392EC6A38F476B" modified="2022-10-12" version="1">MQSIKQLLIDILDNIDQVLSFSFQDVVESYQQINKLTTKEICGQSEIQQYEYILQIVAYLNRIKILFEEQLDEHINNDYETIIQNLEASIRSHIRVEQQQKLQIEIITQKLEEIIREKDLIIQQQQDKILSLEQIIKDQNKKLQETSPVEIIHKKTPSAFDRLGKFIQNKAQKNIQNSTQDTNFKTFLKAFHTDADRSISKGRRQLSAKRELGLQRINDETQLNPELKITETDRQRENVRHKLQRSDKTNELQHQSPEKQLYLNKYKGLSKFDIIKQEQTISSQSFDQVGKNLIALQKQISNGRLKNTQGTQLLKFLQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPG5</accession>
    <name>A0A8S1MPG5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0650112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPG5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8081222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="539" mass="65081" checksum="43E99E61C45C1F01" modified="2022-10-12" version="1">MLCKFHFLLGNYHRMKCIQCQIYNHYNELNKLHRSYYLNNGLFCIMNNYWRKFHITSKMDRSFHILNHQDNIHFCKINNLCFLHLNNLHMDVHTAGNQYSTNNIQGCMMNSMVLDKEYMRSHSNNIKVHKIDNLWGHQHIMYKMYHRVCIFYHHYRSIIFLDKYNLNKLLFYKNLQITCTFIFFINHISIITTQTGSSCISASDAIVRYYMKYILQPNPHKFYSQSSIPHSHHQSIQKHKHGMLKYCLSNHMLIGILEGNLRIKTNKLLKHFVMSRQYFSLQLEHQVTSGPLQIAHSEEHNQHPDPSQKYPSTQQFLQGYQQALQIYESKNIKYFQVPNILCKEIRNEYNFYLNHCNNIQQHNLCKLSLIHNYHMVICNESRNYQSSSILVNKINNELLHFHNLHKDCRMQRKQCPIRRIQQNIICKYHPNNNFIGKMNIQQHSNILYIHLDKLNKSFRHLSNNHQRKIGIEFQKCMFYIQYHIFYKLGYQTNIRCYKASMHYPLGIFLEDRMYNLNHYSNMTYNCLSNYNNVQMNSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWS4</accession>
    <name>A0A8S1MWS4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0660043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWS4"/>
    <dbReference type="OMA" id="PENTGCM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035734035" evidence="1">
        <location>
            <begin position="18"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="381" mass="43379" checksum="DF6577FE0AA551FD" modified="2022-10-12" version="1">MIIIVLISAIFCAEVKLKVESSPKDIRRINGRAEFDAQEYETSCQCESTTTQLHVGETFCDPTKVQLHQDFAGDDGKTASFEQIRHLIGPLNELQFGDPNSYHFSCLDGRNEQGVLGTPGGDSGEFLLALQVYEQTINTPLTQDTVDSILSNYLQYMKQDGFYMCTDDDAIRHLEKELGMTLTVDALIDPPNSFKDDLLTSLLKPENTGCMHLKSILKNPESYDMRPDLVRYFLRSYFTILWNKSNPLHHKLILEVMAGRHEERAFLEVRINEACIRQNMAPLLRPKRRITLASVPQGPTTTTFHEGGATEIFQAYVNHIDAASIRRKELSKFFAENSNHNGVHFEIDVMHHRLNKKGLMYLEITGQKIARYLPFYTVTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MYC6</accession>
    <name>A0A8S1MYC6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0740031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MYC6"/>
    <dbReference type="OMA" id="IILYCQD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8085230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="513" mass="59630" checksum="C6C6A4B58CF8A6BF" modified="2022-10-12" version="1">MDHDYFNHIRCPQHNLSINRIIQQKSINTSKRALCYKCPGEGASIFSVYQILDSLSSCLIPIHQQYKNLQDNLSTLKLEITNIINQVYSFIDGAIKKSEFEITRIEKYVYYRKNYKEFNIEDLESISHFIYNDAAQDNPINKSIKLLQEIQQCFKNQLIALFDRIIQLIKGSNQRQNINPILKPPKEPLEQKSTIIKKSNLVFVKKNEYQTSVVTAAIFNKNGDMLFQGQYPFSAYKNLEIKQIDNNFQIKNQSITEESDDNLDVQVTALALNDLTTQLFIGYSNGYITTYQFTGKVWNRKANFKAHTDQVNILIINLSNTELFSTGYDDIIKGYCCSQEIRYSNKEINSIKNICDIQINKESNYLFVIADGKLHILINSQDQFSQFQKISLQNQNVTCITTANNQLYIGSNIGSIYIFEQKNGSQFYEVHNNILIQQSFLQMKYCEEAKLLIVRLDKQVCIYQIGSNNKFDRTQEISGYYSLICIHNNMKFSQIILYCQDNYIASHYIMQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0A0</accession>
    <name>A0A8S1N0A0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0760029</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0A0"/>
    <dbReference type="OMA" id="FRHYIAK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005221">
        <property type="term" value="F:intracellularly cyclic nucleotide-activated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF11">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="608"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="648"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="686"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="712"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="744"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="829"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="345"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="967"/>
            <end position="1040"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8086148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1165" mass="139287" checksum="0C16790CB9B47588" modified="2022-10-12" version="1">MKQYINQLADKLQISTSDSVLIIWKVFINFCSFVIYIEISLELFNVEEFQKDNRGDIFFIWRIFILSLLIIDMIISLFIEYISNNGIIIQKPYKKVKYYFRHYFVFDLIVIVYLFVSTFQPLPFWVGFLVFFRIPSNLYSDKITEEYLIQFQFLLLCYRILKLLVTLLYIFDIFACSFYAMGLYSISIQEPTWLEYSNSVIGNIDEMHHVTQYIISYFWAVETLVSMGYGEITPMNQYEIIIGDLALVGCMFVCLYVISTILSFQSDNSSYLDENMLSIKQFMIAKRVSKKLYNEVSIFLSQFRIEQSKRDIAIEQVVIQRLPNQLKSKLYYEASLKIMKRILWIYNNFSNEFIEELSSCVREIYLNANTTISVIDDDDDPKIFVIDKGKVTVIFNEKEINLLYRGSTFGNYSFFTQEKEQFITYKTKSIVILQYIKLTDFLKTISNHKEDHEKFYYILDQVLFEKKYSLIQYGCYICTSTNHLSENCQIISIKDQVYEEFYKNPLRNSIQLRKGFIRQNKKHSKWILNRNAIQITHEYPISFDIDKRYYSKLLFQWHPFSKHQQERMNILLKDLLNDSLKQNDIIIMSENKSKNKKKSSVFSSSNQYLFYWQLFMMFVNAIFFIAIPQIIFFWNYYQQFSDQGGFKFIRIIFIILFVSDFIVQLNTNFYHEGILVKNRFKIAENYLRNDLVFDLIPILVLFYISLSDKYVIVYLLCLIKLIPFYRFQVYLSDKLKIMPQLRQFYMICGMLIEILYINHFYGCMFYGGSVYMYTYHPNSKTWVNDPTMNFGVIITKSTTSKYLYSTYWAIDAITRIGYGDILPLNDLEFMITDLTVITAVAIVAVNISLIENMRKNSKLQNYFVDNLWIQQYLKKKNCSDELKTQISKYLRYYHRIGQDRNIDIEQESLQLLPSSLKQQLMYEAYGIIFYKQHWLRDDDDVLFELSNKVKEIYLGDMENIFLDNIPDQGQALYYVERGYVELFINTQYSSRKDVIIAQLKKDTFFGHYSFITGLPRKSSARTMQFASLIYILRKDFHDVLEINKKFHHQFQTLRDSIIYENQFQLIGLQCFTCNGNDHLSIDCPLISIKKQVLLRFYHNIREKSQSGLNLRKLYKQNLKQDRRFKKRKKMKKSKTLRIHDFEFFLDYTIPRTTINYVIDKDLHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NB03</accession>
    <name>A0A8S1NB03_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0850136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NB03"/>
    <dbReference type="OMA" id="NNTMNTE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="177"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="395" mass="46301" checksum="3DC085309834E322" modified="2022-10-12" version="1">MSSYPKKRINFSELDDHFDDDQQIDSLRYNAEIDSAIQIKKSSDHEKLNEVQQLLQFSPHPQQENHKLSSVRCSFGLNSNNNFNPTRLPFSQNQNSVNSSIQIGSPDTASFPLQKKFLKLYQMRSSIEEISAENDLSQSRENMTKKSNNTMNTEEDKNAQFNHDETVQNELYQNDSYALFTQLLESLEQSQNQLQKQQIQYQELQSKYEKQQSQIIYLTKLNQEITKTLDETQNQLQLLNETNGLLKKKIQSYKQYSITEQSQYNGNTNRYNKENHDYLKHHSSTESTQSIELVNNLKKTQKVTNTYSNIIMMLLLMIQKCFYLDESIDKEGKEVITKITQLINQKQLDNIVFSIESLIKYFEQRFSDLATKNSKIQSMNNIMDQIRQLVDQRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NC43</accession>
    <name>A0A8S1NC43_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0780031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NC43"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="38" mass="4636" checksum="72136FA0B31D214D" modified="2022-10-12" version="1">MEFVSVRNCQINVIGISYLFMIKQMIDFIIMFRNLMEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDA5</accession>
    <name>A0A8S1NDA5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0860110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDA5"/>
    <dbReference type="OMA" id="NQACTYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="597"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="635"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="218"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="638"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8090588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="713" mass="83493" checksum="B51BBAC3631E7F78" modified="2022-10-12" version="1">MNNQQTISNNLMEEQVVEDQSYEQEEDEDLEQYTQQQNLKQLLKNSDSFMQQINQLNEQSIVKIKQQKYKEALKLLQQSEQMLEFAASCGKVIDRNLIIIILYNQACTYQCQWVLDKCSKYLDSVIYNMELAIKEDEQDLENFVSVQEKQAQLVKRQTFLAKAYLQYTAILSQMGKHNKALINSQKAASIMRELFKIANQFCQQWLQSNGSSGTATTSQSNISIRSNQQEQQQLDQHKQKRGQKYRMKDEIEFSRLVIDGAQDILKDMIKQVDLEKIPVNEKQLLKEAKKQLYYWRNNPENNEKHLRKELKLVSQNEEYRSLLGIQNIAEWIKNFNIGSIMHMAPQIYDDFTQFGEMIYELAKRQILEKVIYFSISYFTIATELRFIELEKAKQMGLKEDKIDTEEFKLSELYHLKSIEIACRHITTQSPYISHLITSYHKHYNINLDIIKEESILSNISEKQSIVNQESIQTQKQNQILQIQINNDNKNIIKQDQSPKLTNNFITKFLNSRSPPKTQQQSKIKNIIKNQADLIEQMINQKRGSDYSPSNQQNNHINQGQLDFSMYLKKYSNPCNTSNSNINSTQDFINSTMTIQLQPHKNNPNFRSNPIQQQSPTNNSGQLKSLLSDVCRTERQNGIDKQNSHTPISYRVNNRSPDSSYINSQKLKQQQLHLNKTPQNRPRTNTEQQQPCFPFKFETIQLLLKNKGNISKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEA9</accession>
    <name>A0A8S1NEA9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0780203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEA9"/>
    <dbReference type="OMA" id="EFLMNGA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="201" mass="22987" checksum="FF85C4F0FCB4B503" modified="2022-10-12" version="1">MPNDTFLKVLKKGPFQLSFAKTLASYLTRVLVTLHAASIAHCDIKPENILIAADYNLKLCDFGFARICKEPLRPPGGTPGYTAPEMYINPQVNLFKCDVFALGVVLFIVVMGFPPFQTNDPNVKDGWWGLIQSKQFDLFWSKCEQFKQQQLPQEFKNLIMSMLECDPEKRISLQQVLEHEFLMNGAPEEEVLQEIETRVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NED8</accession>
    <name>A0A8S1NED8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0730137</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8085084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NED8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="422"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="877"/>
            <end position="943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8085084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1072" mass="125179" checksum="716650F339A37667" modified="2022-10-12" version="1">MQQLFWKKIEIDNKKHGRPPKLEECVNLVYLIDQNRYLYFGGKNPFNIKDLQQEEKQPSPPKSDGISFKIGAPKQARVSSPFSNQECQDYVYILDPATYQWSIKKCTGKPPTRRTSAQVWYEAPLLYVYGGITYENKTVSDLYILNTEKFIWKRFFYLEGPPGRLHFGFSTQGIKKYLMGGASMPENLLMDDVWQLSFENVAWETQSLELPGITWEKLPFDDMPAIKAHTMIQVSDKELLVYGGFNKQKQCQDRCLLLDIETYDISQLELKGQSPGQRAFHELLVVKENLLCLFGGYNVNENQLKNSEPLNDLYLLNLNEGYWSKPIAGGYIPTPRFGYVMSCNQNEVHGEIMLLGGRMNDGTVDQCIYVLYELDMEAKDAWDEKDEKEKNKYDTYRELKKENKNITVPTLTLVFDEAEKIILEQKRTIGEKEKDLKQLQQNSLSLEQRAEKLQEQIDLFWKKIEIDNKKHGRPPKLEECVNLVYLIDQNRYLYFGGKNPFNIKDLQQEEKQPSPPKSDGISFKIGAPKQARVSSPFSNQECQDYVYILDPATYQWSIKKCTGKPPTRRTSAQVWYEAPLLYVYGGITYENKTVSDLYILNTEKFIWKRFFYLEGPPGRLHFGFSTQGIKKYLMGGASMPENLLMDDVWQLSFENVAWETQSLELPGITWEKLPFDDMPAIKAHTMIQVSDKELLVYGGFNKQKQCQDRCLLLDIETYDISQLELKGQSPGQRAFHELLVVKENLLCLFGGYNVNENQLKNSEPLNDLYLLNLNEGYWSKPIAGGYIPTPRFGYVMSCNQNEVHGEIMLLGGRMNDGTVDQCIYVLYELDMEAKDAWDEKDEKEKNKYDTYRELKKENKNITVPTLTLVFDEAEKIILEQKRTIGEKEKDLKQLQQNSLSLEQRAEKLQEQIDVGKLKMDQDLENTRIEITELNQKADQSQSTIDKILQLLAFERKKRKLMYRKALALEDLYRKTSLYIFEMDKVFVKGQSENLLETSINEDILNLIDQRKQEYKNILISFHKSFEERYKQEMQVRQQIRSNKDILKQFHNINEVYKKTLLKEDREYLDKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEX9</accession>
    <name>A0A8S1NEX9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0740043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEX9"/>
    <dbReference type="OMA" id="ICDYKIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8085254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="846" mass="100300" checksum="E95B442654644CAE" modified="2022-10-12" version="1">MYKGYIEKMFKEFQVQPIYLPFEFQQNGGEGNIIMDMDLVKQLDYFIKQNMNQNNEHTIQQFIRMFSVQLLLPNHVIDELLSNTQQTFEQQFDEEVIDSCQKQSINRIREQSQLEEMKFISQLQLQSEQKNEQEIFIDEMKEAKLIVPIANLKQYPLGRTQITVRAKLFNKPIIEKEQLQIIGDYCLQFRDESGMCQVFFTKNRIKDKFQFYYEHLQVQCIYDISEMEIHQFKQTYEIKVTSTTQFKLYTKIPVLQQLQSALNYNLKNGEEIIVAAMVAEIGELRQQNKKLILYTLPIDNNTDYYKIPLILWDQYKLLNFKVGDVYVFEGVLLKIYTDVIELKSLRTRFKVYTEEDEKYKDIIKQIIDKKHNWLQNDNQEIQIEQVVQQPVFISDIHQSINCSYFNTVVKILKDKPAIFQMKIQKCVKQLIVTSDNNNSQIIELIGDRLCQKVYICEEAIIFISYLKFINNKIITNENNTQIRVLPDDDIDFVEQISLLKQPFSKPISLSQNELEKDVLIKDQKRNSIKIKINQKHLDQQNQKQLSSDSLKKGIDRNELINDIVSLLEGKMNKSRFNILVQIDELFEVRYQKGNVKLQDLRISDESDSGKITLWNNQICDYKIGQTIRIDHVIYDNTKQEFKTTFHSKVNTNVPNKNLTGKHKKQNQKQLVIDGVEYQNFTYNQELKLIADIIKLQYNLQEELPYQYCRAMISEILIARLSQQCPQCTFTKLIQNNDVYECSGQNGIKHIVTLPKVYCYLKCKLTDFTASIEVVFGDDICQQYIPQNILNELYKNQMTVIPYFDCLMYKEFTFKLWLQKQKDQENKKIKIMKLFQTNYLKIIDSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFL9</accession>
    <name>A0A8S1NFL9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0740169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFL9"/>
    <dbReference type="OMA" id="NIQITMV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000566">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="11"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="211"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8085504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="377" mass="43618" checksum="803502A3FFAEDB23" modified="2022-10-12" version="1">MNSRDPPSKILLLIITYLPQSFPLTNDFLFETFKQYGEVKKILIFERGKTNKAFVEYHEVKQAILARRNMIGKSLTPQGGRLLIHYSRLKQLDLEVVDHTRGSEYHSDDEETLPEQKSALKSMTLPNSIPVSPPLIKPQSIEQKPNNGNQFNGIQNITEESPTKAIMETQLRQLERILDDDYEQEVAKIQLTQLDSNEMAIQELLNQQPSKFLKVSNLDERVTPRMLYNLFNRFGHLEALLLKKNIRQSILQFVNKENAIIAKELLNNVIFFGNELRIIFQNAYNALLQPNNAYEEYYQGSQTKFKIVPLSRVLQFSGISQLLEIQDMVKLVGKIQEIKLDTQYIQITMVDIYEALKVISVFSEYDYKGNKISLTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NIC1</accession>
    <name>A0A8S1NIC1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0890098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NIC1"/>
    <dbReference type="OMA" id="FFIRIRQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="460" mass="54761" checksum="B89224D4D2AB4664" modified="2022-10-12" version="1">MQIFLDHYKWNERRLDRCLEFIQEITDNEFIPSFFKNSVCRIDQLSDYLLKYQIETQTYELKDLIGRLGGFEDKLMFSKFQERYLRQPGIIQKKEADITFLRLLFEEIKLERQLDEQRFKVLKRWDYNVQVLFKKLSFGTNIVDQQKLKKFFIRIRQTITDDEIELLLKRIGRGDANIMTFEDFSQAIKTQRQRSQDKIEIPIMKISTVIKREPQHQSYMEKLRRRYKPLQTQQSVVIQKLDNKGCHTPRAKTPTATTRTTETKQARTRTRTPNQNTEPRSFTNESKELNTLMSVYLNQQRCLQNVMAQLEEVIILKSLISNDWQQEWSDLGVNTKNISKEFFLNILTYYDDRQPLQQKIKVSDLQSSVQIVANNLFELLSEQEQQLQKAKLQFLRDGTLNSLDRYFHKGYMDVLDVLQILEHSTTRSVSLQDAELMIAKFNKFDGKKTITRKALLNELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJV4</accession>
    <name>A0A8S1NJV4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0910003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJV4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="221" mass="25851" checksum="8575F8A4D28DF718" modified="2022-10-12" version="1">MTFTKSTFKLTLEAFDGQTQGVVYTKHLQQIQNSTFQGNSRYIIFMFSLLSGFLPPIHQQILQQTYIFTNFLFFRHFKRLGCLDIFQQNTLQKFSIVIQDFKFEKHQSSIMYINLHAQYNLLISTGESSISIRHNLDRFSIAPLSKRKASIQLIYDPYYHLSDNSLNSRQPLALLYLASLKAHHYPLYRQKGGKIKISNNRYLVNTLIHRALFAGLLYIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NL35</accession>
    <name>A0A8S1NL35_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">BAH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0910165</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NL35"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="8">
        <location>
            <begin position="80"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="206"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8092862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="30252" checksum="B55309081FAC82ED" modified="2022-10-12" version="1">MSVMHWTNVNNESPSKFNRQSSETLPPIRKRIQKRTEKSLKETTFKSLYKQNKLAEIVKKAQENNEEIYEEYDEIKIQGSILKINDNVIIKNGDHNVEDYVGTIQKICSVLEPQTNKLICMCQVQWFMKKSEILIHKPRARCWIGTQEIFSTKKNDFILAQTIIQKCKIVDCDEFVNLENSDLTTYYNRLEWDVENKKFTNMNEIQLYCLCQQPWNPELNYIQCDKCQKWYHFECVNLIEGCYDDKEYNCGYCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NMT3</accession>
    <name>A0A8S1NMT3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0930132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NMT3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="126" mass="15055" checksum="B9FF8ECB727E2D0D" modified="2022-10-12" version="1">MTEEAREKEVKNWRNRFQKLNQNQNKLYNEKLESNNKLASQELEIEIQKRKFTSNESELNLIRNNISQLNVEIERLRKSNDNLLKNNEGWREKQSRLQQQIPSMLQLIPSIISGNSFTKSEKPQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NP83</accession>
    <name>A0A8S1NP83_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome alpha-type subunits domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0880040</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NP83"/>
    <dbReference type="OMA" id="AGTHSEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000004">
        <property type="entry name" value="Proteasome subunit alpha type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="5">
        <location>
            <begin position="3"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8091383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="232" mass="26191" checksum="A7FC620CFB222513" modified="2022-10-12" version="1">MSYDTALTVFSPDGQLFQVEYAMEAVKRGLCCVGVRGKDAIILGVEKKATSKLQDVKTIKKVYQLDHNLCMTFSGLNADARILANQTRLQCQQYKIYYEDDPSVDYIAKFTSQQQQKFTQRGGARPYGISTLIAGFSNNKPKLFQTDPSGACSEWKATSLGKSAKQVKDFLEKHWREGLHEKDALLLTTKALMDVVESGNKNIELCVIRKNECWFLNENEVEELTKITAQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NRW6</accession>
    <name>A0A8S1NRW6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0910167</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NRW6"/>
    <dbReference type="OMA" id="TSGANYH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000808">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8092866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="203" mass="23244" checksum="807DA829AB47C534" modified="2022-10-12" version="1">MNRENQFKIVLLGDQGVGKTSISNVYVKKEFNSYENATSGANYHSQTCKIDNHQINFAIWDTAGQEKFKSLARLYYRDSNAALLVYDVTNYKSFQRVTDWINELNENSETALKFIVGNKIDQVDKEEVSYDVAKQLAENYGCFLRLVSAKEKNGIFELFESVGRELLKNEQDNQSPMTNQPAQTTNKPLVQQMPQAQQQDKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NSG9</accession>
    <name>A0A8S1NSG9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1000151</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NSG9"/>
    <dbReference type="OMA" id="MMLDAYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="242"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="93"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="251"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8096197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="312" mass="36569" checksum="DDA70AA54C5407B4" modified="2022-10-12" version="1">MGSCQHLCRQQHTLEQEITIQQKNKQRKESDKDSKTDHLCSQKGVLDDLVGEEFKSPKEIKQFTKRIQNFEISGDRKNRFMHDIEEEIKYIIQKNIQEEQENLKRIQQIYEQELELQRKEQIKKQKEQLDNQFSFQNSINQSNDQQSVNPFQPKSTSQFAPKRNDNDTVSQKSQISKTPTPPCKDKSISSQSDIMRKSALMKIVQVREELASNNASKGSSKKKMMLDAYEKMFSEFFSADEKGSSSYSKTQKSHKSILKNRSLTHRSFSGSKSIKSTHTAVRKSKKVRFSRDTNFSYERKGIEKKKKSFWDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUP1</accession>
    <name>A0A8S1NUP1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0860193</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NUP1"/>
    <dbReference type="OMA" id="KEIYGWR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015149">
        <property type="term" value="F:hexose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF8">
        <property type="entry name" value="FACILITATED GLUCOSE TRANSPORTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035944843" description="Major facilitator superfamily (MFS) profile domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="7"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8090754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="461" mass="52485" checksum="E23F2FB3BCF2D21B" modified="2022-10-12" version="1">MNKLLLIILNCCLGSLFMGFCLGQMNVISQEVYNVYKIDDAVTKGLMQSLLTIGGGVGSISASILMGIFSRRRSLQITDLFGIMAIMIAFIDQSKYALLTSRFFVGVVLGLNGVLVPVYINEMSPKEKAGFMGTMNQLFITLGILFSFLMSYFQDISLPIPFYKIMLYLPIIPCIVRSSALSTVFKYETPVYCVKYNLNQQLHRVLEMIYKEKGDKMYQQFQQQQKSTEQRITFNQLFSSKYRFRLFIGISLASLQQLGGINGIMFYSSSIFDQVTGQASQKVFYLNLIVGFIGVFTALLATIIIEQFGRKPILKYGTLWCFISLLMLTFVMSNNIGNTTFGQYSIVICIFSYLFGFGFSQGPLLFIYLTEILPDLGVSASGLMNWMSGGLVAQMFPIIASYDISYCFALFSIFNFIAYLIIQYKVIETKGLDRETVDKYFENQKFDYIPYQEAYQEERFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NV80</accession>
    <name>A0A8S1NV80_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine rich repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0980083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NV80"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="30504" checksum="671652A7A631D039" modified="2022-10-12" version="1">MDSNIVTLIEQSNKYNSSKTNFFKTKLFVSNNHSPNREYSKHVAQTETVQYNRYQSKEVNSGRNSKKISFNTYLLNLAKQNIDMFPKIKCDFANIQMVDLQDNRFRLFPKELYQMKKVQIINLNSNFIKVLPEELFDNFPSIINFSISNNLVVMLPKKISHWQSHLEILELSKNRLQKLYPLITLINLRSLFIQSNEFQYIPIEFKNLKKLNELGLDWFKYLNPPIDPIVNRPYIEKLFQSLEEKQLKDVEDPSQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYB3</accession>
    <name>A0A8S1NYB3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0900145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYB3"/>
    <dbReference type="OMA" id="KNWIIAS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="623" mass="74060" checksum="7285D473FD261275" modified="2022-10-12" version="1">MNLITLNFEVSQIEKEFRESKQKLFSTILVYQKWIFGLINFLSFVSCLISKNWIIASISLGTLILILISIKVHKKYPYVYEIAIIVYILLINAYIALEYQFASIDWQYTEIFFISFTFASLLNLMNFIKRALLLLITVTFHIIFGIRHQDINADTYIRYLLYVILYVQFMYQFERLLRIIYLDYQKCQQFLNKISKFKDVQSYSVKYDERKSLILIEKNNNKRILKQDQEEFLKMITNMQLSMQNQKSRVAQIISVDNLFSPKKQSLKQLLVYYTQQSNTDINDTPSKIREDIQIYGFYQQKVFALSIFRSIDVQPITVLLMKESKSESQEEELRLRMRNNQKQLNYIQQIFDRQIKKSLIYFRWIKNFANKQTDGYLINKSLKMINYSLIRGYTDFLNLETFSLSKMLCPGIKQFDLILLIKDVFAFYDGYNKQKPESMSYIQQYEIINNLSSNCILSDFKYIKLLLFNLIHYTSEKSQTIKVILDEVTTEFSQTPIIRIKIHYQAPNLTKAQLHSLPILNPQSLEELKHNSTVPLDLDLAVSLILIRRLGPFDRMKLVQQKRKCNYLEFYIFQQLMEDYLLIPIKSMKPSDKIIVQEYSKIANSFSTSIQIESVRESFQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P0W7</accession>
    <name>A0A8S1P0W7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein farnesyltransferase subunit beta</fullName>
            <shortName evidence="3">FTase-beta</shortName>
            <ecNumber evidence="3">2.5.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1030073</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins. The beta subunit is responsible for peptide-binding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-cysteinyl-[protein] + (2E,6E)-farnesyl diphosphate = S-(2E,6E)-farnesyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11535"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:86019"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.58" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P0W7"/>
    <dbReference type="OMA" id="WCIYWIL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005965">
        <property type="term" value="C:protein farnesyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004660">
        <property type="term" value="F:protein farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097354">
        <property type="term" value="P:prenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02893">
        <property type="entry name" value="FTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026872">
        <property type="entry name" value="FTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF6">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="4">
        <location>
            <begin position="54"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8097141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="367" mass="42046" checksum="C5D9E623524CABF3" modified="2022-10-12" version="1">MKQVNRTQNEQNLVHSAIAFIRKQYECIESEEVPQELLDNKYYKEYEPLDKLELLTDQHIKYCTKRLLNIGYSIIYLDVGQPWCIYWPLNALSILQEDVSKYEHKILQYLEKCKIGGFGGGPYQYEHLAPTYSSLLSLFILASPASLGMIDRKGLEKFFWSIQDPNEKGSYLMHVNGEADIRAVYIVVIMVVILKLDPKLLDGCAEYIASCQTYEGGIGAVRYSEAHGGYAFCGYAALVCMGKAHYIDQEKLLNWLVNRQMEKEGGFNGRTNKVVDACYSFWQGAIFNLLQLSGYLNEQLMNVYELKTYIHMCQNPTGGIFDKPSKSPDTYHTCYGLSGYSLTDSNFQNPIYNVPNKCIDFVQKFFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P0Z4</accession>
    <name>A0A8S1P0Z4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1000120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P0Z4"/>
    <dbReference type="OMA" id="YWIISSH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019776">
        <property type="entry name" value="Flagellar_basal_body_rod_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00588">
        <property type="entry name" value="FLAGELLA_BB_ROD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="916"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="942"/>
            <end position="964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="964" mass="112433" checksum="9CB8E6F2E9C2C9EA" modified="2022-10-12" version="1">MIGNIIQAIGELLNCKVDFLCTAQLTSDKKYFAVVASDRLLFIASKLEKVEMSLEYGKIKGIVISTRVPTMFQIHTDIIIDVYALERKQLIQALRYSWMTDFMHKNFQFKQLPMFKDDIKSNIQDKQPVLPNLMFTKQGKQMFRNKNYTFSLPDEYEATYQDGVYVHKESQQCKIVVQITDPMPTQLLSQLGEKANLQYYSEMFLEIVLKGINSKDDSVKQGTNQKSQDSDEEGKNQEEDDQSKQKEETLDYWIISSHNYKKRSNLMNDISEWFGWELHARTQVQDIFIIILRRIHIPPLFETYQEILFVLYGQEQNAKEQVLVNDRDLLKLTLEQDLEEHAFKNVLRNAIDSLQCKMFIFEKQYQIFIQLSADRLLYNRQTLNFIYNKFKIFPQFMSYALKYLKLLFQRLVLYDKERFLILDKELTQKYGFILNNLKDYMTDGIQIEENLQALFDAFEQASFVRDKSGNEEERKFVWQQKLSNYLGICFDGLLMGSAFSFKDLVPVLQAKVSMELKTIILELFDYCLYAVPKKECRPIKKGNFIKKLEELASNKYQYNVNIAKSLIEANFFSQEVLLQERSGLYSSLICYFLQSDNVDLIVAICRHIINFQAEIKHTTGLTEAMITAFKPISVELIRLYRSHNRTIATLACASLFNLCTNSREFKYIILNDDGAALLVSKLVTKDNFLLHFALKLIYCVMSITQNIEKLLAANLTDYLYKILEGPQIKGCQYDARCLITTCQILSVCLVSEVELRNIDHLLILLYNMVTTQADQYFLDQEESVNLKSEALYVMSKMCHKSAEVKKKINQECVPYLLKLMEKLTYHQVQEKIIMLLCILIKENKEWALEWLQMQIKLQDILTTYHNNDIQSSNYLLSQIKFIDDEAKKVKNEEMKRSKLAPTASVIAEQMMEQLQQQESTIQDNKKSMVSEARSSMIEDSRITASNINNKSTINPSQFKKSSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7W3</accession>
    <name>A0A8S1P7W3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arylamine N-acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1090031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7W3"/>
    <dbReference type="OMA" id="HHIDENW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2140.20:FF:000008">
        <property type="entry name" value="Arylamine N-acetyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF0">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8099207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="34413" checksum="D8825302B0E741C5" modified="2022-10-12" version="1">MQQLISQEQYQTYLKGIGLPKDIKLSVSVETLYLIANRQLIYNFYQNTELHLKELKPISLEFVDILQRLCVQKRGGLCYEHELLLYFVLKYLGFHVELIRCQVREPNSPYNPDLPSTHAFLNVQIGDETYLVDPGFGTRSYRFPIKVNFQDLTQTHELFAQEHYRIQENETHYQFQHHIDENWVTYYDFEKPLKYASVEDIHKDYLNLFTCKQFLGVRDSRFVVCRNTEYGRDQYLWFRLEKEFTAFKKVLKFNEVSKIEFKSYDELKEDVKKEVGFELPDYELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHP3</accession>
    <name>A0A8S1PHP3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1190050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHP3"/>
    <dbReference type="OMA" id="YPNSSSC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="154" mass="17970" checksum="FCC0CC4E5612DA82" modified="2022-10-12" version="1">MFKILPRHIILNILQYLSIQDILRMRRVNRQLWMIIQAHYQMSLLQCSSQIDLSKYKVIGVMLQIVAKDSGMSKHKPMQNYLMKLHSGRIELSYYDSAYQKIGQDQIVYVVQKGKEKEIIRNLEIIGVSPQSISVYPNSSSCKILSILCMILYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJI9</accession>
    <name>A0A8S1PJI9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1190174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJI9"/>
    <dbReference type="OMA" id="SNEHMTE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="102" mass="11959" checksum="C42C43920A54B03B" modified="2022-10-12" version="1">MGQSCCNKQTIEVSNEQMTEWCQNIEGVKFCENLDDIFNQHDCNSNQARILSSQIIFTFQQQLPPTLSQESINYDKMNSLQKENLDEESPSFKAQQLRFFRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PL26</accession>
    <name>A0A8S1PL26_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1220109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PL26"/>
    <dbReference type="OMA" id="ILDMSHP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004483">
        <property type="term" value="F:methyltransferase cap1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20760">
        <property type="entry name" value="capping_2-OMTase_Mimiviridae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000176">
        <property type="entry name" value="mRNA_MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025804">
        <property type="entry name" value="Pox/kineto_cap_MeTfrase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01358">
        <property type="entry name" value="PARP_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51612">
        <property type="entry name" value="SAM_MT_2O_PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="449" mass="53137" checksum="6A07D918F6F7DD93" modified="2022-10-12" version="1">MSMRIVQSTKKILDMSHPSQTMLNGQRPSMSLMPKEKVIIGLNSKQELNSKQEKKTNKIFIKLEEHINNSIVEQKVEQNNPIVEQNYPIQCPIIQNPKEDQQKNKETKISMEITHTHFELKVPKIIVPKSLEIPKTLEIPKILLPSQIKIEQEDKSMTYNQTNLLKQQLIQIKKFNEPIQLLLQDNDKRKKYYSRAQREKEDVIHWGQRKLLISEIWFLTKYGCLSRNIIYAGAAPGFHISLLSDLFQNHKFYLFDPNDFQVKQGPKITIYQRCFTNEEAQKFKELFQNDYLFISDIRTANFKSMTPLENEQAVLRDNQLQMEWVKILKPQKAMLKFRCAYPTEINEPTEFFEGEIYIQAWAPASSTETRLIPYNYVNTIFYDNVKYEEQMFYHNDVVRKKQKGSLSWDDRAETEVLIEYLRNSGINENQIIKQVNIIKDQITKKLNYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PPG5</accession>
    <name>A0A8S1PPG5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1240102</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NINTFIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="955"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1059"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1156"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1222"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1247"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1279"/>
            <end position="1300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8104629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1338" mass="155198" checksum="CC980A28AC332646" modified="2022-10-12" version="1">MITNFLFIDNFQKNRNGISLQVILLLAMIINGLNRKIVYSKFFSTFTTADNDWPSVGYNTYNYGVDVCGTKNIIFVDNLSSLSNSLLYQTFVLEPHYQVSLSFKFWRIDVWSSKQFTTYIDHQIKYQNSFSDSDTTTDICKDATNDQVVDISFTFQHNTPTLMVIFKGDGYRWGLSNIEIAIDECSTGCDSCSQSQCLDQTLVPISIFWTTQEALLKQACGGYEYQQTLGNFMEIQITLDNHNVINSSMKFHIFNADNPTLTVKVDNQLVTTTKQLSGRVQNAGYFCFHNQILELQIIDLDHTNPTIKFRIDVVLIRFDPTSVMPFFGIKEFQLFIKTKIKQTDPASLGLPFEGCLMSLPELFEGCSFCVRSSCLHCQEGWEYNEQDLKCQPICGDQQIVQNEECDDGNQIPYDGCYRCQYSCSQFCQQCLKGKCIQCVQSRQLINGQCFNVCNQVLNYYDQLNNGCFFHNQNFQMNGYYQHTFLNNNNLKYSLDEFLDCDILKYGIMGYYYNQCNIRQILYCKIQLWNICLECQDGYQLEFNKQNCVSKCNDNYKLDFEICDDDNNVQFDGCYKCESSCQLECLNCVNQKCLLCKEGWYLIDDQCLPICGDGITVPSEQCDDGNQEFGDGCFECQAECSYCKICNYNYKCQVCQEHFQLVSQICQPICGDQYIESGLEECDDGNEVQYDGCYNCQLECYPGCQNCQFGKCNDICSIDEINIDGKCIKFNLNEENNSLSYSNCKVGCQDCISDQQCIKCKKNYELLNGICHLIECGNGIRENQEECDDGNILNNDGCSKNCLIENKWNCLSKDSQTNTCYSQTQINLEYLNLTRQNQFIQLSYSKKVRLKFNSDNNNFLSQNSLKIIGLQEDQYLITCYPVIAIDQQEFKSILYQFEIQFFEQILNQFNFTVFINETLLDVNDIPILPSNVSIQLKVPKLLTSTQLTFSKTFRKVGYSMMISLSCSSVLMLLLGEFAQTIALLDILQYQQYLKYLNVEYPYNVYIYFESSNFITFQPILNYLNFFELYESIVQKHFQQSIGKFREYELNADFLTNIDSLLFQLSCGMILIIILYAYEKSISKHIPLLKIVSYFAKVKYQFIKSIFIYLYKYKRKNLEIIKIININTFIQFFYANSWDLMFKIGLFLISNTESENRQRASILICLSYLGIMIKIFLFDTQRINGRAKISLIKKVQYEKLTLFKKFSFHLLLTSMQYHPITQCILIPFFITQYICLIFGLKLKTQKLEIMTILSLEISIILCILLNILYCQEFENYLSLNQQIYIGFAQIGLLILSLLGPLIQTGFECYCKMKNFINSRKLKNPDQQMTIITTNIFVQAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PR08</accession>
    <name>A0A8S1PR08_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1270073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PR08"/>
    <dbReference type="OMA" id="QSQIRNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="187" mass="22382" checksum="0FD7BE9D21D343CB" modified="2022-10-12" version="1">MDDQIQTIRQLQLTIQLASNENKELREKWLLLRSQYDPELDTKINNLEKTILFQQQQIAQLQQQKLNSSNIKDLDNEDEQYQIQQLNLKINELNQQLIKYQENVFQIQSQIRNCSEELSDLQQTLHQHKTDNTIFKKKAQQMEQLLSKVRGQEPQSICYINEIDRLTIKLQQLQKNSQIKSRLTYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSA9</accession>
    <name>A0A8S1PSA9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1280163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSA9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36649">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36649:SF28">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="424" mass="49994" checksum="7D74D8DD1E40B7F9" modified="2022-10-12" version="1">MNQYSAKQHLQSIIKQTQIQEQILTQSTQKQIEMLNQEIYQLDNNNNLTNNQIQYYKNQYQQQLIQKLKDLGQAKSIPKIKQNNEANILIYVLQESLLRDQNRELLITSKQYSFGFDSIIGAYGSDQSCLKVRYKNLDFDKIMNDNLLLEEHLLEFQQKLSISLNISIDQIEILGVSKERFEISFQIIRSNHEEIQQQINYSPNAQKFLNEYCHGQIEHVAYFDKARAVVGDLEKGCSGVALSFEDFNPQFNMCWDNFHEKEQRGPPYHRYDYYFPRGCYGLGLKIKNYKNDDWIKMDGNVNEWRILYHGTKNCAVNSNIENNQVSGSRQVYAADQCMDEFGNEVRVGTGIYFSDQYTVCIEDGFAEYIQLGNKWFALIFMSRVNPKKIRQSERMKKKNYFVVNNSEDVRQYRILFHQKKDINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PU52</accession>
    <name>A0A8S1PU52_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1320012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8106847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PU52"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8106847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="25035" checksum="BF068238180A1E16" modified="2022-10-12" version="1">MGYSNTKIEEPFSKKLRSKAIINFNKLNIKTMELQINKERHFYGNLTMLNQILKHYLKFWILIKLKILLNGWLFWILLNKMVILKKYQVLHELREGKAWVQFKRVDRFIKIDEQQRGSRVKSIIQEEKKRLSVFQPARLQKCKKCILKDKSYFKSKNNNNDYINTYQKILLQILQFLFLQISCKLKYIYTFVFIFQIKVSNMPNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PVP6</accession>
    <name>A0A8S1PVP6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1310139</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PVP6"/>
    <dbReference type="OMA" id="PVRMYCL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000245">
        <property type="entry name" value="Methyltransferase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008576">
        <property type="entry name" value="MeTrfase_NTM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753">
        <property type="entry name" value="AD-003 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753:SF0">
        <property type="entry name" value="ALPHA N-TERMINAL PROTEIN METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05891">
        <property type="entry name" value="Methyltransf_PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016958">
        <property type="entry name" value="DUF858_MeTrfase_lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8106738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="236" mass="27634" checksum="8C972157263D1F10" modified="2022-10-12" version="1">MNSESGNDDKGKFFKNIQALWDSQLKDSKEKWYSLGDQYWRKIEPTLKGVLGGLDFLNKIDIEESNKLLTMLYKNPLQDLRVLDCGAGIGRVSKELLIKWFKKVDLVEQNPIYVEKAKEEIGDKICEYYCAGLQSFEFQYKYDCIWIQWVASHLTDDDLIMFLKKCKLNLNENGYIILKENITKQGFSYDTEDHSVIRSDQMFKQIFQKGGLELKFSGLQPNFPKDLYLVNQYVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5W0</accession>
    <name>A0A8S1Q5W0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COMM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1460054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8111016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5W0"/>
    <dbReference type="OMA" id="GVLIMKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="1">
        <location>
            <begin position="97"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="167" mass="20085" checksum="566BED2FCA2AF828" modified="2022-10-12" version="1">MKPIFRGVLIMKYTDNQEEVTLDQLKEYLKEQGNYNEETFSQNVDIFQQLIDDLIQNDSEQTYEQYGISSQELHQTWLTEKDRVLSYYIKKRTQGQKIEKFQWSVNNQFYSKKAKNINKPVCIMNLKSKDVVNKTYKFHTFEINEEDLNYIEDQMKKIEQTINALLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q6M1</accession>
    <name>A0A8S1Q6M1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1460095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8111098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q6M1"/>
    <dbReference type="OMA" id="ACFEYDD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013887">
        <property type="entry name" value="UPF0592"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35397:SF1">
        <property type="entry name" value="ARMADILLO-LIKE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35397">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08578">
        <property type="entry name" value="DUF1765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="996" mass="117925" checksum="E707519F548FFB53" modified="2022-10-12" version="1">MGICSSRSRENLLTDIDSVPLLTRGQLGFDVIIENIKVKMDDMQSCKVKMAISTFSSFETEVEQESQGQFYFKHIHKFQLKTTEEQLKQQFVVVELQNEKGEQISTFKLNLYQIATGPFHIDYEFSQVGSRKHGKVSLDFKMAQILIVRFIPKLIDFTLKEPLNQNEYCFQLRMLTSQLQFHSEYSPVFQNHIFQKTQQLQSNKQIIWNDSKVEMEVEVPLIEITSSSIQISLWHISKDGKKEIECEAYVNLNYALTQKAECITDKGIKYYRIDWKQNNRRVWNHGVQEGMLNYHLEIILPFHLKQQIVGVRTDKGVSQGTRVINQTKASIREIQQLSSAGQGLLDTQYIILSKSEPDQQLITQQNDFINQIIENLKLSHKQSMVCFEYKTQQDLFNSQKLLIDLAIRLLETADQQQEQLREQYYTILKLLMNRGELSLSQLGFSDQTQKTNEKLLKFKMDIGLSYQIFLQKGLSTTLEKLKQKSLAQNERSFVENFFANAYFRVPEFRNKIITSVQRADDEQIPEWRSLTKSSGGISNLEFNDIFDWNKHFYEYLMKDPKYYANNHKMNETINQTMWQKRLGKRGIAFFLFLKEWCDLLNTYIVNSKNVPYHQLPGYFQLVKAFFLELKSREISNYPEALKIALCSLLRNTNLLNTIIVILFNKVNLYNSDNVVHCLDLLNKCFQKISQFNLCMPSFLDQKYFLKGIRIILSQSEHAQSIAKCLELIYTNYLLFPVDLKKELTDMIFENLAIKFFIHWSYNVRVIFHCFLIYRIFHLHKPIKDREFNEEDLIEKYQEQLKPKKQHSYFDQRNSSKQIISEYIYLKYIRLMQQIEEGKHLSKQKISYFNLENNTRVEELKYKLKKLQWRIQNREKDDSIVQHSFKIIPDNQCDDTVLPLQTTEQNQEKQVMNKGQLPKQKIILSEAQLKYLCKACFEYDDQIIQYQKWRQTNLPDNYNTLTEEQILPVIESFVVPVVRILELKDENEGNLIQKDDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8W4</accession>
    <name>A0A8S1Q8W4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1490147</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8112002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8W4"/>
    <dbReference type="OMA" id="GPLNQIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="2">
        <location>
            <begin position="8"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="379"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8112002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="474" mass="55271" checksum="EE92215BFAA72D51" modified="2022-10-12" version="1">MNKKHKQIVLIDFIGCGKTTLFNKISNTDCKVKQGGESVTRQVFLKESSNGQGFRVLDTPGFGSSNNKIEHAVGVLNALTEGPLNQIFLVVKWERLGLMRNYIKIMVQIFMRYRNLVTLAVTHMDTADKETLEKDKLEFVNGMKTFGLNSFVYFSKNDTSKQICQDIDKILIKTELQNVELTETEFIVISISQNIQMTQKWIQKQPKDRQQLILTKFLRLQLNLLENLIINMRICQIQCIVQHQKLRKQLKNKYLNLKQKITIFKLYEQCSDPVTAYLVDFALKKNQWNKQIIFLNTCFFIQLTYFQLMLQYYKMKNISLISQRPAQIVVQLGQKQQVVKEKKNCGNFPEEDEYFQNYKQTQKYVFEISEKGVKFQERTNTNQQNASQTSQKTTKISKDGKQGIVRKGCGAPLTWSEIPPLNEFQIKELIDPGIVDYLAYEIPKEELKKRTKVAQQSLKQRVEQAKDQLKVVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9F7</accession>
    <name>A0A8S1Q9F7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1480066</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9F7"/>
    <dbReference type="OMA" id="EQIIPQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045862">
        <property type="term" value="P:positive regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058209">
        <property type="entry name" value="TPR_BSK1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25575">
        <property type="entry name" value="TPR_BSK1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="98"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="8">
        <location>
            <begin position="252"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8111614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="336" mass="39247" checksum="046D9C56123B90C3" modified="2022-10-12" version="1">MGQAHSNETYQFMNYPPEDIFPQQQPQQQRKVFKAAKKPTAQSTSQKNINITSTPQFTDNKQQSLEEKIKGNNFFSQKNYQKAIECYTQAINLYGTDSIYYSNRAVAYRLLNRYQEAKQDAEQAIKIDQTNARAYFIYGTIILLEVQKSPQISEQLIKQAHRGLQYLDQAYEYIKDNADQQKNKLKVLINQNLGKGKRMVFLIAQEMDRRNVQSLKSILAELAQSRKVKLDWNYLEKQTQKKMLQEIPIPQSFICVISYEIMNEPILFNTGQTYEKNGIFQQFTQNGCTDPITREQIIPQTARLNLQLLQGISELKKKYGWIGIEQEEDYKAIKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCX0</accession>
    <name>A0A8S1QCX0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1520141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCX0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8112787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="67" mass="7984" checksum="57E7F0A20DFBD2BB" modified="2022-10-12" version="1">MIAQVSFELSKILHFQIKLFRIKYLQNHYYYNIIIGFILGSCLVINIRSYARVVTDQFAYVFGILLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDD3</accession>
    <name>A0A8S1QDD3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Spindle assembly abnormal protein 6 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1560074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDD3"/>
    <dbReference type="OMA" id="ECKMRAT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032396">
        <property type="entry name" value="SAS-6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34230">
        <property type="entry name" value="ASSEMBLY ABNORMAL PROTEIN 6, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34230:SF2">
        <property type="entry name" value="SPINDLE ASSEMBLY ABNORMAL PROTEIN 6 N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16531">
        <property type="entry name" value="SAS-6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spindle assembly abnormal protein 6 N-terminal" evidence="1">
        <location>
            <begin position="21"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="180" mass="21206" checksum="0881581924CAD115" modified="2022-10-12" version="1">MDIRSYMIAFQTQIFDSKIGSQKSRNQVNQNLYVQEFYNQGIDSNPQSIKIEISSEKDLFFLFHHEATINSFTQIKALQNLQMPFKDYAQITIATLNKVEQNFNGYGAIFQVMLDGSARLEINQTTEFRSVHMIHFDFHQVEESFLRQTIVFKYNQQRKEIKYLTQSLNECKMRATGTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDG4</accession>
    <name>A0A8S1QDG4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone-binding protein RBBP4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1540067</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDG4"/>
    <dbReference type="OMA" id="RHWKPNA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051972">
        <property type="entry name" value="Glutamate-rich_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903:SF1">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="5">
        <location>
            <begin position="126"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="385"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="430"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="26"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="32"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8113164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="553" mass="64490" checksum="8BB9AB6A9AD2E442" modified="2022-10-12" version="1">MQSRSQRRRRILKQCIQKKSKIEDNAHVTQEIDSEYEDVSEMEEDEHIQYEDEFDDEYESEEGEINIDSEDEEILEKEEQSQEQSQQTQQQQQPIQQQSKLKRKKKGMEQENQENQKRVWFYDEAENLDFDNRAYNMLHRVTTEWPCLSCDFVLTEEEQQQYKNKEYQKMNKYPYSVYLAAGTQAAQPTKNQIYLLKLSKMHKTKYDDDEASQSEEDSEDDNLSNDEEGQVHLSSVTGLKCGVNRIKTMNGQAIAAYWNENGDVNILDLNPLYKKLQSNQQSQFNLSQLHHKVFKNQHEGFALDWSRLKVGDLLSGSVDGKIYLYQLNNNDWIRENKAYEYHKGSVEDLQFSPIESFVFASCSTDGTLCIVDTREGKHKQAQILVKAHNCDVNVISWNQVSATLVATGADDGCFKIWDLKYPKNDAISEIQFHNKAITSIQFQPNSDSSIAVSSEDHKLSIWDFAVENENNNEDDVPDQLMFVHQGQKDLKELKYHPVYYEMIVSTSANGFNVFKPTLDEEEKKSEQSEEDQDQIPVIKEEDLNKYFQQMSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QEF3</accession>
    <name>A0A8S1QEF3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1540091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QEF3"/>
    <dbReference type="OMA" id="KTIVFHW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="150" mass="17638" checksum="BC996BD05B81DB62" modified="2022-10-12" version="1">MNISESIHEENLQTKGGSGILLTPEKKDLRPRKNLIPGMLQEQKRTYIKVPKESKEQLYQLVFKEGFKIKEAARKLLIKYATAKTIVFHLRKKCQKQKKQENKFSRYIQLQENIIVKLKIISIIQKKVMSCVEYSIQHPSNGQCYFKLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFM6</accession>
    <name>A0A8S1QFM6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1570114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000162">
        <property type="protein sequence ID" value="CAD8114034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFM6"/>
    <dbReference type="OMA" id="FIAVITH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="560"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="614" mass="73605" checksum="75F7F6BEB5189DA3" modified="2022-10-12" version="1">MHYSLIALMGNDQQLKDSIYQLYSGMASQDQQIPIPYSYSVLNTIQQHKSIQYNILNTPSTIKKDELISMWNNMIQICKLENFENCIIFYCFTDVQNDIIPLLNDVHIEMGCQINQNNILLLTQYQTLNDYVKKQEKNNNYKIILLDRYQLKNIFPLLKEKKQLNVTNIKPRIYQGNNLKEIADNKFNIIFTKWKEIITKSKTQAEFLKNILLEQSISQISDKLQVESQSQVQIFNLAVNIRQMLEERKKDFIDNLMPKSLKFSSFRYCSNQKCKWLFSYNSSNQLEHLHQIEIHLDNPSFQNYYCLACNNKICYITFSQIINDCFQEFSFRIINEVTQNSQQKDSTEILEKILQIMLLSNEEEVKTTIFNIIQQNYSTNERQSSQFVQSSIINNKYRFHNAPLFNFIQDSENTITQFKDYFYKNEIHFFLILVKFERTDMMIQKITNMIKKFHRLNRQRMVLIINQCQYDEEQFAEIKQKFAHFSFKNILFLHKSDCTEDICKNIFQNLENISLEKFDFTDSVFEQENDNSFQQLQKQNLINLSRQIDAEQQNNFRIQLEQDKEYLEKLQMEIKILQETLQQKKTEQNNLLYKYQQDSQKLKAWEKQGILVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QH19</accession>
    <name>A0A8S1QH19_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1570031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000162">
        <property type="protein sequence ID" value="CAD8113870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QH19"/>
    <dbReference type="OMA" id="YKSSTEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="12196" checksum="D565B2E34234DCB3" modified="2022-10-12" version="1">MKQNSQQTRTYQKVSNIKKKTLMNMVFILGFKIKHAAKQLNIKYAAAKTIIVCHRNNVIKQKLEFKSSSECKIVSINSKKCKITIITRVGGDAVSQISFEYSQKQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QIJ4</accession>
    <name>A0A8S1QIJ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1590081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000164">
        <property type="protein sequence ID" value="CAD8114370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QIJ4"/>
    <dbReference type="OMA" id="THKKQVN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="141"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="189" mass="22106" checksum="07D944E19D488C17" modified="2022-10-12" version="1">MATHKKQVNSLYNVRFFPDKSNSNNVVKARSPVIQPTHRLQQQRMATMNVNQQNQEAPLKKPQLSRCGSMGMQTQLPYSDSLTKLQEIEKDIQTTEVETISIDKLQKWRIALIEQCESMEDQLGKKEVNVHQYAFKVVPHTQMLEKRIIEEIQNREKLEAQTSQMIKYQDQEINQLLQKIQALQESLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJ35</accession>
    <name>A0A8S1QJ35_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1650104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJ35"/>
    <dbReference type="OMA" id="LYNDPPL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000267">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="95"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="208"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="591"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8115682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="636" mass="74578" checksum="C399CF06510DE291" modified="2022-10-12" version="1">MNKFIRKSSLNTQALKELENKKLKQYILQMEQDPSLYETLTQEQIFGILCIILTKSSYHRTQSEIEILKKATIHIDYFQKLIEKEQGPLLWERCLRKMSYTYLSYGQTLFKEGDVGTTFYIILQGRVSIHKRLLVQDEFQDKELIQLQDGQGFGELALENNEPRSASVKAILPTHLAVLEAEDYMAIKKTVINQQRQMYFEEFAKLSIFRDWKFASIKSLFDVIKQIKYGLNHAIYREGDPSNEVYFIQTGQFKVVKTLRIPQQNDENNSQDDLQNLKDRFAYSKPCLSNSDKIDMLYQKKKYGNLVIRDNKSIMTLKFVGAGEMFGELEILKQPDLCRQFSYISTFESNTIYYVSKRDFLRVLYNDPPLSQSLNSLNDDKLKQALAQIRAYEKNFIDQTEKQIILERTQIKEQLNPKLITDEDLQTTVLVRNKTHVSKISSKKQQIDKKIRELTLTLQPPQSDMNYSTLLTQLYTSEKHRRQRTEQQIMQISKKGPRNIKTSFDQRLKSEIPIHDSPKQNVYICKVLSKLFSSQPTIKTKMNQEEEKTFKLQQKFNLKEIKKSNDYIQDNSQSLLKKQATESSLTKRIHSTTSRQQTSPTQSKFAQTDKICSNSFKQHLPFFLHNKYFGLIPKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QK68</accession>
    <name>A0A8S1QK68_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1620097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8115077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QK68"/>
    <dbReference type="OMA" id="HEQMFEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="1">
        <location>
            <begin position="249"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8115077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="395" mass="46179" checksum="040EC376EE56C833" modified="2022-10-12" version="1">MQSPTSSINKLRNSLTSFYVKSSCSNKTSPIKEDPTILQNLLRMQQKEIDRLEHQNKNLQVLTQHQCSILYQERTLRTNSTKLQIHADLPQPLPNARNARRSTIKNVLQAMKPPPRKIVVSNKLQMTNNECFKLSSILEATNTQAKQLYHDDSSIQLVKEILKDEDSFIDIITTYPPQQVSIMYDKIKKLINEHEQMFEMILKFKQIIDNSFNLQNIKLQLEALNQLSQQCKNILECKNAQIIINNDETSKSYIQHVYNNKQILNLKNAKFDERFKNENFNQLLAAPILDNNRCLGVIVCQDKSINFSNEDEILIQYICRQAQYILNNQISNNKIEIQLQKYQHALKSLLLLQSIDDLDTKLHFSQQRLCEIMNKYYMGSYSQQLDSFYQQLLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QMA7</accession>
    <name>A0A8S1QMA7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1900001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000199">
        <property type="protein sequence ID" value="CAD8117148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QMA7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="532"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="789" mass="93490" checksum="178E3CAB8C4AA432" modified="2022-10-12" version="1">MLALVNTITSSLRGGGCGSFKMNPRTYGNLQSDIQDIQIFITKFNSFVEIICAKADVAINSIESQEIMIAIQWFIFQEENIYKLNQNPQNIMKSYNLIVEGIRKLLKSCLIYIRTDSFKCLHILQITASLSKVIFSFHVMNSDRIMNFDLQKEFLDISDELKQQIELEKNDLIQIQIEVYLFLTKTSFEMAPNNNHERQDIVKGFVSGIFESLIQLKPNAQLLESLFMAACKIHNIHTIQKNRKQYEVYFQIDMLQWEIIRFFRNEKLLILKEILLQIQGIYENLVKNSKNWKNHFLWIQMIGKILIYNPLLTKQRLNQLMNSNDFGANFGQMWKEYQKKGLIIQTNHSNDQAVILLSQLQNNKLIQLDRNMLEDSFKGWEDFLIIKQYLIGELNENTPYTIRSYLKSKLDLFRKDYQKNEKILTIEKMKMLLSFIIPTKLINLIKQNNVNLGEVIKVQKQFRKNEWVNKRLLQSTVDSQLKKIICNLDEYLQNTQKILKIFNLNQIYNNCSAKQFQQPFEFNILFQLKYLMQLLEEILLQAIQEIKNKINQLCKQDGLNDEIIILQQQKESIQEQLKNLNLKDNQFSFVNKLYQQLLDLLIATPDQKGFLKKAKSMLKLIEDSKKQILRLEEISLKKCKFIESLLNHLYIEQDHMVDKIQKDVKKSILSFKAILISQYEIQSVQEFGEIQQQISNILSFEFVYKLRLKNLKLKLMLTAMKESFQNIFLAQLEEFQQLQQVINLDEFLLNVIINYPQKFYCCNSGLQKRDLKKNYKYFSQLPQRTYQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QMQ6</accession>
    <name>A0A8S1QMQ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1660057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000175">
        <property type="protein sequence ID" value="CAD8115820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QMQ6"/>
    <dbReference type="OMA" id="FKHIRIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8115820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="276" mass="32229" checksum="B38E46587830BE98" modified="2022-10-12" version="1">MSQTNQNNEKIQINDEDQITFLENELQLKNELLETKNQVIFKLHSQIEDLHKLINDQKDSARNCNDSDSDISNESDNQKINNLLQQIQTDADIQHHLTKQMLQYSEETRNLEQQIRELRDQNQDLKEELDKLTIQITKLKEQQAEMEENNAKEIDIINEDYQDKLSKQNMIIDDLQQQINSLSISPMGLPQISMPQTMKYISQQQFQTVGSDSQQSKGSNCKTKEEKQQLMRNIKRMTTMTAGLMSNKQDYTTLGVLGKIDQYKSQQKLESFNKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNC1</accession>
    <name>A0A8S1QNC1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1690057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000178">
        <property type="protein sequence ID" value="CAD8116155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNC1"/>
    <dbReference type="OMA" id="GRWDIFE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1082" mass="127492" checksum="441F011E8E6BB362" modified="2022-10-12" version="1">MGISCSNCQNILRGIDHQQQEHQNDNNQKLDTEQVFDCEVWNFYNLRWFKKIVQIVTITNVNERKYFFDGQLLRIESSKNQSVGFQNFTNLEQLNHLNWSGKYGQLNQKVGKWITTWKGEVLRDAGGWYSDDEKKQGLWIELTKNYRNQAEVYESGYYLDDQRYGMWKYIYDEELIDAGRYNFQGQKNGKWTQLSDRFCYYSKIKYNGEYKNGKKVGLWNIWYQDEYRYINKLMYFIKIKTIQVVVGHIMEMEIKLENGLSRVMDFKDSHKSLILETIETIRKLVDGIFGLSREMVRYMIQQHSQIIYIGEYRKGKKIGRWDIFELGEMIGGGSYDELDSIKIGKWIELSDFFGIRWQTTYNGEYKKGKKINRWDIEKRDEKGKPFYNIGGGSYDSEQGSIKIGKWFEFNDDYHLNQYMTTNGEYKNGYKIGRWETLWTTAFDIINKLIACGSYDEISSNKIGKWIEISDNFCSNSQVTYIGEYKNGIKVGRWDTYFLKHFGDRKNELIGGGSYDEEHGQAEVYESGYYLDDQRYGMWKYIYDEELIDAGRYNFQGQKNGKWTQLSDRFCYYSKIKYNGEYKNGKKVGLWNIWYQDEYRYINKLIGGGSYNGDGNKIGKWIEQSDGFQGFSQITYIGDYRNDKKIGRWDIWFKQGDGQIHDIIGGGSYDEDGNKIGKWVQLSEGFQEHSQIIYIGEYRKGKKIGRWDIFELGEMIGGGSYDELDSIKIGKWIELSDFFGIRWQTTYNGEYKKGKKINRWDIEKRDEKGKPFYNIGGGSYDSEQGSIKIGKWFEFNDDYHLNQYMTTNGEYKNGYKIGRWETLWTTAFDIINKLIACGSYDEISSNKIGKWIEISDNFCSNSQVTYIGEYKNGIKVGRWDTYFLKHFGDRKNELIGGGSYDEEHGLMKIGKWIDLGHLFNSDSQVTYIGEYRSGKRVGKWEIWFEQIYGDNKLQQVGGGSYDEEQGCIKVGRWIELSDNFDEYSQITFKGEYKNSRKIGRWDIWYKKYYGEKTIGLVGGGQYNEQQGLIKFGCWIDVCDSFAASQQCTLTGQYKNSRKVGIWQINCKIYDEGYDINRWVIFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QR02</accession>
    <name>A0A8S1QR02_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2280002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000237">
        <property type="protein sequence ID" value="CAD8118189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="232" mass="28054" checksum="B5B793F2A7299E42" modified="2022-10-12" version="1">MLNLNIFTQDFKQYQKSVKQIIKEAVDNNSTKXSGSFDKTIKFWVKQIEWISQQTITDHEKDVYALSLNEKQNRLISCSDDDYIFIIEFYEQDKLWIVKQRIKQINFGIRLCFINDNLFSCQLYQKDQLYIYELNNSNGQYIKTKEFQVRYSQKDGYCLFPQQYIKQKCLLINKNGQYINLMKKNQNDEFEMGQIIEYQTSRIYGSMSDDGEYLITWDEYSKEIQIRKYQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQX3</accession>
    <name>A0A8S1JQX3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQX3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="93" mass="10955" checksum="B6B5F40A2AF9ECE2" modified="2022-10-12" version="1">MQRSIGYIKNRTKELQLKKQKYKSPDINSSRRIYKLPPQWVDKMEETRDTIQKVGILNQEIEKLKKSKDVGPKAQFNEQVIQGKLDQALQVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSF7</accession>
    <name>A0A8S1JSF7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0080544</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="AMIIECN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035852914" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="2833"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2373"/>
            <end position="2391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2433"/>
            <end position="2453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2485"/>
            <end position="2505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2517"/>
            <end position="2537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2568"/>
            <end position="2594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2646"/>
            <end position="2664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2707"/>
            <end position="2733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2833" mass="331360" checksum="65356E22853860A6" modified="2022-10-12" version="1">MITFLCFLLILKIIFAIEIQEMDQYYQIYNQLIDINDINFDLEDYYFYGVWSKYIPLSPISQVGNIGLFDSNCFNIHNIIDQTSQSVNLIYYDCLDFNQKLITKTIIFSDNERNQHKFVIQIDPFEYENVWYFYYIFGQPQKNKLELIVTQLQKKLLHEIILIKFPFKIIVQQFTFGGDLTVKDSIISSIEKGKQLCYFPGKIILEKFMIGNLIAPQNLTQQAIENFYSYTICNCKLNINYLISDTDIKWLDKNTYSSENMICNSFALQGWIKIIEIFQSSEEFTYYLIKLSANFDNSLSNQNLSPLQFGYKISPTQNFIVLTTYKYSFPQVTIDFQQNPFLIEEMIEIQNDINLWHYLYVKLLEDQLFINIIFYQESNVFEFKKSIEVRQFHYPQLKLQYGNYLQQHKNYLNVQIRNLAFLNCDQYLQSLTCHFSCYDCDGPTKRDCLSCSQESNRIYYPEFKMCLCPLNTTDESKCQSYEDSKLNLISNSITIKCQYGYFEFEDECLKCPSITYQNFVSCVECLQNTLTWSEQPYCSYEIYLNEDGTTQDEFLLLQQYFLFDGSEIYVCQICEQTNLYSIEEQYLDFYLQSLVFKQFCKSYDYLKCYECSITFCLICQVTLEGLRCAQCIFNYKLVDGYCISQYTFPKTQFCISPNYLTSNKICKLCPISNCKYCFEYSMDDISKSTLYRNFEKFDQDEHVRIGCSLCNEGYTFNFNLEKCEFIKPQIQNCMRSFINFNNQEICTLSSLDFNIAPEIINCQKYKPNCLQCVLSPESIIKCVQCKIGYSSSVISGDCYEHLLEQALITIDGDWTIGDGWVQRIQSFMMQFLPNKYFYPQSQFNTQIAAMIIECNQGYQISDQSLTCQKYCSSECLQCEEFQYSFKCTKCPLNYYQFPIRDQINGQCSECPQLCNICKSRSLEDIYKLQPNYILTKDNLIYTKNCIQPINDINVILDPYYNIAKYCFNQDCTYKTQFDVLFSHCEISRGFWPFGYEYDINTNYCNQMGIDTITINFNFQIDDDYCGLLQWLTADTELKQKIFSLKKTIFKLSSPNKLTISTNFPISIKNFDCVEILNIGLIFGDNQYFQINNNRSLVEIKLINFSLSNSSINNINSLLETQTFGNLTLINVTILNTIFVNSSFINLKSQILKLGIEMISLNITNCTFIDTNLFQFSNNFILLIDNLYVKNCKFINSSIFRFLANILDFNKFLLNNSKLLQNSFAQSNFINSTNYMEIKFFNLIFKENCLSKSIVIGFNSNFTINLMDVISNNFEDSTFLSTLLILTQERIFIQFSNLQFSLNIMKEAELVYIFANQQVNNLILSIFNLYLNGSITVPRFDRNHYLFNIQCFQFTLLNVMISNFSDPFLFYIADNYQIDIKQFNFINSQILNKIPLSNACKNQYEQNNNLFLIEGFFLISLENILIKYQSSIDKSIIGIRSSKSYLRDLEGQIRIKDLEFNGNILLQQNQSQFFSLLAIESQGVTKIIFENLRFLSNCLHSYANNQFQSFASLLYISCKTGFVEIINMNSISNSATNTSNSFIYMLSNTIIFSNITISNNNILPQSFWSEYYNIEFDEQYSQEQINQIVQQVLNVENIGGVAVIRAQNFICNFCFFNNILVFKSSIFEITTLGDGIIELNFIVINSTLNNRKLNTNGSGCISINSQNSKLNLKIQNAIFFDVINRMAPSILSVIPSNIQNNILLFNVSIENCISLYNQILKATFSSQIMQNNTITFHNIKLVQKEMAWKQYFQKLGILTQSEINQIIDDNNAVLNIAGSSVLIQNIIVEGLYFAPLFKIQDAQKFFILNCQIQDIQIIYPFDLIHYYEIYHIKSQISFQYLSILNISIYTQNQIYVYSEQIDKYQIQECNHLLRQPQKDHTTFDFETITQILQEKIQLGKSLLYFSSSSNQSQFFLSSVQVNLNNCSYCSNGLIFFQQESFHTLQIQNFECNFNNILSNGCLKFILKNKSLNYVKIKNSNFLYNNGTQGIAVQGINTSISMKYCYIMHNKASLQGGGLYLKQYNEKIFISQSLLLYNIAKEGGGIYLYGDNNLYIQNLNNSYLQFNKAESYGNNLVESPTHLALNINDMEMMSKFSVNQSQIRFLKLDPYLILDQEIKTSTQYLMIPSNQQLKQYKIYLPKEQETIPYINSIYLTLKNSRDELQLNFNKLTCSISQTTVLNTSNKIVDTPSNITLEYDSNNHYFDLKELIFRFDPYNLMNNYLQIKFNCSSDIQHKQLIYLIKARSFKCQLGEIYQNNGCQLCQSQQGFYSVQYDQQKCSIFDKQKFADITSNNINLLEGYWRPHYLSDYSNLCFKNVKFCLGGWGVGNQLCSMGHIGALCEECDTYNIKGQGQYFKNLQNSECLACFGVDDSIIPFIITSLWACFSIILTLKSIATSNRLFVTLKLREKQSKIIYKLSQDHQSILIKMLLNYLWIYSVIFTFNINFSFSFNVIDNASNTSYFMANNLDCYLSEYLQIQLVYSKVITIKILILYQFIIILLGSYVYSKIKKQKHHSNTISHISLCLYIFNYAGLIKMLSSLVSLRVVSNNKYIQGDVSLRYDTDSHYRWITYFFIPDLLFFGYLIPLCLFVLLYAQRNKLDSIRLRGHICYLFNEYNQNCYFWELIKLSKKAIMIFIMTYFETNISLKAILLGLCLVFYQLIAIKNRPYIISKFNSLDLYSGQLCSISIFLAAAKYHNELSNDYVSSIALQVLLIIFSFRLCQPYLVDIIRIYYKKYKVLFFMKLNQILRLIKLDFIVSFLDNKLIQMQYQELQLQRNFNRLRSIAKSQVANRRQERSSRLRLMTSQFNSQWTYRFRQNSPDMEHKSLVPLVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWV9</accession>
    <name>A0A8S1JWV9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histidine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0110325</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWV9"/>
    <dbReference type="OMA" id="FNIVIDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="481"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8047232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="760" mass="89175" checksum="1E304BC313470991" modified="2022-10-12" version="1">MENKTMRYFGMSLNVLISVFLAIVECIINQVSDSIIIDIVLIICGAFFSWIFITQQLGWKKGDISPFFYWTLAIKRITLVGIHRSEFIYFLFGFLNGIYSSKLDVKDQKKYYQKTKTAFQVILILVLIIFNSLTNTIQNQIVLIVFNIGLLVLLGIYDNIEIYPNNTKQNDYNTDQRNTQAELKKCQTIIQQFQQTKSIWEQYIKQTDDWICKVDITKFLFNRSWESREQSQALQKFLKENKMNIQQFFNHLIIVNQQSSNTSQSIITQFELDESNTLLAWIEKNYLQDNQNQKQNVQKLKQYQDEQDQNQQNQEDQPSIISPQNDGKPMFYKDRSQDLSGLLAIPINMDQLGPSNKIYLQCQLNLNSIIYNLSLFIFLVDDDKSNEQKQQSIIIQIKNINKLIKNEMLEQKSSIFYKYIGRMANLSSQMLKQVDLIKQSLQLHLKEFDKIRQSNYNSLSFCDDNAFVKSDKQIGDFKQVLLNVRTQTPIQSAYVKVTSSNSHNHENQDENLQKLSIIYLQQSQELLKQVEQLTFDLLIIEQNNFNFFELFSNPQLKIEQFNLLKTFNIVIDIFKKHQILNQNNIIITQELDDETQMIIQSDRRRMKQILINIINNSIENFDLIQKKKQKEKNENLQIHKSLIIQEQELKPQNSIIIKLQSDADKITIEIQDNGGGIDQERLKNRVNECKFGLAACQKQLKYLAYDSKKPLEIINNLKSSNEVEGSIVKFQLPKTRDNFYLNDDSKQSESLRISQRIGQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0K6</accession>
    <name>A0A8S1K0K6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0K6"/>
    <dbReference type="OMA" id="INGQHKR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="276" mass="32212" checksum="6DC4E2937CCD0039" modified="2022-10-12" version="1">MQNDINLSLNEAPNKQTIMVIGLQQAGKTTFAQKFSAINQKLFQESKIPDYDIELIDTPSYDSFFTLLPFRDPKVTGYIIIFCQLNSEIMKSTKQIRKKLFSINGQHKRTVILFNEFTYFDYRSSEIQEEIMNIKYWCKQQDILFSQLDIKRASCKQISKIDLQIVNLFEKMFMKKIRTTLAQKIFIIIQFGMGIYIFLFAIWLSINDPSLIISNISYEIYCLALLCLGIANIFIPYFFRTGIKDSNTQCINRVNNIQLLSPKFSVVQQLLFQSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1P8</accession>
    <name>A0A8S1K1P8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1P8"/>
    <dbReference type="OMA" id="NDFEMMF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="281" mass="32648" checksum="3388D262BB643FD1" modified="2022-10-12" version="1">MQKNVILNYLQNGSSYYPFGDQPQQNQTNLDPFIPFQRERDKTGKLIKQTDSNFIKINKSNKQCYTDHSYSNSDILNSNKLNTEINQSKQIPSIQPILLLPIIHQPLNQTQIQAIQNSKYTLNQNSIEIGLQLKKLHKKSSPQKISLLSRKTKSMALQIPALGIYDPSPSPKHLPNIKIKSKIQSKIDGLSPKILIQIPPAEKIIKNDFEMMFAKQMASIKREVDQEMKRNPNQIFRVEKFDIKENLNLEEAEQLRNRMKRYFQSVKESFIKIKNLMNVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4R3</accession>
    <name>A0A8S1K4R3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">40S ribosomal protein S9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0050333</name>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0100228</name>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0140189</name>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0210188</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4R3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000023">
        <property type="entry name" value="40S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="12">
        <location>
            <begin position="6"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding S4" evidence="11">
        <location>
            <begin position="107"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8043679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8046190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8049837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8053906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="186" mass="22060" checksum="1EDEC535352E2FA9" modified="2022-10-12" version="1">MGKNYRNRSKTSDTPRKPFEKERLDNELQIIGKYGLKNKREVWRVQLTLARIRKAARELLTLDPKDPRRLFEGEALIRRMVRFGLLSEQERKLDYVLGLTTQKMMERRLQTFVFKSNQASSIHHARTLIRQRHFRVGKRLVNSPSFLVRVESEKLVDFAVTSPFGQGREGRRKRKNAKRVKPNKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K5J9</accession>
    <name>A0A8S1K5J9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHHC U11-48K-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0170113</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K5J9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="4">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8050549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="136" mass="16230" checksum="96DAD98BBCEB7F00" modified="2022-10-12" version="1">MQQYLHISCPYNPQHIILPDRLIFHLSNECKDSIAYKNEQNNIQQDFLKIFRGQLSFQLNKEELEINNNLLTQVNSSNTDIQFEKSISKINEENQIQTQKQMDKQLIDQFKNLNLSYQNQKLIKQQNNLNQDEDMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KFL0</accession>
    <name>A0A8S1KFL0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0200099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KFL0"/>
    <dbReference type="OMA" id="HYEYRRQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019">
        <property type="entry name" value="KDA ANTIGEN P200, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="139"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="267"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="383"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="473"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8052983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="504" mass="63101" checksum="77E238AC83EFD07C" modified="2022-10-12" version="1">MISLINFNPADQRCRINSPRSIMVLKQRGMAQEELLFINKEKLGLMLKQEKSLQDQNLDDFYKHYEYRRQQKVYMLIKDREALIEKEKRKKIESNITQLKGDRPLSSSNASKFMDQVQKRKYMEMMRQRHLQELMLMREQQNQQQQEKMQQFEEQRKKKQEQRQQEFSAKQENIARQKRLKELEDQKKNRQLQQEYFQKEQQRLQQQQIEEQQRQAEAINREQQQKELKQRLERDRKMKEKQAELEYQQRKQEEEEQELRRQEFFQQQQEQKRISAQKRAQSAKIRQREAQRSQIEFQKKLREDYFKKEERIVEQQHKFQEQQRQRQLSIEEKNKMKNEKIKNTARMKEQIHEEFKRQYAEREQEIQYRLQQRSESGAKDEFVKQQAERRAKHREKVRRMNMQQLEEKRQQYLIKLQDQDEKIQRVKKEQEILYQQQAYEKLKKRLDQQDAIKHQRQFEEFRREQIRDKLREVDFRMQQKREDQRELDRLREQANKAELMGKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KG47</accession>
    <name>A0A8S1KG47_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KG47"/>
    <dbReference type="OMA" id="NSYFMSP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="233" mass="27808" checksum="BE7586FC85FBB317" modified="2022-10-12" version="1">MAQESIRLIAKQLNKNKFLIAKPTTDVFQCVQAFILLVKGVKTKDWLLAQLLMSCSLNQMVETIIERKIEYTNLIQCKKLFHLIPQQQNSLGKLLYQLVSQSINYLEEDRSKFQRSKSTQLRNEQSQYSNCFNKIVNYNKKGSLIKCKEDLNKIIQNRKILHDQKIKEDDQKKFELLKEQMKKLNKNSYFMSPRKIQYDQQEQHILSNNNSERKLTNNKQMRSLRSFRQSFYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKT9</accession>
    <name>A0A8S1KKT9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200357</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKT9"/>
    <dbReference type="OMA" id="TIMKNEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="71" mass="8103" checksum="BDA9B76664622E92" modified="2022-10-12" version="1">MQKILKQITNRFKVLITLRFVDLGPDQFNLQCVATNQVLLNETPVHTIMKNEMNQIHVQIVPVASSVCNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KR17</accession>
    <name>A0A8S1KR17_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein OS9-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0230298</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KR17"/>
    <dbReference type="OMA" id="MESRFQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045149">
        <property type="entry name" value="OS-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012913">
        <property type="entry name" value="OS9-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15414:SF0">
        <property type="entry name" value="ENDOPLASMIC RETICULUM LECTIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15414">
        <property type="entry name" value="OS-9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07915">
        <property type="entry name" value="PRKCSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035931997" description="Protein OS9-like domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="Protein OS9-like" evidence="2">
        <location>
            <begin position="98"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8055672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="694" mass="81625" checksum="9B7750E0F75E82D6" modified="2022-10-12" version="1">MLIFLIFTIAQAFNADNTYAIHTIERDVQDNYRDYPELYAIQNILDSISKEITLKDGRKVLCQIPIPQISKSIIVDEQSIIRNLTEDDANNILFPLIGQCVHHTTKEFLYEYCFRKHVRQYDDLNTMLNMQKILKEDFSLGYSNLFISQNYSYTQFEPHNSYYYLDTNYHQIRQQIDRLIIIEGCMAKLRNLQISEKFEYQLNVSTSEKVYTFRVIQVIYNELLLLSQCSDIVIISDYFEIIQIKKSKSVIKQNQYYQYKNIIFGLNLQAQNMFHSQQSIGLILTDKIHIVQVNKIIDFCAIQVSGFLPNIKDQKYPLQIKNLIIMDTNSIYEQLKGSVLIIENILFFHTDNKININDSITIFDNTTSSFSYVETFRINQYYNGIAFLDKQLTKGLNYEGIGFIRRNSQFNQILDIIQPQVDTFEFDEDDLISLTKLEYDLVQVHVFNDSTQGYKIKQQYQKYMGITFSIYQLNNEITCSLFLNIISEMYLTTFISILTTPQQNIFIKTLNGNYTQMESRFQNSSLINQLYWIIVDTQIDILYIGKGNEIRLANKLIQFQMQRKRNISSSIIFEFDKKSISLKIYDIMILPLINFGLFTTTFSSEYYHPLNYTGEINGIFEESYRFGSFCNPINSTRYSQIKIQCHPGDLMRFISVTEEEICKYQIKIGTYLLCDKKQDIIEPSEHPILCILKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KT09</accession>
    <name>A0A8S1KT09_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0250233</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KT09"/>
    <dbReference type="OMA" id="YFYSKVY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="185"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8057055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="244" mass="29178" checksum="082E194BD5F2A9DC" modified="2022-10-12" version="1">MITNNYLTSKLLKKSNIENRQVNDNLSKNDKENYYKQWEESLKRKERLLESKHQELKIYSKNLQIQSQGLLKEKLDLGEKIEKYNRLLEQIIDQQTSDKQLKKSSSLIPQAYIKSNKNGINECKERVGNLLKNIRLTSKSHLKTQQGSYFSNRTEFEEILFSKRNNHSCSFTGIINLRNKEELISKQQQEQQRNSKENKERFSHQNTTQDELINHQKSLQQLEDNQKYFYSKVYEELNKLKKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTT1</accession>
    <name>A0A8S1KTT1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0250027</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTT1"/>
    <dbReference type="OMA" id="MWAGPYG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF2">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="3">
        <location>
            <begin position="16"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8056732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="350" mass="40149" checksum="E4EE361A4EC2692C" modified="2022-10-12" version="1">MEYRRLGSTGLKVSAIGFGNWLNADDNETLERNTKIIQKAWELGINFFDTAEGYGKGKAEIQLGSALKTLNVQRQDIVISTKIYFGTAQDDFPNSRFLSRKHIIEGLSNSLKRLDLTYVDIVFAHRYDPQTPLEEVCRAFDWVVRHGWAHYWGTSEWTAQQILEAIGICDRLKLIKPVAEQPQYNMLVRDKFEWGYNNVFAQGYGSTIWSPLYQGILTGKYNEDILADGRFKNTDNPYLKSFYERTLGNPEYAAKVQSQLKQLGDLAKELNITQAQLSLAWALKNKDVSTAITSASRVEQLEETFKSIQAIKLITPEIETRIETILQNKPETQLNFKVQKPFPTRRDLYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWH0</accession>
    <name>A0A8S1KWH0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0290065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWH0"/>
    <dbReference type="OMA" id="ITIADTW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="2">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="305" mass="34889" checksum="7B0385B2777C464A" modified="2022-10-12" version="1">MIASNELVLGDQPKQIKCPFCSNSTVSTVTFKNGYISYITCFLVLLIFGIMASLLLIPVIMIITRTLIHKCSACNEDIGGDGKVFHVLGVKDSMMSFKIGEFGVILTKRIVIGILLLILTLGVLWYRIIDLPTSQWMDYHQHHNPNITITDTWTDYLEKCAKQKIFQNLQFQQICYQKYIDRTIVDWKGYAIRVEDNQQSLLKFLHHSVNILVKMDPAESEFHPDLLLTADSEIASELSSEIEKLERGSEFIFTAIIKSIGDFQNIRHFHLLNITKLDSYIEIPPHLHQQSRYGNSQQFLASSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXF1</accession>
    <name>A0A8S1KXF1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0290234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXF1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="106"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="269" mass="31752" checksum="4466C01218BDF14C" modified="2022-10-12" version="1">MRQQISTPTYGVGYKTPFSLSQQGAVKLVSQPSFQNQVQPLAQQQYQQQQQSYPQQFNRQIYPINGNTNMNRGTWEQQGAVKRFQTSQGNGSVQSQLQVVDLQQVEEPWKIKVQELQNRIQELESQQQSKQDNEDEESELSQAYSQIQQLVNTIKILQDEIQQIQEKLEQKQNIIDSYDRQIQDKDKEIEEANQYIQELHGQLEEQTTNVEEKHKYLFDEVNTWKKKFIEQNKEFHQKQEELMILQAELDNLKNAQIIIGKSSDYKQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXI2</accession>
    <name>A0A8S1KXI2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KCEQQIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1043"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1233"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1351"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1602"/>
            <end position="1623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1658" mass="196400" checksum="0515EC651B067EBA" modified="2022-10-12" version="1">MLQGFCYVSMYQPNFTLNLKIQKIVLFIYYCQILTLAFPIDGWNLWNYQDGALRMMKNLLNVILIFPFVIEVKSSSLIYILIVLFSLFNLFMLSAIFWIVKFKNKLSFIVGITYWYLVLVPKLFFIPQLFILIGSLSFSQRGLLYSNFQFHAFLPISILSILVLTYNCFFSIYFIRKMRLLKDNGLIQKFSQLCFLREMLVIAIIFIHFQNRIPLINALELILMNIFFFTLLLEALHFDIYHPEIQKFALILISGCIGLVLIISINVISKNKLISEEQLIVIQLIVATLVVFICIFSQNRKIVYQLQGYSESHYQIQFVEWLFYTLIELNQKRKKQQNLFFYIIFIQYHQKTCINCLKKVLQHQKITAKSIKFNVLNCVLQNALKLQHINFGSDYELLEIYYVDFLNKIKKQPLPAYVTLKGFVLKHQMISYHLKATIKYLFQELEDIILTDNNLVESNNHDYEKNYLDVKNFIQAYSIEEQILPQIIQLIDQKIQIWNEQINGLDTIQDLEKLIVNYSKKLLICQNSLQSQLQIDLMNLNQIQKVRNVIELRITSIFLLMIFNDFYGSLKCEQQISDILIMENSLPNDLISNIDILHDNICLIMVSMVKDRGFIKNNHKNQIANYFGLDIKELENNNHINLFIPQYLLEIHTQLLQSYLETAQSSLFNQYQIVFSKLKNDFVQPQQLKLENNFCFFDDYILTACLSKVKETSEFILFDENGRILSVTQGFYTDIIKQSFQEELSAQTINQAYIFLFFTNIFSILESQIDNINQSEFLQVELMTTITIYENLSKIIEFLYENKITTSSTIAYSLKKNVSKTPIQSKPKLNTTQKLKKQNYQTGRYYINTSVKLSDEIQLLCPKFINSVLEFMQQYNQFKFVEYHTKIQLQYRTIGKKQLQRSYFIIEILDFRKKSGQNNAIKQLHSINLKNIEQIEKQQIQKQFLFSDQVESTQQKEFQKYNNDIQQNHVSHLNEISKSGSEKHLKQRNNFLKLLSYENDLVCLQKQENQSLSSQSSNTLKIQNLIIFNDFQFQTKMNKNLKIILFFTMISIMILVSIITYNILLIRYQLSEQINSSSSLNAPLLFNRYFFQSYALSWTLLMNGLNIVDQSDYLQNQTSFTLKNMERETFQNLKIENMGLLQNISLKILQLERQTVSYTEFITYIQNTLQYLFQFNLFSKEHRQKVLVLDFVNSVVTLRYNIKYVFDMNKELIESLDQMYQNQIKNNNSNLKTVLITEICIIFIFQFIQLYFWKNFENQKQQILILVGKFSESKANEMIILHQKYKSIILQNEKQQINWKIQNFTQIQPVNIVGEEKKVFKMNIQKRDKIKGKGILNSRVINNSYSNKKYIFYYIILFLILFSFLFGGYFYYSFLMKKLQPQQQLAINFIRFSSYFDTLITTALVIKTQPQIYPAIVSNKIYTQTQMNKYRDPLKQLFHMFLEVYEVYDENLTQIYEGILFSQDIDDSKKEILLGLYEKDICQIIIEEIPFCQYDQLGEKNFIQKYSQFYLQDNNRDYLKNGLAGITSSVSYFMKTNYDYEISTINYITDFNLLNKLYLTQEFNNILIEHFSSTTQSTEKVLNLILSTNEELMNQNKKTITIFFSLIGSIILIIYIFFFTWILKLSSLKLKYLKLGLSLVPKEIVADQYTISIIKLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L446</accession>
    <name>A0A8S1L446_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0290024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L446"/>
    <dbReference type="OMA" id="GGYMYIL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="251" mass="29947" checksum="430B7D03012A29EC" modified="2022-10-12" version="1">MQKTISTSDQDYNIVKAENHQDSDEELQEKQEKSSKLVIDDEEELKEVPLEQNEQNNTNIKQEVQQHQQVQKQQAIQQQPNDQPKKVVAQVYPKFIDFDYQDINIKFKPEWKDKRKNPEPNKFEKKTVIEINEPVLYTMNKQKPKHGRSFFTSLFSVDYRYIFIYGGYMYILQLKNDKMFVKSVNSLQRLFKIILKGDQIQLCFINANLDKSKPPNIKSYHFLKGKEQKEKLLMRIRGEINKLGYDFDLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5F6</accession>
    <name>A0A8S1L5F6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5F6"/>
    <dbReference type="OMA" id="MYHRRTQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="395" mass="46515" checksum="7A512973566E26CE" modified="2022-10-12" version="1">MRKNTRFSTDSDRKRIISNLSIKSINSTTINTTTDATLPRLESNYFIKSRLKKSTHQIRFNRSPMKMSQPNNMLENQSIKQSTKILLNSVVFELDKLAIALKHNSTQAQDILNLTIDLMNMLGSDYDRLEIYLIVLQNKCKSQCVSLGLLFTFLIAIIQETYGHTKGALTQYKYMNDTANKHQLFQCKMMVYYRIGLLLNRLKQFEKLLVIGKKLLKLSWYYSDQEYESKSYYLISKGYNGIQDMEMSVIFMNRYLDGILQQNDHIKNIGIQQIAMYHRRTQESRHKSPSSDDFNLEIFRQKMERNYIKMKMIGGKLAFKLQSSECDICAQYGRNYERIQTEDEHPYKKNLVLHTDSRNRDLIGYFDSEQIQQMYIPSKTLPILKQLILKFISYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6F9</accession>
    <name>A0A8S1L6F9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0340128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6F9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003923">
        <property type="entry name" value="TAF10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03540">
        <property type="entry name" value="TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="136" mass="15977" checksum="64DED9BFE6EFD290" modified="2022-10-12" version="1">MNQQQSDIPPSKKTQFTGSIDNLIYQTLSKKPNMSEFLIQEILEASGFQTNDQNCYKLIAHLTDKWMIEMTDSMVQKLIQDRIRKKEKDPDEKLQLTTNDIVNELDQRGLKLHNPYQYPEIDAHNLQKVKNDIQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9U0</accession>
    <name>A0A8S1L9U0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0290125</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9U0"/>
    <dbReference type="OMA" id="EIYEYFT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001060">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF411">
        <property type="entry name" value="OS02G0248440 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="494"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8059704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="549" mass="65536" checksum="60DDC8D8362F8955" modified="2022-10-12" version="1">MQGQKRTNIVIHEIGPEKCVNCNQLISPIQLLNHKITCLGIINIEQKNSDQKQEQFIQQIVIDQKPLEQLNQANQYQFNPIQQQIQIQKIASPTQLKQPFQFKVEQKESNIKQGHLEQKVNLQIYQNDQQNKHSLIQQHYIEPNEQGQQIINQEAFINTIQTQAQQDLNQQQNQYPNLFEDNQQQMHNLDPYFNIHQQKHLKIQQLNQVEFHQIFALKQAEQSIIEKQEQIKNNQNYKISQNAIVNKNNSEQKKEENYQFNDANQVKVQQNQNQDQIKPFTSKEQLFLNHQQFCQNLYQPPLNSPKNQSYLQQGKKPKIVEQNQRDYEQYKQQSKQQQQLQQQQQQKQSAISKEQQRIINKTLFEESKNNQDNIANIRNPYKQKKQSNQAQIIVYQQQQNQYQQNRRQVESKQQYIRQKDRNTERYRAISDFSDRKQFEFKPGMAITDQQVNQMTPEEIYEYFTQLDLENQVGLKSKIFVLEDKRVQVNVTDNCAICLEEIQPQKEPIDIRLDCNHQFHYDCIKKWLLKSKQCPVCKDYVISATQKNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LC70</accession>
    <name>A0A8S1LC70_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0340131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LC70"/>
    <dbReference type="OMA" id="TTEDHEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="85"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="30643" checksum="DC075D353395F7BC" modified="2022-10-12" version="1">MFQAEEKTLSEQQLILHLIHQNDYDTLKELGINKANINFLESFNEIEVDQKISPLICACYLGRLEIVKLLLSNTQVDVDLASMDSGQTPLSIAAMTGNYEILKILLDAGAEVNKPNTFNQTAFIMCFARLEEEKNVFENRKICFKMAELLLHYGADINWIVDKTHGFNLLMQLCSIRMELNQKEADINYQIIKFLIENGAQKDLQSLKGKKAQDLLKKHSNRDKLMELLHNTQQLYFYGKQKNSSRYSNQQQQLLIRNVPRQQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LE72</accession>
    <name>A0A8S1LE72_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LE72"/>
    <dbReference type="OMA" id="LSKLMNW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="568" mass="67219" checksum="04D682094947F7B2" modified="2022-10-12" version="1">MNIFSERSSSIYTQRKFISECQTPSKDSKGYISLSKELNMPITQISKKTASNQRKKIKLFDSPYLNDDTYTTLSNNQQKNEVDILSKLMNWEKMHLNQYQYTSKHYNDLKPKTEYLRNQNPKFIDINQKEQEIYRDQFKTQKQAPKSQFIPKQAQLEVQLDDMESIQMKNLGPQYAQTNQQQLNQYIISIAKTNNRILPKIQLPDKNQKNNLLPNLNRQNRIYSVLNDLQLQQMLDKDDSIDNITELQKSLFRKRKTQQKKSMLRKRIIVVLAVIRISKKYRVILKERAQKTQNLDQQKSVHQKYLDQFRTKNLQYHQKDFADGIFKKLNSILLDKKYIKECEQISRLQDFIQKDIKKQRFCQFISLFLQSLEIATNQNVLPLVIVHQLNLNFFKTNNTQSCLFVLNRACYYSTLIQKITKKQQLLIATEYLLFQLVIPNIFQILKDMKIKSVEHNNQTLSYLTALTEMVQMLFVDYFKNLEQVSSKNAKPIQRILLLDIDDNSGIANSEVIITSKINQDETIIIENGFDKSSIKELQSCKPYWDEKMKSRFIKIISNIEKHILIKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LEG5</accession>
    <name>A0A8S1LEG5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein/NADH dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0350223</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LEG5"/>
    <dbReference type="OMA" id="RECTGIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016464">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B8 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005822">
        <property type="entry name" value="NDUA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8063953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="100" mass="11657" checksum="10E9F295BABD0093" modified="2022-10-12" version="1">MSWQKQLGNSVKELRFVFCQTCGRSEGVRNLVTKNYWQWKEANPTFPFVVRECESIDPYILIRYKYGIEKKALIGNLNEQELEQVLGQLIAQSNKVNSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LG05</accession>
    <name>A0A8S1LG05_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0390220</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LG05"/>
    <dbReference type="OMA" id="KIKYLQP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08215">
        <property type="entry name" value="STKc_Nek"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000172">
        <property type="entry name" value="serine/threonine-protein kinase Nek1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="9"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="384"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="545"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="583"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="708"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="617"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="384"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="396"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="411"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="545"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="596"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8066637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="801" mass="94190" checksum="88AF77B0483C72AD" modified="2022-10-12" version="1">MSQNENEIYRRIKLLGEGSFGKAYLVECIQDGTLCVIKQVDLNQMKEDERRETIKEARILEALRHPNIVKFREVYKTKKGRLCIVMDYADGGDLSNKIKQTGSCLFSEVQILDWFTQICLAIKHVHDRKIIHRDLKTQNIFLTQDGIIKLGDFGIARVLNHTREKCKTIVGTPYYLSPEIIESRDYSFKTDIWSLGIILYELCALKPPFNAESLHGLALKIVRGQYNPIPDKFSTNMRQLISSLLQVDPNRRPNIHEVLKMPIIVNRIRSVLSESIRNVEFSHTILHKQNFVNHNLIVPYDTELRSVCSQPNLGKIQQQQPLQQYQQQQQPSNYLLAFNARGQPQNNLLKQKQLYQQHLPQNYDYISKPSQQNYNPYINYQKYQQNKQQQQQIQPLQVNNRPNISPLNPQRNEKSPYYEDRSPLSKSPFNKDVRDKSPFEIQREQVRQQIKRNEEKFQQLQENKIKYLQPDKYELPKLSREPYSQPQIQYKYQKQQSDPVSVKDPIRLEPINNNQQQQQQQQQQQQQQQQQQQQQQKIDLQQKIEQQLQQQYPSSQRDQKEQRDSVYQAPQTERVQVAQYISQPPPTQQQSDPIQKTESVKPSKQQLKESQLILQEIREIKNDNKDDREKMKQLMEMKRQRNSNTQSEIQEVIKEASKNSDEQEDEASQLLAELEDIVIAAEGGDVKHSQVINVGDCDLINMQQSVRESLDREDDDSLEQKSNGSNENGERENFQDDEDIVCETPKELLYQLLLRQIGQEQLTKALNKIQNAYSQDYINLMQNGLDELQQYKALIFMYECM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHH0</accession>
    <name>A0A8S1LHH0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0400195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHH0"/>
    <dbReference type="OMA" id="MYQFTKK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="165" mass="19766" checksum="475A3FA1FCD44AB4" modified="2022-10-12" version="1">MNFLNKTLKTASQSEPDLQQYQDMINGLENVCQNQSCNLHDKKKQDRKKKLQYQIFNLSLTKTTLASIQTMYQFTKKTLRQLNQNQKVTIKQNFQESMKEYNYQLYKISKNLPSIFNTQHLQTFRISNWYEKIKSTCFNNEIQKNEQLILHNPGQRDEDNDFIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHZ3</accession>
    <name>A0A8S1LHZ3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHZ3"/>
    <dbReference type="OMA" id="MIEQMRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="88" mass="10523" checksum="7479986B60C6C842" modified="2022-10-12" version="1">MSFIYLPFHNLAYCPKQVIEFVQNPNLNLQDLTHMIEQLRMPKNHLKAIQKMQILASRQEQFLQDENTMQNQEEDIHSPKQLLSQIPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJ81</accession>
    <name>A0A8S1LJ81_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJ81"/>
    <dbReference type="OMA" id="WELWSIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033579">
        <property type="entry name" value="TMEM128"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31134">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31134:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20479">
        <property type="entry name" value="TMEM128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="188" mass="22013" checksum="F932A74956A20F3E" modified="2022-10-12" version="1">MRRYVPVQNLEEESTRQPKIIKQLDIYDKIEQWGEWLWLKFQAILWISAAVAIVYYSNFFKQLFHNENINELFLTFALLFIGMSISLSLYVAFYLPCIKGITEDIETYNPKAIQLGAFAGFMSFITLTIAIWPVFGWWSFPMLFAMFLGFINTGHFLPNHQLSGLLFGAIFIGAFFSHYYIDHDGYMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LNB3</accession>
    <name>A0A8S1LNB3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0440052</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LNB3"/>
    <dbReference type="OMA" id="MIHQNDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24203:SF45">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24203">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="85"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8069490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="30714" checksum="AFE659301D933B2E" modified="2022-10-12" version="1">MFQAEEKTLSEQQLILHMIHQNDYDTLKEFGINKGNINFLESFNEIEVDQKISPLICACYLGRLEIVKLLLSNLQVDVDLASMDSGQTPLSIAAMTGNYEILKILLDAGAEVNKPNTFNQTAFIMCFARLEEEKNVFENRKICFKMAELLLHYGADINWIVDKTHGFNLLMQLCSIRMELNQKEADINYQIIKFLIENGAQKDLQSLKGKKALDLLKKHSNREKLMELITNTQQLYYYGKQKNSSRYQNQQQQLLIRNVPRQQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPB4</accession>
    <name>A0A8S1LPB4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0420270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPB4"/>
    <dbReference type="OMA" id="CHMEAQH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="247"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8068659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="37164" checksum="ADF03B9BAB4DE95D" modified="2022-10-12" version="1">MSALKAIVTLDTGDKPEYIHIYEGDDIEMLAEFFCDRHNIQEDGKQFIIDHIKDQLKLQEKQIKQKFVPQTNSLKQCQSQAIIYKPAQVKKNDYEELHNTVWAQIQKKPSTTQLHQRNTSNFITQGQKSQGNLLLKKNELKNTKCITDIEQNKDCKQQPKRSRQEQIDFANRLISQKNVSEKRLQKLRIESNSPISHDFRPKVTKDNIYKKVSKKIDQEDYELAQMIGIPQRKKIFEFAQQKIKYKQDQDILKRIFNQLDNDKDGQISTNFINLDIDHQILEKIKPVLCHMEAQHLILDFDSFSNLMKSFQILIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LRX9</accession>
    <name>A0A8S1LRX9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0430149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LRX9"/>
    <dbReference type="OMA" id="WEDQKTE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="15208" checksum="8B8437E6C94A4721" modified="2022-10-12" version="1">MLNNQTNQEQIYSNDNQLENTCPQTKLCKQSDPIAEWEDQKTESILKSNHLSITSDDQSQDRKRFYSNNEFRKVSFDESLNTVHTYSKDNKKEIKNFETSFKHKKKVDKSDIPKSKRFGNHKQRKNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LT16</accession>
    <name>A0A8S1LT16_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0430290</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMEM43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LT16"/>
    <dbReference type="OMA" id="LAEEYAC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012430">
        <property type="entry name" value="TMEM43_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13416">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13416:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07787">
        <property type="entry name" value="TMEM43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="375"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8069285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="434" mass="49656" checksum="4EFC9D739BCBEB08" modified="2022-10-12" version="1">MQIYTTNGTQHLRDHIDNKKHLSSSSHGGGSPIAAIFFGGLFIVGAFCMLWYNERRQAITEYRINQAYKECTSVNSAEINPNTNNKLIHTNGQSHTNSLIIDDIFGLKLSDCVKLIRTVEMFQWVRRSRQENNRTTYYYEQIWSSTFHAEVGDGYFNDQSKWIVQAQDQINENVYVGAYLLTKSLAEQTDANEIINLTTEHAQNVAKYFQNQRIFQHFDAQGKEMYFQQEKGRIINNDLRVSFRAARIGPTTVVSQQQNNTFTPFIIHDKYEQTLNQDGENIENSNPFGILSCCCCCCLCCCQLCKAIEQPLSELNLLYQVILTQQQVFKKLAEEYACLTLCQRITGYILMGFGFYLIFYPVTWVVSIIPLIGEFLAAITGFAFFLVSFLISIPFSILTIALAWLFYHPKYGIALIALSGLIGTAIYFYLKSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXN4</accession>
    <name>A0A8S1LXN4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0450154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXN4"/>
    <dbReference type="OMA" id="GSTCRWQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035776627" evidence="1">
        <location>
            <begin position="22"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="84" mass="9497" checksum="C776AEA093390784" modified="2022-10-12" version="1">MKKLIFLGALLLLLQITYVNANCMQLAQDFKCTYMHLQCTDNDNGECSCEDGSTCRWKDGIGDDNCPCGGAKPRRRRRRVHDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M031</accession>
    <name>A0A8S1M031_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0500099</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M031"/>
    <dbReference type="OMA" id="EDIQYTN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044542">
        <property type="entry name" value="NAA30-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45896">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45896:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="12"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8072889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="172" mass="20268" checksum="27F8A6093298B18F" modified="2022-10-12" version="1">MDQDQSIKLEDIQYTNFTDYSQIPKIMPMIDAELSEPYSIYTYRYFLYGWPDLSIFAYYNNEIIGVVIGKLDKHNKSGRNRGYIAMIVVENKYRRLKIGRILAQKFIDKIKEKGGEEIVLETEQTNYAALRLYESLGFAKMKRMQNYYMSGNDAFRLKLFLVDPVDPHLKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5V6</accession>
    <name>A0A8S1M5V6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NF-kappa-B-activating protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0500194</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NKAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5V6"/>
    <dbReference type="OMA" id="ETMKEQR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040466">
        <property type="entry name" value="NKAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009269">
        <property type="entry name" value="NKAP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13087">
        <property type="entry name" value="NF-KAPPA B ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13087:SF0">
        <property type="entry name" value="NFKB ACTIVATING PROTEIN LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06047">
        <property type="entry name" value="Nkap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NF-kappa-B-activating protein C-terminal" evidence="4">
        <location>
            <begin position="197"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8073075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="305" mass="36291" checksum="19E1A665DBABEA5B" modified="2022-10-12" version="1">MKKKWQNRSSSSSSSTSRSREDEVKNQRSKVSYQEYLLQRKQRRESIKKSNIWQFNSDSEEVRKDLETMKEQRRQQRYIIEEQMKQEEDKLRRAKLDAIMKGEHLQKVLPSKSQEREQQSEPKEIVIQEEVVNQQEEIDQEQDEEEQYYSETDSDDSFAEKVRKLNEEMQKKKEENDKSCQIGPMPLLFDKNLQHRTNYGSQLLPGEGAAIAYYIQSGKRIPRRGEVGLTSEDIEKYEGLGYVMSGNKHRKMNAVRQRKEMQILNAEQGKALMIFNYEQKVKREKQIIDQLQKQLKLKQTNQTNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M706</accession>
    <name>A0A8S1M706_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0540266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M706"/>
    <dbReference type="OMA" id="NSKNECY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="32628" checksum="9530B121D986E50D" modified="2022-10-12" version="1">MGICSGVCQQQAKNIVNESAIITETIKQALQEDAITNKEIYQEERYLHMETGAIYEGDLINGIRSGKGKQVWPDGSYYEGDFVNDMADGKGLLVNAEGSVYDGEWLEDKASGFGKYYSHDGLYYEGQWLEDKQHGIGKEINSKNECYEGEFVRGVKQGTGKLTINSGEYYEGEFQNGMMNGQGTYTWKNKKYYTGEWKDGKIEGFGIMKWEDGRYYEGNFQNGKQHGEGTFVWKGKKYVGAWINGYQEGEGTYYNKQGLSRVGIWKNGKRVEWLDDNSSNNSIEQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCG7</accession>
    <name>A0A8S1MCG7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0520182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCG7"/>
    <dbReference type="OMA" id="HAIMMAS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052775">
        <property type="entry name" value="IUN_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190:SF1">
        <property type="entry name" value="SI:CH211-201H21.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190">
        <property type="entry name" value="SI:CH211-201H21.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="340" mass="38985" checksum="1C1AC6C133830181" modified="2022-10-12" version="1">MTNNFKIPDMLLGNDINQYKKYTKFIIDTDAGGDDAHAIMMASYILKYIRKDAELLGITCVSGNASLYNVIKNVYITSRYCHFDEFPKIYKGCEFDSMRKFHRDFFFKEDGFGGVQNQYLKELNVNENQQLYENKHAVDFLKESIEKYQDELCIICIGPLTNIYITLLQHPQIINQLGCLFCMCGTYMGVGNQLNGIAEFNIATDVLATKMVLQADFKNKIILPFDTVLSYSLNKDVGSKIFNVQYNKIAKLIHDIYENVVFETCYYLCDELAIVMAMMPETITQALVRGIHIVEDGFARGGMIVDWIKKMKKEDTSQVAIVTQFNWDQMVGLALKTNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MG18</accession>
    <name>A0A8S1MG18_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0610106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MG18"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="305" mass="34300" checksum="9E0B53F948A80BCD" modified="2022-10-12" version="1">MATPQSLSPAPMQSLGDFTAKKLVEDDFSTGKATLKVKASANGGGIANYKGTLDISKSLAPGQETKFQFPYNNYYFWFATRKEQTKVHVDFGKFNIIKQQNLFANVQFYNSSSKSTIRFGSVYEGKKCLSHARFEWLNQDKVNFLLRSQVKHNNWVYVIASQLCLSRQEFDKLDFLVGYQTKNYDVYLRHLTNQIPSFPLFSNFGFGKFLIDAVYRQNSNQYGVEIEYTPVSRLSFLLAASTKFQGADVKARLNILNKTVGLSAKGKFNNTFSWTLATQVPVDGSSPSKCGILPIPLGITLETTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MHU5</accession>
    <name>A0A8S1MHU5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine/threonine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0530192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MHU5"/>
    <dbReference type="OMA" id="NDELQWR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000161">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000205">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46422">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE BSL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46422:SF7">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE BSL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="2">
        <location>
            <begin position="472"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="824" mass="94135" checksum="67A008158EED06D9" modified="2022-10-12" version="1">MESVNFGGDNPQPRFGHTICVIAPNKIALFGGAVGDTGRYVITGDVYIGDMTSKKWKRIEASGSVPTNRAAHQALAIELNQMIIFGGAVGGGGLADDNLYVFELRDDTGTWVTVPVIGTTPGRRYGHTMVLIKPHLIVFGGNTGQEPVNDVWSFNLEKSPYSWQKLECSSEQPSVRVYHSAALCTTGSANGMMVAFGGRTNDQGALNDTWGLRKHRDGRWDWVRAPYRNQTEQPLQRYQHSTLFLGTLMMVIGGRSNNVGETLPFEIYDTETSDWYKFQAIQRFRHSSWLIDQFLYLHGGFDSDQPNIPTEGILRLNLNTRFAQTPQLLRQLNTLKTDQSFTQSFNPRPPTNQTQTQDQFRRTNQQQALAKNQNNNQQVRVSSATNKNIRLANQAIVAMTYGPEEDITNQVKKVPIDKLQDEHKKLGAGFQDPNSQNKSQFLDQLCQPFVQNLLIPKDYQSIPPNSNLLTGIRKEMIIKLCDEVQRVLDKEPIVLRLRRPIKIYGNLNGQFLDLMRFFDHFKAPYDNLYNGDIDSQDYLFLGDYVDRGTRSLEIVLLLFTLKLKYADQIHLLRGHHEDAKINKIFGFADECFLKFAEDIMDPNSIYQRINRVFQYLPLAAVIEDKILCVHGGIGQTMRTVDEIELIQRPLEIVHDPKTYQQKIALELLWSDPCLSEDELENQPNPERDIFQNRQIMRFGTNRVSKFLQENNLNIIIRSHEPTQEGFERQNNNVITVFSCPDYGANSQNNKGSMLTISKRGDIIPKVILPANSTSESRWMDLEEAIQRKKGFAKYVVIDNDELQWRKRQFTPLRSKKSQSQKQFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MIR2</accession>
    <name>A0A8S1MIR2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0640001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="SPINHIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="488"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="530"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="572"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1185" mass="135661" checksum="558E0FEE447ADE1A" modified="2022-10-12" version="1">MTSVNLNKLVETPYMMEIIVQVLPNMILKATEIINIKQTFLKNFSKMLKRIQMYKSQQKRHLQESINEVNKESEAEIQVDYDEFLKVTHNDLQNLETIDYHQIAIGVWNIWKKIQSLNNQVHKQLENIFETNLLLLNQILKQIKIKQEEIIQFTNDALKEYNLTIYDFYSEFINYYNLKQIEKQRNLGKFIDTDRFLHDLLEYSIRLAKKMSKNEMTQDFDIKTKEKKRKEEQWLNEFFNYDDQYGAYKKDIRSCSLVQQKGANFQFTHKSIYEFLIAADIYEVQVQSKNLDTYILNIINESLSKEENQNQNCIEFLTNLKQNYEQNYRKIGNLSLVQKQKQLDAFEQTIYSITRLITTIKEHDINSINYSTEIYAETRLYLIKKIYQEVRIIEFLKFLVHLTKIDNKFIISGSNALNILVETKVDLTNQNFENIRIKNTNLFGGNFAKCNLSKSQFKNVNINGINSNGAQLIGCNWQQLKINDLDQLDGHNSTVYSICFYPDGNTLASGSGDCSIRLWDVKTGQQKAKLDGHSNTVYSVCFSPDGNTLASGSGDNSIRLWDVKTGQQKAKLDGHSNTVYSVCFSPDGNTLASGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYPSMGNQNKNLGQKYKISFFQHPFAVKEYYLKFRYIQYYGLPRLQYFKVQESFLQKTVSFFGELLEQIIISYNNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJ33</accession>
    <name>A0A8S1MJ33_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0630267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJ33"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="57" mass="6940" checksum="D813D4CC7E1DAC21" modified="2022-10-12" version="1">MVEEKKWKFLLKLQLEKQLLQISNQKIQSAISRNKYQIRNESIMISIANFCRIKFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKZ8</accession>
    <name>A0A8S1MKZ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0580147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKZ8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="71" mass="8944" checksum="7AF9FDC11E14D316" modified="2022-10-12" version="1">MRQIKRSTQLISNVINWLQYQKGSYAIRKRDNYQWINSWESIFAYKKMETNQQLMIFFFKKEERWHKYCGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MM52</accession>
    <name>A0A8S1MM52_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0660050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MM52"/>
    <dbReference type="OMA" id="MESNCKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="420" mass="49695" checksum="2C97F294637D10C4" modified="2022-10-12" version="1">MGGCGSREKEIKEKIGFSQNDYDYAIKRFSKMTQQKPGKTDQYFSFEGFKTYFTENPVFAQKLYIFMRNYGGQNYVDLNTFLTVVDLFTRSSMKLQSQLKNLDIYILFILVSLSNSEIMHKETFNFNNIYKISISYSNGVKLFKELINMQSDKGDILCHDDDQAPILLVNNIFEDHSILNYKIFNDKIKQETPQISKIVKNYIAGKFINKTLKNKLPKMESSELMNKQLIGMIFQQILIKLFLIQVFLGSINVQLLIKYTNMNLKVDNLIISIFLETVSYKQKDLMLYYLDIIKKIKIQIKQRNTLLVIFHLLNGKFHLIYQGIRVLLYFLFIQNLKYSQLMTNNKVNLLYQYPSQLKNRVKLFIVNLNKDQNNQVQELVVVDMIITEFGQMESNCKQVDQWMMIRPFRVDQQYQIIFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQ31</accession>
    <name>A0A8S1MQ31_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SNF7 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0670185</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQ31"/>
    <dbReference type="OMA" id="GVKQMQK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF10">
        <property type="entry name" value="GH13992P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8082300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="25403" checksum="D9747FCE89AEA65F" modified="2022-10-12" version="1">MNRLFGTSKQEPPKQQPPPPQNPQQPKVIDLTEQQKRSEAKVKELTEQIQELDKQISEQYYKAKNSKGVQQTTAKSRAVMLLKRKKMLEQQLGQMLNNQMTLDQVAFTKENIQNTLEMAQAMQQVVAVQKEAFKEIDMDKLDDIMDDMEDMKFETDYMNEMMNRNYGCDIDESELDREMAEIESDMNYNAFQPQQQQQQASYQSLMGQKQVFNPQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MST3</accession>
    <name>A0A8S1MST3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0670226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MST3"/>
    <dbReference type="OMA" id="IVTKYDP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="215" mass="25529" checksum="4C8CB89435020679" modified="2022-10-12" version="1">MQLEQRVSKLEKLTEQLLGRICELEDQQGDLQDQIKKLQTKNSQLEQEIGNLKNRTEEIQESWLFYCDKKRSLNSIKQTLQIESDIVKEFDYQSWVTEDIMWRQIIKNICKEQQKDLEKLNGAQLKQLAVQKLKENIDNEVLFVLRNVNKENEKMNELIELCAIFTQLWYEIELGGEQCQGRMILVIESEINLDKLELTRQDNSKVILQIEKLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWC8</accession>
    <name>A0A8S1MWC8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0720141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWC8"/>
    <dbReference type="OMA" id="ACINQRS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="36"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="219" mass="25813" checksum="0F9D97DED0723EE2" modified="2022-10-12" version="1">MNRHEKQEVEQGLRNLLEEIMLFCNEKILGIENCNCQDLIQRLSKLFRQLRNSNLEYKKLESVLQQQEADIRNHISLEQQMKLYQDGLQTKLEEANQEIKRLQESISKLKKFKYEYKQCSPKKLSSFSQHGGYTSQRSSCDELFKRYNKLLQQQQASISQRSNNIQISQYMMKGRQTLAEICNIQPNRSQTKNNNNSLNKSGYNNSINSEAIRNLLKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXK2</accession>
    <name>A0A8S1MXK2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0670128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXK2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000089">
        <property type="entry name" value="Cyclin, N-terminal domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000283">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="147"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8082186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="369" mass="43448" checksum="974AE52101105D31" modified="2022-10-12" version="1">MYTRRNGSRLKLNETSKNFLIKRTCSTNKSSISSLNTSNSSKFFQFEDKTYPEDGKLLDLLSENESQQKSQIYHLQMSFFQIHNEESVQQRYALSDDVNNPQLRRNITYNWHYHNDHQQYLFQDTISFSFSIKKHGIKEMLRAKMVDWMIEVFGNYPTTTTTQTYFRAVGLLDAFLKKTSMYYQDADVHLMGISCIFIATKLEDIYHIPLSDIVIRVGHNKFNSSKVKSMEQTILETLQFNVYFPTPLDYLQNLFYKCFSLNDNYTLQNIYETCVYILKMCMHDLHMLNYTPNLLSAAIVGYAVREYIDTKQENKKADNLKLNKQSQDSIVKIAKVDFNTFNECQKQISELINTFKCKYPDLHNLELFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZY1</accession>
    <name>A0A8S1MZY1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0630262</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZY1"/>
    <dbReference type="OMA" id="MELLIFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051019">
        <property type="entry name" value="VLCFA-Steroid_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899:SF13">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8080434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="324" mass="37714" checksum="85F76B597EB8BBFE" modified="2022-10-12" version="1">MELLNIQQLIRLHKEFIHIYYQHLHKRSKLISFFIGVASFYLIKTFAKLLWMTIPRVRRNIIQRYGRNSWAVVTGGSDGVGKEFCIELAKQGFNIVVVAKNEQKMNELSQQLQNFQVETKIIVADFSQEHSIEFYEKVKSDLRYLDISILVNNVGIGEGSLFVQEKMENILKIFRINALSTLMMTKILINKLEFRQNKSAVITVSSGLTYLPCPFVSVYSSTKSFIHYFTQSLGLTYKNTDFISVTPLGVRTKMLAMKQGPEVIEPNLLVKNVLDDLRLGKKISYGCNIHKSMIYYFLWRNKNYRDQIRIRIGNNLKDLYIQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N224</accession>
    <name>A0A8S1N224_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0770027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP97 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N224"/>
    <dbReference type="OMA" id="SAIGYHR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029488">
        <property type="entry name" value="Hmw/CFAP97"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13879">
        <property type="entry name" value="Hmw_CFAP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="178" mass="21487" checksum="5F86C929252DC915" modified="2022-10-12" version="1">MNKSKYLYYTNDSNELLRRDRDFLIHQQKLEEIKRSKSAIGYHRSKSIKLPKSTNIKDEMIQAEIYHKNQLLASNLKRIHARSFQVPQKLQNFQISLKSSQSKEQTFKILDENINLCKRILDQQSQIDFKQKLQEYKQHKKIKFRLQKVKRQFEQQEKLAVDQRFKSKSFSQVQVNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4C6</accession>
    <name>A0A8S1N4C6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0790036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4C6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="45" mass="5527" checksum="F7B0E9792111F6C7" modified="2022-10-12" version="1">MLTSDIYILFYRFMRPSILFQLYQIQNNHIFKTLDKNKGPNLLYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4R5</accession>
    <name>A0A8S1N4R5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4R5"/>
    <dbReference type="OMA" id="TNQFCYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="58321" checksum="B3DC037451C24097" modified="2022-10-12" version="1">MRQWKIKSQILILQIIANLLVAALVAGYLLFVQNLLIKQQEQDQKKMLLQKIEILTTEALAQYARSIEYVNYQSQQSIRSIQEIFLIMQKYNIYSKENSGICEYFKKETNQFCYLIPNQSSQSNISDYLTLTNLLDSLKHQIPVEKLYFVSNQDDQYFSIFPTFILKNYYPHSRPWYQNHQLNQNKSLYVLSDPYISMFSGVMFTETTTLKDQNFIQKGVIGHDYNMSSFSVFNFQIDGIKISLIDMVGRLLISSYYKNFSLEFKFIQNQSLTGLNESNFQEILKYTIQKDYKTQCLPELKQTLCIEINETSHQLIRTQQIKQQILILQIEENVYQEKLNQLISQMQFTVSQQLIYQLYYSIGSLFTLVVMNYIAQILISRPLKRLKILVDYKQHFGKVSKILLKNSEIKSFNWIEQLEHKINEIYQFENSRKKNNQELISIQQIQYPLKVWNYPKTNELIMLLPNNVSNNQFSNLNILFENKQHLINKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4T4</accession>
    <name>A0A8S1N4T4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0670055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4T4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="82" mass="9635" checksum="FF812B46C904C1B6" modified="2022-10-12" version="1">MNYIQIQNRLNFFCITSQRKRNTSQYTQQSQVLVKNKCVQFQKMKQNNLFELLLMDTGTRMPSPISSQISVGIGRIPKDFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCZ5</accession>
    <name>A0A8S1NCZ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0860065</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCZ5"/>
    <dbReference type="OMA" id="QARVWPQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052983">
        <property type="entry name" value="MFS_Riboflavin_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43385">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER RIBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43385:SF1">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER RIBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="477"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8090498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="529" mass="58287" checksum="351361F5A7C1B968" modified="2022-10-12" version="1">MEQPDASSIAQNNLTGLATVHGGNEQNKLLHKLSDQSSPMKTDIQINPNKEIYTEEEMKNFTKRGIISLIGGVILHIELGTFYVWGSISPYVCAWMREKDTGVTLNFMAIIFPILGVITMSVLSFGIKIAEKIGFKVTIGIGSFTIALAFLIISFIQQIGGFIAVYCIMVGISGGLLYMLPIICGWRYFPNRRGLVSGLTIGGYGFGSFIFNFVCKAIANPNNIKPSVIEVEDGKDVKYFDSEVGDNVPLMLQVLAASYFGLGIIATIMVRYPSEIDPDKMLATLEAEEKKKRKDGAPAPAPPVLPAHKECVEVSRGMKHPSFIILQLIVLMSCTFGMLISNCYKYYGLELGIDDATLTATGSVAGVMNGSSRFFWATLTDKTSYKFTFTLISILNLITTAILPYNKDGIGYLLLIAVVYLAEGGLLATYPVICAKIYGKKIGGLMYGFMFFFVGVSNMIGYILYAFARKKIKWEGVFWICFALNVIGIVLGLILKEVGYDWRDQAVIDAEKEKERELQNKGQLAAFQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ND68</accession>
    <name>A0A8S1ND68_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0720218</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ND68"/>
    <dbReference type="OMA" id="WPGQYIP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="133"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="392"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="76"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8084704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="515" mass="60162" checksum="C620E219510BC90C" modified="2022-10-12" version="1">MSDEQNAEQKQFLQDGQQEQSQQQQIDEHTQQPKEDIQQKEDNQEKLEKKEEAVEQMKQDIGVKSDIEQQQEASETPEKIVQKEPESEIIKDENKIEGDQSKGQEDKSQVIEKSPETISSNLENLFLKADYTFPIEIKVCDPVQKGGGISTYIVYTIKGKDCFGEFETCRRFNEFYTIRELLLAKWPGQYIPPIPEKSVSTGTEIIIERTRLLNIFCMKMMQIKHLYYSEEFYDIFLRSTNPDINKQIAQIPKQNVFAYTERYKQAFQINEIKEITPEMIKKMSGVQSFLKLSQASLTKLLEQAKSLSSSRKQLKETFFTIFGLNISEYEKQVLQEWVPQNKLIFANPANAEILELAKKIRDPNNNSLDKIQDLISTEQRDIDAFIQAFQTRDSLMQSKQKAEQKLSEEQSDLYKIMKGNSTMKGSFSKLSLDEQKQKLELQLNETQKEVDQYKLLFQLVTDVLTSYTIDKFKKDKHQTYKSILADLAQCECQYLEIQQQFWFKVSETIKQNQEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NE94</accession>
    <name>A0A8S1NE94_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0780193</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NE94"/>
    <dbReference type="OMA" id="KGTEWDG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035856315" evidence="2">
        <location>
            <begin position="20"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="628"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="595"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="644"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="655"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="666"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="807"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="848"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="885"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="910"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="939"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="972"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1028"/>
            <end position="1072"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8087425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1158" mass="123324" checksum="BA2C60AE142CD2F7" modified="2022-10-12" version="1">MVQVKFIVLLVCALTLTQAFEAEGTLLSMSLNEINNLNLGDVNCQNTSQQQFVQLEQNLESWADIIDHKDDIQKDINTLKQLEVILLQVKKQTQKKKIAPQQLKQINQVVTKKIKSLVLPQTASKIGQAHCDEIERQLERLSSDNESERSECCDALLKLATFLIRQLSSINQQCHQNPVTVIKIKGQIKELHIIQGGCGGGQTIDIPMGGSDEKCDKPETPTKPTDPTTPDTPTDDPVPDPEKEEETTDEETTDEETTTEEGETTPPGPDAEEEGEPTPPGPDAEEEGETTPPGPDAEEEGETTPPGPDAEEEGETTPPGPDAEEEGETTPPGPDAEEEGETTPPGPDAEEEGETTPPGPDAEEEGETTPPGPDAEEEGETTTPSAEEELYEPAEEEEESFEGGEKNNTKGPGDIPDDGEEEGFLPEEEFSPEDGPQSEEEGAATEEGTPAGEEEGTPAGEEASEEEQEQLVPGEEGLPEGEEEESEFPGDIPSNKNGTSTLPDLPEGEEGAEEQGEEGGEEPEKPRSRASRPARREEGEEGEEGTPEGEEGTQEGEEGTPEGEDGEEAGEEEEGWVPGDIPKGKNGTSTLPDLPEGEEGAEEGSEEVGEEITVIKIKGQIKELHIIQGGCGGGQTIDIPMGGSDEKCDKPETPTKPTDPTTPDTPTDDPVPDPEKEEETTDEETTDEETTTEEGETTPPGPDAEEEGEPTPPGPDAEEEGETTPPGPDAEEEGETTPPGPDAEEEGETTPPGPDAEEEGETTPPGPDAEEEGETTPPGPDAEEEGETTPPGPDAEEEGETTPPGPDAEEEGETTTPSAEEELYEPAEEEEESFEGGEKNNTKGPGDIPDDGEEEGFLPEEEFSPEDGPQSEEEGAATEEGTPAGEEEGTPAGEEASEEEQEQLVPGEEGLPEGEEEESEFPGDIPSNKNGTSTLPDLPEGEEGAEEQGEEGGEEPEKPRSRASRPARREEGEEGEEGTPEGEEGTQEGEEGTPEGEDGEEAGEEEEGWVPGDIPKGKNGTSTLPDLPEGEEGAEEGSEEVGEESGEEGGEEGDEIPEEDTEETADQPEEQEEKCQETVEITATNAADIMCALGQYLSQLAHGGSPSDSPNAKKVCFCLKYEPDNADPSLLQLTQKVSKKQRKEQDGILKALIPNRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJ02</accession>
    <name>A0A8S1NJ02_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Isopropylmalate dehydrogenase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0860029</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJ02"/>
    <dbReference type="OMA" id="VRPCRYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000014">
        <property type="entry name" value="Isocitrate dehydrogenase (NAD(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="9">
        <location>
            <begin position="20"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8090426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="348" mass="38269" checksum="DFA57BB68DD31FA0" modified="2022-10-12" version="1">MNIIRKASTTILRPQFNYQKVILFPGDGIGPEISKAVIDIFDAAKVPIEWEFHEIHKKRVTEQGDLITEDTLKQVKALKYALKGPFETPIGKGYRSINVTLRKRLQLFANVRPCKSIKGVKTPYPGVDVVTIRENTEGEYSGLEHEVVPGVVENLKIVSYNACQNIAQYAFEFARANNRKQVVACHKAGVMKQGDGLFLKVCDDVAKNYPEIEFSEEQIDTMAFKLANDPTKIDVMVMPNLYGDIVSDLCAGLIGGLGLTASGNIGKDCEVYEAVHGTAPDIAGKNLANPTALLLSGIMMLKAMKLNDYANRIETATYSVLEEGRFLTGDLGGKSTTTDYTKAIIDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NR41</accession>
    <name>A0A8S1NR41_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0880144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NR41"/>
    <dbReference type="OMA" id="QPRNSQH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8091585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="26239" checksum="2BF266FF54C7E80E" modified="2022-10-12" version="1">MKQYKLDQLAQIIKRLEHILDNAQPRNSQHSLFRLFPTFQPDEQTSSEQSTNQIIFDEQATQDYTKNDLKRFSTESVFIKLDYTDISTQLRSILQNSFKNLNIDSVNHISELFIKHTQKDNPYSALIINQLNLIQSVIEGHSICQKYDTLIQNLSNQFINRDQQELMSQVISIRSNFFQEINKHKIDKTLVPQYDQFQLIKEQQIKIEKTQQAIELLIQKFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NV98</accession>
    <name>A0A8S1NV98_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0930013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NV98"/>
    <dbReference type="OMA" id="NIGMEMF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="390"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8093323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="446" mass="53247" checksum="F68698BCDC50BF98" modified="2022-10-12" version="1">MQQVQKEEIPYFKYCQKLGGVQRKDIYVSPTKAATFLHLIKHRCDKDSALRFAQRRICKWDTTIKQDGPSNCRILKPKENKEKRIHGWQNIGMEMFYENKDTVADINQLELDQDNDTIQKEIEKNEMKLKNDFKNKIAHLQQFYRELNQKYSFSRSDPNFKEKITMAKLDLIHNRNQPIKYLKTKGMKYIELYNEFNQNKKLKGNGIFNKLHVFNQLRTDDCKVKEKLLSELYKQKKENQSVNHKELQHDVHIYDNIEEQSEQMLKIIEQTNDFSMNLRKNEHKLTVSQKAIAFAEDFNRTKYLFNYSSKNQMQKMFEIPEIPQIPQSTQSIKLKKHFSSPSSFDDHFDNCLNQYAHEVLEYQQEMQDKEITTENLQKMLQKRENLRFSQMRKVSKTSHSHQPSIHLDFSKVKCQHQSSRDRESTKAGNSFNIKQRSYLVSPFSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXY6</accession>
    <name>A0A8S1NXY6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0980074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NXY6"/>
    <dbReference type="OMA" id="DHPICTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="349"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="439" mass="52999" checksum="36AC7B83928419DE" modified="2022-10-12" version="1">MKNIDPNVVFVKKDKWDYEQLEPIPCKQIEIPNFVTISTVAQTKIQLPIVYIHTNIVYPDENEYSCDEEDIEAGIDPKVMQQLDLRRHLNSNHNYCKQYSLLNTLEYDETYYNKKVERLQRPLLRVFMRAQENDYRMNVAFRPSKAYKQKRQKHLDIQSIDVAREILLNENMIALNLLRQIKQRELLKLQLIKEEEFNFVNTFNNCNDLFVNLKELKCFYKQEDLIEQQKDNRNKIFESCSNNECSYQFYSMSLSSELIDFMDEQYQQIKDKGVIWPKNPTMLQFWNVDNYTKLGVHQSNKSLIYTTNNRTLMIADRTKKNKKKVKLMLIDRNRASIFDGVRANFDHFYAHYQKINQKQEQIIEQQRKECQKIRFEKQKKEHQLKLQQKKEELKQSLRFKMRIIQDLDHPICTIKIKYSDELKFQLVKDIHYNSQVILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZB8</accession>
    <name>A0A8S1NZB8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0980014</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZB8"/>
    <dbReference type="OMA" id="EIYLPCN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF9">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="124"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8095123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="227" mass="26769" checksum="E215B909F5418796" modified="2022-10-12" version="1">MSDHDDNDNDQELQEETTTNAPKFEDYSDMDKTQIPIFEKNAGTDCFKKGDYFEATRHYAKAVMAYQFFIKDGVVNDPEEIKKMTQEIYLPCNLNLSLCYIKQKEYQMGKDFANKSLEVDANNIKGLYRRGICLMHLQEFKAAGEDFKRILEIDPNNDDAKQAWEQLIKKKEQIKQKQKAISEKMLNSLSYEDKIVTNTKKGKKNWFREKYDWLKQILCVRKKAKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZT5</accession>
    <name>A0A8S1NZT5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1030142</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZT5"/>
    <dbReference type="OMA" id="DGQYWNK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF11">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="93"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8097259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="232" mass="26548" checksum="F9A12AE2755F2784" modified="2022-10-12" version="1">MYQVFISGLPYTASENDVQALFEDCGEILSIKLPRYQDSNRLLGYGHITFNDSDAIQKALALNGSQLGGRYIDVKEAKGTQPQKTGVPPPECRTIFVKNLSYDLNADQIGDSFRPCGKVANVRMVYNTVTKNFKGFAYIDFEDHQSVIKALQMNGKKVHGRQVQVDFDVKNPKAGYRYQPKQIEQQSKYNKEYQEIQKKKIKKEKVVQKVKKIKNFAETQKQISAQRNKQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P757</accession>
    <name>A0A8S1P757_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1080050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P757"/>
    <dbReference type="OMA" id="MSIHSIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="273"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="432" mass="51180" checksum="E03AA153DA4BD0B3" modified="2022-10-12" version="1">MSIHSIQEKQRKQAIYITYKLENVDCLVQLELMSKKVNFLNFSQSYLRLETPFKYFGIEYQESGFITIPIFVQILNQAQKNPIKGASNDVINELIDDFESIEEDLNQRLVHLFKLSNNPEFSDDKSIKENDRLLMSKIREIDQSISDNYKYRNQNWQLLYVVETTAKIIWNGEEKKIIKLGKTNKSIQDYVLKLRKQYQGKIEFRPLFAQRLYTPQNNLVDKIFFHKELLKSIPDSKVYLKADENQSSQIHYEFYFLTNDFVNNFEQCLKKFIEKHKQEYDEVLDQSNDKEDNQGEQTLDLLQDSQIQFQETFEQKQQEQETLKNNLQEISSIQSKEEESINLILQSDNQDEKQTINEPSEISKSLDETSFEFFDKDPIVNQNLLGIPVNYQISEESQEILYEASKMINNQNNYLKALLYIAAAKREMKNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P867</accession>
    <name>A0A8S1P867_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1090101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P867"/>
    <dbReference type="OMA" id="FQTIPKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8099347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="160" mass="19457" checksum="E7D19865F54539E6" modified="2022-10-12" version="1">MEVFQTSNQKQIRTKQNKKQFNLFILGKAQQKKDEQQLKDTFQYYKLKDEYLQYQIHKNTTKGLMLFQNKNINKSFIDASSHVSIEQEQLRTRTPDIRVLKRKQNGKVIFQTIPKMRFPQQQLVQKIDQDLIIQKLQIQTYIMYEYKKGLFFSERNDKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PAK2</accession>
    <name>A0A8S1PAK2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1100118</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PAK2"/>
    <dbReference type="OMA" id="AHWKEDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042175">
        <property type="term" value="C:nuclear outer membrane-endoplasmic reticulum membrane network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039604">
        <property type="entry name" value="Bfr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027:SF2">
        <property type="entry name" value="LEBERCILIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027">
        <property type="entry name" value="NUCLEAR SEGREGATION PROTEIN BFR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="473"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="542"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="489"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="542"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8099743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="578" mass="68911" checksum="0E1845EF9D7A37B5" modified="2022-10-12" version="1">MDPTPQPEVQKEEKKPNQSRGENRKREPKQKQQQVQYVVKGEEGAATEAQNEKQEKKGDQGEKQQKKKQREERKRPEFDWDKTKITLESKVPELPAHPPQKPNKEEHYKQLDKIDEDIRKQWNHFNEFVKNVKEQQNMARQGNEEKYKNEVKPLGLVIGDKIKLVNEKSSELKKMKSENDEIKDHINKLFNQAQEYRQKMKSLDDPDRIIDELARLKESLQQDKLSANEEKRIVQQISALERSLPYIEPLQKLEKEQKVLRTKSAAIGKVMSSLYQSIKDLNEEINQYKSDYEKLKDSTKDSVVTKQNPLIDKERQIVEAKVQELKERKEKLKKDYNNQWDKFEDYEDLQKYREWFLNQINRIKRDAERKKKEEEKKKREEARAKREADREAEEKAEAEAKAKELENVNPFQYQVDLCETLITYCNKLKPTAAQQQAQQQKQIDVEQVLKSDDWKKEKCTVIKKDTDDDIYNFSQLKSKKKNKQPQQQKEAKEEKKDEKPSILQHDLQTLQFFDTVKVATPFYITELDKVVNSIQERKEYYLNRENWGKKEEQEKQAEESKAKKQQKAQNQDEFPALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PB49</accession>
    <name>A0A8S1PB49_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1110063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8099968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PB49"/>
    <dbReference type="OMA" id="VSKNTFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028001">
        <property type="entry name" value="SAXO5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34828">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34828:SF1">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="315"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="459" mass="52793" checksum="C52079C4F04E5A32" modified="2022-10-12" version="1">MQQLRSSHFQLGQTDQKNQFTSTMKMNFKGDQVNDQATGVGDQLNLRGVHFHLGESKPSYNTIFKTDFQGKQIGEPATLNEEKKNDLRTNHFVLGHQQAQQVSITRATYNEKPLQPGLQNEQEIQKNKMRSHHHNFAESSHKMMQTNYNQQYQPQQLEPKQDLAERARQLRVSNIFLGKEQVPMITAQQEDYNKKEGGAQASFKPGFQSTHFNFGNANPDFKTMNQEFFHQQEITSNPFAEENRQNLRATHFTLGKDNQPYQSEQMSHFRPHSENKVNYINNTAALQGSHFTIGDPRFMNHKTQTFYNSTMKSPQQQQNSQARDQQMDRGSNFKVGTDNISYRSEMQSNFKNPSGQPAQLSEKLLKDLRSSHFGLNDNVSKNTFMTTKQMEALNQQQGQPNKLDPHASANLRSHHFNLGQTKGDLISQTHDIHRPLIGKPNTLNQQQANNLRKHHFALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCZ6</accession>
    <name>A0A8S1PCZ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD-40 repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1130016</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCZ6"/>
    <dbReference type="OMA" id="MENIQDC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="218"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="260"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="302"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="344"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="386"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="428"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="470"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="512"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="554"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="596"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="638"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="680"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="722"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="764"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8100623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="894" mass="99365" checksum="13E15C17AA13DE60" modified="2022-10-12" version="1">MNNTDEEKEELFEIFTQVKDVDEQIYCFITQIFRMENIQDCLGFLSDNGNQRHFEQYISQGQNQIQIEKEKKCYFVRNNIIQITDAIKKIMDHHFNKQDYSSKEFSKQRQYLIKRIKDQKQIIEFLKFLIHLTAQDDQLIQLGSNSLYLLVEMKVDLKEQTFEKMRIKNTSLIGANFARCNLSESQFENVDISGMNLNGAQLLNCKWRNIQIHEINKLDGHTWQVNSVCFSPDGFTLASGSEDMSIRLWDFKTGQEKGKLNGHTNHVYSVCFSPDGTTLASCSKDKSIRLWDVKTRKKKAKLNGHSNSVWSVCFSPNGTILASCSDDKSILLWDVKSGQQQTKFDGHSSWVYSVCFSPDGTTLASGGKDNSIRLWDVEKGQQNAKLDGHSGAVCSVCFSPDGSTLASGSEDKSIYLWDVKTRQQKAKLNGHSNWVFSLCISPDGSTLASGSKDNSIRLWDTEKGQSKGKIDGHRGPVQSVCFSPKGTILSSGGGDQFIYLWDVMAGQQNAKLDGHSGAVCSVCFSPDGSTLVSGSEDKAIRFWDVKTGQKKGKIVGHSGTVSSVCFSPDGSKLASGSRDKSIRLWDVKTRQQNVKLDGLSGSIFSLCFSPDGSTLVSGSEDKVISLWDVMTGQQNAKLHGHNGMVCSLCFSPDGSTLASGSRDKSIRLWDVNTGEQKGKLNGHTHYIYSICFTPDSTTLASCSQDKSIRLWDVKTGQQTAQLDGHSCSVNSVCFSPDGTTLTSGSDDKSIRLWDVKTGLQKAKLDGHDGSVLSLCFAPNGTTLASSSIDKSIRLWDIKKEDQILLTNKRYEDILTKFKTTMFKNNYLSQVSNIISSLLISQQPIFQAQGALILKGEFFNNQGIDLRQLFKSKGSCILEIQTEVPKQSNQQYIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PDB8</accession>
    <name>A0A8S1PDB8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1130076</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PDB8"/>
    <dbReference type="OMA" id="TCIVEQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000128">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF511">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="42"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="105"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8100743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="243" mass="28299" checksum="CBA43384952A05B4" modified="2022-10-12" version="1">MSLASDPFSRSIVSTTIPIEDLKFVYSVPQIVDPDPLTCIVEQTHEEGAIYVGGYEGAKDLETLKRLKIRAVLTASQETAVQYSDLIVQFHHVIQAHDKDDYNILQFADQTFDFIERHRKHTNILVHCFLGISRSPTIVAAYLMRKHNMNMEKALWKLKSKRRQVNPNSGFLKQLLNYEKLLQQQQQQQQQQQLQKPKSIFKMVRQQAAPQTQMLPQSRILPMDSRVIFQKQPSQYIPQPYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PF57</accession>
    <name>A0A8S1PF57_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1160137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="RINFMEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1102"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1299"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1407"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1620"/>
            <end position="1644"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1054"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8101797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1687" mass="199907" checksum="8C9862310DA73A07" modified="2022-10-12" version="1">MKQFNQLKNFNYYVASCNILLEINFIKFNNLWLYLIHLISEFQIFGIVVLNLNKELLQLPLMNIDYGLNIISIISRPYKLIVEDNFFKYSYIIPLTLISLYIFHQLITFFLFSNQSQKNIIEYVMKSNNIKLIFHSISIMFLAIFDKLLCIPCIQLVCYSIEQNINNEFFGATEFINILFSILTFILILWIQLLYLMFVKEAITLNLFNFKVLIFQGLDYFYTFIIYIIVIIDCMNLEMKIKNYLIFIMFIILSIFKLNQLLNKYQYVSNIQDYLINLNVAFILISILLIGNSFIEEKQNLIIIIIIVPSLAIALIQQIKKKIDFSMLVKLPENQSTQTQQYLISIFQQKDQLNRIQQFSLSLFLFHHKNNCLEKSCSCKKQGLENEIKLKQLYLKQLILEFGKSINKLTDQSLKGFYCLFYIQSLIAINQSVKAYQQTNILLLKQSVHSMNDQQNIFSEQTTYSMTVEENQNVYKKKSNSDMIHRLEKISLNYISYINKIKINIILEIAKQNLISSFSFGNVIQKTQLSQSVQLFMKVEEQHQQLKHNITNIINRKKDIFMIMSSTTKLNPERFLNQCLDLIRRINFMENELQHLFQEFPSKKMQSIYSFYCAEILNNFMQAYRIINYNTISDNALIKIQRNYSVDLLTTQLHYMILIMDHQGTGLQIVERSHQMHKIIEYDQQEFKEIKSILSLLPKGFSTIHKHLIEDFLITGRSKFFREQNVNLILQRDSFLCAVDFFFDFDLTKLQELTFQVFFSENNGTNSYLILNNKHLILGVTRELCKQLKLEENEQQKLPDQIYLMDIQQLIPEYFTLIDQNNIDKSQPFSNVQIIFNDKNQTIQKSISNDTKKTTEQIRFYYANIVVTIRLTHSIIEIKNVVESKKSIQQESIVDYDNFEQECYDFIEEFHIQQPNQYNNDLFPKLKKGIDIQNDCYIQEEINENIDQIDTNRIISQQQRQLISESIQQYELISPGRSNRQLIDKTNSFATHQINQSCQQRFFQHQNEEKEKEQSQSNQSYAEKGFAKLNNEQNQQNAEIRENLRQQLNEENQTTLQAKKINLDNAASSTLGTFSQNFQLFKKYELLQQIVQSIKFSIMFDLMIFSLLIVSLISIIFAIILINNSSTDIYSALSQMQMLEFYTSFMNPCYLFLSSFSSTYNYMNGSFEHNQLQNIQSLIQYNQYTINQSFINIKKSYSIQSQGNLLQQDLSTTFIDFKYIKGNEFTSEQISLREAIFLIIQYHYNFQSIYTQENGLEQMIQQLISYLINLDDVNKEILNLNTEIITYISDNHFLLQQKWIIVCLRCTYLILLFQLSAWLLYIKHIRKYSKILKLFQKVDIVWVLRDLERCKELLNLLNKDSNLMFRYKFNIFLKERFFKSELSKKHIIQDKIKRTGFIKLDNKQKLLLSRLGSFILYSVLFCIFFIYSFIMNLQGINFMDYYQMKSLQYNQIGEFGISIPKAYSLREVLYFKSNNFIGYEYISYNLTDQYLLIIQNSLDSLSNFLQTYTFTDSNYNDQNLLDLNNQTLCEVESISKTFDLQQQFLCKQIYDNVMDRGLSITITKIRDILLTEMNNSQLFSQRINPPFNEIEIGIYLSVIIMNVLQTIKTELSDQADQLNMTIWIVSIIYLVFTFLKIGFILLFVRTHYLNEFQNIKKITILLPQAALFIDDLFERQLRQLIAKENLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PFS4</accession>
    <name>A0A8S1PFS4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1170086</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PFS4"/>
    <dbReference type="OMA" id="DIDWQDC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000465">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="142"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="175"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="208"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="366"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8102017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="673" mass="78255" checksum="7BB01D4198148722" modified="2022-10-12" version="1">MSKEQKLKRFHQVSESLDQFDLVEQNEFAVPNIQQFSQECYDAFEKAYSKDQPRSIEKISEDNRKFLILNKDTGLQIDCRNLENFEVPVFDLKEVAWKNYWKQSREISEQLLHHVQQNDKDKVFELLSNPCIYIDINIKDLDDWTPLHFACQYNNLEIVQFLLKQEANPKSLSLDKKSPLHIASIKNTYEICELLINYGANLDEQDSDLNTPLHIASKYGNDKVCQILLEKNANHEQKNNQNLTPIELASDINIIEVFNRYGISLNNFTYTRTVIKEQNLILQNSRRDHVERLLKLTQRRSKKQLSNAAEPLDNNEQRISIITSDTRSTWNKIVNFAVSFYRVSIKGNNLNTILEIEQNRLGPQSFQFYQKLGEGGFGEVYLVEKIGQLPKKYYAMKILKKEDINTSNIIKSAQIEKDVLKMMNHPFIVKLNWAFQTSDHLYLVMDLCPGGDLATHLELLNSYPENIVKIFAAEITLALEALHSQGIIFRDLKPENIVLDANGHALLTDFGLSKSGIDEEILNQSFCGTLAYLAPEMLMKKGHGRQVDWYMLGILIYELLVGAPPYYDSEKEVLKENIKKAPLRIPKFLSEEAKDIIIKLLIRNPKKRLGCKEDAKEIKCHPWFNDIDWQDCYNKKLKPPKPLIYHEPKDPRKITFSKISMKNKLEDWSFFEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJB0</accession>
    <name>A0A8S1PJB0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PDEase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1200123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJB0"/>
    <dbReference type="OMA" id="EHFDEYN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="1">
        <location>
            <begin position="33"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="359" mass="42181" checksum="31ED16405F298187" modified="2022-10-12" version="1">MFKPRSTQRQTINYRNSILQFEPAKLGVQHKSSLRLEDLDIQCLVPNIDNFHFNILVVDDMMQKQLVVVVEILKYHNLIKEYDIPLDTLCQFLSEVEYKYNKKNNVFHNYDHGISVMQNVHAILLQLHQTNNSSILSLFNQIALLLSGLCHDVSHTGRTNTFEINSLSNLAIRYHDRSVLEQYHAAKSLKLLCVPNTNILQSLKSEEFRKFRRIFISNILYTDITEHFNLIKNFETKILEQNFGSQDEDIKLLSGMIIHTSDFTGGAKQFELSKQWSFKVNKKFEQQYELEGKLGYPQLPYMKDLNNLSVMAKQEAGFFKFIVRPLWQSMSKYLDNRLQKQVEYLDEKIQKWEEIANSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLC7</accession>
    <name>A0A8S1PLC7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1210169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLC7"/>
    <dbReference type="OMA" id="DNCNSQR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000057">
        <property type="entry name" value="Cyclin N-terminal domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000281">
        <property type="entry name" value="Cyclin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="337"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8103681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="600" mass="70256" checksum="379DDFC13372DF1F" modified="2022-10-12" version="1">MTTSWNPYFIPKENYQQLNNISFVRFDQNSQQPPQYSKSILQHNKIQIQVNPKPEEIKYKRNKKQSSCIPFQINIPQHTSNSTLTKVAQPSLQLILHKPKLNEDNNLKDSAVWERKDYQNPQVTFESFLNQQNVSRNNSIDSNNKTQFKKNHRQYSHVVQGNSGTYFMISNIPQIKNTNNNTSNSNYSNCDNCNSQREVKQIEKLNIPKFLIQDNSSNQLSERTIKKQSPNNNLLKHKKNTRCISMGEQKHLGLTLECDESSITILSDNANSTKSKNIQMNQTFIQNEDDKDSQIGGFSSETLQALMLQEIEYFPNPDYMVSIQTQISPKVRAILMDWMIDVCSVYHMKRDTYYLAVAYVDSYLSKKSIPKVDVQLLGTASMLIASKMEEVEAKHVSEFEKAANHGYTIDQIYQMEIDVCTVLQWHLNLPTINLWIEFYTNQWDNFITDVQKKFRANNTASYKLMLKLQAYIDCCYLDIQTLNYKSRTLVASFMYLILAIEYQQFTKEQIYYEIPKTRKFILVDSQKSFNKLFTEFVQFSFGFNLVDLIEVIKYAAKFIMLDFKFSDTIQDKIIQRHEDLLIYQKYNQSGLLYIESKLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMR2</accession>
    <name>A0A8S1PMR2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1230102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMR2"/>
    <dbReference type="OMA" id="DNAYQIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8104276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="47126" checksum="19A4D1E5CF7532E2" modified="2022-10-12" version="1">MQVENNVVENKKKEENEEETKGVVTMKRRLIASFDLFQFMPVPKTEMVSSRRSLYGSLLMIGCFLAYFIYTLVAFITNNSPTVNQYEEILEALNITLPEIAFGYFFGDPFTKTINDPSIFQFEMFQVTKFQDVSKKDVKTQIPLKSCNPDWIPASNYTILCPNESANMSGALYQTPEFTYPRIQVQYCNQGGNQTCISQQEIDQIAAGGRILLYIRDNSPNFDLTTGKSVESVPYSTYQYFLMPGLYPRTEIFFQLEKYTIKPDFLRRWSNDVRNFLSIQKINSWITNVSVYSSDNAYQIGFRTELTQQISVLTYSTSFDMISIVGAFWGVLFSSFAAYFLIYNKETFYRENKQWEDFGKQIGVRKAADAFNLTEETPHSKVNDSIVFEMTEIHQDNNHSQNEQDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PN93</accession>
    <name>A0A8S1PN93_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">3-hydroxyisobutyrate dehydrogenase</fullName>
            <shortName evidence="8">HIBADH</shortName>
            <ecNumber evidence="3 8">1.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1250085</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17681"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid degradation; L-valine degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIBADH-related family. 3-hydroxyisobutyrate dehydrogenase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.31" evidence="3 8"/>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PN93"/>
    <dbReference type="OMA" id="CPVPHVY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008442">
        <property type="term" value="F:3-hydroxyisobutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000006">
        <property type="entry name" value="3-hydroxyisobutyrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011548">
        <property type="entry name" value="HIBADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01692">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981:SF7">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0101">Branched-chain amino acid catabolism</keyword>
    <keyword evidence="6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="9">
        <location>
            <begin position="13"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyisobutyrate dehydrogenase-like NAD-binding" evidence="10">
        <location>
            <begin position="169"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU910714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8104817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="299" mass="32223" checksum="853C15C5C4053BE3" modified="2022-10-12" version="1">MRKFISAFGFSNTIGFIGLGNMGIGMAHNLAKTRVVYAYDASPSWQSKIESTNIKPVSQIADVAANADTIITMLPNDKIVKSVAQEIFKKQNKTLIDSSTISPYASYDLAKMAQDTKNIYTDAPVSGGVGGAKLGTLTFMVGAEKELYDKISPILKEMGKNIFHCGKIGGGQIAKMCNNMALAIQMISVAEALALGKNMGMDPAMLSSIMSVSSSRCWSVDTYNPAPGVMPNVPSSKDYDGGFMVELMLKDLGIAIEASKKSGTDTQLGQHAQQIYNKLNEHFGKKDFAIVYKELTKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PS45</accession>
    <name>A0A8S1PS45_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1300022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PS45"/>
    <dbReference type="OMA" id="IVITIRL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8106240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="567" mass="67103" checksum="93B2AB81AA5550EB" modified="2022-10-12" version="1">MNKFTLRFPLELEQKYEEATKESSLSHFKSYHPLLIMINLGVAIIQLQQGQLTNGILESIITIIFIIEFPIVCRMKRNKNICSIFVINNIIMTSIQLMVDKNTNNYNNLYVSGCGITMINITLLEHYDFIWNTISLTIVITIRLIYIIYYFKFDGMAIIYIAVSCYLYILMYRKTYVRRALFLQYENEKEIRKILDEIIKDVYIHFKFDQDSFQFELKYANQLAIQRIGIQGNQDLKDFLHNFSIYDSKHQQQQCENQYKSRNINIQHSTGMDSMNVGQYLYEMMVAVKKNSTFLEIMVKDNSTQQLYNVRCFQYSVINKEIIMLMEQRSKIMEENIQLKLKDQEIQLLTKQLIKINIRTKKQLNQLCSIIESSESNPYFKINESTVNAQIISNTKIQMILDAFQIYYYRRETEQLLDIFSLNQIIYKCLTIMNIIAEVEDKHITFESESNESDIITSISNIVYFILQNLLYLFLVQQTHKNVKFTLRNLDKDKIIIFNFYIDGLISLEFLKQNSFIMSMVYKGLQIVGPKAEIITTSDIINNNTQNPYLIQVKIYKNLHNIMKQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUQ1</accession>
    <name>A0A8S1PUQ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LisH domain-containing protein ARMC9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1310152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PUQ1"/>
    <dbReference type="OMA" id="KFAHQVM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097542">
        <property type="term" value="C:ciliary tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040369">
        <property type="entry name" value="ARMC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048957">
        <property type="entry name" value="ARMC9_LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14881">
        <property type="entry name" value="LISH DOMAIN-CONTAINING PROTEIN ARMC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14881:SF4">
        <property type="entry name" value="LISH DOMAIN-CONTAINING PROTEIN ARMC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21051">
        <property type="entry name" value="ARMC9_LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="56172" checksum="0498D7C49DA13384" modified="2022-10-12" version="1">MKSANSNLPNSIQPDQNEINEYIKEYLRYSSFSNTLECFEAEIKSKQVSNKMLNKQQQSKQIGDDAPRLFHLFKSDNTKTKREINLEKEQKAFNKKYQQILQAGRQIFSVSINLLQLLHSLKETAKNENLSETLENYKIQLGKYHKVIINEGKPEGTELITEQVMHEHKTKLFKNFQDKHVDGMIEVLLSLRVNALQIAPELRKNLVYELIRNDVFNIEATEKFDFVVHLLDINNQSLRHAITSLISVISSTLRGVEYLTYNGNMIIIEKIIKILKEQENGSVTQRFCLAILQKASIKDTVIPTYVHNEMIQWIIQLIQKSINTKIHIFCLDFASATLANVIHTPYTLQYLEKQGKFAHQVMEQLLKFMKDQIQVSVLMHVLICLSYLSKENFAKQMEECRFVERISEFVEYYSVINTENEAAEIDKKTVLDLCAHMFHPKDTSLDNSETVELNELKTEDRIREYENEQGELIFECFQDEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PV32</accession>
    <name>A0A8S1PV32_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1320043</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8106894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PV32"/>
    <dbReference type="OMA" id="MIIELLH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000810">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8106894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="275" mass="32205" checksum="D5EB5F115E8BFA2F" modified="2022-10-12" version="1">MNISQEILGQGLTSDVKKVQINDKEYAIKLFKQTRYLSQIEKEINILSQIQHTNVVKFIEGNPEKKFMIIELLHQMDLFDILAKKKMPFQIVSIKYLISQLAATIKYIHNLGFVHRDIKLENILFDKQLEIKLCDFGFAEPLQNQFVVRTLGTEVYLAPELQIDGGINTKDLPITEVFSLGVCAFQLAFVHPPFRQSTKGCGFWRLISNNEWDKYWQKIDKQNLYNEEFRQLIQGMLESDPKKRMTIDQVLEHQFLIGGCKETFQNEIQERMKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PVW9</accession>
    <name>A0A8S1PVW9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1330158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PVW9"/>
    <dbReference type="OMA" id="MCCSSKK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="438" mass="51548" checksum="E3A8A0A78E6B241C" modified="2022-10-12" version="1">MCCSSKKPKSEIDKSQISKPPASVINNNQSQVQNQQESQLVQNQNKPMEVKEVNLMVSQIESPESRQQKLFESKVEGFKEKYAKLQQDSTEVQYNQKSVDQLKAKLLKEQLIDLQFPNNEIKGPMFLDDNISSYKERGAEFANTLREFNGEIRNMRIIHQTPYNSYSFIGHIKDKGLYFIKCWNQVNLTKFQNFLINLGKQQQKGEQYKKYHAFKVDPLKDTDQNSPIGQMYLISTLEDHNLYSFAASTQFNIDQKLQVANQILTYIDQTDLKSSGYGNSQFQKKSKFISPSNILISTQRNRIDVKISDWQQHLKDFNDMIPDIEFKNNNYTSCSDEPDDQRTYLIKLLLLLFFRLNFNQIESFGNLDGTDQLSRFIDIKQINTDSNFYKQSNFNRYIQSKINLIAQPNQEKYQKIWEEMKDKQIQELSFYRLEKREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PY45</accession>
    <name>A0A8S1PY45_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1330161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PY45"/>
    <dbReference type="OMA" id="HMLDSQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="258" mass="29535" checksum="779B45233937C414" modified="2022-10-12" version="1">MMSPIEVFQQMVKSDTQFVNNSTLSSFNTFKQRLSSLGFSSRTNSSFVNETLATMTTIQAPNFNYQSNQTLSKIDEWQRRQDQMVVQMSQQKQKNLYIPNYPPKQSFDLRSRFEQHNAQFLQINMSLDNKFQRSPSLIAHMLDSQIMSDLSEHPIIKSKTKDDEQKKKSNLKTSALKTAEKKSVKIEEKRKSQLETNPEKKPRASKSESKPPKTPDGKLKKQKSNSSISSSASPKKKNSKPGVESVDSFLKQSTKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZ06</accession>
    <name>A0A8S1PZ06_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1360100</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZ06"/>
    <dbReference type="OMA" id="YQTHYEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="134"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8108281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="512" mass="60093" checksum="85E8576E1BFED05A" modified="2022-10-12" version="1">MLQQQLTKLFIKYDESIPQDNILFKTQFFKMSNHKQVTLLLCSDDFIYKISGNNTKVYILKLNYEVRFSVKAEQTSKQIHKQMKGDEFHTQIYKFTLERDDCNSVEFYNFNFQTMYWFDYLRNKLHMLDYQTHYEINKLIGKGSFASVYEAKGKNDQQFYAAKAFYKKQVFQDPKGRDQIENEINVMRKLNHPNLINLHEVFENKAQIYLILDLARGGTLEYALKVMNAPVPFLSAKVIFRQILEGVQYIHDQGIIHRDLKPDNILFRDFIPLKRYGLINVGNNIMISDFGIATPKKQRMAVYQYCGTPGYMAPEVFLTENDNNQTYDEKCDIFSLGCILYYILIGHPLFGQPVKQTNMEMKIDFAQLEKDLNQSHSLTILLQKMLSLNPQDRPSCREILESKIMEVEYGDDGIPVFRDFQRPKSSPPKRMQPLHPDIDRSKRQSVLNPKMIKLMPILEEKQEIRNSGKDRTTIIIKQRNSHFDEERTPAQRVIAARRSSAYIPSSNQNSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZT8</accession>
    <name>A0A8S1PZT8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1380047</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZT8"/>
    <dbReference type="OMA" id="NSIDHAS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="662" mass="76310" checksum="BDA70A7A2DEDCEA5" modified="2022-10-12" version="1">MSNPLQNTNNIPNTEGWNYKCETPSGPLPVGQIYFGLNCFYGSKTVEGGYIMIYDLPPHYQVRVAFKYKSWLILKSINYEQESYLFADGKVIFYETNNFFFFYSGAFSGEFSHTSETLLLEFLQLGDIPAMSTSGFRELTIDLVLCPSGCQSCNSNILASCIIWSVIDYKMKAKDLYNFNNDGWKPLGEKPANIYKCGRETQFYFFGFFDKTTKLEKTVFLQPHYAIQIQFLIIYINFNRQTKPTTTILFNDLTVYEIVELLDVLQLMICNYIPSEQMSSPTGDQVTRVNFQRAHNQPILKITIAQQQASDINTPWGIRDFRILLKKCHSSCLYSCSGPLISDCFSANSILMQFVSFFTDSQLISFDGWNQISVYTDNKICNGQQIIGGVQDISIQKLSYTIFNLPQHSQIFIQFLFYQIDSFQNNEKLYVDVDDVIVYEQQLVYINDLSIGSKLCGNTQQLDTSFQVKVNSIDHASNNAIIKFYVLGTTTVGRWGIRELQIKADMKLVAQSMVKDDKTVISWHRFTDTLQILNCQNLNFKLIYIQKNTILRKKLKDIPNHTRIFIKFEVVVVYQFKFNNSKYLYLQIDGQNVWKQNFLLTSEINICDNSNRSQKFIFEALIDHQSSNCVLDFIADGSGPTESIIYFGIRNVIIQYEKILVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1Z8</accession>
    <name>A0A8S1Q1Z8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1400015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1Z8"/>
    <dbReference type="OMA" id="KTIVRHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="179" mass="21006" checksum="217EC5957D47DF99" modified="2022-10-12" version="1">MIQRLSYADIRSPQINQNNFKQAVKPLRPSKFLNDFESAPKNFIKEKEVYDIVDYAFPRTSRRRAALGNDKVQQKFEIALPSVQNLSIHSVNQLSHREVTADLKKSDVFHFDKKIENELESINRKIKKIINSNKRQVTFQSSLIVIDEQNKISQVRDPYISNDNQIRRKTIVRHKTLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q3D6</accession>
    <name>A0A8S1Q3D6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phosphatidylserine synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T1400013</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q3D6"/>
    <dbReference type="OMA" id="LPNFWEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106245">
        <property type="term" value="F:L-serine-phosphatidylethanolamine phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006659">
        <property type="term" value="P:phosphatidylserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004277">
        <property type="entry name" value="PSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF7">
        <property type="entry name" value="PHOSPHATIDYLSERINE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03034">
        <property type="entry name" value="PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="360"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8109178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="50980" checksum="F6BB5E170755F338" modified="2022-10-12" version="1">MEIQPKKIDIGIQCEVLQREYIPPNIQSTFFHESEGWSIFYRSRTISLLIFMLTTLYITSYWFQRETLQDSAYIGYIFSFLAFCAYGGSYFPNTLIQRPHPLFWRLLQSWACVYMMSIVFLIYFTPEQLQQFLQQFVDPKLGKPLPQKSYAEDCRFYTPENEHSKFYNLTSSFDVFVPAHIFGWTVKMWILRDFKLAMFQQIAFEFMEITFRHWLPNFWECWWDHVILDIIVCNTGGIVIGWYTMKLFGMKEYKWSLRKENRKDSWFKNFKRLCISPQLDKIEWKVFSSVKRFLLVIWFLVFMNLCDLSFFFNKFVLQINTAHNVMKFRVFPLGFFSIVAARDYYVYISDENVTRLGPNAWLMHAIVLGEWVLFFRNYQGIFVEKFPTYIIIIWIGIFTLFFITVFSIWRRERRLNKLKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q453</accession>
    <name>A0A8S1Q453_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1440039</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q453"/>
    <dbReference type="OMA" id="CEGSISQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="195"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8110419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="470" mass="56316" checksum="B02FF500AA823533" modified="2022-10-12" version="1">MLNNCDELHILRLPKIVNTFAKDEFLQLSMKVSKINEYGNKHTRYFVLTNKKILLLNKSTIRRKVLLSFIIGITISSKSFEFILHFKNEYDDDDLRLQTESRSRLIEFIVKTYALEFHIPLASYQVQDLNLKKYVVYKQDRHKHICKLPNVKANFLNYKPILEEQENFLKMQQIYQQDTESKLLFGEFLLQLKDFELKKILCEGSISQIVQILNSKTHTYHIMKCIKKEDHSQLNLNEFVTFKKYSLHPFLIPLEYCFETFDRYYFIMPQAKCDLYKKLDELKKFDQKQLLFIAQELVVALGDLHNKQIIHGDLTLENILLDQSYHIALCDFGYLRRALKRKDEFYGVAEYTPPETIQNKDYQYYSDYWQLGIILYEMAYGHPPFIDYDQNLTFDYILNCQLEFPIQIEVDPLLKDLLSKLLCKEPTKRIGYQMGISEIQDHEYFKNTNWKQVYDRQLQPPEFEKKLKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QAI1</accession>
    <name>A0A8S1QAI1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1520117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QAI1"/>
    <dbReference type="OMA" id="FHIKKQR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="85" mass="10400" checksum="E59181F4B087AD8E" modified="2022-10-12" version="1">MMNESFYRICFQTLITLCELKLITNQEKLYLKKIVVHQQFQIPENLDINQLSLFFLYYIKKQRRQLEIKNNELLIIDEETDEEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QB71</accession>
    <name>A0A8S1QB71_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1510075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000156">
        <property type="protein sequence ID" value="CAD8112435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QB71"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8112435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="47" mass="5334" checksum="EECDD5541D2CA683" modified="2022-10-12" version="1">MLYATNSMYYRQFLTTTGKKSKAQYQPQHQQTIAKGQNSEHPAAQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCM7</accession>
    <name>A0A8S1QCM7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">60S ribosomal protein L7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1530005</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCM7"/>
    <dbReference type="OMA" id="SYYVDAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005998">
        <property type="entry name" value="Ribosomal_uL30_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012988">
        <property type="entry name" value="Ribosomal_uL30_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01310">
        <property type="entry name" value="uL30_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08079">
        <property type="entry name" value="Ribosomal_L30_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30 N-terminal eukaryotes" evidence="7">
        <location>
            <begin position="14"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="6">
        <location>
            <begin position="91"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8112814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="28678" checksum="D36A28F81326B7F9" modified="2022-10-12" version="1">MSQKKAQIQVEQKVPESIVKKTQRDSKLRDLVVKRRAERLGQNKARRAQLEKNAQAYEAEYKATEKSLIDNIRKAKTEGGFYVPAEAKLMLIIRIRGINTLNPQVRQTLRLLKLRQLHNAAFVRINKATIEMIRKVEPYITYGYPSRTVIKNLIYKRGYAKVNGQRIPLTSNTIIDQQLGKYGIHGIEDLIHEITTVGPHFKEANRFLWAFKLRGPRGGFIAKRKSFINQGDWGNREDLINDLAKRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDA1</accession>
    <name>A0A8S1QDA1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1540036</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDA1"/>
    <dbReference type="OMA" id="HLLCENA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="150"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8113104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="501" mass="59171" checksum="DF8885970E1FC47A" modified="2022-10-12" version="1">MRQQNQKNTFLKTIPYTSIFMEALRQSSMWVQSNTIDGNNQFQGRLLLKVDSQWESVQLNLHPEKLYRKGVGSEDYTSYCQLQACFLKKFTHFDPTMPNYRYGIRLQFGQQLTHLLCENADIFKKWFQFLRRFCIMDKFARKYKVLGKIKHSDPVFGQCLFSCVRVQDAEYHVVKVIEKDVIMAAQKQNLFRELSNLRKLSHTQICYIVEVFEDEQNVYILYEYYLGIDLRSYIKESQIYQMAEQSTQGLEEKLVADIIYGILQAIQHMHSKGVFHRDIKMENLFIPEKKRLPFVVLGNFCYSETTDQSQYKKCGTPGFVAPEIFRSKNYTSKVDLFSLGVIFYLLIYGKLPFEGKDQEEILRSNEKCEIDFKIEKKICKKISNSGMDLLKGLLNKEPIKRLSAVQALNHHWFIKMGTRHQRNQYLQVQRGKSLSTIIENSVDITQSYYQSNSQLHQSTKEDDRLDRVEKEFIQGSLYDKLLHFNSIQYQPSKIRHNQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDS5</accession>
    <name>A0A8S1QDS5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trichohyalin-plectin-homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1570020</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000162">
        <property type="protein sequence ID" value="CAD8113848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDS5"/>
    <dbReference type="OMA" id="CAMNIRR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039986">
        <property type="entry name" value="CFAP210"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043597">
        <property type="entry name" value="TPH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28663:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA- ASSOCIATED PROTEIN 210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28663">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13868">
        <property type="entry name" value="TPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichohyalin-plectin-homology" evidence="3">
        <location>
            <begin position="109"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="138"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8113848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="470" mass="57513" checksum="ACE4423BA7369A4D" modified="2022-10-12" version="1">MLDGRAATSGNFNNKTTSIIPLSQLRRMRNSSCEIQDNHTMIEKQDLHAKSQERVKKWPCAMNIRRRKNQSQFERFAEDEEERRRIEKEEAEYQQKQKQDVLLRANRLIYENNPFIRQFQQKLLFSDVLQERDSQLQLNEYKKTMNNVRDKLYNEEQLEIMANYDQTEKLKEEELKRKKQEQKNVLKQQHDEMINNHIKQMQNEKIEGQLIKARAQKLIQEEEEAEKERKRKRYENMLEIKKGNEEIKEYKLQQLQKDKEQEEQIKLHGEKKDRILEMRKQREQIKFKEKQEQRQKLIDAQIAILMKKEAEQLQLMNKQIKEAEQKDEEVEKLKLIKQEAQKKKIEDSRNIIKEFKLKDKIIKNQNDKEFQQYWKQRGEELQKIEQEDLDNQRKRNQSVKDYQLQQIEQKQKIKEQQILQELQQDEDIKRQQHLENKTFFTWAERAVKEWADQGKNIRPLIKELAKLKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF40</accession>
    <name>A0A8S1QF40_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1540085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF40"/>
    <dbReference type="OMA" id="EYQIRFH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="274" mass="32597" checksum="5E2691DEA944C85B" modified="2022-10-12" version="1">MREFNPYKKRSGSIQNNEPDLLFHINLKSRRKSCTCNQCGKDSEYQIRFHDVQILQWTQIQNNNELDNSTQQEIKRLEQLENRLKTYQEDFEKFDLMSKKRRKSCYCSECGNLTQYEVRNQNIPLFKPITIKNSKNSRKVHQLISNINMQTKSQFFRNFKQQNHALMYQFNNQTSKTTTRLLKQSKLKLPNFDTRLSLTPKLQNSPLSEQSSPQQLKMNSFDNTLNAVYLNPYLGSPKKFLSPPRNFAPKLPPITYKIKDEQLSQRRSLMRKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF59</accession>
    <name>A0A8S1QF59_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1590020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000164">
        <property type="protein sequence ID" value="CAD8114252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="507"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="541"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="834" mass="100223" checksum="D400034DC3317822" modified="2022-10-12" version="1">MCLRPFRCCCFISEPHLDWSPHEMINLKSDFQELIENRLIEVKGCLSILKYYAKIGFQIFLYLLSVLELIFTLIYILHNNIADDTTYFEFCYNLSIISSVVVHILAWMYTYYYKEIDKHSFSVEITNYLYYMFMGLLSYCKLAPFIIYYNRDQSIKQFSFSQINKYQILNYEDKQRNPCRLFKDRKKPVNIFRDIIFHRVALLSMIIMITFQTIPQLIIQGLFNQLKGSWDGFNIICYGLLGINFINFFFELQFILYSTSNSQLQTDINYTFTLKDIELKFIQETKQLIQSNFKYLAFVKSCYFHINPSKFSPYQKKRCMFQIIGFLLKQKQYQNIQFHFIDKYEQDTLQYLANCFKFINVDKICLLYHEENSLILLKGIFKSIPNLKFYPQALQYFDYLWLVKDAYEEQNNKQEIQFIGNPKVTVGWQEEQKSQFIKNEYITISKEFQELISNFKKRRQSDLNKIFNQANDKINAYDKTDFFLESVGDNYCLYKLYRFYHYKWKKLYCYQNYITTAWDFILYVLSFVYVSVYDNTFTKAILVLAIMNAIYQILSYTIFQQQVFKAFSIQTQLSLVLIFTLSACVQTWEVFERILKKDLKHFAGVNRYFGSQTCNKFKYYASKLKGNVANQIFNFEDQFQIKDDLKISPQFEAIMWRINTEDVYNKIAQIFLYILSLQYDHLDSLWAFAFIRLLLDTIERLIDFLEVIILDYLIPALILSKVSVDQFYSSMFYLNTISKQIILEHPKSFSIISKVELYHSQVHNCKIXRYRIKIHLRNKIINIIQFQIFGICQIMLFSYQSKQIQPLSKEKMYVLNNRFLIKVKAILKYLISFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJ08</accession>
    <name>A0A8S1QJ08_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1650084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJ08"/>
    <dbReference type="OMA" id="EYMEAMF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="58"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8115642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="562" mass="66236" checksum="2215E98FE60356FC" modified="2022-10-12" version="1">MNNNQTSHEIQSCKHGKKSFSITSLQADPNKLRNNKILSNNIQISRGDSMSRMSYVDKEDYIDQIQKLKKENNLLRIELKQVKSQLMYYQKELDSLQSQCQESDNISVPLQQQVKIKQLLKTLQQKETEIDQLKKLLKVDQLNEQQEQLQKLRALCEIQKQKLLNSETEIIIPTKTNILRNLEEDNIRLVQIVTALEEKLKGFDSIKKQNTIFQSKIQNQQKLILQLQLEIRQYKDRDTIFANKVTQQNKQDNVIQKLTTEINKLSQSSQNQQSQIDNLMSNLQNQQDKYEKIIMEKEEEILTLRIKIENINKIMIEDHQGLSEQRNRKNSKNTPLFKPSSFANISGNFKLNQDFVLSSQRSQSATHRKKLVLITKQDLQQVIKILKYQLLINQINLNDIDQHLFCSILQDDDIQLKDLVESIQKSIFKLNYQQAHQLSMYLMDSEDGNETSSKQRIRSIFKTLVDNYQVPSTQEIANIKKELSQIFNEQEFQMIKSQLIVKYGHQIKKFQIYDLFEILQLKDIKLSKQIKEYMEAMFFKLNDQTPNVFQIDQLQLIYSNDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QK42</accession>
    <name>A0A8S1QK42_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1650033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QK42"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8115554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="149" mass="17279" checksum="5BFC0D16F1B63777" modified="2022-10-12" version="1">MSINLSGIMALSLLNQQNSLNLKCQNLCIYINYIILNQTQFSSQSSSYLLSKSIIDFKQSSSFKEQEKNKITNKAHINISPRKMAIDLITLLSQNIIPQADNRILSNIRKKTFTKHPHHTKPRKTDQLRILEKIMDIFTYQQMLIRQNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QK54</accession>
    <name>A0A8S1QK54_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1710062</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000180">
        <property type="protein sequence ID" value="CAD8116398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2922"/>
            <end position="2944"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2976"/>
            <end position="2996"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3016"/>
            <end position="3035"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3047"/>
            <end position="3064"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3076"/>
            <end position="3096"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3121"/>
            <end position="3142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3197"/>
            <end position="3214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3234"/>
            <end position="3250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3262"/>
            <end position="3286"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="425"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="610"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1000"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1193"/>
            <end position="1231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8116398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3433" mass="400408" checksum="96F3D68E0F3B4E91" modified="2022-10-12" version="1">MLQNDQYSNNQTLPNEFVQGQINQGFGVWMNYQPFTYKQSRWDKLLPNNTQYQQNHIKGGQFIYSIEENVEKSNWIVVSSEIDSIQQKITHKVFYSFKTSKNTLSFQFDNLLYEGQWIMFYCYFDNFVRQAQIGFYNSKEALSIQVVNDLPEYKQYIKHIVGNFYFYKNQDENLIMLNIFQGLLTSDFSAKQDNVFLDLESCSQYFFSQIYCYDSSEMLIEQNQEFNGNEYQNFISSRLQVPRYVFKGWIILKQSNQDFLESTVFRITVNSKYYNDFQVGDRDLLLQYYQSNIPAQNGFKLTTYSYQFPIYQRYKTKEDDEIKQFGDEYSGLLIQWHYFQYEIGTLNNDGQPIFSIFFPSIDQIRQFNWNKKIYHFSGTSFITYFGGDDFTQNYFRGNVSNLQFKRLCKPEIEIFMPTCHYSCLTCDGPTINNCLTCYEQSFRSFSKKEKICACKQSYVDLNGVQECQEVTKVFPQIQIQEIELNCQHPGYSICNQDNIECLFGYFKYNNKCVQCPNYFDIFSGIQINCLDCLERPNLFGKTLTCKIRAETCEQNPEYIYKITQRSENENIVYNILTGLNESYELKLCHGCASQDTCHKSFYRDDQQCKPCIEGCEICENSFKCTSCFETYYLNEDKQCVQCKDCYTCYTSDQGEYVCYTCQTNLIRLNNKCVTCGHNCQSCXYYNIIKVIYLPRMALSQQLIHISFLYIRDTRQKKMMKQNNLETSILGCQSNGIIFNMKQEHQIMMDNQYFLSFSLLLIKFANLIGIKKYIISQELRLLHILEVMILLKIIFEEMQVIYSLNDYVNQKLKFSCLHVIILAQPVMDQQSITVLLVTSKVLDHFQKKKKYVLVNKVMQILMVFKNAKKQQKFSLKYKYKKQNQTVNTLVIAFAIRIIQNAFLAISNTIISVFNGIQINCLDCLERPNLFGKTLTCKIRAETCEQNPEYIYKITQRSENENIVYNILTGLNESYELKLCHGCASQDTCHKSFYRDDQQCKPCIEGCEICENSFKCTSCFETYYLNEDKQCVQCKDCYTCYTSDQGEYVCYTCQTNLIRLNNKCVTCGHNCQSCYEERLCNYCKGSPSEYYLSFDGINCNQCNIKNCIYCFDYILNDGLFQTNLDLNFKRIDQKTQKVNVGCALCKENYYYNFNNQECELKQIDDDCIFGIIQDIDQKKVCLISNTDQNAVQVSDCSIISNCISCIHNYLENESFCMLCEDGYYSSILTGQCIQCGKKCKTCIQQQEQFRDYWKWNIKAFYKFVINHNNDHPFEKYASSDNEYDLNLVCTSCHYGYILSGQKCFKGCDSTCNKCEIINEQATCIQCLETVSGFTKSYELDGSCRKCPSNCKACSDRTYEEIEELNPQFFLNENNYYKTRICYEKSTNHFGQEKYYQDSLTQTVVVCTKYDKCVNKIIIQINAYCDSYDYYTQKQQSNDEFFDHKNILLSQYGFYEYLSELQTVQLFDYLNYISVKYVQFEYTIIQGEYSNCRIRGDQTFDTRLAQNIFSIQQMDIQFIGKILESSNPITLQTPLELFLSNYTNITFKNINFDFGFDDYSPDNYLIIAIDNKKKTNLNLIDCNFIKTSSFKTHQFLRINSSNPYSLYIKNLQISNLNVSQSEIFTFLSTKALLQNQIQISNMTIKNSIFTNSTLFHFQADTGHLRYESNLINISIDDSKFISSKFFSSSSQLNFTLGTLNIQKLSLNNVKILDHSRIFLLPIVEQATISQLIFTNSQLKDNSYFYSSNVINLKDCLFNHILLESSCLINNKVEYSKNLAALNWSQKIFIINCQFLNINQNDKQQIIIIVKYDEIDQLIIQLTNFIMSDCTSNTKLQNTQVSFDQVMIYIECQLCYLQEIQIQRSYGIPEITILNSKNLEIRNFQISQNQKHFPQVFHSNIDCVSQFANMELYFFLYVGQYESIVFDNLTVSNCLSFNSPLIILQGYELMQKLVEETVYIKDSQLNSNLLIISETNKQTALIFIESKQKGLITFMNTIFSQNHLNQYYESLSLVSSTTLLLSCKYEIVIMQSCQFYQNIITNSTDSILQIDSEKLYIKNSNFQNNNIMNYSILSKQIILSSTQDITAINFQLIFPIKSNSGNGMLIANTILIENVKVDSSNSIFGGGFYIVVFGQSVINITNSQFSNTKSTLSSLYFSKGGCFYIDASSSTLTLKIKNVTFDGSYSRYDGGAIYLSPSETYNNIEFDYLIVKNCFSIQNQFFSYTLSNKDNIKSQITFSNIDFYSTQEGLENFYSQLDSITDDDISNIINSNPLVFVQFGNFSMQNCSFQSIYMQMLLKIVQVEHIQLSNIIIINCTVALSSLIQLNLRQQYVGILEIFNLQITQIKQIQKTEIRECLVINRTSFIQLDCPLEILPQNFQIIQKDLIQENLNQLICNQIKIFRDPKFTFSLIEIEQINSRHYLRIEKLNFKNVLCTKCQNGLIRIVEIEKQNEDNIKLSQIMIQNSNCGRVGCLSISKNKNDLTNLESDINYRILQQHNYENLLNSLKYQVIIKQSLFQNNTSLYGGSLLIIRINTIIKGCIFQNNIADIGGAIYYSSQDEELYIFDSKINENTAKIAGGLYLNSQQLQLTKQLDLELNSNNSTLFGSNVVEKPRSLTLSIDEGRTLLEKKELKYNDNEIVEQIIIIPYNILGFNSKMNQLMLPSGIQISSYRHFDNAQFLFIQYNLTLRIIALDKFQKQIMGLSGSYCTLQPKAINLSSQREEFNVEYSLSQYEVQFNETTGDYNLDDLIIFFNPTYDKDIILQLQIQCNSVSVPQYQENPPYKIYNYITNYKLLVDIRTFPCQLGEFLNKTSGGCVLCDTFKNQYQVSRNAQNCSYKDDTKIKSLESSMIELREHNWRAYYYSETIENCYHLLENCKGGWIPGDESCILGHIGALCEQCDLYNSRGGGSYSVSSVYSCGSCDDIAYNIISIILLSLWTIITTLITVSSTTQMIKEFVKGLRLKAFGVRVAIKEAQAAIFIKVFTNYLQIISTITTFQLQIPIGFSSVFNSVGNPVNTLAYSLDCFLINITDILIIYFRIIWSLIMTSTYISIIFSLFGIAIIIKITKFNFSFITTSLIYLFIFFQPNLVGGIISLFSYRKISDEYWIQGNVAYRYDTNQHTQWLITFCLPLLLIFGTILPFFLWHGVYKNRDRLDFSQVRKTWGYLYNEYGKHAYFWETIKIIQKEIIIIVLTYYDDHISIKASLVFLVLFSYSLITIKQRPYMTGQLNQIDTESTVVCAVSIILASSIFTAQQQKFYEIVWPFYFIIIFLNSFYILKMVIQIIFAYLKKHYEKVDVLKEQIVQQFPKFVNSKPYIQKLFETHKIQQSRIKQQYKKLRLYLLPQARLILEFKKLKRIELSSKKNIQKRNDKDRHDSEQQFSQDNFCVKSFLNKKILNHNHIKRDEFQYRNTKIGYHQFLILSQGDDFQKNQKTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QM50</accession>
    <name>A0A8S1QM50_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1740002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000183">
        <property type="protein sequence ID" value="CAD8116516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QM50"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="86" mass="10098" checksum="4E8733E6CB9CB1AE" modified="2022-10-12" version="1">MRILRGPIPIQNVAIQQSSQGDTGQKNTIQERRLYEDLVLDQKCINLKDIIKLYHNKFYTLQLFGIIMKQSALRGQNYWTLYGLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QN89</accession>
    <name>A0A8S1QN89_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1690037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000178">
        <property type="protein sequence ID" value="CAD8116115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QN89"/>
    <dbReference type="OMA" id="ESCEPFR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050935">
        <property type="entry name" value="Bromo_chromatin_reader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880:SF225">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN BET-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880">
        <property type="entry name" value="FALZ-RELATED BROMODOMAIN-CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="2">
        <location>
            <begin position="25"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8116115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="25839" checksum="70E3ADDF34E6EEC5" modified="2022-10-12" version="1">MIKTTQTPLNKEEQRRLNQIVQQLLDSNDSVEFRQPVDYKALNLHDYITIVKKPMDLGTVQRKLNSNTYKTVEECLDDIQLIWDNCKLYNGSQSWITKIAEKLERLFKKNVRNYLPLVNLPQFVPKQKEAGAGADVFQEEPQDQVSYNDKVEFSNNLKQLAPEQIGLIVHMIQNTSPNAFIEIEGEKYQIIVDYIEYEAFVKCQKQIQTWIAGDSINKKVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNN4</accession>
    <name>A0A8S1QNN4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1710003</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000180">
        <property type="protein sequence ID" value="CAD8116295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053235">
        <property type="entry name" value="Ser_Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24361">
        <property type="entry name" value="MITOGEN-ACTIVATED KINASE KINASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24361:SF433">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="75"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8116295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="565" mass="66284" checksum="9AFB5C690BB34D84" modified="2022-10-12" version="1">MNNNLGILNNRFHKKAIQSRKRQTSADTNTIHQMQGGKKKXDDDDEDDEEEELDSNDDSENEEQQFESNEYEEYIQGDKLVDNKSMYSGFLVNRIQLLAIKKIVLNKKENFKIIKYNLQQLTNLRHQNLETIISWNFENSNNKKNYYLNIFSNFQSNGSLQSILLKYCKFSKELILTIFQSILKALEYLHSQNKLHKNLKTQNILVDHEGTILISDILIQSQYSLSNYSAPETFVYADYSEASDIWSAGCIFYELLTQKQPWQINDKVLSIQEIKKKFSLNQTFLKENLDIQKNAMNILQQIFQFQPENRPSASQLLKHQIFLENSTSKFKQMAKQISQKDVQNTPQKKFMSLIQDCKINNNSNNIQLQPVQYIKLKKKEDDEISFDIKECVFYIQSNILKEFTITQNSNSYLLTKNNFDSLTIKQHKFNCNLIQQKLCHNKQQYRIQDASNLSQIVSQMKTAINFQGSILQSQNSTSEKNFDQKSFQANHLILQTQSFNQLNQEIKQFQQIQDNQNDIKQKNEQIIKQLDLIKSQTQTESTINQNINSQETEQILFDGDFVFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQE1</accession>
    <name>A0A8S1QQE1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1220176</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T2060011</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8104071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000215">
        <property type="protein sequence ID" value="CAD8117671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQE1"/>
    <dbReference type="OMA" id="GGAFWIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053100">
        <property type="entry name" value="Cytochrome_b5-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16740">
        <property type="entry name" value="CYTOCHROME B5-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16740:SF1">
        <property type="entry name" value="CYTOCHROME B5-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="2">
        <location>
            <begin position="40"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8104071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8117671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="417" mass="49641" checksum="ABA8DCBA518A403F" modified="2022-10-12" version="1">MIQSLPSRNKQLIESPFVWLKEKAERDRQRTGVPSKLWYIKGKIYDLSQFLQLHPGGDYFLSLTQGQDITEMYYSHHLNMEKCNAILQKYYVRDADKIDQYFDYDEKGFYLTLHKKVSDYFKTRDKGPTLMMKLVVFIALFSFIITFLLTCTLQSYLWAIICGYTLYVCFGLGHNFLHQGSFEPRRYLADLSFFSHFHWAITHCISHHHFTNSNIDLEVTSFEPYYKTQKSKLPNNKYFRYYMDVFFALVSTIQFLSTIQQLLRGEERIRKEYFIPIVEYLILYYFCGDALEGFKLFMVIQSTSSYLTLKYSLITHHNNICYTDGCSIVPSRDFGIYTLQTSQDHDPQVIFPFNMFIFQGFNQHTLHHMFPTVDESRIPEIQHFLLETIQEFKLQHIYKQRTVGELYKAHNDFVFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQF2</accession>
    <name>A0A8S1QQF2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2180001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000227">
        <property type="protein sequence ID" value="CAD8117959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQF2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="189" mass="22399" checksum="082354E563D53800" modified="2022-10-12" version="1">MKYLVLTFYLSRLIIFRLRLSRFSTRSLKKMTQICQKDKIFGGLQYELKLNFKGFIIITFYILYGPEFSSDGLVEKNEPVSKSTKSYNNFYSNGEKYRRVMKEQIIIKIYQQQVKNASDQIKAYCGFKNYYVAVHKCQPYCLQCLNETTCTQWSSDYVANVVKFSQKECLIHQYYDNDSYRCLNCPLSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QS58</accession>
    <name>A0A8S1QS58_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T2470002</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000256">
        <property type="protein sequence ID" value="CAD8118559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8118559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="712" mass="83249" checksum="DD232B37F8BF93DD" modified="2022-10-12" version="1">MELLNPSEKILLKTYSQMSQSDIEKTKQLTQYQEAMIIAQGIGSVIVLISGNFEIFVEILDNLQYQSYLKYINIIYPENLYLFFESTNLISIIPILDYLQINGFYQRFLYLEFIESYAKLKFYNLNAHLITNLQFFFIQIISTFIFVKTIQTISKISFKLIYKIQTQHFLIFKLRSLKSKFLIKILYWIYNFFHYILTFLYEFNQNGAKDLLLANSWDLLFKTFXISCIRSDINENQISDVTYCDDYDITYNLEISGIKQQNIVFQCPQYCEICEYGKCKKCLVDYFLLNNLCITQITKGALLFKDELQLSLIEIGCYKCNDSCQIQCLQCLNSQCLYCIDGWSLMNGICLQICGDNQIAIWSYEQCDTNLNDCLNCKFLCPENCQYCKNPQTCLICDQPYIEINYQCQLTCRYGCKKCINGICYDNCPNGEMNIDGICYSICGDGIKQQKEQCDDGNKIQFDGCFNCEFSCPQYCENCFEGICIECQQYFKLINNACYDDCGSGIKSYDEQCDDGNLVDVDGCTSNCKIEVNYKCQDKANSYSDCQYSQSPQMLIKFLNQTYNKYYLEIVFSQAIQFRNNYVLESLFNFSIDEALYEDYQIKLESNYEPVINQLLNFSFQIQIELLISTNSSLVQFNVVIMNEVYNSNEMELLNPSEKILLKTYSQMSQSDIEKTIQHKGQEVQLYQFLEILKLLLKKVSILSKLHYYNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QTD8</accession>
    <name>A0A8S1QTD8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2150008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000224">
        <property type="protein sequence ID" value="CAD8117897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QTD8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="408" mass="47734" checksum="CACB661FFF3FE40F" modified="2022-10-12" version="1">MKRGQYEFMIIGGGIFDKNGFKQGKWMELHENFDNNLLVTYYGDYEFGKKIGRWDTLLLQVDESIDNSDCYSESSSQNDNSVDVSMKQTFIILSNSFQQNNLQNQNQIEEVDDQKEGIDFILYCQKEFNIIGGGQFDKDQKKHGFWIENYFDKSLNGTIVKGNYNQGIRVGDWMSFSYNNQNKKIGGGYFDQNGIKQGQWLELRSYFKNNCLIDFGDYKDGIKIGRWDIVQQIDNTPQYSIIGGGFYDLNGQKYGEWIDIQKPSFSYYLITIFKGEYKNGKKFDNWDIKQSSKQCEIFKPICGGQFDIDGQKQGWWLEYCNNQKFKQIIFIGQYRNGLKIGKWEQMNIDEDSSNFQIRYAGLFDENGLKNGVWYLNSKSYQSNTYKVQYKKGFRISACKKNLQSISLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QYU3</accession>
    <name>A0A8S1QYU3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T3980001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000407">
        <property type="protein sequence ID" value="CAD8119982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QYU3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8119982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="424" mass="49114" checksum="5158F960F5B46ACE" modified="2022-10-12" version="1">MTHLKLLIRVNIKRVEKLVDGIFISVIKEQINRCIIILFIIKNIFYSGGGEYDERDEGIKTGKWIEMSEGFYESQQVMFIGDYKNGNKVDKWDIYFNNKGQNEKIGGGQYDEGGEGTKIGRWIEISDDFDDSSQVTYNGEYKKGRKIGRWDIHFKITGRNEQIRGQQNNEGSDRTKIARKIISDKNSKKAEKQDIPDIKVGNEQIGGSYDERGEELKIGKWVELSDSNSQITQIGEYKKGQKVGRWDIYFNNGVNNRMQNYQIKNDLQMIQWCEINTQLTYIGEYKKGQKVGRWDTYFNYFGNKQIGGGLYDEEGEGIKIGRWIEVNDEFFDSSQVTLHGEYKNGQKVGRWDIYFNYGVNKKIGGGSYDQVSVGIKIGKWIEIKDNFKWALQTTLNGEYENGKKIGTWIEMQGNIKSKEIIFEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNS6</accession>
    <name>A0A8S1JNS6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0050549</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNS6"/>
    <dbReference type="OMA" id="LPSMTWQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13990">
        <property type="entry name" value="STKc_TLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000698">
        <property type="entry name" value="Serine/threonine-protein kinase tousled-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF23">
        <property type="entry name" value="TOUSLED-LIKE KINASE, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="337"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="31"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="246"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8043894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="613" mass="72008" checksum="5F40FD05DA57E6BF" modified="2022-10-12" version="1">MHQQLPPEQLDRLICLEARIHKKSKEEIIQEQYLQTEKNKQNDENDNNQPNTDSNKKHEDLKCSNQSEISPDNIVISQVMSPVHKKTNYVTKNSQQKKKKKTKETQQKDITQYLQAQKKQLKVTKGEQKLIEYTIQDSPFKNKTTLPNDPAIEQQLKEKHNKIVIQEIRIKELETLNCELKETISTLTDQQSKMQEVLKLLAIDNEKLKKQQIQIQIKNNRFRLGEFNLQREKINVIDVWIEGQEIKDAKEQAKNLENLKQDLENKKKNLKNKDETEIRAINVKLNFLAKEEQQIQEKIDRLETEKILHIKHMKRLYEEEHARFTKNNDYPLIGSRYQILSLLGKGGFSEVYKAFDLQEFKEVACKIHQLNSNWSDHAKQNYIRHAIRENGVHKELNHQNIVKLYDSVEIDKTSFCTVLELCDGPDLSYYIKKYKSFPEKEAKLLISQIISAIKYLNNHKNKIIHYDLKPQNILFHQNDLKISDFGLCKVLEDDNSKLQLTSQGVGTYWYLPPECFHMGDQPPSISSKVDIWSIGVIFFEMLFGQKPFGQGLSQEKILKEQIIIKSQTVTFPQKPIISNECKEFIRGCLAYNQVDRLDVHQAFNHPYFLKKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPH0</accession>
    <name>A0A8S1JPH0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Anoctamin transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0060306</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPH0"/>
    <dbReference type="OMA" id="IINCLTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF73">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="595"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="674"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="744"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="774"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="895"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="928"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="327"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="7">
        <location>
            <begin position="551"/>
            <end position="1012"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8044224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1036" mass="121495" checksum="91E80D7F39C59DCA" modified="2022-10-12" version="1">MHFFVHEEINLLEAAQDGKLDFIKKQLLSLTSKQLDPLTSKHLQDACKKSDYYGRNALHYAAYRGHYDVVEYFLDLHCLDLNSKDNQGNSALMLVCVRGYNQDIDESIIKSRDKQLIAETKYKIAKLLLQRGATLGEYLKDYINNPLHWACFYGDFKLTQLLLLKNPNLMLKKNDRKQFPIDIALMKGKDQETQKKVVKYLIVKFLAHFLKSEQHIQQLKSLDENMEESEFDLNQEEIHQNTVDRRKYETVGLNYLFWASVLGDLELVKPLLKQQFSPFAPSYKGRNAVHAAAYHGHDKLIELFFESENQNKKFNVKKFVNIMTIEKPQSALHIAVEQGHEKVVRYLIKIGADHHLYNFRNQTAFASSRSIAIKELRQNLLKTEKNLIKSGYKYVLVGTNASENLVKQQLDNIKAKVKVGKFKAVPIRSYDESCFYYIVKVSSELKNLVADYEKMMIYNFKEGIICQFDRNKAETYENFHHYHDQQIILSLLYDEFDLDQFLHDKLLLDHFPLHDDEEKVLIYNQWKREKWELLKEPLSLKQNKCRTPSAIKAYFGAEVGFFFVFLSFFTTWLFIPAVPGIMLGVYVYATNEINSLFVPIYTIALAVWATIFFEFWKRKQSEMMFQFDMHVETEEKQTIPSFKGQFWIEDVTHKIEIKYSTRDRWKYYKSVTPLVLLAIILIAGEQIGYYYIIQLRERTTQFTILCSIGLGISIKITNEIFNYFARLSLEYENHQYQNELEDVYIIKVFSFAFLNSFGRLFYKSVIDPDADELNIMSITFTIVWAIMHYLRFTIIPLIQYSIKKYFLNKEFELFLEGKWRKMYTSQMASILDNETQNQTNKSFDRKSMGYKKFLRSVEQNKIMIPTPNHVDQFTYFILQFCMVTMFSASSQLIPIAILLFNNINLDGLLYGFITFVKRPRAEAKKSIGLWNSVLLIVGYIGTIINCLTIYYANQDQLNNLIGKVNETDESSQALRNFLLLIAAEHIVIGIKFIIETVIPDEPGWVTKVLKRQEYLLEQMMKNIEIEGSQSDKLKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPM7</accession>
    <name>A0A8S1JPM7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-haem dioxygenase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPM7"/>
    <dbReference type="OMA" id="LYIHSRT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000158">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48420">
        <property type="entry name" value="NON-HAEM DIOXYGENASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48420:SF1">
        <property type="entry name" value="NON-HAEM DIOXYGENASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="41003" checksum="6405B228FC23016E" modified="2022-10-12" version="1">MLKKIFVRAYFQTVKAVRFTYDEIQNCSSNLNAKIEEAYGSHGLGLAIVSGIPNYSKMRLQLLPLAQKLAVQPQEYLKTLERPESFYSKGWSCGVEQFKGKFDKSKGSFYNNPNYDKCQELGKEYEHLMKKGSLIRIENVWPNRIPELEGAFKNLGRLMVDTGALLSYHIDKYIFSKCNTYEMGKLYRFVRTGDSHVGRLLHYYDGPNTDEWCGWHNDHSALTALTCPIYMHNDKIVDYTDQEGGLLAKNRYAEIMKVGMDPDCLAFQIGETAQIVSGGIVEATPHCVVKSDETVRRQLNRNTFAVFMGPLFNEVLNVPKGIDKDNALNKPAYNIPALLGRWDENMTFLEYSANSLKAYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRF7</accession>
    <name>A0A8S1JRF7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRF7"/>
    <dbReference type="OMA" id="FIQQTSH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="446"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="486" mass="59926" checksum="161AE6EA0D649093" modified="2022-10-12" version="1">MAYYREEPLQKGDYHNRIPNHNNHKTIKPVYNDDHKNNENYLQNPKNQQSYMDIEVQDRNNKQQYQGNNNDDDPFPDNPYYNKAALDKVCSKSIITFKDQPNRFQDDLKNNELQRDNYNQINQQQQNEPISKYYNNDQRDRYQEVKNYYYNQNHQQKSILSSKLYSRDHLDQYQESQKQTQNKQLVNQQQIYQYANEDLQENPSFGQIQDYQQSQQRQQQQQQDYKQSQQRQQQQDYQQSQQRQQQQYQYQPIQQQFSYSNQSSQEFKNQNTQQYISNSTRQQTINMNEHPSQREVQQQRQCVTYQTQSPQRFHQDLSIQKQQQYQQRFQYLEELKSRLPKDDMFYSQIDKRMPQTEQYQRQEQNERIARINPIAAVSRIQKPNQITQDNQNYSKDQYQQYQQQKREDYLIQSNRNNSNQYLNLYKSNKHYDNYQNVQNQQINNYNQKTRTPNHYREDSYERERQQFIQQTSHIKTNSYTNNNKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSE7</accession>
    <name>A0A8S1JSE7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080546</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSE7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="148" mass="17720" checksum="264867873879AB90" modified="2022-10-12" version="1">MIYSQQSKIYKILFIFKILQQINNVLNSKIQKQPNSIENNKLFFNIQKYLSSSTISIIHFKIRMIIFIISKLLKNFTLNLKKSHSNNSIIQEINNQNNLMLNYHQRLQNYGKNLIIWINFNQQIRNFCLVVKPVMSQIYKIPSRQDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSV2</accession>
    <name>A0A8S1JSV2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSV2"/>
    <dbReference type="OMA" id="HQQEFLI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="291" mass="34689" checksum="E950444D322D68B6" modified="2022-10-12" version="1">MDQEIVDQLTLDSIPYYEEQLYGALQVIIDLQQKVEQHEMILDDENSNENQTEDVEGDFQEIISTIQQQQLKELQDIDEISQKQQEDFDQLFKQLHELEQLTNQYQQENNQDEQYIKITQQIIFERQQQLKELSQQFMLQDIHNKENQRIIQNLQQKMKLDAVSIPQEGSKEEKININQFVQHQRQESEEDVDYIQYLQLTDYKQKLEEELEQIVNQPKVHKDKIDASTQTHQQEFLIKSKNNEDFAYSSSCVQPTFSVENTPKTSQILLHSLLKEEQPKNRECCTSCIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTK1</accession>
    <name>A0A8S1JTK1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0100154</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTK1"/>
    <dbReference type="OMA" id="MGKMGQH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000726">
        <property type="entry name" value="Calcium-dependent protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000634">
        <property type="entry name" value="Calcium-dependent protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000382">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="34"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="352"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="442"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="478"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8046042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="533" mass="62083" checksum="DB1E5C861EC4D4BB" modified="2022-10-12" version="1">MGCLQPKKLNRIVAMQTTNKDLVFTSTADIHKLYSFGKVLGIGAFGKVLVAKMRNNNSKQYAIKMIDKRKVRGREAMLANEIYVLQKLDHPNIIKFYEVYQNELYFYIIMDYCEGGELVERIQKSQKNLSEGQVQNIIFKICSAIMYIHEQGIIHRDIKPENILFSTKDPNAEPKLIDFGLAIKFDSSNLKQLKAAVGTPLYLAPEVIEGKYNEKCDVWSLGILLFHLLCGYPPFYGKNRTDLYENIQYQNLIFDRRHWNNVSDEAKDLIKKMLNKNHNIRPSAKDCLRHSWFRRKFQNPVRLQRGKSTIISMKSPQSDDQRSIYQMLKTFRIGAKFKKEVMKVLVNQMNEKELGRLKQVFQKIDVDNSGTITVQELKEALMEEGSHISHEEIEQLIQLVGFEIVEEEKDDNDCASTTKSSKPLVIKYSDFLAACIDERRVLTREKLWSVFKYFDTQDANFIMKEDIKEALARHGRQLSDEKIDEMIYEIDPNHDNKISFEEFCQMMGVAGVEQTMDFKDEVKQHLIFNQSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVZ0</accession>
    <name>A0A8S1JVZ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0080216</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVZ0"/>
    <dbReference type="OMA" id="MWLTYMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06174">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF9">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8044650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="563" mass="64639" checksum="C4225D728630279E" modified="2022-10-12" version="1">MENRNLSIYLLNFVTNALINFDHGIIPACTTDMKLDLGIDDIGVQWVHQFMEDLQQDHLQAQAYLTNIKLNIQLQSVYYVAYQVYLYLFQPKIQFSFLYRDFQQDSFKYAILIYLVFLVVFFPVWIDLFGENHKTMWLTYMQLGVALGTVSGYLVTSLSLHFSQNWVYPYYLQIILLIPCFLFYSFCDETMLSTKEQDKKEIKKENFKTIFLQNLKTFMSNPVFVFSMLSLSSLFFVVTGVQFWITDYLRNVLLMPHEVVFKSFLIISLSAPLTGVYIGGLILHNFGGYDGQMAIKIAAFEALLASISGAFIPILNNGTFVLLFLWFLLFFGGSAVPAIMGIMITSIPREQRSSGNSLSHLFQELLGYLPAPVLYGYVQQQTGGSTSRWGMILLSIVGFFGLFYIVLAIFWKSKQTLQLVSSVNESELRDRVPQLNSIDKNDIIKKSEFAQIRMQTSVQSGKSIMIPVQQDQINNEIQNENQTSLIHRTHTFKTRDAYQRQASVSQALTNFSILLGRGSLDNDLFSIKNNQKQMQNQQKEIELQVAGGFYLVSEGRYVKFEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JW12</accession>
    <name>A0A8S1JW12_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0080236</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JW12"/>
    <dbReference type="OMA" id="NAYESCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018256">
        <property type="entry name" value="Ribosomal_eL13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01104">
        <property type="entry name" value="RIBOSOMAL_L13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8044670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="208" mass="24214" checksum="4AEA6944BB6A3767" modified="2022-10-12" version="1">MKHNQQLPNAHLRKHWTRFVKTFYNQPAAKRRRQQRRQANALRASPRPVELLRPVVRGQTIKYNGIQKLGRGFSLIELKEAGLNAQFARTVGIAVDHRRRNNSQEELAANVKRIKAYLSKLVLFPRVAGKPKNGVVKDSANNVVEQPFTQNTNPEVITFQRTPKREKATVISKELRAKNVYRRLRQEWYNAKFVGVKEKRKQAKETKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWF2</accession>
    <name>A0A8S1JWF2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWF2"/>
    <dbReference type="OMA" id="QMPHSAK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="135" mass="15556" checksum="E730D993A2971A11" modified="2022-10-12" version="1">MSEFQQQMPHSSKLDQESATNAKNHLLLVNNVTELSNELLQSYKHEIDQLKQELHLMKQRITNNNEEIKNTTQPTLDAMLRDLRQAINTQKDENSKLQSQITELKKEKSQIQQLIIAATQKLAELENQVGNYTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1X5</accession>
    <name>A0A8S1K1X5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vacuolar protein sorting-associated protein 51 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0130101</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the Golgi-associated retrograde protein (GARP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the VPS51 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1X5"/>
    <dbReference type="OMA" id="EHWINIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990745">
        <property type="term" value="C:EARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007041">
        <property type="term" value="P:lysosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014812">
        <property type="entry name" value="Vps51"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954:SF4">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="2" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU368010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8048839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="787" mass="92815" checksum="D865DBD61A8F8BFF" modified="2022-10-12" version="1">MDQVDIDSNKFDCQKYVGEFLKNHSVQDLIQRHNQLQQEIKEYDQDIQSLVFENYSKFISSIDTVKKMKTDITQIDKKVETLAQSMSRIAQLSRRIDSQLSIKREEIIKLDTVNKDLSRLNSVCNFPHVIQKELDQYKISKGKNYDEYFNETAQYYKQCYSTLDQYKNEPLIQPIYKETNQKLDNTRQILWSSLIEFKQQNPLLQLYSESKVSISTQEARSAVKKLIQIMDDEEAVLRHYQKIIKERYLTVAKELIDKAAITKSSEVVNLMDYLEDKRYDYTVEELEEYKQMKEKEIVNSDSAIQGSILWLINQLCQIIFKELKVEVSFLLIEIRGTLRDNTLEQLSTQCIKEIIHGVFQGVTDKMSQKKINNVQVNEVFVKLQTELQSLFENNSIRQQLRTEIIDKFTENVENICRGQVFTSFSHLRVQFLELLVFAKNDLQRLQSTDEIKREIMTLITVIKQKLIALTTIRLLDLKAFVFSQQQSYLREQETYLNLIQASLIEFVKFIGKIFAFRSISISQDQLNQLFNDNYIIQQVLPLLNTNDSTYYYLFSNQFLQLYSKTLSNKLFELVSQVFQGYKIRQPTDQELNKLKSYINGSTQKNLMLFAQAQSEHWINIMFQYLNEENIVYQQQITQKMLLNEVKNFIEALGLLFPDTKRGLVKKVSIIQQSTFNIQMEIVMVRKCKILETNINDRNSLLITVVKYVLKVLLQKVRRTYFSEDLFKQFQVDLYFVMQLFYDMVSVDEEGLIAGFYMEILDCAIKNSRNDSKIDPKILENLSLQVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2A8</accession>
    <name>A0A8S1K2A8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0130415</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2A8"/>
    <dbReference type="OMA" id="RWIDSHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="299"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8049339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="35959" checksum="CEE2FAC0D238DE20" modified="2022-10-12" version="1">MGNRQAQEEEIDDNRFNAIQRGLLKIQSGFQVFRNQQDEKKIKKLDEIDRPKNATLCPEFYDMIAEEYRIQFDKKTVKKKFKRYPPFRLENGDIYEGCWIHGKKHGLGILLLQNGTIIQGEWQNDALLDGRIIYPNGDIQVGNQYTYANGIIFIGDDDYGIEKHPDGSIYTGEFHNGHKHGRGQFIYSDGSVYSGDFVEDLYSGYGELKYYNKIVYEGQWFNGQMHGLGKLKWPDGREYEGEFKYGLKNGPGKMKYSDKSEFHGIFENGLKVGKGRWIDSHQQLQEREIENGHMLDPQLKPPPPLQQQND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4Q1</accession>
    <name>A0A8S1K4Q1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4Q1"/>
    <dbReference type="OMA" id="MTFRYSS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="24629" checksum="D21A8EB7FF9EDDCE" modified="2022-10-12" version="1">MTFRYSSELTLNIYEKPPQNPEWLQKQENIDLILNKIKQHNMLKRIHYTNNEQLQENDPLLLRSHRLNKKLEEFDHYHEEQLETYRKILQSDREQITNAMSQRRRMGSFVKHVRSISTTVLKPLIQVNNIVNLQGNEKKSDNHIIMRQKIKSIQENRKTIDNCSKRLQQLFKEDSSNQVSRVQSHLEQRQKKKKISIDLIRNLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6X0</accession>
    <name>A0A8S1K6X0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0170350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8051019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6X0"/>
    <dbReference type="OMA" id="NNEYMML"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="142"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8051019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="496" mass="59037" checksum="DB2CAD84183FD632" modified="2022-10-12" version="1">MNNLKSKNSAPLDKPQKYPNSEILSIPMHQMRIDINNNNKNRLDSQQSSKNLEILRQSHQSSTSNLRLDNPLNKSSENLIKKTPTRTKVCTSIDKIPYPLQNNFSLSPHSAFMKPQIKQEDDQQNIINFCHQKSNLIIDHRKEQIILQLQQAQQLLVLKEQENQKLQEINKRLYEENQKLYNQGDDMLKDIETMRQNFDQQNNEYMMLKQQNQQLIEDVKLIQNERNQTQSHSDQILNLKNYEIQQLQNQLREYQQLLQNKDQTISNLKQKLDIVEQQEICRQNLKQSQKSSEKSYLNNNNQQIENTQKIQNQDLNSKGSQMQSDIQYYQTQLEQISSEKDICEQKLVELQTILNQFVLNEQQQNITSNSNQNVIYQKQYNKLKQIVQIQKKQIESQQKTIMNQHYEIQNLQNLLYSIEQDCSSQKNSNIFYEKFSIQQPNICDIRQKESNEREPIDKIGFQRILFKQQQDSNVVVEDTFRNMQKEQELFTSTYDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K716</accession>
    <name>A0A8S1K716_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100237</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K716"/>
    <dbReference type="OMA" id="NEQEPKD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="36118" checksum="D8A1F3716CC027AE" modified="2022-10-12" version="1">MRRPNEVEFSKTALMANWYEDRLAPQQLQRDPNATKSLRGVEEGISIPGDNGCLLPLPRVNRNPPYITKEIIVPDDGYREFRTEFQTKFDAKNIQNMQLGDCRSLIKTNGDKVNFPECNSTVAQTAGAKSIKQLDQTNMHQILVDRAVHQNYTDFGSTFRNHPEQHNKFYAITTYQQSFQQPENNVAEILTDKKKKPQASGCRERDPFDQGLKMTSVITGEKYRDSIDPKENTQVQRQWIHCGDAGLKTAQNNLRQSSMENYKKNASQVHPYDIATSLPLADGVYTLHPKYTDPGQFRHIRSDVTRIMNKPITRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7P0</accession>
    <name>A0A8S1K7P0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0100413</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7P0"/>
    <dbReference type="OMA" id="EYANQYP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08291">
        <property type="entry name" value="ETR_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF18">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="19"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8046518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="335" mass="36451" checksum="0DE322F4EA171C23" modified="2022-10-12" version="1">MEQFTIPKTFKAAQLVEYGKDLQIVEIETPQLKDGQLLIKVEAAPVNPSDLLLIAGHYPAGKVLPAVPGIEGSGIVVQLGPNVDNVKLGTKVAFTASSNYGSYAQYAVTTSQSVIPLDDDISFELGASSIVNPVTVLLMLVETQELGAKAIVHTAAGSALGRMLVRYFQDSGIDVINVVRRQEQADLLKQEGAKYVLNQTSETFFQDLNALATQLNATVFFDAIGGSLTGQILTQLPNKSTALVYGLLSGQPISDVTANDLLFKSKTVKGFWLATSVHKYNPFADVNAQKKLNELLKTTLKTEYANQYPLEKIVEGIEYYKVNQTSRKILIRPNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9J4</accession>
    <name>A0A8S1K9J4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0170008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9J4"/>
    <dbReference type="OMA" id="EYELYIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="338" mass="40464" checksum="0D873BEC8265C2BB" modified="2022-10-12" version="1">MKKIIKYFKDFQSQTEFEIVLRRILESSKLHLVNELKYQVLQEGFNGQIQELHVQQSFKILYQHFKSCRNDIASLKILIIIHRLLLSCRDFGDLVDLDQVLIYYRQPIKQLPILQQLYQNLIFQYRDYLKGITERNQYFLLCSCKINDFYQMDFKNQLASQIVIVFILKKLKLFIPYLENILVKKSNSLIQNLIILILNDAKNMYEFIAQFVLQNQCFKLLLNQIVSWISLQIILQICQLNYWIIKLFQLGNLAEISDKLEFPQIFEINSAQNKEYELYIQLIRNNYEIGSPDFSPKFQDLNQFKKQEEFQIILLKPQQLNTVLGQSERPLEYIESNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB41</accession>
    <name>A0A8S1KB41_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0140344</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB41"/>
    <dbReference type="OMA" id="LICRGCA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="224"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8050146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="298" mass="35485" checksum="2451AF66FB249454" modified="2022-10-12" version="1">MIKFDEVQKRALICRGCAGRTLGYDKYCCNENKFINILKQHYIDGTNIAQLREMLDLTQGCENFCEVHGYLYEFVLTKINQNNDKQLIYEDQILCCKKCIKEEKNLKDYDIQLLNDEYFTYQQEDQNIEDQQKQASYEREFQKQKQGLEDQNNQDQQKQASYKREFLIRKQFVEVAMNDAFKNMRIALKEPSKLEIKIGEAEERKAEIKRMNLQQGQQQVYNQNQEDKEKVENLKLLKNAIEQRIRAISNLGDGYHDYLHDEFLEGESVRQKIIGDDSQTKGYSINECYKMFDNIQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEI6</accession>
    <name>A0A8S1KEI6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0190051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEI6"/>
    <dbReference type="OMA" id="CTQWINC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044661">
        <property type="entry name" value="MED15a/b/c-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="340"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="427"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="526" mass="63318" checksum="93C54856B38AB834" modified="2022-10-12" version="1">MTQFHKGFTWTQCTSTLKTKNTFINLRQTVTPNQGPVLLKIDTERELKYTNDLGWNWHKSWNIPNISVKISLQNEECTQWINCQVQLMAVKLLQNDLFEEVGLEGITQYDIIDGKAFFSGVKFNSTTYNHQGHRFQLLILLKEKENIILGLQSPPVFVDSRKSARDEHRQIQYIQPFEPRYLERNFCKKEKHFNDVVDAPIENNENGLHNYLTAPNIRNKIKHPLFMALKFSRCLNIYYQNNLETDNYLIEFQKQLMSKYQQSQYIIVFQSNNNRIRKKIEESLTQLFGQSMISVVERKYLDETQFKKLEFSSDDYSLIYTQLQELMNQYSQDQYNQSVKQQQEQQQSDMNIEQQQQQQQEQQQQQEQQQQQQQQQQQQQQQQQQQQQQQQQQPIQTEQLLQQQPPKILTHSERKSAFTKYLDKLPTKNFEEEKQEQQQRMEKLNNIREMENYDKVQKIVKVEEELIQNRAQQQQQQQQYSQQQYQQYAPINHPFSILLQQKLQEQILQYYFIQQQLMLLQKPPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KII0</accession>
    <name>A0A8S1KII0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0220064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KII0"/>
    <dbReference type="OMA" id="KIAYQTQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="329" mass="38352" checksum="F0E1C00A802731AB" modified="2022-10-12" version="1">MFQNSHTNNQFSFHSTIKFKKLPKLEPLFKQINQVVPQNTQRGRSFKYYLFNVTGGQCKFRRKTCTCSECGGTNTFVEKQNALQPITYIRAHHVKKRKRKMHRNKGGGRHLSAMINRVPLKIEDITNLIPTRKERKAKTLLQNQFIEQTLLKCKRLLTKDDSTIGYEYFFANELKIAYQTQEFMTNSTFEKTPTQIKQQNGNLTGSQRTIDESQVTIAQNLNINQSQVLNIQLPKINYKIKQKPSLYSPRIYQDILESHNLKPNKTVDKYISQSTHCSPVNIKLPLISQKKVIQTKRKKSEHVYLKGSPLKLLHELKSQILLQKKMSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKZ6</accession>
    <name>A0A8S1KKZ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0180252</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKZ6"/>
    <dbReference type="OMA" id="RVEYDNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015165">
        <property type="term" value="F:pyrimidine nucleotide-sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007271">
        <property type="entry name" value="Nuc_sug_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13146">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13146:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04142">
        <property type="entry name" value="Nuc_sug_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8051634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="403" mass="45006" checksum="D146D2BBEED0B650" modified="2022-10-12" version="1">MGEKSASFITLLITAMILSGAANTIVYKLQNTSKVSVDGWTATNFNHPFMQAVTMFLGESLCILLFLNIKKKPDYKQGCIEAAAKGLKTKVNYGWVAIPAMCDLTASTLAYISLNYIPPSIYQMLRGGAIISTAIMSTCFLKRHIKRYQWFGCFFVLVGITLVGMSSFLFPPKKDNNDDSDTKSEIGAAYFISVGLLLLSVVMNGLQFVSEEKLFDVYYLHPFEIVGIEGLWGFSVYVVLAIALTFIQCPTSMEGSCIQKRMTDMFYFERADFYFLQIFASGLLLFWVILGVFTIATFNICGVSVTKYVSSLARSLVDVSRTLIIWAVSLAITWIDPEAKWENTRWEAIVLELIGFFVLVTGNLIYNGTIKLKFLDEGSQVDPLNDAAQQFLDSKNIQSLQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLB8</accession>
    <name>A0A8S1KLB8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0240060</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLB8"/>
    <dbReference type="OMA" id="DSCITIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46726">
        <property type="entry name" value="TWO PORE CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46726:SF1">
        <property type="entry name" value="TWO-PORE CALCIUM CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="493"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="564"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="627"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="667"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="359"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="692"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8055958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1189" mass="140206" checksum="164E426A4B3C31B1" modified="2022-10-12" version="1">MKKTHKRQSTIYRQSIAMSEFSQKLLEKNDVLAQEIQKIVKKLSPQCRIEKAERHIYDAYYLRAIRNDIDLVNKTYLKQLWYLENNQYWLNGLFLLSIIYQLLTFFEPSFVDQDDQDHPNLFIIELIILLFMGIDSCITIVLLLTKKEGRFTFNPKKQFKLIAYYFCLVDFIIHSYEPTLFRVSKICRTLLMPMYSKDLRRNLKGIFKAGKDLFLLIILYLIIISIFSFIGINLIGKLDNVDLRTQDYGDFFKLFSMLFMVATLDFYPDILIPPMLQGIYYCLFFITYLLLFIFLFAPIPLAVVYEGFRKHRMEIAISDIIKQKTAMMASFISLDFQDQGFLTRDQFRTFILHFYKNSITESQINQLFNQIDQDFNDKVQFDEFHKFLHLLQDASVFSLPETKPLHCWEQFRNYLISKGLLTFVEGNIFGISMLIITISNCVLIVVAFFIEDQDILDIFNLLDTIFLILYSLECSIKIIALGIKPYFEEGWNIFDITLVILQIIFDYILFNIVSGNIVQSIKANRLLRLAKIQKVFRLFRAFRSLKIINILFEGLQFLDVVRTLLYKIIICVPLILRLMLPVQMVFFIYSCVGMYLYGKIQRNEDNPYANSQCDINNFEYSWGSCKYADFSTFAGSYLMMLQMFIAAEWNQIVFELTYDTDDMVSAMLFVGSFEFFSIFLLALIGGLVWEVFTVVSQSLKQEEDRLSQLENNLDQQQENNNNNQAATHDKKKKLILNDDNPDEIRFHRKFRSDKISKTHTKLNNIQPGFEDDIVNDERPELNISQPNSRNYFLYHGGSGRRVVPFQSSKFNIQRNDVIDHFLKNFITADMWLQKKQIEGLDINKVAIDYMIHLRSEIKKDEMFQKKNFNISNHHLLIQNAVLRDASEVYIKQQEDKFFKTSFGQKFERIQELKFQSKFKIESKIIFSLMGILKFPNPNLKYYFQILNLIENYFTYQLLSDYSFFKLIHQINNKWYTISIEEGQIYFFKVGNGPWDYTEQLFSESNIRICENLSSLLESGNQECLRAIEQFSISVKSIAKNFEISLPSIDINNSCILYQIKNEKLIVNDIQSLKIKKSNVTSDDQSPNNQQYVYLDSQNQLNGFLKLNQSPKIETSNLDEQKNVNQTILFVIARTTAQEQRIKYQNITMLIQFIQDLGSVIQNYSSTFFKQINNLYELRYQDKIKSMRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQ92</accession>
    <name>A0A8S1KQ92_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0260140</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQ92"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035874724" evidence="3">
        <location>
            <begin position="19"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="363"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8057630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="426" mass="48340" checksum="4D50570A898C8111" modified="2022-10-12" version="1">MKVFAIMALLLITAYSQSNEIDVVLKMLADLKNGTIKQLQELESDWEATKYQKQDIVNDLQRSASTQRGECNRRDQEWANKERDIRITLSYINWLEKRIKENNERLQRLDVNRCESNANFINDIKNSKKTLNLIAFLRRAVKNAKETDLPTLLQSAQFIQLKSFLADDGEELELTSEDNEPPHVYDEVTETPEETSVTEDVEQELETSAAPEEGVEAADHEIEEAQQVDQTEQPVPEHPLEAEEKATNEQLAKEAAAFNKIIEKGQKAPAQSGSGKDYSQFTVAQQELLNFLDVLEDQVRGSFGNKQDNQVNSAMGYSDFKGLITKENETFRGHLAAEDVNLEKLQNQLITILQATAACKDRLKKIQNSIDLANEDLANAEAHFKSVSETLQEEQNAFDDVYRIYSSQVGSQSTQYKRDVQSKITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KS15</accession>
    <name>A0A8S1KS15_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KS15"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="211" mass="24900" checksum="94C2AAB8A49DC371" modified="2022-10-12" version="1">MIFENNLIEFSHDLELLINDMEMQSKQLIINQILNCYLILKLYQIIEYKECLFFRSSNDTNSQNYLRILIISLIAFNIFHIQPSLVIILKNSLNQQLPNTSCIFISKIIKEESYDLVGKQAEQTILQFDQKEDDYQIQKNMFMHQIQKSYQKGFGLVVKGIISQEKILFCLRNTMIQVLTPLFLIPIIQKNNFFILRLTVNHLKNGEVFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTJ1</accession>
    <name>A0A8S1KTJ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Chromatin modification-related protein MEAF6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0250329</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EAF6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTJ1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015418">
        <property type="entry name" value="Eaf6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13476">
        <property type="entry name" value="CHROMATIN MODIFICATION-RELATED PROTEIN MEAF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09340">
        <property type="entry name" value="NuA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8057215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="18006" checksum="F1810BA8B91AF5F9" modified="2022-10-12" version="1">MDKKYQDLVDKKLILENELKNLEKNIFDEETRYLEETGHIGNVIKGWEGFLSMKNSKLGGNQQKKGKINPNDRIFSQSSKTSPFVQEISQPVQITGTQKQVLNEGVGEEKKEYHFRRTKKNNKYVRFQKGEGGYHSPMTSSDEYNEKKGKVIKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUU7</accession>
    <name>A0A8S1KUU7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUU7"/>
    <dbReference type="OMA" id="FKQWINP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="829" mass="98503" checksum="39328FAAFCEEBC6D" modified="2022-10-12" version="1">MDNQYQIAKNLLQSILIIKQQDSNLDSAYSSILLALQSQLPLKQEHYTSILIEITKKLTKQIEQYDEYYTSQMLKILQNIPKQQYIDQLQCTISILSTLINLLAKVNQTFIPEIFQLISLLIDNSKALQIAYPQLFIKTLTCLLKYSRGVIQENGLLLFEKILSYIVCNTQSQYQQLNFNVISLKYEENQNYLVQQKVNEELQNFCLFITHLPTPKLNKCYSQLIFKIFQYSNSGQYFYISTQISLINLYFRFNEGQNIQEFTNLIIQMNSQQQLRIYNELEEDLEFELKRFHSLQNEVDTSQTIAIIRKIKWLIYIIIEFQKKQQNQLQYVQISEKIIKYLVQGLQYKWNQIVVSVDQQVTTLNQNLNISIKNNINKLNLELMPILNNEQSILNEIISLLNSLNENEIINKYLENKLEKQLNKLIQTIIVDDSQIIKIQITQILYTSSILFILNNISSTQKHDSYYFKNLIKQFSMLIFNQNAPYNTIFQSQLIIYSNLIYQRIDEKDDQFIEVLYLDYLMCQNSQSTVLITEISQNYIKQFNIYDHIASILYRNIQKIQESNLQVDQGNSLQTILHIYSKFDGYNQGLDIIYQVLKTIYQIYDKYYLKSDILFTQKYLQFLILFFKQWINPSLNNNIVTILRQIALSLTQFFHHSQVHFQAFQCFYYCIPILAKQELQECNSKQVFGMNDDPNVKVVDTLGTIFFEQRKSFEYILTQDNQISKLYIWMIIKELLIYDSCQGFFQLKSIQQFLKQYLVFEQEENSNSLKIYEQILQFLILYCKKHEQNPNLIDLLKQIEFFIKKCTKLDASQLKELCNHLNQKLSLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVW2</accession>
    <name>A0A8S1KVW2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8058025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVW2"/>
    <dbReference type="OMA" id="IHCHINR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="1">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="147" mass="17105" checksum="F55F7E032A66825D" modified="2022-10-12" version="1">MYLGSRDGLKAEYFWNKVNNKDNLLMVFKSKSGSIFGAYSPCKWDYSGSANKQDDTLSSFIFSQTHDQIYNLKENNKNQAIHCHINRGPSYGNYNDININGDFTDGYSELGCDYQFDRNNNKNYKTHLYGQEKPEIQECVIYQIQFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWH6</accession>
    <name>A0A8S1KWH6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0270114</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWH6"/>
    <dbReference type="OMA" id="KFAKKEP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="557"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="87"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="433"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="94"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8058235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="804" mass="94975" checksum="E7B286EDDDBF970D" modified="2022-10-12" version="1">MWEKKLQISCSLFTEVQLECVIRKSLCKKSFHFDCVDNPIISKTEENGHRPPQLLIFCSYHDSIENVVTDLDEAVNSLYNSYIIQKEQQEEPHSHHRSKHKKKHKKKHNHKRSRSYRKKSRHSSKNWKFSDGEDSESKKSISYIIQPPQPNKSLLIIQNSIITNDLIVQALSKKQQSKPQIEIESQVPLTLNYQIQQQNHQQQQQIKPLLLPQYEQQQQQQQQQQQQQQQSQIQIENQKNTIIKQQIYPQITKISLEDPEFPRQERETKRDQSIAKWWDKIDDVFFNGEEKMPQTNLEEFDNLFKWHEQTLDQDLLDYSDQSNFEPIIQLRYLYRDSMNVVFRTSQSSYQSLQILDFIDIHQKVSLYQEIYKFLKMKYKYKFAKKEPVGKSLVFQESCELSQEDFDQCIKLEEKIKSQYQLLELQLDIENETVEQLDIVYHALKEDLNEIVQENNKMRQNINNQIINTKDHATLETMKKQQQKIMDLLKWSQIVKAFINGYKDKQKDVLNTFYPCLNLNSQSSQKAELKKKKIHSKENSKPLDTDCKICFDYHYTDFNPVIYCGRCSTSFHKICYCFIGNLDEQDVLCDACQFEQNKLSITKRNQAKCRICKKLGLPQKQIDNQFYHVSCLLLTNLVIIKKGVYKLRNKSDIKQFCKDNETSTPQCAICGDVKGFRFTCFGNETIPCRHAFHPLCAYLHGLTIDIESEDIEQCFTEQRFGQLNVQIKCVLHCGKDLQDLLQQTYYRRFALNYEQAAQCGGQDIFLEGFKTTKGYKYLYSKQPISKNDSLLSQIYNQNGTTTTMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L156</accession>
    <name>A0A8S1L156_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0320067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L156"/>
    <dbReference type="OMA" id="MFMINSA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="603"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="652"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="681"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="723"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="823"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="863"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="935"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="957"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="987"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1035"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1040" mass="123951" checksum="B1C1A679BA2B419C" modified="2022-10-12" version="1">MQNHGIEFLFIKGNTKCADSTQLQSVKQYHPKLNSLRMKGFQQFFDLMIKQQQDQQQMKTCSQCTPQLLPTVLSYPPRIEIHNRKSSETKLLMKKNLLETLPDSNSQEKCKKELRLIPQKQFQVKKTITNKNEINKENQEIIELPIMIAFTNNINVVPPERTFYKAFITKGNNGTLIRQSLKLRPWWILIDQPNEDLNLYWTQLRKQQEYDSVKQFQLENVQFTKQQKKLINDKIKYNIEESEFQLFNQSSILRVHNHLECNFQVCNKKALFYNMKNYYLSINEDPFQFIPLTYHVKTGLNDPAFLEFEEFAKTNNINVWIIKPGESSNRGNGIQVANSITKVKQIISQQNLHLNNSKKTFIIQKYLEKPLLYNKRKFDIRCYMLMTCVNNTFKAYWYQEGYIRTSCKEFNLDDVEDKFTHLTNDAVQKKNQNYGKFETGNKISYNEFQKYLNDQHNYNFQIILDQLKKLSIDIVKATYQHLNPNKLFYSFELFGLDFMIDSDMKPWLIEVNTNPCLETCCPLLARLITHLIENTIKIAIDPMYPPPLSKKKHLQESKNLMELVFSSTLINERISQPMIKEDDDEHSDNKDGQRYNYCNYQEYVVLVIWCFYIAIATVYSIVYLSTSKFNGTMEFPLLGWTIDFLDPEWRVLYQYLQELLIYLLCYFFFRHLFRKQRMPFYIFSGIIFGGYLSRFGFAINLILMFANFIFVKLCKQSAIFLKIHWILHLIIIYLINIFNGLNDIWGDSGKEFNQAAPIPWQHIYIWNMLRMTSYNVEVQEINLVEYLAYLLYPPLYYSGPLIQFNDWQIQIRINLTVIKKHKYIINGLILLSLQILTQCYSVTNHKENEWLWDKQITTLSGVSFFVTSAIQIYFRFIFLWALQECWASYDDIDIQTNNPKHMFMINSAKSFEKYFCATYKNFLEKYVKDIFSQNINLNLLLIYSITILLVNSDFKTIWFYITLFLFHLFEDKIQFQNKLFNITIRSIQQCIILLEIIIMYTSDIDIIWIYVKQKNLYWIPLIFGTCLLTQIYLSLTKQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2N2</accession>
    <name>A0A8S1L2N2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">VLIG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0280116</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NIDNEIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030383">
        <property type="entry name" value="G_VLIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052986">
        <property type="entry name" value="VLIG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819:SF25">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_52, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819">
        <property type="entry name" value="GTP-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25683">
        <property type="entry name" value="URGCP_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51717">
        <property type="entry name" value="G_VLIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VLIG-type G" evidence="3">
        <location>
            <begin position="1138"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="2326"/>
            <end position="2503"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1011"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1467"/>
            <end position="1494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8058936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2510" mass="301037" checksum="41F7C9A2C62301F6" modified="2022-10-12" version="1">MNQSFPKISNNDVNKSLLTIFQKIKNKRYIRSAFILNDLKPIMEKLDNEEKQIIIEYMQKEFKDEEFLKHSGYVEQPNYKQLYQKARNEKDKQLIFQQLRNPQFNIDNEIHDLIYQNEKIVCRLEENIFDLLDLNEFRLGNIFITQIELLKILISFFFLPIMQDKPNLLEQIIGSQLIPQIYQFQYYLEIKQNANQISFDKLVQIIQHRQLQLISKEEFENTILKMKDYNFFLIINSLIENHQVFREILVQKFCQLLNCTKQQLKVESILSHLLNIENINEEIEELYFCIIFYFWMKYLIKYDIEQGFSDLQQINIMAEDTIETKEQAMQSIIQEQIKSNQDQILLHFVYIIFYFSKELSLINIQEYQRYKQERIKLQEKIGQNCSELSLQVWKDTTQLQYCQLNKIQGQGELIKFFWITQLNSEIFMIINNLLIEQQSLNLFEIAILLENKLKETKLNNTYLIISNILMLLPNNLSNVDKTQKILLIQLYLKYNTFNGQVQDLIKEIFFQKNNTNVKDNKFIQLCQRFYELDEQQYEIWLESVQYYQIFQITPKCLKHIKYHKQFPTNQKIAIEQFSFQVPEENNQQLKQAISNWVKCMSDNIIYDLFPFFIDILKRGESINLLVKLINEEIFNHQSKSEYFNSKSLFQIFYNNSNQELKFLLLKYRSNQYPIPLIYQTSFFNEIQRLENLYEINDKIYYLMQKSVNIINLSLSKIQSKIGKTELINNIFYKMQKFEIGDNCQINESTIDVMFDFEFNGTRKFMIADTHGYIPQEILIKILPLFQICIIQIDSEKELQENIDSIKSLQIYNSMNIYLLIRNSRKSKIPEQLEKELTQLKINYFQVSDLSQRGIDKQVQLTEIESVSKFLLNNICENQYSLKKSKQLFLNILQDFNKKSIEYSEIIIQNKTIFEQMKTELSKVIDLNDGFYNELAFPLRHSDYQNKILHQKIIEYSLQSVQEQTTIEKLREAIEENLMFKKFRVPSPLLNLFQKILQQDHVQYLWFLEKLRQFNEKNTQKLNEKIQNLIQKRSSSFTSNSQINFKTQSQDLNQQLHDAANQITQIENELKFKKIGIEIFWRELIQFECLGKQLSINPIDALCQIIRKGEPFELLDGDSQTIDKQFLQKLIQQLKQDENQKVLVLSVLGPQSSGKSTLWNKIFGCHFWTSVGRCTRGINIQLIKVRDKSIFGSLFDQILLLDTEGLQSPNQTDIEFDQKMSLFILAISDIILLTVKGDIHLQFHNLIEICIYQYGQLIQNIAGVKQIVWCFNQNNDVQKKGPFFEQIQQLAMRVEKRNYQSNPFDFEIQEQQNQQIDYANIIDIDTKHIWVLGFAQIQNIWDFQNWTQQSINETFSKTAYQNGILMIKNYMEKFELQNDINQLQTLSQFLLSVDETWKNIEKLPDYLEFSELLQYYQNKQMKIEYQNMLERRSRDFEFKNLIRAQINEAALKNKTSQEFFINLEDEKMIDINIRLNEIQEQISQELQEFKQSQKISKKIYLKYSEQLERQINNERLACKLIITEAIIQQKILYNKDQSLQNLGKSIRTLIDYSQNKQLDEQQILEQFEEKWDNIVKQFSQTLDIQFDKFQMLQYEIMKGQYNEFILRSTKEFEYINLLRNKINKTEPSQSKEEKKLIFKLYFEELKENQFRPIVQTDQKFINIYDKPIKQKIEEFEGSNYINFLNFYICQMQIEFVRKENLEDYLQNQIIKDFREFQIENQQNQYNLNFFSYFFDQFQLKIEIKLIGELLSENQDDNFEQLKNYFQSIQIPDQLEKQSNLNNFPKKNYKNLQISKLKEKYICSEVKAVLKEMHKNFYIISQNLDYEYNVNDYNQYRSYIDQIEYKSKNQRLQILNEFSKQLTSLMEKEEVWDKIYGEAYNLVENEMNVQQDVNKTNNEQVSCFNMQKINIIIEKLNVNLIQNKFNTQISYFGVILNKLGERCIIYFCLLIIWRFISYFQWLSVNKYINELNQMKLNQKEKTIAYLQENKTVLSIISARDLFDTIKQNFESQFYINKDVYFLQQIEDENFNPVVILDQEFRNNNQEFIINYISDQKSQIEKYVDNKLAVLKTRLNNELQKELTQEYEELIKNIKSNAQLLQQFLQPNLNIEEYFENTELGKDLETKLFEIVVCFLKGEKFSDKKLINYKQRYIFYQNEIVQVQIPLNNLQNTKIQLLDVFVNKLIELLEQNLQVKLDLDRFNQQEALLKKRQQLIGCDVTCEFCNRKCDLQYQHQSKHKCQYGHFLIVNMNELEQQYSCDEYFLVSKGNNFQQFNQNWSLQKDENKNEKKQLKQDLIKIWQQYGEKICQKLQKKYKKILEQINPKKIHYIFILDSSGSMSEDWTNIKIGVRDFISKIKEDDQQNNSESKVSLIIFDNEEKILMNSQRAKKIENHVKMNFLGGGTNLSNPIKKAIKIINKDNFSELYLILFHTDGRAAYPQQELQNLLQLNQAKREKIQLIACTQNANNQNEVLNKMINFFNEHTKQANLFTFDSAEDLIQFWSVVINFKLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4N5</accession>
    <name>A0A8S1L4N5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tubby C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0300183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4N5"/>
    <dbReference type="OMA" id="NDYIVMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF7">
        <property type="entry name" value="PROTEIN KING TUBBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubby C-terminal" evidence="2">
        <location>
            <begin position="331"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8060533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="572" mass="67174" checksum="F4E65C20C502FD91" modified="2022-10-12" version="1">MFLNPQYIEDDTQEPVNPNPQSSQRQQPKGRSNITKFSAKTKEEPKQDNKPLSRIQQKEIEIQKKQSESQIQQNDEMVESLVQFDSDPEQQDIKNQTTPLIDSQNKQEEHKPQQYTKPQDTLEFQQLSVLQSNKQYKKKLGQNESTYGQDFELEFEKFSIIGNNHNQYYEPGQQLSQDDSLPQLQKQNNKIQFIESQSLKFKPQENNVDDDKIIINKQIIKEEEDITQDNNNYQKLQEIIVQDQNEEEDEDENDDGLLSQQQKEQEIRKELKQQYDKIQMDITQDTHEVKEQQDNKQDIQQVPQEQPKQTCPYPKPLLSIEFPNGKKHFLLNPAPKGGMIQCTIKRDRSGMSRFYPKYHLHISNGFMYLMSSKKRACNNTSNYIISMSREDLDKGNNFIGKVRSNFMGTEFILYDAGLNPEKTKDQSKIRQQLGIIQYESNLLGSKGPRKMVVLLPNLDEREQMYVFKPTNSKDGILKEYLNNNRDHIVTYVNRPPQWNSRHKAFVLNFYQRVDKPSVKNFQLIVQNKEDNILLQFGRVGDDLFNLDFQYPITPLQAFQIALTSFDYKIACE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5N3</accession>
    <name>A0A8S1L5N3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0290338</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8060066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5N3"/>
    <dbReference type="OMA" id="THEVYRT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="620" mass="71421" checksum="974F6C79542B804D" modified="2022-10-12" version="1">MKPISLDQSQTKIKILQQVITIPDVPCQRHAKEQLVSVCTQGQCREKGAICPLCQVNYHHMHLEDTWNLNYFVQQIHKQIESLQPDYKAINELLISFKLKISESIKSLILQLEIIDKQVNMILQKSKQKIDFDIDEIKTTLKNLGKTSQIDNFGKKIVKLTQYFGIDPKTHEVYRTKDYEDLQIQKMTDLIEENQQYFEKVKERITRLLTKTLDKFCNFEIPQMEMVVSKQFEPKQLRCQQVIHRAHQNWIRSVSMFRLSEREIQKVKETAQGQLSEFDQSLRYGQMAIVTTSDDMDIKVWLDSPDDHKFHLLSTITGAHQREFFGLRIHYCDINLITRLSNEISCNNLIFSAQHGLIKVWDWISKKCVNEIKTQSFFTYDFEIVKMKDLGICFAHGSKNSIQIVDFTSPFGMLMIQEINPDEQNKDISTFRKISQVKEKFDYLAVGFINGVIKVFKNDGKLLYKIQRQDTELAFCVEFLRIVQKKTQNTKGPTELLVIGSDSGQISAYDIQTGKSVWVNRNSHTSFVRGLTSIIVKDSSSTKTILLSQSEKEGSINILDGANGQLLHSMQDALDSGLRFRGLSSYQGVLDPITPDSSIIVAGTCKGTLHIFTFQEQKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L7R2</accession>
    <name>A0A8S1L7R2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="ORF">PPRIM_AZ9-3.1.T0320068</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="31">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L7R2"/>
    <dbReference type="OMA" id="AHPWIQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000632">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000557">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 27" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="29">
        <location>
            <begin position="49"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="30">
        <location>
            <begin position="346"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="30">
        <location>
            <begin position="383"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="30">
        <location>
            <begin position="419"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="30">
        <location>
            <begin position="456"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="EMBL" id="CAD8061662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="485" mass="55539" checksum="AC9A00022307515E" modified="2022-10-12" version="1">MGPCSSQQRKPAKLPIAGKNSGAQAGADEFKVGPDIFVTLKQGSISNFYKIEKSLGSGAFGEVRLVIHKSSGCRRAMKQIRKDKIIKDDEENMFSEVNTLKELDHPNIVKLHELFQDTKNYYLITEFLEGGELFQKITEMKYFTEKMAADIMKQILGGVVHCHEKKIVHRDLKPENILFENKKQNSNLKIIDFGTSKKVESNQNLTKRLGTPYYIAPEVLKRNYNEKCDVWSCGVILYIMLCGYPPFGGQDQEILQNIEIGKYEFDPEDWNKISEEAKNLIKKMLTKDYTQRISAQEAFNDPWIQKNAPNAPIDFKAIKNLSTFFGKNKVRAALMQFISTNLMTNAEKEGLLNEFKKIDKDGNGQISKDELLQVYLKQYDEIKAKQMVDDIFDKVDTNKSGFVDFTEFITSAANEEKLLNKQRLQQAFNMFDTNGDGQISRDELQEIMGGIDDNLWKEILSMCDSDGDGQISQQEFIEFLVKKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LA29</accession>
    <name>A0A8S1LA29_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0360251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LA29"/>
    <dbReference type="OMA" id="PNFYLQH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024453">
        <property type="entry name" value="Peptidase_C92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47112">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47112:SF1">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05708">
        <property type="entry name" value="Peptidase_C92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8064688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="292" mass="34767" checksum="E798E64F967879DE" modified="2022-10-12" version="1">MKQQWNDINEDFYDHEGLLLKDKIIYRGMLRNYTFVLQRKDKNEFILDFNQYPIKLSILSNDVFQLSVFSHQFQFQSLTNSKQWILSLNYYISTSKPQCFQHPLPNFYLQHQIPFDIFEKRAETLDLLLFKNKSIACQFQRFITNSEYDHVALVLRNNKNVLHVFEANSDDGVCIYTWDALMQSKFKDHVTQISYRQLYTKRDMTLLIKLQEFVYKNHGKKYSANIMKLCKKRSITGVEKENYFCSELVAACYKHLGIMENDISSCQFWPKDFAGNIKLSNAELSHEIIIYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCF2</accession>
    <name>A0A8S1LCF2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0350249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8064005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCF2"/>
    <dbReference type="OMA" id="WDWLPYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051438">
        <property type="entry name" value="RNF_E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016:SF1">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016">
        <property type="entry name" value="ZINC FINGER, RING/FYVE/PHD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8064005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="24778" checksum="51619A95E6DBAB11" modified="2022-10-12" version="1">MQIQQYQIFSRSELVIHEIVKDFECPICLDTLFQPVKYNCQVHSICLDCVIHLQKCPLCRRNISDVQPNQELRKTLNSLQCKCPQGCGIIQYEDLYSHKINCPLQSDAQKKTSQALMQIKDQMIQILDKEINPHLKEMHRKIFDDFLTNWDWLPYKKEDWKWWWWSNTAWWNQQTCEKCNELWHKYEDEIILYENIRINLLELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LEL5</accession>
    <name>A0A8S1LEL5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LEL5"/>
    <dbReference type="OMA" id="FACKNQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="257" mass="30931" checksum="E2DD6DA595322700" modified="2022-10-12" version="1">MNHLLIKCEDQQALNGINIPKQISLFLDHNHTVKYLQKEALIIQKFLKALHNLQVAQSQSHNTPKYPFVIFISYNQFVVHFGKTQLDINNMFRFSFYPQSQLIPQYNQVNSQFPKIKLQIYKRREVRLMQDQKDTKNIPKNYCKSIITFACKNQQNLCIEILKEQLKVVKFIDKISQYKKKQLNIRVFSTLLQKSEDPEEEEYRRAFRIISQIFIKKQAINYIFNSKIVQHNWHMKYRYQIYKGIKNPQNFTHVKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIB0</accession>
    <name>A0A8S1LIB0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0400275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIB0"/>
    <dbReference type="OMA" id="QIIRSYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="537"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="622"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="554"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="568"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="661" mass="77063" checksum="62AAFA732690DCC5" modified="2022-10-12" version="1">MLKFEAFKKQQRLIERSPQFIVQQLLQRKIVFIRENFSSKENFMIQKEESQEIIYLKPECSDHVMNELTRIAMSEIPENEQLEYHIRAQQLLDYFTQNQVQIQYTTVLFHKINYALALRKYGMIEEVINLLLDSDQILKIKGKQLKKQPLDIQLLAKIVQNKLMRLRIIFQCTLLFSESRRNKQALELAKSALKQLKVIFDTTIKLCQQMNLTNLINKKQRANSEMSLATITSQQTQKLSYSQIVEMVFKEILVLIKCSMPQSQFEDKNTQIIRSYQSRSQSLDKYSEQIQISNTINQNNKLYPNLNDNHPMLQMQILGLMQMTQVDIEELYPINNPEMVVTEEVILELIMLAGLSFYSISTELRFINSNFDNRSNLEIWLCKSLEIYYTYVPHMSQIFNQIYQVYKKLYGVDKQMIPEDQEITHLTKLLRPHQFNNKATLANKVVIVIKVPDGPGSIIKHANTQQKTIPSNKTSKKHLLTQVEDQEDTGRSPSRLFLKPTKSQQPLEIKQRVEFLMNHILTQQAKLSKEKMKIIKSRVKPDLSNGNVGMTPSTNTTNNTTQNTTQNSAKSPPKQLSRSLSNSRKASQCIVPAQLNIQQALHQTRTAQLKYRYRNQFNTLPIEESPTQFSSTRKQGTLDSAKKQYQNKYQLVLQQKLQNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LK00</accession>
    <name>A0A8S1LK00_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0390275</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LK00"/>
    <dbReference type="OMA" id="FMSMELL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001631">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="20"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8066745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="329" mass="38821" checksum="B51755AB960A0F65" modified="2022-10-12" version="1">MARSQKSLSIYRENIAFSGYLIQNKINKGGFGSIYKGVNKSTHQEVAIKHSQFDLKNEYQIMTQLTNVVGIPKVYGFEEQEKWSFMSMELLSQDLHTIKCKLKQFSLKCICFIAIKVLEILKQVHQMNIVHRDIKPTNLMVKSLKDPQIYLIDFGIAKDQSKGDTRQELEGTFLYDPISVHKRQKYRFLDDIEMLAYTLVFLYNGHLPWKKYGNEVLVNGDALKQKEQYINSKAMSQLPFADLFGYIKWNQHIKKPDYEFLINLFRNILRENKIIENYQFDWIEQATPQMKRQSKYLMIEDLNTDLNEEEQQCQGVSLLSELVYKFFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKK0</accession>
    <name>A0A8S1LKK0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-related protein Rab-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Small GTP-binding protein rab1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0400226</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Protein transport. Probably involved in vesicular traffic from ER to Golgi.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LKK0"/>
    <dbReference type="OMA" id="PRGHTIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001018">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="188"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8067329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="224" mass="25812" checksum="FFA27A36B2922500" modified="2022-10-12" version="1">MSDYYVQQSSECDYLFKVLLIGNSGVGKSCMLMRFSENQFTNHFYNTIGVDFYFKLINLLSNCKQQTYIIPIFQWDTAGQDRFRTITSSYYRGAQGIIIVFDVTDRESFNQIRQWMQEIDKFAAESVNKILVGNKIDSSQRRVSTDEAEVLAKQYNISYIETSAKTNINIENCFSLITRQIIQRVGKPNQNQKSKVGMKLQQNSPQQQTQNKKGGEQDGQCCMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LL03</accession>
    <name>A0A8S1LL03_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichohyalin-plectin-homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0400047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8066971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LL03"/>
    <dbReference type="OMA" id="WKNDNAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019">
        <property type="entry name" value="KDA ANTIGEN P200, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="516"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="565"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8066971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="638" mass="79088" checksum="E94F8E0C35FA1D13" modified="2022-10-12" version="1">MHITPVSLLNFDQTKVRDPIINSPRSIEACRRQGIRPEQLIMKSLYDLKRENKNLNLDEQAWKIFLQHEEDKRQARVQVLIAEREEIISQQKDEYENEKFERIDLEKERKRMEMIKNRRQKQMQKALDEEIKTQELQNQLRKKEMELKERQNEQRQELEQRKKDKKIEEQFRKQQIEYQEHLRKQKLYAEQDRLKYETTHKITPQQRIQQEKELKKQKNKRDKQLKKAQEEREEKERVHQQMLEQLYEAKLQKMEQREARRQILLEEEKELRQQLIQEKQEEANYRRDENKRRAQHQLNQVYLNFQQKQLNAEQKLIAKQKEIELLNKEQDMKRDIQKLKRQEIQKKNTAQQEKRKDDLMFKFKEIEDRFKINEMDRELTLHQKRQLQEEREHYRQQLLLKNDQELEKKCEKISMQREAKELQALKVLKERDDMIKEKHLKELLRKQEQEETQERMMRIRDYEREQLKKKVEEKSQRRDQLIKEKIASEKQKQEYKKAQIMEKEQLKKDLAELLKEQQQQEPKHINIQHKKRPESAPQKGKALQEQQQKNKPIVMAPYDIMTKEKQIQELKIKQNQELLNLLQEEENKELDRQKLLRQVDDPIERMRLEKILEFERALTQDRLAQLDNKHQKELEKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPC1</accession>
    <name>A0A8S1LPC1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0390052</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FQINCEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053095">
        <property type="entry name" value="Actin-binding/GATA_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23246:SF21">
        <property type="entry name" value="ACTIN-BINDING PROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23246">
        <property type="entry name" value="NEW-GLUE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1046"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="117"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1055"/>
            <end position="1070"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8066366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2191" mass="259104" checksum="E98F8DB22CFDF649" modified="2022-10-12" version="1">MQDISILENLLLKPQEEAFKHLVKGSDEYYYFYFLKLFHEKGANLSNEEKEQLKEFISRDTELSEKIKKRKLFAQLDYYSNDVIKRNKVLGLLREYVFKIEYNNDVDPQIYYLSDVSREEEYEIEQVQEQVQETIDQNEQIEENVEIVNQNQESQQLENIDAQNDIENIKPSYIEQEIVQQEQEVVQQEQEVLQQEQEVVQQEQDIQQELQIEEPQQNIDDNDQDQESNQIIEQQEQEEELGYEKQYPSQFEQCLISLNTYLERVYTYSSIYLSWLQPYYYHLLDIDRILKQNSVQLIETYLKKAENIDQNMDVIKLFQFLLNQKQQSKKLNEFLPKLSESQLIQLSEFQPKLLDDKDFVMILYQIKFERKAIQLNEDKQALYKLKEASTEFFFKLSPKFQDNKIQQLNSLIEFGTSNNILIKQELIIEWLKNYQSIFNPLIDDLQVNENKKIEYLVGNNTQFKILKKYLQTYLRNNENLSFLSQYISNNILEQMEAEIKLYQGKQLEHISKILPQDKLEKMNNEKILEILDTNKKSFKHGEQVSLQCKLKNIQQLTIKIFQINCENYCLLNEKNANIDISLDGIIPNEEIEFTYQQPPIEIFYKEFTFDTITNSRYGAFVIEFLGSGLYAKAFIVKGRLNLENTITSAGHSLKIYNENKELCKGEGTGVWLEKKFYQVNQRNEIMIPFSINRKVINCIIVYENYAEVSEITIETEKYELNCLFFLDQEQFISETETSITIFPELTVANKTIDIGLLKNTKIRVQSTSISNVISYNQTEEAQFKNGQPYSFQMRNLSNLDKTTINVFGQIMSMTENKLLDLQFDFYINYQYESRLSKQYIKHSQEYGYELIVIGRNGEIYENLLFEITFWSMYSTQSTTAKLKTNDQGKILLGQLEGIIKLSSKCLSSNQQQVPPDLNYTILKTDYLKNTIQIQHLKTIENQFLQTISFQIQQTEEKYQVMVIASQFIHPNSQNIEYAQYLQKNQFKKNENLYLNDLIDNQFKSNQYVQFEIAYVQNRRTKPAYIGNTLEKPSVLLNREFVQETTNVEECQSDNQNEEPQEYGEEDDEDRCSDRMECEAPYEKKKMKKKSSKRKQIAGDKGIKIQKQIEVTNHMNFLKRSAQIIIVKQQESNQYELTFPDYIQQVQIIVINEKNIQNKTIGLKSKDPYQKDITHKSKLEIGKFYSPYRESIIISKGQEYLLTDCANTQYKIIDSYEEACSIMKNISQNCDHSILNIYSTWNNKNLEEKLKLYNNYQSDEFNLFLYFKDPAFFEIYILGYIRNKIEKSFIDQFLLKNVQYLSQQVSSGRFTQLNALEQTLLFIFFNEIQENKLYSNRIKNCLYSTIKNYEIKQLERKQTFDTIIGGEGEEKGLESDCENEDEDYQGAGGGGGNAIECCEDDCYYDCEQNYCEEECQYQSKEVKRGRRTLQRHSYNDYDDCNQYDVYREMSIQPFRNIKQTCEYCELHYKDRQTLQNPDLTTYNDFYHDLVEHSISQGVIDNPFVSSKFIYNNLGNNHDLIITAALLGLPYTNLVQVEQQYIEKDLHIKAQTPCILLKKQLKELPKQINKQILVIQKFYDDNNRYIKKDGENIELQPQEFEIKKIYQCEIVITNCSSKKLNVFLLLEIPNGSLPFDQNNYSSINTVSLKPYQSRTYHYSFYFPNIGSFSVYPAVVSTEKAVIAVAEEYQFIVVASSQILNKENLMDIIQKGSKDDILEYLRNNSGVDLSQIKKYFKDRTFYFKCCEILREKLELKEEILQYCFIHNDLEGLKDYLALEDIQEILKEQFVYLKTSLIQLNSVRFYEYYPLLVKRVHTLASIDQGILNVQLKQQYKEFLNYLVIKQDLSITDKIIFCYYLLLQSRINDAIKIYQSINQKFNEDDPILQYDYLSAYLDLYIGYPNFDGARSICEKYLSYPVIEWRNLFYEVMNLLAEFDGDEDKIIEKQTDEHQQKNQDQANKEEQLQFVVENDQIKITYSNLHQIQIEHYLIDLEVIFSKNPFLSDQNYNNYTYTKPIHTSKHTLQFNQTQQQMIIQFPDNLKHKNQIIQIKGDTKIVSHHYIETKLQINITENSGQLRISNQEGKYLEKIYIKTFVKKSDGQNIFYKDGYTDLRGRYDYATLDSQSLEEVSKFAVLIVSNDLGSMIKEINPPSQVGNYGREVKLQSKLWTGKAKMEQEKQEIIYKSKKLDKKKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQU5</accession>
    <name>A0A8S1LQU5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0450087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQU5"/>
    <dbReference type="OMA" id="DFIEGHN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="40823" checksum="DFF08A34693B8505" modified="2022-10-12" version="1">MQPYFFQGDQQFAFQLDSDIQKYMPYQNYNEFAYLSQFSYPATFPGYSVDCFPDNEVIHQQKYKKNKKIKNTDSLGLLPKTLEFAKQTVEEPTHSQLQIDQISKQITPYSIFHCIQGAPMPKLQSDIISNQEAQGSQEGSQQTQKQKGQHQSTKKPKKEHINTGHWSTEEHTTYIGFLQQYESIMTSSMMKKTSKIFKQMSELIGTRTPSQCRSHHQKFNPYALRGENGKRLPRTERSRAGRKKKNPSSDIPKAEDVNIQQHHFDQEQLIMMYDKHLLHQIPYEQWNPYYDMHQNIKKEEDQDQEQQNSQQIQLNNQQEYEEFVRLNYQKQLDFIEGHNSIVNEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LRM2</accession>
    <name>A0A8S1LRM2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0430077</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8068905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="RESFAYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07199">
        <property type="entry name" value="Pat17_PNPLA8_PNPLA9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000065">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176:SF92">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="3">
        <location>
            <begin position="1866"/>
            <end position="2062"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="458"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="1">
        <location>
            <begin position="1870"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="1">
        <location>
            <begin position="1902"/>
            <end position="1906"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="1">
        <location>
            <begin position="2049"/>
            <end position="2051"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="1904"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="2049"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8068905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2195" mass="257828" checksum="E2D884C9134D07AB" modified="2022-10-12" version="1">MKQLEQFANTFYSLLQAQPQKLSNFRVKLKFILSQLKYQYSDILEEQQKLVKKNSNDSNILQSLISNLQQTDKSHHQIEQNELIFCNKLQDVLKKFKKPYIENLKDIAQQVLISDLNQLQEMSKIIHSISPLHEILLQLRNYISQENTYYEDKALLKCPLTTIHAINKETAAQLIKGDQNLISILENQGYQIKRLKNNEEIFYYQRYLVSYVLRSLIFQLQLPPGFVMRVSTYEQKNKELKKQYSYFFITEKVAQSDLVINKIEFAKHYLSLQCTDHLDSLIPQYYGNLQASFQKDIPQQQTMYEQFKNPLLYYKKNIFEVTYSPQEFEDFKPQNIYENFLNFLSFLSDSQLNDFHYKQEEKNEIIRYKSLQITQDMLQLTYQRIKKLEDTLITVQEVIFPQQLCVRLIQITEKLNQVFFQFQRLDTSRFQTLNEYQEYLSKINLEELIHKIIDQKNITKYNNQIRQIKQDLNQLEQQHEKTQGKQTQNSNLLSQYYLQGPKYIIINCCQKIDIQYEISLTNRIKLLDFLANLNFYLDTQFVSQQEYIYDSIIKGFNDSWFELDLWKQAFKANYSIQSQCQFNNTLFVELLAILFFKENYTKLNLNIFESLEQVQENEKNSHILISYLMNKSFKGDLGFGKHITLVESALKHKKFYLLLKLLENNQGQKIRFEYFNYLLDETQNLFSQSAYFSNLSENDTKYHEQIFDQIAKIHPQLEGRLRWDHFWNQIVKNFGEHTLKILGRGFWSLKDEQYKQIFINKQLGNQGEIPSYLELSLIAQINKNNNKNYSVLKVNDPFINQLLFIQPNFPLQKFMASQLYQLLQLDQFSSRVELIQSEQQIYSVYQVINQFNNNQRSKVTIAQFLQHNEYNEQRLRNKIGNYNLANKIVLCILLLSTNTSPDEELLVPCGNYLSPVSTNFYFNPQYDNFINSHGFERDGENVKLCCHNIYFLMQFMKEKIDYQLLQNFQNITLTIQQWLKQLSQIEATASQSEGLLLLPLYKENMIELVRRATIIQNLITKPNFHITYLDLLKLCDKNLGEFYQILIENHTLNLINKFVELKHLSTNSDSLILSDFITFRESFAYMKYPSGFDVKNMIGPYKGLKMFLKYSRLLSTTTLQNFHKKLAVIKKQQHFQMLMKSFEWRNFKENEIQQAFSQLSEYSKDFNKPFELVNFKDLQSKQFPQLFQSLQLIRIKILNLSYVDAVNDNFLFDIAQHCTELHVLNLSGCQNVHYIGQTTNILNIGNQKPLQFQRLKTLIAQNLPNVKAIEISGQFLQNLNVNYCKNLQTVYTNKIKLTRVQAKGCGQLQLEIIQLWIIQGVRILFDEHTSQGSLKGFVQPQIIIDVDKEFNYDKIYLEIIFQLLSDLYDKILHQDQYIKSPFPQFFLKNGFISDDFEFQFRGFLQNKFDEQNKKKILQCLFICLFQNEQIKQLDLKSMLKNRSQERIKMLDQFLNDFVQAVHLTIEEQDQKIKQTAQQFFLNVESLTIPCSKEEIIEKIAQLIRQIQNYRTTLNISFTNLKVIGNDIIGKFFQKIQRNDKPIHIQQIDFSGNSLERENLKNIFQVNNIIQVNIQQLILRNAGIDDNILLEIAEYFWECENLKLIDLSQNQLTKKSLNKLFEDGRNLAKIPLNNINLSGNKIGDANFHILAEIEEKQWQNKEQNMLYLKSTILKNCFDETQDIQYFCDFVAQSYMLQILDLRKNSFNEQGMELLSKSIGKSKSLTTLYFQNNAKSSKAILVQEIMKNTHLNSLEIILQHEEHYNEEFLKIFNYSSLKTLELTLPTITEEQMKQLVELLKSNISLVKFRLNINNNDIKQRTFYKLGLSKIKNIMEIKEQCNQDIYFNDPFYRQDTLRIDVQDYCQMGLSIDGGGIRGLMPATIINYICNEIKKEPYQIFDCVGGTSIGGILALAMTGTQDGVHPLADKDQLVKFFTEDGKIIFDQQKRGVWSLINKSKYDAKGIESVLQRYAGTAKLSETLPHTNVIVTAVKLQKHKGDNMAKVFSSRKAKLDLTENFLIKDVGRATSAAPTYFPAAQIKSLAGKEYQFIDGGVGKNNPANLVLDDLRKGMLNKDKDNFFVLSISTGVSKQKQHLQIDEGIMGVVRILDAFGESNQDFVDLELKKNQGKYLRIVPEYNLPESQAQLDCTDIKILEEYQSAATTAASQFLEQEKFGQYQDKTFIRWLEENTARRLESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LY01</accession>
    <name>A0A8S1LY01_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0450217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LY01"/>
    <dbReference type="OMA" id="GIMSYTH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="323" mass="37676" checksum="1502DD197C70ED0C" modified="2022-10-12" version="1">MREYKRNPEKYIYMGQGITPERGGGPYTLIHTKYQALTRAQRKRHIRTIQGHYFTSEFASEDVAFTFLLNGLSNINYSDDFEPSYEYMDFLFELDNVIGVPQRAMQKTKEYNDLYVKNQVVRTMVESFNFAGSREIARPFESIKNQRAIVQAYCNSDRCSFTHPELAYWDRVPEELINKIATYYYNNHPHMKYMLYMYALMARRCDLDFAKQEVGLDSTNPYFQAIQKGRAALMKILDQLGLGDNSTPMIAIWKQHLGCQLQSMNMEVNQKFSTFRTLNQLQLYQAKKMVKQGILETDIIACQRKLRILQSTLANMTSFAGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M357</accession>
    <name>A0A8S1M357_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0490025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M357"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="117" mass="14583" checksum="5D62D6F64A991350" modified="2022-10-12" version="1">MFEISSLIQNGIQKEEEDKQVNQNYICEIMRRKQMHFHQKILDIRRMQSQMLSYRKKWNKEYQMILIFEVCMQIKNKMYQNRKQTNYYINTIQLDCSVEVRKIRIKLEIIQKKMLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3I0</accession>
    <name>A0A8S1M3I0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribulose-phosphate 3-epimerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0490091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3I0"/>
    <dbReference type="OMA" id="CHLMIED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016857">
        <property type="term" value="F:racemase and epimerase activity, acting on carbohydrates and derivatives"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000171">
        <property type="entry name" value="Ribulose-phosphate 3-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004076">
        <property type="entry name" value="PRK05581.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="RIBULOSE-5-PHOSPHATE-3-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="22407" checksum="1C9F0561A44E38B1" modified="2022-10-12" version="1">MGVDWLHLDVMDGHFVPNLTFGPPIIKCLTNRLQNRTFIDLHCMIQEPRKWIKDLSNAGGHQMTVHYESDIGNIEELSQMIKNAGMRPALALKPKTLIDHTLIEIFDKQLFDMILIMTVEPGFGGQKFMADMLPKVTQLRQRYPKIDIQVDGGVNCDNVISCYEAGANVIVSGSGIVNAKDPQQSINYMKECGCQHFKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M786</accession>
    <name>A0A8S1M786_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0550166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M786"/>
    <dbReference type="OMA" id="FHENECK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="174"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="367"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="410"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="520"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="575"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="626"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="677"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="927"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="1025"/>
            <end position="1066"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1104" mass="124879" checksum="5A72ADA26FA9D5C5" modified="2022-10-12" version="1">MFLWPIRLLLISTVIGFRLYNFYKYFTSQYGYEQELCPDLHTHVFDMHPHKCVSYASICQGVTEIEVKIAVNGSQYLCHPRFQPWGAGSPQKDLYAIHCGYDGGDQLVMEMLSDGHYKYYCKSFTDDINRRCLITALQQGLYRCQFCKYPFLGYLCKFKDSTDFDERYTNPPILCKAIYCAECDQLEACSKCPYGYSLIGAHCSFCDGFTSCIWNGTHNIYNQECIDGFSLRDNKCMYCMQCKSCVNTSCDSCYNYQFRVLKYWGSQEEYACAYDVSSACDFSRQSISEPYLPINTINTELKYYNITCSLCDPGYFPVSNGCELVSAKFKNCFMLNLNGDTCQACLKQYALHSDGTCQLMQLGGSSNCQTFLDINPQFCTTCDATKILIQESGICICKPTYGFQVDICIPCTDGFCQECDQSDFYSCISCKPGSNRILMDQRCICQPGSYDPENEDQICIICDQSCPTCFGPSNQECIECLDESISNRIQIGDSCPCKTGYAEYEIIESKCGKCHPRCNTCFQAADETTNQYCLTCKPGQNRVVSDNFNCNCLENYGDYYGTKDVCFVCDYTCGTCDDFGPTHCIECLESSNRYLTSSGECICKTSYFDDETDNIECSKCHYSCILCANSIQKDACQQCPLTREPSDPLATQFECNCSSSNLFDDGFSQECLQCDYTCQTCNGPLSSNCLTCDSTYRQLDLSSCVCLNGYYDIGQLQCEKCHYSCHQCFDNTVEGCIACSLDLHQRVQKGNICKCIDGYYEEPGIALCKQCSYKCQTCETQSEQCLSCPLNSLRVLDSIKGCYCLGEYYEKENEIACQKCHFKCKSCNGQNENNCLSCDSVANRELKGNECKCQIHYFEMEVQECTICSAFCYECINNFENCTSCNNDRFLVGSTCKCITKFNGAAISTFDYNGMVKCQKCHHSCGTCGGIEELDCINCIDTDHRYQIGTTCLCNEGYYDAGLPVCEKCSYKCKGCQKQSESCVSCPDNSFRQFVSGFNRCQCIQRYYDDGQNEVCQKCHYSCLRCNDIETKCELCSIESNRIYNDQLFTCDCNIGYYDIGIENCQKCHYSCLSCNSGDFNSCISCVPMNNSNRVLCKTLLFWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M868</accession>
    <name>A0A8S1M868_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0560135</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M868"/>
    <dbReference type="OMA" id="WFLIQLN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044613">
        <property type="entry name" value="Nep1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468:SF1">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="233"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8076460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="432" mass="52144" checksum="938745D324BB2600" modified="2022-10-12" version="1">MLINFQILITLFLILVIYFLTKERLIYYYNQYKAVFKKLTDDYQDDVANIQYIKDYFPKIFQKKNSPLLPSQTLKTEQPKFIIRHYEDFLVGYSQNLSYQIKCKDREILYGQFFNKNFKAYTGIGFSYKVSYDSNFNPKYELYEGEFKNGLKHGQGKLFGSKSENVIQEGVWQQGELKQNQNNDQNNNQQLQQNDDHSTHFTIIIKEDDKIIKSNLKRRENQRQIEQWIKFNQLISQNDIKILKSKNRWFTSSIIDSFVLYLNIQIDEQYFKIDFNQRKQALRQIFVPSYVFTQMKNNTKDINIQIFKNHFLEYKKIDYQIEKIYNRINFIVNRNNTHWFLIQLNLKNYQMIVMDSIIYKEESYNCFKDILNTIFQQQITKIVISDQCIHQNNGYDCGPYTCLHMLKLSEIQLNLETPSEIRKFLLEQLKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAI4</accession>
    <name>A0A8S1MAI4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0510134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAI4"/>
    <dbReference type="OMA" id="VELPHFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="469" mass="55499" checksum="939F3FCD0C0AB98D" modified="2022-10-12" version="1">MSQKKNDCELILIDPQDETDIKPMKILKKMVSNAGGIRYLVEYSNNEQKQETEKFMKENYQELIEDYNYFSVCGSRFDQQTLQKMRENNNKMQNFENHAKLPVIPSDENYPIFKRQKKIEKSNEKNNKLNNNQILPLPQQIQQQLQVQQQQQQQQIQLQNNLNQNEKYLIKQQQIQNQTLLSLKSDQGDKIKSISLENGLFLVEWLPRPDGNVPYQEYYHYDFLKATAPTILLSFLEQEFLNDDFKFKNLKRYSFQNTNKETIQNDDNQQYKDKSINIIQPDKSQEIIEVQKEVLKDDHIIKRAVQFEEVKEQKDNSKDILIDDFSVDDTQEVILDEKQTAEQEKKQQQQKKGIIPSFKPQGQTQKQRKQVLDKGQELYTVDKVQGKLDEKLSQMEKPIAKQQVTITQPKLTNQSFPAQFAQAQFSDEFEILGSLEKPEDKQNQNYEKELEECYSDVELPHFQFNQSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCA6</accession>
    <name>A0A8S1MCA6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0540140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCA6"/>
    <dbReference type="OMA" id="IIAYRTY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="VPS9" evidence="2">
        <location>
            <begin position="187"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8075313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="317" mass="36884" checksum="8C7C92A262E386B1" modified="2022-10-12" version="1">MGNKNICCCTQEEQQQQSQTLGSELKLFLSFSWHAQVNEIINRNYNHMPLLNEYRVSHTLQTIQQRFHIRNQMLSDIFYSFVQNPKEMKFHSLMKISFVSTSIIISDNSYQSQLAQSFMKATDCQILQSLSSSPLDLKMEINLFKYFFVDLILKFYKSIIKIDEIKMLILDELFGNQIFMTLSNYNMTKDYIFKCILNVDQTIVIAYQKNIKKQIRSQNKQEKAVAILQSIITKRSPSEKLIVLNKATQQLTSQSKIINDDKFNELVNDLIVHANIPSFITEIKMIHHFAGEIDCLGRYGYVLTGVMGLLMLIIQDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDS8</accession>
    <name>A0A8S1MDS8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0550011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDS8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="77" mass="9371" checksum="D15D339F75BB04B1" modified="2022-10-12" version="1">MQKIESSYIKSNNVIEREKSQIFSMYQYKSQINNINKPIDTQPQQKFGYEQYLILIDEQEKDLFKLSELKNQYSRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MHZ0</accession>
    <name>A0A8S1MHZ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0620181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MHZ0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="203" mass="24421" checksum="29B5627B594D03FC" modified="2022-10-12" version="1">MNQLLKTSSDLDSINSEFNIIIDKISNGIHLNDEHIEDDLKVLLEKLDNENKKEKILFLHKNFPKASFLNLPYFRQININQNNTQEQIDGYKQMYQDNLNEKQFDLILQELKNEQYCKQNLIPFQIKDYLIPLKQLKNNIHLIFELIEIVKDKTQYNFFSIQYSLLNCSFLNVIMKQKDKFINQQVVFQKIKYINLKIIFKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPD3</accession>
    <name>A0A8S1MPD3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0670189</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPD3"/>
    <dbReference type="OMA" id="KINIYQR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF9">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8082308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="224" mass="26809" checksum="A7860CFF9ED550B4" modified="2022-10-12" version="1">MLSPLIISTLIETTIMEGIFSTCIACIFSMKMYSYIVNEEKLDLIQYNQYLWIPCLVCQPRKMVFKKQRNFIQIFKCIINSTLIILWDFYYITYEIKSNYMEDDLVKFIINYSFRLGILIVVNHYLIFDYLLGLFKEITKAENIIFYEDWWNADGFGQLNRKWNKQVHNFLKINIYQRLSKNGQNTKALIMTYLVTALLHEYSQMFHSKLQDCIIYPLVFSNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQK5</accession>
    <name>A0A8S1MQK5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0570251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8077174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQK5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="133" mass="16012" checksum="30ECB05F3C6B0194" modified="2022-10-12" version="1">MFQDRNPRVQLFQFIIRISLKPDSTNGQYYKNVVEFAKPLTFPDEEQDFYLYSHRYPYGLKTNVSFSIGKVEESMKTDQLINFNFYFDYKPTINTKESVLQNKKLKSQNSISFYYQIQENQYQRCGYKKTKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MR41</accession>
    <name>A0A8S1MR41_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0660187</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MR41"/>
    <dbReference type="OMA" id="NSEYKCV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050935">
        <property type="entry name" value="Bromo_chromatin_reader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880:SF225">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN BET-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880">
        <property type="entry name" value="FALZ-RELATED BROMODOMAIN-CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="2">
        <location>
            <begin position="25"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="3">
        <location>
            <begin position="133"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8081822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="25631" checksum="6FC50626FB677291" modified="2022-10-12" version="1">MIKENQTPINKEELKKLNQILQQLIDSNDSLEFRQPVDHKTLGLHDYLSVIKKPMDLGTCQKKLQNSEYKCVEECLNDIQQIWDNCKLYNGPSSWITKLSEKLEKSFKKYVKNYLPLINIPQSSIKVKKVTEEVVQEETQQTISHNEKVEFSNNLKQLNPDQIGALVKIIQTSSPSAFVEVNKERFQIIIDNIDFDTFTKCQNQIQSWMNGEEINKKVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTI7</accession>
    <name>A0A8S1MTI7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0680116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTI7"/>
    <dbReference type="OMA" id="LEYKFRH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="335" mass="39393" checksum="0BF0113D13A039E1" modified="2022-10-12" version="1">MNMIYVNQAQKPKQSIAKRSSSGTNRSPFIKTHEQQAQYEPIPTRQEQIYKKTCEDLRQKIKTFNQLQRCNIIIFGPSGSGKSSLIRTFNTSLNQTQTLPEALCIKDLLHNEGTKHFQSINLLQSTKNQIFSKMNSHLIQNCQINILDTRGQINLSSKENKQVELMLEGKVKNMSLVEQRTYRYAYKLWEFWKQDCELFPQEIISCGTYIPHQVLIVFDGSLDKVPNGKEEIEFYQQILSLVKQKGYDNPFIILTQLDKLEYKFRHHDDYQLYVDQRIESIVQSLKIPRESVFFIENYHGQQQSSELDYQALRLLYECLQQASKYQIQSVECKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MUA9</accession>
    <name>A0A8S1MUA9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0640070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MUA9"/>
    <dbReference type="OMA" id="INMGDEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="183"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8080643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="234" mass="26946" checksum="9A6A2DC66268D89D" modified="2022-10-12" version="1">MSKIQKQNTVQLNLQNVQQFIPSQQIQVGSQIGGNSPKPPLGSKSNKSPKGDCSFRVISPKMTINLNNSQRSISKSSSPIEKQISEPDKLQYLENENDAVIEDNQINTSDVQQIRQIKQKLMNMGELISSFHLVDINLKVLKKWQYELLQETKTMIKLNKNVINLSQTKIISPRPNQIKKISSDELLEKLQNEQKKRLEAEEQSGRILQDQEKYIQVLNEKLFQLEQQYSKKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MVM7</accession>
    <name>A0A8S1MVM7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0700072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MVM7"/>
    <dbReference type="OMA" id="TACIPMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="528" mass="62276" checksum="9319884ED8F91E0F" modified="2022-10-12" version="1">MGCVSSDEKQALAFDDFQYLKTGTLSIEITQLIFVVNNLLKEVHQLYKQINSNRIILFRITKVSLTANQTLIDAFGFWTRFVSICNLGQGKLNGVFYKLDEDGRNLSYNLGLNGDLKLQWFGEVLNDYFKAIIDITACIPMILEQLKFLQDKINHLGSNLKEKNQVFQYNMLLLKFNLNFTKIANIRVVKYNEEIEIFKRNYLQISETADEQGLKLYQKFGAKQLNDRQKYRMQIKINLIMEQCFHGQMRSEIEQDIQNKQKEKRKTLKPSWTFGDDIGIGVKKFPYRVRWTGCNCVDHSFFIISNYLENHSKHLLLLRKLSVILRYLTQSFKTDEDTISRAWFIFCTHLTSKERTIIQSNSSILNGLKKLKLKNSEAEKCRQYFIQYIELLDQDFLSQLQDEWQNYIEGQSKVLSLWSNNQIFQTIEFKALSVSDKYYALTSMSKNVYALQSYYPLATQIYNQVYQRKIKKGQIFLSELQQIYDASAYYPLPFNNLYTTNKIEDDEAKIKTKEIKFKYAFTKFEQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MX13</accession>
    <name>A0A8S1MX13_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0670013</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MX13"/>
    <dbReference type="OMA" id="FMQNLIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416:SF8">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="69"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="94"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8082006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="241" mass="28091" checksum="3DD17ED1E7E5E438" modified="2022-10-12" version="1">MTDDFFNLDFEDNCYQSCQYQFTSSRDSGMSSDDTHQKNVQIKKKNIAKKYEPIYENNIVYRYEDNPEQYKKIRKKLQNRESANRVRGRQKNYVQDMEQELLDMKKENQQLQMLNAKLQAENVVLKQQVEFMQNLIMQSQPHQSDNTLQRDTTKNSHLATLTAFSVILGFAFLNGGELESKESIMGAKELTNDSIISTPITVTHSIKPFLFGSAIALWLVYALYVVYKVFLKSKLKKKKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MY01</accession>
    <name>A0A8S1MY01_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0620239</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MY01"/>
    <dbReference type="OMA" id="FNEPYDW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001303">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="13"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8079864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="356" mass="41281" checksum="BD1123FE7A3E171F" modified="2022-10-12" version="1">MKRSIKQPASSSYQFLDKLGEGAFGYIWKVQHISSGQIFACKLVKNSMRKEKKLLQREIQILQILKGKKGFTQLFASGSDQHNTYFVMNLMGENLEQYRQKQGNYNKILPIGLEIIKLLKELHSLGVIHRDIKPENFVMQDNKINLIDFGLSKKYIIDGHHIHYRENKGMIGTARYASINALKGIEQSRRDDLESLGYMLIYLHQGNLPWYSISDQDKEIRYQKIKKMKKALKIDKFFGLPIQLSIFMEYVKGLKFEEDPNYEYLISLFSQEESGARLALQYFQPKPAIRLKFIQMQRTSFRSPPKEINSSRKASFEESSSAGSDIQEELQESIQIKNFIVGIKQPADSPIYVKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N160</accession>
    <name>A0A8S1N160_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0760101</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N160"/>
    <dbReference type="OMA" id="QQANKAW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="8">
        <location>
            <begin position="52"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8086290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="472" mass="55029" checksum="843657772498B4FF" modified="2022-10-12" version="1">MGLCTSKEAVSESSIRRVHPLNMENSVVLTINYAKIPNKFKRLPQNKSCGLIGLQNLGNTCYINAAIQCLSNTQPLTEYFQQNLHNHELNIQNPLSSRGQITNAYARLICSLWKDSQQKSINPIELISMIQLWNPFFVMNSQQDSHELLAFLLDMLHEDLNRVKIKPFIEEKTFDQQPNQQQANKAWSDYLKRNRSIIVDLFQGQSRNMLQCMVCNTKSYKFETFMYLSLPIQQDSELIQCISEYLKEEELDEANQWYCSKCKSVQKSKKGIKLWKLPNILVIHLKRFKFTSNYRCKLRQLIKFPMYNLDLSNYSEQEQATYDLYGVINHSGTLHSGHYTSYCKNKDTQKWYNFDDTIIKEIKEKDVQSSDAYLLFYYKNSVDSYERQSEVMRSSLVNSQISIAKQNSVVQINNLIIPNTKLSISKLCVSENDQQQTTPQIFGKKKSLKKNKLTPLPIKKPVFFESDNQKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N227</accession>
    <name>A0A8S1N227_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DBF4-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0780118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N227"/>
    <dbReference type="OMA" id="HYRSQFD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="27028" checksum="D57C67D8D52454FE" modified="2022-10-12" version="1">MESQSTYQAQLNSVLDLKYVFLMKPRCKDSVDTKIAIDYSPMYSEVLGCKQNTVPIFNNDGEPSQPGFILQIDQPVHLANSIHYRSQFDKKLQEVQEKNKDLFIPKPRGTKTYCNVCKQYIEDYLQHTESKNHKLKFRKNKVINLIHSMADEFQKNRSIPQNTPTLHSDSDRCGYFSSIQEDSVEHQDSTQQEPHTIKKIKLNEDVSVFNKMGNSLVHSSKQSFNDKYGRQCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N425</accession>
    <name>A0A8S1N425_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0720097</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Neuron projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N425"/>
    <dbReference type="OMA" id="YYGEIIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14509">
        <property type="entry name" value="PTP_PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000029">
        <property type="entry name" value="Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045101">
        <property type="entry name" value="PTP_PTEN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF81">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE AND DUAL-SPECIFICITY PROTEIN PHOSPHATASE PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01301">
        <property type="entry name" value="PTPlike_phytase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="11">
        <location>
            <begin position="109"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="10">
        <location>
            <begin position="216"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="12">
        <location>
            <begin position="287"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8084466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="441" mass="51717" checksum="09E16DE0975CEB14" modified="2022-10-12" version="1">MHSATQEELKSRAKQNEISSGEEEMLSDSGDECDPENYNFYMSQVDFAALANGINQDDQKGQEINLRERVSTQQLRQEVNQVNQHISGNHQNITSVPNPIKLLVSKQKRRYNYNGFNLDLTYITEKIIAMGFPAENIESIYRNSMQDVRRFFDSVHPGHYKVYNLCEERKYDHSNFNQVAEFPFQDHQAPTFSLIYEFCLDLDNWLKAHDKNVAGIHCKAGKGRTGVMICCYMLYARQFTNAYDSMRYYGMIRTKNKKGVTIPSQIRYIFYFEKALNNKWVPDNMPNKQVELVKIRLIPVPNVKFLGGCAPWFRIQNRDKEYNSKNQFPVKEYKLEPYIEFKLKDIILQGDVLLQFLNQGFFSSSEKLFQAWFNCDFFDYTGVLMIDKFMLDKACKDKSGKTFQKDFRIELHVVEVNQDNKSLQSVQNYTNNQITHKNFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7A8</accession>
    <name>A0A8S1N7A8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0730159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8085115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7A8"/>
    <dbReference type="OMA" id="AWINNIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8085115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="547" mass="60421" checksum="0D11176ED35D00F1" modified="2022-10-12" version="1">MHVQQLQIVHQSIKAIVLVEPQQNNNVVPILIAKIQVQQMEFVLINAKVQHQMIVSVDLQLYHIVIQHRDAKIKWQVQQLLFNCNNSKYCLNALATSGQCYDNCTNNIKSSCICGTITKQGCSNQQTCEDPNASTGNCITIPNCINNFDNSCQCGTTQISVCSESTYCTNVSDSNGVCLQYCNQNQKSNCICGSINKIACSQTQVCQNPNDQNGNCIEKCQNNLQTNCLCGNSVTPFCSVNEHCLDVKQLTGTCVQQCNQFQNNCVCGTSLYSYCSSTTYCSSKQQGICLEYCSSYQQSNCICGNKVKQVCSNGEKCVDPYADNGICNLGICTSQFQSNCTCGTTNQAHCKQNQYCLDSGALNGICFEKCQQNQNNCLCGIDSIDICFSSTYCLLQSKGVCMQQCTSVNNQNCLILSENQACLDQFNTVYNDVTGICIPTCKENGETNCYCGSTEKSVDQKLICQSGFECQNLETQSRVCVQKCTSNKTSNCYCGQEKSQYCNEDQTCQDQNQQVGLCKQSLNVQTADYSRIINLRLFAIICLFNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7H9</accession>
    <name>A0A8S1N7H9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0820041</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7H9"/>
    <dbReference type="OMA" id="SPKQENY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001191">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="168"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="67"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="296"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8088708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="420" mass="49196" checksum="6717ED5EC66A3AEF" modified="2022-10-12" version="1">MIQFDQQKQRTLQTNPEDYFNQDDDQPTETNMYQSLNYQKSHQPQNSQGFLVNQHSKLFDLRKKYLKNREDTNRRSVSIHNKMQCNKSPIKTQQTKRQSSPKQENYQPLQKKKTMPITKNLGSNLRNILKSNYQQQGSYGENPENVVIRKELSQLIEIPIEYQGIDSYSFIKTLGIGATAEVKMARNKELDIEVAIKIYDRKKMNNMHLKNLEREVEILNLIKHPNIINLYHTFENDKSIYLIMEYSSPINLETFMKGRPFKRINEDEAKILFRQIADAIAYIHRLNISHRDLKFENLLIDYNTRKVKIIDFGYSIKIDGKQSCSCGTPQYMAPELVKKSAYDQSVDVWACGVILFKILTGVFPFRGNSEKDLNRKICLGKLEYPSFVSNQARQLISSMLRVDPNDRICMQEVILSQWLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N918</accession>
    <name>A0A8S1N918_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0770050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N918"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8086696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="92" mass="10823" checksum="A9D2F5EAA6D37106" modified="2022-10-12" version="1">MQYVRYYLNQNQIKQKVLTLSRQKDQESLYLRDFFILLIIFSTNEFPDLNSASFEKHNIADGEYYGRLQGSNREGDGVLSLKKKPIQKGIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBC7</accession>
    <name>A0A8S1NBC7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0790030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="QELEGFW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052828">
        <property type="entry name" value="NELF-A_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13328:SF4">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13328">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR A NELF-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="196"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="456"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="595"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="708"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2952" mass="352074" checksum="9A16CA358DED1F93" modified="2022-10-12" version="1">MYGLNHQMNRSLDDNDETQTLEEEVMDDYSPTKQEIRDYAIHLGMNLPEDNQYLDIAKAGLKAKLPDEWKICSKRVNGNEQVFYKNRETQEIVYEHPCDLIYQKKYEEAKQIDFQNQKKKKSTGINKFKKDIEKYREPNYLDGNEEAESFMVDSNVYYENRQNQGYVVQNPIQVSATQNLLSQVDQEFAKQAIDYEDEKKKDVIKLKEENKQKLNKMELILMNEADPELFEFKEEYERQKENLSKNEEEKKRKIKRKVEEEMEQYIINQKNVLQKQLERNLENYEYEQEKTIKNKLRILEQDLQGQQKDIIKRINQRQKELSDSDSKWMKQIQEKKKNVENQLNKYKKELTQEIYQKIQLIDQENIKRLRTEQEVIKRKLINEFDKVRREYKFEIVDLQEENQRMMRSIKEEMKSQYDPKFSQIQQIFSDQLQNIELSESQMLQSQIYDYKNLDIESRKQENIKKINQQKEKLIQQLQNQVINLERQTNEKYQEMKRKLEETQRLKLEKIQKDFEKKTGKILGNGKEIDLCMEINQTRSLLHELKEEIKQFKRKKIEAEEKLASMNIVVSKYDLQNELKQKDVIKFELDRLQYLVNQYKQDDKIADKELELLEQQYQQMIRKHQEFQQKQQTMQNMIQSVNELREQLAFNQSSDNSKFQSNLFRPNKNYGTEINIQNNLSMNQSVVIEENAVQMRKWKLILLDERVALNREQQLYKTDKEKIKQMRRKLLIDSEQLKSQIEMAQSFDQNNMKKDLIKKLKQEIKEQQIKITDDTEKSNSWRQILKQKSKTLDQMEKNINDTNPQPELLHSLEQLYLLYSKIGELELNDDMIEQQISNNNESYFNQIKIISEEPSMQQEIQQQNYDVAMPFEQPKVLHFEQPENLQKYRMQLIQQKEWFQNYKFLQLILIVQILEKDFKGTNSFYIAKYEIIYIIIFIFLKRNEVNYVIHHRIRFYQCKSQMSNSEELSLEPAYYKPMKKQQEMIKQQVQNTKYYESKDGEPSQSFEMEDKLLEASDEYGYGYWVRFSENENQDTLKYYFLSRLTTNKEHGDLTYYGDRTLSVFLYKNVFVFATYDLDENLKSKERPVKLQENINGNWYFVCYSKQKSEIVGFLVKFGGNYILRFEQYGRHKPSRYYKVIFGGSEFDYLSMPGQFANIYFDFDEPAFINTNEDVIKLIQKHCNMPQELLKKTTIRLIEETKDFSANNKEDTIQMEPFDNEYIITQYSVKGWFRWVDNILVEELNQFQIFNLRSNKDKSDKTGDRALEIHQIIGGGSESQIYFSTYSIIEEQIRITKQLKSNQLVWTYAQFSYDIGSLRAFGYLIYPGYAQSVEFNEVQHKQVTKLFLTIGADNEISSFNGKIAYIQLNVGPKSYNDKMLLDDEGALNLYLRSKLVSQEEDIRPSRYNQIQPVFSQMYDDEKVNGQSEYSFGMWSRWIRTYPIYLQKRAEIHSIAHLKRQYKQVILEIQITKQSYLFQSLDEIKNIEYQELEGFWVYLYFGYNRLENRSLGVVNKLGKDFQSIEFNNQHELILDSIEFYVGKHHTPLFNGEFAKIQFQFGPHSFRQESELKELDTQGLPYIIPLNQQTIQLIGAVQQSPIDEKYEFEQFQGITEYSVSGWVKFNGKEKSGEQYHIITLTQRTLEESDDQATLQILKNDVNYVFVTYTCEIECDKYLKQESGFGEYWDQWTYIYFGYKNYQTYTYIEYKFTQNSFIIKDVNHYYMSVFSVLLGQQRKEYLQYTGSLKQWVLNVGNGAYREGSYESEQLSIKVHFGYLAGTDHLTQAQQMKEHPEVLDCQTQQIKLQQSEELQLDGLYEYGYGLWIQFKHYASTINLIKPKLMGIARLSLQNIDHLLSVVMNVGNYQFISLEISEQINYTAYHESEWIFVYYAYQRLQQDVGETIGFIVDNNGIRQIKQSVLHPLIRDHLNFQVGRASKNYIQFNGLITGIRLYLGNGALINEEQLKQMILKLNRKPTQIYIDNVITIVEDRTDNYNGDNPIHIEGSANQYSMQMWFKMSLNQFLYRVTQNEHLSDRKYIGDRVFHLQTQTDNVEYSTYNLLNMDEVDTQVIQQCKIELYHLNMWTFSYQSYSKQEQKMITYLKIDDYICQQHKDNIIHAISDQIWLYLMKDFNDQHSNTMISHIKLTLGQGSFIDDNFIKLSSFIAGNRQMPKIIKEIENVKDSETFIFNEVDSMSEYAVSFWCKFSSTYSERRYRKPEQFQIFRMTTNEQLIEGKIELKDRLLSAYGLKDSFSFNTYDINDNAPNEYSIIQIDRQENRWYFLYAGYKRKLQIAKFYVFDGKDEKQNQNQNLLQDPLIDYIKVIFGSEDHVIGFNGQLLQIAIMIGSGSFGQSGSFLESSLLDYIVQYQIPEEQTLEYLYKQKNGIIELDHEFDQHDFHNVGSYSICGWFKLNYSPLSKYEQETSCQTLIRLYENEKLNDKLIQGDRTMLIQVCDQQFIKFQTYTLNEIQSINEHKLIDNIIDMDSPVIWHYVYMSYDDLEQNVQILLKQFQSDEIRILNNIWHMETNYLGILFGKDIDNINFDGVQQQWQFLYGYNSIFDLNKPFYQDKLPNYYNIKNNQRELWFQSDDITTVEFHNAVNQYAIGVWTRWEAEFYDCKQIQFHNIFRFTSRKQYEDKAQLGDRVLFSYITNKNYEFSSYNKKQKLTAINTQIPYDFIEGEWNYIYFAYNDGSLYAAVIHKQSQKAEHTILRDIEHDILGYAQLIINKPEFGFQSFKGQIYDLRVFLGEGAYLDDTQKVATMINKLHMHLPDVQLKVEEFKWEGCDTQIDEDASLIQEFPYSEQTVYSISGWIWLYSKSEGVLDSLQGVMRLSTDLNFNTLLVLAKSNQVTFGSYSFGKKALPIWQSCDIDMEQWLFVYLSHQDDITRFYVKSEKGVQEFSNVSRHVIPKNFKLSFVKFDSFEKFDGKLKGVQVKWHKPLDEPEDESIAPVLSHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBF8</accession>
    <name>A0A8S1NBF8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0810192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBF8"/>
    <dbReference type="OMA" id="NANNWDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="361"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="2">
        <location>
            <begin position="319"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="420" mass="49535" checksum="FE9B95B9BE699DFC" modified="2022-10-12" version="1">MDSPSLIVLNTDMPMGPQLSPRSSYIRYVKDFSSSYLQQESERNYDEIISQNLSDFHQEKKLINMTSSRRISKKLETFKFTKIAKQNKIIEKFKNNLLQFSYIMPQKFKAKLLQIENYLQKDKVKKTSTQITRKNQKNIISPSNIFLFYWEIFNILIQFISLWICALIISFSNSKEINFQSFGCLFISINIVEIIISINKEIYIEGQIINTRNDILINYLSKSSHLDVCSIFIWIMISFRMIEQNTIIQVFAILLLIIIFARIFRVYDYIVEQLYQKGFGGYAFDLLTLVVSIYFFAHIMACLWLMVGFNSNGERMNWIRDNDLENESIWTQYNNAFYWATMTMVTVGYGDITPKNNYEMAFANVAMFFSSCVFAYSMNAIGILLKGFNDVKQNYKYIKCQYQKKVSHNNELIYEIQQCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBH5</accession>
    <name>A0A8S1NBH5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">PPRIM_AZ9-3.1.T0850227</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBH5"/>
    <dbReference type="OMA" id="DEHQYYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000632">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000392">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000557">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001751">
        <property type="entry name" value="S100/CaBP7/8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00303">
        <property type="entry name" value="S100_CABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="63"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="361"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="399"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="435"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="471"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="CAD8090357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="506" mass="58106" checksum="FD8372A054466E74" modified="2022-10-12" version="1">MGVCSSNPKRKYASTRGSQSTFYKTQEVSTIKSNKSLKKQRTNFKVAPNIFISLKSGSILSYYKVQSTLGEGTYGRVSLVIQKSTQILRAMKQIAKDKILVSQRDKMIQEVNILKNLDHPNIVNIYELYQDEHQYYLITEYLSGGELFQRVQQRNNFNEKVAANYMKQILSAVNYCHQRNIVHRDLKPENILFDSKTSDDCLKIIDFGTAKQLEQNSQLKQKIGTPYFIAPEVIDQNYNNKCDIWSCGVILYTLMSGKPPFNGNNLNDLYKNIKQGQVDFNGTEWKDVSEEAKSFILKMLTVDPTKRISAEFGLKDPWIVNNQKIEKINPRNLQNLESFHNKSKLSSAILQLISTQIMNSKEKKELIEGFKAIDKNGDGKLSKEELISCYMNLYQDEIKCSQIVDKIFRSADLDHSGTIDYTEFIIGYTEMQNLMAKEKLETAFKLFDKDGNGIITKQELKEILGGLDLEDKQWESVFLELDTNGDGEVSFQEFTQLLLKDTKQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCZ4</accession>
    <name>A0A8S1NCZ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0870047</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8090937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCZ4"/>
    <dbReference type="OMA" id="AMTSCIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000721">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF11">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="14"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8090937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="273" mass="32319" checksum="DB330A699489FEA0" modified="2022-10-12" version="1">MIIVLLILLFDVNADWYHSTLAFTLAANNYQQHLGKEKHVVLDFFTPWCIYCQHMAGEFNQVFEHYQATRPDILIAKMNCDEQQNQHICHHFGVHSFPTILYFPPGQDRPTSQFQNHRRYDFFVQWIDSLAQMSEETKQEIQQQKLEKMLKEQEEQEQRLREMMELERQKQIEFEEQEKKKEEEIRISQSIQQQIVEANTFIDTIKGMEKEIYALQQNTAELNSKLSQIKKMPKVNVNHALVFAIIGFFIGIAMTSCICLFQKTNHVNTEKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDT6</accession>
    <name>A0A8S1NDT6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0780117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDT6"/>
    <dbReference type="OMA" id="YFCDEKL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="175"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="232" mass="27413" checksum="8E890CB41BA110BE" modified="2022-10-12" version="1">MTCSLIICYFCDEKLHKDKENHITTTLTFTTQFPTQSNQNNLNQTIQQKKLELQELKDKEQKMAKIYQEKILHAQKKYEHQINQLEERLQLASQFMNQMQDQVEELDVDKMQNELEGLDKSLKMDIKKAEQEQSILLEKSKNADQLINKLKQATDIEQKQILKINEVLEGFKAYSEQLQKEKDLLMLDNEKLVGEVEIFAKFIAENGPLLEEISKVKNEQQQQQQQQQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGB6</accession>
    <name>A0A8S1NGB6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0830025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGB6"/>
    <dbReference type="OMA" id="MVEPTIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="741"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="580"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="750"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8089161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="797" mass="92938" checksum="1AC134768493BA62" modified="2022-10-12" version="1">MIQKFIQRISYFDTFGAEVKLNFRKKETHQSPFGGFCTILLVVVLAIVFVQGAISLIKQETFTISSNRILNIDPPTTMLNYNDYMMAFQVEDPIIDGTKQLIDFIYTQYEQSMNENGTLSKTEKYSLSLEKCTVNHFQDFNESTIYNKILKDQLNGYYCLPLNYTLTLEGTYKSNTFQYGRITALLCSTNQCYSTSEIQQFQLQGYFNNSFKINTLMLNRVSNLNLESNYISYIYSDFYISTRLGLETKTDIYLEKQAMTIENSVVPGIKDIKDEHVFSIVENKLTPNAVYTSNITKVASFYLRLSQSEMHHSKQYYRVDELISYVGGITKFLATVIGYFILRYNETGLQIKMANALYQFDMPEKKKGELVFSFQSLVTKIIDSMKSVDDVVQKFKHSAHRVIHMTRVATALRLPITSTQQYDKNEQDNVSVHTHRVQIINHDVHYGNLKSEKSLDQSHENNHQPNLDKDKEKFLDQFIKLILESKKKLSFGVHFIVKQVTGSFKRNSTTNYQARIFEKSRKMILTDMDILVVMNKLQEIEKFKHIFLNKTQRKVFNYLPKPVVCVSENRSEIRHHDDQLQENDLTNKKNDMLKQKSSSKSLLGGRQIYNTERKFKRLYKAYESLALNDHSSNEDLEHHYKSNKDYDHQFKYNNEELEHSFNHNNNLEQSLNKRLLKMVEPTIQYSFNSLVELEKISRQSKQNKSKNKRVITPALKNRMVQLDSQNEEGEDDNDMDCITLKGSKKEQQTKRSPNSLSNYNIKKTHNTLGSSSRQHQEIKLTKVQSKQDLIDIEVLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKC9</accession>
    <name>A0A8S1NKC9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0920109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKC9"/>
    <dbReference type="OMA" id="ECTENAI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="49"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="237"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="374"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8093078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="693" mass="83102" checksum="D3182520F360446C" modified="2022-10-12" version="1">MLTASPHMKSDKPYQQSPQYKKPHPYNPEASISLKNSINLKDFQFNEELEYYKNRSADLEQYARQLKNELDTTIIRMSKQGALDEKAELMIHQNQILSQDIDKLQKNFAQKKTECELWKSKYEQQLNSAVQLKAQYELDLRKLSNELKMLEERNSILEKERSHEVEATKTNFSIQNEQQRHSYLTQIDVLENQLRKLREYADIRDKEIYELETKLTKVLQDREYVETKLLKDNDLYRIKLQEQERDNQIEMNNLRQKLDILNQGQLDNIKNQYMAQAEILDDEIEKLKGLLGIKNEEIKTLIDQNERLRANYEKEIETITLQFHVLKEKMFENEQRFQEESQQMENNLRSQHEIGVDTLQQHHQNSSQILENQIVHLKGQVLEQTKQLEELQKIRQQLLQANLQDQENAQNIINNLKKEILEQQNRYQDQIQKNYQDYDLQKKELLNYNQQQLEFNQQLQQQIQNLNQIIELKEQQYETTLVQFKNMNDQLINKLNDLYRDNESIKTKFNVTVTEQEDQLNKQLKQIKQQEHKIQQLQSTFQREKQNAERQITNLQNQNRQKEQQLAELKAQLKAAEENLDKIDNELQETKNTLQSQIEEQKYNYEQEIEQIQAQQNEDLMSTKKLYEEKETSLNQELIKLKGTLQTQQTLNSELNQKLDEYGQQIQNVVNNSDVIKQYEILSEINLVNRLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTN5</accession>
    <name>A0A8S1NTN5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DBF4-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0930182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTN5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="33989" checksum="6088C9EFC30F19C1" modified="2022-10-12" version="1">MAGEKLQSSGKRKCQLKCPDHQFNLQPKSTKCWLKNITNIDFCPLYNDALSCYENIVPVFNQDEQQCLPGFILEVDRNDYIKSMQKYKEDHLKKVQEYTTKKTKLQIPKPGRKHRYCGVCRKPYVDYLDHIKSADHINSFNRHEFVHVILKIVNEDYKNKDENKIQNDICFDVATSAIPKKRGPKPKNQGQQIEPKKRGRKPTEPSISKRLKAQPIQEQYNPYRPQQTPYFPQHYIFPYPTQLNQMFAQLPYQQLVNIPFQIQLGQDLNIERKNEDMNGQIEEGPRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NVE8</accession>
    <name>A0A8S1NVE8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0930051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NVE8"/>
    <dbReference type="OMA" id="YEANTID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="280" mass="33644" checksum="E30392B47CC0ED4C" modified="2022-10-12" version="1">MIKYNSENHFKFHNVKVFDKERMILYFKRMPIEQEENDNLYNGLNQLIMKERIEQTKQKVEEGKEVTSSVKMDQGELPKNRTLIIMNFDSPFDRKFIAKVFKLCGIIRRVYVGSMKVVDNNKKKLLHVGLVVFKNEYDLTKCFDIEYFQQRINNKFKSAPISTNYEKKLLQNYEFVDEKKNQILEEAQKEGYQIAMNKYGDFETNVEKPKFEKRNWVGRNKNKTKKNVDVEEIEQVIQGLDDNLPHQKKKDILKLQKQQLQQQFQQDIENLRQKQKKVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYW5</accession>
    <name>A0A8S1NYW5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1020052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYW5"/>
    <dbReference type="OMA" id="QEFACKT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001034">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="86"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="464" mass="54068" checksum="72F2447C1176BFAD" modified="2022-10-12" version="1">MDLQNQRRKSKFSFSRAPICDNTQTSTLQQLKNKFKGQMLQLINKNDNGNNKEQEIQQFLSRISIELDGDDYKYIKFELSEYQEKYVEGEVLGEGCIGLVKSITRKIDGQEFACKTVKTDAEEIVKNMILEFKNLKKLSHPRIVKMEELYIQWNEGFQSTGTVYVVMEKVKGSEMFEVIQKQKNYSECIARILFKQILEAIDYMHENYCCHRDLKPNNILCAEDGRSIKITDFNVSKFTDGYKEFGNLNEHGKIEMWTYTGTVAFSAPEIFSGGLYNEQVDLWSAGVILYVMLSGELPFNSDYLNDLIDKIQQCKYEMVGPIWDQISYQAKDLISNLLQFDPQKRLTPQQALDHPWVKNVQNQSDLPRDRLKKNMARFLNWKPQETQVSEKKIKQLCFLFGAGEIWKRNSVKHSSVSTLSEEFKKFKSIDISSFKTTEHIQVVKDKNSNHVYHIDYPFSDDESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1H3</accession>
    <name>A0A8S1P1H3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1020015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1H3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="212" mass="25415" checksum="05B82D777CC1B3CA" modified="2022-10-12" version="1">MSANTEQALTMQLILLKLYKKDFNFQRDSLIISQNLTNVIEGQQIICIQISWPQLRDQRQQKKDEENQKFQRRRAFKIASHLAQVERKQILELQKQKFKINVIAAIFCITPSQVRQNLIHLKIIKQKEQLRITRFQQEWLLQQASFQSNQILFNAKSFLFAFKEQFLNQNVLISQFGKIYQHQIFDIEFLLPNLSSSLTKMEKEKRQRILYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P301</accession>
    <name>A0A8S1P301_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1040003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P301"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="25361" checksum="20B2BFFDA39FBC1A" modified="2022-10-12" version="1">MTGCQNYSVKLRIYTLRRKKQKLLKNLLKFQKQVQILLMLEYIEVSLVLNNKGNLSLDVNNISYAQFDFEEVLKQDKQNLKALKGLTITQKMQCNYELGLKYALKVIMHEPQSPLGNFNAADCLKFLGQYNEKTLQYINQAIDQEKRRYEQRIQIYFRNKGMVIFYKGEILFGLNRVNEAREFVLKALEINQNYELAIKTLKRFDEILQRRANT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P9U8</accession>
    <name>A0A8S1P9U8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1110041</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8099946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P9U8"/>
    <dbReference type="OMA" id="CISECFN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="332"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8099946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="575" mass="67511" checksum="FFDE32D3BCA7FB5B" modified="2022-10-12" version="1">MICQSQAFLPLNENQELEEQFYSYFPNGANWKTSQFFQDQTYEQFDSKVTTFFSIIGIQKNLANLNKDCQISQSNLFSVQFNKNDFSLSNMMEYLQSIASKLQISLSIIIMPKQIKQYGNIFHQQAKIIIQQTNNKKKLSFIFGLQSKMKLNQNPNFNMQPGNELNLEIQDLSQNNNIQSGHFFPNKNLNPFLSSQNIINQFKQQLSPPNINQNNNLIDQGSQQNQQFKMKCPQQNLLKNNQQIIFNNQQQHQQINPKQIKFINPQQQQQINPKQYRIDNIKLNVQQQPQKISKEIDESQTVLKTILIEQIETKQEKSNYNIFSKNEHQKSVTAICDICSNEYERISQQQIFLPCCQKPIHKKCFQEELQNLKEPLINIKCYFCHQNFQTNFIKENVDTNIFIMMVQNQLNLLCISECFNCKSKISILPEEKQKICKIECENCKTMICSQCMKNFHGFEKICQSVKQEVLKHLEGYQIIFCPFCDFIQTKDEGCNHVTCNKCKMDLCSVCSVDRRPILSHGNHFHRIGCSDHRYKEGQETMTKERIRGCQLCIQNPDKYCHIPIDLKTYKQEKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PA03</accession>
    <name>A0A8S1PA03_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1110078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8099996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PA03"/>
    <dbReference type="OMA" id="EVDIHEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000260">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24413">
        <property type="entry name" value="SPECKLE-TYPE POZ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="115"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="36815" checksum="14CDB4F46C4380F8" modified="2022-10-12" version="1">MLKKKGSEQLDIIFEAGEDLLNFSKNQVIMLKNNSIKLPIIRPRTFLSYSLEFCGITSLNYKMLRFQSLGRDIRPETSIMTSCLVKVIHTTEFSQMAQNPLKTSFQGLFIQGSHSDILLQINEESPIPLHKCILACRSPKFNGMFSSNMIESNQNLVKVEHKKPDLFKIMLQWIYCGYWKEFPEKIEDTCDLMLLADEYLITDLKQKCEEDIISKLDSSNILKILLFVEKHSNIISQPLLEKTNSMFIDDFDKIHKLNPNLEQDITRVPGLMTKLFQNLHQKKIRKGRKVHFVVDDFEESDSDDYVRNYTQHFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PA74</accession>
    <name>A0A8S1PA74_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1110080</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8100000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PA74"/>
    <dbReference type="OMA" id="DNQEIMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="40"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8100000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="402" mass="47154" checksum="063684BF43378224" modified="2022-10-12" version="1">MKKNNNKKTSNTKVKVNPCSNDRYYMDQSIDEEVYIKNNYKVVRELGRGGYGVVYKAVGFNQGQIDKNKKYAIKVNFSTVSPELIFAEIGFLKLIYGKENMPQLVNLFLIDQKIYIVIEYFTYKPFITFFANFDMMEIRRYLYELLKALNVLKLNGIYHRDVKPGNFLYNPKTGKGILIDYGLSEIDRSFVAKLIEKEKELSKKNPQSDEVQEIRRKINLYNEIQKTIDQIGNNKIGTESFMPLESILHYNDQSYEVDMWAVGVIFLQFLTKKYNLFSNVRMINKPVVTKNFFYVNFILELASLFGSEQVKQICDQFEYDLKLPTVVATKPVQWKSIININGFDNDAEDLLTRLLCLNPKQRIKVEDALKHPFFKPLLDQEKHTQTNFQDDQEIIQQKLQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBY7</accession>
    <name>A0A8S1PBY7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0560245</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1120004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PBY7"/>
    <dbReference type="OMA" id="KEVXFNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8100233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13820" checksum="7289A531F771B3D3" modified="2022-10-12" version="1">MYQPILQNQQNQELIIGAPEIPISIEYMAQQPQCQPLIQLKDRRKFLVLLMLTCFCQTGLELAISLISAYSINIQDLKLSFAIPIHQSKKFCQFFYIQQQLLHLYVFLEQDYFTIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PFB9</accession>
    <name>A0A8S1PFB9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1150094</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PFB9"/>
    <dbReference type="OMA" id="FTPFQHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8101403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="669" mass="79450" checksum="FB901F80FC28B102" modified="2022-10-12" version="1">MSNVTSSSRCPFKYPTEHLKNDNKSMTFRDFMQQQQIKEDQKFQSTGQLSTLQTSPIMSKKQRWEKQLSSLLRQEPQNQFIIKHGSQSQVTINEFGNKVKSFRSQEQSPTKTLYQLPMISDSQICDIVLPNTPVHFKFTPFQHKIRNPHSNSLRNVHEQLEQLRRPKVQQQHRELKQLPKTQTEIISKFFETKFQKIIKTQIKQYGSNELLDQHSESLYHRVIQDRQLKNKYFGKHLKYLKDIYKSILGIGYTQEILLDPFRMRSIHAPLFIKKEQFIRFKYLFINQYMEMETPVELLFKGCYKLENFKPLILNQRSDFEIFGGESGINEVAKNMYEKIFKDYTLSPYFKAIELEEQAIKFAKLFAQLIDHTESPNYTLEVLRERHVKYKLTHVQLANFKFYLSTALQKLGIGYKHLRILLRKMDTYKFAILNKPSLQECINNAPGGYREFIENFVRLCSSEPILYDLVQKRGKQRFTAHCENVFHYFFRDNVKSITDDDIESIHKNKTIISEKVYRKFKEKAMQAVGKVTNDPILLSDFEEDWEEITPILMNKPKKTTIKLLGGQAVINRIASKLENEIMQRPLLYKVFEDNESQVGPNLKCKLNLILYGLHFYKRTDIEVLHKRLRIREQAYFEFQQAMKVVMQDEPSKLQWVIDVIDDYKKHIVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PFY5</accession>
    <name>A0A8S1PFY5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1170122</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PFY5"/>
    <dbReference type="OMA" id="QIDSECG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="397" mass="46553" checksum="85EFA4EF3E926E72" modified="2022-10-12" version="1">MDSDFHQEIVDMINEIYEQIGKSRNKLLIQKLNERKEQIYASIPQSGSQILSNTQNREFITQYLDKFVKFVSDQKNLAEQGFFKIIRNSQTTKLQNIFSKKFTQLPQYFKSLECQDLSATGFINNQDVVHFKVPVNFTAISYLLDDEIDFIVCITLIFKDICDEEAFIHSLNTQKLLKKIPELNQFEFLKFLKNYLTITIDLPQQIIILEPLDPIIQFSPLLKSNNQNDNKQELYLSNDMEIIISPTYKIKVLNIFNKQNKPLNQNYISKNHINQYDDNLLIKNVENYIILQPLEQNINHQQIVICDLKKSILLNEFLRLPKEISVQLIHDGVGFLFEVLKEKPNFYTSLSFKSPIHAKTKHINICLIINNQLKLQIDSECGQLFFNSNDYNKNLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJ73</accession>
    <name>A0A8S1PJ73_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1190098</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJ73"/>
    <dbReference type="OMA" id="APIQICG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="123"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8102823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="321" mass="36557" checksum="66E46DD05C4BC72A" modified="2022-10-12" version="1">MAKDKQGEIDVDNIIERLLSVRGSKPGKNVNLSESEVRGLCVKARDIFISQPILLELEAPLKICGDVHGQYFDLLRLFEYGGYPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYTIKLWKTFTDCFNCLPVAALIDEKILCMHGGLSPELSNLEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWADNERGVSYVFSQEIVQVFLKKHELDLICRAHQVVEDGYEFFSKRQLVTLFSAPNYCGEFDNAGSMMTIDESLMCSFQILKPAEQGAQGASQQNKPPSAKFVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PP31</accession>
    <name>A0A8S1PP31_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1250066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PP31"/>
    <dbReference type="OMA" id="SSEYDLW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="84" mass="9923" checksum="9998DB2FED8F758C" modified="2022-10-12" version="1">MDSFNLLNNKFMQQAMERKSLRSINLSQKNLDTQSKSIEYDLWDRNDGLDSPMTPKIQIPNQFYPKLINNVRLSNKRLSCMDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRF7</accession>
    <name>A0A8S1PRF7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1260064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRF7"/>
    <dbReference type="OMA" id="LTEGMMH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="305" mass="35456" checksum="80E0039BFD5CC12F" modified="2022-10-12" version="1">MNRSRRKELLQLVYKKRSFLSPSIGKSIPNKPFRRQSCRCSECGGVGPFQERQNAQHPQTYIRAHHCKKRKRKMQRRKGTYLTSTSLNVGQSENNTIIIQNAPKLRKAKTISITKELQQTIRKGKRLLTKDDQQIKYEFFYNNELKLSKGFLESSLNSQTYEAALISFVNFTKSPSFVLNSYNNIINESKTSSDEEQNQKHLQDKQLQITTPKIKIKNNSMHSRKLTEGMMHSNVNKTVQQFVQSCTTIRVMNSPKPSKHIKLPSINSKREQNKFSVYLLNSPRRAVMELKSQITSQKKIKVFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PS91</accession>
    <name>A0A8S1PS91_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1270103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PS91"/>
    <dbReference type="OMA" id="VITPSCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="143" mass="15835" checksum="A073528EA2D2D1A5" modified="2022-10-12" version="1">MGSSCISNQKDTAFEDLFSTQITSQERGIQNINQTIEGNFQQVKLLKESLEEGIQISNIQTIVITPRCELEGAKIVGELVKLGNQITIISKKIPESPHVSERSISKKGILKNKQDINVTQSADGCLLYPSPYRKSAKNIKFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUG9</accession>
    <name>A0A8S1PUG9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1320079</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-48-linked is involved in protein degradation via the proteasome. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8106967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PUG9"/>
    <dbReference type="OMA" id="CIWYCVY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000158">
        <property type="entry name" value="Polyubiquitin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000014">
        <property type="entry name" value="Ubiquitin-60S ribosomal L40 fusion"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="153"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="229"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="305"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="381"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="457"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="533"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="609"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="685"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="761"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8106967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="837" mass="93379" checksum="2F70A87B119372CD" modified="2022-10-12" version="1">MQIFVKTLTGKTITLDVEPSDTIDAVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDAVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDAVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDAVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDAVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDAVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDAVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDAVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDAVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDAVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDAVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PVU5</accession>
    <name>A0A8S1PVU5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1330049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PVU5"/>
    <dbReference type="OMA" id="NLIHKNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="113" mass="13330" checksum="B4884983E149249A" modified="2022-10-12" version="1">MFQDSKRHGPSLELQNLGPLHKSISKITDPDIQSFECQKKTYIHSPRVQRFDKLGNLIHKNQKKKYQITFRDEVTAKSKLYDIILVDNWKKYNIIDEEKEKVGCCQSKSCQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZX9</accession>
    <name>A0A8S1PZX9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1370161</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZX9"/>
    <dbReference type="OMA" id="YTKERDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="100"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="391"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8108642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="60105" checksum="17FF1E3640BCD8B2" modified="2022-10-12" version="1">MGTCQGTKKTQQKKPNLEIQIPNNDNLENSRKSQNYQPKSAIVEKKTPASSQRQSLRSQQFQFNSASSDQALKSRFSTPMVDLKNLEQDQVRTCKHKTTFKMNATVKKVAFSKFYSVQQTKKNSFSKIPGGSILVQHNMTGKLHYADILENTKVTKFYVDELLRCQLQHPNITNVIEILEDKSQYIVIKECAQGVPISYLESPEKQASILKQIIEIVCYLHSKNLIHGALWLGAFERNNDLVKLVDIQSIFIRHEPTNHDAEYVAPECTLDSTIYTKERDIWAVGMIAHNLICGRYFKATNAQDIIKEIEQVRIKKHLHLDNSQIKEFLEKFLHITPEKRITLQEALKDQFLPKTYEMEEFDQILKRNQKLQKISVLQQCLLLLFINQFEEQKIKYQKVFQNLDVDYDGKISKNECLALYPDKEEQINSLFNLLSLKDKMDFQEFLVISVDRTQLLTSQNIETIFNLYATVKKFLLLQTLVNLYKSRENELQEAFSEYDHAHINFKTFETLLTDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1Y3</accession>
    <name>A0A8S1Q1Y3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1380098</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="9">
        <location>
            <begin position="8"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="230"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="271"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="779"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="1261"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8108869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1444" mass="170100" checksum="02E670CF8F34FF87" modified="2022-10-12" version="1">MQNKQNSCAVCSLKTTFGCSQCKSVFYCSVEHQRQHWTIHQSSCQSLKNQDNKQIDDTISKSPASKPQKFEIQQTAQPKSDKKLIMNERDILIEDHDRYRQISIRLLSQKDYQDAIQQIKKTIEFASKLELIGGKEDTYEKIADQMLLVRAQIKLLDLETARKTLIQIFEQVKLLTQTSLKNDQVKVSNILKVMAQLFYETGDQRQCELAYVEYTLMIEKCYGEDSNSTGNAYFLVGTFYLQHKHYTQALACFKRALNIRSNINPNLESVSDCWYNMGVIYKQQNKKDQAIDFLEKALKLRRQIIGQNSLQAAVCLETLGKLHIQYNEYQNAYSRLQECFNIRKQLLKNPQHQDIERVSKLIQILYQKLQDQNYNKQNSTLQATILENNFNRVLEMEESEKQEKSNSSPSRKRSQLKTDQKLSQLLNISSNLLESFSIDQLIQLKLLQQELVKYKNRQEQQQIIIDSQFMNTLSSIQYNLLREQNPQVFQLLQINKPDEQQSEYESESKPSIDQKMYASNQAFIKDCPTPRKLSEIIKLKFIIIKLMQKKEVIQERRPLLFIQLNNDYCQLQIIYITMSLQPRLSPKNYKKYSIFNDSINIRTSRNTSFDYEFQKRRNSNIRFKNSGSVLRGSKASDFMGNLIAHFQYGQNAFREKNLIEAIKYYNECILIDSLHIPSRYMLGVCHFSQNQYEKCIKELKFVKEQQPNYNKNVYIILALGYKKLMQLDWSIRTLDECLQFYPQCYDALIFKGKLELKLRRFQDCESTFKSAIKINSKRSTGYHYLGDCQRLQNNLDQAIKNYQIALQFDTSDKSKLNILKMSICLYEMANYNQALQMIDQYLSQDEQSSEANKLKGQILYKLGEKQESQLYYEQAIQNNNSRNAVSKAMIEISKFKIESKDFYSLYHTLLRVEFLDVDKQQLQPYIQFSEAVVCLMKKNYIQADEAFKNLQQLDIPIVIQEIYHSYIGYLNMMLNNFEQALKDFRKNKSLPYNQIICEGIINFNSFEIAYSKFDQALSYNQIDPNMYKAILLIKEGLKQENSDYVNNSLQCLIRAQQYNQNNSQLMYLKSLAYMLMDNVEDAYKEILTTIEKADENLADHYYLKGLILALNGDILNAVQDFSVCLGIDDQYQKAYLQRSKCYSYLGEMQSAFDDMNSYIQFVDDFLSAGNLLYNSKMYEEAYKTYKQEPNLTIEHKQQISICLFQLEQFDGEEFKEFIRDKPFDQNMILAYQQILKYEYDSAILTLQKKVDSQSSFFFNKQYLHQYKGICYLYLQKYQEAIQEFERCIQQTKSDTLIYITNYNIMFCWMMLKKFKYSHTQLEQLIKISEQKHVLLTIRNLLIDQTEFTLSDNPQGIEIMLADTIKLQPFISTQYTIYPYFSLPIAKPKDITPQFDFKIIESLNPQSIECRPEAPWIRRNENGVLFTDNLQQCELSQTENSSPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QAP7</accession>
    <name>A0A8S1QAP7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1490066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QAP7"/>
    <dbReference type="OMA" id="FTMEQTW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="191" mass="22752" checksum="FE66FCBC086EB404" modified="2022-10-12" version="1">MKEHQGLMTIEFKPIKQKYFLHEKEQLKQDPFVESLQEYFQIFRRRIILHLLLCILKSQQLIAFFFLALMIPSIILQADYIELDVSEKGGIFTMEQTWDPPISIQMFSKTDILLLDVQLSLNGKEIEEFTIYERLYMNTQYNLTSNTNYSFTVRLMAQFDFVPTSYLIIIVVMLVISLLYSILTYTRYKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCN5</accession>
    <name>A0A8S1QCN5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1530011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCN5"/>
    <dbReference type="OMA" id="KFKGQIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="308" mass="36026" checksum="707DD3CB0544530B" modified="2022-10-12" version="1">MFTSALSQMLEMKNKDYDAKLIKQNIMSMNKLNQIEIMALMEIVYQLRLCSQKDQNKEELLNELQYFLQGQLSTDRTAIANTNNFLEYKTKCNATNVGIMILNHQGQFILSDQISRNILELNSKEELEQKNFFSLISKVSEVKLKQILKNNCLLQNGQVKECFNISIYSDRNRKKSLKYLNQMAQVPLKKKIKKNMTENVKILQKEQILMAKYLKSIQVTILKVTVQIHKDFIDSFLDSDDIILSNLDTLAQTTVNQLAICEVKELDEPIRLTVQELLQDPYISKHEIKWNKRVRKFKGQISDDEYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCR4</accession>
    <name>A0A8S1QCR4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cilia- and flagella-associated protein 61 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1530031</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038884">
        <property type="entry name" value="CFAP61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056299">
        <property type="entry name" value="CFAP61_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032151">
        <property type="entry name" value="CFAP61_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21178">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21178:SF8">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23150">
        <property type="entry name" value="CFAP61_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16092">
        <property type="entry name" value="CFAP61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cilia- and flagella-associated protein 61 N-terminal" evidence="3">
        <location>
            <begin position="12"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP61 dimerisation" evidence="4">
        <location>
            <begin position="1426"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="456"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="648"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1254"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="460"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8112864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1626" mass="191173" checksum="1460AF2DFAA6591E" modified="2022-10-12" version="1">MSFFEQQGQELQIRKADLDDYDEIVQLMQEEGEEDLQQLYAYPKILTLFERSYLSVTVLDSQNNIIGNAVFDDCPQGVTGQVDFKHENLWEQWIHDGWNIDFHVSSFNCLWMTFFFLAKKRYQLTEEQQLQITRQIFQKVYDYLNVVNGIFFLRRNEAKDATQQELDIALENLFELLPKRQDFQLKFMQGVNQNCEIYYSPSSMVIDLLEIRMAREEDHDDLAAIFNRQSDVHTEEFGEFFIADLIATQNQTRRQARNYRSDGKAIVGQVGDKAVGLMSISSDIDYRLLGQCFDLEVFDNLYKAEYMEAIRNRLDQLALEEQINKQIITYKKQIELTKEAMKCHIVGQRLYLQQYCFDRDQEIKQKIDDYGQEDLAKTLTPQVVTDMINGWLKDYQVFKPSDLFLEYPNLFKDLECITIKPIQILLEALEFFGLPKGYMNGEGHWKDWAKKKEEEQKALSLKRPKRQQKKTNKKAKKEDKDEDIFKPPPYFDLGPFLQAFTKFTSVSAETRTQFRKQIEMRVKELIMEFCTENGEMDPSRHVDLIEFPEILRNKGFDIGAPMGENLAFILECFGELEVDNRIVQKIKEEKDAKKKDTNLKKVGDDAPKPVDVLQKMTSYQEFIGAIQKLKKYDQMMCRLQLVRSNTIQSEVEEIIQEEQNQIAKLEAAKQIQRTSTEYDEYVSQLQSIDQLPEVPKTAQNAVVINLFCIDERFESRSLDFVERAFELFPDREYLVLTQPYTVQETTLLQHFLQVPRKKHSTFEHVLYIYHRNSLDSHLIGLRKLNQNEWAQCQFLVENLLDKDQIERDVRNVNDNETFIVYCKEEIIGLYCMRKYVNLQYLKSHFCIQDHILIAEHPKHLHTRLLHGILNPLFAKQTRFILREICRLMDKTCIMLEIHDRTLLPDIFQEFNFVRSRAFAHFLKQKWDWTLDQDERERMGAILDDRDPYDQNQAPFSLAMLTKKQLSSVKVSNNSRIVVVGASDTGLSFIESLLTIKDINFTNIILLAPGGLLTMHVKHEFEMLKAMSTNYTLEELRALMLDARVQVVDAKMVKLDKKGNRIKIDKNAFIPFDYLIITVGLIDTELQSREKVSFGLSKSPYYKTAQFINGLYSIDDPYLYQHFKRTGFKNSNIDLLTRKKKPQNITIYGNTLSTITFMNGLLNRGVHPSRIHYVMPPKTFQKQTKFENNKQRLEQEDKIIFDPDQFENDEVKNKVFDIMVKMGINIHYGFTLFEMKVGKEGFGLNSEDVLQQVIFRKQADNYEELKIEIQRKEQELQELKDNSENNMSKDMYGEQEEGENQLEVLAREIEQLKASEYDYLELESRFFITSGLIDIDKEIFNIIHENGLVYNGRLIVKSNFQTTQENIFACGKICEFSQRYKHHSVGKSLRLDKYNGRELGQKLSKCILEQLNLSYLTSQTYSVDELPQLYMPIGQGGIVPHKLYYYHIKKNDFSKPKHLQQLPSKPILSDNFNNGNGHFLQFDIDPNGLIESVTYFGSEAVHIPSLLQFIELSVKYLNKLEQRGKLINNVSEFLSENWAIALYHEWFSEFRHITKSEMLKNELIDQVLEKAQEYARDGRYMDENFFEEIKKLITRDIIENIQEGTIEFIRENQNHLPMYFIPKKKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFI4</accession>
    <name>A0A8S1QFI4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1570110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000162">
        <property type="protein sequence ID" value="CAD8114026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFI4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8114026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="88" mass="10898" checksum="A81EFA2D425D24CE" modified="2022-10-12" version="1">MQSGENVANQVKSKEHNKRQKRIRKNQKYYIRLYLKSFHFLFDLLILKVLRMQLQRSFQIHHNRRCQGRQSMQLNILIQLYLLLMLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QGF8</accession>
    <name>A0A8S1QGF8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1550073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QGF8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="415" mass="50131" checksum="E9940476BD1E362B" modified="2022-10-12" version="1">MTTKEEFEKYQESLEGDSNVQDDQSYSQNKSVQTYLKPEVVGPSVLIEKAQTSYEFNDERYDLYSESREIFSKPNDSNSQYQDQEQKSEELYESNFVYQDKKYESLELQQLNNYQYQNQLSNGTIERQKKLSENDHSLILVDKLDIIQNKDRLNESVDMLLTIILQVKEKFQEEIKVQEKKDYYMNKLQKFSQKKIMKKFKIKQFKDNQYNFISQYLIRQENEESLKILLSCFIEYTFQWINLLDFKQEGKSQTFKEIQEFLKQEIIEQTTIKLGIQNYFQNKTYQKFDFIEFVINLLKIYQELKNLQTQELIIYQQQQEIYLQQLDAYQQQLFDFLKKISEMKLNRYKQKMNINFSLKTFKKLISCDAKPEVLNAIKAAKRKPKQFLNELILNDEEIKDFQEAIEKLLKKAKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKN4</accession>
    <name>A0A8S1QKN4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1740015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000183">
        <property type="protein sequence ID" value="CAD8116538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKN4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="203"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="425" mass="50598" checksum="AE1DC255D4DF9784" modified="2022-10-12" version="1">MNQERKHHFQDKHIEILWRITQDNQIQNQQIQFEYVQAQYNEDLGFGSGFGGIVKMSNGQEYLAFPDEQGKVNIFVDNQWVPLEKNLAEFQQYIPDQIQQQIPQYNQLNNEPSLYVEVRDQNNFNKYRGHITVSDYVSQTQSLGEELEDLYLEIEKNIQSIFDKNSSVYLNMTHKDPQLEFKLKMAKGKKLIDFFIPIKLTLVEQNQEERENQKIKLNKQNKRLENIEQNVNQLQQNLTDHSQRIGFISQELGNQKTELSNKIQNVELSFQYQYQKFLQQKQNQFKKFSINQMKKVKEYIYIIQKLKGQCEFELLLVDDEIEFQVGIIEDDDLDNIEKKNKSYFVVLPDGKFTRGTREFGKFFTHQLQFEDKLGLRVDTFQHTFQLVINDYPFPPVKINKSIQEYSFIIKSSEKLNRFAEDTNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQ58</accession>
    <name>A0A8S1QQ58_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2030009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000212">
        <property type="protein sequence ID" value="CAD8117551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQ58"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="171" mass="20048" checksum="A6ACBB6178DBFEB0" modified="2022-10-12" version="1">MQSAFREYFAYRTQMKGEIELNLASDIRIKAWLNCFDCPDTIRAVAQFWNEFIEPKVNDHQFLLKAVSKMADSLYLMFKNTEFKFSAGDPTGRIDHDMVLLKKRQQLIADAILDMKTNLGIGNQQQKKQQTVISLEPLNIEEMMDLENDQDLQAIERFLNKKKPKKQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QTT7</accession>
    <name>A0A8S1QTT7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2200015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000229">
        <property type="protein sequence ID" value="CAD8118037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QTT7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="48" mass="5716" checksum="D9A59CE7D3272308" modified="2022-10-12" version="1">MKGSFSKKEALLTSLIKQKMQIYKSLGLNQKYFERKKFKIALDIYQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JM14</accession>
    <name>A0A8S1JM14_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0040396</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JM14"/>
    <dbReference type="OMA" id="AQRSQFR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="688"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="696"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="772" mass="90894" checksum="F59111400C1A5AC8" modified="2022-10-12" version="1">MGQTNSQNLQQSKMDVPKISQLQFWKQSTDIRYGEIRIFKTQDQHAVAVKDHIFQDDEQWNQFKIIQEKYMGNKQSTPFLIQMIDLQHVTEKELCTQLTQAHSVYEYFDEYLERVINELSETKQYFDEIEIIAMLHCTLMALQKLNDQQKQHGDIRPLTISVTSYAQRSQFRQNHPFSVFKLTDIQEMTDMNAYRRCIAKQQGQYNLSPEQLSSLKQKVLRPNIDQNKSDVFCIGLTALQMATLSFVQDIYDIQSYTFNMEKLDDYLFFLKSQYSPDLYDIVESLLIMAPDNRPNPKQAHELLYKYRFQLEDYFRSSTIAEYIPSYSIDFREQSDVYQKQRVVGLQQHTLQNQDPNNQLYHQVENLDQRAKIALKRSQDAQIRFQRSPNQKFKVPIPNWEPTKSLQQYSSLQPITLQEILPLQNKQMNIQSDQIIIQDPPSNRQSFFKNLTNPQQYQQQNYNEHEQIIHNDDEYAYNHQEIDEQPKQSLVEQVELTNSNLKTLQQKPIVNQYDNNLFQQQSLFQSINTDQKKFPQQQQQQLQQQTYSQIQQQQSYQYSNQIQSKQLQNIEQQSQARSSTYNYPSNQINESINDHQRSPYALQQRQSLALSQDKVITQQLTGQQVEQTLQNLQNRQSIRQDSFGVGSHRPQQPLVVPKSPEIEPKNIFPQFEQEKQPQIQRSPIQPLERIDDDDQEPNYNPHLTESNDSQQAISVQSKPQSMRGTMVGSQSQLKVPPSVASRQQRPTVQKQSTTSQKNIYNLSQKKSSKIIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMD8</accession>
    <name>A0A8S1JMD8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMD8"/>
    <dbReference type="OMA" id="FEMSMDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="427"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="444" mass="51992" checksum="942ECFBC72228BA4" modified="2022-10-12" version="1">MLNLLQINQQFQTKLQSPIKKQKIEQIVQKIKEENIIIENQCVSQLMTCLNQLQLILSLQTNQFIKLIIRIGSVIKFDSNISIFEDILENNCDKELFIDKLQYLEHLEVKILSNGNKQLILNPNYGIIEKLTTPFHTPNLDELLAQFLRSQDLQIQLLIKLYCIISILQSQFSEIITNLINLAIQNQDKDEPIKNRVFMTNLDLIFQTQKQEKVQMEVKPINYQYQYNKLLIYQQAENNLITKAFDLLDNTMYPKRKRSFEKDKESAYQNFSQKNQFEMSMDIFRVDPYMSYQALFSQVAPPQQSPVLTHVSTFPKPNSLCLSPTNSDSHSFTSQKNKGLNYQFNSVYQHLIDQNQYAQISQQNIDAQIRQRKMLINQQQNQRVSRSKSNSTVIMPDIFKQICLDSGKSDEKNDEKSKQKGTKKTLNINQNQKKAFQNKQLINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMR8</accession>
    <name>A0A8S1JMR8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMR8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="60" mass="6766" checksum="44C3CB7DE5306C49" modified="2022-10-12" version="1">MIQIQGSDTQQLIIELVQINQLLIISGDTKMLDLLLIFQKQKTNIAFLIQQELQGIIGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN94</accession>
    <name>A0A8S1JN94_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">proton-translocating NAD(P)(+) transhydrogenase</fullName>
            <ecNumber evidence="2">7.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0040388</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>NAD(+) + NADPH + H(+)(in) = NADH + NADP(+) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:47992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="7.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="DEINDDX"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008750">
        <property type="term" value="F:proton-translocating NAD(P)+ transhydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05304">
        <property type="entry name" value="Rubrum_tdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000028">
        <property type="entry name" value="NAD(P) transhydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007698">
        <property type="entry name" value="AlaDH/PNT_NAD(H)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026255">
        <property type="entry name" value="NADP_transhyd_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024605">
        <property type="entry name" value="NADP_transhyd_a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034300">
        <property type="entry name" value="PNTB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00561">
        <property type="entry name" value="pntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006942">
        <property type="entry name" value="PRK09424.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10160">
        <property type="entry name" value="NAD(P) TRANSHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10160:SF19">
        <property type="entry name" value="PROTON-TRANSLOCATING NAD(P)(+) TRANSHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01262">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02233">
        <property type="entry name" value="PNTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12769">
        <property type="entry name" value="PNTB_4TM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01002">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="567"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="593"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="623"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="647"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="675"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="715"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="746"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="774"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="952"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1009"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1051"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1077"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1107"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1131"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1159"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1199"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1230"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1314"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal" evidence="15">
        <location>
            <begin position="36"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding" evidence="14">
        <location>
            <begin position="177"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8043165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1454" mass="154604" checksum="3E3A1DE85DC76792" modified="2022-10-12" version="1" fragment="single">MLSFHRQATRQLLLSARQCFSSKGDKKPSHPKFVFGVPKETFPNENRVAATPESIKNLVKDGHQVLIETGAGLKANFADNVYQEVGAKVVDSNSVYDQSDVILKIRPPDNTKALKENQTLTSFIYPAQNKELLNQLQEKKITTFAMECVPRITRAQTYDALSSMANIAGYKAVLLAANEFGRFFAGQMTAAGKVPPAKVLVIGAGVAGLSAIVTAKNMGAIVRAFDTRLATKEQVKSCGAEFLEVKINGEVVDGAGIGGYAKEMGKEYLEAELALFLKQAKEVDIIITTALIPGRPAPKLLSKELVENMKQGSIIVDLAAETGGNCELCKPGELYNHQGKVKIIGYTDLPSRLPTQSSTLYANNIGKLMQYIVGTIKDPNGKMTQVDLNDDVVRQSLVTLNGELKWPPPPIITSKSELQQADTSKAASHKKQEVKVDPYKQAWKNVINTSASLMAMMALGYQSPNMEFLKMLNTFALAGIVGYQVVWGVTPALHSPLMSVTNAVSGIIIVGGMELMDGKYLPGSFTGLLAAISVAIASLNISGGFLVTQRMLNMFRRPTDPAEYNYLMALAGATGVLGYYGGLYYGIPKEPLTNLSYLASSIACILALAGLSQQQTARIGNSFGCLGVGLGVAATLGYKGYSPELLAQWASMVMLGATIGGTVATRVAITDLPQLVACFHSFVGLAAVLTSIGNYLHVFPHLAEDPAALVHKLSIFFGIFTGGITFTGSLIAFAKLQNLLPSNPTVIPYHNQVNIALAALSVASLLIYNQTGSFAVGMTALLTATAAXLVENMKQGSIIVDLAAETGGNCELCKPGELYNHQGKVKIIGYTDLPSRLPTQSSTLYANNIGKLMQYIVGTIKDPNGKMTQVDLNDDVVRQSLVTLNGELKWPPPPIITSKSELQQADTSKAASHKKQEVKVDPYKQAWKNVINTSASLMAMMALGYQSPNMEFLKMLNTFALAGIVGYQVVWGVTPALHSPLMSVTNAVSGIIIVGGMELMDGKYLPGSFTGLLAAISVAIASLNISGGFLVTQRMLNMFRRPTDPAEYNYLMALAGATGVLGYYGGLYYGIPKEPLTNLSYLASSIACILALAGLSQQQTARIGNSFGCLGVGLGVAATLGYKGYSPELLAQWASMVMLGATIGGTVATRVAITDLPQLVACFHSFVGLAAVLTSIGNYLHVFPHLAEDPAALVHKLSIFFGIFTGGITFTGSLIAFAKLQNLLPSNPTVIPYHNQVNIALAALSVASLLIYNQTGSFAVGMTALLTATAASKVLGVTLTNAIGGADMPVVITVLNSYSGWALCAEGFMLDNPLLTIVGALVGSSGAILSYIMCKAMNRNLTSVIFGGFDVNPAAIQSTKVEGTHTEINVEQAVEMMVGSKSIIIVPGYGLAVAKAQYPVAEMCKTLIDQGIKVRFGIHPVAGRMPGQLNVLLAEAGIPYDIVFEMDEINDDXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNQ1</accession>
    <name>A0A8S1JNQ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0050285</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="TEQYRIL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035796826" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="17"/>
            <end position="1685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1246"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1338"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1445"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1501"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1527"/>
            <end position="1546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1558"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1588"/>
            <end position="1609"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="162"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="639"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="752"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="799"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="847"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="888"/>
            <end position="939"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8043631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1685" mass="195976" checksum="EB0091A05DA3BB9F" modified="2022-10-12" version="1">MEHLFILMNMLLIVQSNFNGVNLETSQEKLSYYLDTSTSAQNLTCQSLTKVYQSTLTGIPRVMEFKINTAYGYNQLLYTFELHFLSTQKSKISFYESGKKIYELEEDVITYYPAYLCTFLTQRAFIQFTSTTLMNEQQATFLVTTNSETKYWIKNLNIYAERCQKYCKICNDSLLCQECMPGFVLTFQGVCQCQYQFLTYFQGQCVYQCPVNYFFDGYTCQQYNQIINLLYDDTASKFTMKPDYNTTQRVSQTILDGKKVAGLFIQNEVVEYNINYPTGTIFNILMQVEFYILNFPSNLQQHLFIKLNNYLMSEYLILLNNNEMKFQVVGALKQSQCNIIGYTKCLKFTLIEKFENISDLSLISFQINLPLEIQDISWAISYINVNAITVSPITCPLKRFNNECLDKCPINSRTSGNECISILNDYKFSEILKIGYTDKLAIKQKIQFTNPCDFQLEIPLSVCNFYQNQYILGGEMIWKNDQVKLVITSNQPHYKVKLFFKAILIDPINIDQSLIVSIDQTKYTLNMNSPNSYCFSSQTIPGCIIQEDLGQTKADYLINFEQEFEHSNTQIEILIYCNMKLPSNSYCALYDLLILQANCPPNCKFCSSDSICFQSDSNIQVFYNCPNEGYYYSEGTCLKCLNSCKTCINQSSCTSCKDKYLLYGNLCICKMNLIESKKTDCMDDCHQSCSICQNQVNQNLNKVLKTIQLCASCDYNEHKVLNINICECFLGYYMDKSSNPNTCQLCNTTCKTCSNAMACLTCFPDQNRFYNNFQCQCIDGYFENGFNATCIKCNKFCKSCLYKEDYCTKCYTEQYRILSKENTCVCQNGYYDNKQDVCLKCNNNCNSCSDYYICTSCNEFQFRELDSHIKQCICQEGYYDNNELSCLPCNYTCSKCNNSNLISQCTVCPKTRQKSVNNLDVFECKCRKGYFDDGYLECQSCINLINPPITHYCYSQCGDQIIQWNEECDDGNLDPRDGCHQCSIQNNNCIDNICLKCQYHQCLQCIDGYYLNNDFECAQCSDQCQTCESQQNNCTQCKFYNPNTQQCLICSLDYGFIHIDNQCLSVCGDGIRTYQELCDDGNQLNGDGCNQFCIVEEDYICDIQCQKINYLQILLQEDKFDNIYDSKRTVKLKFNQEIKISTNNSITNFVKVTSITPNLIINLLSISDNTQIIDDYFYIDIDLNLELDSSSKSPLVKVIIQNHTLFTNQQGQSFKINSATIPLIDFIKQDQFIIQNTQNLAQLSSYFLYILLGLAILALIFGGLEIFWNLLDTLQLICYLKYFNVIYPYNLQYYLNIFGFAEFDLIKSYFDIEYIITQYVDTTQSDPKFYQEGYSTVFIVNIISVIIVFVTTTTTFIIIKVGLYFLHKITKDFSEDMILMEREQINIFTFLFYKLTNSCQKYFLKMICEFKSALIRTFMASAYDLNLAIFLQLKDLNFKHPILRLSSFSAIIVFLLEIYFIYKCFSLMNKDLATLKLKKSEQNYGSLYEGLKLEKNHIIQYFNLFLIIKKTIFMALLVFLYNTPCLQISIVSILSLFQALLLLYNQSLRDKNELIKQITCELILWLSIILILIFGFNEQSTILDYQQITNIGWTIMGFLSLIILIQLMIDCKQHFQFLDQKYQLLKRLRVWYNYYFQNHENPYSLNTEYSQYSTIFSKKAKQQSNVSKNQSNQVNQRRIISFKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPM2</accession>
    <name>A0A8S1JPM2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0050351</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPM2"/>
    <dbReference type="OMA" id="QDYWFFP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="4">
        <location>
            <begin position="311"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="161"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="228"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="256"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="279"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8043697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="545" mass="64642" checksum="9ED2985A99D8AB3A" modified="2022-10-12" version="1">MNQRRDKKKIASQQQQQQQQQQQQETLLKLKLKEKFGQNIFYIQPSNYSIEMKQFNFRNPTYGVTVEYKFLAKIQECSYLESSQLQFFMNLFRKAYSNVKLASATCYFFTDFVLTLQKPQESEQQPQIIKIDWKRPNVEVKSQEQNKIEDPLNQIIQKEISSQQNNQQKSNQDSLETKNLSLNNNDKSQLTQVQKQEKKQEINNNKVEQQPPQSQIIPNNSNNQLKQSLQEEQNQLDQNKKQDNNNIDIPKKNNLSSLKFQLTQQNQKNDQAKTDQFKKNSQQDSALQKQEINPTTNNIKQNKEKNQKQDVNLNQQKIDKALINENKVQDKIVQKQIDPIVQEKKKVPFEQVPVKQVQVETQIQQSQPEKKYHFQIINITQEVTPPQEIIQKSQLGQLMKQFAENNNLQVDQLMDQDYWFFPVNILQSHWISVLVQFNPKQIYYFDSFYEKIDPLIIKGIHSILEHYNVDPQNFQIKPIYIQQTNGYDCGIFLLLSLLYTLKNQEYKYNQRLVDKFRRKILYNLAVIGAQIDINKESFEYLIALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPP4</accession>
    <name>A0A8S1JPP4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050381</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPP4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="117" mass="13316" checksum="BCC0BCA7DA9989EF" modified="2022-10-12" version="1">MNKLISTVVKSMQSQAPLYAYHFPNGSVYNNPAVTAKRIIKVVGERLRKIDPERWESTPITFNTNWNDAAGYVDVATCIHIHDALEKEFGIEIKDRAFLVSSIETAFYIVNIHHDSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPY2</accession>
    <name>A0A8S1JPY2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPY2"/>
    <dbReference type="OMA" id="HKTLDVQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="433" mass="50170" checksum="F2531DB5B1E60DA0" modified="2022-10-12" version="1">MSITVEDLAANIETVLASLGDEKMVNWEVVEQTEHNLMKHFDQLFERLYTLKDSLQRVFLINIKNLRQYDAKDLVQKVKDKKLEPVVLKSLTQYAFKNDKDELLLNPSLIKGQVNSIVLPWVGNNLSVTDKIKETNEWLDQKLAELEVFKPKESQETPKIQDLQPKNEGNDKNNYTFKSDKLNEIASQIQTMHIPNTNNMISIKTYTQIVEGSVELAEPEFIRITLENRKARREVLYSDGAKYVQLLLDYVNDIENLLMKAQEEICKKIDISQQTLEQSEMLLMERGLGQHVFMLQASARQRIKDKLPKQKQVQMNATKEIIRYQIKLLNEKQDFLKNMIDKLPTTYESGQLVPMVLNLVMGDMIFEEHSYEEEDYISNLENPQLFQDPDMMELLKSIETGVVQLLGKTAFAQPQGGMMGNIPPQMLQKQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTR0</accession>
    <name>A0A8S1JTR0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTR0"/>
    <dbReference type="OMA" id="IEHQRNP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="311" mass="36100" checksum="BB915B8FE6561085" modified="2022-10-12" version="1">MNTQDEILLLKKCICEFLNKLLPTIKDPLILTRQRLSQFKTVLLDELKTIQDVYYYVQTLQFQDGLALRTDTKIEHQRNPSGTNQDYMNLNQNPTIPERRTNPTSIGLNRGQDPQQTNAGNTINIKINNTTALNQQNQIVEDQLEIPKLGKDIFFKRPSTRKLSIKTKDAKVQENNYKPIDEIQKVNVYGQKADTFSVFNNYSKKTPFFDKETLLAEIKESEKIIRKNTTTLNAVYSRSFEILTGEKEQNTEQLNSIQQGIRAQQDLRELQNAQRNATLKGYSIYAKKDKDIAKQRMDEIAQINDKKKLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K088</accession>
    <name>A0A8S1K088_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K088"/>
    <dbReference type="OMA" id="DFGIRRN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="88" mass="10285" checksum="CD8F34BDDCC9AF82" modified="2022-10-12" version="1">MSEELNSSLDSLELDLYHKPNPRIRTILNYLRSQNIKFCTDPKKKEEDFYSKQSSTEINSDMDFGIRRNRFINRGPQRSGIVLVSNTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K225</accession>
    <name>A0A8S1K225_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0130058</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K225"/>
    <dbReference type="OMA" id="NLQECIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000526">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000771">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000660">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="298"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="334"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="408"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="444"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8048740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="56016" checksum="EC360F2C731C753A" modified="2022-10-12" version="1">MGDKRIDKYLFNISHKIGEGSYATVYKGVNEKTGEKVAIKMLNKSVINADDYLREGLIQEIKIMGKLKSPNIVQLLDVMETSNNYYIVQEFCDGGDFDEFLKKKKNLTEKEAIKFLVDVLTGFTQLIKNGIIHRDLKPANILLDKTTYKLADFGFAKCVDNFKKDMMSSMVGTPLYMSPQILDHKRYNSKTDVWSIGFIFYEALFGKTPWTARSPPELLKNIRTQPLKFPTDKIPISQETQDLIIGCLQADESKRLSWEEIYKHPAVAQYFQDIVKGNSKLEDKAQYLINDIRLMIIKEKLDISQLFAELDMSKDKALDVNELGRFLTRVDKDIQRDEIEYIFNKFDEDGSNSIDFEEFKKWMEDNSIQVKQEKQIRDRSKKQSILGKNTVEDRANYVIEKLKLSIIKYNIQLIDLFKKFDKSADLKLDLKEMGQMLKRIEPSINEDETRAVFDFFDINKDGEITFQEFQTNLQECIVHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2C1</accession>
    <name>A0A8S1K2C1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2C1"/>
    <dbReference type="OMA" id="MNYEDIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8049007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="304" mass="35855" checksum="DB06E619053839A5" modified="2022-10-12" version="1">MNHSDGAQSPPLFGMYPLPYWPTNSQFLLMMNFQNPLMSPIHNLGSPILTSNINFPQSIINFDVKCDQSQILKDAPQQKQPQQYFSQIQTIQQQIEEMNYDDITIENLEQMIHLLFADSSKIKKIIQKLKEKKCVKVIKILDTLTKKIRSQSKSREELIKFCLRKAFREIFHEIQEKNTKSKLNLKAASKLFQQSYQAEKSKQISLPFRKNSKIKTMNNHFLHELFQSKQFIDQYKTFLDNLDAIIENDRNKKIKALAEKSWEFISSNRKAYTVKRLPWSIKNIEKLKETANELLNYCDEQQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2R8</accession>
    <name>A0A8S1K2R8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2R8"/>
    <dbReference type="OMA" id="RANKISC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF197">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8049177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="29726" checksum="0EDC5620AF7224B6" modified="2022-10-12" version="1">MLSLNKLIYLNPQPSYHSKTFDGHQKLKLLFIRTKQNKHQIPCLQIKANSDEYLLYFHSNAEDIGTCYEFTSALSNGLNLNVICMEYPGYGIYTQVEPSQQQIEKDAEDVFIYINLELRVPDSKLTIFGRSIGTGPACFLASIYQPKALILLSPFTSIKAVAKKHFFFAANLLKDQFDNVKRANKIICPCIIIHGKLDKFIPISMAEDIYKSLASKRKTFFYPEDKDHNNFNFSYDINDIVQNFIKEIEQLNHMFSPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K548</accession>
    <name>A0A8S1K548_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K548"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="189"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="321" mass="35929" checksum="170F3AA020B00C6A" modified="2022-10-12" version="1">MMQYRLNIEAQGLADEKNISDEEIGQARIAALSKVNELNQKAWASAKARREQIGIEYREATDYIAWATQRLADIDRRSVELQELRCFSNGLFVRAIKQHNDALGVIRILKNDLSGYLTGQPSSLVEVNVQNVSEKLKQYSQLFNQDAMTKFAQLAAEQASGNAELHALGQDEGSLSDRQPGHNIGQLVYNALSDLEDQLKGSLANLEANEIAAYYQLADWLADTESEVAHLNDEIQRKTQLQDKLVVQEQAALAVQAKANSVLKDSQNAINAATASLQELRDLYETELNRRNEENAIIDEVIHIFKQQVLEMANQTSFGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6Z9</accession>
    <name>A0A8S1K6Z9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E2F/DP family winged-helix DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0170132</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E2F/DP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6Z9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="3">
        <location>
            <begin position="10"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8050587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="183" mass="21733" checksum="420AF659FBEFE8C1" modified="2022-10-12" version="1">MMELSNEAMAYSDICQQLTEEMIQEFNGQYNDKQKEIKNLRRRVYDALNVMISIGIVIKEKKLIRKNTETQVNLTKQNLIIRKQNLKEQLQIKKTSATNQIKQQESLKKLVELNKMRDVDESEKIRFPFILVKTQLNNNDEDELVLEQNKSMDYLKVFSKNQLDLQLDLNVVQKLFQIEHMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7X4</accession>
    <name>A0A8S1K7X4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0170060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7X4"/>
    <dbReference type="OMA" id="HFEDKRE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019">
        <property type="entry name" value="KDA ANTIGEN P200, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="155"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="258"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="547"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="589"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="691"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="552"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="589"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="619"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8050443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="726" mass="88350" checksum="27DAD9C0F461C56E" modified="2022-10-12" version="1">MKISLQDFGFHQPNAPEVNTPRSLEACRREGILPNELVKTSFEDFQKKYKLSNLDAKGIETYYKHFEDKREQKFKDVIKQRHIVIEDEKQGLWNPDGLAKSKMVSKYSQQDVSSLIEKEKQQIEKIKKKQQKEIESMLEYELKMQEIREQNEKKLQDEKIKQKQRELELERKKEQQEEIKKQKEIQKQQKREQEEQALKERMKLLELKEQERLKQEELKLRLKEEENLKKEQEKKAQQEKLRMINEENIRLQLEALEKRKKDMNDKAEQRKKMMEDQKEKKKQEAEQARIQNETRIRLAKERNDIEILRVKQDVEKKLQISEQKRLQFEEEKKKKLEQQKLEAELHSEMIKKVIEQNEQLELERKKQYLKKIDEAEERRTLLEKELEKEKEKRRQEEHEREQQRHQVLLQNEQRQKDKIEEFLRKFQEKDENLSKIQLERSISLTDKRNHETLKRIDKKDNVERIMKKQEYDRMKLQEKLNEKMERADKIQEDLNQLLQQRQNMRKEIDKQKRDLLLKLEKIKEGKVPANDIQKQFGIQSSVLLEKQHQIPRRTQSQSISQDNLNQTKIEKPNKTIRISRLSDKLPEINQSSQQNLEPSSNSNTLQKIEQIQSSPTKSQNEKKPIKQTKKIDLSKSQNLQPPNPQVLFELNQIIQRQNQEIMRVFMEEQANENRRDQKIKICKPEVRAKMEKIYSLERQKAQQRIQKLQEQHFKEQQEFKLIHFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9S1</accession>
    <name>A0A8S1K9S1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein FAM184A/B N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0180113</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9S1"/>
    <dbReference type="OMA" id="MCKKVAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039478">
        <property type="entry name" value="FAM184A/B_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18870">
        <property type="entry name" value="PROTEIN TAG-278-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18870:SF9">
        <property type="entry name" value="PROTEIN TAG-278-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15665">
        <property type="entry name" value="FAM184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein FAM184A/B N-terminal" evidence="3">
        <location>
            <begin position="20"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="28"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="737"/>
            <end position="964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8051471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1010" mass="121522" checksum="8BA38A4BFBDB5AC7" modified="2022-10-12" version="1">MDLGELHHRLCKKIAQLTKVIFFLNTKNDEYESNLKAVVQAYEQEMDQIVKDANQIVLTYKQALDKQSKNDDIQQELKQLQEKVQVEKQTAQIEYSQYRKRMEEKEQGLVTQYEKKIKEIQKETEFQKQKFDDLSKQVDKVIMKQEELRRGSQKQLEDTIKEWKDKYDSLMRKKLEQDDQIADKDRRLQKQQLEIDDLLKKIEEEKRKSKEAQDRLQDLMKQNFDQKLQSLQNEINSLKQEVTNLKNQKDDLTKHNHNLQDEIHQLKDQIAKLTLDLKDIGQKYQQSQTDILTQKNENSKLKQINNDLEEKIKQLNSQIENLKQQLHAYQQDGSMKETQLTRQLNDLEQQLKSKDFEIRELHIKLNELQKKADMLQMELNAVRDASDRSNSDKLKEIEELKKNVRKLEDEIEKLQNQAKNQMGELEKNLLNKIEQIETEKRELIKRYEEKIQKITTEYELKLKELREEFENKIKNLENNHSQEIEQIKNDFNKKLKQLEQQLNDERANVEKSAGQAINDLKNQINQANLKISSLESEIQNLQNKIKELEQTIIYNLNQITGKEQEIKQLLQRISELEDKIRKIEMEGSNNQGQLQIRIQELEQQIKQQRIDLLREKDEQLNQQKLSYEQQIDSLRSQYEAEKKQIKIDFERKLQLKEEEIAKLLQQITSLKKDMETGQGDWARRLKLKEEEFEQMLKEINLKHNYEIELLDKKNTQMKEQMKVYYEKEIQQIKDDLNVETQNKLNQQSREFENQKQSLIKNYENQLQDVQQKYERQLNDKITEFENKLKQLQNKLEQEQSDHKATKDIISDLRRQLENALELNKNSSEKYEQQIKILQQQIKDLNIKYQQAETEFSKQIKEKDLWVQQMKDEAKQLEQQLRQSLNQQLKDSLIKQKEFADNLKNEFEQAQQLLMQKLQMLEEDYKELTRLYEERPSRPEDLELIKQLQSQVNLKDEEIKKVNEQMKYFKLELINRDNNYNKMFNVNPTIGTLNVLENKSKPQTGKMQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KD70</accession>
    <name>A0A8S1KD70_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190252</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KD70"/>
    <dbReference type="OMA" id="MNQDAFK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="300" mass="35231" checksum="FB28D160856A3DD3" modified="2022-10-12" version="1">MIFEISNYCKDISNCFISSNINSCQNGAFLLEKIQTLQDSLKEFDFWFKTQFNNEELLVNYIYTELLEVRKINKVVQFKKIDEKSSEIKGLSIDSQKSAETQSSICSAITNYRQYSKKLQKKGQILMELLNNNKEEQINQSNLQTPPKSYSLDKIYYVINFEINCLKQYEISGEEEIQLQKIQEKEILKWILETVQAKNNYKQILQCLINSAQDLANYQMEDFTQAIEKLNIQGSSFTNEEYKRSISNQSFQMNQDAFKQLIVDKIKQSMLEQNEKKQKKRISSNKYDTTDSKVCMCSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KH30</accession>
    <name>A0A8S1KH30_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">XLF-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0210227</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the XRCC4-XLF family. XLF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KH30"/>
    <dbReference type="OMA" id="KENIWQS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032807">
        <property type="term" value="C:DNA ligase IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052287">
        <property type="entry name" value="NHEJ_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015381">
        <property type="entry name" value="XLF-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32235">
        <property type="entry name" value="NON-HOMOLOGOUS END-JOINING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32235:SF1">
        <property type="entry name" value="NON-HOMOLOGOUS END-JOINING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09302">
        <property type="entry name" value="XLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XLF-like N-terminal" evidence="7">
        <location>
            <begin position="19"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="264"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="232"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="273"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8053967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="294" mass="34896" checksum="A56D54A9290B94CC" modified="2022-10-12" version="1">MNVEEEDINFLKAQPLWTGCRNTPYLIRAGINIDKCLIFITNLRQSWICKLNKDRIQKDKELYNKSIQTTNFQGVLDLLELALKFDPQAEYSFKLNESSLYYGYSNVIKIYTLTWVFDCKVVDDQYHIKILVNDIINPLLLTIHSLENRCNNYKSAFSQLEQDYIKRLNEKERAQYKVRDKDEDLSVYILDDQIDQEKICQINFNPVANYFIQYYAINKVNKTLQQMKKRQPQQQQNKNENSSSNIKKKENIWQSYENTLSDMFEGKGQQILENDKNESKQDRSKNTKKKVGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIB1</accession>
    <name>A0A8S1KIB1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0230069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KIB1"/>
    <dbReference type="OMA" id="IQKCQQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="225" mass="26587" checksum="065C0679D845702C" modified="2022-10-12" version="1">MKQEKNKSKSKDQREEILPINKIIQKCQQTLEITQFEQQELKKMFSIVSDKMHQVNQKIQTFEREERLLRRDNDEWEFYLKEKKNLLKQLTLLIDLISGKDHSFEIDISTNPAYKEIQNFSIHKKQQGEDLLKSIQNDETSIIKLQAQLLEIEEETKLLRQSGAFWNCVRCNITLKEGFNEETCIFHPGKLKYFSCRTCGGDEYFTCCNQCRDCNPGCKKGLHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLV8</accession>
    <name>A0A8S1KLV8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0220216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLV8"/>
    <dbReference type="OMA" id="CSFKKYC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="179" mass="21258" checksum="1CDA32E3CF49ECFE" modified="2022-10-12" version="1">MQNKVQFIESNNTEGILQVPDNLIGQVSKILKDQFGFSIEERVGAKSQQKQQVFQQQLNLIVQLIMCSFKKYCNINQFNTIKQEMENMQQKKQFSKKFSLYDLHQLLYENQFCQEFRDVFENYLSNGSAEKDLKQSRKMTQDQKKVLKTIISELLLEVLKNNPNTSLFKHQFNRKKFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQ20</accession>
    <name>A0A8S1KQ20_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0260090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQ20"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026306">
        <property type="entry name" value="RSBN1/Dpy-2/CEP530"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354:SF11">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354">
        <property type="entry name" value="ROUND SPERMATID BASIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="333" mass="38119" checksum="0798A3084D801B72" modified="2022-10-12" version="1">MEQLLFQVGGKDPQDRYYSFNGQFHRPVLRLGSGSFFDTLEEYNPFCLSCNPQPQKDCSSKGLIRAVSAKASQFNGNPVNSGDRFGTTEQYSVQGWFKWNGKTSEKDQLLFRLTSTLAGEDALNFDTLSCYFDTRDQTLNFYTYTYTDQLGSGNPEVRQIVEGKTFVKDWFHIYFAYSRKSRQADVIVEHSQGKGALSFKNVNHYVAPSLILYYGKDQLTDAFQGFIQGLNLFACDITYQPTPKPVEDCTPMKDPICLAGDQYENVSLTNIDNVVQELEKRKQLMQDKQFVLPTTQCFCFPQQGKKVIPQRQEGFLEIYDDDEEPQQQDSVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRC7</accession>
    <name>A0A8S1KRC7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="221" mass="26670" checksum="03EF2DE0AE3EB9A6" modified="2022-10-12" version="1">MSSLIKKDQKKAPNQYLDFDQLTHKKYHYDQKKANEQSIKQEQYIQDLKKAYDQQKLEIEPLFQKVELLQIERKQLMDEIEQYKMSQMEILKKYEYEYQEKDKLNKELTKLKQQIQAQQKMQLPKEFQDKKCHELKESLFVTIKDIMKDFLDNYNHIMGFKDEIELSNNLLDELLQQIDDSINMFVDNLPVQSNKQLQSEEIXIQCKSKKQHLDNAAQKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTJ8</accession>
    <name>A0A8S1KTJ8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0250334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTJ8"/>
    <dbReference type="OMA" id="FELIFPY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="311"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="469" mass="55832" checksum="910A3B1A4F54C41E" modified="2022-10-12" version="1">MESFLEKKSPVLIKGWQKRYFVYCENLLLYFKNKVDLPPYQPKGVIMKQNIVQVDYLDPNKNEFVIHVGLRKFQLRAESKEIKEKWLDILKVPFNQTSKTKSELSNMNKVKWLEIPEQTIIQLYLRREEILQNVIQQKTQDTQNSLIKSSYIQQTIVAMGEKNKEFILRLLVEKKSVKNPQVKKKRWLLLFTSISTESLDQIHQFIKFIPQQYTLDTINLLKYDNNLATLTKTTIEIYQIQNLQFVVTNEYQIMFMYNGRVFELIFPYLSDAKKLNDYIVKCQQINNSMPVQVSRTNQIPVYKMQDSRRRIINLKGKTFLENQVEKKINASQWKNVILTLKEHALFWEFVDTEEYSNIELKSISAIEQCNRMFTIIVNDQNTVQQQFRVSTIQEADEWTGQLSYMTDIEITIKVLEKSQTLQNYQQNALTNQSQTQQLNKQKTQQQQEEQKKPSFFQQLFCWGGERKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTQ6</accession>
    <name>A0A8S1KTQ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0250017</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTQ6"/>
    <dbReference type="OMA" id="HQNELAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14811">
        <property type="entry name" value="bZIP_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="3">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="244"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8056712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="383" mass="44837" checksum="8535A0CBC14C638A" modified="2022-10-12" version="1">MSHFDDIDFAQNSNSSNMHFEQEADSGKKKPKQNTKNTYIKKISSLIKMTNQEILEENQEKKQTVHKVQSQQFPGNNLGDKEDKLAKNRESARNSRRRKKIYLELLESKVTKLSEQLDVYKNVNERTQQLAINLQNKITQKREQDQTKMILFSNLQNSINGNVGEMNIDAIIDSLNKKFGSGSQERHQLIDHYARQIYENCLAPYINYIIGVAKTEQDIFSNQEHTDNTILRNLKLTDKQKQILQKKQQKLLRHQNELANTLSSIQEVKNQIQAELSSFDQTLEQLRRELKPSQVARFLLSIEKKDMQHHFKEQFEKCFGSEIDEDDSLELYQFMTEHNQCNSLGIDIQQTYEIYRASNDFLNGKAESEDQQQQSLQQQTKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KU55</accession>
    <name>A0A8S1KU55_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0240215</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KU55"/>
    <dbReference type="OMA" id="IICCACK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000044">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000051">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="4"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8056246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="314" mass="37030" checksum="E247C45397A101E4" modified="2022-10-12" version="1">MVDCNICFCTYKEEECYTLPNCLHKFCKACLTEQLKTKIESQQIDLSDFKCPQCGRLFNPEIIENFLPPNLFQKYCDFALQFNKIMGLEDHELLTNCLNEKCIEKFIIWKDAEYMQCPSCKMKFCRKCSLKYHKDEGITCEQQKELHKDEFYIEMKKNLKICRCPKCNNMCEKISGCNFMYCRCKANFCFLCDAELTEKYHSSHFQKNDPYNSPCRVWYNGTWVDPDKVPKVEPIKEDKIVQVIPMEQKENDIPCPNCGNKSKEVSQLVFFDKVIQCQSVKCQNQAFCISCQKKVPIDDMITHFNQLSLECKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4U1</accession>
    <name>A0A8S1L4U1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0320154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4U1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="332" mass="39257" checksum="29692781ACC52C29" modified="2022-10-12" version="1">MNIDLIKKIKKNQQKIEQHNQKLCAQAKDIYALVLDSNQQTIINVNSNLRTLVWKGVKQVQKNQQNRILQFQINLSFLVLSIRQEQYMTFKFSPISVKQSHIDKIVSISPNRNDLTRIRMENAVLKEQINSKEKYIDQLDETIIELKKENLMSQYYQNTTNRINKELEIGKKNSRSNFQNKTQRKIKVMILIQMKWKPLCRLQSQKMNNQSNNKKDDSKWLSQLQEEFQIFYTKSQETKNKMDDTKYLIYQLSEINQFLTNKYCSYVQIIKQQKVDLTPLPKASSIKPFAQVFQVQIKTLVNLNQILKEVSTSVEKFSNQYISYVGLQLVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6X7</accession>
    <name>A0A8S1L6X7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0320271</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6X7"/>
    <dbReference type="OMA" id="KQGQNMT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="566"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="430"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="445"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8061985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="833" mass="98801" checksum="4A0231F6D32034B3" modified="2022-10-12" version="1">MYQEDTLNINQIQFNQLLLEAHSQFQLRDMNQAIRLYEEALNFQEAGRDIDRMAIILANLGIIYFHNCDYKQATEKLLQAFTMLDRKNDFKCALLIKILGNLAIIKLITTEFQECKDYNDQAIQLILSVQPPLQYQLFKELMYIYYRFQSFEAMGDGNFENLEQKYDGQSLACFYSSMGLNRELCGDMALAFKYHQKALKLWYDQKENGFIVITLRRILTIAQNERMDCKDYVQQLQKCMQSPELRGISPEILFKDCEKKLQCAREITTSLQKLEQQLNYQKNQQLLSQPLTVKQQDEFWKLALKLRLKRAIQYCYHQMSQQEVENASLLSQSIAQLEHSLKLLDQQQPYENYLQNLPFTKESVNIIKSHLLKFNRICLKIQLQPFFNYLDQLKQEDDQGQQLFQQPQFNSKFQTNQTYNQQIQNSHYLTQQQPQSLSNRQKQFDSINSQYNSQQSQVNNDYQNQLNIQSYHSTNDQQNYMVRGQNSNQHQNNQYQQFNNPLQSNYSQNNQIEQKDFQSQKYQTVYANNYEQQPLKNSGQFGSQIMGITGNSQPKINTVQSQYQQQQQYQQQQQQQQQQQQQQQQQQQQYQQQQQQQQQQQQFQQLQQQSQSQQASLKFSKSQLNPSQIKQGQNMTVSLTKSQKLKNVSLLNAAYRTVMLGDQLTKCNKSSNGRIERFFILANDGTFRWAQNNKHINDPKSVNSYSVSDIRGLLYGKVTDVLRKSYNDKLEPWLCFSLIMKTRSMDFYAQPLQINSWVFAMSEEIKRRNPLAFVITPGKMLWRKINIILHYYFVDRKKEKEKGGNKKRKQRTNTFVHLLFLYAKNQYRLPQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCS1</accession>
    <name>A0A8S1LCS1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CHY-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0380146</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCS1"/>
    <dbReference type="OMA" id="FDECDEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045041">
        <property type="term" value="P:protein import into mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052604">
        <property type="entry name" value="Mito_Tim_assembly_helper"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28082:SF2">
        <property type="entry name" value="CHY-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28082">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8065858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="97" mass="11495" checksum="D52CB0BD636F00D3" modified="2022-10-12" version="1">MCKHILNAQVAIRAPCCKKWYDCAECHQELEDHPLLKSIEMIFACKKCKKVFRKDITDYDESDEYCPHCDNHYVIKAVTQESKEIQKFENLVKEVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDN4</accession>
    <name>A0A8S1LDN4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0380319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8066198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDN4"/>
    <dbReference type="OMA" id="VESMQIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="176"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="51485" checksum="CC640EF82FE85081" modified="2022-10-12" version="1">MNSYTIKKSSHLINAQDLQKFKNQADQIKKDFKKLIEMIDQELKRFSNFSNFLDEMQKMIDNYQLNSQDNLFPISSEQLSNELNSLIKFIDNQKNVINIQIEKNQVFNQFVESMQINKNEIKQFRYELQNQTQLDEQHCYAMDFNKDATYLAAGFINGDIKVYNFNSERLQLIQQLNAHNDSIYCIVFMKKSNQFISGGGDENIIIWEIKNSEILNVKKKFQKDLNQIFCIITNIKEDEFISGSHNGFIQFWKINEDQYFQTLEDHKDRVLSLSLNQSQNYLISCACDRKVLVSLFENNQTWKVTQSILLDNIPRRLCFINDITFTIQCDTQKCLDIYQLLENEKRFKKTQEIKVKGEQDCFFGFPQKFISSKQQLLSKNGNQVNFLIINKKNQFTLFHSIDFLTGQLWGTISDDGQYVATWDNISCQIQIRKYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFR0</accession>
    <name>A0A8S1LFR0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I NDUFS4 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFR0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006885">
        <property type="entry name" value="NADH_UbQ_FeS_4_mit-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04800">
        <property type="entry name" value="NDUS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0249">Electron transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="186" mass="22085" checksum="A623972795C1A65B" modified="2022-10-12" version="1">MSIRQFVGIQEIAKKAFNSLIVIRKEIETKNQEVLAHGISVYNQVVPVDASRSKFGFFRSSRLDNRSDHRYYDMNPLPAPESKIARVYIMDDNRMHQNPTMKQGYHWNVQIERGPIHRMPWSGWTFSKDQKSREVYKFVSLEAALEFCQQDGYGYIVQYPHFRYTSKRKYADNFKWKGFPKDEQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFW6</accession>
    <name>A0A8S1LFW6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0380060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFW6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9614" checksum="12B54F100C15416D" modified="2022-10-12" version="1">MNNKEGIGKYLKLNGQSYEGKFQDNKINCVGKFIWGNRRSFEGQWLKKQMNGKGKKKWPDGKVYDGFYQEDTLENIRYLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJD8</accession>
    <name>A0A8S1LJD8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0360320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJD8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035841211" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="231"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8064826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="40153" checksum="0AFBA292CCE934A0" modified="2022-10-12" version="1">MQKIALLALVLAVTAAKFIDTHTTLAQINADPFGHVVLSAIKAHLQASTPANEVNMLLNGVGAGLVQDQNDHDHAFELDTTTNNRIVEDLEKEILYHQNQIASNTQLRDDTIEALAVSEEDIRVTIQDIANNEATYAREEATRNQQHETFVAKVAAIDDVIDAIDEAAKLIQHLSLGASFAQLKSKYDVLHKKLSDNTSHTALLQPVITALTELATHGVNQKALTKIAQLLSEIRQQLVQEKAAKTDVEDRQAAHWAEFSVHLSNEHTRLVERKAQLEVQIQEQKDTIEDAQSWIEFHTLELENSEERLAGQQAWYAVQSEIYETQTAERTAQQEIVDRLQEHISEKLSTTAQFIASRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LRK4</accession>
    <name>A0A8S1LRK4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0420241</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LRK4"/>
    <dbReference type="OMA" id="NIHIIHR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="33"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8068601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="503" mass="57851" checksum="27B711C2C1243A01" modified="2022-10-12" version="1">MANRLKTQIQFSDDQSNLNSLEPIETKRFEDHYECLQTIGQGAHAIVKTARKKGTEEIYAVKIVRSGDQEIQNNVKRTFNNTRCLRHPNIAQDIELYINEKSETSYLVMEYCNLPSLESVIKKRTLKIEELKVIIKQLLFAIQHSHSKGICHRDLKPDNILVNIEENSNPPHVKLVDFGVSRRFISKGQEIDMLTKTGNIFYCAPEIYHKASYSKEVDIWAIGIICYQCTFQKLPLHSNELSDFVDLLGNPEKWTFKDSLCNIEMPLSNLILSMLNPKKENRITVEEAIRHPFFEIGTIRDVMALLNKDKIELDNCGRCRSLQNSLKLNEQWGDVIKKLQSSSNEDENINMNDLVRDFGNIHIIQRHTDKCCLIHLMNSVGSSNAFMSKLGSRQEILDKSCGLQFCGSSNKTIHQDPFGDVCNIQSSLEMKEEQQNSNNRLFLQLGTNLDYSLEVTPSEWQLTNSKQNIQQQQQGTKKRGSQVNRMFNELGIKELDEITEDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSM7</accession>
    <name>A0A8S1LSM7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSM7"/>
    <dbReference type="OMA" id="NRRFITQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="114" mass="13486" checksum="8CAD3EE4F834A424" modified="2022-10-12" version="1">MNRRFITQYPLSPQMNDISNLYKKSRIQEQLQFFDEIIKKVKFKKPVPQPKKIKLPISISSKTIQLQSERSLSIRSPRIKELSKKQMLPPISRQKASISGWEVNKSNDSFLYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSR4</accession>
    <name>A0A8S1LSR4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0420232</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSR4"/>
    <dbReference type="OMA" id="IMSFCKD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000006">
        <property type="entry name" value="50S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="9">
        <location>
            <begin position="19"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="8">
        <location>
            <begin position="82"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8068583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19469" checksum="187342EB304634D8" modified="2022-10-12" version="1">MNLIKKKLQTLPKDYRAKIMLRLYAGVAKPSPKMGQSLGPLGINMMQFCKEFNAASQAFRPDVPLRVQVIAFPDRTFKFLIKPPETTWFLRKATGTDKFTNFPGYIWYDTISLQQVYEIAKVKQEMDPHLKHVSLPAICRMIIGQLASLGCNLSTQYFKPETIVRPEIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUY9</accession>
    <name>A0A8S1LUY9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0470077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="LMCSESI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="807"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="850"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1009"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1033"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1110"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1330"/>
            <end position="1354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1391" mass="165686" checksum="5260A3DED00E1046" modified="2022-10-12" version="1">MIDFFSQWIEELRSKQKIKQNKSNFKASHKNHEKILSLVIQIQLYTISLYELDFFMLSIKDAIQNLPDSIQILTFSFFFLCWKLIYILLSLMCSESIHFPSSPIHFNILSIGQYFNIFLIVINLYLNHEQGEFQVTLSRIINLIIFGFYIYLECQKNINSFHTLILLLLTYSFFNNLSYFFIVNTKEKEYQIALLFVLCPLFLNLVIYYSRQSTKITFQKNRKIAQIYNNLLRLNEQNKNNMKQDIFETLMLKQITIYAKNPFHSELILFNNKIIKSKKNHKAFWYYKYILIKFSQKQYTKTLILIHNFKEQQFYSEVNQQFIRVQSVISFIQQLKLIILKSEVYELLNYNINKNLDLDQLNTNSFINYKVQIQKTQFQILNIFKEKEELYSQLLEGKSTKNNSIFNKSYNILKQALSISQDLRLELIVWKTYRIHQLLTILYVEFFNNIIEAEILKYFQLNNEELKQNPLENMDFLCLKLEDNLLNIQIKYFSQSLLLRLGYKVKDEQYFQFKMLFPDVFYEIHQELIKQFLESGTCKYLNKISDMLIRGLDNIYYYSLFQLNIENSTQSELSFYVFIKINEEQPNSIFINSQQQIIGYTTSFLKYIQQKYINFTSKSLYLQELSKILILNKYHIGQKYIKGYLTQNQIEITLELIERKNFVELLILDENIDYLLNQEIFSQEPSQQCSLMFNQESLVLIPNQLFIDELNSPLSLEKRDKPSQELIENDIKQQESIKKCSNINSQSILDKQKKSKMNQKGQQDDINNDQASSKFSRGFLDNSIFYEKYNKLNIFVSQSTRNRQITITLFLFLIVLLINIIFQILNLTYFLPQYKQVNSDIDMLTIKGNIMAPVHNCVTAQVSVVNYILLTYFFKEISQEVAFDKMQFGLDSIIKSYEHLKQSFSTQLDNQNIQEFFYDREILMYQLQNLSLVPRNISLKSALIYYQESQYFISIIDYYKVLQYVPSNEFVYYMANIITVDDYFDYLSDEVSIFVESRIQTFQINQTKFFLKMLFLELLIAILGISFFLQNQYNISVLFSLFTNINTFALQKEIQKLQKMIQLLIHDDAYQVYSFEVNIWEKEIQAESSSYNQKINLNRIVNHEYKIPRLGLLISFIIILIIYLLPQLILHSINNNFFDKYSQSTDLLLKLSQLGSAVTAVYEIREINYMISGKSPFFDFFTDQKRIEQNVKVVNEIAKISDFLQFYLFLDFDSSLTDDDFIGKFEQVQSQNLCNYITHYNQSLAEQHCNLIYNGVMKLGLINTLTEIYRQIKTEYENTQGFQMQYRNKSGAFQEVEIGLITSDTILYLKSEVFHAVILKTSELISTHQIFIGIYISISIMLAYIMIHYFYPYLLRRTTIIKNMIFLIPQLQFYLDQKFETDFRLIQMQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LVE3</accession>
    <name>A0A8S1LVE3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0480163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LVE3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="224" mass="26695" checksum="E1BD36B938E4862E" modified="2022-10-12" version="1">MRYINVIQTPKSEQQQIILPNVRVRSNTMQQNKQQVNYKKQITLNNDQSYNIGNELNSSRINNIFQMEIMTHKFEQLKVSLSHSTQEDVNKTNIIKSKKKKTIQKTQCNESIYRINEYQLFKKKIEDTIQTELKQIQESRQKLMKCVLNTRQRNRSLQLYGDDLTPVIQNEKIILQNQKDEKEQRYLLDSIKKQYTQRSPMGNAYCCLKALKKTQQKKVFLKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYN2</accession>
    <name>A0A8S1LYN2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0510283</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYN2"/>
    <dbReference type="OMA" id="FARQNQK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="306"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="682"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8073868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="832" mass="97576" checksum="9E594007CAF55F8D" modified="2022-10-12" version="1">MQISKEKRMGAMLIILSVVDFGYCFDNITYITLILSCLLILCSIVSVLFANKKILSLIIITMYYGKELTLYGPTLTWPVLYLFKLIGSQKCIFLLLFSNQIVFNYLDSLWVYNIKRKDHYCIQLTFILLFEGYSQYVKQKTYYQCIRLLIKQFKQEFVLQIFDLNQRNITENAHCLPITNRLQTENTLIGSPRGDKINLIQDDKDIHYKLFLNNDLGFSIELNQQPIQQQVTFRSTKELLQFLQKNNNYFILAIQNDPLLNAKFQYQVRYFQFKNQQILAFSKIDNCLFARQNQKILKYKQNLINIFNHKLKTPLNSAIGHLITAQEDNQIIDQIKKLYLQPALLNCRLQLYLVQDILDYLSIEIEQLPLMNNKTNLKTLLLEVYDLIEFQCKLKNINILFKINNENFKKIETKSLFIYTDSNKLIRVLLNILNNSYRFTDEGGCIVLEIRLDQMNKITYFSITDNGQGMDEEQIKKLNFQLKSFDSTTFNKLYKQQDNNRTIKLGLSLFLANKLIKLLSGDSSCLQLRVNNNQLLFTFSISDIHEIQSASSLGKSKHNSFVKQKSLRGLNNIQFNSQSSPRISKFKQCSQRQIELKNYNSLRLIYQSDIHNEPQPQIPNRIAIHQNNPKFHHQRQRMRTKKRSLDYQFRSRATSFRTHQIEQIIRPEQIQQQQHLIQNEEFILIVDDEPFNHETLCMMLKNMGFKNFLKAFNGQQCLDIVLENHNKIYMIMMDIDMPIMNGIDATKQLEGLISNNTICYIPIIGCTAHEDYESHLQCFDAGMIHVVVKPVFIKSIKEAINKISELKSNETIKEDTSSDVINRIQSQSFCSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3M9</accession>
    <name>A0A8S1M3M9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8075000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3M9"/>
    <dbReference type="OMA" id="VSHFKKW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="174" mass="21073" checksum="91ACCF624AAC2A21" modified="2022-10-12" version="1">MINKMSNFRPYKYSQEELTISKKIKIEDQQITLPLTVFQQLVEKAKEENQKKEDTKNLLRNFGNALNRFILHNEQALKIITQYIPENNVSHFKKWVTNQKLENFEQFKRIWTVDGDIYKKIFSELSFEFYSKHAYSYVMHSQMRSESTKLTHLKYITRFLEGIVCPESFYYFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4N3</accession>
    <name>A0A8S1M4N3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0470240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4N3"/>
    <dbReference type="OMA" id="IVTPKCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="158" mass="18632" checksum="F5DA7F9E0C236149" modified="2022-10-12" version="1">MSRLYTLEDEQLDYGLDYESVLVVKEIDQRRQEKGNDNDLQQVLSLQFYSRHQQQLKTTVNLRSIQRLITEQKENNKKSKENKPINYKIKTPSLKPYQGSIVTPKCQTKTPQLTKSLKSTITITPLNFKDSLQKIKLLVSKQQQKHQLDFNKFKKSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M6I3</accession>
    <name>A0A8S1M6I3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0550013</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M6I3"/>
    <dbReference type="OMA" id="ICLPPGF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001413">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052788">
        <property type="entry name" value="RING-type_E3_ligase_ATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798:SF97">
        <property type="entry name" value="ALCOHOL-SENSITIVE RING FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="90"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8075707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="136" mass="16665" checksum="CF0B7D6923ED4270" modified="2022-10-12" version="1">MNRRKNQQNNNEYDKIQEIKKRICKTQTQYPLTLWHPMFMYNFYFNFNFICLPPGFQQKYKLIKSRKIKNWIDDIPILVGVDDFEQKETCTICLSDLNEKNIIKILKCNHFFHQECIKEWLQLKAECPTCRDKIHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBB5</accession>
    <name>A0A8S1MBB5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prefoldin subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0570142</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBB5"/>
    <dbReference type="OMA" id="YNWDVAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23156">
        <property type="entry name" value="Prefoldin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000043">
        <property type="entry name" value="Prefoldin subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016655">
        <property type="entry name" value="PFD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016396">
        <property type="entry name" value="Prefoldin_subunit_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR016396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8076959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="184" mass="21468" checksum="A42681AD41246EF6" modified="2022-10-12" version="1">MDLVTKQAQNNRKIPEAIFFENIDELISKNSVQRLMESLQEAYNKYKFMEAQLVKQRESMQNKLPEIERALSIVEHLENQKEDEVVDFLITDTIYSKAVLPKENKTVALWLGANVMVEFEFNEAKALLSSNKENASSNLRQFDEDIVFLKDQITTIEVNIARVYNANIRIQQTQQQQQQQQQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBI4</accession>
    <name>A0A8S1MBI4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0540031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBI4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="157" mass="19193" checksum="BC17616D49D6B08A" modified="2022-10-12" version="1">MISLIEVQCHFKSKKYPNLCNEIYFSSIQRLNDLLNLQIKLNFQKFISMQKKPYKFQCILRPKMFSYQRGYYIGRVIEQLQQTIIKSIFKSRYFQYYHQFTCFDNNNPLLFTINVSYCQPGLFCMLLFQSYSEIKSQIIDRKNRKLFLICHLFWEVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCJ6</accession>
    <name>A0A8S1MCJ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0590083</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCJ6"/>
    <dbReference type="OMA" id="LYAKWTQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003889">
        <property type="entry name" value="FYrich_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003888">
        <property type="entry name" value="FYrich_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040092">
        <property type="entry name" value="TBRG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22715">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR BETA REGULATED GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22715:SF0">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR BETA REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05965">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05964">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00542">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00541">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51543">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51542">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="129"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="392"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8077937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="741" mass="87011" checksum="1C1C2A100593BE51" modified="2022-10-12" version="1">MLYAKLQSYVGEEFLIKTLPASLSNFPSTRGIRVMKCPNNPLIIGELKLSEELYQFSIIPHTELQIENQVVTKETILNNFSRIYIPCKECRIKHYYTFILPLREPLKYEAQRAAQTQFNRVHDKVLYAKWTQEDKEMLKKLILQFGYGRWNKLQQLKFANRTKQEIKAFANSLLRSIVELLVNYDLGAGIVNLIEENPDDPYIETNQKDWELQNLKQKLISISKRILMLAKIREFIKKFKEHQLKNLGIKDKSQLKWDYLLGFIPSSSFYSQRPSIWWTRKHDSDLIRGVYQYGYTNHLMIKEASDLCFKDLSTSQNYQEFPFPETLNKRVKKLVQIIQKFDGFYDFDNLAQSDDEEEKSWSIAEKQALFNLLCDYGVPIGVDGRQNWQELKDKLILKINKFDKNLNQLEKMVQTIRTRCEQMLMKHKEREFLESDSSELEIEQPPSSIGEIRDEQGDEFQISYQDSQKFSKQTNMLHFIRKNILPQNQALFQQHIQSVEKELFIDIPEYDPKIHDIQIIITLSQTGFNGLQPITLNNINIIMTTEQILNRIEQLCSFFKKIRDQTNIKRKPELKDDQQKKIKPNTNGGLNEIQLPYQVSTSLKLVSLGRIIPSPAYHSEHNLFPVGYKSIRTHASMFTKGKRCQYTCEILEGSDGKPLFKVTSEEDVDNPIIKNSCTGCWVHIYNKINELQEHKKSKVTISGTDRFGLLEANVQRYLEELQNAELCSKYKFKYRNVTQDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDN0</accession>
    <name>A0A8S1MDN0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0510042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDN0"/>
    <dbReference type="OMA" id="RKGPIHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="118" mass="14016" checksum="8C81BA6F6A289806" modified="2022-10-12" version="1">MKTRNSVRQRQREDLSVTCQENVSISEYPLPSAAQTPIKRKGPIHKKPKQTMINLKKNKSQRQFICYDDKDLNIPQQYQSILQKHKSDDDRESDEEQIKHAINYLYNELLLCLEKEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDW6</accession>
    <name>A0A8S1MDW6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0560175</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDW6"/>
    <dbReference type="OMA" id="WSHQIAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08215">
        <property type="entry name" value="STKc_Nek"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="40"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="318"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="408"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8076511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="675" mass="79727" checksum="55619454A9F90FA8" modified="2022-10-12" version="1">MKQRPTTAKVPKTIDQREIQQLHDLDSHQYQLIGSNLSQFTILNELGKGSYGVVYKVKSSMDGNTYVLKKINLTHLKPKHQAEALKEAQLLRKLKHPNIITYYMSFIEQDNLCIIMEYAEGGDLQKLLKDYKERRKFMQEETIWEMSRELSSALQHLHENNIIHRDIKTLNVFLTKDKHVKLGDLGVSKIFNSDTALQGTRVGTPLYLSPELVQHQPYDYKVDIWALGCVVFYMAALEPPFQGENLIALGYSIVNRAPKALPPQYSTRLSQFIWKLLEKIPALRPSIQEVNTIYFQQRSQPQRISQQLDDVLPQVQYQQHQLQRPQTSNPKSYAMQAQETEKMKKTRKPQQQDLISNGSDTIYMGDVIDITQKQVENEQISRAQQYQQLLKDQQEKLEKERLERERIDRERQERMDKERQERLEKEKQERLERQERIERERMLRLEKERQQKEQQEQERLKQLEQQEYKQQQQQQQQQQQQQQQQQQQQVLKIQSNHVELYIQDIDDKQFQEERKNSFSLEQAPKFKLEIKHTKPQNTYKSNVQIRPLSAQARGSSIANISCRAKLIPVQQNQSVKEVKKFTILDLNPNSTQPSLHQDMPIQQEPYIKYPNTNGGQNTVINNVNNIVNNQPNKTKENIRIKSALIQQYSNKRQQQDQQQSNQIVKHYTLKDLVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ME31</accession>
    <name>A0A8S1ME31_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0560215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ME31"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="12544" checksum="38138A8DA8EBED76" modified="2022-10-12" version="1">MNNNYSQDNYVLNKDTLKQYETPLPFETQDEKTQNMDQFQCIYLCLYSQEYIEFQEVLVRVDFLKRVYLLLTLEFLINFGMIALGLYKKLLNLFVNTNFDFLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEB9</accession>
    <name>A0A8S1MEB9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0550220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8076073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEB9"/>
    <dbReference type="OMA" id="IEPMRMS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="124" mass="14574" checksum="9FA5D6E9566BBCC6" modified="2022-10-12" version="1">MRLNTLPDVLFPNPIQHPNYKISFLKKVKEEILNNQFQNEPMKMSRQNMTIRSLKRGSIRVVSASIVTSQNNTQTNFRSGSSKFKMIKEHSQIEEMDETFSRTRTPSQLVRRLKRGLSMFEKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MFW9</accession>
    <name>A0A8S1MFW9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0560091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MFW9"/>
    <dbReference type="OMA" id="FIEVCKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="181" mass="21200" checksum="A31B659BF637498C" modified="2022-10-12" version="1">MSVSPPKSQSLSADRRKTSLFMYPFPKPPSVSHLPIFNMTINKENVQPQKQSWNMYQQSQQLTTVSQKKKPQKFIELESQKDPKVKYKFPIFTDNQLGIKLEYQQLLQETYDNDDDINTRESVMQYFIEVCKQDLVQGMRENQTIKDKKGNPIINFAGLQNRLKEQYTKIEIDAQIVTDAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJF3</accession>
    <name>A0A8S1MJF3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TRASH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0610088</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJF3"/>
    <dbReference type="OMA" id="TCYFCSG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000001">
        <property type="entry name" value="probable ribosome biogenesis protein RLP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF8">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN RLP24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="TRASH" evidence="5">
        <location>
            <begin position="6"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8079032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="171" mass="20601" checksum="14A88AF4CCDA17D9" modified="2022-10-12" version="1">MRIEKCYFCSGPIYPGHGIVFVRNDSKMFRFCRSKCHRHFKAQHNPRKTRWTKAFRKAAGKEMTNDSIFEFEQKRNEPLKYNRDLYINTVQAMKKIDKIRLKRENNFWKNRLRAQRKQNLDNVETELAKNINLVSDKSVKEIIKQKAIEKAQLKAQKQKRHQKKDMVEEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJU8</accession>
    <name>A0A8S1MJU8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0570192</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the IPP transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8077056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF60">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8077056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="408" mass="48143" checksum="716C25B09F827CE0" modified="2022-10-12" version="1">MNNNGFIVYIIGTTAAGKTNLSLNLGLDNYEIISCDSMQIYKDANIMTAKVTAIEQTIKMHHGIDLLELESEGFNRKQWKHMTINKIEEIQSKGKIPVLVGNTHNFIESILFNQETEENQIQQAAEFHLDGKEPFEYLKEIDPLAALKFHPNDHRRIQNSIKYYQNIGQLQFDQLDHNSEQFKLRSNNFLIIWPKWRKNEDLKIKVTERIQQMIEKGGTEEILRIFERLENKNNKLGGVLKGNGYCQFEKVYQYYKQSGLQYPNIDENFQVLLKQGAEKLINDTMQYTKKYIQWIKNRILCNSQIDIIANRLFLLEFDSIQTFQEQVIIPAQKIYNLFYQQFQDDQLGDEQFKISQQNLGNIKIITPEMIEKYKQIQKQKSRNXEEPLKYQSFEIIYKIYTLVIDAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MM81</accession>
    <name>A0A8S1MM81_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tubulin/FtsZ GTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0630249</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MM81"/>
    <dbReference type="OMA" id="QCNSSNY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06059">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000044">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="5">
        <location>
            <begin position="34"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8080412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="423" mass="49484" checksum="A2607B680FDBD7E6" modified="2022-10-12" version="1">MSSAFHIHIGGAGVMIGDMLWKLYEKEHNETTQKNYIYQQIDDHHHPLALFADLDDRMIHEVQRNKQVQFKKNSFLYGQEDASNVYARGTYTLGREIVDKGLDFIRQQVEMMDRLDQFVITTSISGGTGSGFSELLLSRLNCDYGDKVKKNGFIIFPSSEMSNNILGVYNAMFSIQMTREYFQSITMFDNQSMYNVIDHQLDLDFVDYSHINNLVAQIISQYTGLRRFNSSDNSKFFSNMCPYPQMHYFIPSFGKMTLINDYNRKELDQSQFIKYLTKKELKLYQCPQNPRHLSTTLLFRQKEVNHFYGKFDLTIQNLDHYFNQSPRIFQCNSSNYQIIPEFAEMKKTGTFFSNDASIISRFKLLGAQFDKVYTKRAFVWCYVTEGLEESEFSQGRESLANLENQYYEIKGVNDSQNAFYSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MMC9</accession>
    <name>A0A8S1MMC9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Bms1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0630283</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. BMS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MMC9"/>
    <dbReference type="OMA" id="KLHVPMV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000105">
        <property type="entry name" value="BMS1 ribosome biogenesis factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BMS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="13">
        <location>
            <begin position="40"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="479"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="860"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="313"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="479"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="489"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="860"/>
            <end position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8080472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="913" mass="106960" checksum="3CA3279EE421A37B" modified="2022-10-12" version="1">MSDSEEEQVKQAPIIKPHQKQQKDFYAEQLQANEEAESQAPLLVVVQGPKGSGKTTLIKSLVKHYTGQKIKKLVGPVTVRSNKQHRVTFIECPSDINAMSDLSKVADLALILIDASIGFEMETFEYLSLLNNHGFPNVMGVLTHIDFFKDNKQLRKTRKKYKKRFEYETGGNYKLFYLQALKNEYYLKQDIHNLARFISIIKIAPVRWKTEHPFILADRYEQGKDNTTTFYGYVRGCTYRLNDRIHFVGQGDYYIDSFEEVLDPVEIITTQKKHRSLKDTEKLVYAPMSSVGALTIDATAGYITIPQPIFTEKNKFVNNEEQDDEEIEEQEQEQELPEGVRMVRELQQLTEGIDKQLEEEEDAQLLEGFELEEDQPIKKKNNYQQIQQQRELVRLKNRVNIKIENGTVMPISDTLLATDLHELIYQNKNGINEFDSVRYIPKVSDRDSYKEIKELFVTGFYGQNDVVDLENLEKQIELEEQDQEQEQEQQEKKDNIQKDDNQNQKDNQQQRQQLVNELTNSNLGLYKKGTYVKIVIKDFSSSIKDDYPMILARSEVGEDNLGFIKIRIKKHRWHSNILKSNDPIIISLGWRRFQTIPIYCVQDPNDRLRFIKYTPEYDYCYAIFYGNFAPQGTGLVCTQSLSNKLSKFRIAATGIVLEMNHQFDVMKKLKLVGEPFKVIKNTAFIKGMFNSSLEVAKFQGGLIKTVSGLRGHIKKPSKLGPDGSFRATFEDKIQMSDLVFCRTWVRMHIERFYRLITSNVKLMKTMWELRQEKQIPLEFKPESTYQDQERVPVKFAPLRIPNNLQQNLPFESKEKVKSLSFRERLRQQVEKNLPVKSNMTDKEKEVYSLIQRLNTIKNEKEKKREQKDINKQKIKEAQFKGQKQHLEEGKRKYKQQKQKDKFIKQVKKQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MV35</accession>
    <name>A0A8S1MV35_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0690200</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MV35"/>
    <dbReference type="OMA" id="SNEGWSN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000819">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="94"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="11">
        <location>
            <begin position="342"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8083272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="50633" checksum="B8CC755626820FA7" modified="2022-10-12" version="1">MRIGQFDTLQLMHLRHPMTETKLKIAIRRYSSPHKNNNQDKNDISFIGLFQNINMINRRRMSEKKAPSKYTSNTRQHSKDNSVEVDVALNKHHFLMQYVIGVGGFGRVWKVIYKKQVYAMKEISKALVLLKQSVHSIMRELQFLTELRHNFLINVFSAFQDNQNLYLVLDYLGGGDLRFHLGRLRKFTEEQTKFFAACIIVGLEYLHENNIIHRDIKPENLILDNQGYVRITDLGVAIKKTGENFETSGTPGYMAPEVIFRQDHGAAVDFFALGVICYEFMTGRRPYYGNRREIREQILAKQVQIQRSNEGWSNESIDFINILLQRKPQNRLIYPRQHKWFKNFSFELLIQKQLKAPFIPNNQDNFDKSQIFYEDEENDQIIRLHQHLIRQNQEQFQGYQYQEKQQQQFIIKSKKSIINNTKIFQFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MW47</accession>
    <name>A0A8S1MW47_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0720081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MW47"/>
    <dbReference type="OMA" id="KNNTLHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="170"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="423" mass="50786" checksum="AE462E90EF8EF718" modified="2022-10-12" version="1">MYLEFDEDFYQIQNYVKQVNSNLVVLPQDNVESEIELFEKMILQIKIYGSKIIELYQSLDEICPTDRQIQAIIPQTKLEQIDYDQVWLALNKGFVNDKYKAMDLMQQLDYTIKLNQTQQAKFIEFLQITCRCKDRELEKLSQLFQNQYPESYFKQIEYLTEMLHQYSQMVPLLVQENENLRQEIENTKCNDNDGQSNEISQLRLHLQNIEKQLTETQSQNEYYQQMIKAEEQVRQNKTQLQMLQIDNQDLIFKNNTLNEEIKNYQDQIKTQNESFDRMQMEYKKIIQKKNQQIQELEEKNNLDLTIKDNNLVDIDLIQQKIQQFKQLYEQKIKHLTGTITHLEQENFKLKDLVRSSALEVDSLRLQIEKIIQSNNKKTLKLPKKIQQDYVQLYQKYQEVVEKNEQLSQQLYSQILDGSVLSHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0L9</accession>
    <name>A0A8S1N0L9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0760100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0L9"/>
    <dbReference type="OMA" id="NIEQDIW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="2">
        <location>
            <begin position="217"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8086288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="309" mass="36215" checksum="2853A37529BB5DD8" modified="2022-10-12" version="1">MENEYQLIPQSLSILKTNQALRNDYCKQPSFSSFDQINLQLTSMMFRVKINLYYIQDNRLMCRIYNDHHLRMIELCRDDNNHYDCVYSKEFLQHATIVQQIIYEQINNIIGLKADIPKIFKNIEQDIWMNEKVFNDVRSTKKIRSNSEHKKDQQDLLQSELENIQKKQHEEDSTEIQISNLKSNPPGLDNSSIRTNQSSTTPNNKPVILEVPTTRETGKLKFFDEQKSYGFIVLDSDNSDVFVHLDDLQRAGITKEDLRKLGSCFRSQTQVNHYGRGNDKPPSFSFQLMTYVGKYNKSRKAVDLKLINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0P8</accession>
    <name>A0A8S1N0P8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0700004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0P8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="325" mass="38202" checksum="7EC82C40AEA486AD" modified="2022-10-12" version="1">MKIVYQFSTLTTFIYVFLSLPTLYCCPYSDYEDALLPIESPILNSYVNIYMGISFEFGFWSLCVPQQQFKNVPYVDQEFDENQLEDGQFLFLVKGYNGVSVIGFISMSLEPDLVIYNIIINGDNKQKLDFNYHPIIYEGKWILNVFTYDFDKGEISFEMTDQVKQSINVTTMLTIYTQIFLGGRGKIGKLNLNNYKGRLSKILLRNEEFQIGSLFKYMEDNCQVPLPLTEEQKIYLVKGLQIFNGDTSLQYTINQFGTNFFTILIYSFINDLRISIQIITQFNPNSIYNVIYQRISLIQKKYIHFWLINHVYLNQFQSNKYISYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4H3</accession>
    <name>A0A8S1N4H3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0710084</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RING-box family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4H3"/>
    <dbReference type="OMA" id="RQNNRCP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000273">
        <property type="entry name" value="E3 ubiquitin-protein ligase RBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="29"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8083935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="92" mass="11127" checksum="DA392C4FB8C57A66" modified="2022-10-12" version="1">MQQEEKGRFEIKKWYPVAFWSWSLKVEHCAICKNHIMEKCIECEGKDQKEICITQQGKCGHAYHEHCIRQWLKTKNTCPLDNKQWEEEKKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N587</accession>
    <name>A0A8S1N587_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hydroxysteroid dehydrogenase-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0810140</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N587"/>
    <dbReference type="OMA" id="GFSCLWP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09762">
        <property type="entry name" value="HSDL2_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000301">
        <property type="entry name" value="Hydroxysteroid dehydrogenase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051935">
        <property type="entry name" value="HSDL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006133">
        <property type="entry name" value="PRK08278.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808:SF3">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8088537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="313" mass="33993" checksum="1EBE095AEEA8EDD2" modified="2022-10-12" version="1">MQRIQAISNQFNPTIPPPASNVTYPWVMKYPDFFPFEQIGNLKGKTVIISGGSRGIGLAIGIRAAKDGANVVILAKTVDPHPKLPGTIASACAEIEKAGGKALGIQCDIRFEKDIQAAVQKTVETFGGIDIVVNSASAISLTDTESTDMKKYDLMHSINTRGTYLMSKTCIPYLKKSSNPHILNLSPPLNMQSKWFAPHLAYTMAKYGMSMCVLGMSEEFKGSIGVNALWPRTSIATAAVQNVLGGDSMMQASRNVEIMADSAYVILTSCHKKTTGNFFIDDEVLASVGVKDFTKYRCDPKFSDSQLAPDFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5W5</accession>
    <name>A0A8S1N5W5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0760251</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0587 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5W5"/>
    <dbReference type="OMA" id="NWCDYNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008584">
        <property type="entry name" value="CXXC_Zn-binding_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857:SF0">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05907">
        <property type="entry name" value="CXXC_Zn-b_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8086519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="156" mass="17801" checksum="75D21E2E80EBA113" modified="2022-10-12" version="1">MVYYKLLFSCETKNVDAISTGGANYEWHISLVCSSCQTEINEIYFRGDDQVDVVNSRGQCNFQMKCKTCAKMINISLEKFPDTVNVQEISKDIQIALFDCRGAEPKTWNFQGLTVITPNGTVFNDADLTDTWVEYDEKAAEEVLVDKFKAYFQKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N900</accession>
    <name>A0A8S1N900_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tectonic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0770035</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tectonic family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N900"/>
    <dbReference type="OMA" id="NDIMCSA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040354">
        <property type="entry name" value="TCTN1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011677">
        <property type="entry name" value="TCTN1-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057724">
        <property type="entry name" value="TCTN1-3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14611:SF2">
        <property type="entry name" value="TECTONIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14611">
        <property type="entry name" value="TECTONIC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25752">
        <property type="entry name" value="DUF1619_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07773">
        <property type="entry name" value="TCTN_DUF1619"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035802305" description="Tectonic domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Tectonic-1-3 N-terminal" evidence="8">
        <location>
            <begin position="30"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Tectonic-1-3" evidence="7">
        <location>
            <begin position="184"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8086666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="46574" checksum="8D7A006927237C19" modified="2022-10-12" version="1">MYLIFASFIIYVYSQFTKTIQVPVVDENATNTVAENIQIRSTRIYSDPNRGACMCDLIINICDSQCCCDVRCSETFIEEWTILGICLNSNSETDYFYPECGDLVNKQNVFDDKYHFNQINDIMCSAVQRLPYAGVFANIEDTQTLTPDYKSFDIVSTGSILFTLVTNPQYINPTSLLPQGYYSGNPGYIKGKPLMVGKSNGDGFNLIWYKELRVYAWQTNGYCSTGAADYNNKLYKVINFGEDLVLSCSLEATVSLGTYCSSGINYEIMKSFKDNTGSFYYIGKWGSSSNNRTGEWIQINTTQIENVTSSCTLPIEQQLTIIYQKAGFVGDLQNTIISANVTNIPINYASYTGNSVQLTLRVKFIELIDSEIDPNTEKSLRDTIVPYVKKIFLPFTFSMNLFGFGMILVFLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ND59</accession>
    <name>A0A8S1ND59_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone chaperone RTT106/FACT complex subunit SPT16-like middle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0720213</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SSRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ND59"/>
    <dbReference type="OMA" id="QVVTKIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13230">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13231">
        <property type="entry name" value="PH2_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000001">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.220:FF:000004">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000098">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024954">
        <property type="entry name" value="SSRP1_DD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF1">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03531">
        <property type="entry name" value="SSrecog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="12">
        <location>
            <begin position="255"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="350"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="378"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8084694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="411" mass="47836" checksum="A291D0492F05F7D1" modified="2022-10-12" version="1">MADQSIKGTNWGRVIIDDKNLSLSHLSQNVIKFPLKRITNSNIQKNDIVLQLSTEECAENEDMLCEVRFYIPPREQKTEKKKQESNEEEQDEISYLQQVQNQIVKKAKIGGSSDSILTIHEVPLIVPRGKYTMDFFKKDIRFHGNTYQFNTDYKSITRFFLLPMPDEVNLSLVVGLENPIKQGQTAYNYIVMQFRKDLEIQIEMKYTREQLDNIGWKGIRLEYSGSMFDVVCDILSEITGIKVVSPKNFKCKNGLFCLRCSVVPHSGFLFPLEKSLLYIQKPVIYIKHDDIKEIIFQRITQTTQNKFFDIKIVTQNTSHLFSTIDREELDNLSQYFNSKKLEVKKIQEENEGIKNGKEDNEDGSQNGNDHKITLSQMDSDEDDEDFQAQEDSYNSVQFQQKVQKTTRNVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFV2</accession>
    <name>A0A8S1NFV2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0820156</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Neuron projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFV2"/>
    <dbReference type="OMA" id="YMELLFD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14509">
        <property type="entry name" value="PTP_PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000029">
        <property type="entry name" value="Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045101">
        <property type="entry name" value="PTP_PTEN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF60">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE TPTE2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="9">
        <location>
            <begin position="99"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="8">
        <location>
            <begin position="186"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="10">
        <location>
            <begin position="253"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8088951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="46981" checksum="322204DAB830CD49" modified="2022-10-12" version="1">MIQMQDIQESMILNQISSDDEDESESPIEIQQTQKTQHQEQSIIQTVNEISFNRNMDKRINETIVLQNHADEITQKSMKNAKEAYQREIIKELVSKQKKRFKVDGFNLDLTYITDNIIAMGFPAESFEAIYRNPMPEVQKFLNSRHPNNYMVINLCSERKYKHESFYKVAEFPFDDHQAPPFNMMIEFCQKVHEWLTANSNHVVAIHCKAGKGRTGVMVCCYLLYSGKYNSSQDALAYYGLVRTQNKKGVTIPSQIRYVHYFSYGLKNELIKMPFKKIELVSVRLVGVSHGTMIRIQNNDRQLIEKAQIVSEKEILYAFNDIFLQGDVLIQIFHKSIVSESKILQAWFNTNFVSLLPKEQIFKANELDMSKKSMKHSSQIQMELQFDQIIQIRERSNCLSKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFW1</accession>
    <name>A0A8S1NFW1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0850137</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFW1"/>
    <dbReference type="OMA" id="VSQFAIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="8">
        <location>
            <begin position="91"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8090179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="389" mass="43689" checksum="17986041DDCD4F21" modified="2022-10-12" version="1">MSSQQNKQKDVVNKQMIFALNPKPDFNINNPIKINKSNTYDPNYVKNEPSQKTTNQISNSNIGTFKDIEQQKQNNESKCLNKQNKIKYIATRSIAGLKAYTKMLKDNQDSLFYKLEINKQTDKNIFLLADGHGEQGAKVSQFAIKQLSDCIFDMIQSMNTQPKFVEQLNSNITAFFQQIDSNLANLSNIDITDSGSTMNLVLTFENYLISANIGDTRTFYFQRSDKPDQLGPSTLKIKQLSIQHTPEKPQESQRILESGGKIEQETQGFQKTGPLKVKSKKQDINGATISRSFGDALAKQIGIISLPEIHTFQIQQEGFLILATGSILDMIDIIDICRILTPITPPNSQAAIDNAAGQIIEEARKKAKLQPDIQDDYSLILVYIEIDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NMC5</accession>
    <name>A0A8S1NMC5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0850140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NMC5"/>
    <dbReference type="OMA" id="HELEYNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="76"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="320" mass="38099" checksum="E52327E1CA53F7D3" modified="2022-10-12" version="1">MLLQLLSFKNKNLLIRALTNKIHELEYNQKSNQTYVYGNNEDLTVAGQQFLEFYFYDYMLLQGYPNQLSCFKKPNDIIQLRQRLINDQNLAEIGLQLNLQKFLKVGNQIQLKTNQKVLADTIKALIIAQYYDKFQDLESLRNLLYPVMDFLLNKVQQEQIIQAKYNPKSSFIEYLNTIQEEIVVQPKLIIEWKKNEKNYNQSIFQIQLELDISNKIMVENYQLKIQKQGISKRITEQQVYLEALQELKQWLTTYNNNQFQQAEQQAERINLSTSLDSQHINNMNITVYEFPQQIENDTDETEFYDQLSQMLIQIAEKDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NN30</accession>
    <name>A0A8S1NN30_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0960138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NN30"/>
    <dbReference type="OMA" id="ESYEHRH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="33"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="307" mass="36103" checksum="2B874975321BC71E" modified="2022-10-12" version="1">MRLHRNHQGAIQMLKTKIESYEHRHKQQILCQLFPDTPIKWIKPHLDKVIFTSYHLNQTLFSKNQKPTHVYYIIEGEVRLEETQIERRKHQNEMFASTCMAKSRTLLIISAGSLVGEIEALHEKSYQTTAKSNVHYSKILLIPETIYIDLKKQDELYQSTETQHLKDQLILKKINQLKNRLELQDVSPERDLSPQTKISREDFIVQQNKRIQKKRNFEFINLTPEAPLFYVKKIKRVAAPDDKPENRARSISAPTKAKMLSTIKSINSIDKISKPEEITRSRANTINFGEKCRPASRIFWQQIFQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NNU1</accession>
    <name>A0A8S1NNU1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0870192</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="70"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="104"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="138"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8091223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="25748" checksum="51714FF9B4861982" modified="2022-10-12" version="1">MQNQIHQFHQLNKTYKEDQIKHQYIIKKRNLLELIYPLSFTKNRCCLRSIKMVKQNISRRQYVFSCHLVXIGFTNKGVSLQNLKIYEDAISCYDKAISIDPNFAIAYNNKGASLQNLKKYEDAISCYDKAIQIDPNFANAYNNKGDSLQNLKKYEDAISCYDKAISIDPNYAITYKNKGDSLTILKKNKQAIENYELAIKYCKSDENEFKRRIQQIKNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NP99</accession>
    <name>A0A8S1NP99_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0980011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NP99"/>
    <dbReference type="OMA" id="TERCKTM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="422" mass="49929" checksum="D66730BABE8141E8" modified="2022-10-12" version="1">MIIKNQNLIDKVIDVLRYDQEDRRPLQIFLQKHDLLSQHIGLLMPNLKLIYFDLVGQNVDCEKRVYLFLKGKVSIDDILILEGCQILKPTIMLTFSRTLYNQIVQMNRQREIEAKLTFLIQFSFFSGIYLSSIYAILDQIPLKTFQYKTNLSHVLPGIICIIKSGSVVLQSSQGPFHKQFLIITEKDLFGESHFVDEDSKINLKKQNINQKYYEAICNSQVVELYLLNVSELIHTINSQQLRLFLQKKFRQRIMQLRQSIEQNQSFSKPLQQQQQQQQQQQQQQQQKQQQQQSQQRLRTNTQASDSQYEHLKQKETAAIDKVKEVVKNMNFSYSRTRNYFASKLGAGNILFHKAFMYQYTPHYTKKQRQSSFSTAIKQKLSQRTERCKTMSNIEMRTTMLQNSFGKYQFEQKEKQSKHNIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQY7</accession>
    <name>A0A8S1NQY7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0880114</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQY7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="5">
        <location>
            <begin position="124"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8091525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="266" mass="31733" checksum="3A0809D1981689A4" modified="2022-10-12" version="1">MDIKVLFKNKQIKCEICQRKQDQMNICLKCEKPLCDIEKICSTEHLRLNSPEHCVIINIHSNLIACLLCKIEIYDFEQTQQNEEDQYIEETINPQKIRMEYHKFFYKQELRNSELKNQKNVQHAGMLNICNNSHLNCVLQILLNYPVIQQILQKINYEYEKNQYSLKSNRKQILRELSAIASIYSQNQYRIINPSVLIKNLALLDYKIIGYSRKDAYDSFKTIINFLNEEFKFSNLKTYFKNDLIYTKQALGHDWNIMYTLKSSQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQZ0</accession>
    <name>A0A8S1NQZ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0960107</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQZ0"/>
    <dbReference type="OMA" id="FAITEPF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="69"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8094612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="316" mass="37199" checksum="50D98B102B4FE743" modified="2022-10-12" version="1">MGVCHNKKQEQMKTTTILNKSQPRAKIPQTTEQILEFAITEPFEDSNLIYISPQFKHKTQSSKQPSPTYSLNLSNYKYRFSSKYVHQEFKLNEKQLIIHRESGLPYYLEILESNSQNRGLIRKLFNPTSPNNQQDPSNLFNIVEICLQDRLILVVRQYKEAQSLESQLQIVKSNVKTLSILVNQIIKIITILHSLDIIHFNLSIKSFQYQQVSGKIYLNNLSDLYNSEEVNYQYISPEQLNYIPYFTKECNIWSLGMIICQMMDCPRVPIYSDNLNKFKRQVEKWQPKQSIKHIQDKDLQESIIKMLQKDPSQRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NT09</accession>
    <name>A0A8S1NT09_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0920135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NT09"/>
    <dbReference type="OMA" id="GCQKPES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8093126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="178" mass="20741" checksum="D9B96C5F03E30341" modified="2022-10-12" version="1">MQRQQQESSLQQRLGFVQQYHKNHPNIIEFKPLQDYKTQQNLKKPKELEEQPKSSLMDNLNRINNLYKAVSQPRIDAKEVNPPQYFYNYKQGCQKPESNTNKAIKENQIVKTFLNNNELSLKDYDWKPTKGNAFTAKSTSPIDFSMNKSPLNKVTRTDSVLANKKTNEPGVTFLRGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTN1</accession>
    <name>A0A8S1NTN1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1000052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTN1"/>
    <dbReference type="OMA" id="WNNIQNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="67"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="157" mass="18732" checksum="AA0CF0C19DDEBDD6" modified="2022-10-12" version="1">MEQGIEQLRFSNRNRQVGQIVSNGKRKNMKEEEGLKIIRLDKLRQQIVSLEIETKKPKSKCKKLNSENMLLKEKQQLEDDIKDISQRLEKMKQNKIQSRQQQSDLLQDKQDFEQRWNNIQNEDEIQSFQERMQSTLLLIYSQFDISFTQRGLGTTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUV2</accession>
    <name>A0A8S1NUV2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0980032</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NUV2"/>
    <dbReference type="OMA" id="NDRDWEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF271">
        <property type="entry name" value="MYB DNA BINDING PROTEIN_ TRANSCRIPTION FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="121"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="179"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="179"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="2">
        <location>
            <begin position="182"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8095159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="396" mass="47692" checksum="2E86A7CB6F891378" modified="2022-10-12" version="1">MIQVKEHLPQEWFDFIEQIKILNPIDYQTIKQFVKVRVSQFPSDQQMREYYEEVFSKSTNDSNRKKWHQQDKNLLIWCMTKYLMSQNRSDLIPNDRDWEYISKILCVDKQLIELKWISLLHSNLKISTWTKEEDQILTDIASQYYSKNNWTELTIKFNQLSQTQRYPKQIRERWNNVLNPNISKQTWNKEEKIKLIQLILDYGKKWSKIQNLMNNRSENQIKNQYNGIIRNLKRFNVQQTEERSLLKAIIENPDQKLSLTVTQFMSDFLARKEASKRIDTPLLNNRKVEGSSVNKTKVELFPLDNLISVSNISQIEKSKISQESQNQMPQTQNLFHQQNCNFSLPQIQPQQMYYRNQYSMSSLHNSYMNYAPTNQVYQNYYQFPMNLGFQQYQGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NV69</accession>
    <name>A0A8S1NV69_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0930052</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NV69"/>
    <dbReference type="OMA" id="EIMASPW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336:SF3">
        <property type="entry name" value="GUANYLATE CYCLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336">
        <property type="entry name" value="OXYGEN SENSOR HISTIDINE KINASE RESPONSE REGULATOR DEVS/DOSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="7">
        <location>
            <begin position="481"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="174"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8093395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="816" mass="92635" checksum="56F11CE82987310D" modified="2022-10-12" version="1">MTDQPQAQADVDIFQQTMEKNSHRASIFEIVLESTLFQVIINILTLYALFGDDIRVAAFRLSADIVFDALNITCLIMFSSEIILSVIVKEGYFLSFFFWLDTISTISLLLDISLFNQAVGLSGAGGQAKNTAGLARAGRASRVGTKAGRVVRLVRLIRLVKVYKSANKDDEDKVQKLISQRRQKRKANTVEPQIQDRPDQNNPGQSEEGETKESKVSKMLSDSTTKKVIIVVLALLFLIPLFSVDYFVSPPTSMLMQSQQFVKLAESEASTYEDVKNAYDQIIIDHSGIDNYIVLFTSPLYNYKDFPIYEHEDYPNLRTDEQDVGAYTLDYEVLIKTTNLPEAAIANFDKENPDVVGILSTRQAAVMTSILSIIRTIFVSIVLTFGAMMFSKDSNDLALRPLERMIDKINKIANDPQIVTEMAVIQNENSNETVKIENTIVKIGTLLALGFGDAGTEIIRINMKKQGDVDPMLPGIKKVAIYGFCDIRNFTDATEVLQEDVMVFVNNIGDIVHTMVDRYLGAANKNIGDAFLLVWKTRPETYSFEEDNVIWHDKQYIQTLSDCALISFMKIQCKINREPKILAYRQDKRLQARMDNYKVKIGFGLHMGWGIEGAIGSNYKIDASYLSPNVNMASRLEAATKQYGVPILISEQLYEHFSPEFQEYIRHIDRVCVKGSNIPVSLYTIDMNVDNLPPSNDPQVKYKDLTKEELKMLLRQKKLEIKEQVESGEFKVAEHLQENKDLRYLLTGFKKDFMEVFRKAFQAYLDGDWKTARAMFQEALKIKPNDGPTEAVLHSMEETNYQKPGDWKGYRELTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NVG9</accession>
    <name>A0A8S1NVG9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TPL/SMU1 LisH-like dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0930066</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat SMU1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NVG9"/>
    <dbReference type="OMA" id="MMKQQEP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045184">
        <property type="entry name" value="SMU1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054532">
        <property type="entry name" value="TPL_SMU1_LisH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17814">
        <property type="entry name" value="LisH_TPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TPL/SMU1 LisH-like dimerisation" evidence="9">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="204"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="254"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="335"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8093423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="483" mass="55457" checksum="11073BB22B554BFD" modified="2022-10-12" version="1">MEINRNDVIKLILQFLQENGFKKSQEELQKESGICLNLVDSKQNLKKDIIDGKWDQVLNQIKLFNFTGDTLLDLYELIVQELVEVNETQTANQFFLQMIVPNLQEYKERLLKLDYLIRKPGKLSINEIYNDTLSKEQRRNKVGDKVINECIEAPSCRLLELIGDSLRHLNANKIITNNKYDLFMGLGQQEQKVIQISRIEKVVKYSEKMRVNCATFTPDGQNLITGSIDGIIEVWDPSKYSVRNDIEYQVNDQFMMHNNCIINLSANNELLVSLDNNGQVKVWKLKTGKCLKIIESTHPKQIASVLLGKDPQQIYIASDYIQLFALKSGITQKQFRGHSGLIQSMFQSSEGSRLYSGALDQYFKVWDTHSGEQIYSLKFETALEKIYYIQGSILLCPRGKQMILLNSNFQEVKRYNSESELISCVAQGDYVYGLGQGQLYSYEIKSGIGLKLLKLPSENMQDICIGRNVIFCYSQNGHLIFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NVQ2</accession>
    <name>A0A8S1NVQ2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0990123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NVQ2"/>
    <dbReference type="OMA" id="YEMQTEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052993">
        <property type="entry name" value="CFA-57"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="140"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="265"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="459"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="560"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="613" mass="74381" checksum="6CE2C6B709CBB343" modified="2022-10-12" version="1">MQQSEFESKFNEEIEFRDLRYQELKIEKTQQEKQHFNLFRNIENNQFKAVQELETLYENKIMIENEKYLQLEQILIEERQKFQQNLKELEYDCQKQIKEIKQKFQSDLKLLYDAIISNKSDIKILQNNYQSIIQDDEIQDEYIENTKQQFKQKIDDLQKEISSKDKQLMMYKSEKFNCKEIDVVQQQLELEILKQKESEKFIQELQSQNKELMEKINLHEQELSHCNDQIKQYQKYIGGLQKSKLVLSFKTYEMQTEMEPKDKKIRELLESIPKLEDEINELSTKLKQYEEKDKRMINQMKVMNEDIDKLKQNCKKYQDLSKQITMDIYNCYHEKKEKEWVDFMKQMYQKYISKNEKNDIRNPENITEQDKKTEIAIKLFIKDITSMIHEFRETDWPQQLKQLYQKHFTGEITNELCRQNQFLVKSSQENDKKFKKLLLWRDQEIYLKTQENMHLLTHLNQVLERKKSLETKLAKQISQYESLLKTQTRASSMQQYEQRRRSHQQENSAILQDEANLQPLNQSVDLVNGYITNRFPKKGKIIKGQNYNKQNLSRFEQHRQSDLVTQIEILQTKNQLLEQQIKTLKFKLIEAGIDEEVTQTNTQRPYSQKVKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWU4</accession>
    <name>A0A8S1NWU4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Adenylosuccinate lyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0940157</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWU4"/>
    <dbReference type="OMA" id="FNDLENH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006188">
        <property type="term" value="P:IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047136">
        <property type="entry name" value="PurB_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013539">
        <property type="entry name" value="PurB_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006764">
        <property type="entry name" value="PRK09285.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43411">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43411:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08328">
        <property type="entry name" value="ASL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="4">
        <location>
            <begin position="27"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Adenylosuccinate lyase PurB C-terminal" evidence="5">
        <location>
            <begin position="347"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8093985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="54469" checksum="401546236FC4B167" modified="2022-10-12" version="1">MLIFRRLCKFSSQFKESTLTAISPLDGRYASQTAVIKDYFSEYALMKYRIKVEIEWLKFLHSKNMIKQGNSVLNLTALDLTYLDLIYDKFDVTKSFRVKQIESTTNHDVKSIEYYIKEELDKNPILHSMKEYVHFCCTSEDINNIAYSLMMTDAKNNLLMKSLEGVINKLVQLSHDHYNVPMLSRTHGQVASPTTVGKEFANFAHRIRNHSELLRNLKFEAKLNGAVGNYNAHLLAYPNYDWPILSKQFIEELKLKHNPFTTQIEPHDSVALYYSYLNLINNILIGLSRDVWSYISINYFEQKSIKSEVGSSTMPHKVNPIDFENCEGNLGLSNSLAQHFMNKLVISRYQRDLSDSTVMRNHGVCLGYAVVGYRSLIKGLDKISPNYDTILNDLENHWEVLAEPIQQIMRQYGVPNPYEQLKELTRGQKITKDSLREFISKLALPEDVKKKLMTLEPKDYIGNADKMARLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYC0</accession>
    <name>A0A8S1NYC0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0900150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYC0"/>
    <dbReference type="OMA" id="KQCKLRD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="178" mass="21071" checksum="DBDD611E8993805F" modified="2022-10-12" version="1">MFLFQKRLMTLSQSSQQPIRLKPKFYSQQKENIPVQKDLPKVNLINKLNEASKILKPLPKQQIQINQKTISKDVWHKKSISMKMKNANFKDGKRIVSGQLIKYKKGDQIYAFRCIKDTMKQCKLRDIKLDHDVDTDDEQIFMATKNMFMSLCQSIKRELFNNEDNQIENKIIIDDDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYY7</accession>
    <name>A0A8S1NYY7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0970131</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYY7"/>
    <dbReference type="OMA" id="AINENCD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="637"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="190"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="640"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8094985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="676" mass="78194" checksum="DE98902ACC8254EF" modified="2022-10-12" version="1">MDSQSPKVNSLSYHTNTKMDITTIFEKYKKTHNKLSPLPLNQFYPMAINENCDLLQMQPDSDVQNFVSFRELNQGAVSELKRSLQQSKMVWNNYSKYLFGDPSLDEIEMDSVDQQQFYTSRKLQLIHNRETFHKMRKQYKQQEEDQINQVKQALKKIHSQTNGIKVYPSLAYQDQQKLELLRKQVRLGGVQQLQERNSDAQQLQKRILESQVALNQIKKQFAYAYNYDYNFWGPCSRDGSSMVTFDRKLYLYGGSSSVQQEDLCVAIVDKQFYKWSPQKLDEKSIHGYRVHHTAGIYKNFMILFGGEIHIEQSNHKLISECTSDIKTISLTSFDIKLIKQPGTIPPRKCHIGEIIGRSLLIFGGIDNRGNYMKDLLCYDISQSKWVSLNVDPFDLYANGIAFHKSCLVTQNKFCDIYRQDPDLKFSNQGIYVFGGQDKFGNYLDTFIRIDVYQKPIRIEQVDYKGSGPIARCQHSMNYNEVLSAIVVYGGKNDDNTIEGFLNDLFLFDVKNSSWIQLELKGAQMPGRCGHSSSCIDTKIFIFGGYNYNGFVKSDVLVIELDSNISHQLIQSDKINEDKPRNSKIRVSFHQLPEKRKTIDKTNERKKEIEKLNFKNSHSFLPMPRRVTMLINAMRFGQEPKSNTEGSGSNSNKNSPKDGTRRPHRNMTMIIEGKQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1E6</accession>
    <name>A0A8S1P1E6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1010155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1E6"/>
    <dbReference type="OMA" id="SSRCWTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="224" mass="25535" checksum="EF03C6A8229E39BF" modified="2022-10-12" version="1">MGSVCQKGDNIVNWPCRSNPRGSNPRTTFTIIPEEDSVIYSQQDRSVFTASQFYQNPDSQRQTVILGNAIGIDNKLQILNVYLSKLLSSHSIKLTNTRSNSYEVSLTNLNPELCCYCEWHLEADKLPIRSGDKIDLIHVLTGRYLMATELDEEGVVFCGYGSSRCWTIEFDDNILKDGAIFELKHNEITKYLSFINKKASLSHNRQVCLNDNEGKNNYWKLVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1P7</accession>
    <name>A0A8S1P1P7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1020115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1P7"/>
    <dbReference type="OMA" id="YRKIKIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="507" mass="59083" checksum="8ABA47BFAE0C8703" modified="2022-10-12" version="1">MKSDDGLILIQKYTSEDDCLFCQKYGRLPIIKINFAPLLNRALYTKYSQSSWKGKKFEDFFSTKSIKWVIQFKDKLTYDDQDEFLKRYYPKSDQQNKLDQLLEYYKYHKDIPRMFLPKLSDLAIYFYEKKKQLEYRKIKIMLGIPIDDASNYTECEKLKEEIKVLNSITQQTQVSSLSLLRDMLKQKGSEEIVNIDATWITMANHQQTKSSQSSNLKLLKQLISKNTQFYKHKLNTNYTGLSKRNTQNSPQQNIHLKTLSKDTSQKSLLSSHKLINNSNGDSEFQKFLKQQMVGINHSNPCNQNNVKSTTKPIQISNYQSKQNSIHHLISTRSISSEQLKLIQSQQILKPNHHTNNINQQSQHKKSQTQTHYTEINSPNKHNKQASNKLYTNIGLDFKQALTHTKSTDKLFKVYTSQSSIPLSTNINININQLNMNNLNIKSSFQQYKAMLESPKVNPKKKTQSNVETQRIHNASTQKSTTSFKKSQISVQQIYAQKIKQKHKIQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P202</accession>
    <name>A0A8S1P202_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1030043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P202"/>
    <dbReference type="OMA" id="FGVNQKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="473" mass="55435" checksum="4DC87AF723727F44" modified="2022-10-12" version="1">MNSLNNGLNISDVLRNMPSYTFTKTMELIESIVKQIEYDKIKSELNEECEPEKPIYDQWRRQNAQLMKEFIRKPDNKMEELVEEQFEEKPPSWEEFQPEIVQTQQEPEGDLYDDQLRSQLSYRERMRKEFEENKAQIEKEKQEERRKIRVLVDGQPDKVTYDIDGKIILKAKRVQLAPVRKIEGQQIKESAPSQTDELAVIKPKRPAFLKPKLKVFKVQPINENKEAQKIIAEQERNHLQEFMDVLELKPGVFLEIEHYEKGLRNKHTQAQDLPYRIQNKMDQYLASEQSQSIRLSKEEYSSITLNNSILRGSYEQSYLKKKSGIRQNSTQKTKQSDYEDTIPKNGIVKVNDIGKFYDLLIKDSEIIEENTQTEFPPILMDRQESLSIQEFKTPIKSELPSIPATQFYKYLSKQQPPSLSRIDNNSVTGSVTSKLTRDRSMPEVINNMKLHQCLAAPKIGKFLGFGVNQKYKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCY8</accession>
    <name>A0A8S1PCY8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1140082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8101046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCY8"/>
    <dbReference type="OMA" id="ESQEFQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="527"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="578"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="191"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="334"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="536"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="584"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8101046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="615" mass="71345" checksum="03609530B44FD533" modified="2022-10-12" version="1">MQPLFKQRKPANLIIQSSDEDKIVQQQIQELDTFEEFCSNIPDVENQELNFTKESKSFSSQPIDLQESQEFQYNLKFSDQKQEEIQESPPIQYIEFVEFNPQSINSGQSESNIQRIDSQQSPKDIMQEPPAPQNKNLTKFSFPQKGGCFSSLKKQLCVDQPLISNHGNQSEQFEETLVKQHNTQNIPKSVQDDLLKQIKQLTDQKNDIQTTLQNQIQQLQTQNQKLEQELVQQKQQNAIIIQENIMLQSKIQILEQKKSADLNNITDFSHDLLPNKLSMNEKLIYLLSKINIKAQENAKLQYEILKLQNNIEILETQKYLQINTNLLLETHKLQQSHSPQLQPQSQNRGQSLSKDSRTSLLNSQKYPLNLSLNYVYQGERRQEVFINNKNRIISPLAHYSSVGEQQQLQLTNLQKNKIYKEDTNYNNKMHTKNSTSVGELISFKQDLTNCSNMINKALRKESYSTQSPNSKVTQKFKQTLNAKIRTQQENSKSYASLLFSLGERQKDVRQMSPKGGNKKSKQLFGFIMQPHPNSRKQSEILKSEESNKMSQEQLKMSDKKSTLQTSIDIFQIMREMRNKKNRISTKQSQKKGQATTSRQLITEIKSSRTNKPSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PI56</accession>
    <name>A0A8S1PI56_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1190144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PI56"/>
    <dbReference type="OMA" id="RANNWSV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41747">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_128, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41747:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_128, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="381" mass="45176" checksum="EE61905F91730710" modified="2022-10-12" version="1">MDVPINNFKGVMLCNRPNENVMIIKEKPFCSRVQPYDQWGLTKKYEETKVLPAPMNPVLERHKKWLEEFKLQNQLKKHAKEEQQVREDEKFERVRELAKKDREITKKMKEEYKQINDIIKKELQDDGPKSKTVQLTAENLKKLEERDTIKQQEVKTDNKLDIKQGDHKKRYKTKPVWAMTKEEEEEHIKQEEDELLNFVENLDYDSYINDLEVNVMLKALQQRVSDIKKEGNWKEKLEQGEKKQEQRELDIYDKISQSLGKNDEARSVHSEKTQNSINQLRKRQELIEQGKQDWEKTTTNGEQKASLEDRIAKHVADEILNQYKNISNVHSNSSIRKILEREAKKTLQEQSIPGPVISVIKNEKMPRDPNTLPYLHRNPAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMQ6</accession>
    <name>A0A8S1PMQ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1240071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMQ6"/>
    <dbReference type="OMA" id="CIKLNCD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="115" mass="13453" checksum="E89AEC09283735E6" modified="2022-10-12" version="1">MQCSSPEEASMCKSLCCNKFHQCSQYQSECSIFSDNHCIKLNCDTCCMLQDNQHFCGTYVQCSKYFDLFYFWCLIILGLLLVVLLIIKLRTKFMTARMIRIHQAFAKFNKKTTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSS8</accession>
    <name>A0A8S1PSS8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1280050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSS8"/>
    <dbReference type="OMA" id="LTQYNCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="237" mass="28763" checksum="46B2F286FB66141F" modified="2022-10-12" version="1">MNEICLTQNHHIFQILYQYLQKHGMNIGNSQMTKLFELFIQTYESTYQRKEIRISQLNSKIFQITQDLNNYQNNIERQYSVIKNIKNKREKLHFDLDFKESHLHQIEISINQIKTYPSITQNICDLKEGIRICRERYLLLKEENEEFNKIQTSLTQQKQQLKNRYKFVLDQIQTNLKFYDEFKENSINLNQRLIKQLKTVIELFQLNQKRTINQKYTNIDKNTIDIDDTSILFINLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q006</accession>
    <name>A0A8S1Q006_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1360010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q006"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="65" mass="7651" checksum="48712AE563568730" modified="2022-10-12" version="1">MENDSEFLFNKKVMRKMKYMVSVSIIIVKKIHNFLQNEIGISTAVMKSIKLKIIDPTRINKNHPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q340</accession>
    <name>A0A8S1Q340_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1410055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q340"/>
    <dbReference type="OMA" id="ANEITYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="140"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="430"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="668"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8109574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="919" mass="109869" checksum="C942D4447B89B611" modified="2022-10-12" version="1">MNNQGDDIQLLKKTINEKDQQISDLEKKLRLEQFRVKKQQNELNAKIENEKSTAQNNADQINRFELQINELKSKLSETEKKNLQLIEEISTLKKRRASGSTDEDFQPKKENQGDFPSLQQILGIIQTNQGNYQPELSKLYECYTEACQSKDEQINSLKEEIKDLTQQLDIKQSELTKQMEQQEERIKQLKQSLDERENELKNAVQQTSDNSDQKIQELEKQYQSQLSEKDKQLQEVTKKAKFDGFKLNKQLTEQKTLIENEQAKTNLLQQQINRHESSLRELEIKLSELEQKNLILIEEVKQKQQEIEKLNEDSLTRVEEISLDKPSYQELVDLIQKFQANEITYQQELKNFEEELHLKNTHSFELQEELNQYIEKFQLQDRKYQESLRSIKALEEQLSSDRQISQELQHYVEQLKQEKEYQQSEFLHKIKELSEHNTNIDDAVNDVEQKYQKMMMIQEEQYQRREQELIHQIEQTEEKLSQQEQLLKKQEQKYKNEIKQSEENNNQLIQQKQQQISQLQKQLQDDTHKIKEETNKQINEIKQENNKKEKSLEQKIQEVTKQYEGELNKIRKMEIILKKKEREIAEEKQGKQFAEEQLQILTDKCNKMIEQYDNKIREIQIANQVNKQDQHRHSIFDQRMSISSSDINKTFEIEVLKAQVEQLQSKGDIELQAEINRLNEKERALKKELVDLQNEIGNERLNFTVVQRQLEQEVESLHQEINNLKSDHQNELDEINDQNNKILSSQKNEFQSKINKLEQKLEQQTNQFNKKQNDIEEEKENIIQQLKIKIKQFEEQIIELKDKKNFKNPTQQTIIQDDTIQLQQEINILNEKERVLRKEILDLQNELGNQKLSQTIKIRELETLAEQYKQESIHAQQEQDYAEQLTAKISIQYADLAMEYEKAVLQNRSYRKSFSKLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q3Q4</accession>
    <name>A0A8S1Q3Q4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1400078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q3Q4"/>
    <dbReference type="OMA" id="HEMNTVP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="86"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="308"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="381"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8109308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="872" mass="104531" checksum="3A959652BFEA8220" modified="2022-10-12" version="1">MSQTRTSQSSKHQRKSENRFQQQINSLLQNVSNPPLNISKRWIKDREGEQKNLFEEVKQNEFTGKNQTLTQLTEIGKKLAKMKKKLRTLTADDRLKTPVSDNKSRKSTKSKKSQSKKGQSSHSQSRTQLKSLEQSRSKSSKKVKKQQKSSKTQNTPKFKKKTYDNKLGSFSKEMQQQRDSSRPNVRILESRIAKNTIDRSSSPSRSIGSSKKQQRNRRVETPCEESSSLTDSSDTKEIMKLKMNPINPQSKYEVFMDNEKKIKELDAGQLSREENLMLTTKLESVTKEKLLREYRILYYRSKEVKKLLTEYYEQNLHLTEEINELQQIIQEKQLENKQVKKELKSQCKEMKIMQSEYSTKLDQLGKSYQQHEMNTVPLNDYERLKQENDKILIENEMLKEDYKIISQKMQQIENEIYQKRQYEIPEQEMSQRLQRELESVRDQADRIKEENMKLKLQLDKEQISNRELSQQINQKKNELDGNVEQKQYELSSLKSHLQTANTKIQKLEQQITQMKYQMISEQEQSFSIREKIIRQEEDLRIANLKVENREKEINEQRSKELILKKKILDLESKIENQQQQQSQPYFEPIITINKPNNSESFINKELQMERQLLAKTQEQLQNIQGENDQLKRNLKQIELELQLERQQNEQIKLKFSQLQQENEFTLQRAQKQFEETENHRSKEFQLTEQQYKNQENLLFSAQQRINELENEFRILTNKEQENKNQINDQKLQIEKQQLKLEQNQTKINEFQNQIKTLENENAQLQENNRQLKIQNQQLIKELEQIDRIKDNYKNKYLNNRYENKKLLNQYHEKENIMKQTFTSELEKVKCQKEQLEKVKNQEECQRQQKDKKLKVINDLQQMIKGHKMNLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4I3</accession>
    <name>A0A8S1Q4I3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1430029</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000147">
        <property type="protein sequence ID" value="CAD8110127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q4I3"/>
    <dbReference type="OMA" id="FIRIIKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="476"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8110127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="884" mass="104770" checksum="AA06D0E318961B49" modified="2022-10-12" version="1">MNSELYSNRELLQEMKKQDSMRSQRQLKSCPAYFKTLQFQKYQNIQTSEQNQQFSKANPLNVFIRIIKFITLITQSVFPQDFKYLDSNMFKIIGDKAADFGFYVKNDYFKYIKAAPESRLQHILQITILKYYTIQSLMEYLSHSKFLFKPEQTYILIWNVYLLIMINFNFLYCSIKFSFDFDGREPEYYKEGEIFFLIIPFISYLIDILVKLNSCYYEAGYLVTDRHKIIKHFYHSNECIIDSIIIIISLGYFFDSKGNNLLSLFMIIKILDVPKRVGLILDKLELTTNYWAVYDLIRLIYLIIIEAHIFCCFLYYVGKQNKNVSWIINEDLVEANFITSYLTTFYWSVITMTTIGYGDFTPQNLNERVLIIFVAIVSCCTFGFFVSSIGQIIYSIQKKEQQIRLDLNDLKKYLRVRGFNSELQIKIRRYFEYLWNDCMGQEQFDMSKLQQLLPSNLYTEMILDLNMKSISKIPFFHDNFSSDFLQALAGDFEEEKLTPWQSIFTKGEQSQHLYILCEGEVEYYVSLPEGSGNCVSIQKMDGQDEIFGQQDFLLDQNYSINCRTTKPSRILKMHRDKFQQIAKKFGYEKYCQLKDLVKFSGRFDEFHIQCIGCNKSTHLLYNCPMLTGFPSRTKTLLKYKKKYCQERKVFKRTNLEKRISTLSLEGKIADSVLYYLLKDPKMSEQFNYQIKRNMSNSQNGNLRKSQKKITSLPKRGVPLFLSQEQYSRTVETNFEENLGFQFSKGVIQQQKKMTLLDQELSLDDSSKIFAALNKQFLTKGTSSINIVKNNQIIQEIEKEYDSKDEKTLLIESKNYQESMGRYKDIFESFEIQRDYELYMPHMNFKLIQEMFQKSREIASFHNFIDVNIVKRKWKRSKIVIVLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7R7</accession>
    <name>A0A8S1Q7R7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1470052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7R7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="679"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="743" mass="87611" checksum="0DBD42FB00CCF0F7" modified="2022-10-12" version="1">MSNFQFKYYPSKALYIGENQEEGNSARVDQTVIWEQSSRLSEETQAEKKEKVEELKVEKINYAEGIRTLKLFDNRIQDIDDIRMSFSESDEAQHSSVFQKNSDNPDEIEQGQRNNVFRNRKALNSTINDYQRPKVIVYLSWTINILMITVLTLSFTSYFLGLFLFENVQNSLNLIEYASLRNQECSQIVMNVQNLEMLRIGIWNMTESEAIIYEAEQRTELNNSIFALTDANKKIMLDELYINEQIEELHSKSVVNVRISKNSFSNYDLIEATQQIISKALLVRDKPLQNLTLDDEDATFITYNLQNAIIFQYRNETNQYSYGIQNLTENNVNIFFIFMIVAACSFFIQLIMIVILIIQINQIQEKILQLFMEIPEKTVKYLYNKSENFISNLQVGEEEEVSSDLSDEEQDQHKELSRTLNSKRKKKIFKNTNSFHRSQILIITFILFTFQGYFLLNYFLNQITYNNLKQQIPELNVTARCGSYYRFVDNCERQLFLNPEEPILLENAYSVVMNNIQLNYEVDSNLHQEHSKNSEIQNSNYYDTFQSIFMFNPCNTFVQEGYTTIEYCETFANGSIQQGMAVAIARYFENVRYIMTIYDMFYGHPEVNFSVAARGWGRFRNITNDSDNVTNYIYNLNNFKQTTESRIMQNVFLKVAFRYLLDQFLTALKQDIEITQTQLLAIFIVFEVLLFFIYFIIWLPAQMKMTRDIWRTKGLIMMIPLRVIQKIKTIKEFISFLVHSQDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9D5</accession>
    <name>A0A8S1Q9D5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1500064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NIHIVHS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000129">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057352">
        <property type="entry name" value="TPR_TmcB/C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25474">
        <property type="entry name" value="TPR_TmcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1233"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1335"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1577"/>
            <end position="1600"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="650"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8112158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1643" mass="191291" checksum="E5FB199BBA018898" modified="2022-10-12" version="1">MELKSNFGTSKSSLDSDRSILDKLQTEMKGIFFRVIQLMLKDEEQSIPFTCIGIVIQYMQITYIIFNRQIWKVWQNEIITKQLNNIFGYVLLTPYFEMVSFSGFVAMMYVCLGLLLFAMMLIFLLSYSISKSKSSFTWPIMLLRFLMSLFLQVFYMPIIDLLFSMLSCNTNQNGVLMHVLFDFECWVNIHIVHSVVAIFGVIFFVILCLHFSLLYFEPRYQPLEASSKLSGRANAVFLIYQLIMVICYTFMNGRNYDYLMILIMLIGSFIIFWKIHIEQPFNNLYIQKAWSMLVALNLWGVILICFAKFLEGVLFIGTVYAWIAGLPLMIAAIIKSEKLHYDLLLLNLNKVQDPDQIVQLTNYLLKLLYKSPLNSNCSLLIDGFLEIHKATCTREDCYLKQKKMINQRQQKPLFKEGTIIERDIDLLMVMAQIYFNQIKKFPNEINLRIRYAFFLLDNMKQRQQAINELIQAETLSPSLDNDFVIFRYKKIIEEEMNAAQNETLGNLDVATEIAFQNNMRSFQNKIERATLMHMDFWSQLQEDSPDLGKMNDIGSKINLAITQVEELWNKMQKMTQNLPKAMRLYAKFIIEVLQDKEYGESLLDKSKLLQTQNNKMKNKQSIQLISSDEIGYESQPILLVSAAQEKFAQIINLNLAACNLFGYNKSEMINRKINIFMPNLYSKFHDAYVENFLQSNDNKNINKDRLIYIKMKSNYIMPCYIYLKIIQSLDENVSLAAQFRVLKSFKPTCYLILDNEEIIDSISSSCIPLLNLDSKQISHKKTSISEIFPNYLQQKQIFLSKTGGQILFNLATSSAVSNSQLASQEFDEGDKKGECIQFQCFTSEVFNELGDQVVGYIVKLEQQIQDISMNLEINQPQQKVLTNSMQFKFNPSKFLYQGEFVSDSNSQRVDQTILWDQNDQSSMISSVQPGVDLIKSQIKSERSDDGNIQIQSKINYAEGIKIVRLFENRVQDIDDKDDMISEEDDQQGRSVFQRQQDVDNDQDGQIQDFNNIFKSRKNLSLIVNNQITPKVIKQLSWTANFIILLLVALSFSDFFVCQAQYDEIYQTLELVEFTNLRTAELHSILTSVQNLQMLNLNIFQINSTEAIIEFEKTQKTKLNNSIQLVSQLNKDLMLSNIFISDQLNELQSKDVVKMKFGEQYQNYDLMEATEQIVSKALNIRDKQLSKITYEDDDVEFIIYNLLNDLVFQMRNSSNLYANGLTIKTSEKKETFQIILGISAGALGLGLLMLTIITLSVNKIQEEILSMFLDIPDKTVKYLYNKSENFISNLQLGEDDDVLSDLEELEKEEQEELNKTLKSKRKKKKFKNTNKEQRNYIFAFFFVILIIQAYFIFNYFMSDTLLSNLAQLVPEINATSRAEGFYRFSDNAQRSLYINRNITIENDDSYKKVKENIDKLYALDSSIHQEHSLNVDITNSIYTDAFKELFMLQPCTILSSYLEDLEEPDCQAFADGAIYQGMAVGMARYFENLRYIMTIYDQFWNNTKANFTVIARGFTTFKNITKNSDNISNFILNLNNFNQSKESRVIQEVYNRGTFRYLMNKMQISIGKDIEDAKTQRLALFIVFEVLLFVIYFILWLPLVLKLTRDVWRTRSMIMMIPLRVIQKIRSIKQFIKDFLHTKELEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QEK2</accession>
    <name>A0A8S1QEK2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1550036</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="QWIYKES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046851">
        <property type="entry name" value="NBCH_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF112">
        <property type="entry name" value="BEACH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20426">
        <property type="entry name" value="NBCH_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="4">
        <location>
            <begin position="1422"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="3">
        <location>
            <begin position="1549"/>
            <end position="1845"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1993"/>
            <end position="2034"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="682"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="789"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="682"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="817"/>
            <end position="833"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8113391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2211" mass="257323" checksum="920074933913BEA7" modified="2022-10-12" version="1">MIIDYVKYIIMFQDKYGKTVSEFNQKYNIVANPFQLRFEEGLLNYKYNSLLGQFQDCLLQDIRAAIMTAQRGYLSRKVLKHYFKELPQQLRAEYYGSLLTLYKESLRQQQQNMFYFSGMGSGMKVEKVTWPFVEGFGFCIWIQVENINYELFNEQNMGVQKIISVHGQGQQGGGGLECFILRGSVYYRIIPAIYQEPDNGAILIGQLKNGMNFIGISHECQKKFTSSHLTLYFNNEQSKTMTLDFPRLNQTIALTRFTICENLFGTAQCIIIMNQSSTKIKTIQSVYGQLQMDFKQLKTLPACLDKYFERIMSIYIPEFTDGNHVIDVMGNCNATLLTKSGAVMADKNKFHFSGGFKLYYVMLHLSGTLYKQINQAQLNLSIMFEVINHILKNNHQLQAEAFNSQFFLTVASIIISFERPLINTKCVELLIEIKSIIGDNRLLDHFFIHILWNPKLHQKCISDQVFSKYLQLACQVYLQNPQHYQHFISSKDILDMLVFAYTENQCCDYHFNDYEESFQMQFLEKIFYLFNQQSDISEHLILFDQYLLSKISPCLLLQIFTILKSLLVDNEDQGIKTNFILQTWDQNNIVDSLLFIFKSTPYYDIRAMIIYFLHLIFQQQFPNHKLQTHQAIEYITNAIEPLIELSRSSIVQPIKKEQLQQQQQGDFDELKNLGTQFLNNDYESVPQAPSMTNRRKPPQGFFDNQQRPPPRAQSIPAPETEKSKSPSFSVDQKGKKKFHIKIDTDAINALYNFGGEQGKKNAFDHEAFNKELAEINRLAKACHMYMQGHREPEEPYVESPKQQEQFVESPKQQQQQTVVRQPPMSTKNSQAPKQQFQQELTQIKEVSSSSMSIQQVEQVQKKKIQSFASVYNQLMSWMIDKLKIGENESIMIEERDQIKNEGIINAIMKIVAFSIEDNLKAQILQDLLCLCKSHQGNSILILGQATFQNELLKILSSQEDQNLIYEIGSRLHTTLIVQVLKSEFIGANYVYELLKWGVDCNQKLVELQMNVIIDQLYDLCKNFQTDSLLWKNIIQTALYLIELKQHYPQLIKKFNKFYDLIHKDYIINGNTEEQIDQIQKCFNHPELAQKVQSFFNTKIQNHQIYEIIIESTHQDNLQQLLNTYAITLEICQSEISYGLAHILSKCRNQQNFKEFIKFLCYIIFVSKERNQPSLLRKFMIDLYQEFETVLESLLAKSFQDDSILDQLIDQISNSNTLKKEIQIKVNIPQQWSMESIVSPQKVSRDVQQLGENRYIEIEERKSQWIYKESEKERIGRNKYHKSLNSLFLNDGWLSQGQGCIQRNDLVQDIIQEEFVKNKYFNIKISKMITKSFARPYIKSIPIIPDILRNKPVYQTPQTAPIILGHEIDKFEQLYGQRQSTNSQAVQAATQLLNMGKAFGNNIKNAVKTIANQKIQAIDNFKQFGENTFKGKKVLVINGLYVYYALMSLTNTHILLQYEFLRSEGSHAALGVFELRDDQKLIKKYSIYNLVLIIKKKYLQKRTALEIFFIDGKSLFINICDQNDLDEISSKLLNYRKSHQAPYFNQSKTTDPVKLLEKQGYLQKWNKQQMSNFKYLLLLNNLASRSYNDLTQYPVFPWVICEPKDVQIEQEPSVKIYPEGQFRAMQKTMGALGNNQRIKSYIERFEQSEQGGEIPAFHYGSHYSSLAITSQFLIRLEPFTTIAKEIQGNKFDIPDRLFYSLVESFRCATEDIADVRELIPEMFCLPEMFLNLQNLQFGKTQNGIVVNNVQLPKWANNNPWRFVTGQRCALESEEIQRNLPEWIDLIFGFKQRGKEAEKNLNIYFYVTYDQQLTLQMMEENRLSIEAQVVNFGITPLQLFLKPHIGRQINTEHHFVSNSQELKVYRPQNKKKVPNTMKPIIDLQQASNRAIVKIKWISDVRLICIRKEGKIDYLKWSSQTDVQANQPPFQCGLEREKQFNFEKPQTELFNIWDVSAQLSSYPLLVFNQGKLFICGGYHLGKLIIIGDNNQIIDIYQIHTSTITTLASDKKESIIISGDKSGHVILWNVDKQKDIYKLHAKCMYFDHQNQINCIYVSTSMKLFATGCTGGYIYLYNLYNGQLMRSFIHPNKNPINSIIMSNRPLFCIVFYSAYDHQLYSYSINGFLLEVQQEQSSSLIDCQIMRNNLFSDIMVYGTENGELVRRSLPYLQQLKRFQISAKSPVLSISCSKDKKFFICGCNDGEISVMAEPQQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QH03</accession>
    <name>A0A8S1QH03_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1630079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000170">
        <property type="protein sequence ID" value="CAD8115288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QH03"/>
    <dbReference type="OMA" id="YYNSDLQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8115288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="80" mass="9515" checksum="C1202A4894C3DAD7" modified="2022-10-12" version="1">MYQLWRFIKRGIVSQPHMVGGVIIGYFGIGIISYYNSDLQFNYGEYNEPHSHNHGEQVRQYCESTLLKIRSQINELREQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJM2</accession>
    <name>A0A8S1QJM2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1650049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJM2"/>
    <dbReference type="OMA" id="EIVIMND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8115586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="313" mass="37016" checksum="3896F356EA4A0453" modified="2022-10-12" version="1">MDPFIKIQTPQKSMIRIILDSLIVFVKLPLFAILILEIVIMNDYIFRQIQRLQFHIVFHKFLRIIILVHNYISGRLLLLVLGYFKYKSLFHNNTSNFKHVNLQSYCFYCLRTSPIDVLVYITYFSPSFTHISIQKDKVKCKIISYGTAIIESFYISKCSFKDFTDGENIKDLMIQIRKTRTGPLIVFWEGGITNGYSFLKIDQTIINEIYNINSFAKEPLQKSHYLNQNPQGIVCLQNDDRMNLIGNSPIQMLITILKNQFCSLEMFFLKFQPYNFIDHLPDIYQGFIEGRIKRGISDYSWIHYQQQFIQQNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QLN2</accession>
    <name>A0A8S1QLN2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1650021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QLN2"/>
    <dbReference type="OMA" id="DCIRTQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8115530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="51" mass="5909" checksum="0052239937B60AB6" modified="2022-10-12" version="1">MISETQFRRDLNVKFSLTGLVHDLSEYGIQQGSSQQDFSMKLSDCIRTQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QN23</accession>
    <name>A0A8S1QN23_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1670030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000176">
        <property type="protein sequence ID" value="CAD8115937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QN23"/>
    <dbReference type="OMA" id="IITHYSA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8115937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="137" mass="16150" checksum="3C49D0AE5A6562D2" modified="2022-10-12" version="1">MKNHRLILIMNQYHHSYSPIIKQKHRSIVQKLRLKSITVDARKTFVLSQPNSKKQILSPLVKKQSEQLNFCINGKGQIESNQPQKILRRRNNNSEYQAAIITHYSANSFTYRHKINDAGVESMFCIKKYQLLRKQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPN7</accession>
    <name>A0A8S1QPN7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1810013</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000190">
        <property type="protein sequence ID" value="CAD8116795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="65"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="130"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8116795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="246" mass="28189" checksum="BAE67E133F956D5A" modified="2022-10-12" version="1" fragment="single">MRVQFITCISLDEGQSLLQKILKFQHKCDQFEWSIIIEFLMEQFKNPLIKQIKWTYQFSQFCLFFSPDGNTLAYSSSDKSIGLWDVKTGNLIAKLNGHTSIVYSLCFSPDGYMLASFLWDIKSGQQKEILDGHLNAVVSVCYSPDGGTLASGSFNKSNRLWEIKIQQQTAEQNRHANTVQLVCYSHEENLLAFVSDDNFVQLSDAITGQEIEQSKKNYKEILAKFEKLLSQKIFQVANYCKNPCYX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQB3</accession>
    <name>A0A8S1QQB3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1820001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000191">
        <property type="protein sequence ID" value="CAD8116817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQB3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="210" mass="24832" checksum="D7E3C323DA152278" modified="2022-10-12" version="1">MLLSTDLFFTRGQHELKEQLWPSPIFQVQSLEQKGHMHRILLHFGSIQFKLYEMMNMNLQNFLMNISCSTKDLKSIIHKELSNCLVINDNDCQVFSKLAFCQESIQSSLPDNSYLNTTSSSLYYKSYSFHRFCPMDEVLWINHYLNWLSISCLHPQIYYLIPEVALQTITTCYLNIYSLKKLRYVVRIRSLVRRSLYGLIKSISPFFIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN03</accession>
    <name>A0A8S1JN03_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JN03"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="122" mass="15041" checksum="FD631EA516787F21" modified="2022-10-12" version="1">MRQVNQVNQKKKQNNQNIHLRIIRLQEIFYNNVKLVKSIFQEALNKKIIKNTHLKVWVIRSFYQQYIFSQKKMYQYEELLLKLRVNSINKPQRKSFGEFILLLNNQNNYKIRIQTSNYSRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPY3</accession>
    <name>A0A8S1JPY3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060518</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPY3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="144" mass="16633" checksum="9495BF0A78D8FEFD" modified="2022-10-12" version="1">MEIVEQKIKQDNIIVTNDLDGILNIYIFSICMWHIWTLCFFNSQKVLPTIIDQFIKSFIRFKNIICDLELLNIKGLKSSFSKTKKDLSNFELILQLMEQHEVVQFLRGGGGANIGDSRSVNKIPNNQIQMIDKILKSSTQQIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1D6</accession>
    <name>A0A8S1K1D6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0060270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1D6"/>
    <dbReference type="OMA" id="DEFQLDC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="122"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8044188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="492" mass="56969" checksum="28EFC37CFFE549AB" modified="2022-10-12" version="1">MNSGKFWNNQNIPQFDHNETQKVIISNFYKFGKRLGQKCLRQYILIRQYLLYSDVGNFKNIKGSVKLDSVYAQFQISNSDCQIYLKNNGFQIVLCTDDTNQYQLWAKLLSNCCILNTFNEDIILGKLIGNGSFSTGRNKEGHLFAIKAIPKKKSKLQTINKQYEEQLLSEISSLRELDHENILKLHRVYETTEKLYLVTEFIHGYELISKAASKLIYQGSEMKSFIHQMLLAIKDMHEHNIMHRDIKPQNIMLKNGQLSQPCLIDFGLAVSTKKKGFPFPSCGSPGYSAPEIIRFDETKKEYSGKCDIFSFGITLLVILYGFNPFKAQDQKQTIKKNANAYFEFPSSIYPQELEHLILQMTKKYPKDRITAEQALNHPFFQIKLSQTIQLPKAILTKQQYEMNKNYNNINVHASLEMDREFGLNYGIQLSSCSPQNNKNRTCTLSSNPNKLQQIDEFQLDCVEESIDITHLDKLKMGQRHFCKQKSQINVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1Y0</accession>
    <name>A0A8S1K1Y0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100231</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1Y0"/>
    <dbReference type="OMA" id="LNICAFY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="830" mass="98250" checksum="2E94F91132C3B43D" modified="2022-10-12" version="1">MYQQGEGYKDEIYLDDDEVFKQDEEPSIFDIVYQEVGCILLKYTNLNLITCPNTLHYITLNFNNDEPISFLFQPNYILVEQLAVNPALKEIADQLQIEDLINHNQNDHEYFSDYYQHLIKETFKSHINQAQEIFQKQVELLQEQRNMPQVNQELQSIFLDKQFQFPQKLDYKNSEIPENEIKYKLFESIQILQSNYLNNNPIENKSTKLECSDTAQHLSGYKTQSECFDITPQVQPQVISSLNKEKIKMIVNWLIQENIAIPEIILCAELKNRGNGMNLTFNPNQQMLNICAFYYHSFYDAQQNQTETKNIKEENTDEQKINQQIKVKKQNAKIIEYLLLIRMIDLVVQQQESLKSKIIQLIKYPQLKNQIKRLSRIQEVDAHFKIVKQFIEKLETILLIYDETKQREIKNEIEQKNQTQSNSLSEIDDESKTIKCQKKYYRLLKLILKLILIESIQQEIPIPQILTCLWFKKHKEKIVEGQIIIQNSVQILPSKLRLIPYLQQENRLKLKLEQVQLQSFLELPDTSTQELEDFENFLQQFKFDNKYCRDVVLIEKILIIIHNKKDSIKDQLTQVMQDQLKIEKAKEQFKDRLVLLPKLRTNKTQINDEPILENIIRIIENLQLYICQSKNDNLMINENQVNISKKQISTIQKQQMIESKKLDKEGLKVFILSGLITLIDNEVPLPDLITYLGIKNKSQKFSLQINKNYRLQEYLTTQMQLNKVEITDQVESKLLIKKIKIQDGENITIIPNKEQISEPQLIIRALKLITNCEMTKQLSEIWQRPSVLNLKNQVYKNSLKKTKNDTTIGQIKKQFNKIDNKYYIFNNNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4K2</accession>
    <name>A0A8S1K4K2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0170023</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4K2"/>
    <dbReference type="OMA" id="CITNDCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08215">
        <property type="entry name" value="STKc_Nek"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000535">
        <property type="entry name" value="Serine/threonine-protein kinase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="29"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8050390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="548" mass="64362" checksum="F0A69F0DB6DC9CD1" modified="2022-10-12" version="1">MEDDSRIINDDESCITNDCQVNETQIKQYKKVKLLGVGAYGKAYLVSSDDQDEVKYVMKVVPQSISANTEASILQNLRHDNIIQYVETFIDNKDRLCLIMEYANNGTLGQYLKSRTQPLPEAQIIDWFTQLCLALQCVHSQKIIHRDIKSENVFLHDEKIKLGDFGIARSVEQDLATTFIGTPYYISPEIIQNQPYSYKSDIWSLGVLLYEMCTFKYPFTADSLPALANKIMKAKIQPISAQQYSQNMKNLIQQLLQLDANKRPNIEQILQNALIQNRIKQLNIKQQQIIKIPVQPMQKKQQHQVVIKNDEYTQNKKISREEQQKFMREDIQKKKCQQKPQEVVIELFGQPKIKQDQIQQQQSQIDKKLPKRRDIPVEIYLPYISRSEFLDQQQQQQQQQQQQQQPQQSQSIEIDINNNNNELKLEKQFSENTQIQDIPNQKLEKQNSKKQDNIKCVPQDTQIAQKPIIKLSFERAEKIRMNLEQVFGTDQFLRLYQIFRGLREKFSFDEISQQYGPTYENLIPDINQQCMQEQLSNLFILLEFDLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K602</accession>
    <name>A0A8S1K602_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cystinosin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0170198</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cystine(out) + H(+)(out) = L-cystine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:66172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:35491"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:66173"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystinosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K602"/>
    <dbReference type="OMA" id="ASWEWID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015184">
        <property type="term" value="F:L-cystine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000016">
        <property type="entry name" value="Cystinosin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005282">
        <property type="entry name" value="LC_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131:SF5">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8050719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="31660" checksum="9BD08353A21D2154" modified="2022-10-12" version="1">MLDFLLSNQGEEEPKSQALNVISNIIGWTYFVCWSASFYGQLYENFKVKNVKGIAFEFLGLNLTGFLFLSAYSSAGYFKGGDNGWPFSGGITKQDLFFAYHAVAITVLTILQTAYYYKKGDNPGVSQWCIIVLIVLWSQTILYVLLTWIFGWDVIFKQEKLNVLYWMGYEKLFISFIKYVPQVYLNYKRKSTVGWSIFNILLDFMGGFLSFLQMLLDSLNGKSANLVDVNIVKFILSWIAMGFDGIFMFQHYILYNPKKRTRDALLDEYSIPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6J9</accession>
    <name>A0A8S1K6J9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0170014</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6J9"/>
    <dbReference type="OMA" id="HEKYLYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF155">
        <property type="entry name" value="DEUBIQUITINASE MYSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="3">
        <location>
            <begin position="36"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8050381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="154" mass="18274" checksum="22B136C8BAB73013" modified="2022-10-12" version="1">MSLNSYEQQLDLDDSCESPFSQSPKSKRIKKFELISPKKNVGHWTKEEHEKYLYFLEEHSDIKKNNKIFKSMSEVIGTRSPSQCRSHHQKFNPLSPLVQRKTQKVQKQTMSTQQDDLNLQIEEESLNRNMVKLMIFDEDDQVCQLPQFNLDDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAJ3</accession>
    <name>A0A8S1KAJ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0190043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAJ3"/>
    <dbReference type="OMA" id="HFMTISK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00177">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000044">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF56">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="START" evidence="1">
        <location>
            <begin position="25"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="209" mass="23912" checksum="717826C9DD5F3A2D" modified="2022-10-12" version="1">MQNNYDQLLEETKTKILAIVELKPENQWTLDTNKDGYAIYTRNNPDNGLKINRTETNAVVNPEEFINFVIDMTKKKEYDSNFQEGRLISKVDQNTFIFYARGKPPTFFVDARDFCILTRVYKLGEDHYMTISKSIEHPEAPAVKGVQRGEIVFQAWIVKKLPNGQSNIIIIGNMNPKGDIPKAIINQGAQLQAEGVKKAIEYLTKNNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KC69</accession>
    <name>A0A8S1KC69_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0200300</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KC69"/>
    <dbReference type="OMA" id="SINMAYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000104">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8053380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="263" mass="29316" checksum="6426C9B30BA06E84" modified="2022-10-12" version="1">MGEINADTAYVGGVKIAKQEFAEIVRENGDVFAQSDFDGIVGLAYPSMAAYNFNPLFDNIMQQKLLDRNVFSFYFSRQEGSRSSELTLGGWDTDHFQGELHFHNVVNKYYWLLDADNILVNGQDVGLCKHGCKVVADTGTSLLTGPSDDLYDLLDTLNIDENCKNAKELPKLTFVLDGINYDLDANDYVMKIDSQGNEIAYDTFASIDSFVEMGANCQCVGSFMPLDIPSPQGPAWILGDTFLSKYYSVYDRDNDRVGFARAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCP5</accession>
    <name>A0A8S1KCP5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="12">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T0130030</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="12">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="12"/>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCP5"/>
    <dbReference type="OMA" id="CIFTAHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000051">
        <property type="entry name" value="Helicase SWR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020838">
        <property type="entry name" value="DBINO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13892">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51413">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DBINO" evidence="17">
        <location>
            <begin position="217"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="428"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="890"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1067"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="286"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1078"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1109"/>
            <end position="1118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8048674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1124" mass="133841" checksum="D42C798F616C56A2" modified="2022-10-12" version="1">MSSGEEEDKQYEIDQLLQKITEIENTRQVNRHQRKVEEPQFNLVCFLKRPPVEDLSQEEARLCYLENKRQMVFSKKHIEEKQMKHFYSSNNSSKIQSKLLTFFSKVLRDQPNMVEKFLHEKYNHKLETVFEQKVHIGITSQQFEPSFQPTKLPLLGYNFEDAFKIQYADNRIGRVNKQKQRKTKNEYQSTTQNEYFNTIIQQELYEDPIEVGDQKKSWAIVVRNAVKIARFKAKFRQDFREFFKRIAYFAAKEARRRNQKCQKYQKDFMMRAKKLSKEAQAFWRKRDKELIEIKKRKEKLEQERKKKEEEEREQLLQQKRLEFLMKQSDIYAHFMAKKLGITLDNKIQQTNGNIEIDEAKAFETVQKVINDNRKQLQQFDGKEQENVQIQELKLDHNDQDRDFSLIAPPSIFHGDLKEYQLKGLRWLDNLYDQGINGILADEMGLGKTIQAIALLSHISSFKQVWGPFLVIAPSSTLHNWQQEIKKFCPSLKVLPYWGQAQQRKTIRKYFQQKNFGQKQSLFHVVVTSYNLVVSDNKIFNRVRWQYMILDEAQAIKNINSQRWQILLSFNARNRLLLTGTPIQNTMGELWALLHFIMPKFFDSFDQFQEWFSKDIEAHSQDQKTLNQHQLQRLHAILKPFMLRRLKKDVENEIGQKKEIQIVCEMTSRQAVLYKNVKSKLSIKEFFRMLDSKQKVDNLMNLVMQFRKICNHPELFERKPYKTPYIFQDKQNVEVYQQNPIVQVTKRNPIKFIIPKLVYDNLIEDMPCIFTAHYIYTSLKNGDSTFAFFVLKHLPLGLFQKNIFDLILLLCNFNEPKNLINYQFSKQLQFYISIAQSQIITFECRSSSFYQKLNQSLYNRQALSFIKESQACFILPNNPDSLIASSSKLLQLDKLLKELKQKQWRVLIFCQMTRMLDILEEYMLHKGYTYFRMDGQCQINDRRDMVNEFQQNDKIFAFLLSTRAGGLGITLTQADAVIFYDNDWNPTMDAQATDRAHRIGRTKDVYVYRLITKGTIEERIVKRAQQKQNVQSTVYSGGFQGDKFKPQEVFELLFDEQDMDETVANKFMAKGQKKKKKPVKQDQKDIKDQQKDQQKDQKEQQEEEEFIQVDLRELEMNEKDCEDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCT2</accession>
    <name>A0A8S1KCT2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH CENPB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0190172</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCT2"/>
    <dbReference type="OMA" id="DMYFTAS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03221">
        <property type="entry name" value="HTH_Tnp_Tc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH CENPB-type" evidence="2">
        <location>
            <begin position="65"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8052417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="244" mass="29097" checksum="4F8A68A2E9906C54" modified="2022-10-12" version="1">MVDLNINKKERKIHSYKVLGTNQKKEALEELLNDSSQENFVLLSKKFNTTTRNLRRWFNQGYMRKGGCGRKKINPQGVIRLEEWILEETRKQGRKISRNQIKEQAIKIFNIESFKASKAWMDKFIKEQNLKFKIKQILLENGLLSKCQIIKHKQIQNNLKEKKFVRSSTKKQQKRIKLEQLNTKYIMGNLDQIIVQDIQIGQKQNKIKLENNNKEDDMYFTASFEQEARQFENNYGEQLYLGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KD77</accession>
    <name>A0A8S1KD77_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0180261</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KD77"/>
    <dbReference type="OMA" id="KRHIKWN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8051645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="155" mass="18504" checksum="8DC50A4E2E1A6A87" modified="2022-10-12" version="1">MEDQVSLVRYFADVSKLVTICYKLSLEKQALLKQTKCHSEYQSQSQQQKRPMEIESPKSFLNKSECNIQLQQEIQNLNQDQIKQKQKNLSKTRSGKQFFKSQIMSNKNQIDKVSQDNNNTFNCQQCDKKYYHMKSLKRHIKWNHQIIKLTNQIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDJ2</accession>
    <name>A0A8S1KDJ2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDJ2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="69" mass="8259" checksum="532D51E6BEFABBF8" modified="2022-10-12" version="1">MPESAVLFILDTWVHLPPKILIQWRILFYQDPSLNEGELNQMKDADLKLQMLQSNKDDKHNYLYSIRIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KE11</accession>
    <name>A0A8S1KE11_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0200273</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KE11"/>
    <dbReference type="OMA" id="MMACCGH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="61"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="127"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="228"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="386"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8053328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="50780" checksum="9AB45F4602384C8B" modified="2022-10-12" version="1">MSDMQEIEKEKKFIEEYFKQISTSDIDTIKKYLLYVKQRKRGNENEKVPIRTFINVISEGKGKKAIHFGASRGDLDVFKFLVKKGADITLLDDDKNNPFIIAVQHNHIQIVKYLIEVHHVDVNYQRNTITALHLAAQQSAIPMIELLLQAGANINALSNYGTPVSFAVAYQQNQSALHLIKKGADLNIIQNQMPSLLHLIIDQNNEELFNKIFEEFNDKIDVNIKDPDGWSALHLCAEKGLLKFSQILIQFGADINYENQKQTPLDLAVQNEKWDCVQYYREFALRKEAQIPSPPKQLTDEQIKDVYNEINSEKVEANKLLQSEQYEQAIIKYMEIIEKSKQIIEHHRDEIVKIYTNLANAQLKLSKYKDILATCKQARNIKKDWVKIYYYEGQAYEGLNEFGEAAASYFEALKIQQEPGMKALFDQAIKRGKELYKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KFJ1</accession>
    <name>A0A8S1KFJ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KFJ1"/>
    <dbReference type="OMA" id="NEYEAQS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="450" mass="52467" checksum="0D8BA8B2BE1AEA7E" modified="2022-10-12" version="1">MVQVIKVSKSYINYLNENLLFYEQQIVNILIYVVVAELSIITALMFMIIKALQDVNQLIRKILILITKLSEDDVNQINKIYTEIKTILKDPKQVQWKKTNFVKCIFETNFIKNKQDLNFLQSSNASSNSQNNKQKFKKRYQNSSLTSRVYNLRLPQIWNYAFIFLSWLFLAGYLLGALGLSLSMVHSIKPAIALNLQLIEFKLKFDSLIVYGEILKTDYLVNLKLNQFYELSSIKFNQDGVLTEFFSLQEGFQDQLYDIYDQLSSSTSLLEEQKNTLLIPFQSSLCESDPTLVPACTIPLESTSSDYSLDYLSGGIVDMVHEFSKTLKTFWQITDFAITDEDQLEKFLSGQVYTTEFVHHFLFQGKIMEQIAYTMLNLNKTTIDSVIFKFQIYMYIMGLTLIIMYIFCFYKWILRLDNEMYLTKLALTLIPADFLNEQKTMSDLKNILNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KK55</accession>
    <name>A0A8S1KK55_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0200338</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KK55"/>
    <dbReference type="OMA" id="DMYRKPM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="11"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8053456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="480" mass="55369" checksum="50C70F5D907E7B65" modified="2022-10-12" version="1">MQPASIFNGKYIVQKKISSGSFGVVLLGHDKDKNIDVAIKIEKEENEDVRSLEREVQVLERLNGIEGVPQLYWHGEQDDYNIIVMQILGKDLAYYMKSKKKFSFKTAIQLGIQIVKVLERIHNKGVVHRDLKPENILFGIDDEFSKIYVIDFGISKIFRDKNGKIHPFRDNTSFIGTTRYASIAAHKGYELSRKDDIESLIYVLLYFMKGQLPWQNMQNVSDEERTVKVGEMKMSIDPKELCKDVPIEFAIILEYLKQLEYQSEPNYNFVYQQFEKAAGNLGIQLDYSFDWDQQQPMKVSQNTHSEIMQRSTTLNQQLQLPSTGEMKKSLDKQGSNLIRQMSNNQFLAPPLSNQLQPHTLKRQDSSQQPSIVSGSVMLSTYNSIRPIYQSSQLGVDVKLSVDTMPKELIQEQRKTNDPAQQKNVQNPYTETVGWKDSIFEENFDEIPLSKKYGILQQKGVDMYRKPMHSSASPNRQKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKE0</accession>
    <name>A0A8S1KKE0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0240062</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKE0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="209"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8055960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="350" mass="41499" checksum="0C20E51977BDCDDA" modified="2022-10-12" version="1">MLTNQVQMLKNQMQTLNDVKLQQSFRLSFGMRTSSNKDSENNPTIANQSTSRANLSTYRERFKTSINSKSKSPFNLETPKKNKLLEEQQRIIQQLISKIPDQQQLEGHKRTIEELEDELNKKNQLIIELQDKIQNEEVYLTLNQRILEINKNLDNVIQQNQNLIKENDTMKFELGQLRFLEEQYYATLQENKILQQSIINNDCQYEKILQQYELDCNNYRQQILKLQEICEAQQAQLQGQEEQRSFQYQLQQFDKIKKLIKDEVQCIGQLIAQINQQLQKQLSDIMPNSIKQLQKDLIKKKYVIEEKIKSLEQLQDQDCELGKIQNNQENDELKLILLMQSLTIEKMIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KM98</accession>
    <name>A0A8S1KM98_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0210305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KM98"/>
    <dbReference type="OMA" id="FEMHIHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="412"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="256"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8054116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="640" mass="75492" checksum="6A1C61307CA23EB7" modified="2022-10-12" version="1">MLQIPKKSITYEKILADFYQQFISQEIASQRPQIEFLHFLCSSFMMEKLYKIYQESLLKKEDEQLQARKELNEFIQYMVNQNAAYLNESIRDELLTLSKNRHDDQYLSGYSQFYRYCPITTTSIMFEKINYISEYLKHQNEIFQDFKNKLKFNQYSNSDYFSTNRYATLSGLFKFNKEMESIQINAKDDKWRDLKLIEENMKTKEKYFNTFIEDDWLRIIVQIEKCQKSHLDPLSQQDFSEIQQLIEQKQLPYQIANQMKQEVIKKLQQRRQQQQQQQDNSNEKQVKYSQLIKYKLSHDTKLIECLLNQQKNLLGIKLQDLPNVISYIQISKFCKIICCGCYDGNILIYDMNELKQNRQNRLLCNPSFEMHIHEGPVTAISILYDENYIISVSVDLTIKFSCLKSRQPLVVYKGHLQTIWHLAFAPMGYWFASASSDGTALLWATDQNQCIRIFGPVMNQVKLVEFVPSVEYVVTAEENRIFRIWSIDKAECMYISFLDGNITCMNVSFTGQFLCIGCDSGSVVLFYLPITRLLKEKIYLQRSDFHSANGRDYIRSVSISLDETTLMCCNSNRIGFFNISDLMKKTSEDVFKGLEQSIKSIEYEKNNWSRNAIPIELLELQDYIHLAQYVCNGFALYVCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQB1</accession>
    <name>A0A8S1KQB1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0230145</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQB1"/>
    <dbReference type="OMA" id="MRISFNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14497">
        <property type="entry name" value="PTP_PTEN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF60">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE TPTE2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="5">
        <location>
            <begin position="13"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="97"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="6">
        <location>
            <begin position="188"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8055372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="42321" checksum="7A29FFEF29ED385C" modified="2022-10-12" version="1">MDYIRTIVSGKKKRFISEGYNLDLTYVCDRIIAMAFPADGFESCYRNPIDKVVQFLENRHGNNYLIMNLSSRTYDYSKFKNQVKNYQWNNHHAPQLHLLFNMCKSMQEFFNQKQENVVVVHCLAGKGRTGTLICCYLLYCGMFNSVNDVLQYYEKSRFNDEGLGVNQPCQIRYIEYFNQLLQGEPIYPSLKYLSSIIIEGVPKLNIDDGSKISAKIYQNQQLIKDTQILKIIQENQTNCFIMSPNPIVIHGDILIEFYNSNLVKTKLIMRISFNTSFNTITFTKDQIDPFKIKNDQRIPEKFKIHVCLSEYCNNCDQTTSFHDKCPDCKQTLNQERQNWHQIKEAIKLKGVNILRNFPQNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSV3</accession>
    <name>A0A8S1KSV3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">V-type proton ATPase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0250182</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0290179</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSV3"/>
    <dbReference type="OMA" id="MTYGYMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016727">
        <property type="entry name" value="ATPase_V0-cplx_dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11028">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="45299" checksum="8BA15715BB81309C" modified="2022-10-12" version="1">MDLCVFGVDDGYAEAIIRGLRASFLTEAQYQQMKNCASIPELKSFLEETDYQNCLQADNPQIPTSILRQRLKKKLADEFEYIEAQSTGTLTKYLFHLRCRFMIDNVVNMIEGLKNKIDIEILLSNIDPLGWFPEIKNIKVLEGDDYSSLYRDVLIDTPIGVYFMKFLEESIENLHENRTLNDIQNLFREMKPEYIRTSLKKMWLEDFYLFCEQELMPTSQEVLLELLKFEADFKTIQVIYNSIGNRDLNTAAKIITTRKQLCPTIGNLYPDCEKMYLQAMTLDTLREAVKGCDNYRDLLKDAPDPLKREEFNVQTKTLDDIMYDDECRRYALAFDGQGSYGVFYSYLKLKEQEIRNIIWLAEMISRKLAKNHPGWKKIIIPFSHLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KU27</accession>
    <name>A0A8S1KU27_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0270165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KU27"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035738360" evidence="1">
        <location>
            <begin position="22"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="70" mass="8351" checksum="FB3AC41CCEB351AA" modified="2022-10-12" version="1">MRLVIIIMLGKLLFFFEKVFVRNFHFEQAECYIQTIIQLNLMGLVNRNIKNLEKNVNNVQITLNEPLQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L054</accession>
    <name>A0A8S1L054_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0300005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L054"/>
    <dbReference type="OMA" id="HEDHDSA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="200" mass="23588" checksum="524091D12AA58A78" modified="2022-10-12" version="1">MGGDHHEDHDSALSHEIKAEQIKRLQPFYQQRLPYILRPFASLIYHDVPTINTDNYEHKSIENYSESQIINRTVFALQKLQLLNPNFFTPQENRILSPLPLGIVSYTLQFTCSAMNAIYGLYLYKTWSFNAKSFGLWAVFLATQQVCLQYPNFLCETLIQWPRRRRLAKQYLEIYGPNYFHQIIDPRFSVHKLETLKNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L080</accession>
    <name>A0A8S1L080_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0300275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L080"/>
    <dbReference type="OMA" id="MQANPIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF89">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="2">
        <location>
            <begin position="42"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8060717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="320" mass="38264" checksum="1CC833CD08CB9947" modified="2022-10-12" version="1">MQANPICVQPEAQYLDNYDNFEEVTTTFKCLQKINQPLEPEATFFLIRAPTKDNVHRAIKYGIWTSSSRNNKKLNDAPRPVYLLFNVTQTSHFIGLAKIITEFRDKMHFKYWAEENKWFGSFQIEWVFLKDLPYNELSKIQQSCGKCIHELIDCTQIENAESIYSSFQQQPQKSCMLKSFKELDISEKRKRNERDTNPNFDIQFQEHISVFEQMPFSFSVSSYQRRKQQQFQNQQFQQFQYYYQSPWYGVAQPNFWNAQSQTYPQQEQLQSQKVSNNTAGFYQTQNYNRPQTQNIQQTKHLQKKQLIYNKNSNQESPKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2X7</accession>
    <name>A0A8S1L2X7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2X7"/>
    <dbReference type="OMA" id="DKQKFTY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="157" mass="18597" checksum="D49BA79D8571F9FF" modified="2022-10-12" version="1">MIKRTFFGLQLRDKQKFTYKEENGLLCYTFTQIVLVGKGEAKIYGLSNNKKILIASLNGKQNQCKTKFIMDENNFTHLICIGDEQISVNVLGYQLNKQVQEQQENNQQENLVFKDEFRALQPVEERELDSDEFETLSKKEQKEYKKIQKQKKKYEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L331</accession>
    <name>A0A8S1L331_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0300234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L331"/>
    <dbReference type="OMA" id="KASSQKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="195" mass="22928" checksum="E1A770AFDE1A321B" modified="2022-10-12" version="1">MQSLIQAFQQKISLSASFVKQSTPQILFGISLLSCLYLLNQQQKEEKQVEPEKTEKKLKKKASSQKNLDDNKPQYMDVISDLKKSQKKLNKKINKQFDDSIDNTEENIPRSPDYSVVSEIDQKYFQELIQNQKKTKRAYQTDCESTPIISLRGFRRNQQLIGEEDKKGEDRLFYILKQRVIEQDEKENEIDEDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHL3</accession>
    <name>A0A8S1LHL3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0380038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHL3"/>
    <dbReference type="OMA" id="QGEMTWS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="139"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8065715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="164" mass="18654" checksum="FDD7BC1D573C58E4" modified="2022-10-12" version="1">MGCCINTPYVYPDGGVYDGELKNGLPHGKGKIQWKNGDYFEGNFEAGKKTGKGIFKWNDSSYYDGEFLNENFHGYGEYQWYGGRLYKGQWVEGKMEGQGILIDDGKQYQGQFKNDKKEGQGEMTWSNGQKYIGEWKNGKQHGKGKLIDPNGHVTEGQWIKGKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIS9</accession>
    <name>A0A8S1LIS9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0410064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIS9"/>
    <dbReference type="OMA" id="NCQVDEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="216" mass="25826" checksum="91CBF9F16AF14752" modified="2022-10-12" version="1">MSLRRFRSGGMHANSEEDYLNKIVKIEKQQKQQTYCQEDEYFDDFIVNSQFTFDYIQQPQLPEITQKSHFILIMIDDLQLSPRRLQNMIEYTDNCDIMTQSTNDDSSTWSPQIMNYDCIVHSYKSDYVNHLFNIWIINTPNINSTFQNCYFQLFEAFICVNNVKSIEFNDIFEKIKEINSNVQQFHMKEYDEASPERKQISNIQQILKSLIDSRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJC5</accession>
    <name>A0A8S1LJC5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370259</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJC5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="74" mass="9111" checksum="7F8B1AACE4904448" modified="2022-10-12" version="1">MPRKKFEFVIQNQGRGKMHQMKQEFYFQSKIRILQDTKKLFLMNNQIAYVSQWNRHLEVTLLNKLKFNSQTYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJJ6</accession>
    <name>A0A8S1LJJ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0390010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJJ6"/>
    <dbReference type="OMA" id="SLHPQIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="592" mass="69166" checksum="056755D88BA734F4" modified="2022-10-12" version="1">MNQQNIIEFFPNLLEQFQSILLNLYRNETLQKYHSIIQQIKTKFPLPEQMPQFIYPNVEQLSEFFLQSYQPIILDLATLYQKPNVKIKLYKNAVYFGEIYQEMRQGQGVLIKENNQIYEGYFANDKKDGIGFEILKGDTFYHGHYVQGFPHGEGVYKSISHSYIGQWQHGKKSGIGWCVGNKNDYFLGSWENGKFYGFGLHIHDTIYFGEVINDLKYGYGEEYFPEGDIYKGQYKNGLPNGKGKYIWKNGNSYQGDFQNGLRWGEGFWEQKTEKGSQFYKGQYVNDKKNGHGHFHYSNGTEYIGEFNDDQRHGFGEIIWPEKATYKGYWKQGLMEGEGIYSYENNKLQGIWKQNQLISQEKVRVSLNQFPLLNQLKDIVEDEEIQSQIAEEPDHEKIGDIFKKITLQSKTSLCISDTSKLPQLTSIQKQSDLFIYGQASSYPTQRTNQTSSIGIQTEINSTKKKLITELPYIQTKRKHVLSSYRMDSKTKITKKNLSESVTNTNSPNTRESEFKNNIIHQSQEKKSRKVKQRLSLLTKVEEKVLKIRLEKQKLSPYKFEKLWSKKVVNQARSLIYPPVWVPPSLHPQICRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJT7</accession>
    <name>A0A8S1LJT7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8064986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJT7"/>
    <dbReference type="OMA" id="NQCNHVE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="36476" checksum="DFF1AA25B9C76DA4" modified="2022-10-12" version="1">MGNQCNHVEINDNELKSVQIEDDSDSFLVYKFDPKLHFPIISDLRKLQSLDSLNSTKSQFHDTVKKVPFNVKLHRKRTLNYESIHDSQAQSPSNLVSEFFEYPNGVYQGTLIDGKREGYGQFFYHDGSYFKGSWKNDMANGLGKIKFQEGELYEGEFKNDQQHGFGKYISNQLKYEGQWKENLPSGQGIEIYNKYYIYEGEFQNGLKHGKGKILWYKDDSRYEGEFDENQISGQGIYYFGDGEIYEGEFKDGKMHGYGKLVWPNGKIKQGFGKFTSSDKEYTGEWYDGVYHGYGKLETKDKVIEGYWENGVFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LLR6</accession>
    <name>A0A8S1LLR6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0400250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LLR6"/>
    <dbReference type="OMA" id="GQDKTKP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="228" mass="26564" checksum="341AB25B7DEAB85B" modified="2022-10-12" version="1">MLNFQQPSLQPQNTTQNNLQFIYNEVLQMQQINETLKQHQNTTSAELEALKGKLEQNKTHLTQAIFQKTGQDKTKPNQVQNVGEFQGYVGQLEQSMGGQLKQIRDKNQLIIQELIKAHKLLEKYAISKRKLNKNSAKELGLIQDIEELRDRASIIFNRLEEHENKVQFSEEYTDLSKNPRIIDFNEQSIELIKELKKDYIASKITLDKSQHNIKSMLENLNDTNKQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LMS5</accession>
    <name>A0A8S1LMS5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0410085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LMS5"/>
    <dbReference type="OMA" id="HDIVHQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="430" mass="51111" checksum="1FE309EEF86E0985" modified="2022-10-12" version="1">MQENRKQMLKSQDSDRSIKNASDNITKIANKKIQQLAFLTQFPQQRKYTVVEKVNKLIDKTQSKLKHQQIPKDVQFYQRNIKKFENLSSFINSKGLLSANFSMPKLRVKQKVDDQEQEYVDYLLAKNYKLQQERQRRKSKQVKENYEECWELENSDYDFESPINVGRELKLAHQDQIKGEMEAKAKLWELQQIRKYQRKATKIDLKGSQRSSQYNNQEEDEIIIDQGQLNAQHYKEQSDKVIKMIWNAAKKIYNKKEDQIKKEIIMHQIAKNYRQKQRENAKEEINKILLTGQKQHLIFATTVRHLPENGDSKQNTEHFFRKKGQTSRKTQAPYSLSPPSQNEEMKSNRKISQINSRLHDIVHQIDNIPVGDQAHTIKKMQSEQILYQTLRETLQPDQIKDTLKGLFLPTDHLHKRPLQFNKKHFQNQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LMU0</accession>
    <name>A0A8S1LMU0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">STOP protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0440076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LMU0"/>
    <dbReference type="OMA" id="RSNYNDW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8069538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="46068" checksum="581602E33D61F256" modified="2022-10-12" version="1">MNQESLTNFLKQKPQSLLQQNNQIGSYRPKSQQKYCNQQQTLLRPPSSQIQSINGAVKLKQFIHKKNNLMLNRQSQTQLKYQQNEDHNCYEITPQLLSEISMDDPIWKRILPENLKLDEKQLIQLLDENTDYFYHNLKLCACFKCTCGRCKCDATTQVKLKLNGIFLTNYDKDFVINHKSKKSLTNPLNQKTYDTKFMDQKSINLKSLYQQDFQQQPLELQPNQKKHSSQQIGSVSNLTSYRSNYNDWGTDYHKYIPYPHISTSPDIKFIGSSQYKESYISPNRWNINNAHYFKSCLTPIPTGQFEGQTTHKTSFIPYSTSHKSQIDRQNVYEKIPTFEGQFISTEMNDYIKKDDQNCPAKQFQKIFRKQMADKLLKKQQQFLKNQIQQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LN82</accession>
    <name>A0A8S1LN82_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S acidic ribosomal protein P0</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0440032</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Ribosomal protein P0 is the functional equivalent of E.coli protein L10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LN82"/>
    <dbReference type="OMA" id="DMNPFKL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030670">
        <property type="entry name" value="uL10_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039087">
        <property type="entry name" value="L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="6">
        <location>
            <begin position="129"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="288"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR039087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8069450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="323" mass="35392" checksum="0FB1D64718F098DA" modified="2022-10-12" version="1">MGKKETKDKKPAQFKKIYELLSKYQQVIIVGLANVGSKQVQDIRRILAKRNALLVIGKNTLFKKVLATRVQDLPETHEYYDELKKFGAAIRELDVLKNQVAGKIGFIFTDTPVFDLKSVIEENKVETPARVGAIAPIDVVIPPGPTGMDPASIQFFHALQIPTKIEKGQIQITKDFVVLKTGQKVGQSQAVLLQKLGKKPFLYGMEVLACYDNGSILNKQQVSVNLNDILAKFQKNVTNISAISLANGWVNESSAPYVLANAFKDLAAIGLQSGFIFDQIKQSNAPATTSAPVAAKVEDKPVQQAPAKVEEPEEDVDMGGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LWY7</accession>
    <name>A0A8S1LWY7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0450266</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LWY7"/>
    <dbReference type="OMA" id="IISPRYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46726">
        <property type="entry name" value="TWO PORE CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46726:SF1">
        <property type="entry name" value="TWO-PORE CALCIUM CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="481"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="515"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="593"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="656"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="682"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="341"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="378"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8070465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1223" mass="143649" checksum="F9378E957E348E77" modified="2022-10-12" version="1">MKQDDIQSPQLCDNFPPRLPNPRRSAIFKQQNAISLTKLDTRLQEKNDMLVAQIQKIVKHMPSDDKQLKAERHIYDAYYLRTIRNDIDLADKVILKYLWIIENSRIWSGYLLTLTIFYQIITFFEKPYPESDFYEAPEIGNIELFILIFLTIDFVITLILLATKKNDGGFQFNTKRMIKMLFYLVCVTDFINWKIDQNQIRFSRLIRPALMIFYSKDLRRNLKGIVKASKDLLLLFLLYVIIISTFAFIGINLIGQLDTVDLETQDYGDFFKLFSMLFMAATLDFYPDIMIPPIFQGTFYALYFVIYIILFLFLFQPIPLAVVYEGFRKHRMQIAIQDIIKQKSAMMASFISLDSNDAGFLTENQFKKFLKTFYHDQLSDDEVKVLFSEIDKDFNDKIQFDEFNQLLYVLQNSKKISLPRSKPLKCWESLRNFLNKHGLKKFIESNFFGLIMFLVTIINCGLIISAFFIEDLSVLAIFDTVDTVFLGIFIFECLMKIIALGIYDFFVDGWNVFDISLIFLQILFDYILFSFVTGNIVQSIKANRILRIAKIQKVFRLFRAFRSIKLVGYLLRGLEIFAHVKNLLYKIIICVPLILRLILPVQIVFFTYSCIGIYIYGGIQTDDQNPFSNNSCDPNEFRFLWGQCKYADFNSMGGSYLMMLQVFTASSWGQLVFELSFDTKNLVIPMIFIGSFVFLSIFLLALIGGLVWEVFTVVSKTLFEQEMEQFKPEERVAFQLNFQDEALLGSSLGTEYDIRNGTQLSNAIQLQKKTAILNDDNPDILEFRPRNVGLRVHQDDKLDIPVELDFQIETQIYSKVLRVGINPNQQSRKLKPQGDLAQIVEDELLEIQKLYCDYFIDYQEFLEIKFIKESHNIYNVTTEYVVHIRNEIKKDEMFKRKHVNISNHDLLIQNAVLRDTSEMYIKQQEDQYFKTEYGQKFELIKELKFQSKFKVESKILFSLMGILKFPKPNIKYFFQILYLIENYFTYQLLPDCSFFKLLHQMDGKWYMISIEDKQIVFTKIGAGPWTHDNLLFDQGQMRICENLKFMKEIKNTEVKSHIREFHTSMNKMAKQFELDITKIDVRSTIVLYQIKNERYAPPDSATPRMNLQKPIISPRYMTMSVKNVQVSQVNVEVNSFNEDDNPEEIFQMGANVDECHRHVSQTMIFVLSKIQAQESRIVYQNQVMLAQFVLDLAGVIKNYSDNFFQQLEELYSLRSKQRGVKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYA8</accession>
    <name>A0A8S1LYA8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0510226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYA8"/>
    <dbReference type="OMA" id="RMKWFEK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8073758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="44580" checksum="A974D82CE60B1642" modified="2022-10-12" version="1">MGANCQSCCANKDNDNSEIRLPKEKTKPKPDNQENDQKKEEQKVQVKEHERFTIDQEQQKQKKEQKKEQKRESAKEAPPKNADENSKKSQELDKQLNISANHSVSMNVASNQEGNDLIKSTMNSTERKKLPPIQLESGAVYEGEWKNGMRDGYGKQKWPDGSIYEGEWVEDKSSGRGKLTHADGDVYDGEWKNDKANGKGTYVHVNGAKYEGEWENDKQHGRGVENWPDGAKYEGQYYEGKKHGKGILNFADGSRYDGEFLQNDIHGEGTYIWPDKRVYKGQWKKNKMHGKGQIIWQDGRKYTGEYEEDKKHGKGVFEWADGRKYIGTWIQGRQHGIGIYYLQNKEVKVGEWNEGKRMKWFEKNEIDQLIEEQKIKREDLHQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZ18</accession>
    <name>A0A8S1LZ18_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cilia- and flagella-associated protein 263</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0500288</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CFAP263 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZ18"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051885">
        <property type="entry name" value="CC_CF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025254">
        <property type="entry name" value="CCDC113/CCDC96_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15654:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15654">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 113-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13870">
        <property type="entry name" value="CCDC113_CCDC96_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="3 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC113/CCDC96 coiled-coil" evidence="9">
        <location>
            <begin position="167"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="165"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8073260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="358" mass="42313" checksum="180B672D0A66C658" modified="2022-10-12" version="1">MIPEQQQGITQEEKELILVEELSQKLEGLYKDMEQIRRENLLFESYIMRNSKDLQREDEAEDKRQKTKKKNQAVDKKTMQLTNEEKYEIAQFESDTLKKNIEEGRIRSDQILETLRAILEETDKAITEIRKDAFDFQREILFAGENTRTGKIEAERIDKYFKEKLVQKDAQIEKYKQKKANIEQQITKTNNQIQKKEEMGDDLKFIDFHQLQIENKKYVKQIDEKNKKLLGLKIATGRISSVLIDEKNQLQTEIDQCNKLILEINDKNKKTAKTQEQTKKVKVQITSLTEKKKKFAIQLEQVNNATNEDIPKSMTYVLQKQKEEELVYKIRNTNRKIEIAELAYQKACKTLGINTKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M1T6</accession>
    <name>A0A8S1M1T6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0490026</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M1T6"/>
    <dbReference type="OMA" id="TNGMTYN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000032">
        <property type="entry name" value="Glycine-rich RNA-binding protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000950">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051186">
        <property type="entry name" value="RRM_HNRPC/RALY_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968:SF26">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="93"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="103"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8072245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="192" mass="22379" checksum="28DDBD041688CAEF" modified="2022-10-12" version="1">MGEIVPNQLFVAGYSRNKVTDERDVKEIFRKYSSVKEVAYKGSYSFVTFNNETEAQEALKETNGMSYKGQKLKVDIVDKGKSRKTGPSDSDLCFKCNKGGHWARNCPNSRSPRRSRRYSNSRSRHHRRRSDSRSYSSYSSSRSRRKNRYYRNKHRDSRSPKRQDKPKKRSISRKRSPSNSQSSKRANSDSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3N0</accession>
    <name>A0A8S1M3N0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3N0"/>
    <dbReference type="OMA" id="SNDCQEG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="110"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="228" mass="26976" checksum="003740CA7D021C75" modified="2022-10-12" version="1">MFSQAFRRGQQPLQQQQQPMNISFHNQLQAQQTKSFSQTQYEKENISQFLGSTKRQNLNESFYQEQQVPQISEVYRILEQLQQMMIVKFNDFQVVINDQEKAIRPLIEQNTQLNNQLNNVLQQSEKILSQLTTNLEKSEQINKRNQKKKSNDCQEGDLGAVQQLLKTLDDRLNNLSNEVELIAETNNRFQVHVMEIVNIDKLEHRENMQRVLQEMDKAKRSISKKIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M419</accession>
    <name>A0A8S1M419_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0510001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M419"/>
    <dbReference type="OMA" id="INDPCFL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="260"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8073372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="307" mass="36369" checksum="683A5AF5B55BCD67" modified="2022-10-12" version="1">MQNINSIKNYYILRLIYSLGGMALIYQVIKYQIKNFKYLLRNWKFSNKPQIFNEIIQQNQAETGTLQILNQQIIYGRTKQQNFAALPLEIQQKFQFLALKKQNAQINDPCFLIIDSQNYKVQVTQCSNIRFSSKLYQKVQIRDQRTENQLKKFGYNKIFFANLTEYRIKFGTPIIVIGDLIFNKNTKSVRCFKQHFIGDLETIKKYLQIQTIKQSCLLLFTLFIFSYFAYDFYRIHKKKNYKCSSLSKIFISKGNDSLQCQECKKQLIDTFFSPCGHFNLCNDCAKPYQNCPNCGEYVEETVRTFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MES9</accession>
    <name>A0A8S1MES9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-related protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0590119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MES9"/>
    <dbReference type="OMA" id="PMWDNYP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8078009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="596" mass="69302" checksum="FD8027041B2D84AA" modified="2022-10-12" version="1">MQEKPNQQQQDNVQNTIKSLQDKIQNIENLIERGSDCIIIQYGSHSIKMNKASEMLPKKFRNLIGYKVQSQPKQSQSTDLTVIDESLLLLEQKLIQKCALKAQPKSMRGKPRAKIEVDLRNIRGFVGKKEQKQPQCILAQMIEDKSVVFEDDIFTGEYLVRQPIKHGFFNLTDNYSIQDVIEDLYKLTLYGLSCKLEVKNVSDYYCILVIPDIFQRIQVKMLVDMLIRQLGFKGIYLHLESVLSSFGANLQQCCVVDIGYEKINITCVDDGVILPGTYVRKNFGSKDIDLVLMRQVTKRNACDQTVQLQSNNYGDLLQMEKLKEKACQLTQKEDKLNYNYELHCLRNQKEKRFFVQHNDGLYISANTLFESEPFNEIRKQDMHECFDFTGRLFEMQYDPEDNFEELSGGIQLCWYWGSKEKEQQYFIEGNQMLNPNYMIPLDHMIAYSIAQVQDPEIRQKLANNILFTGGGAHLIDLVDEVEALLIEKFQVMELNEIERVEVKTIIRDVRPINMAWVGATVLPKTESVSELWITSARWLGNLEPQKDELEDMINQFDGDVQQLEKKLIAFAKKDKEKDRSCEYGLKHLKEKIPFIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MGG6</accession>
    <name>A0A8S1MGG6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0610172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MGG6"/>
    <dbReference type="OMA" id="FFQQCMA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="123" mass="14190" checksum="660F47173EFE968C" modified="2022-10-12" version="1">MINSQSDDGEQPVSSVQAIEKPQKFSLFDLIVGLSIFSVLFFQQCMAEILFPIGFIFLMNGILYKCNRRIDQFMIGLFYVVQLFAVWALTYHDFEECRIGYVLVVIYVVVIFCFITSSMIILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MIF0</accession>
    <name>A0A8S1MIF0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0600117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MIF0"/>
    <dbReference type="OMA" id="KERYFKW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8078552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="322" mass="38127" checksum="09E5D1890D417107" modified="2022-10-12" version="1">MQTMTHLQLSGFSFFSSKNTRDNQHLIVKNKTNNQPKKRKLLFLKKKKQNSNSLPHSYRIPQLDDVQYQTTLSLTERIKKRYFQPLKNAVAKITEIRQQQLPRRKSTYGILFGQLTQKEMQNFPTAIEQHKQINKQELALTSFAIMLERKQRKQTTRKLSKKLSKQESLVEYDNMERPPSCKTPPKIILTSKPKSKSKIQLEKERYFKWLDLKMFHFQSNKLQKNNMPQQNQKVKQHFQTQSCASLRNLQICSFTSSPLLKARAKQSTEVNLVLTKQKSKSTLKTQRPYITHNNQLQKKIQQTFEIKNTSWLKQNSLEQKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKM5</accession>
    <name>A0A8S1MKM5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0640240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKM5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="165" mass="19364" checksum="7C2B7BF5636DE665" modified="2022-10-12" version="1">MEEIQEYIQLNLTFRIIPLDKENNHIKALDGSKCTLKSRGIKNGREKEFLNNFDTITQKLGFLNNEQSKKGYFWEIFKILEKGLIIIFLTFYGDLIIIKRAYVFTIVFIYQVLTRKQYSQHIPQLNLIYELGTLICGASKVISIKIYQADQSYNFSFLYLSHSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPH3</accession>
    <name>A0A8S1MPH3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0660010</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008318">
        <property type="term" value="F:protein prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="185"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="219"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="287"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="321"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="355"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="389"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="423"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="457"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="491"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="525"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8081469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="583" mass="67681" checksum="F11CF307446399DC" modified="2022-10-12" version="1">MEIFKCKYIAHENEEIIGFCLNQSCQNATQFCYQCLNTTHSEHFNDCIRFSKIIQFIKEFVLNYNKRKIQFQEISQQIQNCSECIFKKMDLDITILEKMTQQLIDQEYLTFKSQIPIIKQFYSKEKENYICILLQLNIILEAIKKMIPQEIQVDDKNFSEYQDVITMVEIKQGSDDVKQQNIQEAYRILNKGLALHNLQKYQEAIECYDQAILINPTYYTAWNNKGFALHHLQKYQEAIECYDKAILINPKSDAGWNNKGFSLNNLKKYVKAIECCDNAIQINSQNNSAWSNKGFALDKLKKYQEAIECYDKVIQINPKCDVALSDKGLALYNLQKYQEAIEQCEKAIQINPQNNSAWNHKGLALDKLQKYQEAIECYDQAIQINSKYDIAWTNKGLALNTLKKYKEAFGCYDKAIALNPKNDTTWINAGQALYNLQKYQEAIGCYDKAILIDPINYPALNNKGFALDKLQKYQEAIECYDKAISINPKYDVAWNNKSLALINLQKYQEAIECCDQAILINPQNNSAWNHKGLALGKLQQYQLAFECFDKVILINXXXXXXXXXXXXXXXXXXMKMKKQLDFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQ86</accession>
    <name>A0A8S1MQ86_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 12">Ornithine aminotransferase</fullName>
            <ecNumber evidence="4 12">2.6.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0640068</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-ornithine + 2-oxoglutarate = L-glutamate 5-semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:25160"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:25161"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>a 2-oxocarboxylate + L-ornithine = L-glutamate 5-semialdehyde + an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:13877"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="4 12"/>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQ86"/>
    <dbReference type="OMA" id="RSAWDLC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019544">
        <property type="term" value="P:L-arginine catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010121">
        <property type="term" value="P:L-arginine catabolic process to proline via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000011">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000152">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010164">
        <property type="entry name" value="Orn_aminotrans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01885">
        <property type="entry name" value="Orn_aminotrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF18">
        <property type="entry name" value="ORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8080641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="420" mass="47433" checksum="388E01B71B28F0CD" modified="2022-10-12" version="1">MKNLMFKSIARFSQVKNSQYFVQLENQYGCHNYHPLPVVISRGKGIYVWDQEGKKYMDFLAAYSAVNQGHCHEKIYQELVKQASQLTLTSRAFYNNKLGEAEKYITQLFKYDKVLFMNSGVEAGESAIKFARRWAYNVKKVPENEARVLFANGNFWGRTIAACGSSDDPERYNKFGPFGGLNFDLVDYNNVNAIETKFKANPNYAAFFFEAIQGENGVIIPDQNYLKQVRSLCDKYNVLMIVDEIQTGLGRTGKMLAVEHDNVRPDMVLLGKALSGGFYPISAVLADDQIMLQIKPGEHGSTYGGNPLAASLCIKALEVLQEEKMIENANSLGKVMEKRLVELKKYNNVTQIRSRGLMGAIQFQDGKGDVAWEFCLKLAKEGLLCKPTHKTIMRLTPPLIINEAELNSAFDKIEYVLKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSR8</accession>
    <name>A0A8S1MSR8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0690182</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSR8"/>
    <dbReference type="OMA" id="TYTCMIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF0">
        <property type="entry name" value="UNC93-LIKE PROTEIN MFSD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8083240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="342" mass="39572" checksum="302A33D114D4CC61" modified="2022-10-12" version="1">MFEKRDNLCSLTYTCMITVCYLGVIGLCRTVLFSNILKIDDKVINSVSLGIFSLVFLLGPFSLPFFQKMIMKFTYKAVFFITSVANIFSMTLYIVVIEQQPKDVYLIATILIFEAISAPFMAIFYCTFNYYIRSLSNKNNVGIYFGVAYSLFCMQNLIGDIYVIFEEKIYEYNQYFYYPMLGVSLIITILYWFIKEPDSLSKSVTKQSIDLQQKLNDHLYGSYMNDDNTKINTNNNEYINQFKLIWKIPKLYPQFVYLIPTIISIGMFTAFSVVYSQDMIEPNYTNVNYLKQDIVANLSIHGIGQFLGGILIGLLSYSYGYLNLLMVLQVFGAVTYIFSDCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTG0</accession>
    <name>A0A8S1MTG0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0690064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTG0"/>
    <dbReference type="OMA" id="DNFRTYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="844" mass="99769" checksum="88D8A818AC85899C" modified="2022-10-12" version="1">MDKKTKYKLLGLTALVGLTSFFYIQRERNRPISQKMAQKFKELQRKLDSYFQGKSAEEMIKLVTNNDEFVEMIQKEAGDFFDLKKIDEEISKIDFIRQLSTINQQINQLLEIRLVVQYIQKNSLRQVQEQIIEKLASQRVTIIQYNTQFGESIINFLQYVQCSFLQLINELEQLLQDLRSPQRIVAVNYYFMCILDYFNNIFAQYPNLKNLVAIIMLEKIGSNQFSFDFYHDCTDNYYVNTVKTCQRLKSCDCCIFDLLCRYISIFQSPVQQKFLEFIKFNQLFQTQEHLLQLGTILFKNTFYLGGQLSEDSLHFIDTFSPIHSLLIEATSNKSVQEGLLQSEQFGIVIQYLEKVISNHENPFLNRLLASLIDVYSIIYPLFYNESALKQIVKDEEKIKQILSKFFGALLNLESRDTYDSNKQNEISILDWFALNMGTIFTQQLLGNISVEFIQQLLKVKEIEREILFTYIYCLRVSQNKRIQDRQQKKKNVDINSFITKLISLIVISLLKNDFSNKNMDLIINNLGFVDDDERSNIFLNILKIQFEIPSMNLNYLKYVSAKCRQLSQETYHSFSGFYTNLLNPTTLSFDQYMISMQLGMIYGKVDSFFAQIGQGFLRDLQQFDQYKDFLQRDLILIIDLMENPYYFANVLIHNRKCPQDLYKQLLTKVAISIWQLDPKITFSQLQQQMSLFIMVEILPQYIKDLFIVNQDGTIKLKEQQQQLWPFLCLQRDCSSVEIYLKQVEDEVLFNELLQESDSFEFLTRIQETMFNSSNFIQIIYQLLLCVHRLEIKTWIGKILFYANKFKHINKECDTLLKSNNCQSFILEMPDNFRTYMLFGKNVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTZ7</accession>
    <name>A0A8S1MTZ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0630165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTZ7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="994" mass="118530" checksum="7C8273BEB48F729C" modified="2022-10-12" version="1">MELFIELTRQQLSDDCYIEFPKVCNEIGTKWLIGYNHIITIDEDYEKYKNGIRKEQAQDLLIQDINKYKIVLQYEYNEKYGEKTFQQLNLGCQYLLLSIFINSGRILQFSKLIYQCQQNNFYEALKSNGRNHINNENQNIKLNIKCQEEQQFFIEHFDEQGIPKQEGIWTIEIPQTNEEDLYYAQQILDDRFYTLGLCSIFAINTELEFCNYKSVLWLEFLKDQEGKKYNFVSYNQEQQKWFPVNVMDQEYQIIGYSHICNPTEVLAYQDGLTDGQVNYLFLADYDQNCLKVRRFLKQEYQNKDFDNLTSQVQLLLIDLSFRNWPLIYYKKSIDLALENKIQEAINQSYIYYIPCESVNSNYLIRKDEIYTFLNQISSTYQLKTENNNMFLENPNLNNIVGVKITEDNFSFILKEEAETKIQIEDLITFLKVYYDPIQQVDDITFSLEPEDNDEEMMRPFQNKIYSPEYLDRTNVGLVLYEADFLLKQMSLGIEVLEREPLITKPFVYGNLDIQPLYKLDPEYYARLFLKIGKLKLNILEDKHNNVHKLEIEKIDIAVEARILNQQLNEEVIQDENNGAQRFASQFTQLYDQIGQKYPIFIRLRQVLMANYLAKYMYEKGIPINYEFIENIFYQKLIPQEQLYRKSPTLKLYQEDEYHIIDLRGGIDSNSSQIDNIMKNALNHLDENQNLQVVETNSEIIDAKFQRCDSVSSISTQASFEVINELKKKEKCMNCKKDLEQCEIDQNKICKTCNNQKCSKCYKISNENLFKIQNEICCKECFKCYLCNKQNPNKIYDQKYYHQNCFEQAKKQFELKKQMISYIKKTFGTKQKQADNNEKQSYFQIEQYPFINIRDYTGYIIGYNHPIIKSQAQINLKQYKLPFKKAEELLIKDLEEFENQLNQFYKVQNYTRDNNFKMLMLFILFIFKNFDQVLNLANKSNYKDLFVEIPKLQIKNTKGVHVLRGKIEKALDFFKLHFDQNGNAIQNQEQKIKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MUV6</accession>
    <name>A0A8S1MUV6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0650046</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MUV6"/>
    <dbReference type="OMA" id="EFQKSIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000592">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="4">
        <location>
            <begin position="3"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="105"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8081096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="219" mass="26228" checksum="7063B49E9E8B556D" modified="2022-10-12" version="1">MGKPKILIKNLYEILQSEKFDCIRWTDNGRAFIIVDPEEFQKSIMPEYFYSTQIKSLYRQLNSYGFKMRSLEINQKMFSHKWFIQGNISSLYKVERQRTKSIAHLDKEKDSIVIMREEMKQLKEELETLQSQQNSILKQIRIHSIIQTYICKNIKKICDEQKKFNLEREQETRLVIYFTSMFEIFKGFQFEIASNIILQLKDIGGPQTPSFSPMIFPYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MV21</accession>
    <name>A0A8S1MV21_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0640102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MV21"/>
    <dbReference type="OMA" id="ELMKMAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="271" mass="31641" checksum="9E3605658B7ED9A8" modified="2022-10-12" version="1">MQEIDLLRLHRETLKYLEIESIFKRLTQTFINKIYNLIRDIFAEEHKFTIQELMKMANQKEIPLKEFKLVEHYKLNSQIFKTNLQTIFSIVIDDLQNTFNKQIMIEERSILEPKQLTQIDEESQICFTENYNEISQEYSKTHRDDIKTEEAPLVNQESKQVQKIPLAKLLSFDKITSLSVANSPRHSRENSLTTHTFRQSNKPIQQLMLNANNGSKKQTVAQLAKNKSLELKSIAETLLAQSKISPKGNQTIIFNNLMSKMSNLKENTLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXA0</accession>
    <name>A0A8S1MXA0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0680099</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXA0"/>
    <dbReference type="OMA" id="KNNEWAL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="532"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="684"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="744"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="691"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8082681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="878" mass="103728" checksum="2E09B775E7DC920C" modified="2022-10-12" version="1">MQENREIYDSIDLCSFSPKLSDSFRPQHIKPDFSNAPIKNNTGSIGSVQFPSAEIQLLHHQTANPRGSIHIKKLNKNNEWALINIKKETIDPQKLQKNPRLSKMFYKEALIRRFVDKLRKQAYILPKILSNKMKNFLKENYIENKRISNQQKIILSKSQQKQTLSTLNPTGQLMLFWNLFRALQLMFLMWWLPFKIAFKPNYSFETIETILIYIFIFDVLLKLNQGYIMEGEFIMNRIDIIKHYIVNELIEDTLYITTQLFIIYKIMIDISIFFELFVFLQFIMNFIKLKRKIHKFEETFATKTSYTQFADLIQLIITVFYFAHFMACIWYYIGVESLKNFDSSWTTQTQFLLSDSGSFYLYSIYWATATMVTVGYGDISAQNNHEILCATILMIFSTGMFAFAINQIGEIFSTIDAEKQNYKRTILLINDYMQKNQVDQQVQSRIRNYLQYQEQIKLETSKEQLDSILQKLPQNIFTDLKTNIQSKIMQEISFYKSNFSKNVIPIISQYLQIQSLTPKEIIYQQGQLDDCSLFTVWKGEVLIIESQSGKILATLTKGQSFGEVEFLTQQNRQFTAISKDLSQVLKISREMFLKIIKYSDYDFEQFHKLKDQYLFEKIDQISSCYCCSENHFILDCTICQYKPNLEVLIRKQKAQDVVSIRRKFQRTIDKKTFFQFQTLQSNSVEKEEEPSGQNASSSVKDLTQPERSPSRRHKTRTINIKNIGQLLHIEDERVIDVPSFPWSNLDIQNEQQQQQQQQQQQQQQQQQQQQQQQQQSQNKLSYNQILELPSLKHKHSLSNHQIQIPSLNQLEQMAQEYIKITIQDFEKIQNYQIYFPQNNLEVVLLGQKKQPKIKKIQVAETLGDFTRKRVMSENFMNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N185</accession>
    <name>A0A8S1N185_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">KRR1 small subunit processome component</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">KRR-R motif-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0640193</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the KRR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N185"/>
    <dbReference type="OMA" id="TPDIDKW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22393">
        <property type="entry name" value="KH-I_KRR1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22394">
        <property type="entry name" value="KH-I_KRR1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000011">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000014">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041174">
        <property type="entry name" value="KRR1-like_KH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048550">
        <property type="entry name" value="KRR1-like_KH1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048548">
        <property type="entry name" value="KRR1-like_KH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048549">
        <property type="entry name" value="KRR1-like_KH2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024166">
        <property type="entry name" value="rRNA_assembly_KRR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581">
        <property type="entry name" value="HIV-1 REV BINDING PROTEIN 2, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581:SF0">
        <property type="entry name" value="KRR1 SMALL SUBUNIT PROCESSOME COMPONENT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17903">
        <property type="entry name" value="KH_KRR1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21800">
        <property type="entry name" value="KH_KRR1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006515">
        <property type="entry name" value="KRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="K Homology" evidence="10">
        <location>
            <begin position="128"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="239"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="278"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="239"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="278"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8080844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="346" mass="41044" checksum="06957CF8F0A84648" modified="2022-10-12" version="1">MSEQQEVVKNHKKYRKDKPWDNDPTIDKWKIPEIQPGEMNGSLLAESSFATLFPKYREKYIQEVFGMVKKSLKDHGIRAELNLMEGSLTVKTTNKTWDPWAIMKARDIIKLLARSVPVQQCLRLLEDGTFCDIIKIRSYTRNKEKFVKRRQRLIGPNGATLKALELLTDCYIMVQGSTVSVIGNWKNLKTVRKVVVDTMQNVHPIYSIKELMIKRELSKDENMQNENWDRFLPHFKKQNQKKKKVIKKKKEYTPFPPEQQPRKEDLLMASGEYFLNEKQRQQKKLEEKKKKQQLKKQQQQNQDGEEEDQQQYDHGEQQIQKNQPQDIEEIREQIQKSGKLKKVKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N494</accession>
    <name>A0A8S1N494_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0800120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N494"/>
    <dbReference type="OMA" id="QQRWIKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="36"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="159" mass="19475" checksum="A14303E14ECC0575" modified="2022-10-12" version="1">MQKLQQLQQTQLMAKTKKQQSEEGRKKTQEQQRWIKQQETQNLKIKKLELERQIQQQRESELIKNIVRYKKLKQQRAQSQMKMRIRQNSNEELRNKEYSQLLEVECQKRIQTEQSIRKLEQKEQLLIQELQTSQQLSSTYSRQLDPRNITKIRAKSYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ND50</accession>
    <name>A0A8S1ND50_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0790188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ND50"/>
    <dbReference type="OMA" id="CIFQSDR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="390"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="47692" checksum="BCB42D59A15BB12D" modified="2022-10-12" version="1">MNSQILIIESALLFVSVIALFIFKEWECNQKIIKLLFSISNISIFLLEIKHISESNYVLIAISSFIMILFSYKELKKEKNEIPQIQIINTQLSERNNKVDQEIELPNQLPVIPSIHESNSSIKDDNNKIQSNFQGEKAEQQNIRQDQGMNYLGDNKRPSFQLKNNVPQVLITRQQSVAQIQPNLNVIKFQDDFGEIKFGILKTLKSVAYDQLGREVECIFQSDRPLTKLLSTVEQTNQPRMQILYESKVQQNQYVDDTLRGAIQLDDLEYDEKKKEKKITWQKIIKALILIIILNLNFIIFDNWKLLQLILIYFIMQRQILQYKKLIANQLELKCEIRDILIWLVLIGLPIMIVLVINLIDKTIGRQILQMASWVSLVITSKYNKHCQDYFYGCLCYILVLTIVLCFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NE61</accession>
    <name>A0A8S1NE61_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cation efflux protein transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0800157</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NE61"/>
    <dbReference type="OMA" id="GIQNLWT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006829">
        <property type="term" value="P:zinc ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040177">
        <property type="entry name" value="SLC30A9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13414">
        <property type="entry name" value="HUEL-CATION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13414:SF9">
        <property type="entry name" value="PROTON-COUPLED ZINC ANTIPORTER SLC30A9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="6">
        <location>
            <begin position="47"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8088241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="488" mass="56902" checksum="41A7FE77205BE1CC" modified="2022-10-12" version="1">MHKILRSIKSFKGSRFANKLPTYLERLQQQEEMDRKTNLDVLRREVFNDSIQLPIKFAAYYITNQQTFWAELTRSMIDASNHLILFATNYINYGPNKQFPYGIEKVKHLVALIPSALFLYFGASISYESFLNIYNYGHYVGEIHGNAWGLALYLLSIGIETSVVFKNIRDAQAVDSNNETKTGLFKQFLSVFQKKDPVLQAILYENAITLGSTLIPLLSSAFTLIYPTHAFEIVGSLGIGLIQLQLAYHLSAQNLSSLMGEQSISDLEVKKILEIIKQNQYVEKIQNEKAVMLGSRKFRFSAEITFNIKQINQDTESKYLERFDKVIYSESYRDREEYLQELLREIQEYVLASLHIQIQKIESAIRNEFPNCIHLDFEINNKSLAETYSIDDGNWLFRETLLKSPFKVEKWMLDEVDRLIKKISKKKNETYIETYQSLCSSIQYNQWHDVEDDDESLKIIEDYRFEELQNELKGIDQILQFKNKSKGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJE3</accession>
    <name>A0A8S1NJE3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0770085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJE3"/>
    <dbReference type="OMA" id="ARYCISW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8086764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="144" mass="16684" checksum="9AEC2FFCAD7EA89C" modified="2022-10-12" version="1">MEYFLTRFTVYGNPFYNLNAGRDHTDWENQSRLRKALSRFQQSEEGQALPRRFTDQQVKGIRWGLPVVGLFLGRFIGDIYCAQSSIYMKGIFAVVGLTVFNKIGMDVMNDNEISYWTSNFPKLPSDIQKALSYGDARYTGRWID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NP51</accession>
    <name>A0A8S1NP51_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0880020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NP51"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8091343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9948" checksum="87934BCC8FD78F07" modified="2022-10-12" version="1">MHLAIIQSIFGPIPQYMKQRSKFKTRFNSLPKAQPQKQLHQIIDDQDLLDLLNMMFQIDHHHRINCKQILEHKFFQKLFSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPK6</accession>
    <name>A0A8S1NPK6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0880113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPK6"/>
    <dbReference type="OMA" id="YLDCKAE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011893">
        <property type="entry name" value="Selenoprotein_Rdx-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10262">
        <property type="entry name" value="Rdx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="189"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8091523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="288" mass="34934" checksum="469036D0B7CD4C1A" modified="2022-10-12" version="1">MSEEQQEFEGQEGQEGMHEEQSEQQQQQQQQQQQQQQQQNQTQQKQKPVVPKNDPPQEGAIIFDFEVCMDCSSHAWCTHHDENKYTQLYLDCKAEIEAQISKSYCTYNAKKKPSTGAFEISHQGIIIFSKKNSQVFPQPKLLPERIRQFLDDLENKRDVNKWATKKEIKQEQPRQIKERPTFQTYMKQREEQERLAKEQKQKQEEEERLKQEQEERDRQERERQEEEQRKIEEEKRRQEEQRQAEEKAAAEEKERQEQEQKKLEQQQEEANKEQQEQEQQQEGEKQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPX3</accession>
    <name>A0A8S1NPX3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0820049</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPX3"/>
    <dbReference type="OMA" id="RHISIRD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000051">
        <property type="entry name" value="Protein phosphatase 2C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="3">
        <location>
            <begin position="121"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8088724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="401" mass="45244" checksum="6652CCA1B5944372" modified="2022-10-12" version="1">MHQQSVPISRLQKEYRLKPLEFLSPQKERIVSRYPESTDYNSNNNGKYQTNLSLPTLPPLKKMSKIEQTVSIYKGKAARLPSIQFTGSQQQLQRKKQISEIDGMRKSITPEKRMGMRNSIIFAMKTKAGCQPNKATKINQDAAIVCPKNVESIGFKLFGVCDGHGLNGHIVSNQIKQQLPKHLSRLLKDGENIENQIQKAFTITNRELWNSEIDTNLSGSTTISLLINKDHIYSANVGDSRAIMCRFDNGWKVVPLSRDHKPDDPEEKQKILEAGGRVEQQKDLQGNPIGPFRVWLSYIQAPGLAMTRSLGDKVGAQAGVTAEPEIKQYTITEQDHFIVVASDGVWEYLSNEEVMNIVVPYLEKDNPDQAADNLIIEATQAWRRNSLARDDITCIVIFLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NS79</accession>
    <name>A0A8S1NS79_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1000103</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NS79"/>
    <dbReference type="OMA" id="LLHDYDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="452"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8096117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="605" mass="72082" checksum="63D8FFB337C8F7BC" modified="2022-10-12" version="1">MKLNTSTSSAPFIPQRPVSQNSSFVQGRMQTPPRVARFADTSPHASFEFEDLNSLSLEEINRELDQNINEVIMLLNRRKSLILNKKRRLKEEFSNKIAELKRYQYEDYVQDLQRIEDPLIQSLMQQKSLPKMQKLLGNVMRDQQDRKVMELSKSQEFWQHNKNVETEIDNLLKKLKYFDNELEKDNNEIYEICRQLDSIDDNIRDLLITKVDDNVQRAEPLFANKQRQQSILNGKLNQRNKLKDEKMNLIYKIIEELQRLRGLRQERINKLNIVFGDEENISNQNFQGLQNVQGSQQNLDQPQPSKLQAYMDFMEQFDNIAQMRDQISKLEQAEQALLNDLDRLYRESDQVFSELIRRISEEAKTFNEWTENLKKRIRYIKANEYFFSQTNLPEQQNGLLNSLQLDLKEVSRKFKENMNYLKPLIEEDATIRAGLEKTGSTIQQFKNMILAIQNEKERRRELLQILENCARLRQEEDMKTEQLRNIELEINKKLQQKRNLEEDLDGVLGDADLNTFKKIESLLHDYDQELVHLNNQKRKIMKEIEEINQSVINLLARIKSDYDDILREILPKDWTNTYDLKKITDLKRMKNYLKKTFKRFGIMSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NSF8</accession>
    <name>A0A8S1NSF8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0900064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NSF8"/>
    <dbReference type="OMA" id="AYQNICI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="531" mass="62865" checksum="970553E1708A8233" modified="2022-10-12" version="1">MSSAVTRAGQKWKEFWGQQITKNTQLEEKKSEAYELNNYFQMAERTLITISQCHQQLQIQYNQCAMIVTKIYEHEIGSNKLIANNLVKMFQKISELHGEKVTPIYQLKLELKPWFDTMEQLKILLERYQEDRLIYDHYKLKMKELQNSKDSDKINRNERKLEKATTAYQNICIDMIGKMDNIISNKIRMMNVLLSSIISIEHDVSSQESKLYHECLDFDKEFSNGNQTKSVQPENQEQENNFINKTRKLLNLQPALDSKQNEKQHQIKQPQFGETPTPNPDNDPFEDFNRVSAQFAVELKDDSESPALCKDSRTSKQNQQQMRSQIQQQQEQFYQTIKNQRKQLEQSDIKQSNQTDLNQGFPNPFATPQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQDFRLSQSMVVQSNTGWNPFDEGQNNQQSFKKGIRNPFDDEVDDTYVQQQQIQNPQLQIHSQILSSRSNNPFANETNQQVPPRMFQTYQPSNPFADHSNEYQKQPNQNISKNPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTB2</accession>
    <name>A0A8S1NTB2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0920170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTB2"/>
    <dbReference type="OMA" id="REVQDDH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035833806" description="Transmembrane protein" evidence="4">
        <location>
            <begin position="18"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="531"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8093196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="67735" checksum="A986FC8CD16DF5ED" modified="2022-10-12" version="1">MLLILLVGLSCIIARDADLIETEITQLEHLIQLQTEKLHYLKELREISINNQQNIPQIDQQVLQEVVQKKPYKTQNNGKHNLNKERSFTNRMLKKYQFNITNSILDASLIQTFQYKSTTTDNSFTQMLYVVTNTKEIEIYDLSNTMIVNAQLDFQPQYVSISNRADDPILALANKDGQFALYKIENSRITIESQDSQKPKTKLVIQLNKEFEMNIENQQITSMIYAMVKGTKYILFGSSSGSVYAYTRNGTLVGERNFNSPILQVIKSYPNMLYLTSTGFGYINPINLEIVQPICDNLPFQIVHLTLDIQQTNIVYALSDLGEVYVFEIKQNEQCKMKLKLVNNQQPQLLLNNAKLYSLRHYLIVYDQNTRESLLYNTTDVLTEVDYDQPFNLEFFKQIKGQQVVLQSIKGNGASHYLLSREVQDDHELISYYEITMPQKKDTDIFSNFRFPIIIIAIVIVLLYQFWFKGKKKDKKEKGSKKKIRGEDEEIEQILNQVKKPQTGTIGSTQTINNRSPTRSEVQNGQKYVKFDENLKKADKFKEQLENLDQKTKLLNERVGVTNNIEAQRQKLAQQALLQKNKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUR6</accession>
    <name>A0A8S1NUR6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0940140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NUR6"/>
    <dbReference type="OMA" id="LMGNNCT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="37262" checksum="9F901DF38A9A6AC7" modified="2022-10-12" version="1">MKNFQTNKGLQINVLTLKLMGNNCTCWKYDLQNQIEQDRKSKPKWQSLEFLKSQEHKIVKMQSWIRGWQARQRVWKIQLQKYNDSVNEQLKTYSTSVINVGSRQLPPFDFGIDDINDSLVHQKVLKAPMKLENGATYFGEWINDLRHGKGILICDDGSKYEGYFYQGNAHGRGRLIHSNGEIYEGQWENDEAHGQGTYIHEDGATYVGQWEHDLQQGKGSEKWPDGRLGSFQSLVILKEPINKAKKKDQENLYGQMEQSMMENLEVIIQMVMGNTYGRMVNNIQVIGKITKKMVKVSIYGLMEKHMREILFLIKNMVKEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NV11</accession>
    <name>A0A8S1NV11_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1000069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NV11"/>
    <dbReference type="OMA" id="LNEEEHF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="253" mass="29926" checksum="8682CC003474EC79" modified="2022-10-12" version="1">MKQLSQTSQSFKHKQKSEPSLLPNIDILKENVKSSFALGHLRGLLTYKQVRELPKTEIQMRRDQTYGKANKKSKNKRLTFLKFGFRTHEKKNNYYLQPLIKKVKDAFGEVANDGSMGVVDLWSDRNMNRLMDAIQMTRDNLIVRKLGKELNEAEHFLMIRMEEKKMRDRIFMESLKLQFQETDTFSLKFQLSDKDKKIVQRVAQPTISSNLRYNNPNENIQTIVESIDQLHQENLEIYTDLYKQIKSIQKHIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZM9</accession>
    <name>A0A8S1NZM9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1010012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZM9"/>
    <dbReference type="OMA" id="IFATRNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="243"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="288"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="71"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8096281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="512" mass="60047" checksum="0DB0E910A5638A9A" modified="2022-10-12" version="1">MLQPKMIELENQLNCSQNHNQPILMVKFDKEIKMGERLLCAHCLENFEQESKLIGFKKTLKMIEEKQQSKLDNIQELIKANQEMVESLINQIQKLKSDLNQYLDELLSYSIDWIKNLNNIGLASSSYSFLDELELLINNPKDNLINLESIIQQLKLLNNSLSAKINIKISNLKNQDLYSQCEKILSNLNFFPHLKLFDESIKQEDQCYAISFNVSDSLMISASGCQIKVWDFVRGILNEITTLSLHTGPITCLQFSKKNNSFLSTGFDKSICCWNQINDKEWKNSQFYKQHTDCVDCLILNKSEDQLISGGRDHSIKIWKVDFLQNQLYYLYSLDKHTNGIYSLCLNQSENTLVSTDCFQIIIWKKDNKQKWQFENQITQNIQFIGSRLYFINNQQLIFATRNEVKNYISILQLENGQQFNEIVDLKLIENNQIMDVNLFPICYNKEQNLMIIKHKLNVYLIKILKDGKLEVLTEMNYSTIFIFGKLINDGKYLIIWEEARKKFDIYELQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P169</accession>
    <name>A0A8S1P169_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1020028</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P169"/>
    <dbReference type="OMA" id="IRPCFEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00878">
        <property type="entry name" value="Arf_Arl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001915">
        <property type="entry name" value="ADP-ribosylation factor 6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="128"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8096646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20622" checksum="52D0F0814870A86F" modified="2022-10-12" version="1">MGCLTSSQKEEDISIALQSSKKLKIVIVGLEGSGKTSILQYLKNGKFTESQPTIGLNVETIQFKARHYLIFDVGGKVRTLWSHYYENLDGLVFVIDTTDSERIEIVKNELKKLGSEIKQKIVLLIYLNKIDLPRSSPVELYKDIQQQESEVIMQRCSAKTGEGIWEGIDKMNKLLDHKFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1G6</accession>
    <name>A0A8S1P1G6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1020001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1G6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="25872" checksum="04DBF28282DC8B10" modified="2022-10-12" version="1">MSLYSRKFFKHEKFLQTILEQSNQFIYIFLVIQFFSLSSLYNSNSLQIKQPPLQLIIPQINQHRFKNLLILLQYFVLLGKFNELQFLILLKLSLFFLNILIIEGEALSGLIDLYCVLGQTRRNKNCFFQNYMPYTSYLINALFSKSTFFFFLIFFTLITFKERVALEYFKSHLREYFCIILKSNISTNTFNQLNQLLWMAQKLIQQVSLLYTDNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1Y1</accession>
    <name>A0A8S1P1Y1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1020196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1Y1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035833548" description="S-protein homolog" evidence="1">
        <location>
            <begin position="21"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20959" checksum="9F8BE37F37107317" modified="2022-10-12" version="1">MLLIRLILIIEVFGWRQYNSQSFDSLGFAIDNQNCPKLHIFMAGLHPYKCVSYALICKGVTEVQVKTGQDGTQHQCHPKFQPWGAGTPTLDGYMVHCGFNVGVSLVIEQLPDDSYKYYCKYFANNSHNKCLITAYQKGLFRCQMCKLPFFGNGFGCDQVLWRYDRRFIVVCKKITINFIKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P9C1</accession>
    <name>A0A8S1P9C1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">60S ribosomal protein L39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0240123</name>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0350107</name>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0380228</name>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0510220</name>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1100086</name>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1270043</name>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1510035</name>
    </gene>
    <organism evidence="8 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8066020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000156">
        <property type="protein sequence ID" value="CAD8112358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P9C1"/>
    <dbReference type="OMA" id="RRTKMNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein-like L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00629">
        <property type="entry name" value="Ribosomal_eL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_eL39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020083">
        <property type="entry name" value="Ribosomal_eL39_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970">
        <property type="entry name" value="RIBOSOMAL PROTEIN L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00051">
        <property type="entry name" value="RIBOSOMAL_L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8056064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8063723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8066020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8073746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8099679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8105381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8112358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="52" mass="6565" checksum="A281982F7CA8D221" modified="2022-10-12" version="1">MGAHKSLRMKKRLIKANKQNRPLPNWFRYRTDNTIRYNSKRRHWRRTKLNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBT9</accession>
    <name>A0A8S1PBT9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1130033</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YIEYHTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050505">
        <property type="entry name" value="WDR55/POC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019:SF20">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2456"/>
            <end position="2498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="504"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1056"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1235"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8100657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3149" mass="371066" checksum="D0AA5C0AEE6CDB52" modified="2022-10-12" version="1">MNNNTIYLRGGGCGTSKKDPKQHKQEMERGKQKVDMTEKEKMAKLIQQHSEIISKNAIIAMSEKKKLMSSFQFFLDNRQELWGIIVDKEESAQIIDIILNNLKPLLEALRTLIKGFVAYSVYLSQIIQLLSWIIFCFYSNSKENKERFMKVNDQTQYLEIINQIKKEIEIEKNIDKYQNNLEYELYIIESIFTIVPTDSDEAKEIAASFLIGAATSFLSFSINDEFLTSLKKGVIYIYNEHDKSSRKKILKIIFALLQLKYETINKIQKSDQAKNCVDLDQLNNVYNDVISKSSDWQVWCTWTKVLSQLFQQKLILPLDKIQSLKISNEPYIHKNEIRKTCWLNIDSRLIENNATAKLIYQNIEELQELGRLQYTILYGYKQMPSFESLYSSKLTQAIEKENFDAFQFNLSSVSIMLHNSQEIQKAQEQLQQHIELLSTTNKSKIQDIENYLKKAINNTIQLLESINSLHEIEHPNDNELESPKQPVGQNAIFQSQLIQNQSPIQQIEDKEEKWLEEIVKLKKSLSIKFVSFFLKSLLIKIKNQSKENQQKEIDDKTKEEYQIDFILGEKKQDKEQINQNNQVQDQIIQNNNQDQIIQNNNQVQNQIIQNNNFDQDQIIQNYNQDQYKLNVLDLLQEIEKKKVEKKKTKTPKYKEIVLIFKKDPFKIENLQQLQSRLKYISIKRSKHQMKNDEEYKFGFKEGIKLLQGGSAFLRWNLKLFVKMKFYIQELSQNQYNDKQLNQQANQIIDSLKGFSLQLASLKALQKCIKFFYSKYDACIKYKLIRKVSQKRNVIYQVLKTITLKKFETLNMVYAIHKETIRKVKETGVIYICQSIFQAIQNEHEKFLDDIVQLKRAIQMIIELLNADEKKNNKDQDEFKIFTNQEIFNQVKIVNPVNNVQWLQNKDFYFKLTLFYQNYCHSFQSWQKKNKNYSQNVVQQIQMKQQLIKEYLEKIEIAQEYKTLQEEDDQFKQELYNEVQSHLESLLKSTIQSEQENFKAEQISPKENLHFFQFLFDNIKEIEHQVQVQFDNLKFINQQQGVFSTLINNSLLLKRANSQYQIKIEQNEKKIKDLVGNYEEKLPQQVIELKDLQQRFYDQKLILSPNLKSEIFFILTQIWKKEDVTSDPSYYDKFAEAASDLINKNKWRVRQSCIYELMYFKNSCLTQATVDLAKGLLVKCRIYETDRRNKQLLDDQDENQVVSMFQQCWTQQEEDVQTKIKQKLEELNDIAYKITIETKQYEKSKFKKRYVELESEIQGIVKNAESLGNSLGTSLLFFQDIKNDLVNIQSQLKNLQGSIDQIHKDIQLLQGRSIKDLLIIRMQRVLQQRIILNSENVYVPIKTKEKQVLENQEDEETPLYTDDLFSNGEINEFIWKQQKLSLLIHGQAGSGKSTAARKIEEFLWLLFKKNINIAESIPIIPIFVSLPQLKDPLSMAIEETLKSDNYRFNDKQIIEFKEAVEQNQFKLVIIMDSYDEIKSEFTNRNLILSNKLYKWRCQSDEQRFPKIITTSRSEIFINNEYRSWFLPESENLQYYKEIRLLNFTNEQVNTYIEYHTILSVKKIIKDFYFNNKDNSGFFAFEEIFNEIIKVINEKEQEDDKQDQQFLLSKNQIRRILSLCESFVSGDIAMTMQQSLEEVWYKEYYIKNIRQMDIGSLLETPFMIEIVASVLPQMIKHRQELNTLKESFMKKLSQQENFSNEQLSKEWSKIISNEQFLDEYLKVSNDSDRDSLISKHFKKLDNFQILQNAMKLDPLCSYDFYDQFFAQYFKRQINKLIESGESLAYISNLSDLWEFTHRLANDMTLQQVSQVQYIPRILLFQKKKDVDWRDIYFNDEQEQGQIKKLFRKIMPIKQKYGIYSFNHKSLQEFLVAKWLIQQLDQFNQSLNSNGIDNQLDDLCVHNLSKDSMNGVLKFMVDKLNQNQFLQKKLFEITILSKNIQKNDQNKIQGSMKVSQTIQLKRAQTQLLAQQNFTQNLYTDEEVTQRIIQASSNCLYLLHLLKYPLNKDLSYIKINDIQLLDSNFVGANLLKSEFSNVNISQSNFNFANLQDVVWNDILIDELPSIETKLNLPQQSFYFNYIKSEDLLIFNTKDKIVSFNYKTQTEAVRKNESEYNISNVKYILVSQNQSNQKIAYFHNDSITLISTINSKIKLPDGFIFQTAYFTQKDDLIIGLIIGSEKLLIRLATDVQQEEIVINDNNNIYLNQLVLKKIIQIASYENDIIFLSDRDVIFIKEIDNKYIYFQFQIKCSHKKCKNQQIDENNENQYECQLLQKYTCIEVSENQNLLLFGYLEEDDYKSSGILVIEKQYLTQQPQDKNQEQKQYITTDKFIQLDIPGEDQLKAYFVDEDKYILSFSYSNNLTYMKLWNYKEKKIISSTILDGQIKQMTKISGLLISTINNNGKIQIWDLQALLQQQNSNPKSTITCCIFSTDRNIISGNQHGIIQIWDRQKGTKIGKDLESHTAAVSSLVISQKENEILISGDTQGNINFWNLLKYELFKSLYIISPIKSLNMIEIEKNYQLITHSQNYHYIQLWDKEFKSYQLFCENQNNIKINQIELQSIIQFLQNKSTRSSPKKYADSTKLKDCQAFAIGKYTNNFYFAQSLLDENQQQTKDILINIYYEEFKEQKSQDETKEQLFFSRSVILEFEKNNTETEIVQIIKEINQKKILVTTNTKIFIIKISKLGIEKKRFDYPNIGKILDVQIDEDLLFCCGNSVIIKNIRSGKDIPSSIKTINQNEQQIEMIGEQDFFCSLLYYRKKNTLFIGMTSGKIIEYDLLQCNSQVHYQHKKQIIYLQIHTRQDGLQDVLVTAGKDSLIYFWPLTIQDLKQLKPTDQKQILLEDSEINKFWYFDQENYFICQNQSGYYLAAYFPDGLSYYKISDYDPTSICDYNNKKQAFYQLTFKKNSSHDSSKKAELTKTMVLQEAIKAHYQENNNYKAEFVLLDHNKSQIITADDDGNILFWSPNGDHTDKIKPFDTDQALQVQISISQKFNSIICGCFDGNSKKYLIAKISLEDQKKQDIIIAFGEDKPTLKQLQILSQDLILILTGGSTNNINIYSIKAEQFIQQMKNLSIGSAVLTEYGQNKESVFLTYGEFNQLTYYEKINEKYGSRFRFGNAIKKFSCHYANITNSKFLSKHQIDLTKIFNQGDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PEP5</accession>
    <name>A0A8S1PEP5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1160046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PEP5"/>
    <dbReference type="OMA" id="YYPYSYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="18474" checksum="6683D59C43793E4F" modified="2022-10-12" version="1">MFNYSGMLPKSQSLYFHPPLQVLSTRNIQTEPEISKQQHKPHKKYSVITDEKRNFLIKQVLEEGVSVKLAAEANEIKLSTAKAILKTYKTQGRIGKKKERQKRPKLNRKTVDKQLDQATLIKKQLPKLGQNFIYYPYSYQPPPLFYKIEHSNDIKTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJ02</accession>
    <name>A0A8S1PJ02_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E2F/DP family winged-helix DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1180100</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E2F/DP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJ02"/>
    <dbReference type="OMA" id="NDHIAEP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000360">
        <property type="entry name" value="Transcription factor Dp-1, a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="3">
        <location>
            <begin position="43"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8102418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="27267" checksum="653699CFC3715663" modified="2022-10-12" version="1">MKRIQRRLSKCVDEEMQSDSSKQQSDSEFIITETSESEKLKSKSFWSLKGLSYQIKTLVKELKSTTYKILANILIEKLQNELQKIHTTERRKEIQNLKRRVYDAINVMVAMGVLEKDKKQISFHEQKDKEVINFQKEQVRTKLEILKEKRKKLTKATLTYMMYKQLNQRNQQIKMESEQILYTPVFAFSVQVFDNDLCMQQKNGKKLIIKSKQPIYIYSELEVLQKILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJ52</accession>
    <name>A0A8S1PJ52_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1180140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8102498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="346" mass="41110" checksum="A727728301938065" modified="2022-10-12" version="1">MKSSQTNSNLAQLQDNYNKIHVLSARTNKLGLKSYMPQKETRQFSHHVDGIQRKQIKVKNLLVITDDQKLFHKVQESIQSYRSPKNFQTIEQECPNHLDNGNKKNSRMLSPKEQNEMIEYLQKKNEELVQENKEKQQLINRLLGACNGTQKIKKMQSPKSQSIFQQIPKSADARNKMDTDIRLPLIKTPEPFLEQRQDIQIVQNENQRNDLLNLYNMSFGKQINLNEEKKEQLELTSNKNRQTNPREKMYSQPNQPLKKNHXNIVFILNLIYQYKSKRQRKYIYESNHLGKSRLVILLLKYCFYLNVNFSIIYKQVEIISLCRNEEFLNISKYQTNKKQLHLQQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PK81</accession>
    <name>A0A8S1PK81_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cilia-and flagella-associated protein 96</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1210157</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CFAP96 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PK81"/>
    <dbReference type="OMA" id="HEPSLFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029358">
        <property type="entry name" value="CFAP96"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31144">
        <property type="entry name" value="UPF0602 PROTEIN C4ORF47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31144:SF1">
        <property type="entry name" value="UPF0602 PROTEIN C4ORF47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15239">
        <property type="entry name" value="CFAP96-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="260"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="295"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8103657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="310" mass="35832" checksum="008A92EB6F3363B0" modified="2022-10-12" version="1">MINIFVIQRIFLKENGMDYSHSLLLFQLVINITILKKVLRKCQVGKGNKKGVLINQKIQNHHEPSLFMNPGYISIGDPYKDPFKAKQIYDKEKEMAISNPQSFKPTDKSKSIKHSEFEHLKEYDDKKYNTRKASGEVITQARNFQTNPAKKGLGRRTINNLFGNYEYIPDPYDRQEELDRSERLKYKSKLLPGTIRFVSTSHGNRPFTSDGDLLDGAGYIEQKVKRPRYKGVPFRPSNKNKYGFQGTFEVFQYVEEGAPEPKTKQNTFSKLLKTDTYERPWRPNSNGTHARPCPSVSQKLRNRSGGTTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKZ7</accession>
    <name>A0A8S1PKZ7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1210071</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKZ7"/>
    <dbReference type="OMA" id="SHEGMIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000042">
        <property type="entry name" value="Protein phosphatase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="3">
        <location>
            <begin position="61"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8103484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="320" mass="37192" checksum="193A0C5377CD3EA9" modified="2022-10-12" version="1">MSLTQTNNQKKQKPKLQLVVNVPDVQPESTHSSDGWKQQTDIFFNEPIDQSLQFLHNGKTIADVAQLKCGRKYQEDRFVAIPNLNKNEDLQFFYAIYDGHAGNSVSAILENKLHEYLQKDCNFEDNLEKAIINSFEKMNQYILDCQEENQHLGGSTALCVINKQKDLYIVNLGDSACVLITEEYEIEKLNQEHKLNREDEFKRVEQIATILDRHSIPRINGELAVTRAFGDKKHRQSGLIAIPEIKLHKIRHNDKYLILASDGFWDIIKNEELQQLIENWNRQEIDQLAQYLLDKASSKNTNFKKDNMTLIVVDIQSYWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PL83</accession>
    <name>A0A8S1PL83_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1220168</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8104055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PL83"/>
    <dbReference type="OMA" id="CVGLPRE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="23"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="316"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8104055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="450" mass="52614" checksum="245E0091C481E57E" modified="2022-10-12" version="1">MNTNHSLKAPVDSLLGRVVCKNYLIVSKINEGSFGKVYKAIHQIKQDYYAVKIEAQEHRQKKGETLRLEAEILKKLNGEKGIPRLYYYIEDSTQRVLVETLLGKNLDELYSLCNHKFSLKTILLFMDQAISRLEYLHSQGYIHRDIKPENFMIGIPPNENLIYLIDFGLNAPIYDSQKKLLPKLKGQPLVGTARFTPICSHQGYSQSRASDLESLGYLAIFFLKGSLPWMKIEAQTKEERFYLIGQKKMRETPMSLCYGLPREFEGYFNYIFNVSHDTEPSYNYLRKLLKGLMHRMQYNDNIFDWQEIIKKEEESKKSSNKKIKLNAQTQQFKPIESEDEQDSPKVAMMQSKDSKKSSSNFLKRSITDQLNFQKQQSINQRNNIELLISQNELLNLRLKQKLLSNNNNNNTQEEPENFGFGIMNEYYPYPTMQQQMDQLITIITKFIINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLV9</accession>
    <name>A0A8S1PLV9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1230045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HEMIRTN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23022">
        <property type="entry name" value="zf-HIT_DDX59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046455">
        <property type="entry name" value="Sec7/BIG1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF375">
        <property type="entry name" value="LD29171P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20252">
        <property type="entry name" value="BIG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="1">
        <location>
            <begin position="549"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1599" mass="188054" checksum="429DF6E4934C109C" modified="2022-10-12" version="1">MNRQYVLPKYQEILQEYMEKVRKNIPKQYPSFKDFLNKMIDLVKYESLPKLGKHNVVFRLTINMRQTKLTQLALSTLFRLVSNGFIDGRIEDTSQEFYTDEFSELFNDSNYNKGEKRTIMDVLINSVTSCLFDKDEIIIQGLKLLVAFVLNPYCYVTSQNLTRIVRTIIFSYTRSRTQTVDRNTKNMLYQIINYTFQAVEGEVFTIQRNKRRMSIKEDSVQKLTQLVMLQLVDMVCLNRTSLNFCIQSDIQNERNQPAGYFGYCIMCRKSANLYCKDHRVPICSFICKKQHQEYVEQAQSTYSGAVKQFEENLDCALQLYDSLCNLLLNKTTLEQAKNQQIILECLLYILETPDFVLSKNEKFIKTTKERLCNQLLKYCLETEKTLYQFSFRIFQRLVNIMRKNIKHEMAIFINQIYLNILLSANSNVLHKQTALESLCSILERPKTGLEFYINYDCHTKHECLMSKIINTFYEIIVVSIYQKAEYQISTQQEILLKYLAIKALSYVIEGLNKIFDKFIITPSEEIGSPQMEDQNANVNDNTTVMYINPIEIQRQLKQEIMKGCQIFRKNPDKGIKYLLDAQIIQNDAKEIAKFFRENQQQVSKDAIGAFLGGHQQLNIKVLSEFTDMLKFKDLTVEQALRYYLDQFTLPGEAMQVDRVVQKFSDRYYKENPNSTFKSSGSIYTYCYLLVMLQTDLHNPSVAEKMKLIDFQKLARSINDGDDLPQEYLTQTYNSILKQPLAVREKEKSRVFMKESLTQSIRKKQDLFQREKEALLKQGSELIKTKQDSHETLYQIINQDMAYLIKPFLECIGKQSFEMFLFVFNNDQMEQASNQCIQGLVLFIKLCSFFSIPLQDYMNTLIKATRLNNQGQISNKHINLIKQILQTVPLIGNGLRENGWKSILKMISRLDEMRMIQQSKDNQDGQNISILPELLLESDLIDKIFVQSKQLDDEAIQEFINALCFMSKQEIYQTHPRLFCLQKLVEVCDYNMKRVSFVWTKMWNIVKDHINEVAVKEKKVAMFTVDSLKQLSIKFLQKDELYDFQFQRDVLKPFETIFLQSNLEVKEFILSCINHIVLNHKHNIRSGWRMIFGLIALGLKEENDKICKIAFQILSQIMEHNLDLLQDVFIDLIQTLKVLAGKNQEDMAIASIDFTIKCLGYLSKQAQIIPKLNWNEFEEPEATVRNASTAVQLEKIWIPLLGVLSQLAGDKRNKIQAKSMEALFETLQQFGYAFSAEFWKMVFSTVLRPIFDEIQFTFQQNYTVANTNNDWFKDSCKKGFSLIINLMKRYFQKLRGLLPEFLKLFENCIQNQNVKLAKYSIHSVKNMTLKIGMMFNEEEWEQIIQFIDRMIRLTTPTKLSSFANQSLTSSRMRNMIEDCFTQCTSQLLIIQISIEILQHFNQKLNLQQLQTIENTFLYSYQFAVQFNSQIEQRYLIWKQGVLQDMKFLPGLLKQEREAYGCMIIIKKYKLKNHNILDIDSLIQPIDVFIKKHEMIRTNTQFQEELDQQMQKLKLQEIERETQNYRQLLDTYILPTLCELNVEHLKQYNQQLFLILLKTLSYSNDSIICKECIRCQKCLQQEKSNLDQQLLQLVQKLYDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMN6</accession>
    <name>A0A8S1PMN6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2HC/C3H-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1240056</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMN6"/>
    <dbReference type="OMA" id="PEQYNKP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026319">
        <property type="entry name" value="ZC2HC1A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049899">
        <property type="entry name" value="Znf_C2HC_C3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555">
        <property type="entry name" value="C2H2 ZINC FINGER CGI-62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555:SF36">
        <property type="entry name" value="ZINC FINGER C2HC DOMAIN-CONTAINING PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13913">
        <property type="entry name" value="zf-C2HC_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52027">
        <property type="entry name" value="ZF_C2HC_C3H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2HC/C3H-type" evidence="8">
        <location>
            <begin position="401"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="8">
        <location>
            <begin position="503"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="296"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="296"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8104537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="533" mass="62932" checksum="873E6FCE42B52036" modified="2022-10-12" version="1">MYQGSNLSRTGQMAVSNDQVSNQLKLLKAKMSNRDTWQQGTNQQLDGYPHTQPTKPSQSPLYPKSDYDFYTMKQQQQQQIQSNSQPKIIDPRTLNYQQLQQYKQQQVLPPQNPIRNDPYLGMNAINRLNYYDQLQKYKDDPYKKSSNLNTYNSPQSYYEQQLNKRQQQQQLIQQPPKYYDDILAKKQQTYGYQQNNNYDFTQQPISLQNKNDYMNYNNNNYLNNGLYSNNLQWQNTNQNNLYNNNNQNYNQNLQQLSSQSNQYNQYNLYGNQNINTQQNQQQSSQNQYNYNQNTQSLSPLKQQQASQQLKYSQHNSQPLLEKRDRPFERQYDPIPEQQKQIQEIPEQYNKPQPRKQQQPQQQPSYNQNNTKAFNPIDELPAVAKKNQTNQIPPPEDDDDGDLQECPEGCGRRFKENALDKHIKVCKKVFQSKRKEFNSKAHRQVNQEQAKLEKQGQVKDKIIEKKKQMAQNGDPKWKKQSEAFRQMISAVKQGGTVEIQPQDDLVECPACGRKFSEQAAERHIPGCKKRTFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PPF1</accession>
    <name>A0A8S1PPF1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1230128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PPF1"/>
    <dbReference type="OMA" id="CEGHNCH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035921086" evidence="1">
        <location>
            <begin position="19"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="257" mass="29938" checksum="79C26D8260489CEA" modified="2022-10-12" version="1">MIIYYIFGFLYCIKGALDYPTACDCQEHIDQDSCESHNCFWAESECRIAECNERSLQNCIIASQFLSQNPHYCFIKNGKCEKLNKCDDIQINQNNIGQAREQCQLYQCAYDLVSGYCFKPEKCNDIYDQFTCDSFLIAKSNPLEIDSICYWDRKCKARNSFIDNCKSIKDKDVCQQGGCQYENDQCQEIDCSSRSIKECNGEYVNHQGKVFVCYENNDKCEKIETEKLHKTVCNSMIGHTYKDKQCKKCNSYADHWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQ93</accession>
    <name>A0A8S1PQ93_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aurora kinase</fullName>
            <ecNumber evidence="13">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T1260110</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="13"/>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQ93"/>
    <dbReference type="OMA" id="KIMNLMF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="67"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="20"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 11">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="145"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="196"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="10">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8105202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="321" mass="37804" checksum="4C2E2863A1CE6749" modified="2022-10-12" version="1">MWQNTNSVLSNITNSYENTRGFDGLEKKKPAEDFIEEETRNNYSEEEDMNKDQTKEFDPNQWSLGRFEMGRYLGNGKFGHVYLARERESKFILALKVISKRQLNLCQLTGSLTREVEILSHLKHPNIIGFYGFFQTEKRVYLMLEWAPLGDLYGLMKKQTNRRFSEEMASKIIKQITEAIGYMHSMNVIHRDLKPENILCFNNEIFKISDFGWSVHTPSNRRKTLCGTLDYLCPEMINYQSHDNRVDVWTIGVLAYELVVGRPPFESHNEHDTKKKIMNLMFQFPNWSSKEFQNFVKGILQHDCNKRPSITQILNHPWITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PR38</accession>
    <name>A0A8S1PR38_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1280027</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PR38"/>
    <dbReference type="OMA" id="THHNGRT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001033">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="18"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="324"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="361"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8105625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="424" mass="49508" checksum="E8C735D1872F97D5" modified="2022-10-12" version="1">MDTPIGKDLTNQVIGKVYKLIKRIGSGAFGEIYLVAKGKEEYAMKLERSDTKHPQIFFETKLYNYLQGSDPSDKGIPRIYAQGQDGDYNYIVMDLLGQSLEELFSKNNKRLSLKTVLMLGDQMIQRIEYIHTKQFLHRDIKPDNFLIGLGKKATRVYILDFGLAKRYLTKEGHIPYREGKSLTGTARYASVNTHLGLEQSRRDDLESLGYVLMYLLRGQLPWQNMKANNQKDKYQRIMEKKLETSPDQLCKGFPIEMSQYLNYCKNLKFEDKPDYNYLRGLFKDAFKKIGFEWDYKFEWIKDEQLVKTQQDIQSENKLMIMTQPLQQQQQQQQQQQGIRSNGIESDKKIGNTNSHHFNSNTQQLQSGQRRPQQQTSKVSQQLSVEKKRTTSQNKQFIQSKELVKPVTQVLAPKIVTTKEPHRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PS25</accession>
    <name>A0A8S1PS25_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1270079</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PS25"/>
    <dbReference type="OMA" id="CFRYSET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8105453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="417" mass="49145" checksum="7FC31A145964A867" modified="2022-10-12" version="1">MNYLLILLYIYQSTILSLFTIIIPILLAEKGYTFEQQGMYSLISYPFYFKFLFAPIIDLYQFQFLGKRKSYILPINLLLSVILLYLSRIDILTNYHILWIFGFILCLFLGIQDIAIDGLATDLCKQQGESAALLQNIGFTIGNSFLGNFLFIALYSSQHCSLETFFMILSILGLILTIILYLYLFEQQEQYKRTTNFQDLKSVIKSFFQNENMITFSLLIFFERTALSSLDATFRLKLVQFNINKTYISFIDTIILPLKIATGLIYHQYFYKYDFKYYGNIQYLRLISTFFAIITLLLIDKFELDGPYSYIAIFLSNMCWWILFNITYIVRGNFQMKITTPGVEATTLTILNSISQLGVKLAFSINLIMANYVNYYWLLCAGWIIHLLYCFRYSETYRNLYMQPIQMWYLNKQIKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUR9</accession>
    <name>A0A8S1PUR9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">PA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T1300081</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PUR9"/>
    <dbReference type="OMA" id="CIITVIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02123">
        <property type="entry name" value="PA_C_RZF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000050">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056858">
        <property type="entry name" value="VSR_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044744">
        <property type="entry name" value="ZNRF4/RNF13/RNF167_PA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702:SF1">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25011">
        <property type="entry name" value="VSR_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035781480" description="PA domain-containing protein" evidence="13">
        <location>
            <begin position="17"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="14">
        <location>
            <begin position="56"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar sorting receptor thioredoxin-like" evidence="15">
        <location>
            <begin position="183"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF25011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8106358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="51812" checksum="0B98EF1683488A51" modified="2022-10-12" version="1">MYKILLLICIITVIQSKLKVLRPSNLINEYIDYSISNFGIIPFGHRLMGLVDLAYPRNGCSDIQPTYGAHFILIERGNCSHVTKVKNAEKAGYQMAIIGNYNDDPIESDITMADDGYGYQVTIPSIFIKKKHFTILSEKAKDYQIEDSNDEKIMLLLKFDVVQTDKISVIFGLNVQDGESFRIIDEYAPYYNKLEDQNINYTLVYFMMSFNSTILEINQRDSDCICQNRYCVFDPDGSGIATGRDVVQEILRQTCIFQKYPKKWFSYMDQFNFKCSKPQAYSVCSQQIMEIQGIPKKEIQNCFDESFVDQNTSLPTKNYTNAINILLENQMHIYQVAGMNVFPSVLVNAMSYKGQFSGKGIFGEICNSFLTPPPECSSEIEGYKPPVLEQSIILYVLIITTFVVLFSILVFCLFKKLIKRDGERVTQPQVNEMVSQYIKFYEGKDKQKEGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PWD8</accession>
    <name>A0A8S1PWD8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome b561 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T1320013</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8106849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PWD8"/>
    <dbReference type="OMA" id="WRIIFEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08760">
        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00322">
        <property type="entry name" value="FNR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF185">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="4 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="13">
        <location>
            <begin position="57"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8106849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="636" mass="73317" checksum="6FB83DBFCC08C3EB" modified="2022-10-12" version="1">MSLFLQTNSKNHSEQSNKFLQSKSEEEFGFVALNNESEDEHTAFYYHKWTNFIVWGVLADFGILANRYGSMSKHRLNLHSIIMGLCVLPTVIAEILMIAIWNPPEFYGNLNLSSIHAPIGFTYLGLMILQSTGGVILKLCIESNNPQKYTKIMSIGHIYLGYSMYFLGKIQCGFGFYEVYSNVQGKGQGNLIMFWIVYGLLFFWRIIFEWLYQNGKLFEYFYSATQTSEKTGSIQDSLFVQYLIQNDQLNIEKEYNNKMWFIFNNSIVDLTGFVHPGGQYIWEKTKGREISRFIYGGQSLEDGNSRAYTHSDKVITLIQRQTIGYLNGNSIENTTQQQINKWTLINQYTISEKISLFGFKNSSKQIESQLTSLHQFGKYYQIKSSVNKKISVRQYTSVVSMAPENVQYRQKLINLIQVLNQIKSEDIEQINQQPRYLNELPLIIKKYESNLGFSQYIHTHKGEEYEIEGPYGPSLGLPNKGRVTIICGGTGILPFLDLLDFQLQSSIYQIVKKKLGQKMAEKLNPFECQFSNGLHITLIIAVADKSELIGQEIFKSLITLQNQLEEQSFKMILKIKEQIEGFTCVNERFDQSFMRQQLGQLSQYDKFYVCGPPVMNQTVPITLINLGVQEKNIHYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5B1</accession>
    <name>A0A8S1Q5B1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1450001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5B1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="170" mass="20292" checksum="ADA8D72219B86B14" modified="2022-10-12" version="1">MVPSAVRAPLVSLFLKSIINQIFIIYLSHYVQQVLWNSDLISSPPFVYIKQQKYQFDANTIYLLIDNIIVIYAIDNYIQFINQDLEVNSFNTISYIYFQRFYIWKKDEIENLVKKNKIVSFVPNLADQLAYVIKQKLINIEMGFKQIYCISKTQPYFLYQSIVFFMINNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7W9</accession>
    <name>A0A8S1Q7W9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1470016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7W9"/>
    <dbReference type="OMA" id="CEMEQIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="358"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8111265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="506" mass="60161" checksum="8C1C802259812BC6" modified="2022-10-12" version="1">MQEILQIVFDLGEMKQETLKVYRDSDPVQLAQDFVHQHNLKNEAVPMIEETIWHHLKQMTINKSQTQDNIFDRLHKEAQIKQQRKQIQFANQQLSQRGNMPSPVLNYNPGDILYQKGIQHKEQMERLQDQRKMEQSQVEYPFKPFISERSQLIAKRPHKQSMPDYLIQMGKDISKHKDQMRTSRYQIETQECSFRPNINQISQKILMEKSRNSQSNIYDRLYNEGMQSKVKRMSNMENTESQSQSQLFNQQSSRKHSKTEIPFLERMQISVFEQRKKKEANQPQQEYDQSTGQKLYHPQICRPPISRNADHLPIGEYLYQMRTVKEDKFHFLIEQDRQEQQNQRAKSLDKSNQIYELKKKKFLYEIFNLLDSDGDGVISAQRIEISAINSEILQILTPLLCEMEQINAQLNQQQFYEAASRLIQTLPIVDRDKLLKGLSNKKKTNQEEYSFQPQLNQNSMRIVSTRQNHSASNHKHQLQQKDEMAECTFKPKLYTPLKIYEFMLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QC06</accession>
    <name>A0A8S1QC06_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">cholesterol 7-desaturase</fullName>
            <ecNumber evidence="9">1.14.19.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T1520016</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>cholesterol + NADH + O2 + H(+) = 7-dehydrocholesterol + NAD(+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:51644"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17759"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.14.19.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:51645"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>cholesterol + NADPH + O2 + H(+) = 7-dehydrocholesterol + NADP(+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:45024"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17759"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.19.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:45025"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Steroid hormone biosynthesis; dafachronic acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the cholesterol 7-desaturase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.21" evidence="9"/>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QC06"/>
    <dbReference type="OMA" id="TYFGPAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170056">
        <property type="term" value="F:cholesterol 7-desaturase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050584">
        <property type="entry name" value="Cholesterol_7-desaturase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045605">
        <property type="entry name" value="KshA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266:SF32">
        <property type="entry name" value="CHOLESTEROL 7-DESATURASE NVD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266">
        <property type="entry name" value="IRON-SULFUR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19298">
        <property type="entry name" value="KshA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="13">
        <location>
            <begin position="57"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8112537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="438" mass="51493" checksum="A5581651AB8259F4" modified="2022-10-12" version="1">MDWARLEIIVPLILIFIYWLYYRKFRFYLIAEKAQPNVLRHKLARGTPPPSYPNGWYRLCRSTELKIGQVEEIKFAGRHIAYYRGTDKVVYAVAAYCPHMGANLGIGGQVKFKSCIECPFHGWAFDGKSGLCVNSEKLDPKIVSTYCYNNIEKMNPNQKGEYLQKVEEGEIKIKTYLVKELRGIVYIWLHCLEAKPWYEPVLEQSDHLQLRGESVNYVNCHIQEIPENGADARHFDYIHSSVNDYIPTWIVSFKWTMKSLPANDPDFYKKMEHSKEKVRKYQKNLFDQYLSNKDLRQYMNVLCLDAYLVFFNKWKFNVIWATGFQMGPATVALYVLSDVFEALYKQAIQPVERFNLKVFHTLYTTSSLPYWASAYILGGELRQVIADVSLWNNKIFTDKVAYNLKGDADPLLLRWRQWYAQFYEGSREFEKSLDALEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QC08</accession>
    <name>A0A8S1QC08_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1530105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QC08"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="34964" checksum="C7AB152C1F32C474" modified="2022-10-12" version="1">MQFNKNNSIFIASCGDLVKVFYFQYGALSFLQQMKHHQLSVTFLSFVRNSQYFMSCCDQQIKFWSINGINNQKFITKVYENERHYSCFAISDKNQLVVAGIENRIQFLYEDNQWKVYQYIENHNDRIYNLSFNESENILISFGGDYKISIMQKFNQFWKIIQIINVDGFGNQATFIGDNTFIFHPWCSKYMFQYILKQKNNQIFCLSNKIQMDGEGNLCNLCFSQYIMQKRLLIKQDMNSILIFQFIENQELQFQQSLIFQSIQIQASMSNDGKYLITWDGSSRNIQVISIEYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QC45</accession>
    <name>A0A8S1QC45_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1550020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QC45"/>
    <dbReference type="OMA" id="QPLCIQS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="24486" checksum="3472EA3D3F4ABDF8" modified="2022-10-12" version="1">MVKSQECSEVLKQTKKYQKIDKEQRKIVLELLLKEKLSLQEVANKLHLKYCTVRTIQKAFEKDGRIGKKETRKKKLKVQSILKISVLNPLTLQIQPLCIQSDNTQMYVDKQPSLIDQLNLTNEQKQLISSQYHKLTQALTSQIKKATLVQQSLLQNLLLSTQMVFKQILDPKPQIQVKQEFDSVSTNSYPFQPIQSYPQFYPLPHTFLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCH7</accession>
    <name>A0A8S1QCH7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1500045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCH7"/>
    <dbReference type="OMA" id="MQGTEIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737:SF6">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VIT" evidence="2">
        <location>
            <begin position="31"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="349"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8112120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="890" mass="103517" checksum="2C00C0E7814C8ED4" modified="2022-10-12" version="1">MITNKLFKPKLESKQFNVSKKVRQTCCFYPQRLIICYDLIRKIPIPLKGVHYFLKVQPGFVIVEIKQTYSTQGYNQPIELEYLFSINNNAAVTKMVVELGDIKVYGIVKEKEEAKQQYEDGIKQGKTMAYSEQDEEFPEIKRVKIGALAPDKQLNITFEYIQPLDVVLNKLWKVEVQPMVDNNYFSLNQQNQRVYNNYFDRLNRYLEKFVQIDQFEYNFKQDITVSIDIGSPITYYKCPTHKILSGNAKNEYAKQEIGEEKLKKLYLMLEDTPSNFIPTKQFTLLFSSDDINLPRSILSHTNNDALYTQKYCATLTFIPKFNQTTLDDAYSQYLDGLNIAENQVINRGNYLFFIDRSGSMSGVRIKRAKQSLILFLKSLPEDCRFNIISFGTVFRSLWSDSKQYSQTTLEEAISHVNSMEANMQGTEIFKPLQDIVYNKQYGKSKTTTLNIFLLTDGEVDAAPIIDLVKRNNQAETRVYTLGIGEGCSQYLIKQLAEVGNGKYQFVSDNEDINAKVIDLLEDSLTPYLQGFNLESNITCINQIVPNPESVVCLKKNQELTIQVLFSVEQENENLQLTLNCFDPQEQKPIQYSVSLNLNNSQENEYFHKLASHKFISYYENGVKYGDNHLNFIKINKDTIDDQDIINTSITHQILSSKTAFVCEVCDLEDQFKQQMKQKVYITMIEQNIRYQKKMEEKIYVPKIQGLAFCPSKCIPPTSSKMCLPQSQFTSLAPVDSSLKKKESQIRFRQEVNDIQKKSKQLSQKITEEKRIQKQIEEDIDIEDELEERQQLQTQVKKIEYEQLVSYSQADGSFLYDGLIEKQINIEGRKNEQGYPQNVWLTLLALLYLDQFCLEYRKSWQLVYQKGIQFLQKNGVSYKQLKETELNKQHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QD35</accession>
    <name>A0A8S1QD35_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1550001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QD35"/>
    <dbReference type="OMA" id="NECQQYC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="573" mass="66477" checksum="5C66025AD5BC78E5" modified="2022-10-12" version="1">MVQLKLLYGNIFQSKSDYLDIQLAGFQIFNCPDFNNLQISCHPTCKECDGPTKDDCLSCYEFSNRRYISEFKVCICEYGTIDQNNECVNYQTLQLNKIEVQQLEKQCQYGFFEIDDDCYQCPSRITIDMITCLECILNPKSWINTLFCQTSYLVHQNGSVAKNFSYENLQYIFTGDELKYCPNCDLNLKAPYDLIEASLIFKNFCTASQSINDGCYFCQDECERCAILPTNELCLDRTPNVSFGNKTCYAPNYLNFYKKCVSCELENCLYCFDYLASDPTKSTLESLENFSLNDEEIKIGCAQCIEGYIFEFKTSTCINQKPIQQNCLRSYINFDDREICTLSNDFSISLEKINCQDLILHCKQCIKTIQTTVKCLICEDGYIVSSITGVCSICPIVNSKQCQEDNLLEPGKWKFQGFIIQFLPPKTRINYDLSRSSSIVIECLQGYRILENECQQYCDEFCSVCETEKITHSSTFYCSQCKRNYYKELIRVQADRQCIECPSLCQVCQKRPTDEINQINPYYIQDAENSIYTQRCIQKVPFDKNIHIDPMLAIAQYCYQDNCNYKLEFSYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QEL6</accession>
    <name>A0A8S1QEL6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1560096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QEL6"/>
    <dbReference type="OMA" id="NDYRDDN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39867">
        <property type="entry name" value="HELICASE ATP-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39867:SF1">
        <property type="entry name" value="HELICASE ATP-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="842" mass="100482" checksum="BB6227717A521D5A" modified="2022-10-12" version="1">MRQIRIESTDKLNKFILSKSNFHQKSISMDKQAQERLSLDVITLNSQLQNEMPSHFKEQFKQVASTILSALKQMDAAPLLVQSYQALQRLMLQLLKNDYRDDNRESKTLREVKEIDKLKVNSLLVQNNKLAIEIKQLSNKLKELEESQDVVKLRDEIQRITQVKDEQINRLIILNDTKEKQVSKNSQLIIQLKSVIQKQDSQIQILENYLESDKKKYSHIENTYDKMVNENRHLNELCKMQEEDLTAHYIRYRHYLREEANSRKKLLMLQDEIDKMKIEKSKRPEVQKVDPEQEQKLMRLAYNFSQDKQYFKIMKELTQLNITKLSDQEQTTIMFLKHVRYDYIYYDSLSKFQYPRPSFLLFYESNMKNLEQDYRNIEFHQISILYFATIRSILDSQYNEFLYRDNQYTPIKFADFVYGWISNFIINPKTRQIEIIKDRSFEHLEPIRNDFILDCLSPKLAKLHEVIIFNELLFELSSPDELYYYLYSRFLIFKGSLSNQQRYSFEAVIYVQLNWVQQILEMLLWKYDQSMYNQINQIIIESSVTKGKGTIVDGHYVLRILLELYRTERRNRLAMLKEVFSACISVQSSGKSGVTYKNFKKIFQSNFPDVPDQEICSLFRESFMCGDGVVTAESFFTAASESCFFVKQMLLKSLIKTPIVELNEISYKESNLPFLDFYQHYKQLPHHLVQKCCLQFGLHSVIIELEELHKLIKNYFQDQYNQYSLFSTVTKLIHIYNTVIDLHMRQQNTFNKDWDQSEIESLKSLNNQLSQLFSPLIDYDQQKKLQNIAKQRKLRPLQLFVKNRVQRFYQMIANIINNNNINVADPQVAKRRFTKQSSIKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QET1</accession>
    <name>A0A8S1QET1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1590107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000164">
        <property type="protein sequence ID" value="CAD8114419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QET1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="10962" checksum="EF0AA7E30984221A" modified="2022-10-12" version="1">MNGVSCIIVQGKIVSIQLLKIQLLVRIIFMLMMIVMINLETRQKQKQNLFSNDYHSSLEIDILAQIKLMINSSGNIYVKSKSKKLDVVKELFCFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QEX1</accession>
    <name>A0A8S1QEX1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein bL12 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1600030</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QEX1"/>
    <dbReference type="OMA" id="PPNNPNW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00387">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L7/L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_bL12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_bL12_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL12M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL12 C-terminal" evidence="5">
        <location>
            <begin position="174"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="153"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8114479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="241" mass="27210" checksum="C9285F442B10C068" modified="2022-10-12" version="1">MLTKSIRLFRQFRFCSAMEDITKKQAEWSAIAKQKTQQQQDYLEQTLSQDQKLRVKTIADACCELDLFELQCLMSLLNENHYKAQGIGLFEIDSNWPVIKQNEVPTWPPKEKEQIEAYMKDLFGENVPDFKAIFGGVGSIANQAPAATQSEAPKQEEEKKADAAPKVEAEKKNYDIELNAIDAAQKIKIIKEVRQLLNLGLKEAKDLVEKLPANLGKQVPKEKANELKEKLTAAGCTISLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QHI1</accession>
    <name>A0A8S1QHI1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1610087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QHI1"/>
    <dbReference type="OMA" id="ESKRCNY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="105" mass="12236" checksum="22CCB0A2B4EAE9F4" modified="2022-10-12" version="1">MEVIDNKSRSYCKVSKQKQEQLLNLVYKDEFRINQAADFLNINYATAKNIIRRFKKIHIFRKCGNISESKRCHYKQIVEQKEQKNGINTKGGLIGDCLTGQVQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QIH0</accession>
    <name>A0A8S1QIH0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1610095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QIH0"/>
    <dbReference type="OMA" id="SHIQCNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="49"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="587" mass="70080" checksum="3FE8D22E575CBC43" modified="2022-10-12" version="1">MLPKRQYSFQDQYPQQKTSPLQLKRVHSVQLQDESVTQLIFTHQVIKNIEIVSISKEKDNFSLHKKHIVHYVIKITTDYFDYQIQRRYKQFLQLYEQLKPKFEFCEFPQKKLFKSNKSEVVQERRKLLQKFLQTILSSLDQLKPIEFLQFIEIQQQFMRRGFDNIKLHGLSSDQFNLDSLLEPEFQKHQPQNDIEKAVICYLHKLNSRKEDRVKIILQLENYLYGKLQYLSYNIIQYLFIGDKDKEIYGLTQMVLNDSDSHIQCNCVLSFLSKLLEFEYNPFAEVTLKAFCELNMKQIKQMKLQNHIKGNQQNKCYKNALLLLYRYLEGNPLLDQKILKFLIEDEEVLGIYQNFKSQKQTLDSTKTLDSSDESITPATTQETIRKFSSKDMILIEDDNTSEFLIKILNLNYDWKLVKQYRNCSLLHLQGQIIKNSYILKCNSIEKVYKLFTEQKQWRNSFKKFEIFETIDDNNFIALETSIWSIKSSFQTFVFFSKFSIKEKSEIKISFTVEFVKDCSKEYMSYLKKNELLCKKVSYVEISKIDEERIQVVTITNLIDQEQKNHVLPSLLNEDNSFEVQIDKLYQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKY9</accession>
    <name>A0A8S1QKY9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1630085</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000170">
        <property type="protein sequence ID" value="CAD8115300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKY9"/>
    <dbReference type="OMA" id="CCKESET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097039">
        <property type="term" value="P:protein linear polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000060">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF13">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="194"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="198"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8115300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="407" mass="47795" checksum="904899EFAF46F90B" modified="2022-10-12" version="1">MNIQNISADQELVQFINSPQNIMENTKRLYELIEVLCQKKSNLQIEYDGSNYCCYCQEFLIENNFKILPCGHNVHFECYKNYLIDQPFYIESIETYSCCKESETCSQVIITEQLSKLILGDQQFNNLRNQFELDQDDNNDLSDLEEYPFKVEQDPNINEENEKQIQDSIENDQKIAQSIQQQEELQKNQNLKKINFDCLICFESYDVESSAITLECDHRFCKQCIQEQIYSQMDIGKFKENDLVCPQCHKSIDFHIIQYCAPDCSQKINDLRVKNLDNNASNERLVRCPGKGCPEQYYISFYLEFPTCITCKLQFCANGCDKVHQGMTCDQYQKKTRTKQEKGLVSCPKCKIQIFKDGGCNHMTCKCKYEFCFVCSKPYKPKRECNCPQNTTIDRFYDRLKDFFGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QLQ0</accession>
    <name>A0A8S1QLQ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1650031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QLQ0"/>
    <dbReference type="OMA" id="MLDPMRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8115550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="180" mass="21084" checksum="849F68F37E41B19A" modified="2022-10-12" version="1">MLDQMRMATVRRQSELIKQNNQLIEQQPRGRQQCVKPHQILTEIVNQCIQRSQHSIDALQQIRARSKDRRSSYKKTTRQSTISSYKGQTSPSFRQDDQSVTSSIQESLQSQQPVRLFSFIENEPSFRAKDMPLQINKASLQAKKKPIISLKENQKKLTNIDQLFQEALHKSQQITEKFYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QWK0</accession>
    <name>A0A8S1QWK0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T3090001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000318">
        <property type="protein sequence ID" value="CAD8119345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="LEIVCQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8119345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="169" mass="20042" checksum="237930D93A10BA37" modified="2022-10-12" version="1" fragment="single">MNILTSQIQQPKPEILEMVCQIHKREIVALDLDTSEKGQLQYMCSNCLVEKINNNKISTIDQTKERIQSFKTQKQENKTKQIQQRLDYFKKILDQIMEYKCNVENTLEKIYSQIKAQIFTIQKEKESLLENCQILHNFQEDVKSLSEFISTDSQDNQMEFQQDSQFIXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP98</accession>
    <name>A0A8S1JP98_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0040443</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JP98"/>
    <dbReference type="OMA" id="GREMFYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681:SF0">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681">
        <property type="entry name" value="ZINC FINGER-CONTAINING PROTEIN P48ZNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="167"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="167"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="261"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="51"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="330"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8043219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="349" mass="41478" checksum="6D85526C9A9921A7" modified="2022-10-12" version="1">MSKKTEKKKQEKIIEDRTFGLKNKNKSKQVQNFCKGVAQQVKYSGVSLSKLQSEEYERKKIEKQLEEEERLMQSLYKTVEKVKEEESEEEVDPKSILCEYYKQGLCQKGKKCMYSHDMSLEQKTAILDLYTDQRVQLGDEWDTCQNWDEKTLKDIVEANEKTYKSQIPSAKVCDYFLDALEKGKYGWRWVCPNGMTCHYKHCLPQGYVFRKKEEPKQRQEGDLEEEIDEQISQLQKGGTKITKEVFEKWKQDRAERKKLEAEKQKLEEQKKKGQKQTGGNSQMTGRALFVFDPSLFVDDEEAEKEYEREEIVDENDEEDEDQNKQKLYEGDENQQQDEEFDQQFKQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPH4</accession>
    <name>A0A8S1JPH4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0050291</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPH4"/>
    <dbReference type="OMA" id="QYLFKMG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20908">
        <property type="entry name" value="SUF4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23215:SF0">
        <property type="entry name" value="BUB3-INTERACTING AND GLEBS MOTIF-CONTAINING PROTEIN ZNF207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23215">
        <property type="entry name" value="ZINC FINGER PROTEIN 207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8043637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="188" mass="22783" checksum="18D92E20A5A09889" modified="2022-10-12" version="1">MYQEYNLNKNNYQYLFKMGRKNRDLSYLKPFCFYCDKTFKNEVYLHQHQKAKHFTCQRCFKKFSSCDSLKNHVESAHHEVLTKIPNATGDRSDIKNKIFGMQGVPRVEIEKRIRKGAEEYWTKILSAQYQQRRKENKQRIKQNKLVAKQENLQEIRQENEQEEINQYEYNPEAKPISFDLDFDQLPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQ83</accession>
    <name>A0A8S1JQ83_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0080128</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQ83"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46858:SF5">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE APD1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46858">
        <property type="entry name" value="OS05G0521000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035725996" description="RING-type domain-containing protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="199"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8044564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="282" mass="32367" checksum="3D126F5C5D8D3CAF" modified="2022-10-12" version="1">MISNTSILLLIPISILNFHNLGNYGNIIETTRIILIINQVIFSYFTARFKPELVFSLQTYLSLNDSVNPLNTLPTQQGEAQLQQFNKQDFVESRVSEPHMQIPKVVKRISKTYFGFDDLPSDKKSETQLSGKKPTHHKALSSQIQRCSDDQNEKIKLSSLINIKLSERQKSIIDIENNSQEIEQVKVPRSVSLSSINSCCICFDNEPNALFMQCGHGGVCYNCAIDLWKNKEECYLCRNKIDRVLQIKMIQQQKNIYQVVAATELNQKLKLEQKILIQNQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRS1</accession>
    <name>A0A8S1JRS1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRS1"/>
    <dbReference type="OMA" id="FCTYFRS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="257" mass="30531" checksum="2FE49AE7D0A1082E" modified="2022-10-12" version="1">MQYIQQLDLSRYIPGGEALKTPCNQNKPDKVKLWEQGKLRCVSYCFQFTGRMAREQRFNMFCTYFRSKLHVLHSKLGSEMHCRNFIKEMLDTTISIFLLIVRYECGDDLYQEMRKYFKILMDAVDHLATHDSVEQVTEIITPEDFHKIIFYLKLLNLMRNIKQRKKFLEHYPHAAIKAIQQCYLYNVIINGPVKYIKYRFAVANQDYAKYGLQFNIQSCNKLVSKQKAAEICWQTLYDLNIAAVIKEGIWDDLKLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JS29</accession>
    <name>A0A8S1JS29_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090426</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JS29"/>
    <dbReference type="OMA" id="IFCEIKL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="86" mass="10359" checksum="766D70D07BE37DE1" modified="2022-10-12" version="1">MQEDLNSSLDSLELELYHKPNQKIKSILTYLRQQKIQFCTDPKKKEEDYYSKNDKESDNKDFGLFRNKFFKRKPEPLPILCEIKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTI6</accession>
    <name>A0A8S1JTI6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0050209</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTI6"/>
    <dbReference type="OMA" id="FTAMPPG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF12">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="601"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="630"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="662"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="715"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="12">
        <location>
            <begin position="24"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="12">
        <location>
            <begin position="811"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="292"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="295"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="309"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="326"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8043556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1000" mass="115994" checksum="8CEEA99EF10D203B" modified="2022-10-12" version="1">MPRFIQERMNKGQIQIQQDQGDVTILFCDIYQFDKVIKYEQENILNFLDTLYRAFDQLCQTYDLQKIETVGKTYMAAGGLKDYDAVINQKNANSTTRALETAIAMMDTVKTMKYGDNQDVKLKIGIHYGRVIAGVIGVHKPQFSLIGDTVNTTSRVCSTSEAGFITLSESAYNNIKDTTKYQFDQKAVAAKGKGTLQTFRLKLQQKERESARLISSKLIKDHQESNNKESRVQIMPLKNNENKKSPAKNLLKRASVMNPMGMGNDSNKSPILVLRQLQQQLQNKKSLQVMIKKKQSIDHENKEQEIKRQNSQKSVSSSNSDNENQFQVQQQPKVVNSNSKMRSSILYLQRPIRRGNEDGIIQHYPSTLQQFSMTQFQPVSDVRILPSSNQISSSQNQQSSSIQPQLSSLQQNSQNQLVQILEHPSKESIKQVRIVEEAQNQQNNQQIPRGQIKRKGTIIMADLVTKKKMMKSNTKIILPEDKERPLKYGQKENENHFEGNGNKQIDNHGFFEAQETLIKKDNDFIENFDEIEINKKQSISKLQQKLDLIKLKNIKKFKLDFDKDFDYELNNSQYDNKDHVLYYQIYEEYNLQELIQFRKTFILLLILMICKSLLYFLLDELNPTNKIELIIVILCQLIFSVLIIVAFYRFQDLQDFKKAKTFSLIYFIIISSLNILIIYFVDEQQFEIILQSCQITAIYINLFHQQLFILKDKQYLQIIYFILLILLTVYKKYILEIVFFIISTDGLTYYYEHQVNEILVKNYRVSQKLQTQISKYENMLQYLMPPHALKRLLQPESDKTETFIDVLDNATVLFADIAGFTKYSSSVQPETVVEMLRNLFQSFDLYCQMAQIYKLFTIGDCYVCMGVLDYTKRDPAEEAQKVLAFGLKMIQIINDVKQDPQYQHLNMRIGVHTGRVLGGVVGTDVVRYDIYGEDVTIANLMESSGTEGKMLISDYTKNLVESEYDDFKFEYAKDVYIQSKDITVPTFFVSLNDADYSEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JX86</accession>
    <name>A0A8S1JX86_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0040043</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JX86"/>
    <dbReference type="OMA" id="YTKRQTE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051965">
        <property type="entry name" value="ChromReg_NeuronalGeneExpr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46040">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46040:SF3">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="54"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="3">
        <location>
            <begin position="54"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="132"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8042828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="167" mass="19924" checksum="041D512CB37C6AA9" modified="2022-10-12" version="1">MQDKKEQSKFENFLIRFKALVESYQTITPEPEEQPKQQVTKQKKQKRQKDPLAPKMPKSAFIFYFQDKREKFQQQYPNLQFQEITKLIANEWKDLPKEIQQSYHDKAFNDRSRYSQESQLYCTQIGKKFNGKSLTKTNKQSAKNEKIQQKVDQDEENDSFGADIGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXX7</accession>
    <name>A0A8S1JXX7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040268</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXX7"/>
    <dbReference type="OMA" id="LQCKKEA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="228" mass="26326" checksum="8D8D2B66D12B4B48" modified="2022-10-12" version="1">MTKRKPQFDIGKDEDDEEVLPVVGVINTAEEIQERKILKIKGPKQIENNQNTKIVITAPPPSFEQAGSDQKLSTPPKKFQPTNIFNSQQEKLFFNTQPLNSLQLPQQQKNNIGLVSPIIEFKSIRKSDKSITSPNQETNKKGEIFQNKLKKIKIGEKEHENKDIILIVQKVGENHKIIIEPDLFEGLVEKEKTKKENNIVKLECKKETDIHELELEFLNEKDYEQFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K159</accession>
    <name>A0A8S1K159_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K159"/>
    <dbReference type="OMA" id="VEWCELA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="250" mass="29207" checksum="660F3E07CA2599F7" modified="2022-10-12" version="1">MFYLFILPIYAIDDIQVRRSIICGCKHLSPSECIEVEWCELASQFDKCPMDADCITYQTELECEEANASQCVWLDNYCLNKCELLEFNTCQNKQIDSNCSQFNTSTTCETYSWCEFNTTTQVCQQILQVSCNDILNSIECEEYGCQWDDVKFCQNKDSDCSQFETIEDCMTQFQENRQCIWIEGKQQVSNIKSSCFQSKECDKYTYDNNELDICESISNCKVDENQCQSCVQDMFTFESILQLISIFYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K215</accession>
    <name>A0A8S1K215_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K215"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8048729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="81" mass="9942" checksum="A5227AB2646C428E" modified="2022-10-12" version="1">MLLMPLQKIMKMDYIIIQRLQILKIRQSIPYLWHLNFLDSLNIYYQDNLETDNYLIELFFQIAGKMQICFLVQQQQNQKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KD34</accession>
    <name>A0A8S1KD34_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200188</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KD34"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="370" mass="44348" checksum="BEF1BFD9C73820E0" modified="2022-10-12" version="1">MPRYPIQQFNQWRIYITKQNISMINSFKQSYLMQNIGWMVETMKTLQFTIKQFNQKFLRNEGIIQNEMNQSKLEIEHERDRRIYKDLSKSIDTIISQILGIQEKELREQIINALLSPNNENQLQWSLKLQEEGKYNKATLIKKFIIIYHIKKRKIYLQGLQKNDPLVLYEKYQQFSLLKYKQKTQTFGNEQNTNTRISTEQSSLRKQQNVQIQSQKKYSYNANLNQQSTNKKQQVEYGSDSQEEQLFCKKCKKGLYSFFLNLQNTFKKCQQGKINDQQEKKGRFQKGGKYNKFQCSLRFHTFQFLQFFSGFSMIGRNTIKNIDQPFSIWEFFRCKSQILFLSQYLTQLSVQVNFSSVSYQISNFNISLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KF14</accession>
    <name>A0A8S1KF14_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210347</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KF14"/>
    <dbReference type="OMA" id="THYEVPK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="191" mass="22958" checksum="932771F572BC3D9A" modified="2022-10-12" version="1">MYKIYFTKRRKVFQYSETTTESQLNQLLQIIIFNGQSTNYDVSKIIEETEENEGISIPKGNRFQNWNDIFSDFLSESKQTQLKRNTETPIDSAYLYEVPLQFDSALSISPSIRKFVIRKIKLKNLINQFHQLSRREKDQKINVTYHRLAVIDEGNHEIAFLYLLDEKNKVKLKNYRHQTYFIYQRSNRRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KF64</accession>
    <name>A0A8S1KF64_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KF64"/>
    <dbReference type="OMA" id="HDKRMLV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006353">
        <property type="entry name" value="HAD-SF_hydro_IIA_CECR5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01456">
        <property type="entry name" value="CECR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF93">
        <property type="entry name" value="FI11325P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="327" mass="37722" checksum="7DB02D8FD3F9000B" modified="2022-10-12" version="1">MKKLIPAIVSDIDGVLVRGKSIIPNSDIVIKQLLNCHYTNGERHNIKIPFYLLTNGGGCTELDKANQLNKIMGSNFKRHHIFLNYTPLRPIMNEYQNKLILICGAGKLTEIAKDCDLKYYYTIDEYSAIYDQVTFKQYDAEIIRQYEADIKQRHMEQMKNKQIEAVFIVFDPIKWEESIQTISKLVKENKDIPIYVVNNDVTYADQFKLPRLAFGTFTNALISILKKEYNINPNIIYYGKPSLNTYKYVQTYIHEKHENIGNIYMIGDNPTSDIRGANLIGWPSFLVRTGVFRGGENDPKDPGKYVVKDLMDAYNKILQLEGLKVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KFF1</accession>
    <name>A0A8S1KFF1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KFF1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="60" mass="6913" checksum="C6FB32EFBC019D15" modified="2022-10-12" version="1">MIKTYDDKIQSQQFDQYGIFINEENTNQLPEGFKGNVICMKLINHTESGNSNVLKIQNYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRH0</accession>
    <name>A0A8S1KRH0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0200388</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRH0"/>
    <dbReference type="OMA" id="XIDIKLE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01861">
        <property type="entry name" value="Rab6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000823">
        <property type="entry name" value="Small GTPase RAB, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="174"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="174"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="208"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8053554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="220" mass="25068" checksum="79F58FA535F8B30A" modified="2022-10-12" version="1">MAQNTTSGPMQKFKIVFLGNQSVGKTSIINRFIFDNFTGNEQPTVGIDFISKTLQVDNKSVRLQLWDTAGQERFRSLIPSYIRDSQAAIICYDITNEKSFQDLQKWIDDVKDERGDEVLIYILGNKTDLEQERQIQTDVAEAKAKELGAQFQEVSAKSAHNVAEFFKKLSYDLQGNPNEQQTQQQPQQQKVPQQQQQQNQTQQLKINDVTEDKKQGGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KS38</accession>
    <name>A0A8S1KS38_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0210081</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KS38"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8053804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="212" mass="24150" checksum="E471FC8B710E00DD" modified="2022-10-12" version="1">MSYQFGGSQLQTQSVPNAKYKTMLCRHYLATKQCAIGAKCQFAHGTQEQRQMNDPLPASALSAMASGIEQPVNKPQTSVFQIPCKYHAQNYCKNGSNCQYMHDPDSVQQQNTINQQQMQVISSPLPQTKPEDPMQIVFQHILIEMQQIFPQDEIIQKLKIAFDQTKIGNLINASECIKSIIHAPERTHEELLKYTNLYNQAIQYFNQISQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTF4</accession>
    <name>A0A8S1KTF4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTF4"/>
    <dbReference type="OMA" id="SYRQEND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="42"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="213" mass="25765" checksum="43360A2B9BB09226" modified="2022-10-12" version="1">MLSESNIQIINKQLCQENFELKLKLGNLKLELERQVILSKEGQEWKDKYEKLYKQNQSLDQENKQLQDEITKLNNQLKNLEYSVQQQLEKTRDLNDYEQIKNNSISVQKHNLELQRELEQYRQKQLFNLKSKIINDNTKNYYQIILEWDNNIKNLQLTYKQENDSLIFDGYGEREDKKIVYQQIHHLEKKTLKQNMKPLISLKQSQMTLIFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KV74</accession>
    <name>A0A8S1KV74_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0270147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KV74"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="1">
        <location>
            <begin position="49"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="172" mass="20333" checksum="7A1559385175C519" modified="2022-10-12" version="1">MKQQQEEELIIKVKYNQHLICILQHGHKNQQKELKNVLKVVQNIQFIYKAQQAFLIYKQRVHTDKWNIDPEFILNEWMQSKQSAQQLLQSMLFQIGCGRSMNSNLGEYVEYIVCYFDYPNKLEPYRPSSSLNVANSCRLGRSSSYIALCASPYQKVQGIMQIRQRHRESQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXK1</accession>
    <name>A0A8S1KXK1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0260023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXK1"/>
    <dbReference type="OMA" id="ETECANT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="399" mass="45551" checksum="151AB15C8D1DD42C" modified="2022-10-12" version="1">MSEDIKTRAINYLNCTLGDMHEGEQLNFVCLDTTCKEMGLICPVCRSQKHAKHKVIPLKIFLADISNNVNGKQNQNSIDQLLTQIDQVRTKLLSSLKETVQKMVLQIKLLEDQINLSHKNTKQRLLEQNQTQMNFPQIFQQILNSQYKNVDQLKQDVRKIIENVSQQQAGKIEIDFKPQRLFDQYQKASQEAIAQFNHLLTQFKSSTSKISKPIEKFTLPILAQNSTNFTFSSVIKSPSIVITEQKNAHQTANQNSENRFILIEPQIVESCKIAIKISNLTNFIGIGIAYKNVLLGKNMKFDHQNLGHGSYMISSNAHTWSHSKKEENMVQKSFTFTTGDIIIVEVNLENKTLKFTCKNKPIDTQAITLTFDNPDNDDIHFCINMCSSGEKVEILDDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZ96</accession>
    <name>A0A8S1KZ96_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0260338</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8058016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZ96"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="6"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8058016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="70" mass="7580" checksum="70CAF283AB8789A0" modified="2022-10-12" version="1">MNQNKSNCHSDYGKSVCFSPDGNTLVSGSNDGSFLLWDVQKGQQKAKLDGHNVTVSSVCFFPNGNISLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6K6</accession>
    <name>A0A8S1L6K6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Radial spoke protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0310161</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flagellar radial spoke RSP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6K6"/>
    <dbReference type="OMA" id="PWIMGKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009290">
        <property type="entry name" value="Radial_spoke_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21648">
        <property type="entry name" value="FLAGELLAR RADIAL SPOKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21648:SF0">
        <property type="entry name" value="RADIAL SPOKE HEAD PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06098">
        <property type="entry name" value="Radial_spoke_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="373"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8061143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="47267" checksum="53927E46EC5B7CF5" modified="2022-10-12" version="1">MAQAQALAGENYEFKAAPEVVKPKPKYRQQPLQSSEDQKNSSLLGCNIMYDKRVVRGNTYASVQTLGDKKLMTLKSFKKPQQKATQQPQTTYLHPSTPRPFQNRVNIDIQTDEYLEILRDKPPEQEMDAQTDYYIDKPPDRLFVPKKNGIDKETQIWEGDLFDFDQEVEPILQVLMNKILEQSRMEVLEEEEIKLMKEQQKQHERQKTSVLSEMQRLEAVQQRLDQEDARRKLQYETFLKIQKQSHQKIVCRNLSKSLINPLKQNIIKKLQDLGVFRDPLETALLYEYIPWVYDNVLSQLVDENDNYTNFNNMLNNIEENITIEHSNSLKRRQQHIDDKYEEIARKKREKIEKKRMEEERRLKEIEDQRLAEEEAVRQAEQEKLDQQQENQQQQLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L8C6</accession>
    <name>A0A8S1L8C6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphatidylcholine transfer protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">START domain-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">StAR-related lipid transfer protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0330175</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with ACOT13/THEM2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L8C6"/>
    <dbReference type="OMA" id="QDIQCIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00177">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000017">
        <property type="entry name" value="Phosphatidylcholine transfer protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF14">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="13">
        <location>
            <begin position="466"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8062515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="669" mass="78647" checksum="D9740446E3E78B1A" modified="2022-10-12" version="1">MQVQQMLNIQKFQKMWNYCSQFYEFGILHPTLNILLGIEFSALASTPSKFIQLLLSKHFYLSQNYLTPKQTKEFEDVLSKCDQFDQRNIGTVIGMILLGAIGFFVFGFALVGGIGGGVLGVVGGHQVGKRIKKTLQNKQDIKEIDIYDIRIRTILKWAQLQKKKYCYNINLQRFVIDKILTEIKIALHFKYYSTINQTQLKKLLKKTFIFLNQDLYLCTIELSLLLLEEQLKILKYFSLQDQNISLNEEEIKLVNQQIHYRNQLEISVLKGIDNIIKPVVTLFQNQLPEHKLPLIIKKSRQFIYSNEIVQLSKQFPDPQMSIYYIKMLSSQLNQSVNTTSLKLYVQQQNFRNLENVGQDIQCIMKIQNIQKEDEIQINIEQLQLEVQQSFNDIIQNNEQQVESQQIILQKSQDIKLKLHSHPNKIYFSKIDPKCNSNSTPYQSYEEIIHNQEIQNQLSKYPQHYCQLSIEKFELFLKLIHEPTDKWDLAINTKDAQIYKTLKEGSENVFIKGFGIIKNTTLDLTLKVIHDIKLRKEWDQLLRDFQILKTESQDIDILAYYVQPPISLITPREWVQRRILRYDFPEKGQITLIFYSIDYPQHPINRNRIRAHTEISSMIFEVYEQNNVKISICSNIDIKGYIPKMIVNKASTNGPVDWFKSLQRACNKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L976</accession>
    <name>A0A8S1L976_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0350146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L976"/>
    <dbReference type="OMA" id="KFKIIEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="53" mass="6290" checksum="93AAF50056B34B8B" modified="2022-10-12" version="1">MDNTETTVRNGNSEIKFKIIEWMGNNQDATNLLKIKKELAKRILREQKKPQQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LES6</accession>
    <name>A0A8S1LES6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4'-phosphopantetheinyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0390196</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LES6"/>
    <dbReference type="OMA" id="KNFGNDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00101">
        <property type="entry name" value="AcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002582">
        <property type="entry name" value="ACPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00516">
        <property type="entry name" value="acpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4'-phosphopantetheinyl transferase" evidence="5">
        <location>
            <begin position="6"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8066608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="129" mass="15084" checksum="E7747B13F744B439" modified="2022-10-12" version="1">MKTIKGIGVDIVNNQRMLNIIKAQYAERFLVKVLHPKEIEQFKSKSTIQLQQQYLASRWAVKEALVKASSFKELVFPEVEICRKQEKPNIVLHNKNLEYFKKNFGEQFEIQVSISHEDSYSIGFIILLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LF43</accession>
    <name>A0A8S1LF43_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LF43"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="59" mass="7136" checksum="4C5382179BDAD891" modified="2022-10-12" version="1">MGPYYPHIQQPQLHPQINQQQSTPQQLQQNIIQNRPNIKEEELDDNKQFKGNWLQQKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LH19</accession>
    <name>A0A8S1LH19_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0380084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LH19"/>
    <dbReference type="OMA" id="FAKINIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000121">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="1">
        <location>
            <begin position="246"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="447" mass="52830" checksum="14A6D9B38EC45E67" modified="2022-10-12" version="1">MQQNEMIYLQQKTGDKLKNQKLEQMISDNPQNTQFTQQFLQQQLDKNSIQKQGTLLTQNELSQAIKKFHQYISKNQHINSIQTQQQSQETQNLQDNPKSKTINFDTNFFNVQPKHSYKVAQDLSTKSDNKTHSQHFRQTSNIIRNNGIVNRQIVNSKPITPKCGTFQFTQYSYIQQEQTTRNEEEIYYIQKVKQAILLQDQNDYLNKVYRNHFKQLCQELSIGIKLKPANYIDINKKGLKLSQQNKYKDSITLVFDLDETLIHCNQRDQKSYDVILTINLNKTQQVFAKINIRPNAVEILKKLSKDFELIVFTASDQIYAKSIIDYLDPNNNIFAHRLYRESCILTSGGILVKDLRILNRNLERVALIDNSAFNFSQQIDNGIPIIPFFDNKSDDELKKLYKYLSGMKEWYDVREYNRKHLQLYRYTQNIQNLKRLQQQIDIIEDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIK4</accession>
    <name>A0A8S1LIK4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0410023</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIK4"/>
    <dbReference type="OMA" id="QTENFIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF150">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE STK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="126"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8067510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="482" mass="56633" checksum="C59189C6870B0E92" modified="2022-10-12" version="1">MKKTITLPSDYSKSSFFNDGKELFCNSPQFQMEFTLFGTFLMKSNNRQYNVNIFLHEFIMKIIEEKGERHINLENVCVKKALHPKTQQLGLRISKNGQFIELFGEIIELWKQIKKFVIQTDFSLKYKLGQKLGERFSSSVFKALKIINGQKVAVKIIEKSLVQRNNASERQQLINELTVLRQINHDNLLKLHETFENDEKIYLVLDLLEGGHLKNSIFRFRYSEQETIILMQTLFKSLNYMHERNIYHQDIRFDNILLKDQQDLTSACIINFGKSEIIQQSLVSTQNLNFYDQNSLIQFIRKKDILSLCIVIYSIFSKKLYQETQILDELLMKNYESMSDIEYLDLNPPLLQFFEIFFTSKGKTQMDTIKCSDILNLDIFNLLLIRRPSKFQGIPQQINHNFKRTSRFAPKYSEKEQQIISNFQKILKLPTISDSSESKISQNNSQSPQTENFISYRLLQDEKASSIKPRHSVLKKCLSPNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIL9</accession>
    <name>A0A8S1LIL9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0410307</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8068068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIL9"/>
    <dbReference type="OMA" id="VNHLHFF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF31">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8068068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="338" mass="39376" checksum="C65754D98EADFF19" modified="2022-10-12" version="1">MFNNIIQSKYYPIIGSSLIILFYLHYSLPLLTLTYFIIAYYVHRYQRGPIRIYNQVNLEGKIVIITGASAGIGKETAKRLAWQNATIIFACRDELKTMKVINEIKQETGNQKLHYMNLQLDDYDNVKEFVKNFKIKFGVLDILINNAGIAQWWHKYNKNNHELVFATNYLGHFLLTRLLLDSMSKESRIIIVSSVMHELIRSKLNFNDVLNGKYLENYNNTKYCGVLLSLKLSQILKNTKVVALHPGVIKTELLDKAGNTILLQIIQKLLFWFFRPMTLSVEEGAETTLYCALMPYRELLNGGYYEKNKLSKCSKTIEKLNQIEELWEFSNQILKEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LME2</accession>
    <name>A0A8S1LME2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphagen kinase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0410004</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LME2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="6"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8067472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="61" mass="6681" checksum="9D69A5F79217E328" modified="2022-10-12" version="1">MGTGKRQSILGKFLNITKQGTDEKKLKEIAKQFGLQARGCGCEHSEMDKEVTADISPSARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LN50</accession>
    <name>A0A8S1LN50_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rab-family small GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0440148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LN50"/>
    <dbReference type="OMA" id="VKEFWIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001329">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23516" checksum="60F9B0F4EB070422" modified="2022-10-12" version="1">MIKIDLIVIGNLGSGKTSLIQRFIDQDAPFQHNYLPTLGLNLVKKEFVQQGQQIKLCIWDTAGQDKYFSLTKSYFQRADGVIMVFDIADKDSFNRISDYWIKQVQECSKANAQSILVGNKIDLCEKRQVKFLEAQEFSQKYKLPYFEVSAKTGDGVQEAFNFLSRKCTECIETEVNEVQHLQIDSENKSKGFQGCVNTIMSIWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LT35</accession>
    <name>A0A8S1LT35_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0430300</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LT35"/>
    <dbReference type="OMA" id="APIRRWC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000586">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8069305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="23429" checksum="028EADC11E008EE4" modified="2022-10-12" version="1">MEEQLYKIVIVGNSAVGKSSLLIRFCDDQFRDMYLSTIGVDFRFKSLRVNGQGVKLQIWDTAGQERFRNITNSYYKGAQGIVVVYDITNLKSFEDIRKYWINELNHYADENVLLMLLGNKSDLATDESRQVTTTMAEELAKEFNLKFFEVSAKTSDHVEAAFQAFTQQIQETGIAGKIRKPQPQQYLQTPMPQEDKPKKQDSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUQ0</accession>
    <name>A0A8S1LUQ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0480028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUQ0"/>
    <dbReference type="OMA" id="HKENMNP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="119"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="282"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="467"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="253"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8071740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="596" mass="70696" checksum="E92CBA75E3EE02A2" modified="2022-10-12" version="1">MSMQLTQKPATMYQVRQSSVTPPQKQHCNPNYMSCQKMRMSIIDQLSPIKDLPVNQSQRSSFYQVRGKSGSTATLMPNKTQPNRLLDSTSFNTTLNYTQESNFDKKSTTIPKGMKINNFEILKDQLRFAICQLEDLKKSFDRVVQSQFDQVIQVIKSSAQLSNQLVEENNQIRNHLNTLQSNSEYDKLKLIMEQLQQKITVLVNDNSKLNHQINYQQEENKKLIQQISESDRKYQDLLLSQQNRLHKENMNPNMNHNIHINTHPNSSNTKSNYSQRELSYKLEQAEIKLLTLQISNDNLRQQLQHTQLDHQTFEQLKETITSLEIKSQSFLNQKDSLQQENDLLNQKISSLEKQLKDQSQKTEYIVYINQLENDIKQIRQEQNNNHQTITSLNNTINQLKEQISILKIQKDQSENTYIQQVQSQQNELQQASSRYQKLWQEISEMRERLEQRQIQEDSHNMISNAEKALFETQIQNLNDKIKDYEQILLHYESKSRQLEQQVSQNQLEMDNYRRQLEHMNNYNNNSLEIQELQWKLNQTNQIIQRKDKENQVKIDELQSIKQALDVQVKKNQNLEMRILHFMEQEIKNNTNLIRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LVT1</accession>
    <name>A0A8S1LVT1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phytanoyl-CoA dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0500076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PhyH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LVT1"/>
    <dbReference type="OMA" id="FKFCQNP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048244">
        <property type="term" value="F:phytanoyl-CoA dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047128">
        <property type="entry name" value="PhyH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21308">
        <property type="entry name" value="PHYTANOYL-COA ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21308:SF1">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="35967" checksum="FBA6A2303280286C" modified="2022-10-12" version="1">MSQRLELLQKQIPSFQYTLDGGILTKEERQFYEENGFIVVRQIFTPEEVKEWTQRFREYADGQLERKYGMQVVRDISLVKKGGGKQWGEEAITKIQDWQEDEVLFKFCQNPKVIQYLKSFCGPDIKSVHTMFINKPPNMGKTSRHPIHQDQVYFPFGPADRIAAGWAALEDANRENGCLVVYPGTHKVGPVEHCYPDWKEGVNKAYWGVKDMPPENAPKIHLEMKAGDIVFFHPYLFHGSGENRTTNFRKSICCHFASANCQYHDIKGTFHENLAKDIIAYASKKFGHVNYLDIWRFKCRLVQGNPDPLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LW18</accession>
    <name>A0A8S1LW18_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0440245</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LW18"/>
    <dbReference type="OMA" id="KWRIGQA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="3"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="400"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8069801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="448" mass="52826" checksum="2381CA50031939DD" modified="2022-10-12" version="1">MSNKNNTQIKISVTSFTQEKYITKYIIAIECSNQKWQIYKRYSEVEEIHNKLHELFNDLPQFPKKQLFHLNRSEIEFRMQQLDNYLNQLLSRKEILNSVIFRDFLILDQYDEFLHPIQTQHKILNMSLKDIHENDNITLLLLYDSSMMARIDTYINNILESKKQSPISTLICFSQNDFKQKLFSKEFLKALTCMNFNNTENAFAVGLSSGLVYYYRLDEKYTIDMEEQFQLAQTKIQGCVLIQRDIYAITSNFLKIQNTKKIQEQQEIPIGQHDLTNIIYNSNRNLIVISNNIGEIYLCQVPFVKIGLKVSTHSTQIIQLLIDNERNYLMALNHDPSHIIIFDYAKGLQNGFAQIKTNIVIKNKSLCFEWSKKRGEIFIGNEDGTISIWYVQDLQPFYVFKAHSKKINKIIWNENKSFLLTGSDDESLKIWYLPKKWRIGQADNFSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXC3</accession>
    <name>A0A8S1LXC3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ethanolaminephosphotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0450109</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXC3"/>
    <dbReference type="OMA" id="CTAAWIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF37">
        <property type="entry name" value="BB IN A BOXCAR, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8070201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="43524" checksum="26360C811D1553A3" modified="2022-10-12" version="1">MLQKYVKEQYFENLRKFKYNGQNFSILYNWILGDIAQFVVDHLPKTVAPNLITITGFCTLLSSFLLSLYLNPMFDQGLPQWASFYIALTIFIYQTLDNADGKQARRIQQSTALGMLMDHGSDCTAAWITGQLYLSAFKIAFTPFSMLAAIGVGFGGFFFGVYCQQHTGVFQLGVINGVDEGLPVIQLFFLITAIKSSEFWLNEIEILNGINLQYNTILLLITIIACVITIAQFCYPVFKKMNWNIITILNSLHLPIAVVFTFINLIYLSPTNVLSKWFHIYMLTIGLQWSKIINIWQLAIITKENFNQFSFTWLITLGAIILNLYSHYFTSDGFCYFDEVELIFCLLVFSLLSYLHCILSIVTQLCEILDIHAFSIKHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYF8</accession>
    <name>A0A8S1LYF8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0460253</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8071015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYF8"/>
    <dbReference type="OMA" id="KLYWSGK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="11"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8071015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="496" mass="58050" checksum="FECEC0A5DE8D0B91" modified="2022-10-12" version="1">MNNQKLINNTYIIKKRISSGSFGVVFLGQDINTRNFVAIKVDKEKKEESSLKREAEILRRLQHLKHIPKLYWSGKEGDSTYLVIQYLGRDLTHYIRTYKKFSLKCVLNIAEQMIIILESLHKHNVIHRDIKPENILVGKEDEENQLFIVDFGISKFYKEQNELHISYKEKQPFIGTTRYASSNAHKGLSLSRRDDMESLCYMLIYLIKGQLPWQNIQFTSEDDKINQVGQLKMNIDSNELCQGLPIEFTRYLDYVKGLQFKSEPNYKYCLSLFRKISNAHKYLSRDLIFDWIISQKTDRDGSQASSNHFNSKPSLFKKSKEDLLGSQLNQSIEQEMNPLIKMPDKKRTTLTPEINRKKNRLLSISSNNDTVSDNDFNNSIMIGIQPSMLSRLSKISFNSNSKFQNSKSNHSINANKNQFDHLISISPDSDFENILNEYKRKSQPNQSQNQILKEDDTTIEFQKMNEGEEGIERKYIELKLRFIRARFKPRLNNKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYI6</accession>
    <name>A0A8S1LYI6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TRAFD1/XAF1 zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0460051</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYI6"/>
    <dbReference type="OMA" id="DHIRDSC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051986">
        <property type="entry name" value="Innate_Immune_Apopt_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049439">
        <property type="entry name" value="TRAFD1-XIAF1_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16295:SF10">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16295">
        <property type="entry name" value="TRAF-TYPE ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21366">
        <property type="entry name" value="TRAFD1-XIAF1_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23580">
        <property type="entry name" value="Znf_XAF1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAFD1/XAF1 zinc finger" evidence="5">
        <location>
            <begin position="91"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="125"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="368"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="146"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="275"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="288"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8070613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="432" mass="50865" checksum="610FDC530B9186A7" modified="2022-10-12" version="1">MNEITIPCSNCKQPIAESKHMLHETYCLRNNIKCLKCGVFYDKNDPESHEEEYHKKEKCQYCQLESEDLKKHKCYKKPKQCMHCDLSFPADQLFQHENQCGSRTEKCDICNSFIIMREYSSHIKDCKPKPKVESSQVQKKPSNSEIYDRPNYDMSKIDDRIDQQKFNSKKETPQQNPSSQIQSKQSVQQQQQQQQQTKPIGSGYKFGQAAGEQKSKYQLSDEEKQRQKDGIRPPSSNNQTGSRPQQQNITSGTQSKQQLYNQKQQNSQKKDETTQFDNQYEKQQYINKLSQQSQPQPQQQQKQPTQAQKSLESYKNTQNDKQQFQNQNNNPILRRPDTAIAREIQRQIELEEEFGISSEEYLQQQMFNQMSLNEKKEQAQPIKKTTMPQSVIPARVPQKVDKQEFDFEDISEEEKLVQLQIMESFKDNKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYQ3</accession>
    <name>A0A8S1LYQ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0460086</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYQ3"/>
    <dbReference type="OMA" id="LDSKMMR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002216">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF160">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="21"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="149"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="49"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="201"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8070683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="509" mass="59953" checksum="FE559F523E9C5550" modified="2022-10-12" version="1">MFPIDLAKLATLQSKRLESDFKVLGRIGKGGFSKVYKVNNLLDKNTYALKKIELKAKDIKDSLEKNIERAQREAKYLARLSHPKIIRYFNSWVEVMQNKLDSKMMRKQEQDQNDFVIERNNFSFCDSQSNSRNDLENLIMFKDTQNEQISYSEQENEIQPKSRVSRKKQNHNTQNKKSTSQDEKKLEFDKIIFYIQTEFCQETLENYLQQRNNELLKLKKKNIEKYQTKLQKYEKEAKLILEQIIKGLDYLHNECKLVHRDLKPGNIFMNSPEDVKIGDFGLVTKLKQFYDFDDQDNDDDICTKMYAAPEQIIQNINKAFYDQKSDIYALGLIILLLFHPISTSMEMIKVINDARKGILPSILRDRHSKIAEIILECLNNDPKQRPNINEISFQDSSNSLSSSKKSSNEFSSDTTDYIIKNIGICQVKFEDEDIQEKYLIFNNRQIQIFKNQKSLKAQMIYNLNECNISYIDDKIVIQHSQLENFSFTTTHNYLQDIYDQLCELTQSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M2G8</accession>
    <name>A0A8S1M2G8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0520066</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0520067</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M2G8"/>
    <dbReference type="OMA" id="EHDLDIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="279"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8074077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="347" mass="39920" checksum="AADC13A02C4012C7" modified="2022-10-12" version="1">MRIGDESTSENDLDMYGISKSYSQTKLPNQSNNSKEQTQKNEIINKKIQSMRDDLQGSISLNDLNILGLQDYYLGSGDNHLETKPKINQENHESPGMDFQQNEQFNTTLDQLGSKLNEVIGHYLNELTNQPRLSLDSFSLQNSENLDEEQQQQILDFELQAMRQRIYKQIVDKINLSVIQSVQSFGSSCQLNSQSNIISTAFSKELINSTTHLSQISQITPPKTQSYSIFDLQQQLHQSIHCVQPKVNKKIEDQPIHITESETSKEELQNKLKISLSLNYQQQQVNNQLKEELSKLEKKNQISDNSEDGDQNPLLIENQRLREENGKLIEFIQNRICDQCKTIPKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3J2</accession>
    <name>A0A8S1M3J2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0440119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3J2"/>
    <dbReference type="OMA" id="QYKHEKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="31"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="311" mass="37897" checksum="86AB7E36171A6CA8" modified="2022-10-12" version="1">MASQKFMEFLSSNDKLRMNDMKLIEQEQYYINQLKDQREKMELLKIEFLKKDHVILELQGHLEQLQDECEQLREIYQEHVQNQENEFYRFNKLQVEKDNLIKDNEELKDVIQHLRNENETLNSLQFKNDDQQRQLQEQLVLLAEENARLKQEIAKLDELLFTYEPLKEENEKLNEKLRYYKHEKHKLSQELKQGKQEINKQIEEFKSGYVKDLKNEIQRLQEYKDQYQSLKIQIQDLEQKYIEVINENNDLKLEIKSLYEKEESEIKIKSEIDRVRQDLMSVRYQETLKLNELFNGMISLSSLANSRDHFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M7U7</accession>
    <name>A0A8S1M7U7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0520010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8073963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M7U7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="186" mass="22936" checksum="CF3AA7B31611C14E" modified="2022-10-12" version="1">MLLLSFMSYAKNIFIQKMNRNYLKCQIPFRQYQTIQLKFIIKTIIFRCQLRIKIMQNYIILSWDIRSYCSNFKSLLHIQQIRLFKYSNTYDRITLQVWNNQERLQVMSQNSRKQITGNQNLRRYAYQFFYYKKNPCIFTEIKNFPFNDRYIIGISDNPFSLMLRNIRDMCAMMDFINYKRMPGYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M971</accession>
    <name>A0A8S1M971_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0480080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M971"/>
    <dbReference type="OMA" id="FRCECDC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362:SF309">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="22"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="5">
        <location>
            <begin position="919"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="332"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="101"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="344"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="362"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8071844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="976" mass="114462" checksum="D0D64BA233904FE0" modified="2022-10-12" version="1">MDLSSQLNQMKFIKGLDFRKNLIDNGHLGQGNNMVYNVSVNGKMYALKQVIMNSSNIENEIKIMQSLSNSQYVINLIDYMILETVSQDKYTQNQQFVYLLMEKGQQNLEQYLKQREQQYLEVEELHKILEQLLNIFEDLQQKNIAHRDIKLVNILIMEPFQLKACDVGCSSQIETIQTISVVGTKSYLAPELLNAETHRLKHNPFKSDVYSLGLCFLYIVTLQLWNTRQIVSSQIEEEIIADYLRHVKRITADDNIIISILKKMLQINPNNRPDFIELKQIYIQLKENQNQKTNLSKGTTFESFESPKKTPLQFDYLSPDIVVNRSRNTGSNFYKEERQKSVRNLPSNLSNSKKQKSQANLLSETTRKQQINKSPKNQSTTNLQLKDTQQFFSNKPPTPNSLKFTQSTKAKSFQSSGSPNHTQTNFQQRQFSKFHQSVIENKKVHTESFSQTMPTIQTSMLAELKPYDDFLYCNYEDLNNLYQFQNYVCLFSNGQKSNIKKCQLPNSCKLLNLKLNNQTPELHLSTINDIQILILELIDDCNEKNLNYKWSENLGSMYFQAITSLEIIVNTQQPQTNNIIQFLTKIQHIPKIKLIYRSEISEQDHRTIMWKLSKYNCTSFDFRIPNIKLNLTQISQQWQFNQSLEELSLDYEDCDIHQTFSFLVTSIRTVLFKKFYINLTKLQSKNSEISLMLEYLGSKYLLKDLTLIMDQITNYDKMVQEKLKHNISKLKNLEKLYISIIGNNINFEFFEKLFNIFEQLKELKIIILRIDRIFEKQKIRELKKIFPFLNPFILQFYGTNKTLTIIMHNNEVKKLQDYGTTKCNQGKQVKLSQMYECLKCSKAHSFFLKHQFQICSTCVTNCHSKCDNFNLNNNEAITVSSDSQLQCQNIVNIIKKNHTFFESNDTRDFKCYCNLLGTCTQAICNQDRNRNEIQYKCKTCNKIICRMCSLDCHSCHTSKEMYINNFRCECDCDKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MB84</accession>
    <name>A0A8S1MB84_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8075003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MB84"/>
    <dbReference type="OMA" id="GFCELEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="49"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="303" mass="35913" checksum="686A24AB10322C63" modified="2022-10-12" version="1">MQQNITCLDHGKQMEYLNFYVNSKQNLFCEECIKTQNIQHTVVRIDKIRDKVINELKSIKQQQEKYQNKVRIIQNSLISLKEKEQQLCKVIDDIDIYQFQNIQDYIQKFYRINNGDIQTTFNQQFFQDTKPIDQLQRIYTFSNEYKNDSIILLNKFTIQQNKVIMNSLAVIDPILDQQQESSIYFKINHIGQSVAIGFCELEVIKQQQYQISNWNSLGHGCYLISSGGYTYNSKDWKFNFKKIGFCFNQNQIIKISYDPLNNLIIFEKLNESKSQIEMNINAQNLYACVYVSKPKDEIEILNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDC4</accession>
    <name>A0A8S1MDC4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0600095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDC4"/>
    <dbReference type="OMA" id="CSSPMIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8078530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="370" mass="44232" checksum="478F14D98BA12ABD" modified="2022-10-12" version="1">MKNFLEKYINNDYSIRMNNVSQNHEYQLNQQKCSSPMIFGNRFASPSPFRSVTPIQGQLSPANQSRRESAFSRNSDNYDFSVKEKSKIVNKAIIQLFQKQQEYQNEEECTIDYFFDKEQIILEEQELLHAKLRIIRDDFNFKIQQLEDFLREYLRERQSILQNLSISIKEEQDRFNEQSKQNQRKNKQNEIHTRTDSLNKHDSKGDSLPIQQEILRVGSSMKEQRIDSNLEKRDGFDIRRESRHESINGCSSHHRRESSYKTVKLRESRVKREESLKCDSVKDEQPLKSALKKKDSNRQTYNCQDDSQDDWKFIKEAKNNLEKQKTQLDDKQSPNKVRFVNQEKSQKKQQEKVVKSKYLNDIDIFLKCRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ME29</accession>
    <name>A0A8S1ME29_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ME29"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="47" mass="5573" checksum="F94A9E8A7EEA877F" modified="2022-10-12" version="1">MDSRLIRPSTPKKPFDPTLLRQSAKKPFQLRTQQPNILVIENYYLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MIF4</accession>
    <name>A0A8S1MIF4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0630201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MIF4"/>
    <dbReference type="OMA" id="NDFQNEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="140" mass="16456" checksum="0FF7984683CDC25F" modified="2022-10-12" version="1">MNKLPYQPLGFVLKFDPPIIGLLYHPVENKKVNKKKKKCYAIHLNNLIFLVEPEDIVEALFNEHQEFLDPDVVKPQQVYKLVLRLVAYRDHQLRNMQEMGEDMADGYYEEEEEEPVQQPKGKGGKAQAQQRRTNDNNRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJG2</accession>
    <name>A0A8S1MJG2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UFSP1/2/DUB catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0610098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012462">
        <property type="entry name" value="UFSP1/2_DUB_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07910">
        <property type="entry name" value="Peptidase_C78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UFSP1/2/DUB catalytic" evidence="2">
        <location>
            <begin position="43"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8079052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="287" mass="34475" checksum="5F0988421B28CC11" modified="2022-10-12" version="1">MKKIRNELFHTIIDQKRCLLNSIYFRIRKYFHFSSQIFRTIQTIIYXYFSDQIDDAGWGCAWRSIQMMLSSQYNITPEFRWLFSFFGSKTILEKIYMHMNQLDQVPQYLSNSVYAPHDLANGWAEPYIGHLILYYMGFESKLFLINGYPPNSNAPKEVFTETQTFTQFQQFLQEHFLLRKSPIQIDDSQYSMVIHDYKIDGNNTILFIGDPHIYYIQNNHQKIGLYTVTLNEKGDQIANSITPDQEQFYMYTNSYKTLHFNEKYWMATCIMKSQDVWLDWRERQLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MK97</accession>
    <name>A0A8S1MK97_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Saccharopine dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0640151</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MK97"/>
    <dbReference type="OMA" id="YISPALW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004753">
        <property type="term" value="F:saccharopine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="2">
        <location>
            <begin position="16"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase-like C-terminal" evidence="3">
        <location>
            <begin position="135"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8080760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="56180" checksum="82A45C56DAF097D2" modified="2022-10-12" version="1">MLSLYRNQLIGQQKRILLIGSGLMAEAVIDQLLKRNDNFVVVASAHVEDARKVIQNKERCSAHHLDVTETEELRKFVKNSDIVIAYIPPQFIVPIAKICAEVGKSMITSQYTFPEIRALEEECKKKGIIMLNEIGLDPGIDHLATVKVRDEVYAKGGKIIEYESWCGGVPSPEFCDNPFGYKFSWSPFAAIRNINNDAKYLENGVQKYIPASELLYSTEIIHVNPSLQMEGYPNRDSLPYQELYGLKDCQKLVRGTLRYQGHCVLMAAMKSLGFASEDIIKVDREMSWFEYLLSNIRFESCSTNYLANHHITQLANTIDQKVFTLAQLETLLTKVFNRVFSQYYYKDKSEEQLYKDAEQITYTLKWMGIFDPKNLILNNVTHVHNFAAHLQTLMNYRQGETDLVAMQHIFKIVYPNDPRIYVKKSTMVKIGHRNGKSAMAITVGVPTAVATQLILDGVIKVTGVHMPNISEINTPLYEELKKEGIYCEEEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKT8</accession>
    <name>A0A8S1MKT8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0330233</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0580117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKT8"/>
    <dbReference type="OMA" id="ARMIETH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:001016">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8062629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="16557" checksum="13220D52B0F5A273" modified="2022-10-12" version="1">MSAPFYTVKDVPAQDFIRAYAEYLKKNNKIKIPEWASIVKTGLGQEISPIDQDWMYVRAAALARKIYVRGHWGVGNLTHMFGSVNDNGKHESGSGKVIRYLLQQLESIKVLKKDNKSLLKKGSRIVTKEGQQDLNRIATQVALAARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLS4</accession>
    <name>A0A8S1MLS4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0590211</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLS4"/>
    <dbReference type="OMA" id="LATNCEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004753">
        <property type="term" value="F:saccharopine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12189">
        <property type="entry name" value="LKR_SDH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000087">
        <property type="entry name" value="alpha-aminoadipic semialdehyde synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007545">
        <property type="entry name" value="LOR/SDH_bifunc_enz_cons_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04455">
        <property type="entry name" value="Saccharop_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal" evidence="3">
        <location>
            <begin position="7"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8078193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="547" mass="62853" checksum="25A9CAA118B6BF63" modified="2022-10-12" version="1">MDKLYIGIRAEDKSYWERRTPIPPHDCKYIMEKHPQIQIIVQPSTKRVFTDEQYLEVGCLVQEDLSQCKAIVCIKEIPLDKYIEGMTYLHWSHTIKAQPHNMPALDMMLKKNIRHLDYERIYDEKGVNTTAFPYAGIAGVITFMNEYGKYLLKRDIATPFLQIGPTYQYFNKKDAYQALNVAGQAIKDRGLPKEAGLPIIIGVLGSSGLCGKGSMEALQNLHITLVKPSELKELVNTPNDPKHRRTVYVCPFKTTDLVRHQEDYDKEFTSQDYYNHPNQYTPVFHTKYLPYLTIIINDIYWDHKFPRYITNSQMKDLVQSGKSRLQAVCDVTCDMEGSIQFLKKYTTPDNPVYFYEPISESIHDEFDAKSPKDIMYMSIDFLPSQLPYDASIDFGIALRDIVPHLAYSDSNKPLEEQGLPVFLQNAMITLHGQLTHKFQYITHLRNINDQLKEAESFQPKKALKKVPSYLAIKLTGHLFDTGAINKILSVVQSGCKFNIVDIQAGQSDNQQTSCLLQLYSKNKGQLMDEVDKVIQLCETLDIQVDME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLW1</accession>
    <name>A0A8S1MLW1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0640066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLW1"/>
    <dbReference type="OMA" id="NIVEITY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053299">
        <property type="entry name" value="ASTRA_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44156">
        <property type="entry name" value="SUPERNUMERARY LIMBS, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="400"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1186" mass="139004" checksum="3F8AD4DB7B2B4CC1" modified="2022-10-12" version="1">MNNNDEIIQKLDVIVANYLNQRDHNGQLLTEKYSEAYIYSLVQKHLFDYEAIFTASQKSVIDVFDFITSLLKFTSEPIEDTLFIIVQAIDFFKAICEEYQTYTIKFCDLSSYLCKYNPNLDDKDVPLQNRTITYHKYPPLKQFNKPQSRSVRSIDLEAPIILGTLGHYIKRINKDKVYIDSQKNQNSSILRMQYQESESKILILHQYSDYISIYNPDCTLLSRLRPKAQQLRNIIHDFCWSEEQQRIGILFKDAWICFMDQSDQFELEKYFTTKIDQNRIYYVQNKWMTVGTDSKISIWNLETENSSVISHDQIKNVVNIVEITYLQLVCIACQDKLTMWDLWEYNKPKLLFKLQVNHSRLNNIVFFQEYHLIITSGFDTKVNLYQLHKKYNDGDYVGQLEGHNAIVTAIQCVEKSPIVITTDDRLIVKLWDIRIMKCIQSFDLEMKQSINYITIMQNLSSVCFATNRLIVMAFEGHNKNLSQPKPQKQRTIHLYPKQVELNAIKKQLIVSTNRDIRIINLSNGQTDTIIKAMIGQTDDMINQFKPIQQYKKIVLGTTKGLIKIFDTNSGDLIKEYQNNSNKITHLSIDYQNRIISTSSLDGQLHIYKENNLNLESLRKAENVHNGIDYCYVSLFHDLIITAGVDIVCLWNLEFTKPVSTIRTESPPKGVYILDGYRILIIGCKINTYFLQFNKSEDQYFYQQLGFIQQSCCTFTDKNGAVLLGSRRGLICKLQFNTQKSQKVQFHPFRQNWEDYNLGVNNHKVTRFVISTTHELKLEQYFEAHKKRIQHIDFISLEGDNLLKEGQSKVISTSKDGFLKIFDYPSFELMCALNVNHILPLKWSIIIDEHEILKFKMAFALKVIHFIRSQPNLTISHLRMLEPNSIFKNLLGQQKFFKQNINDNKVQLLQDFYEPRDLVFQKVKGFYKSELQGLSLKQIEEKKKVLLQAKMFEKDYTEQNFQSEQKQNEDIGNKEFKSWQQFLHPNFRKNGVQGEIEQIERLHMIHQFEKKLDQVLTKEDETNIKPNISGRRYSKTKQDIQNKSANKSTRLRATLPFFQYASDRKLASIPASTDTSQRSTHIGYDTMRVEYSKKKFQRVQFLTQNIGQLISDLDQNKKKQGLEIGENAMRKTSGQVFNQIVNNLNEKLRDSKFKKYSRSMCKQDLEGILIDAHLNSIHSKQSLRRIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQB7</accession>
    <name>A0A8S1MQB7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0610264</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a fundamental role in microtubule organizing center structure and function. Component of the infraciliary lattice (ICL) and the ciliary basal bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the centrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQB7"/>
    <dbReference type="OMA" id="KTRMPRE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000001">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF59">
        <property type="entry name" value="EF-HAND SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="80"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="153"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8079323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="190" mass="21826" checksum="B8343540B6D14BB0" modified="2022-10-12" version="1">MSRQAPSSAQKNDKKAPPPPPPQKGPKPFNAEDYAKPPHLTKEEVLEVKQAFDIFDNDGSGSIDPQELREAFEASGIKTYHNKFIYQVLGELDTDNSGGIDFEEFLHLATAKVSDKDTREQIQKVFNLYDWNKEGRITWDELKRVAQDLGEEMTDEEIQHMFKKADLDDDGFVTFDDFYNLMTQKEYGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSU2</accession>
    <name>A0A8S1MSU2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase-like domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0670231</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSU2"/>
    <dbReference type="OMA" id="DDNIMSA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000737">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="10"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="292"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="328"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="527"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="563"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="398"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="615"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="792"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="615"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="631"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="660"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="690"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="702"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="725"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8082392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="869" mass="102034" checksum="F0071CD27DB9200C" modified="2022-10-12" version="1">MSRNIGDFTFNSNECIGQGTLGKLHKGTNKKNNQDVVLRLLQKSLLKDDNIMSAALTEEMALMQKLKHPKINPILEVIESQTFFYVVCPYRQSNLQTILQKQRKLPEAETIKLINDLIDGFSELIKQGIIHRDLRPSNILYDNGSYIISDPGFLKTIESFKKDNKTKPQQFYYSAPQVLENKSKYSNKCDIWSLGVIIYECLTGVLPFPTSSLDEYLKSCRSKKVEFQQGSISGGMKSFIEGCLVYQEEKRFDWTKVLSHPIINAKLQNKNQDIVKDNKKYLMTQIRQKIIKKNLDLQDVYQKIDANHNGLLDFDEFTTFISMIDKKVTKEDCYYIFQQIDKDGSNSISFDEFKLWISSNNTRMAFFKINKPDNLQNNKLTQQPKNINQQQEFIFQQQQNQIKQQTPQQQQQMNPFQSPLNQQQQQQQQQQQQQQQQQQQLIQQQPINYQQQQYQQQPIGYQQPQNQFPINQNLPLQQQFYPQPQPQTQVFPQTQMKSSGSIPAGAGFGRSDKLERTILMIRNVIKSKQMDVLTLFQQFDFDKDNFLNFQEFSQLIWSLNNQLTVEEMADLFKVFNLNSDEYVSFVEFRLVASMANDKKQNLQYLPMYQPIQQNNNFQQFNPPFQGYQNPYQNPAQSPNIPMIGNNQQPIQNNYQMYNDQYFTPQPQQNNYPTTQLGNNNNNKGIRKQNDASQLDILSTKQAKKDNKKKEREEREAKRKQQHSFSPKEEKPKKHLEEPLRPPMLTKQDKQQSILETPKYPTEKIGSQNKIPSPHPSPRQDKRKVEQPTVTYAQTKNQEENQNQQKASPQTWYQPKYMTDYNYTTIGSKMDQELAESTNLIQNSSIPNFNNKPYRKVLDDKIDKFYYSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MW19</accession>
    <name>A0A8S1MW19_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosducin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0660074</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosducin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MW19"/>
    <dbReference type="OMA" id="VQYRYGN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051498">
        <property type="entry name" value="Phosducin-like_chap/apop_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024253">
        <property type="entry name" value="Phosducin_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45809">
        <property type="entry name" value="VIRAL IAP-ASSOCIATED FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45809:SF3">
        <property type="entry name" value="VIRAL IAP-ASSOCIATED FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02114">
        <property type="entry name" value="Phosducin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosducin" evidence="2">
        <location>
            <begin position="50"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8081596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="235" mass="27930" checksum="B249926019655EC4" modified="2022-10-12" version="1">MANGVTTEWEDIHVKHGNYVEREKVTTLAETYQQAIQKLDEKQDSDDDFFEDDEFTKQYKNQRLQQMMEQAQKKLYGEVYEISRDEYVKQVTEASKDRFVVLSLYQDYIIESLKLNEVLQELAKQYKEIKFVKMVATKCIENFPDDQCPCFIIYKDTKAVSNIPFIHKLRKITFASIEDLLISQGVIPKPVEEDEQYEEIKDKLKHPYKTNNVKDVDDDIDDREYSHKVQFQKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWJ7</accession>
    <name>A0A8S1MWJ7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA repair nuclease/redox regulator APEX1</fullName>
            <shortName evidence="8">APEN</shortName>
            <shortName evidence="8">REF-1</shortName>
            <ecNumber evidence="8">3.1.11.2</ecNumber>
            <ecNumber evidence="8">3.1.21.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">APEX nuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Apurinic-apyrimidinic endonuclease 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Redox factor-1</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName evidence="8">DNA repair nuclease/redox regulator APEX1, mitochondrial</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0610188</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6 8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6 8">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="8"/>
    <dbReference type="EC" id="3.1.21.-" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWJ7"/>
    <dbReference type="OMA" id="GWRIDYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09087">
        <property type="entry name" value="Ape1-like_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000130">
        <property type="entry name" value="DNA-(apurinic or apyrimidinic site) lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00195">
        <property type="entry name" value="exoDNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF6">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00727">
        <property type="entry name" value="AP_NUCLEASE_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 6 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="10">
        <location>
            <begin position="91"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="38"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="7">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="7">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8079224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="348" mass="40040" checksum="29627734A6D2FC82" modified="2022-10-12" version="1">MDQRRGRSGNKKNSPEKNDEAQEDFVVKSKGKQQKGTKQTEDNEEKKGVQKIEKQQNIKGVEKKVKQINQAEQKYELGDGKFSTPTIKISSWNINGIRAVTKHEEAVKYLSDNKFDVICLNELKADQIVFDKENLGAKFGKHYFLYLNFCKSKGGYSGVAIASKVKPISVKCDIGVDKHDQEGRTLTAEFEKFYLVSCYVPNSGQKLERLDYRTKEWDVDFQNYLEELRKKKPTILCGDLNVSHHEIDLANPAGNKKTAGFTQQERDQFSNYLQKGWVDSFRHLHPKEVKYSFFSARNNCRQENKGWRLDYFIINKEASDAIIVSDINLTVHGSDHVPIECEVDLRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MX66</accession>
    <name>A0A8S1MX66_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0730010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8084830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MX66"/>
    <dbReference type="OMA" id="YWNDGSK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007286">
        <property type="term" value="P:spermatid development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="43641" checksum="49CA58DFB055ADAD" modified="2022-10-12" version="1">MGNCCGQSSIMECHSQQIVTKDQMDAEQANKAALKIQSYVRGYLTRKKHKHHHKNSSVAITCTNEYASSKKQSRQEIQLVDLKKFNSFSKETNVPQNLKTIEKVPETLDNLTKQLRKGLPEFIYDACQSEPDLITRGPVQDEHDHIYIGQWKDNAKWGKGIQYWSDGSYYEGYWKDNKQNGKGRLIHVEGDVYEGDWKDDKAHGYGKLTTSSGGYYVGQWINDKQNGQGKELWPDGASFEGNYVDGIKFGQGVYTVANGSQYVGEFAENCFCGYGTYKCSDGTKYIGYWKQNKMNGQGEFYWNDGSKYIGEFLNDLRDGYGTFYYGDGRIYKGQWKNGKRHGNGTYIGKNQIEKQGKWQNGVFVVWEKQFTNQSGGDDPLFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MYC5</accession>
    <name>A0A8S1MYC5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MYC5"/>
    <dbReference type="OMA" id="INRACMK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="115" mass="13581" checksum="1914D6742EA0E8F0" modified="2022-10-12" version="1">MDSQQIDCPLSELMTNEEIREWAFSLDPQPEKKSKLNSSMPQISSSATINRACQKQQEFKTKQVPRIIQTPQQAPFQLENQKQKKEIIHRLKLQNRLMKEMIQQQKDLLEQMKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZX0</accession>
    <name>A0A8S1MZX0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0630257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZX0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="4"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="151" mass="18169" checksum="FEEF57E879D8A2B8" modified="2022-10-12" version="1">MIVNKHLSIQNKKYLCQLRKAQNQVKFAKLSLKKQLLQYIYPIVLMNHLENKNQNDLLYAQHLDFQNQFTKLYQPIKLHHHIYQAFVDYQLIKRMFIINTFSIQQIHMLGINFQRFISPQNCIIIFTKHSQAISQQKESLLQLHLIYNKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1Z0</accession>
    <name>A0A8S1N1Z0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0700225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1Z0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="122" mass="14246" checksum="D7A845BE071F608C" modified="2022-10-12" version="1">MLLMDWKILYSEIIQNKAASSDTKFLAQVAKSGFCKLMKVDVFLDLHVFFIEVKQLLKRVKLRSQVSLIQQLKQQQELNHTNLKNTQKSKHLQKTLIFLMNSILQNQVTCACYKNKLSCKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5S0</accession>
    <name>A0A8S1N5S0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0790127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5S0"/>
    <dbReference type="OMA" id="ECYKYEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="325" mass="38734" checksum="DC1170238371C468" modified="2022-10-12" version="1">MDESLFQNNFFNKQEICQYFGNVLLATDYSGQIVLIFCNGEIITYALVQEKQWQQTSSLKVIPQISIIKQNDYSLQLNGNGRRFIFMQVDQRYELKIQIWQRRHKFNWTFQQSLKLNSRSKFLSINLNGSMLMVDHLKTIIIWDYNPPQTKLVRVQSVPLEANTACFNDNDCVIAAKSKQHVVLIKKVNHIWVKYQSIKGEQEFFMQFFKNRLIIMSIYDLFMYKMDDSFHFSIEKQIISGFFTKEPQQCLFLGSIVLIQMPSLQVECYKYENNVWLKDKTYFSSKFEAHQILFSKDNKKLFIYRKKYGCIVFDKKNEIKEKIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NB64</accession>
    <name>A0A8S1NB64_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NB64"/>
    <dbReference type="OMA" id="TIQCGEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="6"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="278" mass="32363" checksum="4A63094E257582F4" modified="2022-10-12" version="1">MKSDYLQLFTKPSTIPNSGLGVFAKNDLTKGQILVEYRGKIINAKYSWAQCFMLEDRMLQINEKYSVIGRSLSSRFNDIVRFGLLTPMEEQSLNFGVFPIHEHLKHNVELSIKHDKAFLVASKNVKAGEELFFDYSFSYWKSFYDQMKAYKGENFFDYWEQQKQKPIPEHYIICDSDMLKLQIIDGKVYSKSTIQCGEIIIECRGKYFETEQPGRINLKLDNLFLRLNRLNNAILQNKLQSKTDLNAELVLIDKKFFIKALTIIAKGEAIFVDNNNQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJT4</accession>
    <name>A0A8S1NJT4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0900110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJT4"/>
    <dbReference type="OMA" id="QQEDITM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051942">
        <property type="entry name" value="DENN_domain_containing_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288:SF0">
        <property type="entry name" value="UDENN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="2">
        <location>
            <begin position="108"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8092312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="607" mass="71475" checksum="D489D6231A606692" modified="2022-10-12" version="1">MNQECVECQKKDQKIKELKQKIAELEEQIETFGPEFSQVKGLDENSQKLLKENIQKAIGDLIGQDPNKLKTNQQKAPFQRTQTSNLTEFLRQPNIDEQHTYLGQRFFEHFLIIGPDLEELQQVNKHTNEIIVKPKILYQFPNVNEHLYDVVPIYTFPNGILAKKLETSVHNFNDKVLPELKQIIMQTLQLTKGDNEYSITIPREDIQDNSRMTVSEFVQESNPNVFRNIICFEINEFICLDPKTYGCWLCPIVHCFVSYYPFIKFFLKENANIWNQLKLERIEPCLSETSSHEVLKRDFIYMKNKLNTIIDRLFALRSIQVTYDQKYQLNEIQIQTPKKDSAQYLQFLWGCDQTFQNLSFKELIYLISHVLLSQRVILFCKKKSVLSHIMLTIISLMHPFNYKLPFCIFIPDELIDRLAAPCPYFFGVVGEYDNYCQRIEEAQQEDITMFDINTKRVYFSKNQSRDLKKCIAPIKRNSHLKQLYSIINNSKENAFKQIGKHKLQKLEKSGSSNQIFKENEKQYMQLLKDIAKTFQTFWHSTVTQHIPVDRKFIKTGSMAALNLQLLSEVMLSKCPKDSKRFIKELIQNPTFTGYLDELYEGIYQQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NNB1</accession>
    <name>A0A8S1NNB1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0970012</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NNB1"/>
    <dbReference type="OMA" id="FFIECAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051581">
        <property type="entry name" value="Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524">
        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524:SF6">
        <property type="entry name" value="CALCYPHOSINE LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="263"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="397"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8094747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="425" mass="49984" checksum="FB95A3A88B1477AD" modified="2022-10-12" version="1">MHLCDFFIECAQIEQKIEQNRQFLIQLPTFHPYKAFIELQDGNNKITTTSLSSYLKSQGLLCTEQECSIFIQKPFKYVDFLRTILPTDQTLRKVISIQEPKQQLSPETQLALARHLNLQIDLMNSLKTSKNVFFEGTIQDVQKYFKDNLAYISDKELEQISRLLDQQGEGIISTQQFIKKKNEEYDFTFSTPKQVKNNLQSMTTQKKKEQKLTYNGRSNKKPIQDLNNRDDNYLSIESPLIPLVALMRREIKLNSLKKRLLSQKTFNFLDAFKALDQNSNGYITMQDYDKFLDQQQGSSKYLFQQKKELQFSDFLNLMMLNENIQISNKLFVDKPIEEIFSNQTLQLFKEYLELLQTPPPKLNGSKDLFKLIDSVTRDNYISSTDLQQYLYMNGLRYTASEVSLLINRYDHNRDGKLCFFEFQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQN6</accession>
    <name>A0A8S1NQN6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0940016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQN6"/>
    <dbReference type="OMA" id="CYAIGFN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="331"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="375"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="553" mass="65472" checksum="BD091C77ADCA96C7" modified="2022-10-12" version="1">MKSMLGRCRMIEDLDKITCIGQHNDKISLLVMLENDLQNEQRLYCEKCLQTKKGDLNTISFDEAKLRIEKQQNRHFSELNYSIKQRIKFIDEFSENIIRVLQKIDQFIKDSEQRIKLWKDQLIKFRDDNYTYSLIQELSNLNKEENQWVQEKYLDSQNKINLIDQDHSLHLKFMVDEIQHQVQNIVKAHELLYANIIIHFQNLINQSQKEQEQEQLKKENIQLSQRFFNKLFNKLVQEIRQNEICYAIGFNQNDSIMAAGFNECIKIWKIENGQFIDSQIILKGKSEVDVAICITFSKRKNLFVYGSYDHNIRIWNEIENGQWNPSDSSNEMSHSGMVTCILFNQCEDQVISGSQDKSIIIWKFNGTSIKFDQQLIMHKNTILNICMNQQETEIASMSKDQQIIIWAKISNKLKIKQIIQQSQINFGDIITFISNDVLVLYKNFQKFTYVRSSIDNHFSQISQQSLDLTDIVKNDEQYLFPTFHFIHNNLIVQKKDGNVYFVVVDEDFQPISLSKLYINNKNTFYGNITNNGRYLVIWSFGDLEFKIYKLSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NR31</accession>
    <name>A0A8S1NR31_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0940098</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NR31"/>
    <dbReference type="OMA" id="MWPWCER"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00299">
        <property type="entry name" value="GST_C_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050983">
        <property type="entry name" value="GST_Omega/HSP26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="88"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8093869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="28160" checksum="602CC85B06133344" modified="2022-10-12" version="1">MDKRFKHTLVAFKICPYCMKVLTIMCHKNVKFEIKFIEMHNKPDWFLKISPLEKVPILIIGEEVVLFESAAIMEYIDEITPPKLMSEDPIQKALDRAKFEYSNEIIKSLYQLIFSTEQEKFVKIKEWIIKRLKQMEEWLKDKKYINGNELSLVDLNFVPVFVVLNMLKPILLCDIMKDYKRLQNYGDSITSLPCAKTGRVPDYEFLMIDGIKNQNTVLFRSNPCYFNGPKSSHCCFFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUF3</accession>
    <name>A0A8S1NUF3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0990169</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NUF3"/>
    <dbReference type="OMA" id="HRKWAFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="108"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="166"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="228"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="228"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8095822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="513" mass="61286" checksum="B7B8861575974C0F" modified="2022-10-12" version="1">MVQYKYQLIFLLVNFRMQTRNWRKKKQILEKLTRKNEAITQAKNDQQLFDSNREFFQKFQNFYENVQDIIIPSLQFLQEQFHISELLTDQQVYQLQKDLHEQWIPKLRKNWTDQDKQILIWIVLKICSRDGINIRKIPNKIWEEVVQLISRRTVEQCKNKWTDLLKLSLQQIPWMEEQDIYLIDLIRKSREEGKQNKWCTLANLLNQQFKESPRTGKQCRERWNNHLNPDINRHPWNLEDDIELLEIVKKHQRKWALISKTLKTKRSENAVKNRFNCLLKKNHCTSITTLINILKNRFKTEHPTIPLSALKKQMMQKYYLKDQDHLKKQQDINQLKNQTLFTIEIFDNHTLSNLNNLQPAFYNSKTQTIIMSTNAQLQTFLNNQKIKVENDLNINFISEMNIQQPEFSNHGSSILNIIDSSNCVRYVPCVIPNLGYQQQQKVDKKSQFNIPGLITQNNQQSALLRNKNCSSFTFFSNQQQNESTEKSIQNNSPSEQQDQSTINKLQIEFTLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWD1</accession>
    <name>A0A8S1NWD1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0990041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWD1"/>
    <dbReference type="OMA" id="MSRIYDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="613"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="633"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1019" mass="121191" checksum="44A31775DDD6FCAB" modified="2022-10-12" version="1">MLLYKALQTEDEYERLQLLSEGIKEVTSEMPKIETIQSTALIKFLICCYPYQEQNINMLISAFFDYYKNENFFWAYTQAISQIGDYQESKAAAYKKQSIFDVYLRKQKEGKTGLNHFYHFSESVQQQMTKVKLYVCSYFSKYFSLAQDLGIDMSRIYDIYRIYLHNTSNKELIFELIESFENLKRTLFNNLPWIDTFQSEIDIYKQTLIPQRFSVKFKQNQHNLQQLYEQYKQQMENPGLQNQNSEENSPQVDQKAVYKQQIDKYQIALCKQNLVNLIDDSIDLYLFLKLIIKPQKYDLIDDWNVKYSETTIDKDYQGFKINCSELDDLLIIRSYKALNSVLNCTKFEILKLKMQNLLPKIFHLIYKVLRDPEQNINLYHLGSLINKLLEMCPKHTIYQIIKLNLLFSFTQFFYNNSIVNLVLEILDFDTDKYQLGYFVQEQVWTYINDTDWFQYLFTFLFKQNIDVSKKDENYQSERKTQVLSMLQSLNRNQRGSIKQQVTVNQSYFVQCYSTSEKLDELQPHDFLNNRDIDNLKFFNNDKLNFDPKIMEKMIEEQKKNQVTSGQNVMIEDQNIISQRVNKYGRQMIEIPPKKKQVSLPKLNTISVKSPLKNINSPLSRESSKNLSIDKDAQLSQKSKQSSSSLRPSDNQDDTLSQNKLIRIYQATSKTNASLFKQNQQGIQIKDIGLMESLQFIYRLLNSLQQFQKKFLSHKNCNIVEKTSKILTTENLVQFFQIFVSKIMKDDYGLICGQILNSIYQMVLNEHLDVYYEMLNEQFYNFIQEIGSQFILLEKQQNTGFKRYLISSIFFNGFILNNYLGQNYLEFNIYKYLSSKIFNQLQQYLINCPENNNYQLQFTKFLNCVFSKAPAYMIQQILFNYGLLQILFESQRVIILNDVQNVQSSLHYFAIQIIYLIKDLLNKRSMQVILDNLQPLDLWYKLCKATENFKIVNLDQIKLAEPPQEIRQFSKKQQRQFQFKKQQTEQKLEISERSPTELCLTPDLTHRTLKNYRLRKPFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWE5</accession>
    <name>A0A8S1NWE5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0940100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWE5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8093873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="112" mass="13580" checksum="437E33611DA55BE2" modified="2022-10-12" version="1">MIKNKVSELDIKNDKLKDKVEIINDLYEQEYINCQSKQKTQQILYLNYLKLIYRCGKLQNKVAQNDIKILELQNALKYRDDQITQNEQRIQKNWKQIQNLKKIKQSKNIMSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWH9</accession>
    <name>A0A8S1NWH9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0940096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWH9"/>
    <dbReference type="OMA" id="SSIMIIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="69638" checksum="B7EC280B23210F31" modified="2022-10-12" version="1">MLYSIQKGLKRIDIFGQTITLNMNKNAYYTTSFGGCSSIMIICLLSLIFYSNIADFFTKANVYYNSQTAFSNDPNYMKMNDNNAMFALSIDQNNYTKLPFFNITMEQKIYERSLNGTIKKSTIEIPLEPCTLNRFNNVVEQQGIDTNFNDSFNYLGMDQWLCPRINYQLELQGTYSSETFKFIKIIVSDCQNNTNSSYWNPVCANETLKSQYLNANGQFKLQVFQINTMVNPQKAKNHKTMYLDSDMYFSFVPNQLSRLANVYYRSYVINNDQSLLPYEDIQKEELIVRKAEDFRDLTELGRDTDSIYATIYLRRSPFTEIIDRNYEKLGDLLSYLGGFMQIFRVIFGFFIAFYNRTSMLIELSNRLYDFKEVTSRSYIRKGAFSQNLGEVTTPPDEMNIILRNQPNNDLQKVEWKQFIHKLVERSQPIKLNLKILINELSCGYFFNNTNSSFFTKAMMKINNELDLHNILHQLQEVSKLKTVLLQKPQQILFNFSPKPIITLNQENNVPSRIELDFYNRQSYIRNKEEIPNEELFTQLQDAYKTVQEEMENSQNSLICQKTINFKLTKQIDDELGKFLRSRRMSCINQEEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZU9</accession>
    <name>A0A8S1NZU9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Maestro/Maestro-like HEAT-repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1010084</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YHLDRIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055406">
        <property type="entry name" value="HEAT_Maestro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045206">
        <property type="entry name" value="Maestro_heat-like_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23120:SF0">
        <property type="entry name" value="MAESTRO HEAT-LIKE REPEAT FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23120">
        <property type="entry name" value="MAESTRO-RELATED HEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23227">
        <property type="entry name" value="HEAT_MROH2B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Maestro/Maestro-like HEAT-repeats" evidence="2">
        <location>
            <begin position="1294"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="1">
        <location>
            <begin position="1294"/>
            <end position="1332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8096421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1563" mass="181592" checksum="EFB84F0BCF863A6F" modified="2022-10-12" version="1">MNQLLESLILQFIRDGDEKQSEQISRQVMQKHPVIYLCKCSEILDEMAEINSEYIRILRLTLQVAGLLIRTEEAKLAVIQCTQVLKRIINRILSQPQISEELNQLLTDIHIKLMDVFDELIINHISDFFESGRVPEIVILKIIQQIFESQSKEYFRQFSYEILQKILSSLSLVKTDQCRLDYCQLLQSIYKTLNHFEENQVQINQEQVQNVSWLIFDQIIKDWMQTTTQVQVLSLVGFMSQFISRERLERSLERLTSVYLSVLKQKSNQLRPLFEGFNKFLERLYHLDRIGYEGIIKSIMVSLYEFYKNPIFSPNYPFDPNALKYKNDLLTLLQTVSKFNIDNCLDLFIGRINSKDSIERVSCLWICNFILSRNFKQISEPYKRQLVLQLLNLQFEQDCEVRYAICEIILQIYYKLKDEELPINQTYLDVKALLFYLFNQVSIHDKELEEYGKQRAAPFETTLELLKSRAQKILTTLSQDNYFYDLLWPMGLEIINNQKFSPGLSHIFKCYTQILQKLNIITNNSPVGQHVIVVRMLLLIQIPYFFPNLGFEAVSFLPHLIKTFDLKVPSSFHQNVETLKQYLINKKHLDEFYEEKVQQIWKVCLSLFIQNKELSQIEEDFQVQLSMYLNSQALRLPIMKMQATLMSFLTNQETIKYNLDKLFSICHQEYTNQALNLNVPIQEPDFNLRLCVAECFGYIGERHTQLVLDKVKSILNCEVIQKKNTGSFKESVLSIFQKSGSDEYLASFRATLLIAYGCLAKQVSLEILEKNILPNVMPFIGHEQLFNSLQISLESISKAIERSIENTDEAAASSFLLSYQKYRDQVLNHCQKNINRGNIFRNLNTINLQYRLAPIEYNKVKQLLSQHLDQKYDLSIEQVNITFQNCFLLFLGTIKLEEDSQTISVWQSLLYLIKSIKDNDQLIEILQKSIQRVKLKMEQNHKNYFKVILTLLSILYRNTNAKRSVVLILEKVMLSLGVYLDIYQKTDEEFNSQLIKSLREKLHHNELTILMQELQNLINEPIVESLHGFVSLIQCIYQSDYADLNKVLFLLQSISQQGTGSSSEELINSNLIKAMELLAQNNLSDSLKILLDNQSEFPMGMFPKLFLNCVSQKKTFMVQSFKFLTDVLNNPEGIITGDIGNYLVNASLFIGQLFIDENKILSEMLPKSMASILGTFLLRFMTCHDPKLSGSSQSVQTAIWAFQNLLTICNIEGISQHVFKKLLIEEEVEDGVQEIVMIYCKGASFQDQQKLFNFIEGFTKKEQASYRLGAIVVINQFIRSYKKEYAQQNVYENLLKTLLESAEDDSDNVRAQVAFGLGGLSYYLKADETIEKDLLQEIIECLINLLDDQQEICVRSAMISLQNLIDSTTDFVVYIPQLIFTTTSNFEKTMTKIRISAFSLFSKLCNTCFVEEIMNEQIMSYLRQALVSVMLHLLDESVQVRQVCKLALEPIGNLLKTPILKQLSNNEMESDLEMSHLEENEKIIMIMSINLEQEHLNIYIKTLIEFCKSPEHNIKGAALFSLIILLAFREVGDYRPQIEQLCKDNSKAKLITKQLVMKAAYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1W7</accession>
    <name>A0A8S1P1W7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1020195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1W7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="97" mass="11365" checksum="998943A7AD53B7E4" modified="2022-10-12" version="1">MMIFALEDTTKNKNDRSSIIKGFVINIKLQICLKIQQDIQNLVYDIYGLGLSMTRSLDNKINSWKGEIERSEISQLIVEVQKIFMFRGDWEYCSILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P2E5</accession>
    <name>A0A8S1P2E5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0990145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8095774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="968" mass="114341" checksum="D78DF836C076D0E3" modified="2022-10-12" version="1">MSVQISSSNDYYGQSIPPQQVTPDQEILEALLSRKPFYESLFNSLVKMGLPVLSITPNQTLIDSINNGLFDIPQLQRLISIKLEGQPISYHRCKLAHSCKNFITECLNSNQQIELEHMLGLIKKTTYPITFAQIKRLDYTQLTYEQEFNYALFLKLLNINHVEPLSSNLTFNTTEISGYYEIKNIQDLRDHEQIETFLAVRKMRHQIYQIIHAIAEILDSLESLNITRIEDAFLKYINIKASLQFPFTKSINQEFIEKYISEDLITDESAQTFNKVSFAQSINDHSKNQDQSTKKINKIPKEDNLLKSKRENKEYTKIKIKNDNLSKFMRKSTKNNMKKSHQELSLKWDQERQSEFEKQIFHLDQINVLNFSELSNQLFNDDISTKPKKPLQFIKRKVHFDVFDSCRYYNGKLIGYKQVNPRQPFHQYSFIEYELDSEEEIAEDVYSEERSVDMTSSDSLQEFIELDQKQSFIQNEEPFTLICDQTNFEQFQNYKAIILSDIIPIFEFDTKINQNAKNLSKKMIKQSKLQKXTFAQIKRLDYTQLTYEQEFNYALFLKLLNINHVEPLSSNLTFNTTEISGYYEIKNIQDLRDHEQIETFLAVRKMRHQIYQIIHAIAEILDSLESLNITRIEDAFLKYINIKASLQFPFTKSINQEFIEKYISEDLITDESAQTFNKVSFAQSINDHSKNQDQSTKKINKIPKEDNLLKSKRENKEYTKIKIKNDNLSKFMRKSTKNNMKKSHQELSLKWDQERQSEFEKQIFHLDQINVLNFSELSNQLFNDDISTKPKKPLQFIKRKVHFDVFDSCRYYNGKLIGYKQVNPRQPFHQYSFIEYELDSEEEIAEDVYSEERSVDMTSSDSLQEFIELDQKQSFIQNEEPFTLICDQTNFEQFQNYKAIILSDIIPIFEFDTKINQNAKNLSKSVHLQNRNDQIIKALENIMTPITNQCLQIQDTEFFIIPQSVEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P4D3</accession>
    <name>A0A8S1P4D3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1050066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P4D3"/>
    <dbReference type="OMA" id="NEFRKCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RING-type" evidence="1">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8097848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="276" mass="32911" checksum="307FAF5AE052308D" modified="2022-10-12" version="1">MMKILIIADKNPVQQFDVNLNQKIYNYMSNLFDKPQEQMIIKLSPTEKQIDYEDEFQKYMSFNTSAIVFYVFDINGNSIQFTKSQLKKQNSIAQDFGYCNQEIDEISLSRNQILNSTKSFNEEKLEMLNSLSTSFQNIDINQDSMTIIQNQKCQFCTDILNETVIETPCQHFFHTKCFSIIVESQLLQKASTLECLCKKQLNFKILFFLDNKYDAEIFKYRYILNQLQVLEKNLQVMYKNEFRKCKNINSCNFFYLHQPFIQNKHLYCPYCLWEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P503</accession>
    <name>A0A8S1P503_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1060030</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P503"/>
    <dbReference type="OMA" id="IMISQPF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001465">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8098061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="360" mass="40851" checksum="D1C38C1E688C822D" modified="2022-10-12" version="1">MNLQISNYKILSILGAGSGHQVLKVQNIQTSQILALKIEKHSKLGQLQGEIEKLKDLVGINGIPQLIDYGKTQDSKSFLITPLLQRNLQEITKVHLLSLHQTLAIGLSLIEILQQIHQNGILHLDIKPENIMISQPFINVSSDQILKPNFIFLIDFGLSQRIELKQYPKKVFVGSLRYASRQAHKGNPLGYKDDLECLLYVLVYLRNQNLPWQSLKQQPSQQMEIKKIGEMKDAIFNTQVLSQKFPPQFSLFKSYIETLTQKTMPDYEYIKNLFKSMLSVGDQSTSAKLTNSASLNQSIKGTKQNQNTEQILMLLENIQNNDSINISEDHIDTETSIVLISDLIKSYPTSSIKSITDIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6D0</accession>
    <name>A0A8S1P6D0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dpy-30 motif protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1070101</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dpy-30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6D0"/>
    <dbReference type="OMA" id="METKHVL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22965">
        <property type="entry name" value="DD_DPY30_SDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007858">
        <property type="entry name" value="Dpy-30_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049629">
        <property type="entry name" value="DPY30_SDC1_DD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36960">
        <property type="entry name" value="SI:DKEY-32E6.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36960:SF1">
        <property type="entry name" value="SI:DKEY-32E6.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05186">
        <property type="entry name" value="Dpy-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8098558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="58242" checksum="E09924F362EF7E40" modified="2022-10-12" version="1">MKQQKKLVLHFDLNKTIILADSKYINQTKEECLQEILVGYAWGKLEQRDEKSPVLWKLLTNNFTPIRPAEDMISYKEYICQQFPLKTEGDPDDITEYNNSAIEQRKQLYFQFVKLGQPCMKLKPEYDRIVKLITLPKAVIEELKQQAEEFGFLNEDEVKQRNLTQLLSDKDMLNNLFSDNKYQLLPTFYKTIINLKKQKREFAIVFRPFGTDPKNILREFNKFCLGEHPCFSGRNNTPIVKFDGSKGTKNYIVLDKQCALVYRQQKQLVTGTLRRTDKQQLEDGYEKELEEEQVQIYNETQMLLKITESLKESCALCYVDDFNFYQAQPSEQNAKQLYVDQQDADTLHIFFDDGIQENENNIVQVTDCVTLENLSRKRCLNKYLVHVDILDVIKDPDYFIKQIEICERNRNEEIERIEKGIPEEQAEIPKKSDWELLEECSDADYLRKTILPLLMPALQLVDIERPKDPLEFIAMYCLKNKEMVKIPQPPEQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P9H7</accession>
    <name>A0A8S1P9H7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1090114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P9H7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8099373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="220" mass="26847" checksum="DF0B6BBD214ED47C" modified="2022-10-12" version="1">MLQFYQILKNKNGRVIVELDEQLMEIIINDYSLHQQIKNETQQTQLDEQKFQRRIVPFFLNQFMHWAKQMDYKKVECYLKVIHNRKTSKQQKWELGDLQKIFGPINSRTRCIQIETQQRWKEFLDQQAEMTVIMNNKIKDQQTKRKYIDAIPQLKQELDKKNPYHRFLSISINPKIDYKISTETCIDEHNQKEFEFFIENSDQEQDRLFTSFIQDDQESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHD7</accession>
    <name>A0A8S1PHD7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1170125</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHD7"/>
    <dbReference type="OMA" id="KPIGYQS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035831129" description="H-type lectin domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="345"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="512"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="350"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8102093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="608" mass="70843" checksum="54EE670205A6F797" modified="2022-10-12" version="1">MKTFIIITLIFICNSLNVNATSELEIGVIQFEEANGSQIYNQLVRFDTPFAKVPQVALALVEHTSTTALFAKVKNITQHDFIIQFFSAVTLNATYRYLATTDNEVYINCSNFRATQQAIFKYPQFQSQVNAIAFLVGYHHNGFVNISINQHSNIQANLSINTNDIQQTAIGMCLIVGSNLQFDDNADTPKFSGRISSQGQIRSLSFRPSIFKILFIEVQQNSGQSKQKIEESLTDLKTFFDQNQNRKEKENEDEQFTDVSEELEPQIIEDLEECQQELNDNIVEEQSISDDFSEEEDDDEYFWNKQDKQQKSSVDFELQLTLLQDALKKKPQIQQQTIKELIKKTSSNQKEQKQKQVIKKQKDEVQKKNKQIDDDQNIKKNDNFKNVNTQNLNNEEIDMKLQQKESHVRSLKKALQSVETAKEKELEVVLPQIELEKQLSQDQELHDIKVDNKQQEILEVSKPIGYQSPLIDDIPKIEEIIEVKQKEKKFTQKLDGYMQSFSQDTYTQKVQKKKQEDQINKNESNQKEIMEQVQLIQLTGQSHSAKVVYEASEDSLRKFEEDFERRKQEKMKEIKQQLIPENKPTKVKFLSLAQEDEQLRSYKDFFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLW6</accession>
    <name>A0A8S1PLW6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1230051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLW6"/>
    <dbReference type="OMA" id="YEDENAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="255"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="296"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8104197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="576" mass="67606" checksum="DE2E4AA9E936E61E" modified="2022-10-12" version="1">MNSSSSSQLLQKLQNLYSSNKKQSEHSISPLQPRPFVCREFNQLIEKSSPVPYLQVKQHDFEQWTERQIKQIQKASCSSFTQNENVFDIHLQKYSQISTISQQTFPVLGQIEKKIKLLKYAKENDHNTFAAIIAKMKLFCLDNAQTCYDFYRSTDLILLKYMIFLLIQELNQRPTIEKQEISNDQTSQFQSMIKDIISIVYEIIEEVKSKSFSGSQIDTLNKQLAKIQNKFKLDSNSTHKQINKSHHQRISSCNFETCRTPNSQNHSNKESNISSDKFKSAQKQQQNNVSGSQKIIQFQIEELEQKKKLISKLNEQITKLQNTNQQQQAQIQDLNDQIISIKQTLKQKQEEIEQLSQSLESLNQNNIIHQEEKEQYNNLFHEYEDENAQLKSNLEQFQNTIELCQQQFENQKQETFAYYQNQCNQIQQIKENEINHLRNELHENQVIINHLLSDALYFGNQYKNLVDKIDNLKPQSIVNDLNQIQKELFYNENILNAKLNAISNFSDNLLFDAGQEYLSQSLSQHKIMNSQELTNIQQQNNSKKSNSNNYIIAQKNQFEIMEMLLIQSQVLEKFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PM61</accession>
    <name>A0A8S1PM61_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1210133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PM61"/>
    <dbReference type="OMA" id="FYQINES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="203" mass="24145" checksum="EB496ED626F4741D" modified="2022-10-12" version="1">MFKIFRYVFIAYQTYKAISNQKTFQINSLFIFLSYSLMGMCNSQKAKPDNNDIPINQKQKLEEPQSNTKPLLEQYHKLNALLDLLGNLDLLEKQTQDHINLLYMIRTNIKILIQYNLKKIMKEKNITRISTIEDEEWLYTDSLFLSRIIPQYQQLNIVLETLFQNDDFQNAFPILIHGFYQINESIKKVPIQYTFTKTRNSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PPC8</accession>
    <name>A0A8S1PPC8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1240087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PPC8"/>
    <dbReference type="OMA" id="NDWEIET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014807">
        <property type="entry name" value="Coa1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08695">
        <property type="entry name" value="Coa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="353" mass="40551" checksum="F980D9AAE6ABE6E3" modified="2022-10-12" version="1">MFNKLKNSFPFRSGPNFLLCFLGFSGVGYFGTSYLHKQKYDHPIVQEALRILQHNDQIIQLAGYPLSLQINMGSNATQNNDVCQFSFQVVGPKGSAKVELMGQSRELQEIQNKTQYYIPSPKQMIDVMKYNKDIEALQNWKLTPDVKLWKIDHLVADIGLQGDFRIVLVDSNQVLKKDVAQIMIPPLVSDRRTLYDLKLEIDARKPKTPTTEQEIEEARRYRQQELYRKVGGVRNITIISTIIGAMAIYNYVKANRRISVLNTQIHRQSLIIIQSNPQIKKIFGQNIQFNQQLRGGQIRDQAEFETDMYGQKAGVCYIKGQKNKKTNDWEIETIDVAIKDAQGIINQKIRILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQ02</accession>
    <name>A0A8S1PQ02_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1260066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQ02"/>
    <dbReference type="OMA" id="KGAICCR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="338" mass="39375" checksum="22C9AB17A6DC5569" modified="2022-10-12" version="1">MTDLFVNFPQIYCSKHPCYQLNMVCRHPNCNNKGAICCRCQLKYHCDNGHTLESFVPLDEVLILCQTQNPKAEYFEDLSVDGILEVQQLQSTLSNINNSIQEQLKRELLILGQYQKPFKDIYGIITHYSQIDGDISRTDDGTQGGMKSDFLNIMQYIANKIIIVDGEFTIDQSDMNNCLTEIIERKKKNLNILQKLNKKSQKQAKIISALFQTGCSFGLDQHIPISQFDSWVQIYNAQYFENTSYQDIVSLVIPQNSLIMVGILKEKYLEFCAIDRYKAIFKGKINSYSKLKWTLNQVIFGCECLKWNLNQQLLDQQIDQKEYLYHLNDRRVIYYLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTD3</accession>
    <name>A0A8S1PTD3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein transport protein Sec61 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1310090</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC61-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTD3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030671">
        <property type="entry name" value="Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13509">
        <property type="entry name" value="SEC61 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8106640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="8820" checksum="A309942DBE60EA5F" modified="2022-10-12" version="1">MSEKKDLIKEALKRRQGGGKSPSKSSETGGQSGLNFYSGDVSSLKVQPNTVLIISLVYLGIVVLLHIFSKLRSGGTETPQEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PVY1</accession>
    <name>A0A8S1PVY1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CP-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1330152</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PVY1"/>
    <dbReference type="OMA" id="FKLDGLW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04178">
        <property type="entry name" value="Nucleostemin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1580.10:FF:000002">
        <property type="entry name" value="Guanine nucleotide-binding protein-like 3 (nucleolar)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:005957">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014813">
        <property type="entry name" value="Gnl3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08701">
        <property type="entry name" value="GN3L_Grn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="6">
        <location>
            <begin position="132"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8107405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="426" mass="48291" checksum="041DC26EE498D593" modified="2022-10-12" version="1">MAKFTNKPKGRTSKRLALNKKYKIEKKVKQHHKKLKKEARKMSALGQIKKTSSKEIGIPNMYPFKKNVIETLKRKKDQEEQEKKILKLQQKEEIDLNLAEVKSNLYETKAQVIVEDEQVQQENLTQLTKEHKKYITQVKKVVEAADIILIILDARDPMACRCKHMEREILGMPGDKKIILVLNKIDLVPPGNADAWLTQLRREFATVLFKANTQQQQSNLSSASIYKKTLSQRQDLADDLTSSSKAIGADKLLELIKNYSKNDGVKSSVTVGVIGYPNVGKSSVINSLKRSKACAVSSTPGFTKGLQEVIIDSQVKIIDCPGVVFDSENKESTLLRNIIKIEQIEDPRQPIEEILKKVSKNELLLLYKIQTFNNVNEFLCQVALARGKLQKGGIPDLECAARIVLQDWNQGKIKYFTVPPNQIEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZR6</accession>
    <name>A0A8S1PZR6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1360028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000139">
        <property type="protein sequence ID" value="CAD8108139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZR6"/>
    <dbReference type="OMA" id="VKQIYLF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09212">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018997">
        <property type="entry name" value="PUB_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09409">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUB" evidence="2">
        <location>
            <begin position="391"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8108139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="476" mass="56281" checksum="C2523F5EE195309A" modified="2022-10-12" version="1">MGVCQTTKMKDGQQNKKIDISQNASQVILDQYPMPKPKLGIGLPCMIKFFDQIKIIEVTESMAYNFIVRLGVNQQKQVSKDQDYQCVYLYSEKANIDFFECGFTPIDVSRPDKIIEDKKQYKFLFDFKNLWVFRDYGEEVEQVLNIFKEINVKVKQIYLFTKPLNHFIDHYQMLGQISILNSYYPLLLFDYKDVSDATKRGDDTFRIYLHSQEAFELLIQQNKISLYNRNLQIRNLIFLYQIVNFSEKLEENQYKVACYNLTQQPSGQINKQKLIKELIPQLQNLQQMGSNVVLIYSSKEPQGIKTARLAIEQYLARGLLIYPEDIKKYNEQLKSLQITPEQLIQQQKQQDNNKINQQQQEIIQLSREQEIINIYESWFKKIKSQILLQSSHNLLLKLITNITEYPNEEKFRTIQKSNKTMNLNILQYEEGQQILQLIGFREGETDYKNIFEIGQLKLARADIEIAWKKQLEKSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q0L6</accession>
    <name>A0A8S1Q0L6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA (guanine(37)-N1)-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA methyltransferase 5 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T1380062</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="9">Predominantly in the mitochondria and in the nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRM5 / TYW2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="9"/>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q0L6"/>
    <dbReference type="OMA" id="VGSHSQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070901">
        <property type="term" value="P:mitochondrial tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03152">
        <property type="entry name" value="TRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025792">
        <property type="entry name" value="tRNA_Gua_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF43">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="11">
        <location>
            <begin position="109"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="389"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="242"/>
            <end position="243"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="270"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8108795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="421" mass="49147" checksum="522650C5EC9E7570" modified="2022-10-12" version="1">MKLTQEIKLSLQKTQKVISIQMDVHHINTFVKIFKKFIFPGSRWQQEQQNNKSINTTFQEPFPENLQEFIKENNCQILQKEVIVGYEDMTYNEVLQKLLPQNVSAPQGYEIIGKIAHFNLSPEQLPYRYLIGQVLLDKNKHLQTVCNKLEKLHNVYRTPQLELLAGKNSYDAIVPEGGIRLFLNFEKVYWCTRLYSERERIIKFIKELSNGQKIKVLDLFCGIGPFSIRIAKDLNANCLANDLNPECFNYLQKNIVENKVQNQVIPLNMDAREVVLKIYDKEIDFDFNHVYMNLPVLALNFLDVFKGFTQKTGKQDLPYIHVYGFAKGKDDQELIEQFSQRIIKGLPGFDKSQILRFHILKNVTKMKKMCCLSFQLDKKSAESDFGLLEQDDGNNSDFEDDEKVEQNEQVEEVINKKVKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q430</accession>
    <name>A0A8S1Q430_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1440029</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q430"/>
    <dbReference type="OMA" id="RIRVWIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040132">
        <property type="entry name" value="Tex1/THOC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3 THO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="195"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8110399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="323" mass="37067" checksum="FC311FC4A62999AB" modified="2022-10-12" version="1">MFYQQFKEYPKNSQREKTQQERKTIKSMDWNCNGSYLATSSDSTIKIWSFDGNNIKRSTDLKQGSEQLEKLLFHPTQPDIIASITKEYVKIWDIRTKQSVRQEKKEQMGIACWSPNGNELAVGTKQNKETQIMFLDMQGGERQIMYQPKDKDKNFELKNIMYDKDVFVAVGQQNGVGMIQFMDVNNPQLPPLHQMEAHRASITQCKFSKDGQHMYTGAQDSLIILWQLPDLLCAGQLMTVESEIKSISVFDSKYIAAIGLEEQAYIYQVDRCLAGKTDHIDSIKLEGQPSEICFHPNKFILAIVCQDKNKQESVIHLYGNLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q461</accession>
    <name>A0A8S1Q461_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="13">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T1400158</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPP phosphatase family. BSU subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="13"/>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q461"/>
    <dbReference type="OMA" id="MVPVNDH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000203">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000204">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000008">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012391">
        <property type="entry name" value="Ser/Thr_prot_Pase_BSU1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46422">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE BSL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46422:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036363">
        <property type="entry name" value="PPP_BSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="15">
        <location>
            <begin position="559"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="342"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="770"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="342"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="374"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8109468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="791" mass="88823" checksum="F32C1B6F23ECC36B" modified="2022-10-12" version="1">MNNFEKVDAFGQLPQARFGHTITYIAKGKAILFGGATGDTGKYSITGDTFSFDMQSKQWKRVEVQGTAPSPRAAHSAVCVDINQIVIYGGATGGGSLASDDLYLLDLRSNDDLGQWTVVPVVGTTPGRRYGHTLTFTKPFLIVFGGNTGQEPVNDCWCVNVEKSPITWARLECKSEQPLARVYHSASICTNGSANGMVVAFGGRSNDQLALNDAWGLRRHRDGRWDWVKAPYKIDKEQPVGRYQHTTMFVYSMLVVIGGRTGNVGETLTIDVYDTETSEWSKFNSIQRFRHSSWLVDTNIFVYGGFELDSPNIPTDIISKINLNRLLMPSETLTNKLAQYQHAQRNVSPQSLSPQMTPISPDTQASPLNNSLMQKKAGQTNQNTQKQQKPFAQNQADKSQFKFINQAVVAEETRDSKAPNRKARVPQTAQENNIAHLFISTLLQPKTFINISENAKFLFQAQHILMLCDQAEAILKEQPMVLRCKAPIKIFGDIHGQYSDLMRFFDLWGSPFVDGKDSDIEAFDYLFLGDFVDRGNHSLETICLLLALKVRFPESIHLIRGNHEDKWINNGFGFSEECAQRLGEDPNDDDSVFARVNRLFEWLPLAAIVEDKIICLHGGIGSQLNYVAEIENLQRPLEVIHEVTTPEQQLVVDILWSDPTDTDQDFGIQPNIIRDPAGTGNIVKFGPDRVINFLIKNNLSLIIRAHECVMDGFERFAGGQLLTVFSATDYCGRHKNAGAVLILKRNLEIVPKLIYPQNLNAHNWIEDEEALKKRPPTPPRWKNQGQRRSYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9C5</accession>
    <name>A0A8S1Q9C5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1470050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9C5"/>
    <dbReference type="OMA" id="HVERTNM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="179"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="95"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8111311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="249" mass="29288" checksum="573E17D0BEA0AAE9" modified="2022-10-12" version="1">MLKQLEDALIKLEETSNNCFKVLEKERQNRIKITTEIEQTQQELEEILNNEEQMIRNIIAVELEKPVQIGTKFQAKLIKQIEVAKFKHVERTNMVDELLETNTNLRIELHQLEKQLQRSQAQVQDFGNQLSQKQTEFLDLQILQAAKHKELKELIEEVQEMKELLQKVKEVQYTLNKVIGKNNNNNNNNNNNNNNEFSKTNSTEEAFKLTQSKRSVNGNSLSNDYQQDKNEEFWYTLPPTPPLKLPRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9M4</accession>
    <name>A0A8S1Q9M4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxypeptidase</fullName>
            <ecNumber evidence="6">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1480109</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9M4"/>
    <dbReference type="OMA" id="GIDCYNV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000408">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF3">
        <property type="entry name" value="RETINOID-INDUCIBLE SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035922685" description="Carboxypeptidase" evidence="7">
        <location>
            <begin position="16"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8111684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="469" mass="52954" checksum="FD4A67605FBDBB6F" modified="2022-10-12" version="1">MQKFILLTFITLGLCRLSFKEGLYNYNDIFKSEGTFPQGTKCKKVDAKNLDPLFDDTQVIYSGYLSVYENSNSALGFIFYGSQKAKDESELQNYPTLIWLNGGPGSSSQLGNFMELGPLLMQSNGKLKKNLYAWNTEYNVIFVDQPIGTGLAYVEKQSEVPTNQKQIGQQFLYALNQFLFGAKGCVEQQQVRGLTQSPWFVFGESYAGKYVPTIAKAILDYNLNAQKKIPLKGVGIGDPFTDPYAVIAEYAAYSFNLGLIDIQERMQVDSILAYGMTELNKGNTLAARDAFEESLDIIITQSGGMNVYNVLQYGNYGEIEEKVEDYLNNIQIVNKFGFSDDWKYKISNADDGPVQKALKYDFMLRDVVVTVEEVVRQIPFLIYNGQNDLICSTPGTLRWVYDLKYEKIEEYRQKDFEVLKITGTERIVGYQKSAGNLELVLINNAGHLVPTDQPEASLQMVTTFVNKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBQ9</accession>
    <name>A0A8S1QBQ9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1540093</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBQ9"/>
    <dbReference type="OMA" id="EPTQFIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="84"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8113209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="377" mass="43714" checksum="65BCC4BDE8727B3F" modified="2022-10-12" version="1">MMRLQYLQQPNLVIQFNNYKNPKYCLELKLSTQILWKMGKQNLKAFGIFYQNKIKYFEAQHVFLEQFKKLIGGRVIYRNMASFYEPTQFIGEGLSAKVYRSIEKQSQKAVAVKMIKQEFGKEEQALDIVKTEVQILQSLNHPNIIKVLEVYENDQTFWIVQDFVQGTPLSDILKQKLPTEQIKKIMSSLLNTISYLQSLRIVHRDIKPENIIVEKDGSIKVIDFGFAANLKFGSVSSVCGTPGYYAPEVLRQKESSFNSDMFSVGVVLFNLITNQPMLKSKMYNAQQYVEDEEAADLLKQMLEVDPVMRITALQALQHPYFKGFQQNNEKHKSSEHQILQQQLVINQQQQYQPLKKSNQSLDAKTIKSLRTTNIMDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QC03</accession>
    <name>A0A8S1QC03_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1490096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QC03"/>
    <dbReference type="OMA" id="FRKKNCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="75" mass="8735" checksum="99EAC1730F30D140" modified="2022-10-12" version="1">MSEEKSLASASKTITKKPCSWKQMFRKKNSEQQKKKEELRKLGEEIAKTEEFLQNAWAIVAKIINNKSHLNQKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF49</accession>
    <name>A0A8S1QF49_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1590014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000164">
        <property type="protein sequence ID" value="CAD8114242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF49"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="127" mass="15347" checksum="742F84D1ECA84F1F" modified="2022-10-12" version="1">MRFNYIVIENKQNIFFWIGFNFINFGQDQSFSRLEINNLIMINFMQDQKLSSSLSILIDQEIFEINFLIQGRTNDEIQQQIQNIIAAQQFLYKYCNWKIDYIELLENNLQKALEQFFLLMILIQDTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QH42</accession>
    <name>A0A8S1QH42_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1620035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8114953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QH42"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="8125" checksum="BFDAABB10AAD4293" modified="2022-10-12" version="1">MCKNIMNQKQYKNDMKNGEGLFKWPDGCRFQSNWVDGKLQGESKLIENGIMTIGEWLNNKCVKWEQLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QHE0</accession>
    <name>A0A8S1QHE0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1620075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8115033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QHE0"/>
    <dbReference type="OMA" id="MNDSETC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="444"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="490"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8115033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="665" mass="78680" checksum="62E900F6EA8B2CF9" modified="2022-10-12" version="1">MELENSLLCKHNFQALKIINDSNIITTQRLLCHQCLKEHQNKSQLSQLMDISVAIAQVNKSQNYTSKYTNQLIYQSITHLNAFIIKFKLLQSEIQEACQQINLKALQWLQDLQLQQNKLFIERFDQSFNNQQSINDNDLNDVNYKFLSITAQYLPDLNSLINPFISFQQFNSCQQILLQLSKVNIEDAIQQLYVENRCKTLIEKREIMQQNSICDQEFINIQDMIVHFQEQNKGIQLEIIEQLQSLIGLRQKLSQSIHNLEIELNHNIFQQYGKYFQIVQNQNLLINQGCMDKFNEILNKTQKDQVIIMREEFKSKLTNCVQDLLTNHLKDLQKLIVQEQNLYNFISLKQIKSQIKQNQYCYAIQFNKDTSIMISTSSKQIKVWEFNKGQLILRNSLQKHIDDVTCLLYSNYLNSFISGCGNDDGSIVCWQMGNNYEWKSSQPFQQHKLGLRCMIMNNEENQLITGSLDKTIIIWKLDFKQNNLVYQYTLQKHQNKLLSLSMNDSETCFVSCSEDQSIIIWIKQQNIWKFKQFLQQSICDIGRQIKFLSDTQFIWLQQNQGILHFFEGQNFQFEEITEKQLILNNQKQDWNLFPIIYNKLKNIIVIRYNSNVFILRIQQDGYLKIMADPIQCQSEFIYGSLSKDGQYIVLWDMVSKQYNIYEILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QII0</accession>
    <name>A0A8S1QII0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1610107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QII0"/>
    <dbReference type="OMA" id="MYDEYLD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="209" mass="24468" checksum="DCD63EC6E4BBC76B" modified="2022-10-12" version="1">MTFQLPHNFQNRSVQKIVQELMYDEYLDRDNLLRNAFISSPLKSGKKKISISKHRYNHFLQQSPSQLYSPGLLNNQNKVVGIIAMQKKKNSVPLNVSVNQLIENNTSPTNNAIVRVSKRNKDYKPKELRGLSISDHVSRQKLVFPQVQRLSQVKQQQFASPNFNFIDENFEGYKLKSEEMSLEIDEYMRMQKHNNNNLNLLPNIYSRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QME0</accession>
    <name>A0A8S1QME0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1700035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000179">
        <property type="protein sequence ID" value="CAD8116241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QME0"/>
    <dbReference type="OMA" id="QYEPYIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008833">
        <property type="entry name" value="Surf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34348">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34348:SF1">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05477">
        <property type="entry name" value="SURF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="232" mass="27527" checksum="C58EA7FBDE00C497" modified="2022-10-12" version="1">MNKNKESGKQAQNNKQNDSNLNKNKELQKLVQNNKHFEFLSSGKIKCILTHHEILPTLQEFNNYLNGRSYKNALENDIDFTQFEPYIVQHKTDKNKLYCNLTRQNISKKKSVVLKHVNGKRYKYYLSKYLEEQAKEEQENQPENQEEDEILNELEELNNLLNDEVEEEQTDKQKNGILKGGKKINKKKVKPQQEQQIEIEQTIQTKQKKQQQQQDGKQIKKLKNKQQKAQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNT8</accession>
    <name>A0A8S1QNT8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2010003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000210">
        <property type="protein sequence ID" value="CAD8117488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNT8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="11796" checksum="9FA86EBBA197DC91" modified="2022-10-12" version="1">MSLNLSIQHNHFFINYAFFQLAKLNGLMQSRFFDPKNQFDTHIEIKEINYSQNQMLLEEITIAQSIGSITIILLVIYRFGNVQDITILSRTMVFLDYNQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPQ2</accession>
    <name>A0A8S1QPQ2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1950007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000204">
        <property type="protein sequence ID" value="CAD8117311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPQ2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="119" mass="14418" checksum="79AC5A5FADCF83FB" modified="2022-10-12" version="1">MDKNGINVSHPQYQEELPKLFVHIQSIEKECDDLINRMNIQMDLLQQEFYLLIGGIISKYQISKQQFLNLNSQQMNSYLEQSVHFRSFEFKINQLLQQFKNEFQFQIQKLLYELSLYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QR74</accession>
    <name>A0A8S1QR74_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1880006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000197">
        <property type="protein sequence ID" value="CAD8117097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QR74"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="67" mass="8314" checksum="43C9CF6645B3B51D" modified="2022-10-12" version="1">MQNKLYLNIITENTNIFESSYTYLEKLIKILEGTTLKNWNDQRNYYLMKLQKVSDWWAKEIKFFNQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QS64</accession>
    <name>A0A8S1QS64_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T2380004</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000247">
        <property type="protein sequence ID" value="CAD8118413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QS64"/>
    <dbReference type="OMA" id="FRITQII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="13"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="115"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="149"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="183"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="217"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8118413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="37601" checksum="4F9A1061CEC889C0" modified="2022-10-12" version="1">MYGKLFSMFIQQFQKVLNKAAALQKLKRFDEALQFYNQAIDENPEVSTYFFNKAVTLQEMNEDEEALKFYDYAIQRDSMDSRYYINKGEVLQKQNKFEQALEQYDYAIKYNPENSKYFFYKANILSKMNRQEEALTFYDLAIKLNPQDSENYVGKANTLNDMNKFDEALKQYDYAININPNDSRYYGNKANTLVKLNRFTEALEYYEQAIKNNPENPIYFFNKAVTLDQMNRFEEALEYFDYAIQRNQNDPSYQTCKGISKKQIQIFQQKFQKKQKNCKKPNNRRIMQFRITQIIQTINLFKQQKINNFFSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JLQ9</accession>
    <name>A0A8S1JLQ9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0040049</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JLQ9"/>
    <dbReference type="OMA" id="WWKQFWL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002483">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF139">
        <property type="entry name" value="PROTEIN SCARLET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="571"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="28"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8042834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="597" mass="68003" checksum="6DBE18424DB8A12A" modified="2022-10-12" version="1">MTLLQVLKVLEQKRQWIQHSKIQIIQYLVLKDVITLFQFKEQRQILKNLDGFCKGGEVTAILGASGAGKTSLLNILSARISNTKTVKLTGKVLVNNIEYNSETFSNFAAYVMQNDVLFETLSPREAFEFVANLKYTDPELKISRVEDTIKTMKLERCQNAIIGGPNLKGISGGEKKRTSIGFELVTNPSCILLDEPTSGLDSFTAFQIIHELTLLAHEQDRTIVFTIHQPSSDIYLLFDRIMLLVQGKFIYQGPRSNLINYFKGIGFSCPDHSNPLDYMISIMHQESQINVDNYITYFKEYDNQWAPKVQKEIESSSKSEIQFKKVETSTLYQINLIAKRALKSYFRDKLLIRQRFGMALFMGLLIGGSYYGIGSSQGNYMDYSSMAGLLFFLCMNMTFSSLFPVVLQFATERDVFLREENSKLYTTFSYFMGKSFVEIPFCLITPIIQELILYWMVGLNHMNSGIVITHIFIAVLTCLNGNSIGLMAGCAFNDIKAATGFVPLALLPLFAFSGFYANPKQFYDWIGWIQYVSPIKYSFEAIARNEFQSRSYDFGDPNDNLGFNVGQWESVGILIAFFLVIRTFAFMFLHSLRSKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JLW6</accession>
    <name>A0A8S1JLW6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JLW6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="260" mass="30136" checksum="00D6E8114707290F" modified="2022-10-12" version="1">MSSCQCLDCGGRINKKILISKKELNDPIQSNSKLRFPISPQLSIQPQYVRPSNRDLSLVGSPSHFSIILFLFLCEKHSKQIIQKSNRVSVMPVIQLIKAQNLMKKVSETHKIEKEIKTKMSISTHNLFNKVQQKMIINNNHNTDKLKYIQVIENKNSKEITNKLHKYTSNCGQFRIKTESYNYNFASNNQKISVINIPNLTHLENLNKNSISIYESKTPRLFLARSETYNLKQPLTQRLMCTYLKKLNINSKLIKNNNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMA3</accession>
    <name>A0A8S1JMA3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMA3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="24920" checksum="260A1B956FFB0800" modified="2022-10-12" version="1">MANSQEKMQQDYIWIRDQSTGDADVKMRTFGQHYLYYHAPNKRERLEMIWRSMGKAYDWEMEKFRMQKKFIDRGNKRRFFKNFFRFIKNPFGYIYWKTYRIRQPKGRIITTMLGLGVIGTLYKYKMESNQIQKREYYLLTAGKNSEGSGLINTGYNNDKLARQGMPLTQMFYSYLLAKDIVVSRSRDQNYRKYFEMRKKYQIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNG7</accession>
    <name>A0A8S1JNG7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="162" mass="19477" checksum="F80E423280CF5857" modified="2022-10-12" version="1" fragment="single">MNNTQITDSSLLTLKREMFRNKIRRELLEKKFKYKRAMKMKVVFDQIGKIQEIKNNNKDSDQDQYQISEESNDYFAVFQIGRGSKRQILTYKILKSMKFYLQKNTNYQTQMFKVFHFCEEQLLNSKLENTEYIPLLAEIIFLLQNYSNKIEPQPIVVLLRAX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNP4</accession>
    <name>A0A8S1JNP4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNP4"/>
    <dbReference type="OMA" id="FNETRFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="175" mass="20917" checksum="7BA4586A84C08D46" modified="2022-10-12" version="1">MCENYCRMQLLYQLKQKKSIESNQPSVLYQKISYIVKKAKQQAQKSLGETQIQAVFNETRFQSSNRQQFYKQNKLKEIKYQISHLKTEQKSINFDFHSKVSRRYAKSITVQRDEPQNEQQYYQSPQNYRYAQFNNIPKKVVIFSQRATPQRKNINIGLNLDTLGSWRRNSQDSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSM4</accession>
    <name>A0A8S1JSM4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSM4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="74" mass="9185" checksum="BE96EEA4ABF24D90" modified="2022-10-12" version="1">MKKELENQKYQIQQFHNSYEQLTYNCYEFKDEIYKQNLIIQSQNNTIKEQEALLNKLENNQQRNNMQFSQMMEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTD1</accession>
    <name>A0A8S1JTD1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTD1"/>
    <dbReference type="OMA" id="REYFTTE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="170" mass="20090" checksum="2F026499C1BE165A" modified="2022-10-12" version="1">MKKNTRKKQTLEQQEEQQRKKTKLEILDADQHISKYNNNLDHLIDIFTRYEREDDFTQNLMTTSKLSKIQKIHNLSLDTFKDESFSLGPKVENGDFLQGDKVHKIVSVKKSYLNGKLIATIQWRPRSDGTIPKLREYFTTEVAKYAPKELIKYYKSRMVTTTIIEQQQDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZ31</accession>
    <name>A0A8S1JZ31_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZ31"/>
    <dbReference type="OMA" id="LCATREK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="57930" checksum="C4EC621A0C5BA82C" modified="2022-10-12" version="1">MRRYSENTESSKSQISIKKKGLQIAYQSWLIILGIILLTLSILSYSSFQYTIDNYEYIILNWHQQPLVQIKITNGSCSQEEEPLIEYKWPGTIDGCDCSTKRRLLQEENIQSQKLNQLIVGKQCNQTQLRSGCSTISSINEKQFILFPSSNNKTGFQLCATREKDNNFYKWAPKSKDCRDGFLKCGENDDQFYCTQEKVCPIRRIGLKSKNILENYEEGNTLDQDTIIYSRISNEYLPVAEIRIGQGGVCLRNNEYGITNGREDYPLMRIKRKECQYDPRFEEVALTTEDIFYNINGLSNLSEVLNGYEISNQTKWGLYQRSYIPWKMKCRGQELNEFLNQQIYLNEILDGQTSQLIISVFFFVIISIVLSIFTFMNIMGKQIPCLQTKDQDETSKRLFLIEIGVKFVIYVPFAILISLEFSKIQKELDFFDQVINLDCSDIYVKNQLNSMRENLMFGVYQINSAQFYLFFITVLIDILFIGYTCYSNRQYKNIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZK9</accession>
    <name>A0A8S1JZK9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0120213</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZK9"/>
    <dbReference type="OMA" id="AFGIWEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="7"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8047902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="387" mass="46110" checksum="A49BC9C76951BA2F" modified="2022-10-12" version="1">MNVINNYTIHKEIGKGATGKVYHVTDEKNYQYALKVQHNLTESEKKMNEQIKEIKFQNVINTYDQFQYQSIIQCFCIIMDFCNLGDLYEYLNKYRLILNIQQKQNMLFQIAFGIWEIHQLNIIHRDIKPQNILLQCFDQRNLQLKICDLGLSKLQENQNLNTVNVGTPYYMAPELIDFTQKKGIYDKSVDIWAFGALIYDFFSNYQLFYGLQIYQIFDQIKQAKDLDKKLREKINDPLLLNIAISCLSYEPSKRPDIEQILLELNQPLFQYQQKIFNEQNQRWLQVQDQSQEQYKNQQKIEYQKANQNNSQIQNLDLSQSSLINQTQKEKSKNHIKNQVRLRIFLSDQALIEQLCIMIEEGNQKDEILKHMNESLRNKLQAKLYQTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1H5</accession>
    <name>A0A8S1K1H5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1H5"/>
    <dbReference type="OMA" id="EDCLYNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="61" mass="7200" checksum="4348C1EE0F49B480" modified="2022-10-12" version="1">MNIPSEFKSVTDYAVICGKRCQLFENFLRGRRLKNFEDCLYNCAEHVKDNILKLQIDKSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1K5</accession>
    <name>A0A8S1K1K5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1K5"/>
    <dbReference type="OMA" id="FKLCMAM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="298"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="610" mass="73522" checksum="4485268C36DB769D" modified="2022-10-12" version="1">MNTKITLRNSKQNSFLSSFKSTHRHTLSQGIELKMESPTNRETSGFTSFQSFKSSSMHLKKLVKSPNLSLKSARLYDLNDNFLNKRTSIVQMMQTFSNNYDIIQQSEPLENIDLQKLFPVEEQKKLQNNEIVSDISKLRIFELKQENNDWIQINRFRFHYFSIKIQGQESPIQVYVKCDQSRLLNYRMYISTTASFPTKFNCEAVIQSKYFKYQDKKHNDKFLDKFLYIAIYSEEDCEISISFIYGNKLIKKQKPKREKPNYDKYKYWWKQSKFDMSYETDKINSNLDTRQYSREARKEKLIQSQDQLDQRLKNAITKKKELCEDKKMDLLSKYQANEALKEIRNQQKLKQRIRQALIKFQISWFDVFLLIHLAENMKVMLDEAKKRSKYLAKGRLIVWKYRTTTMIKIKESGQTVDQRNLFKLCMAMCMYTFHNKRRIKTQVSKVLRYFFTLTHQYCHFIQKHQELTNKVNYVKRVFRNLKYKEKAYKDRLWRILNKYAQEILHSLGAHYSNPKKRTYIIDSSALMKATEDFLISKKRKCQQFIQSYVREKDEKQKVIDLALNMMQPEMFDTPSYDDLYGIFRTYIHYKKMTSQHSQQQQIKSQQLQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2D3</accession>
    <name>A0A8S1K2D3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial</fullName>
            <ecNumber evidence="20">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PPRIM_AZ9-3.1.T0100326</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="15 20">Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 20">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="18">Flavinylated by SdhE, about 5% flavinylation occurs in the absence of SdhE.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 20">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Mitochondrion inner membrane</location>
            <topology evidence="1 20">Peripheral membrane protein</topology>
            <orientation evidence="1 20">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="20"/>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2D3"/>
    <dbReference type="OMA" id="MWRCLRV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000482">
        <property type="entry name" value="Succinate dehydrogenase complex, subunit A, flavoprotein (Fp)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 20" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 18 20" id="KW-0274">FAD</keyword>
    <keyword evidence="6 18 20" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 20" id="KW-0813">Transport</keyword>
    <keyword evidence="5 20" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="21">
        <location>
            <begin position="50"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="22">
        <location>
            <begin position="500"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="55"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="444"/>
            <end position="445"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Tele-8alpha-FAD histidine" evidence="19">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CAD8046386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="636" mass="69876" checksum="033506A0A59A27BD" modified="2022-10-12" version="1">MFAITKQALFRQQAMKLMNRFAVVANNKPEKGASQGHLTDKYTVIDHTYDAVVVGAGGAGLRAAFGLVELGFKTACISKLFPTRSHTVAAQGGINAALGNMTEDDWRWHAYDTIKGSDWLGDQDAITYMCKEAPKAVYELESYGLPFSRTPEGKIYQRAFGGQSLKFGTGGQAYRCCAVADRTGHAMLHTLFGRALGYDCIFFVEYFALDLMMDDQGACRGVVCMSMADGSIHRIRAGYTVIATGGYGRAFQSCTSAHTCTGDGGGMTIRAGLPMEDLEFVQFHPTGIYGSGCLMTEGCRGEGGILRNSLGERFMERYAPTAKDLASRDVVSRAMTKEILEGRGVGPEKDHIYLHLNHLPAELLHERLPGISEAAKIFAGVDVTKEPAPVLPTVHYNMGGIPTNYKTEVLNQVNGKDQIVPGLLAAGEAACASVHGANRLGANSLLDIVVFGRQAANLVGEKWKPGQKQPDLPKNAGEAAIARIDRLRHHEGSQTIAQVRKDLQRTMQKHAAVFRIEKTLQEGVEKVKEIYTRKDDVRIKDKGLVWNSDLIEGLELENLLLQGKMTIEGALNRKESRGAHARDDFPDRDDKNWMKHTLARIQDTKSGDVQLTYRDVITKTMDPNEFDTVPPKKRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K301</accession>
    <name>A0A8S1K301_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0130204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K301"/>
    <dbReference type="OMA" id="CKTRNNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="93"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="127" mass="14644" checksum="C695E390401A3186" modified="2022-10-12" version="1">MNQNNVGLMNRKYQCINTDQRISLLVDILVEGLSIKEAAKKNNIKFSSAKVILKLFKHEGRIGKLKENQLSQVDVSSQIKQQTVVLDDNTLLCQSLLKQIEDIKTRNNQLQQTLTIYKQINNQFKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K842</accession>
    <name>A0A8S1K842_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0170294</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K842"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="211" mass="23996" checksum="219EBAC272BA86D1" modified="2022-10-12" version="1">MKVDFLTRFKGRTSTMNKREGVTGIIQSNGTPIEKMPASYTFSSYISNQHVYNYSDIEKQSQQSNDVTPTSKDPQIITNIKTFNKNRKLSHASPLKPGTNMSNMYRSQFVPDKEMYSFPLSKTGSVNLNEYELIAIPKSVLQFGIRVEHTDGDQPKQTILPNISRQYYSKSQSLDLQQDRPMVKSEILNQGSYAKQKEKRSLLIAGLNSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9Y6</accession>
    <name>A0A8S1K9Y6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9Y6"/>
    <dbReference type="OMA" id="DCHANII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="492" mass="56599" checksum="5A4CEE6340DEF7E1" modified="2022-10-12" version="1">MFKPKIQKEQQQQPFYLSWFANQGENPKRGGTAPNQSRKDKLSLKSPYQQPLITVSHQAPNPYTKTIQPPKSYQNNIQQISKIYAGSFQGMMKRRSLEKRYDDIPKSGKTSFKPSRTFTQQNSRAFHSCEQQSRPQTQQLNTFEPKIQCDNIVSYNYEDPQLTPRDNDDVEIMEVDNVDINSNESNEADSEEDHNLQLPTQNQEEPHMQKNKSFVQKQIEEPIISMRKATATLNRPQTSEGARRKRFANSTNEVQQNITQKKDSEFQIFDCQANIIQEDDFENNVIIPTITNTLNSPKDLHSIRSGQRRKPTDQAMDNLDNNDRPPSRHKTPPKATGLELPFEPVSSLVNHEIPIKNMYAQIDDKINNNFNDDLDEFDLGFFKMNNKVNLNKFQSKDGYHTDEGKKTNINYKHPQSANLKFNNIGFQNLSYSPLNNQITSAHGFRANEPQIVIKYNNSSSINKNQAKLPVKVPFQTSLGQDFLSLFANDCTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAR5</accession>
    <name>A0A8S1KAR5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0180289</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KLTESCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000382">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="165"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="247"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="283"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8051701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="37558" checksum="ADBAC27C399F799B" modified="2022-10-12" version="1">MEGMGVQHQENVHILQFGFIXEVIKGSYDEKCDIWSLGVLLFTLLSGHLPFHGETKEELYDNIQKANISFTSTIWSHISSEAKDLIKKMLSKQPGLRPNSKEVLKHSWFKKQFKNEEKNSTLLNNSLTSSITENRSIYSLLKQNQGGAKFKKEVKTLLINQLSEAELNQLKEIFKKIDVDNSGTITFQELKQALQAEGSPATFEDIEKLINNGGPPISHEEVGKKKKEFVIKYSQFLASCIDERKFITKEKLSSLFKIFDTDNSNFITKQNIKEAFARNGKQISDQKIDEMIAEIDPNHDNKISFEEFCQMFDNAGVNKNFTEEGSIYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB22</accession>
    <name>A0A8S1KB22_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0190138</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB22"/>
    <dbReference type="OMA" id="ADQITEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000527">
        <property type="entry name" value="Calmodulin-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="6"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="42"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="79"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8052349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="165" mass="18946" checksum="0330C5C204FA8FEB" modified="2022-10-12" version="1">MDPAEKRMKDIKEAFDQFDTDNKGTVSTKELANILKYLGQDPTDEELDNYMKELDPESTGTIDFLQMMKILTKAVKDDDTIDELMASFRVFDVDNTGTIQTAEMRYILIEMGEKMTAQDVKDILKEMDPDDSGMCKYVDYVKKKYEDLQVAKAKAAKLKAKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KET7</accession>
    <name>A0A8S1KET7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0210054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KET7"/>
    <dbReference type="OMA" id="CFENVIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="2">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8053778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="353" mass="39645" checksum="DDAD7EE85045B3F7" modified="2022-10-12" version="1">MQEIYIGVDNPNYLKFDDGEVLGSYPFPHIISKIDVEFNQSHVIGINVTYKVGNGQFVSGQSSLKFKSTPAYVKTFIVPEGDFLYEINGCFENVINSIGFVTYKGYKETFGNPIGVSFRHFSPQNTFTAAKGSFDKWLNFIAFRVVPLPPYAMQQFYPGNNQMMNQVPPQQPQQQQPPPQYPYPTNSYPQQQQYQSPPPPVQQGYTPPPVQQGYTPPPPPVQQGYVPPPPPVQQGYVPPPPPAQQPYTPYPPTYNPQYPGFPGQQPYPYPNYQPYPQQTSTTIVEVIDTNPYGGGYNQGYNNYYNQRQGMGVGAAMGLGMLTGMAVSDLTHHHHHGGIYDVNVYGGHHHHHRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEZ3</accession>
    <name>A0A8S1KEZ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TEP-1 C-terminal beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0200038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEZ3"/>
    <dbReference type="OMA" id="HQFASIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056828">
        <property type="entry name" value="Beta-prop_TEP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051959">
        <property type="entry name" value="PAK1-Kinase_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675:SF1">
        <property type="entry name" value="P21-ACTIVATED PROTEIN KINASE-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675">
        <property type="entry name" value="PAK1 INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25048">
        <property type="entry name" value="Beta-prop_TEP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="72"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="TEP-1 C-terminal beta-propeller" evidence="2">
        <location>
            <begin position="75"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="240"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8052861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="520" mass="60757" checksum="56B39E81F48FD06E" modified="2022-10-12" version="1">MEININVGTYDGKLLGFSLNDQFSQNNTLYSFPASTSLIKTIFQNGRYLFIGGSEELIKVYDVRRRVEITLLEQHNGTITQIAGHSNFLFTAAEDGKVNLWRNKQWAILNTFQCGNPVICIAVHESGKILACATKDQKLHLYNLMNLKRIALKKFHFNIDKIHFISKEDEIQYLLFQSDRKCYIVDCETNKVTHTIDITVQITDSILNQDSLILSDANGIVYMIKLTVDNQIQSKIIVKFVAHQKRIKQLQLFDLNEQTYLASISSDGDIKIWDCLLYANEQYDDIDLKNKLKPIYTIRTTQRLTCFCVSVVQRKQAEQEQQQEQTKIEKPTVVPIAKQIKKQLVNKKPKQFDQNIKVQQVQKKKIQKKKVELQQMNPILQSVLNSPQYSRSIPKKQHQFASIYRVPTKYKARSWKPYTERDKSEDYLVEQMRPKIRIRLPSLQHQDSNKSHQQMLPRESEKEKKIEITTKVMYYLSRMTGPENASKSKKYREKEIQSRLMPGVNSYDGLYDEMYDEDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KL77</accession>
    <name>A0A8S1KL77_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0240019</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KL77"/>
    <dbReference type="OMA" id="EMAYSER"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051637">
        <property type="entry name" value="Ank_repeat_dom-contain_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24180">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 2C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24180:SF45">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE TANKYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8055918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="49978" checksum="7A37185D3B69B274" modified="2022-10-12" version="1">MYNTLSYIGREELIQQIKESIFYFNPQEILEIEQSKDAKIGFINKIKSKKITKRIAATLSFDQISTKDYNRYLQVNNKKDYYKFNMQNYGVYEAALISDIEYLKENIKNKILQQEFKQSLLYTCCRSGFYECVQLLLELGFDIEQKEETNTLLQVASYYGHENVVGLLISYGANNNIKINNINYPLDESRTDQIKQRMKRSSEDNIFKFFQELKQVGLAESIQRSKDDNMIRIYRKIKGVDFQELLKNKKEYSHCYHGTQFKCLKPILDVGLQQAGTKTKYGTVETQDDYFQVYERNDGTNDWRNAIFTTPSPFYAAEMAYSERIWSKTQKYCCLIEVLIKQGIQQEFKSTILSRDFQIYNEPINIEYRIKYNEDAQLNEKIEDINKFIVISALFLKEQYLHEQTNYDDCQQLLDNFSPQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQ53</accession>
    <name>A0A8S1KQ53_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQ53"/>
    <dbReference type="OMA" id="EANFRPC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="181" mass="21458" checksum="395062A662678395" modified="2022-10-12" version="1">MNKARRHSFNEGDGQKLKTLNVLYPNTFVKQHQLDKIREHERKKMERLALQLRKKNTILSSCQKQPEANFRPCQRQLILHNHEIKIQSLSSRHSSYEGIKQIFQRKQFVESKYKIDKLITSPQPNAEKENLCNIYNLRPNLVINKLIDSEFQRKSIMQQFKRLSKIGKSNSTHYSVKSILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KR37</accession>
    <name>A0A8S1KR37_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CHY-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0230308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KR37"/>
    <dbReference type="OMA" id="CLVESHN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="337"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8055692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="57547" checksum="889A62B5BC6D973D" modified="2022-10-12" version="1">MKKQSAKYSKTEPSARRSNKSPTNPNGNPKPEDLLTQSIVNQELLKTQQSFRSSTGPQGQTPSISSKVTQSVTSPVRNSQKRPSQIISKQQLQNMHERSIIKVLGMSMEAVCPKKNHEKNKMLYICQYGDCKADSRLGCSYCLVESHNDHSSQIMEVQTFCKIFDDRKKQFKNMSDTILQVPDKTAQVSQFFEQLRDQILDRLKQIERNILQSIQEQLAWKPLQKEIINRVEILSSKLVFDMTYEELKESLEFIQGKHFKELEYIKQETNNYLSQKISLVEHIWNDYKIQLETDITNQLNDYNKVMFMDPQSKEFAEYKMQFFQQKQLKVNELSQPFQQQLEMFKEEERKAEEKKQEEIRIQQQIEQRKKEEEIKKEKERQKQIQEQIIKQKESQKNSERQSERDKLLKTFPYKLYTEDCSHRTKCNTIPIFQCCNKAYPCSQCHGFVAHPPRIQVPSYRYCMKCLEIYLVMYPTNQAINCLKCQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KS52</accession>
    <name>A0A8S1KS52_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0270121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KS52"/>
    <dbReference type="OMA" id="FSTIQLC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8058249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="25727" checksum="43BC4FEBF74C4CE4" modified="2022-10-12" version="1">MNPRMMLNNRGQQPQQPNGLRNDYQSVVQGIQSLMGISFSTIQLCIFGQMLMEKTKKLLEFIKSGTVTSINKFLTLLRIKQLLKGELTMTQIGKSVVNTSTQIGKQWIIAIRLLLLIVILLIGALLAYMKRKIKEKQETEAQNQSDNEESNNEDAEEQQQMDIQADSTQQPIQQNDFWNHIEENPSNQEQQTQQQQQSTPVPSINYLNDLPGFSNSDNSSKPWMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTE4</accession>
    <name>A0A8S1KTE4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0240068</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTE4"/>
    <dbReference type="OMA" id="GLICKYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="183" mass="21893" checksum="6E213BA3C9C5FC05" modified="2022-10-12" version="1">MQFYQNLYDENPYFNRFFQEHFLYKGFNIQLNDLPHIKTQFNLINKENLNLTWKHKQVNQKFVYMANNQCTNTIIKSYKSNTNFIQSIDLIHIGILLALKYNYEKDLLTQYTFTPLIKISQNIETNSNVDFGLKYHYLNKKGNVHVRLNIFSSLSNRLNIEYCLIGNFKQPYKLQIGLQNSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVY8</accession>
    <name>A0A8S1KVY8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CSC1/OSCA1-like cytosolic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0230051</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVY8"/>
    <dbReference type="OMA" id="FHHESVK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF83">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="458"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="505"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="546"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="650"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="4">
        <location>
            <begin position="266"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="787"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="316"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8055184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="824" mass="96776" checksum="E28B3EC6DB0551EC" modified="2022-10-12" version="1">MNQSSYNQHQSHANNQIDSHDQEIHGQSNQSKQQQQSHPEMQKQSQPSQDQHLHNDQSHHQHSNHGHMDGHHEIHHASNDHHENHYNPLKMPCDWEIARKHALSRRTAQEKKKDPLKTLQIETCPCCGFEVDREDIPYCSDPMALSFLGSGFTLFYNYLRYCIIILFIQLLVKQLHNLYTNFNGSYCSHIKREKMEGHIIEEPYCPDSIFLRLSLANKLDNREALEMMQVLNFISIFIIMFVLIYFRKSQRQIDTTIDEEQLTPADYTICVKNIPTGLSVDYKYELKNLFENYSILDSTKQIIVRKVVLVYDIEEIIELEKKLDQLLQSKKDAIKQSNFNFHHESVKKIDEEIEHLEHQIHKIEEEYETHNTNFSGIAFISFDDESMKQLVLQDNPHTQWERMRSHINRGKLKSLTNNDLQWHGQKLFLEQAPEPNDVDWEFIHITTSEKIFKRVRAWVYYILFESAAFFIIYLISHRLALLGDEAHEEELKGKLDQDTKRKINIMSFSISMTIVLFNKFGVAKIVHYIVDDEKISNKTKFQISFVYKYALALFLNAAIISFLVDIVILKNVKGAGGFIQNESQIFVLNAIFPPFIWCVDPWSLCKNIWRKYIMSKGDKALLTQQEANKLMEEPDYLSAKRYSDVMKTMWFTFMYGTAIPLGTLFSAFGILIYYFVDYYNILRRRTVKESISIQLSTEMIEMLEYIIAWCAFGEMIMTYTFFKEVSKIDILLIVLAILYQQLPMEDISEYLFPVENNEEIKPYAEGSASFDTDYDRENPVTKHQALAEWNKRQQSADNPHPKNQKILAQQDEFGFAGDSHFGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KW80</accession>
    <name>A0A8S1KW80_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0290015</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KW80"/>
    <dbReference type="OMA" id="VYHEKRC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035899267" evidence="3">
        <location>
            <begin position="20"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="5">
        <location>
            <begin position="36"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="4">
        <location>
            <begin position="119"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8059488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="323" mass="36612" checksum="639EBC0526A3CDE3" modified="2022-10-12" version="1">MEKTFLAVGLMMLLGASFFLDNTLEVSDETNKATLFDNWKTMYNIQYPNKIDERYRLQVFIENLNYINAFNQSPEKKTYTLGLNQFADLSKEEFEEIYLKLRVPNSDNINPKDKNFKYTEGAIDWTDGSKIIYPDVKHQGKCGSCWAFATIGAIEMKSQLVYKTLYTLSEQELIDCSGQYGNLGCDGGWISNAVEYVAIRGLSDIKDYPYTGVDGVCKWNRRSFIHFVTTIRIDGCERLAFSLSYDIIPVYVDATLWQFYKNGILTTCDKNLNHAVIVVGISSDGVWKVRNSWGAAWGEAGHIRLAQGDTCGICMAPEIVSLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1H3</accession>
    <name>A0A8S1L1H3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0320153</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1H3"/>
    <dbReference type="OMA" id="YSNMPRS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="468"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8061790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="869" mass="103212" checksum="3B7825230B367073" modified="2022-10-12" version="1">MELYVQFIQSQKKDVLTKRQYKFLADEAITSGSDYDQDANPLEGYKIKEKGRQGQYSYKRRLLSSKHTLKRKFSFNWDRMAIRFGRFYNKIKQLVTLKQPYTFSPDGSMKMLWDLLCLLLVIYEMITIPLLISFEIEISQAFSRISTAMFVFDILLNFNTGVYLEGKLNMERREIFKEYISFWFWIDFISTFPYDIIMDESSSLVQSAKLIRLLKFLRFVKVLKLIRLAKLKKIIDKFDEILSMRPGIAAIVNFCKLFFFVLFFAHVLGCIFHYLAQQEPSDDSWLGDIYFADWQIRYVNSLYWGIATMTTVGYGDISPQTPQERFIGIILLLIACGGFAFTMNSIGFALQEIDGQNKMKKEKINGINRFMKKAGISNQLQNRIRRYIEFVMESKSLVLQDLTNQVQIELANDLRKQVNGKLLGYCDILLKNNSQQFLIEIVLPSMKERVYNPEEVIFDEFDFQQNYDMFIIKSGLVDIFYKKTGIVVDQKRRNEYFGEISFYSNMPRSASSRSVNFSSIFQIYQDKFQQNLKTYQDSSNNSQGRIFDLEQYYLIRDKIIFHKDYGSIGVRCYICQMDDHIARDCEKLHFNVSATHFIKFLKQFDKLHQAAYFRRDRIDFNARKCIQQIRQAQQRFENSKLINLNVKRKTYLEDLLNSYNVAEEDMLDTQYPEYQIYNLKQKSRIKSIKYLNGKITRKFTRIVSYASISENYFGGLTEKIDNFLLQTKKKEMEEKFKQLHLSSISNFDQIQEYNHFYPQGNFSTVLTQYLRALEKGFPKIIEIDTLFGIEEFQNYSQYYCYKLEIDAEIKNRNNNVELNYEQMRLLNMDRRQKKKEKVIKDANQQIQKSLVQLKAYKAINRSFRNLFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2S7</accession>
    <name>A0A8S1L2S7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="18">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PPRIM_AZ9-3.1.T0290144</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 18">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="18"/>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17484">
        <property type="entry name" value="MFS_FBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000180">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000009">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039309">
        <property type="entry name" value="BT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004324">
        <property type="entry name" value="FBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00788">
        <property type="entry name" value="fbt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF5">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03092">
        <property type="entry name" value="BT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 17 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="542"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="565"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="757"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="789"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1288"/>
            <end position="1310"/>
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            <begin position="1316"/>
            <end position="1336"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
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            <begin position="1366"/>
            <end position="1386"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
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            <begin position="1392"/>
            <end position="1413"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1420"/>
            <end position="1441"/>
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            <begin position="1453"/>
            <end position="1471"/>
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    <feature type="domain" description="P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="518"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="19">
        <location>
            <begin position="607"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="21">
        <location>
            <begin position="1253"/>
            <end position="1449"/>
        </location>
    </feature>
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        </location>
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        <location>
            <position position="848"/>
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        <ligand>
            <name>ATP</name>
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        <location>
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        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
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        <ligand>
            <name>ATP</name>
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        <ligand>
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</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8S1L4I6_PARPR</name>
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        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0260102</name>
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                <person name="William W."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
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        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    <dbReference type="EMBL" id="CAJJDM010000025">
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            <begin position="83"/>
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    <sequence length="329" mass="39583" checksum="DB0153C3B1B06757" modified="2022-10-12" version="1">MKTMIKQQYQTKQIQMLFKLHFQIIIQTTENFQIIVQEIDNAQELTDNSFQKPDKIVQETVTYSRKLRNDHENNDSGCPNITEKQEQQEVEKKTKKGQTANNEQKNFEKQRLKIIKELNSTINHLRSEILKIESDDTEKIINEKIQNLQKIQINQSSLAIELCELPIIQQIEQAENNIINLNKVEKDELRKNQNKLIKQIEKIISNRLQKQIGYVKQNQEVVKTEKKLQQKLIKTKNKNEKQLIKYNQLLIQYQNKLKEVENQQWNFEKIDYLKNEQLEKYLDKQMKEELKAKKQKRIENEKLNQKLFNLENIKYEYESDNFNNSFEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5N5</accession>
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                <person name="William W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="CAD8062071.1"/>
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            <dbReference type="EMBL" id="CAD8062071.1"/>
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    </evidence>
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    </evidence>
    <sequence length="332" mass="39852" checksum="62DFCEC4F284053E" modified="2022-10-12" version="1">MQFKSQNICIVNQIQTKIEKHPSQNDNLINKMIISNNNLFVCCIILYEKYKEYYIATVRNLETNQIIIEHEDDADTFHIQFTPDSNYIFFMGEQDYTIYDLQTKQQYKKKFITFDAYKHLKLSFTEDSQYKLIQTYDTLEISNILGEQGQQYQVSPDTFDWRFLYNNTQYLSSMQILRQQKIICLKIFRKKNNKLDLIKSVLLSFEPKYFSKIKAKIWNINNLAIVQHHFGLRVYNLQKNKLIRNLKYPTKQMPICAYQHLEKNQGFIVYSYDNLINPMLTIEFMKFLPTVEYQQPNFFHKNQLFLGQNSALIQLNPESELWQQITFESQNN</sequence>
</entry>
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    <accession>A0A8S1L5Z1</accession>
    <name>A0A8S1L5Z1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0330008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5Z1"/>
    <dbReference type="OMA" id="NQEESIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="216"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="58"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="553"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8062181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="628" mass="73502" checksum="C856AFC0107707BB" modified="2022-10-12" version="1">MKINQPKFKMRKPIGLTLDFLDIQQSQKEQTPKVQQIDTFEEFCSQIPENQQQQELNFSKQSQSFHSQQIKSNNQEESICSEFKQSIINQEKENSQIQYIVFEEFHPQSTIISGQSESIRNSEKQLQEKNTDLIKEPPAPSNKNNTKFSFPQKGTGFTGLKKQLQIAIPEADHTNTSSYEQTMIKQQLKQNQMNNAADMELKQQIFTLQRDKEQIKNQYKLEIIDLNAKIQELQQKLDIEQANTLQLKQENEKLQLKIQQIEHQYSINIDKNQSDQFSISYQNKLSDNEKIQYLVEKLNQKSAINAKLQYEIFKLQNNIELLQTKQIVQSYNINQISDYEQISDSHSPPQFASNPPLNNYKNRESNKASVLMQQKQALLNLSANQAQLDQKKQINLSNMISPQAKTCKASTTIHKNLTTHQTKNTQFKEDTLQHLRKMQSTKNSTSVGELNTFKQDLTNCSNILQKIMRKDSTQSPDKPIQATQKFKEALNSKLQNVPDKHKSYASLLFSLGDFNKPRIQDAYIRQNSPHINEDIQFKSRQIFAYQPHQNSRKQSETIKSEESHKMSTEQPKYYDKYEYSNYQRKDILQLMRDIKSQKLGISAKINYKKNHPKYEKQYNLCQYKGTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6Y0</accession>
    <name>A0A8S1L6Y0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0340184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6Y0"/>
    <dbReference type="OMA" id="FQKCYVE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="155"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8063169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="351" mass="41186" checksum="D248BDCE9FB03D5E" modified="2022-10-12" version="1">MSLEQLQCIEDDRQYYQSISKSTQQQSYQSIATLQSDQSIESLEKLVSQEIVKDSNLKLELDQFIGLLQKVKPSNLKNELSVVMSTEQEEYYSFQQKQPLDRKEVMFEGFQKCYVEAIKIISNLIRDSKEQNYIISYLEKMQMIYIMHIQSYQGMEKSMNLLNSLKQTLIDADQEITLLHNQTAEQQQILQKMQQQLKQSMSELNQERLNSLDLQQKNQQLKHLILVANKQFESLQEKLLQTEQELNEIVSTQSQQIKQLVQTNKNLREAIVFNDKKQEDPKKVNCSPLNSQRNTLNNNQENQYNQLNNQLPINSQRASNLDKGVTNTSPQQSQRCTSNKREWNKCTKIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L7Q2</accession>
    <name>A0A8S1L7Q2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nascent polypeptide-associated complex subunit alpha-like UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0320063</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L7Q2"/>
    <dbReference type="OMA" id="TIKWVIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14361">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038922">
        <property type="entry name" value="HYPK_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nascent polypeptide-associated complex subunit alpha-like UBA" evidence="2">
        <location>
            <begin position="30"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8061652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="72" mass="8540" checksum="0F737D5110186C07" modified="2022-10-12" version="1">MNQKIVMRNEDQEVEVSGTEQPVEIQQETIKLNEAHIDLIVKEFDVDRFQATRWLKKNEGDLQKTIKWIISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LA20</accession>
    <name>A0A8S1LA20_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0340190</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LA20"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="6">
        <location>
            <begin position="43"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8063181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="297" mass="34935" checksum="00115CD60285854E" modified="2022-10-12" version="1">MINYIILIKLAKEFFQQKKIKDQILVKTQNSELILQKKRIILQNKIQNYTSIIHLLGIFSKEPNVTKVPTPVTIFGDIHGYLKQKKIGQLYVVEELVKVGGKHPFTNYLFLGDYADRVAHSVEVIKLLSLLKVKFQNTVTQIRGNHKIRGNTQNYGFYTKCQQKYGNSQGWEYFTAITCIVGTIYYVCMEVYLFLQRVLMKQTLFLEVKCLRNFYISNNMPHLIRTHQLCDKGFNLKFEDRYITVWSEPNYLYRNGNLASIVEEQNQRYFNIFSEIQMIKDLGTKSIMLEADITYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHT7</accession>
    <name>A0A8S1LHT7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0410124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHT7"/>
    <dbReference type="OMA" id="GRDCLMK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20267">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045298">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47484">
        <property type="entry name" value="COMPLEX 1 PROTEIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47484:SF1">
        <property type="entry name" value="COMPLEX 1 PROTEIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="122" mass="14567" checksum="1B26F1AC23490081" modified="2022-10-12" version="1">MNQKSILSHIFDNPFNKLKVGAAAKKTQNKEALRLYRDILKFSIEFDWANKNGETWRDIIRKSARKEFEIAKNESDPFMVMRMIMTSREAMQKTREKLNEEYFKLNTTLSERTKTDEQNKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJT2</accession>
    <name>A0A8S1LJT2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0400116</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJT2"/>
    <dbReference type="OMA" id="YLPAYSI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="118"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8067109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="364" mass="43160" checksum="957182495B470140" modified="2022-10-12" version="1">MQNEKVERLMRSKGSQYIPKNTQTLSPYVRQVPQINSQPHNYLPTYSIQRIQTQSTHSAITPSTERNNSQYIAKIQPSYQQQSVSPIRVIQLSTERTQQVQEQNDIQRNEKPLQVVSIQDMENRYKQKLLQLEQLINRLQIENQRLKINEQNLKSMDYMTKINELELMVKNFVIQEDGLKNEIQRLKQELFEQKESFTLLQKQLQNKNGNNEEIKQLKKQIQQYEIDNKEMQKHLSNKDADIIKLRNILNEKENWIDQLNDQIQELQIVQENYSRVETTVISLQGEVDVWRKKFKEKNEEASELSEKLIMAETSLEALKKRQITQIKEVNITKSNSKTNNNMIQSVDTTNRLNRGSQYSQRQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LN93</accession>
    <name>A0A8S1LN93_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0360013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LN93"/>
    <dbReference type="OMA" id="QKELQFN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035870497" evidence="2">
        <location>
            <begin position="16"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="111"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="246"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8064214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="356" mass="43328" checksum="7DCC8177B9644B86" modified="2022-10-12" version="1">MKIISFLLIITFTTGHLQLLDDLKQTSFGQSLEQTILLQLQYDENKLKSILQEIYSSMNEEQIIDDANFRQEYENCNSNDLQNDIIAIKYEESSIKQKMKLLNNSFELQVSQQKQKELQFNQEQLNQYIKQMEQEIENQVHNLKECDELLELLQYLRFQFEKKLMSQLSNQKSQYLLQLKQEIQDQIKIINKMIKKQTYNNFIKLLISQFEKQDQNYQKLHKIILQIQEIIEQNRNQIRKQFDEDMKHFQTNIQYYQQNIQQIQQEIQLLQEQQVQIEKELKYFQEQLNKIIEIYEIKNQLQIDKDNMCKTLAEDYNDYRNFRNSQIQQLVNILNLVERDLFLIKDHYVLNGQLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPF5</accession>
    <name>A0A8S1LPF5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0360214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPF5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035936081" evidence="2">
        <location>
            <begin position="17"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="291"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8064614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="343" mass="38503" checksum="8FC651C8162A477B" modified="2022-10-12" version="1">MKLSLALVLLVTLASATSTHDQIMAFLQTGTKAMDAIDTVFGLLNDLIQSNKDAQFAADQKNETDEWVGAQTIEQFTKIKTLNQKLFQQSIENRAQFEQELSDTKNYLAWNEQRQDEINRKIQVLLDQQCLSNQLFVRSIKQNREALEVVRLLKQDVAGYIINGDSFELVQVKSVADKLKSYSNTFNEHEINSFLQLANNQEDGSVSRGATLAERVLSVLEGLEANLAASLEALETNEINASWELAGWVSLSEAEVTTLKVEYERKQVYADRLAAQIQAALAQQAKSKIILQESQDALDQAQADLESKRADYAEAKAKRDEENAIIEQVIIIFKKQVASWSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LS67</accession>
    <name>A0A8S1LS67_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0430169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LS67"/>
    <dbReference type="OMA" id="THRYYFS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000051">
        <property type="entry name" value="Protein phosphatase 2C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="55"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="324" mass="36189" checksum="747A17FE382D8046" modified="2022-10-12" version="1">MTHRYYFSYKKSQIETPKRYQYQSIDKLPQLPLITSDVKQSWKVGHKEQSTNLGVLSFPGQLMSGKQKINQDAIIVEKRLNFYGVADGHGVNGERVSGFIRVTLPKYIEQSLLEPKETLINSILQTNNELVNNSKIETVIAGSTLCCGLIKLNRLYIANVGDSRCVLAKQTGNSWQTIELTKDQKPSREDEARRILKAGGRIAAQQDIYGNQVGPLRVWLKTLNAPGLAMTRAIGDRIGAQAGVIPTPEITEYELTNEDKILVFASDGIWDFLSSQEVVSILGQCYSKNISAELAAQKLRNLAVDAWKRNSLARDDITCVVLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSF9</accession>
    <name>A0A8S1LSF9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0420162</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSF9"/>
    <dbReference type="OMA" id="FHEATPK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01928">
        <property type="entry name" value="Cyclophilin_PPIL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000093">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF2">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="5">
        <location>
            <begin position="6"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8068443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="164" mass="18562" checksum="904E93A374AA8686" modified="2022-10-12" version="1">MSVTLHTSHGDIKLELFCELTPKTCKNFLALCAKKFYDNTIFHRNIPGFIIQGGDPTGTGKGGECIYGKYFEDEIVPEIKHDRRGIVSMANAGKDTNQSQFFITYSKQNHLNGLYTAFGKVIYGWEALDLMEKETVDNNYKALNEIKIFKTTIHANPIADIEQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTR8</accession>
    <name>A0A8S1LTR8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trichohyalin-plectin-homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0470177</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTR8"/>
    <dbReference type="OMA" id="KFMDVKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039986">
        <property type="entry name" value="CFAP210"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043597">
        <property type="entry name" value="TPH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28663:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA- ASSOCIATED PROTEIN 210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28663">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13868">
        <property type="entry name" value="TPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichohyalin-plectin-homology" evidence="4">
        <location>
            <begin position="114"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="269"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8071430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="467" mass="56977" checksum="2D1BF525062F6F30" modified="2022-10-12" version="1">MLRYQSEESEAKLKSVQLPSIKQGHTVIGASELEQIKSHISITEQKSKKSIDNNQRVWAQRIEKQRSQKEKEKFDKFQIEEEERRKMDKVETDFQEKLKQDQVVDANNKIFSQRGDVRNMKSKMLMSENEKINEALVRFNKERHEMQMTLEAERLKDIDVKRQMEEQAELEKTQQKDQLKRETYAVLAKQHNEMKEKYFKEYTKLQQEGQLIKRQAEEEEQEKEKKKEEEKLKKQKLLAERQKFMDVKQSIQDEEQMLLDKEAKDRNHFENKKERVLEMRKQREAQKLERQMQLKQKIYDMRVIQLKEQEDNYMQRVQKHAEELQRHEEEVARQKEQEKIQMIKEGETFRQRQLKLKETVKEKEEVDANKEQVLKINALQQLSVIEEQQKEILKKRNKELLDYQKIQIEQKKQARRDQYMNELKESKQMELRAQQYRDTFMNWAKQQVEETREQGLNLYPLLKTLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LWZ2</accession>
    <name>A0A8S1LWZ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0450045</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LWZ2"/>
    <dbReference type="OMA" id="EEWVQFY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000537">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="56"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="312"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8070073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="42895" checksum="C27B6C281585F87E" modified="2022-10-12" version="1">MGMCQNKESQYTQKQPNFKCDVPEEEVEDDKIGEATQLSIEGETLTSNQKLSLQDFELIKVIGRGSFGKVLLVKKINDQQLFAVKILKKKMLSMKKFHDQAIQERKILSLINSPFMVKLHYAFQSPSRLYMVMDFMQGGELFLHLRKNSKFPEEWVQFYAAELLVAIDLLHSSNIIYRDLKPENILLDKSGHIVLTDFGLSKLGFEKNELTYTFCGTPEYVAPEILYKKGHTFAVDFYSYGALIYEMLCGIPPHYSKNKKEMLQNRCEKPLEMKSCFSFYAASLLKGLLTKDPGLRLGSHGIKEIKKHNFFNGINWQLVEQRKLQPPIIPYIQNVQDLSNFDTAFLQQPIVDTPESKQNENFVGFTYYQQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYK7</accession>
    <name>A0A8S1LYK7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460279</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8071065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYK7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="78" mass="9556" checksum="D9FA2A0578F317FB" modified="2022-10-12" version="1">MNTQTSLQLYSAENSNTLKVQEQEHLIKYIQMLLMDMMMQIKIKCSETKYTQLIFESKFYWFFKNQAIIDIPKYYSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0C2</accession>
    <name>A0A8S1M0C2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0510264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0C2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="37633" checksum="C9FE8A36CF9D4604" modified="2022-10-12" version="1">MDLRNRNTRIVLIQMLEKQLKSIMTENITKVKYQEQLTRFQISEDQKKARKKQHSPSPQLMRTHSQSDHIQFYERQQSQLKIKNERLYQSKEKSILKKLQEEIQEATFNPKILNFKGQNTQKNNLYQKGIKWMQQKAEYIDKLKESSMVQISQENLFKPMVNKVSEKIIKTTNQTDFFQRMNKNEDKKKEKIKRLKTDATPSFKPKINDRSKRVQSSLNKDILSVSYNTELMEKIRNYNQRELNQSCHEYSKQRNCSSVLSQSIEIDKQTNDYPSFRNSTTIKKQPKLQIDCNTPSQNKTEMLYESLGNQTYNYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0E2</accession>
    <name>A0A8S1M0E2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0480175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0E2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="158" mass="19416" checksum="874B8D98629640F6" modified="2022-10-12" version="1">MIDYYFNFKRDNFLSQDSKNYLQIYNSIKRNQIGTNIKMIQSFNQSKNRFCQVNLIIQKTDSFLTYFHQKKWQREKMFTYNEQKCSMFYNHEQNRLTFKNYIAEKVPKINKIVDIWIILNRNKCLIFHYIWIIILKLLKSQWQGWGKAVQNCLINCII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0U1</accession>
    <name>A0A8S1M0U1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0480264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0U1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="142"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="187"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="253" mass="30134" checksum="34B254F92CD9BC85" modified="2022-10-12" version="1">MQNIKLQFNNQSSFNRIYDYLDQIALAPYPSVPQKCVKNIPAFPQIILNSIQPLVYEVNKEVKPIFNRSIQNLYYRIKQNNNYQLNLKPFTFKLIQNHSIRQSEKYRTITVNKDNSILIAACDEYIKKFEFKQDQLRRTKILSEHKDSVTTLNFMNKSNQFISESLDKQIIIWSTNHNNQWISSQKLRGHTDFIFCIVLNKNEDLIILGSCDKTIKSWYKQQQWLCSQTIIDHTYRVYGLILNEEEQIVILWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M118</accession>
    <name>A0A8S1M118_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">acylphosphatase</fullName>
            <ecNumber evidence="2 5">3.6.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0510093</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>an acyl phosphate + H2O = a carboxylate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the acylphosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7" evidence="2 5"/>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M118"/>
    <dbReference type="OMA" id="PLNEMKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000011">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10029">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10029:SF3">
        <property type="entry name" value="ACYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="7">
        <location>
            <begin position="7"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="22"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8073507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="102" mass="11923" checksum="04E6AB1F34ECEE78" modified="2022-10-12" version="1">MIKKYFSFDFQVIGKVQGVYFRKFTKEQGTNLGLVGWVENQKDGSVKGVVQGDQKKCEEMIHWLSNVGSPKSKIEKMIKTNEREIDKLQYKDFSVRDDKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8H4</accession>
    <name>A0A8S1M8H4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">NEDD8-activating enzyme E1 catalytic subunit</fullName>
            <ecNumber evidence="8 10">6.2.1.64</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0520011</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of the dimeric E1 enzyme, which activates NEDD8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.64"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.64" evidence="8 10"/>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8073965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8H4"/>
    <dbReference type="OMA" id="HIIEYVI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.520:FF:000001">
        <property type="entry name" value="NEDD8-activating enzyme E1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF6">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="11">
        <location>
            <begin position="26"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8073965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="414" mass="47288" checksum="AA6FB365C414AB71" modified="2022-10-12" version="1">MNSILNTYSPLSKDTYNEYRGQIMIEALSIQKVLVIGAGGLGCEILKSLALSGIKEIHVIDLDTIDLTNLNRQFLFRMKDVGKYKAEVAAEFIMKRIPSCKVIPYTKKIQEFPISFYQEFSVIIAGLDNVEARRWINRVVIQMVQRDENDKVIDETRHYLIDGGTEGLNGQARVISPFETACYECTISQLPKQIQYQMCTIASTPRLPEHCIAYAYEILWPKQQPDLKLDKDNFDHMNWIYQKALERAQQFNIQGVTYKLTLGVVKNIIPAVASTNALIASICTVECIKILTGNGSQLNNYIQWYGQNHQTGIGINVIQQERLEECTECSIQSLQIKVKRNDKLSVLLTLLPQGIELYCKGEILITNNNIRKQAFDHLLDKTFQELIDQKIIDENAQLKALDKNKTINLKVEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MA24</accession>
    <name>A0A8S1MA24_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0530039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MA24"/>
    <dbReference type="OMA" id="IFGIINQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000551">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="132"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="337" mass="40115" checksum="7BA52D0AB4B156EB" modified="2022-10-12" version="1">MLSKYSIRSLTLLRFPRYRFSQQQQQQKEQQDEWDERTIEAEEANPTLENKEKAFSYFRLFSRIFWWTTSALFGYNLYLNNYKTDPTQELGYQKQINDAAKYCQDQYQAFYDFMTKPAIDKLLPDIPELPFGYEIPKTLVLNISGTLLHMDYVFGVGGEIKRRNGLQRFLEKLPKMYEVVILSDDETMFTQQITQKLDPTRQIFAGAFGRESMVFEKGRYIRDLKYINRPLNRVIVLDSDPERMYQYQDNGIFIKPFDGKQNDEVLKDVLLLLEHLSKPQIKDVRAELRKFGNFDPQVKYLDEVKAREINIKQTMNKGIFGIMNQRKNPQFEQSRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MD68</accession>
    <name>A0A8S1MD68_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FPL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0590218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MD68"/>
    <dbReference type="OMA" id="AYEQLQC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901096">
        <property type="term" value="P:regulation of autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039272">
        <property type="entry name" value="CLEC16A/TT9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019155">
        <property type="entry name" value="CLEC16A/TT9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481">
        <property type="entry name" value="PROTEIN CLEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481:SF0">
        <property type="entry name" value="PROTEIN CLEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09758">
        <property type="entry name" value="FPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FPL" evidence="2">
        <location>
            <begin position="36"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8078207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="872" mass="101940" checksum="F76FBDCDB674BA0D" modified="2022-10-12" version="1">MQKQPPLQSDPDSLVDAYEQLQCKMSHNNYDVVDQIGQICQSVMWGDKNNDTFFDYFCDQKTMSLYLDLVHTGQREVVVAVIKAITMLLQNIQRPVCINYILSHSALNGIITADYNFRDDEIIAYYINLLKCVSIRIDEQSLQLFYNLKFSTFPMLMKAICFYKHKENLIRTSVRNIVLGITKINIPQLRRYVSSFPFTLFYVQFSCYMRDALFYIGGLIQEGRQESHKIIKLLLDDQLDILYFLEDLLKTNVDALPIIVNSIMVYGFVPTIIMGVIKSGQQSIKQNQQPQRQSMNYQQSQKYQHNRGQSLGNFTFTNIFSSSQQTNNQQQQQIIDIKFCLFILIQIYKSFSFTQFLDLLTLFILGKDPEIVDFITDLNSNSKLNSYEKDVSMSETWIIKEKTFIQAYSQFIYKDCEISGDININDKDELLKGVSSYETQSIKRELNCEHFKPLINDSKRYEDLLSITDMCDITIVKSQNKSKTSALSFLERNDKNKVNGNTNNNKTPFYALMKHLESNDDTVVFMVLLFIKTFRHQKNISHSILEQVGMITPDKNMELYKNCSETSFFMQDKLLGILETGLPPYRLGTYILSIQMLIDFCRTDLESYDLRYKDKFKNIVLTHIQKINEYINNPLEAYYVIEFFYTEYKVLNKTSISIWPQTHIQLLYEMTPKGVCQIDIKHNESIYFMEPANKQDKVRKDMLILLILKYTTSVLIGFEEQEQKILDNPKPFGDGPNLKYVSGNVYNLQEEPYCQFIQHICKYQNIDCCILEDSYNLVVMDLTQGQSAAKCIVSHNLKYTDEMVERLNLKMLHVTYRFENQNNVFDLEFQNKQCCQAAKKMTEQKKVALRKEEVDTVTTYLQALKNTLNYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MII5</accession>
    <name>A0A8S1MII5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dolichol-phosphate mannosyltransferase subunit 1</fullName>
            <ecNumber evidence="13">2.4.1.83</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0590250</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a di-trans,poly-cis-dolichyl phosphate + GDP-alpha-D-mannose = a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + GDP</text>
            <dbReference type="Rhea" id="RHEA:21184"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 13">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.83" evidence="13"/>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MII5"/>
    <dbReference type="OMA" id="KCFRREV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006720">
        <property type="term" value="P:isoprenoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000036">
        <property type="entry name" value="Dolichol-phosphate mannosyltransferase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398:SF1">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="14">
        <location>
            <begin position="5"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8078271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="236" mass="27191" checksum="6A1AACEF54E94D39" modified="2022-10-12" version="1">MSKYSVILPTYNERENLPIITYLIFEMAKKNNLDFEIIIIEDNSPDGTLQVAQELKKVYGDKLIIHFREKKLGLGSAYMDGIKLCHGNFVVIMDADLSHHPKYLVDFIQKQKQTNCDIVTGSRYINKGGVMNWGFDRKLTSRGANFLASTMLGVKCSDLTGSFRLYKREVLEKVIKDVVSRGYAFQMEIIIRARQYGYTVEEVPIVFVERIFGESKLGASEFQIYLKGLWKLLWTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ML29</accession>
    <name>A0A8S1ML29_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0650064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ML29"/>
    <dbReference type="OMA" id="IYSVNMA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="66854" checksum="4E9156DC13CCBF4C" modified="2022-10-12" version="1">MMKGSQQNVLLKELKEIPIYSDNWFWKLRMRNQALLQFSIIFIVMLGLVLGIYSVNMAIIVPLLEQSSFDIYNNNAKHTLQSHLRSYDGILDGIFNINIHQLQILHQIYSQSYRNLEKQDKITLDYRAHMNYAGTNQMPQILVNQPPGSLQKTHSFMCYTNKTNITYPMNKVTLINLKIQEIMYPIGHILDANKLNIKSLFYVGTLEEPQVYFSYPCTNFKNAIYGLNIPKRPWFLKGVSVYNQSNFRDYDYQFTDPFLQFTKDKIEVTLVMPLLDQNLNLKGVMASDLGTDLNKFVVKTDQNVHMVLLANTKGLLIYHSYNVSLSLLPIYIFNTSLTGFTLDDWTSIKETKQSNCSSYPKNSSLLCRYNSVYDKDLVITIHEIKKFNYLFMIFHDTTDYTNYQLKLTQQMLNKLQSEAIQFLEIALGIFLAAMLIAICILILIFYPIQNIIDNCHYIMGIKNKQNVHKKILLTKFFMPFLNPQLQLLFLAYTNLVKRFLSLSHTKGNLCKTLEALQYPKKIKQRAHLQLNRYLKKSMNLIEQNKMILQVSTHIPSQLMGRFFDNIKKKFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MP91</accession>
    <name>A0A8S1MP91_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0670051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MP91"/>
    <dbReference type="OMA" id="NQICAMR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8082050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="653" mass="75502" checksum="FACFB56829B18F70" modified="2022-10-12" version="1">MGSQESKQEGQIQDSRYGKATLIRKYNQICAMRKVIIQDESQHDGFIKRAKVDHPNLVKILDSGIQKEQELCTSYNIMYVISEYYSTSMHKEIVNRKGRGAFWTEQELWNQLLGLVNLLMTMQEAHLVHQNIRPLTISYVNENTLKLCDNYFQQSAFQMAQQNPENQKLFELSPKLLEAINQNNQFPRHNLYKSDVWSLGMCMLQASLLDHCRDCFDFEGGHVDVSRKLALLKKNYSIKYEQVIVSMLQMDEESRPDFNQLMNLMKGNPDAGISKSLENSLINSSAICLDSKSPMNLKEDIIPESLNQLKEQIESKPSKIMVELEQLRIKSKMMIKKPKKKELGFYQYQEQMVIRDPSKSAIQQQKINSYASVDYSFNKPMVNSQLQFTNSSQKKQFTDKEKIEQLIGNGLQSYSIDPFIMPNTSIKPQISQVESQKPIYSETTKTTQQSNPRVITIDTHPSCKSIPLSYTEIPISYVVKKDINYRQEMEQMIEMNKSRCFITEQYQDGSSYVGETYNQLRDGFGRYQFAEGGYYEGQWKQNKMHGYGTLFYGVGQKAYEGQFENDQFSGFGTLYNKEPTKLNTSFDCSNFDLIGNYWVKYVGNFKNDLKDGQGALYLSNNECYKGEFFQDYVDGHGTFGSLIGVWCKNKLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPW1</accession>
    <name>A0A8S1MPW1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">60S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0650190</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPW1"/>
    <dbReference type="OMA" id="MDFYCIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000004">
        <property type="entry name" value="60S ribosomal protein L11, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="11">
        <location>
            <begin position="35"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="12">
        <location>
            <begin position="92"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8081362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="22566" checksum="120339C88BE0C627" modified="2022-10-12" version="1">MSQQQGKGGKAQQTKTDGKPAAPAKVVKPQETKENVMRKIRIEKLMVHICAGESGDKLTKAAKVLEDLTGQKPVFGKARYTVRSFGIRRNEKISVFCTIRGDAAKDILFRGLRVKEMELKKRNFSDSGNFGFGIQEHIDLGLKYDPYTGIFGMDFYVILSRPGLRVAQRKSRNARLGTAQRVSKKEAMEWFKQTFEGNVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWD6</accession>
    <name>A0A8S1MWD6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0610140</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWD6"/>
    <dbReference type="OMA" id="LSMNCII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000150">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948:SF2">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE DDB_G0269404-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="15"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="75"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8079134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="208" mass="23984" checksum="8F09C4BDA931B1DD" modified="2022-10-12" version="1">MIVNNYCLSMNCIIAPLNGCGGLYLGNLDAAKDSQLLIQYNIGAVLQVLDQSVPVKGAQKLWIMAEDSEEFPLNKYFEQAIKFIENQTKKTNVLVHCYAGISRSAAIVAAYLMQKYDWTMNQAIIHLQQKRRIVNPNPGFIIQLQDFQQKLSHNNQESNLSQKNIKHDYQNDQQQKINSPLHKLSRLDEFNNKLDQFRNQLRNKQKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWI1</accession>
    <name>A0A8S1MWI1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0700177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWI1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029147">
        <property type="entry name" value="CFAP77"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14825">
        <property type="entry name" value="CFAP77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="215" mass="24786" checksum="41AC43BD62863395" modified="2022-10-12" version="1">MQTSPPFSQNHTNPLLMKDDVGKSKPSTYNLPNQDFVYGQPLVKDKEGAKEVTMTWKFHKESQDQVPNRDFTELNKQSIHNGFVKAHDMYKFRQTNDARLKIKKGTNIQAIELPEEEFRYGRKNRPSTPMKLVMGNSYGIQAESIILDKYQCRANSQDSKLSSSIVKGNKASQLYYDTNHKKLAFIQGIEKKEPFKMEKFKTVNSKINTNLSTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MYS0</accession>
    <name>A0A8S1MYS0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tubulin/FtsZ GTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0740166</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MYS0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000044">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="5">
        <location>
            <begin position="31"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8085498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="425" mass="50079" checksum="F63CC11D5460AD7A" modified="2022-10-12" version="1">MIHIHIGGAGVMIGDILWKLYHKEEPDMSYKHSIFEEVKNGYVPRALFVDLDDRMITQIKKNRNFHYHPSYLINSKEDGSNNYSKGYYTLGKDLHPKVEEQLRKLCEACDRVQQFIFTQSICGGTGSGYQSLLIHEISKNYNKTFQTGFSIYPSAKMNNNTVYPYNAMLSIHQLVEYYDAQVLFDNETMYQMLQNTIELDHFDYSHLNNLIAQTISSYTSINRFCNKNNEQILSTIVPYPRIQFITPSYGPLIDINDYKRNYLSQKQLLEYIFNRRSKLYQSEITPFNLATSLISRGLNADQIQKEHQNQIYYHNYYEQKTNYFVSNQKNYSVLEQLTEINQTQTLFSNDLMIKRKLEKIGYDFDILYSKRAFTFWFVGEGLESGELSECRENFEQLMMDYQNLLKIDDDIDEDISNESYDSQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0C7</accession>
    <name>A0A8S1N0C7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0730131</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8085072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0C7"/>
    <dbReference type="OMA" id="WTRIISL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="61"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8085072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="441" mass="51912" checksum="67F3873FA6402387" modified="2022-10-12" version="1">MQNDETNDTTTQINDIDVQSQQKETENIQQDNQQEVIEQQTLEQQQQSNEALLRQIDDDEPIFILVSNPSEVKSSSMNKYLVYTVKGKDALGNFEVQRRFNEFYTLREALIQIWPGLYIPPLPDKLVNPTGEQLLERVRLLNIFVMKMAQIKYLYYSEEFVQVFLRSTQLDVCKQIQNLPKPNIRSQIEKFKHIFELDQVQEPDGDSINKINQMQRFCKQSQQLLNKLFDQAKSLSGSKRCLKELFHSFFGYGLQEFEQQILLPCLNNEKQLILTNPDAQELKSTLDYLKDQNQISLDKIQDLISTEQRDMEAFLQGFQSRESLLHTKSKVENKLREQQEQLTKVMIAKGGSFGKSQEEQRQKIELQIVESQNDVDSYKYYYQLVTHVLTMKAIDKFKKDKHESYKRIIRDLAQLEIENNQVMQEVWTRIISLAESLRVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0F6</accession>
    <name>A0A8S1N0F6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DM10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0690180</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0F6"/>
    <dbReference type="OMA" id="RFSCKQP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043014">
        <property type="term" value="F:alpha-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060285">
        <property type="term" value="P:cilium-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.170:FF:000004">
        <property type="entry name" value="EF-hand domain containing 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006602">
        <property type="entry name" value="DM10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040193">
        <property type="entry name" value="EFHC1/EFHC2/EFHB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086">
        <property type="entry name" value="EF-HAND DOMAIN C-TERMINAL CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086:SF9">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06565">
        <property type="entry name" value="DM10_dom"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00676">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51336">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DM10" evidence="7">
        <location>
            <begin position="94"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="DM10" evidence="7">
        <location>
            <begin position="253"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="DM10" evidence="7">
        <location>
            <begin position="411"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8083236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="513" mass="60459" checksum="EB43AC149A2968F7" modified="2022-10-12" version="1">MASAFPKLPGFVPTQEIDKPNFRKVSSTKQEQNREINHLSNKEYAVPRKQPIQIPPQTGMFNPDYMSTTHAMHLPKNSNNDSEELYQPSWVKMDRHVLRFSGYFKEAVVESALENYRIRKITIFYYLEDHSLSITEPKQENSGVPQGAFLKRQKVLRADNSKTFILPEDFRINQDIIIFGKTIRLYDCDQYTREFYELQGIPQEQSFVPQQDSFETKTTTKFIPQKDTVMKDYLEHKLGGGKVTSQKQFLENDRKVLKFYVFSDIDYILHYYLADDTIEIKEINSANSGRVPFPMMLRRQKLPRKFSLNQPGQTYAEDFIRPQDIQYGQPLIIYNRKVLINGCDQFTRQYYQEKFNVDFPLGGQEEYVHQERSNIIIPPHNGIGDEQDSLGYIYRLQPKPPKKDFFKWVDNQVNLRFLAMFNTTKPEDKDRVFVITFFLNDDSLLIYEPTVRNSGIPDGKFLEKKKYKNVNNNNEFFTPGDLIVGNEVQINGWKFQLLDCDEFTKKWYSENFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1T0</accession>
    <name>A0A8S1N1T0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0780068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1T0"/>
    <dbReference type="OMA" id="FRNDYAL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="251"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="665" mass="77148" checksum="8B70FCED9E759AE5" modified="2022-10-12" version="1">MSSYIIAGSSDKVDTAECENMANLIRQYYPEISFKIVLKDLTQWEQYADEICRIYGFKQKSNPIIYTMDGCLIGDRNAFISLAESRFGFSDPKLRQGPITPDVNKKIAKQELDLKLKLQRNKKTLIEKIEQQMLIIYSNNIVQPTDGVFKQSFKSGVKEKLKTWYDSLNNLIRTDVKRNLSTDSIYSSSFRNGMKFLYKFDDRLRPIKKLQESHYFEENKETIQESNYVDVEVKEDGKLVIKQILWFSETARFLTLEQEQLQQQLQELAQQKEREQSHKNSIISSDIKRDNNELNDINQSPKATIQTQPIVEQPNEDGEQDIIKNDQIEDFQQIDQQVTDQQQEQINPTIEQQELDQVQSIGGGGAIMDLQEDIETILNNNQEQPDKIDVSELFVHKFRNQYALILHPFPLMDGQMLLCKPIDNHIDDYSCIKRGRINSYKKSSAQSNCEGLYEHQLDIFKSITIQDCEVVTKIINKCDALVVLNILPQDLESFLPLSNGHYSIILRQHAELNKANKKLFKFLNEKKVQVNYKQLMPIEEFILDQRSQCYEVQREVFKLKSLDIQHYFAFPPQILTAESVMDIILKIISIILDYDHTAALKMGFNLLISKHIIFLCPTFSNYDNIDGFKLHLQAFTAMGFLNLPTGSSSINFFDLLQKAQEKVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N445</accession>
    <name>A0A8S1N445_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">aconitate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0800095</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072350">
        <property type="term" value="P:tricarboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01586">
        <property type="entry name" value="AcnA_IRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01580">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000001">
        <property type="entry name" value="Aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000002">
        <property type="entry name" value="Aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000005">
        <property type="entry name" value="cytoplasmic aconitate hydratase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044137">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01341">
        <property type="entry name" value="aconitase_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006757">
        <property type="entry name" value="PRK09277.1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009520">
        <property type="entry name" value="PRK12881.1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="9">
        <location>
            <begin position="89"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="9">
        <location>
            <begin position="631"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="10">
        <location>
            <begin position="1171"/>
            <end position="1297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8088117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1368" mass="150833" checksum="393717D270C29406" modified="2022-10-12" version="1">MLSDAQNKFLIMIRFTSRFRFGARANPYIKAQKTLKVDGKEYKFFSLPALGDSKLNHLPYSIRVLLESAVRNCDEFAVTQKDVQNILNWETNAPKQIEIPFKPARVILQDFTGVPAVVDLAAMRDAMKRLGGDPQKINPLCPVDLVIDHSVQADVSRVPRAFEENEKIEFTRNYERFEFLKWGSTAFKNFLIVPPGSGIVHQVNLEYLARVVMEEQGYLFPDSVVGTDSHTTMINGLGVTGWGVGGIEAEAVMLGQTISMVLPEVVGFRLHGKLPSNVTATDLVLTCTQMLRKRGVVGKFVEFFGPGVETLSLADRATIANMAPEYGATMGYFPIDHKTIDYLNLTGRPETKVRQIEAYLREQGLFRDYKSGNDPHFSGEVLDLDLASVQPSLSGPKRPHDRVPLGEMKKDWSSCLNNKVGFKGFGIPQEKQADVVEFTYQGQKYSLQHGSVVIAAITSCTNTSNPESMIGAGLLAKNAVEKGLKVKPYIKTTLSPGSNVVTKYFEESGVNKYLDQLGFTTAGYGCMTCIGNSGELDNEVADAIKNKDLVAAAVLSGNRNFEARIHQQVRANYLASPPLVVAYALAGTVNIDFDTTPIGTDKNGKSVFLKDIWPSREQCSKAVEQALKPQMFREIYSKIAQGTERWNQLKVNKTDLYQWKPESTYIHNPPXXXXXXXXVGTDSHTTMINGLGVTGWGVGGIEAEAVMLGQTISMVLPEVVGFRLHGKLPSNVTATDLVLTCTQMLRKRGVVGKFVEFFGPGVETLSLADRATIANMAPEYGATMGYFPIDHKTIDYLNLTGRPETKVRQIEAYLREQGLFRDYKSGNDPHFSGEVLDLDLASVQPSLSGPKRPHDRVPLGEMKKDWSSCLNNKVGFKGFGIPQEKQADVVEFTYQGQKYSLQHGSVVIAAITSCTNTSNPESMIGAGLLAKNAVEKGLKVKPYIKTTLSPGSNVVTKYFEESGVNKYLDQLGFTTAGYGCMTCIGNSGELDNEVADAIKNKDLVAAAVLSGNRNFEARIHQQVRANYLASPPLVVAYALAGTVNIDFDTTPIGTDKNGKSVFLKDIWPSREQCSKAVEQALKPQMFREIYSKIAQGTERWNQLKVNKTDLYQWKPESTYIHNPPFFQTTELNPKPVQPIKNAYCLLNLGDFITTDHISPAGSIAENSPAGRYLKSKGVSKKDFNTYGARRGNDEIMARGTFANTRIINKLVSKVGPQTVYVPTGDVMDVFDAADRHMKEGNQTIVLAGQEYGSGSSRDWAAKGPYLQGVKCVIAQSFERIHRSNLVGMGILPLEFLQGENADTLGLTGKEQFTINVNESNLTLGQTYTVETSTGKKFQAKSRLDTEVEIEYYKHGGILQYVLRKLVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7T9</accession>
    <name>A0A8S1N7T9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0820021</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7T9"/>
    <dbReference type="OMA" id="NELTGMY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="218"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="306"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="507"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="576"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="623" mass="74403" checksum="4D5B4E884B85BC08" modified="2022-10-12" version="1">MNTALRQPLSSHNSIDKRENKPDYKSDLKSFSFIKSNTLNVQSQQQSQQQILLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLLQQQINKTPSQTTLTTNTNNKINNQIQDLETFIKEIDRLQLENQQLKVKAQEPQLIIQSDPKVAIENQDLKNQVDYHKQKYELQEKTHNETVRSMDLNWKQDVVTMKFQMEQQESKHRQALKQEQVQAKQEIARLQQNLTQISNSENKNQNLVQVLERQLINLKKSNNILEEKLSQAIQQFDSQSKDQEFTLLKQQAIITSQEQHVSELKSKLGCQRDQNQGLNNELERVKEITEQKKQNENMLTNKIKQVESDFDHLQQTYQQHKEQLQLKINELTGMYGKSQEQICLLQQQQIREQNLIKRLEVNLENEEKNHEQEVKDIHKSKQLLIEFLEQELVGYKEQNQQLKLVNQKLENQHQEQQQYFGKNFRELECKTNMLGEECSRLNNIIKQKDDQIYRLQVQLKDLMNLNGKNQNQEELLESLQIKEGEINILKQQNESLINDLSRQTDNYRSLAGQHKQLQKFAEDAKMNNITMNEFMNKCELQEREVERLRMKVLEKSQLLEKVQKENLEYQAKLKSKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N848</accession>
    <name>A0A8S1N848_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0760049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N848"/>
    <dbReference type="OMA" id="DERIFQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="238"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8086186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="624" mass="73437" checksum="3BEB95D705762154" modified="2022-10-12" version="1">MQIKKIQEINCQCCVYRNNIRILVKGEKGLLRKVVISLYERYPAQAAPIIKQPIKLKQPTLEQMFSRQKQAKEETKEVIEKKPLEMEVYIKVYCNGEELLCFNLDESINLSKSEVRQQTLNLQFADKILSLYECPNEILEQFRSVLIQLQNDKKQLMNHRKKIQNIQTQEIQQQEPEIPPRDTQEKRELNEIQKLKLQRRLLASQQQQQQQQQQQSEQIQKQIIKTKSSEPVNTIKQLNIKFQLPLFIWTQILCYIDTRQLLRLRLTNKQFEQSINNIVNKLSYKHVGEIPYETLCRSLTKFSKLKHIIFGSLKFIKPTQLQDIRISSIPHLLSLDFKDYHVLTDKLCSKFILQCVNLKTLVLPYNSKITSDTITLISQKLTNLEKLKLVEKDKGQLNEEIAFTSFLSIFQLKQLQYIRIPFLQVEFFMKYSQLNETNPFNSIKQKIKFGTIIFSILNSNQISQFLNFLQINNEINKLSIGELQMKNQSALKYTVLFDERIFQQLNGILKFSFGGLVDDEFCRQLCTHNPEIKQLKIRSKMITDDGLKNIFLKCKSIEKIDVAFCSMICGSGFEYLTDKSLTLEYPKIQIAVNLDDYNMQCLEELLQRKAYDCKPTLRKKLKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCX9</accession>
    <name>A0A8S1NCX9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0780157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCX9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="137" mass="16406" checksum="817C63847B15E011" modified="2022-10-12" version="1">MIFLFFGIGCTPQEQMDIFNRYKSGTYIIHGLLLITTKYSQQKRLSFIIKCIIQQIHKRNLQFFKQNYIILTNIQILLHIIVMQEQNHLECIKETLKTVKEVGKLVRDKKVTTEVDVYYFALCSKMLFRELIHFLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEY2</accession>
    <name>A0A8S1NEY2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">V-type proton ATPase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0860152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase C subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEY2"/>
    <dbReference type="OMA" id="KSSYIQW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14785">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004907">
        <property type="entry name" value="ATPase_V1-cplx_csu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03223">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="1" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU364010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="405" mass="46899" checksum="DE0012B66906B4B4" modified="2022-10-12" version="1">MVYYLIGAPHKSNEQSAWLEMEKHVLTSGISRQAKGRILQAEIGKFKLGNLDHLMFINDSLLKQESVVESLLKKIERQYLDVTEKASYDFTIELKEGPQSIENFLMSFKWNDQTFPRASALTELVKAIQSRATHVETDLRQKSTSYQELKNQSQQVAKKEGNLLVKDLVDVLKEPIVKPRDFIYSDYLTTLVAIVPKTQIQEWLACYEFLCENVVPQSARQFQIDDKDNLTIWRVVIVKQSFDKDHDLEVVDKDDEDKAKKQKQSPVDEFIQKARDKLRITVKEFGYKSQESKEREKLRLDLKSKSDHMNTTLKQACEKAFSDLYITYMHLKVLRLVVDIAMRFGAAEPSIQCILKPDQGKEKKVQQSLLKLYADPSQVGLYGTKEELEDTEDFFPFVYVPINIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFF3</accession>
    <name>A0A8S1NFF3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0860232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFF3"/>
    <dbReference type="OMA" id="CKLITEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="36371" checksum="CC2B077BFA9621CD" modified="2022-10-12" version="1">MRGRILFSNLQQGGMQFRIETKLGQNIRKNFFQKAYGQLDNNSKDPNDEMLYLKEYEKKVIENNYKNIDQENDSNAIIIAKMKSQLFKLITNINMLQKYPISNFLLLLLIMINHSYIQQPTIHMNDIAVQKDDELIQNSLKNLPRFKKMEIVGEIFALLVLILCWAFFHQSFVYLNEKVPTEFDYNGNAVRYADKNILFALPAVMTISYIIFTILQFVPHRFNYDCVGLTVFNAQEIYRTTRITLLSCKLITEFLFTYITFTMLQVVQYQCEPQRMYYAFVFILPYLIIGVFYYRKLKNISTQPQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKG7</accession>
    <name>A0A8S1NKG7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0910034</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0910035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKG7"/>
    <dbReference type="OMA" id="TNCHIGS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018188">
        <property type="entry name" value="RNase_T2_His_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF22">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00530">
        <property type="entry name" value="RNASE_T2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036434443" evidence="1">
        <location>
            <begin position="17"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8092602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="240" mass="27277" checksum="0C537C7A651D4800" modified="2022-10-12" version="1">MSKNLIILLLITIGISYEVPNMFYTFQLSWPGSICLTKQCYKENVGNYKGQSWAIHGLWPGATNDSGDCEELEACTNNKFKESNLSKTTMTNLDQAWVGFFNPSGSFRAHEWNKHGTCWDEKDDLVPQVPGMNIQEEYFQTTLQLWKSYNIYDILSAAGIKPDDHKFYDTDSILDAIENEIGSSAQLLCTKDSNNKLLLVSVSFCVDEKYEPQRCPCDVYGGIPCGSRKNDKVRYPVFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NL19</accession>
    <name>A0A8S1NL19_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0870133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NL19"/>
    <dbReference type="OMA" id="TAKFKAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000343">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022122">
        <property type="entry name" value="DUF3657"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF5">
        <property type="entry name" value="DUF676 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12394">
        <property type="entry name" value="DUF3657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="1">
        <location>
            <begin position="375"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="639" mass="74186" checksum="C035B9DC2D202522" modified="2022-10-12" version="1">MLGGNLEILINFNSFRNIDLFDQGYYALKVEMSSENAEVVQPYLVLSQSGDKYEESIQDSQFCSKVFDIQYSEQYIQLDNACLFRILYQAHPNKNTAIKVNVGLLYSQTLEGDTPILSMQQVSNFECIINNACEGVQQGVDVIFDNNHLCTTRMYIYTMILDYRYTGGVNGFQEFLKIKTNDFDQEEVNRFIGEYVDSLGSIQTKYRNLLIQIINELKDKNIQMQNLMRIPIVQTAKFKANLQTKSLGEDCLMIINQLGQSLFQLWNQFQQVLKYSRNYLLECVDQRLLQNCKKLSSESVISNQATFEEIQTQISLPLLKQREEIWNQARKSSKNQIPNIQLLDQFYTLPITLPFFFEDVVSQQQIITQFQQNQQNRKHVLVLVHGYQGTSADLQTWKSYLKIKFPNHLIIQSEINQDDTEDSITVMAQRLAQEIQKQISDRTHQKQQISISFIGHSLGGVLIRCALQHLIKYQECMHTFISLGSPHVGLGIQQSTLIDAGLWFMKAFKKEDQRICLNQMTLCDEKDISKTFFYKLSQNSKFGWFKNVILAFSLQDSYVPFSSASLTRIKEQGERANAHNQMVDQLFQQIPQTLIKTSVFFPNMKTNLDKMIGRAAHIEFIDNSSFVRLFIDYYYEYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NND5</accession>
    <name>A0A8S1NND5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0970027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NND5"/>
    <dbReference type="OMA" id="WQYEAST"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8094777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="23100" checksum="D9B15C1F97AF76BD" modified="2022-10-12" version="1">MSENQETNRDREIREQMKKQYLEEETKLQQGMEEEESALRDYFKNIQKPLPSDPTIIRRKRYNEKMATYQEVIKDISLKEMVKTIWQYEASTPEENETLYDCKQFFKWRCYEGMGACLAMTSLISFFNRRYLSYTAKGLGVFVGLGTGFAMGFLRSTQYTLQRLEELGQDYYLGRLAINEIEDFRMDKVIKKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTA2</accession>
    <name>A0A8S1NTA2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">60S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T1010116</name>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T1010117</name>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T1010118</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTA2"/>
    <dbReference type="OMA" id="XVREYEL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000004">
        <property type="entry name" value="60S ribosomal protein L11, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="11">
        <location>
            <begin position="35"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="12">
        <location>
            <begin position="92"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8096485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8096487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8096489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="22642" checksum="4B4B4F769E595D4B" modified="2022-10-12" version="1">MSQQQPKGGKGQQAKPEAKAQAPAKVVKPQETKENVMRKIRIEKLMVHICAGESGDKLTKAAKVLEDLTGQKPVFGKARYTVRSFGIRRNEKISVFCTIRGDQAKDILFRGLRVKEMELKKRNFSDSGNFGFGIQEHIDLGLKYDPYTGIFGMDFYVVLSRPGLRVAQRKSRNARLGTAQRVSKKEAIEWFKQTFEGNVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWW8</accession>
    <name>A0A8S1NWW8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1000068</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWW8"/>
    <dbReference type="OMA" id="IVIHYEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="388"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="640"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8096050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="770" mass="88949" checksum="C303F36E9EA5580C" modified="2022-10-12" version="1">MGQLLLKNIVNIIERGSQDEKDAIALGLNSCASVSILICYFMIYHLGKQKFIIHLLIQTIIVIHYEIAFYESNTISAQSAIIYMMQIQLYLQKRNGLFDYLSHCALIYAILRFLIREFTNLLVSEILLMFLWQPLNHYLLYLQKSKKPQKKEDITTQVHFQTISSPQVQHTQGLDEIVTKTNMSEDFFDYIPEGLVILDEYFNIIKHNSKILQYLQIKDPQIIPSSLDSLLKIAQKNKVPMKEKDRKRPLHLKSMKQNSEMNFSYTGSLASSLKKQFWTSDRQQLNYSFQKGDSMIYPSLQTIINEFKLKNLNDNITINSEQCGIVKYQITQESSIKYKYISIKIYEIKMPTNSKSTFFLFIVENITNKEQLKQLTNRFIFQQQLLNSFSHELRTPLNCTLSLLQALKSKLKNDEINNDFLNPAIVTSHRLLYQINDILDYAQIECQDFKINICEFSVSDIFQTLKDFFIQECAQKQIQMIIEIDCLIVIRNDKERITQVLINLISNSIKFTPQGGRIVVNVQKRDNLYQFTVWDNGKGISYQTMSNIFQQPNSMVSSNRVTDNQFQSNKLGIGLKVSRGIVKHLSTNGDLIIKSQSGCYTSINFFVEDKFMKIDEDISEQETLMKLASKNTLKKCVCPQILIVDDIPFNHIALIAILNNFNILSESAYDGDQAIEKVNDRIKNSQCCKSYRIIFMDLEMPGKNGFQTSSEIVAILKKVNAQSVIVMHSAYTGEENVEQGRLCGMKEFIPKPISLNTLQNLLNKYFSQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZM2</accession>
    <name>A0A8S1NZM2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0980050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZM2"/>
    <dbReference type="OMA" id="QLSEYVW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8095195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="358" mass="41435" checksum="E22801FE8A897338" modified="2022-10-12" version="1">MDQILFCINEKCTSQSRLLLTMADVPKHNDKSHKVVQFKEFKSYIMEKYGDADLQKIQKITSNEKAIKDAISKIIKEVKEKLDLFEINEIKKMPSLQQLSEYVWTVRKTENLKDITSDLLKTVVEFQVEKIPKYGFILEQIDDRMNKLKTEVIGMLKAQQYDLQITTLGNVKYTWSDQFKANIYAIKDEAKKEIEATTSFMGWSFACIQQQLTSQKRWEAKLEIRASESDQKNLQASQLGQSQLKKQSYSFAIGITLQKLRQLKGLEQQDLATVGHGFYLIRSDGWTCHNSDKNINWKQGLAFTPEPKDVILIKFDPSDGDLVFEQGKNKYQMKVDRISNDVFLFCVHLKGCKAKILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZR9</accession>
    <name>A0A8S1NZR9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0980125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZR9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035902546" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="286"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="42105" checksum="1125C260AF563378" modified="2022-10-12" version="1">MQKFLAFTLTIYLVCSVDPSMVQLSTEKIDELSQSNLGRFIIEMAQTHAEMRGPLDDLVTAIGDLENELTVELQQLEDDFTRSTNQHQITQENLDINIGQTEINIFNQKDFIDAILLPSIDQTNQKIDRLNGFMNDNRDSLARETLNRQNQHQAYLDRVSEHQGAISAVDEALQLINSLINGNIAFTEKATIHQAIKRVNNKITKTSTIHPLVEALLQLTQNFSDQGQAKKIRDLLQDTRNQLVASLNQENTDEKQIEETWVERQKTLNTEYQEFKRSLLEATYVLAAYQNKLKSTQEALAQNELDLQNYNESLQQDKDAQAQETQIYNELKAQYQIQLQTTRNAKDFVNSAEFSTVIRNKLNQGGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P052</accession>
    <name>A0A8S1P052_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1020023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P052"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="36" mass="4236" checksum="D465930576816713" modified="2022-10-12" version="1">MGKLTKVLQSKLLRGRNRENIISKFLTISIHDSYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1U1</accession>
    <name>A0A8S1P1U1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0960156</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1U1"/>
    <dbReference type="OMA" id="NCVNCKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="652"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="758"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="853"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="930"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="958"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="989"/>
            <end position="1009"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8094674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1053" mass="123251" checksum="DB73C2F378D307BA" modified="2022-10-12" version="1">MQEINLFLFQHQGQRLKIDIKVDLLKLHNNLPTYIGMRDFQLFLSNEGCMDKNIVPFDGCFQNNYDCIQGCENCIKGICYGCFIGWQFLEQIKSCIPQCGDAIITYDEECDDGNLLAYDGCYQCKYSCSQNCRVCEFGKCLECGNSYLLLDGQCQYISDYLDNTYMEEQVECFNFFMSQEQYQQYRTQCEYQSIYQTNDDLNLINFRVFGYQHNNQSIFLQINNCKVEQFGKCLECQNEYELDFHKKQCIPKCQDGIKLSQELCDDENRIQFDGCYKCQQSCQIECLFCSENKCYICKEGWQLQEYLCIQICGDGLLAILSIEQCDDPEDLNCVNCKYQCDHDCLVCDKFQNCEQCIYPYEIKEGKCLPICGDNIISLPFEQCDDGNDIPYDGCFECQYQCSYGCIQCEKDNKCVQCEDQYYYLNNQNFKCEQLKQIIDSELKQELNDNDQLIVQCNQNQAFFNNQCINLCGNGILNSHFEQCDDGNNDGGDGCSASCFQEDSFKCLNQENSISICTFIQAPNFNLILLSEKSNKTKIIDISFSQEVYISCENLFDHALDIMIIPQTQYELTIIPLINITSQLSNPIYQINIELLEPVVDPILEISIQKFCIFNQFDLDLSTNSKQLPLGTPIILSTATQKRVISVIKMNDAMVYSSTSISVILLLTGNYIVFFNLLDLLQSLSYIRYMQYKFPPHLTQFLETYTKISLQPILDSLKVDEIIAQINGGTLPYFRKKSQQSKQIDVLNQFYLMNAKGCYFSYFLSLLTYFMCCLISSIKVSQWMGKNFEKNKDSMKVLRIISIFQKRIQSKCSKVKNYYFSQGIYQLFYSTLHQLVFSTLLQFPEYTFNSIFEIINSIVALTSLLFLIQIFFRLLSITTSQIKDKQKWKYFFQDSKIEFWASNFKPFQIYRTVFYITIIVEFIRYPEAQSILLSMQSLFYLIYLIFFRPIKSNFDLIKLLFREVIFLLITGSFLVYSLELNEDQYLLYGWLHIALFCFIMGFTLIVDIVYQGKQAYYTHLKNIQKKEIKQIQLYYDNPLQKFVWNDNLNLKKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P838</accession>
    <name>A0A8S1P838_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1080102</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P838"/>
    <dbReference type="OMA" id="RCCFFGK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00299">
        <property type="entry name" value="GST_C_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050983">
        <property type="entry name" value="GST_Omega/HSP26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="88"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8098968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="28304" checksum="AA56B1003640B9D1" modified="2022-10-12" version="1">MDRRFKHTLVAFKICPYCMKVLTIMCHKNVKFEIKFIEMHNKPDWFLKLSPLERVPILIIGEEVVLFESAAIMEYIDEITPPKLMPDDPIQKAIDRAKFEYSNEIIKNLYSFVFCLEQEKFINLKQWLIKHFKQMDEWLKDKQYINGTEISLVDFNFVPVFVVLNMLKPILPCDMLKDYKRLQNYGDSLTSMQCTKTGRVPDYEFLMIDGIKNKNTVLFRSNPCYFNGPKASRCCFFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8D5</accession>
    <name>A0A8S1P8D5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1090087</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8D5"/>
    <dbReference type="OMA" id="ESCEAAP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000218">
        <property type="entry name" value="actin, alpha sarcomeric/skeletal-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000047">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8099319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="41734" checksum="C451B08CE1847E67" modified="2022-10-12" version="1">MSEEHPAIVIDNGSGQCKAGVAGDDAPRCCFPAIVGRPKHQGAMVGMDSKDVYVGDEAQARRGVLSLKYPIDNGIVNNWDDMERIWHHAFFNELRVSPEDHPTLLTEAPMNPKVNREKMTQIMFETFNVPSFYVQIQAVLSLYASGRTTGIVVDSGDGVTHTVPIFEGYSLPHAIQRIDLAGRACTQYLVSILNELGISFTSSAEMEIARDIKEKLCYVAQDYEAETKTYKESAANNKPYELPDGNVVVIQNQRFRCPELLFKPNFIGLEVPGIHELTFKSIMKSDIDVRKDLYGNIVMSGGTTMFPGIPERLSKELTNLAPSSMKVKVVAPPERKFSVWIGGSILSSLSTFQTMWITRAEYDETGAQVVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P9X8</accession>
    <name>A0A8S1P9X8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutathione transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1100021</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Mu family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P9X8"/>
    <dbReference type="OMA" id="LCYTDFE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050213">
        <property type="entry name" value="GST_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF222">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01205">
        <property type="entry name" value="AMPS.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00363">
        <property type="entry name" value="AMPS_(cytGST):_Alpha-__Mu-__Pi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="3"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="89"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8099548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="218" mass="25877" checksum="33588334B7BBDA0F" modified="2022-10-12" version="1">MENKVVLGYWAIRGLAQPIRFFLEYIELPYEDKRYTDPQEWFGKDDQTFNSQFTNLPYLKDGDHTVFESDAIYYYLAHKVNKPELLGKDAKQQTMVATIRGVVGDFRATFFQWVYGSKETAEAKKEAMLQETGEFFRVFNTTLERSTWLTGDTITYVDFLVWEYVDEFLVLAPEVITSKPKLVEFHNRISEFPQIKKYLENPNYIKRPFNWDLQAVFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PDD0</accession>
    <name>A0A8S1PDD0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1130100</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the citrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PDD0"/>
    <dbReference type="OMA" id="SGVMAHW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06114">
        <property type="entry name" value="EcCS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000002">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024176">
        <property type="entry name" value="Citrate_synthase_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010953">
        <property type="entry name" value="Citrate_synthase_typ-I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01798">
        <property type="entry name" value="cit_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004126">
        <property type="entry name" value="PRK05614.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42871">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42871:SF1">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001369">
        <property type="entry name" value="Citrate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8100791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="480" mass="54810" checksum="634C0D7B1C64D396" modified="2022-10-12" version="1">MSQSPHKWHQETIDSQKSKMEKTVPICPLPQDVATLTLPNGKTVQLPVYKGTKGPPMIDITQLYSKTGYFTLDPGFSSTGACMSTMTYIDGDKGELLYRGYPIETLAKYSSYIEVCYLFIYGHLPSQKELQLFEETMVSEMMINEKLIEFYKGFASDSHPMAIMVGVVGALSAFMHKDFDVNDPRDREYIAIKLVAKMPTLAAYAYRTALGLPIIYPNKKYSFIENFLYMMFSTPMNEFNVDKVIVKALDTIFMLHADHEQNASTSTVRIAGSSLANPFACIAAGITALWGPYHGGANEAVLNMLKEIGNKENIPKYLQKAKSKDNSFRLMGFGHRVYKNYDPRAKVMQEMCYNVLEKTKRANDILELAIQLEQTALKDDYFIQRKLYPNVDFYTGIVYEALSIPTSMFTVMFAVQRSIGWICQWMEMMSEKVQRISRPRQLYVGEDQREYIPIKDRKEEEKSRVCKLPMHSSLFGLVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGH5</accession>
    <name>A0A8S1PGH5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">VIT family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1170053</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8101951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGH5"/>
    <dbReference type="OMA" id="QMCCVGG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8101951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="28610" checksum="A356D5B2593E8029" modified="2022-10-12" version="1">MNQAQIAFEKKDADLSKKAHSIEEQQQLNEGHEEKHQSGGEHIKSAVYGGLDGMVTTFSVVAGVAGAGLSTGVILGMGIANLIGDGISMALGDYISTRSEAEFTINERNREQWEVETNPEAEKKEMIDIYKSKGIDHDEAVIIADTLAKNKKVWVDVMMVEELGLMSVDEHPIKDAIVTFFSFGLFGLMPLLPFIVGSIAGLSENLFETSIALTGFFLFILGVSKSMFSYQKWYWAGLETLIIGSAAASASFIIGLAFEDVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJW3</accession>
    <name>A0A8S1PJW3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1210040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PJW3"/>
    <dbReference type="OMA" id="TFKNIWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="712" mass="83489" checksum="C84E3A46E9241715" modified="2022-10-12" version="1">MRNRILSKDDDNVKEYFSTQKRKQDNNIQFWQGYMSIALNSTEAGVRQFLTQMRRNGSFQQIDKKQKPVITEQEQLLETPNHSIVKSLDPQFPPFIRKAKEITSYEFLSQIYELQNRKTRNQSLIEQLIGKTNNDVIVFPKTIHRKNIQKRSQRCQSVAQKDIELDSMKGQLIQVRQVQVQPIPKETTPKPLKRLIPIIKLERTTSPIKKAQAPQSQTKKKMFIDDTRYWDLGYIKFIQCPSVQEINQIINFTNALQVLPAQHQKYYKLYVGRGNNHMMVKSIFSMRAQWTVGGNLEEDDFNFIWTQKYVDFPQSEIRPIQRSIQNETIEGWVDSNDQALIRQAWDRVEGKSKKKLADYNLESQPLLNNLSNVKELLTINHQNQLIRIHNHLKEGNQLGDKKFLFLNLSKYCKENQIDLWTLIPLTYHIQGPNDESFILFKKKFEEVSDDSTFKNIWIIKPGEDSNRGQGIKVCNNINEIYQHISQQNHTFILQKYIENPFLYQKRKFDIRGYCLITIINGAKKGSDHINKQIVFWYKKGYLRTSSSLFSLDSLDNQKIHLTNDAIQNKMNGYGKFEKGNKVSYEQFQNYLLEQNKQNNTNYSFEELYKNMKQLTKIACSSSINQINNNDQLLGFELYGLDFMITNDFKPILIEFNTNPCIETGCPVLAKIISGLLDNLFRFIIDPLFPAKKTNLDDFNSKNDFELLLQSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PK27</accession>
    <name>A0A8S1PK27_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1210079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CANIGCR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="26"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035935371" evidence="1">
        <location>
            <begin position="17"/>
            <end position="2182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2182" mass="238512" checksum="E5D8BA9AD0B5E67A" modified="2022-10-12" version="1">MLRLNLLVILASQYQGAQINVEESCTCSQIKSKFDCANIGCRWDIDKCVQNDQIENEDTISVYCRQFDQATCPKMKGCAFVDNKCEQFSGCSAYLGSSNDFCQQISQQCTSDGNVCLDPQNCNEYKNQIFCNQNINVQGGQCKWENNQCRDQRCTEASNSLKTDQECNQFKIGCVTNGKGCVEIRSQCSSYGIDDCNSMIGSEGYCIRTSSGCGVRECQSASSDYNKDEQCMRYQQGCVTTGKGCWMKPLPTCDSYVGNDCQYMIGSDGQCENGSSNKCQSRKCENAPSTYNTDDKCNEYLKQCITNGRGCDTSLKLCSSYTGTSEECNQYIGSDGKCTLGSNGCKVRVCEEAKLKTDRECNEYQKGCVTDGVNCLSNRKLCSTYNGTAATCSKYEGSDGRCYSKSNVDGSCSNLLCTDAPTEYKTHEQCISFQSDCLSNGEGCVKKTDCVSTIKELTCKATASCQWNQICVTRTNCSFFKSISLCNNNLANGQSCFWVNGVCRIRLCSDAPLSYTKDDQCKKFLVGCVTNSQGCLSYTAPCNQYEGTIDTCINFKGNGMKCTSTSNLKGPCEEMLCTNNTTALNQKQCDDFLNGCKFQGNSGCIDKSADCNQYTGNQERCSSYFGINGTIKCYQDVGITGPCRNLQCADNITATSNEQCISFLSTCVSKGIGCIDQAEPCSSYAGNNISDCSKFKGFNNTKQCWWISGSNCVNKECIQDITSTSNEQCEQFLSGCVTKGIGCIENTQPCTQFQGDETECQNFIGNGYPCVRKSSCMNRSCSDVINPANNDQCVEYLSTCRFNGFICIDAQISCSSYIGLNYQICQQITTISGGKCYLANGTGTCQTRSCTQLTIATSQNDCDLFLQGCIFSGSNCVDQQNLCSQYVNFQPNACEKAVSISTGLCWRQSNSIGTCMARTCLGVTSTLNPYSFSSQFCTQYSDTCVYDGVKCVNKQSSCNSYTNFVQSACKIAVTLSGENCWLENSALTTCESRQCTNTVTTPNSINCVAHKSSCRYNGTKCEDAQAACNLYTLFNQKACKDVTLIDGVTKCWRSSNSVGTCEDRQCSNITVATTPQKCLDHMNTCRFNGSSCVIQQTNCNGYLGFTSEACKLVTTSSGGLCWIPSIGSTQCSNRSCSNTIENASAITCPQHMSSCRFNGIECVDHQITCNLYTGFTKIACQNTTTISGIKCWKQINEVGSCEERTCSNLIDNANYVNCGIHLSTCTYDGKNCYTMQNSCNLYIGVSEFQCQNLRTTTGDRCWFVSGTCTQRICSQNQIGMTDLECDSFLPGCRTNGKGCVDSSLSCSQYRGTSSSCKTFIGNGIKCKGQDSFGYCEQKQCYDDMNSVTDIQCDQFMKGCVTRGLGCIDKNEICNNYIGNQLTCSKFIGNGKNCWNDNSSTTTISQCRVRLCSDNQTYNTDSQCQEFQVGCVTKGRGCIDKTAKCSDYQGTQIECSQFIGSNGTKPCWNVISATPLMNCIERNCQHNITAKTDNECNQFLKGCVTKGIGCVLPQPCSLFNGTIKSCPLFSATDKPCKGQSNTTVTPCAALQCNEAPNNYNSDELCNIFKEGCVTNGYGCVSNVSCEDIQTEQACQSKKQCLYIGNCTQLSTKCSVFQSQSVCVNTPVYTDTRRCSWEFNKISNLGYCRDWKCEDASESVLTHEDCQRLSNQCTTKGKGCIPIGECSSYTNSNACSSAKTTDEGGLCIWDKTYCRKLDCGDASKEFTTDSKCQEFLSKCYSNGKGCINQNYSCKDIVVKSKCTKDYAGNICLWFQQQCINFSQCIEIQGSSHDQCQQYSNKCTSNGLTCIPISQCSKYPNQISCSLGIDGQCGWIDNTCAPFKKCSDLNAINTELCQQYSSLCVSDGQGCIQKTECNYYLTEISCLAGGTDGFCIWAENSCRQRKCTDATISMDIDITSYSSCSSFIASTKCTTNGTNCISIGLCSSYKEQGCHYGTDGQCIYTFQEGQTQGIKQCRVKSCSDYQGTTTEICKQHKKVCISNGNNCIMKNKCQTYKTKTACNSGGLDGICVFTPSVADPQKGTCALMTTCEQANSDLIACKSKQNSCHFQVSLQNGLEVTSCINHTCATVANGTICKPVYQFDEKTITVCVMTSSGCSSGNPNQLSASNCLEQSLNTYTWNTETNLCQKCNTTSISPPTPTNSTETSSDTFSKVLLFSIFVFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLB4</accession>
    <name>A0A8S1PLB4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1210126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLB4"/>
    <dbReference type="OMA" id="RQLEFNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="58837" checksum="AEA08D591762C9BC" modified="2022-10-12" version="1">MNKYSLIFLDKESERKYQEQKINQLRKPVYICSAIGLFFISMIKMITEILDNQQNELSIIIVTQIYLIVSIFIIKWKPQLVQSCLIILNYLLLAYQYMLTNTISQEALQLFANNFVACNFVIILTVDMCQGIILVTTTMIIRMSLAISEATTPQYNVYFATILLICFLSFFIHKLNYESRSYFLLSLKDNLMEQILPIIVNKSYILFQFENNQLRFSLISSRQLEFNCDNSENLKSFLREWNYKQQSLEQFCFQDIKRKQSLEIKSYSKQIEIKKGSKQTQKITICFFSYQQPTFLIKFDNNSQIQDKLKNYLFKSIEKERLVQFKIFKCIVNNLSVSLRSSNLQQLHLLRIYCFKQILNYKILNHQFKQQDFEINPILQKLKDFFKFKQVNYQIIPSSDGKVQMVKTYFLRLIYDIFNECKEDSTIVINLEHRLEPYIRYYGREINEYTDTVIQTVVKTLVKKQQMMKIGNQTTILILLNEEPNYPFQSSLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLY7</accession>
    <name>A0A8S1PLY7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1220145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8104009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLY7"/>
    <dbReference type="OMA" id="HKSTIGK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="70" mass="8182" checksum="4DCD37C4C7D7E8E5" modified="2022-10-12" version="1">MSFTPPKSLSKTSYIQRVPFADKFQKTFSDAEMQCVQRKLDFGDMDLQQKKSISTFRIHKIHKSTIGKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMW0</accession>
    <name>A0A8S1PMW0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1230125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMW0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="1">
        <location>
            <begin position="14"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="12186" checksum="B9623E1F6BF5808A" modified="2022-10-12" version="1">MDQSKKTLVNPETNVYNLSGKTLPKTSVFVAKIYLKRFDVVEVHALGETISKAVRMAGQLQRQNYVTIEKINTFTQKFDDDRSKAKIVITLKVTADGKKRVIEEIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PP71</accession>
    <name>A0A8S1PP71_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1260144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PP71"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="270" mass="31675" checksum="392864B1915D9DDF" modified="2022-10-12" version="1">MIVFQNKYIILSDSKGSITRISDTNQGKKIESWLIDRSGLRTVISNRNQDTLFTVDNKACIYVIKLLNKKWKISQKIQVKNQIRQINLNNYEGGLIVLGSDGYAWLYTLNRKDQCWEQTYEFFPFFVCLKMCVRGPPPLDQIVFVEPNTIILLRLEYHLYYVDNQGNAEFQELIDLQPEDYNLRKQQQIFCPCKLNDNLELFRININDRQLLIVRDRLKGTLTHAKQNIDFQNQSCLQITDKGEDLLYSDETGFYLITIRASKEIVALIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PW40</accession>
    <name>A0A8S1PW40_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1330107</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PW40"/>
    <dbReference type="OMA" id="VCIKDPR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF874">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8107316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="356" mass="40610" checksum="CFDBC05CC9A1F02F" modified="2022-10-12" version="1">MFTTRLAYRFAKSVPVVDVTSFLAKKGDSSAQCAQVAEALHNYGAVCIKDPRVNDRHNVDFLDMMEKYFESRAAKFYNNEKVEDIFPNYSYQVGATPEFVEKARTHTNVIRQYTKENEAYTPQPAPYDAKWRFFWPIDDSGKRNKDEDFKPPRFRPKDVPQFGERMEQWGKMMINGCLTVAEMAAIGMSIPADTFTTRMQGGPHLLAPTGSDLDRHKLGTVFAGFHYDLNFLTIHGKSRYPGLYVWLRNGERISVAVPEGHLLLQAGAQFDLLTGGYVTCGYHEVIYTEDAKKRYEENKKAGKSTWRVSSTLFSHINSEVTLQPLGKFAKQGLPEKYKPIKAFDQVQQELEAIKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZF2</accession>
    <name>A0A8S1PZF2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1350074</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZF2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000457">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8107898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="440" mass="50696" checksum="AAC69BA64FC9B6D3" modified="2022-10-12" version="1">MNNLSFDDILSKKIKFGKVQIQTFLIISLINLVDGSEFMFLQLMNAIIYKEWSLSLGQLIVLTTIFNLGQFIGAMFYGQFSDKTGPMLLTAFVQDLPQLLILRFLFGFLFGITSPVSSILMAEVTPLHIRGQFIVALQIMCIVGRMWMLLLAIIFLDSIATGNWRALAITNSIPCLICLIGSIIYIHESPRFLISQGYIKEGVEGINFMGRMNDKDYIDLQDDEIQSLQQWRKSIFEQQFEKKSFKDLFNQENLPITWRAYLLSIIAMFMQSGLYIIIPFLFNEEEKTLLDLFYTVLIEMPAVLLVVCLIDRIGRLSIILIGTITSMITVFIIWYWKARFLLIGLIIFKFFNRMIFISFTPLILESYSTLYRSLGTGTTIAFGSATGFFSPAIILHLYQKDNYSPFLVSFFILIMMTIIFTTYPKDLTRKPLDIKFQKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZL8</accession>
    <name>A0A8S1PZL8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T1350116</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZL8"/>
    <dbReference type="OMA" id="IDEHERY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000064">
        <property type="entry name" value="Protein phosphatase 2C 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="16">
        <location>
            <begin position="23"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8107983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="300" mass="33582" checksum="F402A9F4DBB46F81" modified="2022-10-12" version="1">MGPYLSQPNRNKTTTTGSGKSVIFAASEMQGWRNTMEDAHIHRPDIAQDVSVFGVFDGHGGREVAYFVEKHFIDELLKNQNFKDQKFADALQETFLKMDELLMTPEGYKELNNYKSNNSDDSYAGCTANVALIHKNTLYVANAGDSRSVLCRNNTNFDMSVDHKPDNYLEKQRIERAIGFVSGARINANLNLSRAIGDLEYKRNSNLRPHEQMITAFPDIKIIDLGQQDKFILMGCDGVFETLSHQELLNHVNATLGNASVTEKLLQKAAEDLLDQLLAPDTSLGLGCDNMTIILVYLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1A5</accession>
    <name>A0A8S1Q1A5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1400064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="96"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8109280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="286" mass="33959" checksum="C308FF997BDF2BEA" modified="2022-10-12" version="1">MGLCNGKTNSSKMNKSQSQSDKIQNADPQQQTTSDSQFQRNKRYGHLLQPNSAQHKLTMILESYQMQKEKLISSPISIRSHQTLLISKRNAELNKLLDNDFIPQEEMNQAEKCFYKYFVNKFTGRIYYTNNSDQGEENNHYSNIKINRAALCHPHIIEILEIENEESQQKDKLFFEYCSQESLQDFIDKNQDFNLEQIQIIASQIISVVGHLHSQKLYHNNLDMQSFHFFVXYVFLKLDCSIIDTNYNYQIFNKMNKVIYFQLEYQLKLNNLHYLLNYFEIKNIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1F6</accession>
    <name>A0A8S1Q1F6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1370159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1F6"/>
    <dbReference type="OMA" id="CHCIERR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8108638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="69" mass="8156" checksum="1BE019892D45894C" modified="2022-10-12" version="1">MSENQDNKIELYCWISSQILIVVLAIILGCCHYIERRKFKNKLLDFSDIRDTHQYKYNQIDPILAPLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5G1</accession>
    <name>A0A8S1Q5G1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1440078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5G1"/>
    <dbReference type="OMA" id="QKQMNRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="259"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="323"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8110477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="559" mass="68449" checksum="0BB466E8DFFBF888" modified="2022-10-12" version="1">MSNEIILETVRELLPDIEKEIREQVSQELQIEYQIKFQQELDELTQFNEEQIRDELEKQIRIDYEQQKEQLAQDVIREFDDSLQRGQLEQQLRFKMQEELEEQINEEVQRKSKQIRLQYKKKLEQEKNQLQKDFELEWKQRVDAEKKRLEREKSEIARLKSVEYVKLKKLQDEQKKHSQLLKDQETKYQDIMKSLQKQIQELTQQLEIALQDQIIQEKLRSQQENFDSIDQDKQINIPLQKQDQIYNHKSNPIYQHKQITEDESQESSYTQVNLHKQKQKNNIQKSDQQEQNNQQKNQNKKQRTQYQNQLEKGVKQQKRHGETSQPQYLEQENNQKQMNRIDTIIKQYDEIPAQKEQFMSYNSLFKENDLDNSKNKQMKQRRQKFVENQDQDQRLQTKYLGIQINIMNDFIVDYVEFSEYHQVKMMLQEIIELFNQWMISYQERIEFFKRVKQIANSNFRDIIPFIQTQIHKFKLIQNYYPDFQKRFNLKNQIMANYSQFSKCKHLFDQLNQLNLKSINISVLGCDFQEMLQLDQWEKDFLEREELKSQILCKIQSKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QE92</accession>
    <name>A0A8S1QE92_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1580045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000163">
        <property type="protein sequence ID" value="CAD8114138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QE92"/>
    <dbReference type="OMA" id="HQQQNKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36851:SF1">
        <property type="entry name" value="GLYCO_TRANS_2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36851">
        <property type="entry name" value="UNNAMED PRODUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="420"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="464"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="61395" checksum="92842C0BCF047940" modified="2022-10-12" version="1">MNQATNNQRLVFSSELPDNPMATKMTQKEDKTCGGRFRWNLIINVVMVAMAGFPFYLPLAATLGVNCFYASLWILFMFLAANTQSKIHLTMKKVSNQSEQPPNPTPIKFILMTFIYKEPLELLLKTLQNIQQQRIAKEIIMVVCMEEKTPEKDTKINSVFEQFKDCFDQLIITVHPYGTPGEIPGKCSNNNYAIRSVLAHLKKNEPNLDPNKYFVTNFDVDTIFHKNFLDVQMMNILKEKDRNNFVWQPVLFYNWGLDKLSFFTRITGLARNMLMMGALIPLNINIMSVYTSSLQLQIEGDFCHPTYQMDDIICYIRWLTLSRRSLKIKPIYCPTISGPTSGSNMWQEFIEWVRQNKRWSVGSAEVFHYFVIKAPRIKFCSAFIWACNYLNYYASFICVQSLLLITTTIRLFVMETDPKLQLYFCIPLIVVYICLFFMMVMNKIAVKYLLKEIVVENIPIWKDFIHWILSLIVMVGYGLTVFYGFWEIFFCGKGVCTHEPSKKKLLDNIVQRKDDTVIAINEQQNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF27</accession>
    <name>A0A8S1QF27_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1560022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF27"/>
    <dbReference type="OMA" id="HTKTIFR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="332" mass="38953" checksum="8DA32047BC54B56F" modified="2022-10-12" version="1">MDLLNLVQLAIDISLTRDNYCEEIKKLRINLEVFCHTLKQLDDSNCPNQFLKEAEQEIQKTYEWLKNLPQEVSILDSVKFQIPYNNKVEQIKEKCNSIQQLITLSTALNVIKKSTSQIPSIPKPPQTIQPTVTQVDDIQINEKPEKYIVLSVISDEHTKTIFRKHGVNLQNKHAINFKENESEIVICKISRQDYSLLKEDIQQINTISQDHAQILCRKIDRKQRYPEIKIDENNDQQDVFYSLDDNVKELSKNINDELKYEFYLKITGRLGVFLYRKKENITDFGKQIIPDTWANRIQNTTIKLEENDKFAILMKKPDYKVPLIAYSITYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QGP7</accession>
    <name>A0A8S1QGP7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1610096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000166">
        <property type="protein sequence ID" value="CAD8114832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QGP7"/>
    <dbReference type="OMA" id="SKQTNQC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="145" mass="16913" checksum="3B83103239D25278" modified="2022-10-12" version="1">MGCSMVFIQKASFDSNSSFSYDDIQESEVSLDQIHDGIAFLKINVKKQKEIFPEDNNTEVKHLVNCNHHAVIQINKSDIKSKKSCLKQKLITSQLSQNVSPKIVHFVQIQQQNTNQYKRRQKKSKQKHSRKSKERQEHINLDDIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJC4</accession>
    <name>A0A8S1QJC4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1600053</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJC4"/>
    <dbReference type="OMA" id="NDEMWIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000217">
        <property type="entry name" value="Chromosome 16, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000201">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035945839" description="Peptidase A1 domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="7">
        <location>
            <begin position="22"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="402"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8114525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="414" mass="48249" checksum="EC93E78F644676D7" modified="2022-10-12" version="1">MIILIISIVTCLQINMTNLQDNYYSLPIQANNITYNLVVDTGIAETWIFGKNSNRKKYYECQKCIPKNKEIQEYAQGKISGIEYNQKIEIMNQSLYLNIIETTKVEHFKLIIADGAIGFSKLQLSKENNLLKNLYDLKVIDEIQFGLLLNEQNCQSTSILILGKPIKNYYINELYYIKILDSSLWMIQGKSVEIVGKNNKLELESSSQIIVFDSGLSKILISKQKFNKLIDILNENYNLNCQQETSYQINQIVCSYNEQQYPEVNINIDTNFSLTLLPQDYIEYCYYNYFLQHKCLLNFQQIDNTDVLVLGIVFLQKYYIHYDITMLQIGIAQSIYYQRHNQIKYNSNNSMLYFSLILVLALLIAIYILLRMFLKKSIHLYLSVLEKSSSRSQEQEMEAITLKTQSNNQPPLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QN07</accession>
    <name>A0A8S1QN07_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1730023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000182">
        <property type="protein sequence ID" value="CAD8116491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QN07"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="115" mass="13819" checksum="AA1AB8A8A1CEB4CE" modified="2022-10-12" version="1">MRLKNYRYNVLLKNMIVSCNLHALIKHAKKIEFIVSIFLIQKINMIQINLLNFLIKFRKDCEDLIERLCLEIGNIKKILIQLNQGLKIKYQLQKQRIFNLDAKQLNQTFDEMIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPL6</accession>
    <name>A0A8S1QPL6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1930005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000202">
        <property type="protein sequence ID" value="CAD8117251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPL6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="151" mass="18436" checksum="9568DCE054AA52A0" modified="2022-10-12" version="1">MEQQAICYKLCQQIDLTYKSRFSQDQLIEKRIKLNLQFQVQFKQSELTDNINQMVTQDQILMIKGREDIVSINFLMLKTQRYIERPQSQKQIRATMQMESYQIFILPRRIITVLGLFKIQQRQQDYQNCTNLSKKILKYLQKKEQYFYQNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QSJ7</accession>
    <name>A0A8S1QSJ7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2260003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000235">
        <property type="protein sequence ID" value="CAD8118132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QSJ7"/>
    <dbReference type="OMA" id="HISCLIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="49418" checksum="DC64364D72233169" modified="2022-10-12" version="1">MSRKEMLNRELISHMIEFEKQLDSSFDKINNLIDQLFIIDTPICKFSNPKFEQSSPKFDQFNLIGLNEFQLDILQEVKPLINQKIKDQVQFKLLQLQQNECILIQQSSQQKEKQQIIKSPSSQFNLKPFTYQIIQHNSIQEQQSCHVIEINKDCSIVAIACQIQIRIYEFKQGIMKQIQVFNQSIYPVCNLKFMKKSNELISADAIGSIVIWSGNKNTQWNSLQTIKGHISCLIVNNNEDLFITSSLDKTIKFWIKKNEWIWQQTITDHIDNVYSLSLNDQEDKVISCGLDVDCFGYQLSFINNNLFAFQPMNGNLMYVYEMNSVSKQFTKTKDIIVNSSHDNYYTLFPSQFIKQKQILVIKHGEYVNFIRKTEKDEFKVEQSIHFYTNILFGRLSDDGEYLITWDDSSKEIQIRKYKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QST9</accession>
    <name>A0A8S1QST9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2190005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000228">
        <property type="protein sequence ID" value="CAD8117985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="245" mass="30369" checksum="744F71054B5A68FF" modified="2022-10-12" version="1" fragment="single">MKRIKNQIYDNYFYNIYFFRVPGQNRIWHHLKIKNLNLFQSATHIFQIVIFLEGNEEDQTKFKHINTQIIKPFFQNPQQEEPLQQEFQIPWKKRKNTRKANVLIISLILLFYHQKNYSFYEKYAKNELLISFLNVLTQFIRMNKLLLLRGLDLIQKLKSLNYVRKWISMKRWMFKFILFKLIKFKIIYLKSIKKEYHFQLEYDITNQIQNNLKIKQMKWKQNLNVIECLQFQLDIIIYQIMKQTX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JND2</accession>
    <name>A0A8S1JND2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JND2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="74" mass="8164" checksum="E570A7B2FE9C383B" modified="2022-10-12" version="1">MARQSKTQKKAPKTATKKIAKKPAGPRQTRTIKAAVAEAQATTQPSAGQRKLPRQNRRNKQAKKAQSKKATQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNX0</accession>
    <name>A0A8S1JNX0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GPR180/TMEM145 transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0060036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8043954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNX0"/>
    <dbReference type="OMA" id="YGEWDYA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047831">
        <property type="entry name" value="GPR180/TMEM145"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019336">
        <property type="entry name" value="GPR180/TMEM145_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23252">
        <property type="entry name" value="INTIMAL THICKNESS RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23252:SF24">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10192">
        <property type="entry name" value="GPR180-TMEM145_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="GPR180/TMEM145 transmembrane" evidence="2">
        <location>
            <begin position="174"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="419" mass="48769" checksum="DC4194FFE6F97871" modified="2022-10-12" version="1">MFLIVLIILALTEAKSITQTFTMKQLQKQQYIPINRFGSKGTVNYKISSRIIKPPKEYENKQFDIAFEFFQQDSWQSALKKDECSRYEDANTIVHQTIEANLDQKVSTQEGQLKFGQYHNIWLAVFNNCQHSIDKIIKINNKNTKLEITVWFSEEGGQEFSLEEQGLLNVVTILTVFTIIGFIYNYRIFNSDLQKYGEWDYAQLFILVVIGLEALQNLLNFMHLIVYYINGKGIGAFSTISEIIQVVDNFLLMILLILLAWGWSIEFMDMEDWDIYVPVAFLIAFAQALIVGLGRLVSNQTDNHLYEGWVGYTISLIYISLAIYFYYSTNQRKKKGDSVDYFYIQLKIYGMLFFLAFPFLLFVSKLVDPYKSYTIISYGNMFVRMLNIVLMARLFTGKSSDYQKICMKGRSFLDRGKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQN1</accession>
    <name>A0A8S1JQN1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQN1"/>
    <dbReference type="OMA" id="IHNQDIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106">
        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106:SF111">
        <property type="entry name" value="MACRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="688"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1061" mass="124364" checksum="2CF0910AD98D1445" modified="2022-10-12" version="1">MLSLIQGDIFQQQVDAIVLPSDIALLKATGLQQLHYQLQQQCNEFKTNQTILSQIQQTSVITLSINHNQFKYIIHCIVPKQDINTKELSMSLLLEILFQNIFYEISFQKIKSILIPVLGCDNADFSIIEFLNTFKLIYEDQRQNFIDVKFILISQTQQEYELIKKYFQSKNPNTLTINDIYQTIPQGNSTSITEKFKQYIQQNIKNNEIQKSNFSDESEIAQYRKEYNANLSKKQQQNYQNQGNSLNQNINQNNIHNQSENLKQQQIDRLQQSNQSIIISSTPLLQSNTMFLTLFIQIDNIILEQKDLIKFMLQVCYDLYKLHQQLFLNIENVEQSNQLFNYKLYYNQSPNRNELQPYCPPEMSENQYISETHDSYSIGKFFKMVLEINYKADQENLQEINQIQQLLDKLISQANQRPQIDDIFNYLLSLQLDESFIENWTQVQKNINYFYRKRGNLQKIQDIKKQMLMKISPVHLNNYKKEDQNQDLNQLLNHQDSNLSLFTHIQQIKFKQDCEQAQREQNYKFCQIDCVVIPVDKYQFKQSLPTFIKLDSKLDKVLDKIVNLAQKDKEHYLQSIRSYVPIFGCNSILLVFCPNQNLTVERQIQLTNCFSDILLYTLKCQKINSFCLCLDEISKKLIVNQNNLSMNILIKAFEETCNKSPDPIIWNEIQFNNQKWIVVKKSNQNNQYLDPLKFTLLDQSIIIHNQDITQIKGIQAIVNVTDPNLSNQGGICKAIFQAAGENLLVQEIKMMFQKLGKKQLDTSEVVVTKSYNLGLENGPKYIFHTVGPKYNSQDPQKSIEQLSKCIFNILQKCQEYKISSIAIPPISARNCDFPKYQCAQIFYSAFLKFKFKNPMAIHIVDVIDNIVEIFQIIFKGEKLRQTIHSKIDFTPPTDCLVCPIDINNYNSQALKRIIELAGPSFQNELEYQIKNYQKKDDQKQCQSIILQGHKMKFNNIYQILMISKPLNIIDKQTLLYEVFFQILVETFLKKKFTSITILFYSILQQFLNDQDDLIRNGGEQAINIFQKVIQNFQEKYEQECGKITILSNVAQFCTDCELIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQU1</accession>
    <name>A0A8S1JQU1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQU1"/>
    <dbReference type="OMA" id="RELDDLW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="31"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="178"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="345" mass="41921" checksum="E1599F6FF2ACC51B" modified="2022-10-12" version="1">MHFNNHNSKTSDAESEIQKWKDRALKLESGFKELQQLMEETDDMRNNLEKKIMENQQLVIENSRLKERLQNIDVSDKVFSNKLFDDHDIKEKEFIIQKLEQEIIDKDNQILKFKREIESINRRSKLKEEELHRQYQAKLEDLQILYKNDTSDSQQQKFLMTKKMAEMEQILSFRTEENQLLINELNLKNEQIVELNNHNKLIVELKNKEFKDSQIQYQQQTQQHIKQMEESIIQYKKSQDSNLSEIEQLLQFKQQMTLKINDLSSTNQHLLIENESLKSTLLLFQKQKDQDDYMYEDQKKKESLHIKRYYETLLEQVEKENRSLRLLLEQKSRELDDLWNQSKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSQ1</accession>
    <name>A0A8S1JSQ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0050304</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSQ1"/>
    <dbReference type="OMA" id="QQKDHIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF1">
        <property type="entry name" value="RAS-RELATED GTP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="37173" checksum="4C2A2BA56059CC22" modified="2022-10-12" version="1">MLKNSENQLKKLLLMGQQKAGKSSMHKVIFACAPPKDLISIGVTTNVSQIQVRFMGNLRIDLLDIPGQPKLLKEYLTDFKDTIFCNVEVLVYLFDVEMEGEQFNSEMITFRSTLSNLSQYSPGSKVFVLINKFDKIKETERKMVFERKYKKIIQEAEGFNIEVKDVFATCIWDETLYKAWSQIVQNLLPDRDIIHNSLKSFCHQCNCDEVVLFEKQSYLVIDFVDVNEKKDILKYERISNIIKQFKLSCKMASIDIKKIMVKSQKFHVQIEEFTENTFILLLFTDEKVYPAAIEYNIQYAQKLFKSINGSETDKLKLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVD8</accession>
    <name>A0A8S1JVD8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0060514</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVD8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="729" mass="85310" checksum="F456DC66B6C1C31B" modified="2022-10-12" version="1">MSEELKDTFDGYLMFDFSNFHGLMAQTFDNEEIDIPRVEKQPKRKAYNIPGPYLDSPPIEFERSGDCSEGILFNGQLGEDDIPEVNLDEDCLSNFNYSTSGFNLLANLDFEPDEDAYDNAINATSEPNSLHHRNSKISRTKKISIFSPTLQFDDNVDQKIQMITLQNMELLKKEDHYDIDDFYKFLTSLDFLNVSNQKHNDDLMKVDQLFHLVQIRQFFDKMVVILFQKTDQSHSISYTSKLDSFKEKMDHFINLVQYMVDDGTYRQKSLRDQHVFKKDYTLTMSVAFFKGLEIISLYKSLLMQKNMQDRLKRYTTAKKNLHSYWRLWGLIKEFEISFVQKNIQKSPNLTNSTLGNQKESVELAILDPHAKIQIARNKDNNNKISLQGFDKYLNKPSLKNTNYCFAGKWNQKKLQTKQLNRLIGLLNYDISLLHKLIFSFQMAFTFGTASYFLNKSSNIEIDFNCYCEKHELQSMVTECTKFFVRKANIQPQIAFQEFVQCYIKGETVLLRPYQQICNSKMNRLVFYASKTQNNQIFESFEDKSGFDYFQLTKLLCSDFPRDIVVSSLSLYANLDEELLFKKTFTLLQFCKALLFELLFENILQELNTKLNNNGIIDSILPILKKYYPNNDEQLYLPNPAAIFECLMKFSNEKNKACLHPILPFTVDMFSTQYLNGGIVEFSSLKQQLFKNTKVDVQLTKLINLIENSNQRIQDCSRLDEDENKKKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZ41</accession>
    <name>A0A8S1JZ41_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KIF-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0120245</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KIF-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZ41"/>
    <dbReference type="OMA" id="TIMSECA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022083">
        <property type="entry name" value="KBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46321:SF1">
        <property type="entry name" value="KIF-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46321">
        <property type="entry name" value="KIF1-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12309">
        <property type="entry name" value="KBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8047936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="519" mass="60675" checksum="A47162912E6CD088" modified="2022-10-12" version="1">MLYEFESTLFQVEHLEKFPSIEEQPFLYKYRARTLLQQLLQKKQDPLQQAIIHYQLGVNFLDCEENGESRKNMAKSLQLFSQIAEIENYLTYIQSIYNHLGFLAVNLDDNETGLAILAKAERLGEYLIAKGDFEKRAFKIQGGYEKAQEFYTITLFYIAQAYTKLGIKDKAAYYCGQTMLRQFKSKTYELRDLVINCISLSEYYSGIWNFAQAFYLLLVGEAVIPEGKRKKLRATLQMAQGKIIGDMMAYCVDCIKSNQQNDPSLPKLFNKYSITFDSVNVKQLVFQPSKTFDEIKSLFRQSNTLYKKAMSIFVLDGFVTEHIQMLSEISNMYKVIMSLETDPNNIIAYLEKRLDLLLPVFNAINPKAYQNTYEKLLVEIAEINNELYEFIVKFNEIDDFQGGKSKKLTEKMNKSCLNTIQYYEKIIEQLKLLEEKQRDSAYYSSITTAYFNIAKAQSKYFSKDKKIKVTYLVNSLNQYKQLVQYINQQNQQNEFQAVLQMCKEMIQILPSKIDRINYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZH3</accession>
    <name>A0A8S1JZH3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZH3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="13641" checksum="70F5E2D321FCC2DC" modified="2022-10-12" version="1">MRYINSVNIVQNLNLQKLLNNPTIDNLQIIYSKLQQLQYFNSFIEKNLQGTQRESLLYLCGKFRMEHFKGGELIFKEGDKSNDKLYIIFNGKVVLMKARPQTFSNNLQILKILLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K047</accession>
    <name>A0A8S1K047_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="641"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="641"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="664"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="680"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1059" mass="123197" checksum="6808CA90F0807CEA" modified="2022-10-12" version="1">MKMERALILNGKPLIPPPLTIEEQLNERLDRTGYKIDDLNRLNEKPVLQIIDLDGLTSGYLNKNPLFQMMKQEIMNWNEKITLYRAEQIKLQDPLVSDNLYRQLKNSLLEVVSTRNKIQQQDFLNKVSNWFFSQLPKSQTASLKQSLLEKKDSTLHFIPNTYNENKYASGIEDYALKFRSVHPEFDPPEDRVKTYQRKNLLSDTSTRPGTTPGVLGISQFTRPQTSQTTYKMIDQVEKLQRPKTNQQYTIPKQQQSWMYDPNFPVLQTNDDQQQKSTRRQIQREESPLKADKPTDRDKLEQIEGIEQEQEEDEDQSPDKLEESNKNLASQQEIAPIKKIKKKGRSDKYISIAPTNKTYKTDIETQNAYQIGQKDRLGEFQGTFHNYDPTQIEDKMVAARLNQYYTEQRVKEVKEQREDIEMVQSMKDWASNKSRIDEMVLQSEYVYLNYXDLDGLTSGYLNKNPLFQMMKQEIMNWNEKITLYRAEQIKLQDPLVSDNLYRQLKNSLLEVVSTRNKIQQQDFLNKVSNWFFSQLPKSQTASLKQSLLEKKDSTLHFIPNTYNENKYASGIEDYALKFRSVHPEFDPPEDRVKTYQRKNLLSDTSTRPGTTPGVLGISQFTRPQTSQTTYKMIDQVEKLQRPKTNQQYTIPKQQQSWMYDPNFPVLQTNDDQQQKSTRRQIQREESPLKADKPTDRDKLEQIEGIEQEQEEDEDQSPDKLEESNKNLASQQEIAPIKKIKKKGRSDKYISIAPTNKTYKTDIETQNAYQIGQKDRLGEFQGTFHNYDPTQIEDKMVAARLNQYYTEQRVKEVKEQREDIEMVQSMKDWASNKSRIDEMVLQSEYLTAMGSQYSNVGIKISDLYESKKMDLAEEKAYSNYLKRSSLPKARMVQSAVPMAKIVQNTVIPSQVEEEAEDFQSKQADLEKQINSQRLQLLKMSRGACLPGGLINNPNQETIQSNRIMSSSCFKSTQPKRQFSSILKTHSIKQELDSQIGEIKQLKNRLASANKFVPINILQKSILIPQGHANPYKVPLPKPGILLANLPVDSKQNKSKKKKIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K169</accession>
    <name>A0A8S1K169_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K169"/>
    <dbReference type="OMA" id="ITECSCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="199" mass="23474" checksum="5E340D68BDDCDD90" modified="2022-10-12" version="1">MKNNHHRRTKSSGNYFPIQSEFLVQDDDDDTNETRAIIQKIQQQPILHHQIVEKLDDQNYGLFNTMFNFGQMKYKPKQSLDLSTITECSSTPSTKDAQPKHRKCGSQQQQVNKQPIRHHHSQSLQIHRLEQELEQMKVQQAEIQNVNVMLIIEIHKSRQELQISQQKNENILQRIRKLEKMLKDDQKLSCTTNDSLEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K198</accession>
    <name>A0A8S1K198_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K198"/>
    <dbReference type="OMA" id="LCKWIFT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="109"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="405" mass="47805" checksum="F59117D1EAF9A428" modified="2022-10-12" version="1">MGNICNSEQRDEQLSNSLKNTYFSENEDQPYAPIKPIIQNKIDRDNSLQIGVCFDKSEIINQEICIQLWEGLVGNPQRQHQREPGDLQKALYTIHKNEPERFAEVLEQAGGGQIRWFVWKTLLYSCIKYKHQAMKIRQTERLSIVERDVKRTFPSHPFFQLESQSSQLLSQLLNLVGATFKGIGYTQGMNFILAFMLMQSGGNIEQTWSASFLMFYCSKWMLYAIYMTEFPLMRLICSVTKEILKQQDVQLYKHYMKLDIDDSYLLCKWIFTLYLYNIPKQACLHFWDIIFQNGIFIIASINCQLLISIRTSILSFKNSTQFLEYAQELQQNQICQNYKEIKNNALKTIPQKKIILFCINSIKDQYEKNSSAQEYFQLLQDNFEKSDFEKKYQNWMENNLQQNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2W5</accession>
    <name>A0A8S1K2W5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2W5"/>
    <dbReference type="OMA" id="NIICEED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="212" mass="25307" checksum="B3E1D52B4BA8C4E0" modified="2022-10-12" version="1">MQELLIKLFNIICEEDQTKQKFYKCNNTCEQLKLITDIIIKKQSEFLSLEKQLQKYESEVRKNIRDQYLMQQQQDDLQQKVEELEKSRNEFLSNTKNTIIKLKRENEELYQKCKSLTAQLQKIDNQSISKYSTVENTITNITNTLDQTKLRPKTNTTPQKLKTDVDTSQKRLSQQLIKPNYFQKKQSTQISIQNKRQSYQYMENQLNLTTSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K385</accession>
    <name>A0A8S1K385_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0130244</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K385"/>
    <dbReference type="OMA" id="YTEDQDN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000709">
        <property type="entry name" value="CAMK/CAMK1 protein kinase, variant 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="46"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="351"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="388"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="424"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="456"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8049131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="497" mass="58062" checksum="F37BB07393DDFADD" modified="2022-10-12" version="1">MQPYKKVCVQESNIPYSFNEQDEKIGIDYSNFIQQMEGPYHQLYEPIDKQCIGAGGQGCVIKVKCKLNNEVRAMKVIKKINEDKNENFRKEFQNLKLLDHPNILKLYHSFEDDQRFYIISELCEGGTLSQYIEDHYPLKEAEVLKIMKQLISSINFAHKKNIVHRDIKPENILIDDEVTTSIKLIDWGFSGMIQQYEKLSLKCGTIHFVAPEVMEESYDQKCDIWSCGVVLYILLCNDPPFQGTDSNEILFNIKNQGIEFRYQSWKQYSTLVKDLLKRMLEKNPELRPNAQQVLEDPWFESHSSEQIPSQDFKESMIQFNGYTEDQDNNLKQSRFLQAIVLFIATELVHKDDKKVLNRIFRKIDKDNNGTISKDELKSALQQIYSKNQLDDKVDKIFDFLDVNQSGSLDFSEFVAATCKLSDVEEKIRVAFDVLDKNKDGCITLDELFKFIGREDYDRSDCKEIFNQFDQNGDEKISFAEFSQAVQKYVNICKQIQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K5T8</accession>
    <name>A0A8S1K5T8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0130288</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K5T8"/>
    <dbReference type="OMA" id="NDEIICH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03749">
        <property type="entry name" value="proteasome_alpha_type_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000016">
        <property type="entry name" value="Proteasome subunit alpha type-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035144">
        <property type="entry name" value="Proteasome_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8049175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="248" mass="28137" checksum="025CF14A7A7C442E" modified="2022-10-12" version="1">MLKHLYDTDCVTWSPQGKLFQVEYAMEAVKQGSICLGLKSNENVVLCSLKRQPSELAGYQEKQFKIDDHMAIAIAGLTADARVLCKYMRTECLEYKYTYESHHPVGRLVFKVAEKSQHKTQSGAKRPYGVGLLVAGIDPNGVHLFETCPSGNYYEYKCQAIGSRSQAARTYFESWFHLFEKSTLEQLILHGLSAIKKAIMEDEELNEKNVEVGILGKNLKFTHLNAQELKDYIAKLETFSPNSQIQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8P9</accession>
    <name>A0A8S1K8P9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0170409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8051137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8P9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8051137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="102" mass="12554" checksum="CA645E83A50CE1F8" modified="2022-10-12" version="1">MQNNSQNQQRFRKIIESENAFDKLQLNLYCNIIFQYSVKIQNLIQQLTLQLKFNNNTILYHREKIKQECQSKNKLKIYLIIVYYKNMKNIINFQQHKKKKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8S0</accession>
    <name>A0A8S1K8S0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0180263</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8S0"/>
    <dbReference type="OMA" id="CLNYLQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF93">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="366"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="242"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8051649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="450" mass="53433" checksum="A4314CD8B35F58BF" modified="2022-10-12" version="1">MQQHDQLIRNFSNITGCQDHNKCLNYLQMANFELENAIQLFFDLEIQSQQFVAPIQKGNQQEPQPPQRQKRQIQENFELEQSQTTQELIQKYKNYKENKIDEEAGLVSGVWKLAKALFTKNQNYGEEFQKHIQSKKVKIEIKFEMGSFQDNIKIAQSRNFPIFVYVHDISFLKILQAMFQCKSFVQLVNKNFISYAFIANQDTLAQLPSQNIEIPSILIYRINFVDEICLMKQIKLFPQTNFEELTQEIKSIRSAIAKINAQEKQAKRLVENPEQINQSQIDKFRQKQMEMEIKRQQEIEQRQREELLQKQDFEYMMALKQADEKKKKINDEKLKQDQLIQQQQEEEEQRQFMKATALSGLPQEPQENCITIQLRFFDKVITRNFNFTDKIQSIFDFVMCQDDALFLNPRADIDLIQNFPKLSLFDKKEMTIQEVFNESTGEQLIILENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAN0</accession>
    <name>A0A8S1KAN0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cyclin-dependent kinase 2 homolog</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cell division control protein 2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">cdc2-related kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0170191</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">May form a complex composed of at least the catalytic subunit CRK2 and a cyclin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAN0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001126">
        <property type="entry name" value="Cell Division Cycle related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF254">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="10"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8050705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="275" mass="31933" checksum="4AE0870380855AF1" modified="2022-10-12" version="1">MQDNKKIRQFILQEKVGYGAFGDVYKAIDENTNEVFAIKRIFSGKSQQIDQSTIREISALSSLKGQNHIVPLQQVIFEKGCVYIVLPFYPYNLYGFIKKYNEINYKKIFKQLILGVYNIHKLGFMHRDLKPLNILVDENQNVYISDFGIARMNFGQCRNFNSNCEKHSYEVITLHFRPPENLLGCDNYHVSVDVWSLGCVFYQVVMKEILFYGDSQIEMLFKIFSKLGTPSAFIAPSLCSLPLFNHFLDFIQLSIYFSKSYSLKQDTKELIQLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB09</accession>
    <name>A0A8S1KB09_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0180290</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB09"/>
    <dbReference type="OMA" id="VYQNKQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000726">
        <property type="entry name" value="Calcium-dependent protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000634">
        <property type="entry name" value="Calcium-dependent protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000382">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="36"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="351"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="433"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="469"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8051703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="515" mass="58985" checksum="7343265D1D03BC61" modified="2022-10-12" version="1">MGCVSGAELKQNPIIQMRTKNQDLVFTSTEDISKIYQFGKVLGAGSFGKVVTARMIKNLEKQFAIKIIEKTKVKGREDILANEIYMLQRLDHPNIIKFHEVYQNKQNFYICMDYCRGGELVEWIPKKYKSFHEQNIQELMKKIVSAVSYIHDQGIVHRDIKAENIMITSKKDDGEPKLIDFGLANKFDTSHLKRLKSFAGTPMYMAPEVIKGSYDEKCDIWSLGVLLFTLLSGHLPFHGETKEELYDNIQKANISFTSTIWSHISSEAKDLIKKMLSKQPGLRPNSKEVLKHSWFKKQFKNEEKNSTLLNNSLTSSITENRSIYSLLKQNQGGAKFKKEVKTLLINQLSEAELNQLKEIFKKIDVDNSGTITFQELKQALQAEGSPATFEDIEKLINNGGPPISHEEVGKKKKEFVIKYSQFLASCIDERKFITKEKLSSLFKIFDTDNSNFITKQNIKEAFARNGKQISDQKIDEMIAEIDPNHDNKISFEEFCQMFDNAGVNKNFTEEGSIYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB14</accession>
    <name>A0A8S1KB14_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0140334</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB14"/>
    <dbReference type="OMA" id="NRNQYNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="426" mass="50725" checksum="801E65D9FFF0B0BC" modified="2022-10-12" version="1">MSTRNPYNEEIQRKLIEIVDTYGGKKCKSWTQVARIFELQTGIKVQKTFDLKKKWEMFTNYKEDLTKIELKTLYATGVRCRGHHKAGNEEFFKQTGKKLYVNQYRKLVGNFLLSAIKLLSQSYVNPKKVKCKQQGQERFHNRIGYVSIHILLRAVEINKYLDDYMVQELKHSAQKFEQLLLIHAEHHEDERIARFSQYHKVINRREFKKIQFFLDYVNELKQISINLYTEQIGRHPLLFPQIDQNKSSKIYELLIWGNDWQSKYNLFQECAAKSDWEEYSMLIKKTKKNEENKQIKNKQQQSSEIINAETIEPETKKLKLNSYDLISTATHKVCNNASKQQIKQLDKQELKSFRGHYYRDGQIRTKGLTTILFDTDMRSDEDDQDQYQNIEEHRKEFFDKMEEIYDCVFGGKASLVSFEKSLQLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KBN2</accession>
    <name>A0A8S1KBN2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0190253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KBN2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="30316" checksum="3A42081459038131" modified="2022-10-12" version="1">MSLIKAPQSPQIINHFKDQSKNNNNNKISQSVKVEKSRNSIIDEQDEQATVIKYKTSQGDFLQFDNSIQQKGSPNETNILINNNDQSNSLQIRNQTDDAMLSISPKGCCFCGNIQNVFPICQCSQAHQKCADQYLQQGPYLEQILCKDCNQYRHVNGVINFDFSQWKQSKSSLLLECLFLFITLAIFGVLTYFSFFELYDEIAKDRLLISCLVFSFTVWFILLICLISKILSHFKKVKLLIKRFDPKNVQYNQNYVNLMMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KGQ2</accession>
    <name>A0A8S1KGQ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KGQ2"/>
    <dbReference type="OMA" id="HTAQEIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00177">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF56">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="START" evidence="1">
        <location>
            <begin position="25"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="210" mass="24032" checksum="C7AEDCF336A49A29" modified="2022-10-12" version="1">MQANYQAELEQLQTNAIELAYQTIEEGKWEKEIEQDGVVFYSKPGSTGWKITRSELIAEIDPKKAVDVLRDYTRFQEYNHTAQEINLIKKIDDNISIQYILTKPNQVLQQQRDIVTLSRVSSLPDGTFFIVSKSIEVPEAPIKEQYVRAEIILSFFGFKPVENGQTLVTMVQSFDPKGDAPKDLLNSLANKVVNYNKLFVENLKKATIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKT5</accession>
    <name>A0A8S1KKT5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKT5"/>
    <dbReference type="OMA" id="RLFHDPI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="186" mass="21782" checksum="67BE06D73FD8E454" modified="2022-10-12" version="1">MQNQPFKFSTINFSNPNSSFHKYVKDEAFIQFIPESIIEDQHRSSISTEGSYDYSESQKINKKGKISKIKDKDQPLPQKSETKNIPKNFGVLLKKYLCSKNTENQAIKAFLKNGESKKNFSRQDFTRLFNDPIAADLSRQYFSGFQIIHDLMISEKIQDVKNHIKYISKFYNSTYNKQELDELKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLM0</accession>
    <name>A0A8S1KLM0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLM0"/>
    <dbReference type="OMA" id="EMHEKHT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604:SF0">
        <property type="entry name" value="ABASIC SITE PROCESSING PROTEIN HMCES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="DC12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="290" mass="34596" checksum="368248AC4B112EA9" modified="2022-10-12" version="1">MCGRIFQIHNIAQLLQFAQTRRVRNAANFNPTYNMGPTNYIPAIRHVEDDDNFEEYEEQNNQNQEHNQSLDNEQQIEDQSQEIIEKQKNKRGLDFFKWGFQTKFNLIINARIEEMHEKHTFKPFLDKKRCVIIADGYYEWNKKKEPFKFSTNKPLYLAAMYTDDDEIFVLTRDAFGEYAKVHDRMPVLLEDDEIDLWINPKIGFFDIIDKKILNSQKKIWGQVQVTKVAPYVNNIKEKSEKCLITLEEYKEQQFKNGIGKFFQKKQTQNTQETQEQLEKTKEKITKSLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KPZ9</accession>
    <name>A0A8S1KPZ9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0260075</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KPZ9"/>
    <dbReference type="OMA" id="NQISNCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000771">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="17"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8057500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="689" mass="80571" checksum="A5FAF57F82084AF9" modified="2022-10-12" version="1">MIETKQKMQKIVQKYSYFLNDILGEGYSSSVYKGINIQTNQVVAIKVINFSTLTTPISQTLLKNEITILKQLDHENLMKVYEIFETKNNTYIICEYCNDGDLANILEKTTFTQIEIINVFLQIAKGVKALHQQKIIHRDIKPANILRSNGIYKLSDFGFAIVENDFESIIKRFSVGTPVYMAPETVQSNFYSEKSDIWSLGVVLYLMVYKEIPFNLKKDGDLYSKQQQIHKKIMNDKTLLKKIQNILIGMLEIDPQKRISIDGILSMLQYSKQLKCSNSHKNIPFKVLRRSSGYDQILQDGCDQDKFENSQKILKTLPDELDNINEEIETIQFHNSHQYHHILELNNIQQNQLFNNENQQLKFESSPLQIKDKTKKQIQINIPKNQSTNFKKLNPNFNQLPYENSDTPQHSNPNSTNDTIKNNQISNCQPQNQMNSNSPLIAPQITETLTSRNQRVSPIHINQSLRNLIVSPLRKKIGPTTYSMTDFKNFQIKPNELLLKKNDFIQQRAKTDLSINKLSSDQDKKKQDKEKVKLKSMIESLTATIRPTYKFLLFLNQTLKKFQSINTEEKQKCYFLLRKLQAVKAYAIKNQCPSQIQEELQCWIDSFQQYFEKVSPLLYNHHDRKFIQYLNTNLEQFTKGFSQQINSYIQEVNQQLLSKELLIIQEVINENLKQFNDPILFARRWENEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQ13</accession>
    <name>A0A8S1KQ13_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQ13"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="74" mass="8785" checksum="045A166375DC297D" modified="2022-10-12" version="1">MFSLMKNIKRFIRSYDNFGVSLNFNIRNRKQYQSVCGGIMSLAMTIVLLYIFITGLISLLLRDKFYVLQLFYQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRC5</accession>
    <name>A0A8S1KRC5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0200358</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRC5"/>
    <dbReference type="OMA" id="MEMENAF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="144"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="297"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="420"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8053494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="811" mass="92777" checksum="7D2606CE4DA67241" modified="2022-10-12" version="1">MATIKEQLDQKIKAQLFDEAIQLADSIPENNIMRFFYKTNCYLAKKNWNEAINQAQQGLSLNIPPTQKFPLYGMKAQAHMALQQFNEAAEFFSNAYEIQKDKTDQAVLANYGHALMNSQPQQTEKAIEILLLAISKGEGTPNGFTAQGNLSKIYFAQQNYEKALEIYEKLVKMPSQEKNKMLILWKNKAACETNLNKNEEAKKSFLEAQKLDPNDQDIQRGIATILQNEMISCEKKHDYEHCLANAEELLKIVTANTDPLKQEALYCKALSLKGLKRGREQIIQAYQELLNINPNHTHAISNLAVEYYAQENFEMAIEFYQKALQVQQDPGYWSNMGQAYLSLKLPKFDKSLDCFNNALRLNPKAVLSLTNRAMLYIRLSKRQEAMTDLKLVSTLIENPENVADLSEKQLQYIKSGVHKIMEMENAFEREKKAAQEALAKQNQQDPEIIRIQQELEKIQSEIDAQNAAELAQMAQKEQQTTVKKVSKEEMKQRIQQLLAEAEQSQKKNEEKVEEIKIEQKKEDIKIERIEKKIDELEECIKVIGIDKVAKVKENYAKWRTEYVEGYNYAKAFYWTFINYCDAQKIASTEIINQENGYSVGTTTKVAVKVTQAVLDFGANIPIFGAIFSAINGAIGAVYQEYEKFQDEKKRNAILKVIMKNQDADDKEGWSMLVATAAQLIASRKRKYFEAGRVEKQKSRLDSMIDWVQDKIKAIKDAASGQKVDLYESWGGQEATEDITILLAFIMKNHEEMVTNTSQSLDVTIADIIYLEDGQVSASFQVMLDMVKKREEIENPKKETERTDSSCSCQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KU60</accession>
    <name>A0A8S1KU60_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MIR domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0280013</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CENVHIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF4">
        <property type="entry name" value="RYR_IP3R HOMOLOGY ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2300"/>
            <end position="2316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2341"/>
            <end position="2362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2481"/>
            <end position="2503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2557"/>
            <end position="2580"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol 1,4,5-trisphosphate/ryanodine receptor" evidence="7">
        <location>
            <begin position="20"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="2442"/>
            <end position="2590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8058778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2682" mass="318457" checksum="FC5450BD09A5CD78" modified="2022-10-12" version="1">MDQDESWGSLQQIIHEDENQNNVRQGDIIYLKSYLPDDNKGLISGEGFATNKLECLLLQKQSNEYLNSKEILTNKQTDSLVNNQAEWNIFKCLFRILDSNRSIYEQSKNEIVSDQLKEIEEQNECEKNIIYGQEIQLLHLYSKCFLTLNSQVLAKENCCRELRLEQSITSNSNFMLQSNTKSYGEPVLYGDILYLKSSVIHLNKWGLGIQQTSPYSEVHASQIASTLRICNYLENENENIIMNGDIIVFKNRLFGGYLSIKRQFSSTKLMEKRQIYKKDQVLEQNYINLTELKQQENLDKMFHITLESELQLNSLWQLIGIASQTIQQQNNKKDRNRVQKSYLIKNVLTGTFLYLENEQLHASFDRLQPKFEFQIRNLQKTESQINLGEFYRILRIQENPQLSQFQTYVIQCNNSNEVNITTNCQKTQFDQQLFKVARGPYQDAMAANKIQSFYFGIVEFYIFLQDWALDKELKYQYNLAFQNQKQLQDEINQFEKLLYNMNLFLSQENSIQEIKQCQFNLCQFDVLDLLIKLQQLVNLISNQENKWASSYSDKTAQRIARQKFDPVASIKKKKQFNLVEEIYQTIYQIIFDNVDCCNYVIQEDSIIDFLFSQLNQFREQIEKMFKQIVSKAKYNPIIAKKWINRIQMIKEENLEDQMFIITIINLFISSSYHISIECQKLCRKNLFYNNKCKLLKCLIIEELPIIQIEADAYNFQQNNIQFCDNHHKYYQNLSQNQIAIEFFIKHDQERLYRKYEQYLLNLLNLYAKMCRGRNFKNMNLIKNVAGINYQFFDSIIKTSHSLQCKQVIIELFSSLYFDIDPLTKISEFQNTCYLRQALGDFDKELESGRYFYENISSNILKQTDSYQNFQQDTLKTQENMKDLLLLKSFSSHIITNQKYHEYFISLFLQQEYPTILTEDSNSSLKYKEISKIQLKFFLSVMRIIKDSIDLGYNTLNKNREIQETLPNIFFILIIQLNNKFKTFEFQEDKAFILNLKNMQQAQCKQISVQFERFNLRFNQSWIADFLLWSTSTCNDQDLIEQIFQEALNIYYVLFYLQANLQVLQYLTQKESSMNELFANHDSILFHCLFGATKLPNLNNEILQYLILSFNNQQYDLQEIKEVEILDDEIERKYFGIVNGHYEAQDDQGPKEIQQYVKQAVKYVQIDQQNNKKLDEFEKSFKKISQYIQKVNSLFESIKIYNQSYTKSLQNMIRNAGIHLIFIEFFINSDQIIENEQVVYFYRNFLKFWENFLKDNQTNFQILIPYLDSLLKIILLPQPQHDNFLYKSLQISKLQLDSASHIIIDIIMNICKQLNLNENITHIRAAKQYLKIIKEFTKTASKPKFNHLSNQKQILHLILQNFRNLIDPLQYFEDKNLKDKQIQSSILRYYQLKLHSAALNLISETCQNYYLGISETQRILLFEQLTSVLQNEPYIVKAAYLKCIFELYINFVKSNNLIGDLIDNYQLKELITSIIIPDLQKMQQFQKSHNLENLILLNNKEYWKYILQVFEFLISVIDEFRVPERQNDINFVEEYTKINTLIKGILLNSSSPLIQEKREYIQQISEIIELFVKNQVEFNLQTRLQIYCIRQKISVQFLIDYIEQMNQKDRIQKLKEICNQNNKIYIKQLDLSLIRPNKASHQQSSENWVDFKKAFNTQIDLNEITTQAQERLKEELNLQVLKEKFQERKEEEIKKTFIKKGYQGEILNPLSEDVQILFFLHELSQQADSLLQEEKFKEFIIKCKQIFSQYPNYLIKFCKIFLEMKKPIKNYINQGEIDEEEDINERGNQKDNQNTIEQFTTFQNIIAECDIHVIAIEQLNHQNTIEQLDCLALLHLLLDFGNSQVQQRFYTLLQNHKKHKKIFIQFFKDFYSTDVGIKLEELSLSKNTNYLNICIKVLQVLQGLCENVHIEFQKFLRIQNQQDSITNNFNFVLETTNLMVNFLLDKSVDNSKKWELYQQGLLSLIEFSTGLNENKKEITKNSRLFSGFNELFNHADLDKALVPQSKKISNIYFQLLQTLYIYIQLLISLLEGNANENKQVYKFLLENLNNEFLMSLAKKIYHIRINPKKSVICVDKYCRLPCKDNLCNKSFCINGYKTREDSLLIETGLNIFIIGLKLSNYSNDQKFKIFQFESMTIEKDLIQEEQIYQNISLRNVIEKTFSISQHPEYYQRVVDTQQVNNELFDDISEDKQHTEAKLVQVSREDEFFLFYREFIGRIEVVNQFGQIERILFQKPFVCKYMTQNIKQNLIYESNRETDEDRITSFLENINFYHSQMAHYQNVSTIPIMHFGQKHWRTLKDISYILCVVIVLFFILLPDDIADGNLVQGDNDEDEIITDSKKVQIIITVIQLVLNLIIIIFCAIERYPIAVNFRRGETNVKQIQNLKIEAGFKIPEYKKKYYEFVGYMENNFEKQQVRKNPFRKIIVLLLIDFDNFYNLIIFGITAVAFFNNYVYAILLLDIVKRSEVLQNIIKAFTENTKNLLIFGLLGLIGLVLYGFLIKLSFSEDFKDDTKFASDSLGYSIAHVINFGLRNGGGIGDTLSDYADPFEDPAKYWKRYFFDITFFIIFNILFLQMIFGIIVDKFGELRDERQELLKDIQGKCFICSLDRNEIDSKTKNGWYQHIYLEHNVYHMLFYLIYIKKKPITECNQLEKYIKDQMENRSSEFLPVKMTYELQNQQKQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVK1</accession>
    <name>A0A8S1KVK1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0290031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVK1"/>
    <dbReference type="OMA" id="WDVNPFV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8059520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="206" mass="23816" checksum="1796EB8E366058A7" modified="2022-10-12" version="1">MLQKATIRYYLSQARKIPTIWDVNPLAYQRMSNALLGAYSNRQFGVLIRENLDMITDHQVAYAFQRINELNLDADEDFYEYVLPALKEFVPNLTKDHPVSIATIIGTCGKINVKDASLWQLFEKKIVADRLYRYIPLNDLVSMTQQVALSDYGSELFFNTMEKQIGRHRLALSDEQIKLTQEAFERKRLAAPIIKQLKQSAQKQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWA0</accession>
    <name>A0A8S1KWA0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA polymerase kappa</fullName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0270077</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWA0"/>
    <dbReference type="OMA" id="KYRTYDT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03586">
        <property type="entry name" value="PolY_Pol_IV_kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.100:FF:000004">
        <property type="entry name" value="DNA polymerase IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000134">
        <property type="entry name" value="ImpB/MucB/SamB family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000012">
        <property type="entry name" value="Putative DNA-directed polymerase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050116">
        <property type="entry name" value="DNA_polymerase-Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022880">
        <property type="entry name" value="DNApol_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024728">
        <property type="entry name" value="PolY_HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002677">
        <property type="entry name" value="PRK02406.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11076:SF33">
        <property type="entry name" value="DNA POLYMERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11076">
        <property type="entry name" value="DNA REPAIR POLYMERASE UMUC / TRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11798">
        <property type="entry name" value="IMS_HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UmuC" evidence="14">
        <location>
            <begin position="88"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="557"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="500"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8058165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="593" mass="68021" checksum="26F4A70AE09A5C4E" modified="2022-10-12" version="1">MNEPIFHFYGNKAGVEGADQIKQVIDDATRGSEYYKRQQEKRLQVLQKVNKMQIELENYNKNEQRKKDIKEIIQNKVKEFQINTDRIWAHFDMDMFYVACELLDKPELIDKPVAVGQSIVSTANYVARKYGVRSAMPTFVAKKLCPDIIFIPCHFEKYKSVSNIFMTILKKYDDNLESMGLDEANLDLTQYINNSQEDPAILCENIRKDIFLATQLTASCGVGPNKMIAKLASEINKPNGLHVVQQSPIAVLSFLEKLPVRKIPGIGNITEQILSGLNFNTCKDVRDRADELYIIFTPKTFEYIFYSCWGVSRNYHVEFEDQHSISCQRTFSSISTQKEFEDKVDYIAEKLAEEMQSEEKVGNHLTLIIKTSKFEIRNKSLQLNQYTNQSKQIALYGKQLLKIMQLDEPIRLLGLKMSSLANEKQIQKQSISTYFKKQFDKSNDQENQIASNMGSKSHNIYEDSNPILKVQLQQPEQSKPEIISDDEVSSISNQIISGVNSKSNQSQAQQQKFQQKQQAQPKSFNCPICNKDIDCKGNNTVLNKHIDRCINQQNVIAEQSDESNKNVKDSKKKVSSKIENKAQKSLNFFKKGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KX65</accession>
    <name>A0A8S1KX65_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0280360</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KX65"/>
    <dbReference type="OMA" id="ECEGWRT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035823424" description="Trichocyst matrix protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="262"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="262"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8059357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="40588" checksum="6A3AB22941413167" modified="2022-10-12" version="1">MNKVLLILVVLSITQSVFVKRSNDPTKAVFTQLEAMEEHELGKKLLDTIALQLNNKAPLSDIAKMLQQLRENLILNQQEADQKHAQDEVDCETEIYQYNRRIDYASAEITDSTTEIATLTSKVEQLAQDVDNKKIQLDILNEQETQLTEQRANDADDFIKFEHETENVIEAIEVIFTKLSSIQPDQEVVQILTQLNQIGASNPILALMQVASTFSKDQLNNVLNKLSEVSASLEQSLHDARQSEIQAQLDYERLMVEIESQRESLSSAREDSERQLKDNEQALDLQKKRKEDATDELNAATSGKEQKEGECDGWRTQYAADTEHRQQEISIIRQIEEILATKLKNVKVYLLERSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXM5</accession>
    <name>A0A8S1KXM5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DIX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0300020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXM5"/>
    <dbReference type="OMA" id="TRISWET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001158">
        <property type="entry name" value="DIX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42509">
        <property type="entry name" value="DIX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42509:SF1">
        <property type="entry name" value="DIX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00778">
        <property type="entry name" value="DIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50841">
        <property type="entry name" value="DIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DIX" evidence="2">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8060209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="150" mass="17641" checksum="9FC4F2371C8DEB51" modified="2022-10-12" version="1">MKQFTLIYYVVPQDNDDIEIPNAFGIGKPVDQITLKDIKTSFPLQGEYIFRFRYKTMHNTVWLDLPTDTSQIPLFNNRILIKATRISWDVQNNQNQSTSVSQQPNVLPQQQTQNQIVNQQTFAQFQQPQQNQQQPQQQQQQSQNQNLIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L048</accession>
    <name>A0A8S1L048_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L048"/>
    <dbReference type="OMA" id="IMRYESQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="229"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="273" mass="32505" checksum="D56F6E052FCA46FD" modified="2022-10-12" version="1">MLKFLENRDPQPTKRGKDIMRYESQSHLPNNRQFNSPLHQDALIQNDKPTYYITKKLPPIQIYNPLIQTIDSVERPEYIVKQKDIYIPRYLNEYKTKGFKKKQGIFESYQHLLAQNKSLIQGIHTDKKPLSLPAIKEHQKEQSILNVPAQFSTRSQDVQKIDINYFKSNLSIDQPQIKVKQNGNKNISQKKQRISHKLITEDKGIQVDIEKVKSHKTTEISADYIYNFNFQYQQKLEDEEEKEKQMRQLSIFDKKIKEFKEQQKQKLFKKHVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2U0</accession>
    <name>A0A8S1L2U0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0300169</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2U0"/>
    <dbReference type="OMA" id="HITVFQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="119"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="386"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="429"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8060505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="461" mass="53698" checksum="CB0574376DFCC3D2" modified="2022-10-12" version="1">MNQEKKSFFDYTSPFELKRPIEIFSKSLKDQFYILKNDKFKLKTLVLQRNYLIKFSSKKSNQKYYNITNALLELVNHAQLGTGISITKNGANFTFYGIVDHWYSYMKKWCIQPNFQKEYCMIKLIGKGSFAKVYKIQSLSDKKEFAVKLFDKTTFKTQDRPALLKEIELMRIMNHKNVVSILETYENEQYIFIVQELFNGGELHQELKKTRTFSEYEAFIIIQQVIEALQYIHSHGIIHRDIKPENIILREQGMIEQVVLADFGLADYFRKDCKYMFTRCGTPGFVAPELLQDKIYDYKVDIYSCGILFYYLLVGKGPFDSNNYDQTVMANFNGQVDLTKLQFSIECLELLRGMLDPNPIKRYSIDQIKQSQFFRKHTTVFQQYGSQSSLGSIPSQGDSRTTSPLQSPKQEAKSPNDYKSRFVNPYASPLQSPTRQNAPSFSPLQGPFGKNQNLQRIPIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2V6</accession>
    <name>A0A8S1L2V6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase C51 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0300184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2V6"/>
    <dbReference type="OMA" id="GWINIPA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007921">
        <property type="entry name" value="CHAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051705">
        <property type="entry name" value="Gsp_Synthetase/Amidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30094">
        <property type="entry name" value="BIFUNCTIONAL GLUTATHIONYLSPERMIDINE SYNTHETASE/AMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30094:SF0">
        <property type="entry name" value="BIFUNCTIONAL GLUTATHIONYLSPERMIDINE SYNTHETASE_AMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05257">
        <property type="entry name" value="CHAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50911">
        <property type="entry name" value="CHAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C51" evidence="1">
        <location>
            <begin position="32"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="205" mass="24135" checksum="372C45C7F47A4BC8" modified="2022-10-12" version="1">MNFPPWGTVLGQPFGVAAYSNIGFVFDRNKIHEYQSYWPKEETGLARDVYIGFKYQCVEYARRFLTLYFKAAFQDVPHAHHIWALDTIEDCTKEEGTFPFVNYPNGSKEPPKIGDIIIYPYSKEQRFGHVGVIINVNLEEKIVDIAEQNYEEAGWECPEYARRLIMKIENGQYFITNQRIGKPYDNWDIKEEIFGWKRIDMNPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L351</accession>
    <name>A0A8S1L351_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0290249</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L351"/>
    <dbReference type="OMA" id="HNSRMIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8059923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="258" mass="31313" checksum="500EEC53F02F572B" modified="2022-10-12" version="1">MHNEFQSHRGKKQFKIESYLQGNIIKAKKLNNSFLFHNSRMISLQNNGMFVYYSKCFKWPTKQFDKHKEKPKSGIYFNNVYYSCVKNSEHEVELIIKFKKSQLYHFENIYESYVKEPRCSDIVLWIFIIPINNIVDNQRFQPVREIDNLHSLFTIKVVNYDEKTQKSISQQVRTEKTTIEQRINKKDFQNKKQVQWLDLQPEKRIHQQNQLPNFTIKYEQLSYDDSWIINEENIDLNIKNMQIKRSSLPCFISFRKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3U0</accession>
    <name>A0A8S1L3U0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0300047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L3U0"/>
    <dbReference type="OMA" id="NCRTFQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF2">
        <property type="entry name" value="GH16429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8060263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="35381" checksum="417333C21AACBF6D" modified="2022-10-12" version="1">MNNDICMFGLTKSGKTSMIRVIFQKLEIFRTFQLDPTNRMESVTVNLGSHIHFKIYEFSGHYDLNDSQPPEIAAMETCSLMIYVIDSQAEPFNEAVQYLRQAIQTVKQRSPHCECHCFIHKVDPDIEDNKKNELLQQIQKDISDELSKNSMNYIKVDFHVTSIFDHSYLEHFSKVIQRILPYSQSIQTLLDSFNLSCKIEKSFLFEIYSKLYLASDSQHFHSTNFQLCSEMIDVFIDVTCIYGKLDDAGQSQIKLSDGSILIYQTVNEGISLISIMKSENLERPFLLEYNINQFRQGLAQIFKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5Z4</accession>
    <name>A0A8S1L5Z4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0340346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5Z4"/>
    <dbReference type="OMA" id="NQTHNMN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="94"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="209" mass="25156" checksum="CA47CC5956AE39AD" modified="2022-10-12" version="1">MVIDNNTLKILLQLEARFHEKLYNLDHVNQMLEIYQDFVQIFDNQKCAMREYFLEKIQFLISRPETIQLLKKQNVLTIPEQSSKVQGKMRFSVYKKAQANEMKIQQELQNNELNQTHNMNQLLQEYDKVQQNIEKQITESLNFQDELLEKRKMNRKMKTQVIIKSISTGNIAQQRQQDENNDEQYIYKLHNESVQRIDKFDFVLDHGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L916</accession>
    <name>A0A8S1L916_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0340058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8062921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L916"/>
    <dbReference type="OMA" id="QNANIRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8062921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="221" mass="26010" checksum="91508BF1A8DA6BB5" modified="2022-10-12" version="1">MKNQQGYLSEQDINQNHKKCSNCYKMVNEKRNSLLFSLAQPNRNKNTSFVPEFQEMPILQRRKSYQEGISGQKMNRLNCIYPQSICMQHAIQKSKIDNKATLRKAVKRASMMMSTVKYWKDHSQKKIEIPTIIKSIHTKDSCLYDKNALTTRSIKKNELPPVLLSQFRIRNNEKLIKYSRDRTADKTKSVSHHHFSNLQTQTIKNFSLRYSIQNANIRIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LA78</accession>
    <name>A0A8S1LA78_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LA78"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="85" mass="10517" checksum="C247CBFBD7FB235A" modified="2022-10-12" version="1">MLLDSKSDELYTDKDFQLDQAERYIEVFNQLKNQNFYELDLDKMLELTIYIVNNTHYQQKFKLSEDRKQVTPRELLNYIEKLYRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LAH5</accession>
    <name>A0A8S1LAH5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0350296</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8064099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LAH5"/>
    <dbReference type="OMA" id="WVKANQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8064099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="177" mass="21403" checksum="63A44C5A735077AF" modified="2022-10-12" version="1">MKFSVPLSHSPSNFSKQLIQQQRQLCLLKVRKQLQNLNDPFDSTEISRALRNLETQQQRKQLLDSIIKMRETKRTQEEQQKQEQLQRLWVKANQTNSKKLNTNYVYHRDLEDFESIKQSRYRFESERIENFYLRNEGKHHNFHRVSSLACLTTDLEKKKKLIPLITRKDRKNFTIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFK4</accession>
    <name>A0A8S1LFK4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0380010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFK4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="66" mass="7655" checksum="A6AFD6E2DC747EC5" modified="2022-10-12" version="1">MSQISNIVHQMNINDSILTKAGLYLGGQDQKRDSKKKKELQSSMNQIRQEAKNQLYYQNSENLILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFN9</accession>
    <name>A0A8S1LFN9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0360333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFN9"/>
    <dbReference type="OMA" id="IQVWPQV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="31406" checksum="1AB4BB173E9D0B44" modified="2022-10-12" version="1">MNQQISQEYFIHKFASISLSETDDDNSKQSSPALDSRLLEFVGTLPAHIKQKLENFKPNSDFQISKQNDDSVYFGTLELGLKVGIGIQVWPQAGNLLIGTWQNDMLQGVCTMHYRNGDIFEAQFQNGRTNGFGMFKSEKKIVKGIWINNQLEGEAQEIKSDGTRFYGQFHNGKKNGRGIQVFPDGCKYEGSFENNQFSGYGIFYWSDGSYYQGQFQKGLIMGFGNYINNNGLQLIGQFSEIKKTQAKEVGLNKNLQSIMYLNQYNQSLLIEKIHFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHM9</accession>
    <name>A0A8S1LHM9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHM9"/>
    <dbReference type="OMA" id="INQNTHV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08060">
        <property type="entry name" value="MPN_UPF0172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005366">
        <property type="entry name" value="EMC8/9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12941">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12941:SF10">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 8_9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03665">
        <property type="entry name" value="UPF0172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="21175" checksum="E9A5DAE52DCA2622" modified="2022-10-12" version="1">MDQQLEQVSVDIQKIVYKKAMLHACKYSTEDVIGILIGQINDKHVNILDAYPLFHSRVSLNTLEISLDIISSELQSDRKIIGVYEARANTRGIMSEIVQEMLNTINQKQAILMRISQIEVDDNLVLNAKIVSLNENVKYVINSSSTLEWKIIQDCLKAGLHWKIIDFDAHFENVQLNFRNQYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LI56</accession>
    <name>A0A8S1LI56_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0410206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LI56"/>
    <dbReference type="OMA" id="NLAYFQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="455" mass="55508" checksum="2FB03161310D8134" modified="2022-10-12" version="1">MVQELQQILNYQEILKPINEYEQIKQQSGQVYNYIYEQIQTDQEFLDLELKIHFNKLQNQNENQESTLFNLIKIKQCFIQYVNILQKRNQVNFKFRLIDKYQQNFFQFLQHQLSQNQNHIKVQLDKGHINFSFNIYVNQNTKIEQLKQVMTNNLEMILNFINKYEKQKMYRQSSKINSIKLNIDQSKPFQKSTQLIQKYYVKLFEYLKVVNCEPESQTLITQIIGYNNLFKIQYKNSFDIDNSSLLLIQNGELQNLEKKINSLLKSFDINIQEIISNLFSKIYLECQLKNYYQFIEFQKLSEYFNNQLQQLETFEIRIQLLIQILCIYLLKRQLNLDLAFPQKDVIIIDSKKQIKLDMFTYLKYKSIKNDKLEQSEELYQIQEIQEIFNYFQLLFPFRVIIFILLKEKQVIIENYNITKEIENMKQEIKENKINLYFFIFQLIDIEKLIKQYLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LI80</accession>
    <name>A0A8S1LI80_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0370124</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LI80"/>
    <dbReference type="OMA" id="AINECEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035762924" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="34"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8065153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="247" mass="28891" checksum="AF6F82CC0E02D483" modified="2022-10-12" version="1">MKLFLIILFTIGLAINECEVDNKDRVKLSGMSKQKEDELLELHNEHRNLVAIGKVRNYYGEFSTATNMNCIKWDDHLAKNAQDCADKCPSNFKLDCSFPRHYGYLTYKGEVDNGGQDWSATRIFKKLISQEDFARQIELATVQFFGCGRTQISRRNGKSQEIFVCVYNKQQNLNGEVYKYGIPGQDCLYGRKIEFQGLCKQSITDIESLKFRHQRHQDAYKYHHKDEKHHVHHNHHRKDDGFHTRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIG7</accession>
    <name>A0A8S1LIG7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">acylphosphatase</fullName>
            <ecNumber evidence="2 5">3.6.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0380105</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>an acyl phosphate + H2O = a carboxylate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the acylphosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7" evidence="2 5"/>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LIG7"/>
    <dbReference type="OMA" id="KMQGPRA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000011">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10029">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10029:SF3">
        <property type="entry name" value="ACYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="7">
        <location>
            <begin position="7"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="22"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8065812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="102" mass="11819" checksum="5E097C3FD8062617" modified="2022-10-12" version="1">MIKKYFSFDFQVIGKVQGVFFRKFTKEQGTSLGLVGWVENQKDGSVKGVAQGDPKKCEQMIHWLSNVGSPKSKIQKMIKTNEREIDKLQYKDFSVRDDKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJL7</accession>
    <name>A0A8S1LJL7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0390025</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YERNRIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000061">
        <property type="entry name" value="Sodium leak channel non-selective protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000030">
        <property type="entry name" value="sodium leak channel non-selective protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000525">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000082">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000113">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000117">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="364"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="480"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="510"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="544"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="589"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="616"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="698"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="929"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="961"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="999"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1054"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1166"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1246"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1279"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1310"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1369"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1472"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="16">
        <location>
            <begin position="113"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="16">
        <location>
            <begin position="479"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="16">
        <location>
            <begin position="930"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="16">
        <location>
            <begin position="1245"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="862"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="402"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="869"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8066312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1698" mass="198855" checksum="79CBD4C5BF33B3C3" modified="2022-10-12" version="1">MSTTLKALGIEVLNFTKSRQEEAVHTQSSLRITPFLKIDSETFDWSTIIAKPELISIKGKKHEQYINAETMEEDLLQGEVQWPYAENSIYLFSSTSPFRLSLLQTLSSQKLVIFMRLITIINVAAVCAYDYQSRIEQEDYERNRICTTIEIVCNILFGLECLGQIIVQGFLLGKNTYLKSGWNIVNFATFISTWAILGNINSSNQLIHILRVIRIFRALRLIQDSKILKKQLDAFIGSFQRLGPILVPLLFVVLYYSIIGLHLFMGITEYRCRETPEPENGKWIAVEDIKHLCGIWDCPEDTYCGSLADHGLPRDFQENRYEQFGFGFIRFDDFFYSLFVVFTFLTIIGWSGTTFMFWRAMTTYVTAFYFVSLIFILAYLLSNLLLASFYESFMVLSSIKQNKNSDNEQEALENEIMKKKQQQMMTRLQQLNDQKNQRRIKKKKYEISLYYEEEEQENDILGEKFKQFQHFSNIIVKSNLFNYLSCSMILISTIVIISDHHQISDEIYQNLLIVDFICIIYFILELIITIWGIGMEQFKEIIDIFDSLVILSQFILIIVLFFMDENIIINYNNYINFVKALKMLRLMKFLYIARIFYSICVLARCLVQTLLKIKDLIFLFIFLIIITSLFGQELLAYKVRFEELSNGELEISHSQEGISAPINYEGFGNALMAATNVFYNEEWHITMFIHAQKIWVSILYHIITILMGQVLFVRLFLAVFLNEFCQQLKKIEQEIKPINFILQSKKALNFIIQLIKKRRRSKIHSQAQLQQQVNPQSRQSAKSPSRISMFHNLNGIKLQKLPESTNNELIDQKVQTPSNIFSNKESMIDPLKKEDKIIQKNIDNKNIYEDDHIQDHNIDEHQNEEDMDHDSSQDENLKSPDDPSHPVQSKNLSKSIRKSMRKSNSEKTLFIFAPESDFRQLVTTLVTNIYFRIFNFTLILLTCIRIALLSPLEDPKSDLSYVLQIGYIFLTVFYILVILLNCIAFGLYKTEQSFFRQSVYNIFSFAITIVDLITLIFDITHPLSKFITSLRILQFIQIGAVFSKNIAYAQASLLNAFTQMIQLAIFCIIILSIYGVFALKILKGTMYYCDIDDNKFKEFIQTSTDCFDYGGSWINQILTFDTIFSSILTLFCVATSENWIPLQIQAWNSVGINRQPIQSNNRLFSVYFQIFFFLGFLCLLNMFIGLIVNAYQEAKMKAQNLHLLDETQREWFQIKMQIYTMKPLVKSKKPENSLRKLLFFMVKQKYFKIFWLIIIFSNTIILAIHFQRSNENYKYALDIVNDIFVSIFAFEILCRFFAKEKHLYFKDISNIVDIIAIWWAIANLFIKNKDDYNFYFKRISNAISVAYQLQRNYRIVKRFNNLEKLFSSIFSVIPNALSMLFIMFIFLFIYTTLGIDMFAFIRTQTSLNGWDQHFRKFSTAMFALIKVASSESWWTIMIDTLHEQSPNYACNYMSTYEEYLIYGFNGCGTPYAYLYFISFHIIFSLMILNLLIASVLVAYEEHVKSEESAVSKYQLNDVLSLWRNYDPEGKGFINYKDFWKLSSEIAIIFGVAQDDLLDVNNKKNFLKVLNIPIYESKESNMLCYKFHDVILSLTKISVTLKYGVTNLEPTDKIIQQKLLVQLGDLQRKDFMNQFTPTSFNSGDMVAIIYIQKKFRLWKKRVQVLREGGDVKAIYNEMSDLKNMIKKQLELEEQQEKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKR1</accession>
    <name>A0A8S1LKR1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LKR1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="71" mass="7975" checksum="8A4E3652AC4DB78F" modified="2022-10-12" version="1">MCIEFAFKRGGITLIRNFLHSAEGVKNGLPSVVQNRLSINYKLRTYTQGKVTDIRFITDPVAGYQAKGDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LLD2</accession>
    <name>A0A8S1LLD2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0350038</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LLD2"/>
    <dbReference type="OMA" id="RSACIMC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="43419" checksum="DAA6D43B5F986AC2" modified="2022-10-12" version="1">MKGIESLNKSAYFKEKYFNPNNIFNQIETNLRVIKEKCEQQILKQNSQYQIEMFQEVLDHLNNIIQCNSDRQIIFKQNHQLIIDNQSQYYQLTNYNMDSQMKYGVTLIFPNQITIEEIFSDQLTFKEFYHFLLKKFSDEIKQMFVILDQDTNIINPDDWDQPFIYGNKQHSLFFIQSLQFYIMQQQQQEIRQETYPINQKVQIQQNDFKHQLFQSHNIYPNKDIKKESLNSQFHSSILKNGQSLMNVIQYKCQQCNQIIQNKQIMLKCFHNYHEDCLSSMLKIQIESNQPTLNCLCNKKIKMNEVNVLNKVQIERLYQNQINLIYQKQQALFIKCNKCQFFYIKAQNKSQIRQVCLMCQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LRF9</accession>
    <name>A0A8S1LRF9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0420221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LRF9"/>
    <dbReference type="OMA" id="QAYTACI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019140">
        <property type="entry name" value="MCM_complex-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13489">
        <property type="entry name" value="MINI-CHROMOSOME MAINTENANCE COMPLEX-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13489:SF0">
        <property type="entry name" value="MINI-CHROMOSOME MAINTENANCE COMPLEX-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09739">
        <property type="entry name" value="MCM_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8068561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="446" mass="51685" checksum="52CC730C060C42CF" modified="2022-10-12" version="1">MDQILLDVQQMTQERVLEIKDQNEIRNYFASIQLSNQSISTLESLQPQNLSDQLVYLTIQIIDVLDQDVFIGAVSQNNQVIIYPLGCDSPINGQPLYFDKQAYTACILDPNHLFKPTKSIVTLYSNFNDSIKPKIFNKFNILALKHEYEDEIFFNVIKYIQPTFNNQINKQQLYQQLINNLNLILKDHLVSELLALHLLSSAKYETYAEGARQILNIYGLTKNDADNLKSVIQSIYSPTLYIPLEYEFLSNIETLSKKNFDENTISQGLLCIPTNINSQIVVDETLMKEGQISGKTLQNLQNIQKFIEHSKIGIDFQYSMVEVPVQTNVLILSTGKSFIQTSDSIKVNPQNHIQNIPVNYQGLEEFVSQVHQQQQMQMNIDEELKKKINSRYIELRQQNNLKIDVNNLNNWILQMKILSLIKQEESNYNNFEYAVNLDIERIKRNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LW38</accession>
    <name>A0A8S1LW38_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0440255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LW38"/>
    <dbReference type="OMA" id="KERNHKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="189" mass="22612" checksum="DAE947BE2A8CC3B5" modified="2022-10-12" version="1">MSESGSNSVSDDQEDNQQKNIQDEIVDGEELNQNQVNAAKQANLDRDIRKQHFFTINPEEDLKMVKLKHYPKSSQLDAIYNQYKRIQQLKSSEDEVFLKLEDLQFQKERKFTISEVRALRKERNHKYFKYLIEVRKNFVNEELFSKFRKKVKVFSFDENAANKEKQNEKSIDESVEKESDVFSNYSEFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXU0</accession>
    <name>A0A8S1LXU0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0450175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXU0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="150" mass="17493" checksum="42CE07360988D376" modified="2022-10-12" version="1">MDVKTMLTQIPSFQFIINIYKSIVDLFTRSSMKLQSQLKNLDIYILYILISLLNSEIMHQETYDFNNVFKISISYCNAVKLFKVSVSRYIRKQKILYIQCSSNIQNILHLKLTKSKFALLIPIITKKQNHTLNSQFKQEPKQPGLGIGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LY20</accession>
    <name>A0A8S1LY20_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Polyadenylate-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0450232</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LY20"/>
    <dbReference type="OMA" id="QQPGFMP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001548">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:002151">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001005">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="71"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="154"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="245"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="5">
        <location>
            <begin position="552"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="450"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8070431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="75337" checksum="8DD57AF76907CF32" modified="2022-10-12" version="1">MTILIYENIVLNLYRDFFLKFKSLSFGINKISAKAGIAYRKNQRNFKSYLLNQIQMFRFPGYQDQNKQQENGLHIADLEEGITEEQLYVEFRKFGQISFVKLHRYPLIGKSKHYAFIYFSSQEEARKAKEAMNYKQLLREPMRITYIQEYEKDANLFFAGFELTITLKQLEEFFQKWGQVVSVKLSIDENKKSRGYGWVQYEKKEQANALLADSTDGTIMYNEKKPIIVKRFVKKGQSDREDKRNNLYIKNFWPSLDNYDLDNAEVRESLEKEMRTKLDEWFNGFGQIVSILVKIDIERKAPFAFVSFNRHQDAKEAQRTLGTNQKRDPLSTGRMMYVGWAQTKTDRKQQRDNNIFAKYIYADHLSRNVTEEMIRTTLNECGYGDIQMIRLEKMQQGFQQIIRIGYIVFEQASDANKLIKNFKSNEKFEEIKKLFDPNVETAGGKYFQHLFPQQSQQRGQRRNNQRQSPNRRMNAMPQGPMPFGQRMPFPPYQMQQRMQGGPGGMRRPFQQQIPGFPQPIRPNIPPPVQQPDFQQQAPAQIVPFNKRQDLVDLMANLEAFKAKPQEDQLRQIQQMLYYRIKARLNTDTEAHWKRISEILSDPTNYSIDEIIDMFKEEEQFNSFVDEAIEQLKEASSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYM5</accession>
    <name>A0A8S1LYM5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0460060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYM5"/>
    <dbReference type="OMA" id="KYYQPNK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="365" mass="43357" checksum="9905FC17566F98A4" modified="2022-10-12" version="1">MYKLNILASKQFFDLQTPVNFLVKKKNFANVREIRQKNNIELGLKMGSYSLPHNIWINTPLTTNRIIKPQGSLALHQKFNKLPIYMGRKDMIKELEKFKQRASSTSPSKKQKKYHNLFYNNLERNEKLKKYFKASSQNSPQKLNSLSNFDESRQQIFSSKKFYEINYQRLNSVKPSIQPKFPQLKKTQMTESSEIDNSELMNTSINQNIQECFSPKKEKIQNQKYYQPNKCILQRNKFLNDSYRLYGFVTEIKEQKQKYSALQNSNIINLNKSGDQNNYANSYKSRDVTPKDKIMKKSKSSKDKLQNLLKQSHQKNHERHFKTPNKKHKSPKKEEYEIQKNRNIVLSYFPKQDLEQILKKKQSQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3E3</accession>
    <name>A0A8S1M3E3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0530210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3E3"/>
    <dbReference type="OMA" id="DMSKHAK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="2">
        <location>
            <begin position="100"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="444" mass="52478" checksum="555F5233E21306C1" modified="2022-10-12" version="1">MQNNKSATEYLDSIGQQLDFIDQYYIQQADQNDAQVIQKFKKVYKYIDEAYKSNFINDDGLFSVYGFFLQTVKKFKRKLENRIYYDKTDYEATLEKFERTQTKLVPVYKDLILEAQKQERIDLETLLDSNMIHHIYTKKELGQFALQLFDFLNLDQIKVPKEILGDLINEIVMNYNVVPYHNFTHAFQLSQLLFSCYMKSDLKKFCNQLEIFSAILAGLGHDLNHKGVNNMYKIKKSKKFNILTSEIAVLENMHCATFFNIIQLNRKHDFFQYMSNDEEKNLAKRLIITSILATDMSKHAKLLAKLQKRVECSKQFDQGQKNDLIDMQRFSNDRLEDRIFILNIMVHACDISNPTMKFNNYMNWSYLLTQEFNDQTIKEAKIGVDVTGFLIYKDKPTYYGGQMFFSKSLVLPLWVQIGELYPELKYLSEEINKNLEILQQKLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M7I6</accession>
    <name>A0A8S1M7I6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0550055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M7I6"/>
    <dbReference type="OMA" id="ISAMQKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="251" mass="28644" checksum="9E489F8DC7F32159" modified="2022-10-12" version="1">MNSSIKTEIGRYTDINHIFETVQKRGYLTQLTTFLEEVFTNEINFASQRLKEVISAMQKQGQKVEYSIQLNRNIQIFKSNFDLILTATSNNVWNQIVNSMNLISDETFQLNNNKSEIISPISRKIERQSEPDLKKMAMSQSYVSRSPSKISTIKQQQTIPLSEMSIQMRGSPTTFNKAKRQLDNSLTTSSPGVGRYRTEVAEKHVRETSPNATIGKSAKISWIDEKLQKEDAHSPGPIYNPVKTFCSKRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M819</accession>
    <name>A0A8S1M819_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0500018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M819"/>
    <dbReference type="OMA" id="LTYCCAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="264"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="553" mass="66644" checksum="B9DE2953C20572DD" modified="2022-10-12" version="1">MYECKGLFYWFQKEEAQNLLNINSKHQNELLAHQIFRIIEQIHIHEKQYQSSYFNQRCRWSCSNTGTLYWTHLFINNRAEYNKTILTRLFDRVQDRYYRIDLLLALGMQVINYLLIVEWNPRQSQRNSQVRIFMRFKSLFYILFKKLQFIKRKSSLIFQQIMNCKSKHFLFSTDYKLYLNPKYIVIGKVRMNFILKSEQNLKYKIPLNLSTIIKWIFNGDSTFNGFQFQWKNSWKTILLTHDQTLTLKDNLDGKVTYSNLAKMYNLIHCERSGYSSKVYLTYCCANQKKYYMQIFPISDLTNQEYIRLIQIINITHPNILKCEEYFVEEKNLYIVTNILNGRTLKERIGSQYRMEPQEIISIMKTLLKFINDMHKQGYVFREITSTNIFLQSDGEILLIDFELLVKIEEAFRTKAVSKEKDFMHEFKDSTKNTHEDWVEMERFCYEQHDVLILGYLLKEMLTSKVQQLHSQERARQLTINKTILIHKSPKYYLCQLLQRMLEPDPKGRITIQNAMYFLEDIDFDDNSYEDFPDTSEEQQFQPVKSISIKTFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M890</accession>
    <name>A0A8S1M890_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0520059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M890"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="155" mass="18457" checksum="883A69C0C92FC62F" modified="2022-10-12" version="1">MGYFCKKSNEESYKLFRKLCQLIDCTLMDIQTLQYIPRTIIASYMYLIISFQLNVFDIDMLEVMSRTSMFLLNRDNQFNQIFEQFVSITFGFALHDILPAIQYTVGFYELQINYETPPGVALLLNTPLESNYEEFLSFQCYSKYLLEFIRQKTRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9M2</accession>
    <name>A0A8S1M9M2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide methionine sulphoxide reductase MsrA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9M2"/>
    <dbReference type="OMA" id="FWCLEHD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774:SF1">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE MSRA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="1">
        <location>
            <begin position="6"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="181" mass="21054" checksum="07232F06758833AB" modified="2022-10-12" version="1">MTTINKATLGGGCFWCIEAVFKRIKGVNEVYSGYAGGANKNTANYKDVCRGDSGHAEVVQITYDESKLKYYDLLTVFFNSHDPTTLNRQGNDQGHQYRSIIFYHNEQQKLIAMDLIKELSKSYKNPIVTEIVQFQEFYKAENYHQNYYYNNKNEGYCKVVIKPKLEKIVKLFKDKLNEEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9P2</accession>
    <name>A0A8S1M9P2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase</fullName>
            <ecNumber evidence="6">4.1.1.130</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Coenzyme Q biosynthesis protein 4 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0480165</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Lyase that catalyzes the C1-decarboxylation of 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid into 2-methoxy-6-(all-trans-polyprenyl)phenol during ubiquinone biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + H(+) = a 2-methoxy-6-(all-trans-polyprenyl)phenol + CO2</text>
            <dbReference type="Rhea" id="RHEA:81179"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="4.1.1.130"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of a multi-subunit COQ enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion inner membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the COQ4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.130" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9P2"/>
    <dbReference type="OMA" id="WFEMINT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120539">
        <property type="term" value="F:4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03111">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007715">
        <property type="entry name" value="Coq4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027540">
        <property type="entry name" value="Coq4_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05019">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8072014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="223" mass="26304" checksum="E270970A7B17A252" modified="2022-10-12" version="1">MLRDLINNHTPLKNNFERLSTLFQCAINAYKYPLNGNHLSQLGELSGFYCVKDIHQRMLQHPVGQRILNDKPRVTPETFKIEELLKLDENTFGYQYGKFMRDRDFSSGERPIVKYIPDLELAYVYQRYKEIHDFIHVLLMYDVSVYDEIVVKWFEMAQLGLPSATLSAFIGSFKLNCQEKQKLIETLPSILKRANKSEFIMNVYFEEHINTDITQLRKSLRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9Y9</accession>
    <name>A0A8S1M9Y9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0530018</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9Y9"/>
    <dbReference type="OMA" id="SRYPAFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051696">
        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF21">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="2">
        <location>
            <begin position="20"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="585"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="744" mass="88266" checksum="297109A62D0CCC4E" modified="2022-10-12" version="1">MEKLGNLFEFFVTAGIKTNDQDVILQSCTTANKKDLAILTIFPNPVERKMNHVLADIAMWCFPLGVKTIKEGDNRFSKIEEYFKEDDVQASTNEINYFVINDFKQHIKFYCTSACFYEKMFLIMPPNDKLMQVFVPKAFCVISRYPAFIPQRSFLMQLLKIQKLRLSQNRERDKIITIKRGQQEYQIEEIKLYDFYIRTALTQCPIVQDNVEYRLKFSSSEIWMNYYVSNSHLTTHFSIFKLSNNLFDVLIFNQPIFNLYKLLLYLMLEYQIIIVSRKPAGITLFCQSLVELLAPLQWRGLFIPYLRPGSFDFHKSQLPYIIGLDRNLYEISEKPLPKELKRVIYDIDDGRILSRSDDPKCPKQYQNYFIRKMNTVFADLKFYENEIFEEGIMKYRQAFYNFKLLMLNDIYKYFSTDTSNQKSEKKVVMFDYKKFLTSFENQDQLFFLQFLKTLSFQQFVKDLYKVMEYREEFIKNNIQNVEYYFSSVLQFFDDVRFLSIRFGEQTLDDKLVILENLQNNYIKAVLERQNESSNYKVVEMKTTDFIPEYLEHLKNSSLNLSMEMQALAKFIQTAPQQEEIIVEQQQKIIQQGGPDMNQSEEQDNISQQSSKIQSDKSDQHSQFHHSLPPQEKVNWELQTIAVSNASEYRKQYQIKHLNLINKRNTFKRKNLLENEEEEEQMQLNQNTNTNNFIVTNIADKNMLSLPVKDNQSMAARSYFNKSMVPLIGDYGNAEIMVPNQDNMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCL6</accession>
    <name>A0A8S1MCL6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0590093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCL6"/>
    <dbReference type="OMA" id="RIIDKMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="348" mass="42101" checksum="30B5EF6F3D3E4675" modified="2022-10-12" version="1">MNDMTSILNLKREQFHVHIRKQRNEKQFKEQRKHYFLSLFLQQKQMQEISAKIRDIMESGEQLCGEMLIMIVEKIEDVLERESNLKLLDTIDAAIWMLKSVDAQIYEDELSDNYKMHRIIDKMIPLSQGYSIHPTHMNQQIIIYAAKFLKYWTLMDDKLIYNYYGEVAETVYFLLNKQEMIFIKRGVEIMLNLFECDDGRLLSKLHEVLICGQNLVKPICILLQNNLNSDIQTTLFKIVLQTFLMQDRNGMNQYLNYSNQENKQQFFDILIELVQESEENSRKLFRYQLHLIHLILEYSQNERELLYEYYKQKMNSSEFRIKMKDQWLFHEWNSIQNIVHQIQIHLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MMU5</accession>
    <name>A0A8S1MMU5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0660180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MMU5"/>
    <dbReference type="OMA" id="NIMSRFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8081808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="546" mass="64579" checksum="BCE50A54DAE83B6E" modified="2022-10-12" version="1">MKLFKAFCQGKSILVRDTQKLTKYIDLCLKTPVSYKGIQKSIGYDEIIRAQFNSKEFNKALFPQFINLIGRDFKELNQKEFNYFIQSLSCYVEGATNQKVYDKQIFENVVNQISLNATYVQKSLFFLTLYWYVAFYKAIYQSQQVVDKFQADLFKQVPETAEDRLWRVLIEMHQLQMKKKSQVKDETLKKEEQKIMEKFIDQIEEVDINLPKFTNIMSRFMLLKYYSILKYFNVQNYDKRFHNLLFSVYTTSQNYITENLSSLNSFGLYGYIDLISKNKDIFQATKWDIILNEINSKNFNEIPIFDIIALSFVIINNDKMTTQLSNRIIQHLYDNINSISSNNYADVFQILAQVDCDLNDKFVTKLQSKVIPKDLSLEHISIILTVLQRFHRIDLNILEKYLVGIGTRIKDIDKNTGLGILSALNYKHQQNPVIFSQILQLKLEEDKDLQLQIEILKYLQLHDPQNEICMKIQQNFVKNLKKEYDFKLICSILEVLLHFNDQSQKTITIIPELKQYVKKGLEQIKQSNELDKDVLEFLMKQYKIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNJ4</accession>
    <name>A0A8S1MNJ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0680050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNJ4"/>
    <dbReference type="OMA" id="IANYETH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="43343" checksum="AF8EA39C4E087A1B" modified="2022-10-12" version="1">MMNIQQYQNYFPYVNEAQVQFQGEIEQAKQNQGSNYNEFNLFQQNFYASTFPPYQFEFVQEHEIPSQIKNKKNKKHKNNDQKGLLPKTLESDKQFIEDQQQIIIPQDQIIKQPYPVFHYTKGNPMPKLQSDIVSNPHQDGQGSQEGSQQTQKQKGQHSQIKKPKKENINTGHWSTDEHSTYISFLQQYEDIMTSSMMKKTSKIFKQMSELIGTRTPSQCRSHHQKFNPYALRGENGKRLPRAERSRAGRKKKNPQTDLPKAEEANIIANYETHDPYYQYMMLEQQKFYYSNLQQEFWNPQHEMEHHPNIKKEEEIEFMNNPHIQQLQQEYEECLKYNIPYIQRNGIQSDYNFDARNQAINDLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTQ3</accession>
    <name>A0A8S1MTQ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0630120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTQ3"/>
    <dbReference type="OMA" id="MQVIGQG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="110"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="473" mass="54770" checksum="46613637CC243A8B" modified="2022-10-12" version="1">MFPKSCDELYQCRLFKKKFLGNSPRYFYIFLDKAVVNKESNRKRADRVFNVENTKRIVWKYTIEQPIKLKGIIFDNGNEQQEYFGDDDVLRELKKNISKLFFQAKVQEEYVAMQVIGQGNYALVLELQHLHSNQKFASKCIDKKKLQTIEQGMESVLNEIQIMRILSPHEQIINLIEVYEGDNNIYLIMDLAQGGSLYKEMKNKLTLYSRKEVQKIMYQILSGLQYIHSKGIMHRDLKPENILFKEKGNINTLTIADFGLSVKVDSYPYLYPKCGTPGFVAPEIVNLIDKTSSYTTACDIFSAGVIFHILLLGEGLFIGNGHQEILRMNKQFQVDLTRNKYQQLDSDAKDLLFKMIVQDADQRFTANQCLNHVYFSNEVAPQLQLKIQESPTKVSFDHYLDTYNSPDQKLMNKDGKKLSIVTRTPIYAPKASTPELPKQRSIMEELSPLSSFSLDQLNNETQKEDQFQFAITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZ95</accession>
    <name>A0A8S1MZ95_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0680144</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZ95"/>
    <dbReference type="OMA" id="DNVASKW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000983">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="180"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8082756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="208" mass="23300" checksum="01AA4EAB2173CB6A" modified="2022-10-12" version="1">MQGSQESVQIKLVVVGDGSVGKTCILLSYTTDKFPTEYVPTVFDNYTTQLTVDNQMVNLSLWDTAGQETYNRLRTLSYGSADIFLIVFSVADSSSFDNVLTKWYPELNQDELQKVPKIIVGNKIDMRDESNSKHIKKASAEQVLANQNLQYYECSALTQEGLKVVFDEAVKKALQAKSMNKPVTKTASQNKEINNGNQKKDEPCCSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4F0</accession>
    <name>A0A8S1N4F0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0770153</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4F0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000737">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001382">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="10"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="296"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8086902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="43045" checksum="DFD11D736C173C0D" modified="2022-10-12" version="1">MIKIQNKYSYSEEDLIGEGAYSKVYKGLFDKTNEFVAVKVIDWSKVKDKYYISGFQREVRILRDLDHENIVKIIDMIHDPCLNREYLIMEYCDSQNLTNFINQSGGLLNENVSKIVLQQLLTALNELIKKEYLHRDIKPENILIHKRTLKLADFGFSVKADYSGNQLFRENVGTPLYMAPQILENKHYSVKSDIWSIGVMTYQMIFGEYPWVADDPAQLLKSIKSQKLYFPSHISISDDFKEFIKKCLQYEEKDRYNWDDLIEHKIFKNKYVIQKNYTKIEHLQESINQIRQLQKKRGTPLEQLFTNLDMDGDKKLTFEEFQVLLLYVDNRMEINIQKGIFYRYNVSKNNLLNFNEFSRIFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDM7</accession>
    <name>A0A8S1NDM7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0860191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDM7"/>
    <dbReference type="OMA" id="IHEANHP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="230"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8090750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="516" mass="58358" checksum="33F726FB4FAD54FB" modified="2022-10-12" version="1">MINSVLETRGAHRIKLGIIAFSVITISLSIVFLVNMITPYGRLTKSSPASTFAQLILCIFVQMSLIFRLNDIMYQKKQPQYKLGLEIIIAVIAILSFIDFICWFINPVANVLAAFICLFTFIIAGFQSTLWILQRIQSSKGEQEFDDNLTQQISQNENSIRKTSKCIITLEYILYSFIFVLVILNTCQIIHEANHPGSLGEGEEYYTVQSQGQDVRIRTYCLGEKNLPQIILESGGGSSGIDFYEIQTQLSTNYRICSYDRAGYGMSWQAAAPQSSENTMIIAQQVMDKVGFNTSVPNSIVCIGHSVGGQLCRYYAQYMTSIQGIILLDSVPVMNWFYLVGQCQNQTVNQVYAQQQSTLPLIKAMASLWPFYIETPFFMSSGGFEPPNLQSWVNWQITTTRNWYSQSLGYESELEECQEQCMESSIINPQSMINKPIIVITATNQSITCADRNLTGTNCQNYYCSQNASFELAVNQSLLGNQVSTYVECPGICNHDFVWKQPDFIVQQIEYYIPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEF4</accession>
    <name>A0A8S1NEF4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0800197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEF4"/>
    <dbReference type="OMA" id="EPRANNN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="235" mass="27489" checksum="8C71AE270E130A70" modified="2022-10-12" version="1">MDHELQQLIDEQNKDEFSDDQDLVSQMDIEDFEQRLHTSQSFVREKPPQHNQRAQSGMTQTGKPQPQSLKDLNEKMQQNLAKKQVNQQTRVQGQQRSQQQFSQTQQVQKKQDKRKSVEPRANNNPRQVLEQAQRDNQELLQKLGVMKRQLQSLEAENAELKQEIQRKGNSSGFLPKIEQAGNSELKLLRMENEELKRIIQKQNGSDSGKMLQMYGLQVVKLKCRIEELTQQLEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFH9</accession>
    <name>A0A8S1NFH9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0790150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFH9"/>
    <dbReference type="OMA" id="CLIEDHS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="394" mass="45925" checksum="9F3CBBCDBB572E02" modified="2022-10-12" version="1">MQYCSKHKDQQIDLICLESACKSHLACVQCLIEDHSDHPEQCLFIDDILNNNIKLENSKVIDFITKLRSKQDLISVVDREFVQIQNEILLKFDTFQEDLQVYYNQLTPKEIQQKEIEFFDLLKNVTNCQEIVKLYQIYQNGDINKVKLNQELNAIINKPEIEQSKQQIFTIYKCLLQQSLKLQKKDLNQIQNDILETLQEIPKLLSLEKSIQFYSLQGNKSTIENGNIITITSQDQQVVIVSQNTFSYPNKYQFTIQIEQYDTSQNQQLQVGFAHHSKLMNYNYFFRQNSSAYFGLSNTGRINFSVQKPEGKIIGESVRQALKEKRPAHITLSLDIQNKQCLLQYEDIKIEYSKKKCSKLGFGGETGAEQFFQKNKKFVLFFIGNLADMKCKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGT2</accession>
    <name>A0A8S1NGT2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0900115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGT2"/>
    <dbReference type="OMA" id="DEMKHIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="321" mass="38126" checksum="8F60840D255FAFA3" modified="2022-10-12" version="1">MRQKHLVNELINIRDEMKHINNRIKTTSSIKNHEFISHQNTNYKIRKYCHQLIQIKPPQFFKYFISEYFLLDLLEKSDYEKILKITLKFIVKHFQDQELYQKYDNQYQSNEKNAEKINKIIKEVVSPIKSKIESQTQFYDQQLKINHRSCQTQPCYSESQQVIQFNLGLRQQSSIISSIQQDNLNSPKFNENTINAIDEISFEKSCIKKGSENQIGNHTKQNQLENNEKKHPYSVSLLSTPKQNESDNKKQGIFNNNNITVRETKRKEKHKTSLEFLTYDKQEEINKIKKQLPSTMLSLDFIKQRKKIPESKKGSNILSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NI12</accession>
    <name>A0A8S1NI12_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0900167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NI12"/>
    <dbReference type="OMA" id="NAQFCII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="229"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="289"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="466"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="532"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="610" mass="73105" checksum="2D6297537A1EA698" modified="2022-10-12" version="1">MKQEEDEKLFIDTVQTQQLEIESLKKQIRLQEFQSKQQHLELINSLDQLKKRNEELDIELNKQIESTHETQLQQFALQMQDQLAKAEEQILIYQENELKLIKEIQQLQGKLESDKINDLKQQLVKMEETAIALIVEKEFIISEQAKEIELLKQNEKFNNQQIENNNQILDIQELENKIQDYQYKIEHQQSIHEEQLSNLERIITNRIFESLELEMNQKKLVEQDLLHQIHILEEKNKYYEKLLNEQERKLNIFREEKNQEQTLYDKKYQEISERLRYALQQIIKSDKKCEDAYKQFSQLQREKQNMTKQKEELIRKQVYLENQNRKHTQVIQEYKELIDTLQQQVNEQDQIIQEQNEQLQKLSIHYEQQLKIAIQSHQQKFEFNDNEIISNQNELLSDLLKDSQISQNNQNTHIDSPTHSISQTHTSHLSDSYCSFQTKNSSRRRVGNDSKYDNIKDALLDAKHTIKIQNLEISNLEQTVEILQKELKSQLLEIHNLRKTKLDFKQDKDYKLEIEKLKQCLNTMETHYLTTKLSLAEEINFLKDELKRAESEALRSKLQLTQLNAQFCIIQQKYQKLDKQQQQSLTNRQIQNIDEKSPVKGRKSIFSIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKW7</accession>
    <name>A0A8S1NKW7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0910129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKW7"/>
    <dbReference type="OMA" id="TQNLFVF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="73" mass="8666" checksum="8177EEAFAE2F7516" modified="2022-10-12" version="1">MKTEQNDTKILNKSEENQQSIEEPRTVLINQPLNFVQTQNLFVFEFKSDTLSLIDLLRFKHSLAKKIIKKFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPF6</accession>
    <name>A0A8S1NPF6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0950131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPF6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001434">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="230"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="275"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="318"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="494" mass="57619" checksum="9FC8B2E1136F2E71" modified="2022-10-12" version="1">MLIRCTQADHLNQQIIGVCIDSQCQYQRPYCNSCLASHGQHLKKLTPLELLDEWIRKRVIIVQNVQQKAQECKLTLDSLLNKFINLCNINLAQLPQLGLQQIDNIVKCLSLIELSEKTLLNQFLQSIEQIKQIVKEILENQKKQINLRSTDNLQILEPLEIKEQIFKQKKPLNILKQFNFELMNQNSIKQENYCFAIAINKDNSIVVAGCGKQIKVFDLKQGKLNQSQLLCDHQFDVSTLNFMKKSNHFVSGSVDKSIIIWQMNQNNLWICQQKLVGHTRSISTLLLNNNENIIISGSNDSSIKFWNKQNGWQCQQTIQDHTSQVYSLNLNEKQDKLISCSYDSQIIIYEYTYIDQIWTVIQKIKAEQYGFRLSFINDNQFTFQPSNQEQMHLYEINSKNQKFEKTQQIIVKSGSCGCTNLFPQQYLKSKCLLVNKNGQNINLIRKKENGDFVTELTIEFDQFYIYGQLSDDGEYLITWDEKSKEIQIRKCKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NRA8</accession>
    <name>A0A8S1NRA8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MRH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0910097</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NRA8"/>
    <dbReference type="OMA" id="KSPAGCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071:SF13">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035872973" description="MRH domain-containing protein" evidence="5">
        <location>
            <begin position="16"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="6">
        <location>
            <begin position="14"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="194"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8092726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="227" mass="25538" checksum="A350E07420D2FA3B" modified="2022-10-12" version="1">MISLILVLQLTTILGCEFKQEDHYYNLTKLQRTEPYIVKASADSSFFKMAYQFNFCGNHPKHCQGQSGAYQALEVMGILTDSCEILGKPENQQISLIENGLQITYSQGSQCENNQKKSVNFNILCSPEADQNFALLSEDNCHTAFQLKSPAGCKLQYSTQPLYIYYLSGIGILLMILGACFGYKQLQKKKDEKSDQYEKLNSDQPQIQNKNPDQSTTVVINQEAPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P057</accession>
    <name>A0A8S1P057_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diphthine methyl ester synthase</fullName>
            <ecNumber evidence="4">2.1.1.314</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1020033</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 4 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="EC" id="2.1.1.314"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the diphthine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.314" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P057"/>
    <dbReference type="OMA" id="HNASIMS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141133">
        <property type="term" value="F:diphthine methyl ester synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000004">
        <property type="entry name" value="Diphthine synthase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000004">
        <property type="entry name" value="Putative diphthine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8096651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="278" mass="31651" checksum="B5AB2FAB357B7C06" modified="2022-10-12" version="1">MVFYIIGLGLGDHQDITIKGLEAVKTCKEIYLESYTSILGINKQKLQDFYGKEVIEADRECCETGIDRILENVSTDPQNNYAFLVVGDPFCATTHTDLFLRAIKLGIKVEVIHNASIINAIGCTGLQVYRFGETVSVPFFTEKWRPYSFYPKIKANLDHNLHTLVLLDIKVKEISEENLARGKKIYEAPRFMSTQIAVEQIIESDKQLGLNAIDEKTKCFGVARVGFESQKIISGFLHEFLEIDMGAPLHSFVICAKELHPIEEEMFQFYRNNQQKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P2H4</accession>
    <name>A0A8S1P2H4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1000075</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P2H4"/>
    <dbReference type="OMA" id="LIDWSIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="124"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8096064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="479" mass="55348" checksum="33C84B8A6DC1741B" modified="2022-10-12" version="1">MIDNSNTVKTSNFLLQSNRTQLWTLSTKPIDSSVSGCFHVSYKLGVFREASLYLQGQNLYKCKNHKWKVCDIGNSQLEVVRHNVFGEGCKLGFGQNYIEIYGKIEELLEKLKKQCIQSNFTQKYSILKLIGQGTYGKVYRVKNKSNQKEFAVKTFEKSLLILPSDKIALAKELEIARLFEHPNLLQFYETFESEQFIFVVFELLLGGNLRQEIKKSKISEKRAFNIIRQLLVALDHMHSLGVIHRDIKPENIMFRNSEELVLTDFGLADYYRKDGQYLFTNCGTPGYCAPELLQNKLYDFKVDVFSAGIVLFQMLTGENPFDSDDYNKRVKLNKQGLIDWSIVNVNGDALDFLQSLLSRNPFHRLTAAQAINHKIFNQDYGRFQRSGVSNTLVSTGSQLSSPNTKQLQIIPSPLQSPKINSLHQFEFDELDNLILEQSKKDKSIVNYPMLERKQNQQFQFNYKVQHRTTKSEVLLFFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P4H4</accession>
    <name>A0A8S1P4H4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1050100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P4H4"/>
    <dbReference type="OMA" id="IMNRNEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="360" mass="42337" checksum="9DEBF4C6B14AC5AA" modified="2022-10-12" version="1">MGQEFYNMQNIKRQKSIFDQTFSQRATTTAESKRTCKIPIQIIDFKNKFETYLSGQTSCVDVIKYNNSLPTKKYRNSILTKRKTLHITNTITQQETMIDYNDIKEFIVNHDQNYTEIKKTLNTQPSLPERKSSKQGFRTYIKSDYQENKQLGHLNLIAEDYRSQIRNNNLVSSPDQLIFKNKIKARTKHMALIKPKLDSANMQSRIKQLKLENGTKQPIVDVYQKLFRATSDHFLDALKLNCNVRTTELNKMGELDFYELDNRQKAISERLPKKSIYGNQKQLKYNKNCPTNQFDDCILDESIISETEAKLNIFYGQSKDYQKKIMNRNENPNKVVNIISKIDQIRDNIYKVNKNKLYCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5Y1</accession>
    <name>A0A8S1P5Y1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1070042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5Y1"/>
    <dbReference type="OMA" id="DQTFCTT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40515">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40515:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="114"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="404" mass="48479" checksum="CDA4C9715428A234" modified="2022-10-12" version="1">MQMDLYKERQQSNENSYQFKAVEAFQSLIQYMNELTDIMNKIKLKNEAEFINAYHNHMQKIKEELKELRNKIEEQQGIQNQNKIKVTSGDNELVYFREECLKLYQKVELKNKEIYELKYKLGELQKTNEFLEQQIKSLIKKVKQTEIDKELLPNIQLDQTFCTTTPAKQYKSHKRRLTDYQSKVELSSSLSKHHYSSIKEMFPTDELQKDIVKYEDFLDKITKYITIIEQKYQNQNRILNEKVQKLLISQNKKSIIRSDLEYFFLECVETVRKEIQKKRPLTNFQQYQSYLDSMQDFSQFYKEDKIKILELVVSNEKLLVFIYQKLFPNHVNLVLQSIKEDTHLKSLLDQNPKNSILNNDEIVNLNQLVTNYKVEVPKTTREVRSVSLNKQLEVRKGKLLFKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6A2</accession>
    <name>A0A8S1P6A2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1070081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6A2"/>
    <dbReference type="OMA" id="AYSYMQD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="205" mass="23855" checksum="693B1DE7543111B8" modified="2022-10-12" version="1">MIYKIINKFSVYIDPSKFPNGDLLSIYKSTEGKSIKLFNVFYLGLIGYNAYSYMQDKQNSQYALNDLTAASYKSGMENSIIIMAALGIGMIFFYWKTNKTLFNLWLSPNGKSIVMDSYSLFGLNSRSFYIDIENFRGFSYFLHPSLRIPMFKYKLGSTTRTMFFKHQYVLDEQIMKSVLQGQDIQVRPKGMEKELSSNQKKKYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7K8</accession>
    <name>A0A8S1P7K8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1080143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8099050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7K8"/>
    <dbReference type="OMA" id="KQIADCY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035713739" evidence="1">
        <location>
            <begin position="17"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="101" mass="11390" checksum="C66A45FBB850E84A" modified="2022-10-12" version="1">MIKFLLFITIISLVFSIQETTTHSDCLNQYCSAEVEACRKDQTCKKQISNCYQTAMDDLSPAGIQRSKAAYDLCLSTNSYAKVQNLAKCKQRNCPNKLLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8E1</accession>
    <name>A0A8S1P8E1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1090003</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8E1"/>
    <dbReference type="OMA" id="IAKPYSC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="142"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8099151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="185" mass="21374" checksum="ED1F61703E9C0F4A" modified="2022-10-12" version="1">MKKKSKKNHKKKHHSSSSSSSSKSSKSRSRSRSESKSQKSKSNQFNKEKTIDSSIQDQSTTRPYKVNKFALGSMLFENRRTNDRMDKSEVQNANTLLKKIDKSGRDAVLLSAAINTVQQDNKREEYQIELVNGQYIKIAKPYSCGFSNCQMRFVTTEELTKHLKEHDRVQSMKNTQRAQKFMENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PFF6</accession>
    <name>A0A8S1PFF6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1160096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PFF6"/>
    <dbReference type="OMA" id="HIYENGC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="334" mass="39661" checksum="120C0870530B55BC" modified="2022-10-12" version="1">MESEQMCKNHPKQSLSWICLERNCQQRVMCSTCAVKLHDRNHKLEELYNIYENGCLATYFSNKKNSTAGLLQLVNGEQDYVFENEQYDDYLQEGIERCLRNVSKKIEENKTQLIQQHEQLKEDKENERINFIAQLSELDGQLKSSLNLETDIENAFQQIELFAQKRELEMSNNKESAILLKKIRLLELMKDNFLKMIKSYFHNQFTAEFCHLFDMQIETLNAREVDDVQGFLDYVDSKWKQDYRNIQLSIEKFVGRLNEGLKYCIGMPLENQNKQYIVRSPMRQFPINIQTPAYHQFTSPKQLEFTKSQVIYEEPSQRKNIKIEPVSQSLQQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKB6</accession>
    <name>A0A8S1PKB6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1210161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKB6"/>
    <dbReference type="OMA" id="VPQHRKT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000093">
        <property type="entry name" value="NLI interacting factor-like phosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="33"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8103665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="276" mass="32401" checksum="C126DEF83C74CF3E" modified="2022-10-12" version="1">MNCLQYIKKCYENLKQKREGQEESYRLSTQESEGRKKKILVLDLDETLVHCEFKENQNFNYETILDVWHRGVLYNVYLCRRPYLREFLKQMSVYYEIIIFTAGYESYCDKVLQYIDVDKHISDYYARSNCIFVNGNCLKDLAILDRPLDQLIFIDNNPNAFELQPDNGLLIPSFLDSDEDECLLRLIPFLKFMANKSCVKPVNQCLKDYETIHGSLFSDSQITLQCHMDGEEGSLNEESCIIIDEVPQHRKTQTLMQNQSKEKKVQEARSQSLFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PM39</accession>
    <name>A0A8S1PM39_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1220141</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8104001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PM39"/>
    <dbReference type="OMA" id="ANILYEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="117"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="417"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="427"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8104001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="439" mass="51371" checksum="4C4675542B0964A0" modified="2022-10-12" version="1">MLIPTSSGHTSLANNANILYECIVERHHFFLNRKYYLQLTNQCLTLSQKQDQQNPKYIYQLKIDKVFTWSLQNNQIVGFQLPYQDKIKDFFGNPNDMVNLKSKMGALVCYQKIELFYKKEEILQTGSFGKVTKEICRFTSNRVAIKSIKCQNNQSPAIKNEIEILKRLKQTNTLNILEIKEVYKDEHNYYIVTEYLPGSNLKQHLERRNKPFLMQEALCIVESLLKGLQGIHQNGIIHRDLKPANLMYHNNQIKIIDFGLSCFNGKQLEQYPSCGTAGYSAPEVLNVWNKKQAYDYKVDIFSVGCILYQLLTLDGLFNSENEKATYRNNKKCQFTIKEKGSVFDLVQLFVKAEPQQRLDCSQAMEAVQALHDYKYFDVNTWYNNKVKYNQKGRSQCELNQEPQTKLSRQCSLTNFKYNEPQTQTQTERQRNQPKKIEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMI3</accession>
    <name>A0A8S1PMI3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1220102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMI3"/>
    <dbReference type="OMA" id="MFQYIPQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="20472" checksum="75FBF28F53A493FE" modified="2022-10-12" version="1">MNQQEYMNRIFQNQITNQPLLVCLQYQPLMFQYIPQMYCGELTQQNTEIKYVSNYCNGTINYEQQNQSNQQQPKLQEMPQSIKNETEREIKESKFNGHWSKQEHELYLEFVNNHFDILKSKYDKKSKKIFKLMSKFITTRTPTQCRSHHQKFNPLQSNKKKKNSRLGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PP13</accession>
    <name>A0A8S1PP13_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1240042</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="VIFQWSF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030991">
        <property type="term" value="C:intraciliary transport particle A"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000242">
        <property type="entry name" value="WD repeat domain 19, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057855">
        <property type="entry name" value="Beta-prop_WDR19_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056168">
        <property type="entry name" value="TPR_IF140/IFT172/WDR19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040379">
        <property type="entry name" value="WDR19/dyf-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039468">
        <property type="entry name" value="WDR19_WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14920">
        <property type="entry name" value="OSMOTIC AVOIDANCE ABNORMAL PROTEIN 1/WD REPEAT MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14920:SF0">
        <property type="entry name" value="WD REPEAT DOMAIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23389">
        <property type="entry name" value="Beta-prop_WDR19_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15911">
        <property type="entry name" value="Beta-prop_WDR19_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24762">
        <property type="entry name" value="TPR_IF140-IFT172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR19 first beta-propeller" evidence="7">
        <location>
            <begin position="22"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="WDR19 WD40 repeat" evidence="6">
        <location>
            <begin position="377"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="IF140/IFT172/WDR19 TPR" evidence="8">
        <location>
            <begin position="884"/>
            <end position="1110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8104509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1380" mass="158021" checksum="3C5CB3587D582CE0" modified="2022-10-12" version="1">MTEEKKNQIFEYKDDINGKVPVIFQWSFDFNFLVCGGDRSVVYVIDKRGKRLKDIQLPTNNKLLQLEWDKDSEFICIVQDGLNYVNLWAPFTNNPTLIQIELDNVKAKVSYARWSKSHGILAIGSDKGGVLFYTKKQAKKLPTMGKHSKKIISGDWNNEGYLITSGEDKFLTVSNYNAETIFESIPVKAEAKNVLWSISKTDSKEQIQRTVTAIIQQKTIIMFELGKNHKMPDEFVFENKYGKIIDYTIFNDGYLVIGFSEGYVVKVSIPIKDNKEDVRNKKLLKQVDSICTNDSLQKLAVAGDGFIKMVNLQSWEEITEEQIKLPNDCGRVSKMAWSSNGLILIVSTEKGNLLGYQTSIPTLTAIYKSMIALQQSFTQIKIYSTQDQSLTEKATIFLENEPNQISMSMKYFAAGLNNMVYYYNYLELKSKKDDTSPSSIILKMDYLGYVNQIILNNEFAAILSNNVCTFHKIEQAGDFIKQQFPPNDQEKPILHIGLTRHFLFLLDSQSKIRVYNFLEKQFIIEFNNDYNLTKIFPNFSGTRSICINNKGQGFLFETSLEILTKIPIFPEKTERIIWDQQDPNLFVTQEDILLTTYIVNKNNLKQEMVHPVLEYLSHEDLHKPTDPSVTKIEKGVQVLSLTNGTLKCFTTTRNVIGQPLLSHSYLNQYKGSNDANDGHFRYFLQNLALFKFANAHKAAVNLKNLKLFDTFGRKCLENLEFDVAQKAFQSANNISMVMMIQSFKHETEKTLLFAYVAMILGQHDLAQDLFLKSSYRLGALELRSDIQDYVNALSLARKIAPEQEPFICKRLGIQIETQGNNPEAVKMYEMAMLNEKLHDSKTVEIHNQQCVAGIARCSIKMGDILRGSSLSRQIQDPLILQEIALVCENMKFQVEAAELYEQAGCIEKAATIYITQKMFKQAAPLMNKVKSPKLLILYAKAKESEGAFKEAENVYEKAEDWENVVRLNLNQLNNIERAKFILRNKCKTVSLALMVAQYCERRGSKAEAVEFLILAEKKEQAFALAQNFNEMDSYVEHMKAFSLEERLQVAQYFEGKNQLDKAAFHYEKAQDPLKALRLYQQAGEEFINDMIDLVARNKQDNLIQNLSDYINGDTDDIPKDPIYSLKLQKAIGNLQAVGRIAITIAASEQECGKYKEAHQFLYETCDDLKSSGNQIPFDLYQKLMTLHSYTLVKKIMKLEEHEDLAKLLDRVCKSISQFPNGATNILTMAVIEATKANFKSLAYQWAITLMKQEYRSQINEKFKTKIENIARKPIKEEIPDKKTPCPFCGEFVSEFCLDCPKCSNNIPFCIASAKHIVAEQCCLCPECKFPANIQYFRRILEIEPICPLCQKQIRPQDLKEITKEEVLKLKRKAVVDEKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUA3</accession>
    <name>A0A8S1PUA3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1290140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PUA3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="90" mass="10624" checksum="AC66CD8B6A134D8B" modified="2022-10-12" version="1">MIQASKFDKLLIFIYYNCGRLIRKEVHLLSAAKLSIIVGGLYMRLQRLQCHIFSEGNFSNCWKNVQNRQQNVNCYKISSSFRIIFWLICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PVZ5</accession>
    <name>A0A8S1PVZ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1330087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PVZ5"/>
    <dbReference type="OMA" id="DMLILCH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="255" mass="30537" checksum="36321E687434186D" modified="2022-10-12" version="1">MSFERYHPSKLNIESSDNNYHHNGNNHVHLKYAPIKIIHNDIANCEERRKYPQLNLRFQPADETITRQDPTVTIPLSFLQQLIQQAYSNKQLRTLNNPSFRVEENRNDAVEELIKQIEIQQKGFYPIDEEKKIQNALIQNQMNFILKNEEAIQMIETYFSENTNLKPHFKTWLQQLEVKAIQDMLILCHSDPKGQQYQDLFKALFQKLSIEFYQKHAYAQILRSDQQEKLKYLSQIGEILNKITKPQEYYYFKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXT5</accession>
    <name>A0A8S1PXT5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1350155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8108060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXT5"/>
    <dbReference type="OMA" id="IREYHEE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="206"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="251"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8108060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="466" mass="54861" checksum="5BC81D5A1D88C7F5" modified="2022-10-12" version="1">MQKVSEQSLKEEYQKKVDQCLESLQQAVDKSFNKIVAQLDQLLNDETQFYNVIPTQLKDDLPKFNSSRLLKFQDDDFPKQIYQEVTLLIKSNIQNQVKDKEQYYQMEIQNNQIMWEQIIQQKDYQIQQLQNTQSTQTIQQPSTSLSNQSNLKPFTYQLIKENSIKQNEHCYAIAINKDCSMLVAGSQNQIKYFEFKQGVLKQVEILSEHENNVSTLNFMNKSDQFISGSDDKSIIIWTRNHKNSWICQQKLKEHTSSIRCLVLNNNEDLIVSGGLDKMIRFWQKQDEWLCYQTITDHTERVLGLSLSEQQNRVISCGYDNLILVIELSEQNKQWMVIQKITVESGYRLCFIDNNTFTFYPSWYECIHVFEINNANQQYIKTKDIQVKGGSDDFLFPSQYIQSKCILVNKNAQDVSLIRKKQNGEFIVEQSIQFGTRDIYGCMTDDGQYLITWDKKSNEFQIRQFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYQ4</accession>
    <name>A0A8S1PYQ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1370002</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYQ4"/>
    <dbReference type="OMA" id="FEENDQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008251">
        <property type="entry name" value="Chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01393">
        <property type="entry name" value="Chromo_shadow"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="4">
        <location>
            <begin position="82"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8108326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="144" mass="16673" checksum="6772613B6A47DCA6" modified="2022-10-12" version="1">MIEERMIKCEKQFPTILESSHNIQKNYPQAKQTKRGGGYLKKEEIKSEQVQSLAAQKKKSLQLRTSKCSQQDLKKGGQFGKNQPEKIISHTGHSLNEAKFLISWKHLGSTKPSDSYHPYSLIIQHNPEILLNYFEENDQYIIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q296</accession>
    <name>A0A8S1Q296_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1400060</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="5">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q296"/>
    <dbReference type="OMA" id="IFDIQRP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07834">
        <property type="entry name" value="STKc_MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000815">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000098">
        <property type="entry name" value="Mitogen-activated protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="41"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8109272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="427" mass="50071" checksum="D8BB0BB34D234FBD" modified="2022-10-12" version="1">MQQEFKLPQKIIQLYAKYQNKSQKVKKFQLQEHTFMLEDNYSPVEIIGSGAYGCVIQVDDKNAKVEKDRQVAIKKIERAFEHRLYAKRTLRELKILRLMKHENIVELKTLLLPKSREEFEDVYMVTELLETDLAQVIKSDQVLTDEHIQLFLYQILRGLKYLHTAGILHRDLKPRNLLLNRNCDLKICDFGLGRAMADPSSSNNANIMTYYVETRWYRAPELLVSFKNYTPAVDMWSVGCILAELLLRKPFLRGDSTKRQVKLIFELLGTPNEAYIQSFQDEKVQNNLRKVIKETGPKQGIPLEQLFKNASKNALDLLRKLLTFDYRQRITVQQALEHPYLAQLHFEADEPSAQLVNQLEFEFEKYEMTREQIKDLLYEEILLYHFPEFQASYEAKKKSGQSLISHVVNNENAKIFDPTADDDLDRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2P4</accession>
    <name>A0A8S1Q2P4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Poly [ADP-ribose] polymerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1410012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2P4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36649">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36649:SF28">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="51724" checksum="189162343CA77C45" modified="2022-10-12" version="1">MSITSQIFKQKSQNQSQSEIILQKKVIDDNFSYIYHHIQSEIRLKNKPIHEQIKYLQDDIQNLDKQNNNITQEEKKYKKSRLKIQLMSVLRQSEHVKSIIIPEINQRIEKDNTNIMLYVLKEAISRDQNREFLLTDGIHSCGFDSIIGVYGQDQQCLRFRTKEVEFKQLQLYDKLRDEYLFDFKEKVAKIYQIESSEVDILDITEGSTIISFKIRSKIDLENDKKSIEFLEKVCNGNVEFYNYFVTAQDKKRTEIGLELNDFNSKFNMTWDHLPEFEERGPFNHRYNYYFPKGCYGFGLNITKYGQDDNWIKMNGNQDEWRILFHGTSNSNVESIIKASLKPGTRQAYTNDDCIDEYGRTVKVGNGIYFCDNFMTCIKDGYANPIKVHEKNFSTIFMSRVNPKKIRQSLQMKEKHYFIVNNSEDVRPYRILMYEVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5D7</accession>
    <name>A0A8S1Q5D7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein eS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1440086</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5D7"/>
    <dbReference type="OMA" id="MCEIITR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03122">
        <property type="entry name" value="Ribosomal_eS1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_eS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018281">
        <property type="entry name" value="Ribosomal_eS1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027500">
        <property type="entry name" value="Ribosomal_eS1_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11830">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01191">
        <property type="entry name" value="RIBOSOMAL_S3AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="initiator methionine" description="Removed" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8110493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="271" mass="30814" checksum="F89A0E82E661E58B" modified="2022-10-12" version="1">MTQGKNKKLGKKKGQKKTIDPLARKEWFELTAPVPFSAGGFGYTCINKSAGTVVATEAIKGRVVEASLADLQGQSDQMAWRKVKLVIDDVEGTRCRTSFYGLDSTKDKIFGMIKKRQTLIETQVEARSSDGYILRIFVIAFTKSIKNQQRKTTYAQRSQIKDIRKKIVEIVLKEVSKKSITQLLNFFNQETLSKEIGKATRGIFPLQNITLRKVKLVKRPKVDAQKLREFYDDSQRSKGTIRRHGQAEDQTALNLIKQGQIDKEQAEQKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q6G8</accession>
    <name>A0A8S1Q6G8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1450067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q6G8"/>
    <dbReference type="OMA" id="CIPAIDG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40849">
        <property type="entry name" value="C2 CALCIUM-DEPENDENT MEMBRANE TARGETING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40849:SF2">
        <property type="entry name" value="RGS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="239"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8110747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="456" mass="52994" checksum="2A8DDC4E1CCF25B9" modified="2022-10-12" version="1">MDAIKNFANNLTNRDQNNNTNGKIVPLEEKDREPLSQKNPLGSMADELQMAASKGIQKLIQSVDNAKADVKQGFTQLDDKLKLMLKQYTHQINVWARSKVLSSVIIVIEKFRPTVKEGLKDPDMCQCVKNLVDDTVDATWPEIVDEVKYQLMLQIGDPYTEIEEKKQTLAFLYPFVWIRNWYLYSNYPFDRSVWRQYKTISYWFWYIVSLIPFYGVSQYFYVLDFIMIDKGDEYQLVKFILSFKTMFFITQGVMKALIGYFMLYACTTMNYIDNAYQDDCTRKGPGSSIEVFSEVIGQFVQVFLIWLAFFLLSCSKQKGKPIFQYDDTPQKIEGCFERGGRLKYFMFWDIFALGSSIGLFWLVYLTQDTRPNFDNSGDFIYFTEICYGMLSFPFLIFNVPFVNGRLTKAKATAYDRKGNCIPAIDGLRKKIKSNQEEETNLLLNTLTIDVGEVFDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8R3</accession>
    <name>A0A8S1Q8R3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1470110</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8R3"/>
    <dbReference type="OMA" id="IQLIHIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF147">
        <property type="entry name" value="PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="162"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8111427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="323" mass="38458" checksum="40C7014A77B71A36" modified="2022-10-12" version="1">MKYYKQGQFLCYIGTWIPFVILVTIFLYFYVVYVQTYLLPRIRSEYIERPVVEINTYLIQLIHINDYVFSNSTVVITILLHTLLILFLITLIRVVITLPGRVPQEWLNKVEGEINKMIENEENMINFHKKGSQTSTSFSSEIDDEQRLQLNIKARLELIDKSGHRFCKNCQSFKPKRCHHCRQCKTCWLKMDHHCQWLNNCIGYNNYKLFINLLCYSWLLISFIMITYSRCYYDTLNSFSSDSKLFLVSFTFLYCCFLWILLTAFTLFHLWAIKSNITTLEYCENKPRQPLQKSALENFVEVFGINPLIWFLPIQPNTKPILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDW8</accession>
    <name>A0A8S1QDW8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1570068</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000162">
        <property type="protein sequence ID" value="CAD8113942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDW8"/>
    <dbReference type="OMA" id="WKPTISI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23798">
        <property type="entry name" value="UBCc_UBE2I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000142">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="3">
        <location>
            <begin position="8"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="1">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8113942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="165" mass="19063" checksum="62D1B5335153B24A" modified="2022-10-12" version="1">MSKEVSELVITRLKAERRAWRTDHPHGFVAKPTTKEDGTVDLLKWVCEIPGPQGCPWEEGTYTLHMDFSHDYPMKPPKCQFRPVLPHPNVYPSGTVCLSILNEEEDWKSSITVKQILLGIQKLLKDEPNIESPAQYEPCNQYKNNRQEYLKKAREFAASLKKQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QK60</accession>
    <name>A0A8S1QK60_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1690003</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000178">
        <property type="protein sequence ID" value="CAD8116049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QK60"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="23"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="327"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="544"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8116049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="625" mass="74267" checksum="83D3E808A9D136A1" modified="2022-10-12" version="1">MQYHQLVNTLLDAQTEEYPKRRFHLTKLLGSGSKGGAFLASSSDWGNNPKQVVIKIQKNMKPNEKQFLSCLLEYQNLYENGNNQQYLPTYLIRIYENFQWQQNHCIVMEVGQEDLYRFINNSRNVSIQEKEKICFQIIYPILFLHKQQLIHRDIKPENYIKVGNVFKLIDFGLIRSSISENKTQQVGSTIFQAPEIIENSSSYTDKVDIWSLGCVFYEILATQPLFDGQTHQEVTKNIKNHKNYPVPVNQKINQLQISQQLKTILISMLIYDEKKRPSIQQVYDQFNSYLNPQPIVIPQIPLHLDQYITQCNDVIEKLIKDGQQQIKDTLTNQKSFYEGKINELKNEFQTQNKINQNKIDQQSGEINELIKQVENLNQQKKQLEYDQQNFKLEIERFKQKIKDEEVKFNTEKNQLNKEIKEFEEKINKMNQFSENLKKENENKNQELQKLFIKNQNNETLIQEIQDKLKESSTSLNKAKSLVDIFQNQEKVQKSQIQTQDQQSIIQEITYTQEQKSENPYLNISSSQEYNNLIENLRLEFQKQGKQYLDQITNLKKELKVLEEQQKNNKKSRIQFKQQIEQLKTPEKQVESLIERFKNKQNLQEFMKKNNKQYLELVQLLNENFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QN10</accession>
    <name>A0A8S1QN10_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Polyadenylate-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1670025</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000176">
        <property type="protein sequence ID" value="CAD8115927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QN10"/>
    <dbReference type="OMA" id="MIQDSAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="10"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="86"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="161"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="5">
        <location>
            <begin position="410"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="355"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="355"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8115927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="55932" checksum="0F51FF4315F76F8D" modified="2022-10-12" version="1">MNTTSIPAERTLFVSKYPQQTTTEQLKNIFSEFGQIEQIISQGSGVCIIYKENQSVTSALQKSININGEEPYVCQYLNIAQLDHEANVFIDNIDKKETQSSLRQLFSQFGTILSVKLQKSDKVGVAYIQFDKKESADKCIAEQSRLGFKIQKFTSKKQHRDQVYISNIKVLPNVTPDQLKSDLQSFVQSFGTVESLILNRNTTNGTFFAFVKFVNQESAKAAVKSNKLFNDQQIHIEWVVNNVDKDIEANLFTKNLKADITEEDLKKALNLLVTVEDIKLMPSKQPPNGNRQPTQIAYLYFKTAEDGKKLIAYAYDHPQIVSLYVNGVISLSPLLPPSEVNSLLQVKKNTFQRQFHQPQYQQYQQQQMPPPPYYQQQRYPPQQQQPPRQHHNGNRYPQQKRPYYQQQIPDLVQLQIMVDRGQKQQVTDMIGMQLFPKVTQVVGPINAPQVTGILLDFENYQLSDQVRMIQDSAYLGENIRQALEILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QN93</accession>
    <name>A0A8S1QN93_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1670070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000176">
        <property type="protein sequence ID" value="CAD8116017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QN93"/>
    <dbReference type="OMA" id="QVQCGLW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035815893" evidence="2">
        <location>
            <begin position="21"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8116017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="116" mass="11562" checksum="22E3FC9D54474033" modified="2022-10-12" version="1">MNKSLFASLLILLIIGQVSCDTLEDIKDSAIVSTLTTIGITVGAKVGLMTAGFSSIGPVAGSFAAASQAGVGNVVAGSIFSIAQSVAMTGVLGTVSLPASLIGVAAGTLYYFYIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMD0</accession>
    <name>A0A8S1JMD0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tubulin--tyrosine ligase-like protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0040498</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutamyl-[protein] + L-glutamate + ATP = gamma-L-glutamyl-L-glutamyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60144"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:143622"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:60145"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMD0"/>
    <dbReference type="OMA" id="FNMLIHT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF145">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00041448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8043274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="565" mass="66889" checksum="356065A6FA3C6306" modified="2022-10-12" version="1">MRQKLQLKGDISLSQSAIQRRNSNQRQKSPIQGRRLFQSHVIQTPLSQQYGQNQQQIQQSQQQQFIQGRSYSQSNVVFNEEVPQKWPIIQLPFFKNTEPTIFFQYPPVCEDKRPLVNARPMTEEEIKKYSLTCKVLGSAPYKCVLTSFDWAGFKRIDEDDEDEEWNVQWGIGNKTTLRNMNRYQKINHFPGCWNMGRKDLLWINLSKFKRKYPKEYNFIPNTYLLQYDYDRFIMAQESAQKDTLWIKKPCADSRGRGITMVSKKSKVKKDKNFLIMDYIYNPHLINGFKYDLRVYVLISCFDPLRVYMYKDGLVRFATQKYSTNSKDLTKRYIHLTNFAVNKLSPNFVKNQNTQKDDEGSKWSHHAYRKKLDELGIDSRELFNRIKDVVLKTCIATEPFMLDSNAKSQEHRNGYFELYGFDVLIDENLKPWVLEVNVSPSLNSSSPLDKKIKTSLISDVLHLVGVPLWDKNNQKISSPPKYNQRNIKEIPNINTSNCLNKLSQNDFNMLIHTVEEVKRRGKNFSCVFPNPKTIHQYMDLFEFPRYNNKLIERFYQDRCNWLSRFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP03</accession>
    <name>A0A8S1JP03_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PRP1 splicing factor N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0060275</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JP03"/>
    <dbReference type="OMA" id="DGWAWYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010491">
        <property type="entry name" value="PRP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06424">
        <property type="entry name" value="PRP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PRP1 splicing factor N-terminal" evidence="8">
        <location>
            <begin position="12"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="763"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8044193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="857" mass="98877" checksum="C4FCB7653F40FB85" modified="2022-10-12" version="1">MFQRQKDSGNQPTNYVPGRGRGAVGFITRSDIGPAKVDIGIEQDDDQNDYNDAKYDEWNGYSIPLFNMGDYDDEDREADLTYNSVDRKMEERRLKRMPNNLPEKPSIVNQFSDLKRELAKVGIDEWLSIPDIGDYSIKKKKQDKITPVPDSVIMSAQLSEKNLQTKAHDLNEIGEARGALLSLKLDKISDNVSGQSVVDKRGYLTSLASLPHLSENEIGDFKKARLLLKSVIQTDPKNSFGWIAAARIEELDGKLPAARSILAQGLQQAEDQEDIWIELSRLETPEKAKLILNKAIQTLPHSVKIWLNAVNKEQETQAKIKLLKRALEFIPNSVDIWKELVSLSSEQEALVLLYKAVECIPKNLSLWLALAKLETYENAKAILNRARQNLPQEPTIWINAAKLEESAGKDKQTIAIVLSKGIKILKKNQVKIVKEDWLQEAEIAEKCSNINTCYAIIKAITLEEDKQSDNSWKQDFTQFEQRGSLHTARAIIAVEAERQNNFEIWKEYIEFERRHKDEDTKYFDQALELAVSNLPQIEQFWIELILRKQELQQNYLEVFEKSPKSENILLTLSKLEKQQGNYEKAYQYLQYIQDNLIPSDKTWVKMFKLMLLMNKQIEQAEKILKQYPQSDKLWILCGQAKINQKDYQGARQIFEEAIKTLNNSLNVWLTYIKMECQQQLYTRARPLIDRAREKNPKSSQLWAQAIRLEIDAKNHKAAQFLLSKALQCCQLDGELWSLAIELEPKTTRKKKSADAVTLCVDNPFVYLSTAKVFLNEGKIEKAKRWLEKALILEPRLGDAWAYLYLIEKEILQQCISRRPKYGRLWKQAKETLNQKYDIEKILIEASKLAQKDIAKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP81</accession>
    <name>A0A8S1JP81_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050369</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JP81"/>
    <dbReference type="OMA" id="NLVYSIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="186" mass="21454" checksum="F7CF1CD99B4E5803" modified="2022-10-12" version="1">MEIDHELTPFNIKKLVNNGHTTRVLFESEQDTFICEIIGPSYQQSPECQINIKLDGNEILKRDIYNLLKHLIFTETYQKQSFTVIFDICPPYSKAFNALYYAVLLHGIRIKDSFTSCSAFLDQNKRLIVDRKEKQWSAKLNLVYSINLQQILCSDVIGQFNNDDLGLLISGCISGCNQIYNFIKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPL0</accession>
    <name>A0A8S1JPL0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prefoldin subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0060103</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="3 4">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPL0"/>
    <dbReference type="OMA" id="KFGRAIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23165">
        <property type="entry name" value="Prefoldin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000005">
        <property type="entry name" value="Prefoldin subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016661">
        <property type="entry name" value="PFDN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100:SF9">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016477">
        <property type="entry name" value="Prefoldin_subunit_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR016477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8044021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="127" mass="15096" checksum="20CD1E8DBAE07A02" modified="2022-10-12" version="1">MKFTSTDNIEVDRQDQQKINSFSRLNMQYHELIRLNLARKDELTRLSDSKDEMELMEDDEKIPFKFGDSFIRVNVEKARTLIDDQVIKIKKQFEEDTKKIEETLKSMNNLKAKLYAKFGSQINLDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQT4</accession>
    <name>A0A8S1JQT4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035948096" evidence="1">
        <location>
            <begin position="25"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="252" mass="29721" checksum="2CA0816A621D0F65" modified="2022-10-12" version="1">MLLLFRKILTGLFNLLSIIRCLRSTIVQYVAFNPPEVGYLIRNRNEVIKTQTMSPLQEERSKTLYEPHSPLIQVKQPKRQSHQFTKEHLAIISPRIKYYNTLQNVEYKQQLRNPKNQHQYPSQEFNFISNELKAMEIDNNPGLPISSYVLXICEKHVTDVTRKVISKKIVLCQELLVMQEDKKEIEKEEDHPLHRNLLHLHQNLEEEAEISKKIDKIEDNLGNHQVKVENQTLHKHQEAQGNQQTHYHSKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JR45</accession>
    <name>A0A8S1JR45_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JR45"/>
    <dbReference type="OMA" id="KTINCYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="405" mass="47969" checksum="D541E604D3087E8F" modified="2022-10-12" version="1">MNQDQTYQHDYTVDIKQLQIINREQALVWDTMIEQYQKKIIHLKKKLNIEKTQEIQRQNQLDKKQQLYQEQCLLAANSYQIDLFELRKKDLKKQFELKKAILKEQINENKKSYEQISQNLQPLPQEQPKECSFITGASSDYTLVLALQKKNYQKTFISLQSFYPECYISNSQDCENISVIAIKDISYKATKNSIQNKLLLANADNKTINCYMVSYDNNKLSKDLIFQQELQHNIDLIHITKTLQIIVISKLGGFIYLLNKNQKQLIYQGGHVIGESVYFENVDILRVSNQFKIITITNLLTNPEYDQLLTEQYSALIYYHTNLPCFHLMYIQHPQIINKELFVYVNKKNKVRVYDYITQQKKKFKVPTQNIQIEFLNYFDKTNKLVFCDSYGQTYKVEKFTNLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JV17</accession>
    <name>A0A8S1JV17_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JV17"/>
    <dbReference type="OMA" id="KENQDRD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="18"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="155"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="221" mass="26357" checksum="BE58666480D6943D" modified="2022-10-12" version="1">MQQQLKVCNNKENQDRDFSKVESRIQLLIDENQRLNQLLIPKMEEIQQLKNENNHQLMELNKFRGQDCEQKILTEIILQKTGDCDKHKRQLLKAEKSIDDLQQEINRLKFEKDQYTKQILEAQTQNLLLDQERLKQIDELKTKVRKFNQGSNDQIDQLKFEINKSQLENESLKVQLKQLQDENVSIKRHLGCLSGYILENEQLRKENENLNQNYQALLAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JV81</accession>
    <name>A0A8S1JV81_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0060409</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JV81"/>
    <dbReference type="OMA" id="MNIQDQS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653:SF1">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="2">
        <location>
            <begin position="7"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="415" mass="48068" checksum="E6A9F41B65A6A51B" modified="2022-10-12" version="1">MNYDYIGAIKIKDGLFLGDQFAAQDLEFIVTNKVSRIINCASKQIPNHWESIGIVYMSFPWLDNDQQVILQQDEIINNIMKFVDDALNNGESVIVLSVKGHNRSVATLCVYFMKKYRWTLYKTLQYMHNRRPDLEIRAHFFNQLLSVETRLQKQGYGAKTYNWDETYTQGDNDEIILRNTYLNSQAQGVAEFKDHDPKPKESKLKFAEKVSMYIPPYDKIVFSKTRSIPQEAKPIIKLSGSSKQIDPSFQQQTQKQQSINIQEQSIQQPQKDSQKSSLQQIQPQQPQRPQSQGAQQQRLTKSDSVKSGSVFDNNASQPQKVYMNNFMDSRDQFLQKSQQQNGTRRPQTAPNQLKAPRVQTTPYKRNTSSGQRQEAGSQPNSQFKPMRQRAQSPNSTYNTQNPYIQRQFKAKAWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZH5</accession>
    <name>A0A8S1JZH5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">XLF-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0100193</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the XRCC4-XLF family. XLF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZH5"/>
    <dbReference type="OMA" id="CNNYKTA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032807">
        <property type="term" value="C:DNA ligase IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052287">
        <property type="entry name" value="NHEJ_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015381">
        <property type="entry name" value="XLF-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32235">
        <property type="entry name" value="NON-HOMOLOGOUS END-JOINING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32235:SF1">
        <property type="entry name" value="NON-HOMOLOGOUS END-JOINING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09302">
        <property type="entry name" value="XLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XLF-like N-terminal" evidence="7">
        <location>
            <begin position="19"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="263"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="272"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8046120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="293" mass="34805" checksum="F8485911278BEC15" modified="2022-10-12" version="1">MNVEEEDLNFLKAQPLWTGCRNTPYLIRAGINIDKCLIFITNLRQSWICKLNKDRIQKDKELYNKSIQTTNFQGVLDLLDLALKYDPQAEYSFKLNESSLYYGYSNVIKIYTLTWVFDCKIVDDQFHNKILINDIVNPLLLTIQSLENRCNNYKTAFSQLEQDYVKRLNEKERAQYKVRDKDDDLSVYILDDQIDQEKICQINFTPIANYFIQYYAINKVNKTLQQLKKRQPQQQNKNDNTSQNLHKKEQLWQSYENTLSDMFEGKGQQIIENDKNEQKQDRSKNTKKKAGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0I8</accession>
    <name>A0A8S1K0I8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0I8"/>
    <dbReference type="OMA" id="NIDIVMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="1">
        <location>
            <begin position="173"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="611" mass="71091" checksum="6C6DA1AE18DC6058" modified="2022-10-12" version="1">MVFILTLEQAQISIKIDKPSYFPGDIIKGVIYIKIKTKDINAGIMNLKLCGQEKVNQLDQQNQFYSFKQFIYKKQKKITDFGKKMPIIACRKYFEIEVPNISPSFTINYYKMVQCRILYFLSVTISSEDEKYLYKPPKKHRIVIHILQKPQIQPNLSLEHIGESKITQRCCFSSGTTKVMIQLNKPSYVFGENIDIVMQVNNSQSNYHIQNFKFSISAQLVILYKQQKRKLSNYFDIFDQELIQQVDAGDQKEIKVTLNLPQGKPAKKYNSIFPQTSIKTKRIIFQYILTLKIIFGKRLFLSQEDLIIPIPLILIQNQKREKDNICQSMDNISMIGSVFETNQKISQSILKKFIFGDHNCSGYGEQDGYQGPERKYQPIESLDEKALKDLKGALEQLMFKKQYEIEGSDDEDKLIEEIDGVNLNNVSINIDQSEKQQQPKTIFQQNNKNDIFFPNQNIEEAKLDNGIQDQLQTIKEEQDSKGTQYNLNNALSSSQIRKNQQGEDNEEQQNLQFQQQSYPFEAYGSFQKVIVKNERQDQVKMNTEEKDNLKKPTILKQNRSLNNSIDQIDQKEQKQFKVQFNDATSVDTLDGKTGERLSTQCRKNETQLKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4J0</accession>
    <name>A0A8S1K4J0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4J0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="119" mass="14290" checksum="ABBD8A70B285F2EC" modified="2022-10-12" version="1">MSEENISQKNQFEFAKHANAVRRFENLEKLKLIERLETMQRIKNSNDRLEIKKFMFQLQQRMLDEPNQSSKANFRECAQRSVEKLKRDSKLSRSPDSRQTTAAEKYIEIKEIHVKPFKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K549</accession>
    <name>A0A8S1K549_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K549"/>
    <dbReference type="OMA" id="DAIVQNW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="420"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="454" mass="51665" checksum="E56A279E0D7A440F" modified="2022-10-12" version="1">MLAKQIFLAKAQNKPQQTGKIEFPTLLDLTIEQVALNFDLYSSLPGIPEKIKHQIISKAPKNLPVTITYNSIEDENYWKQACETKWKGTHRTINICKHSYSYKIAYMENFLEEYFKSIKSLESQSEKDELQAILKAVNNWVYSLNISQTQCNIDIGFLCQYLPCLQNLTVIYGVKTSGIDQTRKEILGMKLSQAAELGEAISKHCRNLQSLSLPSNLIDDDLLRLLMTGINLDISIIELNLSHNKIGDQGVIRIAKYLMRSEILLKLDLSNNAIGLVGSKNLAYALLFNKSLEHLNLSLNSFNDIAGANFFNKLSQNKSIQHLNLSANQLGSLTAEMLEVYLSDSSCTLQSLNISNNNFSENNDNKDKNIYEKLKTGLTKNTSLIHFDISHSKFKRINAEKELQDIIVQSRLKQRKIPFVSKEEFEQQQAQNKLRKEKLEKAKTKQVEEVISPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9T1</accession>
    <name>A0A8S1K9T1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9T1"/>
    <dbReference type="OMA" id="SHICIYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="68373" checksum="4663C0515D80BDB1" modified="2022-10-12" version="1">MLHKMFLTFIVPEIEQEYRLNYQPYMINEYKTLTTLILFVGTFIILSFISESNYYYALYGLICGFPVMISRYFVSKYPQFSNFVYPFLQITVTIIYNIFTLNQNQDDVPQMYYKYQWYYGFFAAISHICIYLQGTILCFQTITMIGILAWHISRFDFSSPNGAYITVQIFIVFVVVFFCRREQERNKRLSFLKYRENMKWFQIMDKIVKKWTMFVQFDKSQDQLFLVQQSKAAQSEFMIQNNQEFRSFLRNTQIEQTSSQKTQSGQITLEDILRQLLSTKICKFDTMNSLKDSLYYTYVGYQQKTGRYLKVKLVEYYLSDSKMIIMILQNEKQKNKEIWKKFLAVESYFRSLTNKSLRFIEKTTTAISFKYSNLHNLIDDYNYFNAAFIWLNASKYVENRNQVSINQVEDQLKKIFKKSIQINFKQEQCSFQSSFQVVMFICVNLIKYIQSQTKSKVTIKIFEQYQQQVRYLEFYFEAEDFKIKSNLVHLLQENKYYQKKKIQFDDQNFKRIMDSYINFLEIIDSNKSTCLVLAIIKFLLSHYGLTNRIEHFKNQIKISLIDLDSMTGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9Y9</accession>
    <name>A0A8S1K9Y9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0180416</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9Y9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8051949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="85" mass="10425" checksum="BAEEB9E4E5FC84A2" modified="2022-10-12" version="1">MRDILYKKDKDFQQIFMLLNQNFSGDGQYNDEVKIGRWIQLGEGLDYQNQIIYDAQYQNGKKLKIGQFLTKRKRKYQKDWWLKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAE5</accession>
    <name>A0A8S1KAE5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0170151</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAE5"/>
    <dbReference type="OMA" id="LLAFMMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052981">
        <property type="entry name" value="Ingression_C2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052">
        <property type="entry name" value="CONSERVED SERINE PROLINE-RICH PROTEIN (AFU_ORTHOLOGUE AFUA_2G01790)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052:SF3">
        <property type="entry name" value="INGRESSION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="103"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8050625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="381" mass="45214" checksum="7D8511C82C28F3B8" modified="2022-10-12" version="1">MNQQQYNEMFTAFQTNKILVEQFDIYLKQKCGHFYDEHLFQQLIEVSLNNGVIDLESFINQYNETVDTLQYRSEILNNKIKELKILHQQLSDQLNSDALQQNSLTLQIMDCQNIEIRDPSVYLQICCGIKTHKTRTMKGISPQFQEQFEFPIDSSCQDVIITLFDSMNDNMIGYTQFLISELREQKLKDISVNLQRQDNMKIKTKLHVILKFNHSKESLYKQEQQKLNHEINQNNLEIKETQQALNCLAKVFSGGCIKIRQLPPILEIKEFELKCLDTLNEALQERIQDWVPLFYKLIGLVVALSVMISLYRCQFMELTLLAFMMQLLILREINQKWMRVFTYLFAVLFLYDLLWLYICGGLWYNQRRTTHQQICYSIKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KBH8</accession>
    <name>A0A8S1KBH8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KBH8"/>
    <dbReference type="OMA" id="WDNTSFC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="108"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="347" mass="40804" checksum="169F66B7E758BB6D" modified="2022-10-12" version="1">MFTINQSLEKETYELLNDNTIYQNQWCGAIAIDENYNLLFVGHKFAIKVFELKNGALKQLNLLLNHSDYVTSLNLLQKKSYILSGSHDKQIVVMCTQLMSNPKYIKKLKGHKDTINCILPHPINEEMFISSSKDCKIKFWTFSSKHSRNHCYQTITEHKKSVMKLSINQQGNTLISLGMDKLILVLTQLEFFWVVKQKIYLNKLGPNICFITNEMFIFQPYQFFSEKHRSENIWIYAQDKKNQKYYSFQKDIQISDNTQNCIGSFPFFYNHQKRLLIFQNGNSLKIIKFQNLDQGVFNIEQIIIFSNKIQNNPLSFISGAISNDWKYLVTWDKTSFLLQVRKYVGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDL0</accession>
    <name>A0A8S1KDL0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0190367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDL0"/>
    <dbReference type="OMA" id="VFRIDNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="55576" checksum="C61199EDC8AF8003" modified="2022-10-12" version="1">MLTILEKFDIFGVPIKLKTIKQQEQFKTSQSGVITIIIMILSLAYFIYVMQQWINGQLIPKVTSSQRMIDYTTYEWQNGEISFTLYDLSSKINPFTKQNNIIMTVLLEISGLYVSAKPRFLYSTLQMNGDKNVITIDKGKLVLSQIDGTKEENQENIKQYFVFFTKCRQDWMEEGGYCADPQEIEDYFSTNHGLLFLSINLQQYNYETLEFDEIKKTQFFAFEESSPFYSQVILQKQLTTIDNGVLLNSFEKFEVIKDYQVNTQAVSKKFAQDTIKTQDGSKYEFESLCTFVFRIDNIEANETILMPKLGEVLAQIGSIVNIIMLLKILSNMINTTMLESTILHQIIEIYYPQFKQVQIKKNFLGQIKQVRYKGVEIKLAHFQHKYNQLLEIARMKFTFNNLIQELSRVQLVLVNQIGMENIKNIFKIEEDLKFFEQDRSSSNSNFQVQNLVSISPEESPKPQEFERFILFNTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KFF9</accession>
    <name>A0A8S1KFF9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KFF9"/>
    <dbReference type="OMA" id="HEFCKRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049942">
        <property type="entry name" value="DML1/Misato"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION REGULATOR MISATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391:SF0">
        <property type="entry name" value="PROTEIN MISATO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="361" mass="42543" checksum="0CD1C4C979E7FBF9" modified="2022-10-12" version="1">MNSLKQEKQEVPIINEQEEDEPQIIKLTQKYLHELKIDSKQIYNYKKNFENENDLFMTNLDSAQVEQFQDNIQTQLEKADQCDGFELVIDCETCYGEIGLKVVDSLNDLQGNSKTPKIVISILDPNMNTKNRIINRSLTLTQYQENSTLLVPLELKEMQIHSKQLELNQQYSFLINNILSSYKCTQEYVSMHEFCKRITPAPQNNIFNILASIPSLQDKPENNILNYKQAKNLDKFYFSQEIINMNSERESYGESYFLRGISPFPNERNLKSYGVDIRILDYIYQVPQLYDYTDVMPFHSSIFSNESYKYTLLKLYKELNSIKKMGFLQYEKQSFEDDQQLKYDLCQLIENYGGIDEEEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KFX0</accession>
    <name>A0A8S1KFX0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KFX0"/>
    <dbReference type="OMA" id="YKSICLK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="748" mass="91083" checksum="8A6CB1E35C3FAFBF" modified="2022-10-12" version="1">MLLFEYGHLKEKLNISGYFQFENLIRDFIRQQQNKQTILNQCLINKNQDNKIYIVSKIVLTDNYTTLNEQYDELLPKIQQFVDQDKGKELNVVLKSAFDIQSQINKYLEKFQQFNNQDLMTHIQKRFFMKQYLKQQTDQQDKYLGYFKDINEQLKKLKLYQQNQIIDLLIEADAETLEQVYNCIKIEKALQFQFLQEQQNIISDLELNNYFLASLYITDQIISDQNVLEEWLIQENKNQYILEIFNDTLKDLEGYQISFQDNSNQQDKRFIKFKEALLNQLKERFWIFSEEITQFNEDVQLKNEINNLFNRYYKSICLKLQFNNLLEKLIQIILDNDSMEGIVERILELKIQNINKYVLDLVDKYNLVDIDKKLSQLYQKKKHRYLKQLKNRPIFFQQVDIIIDYCNKTSTMIEMENSQALIDFTNSNQQQILEFHLMQTLYNYDNLYSDEQFLPELFKQTNKFCPDLSILKSTQYVIFQQKIQFMRLKQEKRIGKQQQKQKNKLQYLDYEKLDIKKVIQQHQNSEQSPAVIYENRIWNQFHNQIISECNIILDQEIKKKRNKAFDEQKKKYQSNYEQYSLIPFDWIETIFSKSYEEMKEEETMKNIDDIFKNCEKEEENYFKELQEYVEFENDIIKNRNKQIKEYFNSLGKKNYETIKELEEDLKNLIKLNREQLFKQQYLIKNSIQEINQEYIKQCQEILKYPPTHLQQYKVDKQTQLNDLRDINNQILSFYGEQLINHLKQQKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHY6</accession>
    <name>A0A8S1KHY6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0220208</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHY6"/>
    <dbReference type="OMA" id="EMFLINC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="370"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="477"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="766"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="769"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8054789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="888" mass="104352" checksum="05BA108858F0D69A" modified="2022-10-12" version="1">MNSLSQQFSLDQTDQNFCGPKVKLDDNRLNNVQKQSSHDLFKSSLFQISNDYIEVAQTPNPQFQSAKNNRWTRRMLTRKNKQEVSLKLVDNQVFKTLAKERLVKQFKRNLFLRSYVMSREYKDVIQNHFKYEQNSSKRVEFNSQQQDKQIFLIPGSKVTLILDLLSLLIKILCLWVSPLIVSFRPDNYIFMWFQLGIMFQIFIEIFIQTNRPINIQGETITNQKKILANYFTYHLFEDIVDISCWVIQYLNINNISNSVACGLIILVSFKKMLKNYSNCNETMFLKGSFNYALDIFTLIITILSFAHVMACILHYVGQLTVSTGESWLLKYSIDDASIWVQYNYSIYWAIMTMVTVGYGDITAANQYEMFLINCMMLLSSGMFAYSMNSIGMILKNNYDIQQRYKRCLIQINNYMKKGQLNQTIQNRVRNYVKHYIQTEFNENYGEVKEIISHLPAKLRQELTMGIQMNIMENISVLNHNFSQTILKQLSQKIEQVKYIPDEVIYNNGDLEDQYLYYLQEGQVLLCEERSNKVLQTIKVGETFGEHQFFTGFPAKTQAISYGHSILYRIHRNSLIKLFNSVSNKDFQRFHNIKDNIIYLNNYQVILKKCNICNLYNHTNIECPLISYRPDRFRVVMQPDKNIKQEMKRQKYKRAGDKINSLAMNSKVVESVQEFTSTQSMTNFHEIEDTNQQLNPKIYSGQIQQNTQGISSKLMIDFKQQSKDFFNFDKQGSDQLVEAPQRPPRISISGLQNLIKKRVVHNIMKKDLNKQQSKQSFNKTPSLRKQQSNVRQSSKHLTKDELEGQPSELDQNQFNQLEYIQSHPQQTNFSVDHYYNYQGYMQNDNLLQVIKKYDKNKMKGSRLWNPYKNSKNSDGAIFGSMEYSIVMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KI66</accession>
    <name>A0A8S1KI66_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cep192-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KI66"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054089">
        <property type="entry name" value="Cep192-like_D3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22067">
        <property type="entry name" value="Cep192_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cep192-like" evidence="1">
        <location>
            <begin position="196"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="287" mass="33156" checksum="903F651134E12C33" modified="2022-10-12" version="1">MHKQKLLIQPLSPSNKENRQEQFGLQKQQFLQKSNNDIYRQLKNEYSELKNIINVNKQSVNFGLVLPGNICEDDIQFQNISNETIIIGIQVVCNNTEFDDLDEYVYSARKLNGYDYKDRFMLACPAQKQFNMKVALKVPNIKDQKGLFGTIIITAINSNQQKIQGQINTNIQSQVLLPLIECSKRLMNNFYKVPIIQMALKLGKKLDCKIQFRNNSRIGLPLELEILNKNNCEIIVNPQSLSVQSNSQFLVTMQVKNQQVQLIKNVLIIKVKNSNVHFSYPFIIQVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLD8</accession>
    <name>A0A8S1KLD8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLD8"/>
    <dbReference type="OMA" id="EFHIFFV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="571"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="619" mass="72414" checksum="8D37DB79BCF7D3B4" modified="2022-10-12" version="1">MFQFIKDSFEQVASKVKQSGIPSNSVKVNLSQFEDQQSSFQTAIYQKYLRQKLINKLNQFVGKKVLVIDPFIADMLNLVVESNQILRENGVDQICYNDSDQLEVEANQIIFFVSPDRQYMKRIVRIIRNNQLANFNKKYLLIFCPRMNIVCKDYLEKEAVLGDLIITNFNFDLIPLANDLLSLEMNNCLKQLYIGQDMSILQTVAESIQRMELVHGKFKNIYAKGNYSKVVIDILKQKKQQGELIEDEISFKESKMHTLLIIDRKVDFITPMLTPFTYEALIDEVFSIKHNSINLNIIKNESALKDRPKTMKLNDSYYNRIKIMNIKHCQRILEEKRQNNKQMIQSMKQQDNKMNMSEMNQYLAKLRQLPKEQNIILDHLIISQHLSKQLESILFNMCVNQEVHAILQTKSSQVEQMIENLISFGAPITKVIRLFSLYNQIQNGMKTKLYDFYRREILHMYGIEHILTLENCEKLGLIGKKLNDTHIWEKIEKPLRLINEDVDHDAPEDISYVFLAYAPIIVRIFEELIIKNTWGPVVLQALKELPGELVMQQNLNQLKGEEIKEEQNQIYVIYIVGGITYGEIAAIRWLGLRYKKDIQICTTHIINGDRLVQEMIQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSH3</accession>
    <name>A0A8S1KSH3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240344</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0240345</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSH3"/>
    <dbReference type="OMA" id="RFTHIQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8056505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="290" mass="34024" checksum="5A616E36C60C691A" modified="2022-10-12" version="1">MDTQFFTSSTSMSSSSSSSSRKGSQECFSDFGIEKKSRKSKKSANRSSSFALGSVTEVSEDIEDYLMSRLPHFQMVNDNNEFRERIPPRLKRNEKYQKRRASLSPHGTNGLAKVEEVIGEDDELKNKCIFKLGLMLDKDEFNDKRRRLLQSKDRINIEKATTTNLTILEDAQENVKELQQHVMSPTYKLKVDEVDFEQEKKQWQPQEIIEVIEEEKPDNIVNIKEKEMFQKIKKILHEERDFLKRILKGELNKQEDIQKKQQFQSEVGETNWNQLIQEAEEQVNSDEDYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KT83</accession>
    <name>A0A8S1KT83_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thymidylate kinase</fullName>
            <ecNumber evidence="3">2.7.4.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0210329</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KT83"/>
    <dbReference type="OMA" id="YWHQFDA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000679">
        <property type="entry name" value="Thymidylate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF1">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="10">
        <location>
            <begin position="20"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8054164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="216" mass="24539" checksum="C093BE98541548F4" modified="2022-10-12" version="1">MEIIIQQTIKKMNRGLFIALEGLDKSGKSTQAKLLSQKLNAQKVSFPDRTTQLGVIISDYLRGNKNMSDEVIHLLFSANRWEQHTSICNLLQNGTNIVSDRYAFSGVAFSAAKGLPIEWCKAPDAGLIQPDITFYLTAPIEELCKRGDYGREIYENSSFQHRVGSFFDILALQENFYKIDALKSIDEIQAEILNHISEKLKNNSLLQQKKLWKQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWG1</accession>
    <name>A0A8S1KWG1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0290060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="IEKGVHH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="377"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="481"/>
            <end position="1803"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1843"/>
            <end position="1870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1952" mass="232196" checksum="5291646836C8DEE8" modified="2022-10-12" version="1">MNNVCHILKNQKLQFIYLQNVNENQNDQNEKLFDQIQRIEDELSKGRLKYTQIVNELEKKDTQLKEYITSIQNNEQINQQIRKKNQELEELINRLKNEIQQKDTQIGKLISKIKDQDDKLQLKDLEIKNYMKENEKQQYASIEQQQTQHDSIKKEKKRTINYMTRNKSNTVLLKSETLHSRSQSHYIGQFDLEQFKEQCAASVKSQNSESEDDQTDKKTLKQENDEQQELERLIVELKSNLEAKTNQLQIVENEKKQCEAQIITLMSEISVFKIKEKKMTLQQKQQEKRLTELQSEKNKFQLEQEQLEQRMNEIQGQHQQKMKLMSSVINENDQLKKRLSLNLGRVETQQQIDKDIIKLKQEYDDMIMEMQKQNMDLHFVNEELIEENNKINEQIKQISEQQLNDIVKIQQLEKQLEQMEVELITQKQINLMDDKNIFEDKISIIKQELLINYDIINQLEKNKQKDSQNDTIMENQQNYLKIELQEQIKELELANNQLKQQIQQYQIFKQQEEKIIQNQMELQSNDNYLKNEQIETQQAIIQELEQKNIGLQEELKEKEALKLKEIEIIKLEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKQKDLQKQQEVELIKVEIEKGVHHLLEKNQQIETQQAIIQELEQKNNGLQEELKEKEALKLKEIEIIKLEIEKGVHHLLEKNQQIETQQAIIQELELKTIQLKQEFEQANFQNLHQIEQLKIEEQQKEKILQQQHQSIQSLEKLIEQQNTQLEQLKNNTFIQNQEQQIQILQQEIHALKLQIEQQDDHQKVYESILNKQIEKLKQINLSQQTQIHSLTQDKEKLQSICSQLKQIENILQDELHKMQDKIDELCIELDKVQQPSTRSSSISSQNKTLIYEQFFHLTSQSVQMNSNHQNAFDVKLKSYYSEVVNQGVPFHKWSKWLEDKLKEELNKFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYJ5</accession>
    <name>A0A8S1KYJ5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Beta'-coat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0260178</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYJ5"/>
    <dbReference type="OMA" id="FWICAVH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22948">
        <property type="entry name" value="Coatomer_WDAD_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000016">
        <property type="entry name" value="Coatomer alpha subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000002">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047312">
        <property type="entry name" value="Coatomer_alpha_WD-assoc_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010714">
        <property type="entry name" value="Coatomer_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF1">
        <property type="entry name" value="COATOMER SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06957">
        <property type="entry name" value="COPI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5 13" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="47"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="131"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="191"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="235"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="15">
        <location>
            <begin position="632"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer alpha subunit C-terminal" evidence="14">
        <location>
            <begin position="868"/>
            <end position="1189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8057706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1190" mass="138805" checksum="6E9E488E3409F309" modified="2022-10-12" version="1">MIVKFHKKTERIKGLSFHPKQPWLLVGLHSGEIQMIDYRFGRTINEFYEHEGPVRSVQFHQSLCLFVSGSDDFTVRVWNYKTKKCQFVLRGHLDFVRCVNFHSELPWCVSGSDDQTSRIWNYQSRQMIATVTGHSHYVMHCEFHPSKDFMITCSLDQTIRLWSIAQLKKKFTSKSIQLGEQTNELELVQILEGHSQGVNWCSFNPKDNTILSASDDKKVKVWKYYDTRGYEVDQYNGHTNNVSCVMFHPFGEYFISNSEDKTLRLWDMKRKMEVDCFTNYELDRFWICAVHQSNNYFAGGSDSALYIFTLFKNRPAVDLVNNNIVYFGNRKLIKILDLQNGSDKTIKNLQELSCISDNLLQDNVEQVLYNIYENVKPQIIVKLKHHSHSKSKGLSKFLVFEQLTNVSQFFLAKSAIFIGKSKILRSKENSEIEIYNFETDNHIPLGHKTDRLFTFIGGKAIYYSDQMINVLDPISNKILNQFPCTNEFQNIKQIKMNDYCILIQTKNGIYIFTKEFHPITRIQEKINIKSVLFFNDSINIILYSTKVHVKYLLLNGDTGIICSMETVPYLVSFQNIPEKPGFQYKLYYMDNMDKLLNITIECSEIFFKYALIEKNLQFVQNFIKNHQKLGDLIISYLYQKGYSILAHQLVNDKRAKFQLALSSNNLEIAYRTCEDLKNPKCYQMLLEEAMRQGNHNIYEVCQQKLRASGELAFLYTITGQVEKLNIISKIAQEQNNQDLRFQTLLNMDNLKQRIQFIKSCNLDKLANLSKLAHGLEFEKNKNCSEDIEWLQSLKPQTLITPLPIIRSSQHTLFSINWPHNFVDEDELFKLSGDNFKIGNQSKIDIINTITTNHQDLFDKNIQTKQKVNIDKTEENDEQFQDCQWDLDEKELLENIEQNQAILDQKSLLYGYPDYKKALQPVEYLITEQYQQCHQVLQQNYNFVSLKVCQDYLKQLALRSILEVPQIPFLMPSPLIVSSSQDKIYINKSFSELLKIGYKQTSDGKFQDSLNTFKQILKQAIFQNLNNDVVPICLNYIMAMKCELAKKDKDISRQIELSCYMAMCDLQPAHRSLTLRAAMSLSYKNKNYLTGIQVAQQLIKLLEQAPKGATYAKPEVLDNVRKIYNNCQQNPKNEHQINFEPFYLNEGTKNLCADTITYNNDVKQIYFCSFDKSRHSKKGILCQICELCNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYY7</accession>
    <name>A0A8S1KYY7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0270329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYY7"/>
    <dbReference type="OMA" id="VEVWKTR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="317" mass="37076" checksum="937D6D6B0A3EA638" modified="2022-10-12" version="1">MINTMTKREESFLTTKVSDRVITVENEQRSTYNFVRRNYLTTTFATLANRQTNGVYNVAFNTSCCQPCPQPCPQPQAMPMPIYIPMPYPQQCEKECHCEEEEKYKEEVLILRKRVAELLSRQPQVKVEKETVKVENTTRIADLQMDIERLKINLRNEQDRLRQKEGEYLVLRSDNSSQSLKDRLAIFESQLYNSKLELEKLQGDLRQKLEELNEWEQRYQHMESTVTVESTETQTLTNEVEVWRTRFKKLNNDFFETQEKLIMAQAELEALKKGGVTEVKSVTVQQNVTSSSVNRVIEQSSRGSRVIDQNLVGKLYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZK6</accession>
    <name>A0A8S1KZK6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0300114</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZK6"/>
    <dbReference type="OMA" id="SEYCEGA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14014">
        <property type="entry name" value="STKc_PknB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000771">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="85"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8060397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="629" mass="73995" checksum="BA8B02E367846AEC" modified="2022-10-12" version="1">MQQLFQHIIPIYILQVKLVIKYIYSLEQQDIIFFQIYIYIYELLNSCVDYTQKQFIQKNLQISGKLIEMNMHKEVIRKQIGDYCFDMNDIIGEGYSSKVYKGIHMVTNQVVAIKVISLETYNSPIQKSLLSNEIKILLQIDNPNLLRVYEISQSANNTYIVSEYCEGANLEELLKQEPLKIEKVIDILTQLCKGLLDLHKKNIIHRDIKPANVLIKNGIFKLVDFGFAFIENQYDSIIKRYHVGTTMYMAPEIHLFNQYSEKTDVWALGIILHEMLFKAAPKFKFNDQTLLQEIKENCQTLEIIHQNLIFRLLDGMLQLDSEDRLTIQQILNIIQENKSSTSKQMSHLVKLSQKSISTQINSPLQSKSVKTQNQIRKKYVQQLQSNNKPQSFDEHNLKNIVRVQTQNRDQLFEINQFISEDEISGKFIKTNLNQQPFRYSHQKSDSDINIELIGQISTDCSVQKSPKQKLFIQKKDEEIDLPVILKPTYKFCEFVEQIIQNFPQQDNDLILKMQFLLRKLLAIKANVFYTFAPIKIKSQLDNWINQLHQYYQKIQQQINLNLDKTFQLFFNSNLDDQGKLLTMYIISLANQMFKKSNEINIVVEILEDNLQHQNDPFLFARKWLFQISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZV9</accession>
    <name>A0A8S1KZV9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0300195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZV9"/>
    <dbReference type="OMA" id="YQPESIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="433" mass="51785" checksum="6F0CF2A2CAB897CB" modified="2022-10-12" version="1">MIFAELLKELDKQLLDLLIETFVYIFEFYKFHQQMNFIKSIFSKPSLTIQQQKPKNQNSDFSILDPQFTQVRLRDAKLLIKDMKKLEQVVNYQPESINEQLKKCYALHIILSSDQYQAIDKVVMCCEKNHIKPVVQNTQAKTAVTTNINQTCQIMSDQQMIATQFQNSMYLNVSDQLQHYEQIHQNESEFMNIYFNYIQRVTQNSDVYNSCKFHQYPYQDETFNRKLQFVWLFKIINMLNFKFGFLPYLQFSFNHKTQNSFIIRDIAYLIYKDCLLEYGFLRNEITDMIESYSQFQVRDSLQFYELIVIMLEINKKMKIFYDMRQQFALNSSSVRDVKWFVIEKKQLIEIENFISKIKLLNTAQFKKSLMVPTQKNVMENLQKVMMDPSLMENSFLKEPTTAKNIKKINELLYSPLNSKQIKISNHSRQLSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2L6</accession>
    <name>A0A8S1L2L6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cystathionine beta-synthase</fullName>
            <ecNumber evidence="4">4.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0300123</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-homocysteine + L-serine = L,L-cystathionine + H2O</text>
            <dbReference type="Rhea" id="RHEA:10112"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="4.2.1.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.22" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2L6"/>
    <dbReference type="OMA" id="TEVDCWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004122">
        <property type="term" value="F:cystathionine beta-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006534">
        <property type="term" value="P:cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009069">
        <property type="term" value="P:serine family amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000003">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000118">
        <property type="entry name" value="Related to CYS4-cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="7">
        <location>
            <begin position="44"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8060415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="500" mass="55966" checksum="59A4D2E9A2D720CA" modified="2022-10-12" version="1">MIQDTSKDTIQCTYKRGLVEQQAPTPHSNFKFVPFKKIYNDALDAIGHTPMIRLNKIPKEYGLKCEVLVKCEFVNVAGSIKDRIGVRMIVDAEKQGRLGPGKSIVEATSGNTGVGLALACAVKGYPLYITMPEKMSQEKQDVLTGLGAKVIRTPTEAAWYEPESLIQVAKRMAAENPDIILLDQYSNPSNPLAHYEGTAEEILWACDDKLDAIVMSTGTGGTVTGVGRKIHERVPGCQVIAVDPYGSDLAMPPEINKTDIKSYKVEGIGYDFIPKALDRTEVDCWIKSNDKDSLTMARKLMAEEGLLCGGSSGANVWGALEWAKSQNFTEQQRIVIILPDNIRNYMTKHLNKTWMIENRLISYDELKESDHPLAGKPLSELNLPEIPLLNVETTTIGECLDYLRTYPAVPLQKDGKLLSVVFQKKLLIGIIDKKLNNVIIYYNKIQRDLAKKVQSKDFVVVPNRLDQNQLERIVERHEVVFIEDGEKLRYATPLELLQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L6M4</accession>
    <name>A0A8S1L6M4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0310171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L6M4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="55" mass="6342" checksum="7F0AD2313AF640F2" modified="2022-10-12" version="1">MNEPCQLCFKITIINLMMPFGIMNSLMSLLEINNNLSLKVRFNQSEANQVLCHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LA14</accession>
    <name>A0A8S1LA14_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0360241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LA14"/>
    <dbReference type="OMA" id="REYICEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="149"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="274"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="452" mass="54343" checksum="8D986948F5E49222" modified="2022-10-12" version="1">MNYHNPDPYQKPYPHGQYNEKQYRYSGFLTETQDDYTDYQYYDPYQSQLHNRTYLNDVQPLNYTYNEYPPYPVKMGQPQLYFDPNDPSNIPFMPHERYDDGYQEYVLQFIKNLPRSTQNFEEVAFAKYIAECSIIDKKVWKLQRMLKQDQRFNVKLLFDFFDSKKNRTIDYNEFQLGLQKLDIILNSIELTQMFLRYGGNDDNQISSAEFKYMLLGPDDQQQFMQFGAMHYMHHPLKQNIPQFLSVAQREWICEIMNLQLQLERNLNFIREVIGTKDILLALFKQLDIGMKGYITLQDLVVYMNELGGQFEQYDLKGCLDRMSKVHCSRINYNEFKKEFFLDQKQKSDEDQNIDIMSDRKKTRQKDYIQELHQRTKDIQNQQQQIQKLSTDRKSQPLLTTTIQNKQPQPYVIQPPVVQKKKQEQPSQLDDDLDDLLFPGFQKYELQPPDQEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LAF7</accession>
    <name>A0A8S1LAF7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0360151</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LAF7"/>
    <dbReference type="OMA" id="SICEYAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000093">
        <property type="entry name" value="NLI interacting factor-like phosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="54"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8064487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="329" mass="38590" checksum="E1A324171842225C" modified="2022-10-12" version="1">MRANNIIVQEEPKQNVLVAFYNTLKSLIKCECFKQFMPISYNQKPIQIPKQSLQHLGRKTLVIDLDETLVHSSFNYISDPDFILKIKVMNANYTIYVRIRPGAEEFLIKMAEFYEIFIFTASICEYANPVIDRIDQKGVCALRLFRPNCSILNGVFVKDLSKLQRSINNIIIIDNTHTSFSLQPKNAIHIKNYFDDPSDTELLDLIPFLQLLSTFDDVRPVGEYWKQYIRSEKIRYTRNGESYIYDRNRNESYDEVDPINQTIDQVKDDQMLLGKQQLKLQTNFAIDYDEVNDITENDEEIQFRNEQGINSPNLNKQHFNEIDQSDQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFQ5</accession>
    <name>A0A8S1LFQ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0360093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFQ5"/>
    <dbReference type="OMA" id="DQKQIKP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="307" mass="35948" checksum="4954AAD78195DBE9" modified="2022-10-12" version="1">MYSKQATISKSQNLKSKLSIKDTMSNSHQIHSQPQSSKGQDNLKQENKQDCQLMKKKLFNILGKNSESHKIFKVSPTNSLKQQYFVSASLQQKSKGSMLKYNHTTDNNDSLKTLKCNALKLLKSNKYKDINKYNEFLSNNNFEMQKQFGNFVRSIDEIQAEQLPIQNTERSTLIALFEKLNEKDQKQIKPKRQTLQHSESYSQKQSIENEFIDLRLKQRNKCNFSNNSSGQLVNPNSTTTQIHQNPIISIEQKIKIDENLQTIVDKTKQILLKYQEKLKQNDIEKDVLIQEIQYWKSKYLKCKQQQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LG25</accession>
    <name>A0A8S1LG25_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0360164</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LG25"/>
    <dbReference type="OMA" id="HQPDKMV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12241">
        <property type="entry name" value="RRM_SF3B14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000432">
        <property type="entry name" value="Splicing factor 3B subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051847">
        <property type="entry name" value="RNA_proc/Spliceosome_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034150">
        <property type="entry name" value="SF3B6_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880:SF1">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="14"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8064513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="117" mass="13968" checksum="817685A5EDD4335C" modified="2022-10-12" version="1">MSRQVIRLPAEVNRILYVRNLPYKITSEEMYDIFGKFGAIRQIRKGTHGDKKGTAFVVYEDIYDAKNAFDNLSGFNVAGRYLIVLYYQPQKMQERQDLEFQKKQIEKLREQQRQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHK6</accession>
    <name>A0A8S1LHK6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0400215</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHK6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001267">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="9"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8067307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="539" mass="63675" checksum="1EB7C365ECC346D2" modified="2022-10-12" version="1">MSSKVVENYSLLEVIGSGQYGKVYKAVNIKNNSLVAVKVVKIEKFKEVSKLEEFTMNEIQTLARINNPYVVKFIEMLKSSRNYYFVYEYCNGQTLEAVIQEQGVQTEKEALYYFRQLVQAFQSLIQDNIMHRDLKPSNIMLHNGQVKLGDFGFCKALNGPQDLSSTMVGSPIYMAPEILKGQEYSIKADIWSLGCVLYELLYGICPFEEKTMAQLMLAVEEREIQFLDNVNVVSQTTKDLLLKMLTKDPNKRINWKELFERELTYAERTKNNEQYTNQIQQELTLNSQRNKESKAFKYLLMERNKIFYLYKVVEEVLELSKEERDETRESQSIYCAYLIVKYICYLIEMIRMNLVDKFNVSAFPHLLKKQELQNDITKTFEYKSFCTLIQKEVETARKMFKHFQDEADKFFKSQNNGQHIYQGAEFSHFESEIQRELQSSEISINFMKKIVLKYCEDLKSQFLQQFLDKNPQLGNKQLLHIEKTLESLILDEFFENCMDIQQIDLEQQNYFTILNKYSKGDLLQVITNKMDYIRHKLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHS5</accession>
    <name>A0A8S1LHS5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0410114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHS5"/>
    <dbReference type="OMA" id="YYRGQWK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="37457" checksum="5872F20DFC6713D6" modified="2022-10-12" version="1">MGSCQICNGLQIQEIKMQLKPEIQKIEPTFKQVALIQARFRGIITRKRFQIHKQKENINQQQQNSLNEDLICYDYAQQIITERSTVAQYQDDHAAILMSPKIKNCKFQFGSMTGFGRMVMEDGCYLEGMWLDGFLNGEGLYSVPNGTYYRGQWKNSYMNGQGIYFNSTQNIRYEGEWENNLQHGQGIETYADKSVYKGIFKNGLKDGQGQFIFNDGSYYEGQFSQDKFQGLGKFVWDGGKKIYYGEWFNGKKHGFGQLEVKGKYSYEGHFKNGLKNGIGIIIWNDGRKYDGQWKDGKQHGIGTFQNKNGEILNDVWKFGLIQHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJK5</accession>
    <name>A0A8S1LJK5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0360351</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJK5"/>
    <dbReference type="OMA" id="SMTNHKA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="329"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="627"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="998"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="834"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="58"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="998"/>
            <end position="1011"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8064886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1073" mass="124737" checksum="C41500AFF707B8C3" modified="2022-10-12" version="1">MLVRSSLSPRRKCFTPSMWEGRQQQTIRRSQNRYLKTEDVDHRNVQSRNDDSKRKSGSIVNNKKNKSMTNHKASLNSSFNTKNLKTIQLSTRKSSLQNKLHPNSTTSEHHVHQYFSQSPAKRILNCEKLIKAQKFSEALKDLEEIDQPHINPEYIKEIAYFKGLIHMNCRQYDQAINQFSRAKQSGDLNGNTVILQALCLKKIGNYSDAIVVLKEFLSKKYSKLQPTYYDALIHKGKLLMKIKKYQLALQDFNQASVLENDQISFQGTLGRADCLRLSGKIAEALNVYETIPENEIILMRKIYCYIELQSLDRAMEAINLILINDPNSSEALFMKGQIYINKGQLNEAILSFEQSIKQNNSRKAVTKSLQEIAKIKIDQKDFYSAYYTLQREDHLEVDKESIMKLRQFTEGVIFLMKRKYQDGVNVFTQLINNYQLGEFIKKIIFLYRAYGFICLNKFQKALNDLIYVQNFQELDQPSLYNKIICEGIVMSQSSQFEKAQSQFQKASKLFPGKMEPHFYKSLTLIQFINKHMPKDKDKYIKNALKHLDKAVTLNDQNSNLLYHRGILRFYFGQIDLALSDLTKAVEKNEDRVAKYVYARGLVRACLDNPQQALNDFTIVINWDSKFAEAYLNRAKVFTLLGDRTAAFNDLQTYISLKPKDPDIHLWAGNLLFLIGAYEHAIKTYSHSPDIQNNVQLLRYRALCYIIQKELNYAMSDLNRIIELTGDKKSYIDKECLLALKTSTVGPEESQSMMILKLSQGSNKQVNFRLASQMLKKLIVLGSDGHVFLTSDLIFYRGIMKFYLGNYKKAMDLWTKSYGLKQQIKEFSQQSNNSLISQQEQQERLISELDELEFEDRTYNMYEYYYNVAIATILNKQKRKGKELLNQLADQLQESFETTIRDFLAALDSTQPKSATIFPYSNRLCCIFPTFKIGNLETRLSFCLPRITPPSMNPEFDNKLIEGIQSTDVDNKPEAPWIRRNPDGVIFTENVQQVELDLRSESQKSQIDHNQEEENDDENSEQHQISINDDKITIEQRHQDDSTTYQTNYKKDIELLKQQLRLDRIVEQRLAKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJP2</accession>
    <name>A0A8S1LJP2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0390203</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJP2"/>
    <dbReference type="OMA" id="CISIQIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="464"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="498"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="566"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="188"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="56"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8066615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="673" mass="78897" checksum="7D92CB2CEB0351E7" modified="2022-10-12" version="1">MDEPEFLCHYDGHDEEEIIGFCINKNCQQNVQFCLKCSWDKHGDHEEDCKTFKQIYKILQNNKQQQTGQNDEVNNKLKSIQIKCEQLSQSKNIGNEKFDELEKNLKEKEYQNCLANIQLLKNIQDQNQSNQKLILEQLDNILQTLNTLGIPQISNTLKQQSQNTDNIQKVEMKNQEIQVNIEEEKKEEIQTLNNQEDEITYQKQNNESQKPKDKYTSISISNQEQSQKFEDQKYPLQTPQDPISNAYQQTLQHFYTENTPSIINPYQKSNPSSKQPPIFQVPQNPITQSMAQFSEQNLEQRQIQNKSNGSQSEIIKQKFEQQLQTAINKNPEYKNNPHPLIKLTDLEWEQYKNVIISAQDQNQNFVLDAEVINYYDDEYKKVLEECDKSLEKDPFNIQQNILKCKTLIKTNRNQEALECSELIMDMDPLSYQPYILKGVALNQLKRYEEALHNYCISLQIEPNFEAYLLQGISLLAINQWDDAIVSFENSIKMRPQMEDGYYHMGLALMHKQKYEQAIKYFEITLRISPKHSQAQKQKTLCLNKVNQYSEAIHSSDATNQKAQSEEYTYYNKGLELFNAEKYREALQQFEKAIQINQNMHEAHAYRGETLHNLGRLLEAIKSFDDALTISAKPQYMIKKAETLKEMNNFEAAQLLQQKAQLIMQTEKQNPQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKE5</accession>
    <name>A0A8S1LKE5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0380096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LKE5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="70" mass="8366" checksum="94C247570B25A716" modified="2022-10-12" version="1">MNQGNSSQYQVNDYYNPLEGQLNYKNYYRIKVLFFYNFSYIGNFAILVTLGIILTHLFTQHRKDTVLTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LL04</accession>
    <name>A0A8S1LL04_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inositol 1,4,5-trisphosphate/ryanodine receptor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0400139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LL04"/>
    <dbReference type="OMA" id="AGENKWT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF4">
        <property type="entry name" value="RYR_IP3R HOMOLOGY ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol 1,4,5-trisphosphate/ryanodine receptor" evidence="1">
        <location>
            <begin position="44"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1220" mass="143983" checksum="A86F8D3F2710473F" modified="2022-10-12" version="1">MIDLPPIPTLKIEQQVAGENKWTQTQNCNSLQQNVDNIKHQKQNLNLKVGDIIYFKSYLSDDFKGVISGDGIASNKLECVYILGKNKLQDIQSQKSLQFKVGSLSFQKCLFQIITGKKYLYQNQYKIQKEKPVDQRIITEEQTQPSDNDQKLEEFKKKCDDEIIENGKFYEYQKGSNIVYGQEIQLLHIYSGCTLTLKSGILAKENCCRELSLEENSNQYSNFRILSMNSVKNIGEPILYGDQIIVQNCIQSQWNLSVQMSSDNVFKKDGLEVNASEQAYPLKISSYIDNKTEEDINKQQIKGKYLQSGDVLTIKNRYLGGYLCIKRIRRITDELDKKELIINYSETNIQYSINQYYFDVDKIRNEKINQMYQLYVDTTQDADENLNCLWQIQHVDSLIYQKSTYESVFLIRHVCTGLFLQITNNEVGLTYDGLKMECQFNLKSKKQSIDPISYSETFKIQSAITISIDSHQTQEGVVLVCQDDKKVAVQRKSQKQKIEKATFLLKPTSQELQKTSLRINSLQEYLIQFYLFLQDWGIVKSIDKNQQEKRKYEYYEAFNNQKLLFDEILQLYQTLQNLNLYLKNEGQPQSSEQQQCKQNTLMDNDIINLLFAIQGLCNCMIYGNNNILFDKSPQIIAKMKLESQKKNCITEIYSFLSLCVQSNPKTSNYVLSLQMYNESILVFLLQQLEHQRDGVSNLIKETVRYTDKTDIKTSLEKWVNQLQPITEKNIEDQALYIEIISLMMIDPYENPKSFSQNSCRKLLFGKKHHSDQSFPFSKALIALDIQEENNNYYPYVLFSPKREKGTLLQLSNQFGENNPIFCQLYLRFVEKQIKNSNRFKKQRPPLVDVTIDFFTTETLKENQLVRQEEYKKVVPIFQKYENYLMNVMDLYSSLCKGRNQKSIKCLMKNCFLNQNFFEICLISQKQIKTGLRFERTLIELFCNLYLDIDPLIKISQFDNKCYLNDDLDQFDIKNQSGIYFYEKNASKQLKRNEEYKKFLNSNRAQAKHQYLKLFAQKMLSRKQINDVRSYYLQIFAQIPYPDHFRVLEFNNNIFQPSKSNIEQINIDYKRLANYQLKYFLGILQIIKNSIDLGYNNLQENYRIFSILPNMFVALILQQRQDNKIYKFKDNESFESNLLNLKKSIENNLWVNTYLQPTQEQSENIISQKWFHQVIKQDIQFQRNWILQFLIWTFQYCDDELLKMKIYLEGLQIQKFLVSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPK0</accession>
    <name>A0A8S1LPK0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0390092</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPK0"/>
    <dbReference type="OMA" id="GGPQCMC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000961">
        <property type="entry name" value="WD repeat domain phosphoinositide-interacting protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8066446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="345" mass="38870" checksum="6C34E24691AF055D" modified="2022-10-12" version="1">MNSTKSEILFAGFNQDSTMFCVGTDTGFRVCNALNSTEKFQRDLKGGIGHVEMLYRSNILALVGGGLQPKYPDNKVIIWDDHLVKCIGEMSFRTKIKNVKLKNDRVVVVLEKKIFVYNFTDLKLLDQIETCPNPRGICTINTEGDHTILATLEKSVGKVFVNNYDANKAYCIEAHVSPISYLQLNSTGTKLATSSEKGTVIRIYDTNTGQISQELRRGNDYATITSLAFDFRSQWLGCASDQGTIHIFAVNQEGLQQEQQIQNQVSHNPKSKFEFLKGIIPILGSEWSFAQFRVLDTKCKVSFVPDEHQLIVISYEGKYYKAQFDPQKGGECIKVEEKYLISDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LST2</accession>
    <name>A0A8S1LST2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LST2"/>
    <dbReference type="OMA" id="KVRFCKT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="130" mass="15453" checksum="E60F7195A5014037" modified="2022-10-12" version="1">MGCAPSAIQYEKVEFLKDDLILSDDLELSDETCANLINDSIRVNESILNRKTLYKEEIEDFQIMQEQQLNQKPFIIKSCFKRNNKTQQIMQKEKKVRFCKSYQININGLKFIVKKKEAKKKQKRIYNKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUM1</accession>
    <name>A0A8S1LUM1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0460215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUM1"/>
    <dbReference type="OMA" id="CETQINC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="586" mass="68948" checksum="1D5CCF33607B1560" modified="2022-10-12" version="1">MKSPEPLKCPEHTKPLSKICIDEKCDLQPYLCVDCLQNHLCHSYEIQNGIEEFAKKLNVNLSQFDKMQIQFKQLDTDSFASFIKIQKQRVSEEMQEIKKKIMQKCDNLESKLLETLEEQVIIYNKNIQQFKQQLKETEKFQSLQKSSDLSILNCILGVKLPNHNDPINIMRKFKSHLLNYTTFFSSGKIKQLQEQLILLQSQTVFPPTFKNLEFQKNEQNRLINYFDQYLSNIESNIKTYIKSFPITDFDQKSVQIKSIKKEQKLIEEDQCITSFLILQNNLIYALNDMTIKIRDKCYNEVVVIQCETQINCIIEINCQITELAQQSLINKSQKLDFNKQIILALGGDDFTLIWQCFQSSIQQIRKVQHQRMSRVVSLLSFDNLLLIGDWDGQIFGYDLNIQNPLYIVDTSSTILFALVKSSKFIISASKCDLEQGGIIFWNYNKNDLYQEKRIKIQSVQKMAQSNDQLFVVDIKNKLYIYDCITFELIRSFICSESFVNEIAIDNSQQILYVLNQTCLKSISDQFQWNYQSYEDFPCLQCRQYYQSTRLFVNQNMIAFIRHLHQCKHEQGDEDKCSMKSTLYLMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LWV5</accession>
    <name>A0A8S1LWV5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0450030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LWV5"/>
    <dbReference type="OMA" id="IQLHQGY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052920">
        <property type="entry name" value="DNA-binding_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358">
        <property type="entry name" value="ALPHA/BETA-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358:SF4">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="39778" checksum="0F6C9BE366368FE4" modified="2022-10-12" version="1">MSIQREWQKFVRPPRYNYSYFDLGPEYIQLHQGYLRKHEKIITNRQNCKLQMSYFELNPKTSNCIVYCHGYNGCRIEGIKYASIVAQFGFNFCTFDFQACGHSEGDFITFGHLEKDDITSILHELEIQFQQKQFILWGRSLGATTIQLKEQPNVKGLVLDSCFTNFNKLAISIIQKQTRLPKFIIKAIIYLTKGSIEEEAGFQFNDIQVQCNSNMPTLYICSDKDSLIKAKNSLKLYKQHKGIKKLIKVDGEHNDSRPLEIIQQICSWCKDRVQIQQNNYIQYNSVPQVARLPLELIYDSNIQSGMQSSYNRIPKNPAIKLKNQISIQLRTSCRNLHSAVQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LX92</accession>
    <name>A0A8S1LX92_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LX92"/>
    <dbReference type="OMA" id="DNHLFKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="225" mass="26575" checksum="D11BA33B1E35ABF3" modified="2022-10-12" version="1">MIKHSLTQTSSTNHNFSQKLQLQKLKRNKQMVSKAIDMLQNPFDPHQVSQSLRFLENQSQRKALLDEIIGIREERKRKQEFEKMSSMRGILTKVQKRFSVVKTQDIKFNRDFNDFKILQEERNKMEKLQYENLNLQINERKSQFKRMSSLNFLTRESDNHLFKHQLSLDPGAPQSSLMLTRKTQRNTTLLLSKIANKQIHTEGNTEKVLTSPQNCLIITQETDQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LY65</accession>
    <name>A0A8S1LY65_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0510206</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LY65"/>
    <dbReference type="OMA" id="QEKRCID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045234">
        <property type="entry name" value="Unkempt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057444">
        <property type="entry name" value="Znf-CCCH_AtC3H23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493:SF50">
        <property type="entry name" value="RING FINGER PROTEIN UNKEMPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493">
        <property type="entry name" value="UNKEMPT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25512">
        <property type="entry name" value="zf-CCCH_AtC3H23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="121"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="157"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="121"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="157"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8073718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="451" mass="53475" checksum="F76C632B45BB275C" modified="2022-10-12" version="1">MHNPQDQLRKALKRYNNNGDKPNNNRNCRSQQLQMPQVPKGMLSMNSAQKSERNAKLSDTDTTDVTHYNLNFSQPIQSSYQVEHLDLSFFKIQPCKIPGNHSHKHCPFFHNAKDRKRNNTQYSAELCAYVESNQQCPYGDNCNKAHNRVEQLYRADNYKTKFCSYYPNNISQCDYGKFCSFAHSEADIVIELIHNLEYDDDFFMFYYKTVWCPFNLTQHDKALCVYAHNWQDFRRKPQIYQYHPIPCPSWNTAEYILEYYNGCQEGFNCGKCHGWKELEYHPMLFRTKQCINQNCSKIDCSFYHNNQEKRCIDQLSQFRVFKIVPRNRIVQNIFKVRDKSLLNSQRNVSNKSQDLTLSYQRHCSTSDQQWLGHNLQNSFQYEQDSDEGKQNSKGQHYQTTLISIQERTDSDELKDLKDLMRKKSNSVVDDKQNNDDNEHVRTVLKMIDMDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0J5</accession>
    <name>A0A8S1M0J5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0500176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0J5"/>
    <dbReference type="OMA" id="LWNQYSD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="389" mass="45208" checksum="BBAEDE5263A17684" modified="2022-10-12" version="1">MELKYVNKFVEEYKLIKNDKLIKYLIILGIGFVKQRLEDINQEAIKLLANSFKEKSIHQELKNLRNKVLSLEKHIQPEQHIKYHSQPKLMPPKVTPKITEQIPLQQNVKKAVPFKHHLDDSEIKSILWNQYSENSHHISNNDSQNQPPRKQTIDQQSIIPKYLPRNNSQTEIHPLSQRENSTRQVTQRSSHHSSQFDSLHNRNSPKFKGEKKKSICKENKISGSIGGLPKHLRYVQSKIKSQIQYDKEQYKSNNSQKEEEQTINIANSSLNPFNNTPQNKLFQPCHSTHFGSSQVSSAKIQQQQQQQMQIQDLNNITERKVFNLDEIASSFLNSPFMKSQITQRLFAKQESASQSSSSSTFSLFNPNNELKNFFQQLDKQVGNSLSFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M429</accession>
    <name>A0A8S1M429_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0470066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M429"/>
    <dbReference type="OMA" id="PRNHSKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="89" mass="10548" checksum="D026233AB337F7FA" modified="2022-10-12" version="1">MSSYLPFHNLAYCPKQIIEFVQRPDMSLLDLHKMVESLKLPRNHSKILRKMQIIVQKQESFLNEENSNIRTYVDEDSISPMMIFGKLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M536</accession>
    <name>A0A8S1M536_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0510280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M536"/>
    <dbReference type="OMA" id="HQSWAYG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="140" mass="16421" checksum="C3668F02485A7D0D" modified="2022-10-12" version="1">MNQSMAKLYSQDGTGRDSYIFYDNGGFYPGNFKLQKTPQHQNWAYGTYSPQKTHFKPEKRQFYISDGTGRDTYVIRNVKDKAFFSPDFSFQLRKYDSQVLSPQYPYKLPPLKQAQLSIQSDKQKSLIQRLAKPKLRTQND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M855</accession>
    <name>A0A8S1M855_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0470159</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M855"/>
    <dbReference type="OMA" id="EAQMIQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="323"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="230"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8071394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="401" mass="47732" checksum="A64F8E9B4E307FE8" modified="2022-10-12" version="1">MLPKFQNSFAQDKNLQTLIESFNQSQKNPNALQRQRFLSYMPPGSIRPSPNGTQALNIFQEKVSFTLLANPSINSIFTNDQQDIILKFQIGNRNFQIVTLDRPMKDQKEEESNQNPQIIYPYQQQKQKIDFKLEDTDEIEVNGNDNSLTISTKSIIKYTRSEQNKKPIQNLHQEIQNKNEAQMIQIQFKFLREWYVKLGNNQYGAKKVLQEYLKSLLIPKIKFLAFDQQQSIPEPTPQPPQPDLDKKPQKIEEQEPEEDQHYLIPEKDLQNLFPHVTEEFVQRQREALEKKNQTTEEKIKRLFEEVRIRHFKSQPYNAEKNVFKCPYHKCQKFYKKPNQLKNHLKTNHQKLSEIGFTIDDNGEYKYNERILDYCQLLWKVYPNFVKSVINEMRQRKDQSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9Y7</accession>
    <name>A0A8S1M9Y7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9Y7"/>
    <dbReference type="OMA" id="WYFDAMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055304">
        <property type="entry name" value="CHCHD2/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523:SF2">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2/NUR77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="14063" checksum="EC67983B70A7EAF7" modified="2022-10-12" version="1">MPRRSGSSSRSTASPSRSTATAARPAPQTQAPPQAPARSGGMFSGLGSTLMQGMAFGAGSEVAHQAIRSVIGGSGHSQPAEQPAQQQAPQQQQQTCQSESQMFSNCLQTNQDITRCQPYMDIFKECQKKYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAT6</accession>
    <name>A0A8S1MAT6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0580119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAT6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035837757" evidence="1">
        <location>
            <begin position="16"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="342" mass="37790" checksum="BFF7E660B26B3CCB" modified="2022-10-12" version="1">MRTLIALCLVAAVFAIDTNKFAVLLQAGTKGNDAVESVYNLLRDLKTENVNVQAAADKKNNTDEEIFTQVIGDLTNVASLNKQQWEALGAVRTDVEAQIRDGYSWLAWAEARLAEIERRNAQLQDQRCWANGLFVRSLADHADAVAVVQLLQQDVAGWLTNNAGVELVQKAETIADKLSAYSHLFQQDAIKKFQSLAEVKRDGTTGEQVLSILSDLQAELEGTLATLQEQEIHAAFALAKYVSDTNAEVAWLNSEHERRTGLVEKLETQLPAVLAQQAKALKLWKDSLNAVAGATADLEEKREFYASETARRQEENAIIDVVIQLFKDQVRALASQTSLGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJY5</accession>
    <name>A0A8S1MJY5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0600252</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJY5"/>
    <dbReference type="OMA" id="ERQRMTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18557">
        <property type="entry name" value="ABC_6TM_TAP_ABCB8_10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000604">
        <property type="entry name" value="ABC transporter B family member 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF1">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="161"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="500"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8078791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="761" mass="85978" checksum="E3D84CF42ED45725" modified="2022-10-12" version="1">MQNKLLDKVQEENASNKMSKLFKGSQNDYYVSMKEEDSHQIEMNKIQRKKVFLSGSEDEEERLPIPIMKQEDNKQIQVYQQPEMRKSDILDEVPSQENMDGLNGMAQFELSQINIQAGKKKNKTKKNKSKKKKVKYQALPFNWNTYKFLLHYPLQHLKLTILVNVLIVLSAVAQMLLPWILGRLIDAITKNVQQAQSSDTDPINNQNIPNNVIDEELHTKLSKQFLYVLCAYTLLSLVRAFANQIWQEQIYNDMREDVLKSFLTFDLTFFNTYRNGEIISRMTNDLQSAKSAVSGNIILLIRNCCLTIFNIIILFALSWKVTLAILAPMPLYTVLTTIHTRLAKKFEKLGQEYQAKLTTLADEIVSGVVTVKSFCTEQFEIKKFHKQLDLNIKLAQKRGINNGCYQASSALFTQLGSLIVLWYGGKLAMNNTAELSAGDLTTIILYSIQLSTSSSNISESFAKIVSATGAFEGVLDMLKWESLVKEAPDAIDHITPTGEIQFKKVVFSYPNSQVQVLKGITLKINKGEYVAFVGPSGSGKSTIMHLLERFYDPQSGSIFFDEINIKQFKLKALRKSIGLVSQDPVLFEGTIESNIIYGTENYTKDDFEWATKAAGVWQFVSDKYKFPHGFDTFVGEHGYTLSGGQKQRIVIARALLKRPKILIFDEATSALDAESEFQVQSAIDELIQKGSKNITVLVIAHRLSTIVNCNRIIVLQNGVVAEQGTHQELLNNPDGIYRQLVERQLSGVIDSKDNDGDSEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKL0</accession>
    <name>A0A8S1MKL0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0580057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKL0"/>
    <dbReference type="OMA" id="IQICHSQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="756"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="756"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="769"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="778" mass="90375" checksum="7CC1CAA4E826A823" modified="2022-10-12" version="1">MLGKKQSSFIGFNPFNKIVKENPSISTRIINKLKSLFQINLNPSSYIKKELISQQTIYVPVDLEIVGAQTIEHDSIQICNSQNSIKQTRNKFMSFNDHIKDQQKEKVQQNELSQKVQQKENKHMKKFNQLQYSFDPVLPEDIDIAQTKQNNIIHPKRKLSSQQYKKRKLDQKKQQVGIKFVFKDHIYDQIQDSINESRIKEVPEQMTDDSLKNNSLLVSDLKQEPKNKEMIIIQLDDEKQKQIEPPMLTMNEIIVNQSKGLKKSQSMINLNEQESKSITYSPKIHENSQSRNHSDIINKINETKQIDPFNDQVKKDIIKIDKLTEFSVQPQQNVINFIVQGSEKQDIIKITNINTQLQKELSEEKKISEQKERNINNFSDTPLFGFGKCREGQQGQVIQNKINVHSSIFASYDSKDLNLLNSSNQNQNLQQNQFTFLQNKSNISVIQDQPSNSNEKNQKDILNRQEENKQNNGMYNNTSNNQNEQFRPMKQLFQNLIFQSQSIQGEQRKEENNQDQPEDNQPNSIGLNRQLKSNNESQKQQNKCNFSSTDQLIKQVTTPVLQQSPKIDQEQIISYFSNHSQQQNRNNKDDPAKIQTSQFSDDLFKLQNNGTSMFNPQSAQSSLFSFQQLSNQQNQTNQFLPNFNVFQTSVNNNMNMDNNSMNNNNNMEITNTPLPTQQFQFTQQLLQTQNPLGSFNQPLSESNNFYPQIQQQIQSGFNVIQFNTTNLKQQNEHQNQLLQIPQTNCIFTQDSTKLTNQSQSLNSSCQSRNAKRKQRIDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNP2</accession>
    <name>A0A8S1MNP2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0660201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNP2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8081850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="146" mass="16366" checksum="6B426382E395499B" modified="2022-10-12" version="1">MSNAFKSAAGNTLPKNDLGQTGQSFGGSQNPASLKGKLMSLEETIKGIQDEMNFHKKEVQILKSEKDTLESVLSMKTQDVKKTLTNELMRIEEEMKRHFAHQKAENSRLQQQITALKGEKTALQQQLLGLQRRIAELELQVGQEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSJ8</accession>
    <name>A0A8S1MSJ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0620159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSJ8"/>
    <dbReference type="OMA" id="ADLESKX"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035926266" evidence="2">
        <location>
            <begin position="18"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="295"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8079705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="347" mass="38756" checksum="B7953CCDA04F46AA" modified="2022-10-12" version="1">MKGIIAIILALAVVSNATSTKDQILALLQTGTKASDAIDTVFGLLNDLKQSNIDAQFAADQKNETDEWIGAQTIEQFTKIKTLNQKLFQQAIENRAEYENVLQQTKNYLAWNEARRDSIAAKIDTLQDNQCFSNQLFVKSIKHNQEALEVIRLLKQDVAGYIINGDSFEFTQVQAQSVAEKLKQYSNLFQEHQIKSFLALAQDEQVSESSGHGQTLAERVLGVLEGLESELAASLENLKQNEINASWELAGWVSLSEAEIASLEVEYERKQVFADRTATQIQAALAQQAKSKIILQESQDALDQAQSDLEAKRADYAEAKAKRQEENAILDEVIIMFKKQVASWSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSV2</accession>
    <name>A0A8S1MSV2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0710195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FHQIARM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026306">
        <property type="entry name" value="RSBN1/Dpy-2/CEP530"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354:SF11">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354">
        <property type="entry name" value="ROUND SPERMATID BASIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035813039" evidence="2">
        <location>
            <begin position="20"/>
            <end position="3064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="708"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="605"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="725"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="811"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="830"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8084137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3064" mass="357297" checksum="2C40C1E23EA08433" modified="2022-10-12" version="1">MKNAYVTIVIVSLLTFASSKLTLQQAVSLNMQNLRCQDLDDINIMISDTIQWKEAIELNNQLGDDLNGLLEVEKIVKNTKQQLLHQQSLMEIEMPAKQEFQAKFEIDLAKIMKLMQQMNVKQSKKEKLDLLTEIDVCIQATKQKMQQILSQSSKATVYDKEQLTSNLKQLLQIKQQCQNQQQESKQKISRTNNHSQANNYDEYDYDDAIQQVLYDSDSDYDYDYSNYYDYDDYDYNQGRQYQSKSKKQSNQRTRINQKQLKQPKQLRIKKQIKKKRDQEIHQPNIQKQNYNEEQFQDQDFSDTDSDQQYQNPTFLNNEEDDNNYFPVQEQEEQQDQDFTDYYVEQDSKPYDALDPQNQYNIIQDSHILEDQDVQNQEKNNDDLKEINSFDDILDHLKKQTDNNYQQSDVQDDQEFQDNNDNQIDIQEEQEYVQQTDQDVETIQPIQNQKQQRFIKLNNDDYEQQQDFDYQIEQENVSQNISPSQELSTLDEYDNNYSQNQDNEQNNKVITDKVNLDDVEQEDNSEIEYIEDFNNSEIQYEYVIQDQQNNESSDSYNDNDEQNQTQLQLNHIQDEQTETIQDISDIPDSLKQTTQIKVQAEEFKLSDQQQQPQQQDQQQQSQQKDQQQQPQQQDLQQQQQQQKDLQQQPQQQDQQQQQQQDQQQQQQKDQQQQPQQQDQQQQQQATNSQQQQQQQQQQQQQQQQQQQQNTLQTQDQNIQTKDLPLTQQQSSQQSQQNQTTNNNQQDQEVINQQAKQQETQQVNNQQQPQAQQVNPLQSEQPSAQQGAVQQVQQQGNQQPSDTQQQNSQPNPLQTSEQQDNSQQIKIPDQLTEPQTQQKDPSQQPSIEIDQSQLQPQKVSEQKPSLPTELIPDTQSQIPTKGLDENPDQIIDPVKFVPFKHKPLQSTANILIRQSKFYDSTIEGKASYEIQFSNKELQDSEEYGYGFWMRYQSTLEHKMQQNQYHFLSRLTSIKDYLDFQNYGDRTLANFLVDNSFVFATYDYVGREKNKVAQISLNQNIDGRWYFVSFSYSSRKQKSVGFILTYGKGREFQRIEIPCTHAPPFYMSMTVGGKHLHYPGLQGQFANIFYDIEAPALIDNEQELFNLVETMSFMPQGLKTYHEEVIVGPPVDMDGNTNCSFEKGFKDSFIYEHYAIAGWFRWVDNLDVKELNSFQIMNLRSNKQRLKDKRELGDRALEIHFIRGLQNIGLNFHTYSVTGNEGKGSEMIVKSVPYTTNVWTYVYFGFNQEEKKAQGIMIKVGVNEEVLFEGLEHKNTNSLYFTLGGDQTVASFNGRVSQVGVYLGPESYAKSKKLDYNFGYGDGAVRLFQLVKPLVMRGQQDQYEISFDQQDSIINQILIQDDNNIRINGLSEYSIGLWTCWLSSFPKFLGSRSDFHQIARMGTQSKLLELKNNKLIQSVNGQVNNDIKDTTLQMRLGKKEYEFSTYNLISNDIKKGVIALDSQLEGSWNYLSFSYKRMNNNMGLAKGYVQFGIGGQVKECTIEVLHDFILEYVELNVGKSNIPQFNGKLANIQMRLGNGAFFVDIAQYEQFQKLEKPILGLSQAVRKSIQLLGQETEMIKVKETENLFEYSQYSGVNEYALSGWIKWGGDQKQGGLYSILMMVQKKQQDIKPGQSDSQLYVQRTDKDYIFGTYNCNGEDCSQSQEKNIEFNEYYNQWTFIYIGYTQKQKKLFGLCKFTFNQQQQTFQEINRILLSTFTIFLGKKYQLANQWLGSMKQWVLNVGDGSYLENGYEQSETVELNFGFNSGTDHLKLQTANQEIDHSNKVIDSQADKQNIPWMIELNEQSDVKIEGVSSYGYGMWIRFRYYGASILYSQPKWMGLSRLTTNKDYRDADNVGDRVLMILFGKGEDEKSQGIFQFSTYTIGQPNIFSNLQYQMEYESEWVYIYYSYKRISQTQGVAMGYVGRMDIVDELKIDALHNPISNYAQLTIGHSGKYYPNFNGQLTGIKFRLGNGAFVESKNDILQRIKNTDIMPNVPYDVSHKYTIIDGKIDNYKVIPNNPQMIDFPTNEYSLALWYKQFQKINDNKETVVRVTQNPVGKVSDSSWVGDRTLAFFKNNKDNMEFSTYTLLKGMSFGIPYNCQIPELSQHNWSFIYMGYSKSKQKFYYYLSVDEYICKSEEIILHAISDRLWIYVGSDNIQQRFEGNLAQIALTIGPGSYTNSKIPFLAEYLVADKLFPKQKKVTWEKNEQIMLVSDGDETISSLKVELFNGFQYPFDQMQEYAFGMWTRYLTTIPQRQLTKPALMQLARLSINKQTTDGVIKPGDRVLATHIVFNYYQISTYDLNTDAGIQQKFVKYNQLEGIWRYIYMGYSKDIETISCYTYDTTITEMKIDKILHKPLTDYLLFRIGKEDTITGFQGSITKIMLSIGPGSYIKQQDQFKAQIDTTFSLPFQLTQEYIDKEKHGKFEIIEDVNQIDITNGIQFEGNRWSGISEYAFSGWIKPTGTGNTDCQLILRLTNNNVEILNDRKSQGDRTLHITICNTQLIKYSTYTLVDLKDANEPKFMDGQLELNDYNYAWNYIYMGYNQRTSEVHCLLHTLIEDKPITWEQVQHYVPNYLGLYIGQDKFTNKFIGQMNKWTAAYGQGGYVSLKKNGYQQQLPYYGLVQPNKQFQWNSNKDTVIQTPEFIVQEFTNEIDSVTEYAVGIWTRWLTDFPDHLVERKESHSIFRLTAKKEHQDKSQIQDRVLSAFLNKGSYDFSSYDIATNNFAVNSLVAYDQIEGSWNFIYMGYKDGQTVGIIIVRDTAKALKVELTTKHIPLVGYAKFLIGVSEFGHGQFHGWLFDPRLYLGQGSFINESQKVVDLLQKIHRKLPLPAVDLADFKWTIPQMDTTENINADVINYKFLDKQESLEYSYGMWIQQSPLLPGMPIVPRIISRLSTNHPQFMKDQQLGDRTLLVSILNDKLSYNTYKLLDTPEFEQKVEEIQVEQLQWTYVFFQYKKGNALAYALQVKQAYQKKIEALHVIPNVFYFYIVKDQNFAEFYGKVSDVKVYFGSSSYFEDPQVTIEKWPFDKKYLQPVSESPINNQQITSAKISRNMDIKNEKYIGDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MU51</accession>
    <name>A0A8S1MU51_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dynamin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0640194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin N-terminal" evidence="1">
        <location>
            <begin position="827"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1278" mass="151491" checksum="2AC700ED13C71192" modified="2022-10-12" version="1">MEQQQTAQGEPNIEQDINTVFDNLEYIRTNINCEIPQIPKESIVLIFLGEFEQKFKVFFSHFQIKQTIKDEKYFKIIRIEKLGKISFLLNFPLILQQDDQNNEQALNLCISKIYAEQAFQKMENCKIQLIVDVNQLNGDDDKAKFNSIINDQVFYFGKYYFKENTFLFLRPQNEDEWNLQTIKKNHQNKQKNEKEYQFNCDSKVQKDYEVLKSIFKATSNFSQLKVNSDSFLDYGKMNTYLKDFQEILAPQFDNLLKKFQKQLLLLQQMPIIQSQDDTIKKVGNYFKALQVDIINEENPLTNSFADISKVFEFINKVNKGNIAIELNQRIQQKINSKLMQQFLEDNKIEKISIQPFFNDNVQNKLNKIIDFYKIYQILLLQTTPIVEVQNLIINLQFWCQYCEEAIENILYELNQIVKKIQEGPQNEIENGVYFIGVTKSGKSTIINAILNPDKLEMTKILGQKCYEIKQGFQTKFKIGQGQVSKTQQINGIYIGKKEELDNIFYKQENNQQINXKMENCKIQLIVDVNQLNGDDDKAKFNSIINDQVFYFGKYYFKENTFLFLRPQNEDEWNLQTIKKNHQNKQKNEKEYQFNCDSKVQKDYEVLKSIFKATSNFSQLKVNSDSFLDYGKMNTYLKDFQEILAPQFDNLLKKFQKQLLLLQQMPIIQSQDDTIKKVGNYFKALQVDIINEENPLTNSFADISKVFEFINKVNKGNIAIELNQRIQQKINSKLMQQFLEDNKIEKISIQPFFNDNVQNKLNKIIDFYKIYQILLLQTTPIVEVQNLIINLQFWCQYCEEAIENILYELNQIVKKIQEGPQNEIENGVYFIGVTKSGKSTIINAILNPDKLEMTKILGQKCYEIKQGFQTKFKIGQGQVSKTQQINGIYIGKKEELDNIFYKQENNQQINNQNEQDVFSYEEILNKEQINQLKIPEKWFIFDCPGFDDNSNEQMRIAHRISLFNYFKKTKNIVVFFIIDISVQQVENIKNTFDPIGCLLNNKNDLQQNFQSWTNLILTKVKKDQRKEYIKDWKLAYNEQLEQNYSFYKQLFADDERCIEFLRPQMNMDLNELRNSIIEKAKSQLQQEKKYNPSFEFTLDEKVWRLYEKCMPKIQMKLEQLLQLFFQDLESYIVQSDETIKEKKEQLQKFLDCLHNQQTNIIISEINVNNCISLLQKLSQWIKGNKYTQKICFFLEAYIQDIQAILKISEYYNNKKIVPFKIKIDIQVTKIKNILKIIEEIEKQYQNCQSKFYCVIGLVLQNAQNLLHNMLGIKINRQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MV08</accession>
    <name>A0A8S1MV08_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0650078</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MV08"/>
    <dbReference type="OMA" id="QYERNQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001546">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001284">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="67"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="402"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8081156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="57773" checksum="708590C0F1F6E9E5" modified="2022-10-12" version="1">MGPCQSQSKLKSSKNLQQPTLIRKTDQVLTNNNPSLILPSFSPNPEVISEPYDDTKKSLSQLKVYKFSYQRFYSEITQGNMKLSSNQKLVQHNLTGKIRVVEQYERNQQNEEFITQLKCYPLDNPRIVNVHELYIDEESFYLIMDYCSGGQLSNYYVDKSIIPFNEVEIASIIYQAAESLRYLEKMGLTHGFLNLNSFSRVDTSNNFQIKLSDIRGLFVKPELNEQNVFSLPPEACNTKDQNVFNHSRDVWCLGVMAYQMICGYPPFEGNTIESVKSQIKKMAAQYNNFQFDRVSKLCRQLIQRILAPIKQRISIVALMNDQWFKIRIKQNDQSILNKLQQNKVKISELQQLFLIFMIKNICSADQQQLYTEFVNLDENQDGQLTKEEIFKVYSNRFASKADAKAFIENIFKIADVDKSGTIDFGEFLVAITDKPGLLTEENLKTTFKMVASSNGKLTTQKLRFHFSTCLSKLDKLFSENFANPQNVGYSEFELLMKQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MYY9</accession>
    <name>A0A8S1MYY9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="3">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0740217</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MYY9"/>
    <dbReference type="OMA" id="IRIRRHN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11610:FF:000017">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000119">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="9">
        <location>
            <begin position="614"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8085598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="984" mass="113529" checksum="4B80194F89C7465E" modified="2022-10-12" version="1">MLRFIARNLNNVYKFSELQKFGASFLGSGNAEYLENLFDQWYLDNKSVPATWDSYFRQVLESNNFDFAPEPQKGQAVSVRLDGQSGVRKLLSDHFRVLLLINKYRHRGHEKSQVDPLDLEHISQIGKVKGYTKLDYREYFAEEDLDREFYIHAIGSTGITKDKQMMKLRDLINYLEKAYCGKISYEYMHIQSNEERDWFRDQIEKYDEFMPSKEQKLKTFERLGQEHAFSTFLQKKFNTSKRFGIEGCDSMISGLQSLIDAAANAGAEHVVFGMAHRGRLNTLYNVFQKSAEEIMVEFQDLKSTFNEDIWGNSGDVKYHLGSVHNVFFGDKKLRLEMLPNPSHLETVDPCVYGKVRAIQDYFQDSKREKAFGVLIHGDAAVSGQGIVYESLQMADLEGYKSGGIIHVVSNNQIGFTTVPKDSRSGLYCTDIAHAISAPVIHVNADEPELVDKVFKIATEYRYKFKRDIFIDLVGYRRYGHNEQDQPKFTQPIMYEKIDKAPPVFIKYSDKLIAQGIVTKEQVDNLMKTHEDNLELAYQKSRKMDYNLKDWQPVPWEMIKVPVLWGRIKDTGVPLNVLKTLGEKINKIPNEFNAHPQIRKFYEERLSWIQKDQPIDFATAEALAFGTLLHEGFNVRLSGEDVQRATFSHRHAVLHDQKDPNGQDYVPLHNVIPKGQEHRLSIYNSHLSEYGVLGFDYGYSITNPNTLVLWEGQFGDFANGAQIIIDNYIASAESKWDVDSGLVMLLPNGMDGQGPEHSSGRVERFLQLSDDDPAVFEKNLGVRLTRQMRNSNMQIVQCTTPANYFHALRRQLRRDFRKPLIAMTSKRLLRLQAAKSNLNELTTQFNQVYDEALPEFLVQSNQIKRVILCSGQIYYDLLKKREDLKQNNIAILRIEQLAPFPYEFLQKIIGKYNNAEFVWVQEEHMNYGPWAYVRPRIQSVLAKTTNISNSPIQYIGRRPSGSPATGFHQLHEKEIQTLLQKAFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZU9</accession>
    <name>A0A8S1MZU9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein disulfide-isomerase</fullName>
            <ecNumber evidence="4">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0760209</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZU9"/>
    <dbReference type="OMA" id="LMWIENM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF132">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="139"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8086447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="248" mass="28923" checksum="8451C3A29AEB857B" modified="2022-10-12" version="1">MLLFLIQVAQAITFSREVASQIFESGKPALILFYENSHQDQLNEFKKVKNDQLLLIEANNSDKSVNKLIQILQIHQLPAIKLLKCDGNTWQDVQTINYDDQILIEKLNKFISRYQDGQLFYDIQSEDVPGRQMDEGIEIVVGHNYDEHVQDTNRHYLIYFYSQDCDLCSSMLQNLVRLNNQVRTGKTSIKPNQLKIGKINGELNKIHGLKINKYPSLYLYKMNDKTPIEYKGDRTTEGMLMWIENMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0I9</accession>
    <name>A0A8S1N0I9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0640051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0I9"/>
    <dbReference type="OMA" id="VCESIML"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="25441" checksum="CC01C9C6D51D4B7E" modified="2022-10-12" version="1">MDIYNFRFENYQPKQSFYIINKMLKFLIAIQFVLIFFRIFVRNQEAFFLSDCLNVSMMILTYCQKNYCLALFTMFYIFSLIYLNTIFFLQFLEYKLPLFIDVNRKFTLLLIFSTLIYVFESIMLYQVYKEYKGIIYDINQAYLVQQEQQNLEVEIISYSQEQSASQLINNQQLQAEQHIENQSIYEPNRADELIQSRINNQRQEIQEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0S5</accession>
    <name>A0A8S1N0S5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0S5"/>
    <dbReference type="OMA" id="QCTQEEK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000078">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="126"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="405" mass="48056" checksum="39077B42C3CBE4A1" modified="2022-10-12" version="1">MGQLQCTQEEKNDNIINRLELDFEKQENIKQDERANSFSYVHAIGKYLNIGVNPKLDQIWEEKLNSFKVYDEPPRTQSNYRNSLTKPKNIEQFFAECPLITQDYEYYMLVFFIQEKYEFKCYGLIGLPQKYRWAYWKVITLQKDIMHKNMENQINDSSYYIKKDINRTLISELFKEEQVVEKLDYVLTNLASLYPKVGYCQGMNYIAALFLMVSGAKEQETVCVFAELLDSSFYMFNLLFKEDLPLLFIIEEIIMKLMQKKMPKVYNHFIECNISPSIWISKILLSGFVYLFELLDCVLLWDYIFIKGTVLGYTDLILAMVTINQDILLTKDEADLSIFFNFQEQKIKCAEKIIKQALTQPVQEKEIREIMKKTNQKLELVELFKLFGKEGFEKQYKKYSQGIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N377</accession>
    <name>A0A8S1N377_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0790124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N377"/>
    <dbReference type="OMA" id="NKIVQVP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="81" mass="9178" checksum="95AADF487D29A11E" modified="2022-10-12" version="1">MGICGTSSMKSHPSDKLIEQNKEKNGNQISQSKTVRLFDYNQNKIVQVPVLKPATQNNLYFKRQAQSPNQNQDTVQLLTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N496</accession>
    <name>A0A8S1N496_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Scavenger mRNA decapping enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0790020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N496"/>
    <dbReference type="OMA" id="HVHINPI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000059">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008594">
        <property type="entry name" value="DcpS/DCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978:SF0">
        <property type="entry name" value="M7GPPPX DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05652">
        <property type="entry name" value="DcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="31994" checksum="A8271C3C0152B0DD" modified="2022-10-12" version="1">MNNELLQQFRPSQMINWDDNTKKLILLGNLNNQCAILILQKKPFEKEIQQLQFDQALQYFHNDIYTKYNCQMLNDIDCELIYPANQVHIDKYSKSDSIIIEETYDMYKEQQIIQMPLDWVYNILEKKKEVENIVFENQTFLILKDYVFVNSKSLDDLHLLALPFQRDIKSLRDLNQDHISMLEDMYTEGLRIIQEKYKLEQKFIKVFVHYLPSFYHFHVHFTHSSHIGQAFRDIPLLQIIQNIKLKSNYYQSVALQYITKVRINQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N515</accession>
    <name>A0A8S1N515_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0810060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N515"/>
    <dbReference type="OMA" id="NIPQECQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="28"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="538"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="538"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="574" mass="68310" checksum="5FC448EFABB6420C" modified="2022-10-12" version="1">MSQQNSNHLYLVNSIISAPTDQYQKRQFHMVKLLGRGTEGAVYTVKPINCQYSKNMALKLQLNIKDSENFFIEDLMSYQQQFDQDPINASGIISIFEKFKYQNYHALLMELGGLNLYDFIQTNKNLSIQERFAICFDLFRPIYFIHQKGKFHRDIKPENFIQVGNKFKLIDFGLIKGASKDLQQTKGIGSYYYQAPELVEQRTDYNEKVDCWALACVFYELLSGETLIKSESEQGIKQEILQFKFQKEQYFQRIDKIQIPQDCKEAIKKMLDPNQNQRLSIFDALQTFLNYQENQQPNYQNAQPPKNQNYNQQVNQTQNNPLLQTQYQMYQKQQNIQPKVQQQQLQYNNIQPQQLKQQPSLFQQQPPLLQQQPPLLQQQQTLLQQQTPLLLQQPPQLQQAVPNLLIEQYAQYFINEIINQILPSQNQNQRAKQEIQNKNYENTFEIEFQNAEKDILDVCNEMKKDIEIEINVKKNQEMEDLQKKSEAKIQEQESELEQKEQVITKLQKQYQDLEQKYQDLEQQKQVLEQQVLEMKQQLQRQNQNYQQENQIQDHKQQQDPNQQESGFMESENKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7L8</accession>
    <name>A0A8S1N7L8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0810173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YLYEYQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1065"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1384"/>
            <end position="1406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1586" mass="190452" checksum="C08FEC5F21D0C794" modified="2022-10-12" version="1">MMFYQIWMDLKESMNTSYYTSFIYLSIGAILNTSPLLEIYLKIADNDNYDQNQLYLILGSLNVSKYMLNSSILHSIYIVIIFCFNTFIIILFMILYYLKKYNVQTNIAFTVYGQIIGYLIQPYFWSINGFLLQIQNTYWEQIQNLAFILLDFIAIVQQLLFLITLILFLHYDHSQKKNFLKINSPKTLIFYIITIFFNSFLELFINSSDQLNIAFPIVLVILYLLLFTQIHLDLPVFQNKQINNYFQIIICIQFALALNLLVSYFINFFEDRYCLQVILTFILLIQLNSKIRQYLIFRSINNYSVTRIIDQKTIEYLKQNSEYLLLLRGIVQMHQSLCSNINCFYRSKFIYKISSKGNKKILINRDSIYDNIGRISNFFLKCSYETMQQKSQDQSISIQLIELLCYKLKLYSVSLTQIDKLNEDKLNLFQQINIMNIKLIISRLIKQKNEQAYKSKLPFDHLLQCESYIKETVQIIINIFGYQQQFWNYLLNEEIRLIDELIILQKISRLIKKFQELQKQILNLHYKQKVPIRNRQWFNYFFFKLFILNKKLKLQDLEILPDFQLNLDQILKDEYIDEKSSDEEPINYYQQKFLISGNTFIIQINQSCIVKNCTNNTFQILNYNKQSLVNSSAVILMPQMFKQCHYQFINYFQQTGQSYNLYKRKKAFCIHDQGYIITVYKYLKYIYNYTSNQIEYIAMFRQIQTKQQYLLLNEDWEIDSCTQFLQDIIGIKSLSLFILAPKTIVFSEYEKFLTKENKEFFSLNAPNQDQNLSQLFYTQPSMIRGSFSSSNGQNSNLLKQDEIQRSYQNFLNFDDVINIKDQQKSKIKQVLFNLRVPKQKYELVQEFQTKLSELYKQALYTELFKRQLIVKNQFGKYQIVKSEFIRRCKIYQEIIRKEKFKIIKELFQKYTQSEECIDKILRIEGTLKLEKTLHSVKYILIKIVRTELIKENQKSYQQNCGVTLSFQTPLCQLISSQQSKRSTNTQLSPRRQYVQTYDQFDLYSNRESIGKVLLPELQVNQDFDQNDQNKNINFITCNSDLLKEDTNKIEKYNPQIVQYEFISRLIFILQILIVFLSFFFTSFIYNQNTIVQSVNLVDYLYEYQIIVAESYNAQIDIELFNRNYTYQDLTKGDSIGFESDEQFYEYQQNVTQKCGDYMYFVFLEPKFLKMLYENFYSNSNESGLDIQTVYQVNVYLLNMLSSQKFKEDFGISETFRKYMLIQWIIYSIKDQDSKKFLIQISYYFLYYRQFFKYQICYIQLDFNFKYRNNIMYNLLKFLKVLFKVQAMIPYREKVAIIEKISTSINQFYYVTNNEYGDLEQFSPRNKSYHSRQTTLRRKSSSRYGNEKSILDIEKKHKEKSDNKSIIKFQQLSLQYSLKKIVVKIILNLLLITLISIAGVITIYYTLKNIELFNKNQFMFNTQNAWVLPAIKEIFTSQKQVDYNFNDKNAQIILQFYFEQISSEYPVFYESNIDEIYYLFDNNICNRDNDIKLEKCELLINGALTRGIREYDYLLSKLVQKVLNPNDERYENITYSTIYQFNKLYYFVFDYYTFAKDIWVKISIETLEKHDQVSLILLNMPLFYNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCI4</accession>
    <name>A0A8S1NCI4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0720056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCI4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="364" mass="43409" checksum="EC583F319A861AF0" modified="2022-10-12" version="1">MEQPPEYIFASIDNCFDNSIVFYFRILRWDFILMVIFAILHLPQLIFCITENSIFNQFQLQLETFTVPNIDISNETAFLIGVLGDAIGTLLYFLSLIYLRQKQMDFHQLQLIQKIYVPFFNLIHMKYIQFHYEEQSIQREIDQIRKEKVRSIFQEIIISRYVRKTRFHQSLTDELKDITKELSQKFNIFLASDDLESIKAFVVFKSKVDRDLFYEKYNQDCLVRNGGLLRKKQCLTIILLAPLIVFENMALKNARNNTVKCTEYCLDNDFTRTAYYIITGFWIFIADILGWIFLELIINKEKQIYLVQEQKVILIRIIVYLICYTLLVPILIHGKQLEILYQSFKVIHQQKNDQFLENGLLIMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGW4</accession>
    <name>A0A8S1NGW4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0750144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000078">
        <property type="protein sequence ID" value="CAD8085934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGW4"/>
    <dbReference type="OMA" id="EMASTGH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022127">
        <property type="entry name" value="STIMATE/YPL162C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735:SF1">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12400">
        <property type="entry name" value="STIMATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="35024" checksum="C93B1F3B6B653406" modified="2022-10-12" version="1">MSTEEPEAQGCELLGGFGILVQSILGFLCFLVLVFKRHIEKPKRVWKIFLMDTAKQFVSALVQHFLNIGLALLLSSADSGDQCQWYFITYMLDCTIGMLINCLLITLFEALFQKLGQTQFQTGNYYTITYPQITTYSLSSEDQSQAKKAKPKVEVAYGVYVFQLTLWILIVVVSKVILYFFQLLLGEYLLIAVSWMFQPINQYPELELVIVLVIIPLIFNALCFWVQDSFLKKSKFKKKEKTAVLDALYEKTDQPVEVEIIDGDESCRPSIVGIELQPQQKNNDLVITETKSDIEISDQQTNSSNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NIX7</accession>
    <name>A0A8S1NIX7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0820148</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NIX7"/>
    <dbReference type="OMA" id="KMKQFDR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="256"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="691"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8088935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1120" mass="130776" checksum="B2B660F3E3D459F1" modified="2022-10-12" version="1">MKNVRESNSFSRFKTRQKSRSNDQNSQILKLQSQQYVKKLPPKAQQVSFHLLPESYQGSKTKYMVQQVIQKPSNKVLNDTHNYLSSKYQANKLINKNSFVKNHSKKIHSMSYYHYDSIQNENDELCMHYNNLSNVSFKEYMDAETRANKISSDKSQEIIEGKKLLSLGQTEEAQKLFEEILQNSNNPEARYLNGLCHLSKSEFSEAIADLSLLIQQQPLYKRNAYILLAIAYKKSNCPNDALNTLTLSIKQFNKYFDAYIYRGKLLLKMKQFDRALKDFTCAVEIQPKKAISYIGQADCYRYMNQPKLSVQACTQAIECQDSSFRQALIKRTLLYIDLKEYDLALQDIQAVLEEDYCDSEALYIKGFICTKKNQIQEAFLAYEQSIKHNNSKKAVSKSLYEIAKIKIEQRDFYEAFYQLSRADYLDVDEKILEKFKIFTDGVTFLMKRKFDEGVDTLTTLITKHQVTDFLKPLIFQYRAYGYFCQSQYQKALNDLNQLTSLEKPSIYNKLIAEGILAAVSNQFEQSQGFFLKAQKLMPNKMEPYFYKATTLVKFYSYLIPKDDIEKKNKFLNDAIKYMDMAVKINEQSNLLFYRGIVLFAQGRLDDSLIDLDKAIEKSEDHVAKHFYVRGLIQACREAYEPSLNDLTIALNLDEKLVDAYLNRAKVFLLLGDRKNAYYDAQKYKECKQNDPQTDIILGNLFFQIGAYEDAIQSYSKSISSEKSLQVLYFRAKTYIVIKELNSSMLDLQKIVEQSNDIHAIVDLNVLQQLKNTSTANNDQNIFQEALQCVNQILKKGAEGKIFKKSDILFYKGLFQFYLKQYDSAQQSLRESYKIKEDLQKKELGSMNDSDQYILDQLNQTQKKVEFKSKPLEEFEFSDRTYNLYEYYFNRSAIHLLLGQSESALHYLEQLQENIQQLEIQEHLSLFIQLLKDDQNPKSEINCELSKLTEFLIFPQHNRLCSIYPMIKLPLKKYKQNLQLRLSFCLPTIEIPEMNIKFDDKLIETISPTVVENKPEAPWIKRTAEGVIFTDNVQIVEDLDLQSTTRQSKKQTEIEDPEFQQFEDQLNHAIEKYDYSEIDQQDDIVEQQNQALDLQQLKQNLMLDSKIADKLNSILQKKAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJ37</accession>
    <name>A0A8S1NJ37_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0770039</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJ37"/>
    <dbReference type="OMA" id="CKFTQNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="122"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8086674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="480" mass="56521" checksum="5BA58BBA105F082F" modified="2022-10-12" version="1">MNQKYCFKSYFSQQCTQFWDNTKIDKTQKYLMEDLYYIQTPFETIPCNLRLNKKNLIMEQDDKQYSTNVENAGLELITSDFNNDQGVRITKGNYTFEFFGKIKSIFDQLKKTSIQFNFTQRYTIKKMIAKGTFATIYLAECKFTQNEYAVKCFEKRHINNEKDKKNLIKEMQILRLMNHKQLLTIYEVFENSNYIYFVQELLKGGDLHEYLERSEKLSELKISQIIQNLLNGIQYMHSQGVLHRDIKPENLILRQKNNLNDIVIGDFGLADFYKYDGNYLYRRCGTPGYVAPEILRGQPYDYKVDIYSIGVLLYILLSGRRPFIGKNNQQTIKLNEIGDINYDDIDCSSEALSLMKKMLESQPENRISILSAKQHPFFTNAQKRESQKTKISLKLDVKCIPQQEHLCVSPIITSRPLNRHSMMNNFTLYTPRQLPRLSHDVKSIIKTESNEIHRSTISRQSLSRANLRLNNQFTNLQMYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NK74</accession>
    <name>A0A8S1NK74_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0770227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8087044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NQYELIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="492"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="685"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="963" mass="112876" checksum="06080ABC5B0CB005" modified="2022-10-12" version="1">MSSDLDKKLCQKHKLEILTIDLKQSTADEDKYLCIKCLMEKIDIQNMALVEETKTMIKQMKSEQLTFKIKEYQRRIEIFKQIQSQVKELKLSINNTIDKVQSNLDQKITLMENELDESESKTFVSTFEEDIRILSKNYKGSFSFEVPKEFEKSINDNSYIDQIQQQLQSIINCPKLIQIKESLEKTKAENGNSEVKQIQLLNKQEEDPQKTPSLKIQCNKHGKEIIMFNLNPEKTQFSRLACVECIQSNDPIKYTTLEDANFKWNEYLGQTSDQVKRFQNSRYLQSSQIIEILQDIKEKYNSTISEIINKINTQYSMFSQNETNEFNDNMIFLMNTEQINELTEILSQNDKFKALAEKQFNIQKEDLNQLVLISTNFGQLLQNDLLATQKINKIFKDSSLNISNVKDQTDDIVQDDNNSIQNLQQIKQLNQQLQHFKLYSNILNEALNQYELIMQTISSLSNEFKDVQQQQFNHYVSKIEKDLSLMQKFTQFDQVATELKLLKQEKEQLQNQLIESEKLNLENQQVINDFKKHDAEQIKQISDLRNQNQGLLVQIKELGQQKTQCELILNEKQAELQICQQSNQKNEQMMNIIKRYIRSRSGNSLNYLXNNSIQNLQSIKQLNQQLQHSKLYSNILNEALNQYELIMQTISSLSNEFKDVQQQQFNHYVSKIEKDLSLMQKFTQFDQVATELKLLKQEKEQLQNQLIESEKLNLENQQVINDFKKHDAEQIKQISDLRNQNQGLLVQIKELGQQKTQCELILNEKQAELQICQQSNQKNEQMMKQLQQTLKQEWQFSQLLTFSTTYKHSSCSVTQNGKVIENSSGGWNCCMCDQMIPKNGVIQFAIKIIEISCIMIGIGFRDIVQSKGYYDCHSIGGGTYNIYNNGYCYNHDQQDKDNKKIAFEFSTNDIIIVEVDIQKKYIKWTKQLTNESFTLTIDTSKDLYPCVHLHNRCKIEILNQLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKV0</accession>
    <name>A0A8S1NKV0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0850089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKV0"/>
    <dbReference type="OMA" id="MRLQINI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="138" mass="15157" checksum="10E7DB1B7FD4AEFA" modified="2022-10-12" version="1">MMLYVTCWMAQYCYQAKSYNIADLTGMDHYQTWVIMNIAGASCLLLGLLISLAKIAIDQAALGYGSLGISLIGWILITIGMGIGSDTAQFKDTSLDYNIPVSNWFAMTGICFQIAFNGYEHSIQEDDSTGQTSKQVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQC7</accession>
    <name>A0A8S1NQC7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CSC1/OSCA1-like cytosolic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0900196</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQC7"/>
    <dbReference type="OMA" id="ICKCCAR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF83">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="652"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="720"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="2">
        <location>
            <begin position="240"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8092456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="810" mass="93981" checksum="6874751E26271A56" modified="2022-10-12" version="1">MFRQGPQKYQQFQDEQRVISNKQEFPQEVQAGVLGGKEGNSDFPSDQIPPDFNLAELHREAWNVGTTKLSKSQLRSLRLHQNTPNISEEEKLVGQICKCCARQVPKDRVKIDCNNQDYSFLGAGMPLYFEYIKACILMLIVMFVTSGDYNIITNIAFGQSCQSLDKSIGFEIDEKAVCIFNWVTGLSLANKREDQEFIDLQQMLNLISMLTLIILFQYFRKEQRAFDTEIDSNTYYASDYTILLKNIPTHTEGLKNDDFDQDLKDYLEVYIERTYEKPIEVLDYEEELRQFLQRNNRPEKKLSRVVAVNLCYNIEEQTILEQEKQSKIAQKQKLLPSLYDQGLLPGSEDINNNEQIKEIDNQIIEIEQKLEALEKRFVEGKDVKQYFLGQAFVTFRWESDVQWMLIDHRLSLCSRILGRKSNLIYRGSQLQVEQPPEPTDVFWENLHIQTSQKIWRRILGYFLTFIILGVCGGLIYWLSAIQAQSADEQAKAVKNGDLNPNYKVKVIAQLASISIIIINYILSIVIRKVSLFERFSTQTGFNISLASKSSLAQFVNTAVITFAISTWVTKNIYGTGGLVYNQTYVFISNAILPALIQLIDSSTIMKWLYQFFELRKGKASVKTQKQLHDLFERPVFDISTSYATVLKNMYVVAFYASVIPIGLVITCFALLFFYWVEKYNIARRRTIKYNYSSVMSREMIEQLEYVLPIYCLTNLWWEYVFLQSISIEAIIGVCFGVANAILPMHEINKLLFRMKAKPNEHLPINEAEVGFLTDYCRENPATAETERARYQERVTRHQKDKIAMDQMFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NSM2</accession>
    <name>A0A8S1NSM2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1000176</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NSM2"/>
    <dbReference type="OMA" id="HHIRNYV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="197" mass="23026" checksum="A18766236A7B8976" modified="2022-10-12" version="1">MNYIYHIKSPISLICIAPHKCQRKLCVECMYDHGVDTKQTVPINKFQDKAMKKLKDSKPGDTSKLNEQRMIFKVLLTQIDQMLKKILEELSQSIKQVYDQIEKENQSYLNLINENTNLAESSYTDIEKLVNIVDGPTLYNWNVEKNSYLIDLNKQKSLVGSAYQDFYRKVRRRDLIDSIIDQVSIQVISKGKKKFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NUE0</accession>
    <name>A0A8S1NUE0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0970143</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8095009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NUE0"/>
    <dbReference type="OMA" id="MIDPPKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17947">
        <property type="entry name" value="DEADc_DDX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="10">
        <location>
            <begin position="91"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="122"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="306"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="461"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="91"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8095009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="541" mass="62270" checksum="8B076C7CB4141690" modified="2022-10-12" version="1">MSSSEIQSDSEDLIQGQKDFDFYADLLNPAEKLRKRIQQSTQKQITKLVKSAKKNEKQQKKTKQIIQKIKNEDLQENNQPQYDPKIFATNTDFQQLKLNKSLIKACHEQGYKYPTQIQAQIIPLVLAGKDVLASSCTGSGKTAAFLLPLMQRFGNTKSQKYSKALIVLPTRELALQCYEMFQKLNQFSNSTAALVIGAVPIQQQEAELRRYPDIIIATPGRIVDLMKNSFSIDLSSIEVLVLDEADRLMEMGFEAEIKEILQQTPRDRQTVLVSATLKATVKQLSLLALHKPVKVSVDYVDGLAYGLKQYILRIDSDDEKDREATLLALLQKKFTEKTIIFVRTKHDCHRLQILLGLKDLSCCELHGNLTQQQRIQAYEDFKEGKYQYLLATDLAARGLDIANVKAVINYEIPYETSRYIHRVGRTARIGNQGVSVTICLKREVSQFKQMIKESKQKLFKLNFNLESINEIKQELKQLESKIKKIIKEEVFEKEIHQTEILAQRAQNLIQHRVEIMRKPKKEWIQSAQQKKLRNQIQEEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P566</accession>
    <name>A0A8S1P566_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">STOP protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1060065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P566"/>
    <dbReference type="OMA" id="RANFKQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8098131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="31463" checksum="24535FDF8D6E6575" modified="2022-10-12" version="1">MSKPRSTSTIEQRIKDLRMQKSRNYSHLDGTCVCADCLCGQCKCQMIQQPYDYPKILLSNYKREYMWKYSSPPKPVQKVEYQSNFEQVFRSSTYQRNFQSFNIQKTENFKPSSQDWKNDSPVVEMTSYRANFKQQPNNITTMLKPIEVDHSITLPMTQKTTYNKHFRACSSQLENDHYVREAHYKRHTNQTPNPYLKTSYQHFYNIVSQSQTNAILPQQNQTFMPSLTGSQNRQSRYQSDYKKKQKLKCQARQFLEEYIISHQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P687</accession>
    <name>A0A8S1P687_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1070080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P687"/>
    <dbReference type="OMA" id="FIKISCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8098516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="282" mass="33716" checksum="9282B2AA7795FA75" modified="2022-10-12" version="1">MIPKVILLTQKLFENVDQQQQQSQILPCNYQHDIDLIVDQIKYELGNVDPKIIRNYSQKIIELQSISNEIQDIKVQLDIYFQIPSNLKKKQAPLIYQENKQNIKQIKLLIQKQLNDLSELSDVTDVENYMENEYEKIYIFRLSQIYQNEIDNLQYQFLQSQINNNVNFNKLNEKFLGIINDQTFIKISCQIHKYIVLLNLIDEYRDKLFEIIKSKSFSTESIIYQKSKQKARSLQYESISSLDLIDDENKINKRQHPLFKQEDIKNKRFENYPEACVKCQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7E5</accession>
    <name>A0A8S1P7E5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1080108</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7E5"/>
    <dbReference type="OMA" id="HDECIIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="6">
        <location>
            <begin position="145"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="358"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="600"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8098980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="728" mass="85588" checksum="A2FA8414D27AF6EF" modified="2022-10-12" version="1">MIIRAIITESQYRIHYLILMIFLQPLNHYMFYTLQKLECTQQTQTQFKQESIIQQNEQLICFSNDSKIDNLKCDLNESVIVNQINYDLESPKIQQEHKSIIVNRETIQQRIIIKKKPINIQKTTTNSSHSFQHDPLKLESHIDSKYSDLVAIMSNLPFGIIFVDQQLKILDYNQKVIQLLGITNPDNIIPFLDEAIQKSGETQEVRSAKKIKKSMNKSPKKMTYYKQPSISLQKRQFDDQIPDVVSQYYNCGSQPRMDNESQQEGNLKSVFQKFQKIHQSQQTSTSRETYQFIIRLDSMNASQCKSKYKSLKLKIFQIEENGKWNQNVYLIVLENITKREEYKLLNHKYKFQQALLNSLCHELRTPINSVLSQLYALKDELTIDLLESHLNPAIVSTKRLQYQLNDILDYAQIQCKALTLNKSYFKLEEIFQQLQELFYFECIQKQIQLIIEDDHQIRLYTDKERLLRVFINLLDNSVKFTDKGGTIKVQIEILKDQYKFIIYDDGQGIKDEIIEKIEEEAEILFQDSIQYNSNKLGLGLRISMILSKYLYKEQKFKIDSKFNEYTRISFGLSNLIENCSLEFQMDRQLSRLEKYCECSKILIVDDIICNHFALQVLLKKFKVKTDSAYNGDSAIELVEKRLKQQCCQTYRIIFMDIEMPQKNGFQASSEISQKLKQNSLHDECIITMYSAYSGDDDVLIATQCGMKERISKPTDIQKLEHIINKYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P9N4</accession>
    <name>A0A8S1P9N4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1100054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P9N4"/>
    <dbReference type="OMA" id="MYQIHKK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="247"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="36167" checksum="A1F4C762049C8058" modified="2022-10-12" version="1">MSNLSLQSQSQLPQIKNKLHVGENLMYQIHKKALQEYLQSGNIDQRIVSMNLLNVSQLKNEELNVLQQSLSSKNQKLEGDAGKILSKFRPKVKSNGFPLYFKKPDYAHEDQELNQLEEHLKIVLKSKLNTCKVFNGTYLVDPSIQDLQKTNQKSKQPQQLVYFPAIKRQPSQATLRDSQQIKQVVNQRLHDSELTLQELRDRYKESKLSDFQKYFFQLIDEGDVGELQSLFKQKEISQDILNSPNHKGYYPIHLAIKYKNPQLFKLFQQNGADLTVLTRKKKSIKQLLEIYYNDEIYELINGKKKGKLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PG45</accession>
    <name>A0A8S1PG45_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anoctamin transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1160144</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NTEFLIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF73">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="786"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1220"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1246"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1325"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1393"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1613"/>
            <end position="1634"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="527"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="951"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="8">
        <location>
            <begin position="1202"/>
            <end position="1651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8101811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1664" mass="197568" checksum="A6A8EBE3377FCA28" modified="2022-10-12" version="1">MQITQTQLLEWVKDGNKVTLKKHFEDELKFSQNKLSDFFTMVDDQKRNVVHWAAYLGQYKLLKWWCKKYKLMINLNKGDMHSYTPLELASIKGYSGEYNPEQQENTIKLLLEHGSLIPQSNTSKPNPLHWAFYYGNKELVDFLISKKFQLQLETDQQGNYPIDYLFLENRPDQYKKEVSDIFENIVINYAQVTTKQTLNRKPKKKKLRVPKKKKNRQLRDGKQHQATVVMSQLPLIQTQIKEDDSTPESEPSKNGIPYSNISNYHKSQTSNSFISKNTIPKSSQNQTQSQQILIKFQQRSCCKKIQSKNLDEIADVPLYDDSRIEMKEPEEEKIPEIKVFNQNQRNSNSCKLITEKNQENQFKADECNIIIAEPPVLQFNSETSPPKNFDHRRRHKSNRFKSQNEDYLEKIDLKEQEKFYIYHSKAYLTKNEIYECRLQYWSARQGNTEFLIYFLKRRCNPFVNVYQGFNCLHIAAFKGKLKILKIILEADYEYYDYSEENNQKYKKQIKDQIFDKKECINILTDQNPSNALHLAIEQEKYKCMKTLILHGVSVDTVNSRCLKPFELTFNQDIMEYYKENIQIKQQMSSLTELGYQYVIQTKGTLSVEQDIVYLQLQNIRQTFKDRGWSFEFMIFHAPNLDKLEMYNDNAQAKKVKSLYHYYVLKLPPDSIYKLADLYQISCYNFQTKYICQFNYDNRGFFEFPKDLQVQMLILNTLNDEFDVDKFVLEKLIISHYPLEDIQKCEKITQFWDEQQNNCIQDSIRYETQQIALRPLHAIASYFGPVVAWYIALNVQIIGWLMIPSVFGAAXKNFDHRRRHKSNRFKSQNEDYLEKIDLKEQEKFYIYHSKAYLTKNEIYECRLQYWSARQGNTEFLIYFLKRRCNPFVNVYQGFNCLHIAAFKGKLKILKIILEADYEYYDYSEENNQKYKKQIKDQIFDKKECINILTDQNPSNALHLAIEQEKYKCMKTLILHGVSVDTVNSRCLKPFELTFNQDIMEYYKENIQIKQQMSSLTELGYQYVIQTKGTLSVEQDIVYLQLQNIRQTFKDRGWSFEFMIFHAPNLDKLEMYNDNAQAKKVKSLYHYYVLKLPPDSIYKLADLYQISCYNFQTKYICQFNYDNRGFFEFPKDLQVQMLILNTLNDEFDVDKFVLEKLIISHYPLEDIQKCEKITQFWDEQQNNCIQDSIRYETQQIALRPLHAIASYFGPVVAWYIALNVQIIGWLMIPSVFGAALGIYIIITEEVNSSIVPFYALLMTLWSTLFMEKWKNRESELKFCWDMHKFRQTQPQRVMYTGQYIINEATNKIQIYDYFTTFKRRLIAEGPVILIGIAIIVLSFYAFNLWLQEWKDNAIMPIVINSLNGVSMTVFCDLYKRLCTSLVTWENHMYESEQEYSYILKVFLFEFLISYVSVVYVAIFENDASQLSVSVASIIITRGVISNVKSNFLPYFLFKQEKRILEEKFQEFKRIFTQKLEEKKQVPNICNSKFSNDKLNSEIQLSFLQELEIGRIKQPQKVLYDEYTSIAIQFGYTTMFAPTFPAAPLFFMINCYINLRWSIYNYQHILKRERAQAADSIGIWLQIFEIMNYCATFMNCIVIGTVNKEQFKGIIGNQNALVSAFFLAAIEHILLLIKYILDVSIPDCPYWVEKELRRYAYLEEKYLKNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHC4</accession>
    <name>A0A8S1PHC4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1180144</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHC4"/>
    <dbReference type="OMA" id="HFFENAK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035760188" description="Transmembrane protein" evidence="3">
        <location>
            <begin position="16"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8102506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="37090" checksum="FA4BFB6ABA71DD5F" modified="2022-10-12" version="1">MLQILILTFISQASQQTPQFNFSQFNDPLKMQQIYYLTRFIELEKSMNLTTQQAQENANFALELWVNTENFLNKTQKQMKNQSILNIINNQQFQEIIPQDQEGLQATQQYIEQWQNQTDDDKSIILQYVVVNINQMIQSEDPTKYNQTNLIEHISNQTFILEQNQKNKNTTQYISPLTWQHANVSNEEMILAKFFYNTKNQNLTFQEEFDKLSVKEAASLLNAFEKFFIDSNKNTNTNSTTNNINSKGEQPIEQPEQLPQIKSNNNNDNPIYMIVVFSLIFIILIGILFILRRYLIQQKLATFQMNEEINPQGSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIG0</accession>
    <name>A0A8S1PIG0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1190066</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIG0"/>
    <dbReference type="OMA" id="NTIRMTH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051218">
        <property type="entry name" value="Sec_MonoDiacylglyc_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856:SF25">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035722105" description="Fungal lipase-type domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="86"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8102759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="269" mass="30348" checksum="0757488F52C157BB" modified="2022-10-12" version="1">MRSQPFILIFLATTISATFIYNEDLAKEEAALSFAAYCPTTAINNWKLGYVSANYPNIKNPQVFENIIQGTKGYIAYNPTYNAITIVFRGSSNIQNWLDNIQFDKVTYNDACQCQVHKGFLEAFKSLEPQLDNLLVKYKKMYPNAVLHITGHSLGAAMATLYATQLAIAGNSLQLSTFGLPRVGDKAYYNYFSSFSKVTHFRVVHEKDAVPHVPPQNFGFNHVDREIWYHRASYTVCQLDEDPNCSDSILIPSIADHSFYMGWSSSVDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PK19</accession>
    <name>A0A8S1PK19_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1210074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PK19"/>
    <dbReference type="OMA" id="RMSSWQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="174" mass="21112" checksum="7088143BA68F20F2" modified="2022-10-12" version="1">MNNNNYQFVGMSYTQGSSMNQTQLSHSFARFPLQSSIMSVEEQSPQFAQLAQSQIYIQENSRFQEEENYKHLNQMIQQPQKQLEQQTGHKRMSSWQQQKSPQQQSQSKSLCASPLRQQYRQEEPQMYYHQPYQPCPCELEQLWQEFLQMRMMQRQFRRQTQQCCHHQRGHFMPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PP11</accession>
    <name>A0A8S1PP11_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1250119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PP11"/>
    <dbReference type="OMA" id="HMEHYIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="61" mass="7196" checksum="7B401BCB7978A86B" modified="2022-10-12" version="1">MDFLGIRNNRFIKFKNNLDQDVTFRDEFVLTLDAVSQINVPITQNFKLISTIKHSQKKNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRT7</accession>
    <name>A0A8S1PRT7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1270022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRT7"/>
    <dbReference type="OMA" id="NQWPSIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="337" mass="39187" checksum="C401AD93624EB35D" modified="2022-10-12" version="1">MSNIKFVYSKKVHKIPPNVTNLQNVIEIIKSIYKQLNTVYLYAIINPEETESVAEIRNDIEFQQLKALYKSNQWPSIKLLVTETQDYKSILKESWGLLNQSMVIVEKQSRDVCTLFQPIQIDQSQQVVPLAGHQGTQVQVNQKDNAQNTNQIDFRQNEQLKQYIADIVDKRIRELGLLNKNQNQNYKVTLTSKIPVITGVLGKKMIVQMTILNSGINQWINAFLTNPELGIHQQLNNVSRGECLTVTIDIPYIPEHFENQLERIYKLYVICENEKQQIQKLEDPIEIIVKASQLNQIVEQLQLLFPQKKKEDLVKFVNEKGVNKTFDEFVEMLLQEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PT37</accession>
    <name>A0A8S1PT37_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1310050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PT37"/>
    <dbReference type="OMA" id="TIYHGNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="54230" checksum="C4F907F579536BDD" modified="2022-10-12" version="1">MHDQKCLFQKLEGTLAEKLCIKELLKEQVIIYVQKTKEFIQKEMIISFNALIIDNQYIELSKCQFEERVSKPKNNEGINVIIIKSTAGKEYLITSNSQQCLRIKIYLKKFCVNKNYSGKYRLQEQKNCLPLSQSYKKNNQYFSTIKFEKKQLEENEELQMFHNQLTIMHAFQQIPNVIRVCEDAQRYYILGENMKLQSLESLLLNGFEFKEVPIVSIIFMILQTIQKFQAKTIYHGNINLQNIYVNINSPTLQIASLFPKYKEHNIKNIEKDIYNVGKLLYQITFYTQRGEIPQDINLNLIQSLMDQWSQPNSQRTQYRFLYRVSQLNLLNQLLSPGITVEVALIHQWFVNIKQNLKIEPKQLMSKAFLTTIMEEQEFHMTSSQILDHRVTITQQSYQEYEPDEELYPIRCQMINTTQMIPSKKKHDDAKISLFRSKTFTSSTIKRGSLHEITKQSTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q090</accession>
    <name>A0A8S1Q090_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1380030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q090"/>
    <dbReference type="OMA" id="CKEGYCA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035933822" evidence="1">
        <location>
            <begin position="17"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8108732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="72" mass="8057" checksum="994B99915F751966" modified="2022-10-12" version="1">MKIIIIILLLLYSVQSIWGPTCRTLCYNSKGSICGILSQSCCKEGYCANGWLGEECEIRLFIPGCTTNWSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1N8</accession>
    <name>A0A8S1Q1N8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1400087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1N8"/>
    <dbReference type="OMA" id="IKKYPKP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="311" mass="36420" checksum="F82E0FBE54B8811D" modified="2022-10-12" version="1">MHTSRSAPSLMTIEQMYSVVRGETGAWGIEGYEVPKKYCDPLKQVQEREFLKGNLKKPPTITKRGHFLDDIAKPFRNRKVPVQYNVEYKWVNEKDKERKRSKSELLKRNTFIDDIFLQNSKQNYPKPGPGKYDENRSIENQAKKWNGKSKIQYGEKPDFVQDYQHLGAVLPAGPGSYNPHPILPKLKHNNTNPKIMIAKHKDFDKFKLEMIKKYPKPDCTKYKPFPVDYNTFGRSLELLYDKKKIEPAKIKYWGNPNKPVTRNKSEAKIPPQYNLIAYWPGKEDGSGKNKELKKFNWMNKVTKGIQQSIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2C0</accession>
    <name>A0A8S1Q2C0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">asparaginase</fullName>
            <ecNumber evidence="1">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1390036</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000143">
        <property type="protein sequence ID" value="CAD8109003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2C0"/>
    <dbReference type="OMA" id="FEVQKQH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009066">
        <property type="term" value="P:aspartate family amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="3">
        <location>
            <begin position="42"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="4">
        <location>
            <begin position="277"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="474"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8109003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="649" mass="74111" checksum="FDFAFC3324A23B3C" modified="2022-10-12" version="1">MSSDEDIPKIKPSKSTNYDSMISSIINNEGGQQNLQALNDRKVLLLYASGAAAAEGEVNESHMKIVKGRLEQRLRSISFLCDLEYTQYHNQDGCLSTPLSEFGRRTVYKVMELEQITNSRQTSYADIRHIAEIIKENYEKYSAFVILSGIATMTYLGTNLSFMLENLQKTVVITGSLLPLSFMRNDAFQNILDSLILAGHFLIPEVLIVMDHKAYRANRCRQLKCDSLDCIESPNFPHLVEFGINIDINWQLVLRRGEQMFTNDESTLELAPPFVTDVIIIKITPYTSVEHIKHILNTPNLRCCILECYHFGEMPHSQELYNVLLHAQQQLGIVLIQISQCIKGQPIMNFRKVVNGILVQYDTTPESAQAKIAYLLGKGYSNQEIYQKFPQNLQGETESVRQFEKFEVQKQHFIQTILETLQKTTGDEQISQNMDIMNKYIIPNLGCFLATTGQLELIKDLRKNEGDLSIPDFDGRTILHLAAANKQIEIIKYLVEEVHVEINPIDYLGYSPMYEVLISRDKEMLLYFYQNGGIISAPHHILVDLLLSSGLNGDLQMLELIFHGGIKNLNHFVNIDNRNIAHMAVMSQNTKIIKFLRFKAKFDFSDRDRWGNTPFDNALEIKTKKIKIQQMNDLAIDEIIKILATIGND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5G8</accession>
    <name>A0A8S1Q5G8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1420126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000146">
        <property type="protein sequence ID" value="CAD8110021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5G8"/>
    <dbReference type="OMA" id="SKERSWF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="815"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="123"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="201"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="635"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8110021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="928" mass="110766" checksum="08C46D187591D40F" modified="2022-10-12" version="1">MKSESQSYASYSRNLNKDPQQQSYHSATFQTLQTSQRNYILEQTQNVNDEEKRLLQKRIEDLEFQLKNLSQKPILYEQEIARSTIQRESLQTDFGSANQTIRQLQSKISDLEVSSQIRLSQQNDVSKRQILDLEDRLQKLQIQCDSQVIQINQLQRDKVSLASQCQALQDQIGSSKLNYKQEISEGELRYTKDSQRLSLIVNEKQVNIQQLELRYQQLSDQFQMLQRKYDQDTSSLKVQCNDAEDRYRRLQQQFDQQTILVNTINIEKQNLAVQLDKLQSESNTQIYNYQSTIKTNESKFIRDQQQFQTQLNEKIQQINDLEKKNRQQLDQFFQLQQLYDKDTQALNYRIQDLEDKLNKLSRSFDLQTNELNQIYSDKERIERQYLKLQDEINTQKLRYNQEITKTDELSSKERSWFQVQLNDRQQTITELELKVKQLSEQLQTIQRRTDNDILIFQKQITELQDRLRNQQEQLDLKNNQYIQLNKEKNLILNQFEQLQQEFNDYRRIYENDVSKLNRDNQRITSNVNEKTQSYIQLEGKYQLLNEEFQALQVRFEKETNNYTMRMKELEEDLSIMIENYKREQNINRDWEQQVIRLKQQIKYSVDETRISTEKTLIEKYENLTSEQKQEFDIIFSKLNQDKNELRIQLNQKVDQIRALENQITQLNLQNTKNLTLISELESANQSLKYQVNEQEQKIKTLIMQEFDSLRDKSADQMIRNLMAEIKALQNKLKKYEVYMKNIDEQKLQEQFQNESNLGRSSYVIEQRVTTGSSIIGQGGTSQVNNGKIQQLESSLKISQQRERELQNQINQLSSQLNQQAPNSQRSSFINNQNNDNGQNQLNEKIKNQEREILELKSRIQTTSQTRVDTRYEQQQLDEKNKRIIELENKCALLANENSRLNLMRSKVISAQQNQPYESQSNNGSQRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9H2</accession>
    <name>A0A8S1Q9H2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1500121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="760"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="2998"/>
            <end position="3039"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="3045"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8112272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3045" mass="353877" checksum="F12A263A7962C0E8" modified="2022-10-12" version="1" fragment="single">MTGLLNKVTSPLRGGGCGSLKTIPNTDIVLKSDIQDIENFISRFDSFVQTISTKATVAANQTESQDIMIAIQWFIFQEENIYKLIKNAQKILKSYNLIINGIQKLLESCLIYVKTDSFKCLFILKITASLSKVIFSFHIRNDQRQLSCETKQKFLDICDELKQQMEIEKNDFIQNQLELYLFLTKTSFQMAPNDGKERDDILKGFLNGIFQSMIDMKPNAELLIQLFRGVCMLYNQYVNQKNRQQYEVYFSIDMLQWEIIYNFKNDGQKNLEEILLQIEETHEKIVKNSRNWENHYLWIQMIGTILLYNPLITKKKLQQLTSSFNLEAKSMYIWKEYLNKGFLIKMNHSDDQALILLYQFTNKQLSQIDKLILEDTFKEWENLMVLKDFLLNERNDNIHFTFGSYLQFQLGSMEPQNNEQIQAICKIQQFFLFIISNKLLALIQENDERLEIIVKNYKNFYKENNYFNETIEQATYNQQLKKINISKTLSKLLKLLGIVKGNKLFRQNNLYDEKLVEQILSQAIQDIKNKRNEPDEIIVLQQEIELIQEKFKNIQIQQINNFHFSMSSTQCLLTRIQESEKYVSKIEIVFLQKDNLIQYFQWLLNNLNCNYEEQIQELQKYCQEFIENFKTILILQYEIESVSKTEKLKEIKQKYSSKLSLKCIQQQRILVEGKINQIKEQLNNMGNDIKFLRGKSVVQLLEIRKWKVLKEAAEKNVKSIYVPLKTQERGKNEISNLMNLDQFDDKDGEVNEFLSEEKTVLLIHGLAGSGKSTTAKKIEEFIWKLHDSNKKIGNYVLIPVYISLPSLKNPVFQAVEESLKQDDYGFDVLQLKECKEMLQKQEFRFILIMDSYDEMKLENIQKNLYINNKLKQNWSNPLVIFTTRSDIFTSSNYADWFTPDEKQKFKEIQLLQFEQSQKQEYLKKFTIQSIKMLIFDIHEWQVQTQNQKALDFKRFEQSWEKIQSSFLKFDEARWKSETLLNEQQINSIVQCLKDDELIALKSIEASRNLSINLQKLWSFKNGNSIYDGNYSVSVAQHDSQSNRDYQHQINLLEEFFKDVERILHKQIQNSNVQITTQDQQNNLIQYKGILEYHIFKLTLEEQIIDLEKIDLELIEKEFGELFIEETNPLTLTERITEIIKDFPIEFSLNIINNENLNQNKLNIEREKYEMLLQKLRKIDQQENCEVVEQLKLLICCIEKVVRLLQASVNQNLKFFQIPFNKIVSDLKEKFDEFKKKQKDKNNRHDWNNRERDTFQNKIRKNLSIIVNILQLLLIKIKQVKQMLAEFFEDVKNFTKIFTKSCLYKNITKKCIIAFLVESLCTTANLEQQEKESIKEYFSRIQINPQQSEKQSDNNNSQSTNIVDFLYKLMTETKEKLKISNWEFQKIQFNQDQLIQLTKKIYLQENDEEYYEVESNQQDQFSIDQQKNDKWKIKQGLILTIIQISQNSFTETITRFCQKTLIQLWVLEKDQRVRNLLKNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNKLVETPYMMEIIVQVLPNMILKATEIINIKQTFLKNFSKMLKEFYISKYRIQMYKSQQKRHLQESINEVNEESEAQIQFDNDEFLEVTHNDLENLEIIDYHQIAFEAWNILEENSIPQQLQISQGSEGVHNQLQKIFENNLLPLKNIKIQQEEIIQFANNALKEYNLTIYDFYCEFIKYYHLKQIEKQKNLGKSIDTDRFMHDLLEYSIRFAKIMSKNQLTQVQYKQQGFLYQNERTQDQWLNEFFNYDDQYGAYKKDIRSCSLVQQKGANFQFTHKSIQEFLIAADLYEVLVQSKKFDTQILNIIIEILSKEEIQNQDCLEFLNNINQKYSQYYNQISNLSLFQKQQQLDAFGQTINQITCLIKIIIKHDFHSINYSTEIYTETRQYLIQKIKQEVKIIEFLKFLIHLTKIDKDFIISGSNAINLLVEMQVDLTKQNFENIRIENTNLFGGNFSKCNLSKSLFKNVNINGINLNGAQLFGCNWKELKINDLYQLDGHDDAVWSVCYSPDRNTLASGSSDQSIRLWDVKTGQQKAKLDGHSX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBT1</accession>
    <name>A0A8S1QBT1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1540018</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HNEPIAC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23810">
        <property type="entry name" value="UBCc_BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000002">
        <property type="entry name" value="SUMO-activating enzyme subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2660:FF:000005">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000146">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF4">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="1153"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="2218"/>
            <end position="2258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8113068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2463" mass="287655" checksum="FB303D0A02B30C7A" modified="2022-10-12" version="1">MSYDDYYNYFDIEGCNQQIDIEQLGQFDREQQNSQNGQQFIQILDPNNIEDSGFVFLLQEASTSQLEEAMLQDVSMFFNREAKLFNYLANLFQLTDLDMQGAPEYTNQLTKLLKMFFQIHEIGQHSDFSYQLAQQYGLKDFRTGYPFGFIINSDQSLILLLLKIILSEENLNKENDFFSQIRMYLPRYLIFSINPPKPFVSRTQVRSLQNSIINKINYELFDTNFAKPYKLDLLALSYFSDPGLNFQLFKETIEILRSTLESLLEQKNTQQDINREIQNLLQFFLAFTTNYEILKSFFQINFHKTLYNNLRQCQIIFGCNNPPLWSKEPIIIEQIVSLIVRILSFDQVDKQFIDILHEDFMLLLQHQKLDLIYKVLLPILNSEQVKLVAVCFHPKLSVSESKAETEKFLSNQTKTMAGSTNLVTELLTKNQCDKITQLVQENLNKSIQKQQQQQQNVNKIQIEKQQMELEYNDQAMYDLFSDDFQIEKIQFAPVNLIQQQQIIPVQQNIINENQRQIIGSWIHLHSFSPFNPHFDSQILTKVLDNRTNVLFIFEMMVGQENVKLVCFVNHLIQDGRRQNYVQARSNESSFYLMMVQDEHFWYVNALDGKPHVLLDIQQHMVQISHHNEPIACINFSDFQQSKFGFPFKKLQEKTLLMDQINAALQGLECYLLQIAIYTFDVNKDNLFEDDVQSLKKKYEKQMNYYYSQLRMQTIQFIPNTINGQDLSQLLNENIEIEQLKENSINDIDVIKPVTQQIQIKNKQQKSLLTYFEQKIGGIFMILDYVKKYLDIVQAGQQYKDLIFELTILCQIEGFQEQILQSEGFLQQLLALAYLNKKQECQEIYQKINSLIYSQLVQLIQEKPFLKIVMLLEHKLLEKIVNKTEQQLNLINQNTQPQAQVKFEQPKMQFFKKQTINQGKPKGIGYGDIEYKGFKYINEQQQFNQKQQKEQQEVILNQLDKKQNNQDINLENLLNCLIQLLDLEFINVRVLESIQQSNIMNLLIKYLQNVNITTLEGSKTFNKILQIIECLAQREQTLQLFISKENTCLFRQLESFNQQVIIFIKTFNSKIEELNKFMTLYQYVENCLYAAKLITIKYLSQDQQKDIDVNINMHQFYRPFMKRLAVGKCEIKTDADYLLHMKEYYQQTNNPSQNKMQKLISEITTIEENLPLEATNSIFLRYDNDRMDCMRTIIFGASGTPYAHGAFLYDMFFGDDYPQRPPKMKLATTGHGKVRFNPNLYNCGKVCLSLLGTWGDNWIANFSTILQILVSVQSMVMSEYVMFNEPGWESQMGTPNGEQANRGYCNFIKIQNIRYAMIEQLQNPPRGFEEVIKKSFYLRKDLIMKEIEQWIEQANLPATYNQTQNQCTYTQQPLLYKQDLIKIYEELKVELEKLKFNIGKDFQTISNEKKKEISFNLDNIQPQKQYQIMNKKVMPQGLNINEIDISYNNNVQQRQFDSNDQNLQNLMSRYIGVVGLDAVKKQSESTIFIHILNGLGIEIAKNIVLSGVKRLILFDPQLVQMSDLGSNFYLTDQDINKRRDFSILNKLKHLNPYVKIDVLQNNLDELNLDEIQVFVTQDPTIASIASNQNKHAVVLAQTRNIFVRIITDFGNEFTIVDKDGEQLSEVNIENISNNVVTIFKNQNHNLTENDLVLIQEVKQEQGIGESYNQVFQIKNVKRQSFELVTNKQFTKYISHGVAYQQKQPIKLQFDRIQKVIGSLDHYCDNIGTFDGIDKIKRDIIHFCMNTTSNDQLTNNWDIDKIKMFIISIRQQNLREILNFRYQEDVLYKYQEELLSLLTLLSINTQFQPLCALIGGMAAQEVLKAINKKYTPIHQAYVQSFEDILPFKLKEFNFAQIGLSNNLEININKYQECMQKFGFKQNLNTRYNDLENTIGNTQKIFNAEVFVVGAGAIGCELLKNYAMLGISKSGNIYVTDPDIIENSNLSRQFLFREKHIRKPKSLTAAAVVKQMNPDINIVARLDKVCQETQDIYNNHFYTQMNCVTNALDNVQARLFIDSKCVENKVSLIESGTLGPKGHVQSIIPDVTESYASKQDPEQNNDIPYCTLRMFPESNIHCLEWARDKFEQYFFRKPQALVQLMQDPSPQQQTVDLAIKVLKKYPTTFQQCVHMGRLKFQKLFNHDIMALMNAYPLNSLTKEGKLFWAPPKRPPKPIEFNSEFAFKFVEDFALLTAQIYNIGIPRQYDINKLIQNVQIPKMNIKKNKIQEIVEKQDKNNQLQQMEEEVKNYDQLIKEARQLLSNVKPILPQPQQFEKDDDTNHHVSFITAATNGRAINYGIQQVDWMWTKLKAGRIIPAMATTTSCIAALQTLELIKILQNSTQYRNTFLNLAIPFMMQSEPGEVDKFQLKNGLSISIWTKLNLKIKRLTEPLQYIVKKIEEMIGDEINSLQQGAKVFYMKQMLPKDEEEKYNFINTPIYSCIQFINNSSQINVQINDNKSLVVLITLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QE13</accession>
    <name>A0A8S1QE13_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1570101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000162">
        <property type="protein sequence ID" value="CAD8114008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QE13"/>
    <dbReference type="OMA" id="ICQDVND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018537">
        <property type="entry name" value="Peptidoglycan-bd_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008565">
        <property type="entry name" value="TtsA-like_GH18_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05838">
        <property type="entry name" value="Glyco_hydro_108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09374">
        <property type="entry name" value="PG_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TtsA-like Glycoside hydrolase family 108" evidence="1">
        <location>
            <begin position="12"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidoglycan binding" evidence="2">
        <location>
            <begin position="109"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8114008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="647" mass="75344" checksum="264D895740E35F85" modified="2022-10-12" version="1">MVKDDKFHACLKYIKESEGGDSNHKNDKGGATRQGLSQNAYNLYQKELGNKGQVKSVFELKNEEIEDFYYHQYKKYKVDKIEDINSRYLYYDAVILSGPGRAARMAQSACNIKVDGCFGKQSIASVNSMDQKEFQNKFINIKRNHFHNIVKNKPDQKVFLKGWLNRLDRTLSQIENGTIQKEYDSMQSRKSIQINKSNQQQTQNLNGIDCNVDLSVLNRIKGVNKMSAQFLIESQENIILSDLATVLSVLYDPKLKDEHKSIQFSLDPIILPNQQEFKQQQKVCWPDSYVERNIIQDTEMAKILFDADYLLKLMSLGVEADGKTPFKYPLELQQLGFNSCTSMGKQKQQQMLCRFWLVPQQCFYTSINNKYMIDDIQVACQARQIERVNNKLQDKICQDVNDTAYQFAAKFTQLYDKIAKFYPIFNRLKQLFKAVALGRWMYINNVKTNYQELIRFCKKVNNSPKAIPILKYEKVGEESKTPIYFTQSEKIQVAKDHLIEKGHPTTQDLINQVVKQIPDIKGYQVSTQVQYSQGGVDTHCKNMIESKQDPKINRFNNLEEVDIPFFPQKKCSKCNRMIESHLLNLDQNQCSVHSEYTCYLCLELITNEDLNPKGCIINNYRYIFHQSCYNEYQDIINCKQDNDDDIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QE24</accession>
    <name>A0A8S1QE24_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1570116</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000162">
        <property type="protein sequence ID" value="CAD8114038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QE24"/>
    <dbReference type="OMA" id="QKIDYFF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="81"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="170"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="256"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="657"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8114038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="668" mass="77665" checksum="6A4A3397A5380D09" modified="2022-10-12" version="1">MSKLKRGLTKDFEKEQEEQALAKEELFQPVVQIYQDYQYLSEEQIDVIAKILQVIDFFKENCPDTQTLMEFTKLCCQNLIYEFIPKDSPVFHIDDAGDKFYIVLSGRAGIYIRRQPASIEADEAAAQPRILKMLEKLHLESIQQLQGEELMKFYSQVIKKQAKPQKVIDSELILFRSGNFDKYFTNQGICKFMLVAQKQSGTIFGEMALTNDKPRTASIFGLTDLKLLSLSKANFKQVGEKGMKALQQKIDYFLKMFPHMTKHKMSKLILYFTSAKFPANFFIWKQGDETDGFLLLKDGEMQLLQNVEFPKPPSQNNVDFTLMSLFQNSKQEVKDIKETIILANLTGGCFVGETEVVQKKEKRDYSVKTLTISNCYCLSLENYHIVRKTFPEFFSALNSLQQRNSSLFRKRIEDIVKTKSSNFYLTKKEEALAVERRYANEFGKDEAKPFLSSPILRSTFQSKLSKQQMVEQNKSIIDQHTKLDEQFGEKQEEFNMLKLAGDNFQKCLLIRVEKQFEQFQPAKPKAKTPHFSKQNSTVKDILQSVRRRQFPSFATNRDQLSYRQSNEEDSQQLPQIKVAKQHIRVVNPNISSKVDIIKSNYQSFMKSKSMSSRLDRLSENQMQGFMLTDSINTYHPLHSFQNKDDQTKHKKRIKTQSKVESDYQKSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJZ7</accession>
    <name>A0A8S1QJZ7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TRAF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1670063</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000176">
        <property type="protein sequence ID" value="CAD8116003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJZ7"/>
    <dbReference type="OMA" id="CHADLKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF155">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_56, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAF-type" evidence="5">
        <location>
            <begin position="109"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="4">
        <location>
            <begin position="109"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8116003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="227" mass="26551" checksum="97F15264CBF263F7" modified="2022-10-12" version="1">MNPIDTRQILNWDQVKVHLQPNLCIECQLVPQQPLQCYRCQKLLCQSCHGDLKQKSRGPKIFCQKCKALNYFNPANNAIYQSYRDMVIGCLNRRYGCKVTLDYKKYQKHVEECEFQRIPCPSPGCFVTTLKRAKGDHLLVCEYVIETCQYCNKQFNRKSMAYHQEKCNGKDVQQIDLASLKSDFLRLRSKLDAAISQFEQSTEIAMLEQKVIKQQLEIQQLKNNINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QTX0</accession>
    <name>A0A8S1QTX0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2840001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000293">
        <property type="protein sequence ID" value="CAD8119079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QTX0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8119079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="14775" checksum="9F85444021764719" modified="2022-10-12" version="1">MKYRSLISIKITLTTMMVIQIVNQFTIMIVLSNKYVQSVIMVNAQNFIMNLPLTIIEIYQYVEMDQSKNKKQNAIVQQNLILIIDKLTINFFLVKVIIYIRLVDYNEKIVFVKINWISQVIRINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QUS4</accession>
    <name>A0A8S1QUS4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T2550007</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000264">
        <property type="protein sequence ID" value="CAD8118675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="WYEANEK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="154"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="188"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8118675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="26140" checksum="93F48B1B91FAEDF5" modified="2022-10-12" version="1" fragment="single">MKLHLRIQLLNQKKWYEANEKINQYLKLLERQKSFATIFQSMTLIEMNQPGIGIKMREQAKQINSNLYTDLLDYSNKELTQNPQNKFVLIAKSYALNEEKKYQEAIQQCDKVLIEEPQHLHALYRKSFSLKYLNQNQQAIQCIEKALNFDLKYSIGYMMKGYSLESLGKQKDAIDCYDKAIQLDPNYAKAYINKGISLNNLLKYNDAIDSYDKAIQLDPNYAX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QUU0</accession>
    <name>A0A8S1QUU0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T3460001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000355">
        <property type="protein sequence ID" value="CAD8119586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QUU0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8119586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="61" mass="7051" checksum="C21C1AC1597E403E" modified="2022-10-12" version="1">MFFLLKKNLNDLYFQRFHTTKSKKVCNQMQLIMHANSTGLSTTENNPAGTCNQKVEIVEMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN76</accession>
    <name>A0A8S1JN76_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JN76"/>
    <dbReference type="OMA" id="TYQQQCY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="356" mass="42125" checksum="7C655718351A0D82" modified="2022-10-12" version="1">MNQFPNYLQCQTKRVVPTYQQQCYSKSPQGRKCVVVNQQFSPNEQKYMKQFKCIDQYKYQQPKAIIYPQKRMSPPNKSPLIATKLRIQDKENNNHYYNANIIFQPCKTPRNPQNQQLFSQQNSPNIKQPTPQFENRYDYEDRNQEFRLSTSEVNFRFVDSEKHTSDEQELKNIANQFNKIEIGKKEPEMSPKSIKSDEFLVQMDSVKKSDQLNKSHTKNIGIQAFESIQFEKPEEQLGRIFGSLKKVKIIKKKQTQPTLQTQVTQRTMEDDSMMMKIDTQQDQQAKTISGLSNIDFQSEKLQTEFTDLDSHRTRNAQNDETDQFTNFRQNISTPKQLTQNNNISFLMYKKQFEKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP19</accession>
    <name>A0A8S1JP19_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JP19"/>
    <dbReference type="OMA" id="VCFEDST"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23794" checksum="AE914BA6D599686A" modified="2022-10-12" version="1">MNSKHPVELLKKKIDPEAIMYKFRWSNNSVSIEPMTQLTPEMLVLVHDYELKQLGHDPQEPKPQKHIKQDTQIINQNNNGKKLEEQKREKRSIDKLEKADKTEKIIEKTPEKNSEKQKQQQIQKDVDNVLNNNTGNGPHKPRKSKPCQVKQVRHDNGRVDFLVCFEDQTDQKWISLDEMKEKAPVAVCEFLLGKVKYGGTMNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPN9</accession>
    <name>A0A8S1JPN9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPN9"/>
    <dbReference type="OMA" id="QNTQIDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="85" mass="10048" checksum="574AA3B6EB767C7E" modified="2022-10-12" version="1">MQNLTYSTLNLPSTPQTPSTPTFEQLDQDQSSKHNSRRTLFDLEEEYIIHKNPIFKRRLKSKQNTQIDYTTFLGRNLLENDLESM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRY2</accession>
    <name>A0A8S1JRY2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0060473</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRY2"/>
    <dbReference type="OMA" id="WINIKMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021319">
        <property type="entry name" value="DUF2921"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF185">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RHF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11145">
        <property type="entry name" value="DUF2921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="74"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8044389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="136" mass="16197" checksum="2B11A0AB2B055022" modified="2022-10-12" version="1">MFYQIYNRGCPSNFQGLEPSLLFCMIYLSEYLLQIIVLYIQFKLGPRSFIPKCFLPKQYNYYRVLNIEEDYEECAICLTSLIEDPFDQQVPTDKLILKQAMQTPCHHWFHPSCLRSWINIKMQCPTCRSELPPLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSC5</accession>
    <name>A0A8S1JSC5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0080506</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSC5"/>
    <dbReference type="OMA" id="ITEPIHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="133"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="293" mass="35017" checksum="3BCAAECC504D5564" modified="2022-10-12" version="1">MDSKENYFNIKQMTESFKNVKKYSQHSNIIVDMFIEITQKLDKSSKDYLSKVIPIDDDKQTLNQIDTKIIMNYFKQIVIYLIKTKIELEEKTQQIESQTQYETLIQKLEADVRQHIRIEQQLRIYTETLQQKIDDFVLEREQQTQQIKEYEQQIKQKNKDIVIYQSELRKAVNQNQQRNAKLITEPIHEEYDTKYHLNKLNQTQYTHHRKTSQINDEKSAANKSQINSYKNSPERIPIRKISVTNYAETKTIKQPVFIRQPCPMSYSHLKTEVQLSYRSNSQDSKQASKNIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTM0</accession>
    <name>A0A8S1JTM0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0080518</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTM0"/>
    <dbReference type="OMA" id="SHIYFVM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000210">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000524">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="63"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8044949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="391" mass="44554" checksum="D5AD88680927402E" modified="2022-10-12" version="1">MGNFCPKGDAHSERSSIANIVVEEVEQTKLKATQPMQSSQNLVTSSNVLTVPKPKGYVSRDDFAKIDKLGYGAFGVVFKVRQKTTNKIYAMKQIEKEKIFKNKLQNNTVLERNVLKQSKHPFIVRLKYAFQTNSHIYFVMEYIAGGEFYKILNQVKTGLPENVVKFVAAEVVLALEYLNTKLKVIYRDLKPENLLLTTTGHVKLTDFGLATMRRENGEKSYTVAGTAEYLAPEIVNKSGHSYEVDIWTLGILIYEMINGFTPFRDSNNDFKVISQNIIENQPVFPEKMSQQSVDLIKQILQTNPQERLGVKGQGYDELKRHPFFNGIIWDQLSNLKITSPLKTFAERNTQKMNLVNKQPQNFQNTPCNPQSPKHKIDGITFEGEGTFSSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVN9</accession>
    <name>A0A8S1JVN9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVN9"/>
    <dbReference type="OMA" id="FQQFKVV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="620" mass="71483" checksum="D2974DDC45D8B048" modified="2022-10-12" version="1">MQQSLFFHIRQFFLQFDMFGQPPTFRILGKQKYNSILGAFSTLGTIFFALAYLVVGIQELIAKDSPNVIQSERQVIETSPFYLNRNNFTIAITLADLNSQPLATINKFFTIQLKNCQTTRIYNETSQTSNVTRNCTNIPLEACTQEHFLSETQIDYFSRIRLGSVQCIQKDYWDSHPPVLQGIKTSPIYSSLTISVSVCKNSTNKSDCAPIEDIKKQLNSGFYAVHLSDSLLQMNRAVNMSLDVINIQYYTFSITTSKNIFQQFKVVDTKTDSGVVFSDISDQQVLIQDVARESTDLYNNEYLVYHNINLSSKYSEYQRSYVKIQAILSRVGGLYQMIFLLLAAFIKPLVQLMLDIDMANELFKFNCNNKNKLNISEDNKVIDALAGIVRSRKRLLSPRDTGRLNSENNQEAIINESLNIILKKNNQISRTIKTIIWIFIGCDKEKKKMLDMAKEKYMEKLDVKLIIQKLYEFDQLRDIILTTEQKKLFKYLPKPDIVQDLEQAKCLDQSKQNQQQLQSIAIQEAFEAYEKVLSQGLKSVVNQKLIQSLDPEVQQLFQSLQKSQANQNQFLNEIPLNQSKVRFDVGDLADCQSIYEDCNTNPQRIYTKVPKRMNKYNETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVX7</accession>
    <name>A0A8S1JVX7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVX7"/>
    <dbReference type="OMA" id="YFMISNA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="337" mass="39114" checksum="B09D0F4F41B9060A" modified="2022-10-12" version="1">MKKKQQQASQPQCQTPKSKHVIQPIASPNSNSNSFSQIFQSYYPRTPVRKVNQQKKDQLGQVMEKGVTKYAVVTQVTFNSTTKFKSLSLQEHFAQFQVYDKRNKIADNEWSLLNLKSSSTLSNKTQLFYTLTNVFQEYKKNPNNYFMISNALFLAFFSYSNQKIPYCLFITRQDALNKQLQKSQFLEQKQIESIVLGIETPLSVLFPTNPTQFPTKYTLSLIQRKHILYLFNIILNIGIEKEIMDVAANFTFHNSYFNSVQTYNGVDQIENETQNLFDNNTEYKISFEGLFNIQQIIDLKNQLQQTNPDIVVSDIKLSPLTTLINDKLLDQINNELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXR8</accession>
    <name>A0A8S1JXR8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXR8"/>
    <dbReference type="OMA" id="HDVYSAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="113" mass="13575" checksum="492D45B14A48A044" modified="2022-10-12" version="1">MQRKQQYESDFNNFQEASKISLDQMSKQQDLNKEACLKNAKTFELFATCMERHEGKIDNIAKYLSMKMLFIEKQALECYKGNTNDREYERCHQRVEQQLHDVYSQYYQGLLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXS8</accession>
    <name>A0A8S1JXS8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXS8"/>
    <dbReference type="OMA" id="APTQNEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="129" mass="15165" checksum="575262C95E8A9CA5" modified="2022-10-12" version="1">MNKKYSHLQGWEDYSVSNTPTNQLKFLSESLNYVNTAPTQNEYKLHQSVSPKKRRIIQCYIKNSVVIDNGYVLSQKQHYLQGNFIKKAQFSPKKQLKHSYIGPILQQIKIYEKNKQIGKNDSIIKQFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZ16</accession>
    <name>A0A8S1JZ16_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0100485</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the amino acid/polyamine transporter 2 family. SLC38A9 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FYALIMG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF244">
        <property type="entry name" value="NEUTRAL AMINO ACID TRANSPORTER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="262"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="16">
        <location>
            <begin position="29"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8046589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="617" mass="71621" checksum="741F20B3118ABFB5" modified="2022-10-12" version="1">MEEVRFKHRKASKSSSVLSMLAIQRPDKTQSSLQTIVGIVNSMVGSLCLVLPLVFLNYGVISCTVIMIILGFVQYNTCSLLILHLKDQEIDTEHMIKRILGKPWMQAFRFCSGTLLFIVGIIYFQLINLTLYPITTYILSYNDIEFADASENFVFNKYSIQWQALIVFLPLSTLLLIKDITTIVKIAHYGVIALFAYGIFIIYIFIVNLTSEDISQKFQQITLWSWEFSQPAGQFALAFMIHNAIGQLMKNNEKRDNNSRDLAIAYTIAGIIYGIVGIFGSIGILGIENVHDAQTILNCFKKSDIEIIIIESLFLVHLVTAFPIFNNISKYQILEVLYNKKEPSKMVYWGFSILFMVLCLTIQILNIPVGVVISFDGAVCGFLLVYIVPISLHLKCYYTQNKVTLIGDEDDQFNTQQNCVNHKDKNSIPMFARIAFYTLMMGIGIYNLFRQLVCVPSFNYKLRRPAPKMSEYDKERIEYKHLMNQYRSRIVKDFWEEQTKIENQYIDDYLAKEKAKQERLDIYRRNQTVIQTFNATQQFNKLQDIRRVRNERVKKYLVEQDIKKMNRQQIIRVLNEESKNWISDKNSYEKLVGNLLIPDTVLDEMSYYXLVNLLQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K123</accession>
    <name>A0A8S1K123_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0130293</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the metallo-beta-lactamase superfamily. cNMP phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K123"/>
    <dbReference type="OMA" id="YDCPEQD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000312">
        <property type="entry name" value="cGMP-dependent 3',5'-cGMP phosphodiesterase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000029">
        <property type="entry name" value="Cyclic nucleotide-binding domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000283">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="10">
        <location>
            <begin position="616"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="10">
        <location>
            <begin position="752"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8049180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="869" mass="99068" checksum="DA600C7C732B8889" modified="2022-10-12" version="1">MACSYVFSDDKSFLQDLDFMLEQSDQCPSLLKYQSCSIQLQKQQSDTKSNVSCADLVDLERGGCILQTKIGPIQYGMPPETIKDCLNLGMEVPTFYIIPTKRFDKRLGISTAEFEFPAYYNFFCKKKQITLICTPDTEKAIRTVFQETLLGPIDHPDLALDFYHKFPKSAYPDFHKERSVFSRNPSNLDEKLTIDHLLKFCLFDENNTATINEGDITIKIIKDSNQFKILENGIYQKSIQDTFTFPSQYYEYIVTKPDDYRAESMKNIKSKRDAAKEQCPTPLLVKQLPEMTVEEKVENWVIGNKYQRGGEKKLSVVLWDQMGGHVESGSAIFQLDIDENKSFTPPDFGVTILGCSHGFDPKGSTSGYIFWINGRGIMVDPPPFASYHMKQMGIPSILICAVIISHCHADHDAGTFHKILDDTRVEIITTRTIMNSFLRKYSAMSNIDIDSLRRLFIFRPAIIGIPLNIYGAYFNFFYAMHTIPSLGFSVKLENKSIYFSSDTFFDPEQLLVYKNQGILSQKRYEHLAFIEFKEDLILHEAGVPPIHTAQSVLGKLPNNIKNRLYLVHTAQKDLKKELGLKIAKTGIENTMILIPSNTNKHLTTIRRLDLLSTIDIFEHLTLKNVRWLLDSVTAEEYFPGQDIIKEGTSGDRFYIIESGLARVYSKQKGQEFERYYQVGDYFGESAVLSNEGKRGATVEAVTNLKVLILEKHDFWFIFGDGLGGQGPIIQKMKQLTAARRSKAVHCLFKNSILSELTPSQKTQLEMIMREHEIEKGTVLWKVGEKPEFAFIIKRGTFAFYDSPEQDLEELDSGAYVGELKAMIENTAISTSIRATRKATIFKIAKKDLISFANKHPGIYMILIDTKYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3H5</accession>
    <name>A0A8S1K3H5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MtA protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3H5"/>
    <dbReference type="OMA" id="PIDIMTD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1037"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1068"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1099"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1180"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1209"/>
            <end position="1229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1277" mass="149290" checksum="45C915B633F36D63" modified="2022-10-12" version="1">MLALLLLIIFQVQTQTIQSKAIVKEFPSDEDLFSITFQITSTEKINTISIDLKELKCLQTSTITPIFIKKNEPNPYQFTMNFESNTISIYLDFGQMVYSQLEVLFEVTQFYYPTFNQNLNYQVRVNNQDIRTFNVVRQQRVLDQLQISSGSSITQQYTDLNIEFINTYKNYLYDVIVIKFYPTYVFDIEISSCIGIQNIGKDLNCWTKDTNIYIQQKTEDFVQKSYQNPIKIKLIDFLNPYYVGIINMEVLIQPIQFIDTSIVSSHDSIYYQVKKQKNQQIIITQQNNYINSISQIDLIIHTQRFNQYLQLEIYLPNKSSQDLIIKSNDNNMQVENLMSVNKSVQLLKLIINEPKTNYDIKLIVENYQNIDVPCQEKAFYFLTEIFDFSDDQLQIQQGKGVLNFIPNNIQLISIETTNNYITQSAKTIIKFGIDITIKQDSLLLIIFNENLNISKISTLNQIINDNTVSLYGIFNEQKSEYTIEFENIINPIVPCKFNYLDLQIIYLNQAFASNQNQIEIEIIPEIITMNNLNLGYLITNTYTNLNLDINFNLQHQYFTNIEFTFDESNINFNQLTKQIECYFDAVSYICQIHQNKLTIFGLRLIKTQAHLEILHFPYPRSLQSLIIKSVRQLNSSIILSETADVYQIQPIYQREFNKFQISLQSVIKKQQIIPMNIIIIPSQDILNQEYLIFKFDNYLTIESDSQCQINNDIVNIQCEFTDGQIKILIKSGTLIHSMSYSILINKIKVIRDYQTLQLSAFTQNNYLQQQSFYQYKIKQQSQSTEFVDFTFDPLNIDFSSQSNYNLFFENAINIPINGYLQLSSTQLSIDFLQGQVIQVSGEEDISYTKIIKQNQKYSLQFYFNKEVYSGMHLFKMQNISNPLYQQRKEYYTFELQSYDQYNNLIEFKEYDLYSIFICPQSKCQICNQQEFCDKCEADYILYQGQCLKQCPTKTVLIDNKCQRCNAGSSCLQCSDIDTVLCLRCNDGYDLINGFCIWTTILNSTQNVNSSNNTSNDNIHNDTTILTNKTIQQQQQQIYDIHIFAIIVLSFAIIFILRNIFRKQTDFNNALLAFTGISDFVLQFILFVIIMINYTISQSIISLTLCFVTIIQQLYAYQTIKLPLQIDIVYQKCQDSSRIAKIIKFFVLFIDWKTIFIHKSGITNSQLFSIHFQGEIKLKRLFNFLFLVKIVINITNLAMLGVILISQNQIAQLIYESIFYNLAMIIIQLNKIYEIKNSLKNDIPIDIMTDKLNQDNENQNQFKNTSKVTPETVIISQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4N2</accession>
    <name>A0A8S1K4N2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4N2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="11336" checksum="7B34F17D1FAF36B1" modified="2022-10-12" version="1">MQFFVLHVNNLFHNLQIWHQNSIARVAYDQQQDLDKYAHQQFKISCFLTFIFLSIASLFNLLDKEVRILYFNAFLVSQWVQQKFQYKINLIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K5M7</accession>
    <name>A0A8S1K5M7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0170128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K5M7"/>
    <dbReference type="OMA" id="VQNTENF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05992">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="204"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="258"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8050579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="362" mass="43111" checksum="1B38F481CF51B769" modified="2022-10-12" version="1">MNIIAKYKEFEKTLENIVTYKDLRKSIRQSFKFILKNNFTIYYLDEDNDKITISNQEDLDVIQNTENFEIIVEEIDDASELTDDSFQKLDKSVKETVNYFRKLSNGHANNDSNSPKIILQQEMEQAKEIIETTNNTQEILDKKRQRVIQEITTTIDHIRSEILKIENDDTEKRLNEKLEKLQKPQINPSSLSKELSELPIIQQIEQAENNIINLNKVEREELKRNYNNLVKQVTNIISSRQKNQATQIALTKEVIKTEQKLKLQITKTNKKNQRLLKQYNGLLSQYQKKLKEAENKKYNFEKIEYMNKEQLENFLDKQMKEELKAKQEKIEKLKQKEEIINIENQKNEQEKLNECDQFEDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAF3</accession>
    <name>A0A8S1KAF3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">NFACT RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0170156</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NEMF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAF3"/>
    <dbReference type="OMA" id="MFLEFFA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.310.10:FF:000001">
        <property type="entry name" value="Nuclear export mediator factor Nemf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021846">
        <property type="entry name" value="NFACT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11923">
        <property type="entry name" value="NFACT-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05833">
        <property type="entry name" value="NFACT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="9">
        <location>
            <begin position="483"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="NFACT protein C-terminal" evidence="10">
        <location>
            <begin position="958"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="624"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="705"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="437"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="624"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="634"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="654"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="706"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="718"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="764"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="794"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="805"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="840"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="858"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="872"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="888"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="909"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="924"/>
            <end position="937"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8050635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1070" mass="125966" checksum="BABAB26BA62DCDA3" modified="2022-10-12" version="1">MMKNKIRLTALDIMALVTELKQKLIGTRLSNIYNIDAKTYVFKFSLQESKSYLVIENGLRFNLSDTIEKNKVPSGFTMKFRKFLRSRRLESIEQIGVERVVVFTFGREDHTYYLILELYSQGNIILADKDYRIIQLTRQHEFSENAKVAPNEIYPFEFTATNYLEKFDTSMERIQKVISEKAGQKLKEVVFKLVPCLHQTLTDDIIQQLQMNQNEKIVNQFDNVKKVVDYAMEYINKYRSQTQYKGYLCAKEAPKDAEQKPKFFDFAADQPGYYQGKYVVETPTFNEAVHQYFLVVDRQEENKQSIEDIAWKKFENIKQDQMSRIQKLQVEQDEYIMKAGLIQENINDVQAIIDIIQKMIDNGIPWDKIQRMINDSKKEGNPLSNMIGGMNLKQNKVIILLGNKEDEYSDLVQIEIDITQSAYQNARKYYESKKKNRDKEIKTKEAVEQALKQAEKTALKEIEREKNKIQKVQNQRKKYWFEKFYWFISSDGYLVISGKDVQQNEMIVKRYMNKDDIYMHADIYGSASTIVKNPNEVPIPETTIMQAATATICRSKSWDAKIVVSAWWVHASQVSKSAPTGMNIPAGSFMIYGKKNFIYPPRLEMGCTILYQLDQDSIKRHEEERKKKLREEQSQVDETEQNESEITEKNILDSDNDDNDDNNNEDDNKVDLVDLEKTNSVQTIQQGDKEYTIVEVNPNARMIQQRNDKKQKEKDQQQKQQQQQQQQQQQSKQQQQQQQQSKQQQQQQQSKQQPKQQQQQPKQQEQKQNAKDAENDSSEDDKKNQKNPKTQQVRGKKNKMKKVKEKYADQSDEERELRQKLMGATYMKNEVRPEKKKKNQDKNESTKQDQDQPQQQEEQIKDKEYYKKQQLKQDSITNKLLKQQGIEVTKDEDKPDQQVQEVHQEKIEQQLQQNQQQLQQEVLQQKEPENLDNKQEEVDSDDEKQEKQPEQEEENIEYTEMQKLVSYLYADDKYLSLIPMVAPYSVIGTYKFKIKIAPGSLKKGKAGKEILNFFQVNKDITNQERQLLKMITDEEIVQTMLPGVKLTGVGMLQMKQKEKQLKKQQPKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KBU8</accession>
    <name>A0A8S1KBU8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KBU8"/>
    <dbReference type="OMA" id="YPIKVHR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="168" mass="19826" checksum="324F7EDBC634A3B3" modified="2022-10-12" version="1">MGSSCQKQNEIITAYQESEAIEFDDQRIAKEIYPIKVHRGFRNTFQKNKQVNKNVEATSLTLLQQELSKYTKQNYCTEKIIPTQIKQEHKKKNKNKKQLKGIIKQHTSCQLIQSSTRLNYHLKNQEYEFRVGQRIHSEKQVRFNIPKLNNNSSRSCSNQKSRSKKSHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDC2</accession>
    <name>A0A8S1KDC2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitinyl hydrolase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0200234</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDC2"/>
    <dbReference type="OMA" id="INDAWKC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000150">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF46">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUSP" evidence="3">
        <location>
            <begin position="83"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="2">
        <location>
            <begin position="268"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="48"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8053249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="577" mass="67403" checksum="6ED07711753540B4" modified="2022-10-12" version="1">MFICCNTKPKQDKSQKNTSTTAYSDVESIKQSKCEEQIHKEPVQDTANKQLIMELNQKDKKLQEIQNIVNRIKLYREEGKLNLSIDIEKSFVTSLIEEDIPTLYFVEYKWAVNYTTFLERRIQEVPKKINNTKLLTEDNTQKELIIIKSQKDSQLLPGLQPDKHYVLLNERSWTFIHLLYGGGPKIIIDLEKPVTSPDIINDGQTNVTSNTANTTKLSIEPLIDIAKLEYNNLDDSDKSPNTNTTRRPYPQKTNIIKIEPKPYELPYVGLQNPKYYCYMNSALQCLLSIRELNDSLLKQQKQSNKKFTMAYQDLLRLVQSSIPGSAISVEKLQNMCLNKFKYSQQQDAHEFLLYLLSEIQQELVGKNKYQKEEFQNALEAWDVYKTRNPDIITDLFAGQIASKSHCLKCKEISEGFDPILDLNLPLSKYYIPREFKLQDCLQNYFKEEQINDAWKCDKCQFINKSVLRKIQITQTPKYLILHLKRFTQFPKSQKITDEVTYPEILDIKQFCAENVEYTKYTLKGVISHMGQLNGGHYVAYAQRQNTWYHFDDNIVTKDKNNKHLSDKGAYIIFYEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KF97</accession>
    <name>A0A8S1KF97_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KF97"/>
    <dbReference type="OMA" id="HYSQIVE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="392" mass="47636" checksum="C9BDDBDCC1FC648E" modified="2022-10-12" version="1">MKSLKIKTHQYFKKSEIDKNQLIATLPYCVKIVSSGEDEMNDIIKLEFDRVNCIPLNEIITYIDLLLFRFTHGQIFSLIHKLLDLIEKLKYQNINQYHIWLIFNQQHDQRLELSKRSFLDYQLFYSFPLQKQDEQDKSQIIQLITKLLQLCNDIPSCYYFIESNKPYKIYEKELKQEFQQFQQKFNDYYKNPKNIDQQNQIDLNIDTKDDFVQNQNDYQKLEMFVSEYIKQNYNLSPLDTILNQLLKYYQFKQGLISLGMHADKHKKNKKYELKHLNCIFKLKLEQQLNQLISQMFQSLNEQQEILRDSIKYEDYPLSQEELQNLYESAAITYFKHQGYFNSFDQFIEITQQIIDKQFNFEDKFQYILKPFIQEHYSQIVEIKILELINELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKX7</accession>
    <name>A0A8S1KKX7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Chromosome segregation in meiosis protein 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0180232</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Plays an important role in the control of DNA replication and the maintenance of replication fork stability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CSM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKX7"/>
    <dbReference type="OMA" id="QPINDQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012923">
        <property type="entry name" value="Csm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040038">
        <property type="entry name" value="TIPIN/Csm3/Swi3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13220">
        <property type="entry name" value="TIMELESS INTERACTING-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13220:SF11">
        <property type="entry name" value="TIMELESS-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07962">
        <property type="entry name" value="Swi3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromosome segregation in meiosis protein 3" evidence="8">
        <location>
            <begin position="50"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8051614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="266" mass="31739" checksum="84D2EF6D1F969472" modified="2022-10-12" version="1">MSYNQEENEDLGLENRSPRNEEEEDKKDQEQDAQNQLNKKKKKKTQQFRLKPEQFTDPQKGVIYLYNLCKDYRFGNEELEELNKYMHVIEEWHYQVMPKYDFDYFTNRLQKFGGNNSVQTHLQFLRKAHKGLIPWHFVLNPLGEQPINDQTINQTFNETINNNSAIQEEQPQLNIQQIDQNLSQPDLQQNITSTISQKKPLKLSLKKQNLQSQVKSVKKELTVRFNDEVQYEPTPKPIIQEDENYDDIFEQLEAAYKIKEEKKLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KMA8</accession>
    <name>A0A8S1KMA8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP4R2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KMA8"/>
    <dbReference type="OMA" id="NWLYLRS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030289">
        <property type="term" value="C:protein phosphatase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015267">
        <property type="entry name" value="PPP4R2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16487">
        <property type="entry name" value="PPP4R2-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16487:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09184">
        <property type="entry name" value="PPP4R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="156" mass="18492" checksum="F4F0F822692495FC" modified="2022-10-12" version="1">MNENKEMIQHDYQTQLKDFYEQTQEIVSSIQQGIRIKKDIPIQINNLMGQLKPILESISQNGEIQKYNWLHLRSLIVITTRDMLLNMQKLYPYEKTAQNEGFEDELDIVLQFLCVFEQKPPFTLQRICELLIDPQKHYKSSKKILFAMEKLVNVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQI6</accession>
    <name>A0A8S1KQI6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0260069</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="963"/>
            <end position="1018"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8057488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1047" mass="120961" checksum="26A374F4F8FC6244" modified="2022-10-12" version="1">MSSLESSPRNSLNIVDILEKYKQKKAIMSPLPLESFQQIVQEAGQELLQQHPEGDNSHFISFKQLNQDAIDELQRSLHQSKIMWNNFSRALFAASPIEKKNSSTKMELLHNRDAYYRIKRQNEIQLKLQLDGINKEYQRIHIETQGVKSYPSISTKDQFRQQQIKRQLQMGCCQELVRQETEFIKQQIELTKSRLINEIKKIVVYIYTNQNNGWNPSSRESSAMVNYNEQLYLYGGSGAGYISDLCQATFDKQFYKWTMIKLKQLIQCRSYHTANLYKSQLIIFGGIQNSPLKEDRIHCEVTNEVTHINLVNYEIKNVLHAGIVSPRKAHIAEVVGKYLIVQGGIDQKGQYLNDFLAYDIVTLRWQNIEINAPIFQDGIAFHKSCCTLEHKSIDIYKHDPDMTYENQGIYIFGGLDSNGHYLDKLIKIDVRRRPLFIEEVQSKGKTPISRCQHSMTYVELSQQIVIYGGKNDDINTQGFLNDLHILEIRNLSWTYVEIRGHPISGRCSHSAAVIDDRLFIFGGVNYTGFVKSDLLIIELNXKRQNEIQLKLQLDGINKEYQRIHIETQGVKSYPSISTKDQFRQQQIKRQLQMGCCQELVRQETEFIKQQIELTKSRLINEIKKIVVYIYTNQNNGWNPSSRESSAMVNYNEQLYLYGGSGAGYISDLCQATFDKQFYKWTMIKLKQLIQCRSYHTANLYKSQLIIFGGIQNSPLKEDRIHCEVTNEVTHINLVNYEIKNVLHAGIVSPRKAHIAEVVGKYLIVQGGIDQKGQYLNDFLAYDIVTLRWQNIEINAPIFQDGIAFHKSCCTLEHKSIDIYKHDPDMTYENQGIYIFGGLDSNGHYLDKLIKIDVRRRPLFIEEVQSKGKTPISRCQHSMTYVELSQQIVIYGGKNDDINTQGFLNDLHILEIRNLSWTYVEIRGHPISGRCSHSAAVIDDRLFIFGGVNYTGFVKSDLLIIELNQSKAQELSQYEAQTERLSKQQTPVQYKPATPAKIVLNLKQQIEQIKKDSEKVKLADKLKKQSMARHSRNLTDIQIYKTFATVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQY4</accession>
    <name>A0A8S1KQY4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cyclin-dependent kinase 2 homolog</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cell division control protein 2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">cdc2-related kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0260154</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">May form a complex composed of at least the catalytic subunit CRK2 and a cyclin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQY4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001126">
        <property type="entry name" value="Cell Division Cycle related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF254">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="10"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8057658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="272" mass="31429" checksum="CA5C1AFA4AFD52D8" modified="2022-10-12" version="1">MQDNKKVQQFILQEKVGFGAFGEVYKAIDENTSEIFAIKRIFSGRSQQIDQSTIREIAALSSLKGQNHIVPLQKVIFEKGCVYIVLPFYPYNLYEHIKQNDDINYKKVFKQLLLGVYNIHKLGFMHRDLKPLNILVDDNQNVYISDFGIARMNFGQCRNFNSNCEKHSYEVITLHFRPPENLLGCDNYHVSVDVWSLGCVFYQVVMKEILFYGDSQIEMLFKIFSKLGTPSAFIAPSLCSLPLFNEAAAFPRFYPTNYSLGLDMKELIQQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRX9</accession>
    <name>A0A8S1KRX9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0240097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRX9"/>
    <dbReference type="OMA" id="QIDFFEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006789">
        <property type="entry name" value="ARPC5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04699">
        <property type="entry name" value="P16-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8056012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="149" mass="17011" checksum="3E0BD7D4755215A8" modified="2022-10-12" version="1">MEQEKTKKTQKDYDMEAAEELKQLKLPGDLVGNVAATLLKSVQKVQNEDIKLLALSKFHQTLCKFTVQNVQGELLQKLPNDKLIIFFDYVNYSFECLSKGMKIPETSNSYNATFLLNVYNKLVELRGLPIIIESISQKKTLPTQIITIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTJ0</accession>
    <name>A0A8S1KTJ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210379</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTJ0"/>
    <dbReference type="OMA" id="DYADQEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006818">
        <property type="entry name" value="ASF1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040">
        <property type="entry name" value="ANTI-SILENCING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040:SF0">
        <property type="entry name" value="HISTONE CHAPERONE ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04729">
        <property type="entry name" value="ASF1_hist_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="207" mass="24034" checksum="61BCCC69F2D68EDF" modified="2022-10-12" version="1">MALVEVTNIIFENELALFQTPISMQITFEVLNDLTDEIEWNLIYIGSPLSEKFDQVLDNFSMGPLQRGVMQFTITSQPPNYQLIPSKEDLFSVSALILTAKYRQKEFFRVGYYVYNNYTEAELIENEPQVVLIDRVYRQIMGSNPRITKFPIDWEGQLTQLYVPPTNQQFMFESLIQQQQGGVGMQTEQNEQVQQGQQSSTFTQNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUH8</accession>
    <name>A0A8S1KUH8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0270245</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUH8"/>
    <dbReference type="OMA" id="KRTSMMD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000244">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF86">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="757"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8058487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="999" mass="114506" checksum="255FB366291BC95F" modified="2022-10-12" version="1">MAEHEHEFEGHEEELEAPKDSLWRDEHEMEKSANIVYFLTLGIVSGLILREINKKTKFPYSPMILSLGILLGLLQKSLGYVGESASILSKMHPHLIVFVFVPVLLFESAFNCDWYTFKYQMVNILLLAGPGCGWGAILLGAVFKMVLRYDDHDMTWYQAFTLGSVLSATDPVAVVALLKELGASLAFNHLIEGEALLNDGVAMVFFIFFNKFSKAASGKGEAVTASQVVLNFVRNSLVGPALGLILGILAALWTKRILGDDIEVTWLTFVFTYLTFYWAEFCFFKTSGLLAVVGLGLFWSAFGKTRIRSSVEHSVHTVWGFVQYSCDTLIFLLVGIIVGTQVIEETFIHKSDYIRMIVFYFFMILARFIMILTFWPFLRCFGYPISKSEFIVLVYGGLRGALGLTLSLMVGCDEELPARFRHLSVFYGAGMAAITNLINGTTCKALVQYLEMIENPVVKKKVYKKYLEELIVNSQDKMRELESDQFYSMADWNKVNQLIGQPKFIEKIEKLETEIKYMIGSNKVSTNSSNLYEGLTDQEIFGEVRYRIYRIMKGLYYDKFEYGLCEEDTVRLLVESSDIGLDHTKAILNIWDQLYKNFLNFSSVNFFFKVKEMPFIGPFARDYMIKHLGFVYDVTTTFLSCANEAFHLTESFPMSKDAVRVVMEELNKEIEKAEGYLSILNDTFPEIVRAIQTKRASHSILTHQRHYLDDTQQNGLVDDKEYQLLKKEINTRLVDLENHQFDMILPSFHVLAMEFPIFSGLVSTDLDNIIKSAYEKKFGPDEIIYEQGMTCQNIYIVSKGNVVDEFQGGSIKKGLGSLITYTNLIGDGTCMSTAKTTADSLLYSLNLKILKDLMNKNPDFEFKIYINSVEYLRKMFENQAGPLAHLEIKRLLDFFRTKSKLRKFTTNQKCDFIFGGYLFRGELKDINNKVYSQYTYIPPQDTECLVTKDCQCLIFEDSVESFNNQMLKQLDLDPHKDQQIQERYSQIKRTSMMDKSKHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2X8</accession>
    <name>A0A8S1L2X8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0300199</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2X8"/>
    <dbReference type="OMA" id="TTILCYR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003923">
        <property type="term" value="F:GPI-anchor transamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000052">
        <property type="entry name" value="GPI8 transamidase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028361">
        <property type="entry name" value="GPI_transamidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001096">
        <property type="entry name" value="Peptidase_C13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067:SF1">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01650">
        <property type="entry name" value="Peptidase_C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8060565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="309" mass="36387" checksum="8809E7AB8410A261" modified="2022-10-12" version="1">MIIFILFTTILCYRPIPQNQYIILSTSKFWFNYRQAINSLLIYQQLKDWNINDDQISLMIPEDTACNRKNNVPGVACAYDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQSRRLTLSKEQKLLMFMNGHGGDGYTKMQDTTYLLDFEMEKITKEMEFLEIYQEAFMISDSCGAITLFETVKAKNMILLGSSSLGEKAYSHGRCSILSIPKTDKFSLTTHYWLKNELDKKPNLTLLDQMKKYDYAYHKANSKLIINIENKEAKDIKAADYWKPKQPILNQIILKNDQDILAQQEFLELMKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L8M5</accession>
    <name>A0A8S1L8M5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0350195</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L8M5"/>
    <dbReference type="OMA" id="NDYISVG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025:SF3">
        <property type="entry name" value="HORMONE-SENSITIVE LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="352"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8063897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="600" mass="69872" checksum="B8FC1D30E4E2FBE6" modified="2022-10-12" version="1">MKKVQHSEGMIDAIYYLQKCQLIMKHFNTQNPDVIEWLFQLDETQKQFEQNQCHHPLLEQCAQFINHKIIHPFLLCLEQVLQFKNFEQELQKHKLEIDYVIRRLQIIREFMPNISKVPLDDIYCLSNTHQEWAHLAKYIQFIDLDDPEILDLQFEKFNDYISVGCAYAKLGNMIKLPVFSMIFQNLGGAFLITNIEKARQIKLKEMTNPSVKFIQNLWSIQDKAPVLKNFMKYSMTKIQSHHKIYVPYLFKPTFTLERINQEIESGTLFQQEEFKGFDLIDENLYEKLIQSLFRKDRQNKIKVRVLSANKEVFRSKIDEELIVKQIANISKDHNNIFEGTSPGLDGKIKKVILQIHGGGWVGMSSFSHQTYTRKWANHIGEDCVIFSIDYRLAPKYPYPYALEDVWQLYLWILYFSKFYMNINLEQLILAGDSAGGNMALGICFRAIKMGIKVPDGLFLPYPVVNLNFKVFNPYLLNGLIDQIAPTTILVVVLREYLNSGKEHPDTDPYLSPIIADDSFLTKLPKMRIICGAKDSLTGDTIRFVNKMRKLHHDIKLDIFNCLSHGFMNFEVPIVGVTHIGSVINISCKMVQELFKSSEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LAJ2</accession>
    <name>A0A8S1LAJ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0290317</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8060024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LAJ2"/>
    <dbReference type="OMA" id="NQLICQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011124">
        <property type="entry name" value="Znf_CW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34451">
        <property type="entry name" value="PHD FINGER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34451:SF7">
        <property type="entry name" value="PHD FINGER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51050">
        <property type="entry name" value="ZF_CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="132"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="CW-type" evidence="7">
        <location>
            <begin position="148"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="287"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="291"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8060024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="39526" checksum="EFD1A388C53B08FF" modified="2022-10-12" version="1">MKSLLFPGLFQQVQDNTNRTLQPQDLFNNNFGFGSAINPLDYTKLLQKSLQMTNPSSLNPFMSNQLICQMGLGNQKKSNTYNNPITVEDDEPQQVQKCHNQKCNNKGDRKAKSRKGEALQFCEKCLRLYNRGNYCDFCEQVYSNGANDQDEQEWIQCDSCEKWNHLNCEAKFRNQNIKEETENKVYYCLNCTKTNKKQQQTSQPQKKQEKSVEEYLPKQRQIIECDDNRMREKNINFVATRDNKVLFTFRFNLYEDEIKQDLDNLRNAGKKMIKKQNIVDSPIMKVNSTPQQQQQQQQQQQQQQEDKNSEFQVNSRLRTRPNNKNKVNYRYLGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LGA6</accession>
    <name>A0A8S1LGA6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0390266</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LGA6"/>
    <dbReference type="OMA" id="IAYINEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08215">
        <property type="entry name" value="STKc_Nek"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001540">
        <property type="entry name" value="CAMK family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000631">
        <property type="entry name" value="Serine/threonine-protein kinase NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="4"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="360"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="394"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8066727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="687" mass="80954" checksum="43B419A265EC53F1" modified="2022-10-12" version="1">MEFYEIVKSIGSGSFGQVYLARNKREDRLYVIKRIKIRDMSQKDRENTENEVRLLQKLRHPNIVAYKDSYLDREQYLNIVMIHCEGGDIYQKIRNKKSFPESQILDWFAQMTLALCYLHEQKILHRDMKTQNIFLKNGRVRLGDFGIAKVLDSTRDLANTCIGTPYYMSPELFKYKPYSYKSDVWALGCCLYEMCNLRHAFDAQSMNGLALKILKGSYSSISPTYSKALRDLINKMLNTNPKARPTIQEIVHKPIIKLRIIYYMLEVFSEPQSADLDDMYVDTLYEQAEQIGVLPLIQHYQKQIAQGATIADIKNELEKGGRAEQRMLIQIQEQEALKLKQLKKEQIEKKKLEEEIKRLEQKKQQKVPIQNTKQNETNSNSKATTEIITNKSENTQKTDTQQRPDYHRVNQRKVSLERDRRPNEIRQKQKERSLSLEKDRLRDDSTISAKERVIQKKQKRDEEKIIQEEKERQQIYKESYQNRKIAQERKQAQYRQSKDSSLKVQEEFYDSEDSDGVNAIQEIENEEEYEEEGDVDQKLDLMKTKLKDKTLKIQEISQSLQQNRQVQNMQIKALETGQVIPLVEEELENEDLDEEDQGDNDGIDQDEIKPINSIVARIQDRIKLLKHRCEGGLGNNLVEKALQLMKSKQFSTQDLRKQLMQLLGEENIGYWQLFDQILYMEELLTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LK19</accession>
    <name>A0A8S1LK19_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-related protein Rab-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Small GTP-binding protein rab1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0390285</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Protein transport. Probably involved in vesicular traffic from ER to Golgi.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LK19"/>
    <dbReference type="OMA" id="MSRQGNS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001018">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8066765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="208" mass="23441" checksum="2E5481DB6E3E4706" modified="2022-10-12" version="1">MSRQGNSQDYDWLVKVIVIGDSGVGKTNILSQFCDAKFSITHMATLGVDFKIKTIDVDGKKLKLQIWDTAGQERFRNITKTYYKGAQGVILTYSVIDRQSFQNVDGWLRSIQENTNSSDVQLVLVGNKADMSAERQVTLEEGNKLSQQYNIPFFETSAKSGMNINEAFQNLAQRIIQTLSKMQANDEINNLNLKPLQPKEKSKTESCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LP43</accession>
    <name>A0A8S1LP43_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Casein kinase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0410335</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8068122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LP43"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8068122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="165" mass="19235" checksum="AA764DE3F514970F" modified="2022-10-12" version="1">MIDRIEAFHNRCFLQSDIKPDKFLLEASSSPIVIYLIDYGLSKYYINSKGDHIQQISKASLIGTARNASISVHDEIEQLKYSKNSEFQVVIVNIILANVEILFIVLVLKLYKFICSQYTILYENKQFKENYSSLQNQNQQSFNQKLLHCQLDSQSIVYFLYSLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LT92</accession>
    <name>A0A8S1LT92_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0380097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LT92"/>
    <dbReference type="OMA" id="KQVKFAP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="108" mass="12914" checksum="8A0E7AAC4E4BA1F8" modified="2022-10-12" version="1">MNQIKGPKLNFSTPIKIQDFIEDKLYSVFDLESTDSFKNKKIQKQVKFAPTITIFLRYQDEDIYRFRDRLKTQVKMTKEIYNLNPTLEESPKKQQLKSCIRVPIDSDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LTC0</accession>
    <name>A0A8S1LTC0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0480082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LTC0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="303" mass="35539" checksum="3567A58161F340AB" modified="2022-10-12" version="1">MTQKPSHNQALFDKYIEYDGFEGEGFRIAQSDNQSDIYILDDYGGWVDKELNYYDRDGEPAGYFDKDYNFYNMTGYKISGGYKGGISIEREIKKKQKYLDSIPDNSKQKFVVEIQNLPSNTVPIQIINYILERCDLQQCEIEECKKVQKQATKGYVVVSGAEKVQKLLILEGRKFRLKEKDGKYLSNNIQLYVREAKFNNVKQYSDYTNNFKNNQQEYIEQQEDDGYKIVFDNLDCKKTNQELINWIVSQTNCQQNEIQIKRINQKSTGQIILKNENLMYLILKQNGRQFGDSCITFKQISKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3D5</accession>
    <name>A0A8S1M3D5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PPRIM_AZ9-3.1.T0490085</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3D5"/>
    <dbReference type="OMA" id="KRETIYG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000632">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000522">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="67"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="359"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="401"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="437"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="474"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAD8072361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="502" mass="58320" checksum="B1C5EA89E01B4002" modified="2022-10-12" version="1">MGNQCCTNQPTEADKQIETKPEDMSKLIQQSSKNEEEPLKQITSIPTLSVQPSLFIQMKRETIYGTYQVGKLLGEGAYGQVSIVTHRVTGMQRAMKAIRKDCLFEEEQAKLFSEMTILKNLDHPHIVNLFELYEDDKFYYLITEYLRGGELFDRIQKAKTFSEADAARYMKQVMQAVCYCHSKNIVHRDLKPENIIFASEDQYSILKVIDFGTSRKFEHNQNMSKRLGTPYYIAPEVLQKKYNEKCDVWSCGVILYILLAGYPPFYGRNESEIFDRILKATEWNKISKEAKNLITNMLCLDVEKRYSAQQVFDDPWLQQGQGANLIDDNFLKNLTEFSAKSKLKQALLTFMASQMIQPKEVEQIQELFKQLDRNGDGKLSKDELITAFQQKVQSGDRLIENMEIKINKIVTEIDVNMSGYIDYTEFIVACLKYEKLLTIEKIKQTFKIFDIDGDNYISKEELQHIMEGVDDDIWKQFLTECDQDNDGKISEEEFINLLSDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5F2</accession>
    <name>A0A8S1M5F2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0500156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5F2"/>
    <dbReference type="OMA" id="YCKSQKA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="291" mass="34111" checksum="DC7B8E270623B6B8" modified="2022-10-12" version="1">MKKAFTFADLSTSIQSGISNRQTQLSTSSVQIRSASLRKAIYRLKQENRISQMGISSISNTKVPKNQSYFEIKMEQKHNRISNPSQEPHMNINKFDHKQEIIRSINSLQQYCKSQKALEIAVQKYSLKKSFKGSSDLSTVSTFVDKINRDVVNRKSKVIQDVMKEELRKRIPKKQKEINYELDSMFKNYKSFSQLCLNENTSIQKEKVYNGYLKVYNSGKNDRIFLEKIEERKKSLSEESEEYRAFLGEIDEATIQRDVYKNNIRNPFLDDKVTVMQMKKDGFLKRRVTYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M845</accession>
    <name>A0A8S1M845_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0550162</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M845"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="59" mass="7099" checksum="58B7BD9382329A45" modified="2022-10-12" version="1">MINLSDSHLMQRQRKVISSFKILISLTYKQQIKSKKQEQVEKYQNSILKQFNDQQGTLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M895</accession>
    <name>A0A8S1M895_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0520064</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the exportin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M895"/>
    <dbReference type="OMA" id="IDSFCYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044189">
        <property type="entry name" value="XPO4/7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596">
        <property type="entry name" value="EXPORTIN 4,7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596:SF2">
        <property type="entry name" value="EXPORTIN-7 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8074071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1087" mass="127713" checksum="F781D1B95162CB65" modified="2022-10-12" version="1">MQTIMTLIEQKNQQVLELLRNFVLDYSNIELFQSLILEDCNPETTFVLYSIIQKCLINGDHFTNFPHQKVSTEFFYNEYDHRLNITEICTKHIEIALIYLQSSHNHPNYVYNQVIFTIALLIRKFWVDILKIEDIISQIINSTLQTNKIVFCKIFENIISCIRQYSYASGYIEYRKTLNNFQTLSLQRILQAIHLQIQQLQGESLIKLYGQNKIMGESFINLLYQLMNLNFNLSYYEIDVDNEGKDTSQLTNFPDYYLDLIIDPIFLKELFQDAIGFLNIDQNLSLYFISMLQRIACVRLSIFHNKKCKGGFRRVMWEGLIYLYQNQDQFINYVEFSSEIHSLAIRIINNLTLRKLTKFQLQFDNCFQLIQQVYQKTVQLFGVKIMPNSPIIKLQELWNQIRYQGSLNLPLHDGLNVKINQIMQNQLIIVLKTFFQQNQFTEIPQECSVKKFKKLLDITFMPFHCLFKSHIQDNIGLILQMIEQLRGDNEYQSAAVISLTIVFLLHTSNTEILNLGFDSYNFGQKEEPKPNLDGQAYILKFIVELIKNSQVQKNTRILKMAILSFIESFITVTIDSFCYEQETKQFKNLSQLYSQLIRADQRYQNYLLVLETMLDECFQVFTYGDPFLIEFSLKLIEYIYQKVKKYIPRKVFKTCSITDMLKQFFLQLNITCLNTPQYIKKRALVFKIITIVWVDDCSDDYVNALVDIYNQIKQSIINEINKVNILKYVWDLIGIASEIDVDNIYRTFLRIIYPDIAKLLSEENIELFAKDYDSSIAIITLFKAIFYNKNTRLSTENIQLILYQIYGKAMKFFIILIQQQIQTAKTQQKISDLQLKQVSKLMALMSQISGQKKINQGFFIIYNDQCFLELFNLQIQLLLIYRPLLQHIPKYKKLLYEFFDCICQDHSETLAYKCDLNSYLDIFHICKESLKDIIYDNHGEDTINENCSTLILIGTLLSNISTFLIQEASVGDINDLQMIKNKINAINISGKQILYEMFNLCCQITLIYPKNSKVIFAIPEILYAISIFNPSDFETLLISIMTNQIGIISNLLIDQISLAFKNLQGELQISNKEDFLRKIKVVLEMYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9G2</accession>
    <name>A0A8S1M9G2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0520201</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1220093</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9G2"/>
    <dbReference type="OMA" id="MFQVTDP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13437">
        <property type="entry name" value="SPFH_alloslipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="2">
        <location>
            <begin position="54"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8103905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="288" mass="32951" checksum="59056EBE4D3E6EF3" modified="2022-10-12" version="1">MNAHSRRKIAHLEVPEVQDNQTMIAYAKCQFCLGECFGCLRTWIPCIFCMCVNYPYQEVQQGTEGLFKRFGRHIKVVRPGLHYVNPCTDTLEQLDLRITVIDLDRQSVMTKDNVTISIDASVYYRIKTSRFAVYRVENYDQAVRQITYAVLKNTVGSFILQDLLEKRQEVADQIEDQVDEYVKDWGVLIDNIYMKDIQLSSDLQQALGSAATEQRLAQGKLISARADVESAKLMRQASEFLDSKTAMQVRYLETLQQLAGSNGTKVCFVPDEKNQEKLMHQITQGLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MA45</accession>
    <name>A0A8S1MA45_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cathepsin L</fullName>
            <ecNumber evidence="10">3.4.22.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0580083</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in extracellular digestion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.</text>
            <dbReference type="EC" id="3.4.22.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.15" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MA45"/>
    <dbReference type="OMA" id="MDCADDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000104">
        <property type="entry name" value="Cathepsin L 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035737172" description="cathepsin L" evidence="12">
        <location>
            <begin position="24"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="30"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="110"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8077378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="306" mass="34272" checksum="DBDA4C68A563ED80" modified="2022-10-12" version="1">MNKALIALLTTVLLATTNYLTQEDPLRTLYSEWKQQYQTRFASQFEDEYRFEIFKQNYNYYQQVNSKQSSYTLGLNQFATLTDEEFESIYLGGLDDTHVVVDESITSYNYPETADWSAKMNPIKNQGACGSCWSFSAVGAFEAYFIVNKGSYNVYAEQQLVDCDTYSYGCDGGFAGNAIDYLNKNGALLESQYPYTATTNKCQKATGTQKANGRKTWSTATQAYEAIAKYPISVSVHASNWKGYTGGIFNDCINTSTNHAVVAVGYDANGNWLIRNSWGTGWGLKGHIWLQSGNTCGVLRRLDQVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAD4</accession>
    <name>A0A8S1MAD4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WASH1 WAHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0510114</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WASH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAD4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071203">
        <property type="term" value="C:WASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043014">
        <property type="term" value="F:alpha-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028290">
        <property type="entry name" value="WASH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021854">
        <property type="entry name" value="WASH1_WAHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23331">
        <property type="entry name" value="CXYORF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23331:SF1">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11945">
        <property type="entry name" value="WASH_WAHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WASH1 WAHD" evidence="4">
        <location>
            <begin position="12"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="204"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="279"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="443"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="308"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="341"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="443"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="462"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8073536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="473" mass="53472" checksum="AC8471F055A02216" modified="2022-10-12" version="1">MAFIQPCPWQFNLASFDLHHKEGLDITLGNLSLLEQATNITEEKLDYRVNLVKARIQQLAERINVCNAKLNHLQNCPQAIVFQSPSKFVKKYEFDPTNKQSIFSECYNKPIIPIYEPYKYIRLANEQGYQGKAPDNIDRVATIQMAYGKFTQKGKTIFEGERAISKRQIQGYGTVPRKLKYVDGLSVFNQLINPYTHHTFDAREVFPEPAKQENKRKKQQADDEQVTMADAPNTIGDTITKYGEESYSYVPSNKPIAQLKVDAKLNLAGIVDYDPDNDDLDFFNPSTQQRNKKPVAPSIAPAQPPPQIQQTQQQQYQQYQQQSQQQQIQQPPPQQQQNKSAPPPPPPPPPPPPKGAPPPPPPPPPPPPPPPPPPPPGQLPPPPPPSGSRSKLLDDLNTDNPLARLKPVNKNKAVEQQKAQIKAPEMNHMDILKQQIAQRYQDLNRQPTRTTKTMRTQIIGQRVDDSDYSEHSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAN4</accession>
    <name>A0A8S1MAN4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0580084</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAN4"/>
    <dbReference type="OMA" id="ETYEGDH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="113"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8077380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="523" mass="60825" checksum="4639E6A6799F40B5" modified="2022-10-12" version="1">MKSQYDYSNVLFECILVRKHFICDVKYYCYILNDYMLMSRKTKDLAPKYVLPLQLTNQVGWIVERKDKKIQFKAFTIKYMEKYKDFVGSSNDLQKLKEHLRNKVTFYKIADFYEAQYNLGKGSSAKVIQIKELGNEQSKLAAKAIDKSYLQKSESGMQAFLNEVNILSHLSKQNDCTSFIKLYETYEGDHTFYLILDLMQGRTLADELDILKQRKEMFPMKLVKVIMKQLLQGVKILHDNNIIHRDLKPDNIMFRELDSYETLTIVDFGLSTFIDVPKYQFPKCGTPGYVAPEILNLVDRQQKYDKVCDIFSCGCIFYKLLFGHSLFMGNTFNEVLGQNKKCNYTLDGSEMNSIPYEAQELLKRMLVKNPSERITAQQALESDFFNKPSSPLQSKKLKTFNVSNISSRNIFQPTIEGLLSEAESPFQRISQKCKNQFDFDVDIQENKCSSIQIRQLPSVKKPTCVRLEDSTIGSFYAKDPLSSFKKLKGTSQKQLNETKEIKVNQFDIDQMQRISLFNRKEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MB41</accession>
    <name>A0A8S1MB41_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0570102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MB41"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="143" mass="16462" checksum="F3EB9B1B61CE3AB6" modified="2022-10-12" version="1">MECFTARISKLFEMNSDQKPAFQLEIFEINKVVNTIQSEYILLNESITLLNIHVYYKCPSNNKKVHSQLNKCNSCSGSNKIYNLNHYCHGSINTINIYAKFKAQTNYKELTLTTTSNSITIVQKLTNKIITQETILLVEFLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MB75</accession>
    <name>A0A8S1MB75_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0530262</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MB75"/>
    <dbReference type="OMA" id="RMIRIFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058565">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013935">
        <property type="entry name" value="Trs120_TRAPPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058563">
        <property type="entry name" value="Trs120_TRAPPC9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512:SF5">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26254">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08626">
        <property type="entry name" value="TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trs120/TRAPPC9 N-terminal" evidence="1">
        <location>
            <begin position="195"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 first Ig-like" evidence="2">
        <location>
            <begin position="475"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="992" mass="117171" checksum="BD189288A9F64C06" modified="2022-10-12" version="1">MNAFERTLEDMKSIKLLCKKLGETKQDQLDRMIRIFQSMPQKIYFSNFYQNQLYKNTMGVFSKKDWNSDSVTISVIDQEFQFDWPELMIYMKPFLMFFVIDLQEGLIPIDEINKQKKKVNDDKLIRFLLFNCGVNSFQNIEQEQTFIIIPPLSDDKMIYYLEDQLQLYLCQIIEYFASEILRLKNDQSQLVVFNMKEEKISDQSKLKKRKQGRQIKIMGDLALMINDPQDAIEYYKQALDNLNKNNDFLWCGIIQQHLAASKSSFEEIEEHFRESLIFLKKTKFINLEIECFFKLMQYRKASNDKLGLNKTIDLFTKTFDPESPIEKCKFYLFVSELYGQIQMKRKQAYYIRLASFQLATINKSMALELTKLSSTMYGLNSYFSDDQSNIFWTSLQHNFVQEIQHNFQGIPYFQHNTQIQRIQMFKQDFTQQQLETMLQILKQESVKHELKQTYLLMIPKIERLQIIPFKEKFSLINKEIKSQTEGQDIFIVNPWANKESKNELKYPLNSLIELVLYISNEYSFDFQLDKLVLQIEGSDCVTYPKGITLLANTKNHPVTFTIRPKSIGELKIIGIKIQYLNYEYIHKLNQFGFSALLSQDIQQQEQQLFNLHNIQIIEDIPNVQASIYRDSTLINEVEAVYFDPSELFTYQFIIKNKSEQLIQNFGFEIIVESEDPPIYEYKHIIPNFNLDGFSSINVLLSDFDLVNQNNQVHIKIPKIPHKTHVIKKISLTLFLWYNNALYQREIKLNRLIQIRQRTVIQQCQFVSKYRCNNENIKEIYDLQDSCFLSIQIVKKDFLISLELQSQILIDGQFNQILNEDCEYYTQALKLRKLPLNQLTNPDLKVIWKNLFNGNIGILNPFQHLSQEDIIKHCCNDLIDIECKESNKQIHYKLILHEKLTSSQSVSVIISLTNEYVNTNLSCAKSMVSSVQNINNPIITEGYTSFNLEIETNKQTQKNGQLGYYGQLNQSCLKFIVNDFENQRQYIKRCEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCT7</accession>
    <name>A0A8S1MCT7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0590138</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCT7"/>
    <dbReference type="OMA" id="MRSSEYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="20"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="390"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="390"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="407"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8078047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="442" mass="51084" checksum="A0A193CE8990DAFF" modified="2022-10-12" version="1">MNDVSLLIDELNDQILTDQFVYIELLGGGAFGKVVKAQSKELNQTVAIKIIERNKNKAQDQEASLLEKCNHKNIVHFHKLLYTHNHLYIIMEYLQGITLQEVMQKQLLENKVRNLIKQILEGLSYLHNQGIIHRDLKPSNIYLVRENNKSIVKLIDLGLSYQISSHKIANKQCGTLLYMAPELAQDVPYNQTVDIFAIGIILYQLFHDGQHPFFVSGMRSSEYFRRLAKLEFDLHFKENIPPMAKDFIQKTMALQPDDRMSAYQCLDHPWIKNVEQQQFPITTNEIISTFIVKQKFIKIIKALMVQTKLSQLSIKKDPKDSEGNRYTKTNMTEINSQQEQEFIHLRLPMSQRDKPPLSKQKMLKTMQGTFNTPQMSKREVTRQSLALSFMGSPNSLTPQSHSKRTLKTPSPTQFRLPSIKSPKQAQSPSIANIINKSRFFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ME85</accession>
    <name>A0A8S1ME85_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PSI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0530224</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ME85"/>
    <dbReference type="OMA" id="TINECRF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035932820" description="PSI domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="120"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="291"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8074926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="496" mass="56894" checksum="1BCE9C324040791D" modified="2022-10-12" version="1">MTYRVLFITLLIYSINCNVIIRTDARCVCKQWKLALECANDQDCIWNSNTKTCEQEECSSIKSQSICSADEGCQYRDGKCENFTKCEDLKGKTINECRFMSTNCRESNGEHCLPNALERKCSEFKNEGECLQGQDGFCLWLESKCILWNNCVQALTKSQCEMLPQSCDWSETLKFCLQKQCSEIDHEYDCIAVQEGPNSHLYQVCEWNYVLKQCESSIPDVLTFDTCASNTLQAYHWSSSNANEGFCEQCLSPNVQKPTPKHCLCNSISSQTDCQQNQTCIWKDSKCEERKCTEIDPPQACIQLEHCAWFSGSCVEFTQCENYKAFSNLECQSINKKCLLSDTLETCTSKYQECKSHKTDDKCNGSKDSKNEQCYWDEKTNTCQVWTQCSQQKQATYCEYSGACLWDGECKQITCKLLNQHSCTHYLTSPNSKNWKYCMLLDTCQDLNPDLLSKDECYAFSYGLSTWNSSECQLCKFPDDYTSILSFIGMIIITML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEJ1</accession>
    <name>A0A8S1MEJ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NadR/Ttd14 AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0510214</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEJ1"/>
    <dbReference type="OMA" id="MAIEDNF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038727">
        <property type="entry name" value="NadR/Ttd14_AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053227">
        <property type="entry name" value="TRPL-trafficking_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34932">
        <property type="entry name" value="TRPL TRANSLOCATION DEFECT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34932:SF1">
        <property type="entry name" value="TRPL TRANSLOCATION DEFECT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13521">
        <property type="entry name" value="AAA_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="NadR/Ttd14 AAA" evidence="3">
        <location>
            <begin position="88"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8073734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="512" mass="60147" checksum="6034A440FA9299FB" modified="2022-10-12" version="1">MNKDPLIYGLCFSLIPCLIVFTKWKLDQKKFAMKENLYKKMLFNSNELQQKKAEQEQKNLKSDAELGETIEYNLMNQLLKNNHSRVRKICITGGPCAGKTTGLNFLAEKLKERGFAVYIVPEAATTLFMGGGMLDLDNYSAEDKMTFQMNLLNLQMKLEHTFNVLAMLCPENKTPVLLCDRGLMDGKAYMDSSEWYEMIGKYNLDEFQMRDLRYDAIVHMVTAADSTDCYSTLNNKARHETQQQALEMDHRTQKAWTGHPNLIKIDNHSVKSFNEKLDWLLEEVLRILGHSIRQREIGITRLFFLDELIDLNKIKDEHQIFEIQKTYLIPQSENSTLLLVQRIHEGRSQLYIKEKFFENNTMVKANIVKLQKKDYQIFKRLANPDYLQLNLKKACFTYEGQYFIVNEYEGFCQFLKIKNCKKDTDIKFPSYINQQYMDEVFEKYTSRVFAKEFSKEPELIDRKKQGIKFRKLSVADDSIEKPPRSDQEPIDDFQLDQKQVISEKRIPQPKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEM3</accession>
    <name>A0A8S1MEM3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0510229</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEM3"/>
    <dbReference type="OMA" id="QSIYVGF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="2"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="181"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8073764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="32099" checksum="6A12EA5EEF3F55BB" modified="2022-10-12" version="1">MGDYIKALIETQVIGAFRVALTMPLEHVLDRIKTYKQSKQGITYIQSYLDIKGARGLIGLYDGFYPSFLRNMVKQYYRWPMMIFIPSMLNDHIHNQSINKIITGASIGLFESCIITPFERLKTLKMTSMATGFGYLKYITFESIYVGFRIQTTRQVVSWTNYLYWDHKVRYALKADPGQPLSIVNSLIASSLSSILNILAVHPFDTIKTLVQMEENQNYRKISLYQSFKIVYQNYGLAGLYAGWQARIIAYFCQALLTTPTIDYLERNYGISKSKKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEU2</accession>
    <name>A0A8S1MEU2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0570230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8077132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEU2"/>
    <dbReference type="OMA" id="IICHNIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="104" mass="12183" checksum="8B188134350BDD3D" modified="2022-10-12" version="1">MGLIICHNIHSKNKKVSFVQDNVEYQNRQGQKQHLDLPYRHYINKSNYIASNLHHGPEKFNLKGSVKLMNGIGHSSIMQRRFLEKINLNNIYFHSKPKILVNSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKI9</accession>
    <name>A0A8S1MKI9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0640196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKI9"/>
    <dbReference type="OMA" id="VAIWDSV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="214" mass="24707" checksum="7DEB7B042FE7C48D" modified="2022-10-12" version="1">MSISDARPLKIVVVGDSKVGKTTLIHNYLEQTQGRLSIQKQQLDYQIIQVQIQQNEYCVAIWDAVGLELQNQMNRFLVKDAHVVMLCIDLSKQILIQSIYTMMDTLISYCQEQCQFVLVGTKQDLNSQNTQILNQFFLELQQKYQNLKLLLTVSIYNEDSIKQAFQKTFELGASLDKSSITILQSFILQRNENNSVLKKYSNRESKAQKHDNCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKW1</accession>
    <name>A0A8S1MKW1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0660035</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKW1"/>
    <dbReference type="OMA" id="KKNKRGM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="6">
        <location>
            <begin position="121"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="201"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8081517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="259" mass="30037" checksum="26D5112EA35650CB" modified="2022-10-12" version="1">MPKAAKTSKAKKLQKRVADRKKNPLFVKEAKNFRIGNDVQPKRDLSRYVRWPRYILLHRQKKILLQRIKVPAAIHQFSKTLDKNQSSKVLNLLKKYQPETKTEKKQRLTKLAEQKAQAQKGESKKVQVLKFGLNHVTTLVETKKAKLVLIAYDVDPIELVVWLPQLCRRQEVPFCFIKNKARLGALVHQKTATCVALTEVRKEDQAEFDNLARDLRQHYNENHELLRTIGGGQVGIKSRHQQEALKKALELEELKKTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MMV7</accession>
    <name>A0A8S1MMV7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0590162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MMV7"/>
    <dbReference type="OMA" id="NDYNATI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002893">
        <property type="entry name" value="Rab_B04 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8078095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="218" mass="24830" checksum="A29AA84AF9321E10" modified="2022-10-12" version="1">MNSLDKSPLFKFIIIGDAGVGKSCLLMRYMKDNFTTEYNVTIGVEFLSKIVNVDQNTQVKLQIWDTAGQESFRSVVRSFYRKVAAVFLVYSITNKQSLERLDSWLKEAKDHSSPSIITVLVGAQNDRESEREVSYEEGKYWMETNGVNFFFETSSKTRENVEEAFTETAKLVFLNHINGGANNESKLPALNQTDLSVKKLHQPQEKSDIKNDENKSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MN17</accession>
    <name>A0A8S1MN17_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CENP-V/GFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0660081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MN17"/>
    <dbReference type="OMA" id="EMEVIQC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052355">
        <property type="entry name" value="CENP-V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620:SF1">
        <property type="entry name" value="CENP-V_GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620">
        <property type="entry name" value="CENTROMERE PROTEIN V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="1">
        <location>
            <begin position="5"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="123" mass="14141" checksum="9491900A805DBA85" modified="2022-10-12" version="1">MEHLFQGGCHCCKVQFEFSGPLEMEVIQCACSICRMKQNHHVLIPQSKFKLLTSMEELSLYTFNTKQAKHYFCKTCGVQSFFYPRSNPNMVAVTIYCLQLPQNVKLTKVTQGIEQSIKTIIQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPC0</accession>
    <name>A0A8S1MPC0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0630178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPC0"/>
    <dbReference type="OMA" id="QGEIEEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="302" mass="35108" checksum="792FCAFFEF149EF9" modified="2022-10-12" version="1">MTRKPSHNQALFDQYIQYDGPEGEGFRIAQSDSSTDIYIVDDYGGWVDKELNYYDRDGEPAGYFDQDYNFYDMNGFRIPGGYKGGKSIEREIKKKQKYLDSVPDNTKQKFVVEIQNLPPNTVPIQIINYILDRCDLKQGEIEECKKVQKQATKGYVVVTGADKVQKLLILEGRKFRLQEKDGKYTSNEIQIFVREAKFNNGKQFSNNSNYSKNNQQEQVEQEDDGYKIAFENLDTKKANQEFINWIVSQTNCKADEIELKRPNQKPSGQLTLQNENLMYLILKQNGRQFGDSRITFKQITKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPV4</accession>
    <name>A0A8S1MPV4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">cGMP-dependent protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPRIM_AZ9-3.1.T0670160</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPV4"/>
    <dbReference type="OMA" id="IVCYRNS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000068">
        <property type="entry name" value="cGMP-dependent protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0140">cGMP</keyword>
    <keyword evidence="12" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="210"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="396"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8082250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="662" mass="77187" checksum="27CCC3E039E0F3F5" modified="2022-10-12" version="1">MGCTSSIEQTTIISSKAGKPKLTKSMSTSSIVCYRNSKIRENVTILNQGKSQLDCQFITQVLKKHYIFYQLNEESIQNIIKHMFYCLIKQNHHLYQNINTPSCMFLIDKGQLKTQNFTLFQPGSIVGEDNLLLGSIKFIEAIEDTYMWCLDRIQFNKAIQYITSQSFITNRAFLQQQYLFQYFSQNQLDGIAANILPVQYQKNQIMFSDDAFVIIKNGEASLFKGNKFSKTLSRRDTYGTLYKLNKPKNTKVRAIIETQCLILTQPKLIEVIGFNIQKQIYQAIIRHVLIEIEIPGSEIEKILEKEQYKLKIYNNEQIKQRQIHEDLSIIVDGSVDCEGETLKLGEFISDQFDNSIYVANGTIAIIQRDSLYIEDYIIEESSDDEYIKCKPRFIELKQLQIIRRNSNNIINLVQFKQQNYILRQINKQTQQKTQQERRMKSQKKIFEILKCPNISKYYQFYEDNTSYYFLFQFIDGTLLSERRLTLPQIQSSILQMISILDACYKNSIICRSIKLDNFLIDYQANVYLINLLNAKQASRTYTIIGSPHYMAPEILEGRGYDCNSDVWSLGVCLYELLFESVPYGQALDDPYQIYEEIIGSSELEFPDSYKDEQGKSLMKQLINKNVYQRIGSNFDQLKNHSWFSSLDISNISPIESQIEIRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MRY8</accession>
    <name>A0A8S1MRY8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0620064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MRY8"/>
    <dbReference type="OMA" id="GEHQTCL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="306"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="353" mass="41202" checksum="B6FB11E263010886" modified="2022-10-12" version="1">MSNSPLTKVPTSAFSPIQALSVRKQEQIPQALSKQAIELQNTLNKLKEAKQKKQLSNSNIVVQSITNPVSSPQKNKHTEDSEPIQIQYKSLFKDIESPQICQPKTYNQMNDQEAELKYEERKKQIKEQIDRTKSNSKIIQKPKSSQKKAVEKQIQKIDLSKNSNKQQQQELVSKIQNITPQPKYIQHEQIKVMQKKLKSDIAKLDKEIQGEHQTCLFEIKNKGHDLYQQGLLIEDRKQFKKKLHEREKQQKEMQECTFKPQISQLKRMKNQSRQIESRQQQNESKQQIKCQSNRDGQITSKRLSRSQSQDQINSTTITQTINNNNDINSYLNRIQDKYQQIVNRANALKLKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTF2</accession>
    <name>A0A8S1MTF2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0690059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8082999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTF2"/>
    <dbReference type="OMA" id="ACRATPW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="422"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="62495" checksum="8DA7BFD08EB39415" modified="2022-10-12" version="1">MNWDFINRKLKEFFEQRRQQSIDKIFTLNTVTDLNIPKKQFLEMETQVTDDPKFTQMQQTIPLTLSTPKKTKTNPKSFSQISHLFSPHGPARSTASMQMRQIVASTLKNESNSYNSVRTKDIKKVQELVGQSSKTANKPNFRKLRKQQTVVSQKILKEFDNQFDQEPNFNIKQSDSLKCFITQTKNINTLAMRAQLKMKSYGSIRQLNQPSPTIDNFTKMNFAKSSDPRVGLDRFLSNKWLQFVIEQKSMRKDDQFNDYLKSYTESPQKLLNVQKLKQKQEVRQQFNNELPLQHFKPHRHTPLSAKNKQIKKETQGLVKSYSQTVFDIEKKKDDEELIEFYPIYYDKKVKRRRIIRKLKVLAKIAVIKQMFVYQLLEQQQQLIPYQSPGSVAFLRFVKYNNIHAAKQMLDQEKKLIGSFDELGQYAIHLAVMIQSIDMVKLLLCYGADVNSNDFFLQKPLYFALVTNNLEIAKLLLACRATPWDQHLRDYFGCCRSQEAKDLIKLAQYLKIYNRDPECLLNIDNIKQQLSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MU26</accession>
    <name>A0A8S1MU26_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0640179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MU26"/>
    <dbReference type="OMA" id="KYMEQTQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="71"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="278" mass="32583" checksum="F91A0D0EA99DB09A" modified="2022-10-12" version="1">MITTPDINDSTFHELQNEIQCLKSEKEEFKQTLLKLWIQISKTLNDDFEEILKLGINSDIEDIMNAIFIVEEARQEQINNERNQIVALNEQLKSHKNTTDSGNEELKQVEKQYRELSELVLQLKQQNVDKDLQISILNAETNKLKMEITQLSSKLNDLSAVLIQAKEEKIKYMEQTQNQIKVLMEKDLHNSRQIQMLQIQKIELENSLKKYNQKYTNEQSNIMYCQTQKEEDQTRMLSCSPRTTHIDFSKIVQQSLHNKKAEMVSGSSLSQIPYRFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MVZ5</accession>
    <name>A0A8S1MVZ5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0720033</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MVZ5"/>
    <dbReference type="OMA" id="MLTSAYD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000019">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF46">
        <property type="entry name" value="THREONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="250"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8084380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="609" mass="72034" checksum="1A0F3FEED40DF516" modified="2022-10-12" version="1">MKIIYKFTQQVLYQNKQYSILKPSQLSIPKEAFIAKITNKDGTTSLHDLNRELKQDCTIEFLDFTNQDARRVFWHSSAHVLGAILSKFGAELEFGPSTENGFYYQCKMDKEIELDQLNQEIEQYLKQKKHLFEIHNISYDEALQLFNKNSYKLSKIKQTALQNYQIYKLDDFIDLCKGPHIDHTNRIYKLIINHSQPYQNSYRYQGIAFPNSKLYQEYQNQQECDHRHIGKHQHLFVFNKLAPGSAFFLPHGTKIYNKLIDMIRKEYRERGYQEVITPQLFHKDLWKQSGHLDKYADNIYMTHDNQMGLKPMNCPGHCLLYKTLQHSYKELPIRFADFGILHRNEVHGTLTGLTRVRKFTQDDAHIFCTIEQIQQEIKNTLDFLSKVYGRFGFTWELTLSTRPDKFIGDLQIWDEAEKQLRIALSDTPYTENPGDGAFYGPKIDVLIRDHNKKQHQCGTIQLDFNLPERFDLTYHHENDKFSRPVMIHRAILGSIERMMAILTEQYNGKWPFWLSPRQVLIIPVSEISKSYAKEIYDQLYKLEFEVELEDTEQTLSKRIRKGEMLHFNYILTVGAKEQESKTVDVRERGDNHKHQSMKLDEFITKIIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MX91</accession>
    <name>A0A8S1MX91_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0730084</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8084978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MX91"/>
    <dbReference type="OMA" id="HLEQTDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="417"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="446"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="697"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="730"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="784"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="862"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="5">
        <location>
            <begin position="138"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="4">
        <location>
            <begin position="259"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8084978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="883" mass="103718" checksum="D94CE8829F9D9527" modified="2022-10-12" version="1">MQNEKDQIHSPLLQNHRESEQRAQMIEMQVFRSLELNDFEKHQKKDEILEIESNKLQQILEAKSQWKTLLKGWRKDGNKKQQNDDNYFGFQYNKLLEIFDMNQILKEAQSTLTKHDKEKDLKEKKLKIQIEKLNQLGGIYGLQNFLKTCIKQGLDDSNDYDLKMREKYFGKNEKPQIAIKSVLQIIIEQFQKERLFQLLLIITTINIIIYFHEDMQIYLFYIIMLIIMVVYKGVIKQQYNKEIVQHQNDIKERSKHLIKRCNVLRNGDDTIQIHNSQLVVGDILIFKQGDFIQCDGIITKLSNCNGIEVLQPFPNLFNTHEVKNIDEVKESNCSQNDEQMQSNISLIDNFIFAGSQIIKGSGQLLVCSVGQQSSDYIQYKQSFLEDTKTEKQRTTYMKQNSMYNPFAFKIESLMNKIGIQCIISLLILLIILLFRNTYLINITQILLYLSQLSIITIPSQLLQLINLFMIYVIKKCRQRSIYIQDYLSLYWLGQVNEMIIDKIDNKVTMFSKSQINIRLFTQLQLEEVQKTAIQSGLVKEDEIDDKYIEGTLLSSSFEQKNKFVFLSRRLTCPTLDHLKLEKIQSSKTLEWLEQQKQKFQFQGIIDQQKLYEEIKNKNIIIGGNADRKEQLINLLKLYDKIIFIKGEENDGKAMEMAHVSIGPHESRSCIKLFNHLEQTDYSYISSMYECISQGRAVYFYILSYSYAQACTSQTIMIFAIASAILSDNIYIMPIPTFIIFYLALNHFSHQVQKQCNMDVQKYVMHRGPYTKQGRIIDRNTMRKLYRLHSILIVELLIAYYYGYFQSVQDVYIGLLFSKHVFIMVRPIKQVLQQIEGIIYGLIINLINQFINKSNQDYHSISVLLRNYSIGLIVVAFNLFVGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZD4</accession>
    <name>A0A8S1MZD4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TRAFD1/XAF1 zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0720207</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZD4"/>
    <dbReference type="OMA" id="HETYCIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051986">
        <property type="entry name" value="Innate_Immune_Apopt_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049439">
        <property type="entry name" value="TRAFD1-XIAF1_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16295:SF10">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16295">
        <property type="entry name" value="TRAF-TYPE ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21366">
        <property type="entry name" value="TRAFD1-XIAF1_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23580">
        <property type="entry name" value="Znf_XAF1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAFD1/XAF1 zinc finger" evidence="5">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="224"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="292"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="257"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="327"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8084682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="438" mass="52130" checksum="D990FC0F7F2A2A03" modified="2022-10-12" version="1">MNENCSPCSNCNQLIADTKLILHETYCIRYNIKCERCGQYYDKNDPESHEEDYHKKEKCQYCYQEFDDLSKHKCQKTPKLCLYCELSYPMDQIFQHENQCGSRTEKCQMCQNYIMKRDLSKHYQNCSQQIEVKEKQIQRQSSFQDKVQNLNNEIIEVDQQHKSQQKQSNIDTQPQQIKIQKQQQQQQQLINKLPQKEINFGQFHKQTLLKHQVFQDNKKKEQIGIDKQLINKPNRPPSSNSLLGSRHNNKNTKPSTSQNRIRQQSNNNNNQKLVNPKGEVKNLELQFEKKQLTSKTKNSNLPPLGKQIQNPKRNLEHKNNNQDVRTQSLQSRQQSKRSNQNITVKKEQIEIEGLKFSEDELMQQKLIYEQLKYQKRDLNMKEQQQHLVKRQNSNSKPKVSIDHSEVVDQENFDQFMSPEERAMQQLILDNYKLNSKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0U8</accession>
    <name>A0A8S1N0U8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0760066</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0U8"/>
    <dbReference type="OMA" id="FKTQFML"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="117"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8086220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="206" mass="24757" checksum="BD91158E36844FFF" modified="2022-10-12" version="1">MNKSRDWNIVDDELNRKLKQLQELKSSLDDQSTELLLQNKDQNQEYNNDINYYKEFWRYYILNEMTIKKVNELHTQNQKLHELIVEIDKLQQELHQALSYRHKKKNRRTSQEIEKSFICPYEKCNKQYGSDVSLNLHIKLKHDGGNKTDREKFAKMIIEAQQNGETITDLNINIKFPPGYLDQFKTQFMLSQQNQLNSERKSIEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1H8</accession>
    <name>A0A8S1N1H8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0700139</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1H8"/>
    <dbReference type="OMA" id="ANIAMYN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034464">
        <property type="term" value="C:BBSome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002786">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002806">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028796">
        <property type="entry name" value="BBS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44177">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44177:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="245"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="281"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="46436" checksum="4C448AF078BA435A" modified="2022-10-12" version="1">MGRLQSGFARPITNRQDTQENAVKTARVGTRLGTARPMTQGGRYIRLGTASLQQIGDQFIMVDKLDLKKMADKPVISRILCDYLIYVEHNPRKALQLAAEATQVNNFKDWWWKERLGKAYFMLGLYREAEKQFESSLKLQEMIKTQLQLAKVYLRIDQPITAIELYKKNSERYPHEISYLIGIARVYDQLNQPEKALPFYQNTLIKDNCNIESVASLGAQHFYLDQPETALQFYKRLLQLGLYTAEIWNNIGLCCFYSGQYDLFYSCFERALSVADDQQRAEIWYNLSHIFINLGDSGTAYQCLKISLCYDPHHGEAYNNLAVVQSKRKEFEKAKLNFQKAIESNEFIYEPIYNLSVLHFNQQDYSQCYLLVQKTLKLFPDHSDSLELNEKVLQVLQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N216</accession>
    <name>A0A8S1N216_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0770022</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N216"/>
    <dbReference type="OMA" id="SCQANVG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LITAF" evidence="6">
        <location>
            <begin position="71"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8086640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="158" mass="17810" checksum="96F3917B9BF87D5C" modified="2022-10-12" version="1">MQNNQHVQLQQEMTPTPSEGSISQVVYPSIQNQQIQIGQPIQQQIPAQQIIHQVPYQQSPYSQNEQPIYNPQQPIQIIQPIYSKYPSIITCVYCQRQVQTVVNYEPGTGTYVVGGILAAVGLWLGCCLIPCFIQDCKDAVHFCPSCQANVGKKRFIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7D0</accession>
    <name>A0A8S1N7D0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0810083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7D0"/>
    <dbReference type="OMA" id="ECNIDYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="370" mass="44453" checksum="D1CF397297BE4899" modified="2022-10-12" version="1">MKNFLEKHISNDYSIRMNNLSQNHEYQLNQYKCSSPIVFGNRFASPSPFRSVTPIQGQLSPANQSRRESAFSRNSDNYDFSVKEKSKILNKPITQYYKKQQDQQDEEECNIDYFFDKEQIIIEEQELLHAKLRMIRDDFNFKIQQLEDFLREYLRERQSILQNLSISIKEEQDRYNEQSKQNKRKNKQNEIHARTDSINKQDSKSDSQPIQQEIIRAASSMKEQRTEFNYEKRDCFDIRRESRHESINGYSSHHRRESSYKTVKLRESRVKREESLKGDSVKEEQPLKSALKKKDSNRQTYNCQDDSQDDWKFIKEAKNNLEKQKTQLDDKQSPNKVRFVNQEKSQKKQQDKVVKSKYLNDIDIFLKCRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N8D4</accession>
    <name>A0A8S1N8D4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0780190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N8D4"/>
    <dbReference type="OMA" id="LPPKWIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="125" mass="14495" checksum="3868A46C24758350" modified="2022-10-12" version="1">MISIMVVCLDSEYESQCPLVGIRILRSLSIIPIMNEVKKSIIPMIKELPPKWIRPLILSSFMMAIALIISLIYTLLKITLSLLMNLLYIILIVLILLISFGIIKNTIHIPQNLQQYFEYIKFIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDD0</accession>
    <name>A0A8S1NDD0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0860156</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDD0"/>
    <dbReference type="OMA" id="KIIQLPW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052187">
        <property type="entry name" value="MFSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035782769" description="Major facilitator superfamily (MFS) profile domain-containing protein" evidence="8">
        <location>
            <begin position="32"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="1"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8090680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="435" mass="48928" checksum="18B8510F85FAE0E7" modified="2022-10-12" version="1">MDKKQVSWHQTSLRWLFLAMTCVFQVGCCLCSDFPSVLASQMKLQFSIQQSDINQLFTFYSLPNVIFPFFGGIIIDKIGIRTSLVSFTAFLVFGQALCYYGSLILNYNYLKIGMMFMGMGGEICMSVAQSSIVSKWFVGQEMALAFGLKLTFTRLGSVLGVNLLRYTYVQFNESFQSCMMICCLIILIVWGVSFIQIEMDKLSDFRDGKIKKDEGQSNVSLSDVKQFKLDFYLVSLTCVLSYSAFIVLQQNCLQMFKIRYQLQDKEATFFYSLPYYISAIITPIFGGFVDKIGQRPLIILVSGVLLLISTSLYCVELNCINDTCHNLTLLAQTISGLSFAIFAPVIWPCIPLCIKDNAQGTGFGVVTSIQNIGLSLLPLIVGQIIQDETQYSYVQMMQFIRIVTILGTLCNITLYMHDKKSGNKLSAKSIKQKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NG14</accession>
    <name>A0A8S1NG14_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0850167</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NG14"/>
    <dbReference type="OMA" id="DEICFDN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000134">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="30"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="94"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="232"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8090239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="348" mass="40183" checksum="C4C4BF919A4B7356" modified="2022-10-12" version="1">MFNFSPIKQKQYLSASNQFEQPLDDMSCIIENGSVGSLYLGNIESASSLENLKRHKIKGVLSICMNKIPFDVQTSLQHYLHIYLEDCESENIARHFENSNQFIDKARQSGNVLVHCMAGISRSATLVAAYLMKKNNMSAQDAIRLLERKRWQVYPNNGFLRQLQQYEKVLLQQNGRSDISSPLRDSWSKQFQTPTKDSLFYNKYEEQQYSSQKTRPLDEICFDNYKRKSLGYDDQLRSPETNEKKPFSLTEHRPNSATRTSPDLIKKKNLLADAIHGNKKDGLAALALELNSIDWQSKKLDFESKYSKPSIQQNNTKSFLTPNRPLQLNQHQSKLDELLNSFNRKSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGN7</accession>
    <name>A0A8S1NGN7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein phosphatase methylesterase 1</fullName>
            <shortName evidence="3">PME-1</shortName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0890027</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Demethylates proteins that have been reversibly carboxymethylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGN7"/>
    <dbReference type="OMA" id="GHNIHED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051723">
        <property type="term" value="F:protein methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000643">
        <property type="entry name" value="Protein phosphatase methylesterase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016812">
        <property type="entry name" value="PPase_methylesterase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022950">
        <property type="entry name" value="PPase_methylesterase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="52"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR022950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8091720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="323" mass="37638" checksum="F717F3B0A3769901" modified="2022-10-12" version="1">MNGKPQSILQQQLGTLQPNLRNHNLNWNLYYENLVIFNGTPIYWVMKSEPIILLIHGAGHCAMTFALLIQELKTFCSCFSYDIISHGLSQRNETLTMKNLISECESVIEFIRIKNPNTNIILLGHSLGAAIASKIKHQSYIRGLIVIDMIESKAFESIQIMDYQLRKRPSLFSSYSSAINYHVYNNLIRNPISAQITVPHYLNENLEWKVDLIGTKQYWEQWFEGIQNGFDTFPYQKMLFIAESDRIPTDQFLKSRYPIHLFEKSGHNIHEDEPIRMAKHISDFIYQQRVPINSCEIEKLKQLGIIGFRPKVLQYKIELKDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NIL5</accession>
    <name>A0A8S1NIL5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0890143</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NIL5"/>
    <dbReference type="OMA" id="HSYIKTC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000051">
        <property type="entry name" value="Protein phosphatase 2C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="4">
        <location>
            <begin position="121"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="97"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8091952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="401" mass="45010" checksum="68C9280763B3B692" modified="2022-10-12" version="1">MHPQWSSFAKLQRDNSLMPVGMYSPQKANLLRLLPRYPESVDVGPTSSKFQGNVSLPALPPLKKLSKIEQTVLIYKGKVNKLPSLQLSDQQLQRKKQISETDGIRKSITPERRQGKRNSVQFAMRTKAGCQPNKATKINQDAAILCPKNLENMGYKFFAVCDGHGQYGHMVSNQIKQQLPKHLGKQLKETSDLDDQISKAFAITNKELWNSEIDTNLSGSTTVSLLIKKDMIYSANVGDSRAIMCRFDNGWKVVELSKDQKPDDPQEKIRILDAGGRVEQQKDFHGNGVGPFRVWLSYIQAPGLAMTRSFGDKVGAQAGVIAEPEIKQFSISAQDQFIVIASDGVWEYMSNEEVMSIVIPFLEKDNPDQAAERIIIEATQAWRRNSLARDDITCIVIFLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJ33</accession>
    <name>A0A8S1NJ33_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0900143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJ33"/>
    <dbReference type="OMA" id="SHIIIEL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="428" mass="50103" checksum="50A5655AAAEF30EA" modified="2022-10-12" version="1">MQKSFVLGNKIVCSNEAHKKQNMQIIALCLSDDCNFKKTLCPQCFNEHDKSHIIIELSDLTSQLEEQQTFQIQQRIEKYQLLSSQQNICFKLIQEKLNQMLNEANQAKQLSEQKIEAESKQHFANNLRNEEELEQLTKELQNKVNTDHFNPRLIKLLNHKNILFGYERYITKLLFDTSLFLNNTDCVEQCEYFMDNQLMSLKNLINNINEIRQEIIVGFQDEEQNLLYMQSILEKQDQNFQLYISQKPQQISDEQIQYMNNLIKLPIGQILHLYPTEALTNNQFQIIFDNPFSKVITKQNLNHLKSISNDKSLICIGAIKQSDPNLLILCSIDYATEVLQSTSQFKVARKSKSGSVYWYNKYGVFCGVIGFSPTQQIDIGNALNIHYDTHIFEGDLRFSIQVDHKSNCRVGKEIIDNQNYRWQIYLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJT0</accession>
    <name>A0A8S1NJT0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0930079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJT0"/>
    <dbReference type="OMA" id="TTWCCRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15841">
        <property type="entry name" value="SNARE_Qc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="31819" checksum="97447D30D032952F" modified="2022-10-12" version="1">MAASLHVQFYLDKLMSIDKELGGTAAAQKKAGDDFTSLKDSIHTLLFQLRSKIEQKQKYANVQRNVETIKIDVEIKDLITDIESLLDKLNDALRKQSKDKKKYPEQNIQAKQKIYSNYRQQLYDLQQLLDPNAVIDLQQTNNSVETVSALKMKLMDQDGRKPPPSQQMTQEEKDAMQRWKQNDEIMDKQLDVLIDGTQQWKENAQKIGQKIDHTTNQIDKLTVQVDKTNEELYKQNSQLKKIVEKYRQPNKFCLDMVLIFILIGLCGVIYVILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NN12</accession>
    <name>A0A8S1NN12_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Chromate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0960128</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chromate ion transporter (CHR) (TC 2.A.51) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NN12"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015109">
        <property type="term" value="F:chromate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014047">
        <property type="entry name" value="Chr_Tranpt_l_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003370">
        <property type="entry name" value="Chromate_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33567">
        <property type="entry name" value="CHROMATE ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33567:SF3">
        <property type="entry name" value="CHROMATE ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02417">
        <property type="entry name" value="Chromate_transp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004810">
        <property type="entry name" value="ChrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="381"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="404"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="429"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8094647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="50671" checksum="A5C1B200E43C3D66" modified="2022-10-12" version="1">MIEMQTLGVKNTQPKFSLLIDDSKTLTYLELLKCAAPLALVDVQTLSTSHYQQFKNVFVTQNKAVSEPQFEEITSLVDFIPGATTSQMMVALGTISKKNALGGIVALLAYTMPGAMILTILGYIYSKYPDPRDLNILVFLAIQGIKSSTVSLILQLLISMAVKTINNKMHISLSILSAILFVTYQQPYVLILTMMMGGIITIMVEYDAENTIGRRTFVKEQSATPAIFNDPKLINKKNSHKISQLLGNKSLHMYSLILLLLLYLNSQQQSLILDLSLSFYNIGTFMIGGTPITLPFMYAETALNPLYINADAFWLGFGLAAALPGPYLNFAIFLGTHSMGIYGGICCWVAIFVPSVLQIWGLLPHWSNFRKNIYLMKFKEGVISVAIGFLATAVYYGWVDACKYDLSTATVISFLSLVYVSLLNFNSPAVLTLGAALFIIRYLALWYYRELDPVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPV7</accession>
    <name>A0A8S1NPV7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0930054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPV7"/>
    <dbReference type="OMA" id="LIIEPQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8093399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="28160" checksum="4310DFD23F2CD347" modified="2022-10-12" version="1">MQTCQLCLKNTALIQLICSHHICLKCTNKNKQLQQSQLQNKYWIQCLKCQKKTFSYDFSNLLIESHELSLLTDRSQHQFQLNESQLIIEPQMFQRRRSMNAIDLFTKNTSPIRQEKSQNTTQKLKQSIINLNKQNVLISSVQFKKISDKKPTHKRCQTGIIENKQVLNSMLNFKKLTKQKTSPIQKCCSIFDYLLSLSYQHTKTSDRKQFSLANSSKIDSKKNIKSRCHSQMQIKQKIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NR45</accession>
    <name>A0A8S1NR45_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0960026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="670" mass="78107" checksum="336977748909C814" modified="2022-10-12" version="1" fragment="single">MIKYIQRSLFRISEEYEYLKCKQIFKSQLEQFKKALNKAIQLQKQIDQNTQAAFKMQNHAFFTSNVPAKTILAAFLQKREYSNEEIVGQTFVQLGHALKARGDRTYFDHKQIETSMGFDVLIKDLLKLNDYRVLAEAIHGLSLTGVDLYSDQIVDKVFQQLDAKELNPDEKQLIEAFLLEIFEEKQEIPVNNLIYEVEKLIEQTQSDVFNVLEAIVEIRRVYLKLLEIQKNDWVHVNTNIKMMEFEEALVSQGLLHPAEIENEDVSLSLETALSYISGKDPDLMAFLICILEMQLIHRDQVKPIEPKLSDFGDLMVAVSEYKGHEIAQRLKNAQPLLVHSDKYKLAKAFANAGLNTESREYLAQVTDEELLNSLDSLHLHLQLNKTDRKFPLPDNLTILSLDELIAYATYLALQNQNYEAFIEELKFRVKNLNTIRDLNEKQQIRIDVLEKHSGQKLIYEEKDINHSELYKAIKDTLPKSVQLIEIYDLSVNNEINGYAQLRVGSQPYMTLYDFLGKDKEQFKQKLQLAFNLENSDIILFNKIKDRLESVQVEENSKELTSQTIFNAQWQYLKKRALNYLNKNDNLNKLKLKQTLLDLQERLRIVNQNNVFQDLIDEIKVQPIKFPKWYGMIPSQSIYNPKSTIEGFKASLITNRIYDPTYCPEQDLFXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NVB1</accession>
    <name>A0A8S1NVB1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation channel family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0980093</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035906338" description="Cation channel family protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="1918"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="906"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="943"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="979"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1007"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1044"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1104"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1129"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1296"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1329"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1374"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1396"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1434"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1490"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1515"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="418"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="10">
        <location>
            <begin position="674"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="1663"/>
            <end position="1730"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8095279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1918" mass="226478" checksum="8716BB0A443BB1F1" modified="2022-10-12" version="1">MLLKTKLLLTQLMLQNLISLIQAGNQQTKEMLVLTQNAIGILLNLIIFCLIYQKQREDIINIVIQFQIIIQLEIIFYNVHSINNYFLLIYILTIENILKNSNKCYQILTHLLCLYISIRMMIQFYQQQTMFEFILFVFWQPLNHALLHLEKQFLEKTTTSPNNPTQKQANPHAHLSYGDQFQQDFLDEKSPNPEKNVSVTLDEDLLNCLPYGLALIDKNYHVLNHNKKLLNYLMVQTTEQIVNSLDQLLQNAELNSFKTHLHAKYKPTIVPLRKLKQSSANQYQVRSGRTLNQTNWLSDRLKVNLKSKESFQSIIHDEVTYSYVQFVMNEFQSNIHRQMSNGQDSQSMTSETTHMLQFHVVQDVKSKKKHFQIKVYEVKLQVKSIDPVYLFVIENITNKEELKELTFRFKFQQALLNSFSHELRTPLNCSLPLMEVLSKKIDQELYETLLLPAITSSKRLLLQINDILDYAQIECQDFKLNLGKFCVTDILNDLKQLFQSECQQKQIELILNFNSSLQLFSDKLRVTQILVNLLNNSIKFTKQGGRIVLSVKKRDNQYVFSVWDNGEGISSDQMLNIQNKLFEQNGSNKLGLGLRVSQGIVKYLGGDGELQIKSEKGFYTVVSFSIDEQIQIGIKEVESSIKDVEEIASNSKDLAQRVYVSSKKFLNQQCKCPQILIVDDVPFNHIALLALLQAFGWKADSAYDGDCAIRKVKQKLHNTCCKIFRLIFMDIEMPGKNGFVVSSERSRKFFEKKDKRLLQQCVQLIMDKKMPKKPMRVVCMKQYLNQFHQIPCKNFQENIFVDIFYLIKIKIYQSSYNYNQIPQKLQLFFLMFISLEQPLLSKKQVESNRISKNVQMQIFNYVQQDENVSQLEDVPIATKKFLRYTNGIEAYLNAFPSFNPDHWAIFSWRLFICFIVIIYFYLIPVLMFFGYEIISENLRIENIAVFLYIMLSFLIIDIFITLNTGYYDKGQIVLDKHSIFVRYVTQEFLFDIIAILALILQVSFRDHYAFLIWIPYLFYFKYYYVTLVDSQVEQLLQVQRKLRAFYQILKLVLTIFFLVNLFACLFYAAGDYWINQRQDYGSWLISSGLQNDIYLLPATIKYEYSFYWALTTMLTVGYGDVTGKNPLEIFVSIITMIFACVIFAMIVSAFQNVFSEITQHNMEFDKKMTDINRFMKDKNIDLETXQHQLIIQIKIKIYQSSYNYNQIPQKLQLFFLMFISLEQPLLSKKQVESNRISKNVQMQIFNYVQQDENVSQLEDVPIATKKFLRYTNGIEAYLNAFPSFNPDHWAIFSWRLFICFIVIIYFYLIPVLMFFGYEIISENLRIENIAVFLYIMLSFLIIDIFITLNTGYYDKGQIVLDKHSIFVRYVTQEFLFDIIAILALILQVSFRDHYAFLIWIPYLFYFKYYYVTLVDSQVEQLLQVQRKLRAFYQILKLVLTIFFLVNLFACLFYAAGDYWINQRQDYGSWLISSGLQNDIYLLPATIKYEYSFYWALTTMLTVGYGDVTGKNPLEIFVSIITMIFACVIFAMIVSAFQNVFSEITQHNMEFDKKMTDINRFMKDKNIDLETQQKVRRFFSFMFSEKVRDFNEQIQIINQLGPQLKDEVIMQSYGLIFKRTFWTSQFSLDFQKKIAYLISEQMYCDGEMVFKQNLFSLNGNNDDDCLYCVSIGHIQLYLQFEDQDMNFYEAEQDSMFGELGFLTGNPRSLSAKSTTISQLYKLKRLEFIQLLQQFPLDFQYFCQLRDQILFGQYNLINLSCFCCQSSSHLANDCPKSHYYPNKIFLINRIMNNQQERQQFKRNNVRRDNLNLIKIQKAQQQVKQELSLLYEQEDIDQLSKSLSVDFMHDTQSQISEQDINPEFDLVNEIVNRLEQKKKQALIEIDIKCSYKNYYPKYNAEYIIQNFKKERRFTRAFTRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXT4</accession>
    <name>A0A8S1NXT4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1000115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="TYSIHAI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1354"/>
            <end position="1377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1406" mass="165487" checksum="28498206ADFC1F62" modified="2022-10-12" version="1">MMFILLLIPYVINAKSNFINIGKTYDGQFNFKTNHSLGHSYYFEISELLNNADIIIIVSQQTPQGDPNIFLSLLNPEPYSIEQSELGYCQSQGSDLCVIDKKHIQIDKPYYIGITCSEDCKYTLKVNYDQEQIMLMNDLLRFKMNERTSSQIVRIQIDHTLNIEELEITGRIINYFEIENPFHMYLNLGDILPTSQESDYVGKDFWHGHKYLILPWIQIQNSSILTIIVESQIGSLIELQTQTYEKIRKSVYESMIQGEVKLNQFHHYQIDKQQNQNPQNYLQIQLKPYSGLLTVYFNFEQELPQSLEDFEYKYIINQTSIITFSDMDLIKYDKIESIDIIIYGNELSSYQFKVSPQNNLGILSQNVNYQGRVYKDKLLSFLLDARNDIMSGKLNLQMESENNNVVIIIKKSMFQQEIITNDNIKNMDKLESENPSSLFIFTKKDKQNLLFELDFPIISQNVQIGLYSIGIFLNESNTYFSTYSFILKSELQFKRLKDNTPYKSVITKESYSYFQYVLNPIEYLEEIQIMLTSIQGTSILLSSTKDYPNETHYEQIGENNLITYYFKETLQNNQVYFISVYCQTAGIFTITVIIKNNNNNQEKLGAYPWEYTQIFQGDSQYHVLNNQTVGLFKIDLTEQLEIKQRQFYGLNKKKKLISIHILSPLGGIMMYGFVSPSTNSNNSIWSNPKEISVKLNQLNGEQTVFLRIELQEHYNYSTYKIALHSQFQSTNHIQEIIENEHYFGYIDQKGQLLLFRYYQKYDYSIIKNSEDKENDVRVTVFLRKEDLSSQESTLLIPKEKLEDIECRKEDQELNYCSFLIQITAKKPSFYTLIISHENNQISLHMDQIIHHKLPKENDHYYSFIDERDSNIIVRNLNQNVQLQILVTLFQPQNIQTNIEYPYPLNETQSNLLKISKNFKGFHISSVSIFKDDIAKTYNCSTRCMIAITIRQIPNFNYIIDSNSVYSIIYSSGSRQLSLKSFLPGKISKGYTAYYSVQITEEDVELIIFLEDSNLCNATIIISKDEFPNEEKHDWVYNHLDQKNISISNYNNNIYENMKGYYYIGVLGESECNYYITYYLNDPKEKQLQEQLQDDNFQIPYLLIQNQQQTINSINPILWKFECQTFGRLLIQLQYLPYNINIIGLYDTEKYNQNYIPIEIIFKNDLQLIGYFTIQKLGFYYLQTSIENSIIDIVSEPIYQIRVALIREDSILPDFQCLTKNLISEIIQSDDKIIINVNPLRIELMNSSFQIEQIKYVLLFGDSNSNNKSVYFHNIDNIDNVKQQKRQIQQFNTRTTKIVSKRYLDTNLSFICQDIQYINTTYSIHAIATFTLYNLERIEIDYFYDQKFVMINQSIGYQFIFGIIVLIIIIIVILLIIFRKWQERRKVSLVKNVQQNSIEMIFKNIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYM7</accession>
    <name>A0A8S1NYM7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">PA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0970080</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYM7"/>
    <dbReference type="OMA" id="ECEVIFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02123">
        <property type="entry name" value="PA_C_RZF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000050">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056858">
        <property type="entry name" value="VSR_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044744">
        <property type="entry name" value="ZNRF4/RNF13/RNF167_PA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702:SF1">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25011">
        <property type="entry name" value="VSR_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035790010" description="PA domain-containing protein" evidence="13">
        <location>
            <begin position="17"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="14">
        <location>
            <begin position="59"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar sorting receptor thioredoxin-like" evidence="15">
        <location>
            <begin position="198"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF25011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8094883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="52267" checksum="4EFAD133A26F7FB8" modified="2022-10-12" version="1">MQFILLIVCIVAFVDCKMKVLRPPDLVDRLGSKVNIALANFGQIPFGHRLMGYVDMAQPNDGCIPLQSTIGSQFIMIERGNCTFVTKVRNAQNAGYSLAIIGNNNDEPLQSDFVMADDGQGYSVNIPSIFISLRDFNIMKEYAQNYKVLDNSDEKIFILVKFDVEKKDQIDVIFSLDVNDRDSYRVVDEYSQYYNLLQNENINYTLVYSIFNSNTTELDYIIDEDNCICSRRYCSVDPDGEGIASGRNVVEEAIRQTCIFKLFPDKYFQYMNQFNFQCTKAQAYSTCGSKIITNLKLSADEIIKCRDESFKDPLNDQTTKNLSNAYNAILEHQLIVREQAGMVGLPSVVVNSIVYKGQLTGKGVFGEICNSFIIPPLVCKSEVENFYQFEHQSQHYLFLVILLVSILIAIFIIILFFLFKKFVQRDSVEVTQVQVNEMVSQYIKFYEGKDQQKQNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P429</accession>
    <name>A0A8S1P429_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1050007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P429"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8097730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="34824" checksum="3DB7B57E9E94A032" modified="2022-10-12" version="1">MDNKVGQSSEQNNISLLIEINELGVVKISLSIKGMLQQKQLVIRIGQFCQNFLDSWDHFIYIQGCENRSIEAKQQFGKLTELHELLVIQINLSAYEMNINKIKSHNRCLRFFTNITYSSEVTAGIYPFNKLQNFQGQPLIFNQYNIIIIALIMLFFELAKFNGLIQSRFFDPNEIDTHIEIKKISYSQNKILSEEIVIAQTIESISIIFYWQCIDQELLKIQLFCLEQCCPQTNIKCDIKKILTVRLQAKFLLNQNSQNYIKISKTSVSLSATQVIKQSNDYEKLLFKLEKLNRKIKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P9N6</accession>
    <name>A0A8S1P9N6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIPF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1100141</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P9N6"/>
    <dbReference type="OMA" id="FVAMMMA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF1">
        <property type="entry name" value="PROTEIN YIPF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="7">
        <location>
            <begin position="59"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8099789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="202" mass="22944" checksum="AD2D6CEF7A7449ED" modified="2022-10-12" version="1">MDYTNQKNPQNYSQMEDLKTLDLEESVSETIMRDLKMIAYKLKYVLIPKEQEDNGKELRNWDLWGPLIFCLALAMTLSFKADSTTNSSKSDVFAIIFVLIWVGAFVVTLNAQLLGGKVSFFQSVCLLGYCVFPINIEAIIIAFIGSYLPFVVKLIPVIICFAWSAYSSVGFMASLVPPHKKKLAVYPVFLFYLFLSWFSLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PA02</accession>
    <name>A0A8S1PA02_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1110032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8099932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ESDHKIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1867" mass="221016" checksum="621495C1246913BF" modified="2022-10-12" version="1">MSIKDIQKLQSLFQINNSVVQEYLGIRLINQTLIDQLVNSDSANEIQKIKKIEGCKDWDKLKHKLLAYFMASQLTYGKPINKYIQQAYEDFLIQYHIFYEGLIEFQTCSMLNQYLILKQKLLKNDQNLKMEFRQLINMLIWKTNQLHFLIRIIIGYIGYVKISKQIGNVERVFEQISKLIEKSLKNGANYNHLLFKMDIELFSLYWNKIIQVSNNETNALILMQLLQQSQFYFNDYKFQSDIIKTLKMLQQQKLSINSNSQINEEIKLKSKIRKPILQINNLMPEEIPKQSTKSINKYLDLGKIVKTEQTQTSATSKKLIKNSASKLLNSKYQHIEEDPIFQIQIENYHINKIVRKGCFKIQKPVTLSTCTQRYKTQPSAQEELPLLTFQDFEQDHQDSILELPSPVKPATESDHKISGKDHSITHSQSSQGKIDKLNNLSNPYKLKNLISSRFQQILDIAQLSASSTPKNADLGKSIRRQKTISIKKEHKEFQLQPQQLSIPIIQQIPSQKEPDPLTSERRRSGMVVSSPDAASQISQAIQKSSYIESIPTKSQIIIAIPQSVQQQLQQNQQQTPQLNPKRPQRRNILTLRTATTFFMEQSNTYIPKFQFSLCYHSRTILWSCGLQITSADNILIAFQDTTNNKIVYRAQLQNLQIEGIKNWAQIIEVFIKEYQSQASQCLPYLRYNTIMSNRKSDNQNFIMFPKYQFEVTKNEIEQMGILKDLFRWMYTTYLYEQINITGIRLKLYTVEKDKTLNAYRELINVVNLYRRMIIQSTLCDEKEKFNLLICSYGECIKNNIILLNRILNFKINKQKDQQYMIHKQLVNLKNLSLNNDEENIFFIIKVSIIFQKENILYYKKNDYHTKYQVDVLFEAINSPIKIKPLRLQFDAIEKILEKRYHNNLKQIINQIFFSQRKCWHIFYEFIDQKLMINEQKQLQVKMNIFSLHDAQKMEACGMKLGFLIDLQTKVKPFAITEIERYIYDDIQKKLDYQSKSQTIHNREHMFDKHFAQIFGEDISNNEQHHFHQTTLIDLVKRYQPNINAEAKNEIITKKVGPIVIYQENPFTDIHIDDLEKLKSQFFFRQNLQLNKIQKYRIKLKHLAIQISLIKKQIILIYHCNQRLQRFCINPSDEEDNLLQNIVQLIQNGPICWVIQMLYKKISQRSLKQSFSSSQLQDGLELLKYKKSKSQLAEIPSYNVLHYYILNNYMVLLKYFTLKQFVIVQKIKNYGKYQITIKVFFRCQKPDILNENDYFLMRIDFQNIMLKTGMMKMIFNKYDITEMLKIDIPQNMTDANIVQTAMNIFQKCHLGTFTLSNVPFFVVQQDDLKQNESQITHNSYEDHSTKINVQQIRRIGNFPENTTFKKSDLCKGFLEQYIKNSRLIVTHIKKSYLIHSISTGQLIKISNNKVTQLLNDQQQITDYVLIQIYRHLLLDIWIVKIYVPKTTRQMVGLLRSEDFKENDGKDLMDSIIIYKKQAILSFKDLDSFTDITPRQSVMMQKSNHRLSQPRISQFHSPAVSSIQAQNQMAQSQVIPYYLSDFFYITRLVTEFTEYHLENGNKEQYIIKEKLIWVNIINNNFSLEQHPKQQNCFLSLLFDQKEINLQELLFQRYIRIERVGESHIEFYMKYSQKTDYQLKGIALLKGKINQLHLHNSKFPSIISEAKLNEEQIKFLKRTCIFFPFDAISYANTSNFNIELKYFSYNKFTEVKEQINLRELLNLYIADGFYKYQTNYMNSKLNHSDIISICYYMIHKIKTQNFLNLSQYHTIPNLNQLKTQKQSNKIQKPIRLQIKTFKKPNIYIISLLYNEVQVTSINWKSCQQFNDVFTFSYLNEHILHFKHLVDNEPEIALQRISKIIDKLMNNILIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PA47</accession>
    <name>A0A8S1PA47_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1110065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8099972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PA47"/>
    <dbReference type="OMA" id="ILLPQIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8099972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="121" mass="14761" checksum="555DC60B01E14412" modified="2022-10-12" version="1">MNHFKKRLGPQNDLDHILLPQICDYRSLSINKETIVHPEEKERKQDQRQCGILQFSFINSNYSHSERFRTKPQHMMNIERRKKMLKLQLIRVCDSIKHVSHHRKHYDTLQEIHFIKYKKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBX4</accession>
    <name>A0A8S1PBX4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1120146</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PBX4"/>
    <dbReference type="OMA" id="NDMHFKL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000226">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="15"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="436"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="93"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8100499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="598" mass="69163" checksum="A0552B413BB46DE0" modified="2022-10-12" version="1">MQQSKLNKSSLQQQSIKVYVRVRPLLKKEQGQECIIKTDQQKYVVIEQSTHILKQEYDQVFDEKVSQAEIFKFISPTIHKALQGYNTTIMAYGQTGSGKTHTMFGSDWEYSLNISLGHQSTFIDDLQTDQNHAGMIPRTIYTLFNNLQQGYYVYCSFLQIYNEKIYDLLQDHKIPQALQIHESKTEGIYVEQLTEYAVNNLYDCLTLMKRGEKNRMIRQTTMNLKSSRSHTIFQLLIETSKADSGGQLLRSKINLCDLAGSEKINKQDSMTNAHLIELKSINQSLTTLGKVIQNLSQNNKNINKLPIPYRESKITRLLQDSLGGNTLTNIFVNVAPNIYNIDETANSLKFAQRARNITQIVQANQINATDQELVNKLLKEIDYLKQLLNMKRKGLNSNDMHFKLMKIQEENERLRQANLSVNEVEKLMQENRKMREELQKLQSQSHENSESSHLMKHGSEEGFSGSEVDKQQYNTYQQTKLSNNGLLIVSNPSMQKTEVTIRYKDRGNKMQTKLGYVEASDQMLQREKRLMSERKRVIERLELLEQLQFRNSLNSSLPINGKIPKSSKKNSFNLLNKTVEEERTSLPKLKLAERKLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PD38</accession>
    <name>A0A8S1PD38_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1140107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8101096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CAICAAG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052798">
        <property type="entry name" value="Giardia_VSA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23275">
        <property type="entry name" value="CABRIOLET.-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23275:SF102">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_170, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035850889" evidence="1">
        <location>
            <begin position="19"/>
            <end position="1123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1123" mass="118922" checksum="F05537A65DEA9D6D" modified="2022-10-12" version="1">MRVLFCIALVLVVKGAITVTCPKNGLGCVTYGANIVIQTTRTNSLCRNGYYWNNQACMPCKNADIVVGGYYTCSAYYEGTSLTCNSGYTLINGVCVQIPLGCTAYGLNQNSDAYVCTACLSDYTLKAGVCINNRYCSSWDDNAVCTSCQTGYFLNWDLKFASTSANSNNQTDWINTYQTYYKFPGESFCSVCSQTMANCKSCNSAYTCTQCNDGYYWQQSISSTQLTSSNAQFAGSCVSCLTSGNCSTCQGSSCTQCKTGYYPTKDTTSTVILSCNLCPNGCSTCTSSTFCTDCYTSFYKYTDNLCYPLNKCASINTQLTTIPTTPKCSSCIAGYALDPSGTGICYQCGNCDSCTIPTGQQTASLQTSCTTCWDTYFGVKDTNGIVTCKPCTQASDSFLRCQGPVENSSLNGVVPTQCQDGYYLYTLPASGTTLAQTVCVPSNQSQQCVTIVATSATQYTCATCTIQTKLYTNSQCLACDAGLTNTLPQGCTACSGSPTNSIACSACAANYYFSSGTTTTCTACDVNCLQCAAGPTCTKCAITYYVSGGGCTQCGVANCATCNAQTPSSCQVCLDGYFLSSINTGTATTSYCLKCPFECATCSAPGQVCNSCISGYVLQNGGCVSLSSANCAEGYTTSTLTNQQNPTTQNGCAICKYGFYNFEKRCLQSVQPYAGAVYVQKNIGIGSNPGVSNYETIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIYNFRYFGVKDTNGIVTCKPCTQASDSFLRCQGPVENSSLNGVVPTQCQDGYYLYTLPASGTTLAQTVCVPSNQSQQCVTIVATSATQYTCATCTIQTKLYTNSQCLACDAGLTNTLPQGCTACSGSPTNSIACSACAANYYFSSGTTTTCTACDVNCLQCAAGPTCTKCAITYYVSGGGCTQCGVANCATCNAQTPSSCQVCLDGYFLSSINTGTATTSYCLKCPFECATCSAPGQVCNSCISGYVLQNGGCVSLSSANCAEGYTTSTLTNQQNPTTQNGCAICKYGFYNFEKRCLQSVQPYAGAVYVQKNIGIGSNPGVSNYETIIYSIFLVLMLLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PNN8</accession>
    <name>A0A8S1PNN8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1260040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PNN8"/>
    <dbReference type="OMA" id="NSAQFIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035715345" evidence="1">
        <location>
            <begin position="17"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="382" mass="42038" checksum="51D3C6C6CA8073AA" modified="2022-10-12" version="1">MKKLVILTLLIALALSQRLSINDANCNKYLLKFGTANNPDGTGATKLAFTGDVQFVSGTTNVKVNLRYSDVDLFNDTTYFGLVQEDGKTAATTCLDLKLWKFTSNAYSDPVQVTDLPITPSNNFQKQWRYYSFTIPGDQLATRLVQTKNANQFIYKGYYAVAYYAAGTNEVQYTFYFEFSVTVDRASGASVDTSFKPLSQTSTLGCAPNQACTAKADTVLKWCTDLTCTQFSTPDLHLNDQFVLQQVVTTAGMNYYLTGTEVWYTGNGLNKKATPISVNNNIKGQAIIQLRAEIAWSSVTIRVTSTLSDNQAGGRRLLVQTTYDPVTGETDEITCIKAEGKETCPTCEEECSAQGFAKDGCPECSSTQILAFAFMLLLFIMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PPJ9</accession>
    <name>A0A8S1PPJ9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1230158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PPJ9"/>
    <dbReference type="OMA" id="KEEMWIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="336" mass="39685" checksum="958C2A2C247DA598" modified="2022-10-12" version="1">MNMNIENEIDLEEVYDNKCYYKFHDQHRVQKDKWCKAFTFNKDDTTLVMGYNQIIRVYQINKGELKYVQSILGHNFDVNCLQQLNNSNQIISGGHDRKFLFWPINLISKSKFIIKLVVHHLPITCLIKNSQENLLISGSMDQTIKFLELNLQNQVWKCSQVLKEHNDTIFALSLSQNENQLISCGYDNLILVLQKSSSQWIVKQKIFASVHGYRLCFITDYLFTFQPYNKEEMWIYDNRNSLSQFIMTKSIHAKSDCQDFGTLFPQQFIPKKAMLLAKHGHYVHLIKLQDSEDFKIQQSINYRDQCIYGQLSNNGEYLVSWDNKSNLIKIRKYVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PS71</accession>
    <name>A0A8S1PS71_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1270093</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PS71"/>
    <dbReference type="OMA" id="ILWIIEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46726">
        <property type="entry name" value="TWO PORE CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46726:SF1">
        <property type="entry name" value="TWO-PORE CALCIUM CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="494"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="566"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="634"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="664"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="357"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8105479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1176" mass="138313" checksum="7031CCC8BB25A358" modified="2022-10-12" version="1">MKKDKRSDKRKSSQASLYRSSITITDIPDTLIEKNDILAEEIQKTVKTLTFQQRIAKAERHIYDAYFLRTIRNDIDETNKKFLKILWIIENQQFWIQFQFLLTLIYQLMTFFESSATDGLEGFLLAFFGLDTIITIILLKSKKKGGLGFKFNLKRQLKIFFYCMCLLDFFIHLGDPSIVRFSRILRAFLMPLYSKDLRRTLKGILKASRDLFLLIVLYLFIISIFSFVGINLIGELDNVDKTTQDYGNFLKLFSMLLMTATLDFYPDILIPPMMEGTYYALFFIVYLLLFIFLFAPIPLAVVYEGFRNHRMEIAISDIIKQKSAMMASFISLDFEDQGYISETQFKDFIRGFYRNQLSEHKLLQLFGEIDKDFNDKVQFDEFYQLLKVLQDGTRFNLPQAKPLECWERLRAFVNQRGLQRFIEGSLFGTIMLVVTITNCILIIAAFFIEDLSVLDIFNQLDTIFLVLYSMECLVKIVAIGIREYFNEGWNVFDISLVILQIIFDYVLFNVVSGNIVQSIKANRILRLAKIQKVFRLFRAFRSVKIISFLLKGVEFLDVVRRLLYKILFCIPIIFRLMMPVQMVFFIYATIGIYVYGNVETVPDNPYANSKCDPSQFQYSWGDCKYADFKSFAGAYLMMLQFFIAAEWNQVVFELTYDTGDMFSAMIFIGSFEFLSIFLLALISGLVWEVFTIVSLSIKQEDEQIGDEIDISNEAQLDTKINGISGVTQLKKKTRILNDDNPDVIQFQSNDNRDQKEGNPAFRTISEQFQQDTFNENNLQTREDSQVRIVQIAHSKTIIRIKTPEYKPYDVGALYKQHFKDYQIFLELKQSNDGFDVQHVTTEYMNHLRNEIKKDEIFHKKYINISNHHLLIQNAVLRDASEVYIKKQEDEFFKTSYREKFERIQELKFQSKFKIESKILFSLMGILKFPKPNIKYYFQILYLIENYFTYQLLPESSFFKLLHQINNKWYLISIEDMQICFNRLHSGPWDHSDTLFTTSNLQICENLRSFIDQDTYESRTTVEDFKLSLSKLAKNFEMDISSVDVNSSCILYQITNDQYAYDTNSIKLRRNQDEISPDQKFVVLESQRNIGTKTCFTHRQDSQSPDEHKHINKTIMFVLSKTQAQEQRIIYQNQVMLAQFILDLAGVIHNYSHNFFQQIEDLYTLRLKQKGKGIKRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSI1</accession>
    <name>A0A8S1PSI1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1290021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8105972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSI1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="70" mass="8821" checksum="764757E3C63B2651" modified="2022-10-12" version="1">MKRIILIHLNCLYKRFQFQKLIMQNIHKAILENWDLTHFDNFEIFRRELLIYNKLIFCLFFQGYNCQIHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PWR1</accession>
    <name>A0A8S1PWR1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1320075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8106959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PWR1"/>
    <dbReference type="OMA" id="CEQQRNN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="223"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8106959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="278" mass="32884" checksum="FFA666F15824EAD0" modified="2022-10-12" version="1">MQSNLPIESVLDALDNGEKLKCIHGLVVTLENMKSNQVHNTYLIKKIKELRLVSQIINDLLCSEEVIHSPKRQQSKIKKSRIIFDKQLDIQLLKSSIVKIELSIREGFKLHQELHPKFQLIQNYILEDQDDDQFNHLDIMRLVQIYQSEMEYVNEYFNRIEKNPNNSNSQSLDILNQSYQEMLLDQNIFIITQYYQNLNQMIEALENLKQKLVQYDEFESQSTNFENSRNSSTNEKHFPRSKSQTYELCEQQRNNVLKEITNEINQKSSETCINCSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4Q6</accession>
    <name>A0A8S1Q4Q6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PIPK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1420107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000146">
        <property type="protein sequence ID" value="CAD8109995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q4Q6"/>
    <dbReference type="OMA" id="LILECMF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="648" mass="74585" checksum="B77EDAB92A27122E" modified="2022-10-12" version="1">MIENEDSTFRIFIILLNALSFTLTILILLFICRIKQLHERQGHTIQIVLILECMFSFFLILSQFYYDEEDIYETTFYVKSEGQGCLAIGILNIFTSFAYFTNINLYSLESLGILSSKFTKNYKLRHLAVVSGSTFFALLAFLSNAIGFNKIGMCSLLPGNLFTIFLLILTSVTFIIIVWTCTQIHRLRKVLGKDLTSRIQDKKYKVNLIYIITFLIIRILPVLILTIISLADSDTETYTVAFYVLAIPMSLGGVCLSLIRSQEPTIQDYLTRKLQKQKSGIVELSLPKMEVRALSNSISNLSAESQIIKTHYLSYLQGTTRDNSVAYRKTCLDNEELVAKLTSQMENNQIKFKLNQFLFIFKIIREMNNGFIIPITIMNLTHYHMKHMRQRIFYFDCDSDLNASQIVCTSYAESVFIQISSIDQFMTSFDEKLNKNFVEQLHNSKLKLQTNSLTTYDGEVLISIINNQAKRYLTRHLLSAIYNLSVSAQRSFIPPLIGLYSIVNDDDQYSILIQRNLLNSIVLEQQGFKIQGLFTYQVGQVDEEIKGGSDTYGKMKMNPKVKQEFLYTLNQDLMILRDQFLWGYSFYVIYLEAEEGINVLPNMYKTRNGYIIASLGGILDQFWIFNNETIANPELYASQLAQQIDLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q602</accession>
    <name>A0A8S1Q602_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1440082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q602"/>
    <dbReference type="OMA" id="WLKSWIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="148" mass="17842" checksum="881244B30453717D" modified="2022-10-12" version="1">MLIYKKATAQELQKYREDQVKSWKKEKDLQRLTETQVNQMMDLFLLFASQRTFTIRTKDVEAQNTLLRVNEESTLFQNVLSKMILVDEDLNFINFLKLYLQCIHNKKNNQKNCIQLQLRKGQSLWKTQKDWLKSQITIILKRKTQMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q750</accession>
    <name>A0A8S1Q750_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1470065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q750"/>
    <dbReference type="OMA" id="KIYCKTV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="162" mass="19557" checksum="349CD22A2EDD7C19" modified="2022-10-12" version="1">MRQDSPRFRAALLHSIEQERSKKLMDFNELLKDNELTYLKFAFKTEERQENIYIKQFQKGTNFQRTGTMNIGDLWSNAQINRYLDISRFTKDKFLLQKLAKQNNKFTYIRKPNTFFSQTPRPQPVEKKDLKSIITELNILHKENKKLRSQINRKIYCKTVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8K6</accession>
    <name>A0A8S1Q8K6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1490090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8K6"/>
    <dbReference type="OMA" id="ALYGTWR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000011">
        <property type="entry name" value="50S ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025755">
        <property type="entry name" value="Ribos_uL4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14374">
        <property type="entry name" value="Ribos_L4_asso_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL4 C-terminal" evidence="6">
        <location>
            <begin position="278"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8111888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="406" mass="44950" checksum="744F6E6A73A91621" modified="2022-10-12" version="1">MTSVPLVSVFSADDSSKVTAKHIPLPAVFQTPIRPDIVSFVHTNIAKNRRQAHAVNPQAGMQHSAESWGTGRAVARIPRVSGSGTHRSGQAAFGNQCRKGRMSLPIKVWRRWHRRVNIKQKRHAAATAVAATGIVPLVLARGHRISQVPQIPLVVEDKIESYEKTTDALNFLKRFGAFEDVQKVVSTKVVRAGISKQRGKKYRVRKGPLVVYFNENAKLLKAFRNVPGVEVVNVTRLNLLQLAPGGQLGRFVIWTQSAFAHLDKLFGTYRYSSIIKDGYQLLRPLITNPDLARIINSNQVQEKVQPAKTTKVLHEVQKKNPLKNTKAMDRLNPYAKKQRAAAAAAIKANTKGTKKIKKNKALKKASRAAYNKVAASLNDATKAAVQEEQDIKVKYFSVQKGAAQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9M2</accession>
    <name>A0A8S1Q9M2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1490022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9M2"/>
    <dbReference type="OMA" id="QIMCDIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="254" mass="29241" checksum="97D89FFAF72585F9" modified="2022-10-12" version="1">MFSEATKEGKSDQLVPQLNQNPSSTFFVYNCYIPVVQDFMNQPPTLQVRNSSESFPQILKQTDSLQMGHLGGGMSIPSFQFGASTPFINAEDVMRNVQPQTAQNHFASFILNEQLKPQNQPQKNRIIEGTQIMCDIEIVLISRYFDMKATVFCSPNNKYNLKEDTSISNSEWLKDRMSYRYKKPIKESFCDMIEVIQKEGEANMKGIVQPIKFQQNGFDSIERQVPKSVKLEKPFSLFILEIQQLVSQFIFNTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDA5</accession>
    <name>A0A8S1QDA5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1560058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDA5"/>
    <dbReference type="OMA" id="EYFVLQC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="376" mass="44019" checksum="DDCFD1C6EFDE0860" modified="2022-10-12" version="1">MGNSSRHQNQQGIQSSQQTKQINKSESELIKYLAQSCSTFPSSITKNPEFKAQKVNCLRCNHILFDDGLGRTNKCEECQIPYVIKDGVITLLNHNQDVQCCPYGFPLQHLDSVVLRVNQNCINHISYGQLMSQIILPFFEKRERIITSGACFNIDQFEFRVVGCGSQLQGIVSTGTKIYCYESYTDRILYKLKIYTQRKNFQDELFHYFFSHPKENQIIQDSYIKINNQEYFVLQCTEQSGRLHLYTKMECISNVPRLSQVQFIILKQPIEFSHNNRNYAMQQVLNYVVHPYFAGLTRYLEKGQILRIGDFIFEVCLQEDYGFVIPNQTHIQITDQIDQRMNLQQFQSLKTTIQQADQLIIKIMIDKIRLNPYIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF57</accession>
    <name>A0A8S1QF57_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1600081</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF57"/>
    <dbReference type="OMA" id="EADLECF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF147">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8114579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="884" mass="104986" checksum="6FD208676E34F349" modified="2022-10-12" version="1">MRKNIKSASYHQQHGIKLEIKQKSLSIQSILKRTQQDNLNKSIEITMRQSIKSLKQFVSKLIEDLNQIGYRTFLIVFLTETQCNINMFNQLTFQVIFDKRQFFVIQKYKQTCYSINKQRIPEFLKLYIQKNLGSNKRIRNKQDDIQIKCQENFIDQQQVILRIRSINNFQMNQNQRKQRVCLFDIQVKLRINCRQREVRRLITFNRMQRNNGRMLYQDWETKDKRILELQLNLQSKIDIEQNFMSISNQINIFLLVYQFLDCIRNQGKESMRVSKIDYQYLLINNCYEFYLLIIYTMQQDDSQTQLEVTKPKNKVKKSKEKLIINVSSSQYPVIRYVGKKMLNWKIQKEENATNWDVWWTDGAVFSDLLGRMNAHQKVNHFPGMYTLARKNHLGRNLMKMHKQFPQAYNYFPYTWLLPAELSDFRANIGKNRTFIIKPQASCQGKGIMLVKDAEGLSIHEHYVAQRYLSKPYLIDGLKFDLRIYVLLAGCDPLRIYIFKEGLARFATEQYKKPGKDNLDNICMHLTNYAVNKDNENFVFNQSDKQMDVGHKRSMTSVFELLKSRGENVDQLWNTIKKMMIKTFCAVQPILAHQYKSCQPNNHMNNMCFEVLGMDVILDHKLKPYLLEVNHSPSFTTDTPLDATIKRQLISQSLILMNCTQKAKQEIINQERQILQQRMLTNKPTKLTLEEKQKLIKQCQEIRDQYENQNIGNFEKVYPLEQYEEEYDKFIEYAGILYQESTGANIKKIQQQQQQQQQQQQQQQQQQQQSFKRIEKPEIKSEYTLRTLEQKSDTKILEQKTELIRLKSEKSSGMKRILKKEHSTTLLADDTIRNIKFTGLKPQQKAILKQDIIKKNFKLPVSQGTFITPKLFMINENNKNSNNPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QIC7</accession>
    <name>A0A8S1QIC7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1590044</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1590045</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000164">
        <property type="protein sequence ID" value="CAD8114300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000164">
        <property type="protein sequence ID" value="CAD8114302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QIC7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036273309" evidence="2">
        <location>
            <begin position="17"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="61"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8114300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8114302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="319" mass="36486" checksum="A191C3746090231B" modified="2022-10-12" version="1">MNRIGFGLLCIMLVTASSNVMLQTADHSAFIDVFKTYSKQSEFGGRLFGELLMMVQKGETLQSLNDHVEELRKHLADDESEDEKYFNEKQQEMEATFSGLANEVEELRVSVEQAASRIQVLNNQQTIIDGLFANDQQAYQKRVGDQNAILDVIGQLLLRVSELQQGGGHSLIEKDNLLDEIKALGRGKHIEVLAQITAHLQTEQVEHIQELLNRLRDAVLNSLAIDEEKEQENIRLYNKLSGEIRSSLAQSDRLYQELDAHFQQKELELNTKKLAVREVGQTLQDRNDQRQFILDRVQEAQKLLNDNLDRIARWSVESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJ01</accession>
    <name>A0A8S1QJ01_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1650079</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000172">
        <property type="protein sequence ID" value="CAD8115632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJ01"/>
    <dbReference type="OMA" id="CQINVCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF65">
        <property type="entry name" value="FERREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8115632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13176" checksum="6EE2BF3EBD171DF9" modified="2022-10-12" version="1">MQRIHNFFKFSKNICFYVQSEINPQIKLTINAIEGEILAKELKRHKVPLPFECGFSCSCSTCSILLANSNDFNNIQIAQPQSQEEINVLTTEGKGGRVRLSCQINVCKELEGVTLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QL91</accession>
    <name>A0A8S1QL91_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1780011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000187">
        <property type="protein sequence ID" value="CAD8116728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QL91"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="260" mass="31167" checksum="DE13EC5DC39377E5" modified="2022-10-12" version="1">MKKLSQQKFQKLNFPNNYIKIFKELQDLLEILKKFQILIYEYNQKSENLITSLVYTEKLLLNMPHSLQNFELYQKQKSSKIVPARKVITQIGNKNQRINVSKNQKSFKNQSQQKPFFLDIYRNKVKRWKINDEISLDVTDILLNDVPKQFFTIDLDWEASQPWVENMRISSLKKEIEGFKLTYLHFQNLDNLDDLDILDLVFGSGYVSLLLFTLALKRKQMLQIQFHLVDHHHHAIKFLTEIKYRIFAFKQNVQHLILFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQ39</accession>
    <name>A0A8S1QQ39_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2080012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000217">
        <property type="protein sequence ID" value="CAD8117756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQ39"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="36" mass="3945" checksum="5A7AFD5C88EBB8E7" modified="2022-10-12" version="1">MIVDYDTGALACEYFSDESIDSCTAIIAEINNLCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN63</accession>
    <name>A0A8S1JN63_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JN63"/>
    <dbReference type="OMA" id="IKYCLDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33828:SF2">
        <property type="entry name" value="NUCLEOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33828">
        <property type="entry name" value="OS05G0596200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="169" mass="19866" checksum="F7D56ACC7DF99CF2" modified="2022-10-12" version="1">MPPKKGKGKKKQDSDDDESEEAYEPKATKKIQKQTQKKQQGSAKKQKEEDSGDSSQSSEESNEKQHKNKKTQPQKQNKTTNNTKKKQIKEDDDDENSESVENDKDNFKQYQKKETPPEDDPCRIYYESLYRERPESLLAIKYCLDYGLIDEANIQKAHKFYEKHKSKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQD1</accession>
    <name>A0A8S1JQD1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0060139</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQD1"/>
    <dbReference type="OMA" id="VNSQNQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="13"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8044057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="512" mass="59510" checksum="34DA25214513CD55" modified="2022-10-12" version="1">MATVQQNIFNNQFVIQKKISSGSFGVVYQGFDLRTSDHVAIKIEKPEIDDLFSLDREVQILRILHGIPQVPKLKWAGKDKGNNVMVIQLLGRDLTHYLRQRKRFSLACVLGIADQMLTILEAIHSKGIIHRDIKPENILTGKDREQNIIYLVDYGIAKQFKDKDDKHIPLRENKPFLGTSRYASIAAHRGQELSRKDDLESLGYMLIFLLKGSLPWQSICYKNEDEKVKMVGLMKMRITSQELCKDIPTEFMRFIDLVKKMSYREKPDYRYFQQLFRRVSIQQQIESRFDWYDENNYEKKSGSTSPKKQQSIKILDQSYLIQQIYTPNKKKLSEEFDDTGQSKRDSNGRQSLINNNSNNLLLYDSRMNLKNKSVSSYNGSNLQYSDIQPDLSCLIAYQRNLRYGSFHNEVEIQKQPDRSTSAQDKIKVKHKLSLDVKPNPHKSIIEFQLGLINNPSIMDFTVNSQNQMNEDESPCLDDKFSILKISSIDNKFKNPIQIFSLNLKDRKGIKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JR61</accession>
    <name>A0A8S1JR61_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0090118</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JR61"/>
    <dbReference type="OMA" id="QVIMEEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001016">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8045093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="463" mass="52673" checksum="2A69EF1628A38A8E" modified="2022-10-12" version="1">MKNSQKLGGIKKLQQNSNHEDLVDQEQICQTLKDEIIQILRMDEDHIAVIDTINALRSENFIESHKAIIKLRRTLSNFDQAEQIAKENELIPILFHIIQFGSTYLLKLEAAQIISILAAGRSEITRIIMQNGILQLSLTVLDGNQSELIMLIITILGKLAGESVKYRDSILQACSIEKIISKMESKYKSIYIWCLANLCIGRPSPKFDKIQSAFEIFAQVIMEEFNQQNLKMINDAIWALGYMIDGEPNRIAALINSGVVPQLINLLPMGNFVSILRIFECIFYGTEDQINYLLECGFIAHIRMIVDPKSREKSGDMIQTFAATETLFKVLSNQNFLVSLFRSLCDDNSNLRGDVLEVIGNAIQHGTNEDINHLVKNGLIKYLLRMFEIANEKEIASNHLKKGLEMLIKIIRKGEIKAKDNKSNQFFIIFVQLDGINVIQKLLSYKQDDVAKYALIFKQEFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JU17</accession>
    <name>A0A8S1JU17_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0100214</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JU17"/>
    <dbReference type="OMA" id="YYEMCHY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20507">
        <property type="entry name" value="CYCLIN_CCNB1-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000001">
        <property type="entry name" value="G2/mitotic-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="119"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="6">
        <location>
            <begin position="212"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="216"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8046162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="336" mass="39900" checksum="DAD0EFFEA925E2E3" modified="2022-10-12" version="1">MLQEQYRFFGKEIVNSSGEFYKENIDRNKSKHLTVVPRYLKLNLWCGEKENQMEEEPNINQLCQFDQQIIKDPQFTPIYNQEIFQYLLSQEQKYLVSNNYMNEQQQPDLNARMRSILVDWLVDVHLKFKLRDETLYITSYLIDRFLNIQKTTRQQLQLVGVASLFIACKYEEIYPPDLKDFVYITDNAYTKQDVLDMEGQILQTLGFSITQPSSYCFLQRFGRIAGLDTKNLFLAQYLLELSIIDIKFMNYKPSFLTSAAIYLVHKIRKTPQSWNEEMQNTTGYNEQELRFCAKEMCLVLQSSDKSNLQAVRKKFAQPKYLEVSRIRVERQTKSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4J8</accession>
    <name>A0A8S1K4J8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cathepsin propeptide inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0090294</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4J8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035822863" description="Cathepsin propeptide inhibitor domain-containing protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="2">
        <location>
            <begin position="29"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8045268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="141" mass="17142" checksum="76FCECDCD5CE78A4" modified="2022-10-12" version="1">MKNIIAGTLTTLLIGLGFYQVNLDSASDFERWALKHGKIYYGDEKIYRQTIYYQNKQMIDQHNKRNDITYQMAENQFMTITNEEFIRLYLNTKSSEQLNNQDKVKRNNIHKSSEPINKENKKWPIDWRCQQMVILWFWYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K5S1</accession>
    <name>A0A8S1K5S1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0140142</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K5S1"/>
    <dbReference type="OMA" id="QICVVYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035939185" description="GOLD domain-containing protein" evidence="9">
        <location>
            <begin position="16"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="24"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8049743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="195" mass="22644" checksum="B1488A3B4AF676D3" modified="2022-10-12" version="1">MKLLYLISLIYMGHGLSVQISQKPTCFYVKSYTGDTYVTVNYQISGLDENKTEITIESENGDKLVKKQNTKEGALKQLLREKGTYYICFKSLSKGYKTVSFDFDMDGVDKEYAQSEQFSEMSKELSRTNRNFQTIYRNQNWISDRENAHQMLLEQTQQNVKWCASAKIGILLIIAITQICVVYYFFKDKDFSGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6L7</accession>
    <name>A0A8S1K6L7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0130031</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6L7"/>
    <dbReference type="OMA" id="WNQEEDE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047157">
        <property type="entry name" value="PHRF1/Atg35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12618">
        <property type="entry name" value="PHD AND RING FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12618:SF20">
        <property type="entry name" value="PHD AND RING FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="72"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8048676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="26601" checksum="899105866F5CD4CE" modified="2022-10-12" version="1">MSRRFNQIQNKQIDWSNEQQRKKRLILDSPDTKETVTILPKQKIPNSNTLPKHFISGQKKKARLKQQTQEECPICLMNLDDVQKVCEIDVCNHQICSTCIKEWAEKYKTQCPCCRAKFKFIYPIKDGKRENNPIKLNLNLPKWNQEEDEFYEDDYDDDYDDEERCQVCQFSHTPYLMLICDKCNDAFCHTFCDPAQLELKVPSSKWYCLDCRKSKFIYYKHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7C7</accession>
    <name>A0A8S1K7C7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0170169</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7C7"/>
    <dbReference type="OMA" id="QFNAQFL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001808">
        <property type="entry name" value="Rab GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8050661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="201" mass="23090" checksum="E0EEEB8868810144" modified="2022-10-12" version="1">MNKNTNTYDTLIKVVIVGNCNVGKSCILMRYSENYFTSQYYNTIGVDFKTRVIKIGNQDVKLQIWDTAGQERFKALTNNYYRDAHGVVIVYDVTERSTFDAVDSWIEDIDKFGRKTVQKLIVGNKADIPNKRKISKQEGQEKAKQFNAQFLETSAKTSENVDRLFISICEQVMNMKQQQPYNQNNNLNQQQQNQPNGGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K893</accession>
    <name>A0A8S1K893_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0180137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K893"/>
    <dbReference type="OMA" id="QQYSYFD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="26"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8051519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="294" mass="34814" checksum="27ECF14FF0218E6E" modified="2022-10-12" version="1">MSEKKIRKKYHDKIDFKLDLPDTNNKKEKARQSAKNTRQRKKAYIESLEQKVAQLTAQLNQNKKVESKPSLMQLMEQFLIDRKQQFDMLEKAYQEGNEASIKVITNSLRFKYGISGQGRLMAINKVFELLIDLLLPMHMRYLFWVASEDVDIFKRERQSGEFYSFDMSDSQINCLNSFGLDDDQILKIQNMQQIIQEQRSHYDNLIIEIYKIQQSIIKESEKCQTIVDEIWDVLEPKQAAGLLITLDKASLFYNNRVVLNNLERRKYILIVQFQHFKRGQKKKFKDSNNVLILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8B0</accession>
    <name>A0A8S1K8B0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spindle assembly abnormal protein 6 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0180157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8B0"/>
    <dbReference type="OMA" id="KHDSMES"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10142">
        <property type="entry name" value="HD_SAS6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032396">
        <property type="entry name" value="SAS-6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44281">
        <property type="entry name" value="SPINDLE ASSEMBLY ABNORMAL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44281:SF2">
        <property type="entry name" value="SPINDLE ASSEMBLY ABNORMAL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16531">
        <property type="entry name" value="SAS-6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spindle assembly abnormal protein 6 N-terminal" evidence="2">
        <location>
            <begin position="63"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="201"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="298"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8051539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="594" mass="70219" checksum="8782AE9130117369" modified="2022-10-12" version="1">MQDYRGNFKRIEFGVQQTPAKSTTNMNDFQSSQKLKYEQMSNRPQSVNISFVNAPITTVSKELFTLQIPVKVKQVTDDWNTQLTLKMIMQQSQQAHGQTLRIELTDDNNLQFLQILDLTEGEFLALKNEQSLHVDFATFPMKLADLLQLCINSQRDEKVNFYVCLETKNGESTLAVIENNEFKKLTHLSLRLRSATDDLIKAFLANKLAIERQDNEEQQKRNKKLTELLEEKHWELENLKSEIRKFTEDKDTVIQQLLLDEQKKFNDFREQSLSKETNFKRESENEKQFIIEKYDKIVYELQNKYTQLQQINQEIQDQKSQLIQQERDLKNKLAILQQENQQFQKENQEFRLQNKELDTLKFTQERQLIELRIQKEGFEKLIHDKEEYLNNKQQLVDNEKKQNSILEEQVANQKKQIEKLESRISVMSEEVNKGNQIIEKLENELSKQKDKIKLKNSVFLQQEQTVQQLQDANDANHKQLNELRRDKDSLEIRLKDQENMNLDLKNKLAESQKLLESNNQMIQYLNKCLNETKVQVPAQMPGISSIKSQTFTKYTSPIPLQEDRSFRNQSVTYQQNQNNMSQISNATISNKFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K934</accession>
    <name>A0A8S1K934_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8051073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K934"/>
    <dbReference type="OMA" id="EESQIYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="389" mass="45434" checksum="51FAB4A0CE3ED4C2" modified="2022-10-12" version="1">MSQIIQENNSQVSNQAQESDQEMKKQQYQFIQSENQVLRKGKKHTIHQFYKNQTDRYLETLKSKKSNDTSEIMNNSFQAPNNSEQDQEQNKDQQLFIRKNDRKQSTKQFYQKNVMKMLEKFAKKENGQTIDDNINSNQTNQNDINYSEKRNEESQIYMNNSLFQESIKLIELKKNLEIMQSKQSPLIQLTKDDILQLQNQQIKQDTTQQNINVFFNSNTNSKVSAMKGNRRNQSIQKSQPKVQFPFLEQNNLDQEINQNLILGQNEEIKLDLSKLNAINRMEKKKKKNETQSANSKRSIRSNWTNKTFKEKAEEVAVPAVGTAIVGSLGVMLAKNVLKGKQQLLGIIPALACSAYYYYKVKKQNEVEQEFEDEEDINSEEEQDSTRLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9I9</accession>
    <name>A0A8S1K9I9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0180076</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9I9"/>
    <dbReference type="OMA" id="ISTYERC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000123">
        <property type="entry name" value="Potassium channel KAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="487"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8051397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="829" mass="96858" checksum="9A58E6609EEFA124" modified="2022-10-12" version="1">MKNLGQKQQIQEIDEEKQQTWKQRQSLSLDSDKEQTDNQKIEQRPSSSSPSDQKTVKNKNQSGNRAINSGTKRSSKTPHDFQEEIIPQVRSILKQGTFISHASVPRNKGIRPSRLQSLQSLQQSIRKNRVYPEEIQDNSGSNQNDSIQEDSFFEETEENLVDDESRYRFDRRYAHLTYMIFPDDPVKTIWDILIIIALLCVCFVVPYEISFKNDDTEYSAVSYGFNIAIDILYGTDIIINLFSAYVDDQDDLIVDRKTIIKHYLTGWFILDIICIMPLEYLIDDNNTSGFQKFAKLPKAYKMLKLIKMSRILKFCIQKKKYGEMLNQFSNISQNIRVMIISLLSVILVSHLFSCFWYFIGTISSDTQTWIEQYVDNESNFERYIMSMYWVFQTMATTGYGDISATNSTEQMITIFIMIIGVIYFSVTIGSVSSLLTQLDSQNVKYKEKIDTLNEIVKHHKIDNTLYAKICKVLKQGYKNNQNEIVEFLNLLPQNLRTDLSQAMYKNVFLGIDLFKHKPLRFTAYIGPLLTIFRVPEGDVIYNEGDYASEIYFIREGSVSLCIKECDYHPFVTIDVGQYFGEIELIKETQRKYTAIAQNQCELLSLSKSNFYKIFFTEFREIGEELHENARRKKRDYEEQFTKTKAFYQGLEQQAEQQLNEQNKIKVGLEKFKRNLMFQAQTQKGILDKAIREAEQSTQNELKRRMKRLKTVLLQKGIIDNMDEKSPSRTNGEIQTRKFDKKNTFLPMDIIKQAQQLSSDDENNVKDQQQQVQNNHRINKKAMTTFLSPESRENQNDFLLSQDGMLNSQTLSSYDTNIQFLKNRKKQLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KA18</accession>
    <name>A0A8S1KA18_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="4">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0110414</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 4">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="3">
            <name>Cd(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48775"/>
        </cofactor>
        <text evidence="3">Binds 1 divalent cation per subunit. The enzyme is active with manganese, cobalt or cadmium ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-II DAHP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KA18"/>
    <dbReference type="OMA" id="DECTEKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002480">
        <property type="entry name" value="DAHP_synth_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21337:SF0">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21337">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01474">
        <property type="entry name" value="DAHP_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0104">Cadmium</keyword>
    <keyword evidence="3" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602480-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8047408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="381" mass="44697" checksum="D4577F0A9DDD7C7A" modified="2022-10-12" version="1">MNQDKEWSIDSWKKYQALQLPLYSDAQLYQDTLQKIKNLPGILNFEEINTFKNQMIRVSKGEKFVLQLGDCAEVFDECTEKHWKEKFSFYDRMGQILDAIVIGRICGQFAKPRSQLLEKDGTLNYRGDLINSLSRDERDPDPSRLLLGAHYAKKGLETLKQYEGSQIWVSHECLHLGYESAFVKQNQDKQYYLSNTHFPWIGDRTRLHDHAHSNFIKGLYNPVGIKIGQTINKIEFINVFKLINPKNEDGRAFAIIRLGKNKLDKLKDIIQWKQEEKLNISFFLDPMHGNNQDKSGRKIRKIEDIMYEIQQFFAILEQQNEIPAGLHLECTPYDVTECIENDEDINPKKYTTACDPRLNFRQTRKIIIFVNTLLKKLRNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCQ9</accession>
    <name>A0A8S1KCQ9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0200117</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCQ9"/>
    <dbReference type="OMA" id="NGFRWPF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000019">
        <property type="entry name" value="probable aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044638">
        <property type="entry name" value="ALDH7A1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521:SF7">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 12A1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="4">
        <location>
            <begin position="47"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8053019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="531" mass="59758" checksum="7CF983AC517D27F0" modified="2022-10-12" version="1">MNTKIQEKISNPNFIQNTFPLNLTDLSENNPYRAMNFINGIWKYASNYLEILDPLNGDKFILVPNNLTEEEINEYSDAAKQCPKTGLHNPLKNPERYNLYGEIFHRASFLLGLPQVEDYFVHLIQRVAPKSEIQARGEVKVTQRFLMNFSGDQCRFLTRSFNVSGDVQGQQSSGYRFPYGPVAIIAPFNFPLEIPALQMMGALLTGNRLTIKGDQRVSVVLEMFLRLLHLAGLPPTDCDLIHCDGPQMESLLKRTNFRLLQFTGSSGIAERLCNVMNGKIKIEDAGFDWKILGPDVIEFDYVAYQSDQDAYAFSGQKCSAQSILFAHKNWVQAGILDKIKLLAEQRNLKDLTICPILTWNNQRIQAHLDKLIAIQGAKVLFGGKPISEQHKIPECYGSYLPTAVYIPLQQIKDNFELVTTEVFGPFQVVTEYENEDHVIEILEYIPHNLTAGVVSNDIRFVQKILANTINGVTYAGIKARTTGAPQNHWFGPCGDPRGTGIGSPEAIIQTWTTHREIIMDIGTTPKTLTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KFR2</accession>
    <name>A0A8S1KFR2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0180200</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KFR2"/>
    <dbReference type="OMA" id="NEMQVAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.36.70:FF:000001">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000047">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8051582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="39808" checksum="DD0250F860EF1503" modified="2022-10-12" version="1">MSEEHPAVVIDNGSGQCKAGIAGDDAPRCCFPAVVGRPKHQGIMVGMDSKEAYVGDEAQAKRGVLALKYPIDNGIVNNWDDMERIWHHAFFNELRVTPEDHPALLTEAPMNPKANREKMTQILFETFNVPSFYVAIQAVLSLYASGRTTGIVVDSGDGVSHTVPIYEGYALPHAVLRIDLAGRACTQYLVNILNELGVSFTSSAEMEIVRDMKEKLCYVALDYEEEMKKYKESAANNRPYELPDGNVVVIQNQRFRCPELLFKPNFIGLEVSGIHELTFKSIMKCDIDVRKDLYGNVVMSQIFQFIYFQIKQLSQIIKELIQQYISIWIIYKIHNIFQCKNRSIFKIIIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHP4</accession>
    <name>A0A8S1KHP4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0210098</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHP4"/>
    <dbReference type="OMA" id="KQHLFRH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03763">
        <property type="entry name" value="proteasome_beta_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF4">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="4 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0888">Threonine protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8053821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="275" mass="30272" checksum="90FEE17DA046C4FA" modified="2022-10-12" version="1">MQYFDNYLQSKGGYDYTNAKRNELMKAQGFKEMPFTKTGTTIVGVLFDGGVVMAADTRATAGSIVADKNCEKLHTLAPNIWAAGAGTAADLHHQCAHFNAKLKLQRLNLNRQSRVNEVITKLTSKLFPYRGQIGVALIIGGVDCNGPQLASVSPHGNYVYHPFQSMGSGSLAALGILEAKFQDGLTKQQAIDLAIEAIEAGIFHDMGSGSNVDVVAITKEGVDYRRNIRQYNAKSHQKQIPYPFPINNTPALRKYEFNFEKQELTEVGQEMEIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KI14</accession>
    <name>A0A8S1KI14_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="9906" checksum="A1BFB266D3119D2C" modified="2022-10-12" version="1">MANYQIAPSQSFQFNSFNXFQGQEPIILIDYNKFIYFIQFLEIHLIIIIQHSKIPYSYIFSLFVYQNQFYFLYILFYIFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJ73</accession>
    <name>A0A8S1KJ73_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0220197</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJ73"/>
    <dbReference type="OMA" id="YFTEKCD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000683">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="103"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8054767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="490" mass="57446" checksum="932D0F6FBA31F34D" modified="2022-10-12" version="1">MQVIYQMICIRKHILKSKTYYLEVHDDQMILSEHKNMSNAKYVINYTWETRVMWKASDNEQLCSFGIYYNNRIKWFDSDHNSLNLLKIHLSGKVFFGNISIFYDSNQILGQGASSKVCLVKCKKSISQYAAKCISKKYLLQKKSNDRIYRLQQEIQILQRIDHPSFVKLQEIYQGENSYYIVTDYLEGDTLYNYIKSYPDDQLPSYKIREAIKIILTALNYLASKNIIHRDIKLENILLQKPNDITSLKLIDFGLAIYKQPFQKLTICGTPGYIAPEILKHTNKEEYFTEKCDIFSAGVIFYKLLTKKTLFRAQNTQEIMEQNKQCQINFQEFEFKIQKEALNLLKAMLDPNPNTRYSAQQCLDHPYFSCKLENINILQEILDKATGFSGITQIIDQKNTNSIGQESQAAQIKRCNPTSYKIRMELRKRTKSPRKYADFQFYYPSFCQMNSYESLSSKGSSNNVSQFENLINDKLDSVIEEDEKQQIHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KR16</accession>
    <name>A0A8S1KR16_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0260296</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KR16"/>
    <dbReference type="OMA" id="TFHQKND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="347" mass="40401" checksum="34CF689AA7E5ADAC" modified="2022-10-12" version="1">MSFDKLMLNRPNYTTRKLLQPFFVSASTQNFVQTPIMNKTENKDRPQNFIETQRKTFHQKNDSNKSQSISIKKQRVQDAYNSLSDFNDKKQIIASLKTLNSADERIQLIDKILERKDEMMIAQLKLKAVKLEPIFNVIRRKLRKQRLGSATPNQSKKGTIIMRQKTTEDLLQTLPDQTRDSKEKQDHSLNQQETIIEDQIIEKIQTKFTAGVAFSWLANRHETKQVQLDPDPTERQYLRRSTALQNGKNTINRLKQQVSKKVSENSDDLIRRGNFNAEQKKEKSDLSKIKAKVDSGLNKPQLNSLPKINIIKRQDTLKYVTSERKEISTLGEIQTLRSESKTRFQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVC4</accession>
    <name>A0A8S1KVC4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVC4"/>
    <dbReference type="OMA" id="FYEIFDP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="276" mass="32737" checksum="E3D00F1389F5F831" modified="2022-10-12" version="1">MSNRLVAIGQNSLIPIKATGKLGDEFFDKPEYEPNSMELLKKKERHQELRDKGYLIKDKLIEDVPDDIPFYEIFDPPNENYRKIFYPQDLKSKVAYNYHSPNFENNEQFLVDQEDIEQAEQAIKQQQEANMRKESKLQRLNQQFKKQKELEAEIERRQRQDQAIENVKRERISSKHPISSPSKNQPQKVSPHLPFKNYGLAIVGPKGKMSMQKDNKNNKVLSKKQQKIEDDDENQKKKTVTFAEEDEVKYIPKNWKSQSEELREIIKQNKKNKKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWX2</accession>
    <name>A0A8S1KWX2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0230219</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWX2"/>
    <dbReference type="OMA" id="IVCTIKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362:SF309">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8055514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="372" mass="43667" checksum="E3F83DFBEB79B656" modified="2022-10-12" version="1">MGADQSKFDYNYGQIIERDNLCEKRLQYDINAKDVFLQSLTSIPILKSFEEVDKITFCGSQNEIKAFFSYNQITLHRFVLTQEFTLKETQIVCIIKQILSNLNQNKVQIHPKEILLPEYIMLDPELFLQKSDVQRIRQGNIKHQDYYLAPEVLTGKCKSNITAQIFSLGLVCLFIMTKSTPFDFNIYDTTSFQQLNLQKYINKINTGIYSQLLKNVIIDMLKEDPKLRINEDQIICRLDEVLMFHSQKTLKFQESAILMSVNSSKIKLDLNEELKQSQLSYNYKERPSENQKCDKEKKLSKHTANNSPQTNHQNRQNKENIELEFITKKEKRTNNYSFSACSSRSHIRGMSIKPAKKQVYQDKHTSIKQRWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXN5</accession>
    <name>A0A8S1KXN5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0280242</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXN5"/>
    <dbReference type="OMA" id="NPWFTYA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF47">
        <property type="entry name" value="TRANSMEMBRANE BAX INHIBITOR MOTIF CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8059121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="300" mass="34376" checksum="5B035E5768564EC6" modified="2022-10-12" version="1">MSENQQDDQISPLDFGKYHTEPQRDHREIKYMPTKLSEPRGSQQYPQHVGVVIEDDTTSNNDSRWYAKNNQYKLLIGNERTGFVKKVYSIMIIQLLITMIMCVISYFSIEYRMFQLQHSGYAYLALGIAIFIELLLFCIPKFAWRVPYNYILLMIFTICEGYMISNLCSYVFDKYSQNGGYIVLMAASLSLAAVLGLTFYACKTKSDFTTKGALLFMCTTSLLLFGIMAGVYYQNVINLIYSLICSLLFGAYLIYDTQLILGGSTHKLSIDDYIIGSMIIYIDIVYLFAHILIVLIACLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZQ5</accession>
    <name>A0A8S1KZQ5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZQ5"/>
    <dbReference type="OMA" id="INYKEYA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="40"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="76"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="214" mass="25898" checksum="D26ABE23EB318779" modified="2022-10-12" version="1">MQILPPADRVLHYQETKSWFNSRHSFKVKEKYRKTEKELFDDEVVTETFHLIDRDHSNTIEMDELFSMLKKNNYPVNLKLLKAFFEMTDRDGNKCIDLDEFKQVIKDEGTSQTFRTLMRKMREIGDENYYSTDFVNLLRYLSYCANRTDLIWQIKVFIHLYIYINNTEYTSIIRIKIQVSIRIIQSELIIYKDIKSQGGTNMYIETFYEKEELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1C6</accession>
    <name>A0A8S1L1C6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0310273</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1C6"/>
    <dbReference type="OMA" id="WTHPDRS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF565">
        <property type="entry name" value="AT28366P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="8">
        <location>
            <begin position="19"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8061367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="268" mass="30336" checksum="CF2B636A83363E11" modified="2022-10-12" version="1">MINSKVKSEFLQKLACCSEYAYREDTNYPIRNQMEDSHYYKDNCFEDGTACLFAVFDGHGGTDVVEYITKILPETFLRDFKQFNILKPNDYFEQIFKKVDDQLKLVGAAEIGATCCLTLLRKEDNKRKCYIANLGDTRAVMNIDGKAVRMTVDHKGIDPEEQARVKREGGTIVRGRVMGQLAVTRAFGDLDLKTVGVSVKPDLKVQEITPLCKYLIMASDGLWDVVDDQKAIDITKGLKNSDEMTKELLQFALKNGSRDNISILIVMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3C7</accession>
    <name>A0A8S1L3C7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIPF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0290321</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8060032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L3C7"/>
    <dbReference type="OMA" id="KKSTGYC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039765">
        <property type="entry name" value="Yip5/YIPF1/YIPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="68"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8060032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="252" mass="28782" checksum="DEDD822329559283" modified="2022-10-12" version="1">MEIGVQKQTQEEKQVQPEQFNKLDDSGDKKSTGYCGFLTVEYYTPYFNVTETDVIQRIQATFLPFRPDFLNITKDNPDLWGPIWINATLIFLITAIANLRQVDQEKENQSFDVGYVPQATALLYIIAFGTPAILAIVMKILGVDLSFFQTICLYGYSMSTLLPITILCYFQNELFLWLIIVYGVGNSILFLIFNLKEELDKLQIQKKYIIIGIVVIMQLSLYLFYKLVFFSYVSEGKVEAKVASSDSKISGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L441</accession>
    <name>A0A8S1L441_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0260022</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L441"/>
    <dbReference type="OMA" id="PDDEMFE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="634"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8057394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="685" mass="81765" checksum="30F81F98027D56BE" modified="2022-10-12" version="1">MQQQKEYIPIVLIDDKIKQQRIETEFTEEKLPLIESVIKTNKTSTHLLNSRTSSIKYIIPTGIKPIVFPNILQKTKEDMIKLRKYQLAQLHFDGDQHQNTTNLKTENRPKKTERDKSSQKMYTQVTKNSYNKPIITELTTLARDNYSASPRSDSFKFRSLSPEIESQLWQVNNKVNRINKAYEIQKEIHHLERFKTQWKTDVIQQNLSQKIVQVGLNTKKGLRPIFILIYLPHQNIHLRSSQTEVKSITNEVELSVFPYFVPVYEKIINEKEISENYKEWFSYFLDILHYRSYIPQAKTFTLDSIEISALNQLPLYEKFLYIQLQGQFDFWSIMKQKLNLSGEFQVDKYPKLATKLLDLRSKDEVSRFFCKRENLLPFYFRQVQMGSQTSIPSQYYIEIENNIREPDDEMFEQSIIQMNEKEQDTYLKLLQQYIHSNKSKYSKKLKNLEENMRLRLKKIKAMRRQSIQVRLKTKHFLRPEEEEHFERRLLYEDEKSIEEIQEDAYDHILPQNTHIFKKKSKQITSVEDPFQSFSAKDNQKFKEILQLINVEKIVLEKNLSRQDVYHYLSLFKALMDSDTPQRIKDVNYPTLFISLDQLRRGVPFIALYKNKVNTKKLAEVYSRKYNYCEFMEFLDIFTTDYKVTEQELQEVEQEIIYSQQKPNQGTSSVLQKQASVILGTENQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4R2</accession>
    <name>A0A8S1L4R2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C3HC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0290141</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4R2"/>
    <dbReference type="OMA" id="HRYFCKW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012935">
        <property type="entry name" value="NuBaID_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07967">
        <property type="entry name" value="zf-C3HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C3HC-type" evidence="5">
        <location>
            <begin position="7"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="648"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="731"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="233"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="435"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="654"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="686"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="731"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="750"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8059736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="762" mass="92077" checksum="5394ABD761F42B35" modified="2022-10-12" version="1">MYKQYFDEYCERLKTYNFLNWPYRDSKLKPSKMASIGYICQEDCLQCQYCQSKLIIDDQMLPNDVILERAINKHDQYCQGMAIKFKITQEFLHSYGMAYYNECLASWKDPIEVKKTYLDKVPQNLQYLLQNPKQVLAIFGWIKQDQILLCKYCGRKCQILEEEFDVFFEHRYFCKWANDGKNIKYGYLIVLQQLRDQSTQNDQDANDRDNNQIEQDQVRKTLVTQCENIISINLLTNKELQKAKAEIQTFQDQLVKRKRFDIKESEREMEEFELEIQKRVKIQKEQIEERNKEKINMINKQDSEEEEELDMAVQEQQIQNTNNKMNEQINNNSQQDNQIVNESDNKIEQEDNQIQEINKEQIENLENQNQIQEIKEQEKDENIQINQIQEESIQQQTFVQNNESNLGLIQQPNQTEQPLNQQQGDRLEQDIEINQEQKKNENIQSSEEIKELQEKKENVCINNTLHQQHNQNLDFEQHNNQQINENDNQIDEKQENQQMNICQDQIEQNEKQLQIEKVQQSQIQSQEIEIEQEKMESNNIVELQEVNKEIQKPFQDEKIKEFEIKNNIIQDDQMINEQQQVDIIDKEKKNNQQDDKNNIEFQVEEQSEKNLETLKNGLKLTAQNDDENLRNQEENKIMTIINLEDQLKSRQQEDQDIEQEQEQEQEQQQQQQEQQEQEQEQEQEQLKQQQDQQQQEQQEQQQQQQQQQEQQQEQQQQEQQQQEQQQQEQQQQQEQEQQEQQDNQNQIDIKNNENVDQDQSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5V0</accession>
    <name>A0A8S1L5V0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0340007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8062819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5V0"/>
    <dbReference type="OMA" id="ALKQSYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="192"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="273"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="123"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8062819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="431" mass="50654" checksum="53E7853BD06BECA2" modified="2022-10-12" version="1">MQQFQVSKNKQIANPTQLYNQFQVRSPFTNVENKSLVQKTASDMEMVNKPVAPTKQLTHQQTPNASEYKIQFKVGGGRQPQKDDSNQFNSQTTPFIPMSPQQRHENTQILQSPQNRQPYLLSPQHQLQSPQIQFQSPNNRDQKQQHYFSQVQGQQQPKQIAHSSSFVQPTLMQQNQQKIVNYTNTFALQEELKCLQQQSVKMTQEIQKLESEAQRVQSAQLIKELDDKIKLLNQMNKQLQLDNAELMKVPDVMALRDLIVKYQNDRKMAYNQLAVIKAEIQNYNVRCQDLETKIKIDTTQELNDLVQDLENKVQTLILENDRINLQLKNSNNVVNTIQKQKHENDLVKEKLIKSRKEEEALKQSYQKEEMKLKLYEECNDKLKLLQDENKRLQQILNESELTQNDLNSLQEKIQVIKQDNERMMKQLKNRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LA62</accession>
    <name>A0A8S1LA62_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0340132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LA62"/>
    <dbReference type="OMA" id="HEYDDPI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818">
        <property type="entry name" value="DOMAIN-CONTAINING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="101"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="280" mass="33174" checksum="142E1D8C0AC568AA" modified="2022-10-12" version="1">MDQSYPLIKPNQEILNYEPTADEKKTKIGKYTIRRLFEGELDEQDNAAINKFLEEAGPFQNPIWTRPQILRFLQANNFKKDQTVQTMQQYEEWVKTLPINYDQQVEQFLKAGIIYIQGRDHSYRPIIVLNAYKVNFNEMSLEQYLRGLTYFLQVVVNDMMVPGKVENWVILIDLDYKGMIGLQINALKQVMSYLQNNYRSRLYRMFIFNTTMMLNVTWNMAKLFLEEITQQKIIFVKGDVKQLFQSVNKEQIEEKFGGTQTNRVEFWPPSIISANFRKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCF0</accession>
    <name>A0A8S1LCF0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0370161</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCF0"/>
    <dbReference type="OMA" id="RNCIEAE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF11">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M14" evidence="4">
        <location>
            <begin position="263"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8065227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="803" mass="94373" checksum="7FC02311D66291BC" modified="2022-10-12" version="1">MQFYDFLPAPNPLIQATDPEMNTKITQIKKRDEYNERDSSKNLWDYFTDNKQNLVYQRYDNYPWSMIPLNNHRFTASCFFDCRKLISLNREIKLTEFQSVLYKNLFPSTMKQKEFKPLQLEQENKNYSGLKFDSHYESSNLFASFRIGHNEYDLIMQNDTNSKGHTQWFYFSVENTQKNALVTFNIINFIKNESLFNLGQRPVIYSIKSNKTRGIGWIKAGTNVVYFKNKYKRENSTIKTYYTLSFSYKFEYSNDKVYFAQCYPYTYSQLNSFIDRVIKTNQFSTVKDLCKTITKSQCPLITIGKGKKAILLMARQHPGETPSSYAIEGAMEFLIRNCIEAEMLRNHFTFYIIPMINPDGVIFGNYRCNLSGTDLNRIWVNPHKEIHDSVWYIRDLIKQINQSTELSMIIDFHGHSKKFNSFFYANNGADQLKLFPLICSKISKMVNLKDCSFSIHESKKKTARAALQEEVKSGYIYTLESSFHAYKRQFSQDFNDIHYKELGVDIILSIFKLFQQEMFQSTFEQNINFKLPNTINSQQNNDLDIFIKNYDLSQLQLNQDDSGSDSNPEEDVLQDQEQLSIIESRKQSKAQLITKKKIIKIDKSTQTDISHYQNLMLDEKVLSKIEEQVKALRKNNNLETIGILGNDVEFQEGQKCEFQQTDISLQQILGKMQVFHVRVSNKNSMFQHQFTTQSSKPVVIVLKNPYSKQQLQQNQLTTLQQEQLSIKNRRRVASINGRASIESKRLANERKYSIQHYQQEKYHGQSYQYNTPMNFMSQDTTFDKIANISKIRFNEKQVVKNQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCY9</accession>
    <name>A0A8S1LCY9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="TENYAME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1100"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1300"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1408"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1615"/>
            <end position="1642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1681" mass="200539" checksum="5FEAEE38F35AD6C4" modified="2022-10-12" version="1">MRKANNKKSNKGIQIEDIFSKMIQATMHSERSVRINSQIQLMLFFICKFQLIFFLFYLDYKWQDTNNLYLYDYILLVIFRIDLFISTFENISSLSVYTILSIIIIKYLSYILLSFDDKLSKNKFTYLIKSIGLYGQLFYTVFHILFSTLSCQYIYQQYKQVELNNLNYIHLLFGILCFVILQLEGIYHLQISEQSIDGKIICFDRLRITIKEYIIQFLNLIIIILFSVVKNSQIVSWIIHLLVLLSSSLNTLNLQENQTIIDNNKKFIIYFSDSFNILYVFYSLIQTIISTQQFQVFIYTIFSFPLLLKIFIKIDQYLNFTIFHQAFERKKIQLFAITQLLIKKQNNYNFPLIYKLFIYNHHKKKCNDIQCYCHHNKLLIDPKDTATITSEIDKDFVQKKLKQIRKFIINYKFDNLDDLQYYTFLYGALLSNNGWSIQSIRHFNQLLYGRKNLRNSQQSINQSILKPNSFSATQKNDSSSQTINEPEKKFQEIQMNEQKRHQKNTLTQMVKFDFIQLQKIEFFLTQTKHQLKNLFGGHWLTAEQHNLSEQIQAFMKQELKMDQYINEIRKILKLKIKYLENLTQQIKDKKISEFMKALTLISSKLIIFKNILQLEYENYQSKRLQSLQIFYEAEIFRNILEAFKLYNQASLSDDPISILNQNMNIKFYSNKISYIMVEIDDDLQGAEILKYSTNLRQLIECNPNDEIHFYSLFLPFISREHPLLISRFFKFGQSKFYKQFNQTFIKSKKNLAKSIIFSFDNLVQIEQEKITLIGLLQEIDIQNPFIMVDVNLIVGGITKSMFSTLGYNQIYIDNIADFSIFYQIKITQIFPDFDEFIQNENQSIIKYNNIDTYFIDLEYFFSELQTENFDSNHQQAQFLLKLWDNPHIKSYYSSITIYTYEIYGYHYYVIQIDSLVQTQKCNKTQEKNSEVHRNLEQSQSFEISQLNLSIEEAMPRLINQFTIKNRENYSQNLQKNSQIINLFAVQEVDQEEAYLKMDQLQLMLSPNKSTSQLIEKQQNKLLRQSSQQDYYTVNTNQKQNKQINSIDHEEFNQKGDQKLIENLLKNSLQSTEIGANSEVIKKYELLNSLTKIKIPKILKISLATFILFVLIQTICLSLVISILHNDIQQFISDIEIIALHASIQGPHDLFFSMRNTITSYQQMSREGFIPANKVKNLTEPYYLNIQYGYYELRDSFYQQLENDYLKMFLDEEVMTLLFMRNNDTENYAMEHLSFREGLLIILQYQFAQMRTFQKRESTAGQPYQVFLFSNYYNIQDKLQSITNDILQYSKNRSQNVGIKWQIICLFFAFIVIINTIFLMIELHQYYKLYDRFLQLLNFMDKQKISLEIDKLNQLYKLITTNQESIYSYQFDLQRQEQLMNNYIALNKITNTKKNVESNSQIKIQRRNILVFLILVACIFQVYIIVIQIKTNEYISKYDDTADFYFMIQNLKFRSGSMYMYREHLLRWKNFTYLTAYDLERAYQLIDKAQMNIQEYLDFTSSFQSNKYLLSDEFISFFEYQQTHDLCEFIDQIYIEFMSLYCEKSFDGLLKSGSISVLNFMSNQIKNQQAVNNFTKRAEVNLYEMEGSQIVIRSFFRVSDEFQIGLKQITTQQNEFTLIITIIFISYLIIFLMVVVFIIKNLLVKEYAMLRRVVYLIPQQIILGDESFERFLKQLALTQELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LI42</accession>
    <name>A0A8S1LI42_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0410196</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LI42"/>
    <dbReference type="OMA" id="IHHVINI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000128">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF511">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="17"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="78"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8067848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="236" mass="27570" checksum="FC53FAC2EB318184" modified="2022-10-12" version="1">MIQKLNKWDCIIDPIIKNNQQIGGIYLADIDCALSQELLQSLQVGALLSVIDEPKVTTSPNIIHQVINIPDCTEQNIQEFFPKTNEFIERHRQHTNVMVHCFAGISRSASVVIAYIMQKFNWGFQRTLNSVIARRPQVKPNSGFIKQLIQYETQNRNRSSSQQHRILNTINTVIDKMPYQSDSKNNKYFQPQKFDINQLEMEITKRQQDLQILRMKYQQLNDKIVNRNNVYVSAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LMK1</accession>
    <name>A0A8S1LMK1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0410040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LMK1"/>
    <dbReference type="OMA" id="ICNMVWN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="172" mass="20564" checksum="A5E09C6FE4F1F08E" modified="2022-10-12" version="1">MFIPDQRNLGGRQIICNMVWNHRLSLNIMDDKSAASPIQTKMHTPRQGCYTDRQGQNKPSFFFRRIPTSNYQKYKELLYKKPLSFPKNQHKKKYDTDIKIIDWNFVEKEINKYGKEFYKVNLHQFIQSFAKKMNIKKEYDFATLIEMVLNSYPNIKQEDLIEIASDIIVDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQI7</accession>
    <name>A0A8S1LQI7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0450022</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQI7"/>
    <dbReference type="OMA" id="PNQIWDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696:SF3">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_98, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="156"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="91"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8070027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="203" mass="24023" checksum="88C7EFFE08CEAEB1" modified="2022-10-12" version="1">MSEQDDEMLVEEILCCQNDDDIRSLVDSQCLDIIGNSLQDQLILAHKQLFRVIRLFREFLDSSPNQIWDQKKKKEHSKALQQDYLIINYELQREKSKVKQIEEANSQLKLQMKLYEGDFETIRQQSFEDIKKVEDQLVKTKNQISLYKNSLIQKFCVICLQKEYCIVLKPCGHVCVCEECSKRIDHCPIDRVKVLKMKKVYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LU77</accession>
    <name>A0A8S1LU77_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0430248</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LU77"/>
    <dbReference type="OMA" id="EYQPQKH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8069201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="14778" checksum="0ABB31A3CECCD5AC" modified="2022-10-12" version="1">MGSGQCCKSVERIETLEIDNSPPQNIIHSSPFDEAQQMVSLSDSEEEYQPQKHQFGVFKERKKTSQLSTGPQYSFQTIPSEQQFSNFITFQAIPSQQGIHNNLFSKFQKEMNQFAKKETKNQKNLQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LW14</accession>
    <name>A0A8S1LW14_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0450284</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LW14"/>
    <dbReference type="OMA" id="RIVCKVR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23143">
        <property type="entry name" value="RING-HC_CeBARD1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF22">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="545"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="974"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="1048"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="715"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8070482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1212" mass="144418" checksum="7E2576ADE8F03DA4" modified="2022-10-12" version="1">MQQKQQKLQQQEQKKSELYIGKFFCQFQCNGKMTGKNQLKVKLKIFDQKKTIKQLKKKKLYVVSNDIDQYEYNRIVCKVRKGKEIVKKIYNNHGKILHLYDEIWFFLLNAGYVKLRVKIIKLNESDVEIAEVSVYLTQQAIDDDYEMKINRYNKKTEQLNNVEENQMELLKYARLSLLLMFDFMNVTVEENCLIKQNKQFKIFFAQYCQFFQKSKMNFQVKSLLKPYARPIIEKQQVFENYLNSKRFNNNLFEKKLIQDLVVYQTGPSTFVSQYKKLYSPKMEEIYPIHHDLFQKIGLEFFQYVKAFIKRSKNSVDNQYAEIYDDYVLRTVFLCTCEISEKIQKDSIYTNQHKNLIKVRKQITEALSRSNKDQLNLLSDKNLKIYQYTKNTIHDLYFIDFKINRRSIQQYQQVFSQFFQPRFYQQYNNQGYSEMEKYIQKQIYEEIKEQIKYQKTNLEQNKQDINDIFLINNLILKEYNTPKLMISELKKHQKEALFWMLYREGHINDDQLQQKQQLSPLWQEYKLQGGESLYVNMFTGKVSKEKVTIQETKGGILADEMGLGKTLMALALILETLKSEQQTLIVVPKSVIKQWEKEIFKHSKPESLKVLVYYKKKDRKNKKTDFKNFNIILTTYAVLSIDFQIWNQINNINIEQDGQIDESDSFVERVQSIEEYSELSNTMDIYDSSSDDDDNQQSQFYYNPFYEYQNEKRKQERQLKQQLKQKQKEEKQEKQKKKRQLTPQQLEIAKEHNNLFKQKYHRVILDEAHNIKFRQTLQSKSANSLEADFRWCLTGTPMQNKHDDLFSLLQFLRVETFSEYFWWNTYINKEENEEDQQRILSQILQPIILRRTKNSQRFDGLNQVDEEICWVELNEKEQILYQKLLAGSQDIFKHFTIDKNNKSYVHIFQIINKLKLACNHPQLALKEINLDKTPIEEVIDKINQFFSNKQKSTNMTEVYKKSLVENIRNGDVQECEICINTQIDTFCLSSCGHIFCKKCFTQAINQQQICPVCRTTLSITDLIEIKVENENDFEDLKTLKFGLSSKLVSILNKTKIVQQQKEKVLIFTQSIDMIQLIDNLFQDNGIIAYRITGQMSVDKREKIIKQFKDSQDAIALLLSLRATSTGLNLTMANNVFLVDPWWNPAIEDQAIGRADRIGQLNQVKVVRFLCRNTIEQSINLLHQKKRFQIKRAFSGEAKKAQELQDFKFVLFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M335</accession>
    <name>A0A8S1M335_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DAAF9 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0520221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M335"/>
    <dbReference type="OMA" id="EYTANHN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22936">
        <property type="entry name" value="shulin_C20orf194-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056498">
        <property type="entry name" value="DAAF9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040342">
        <property type="entry name" value="DNAAF9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33664:SF1">
        <property type="entry name" value="DYNEIN AXONEMAL ASSEMBLY FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33664">
        <property type="entry name" value="RCG26366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23281">
        <property type="entry name" value="DAAF9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAAF9 N-terminal" evidence="1">
        <location>
            <begin position="13"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1119" mass="131143" checksum="10190198DFFCE7CC" modified="2022-10-12" version="1">MLQFFQRLENPDSKFHLYDSFYRIKKIEKVCIKENVDGLLIINGIDSKDNYEYTKLMNWVFLGHSGIEIETNEYLDNTLSETIFIITKDHMRAYCDQQGFKQLKPCLLQMPNLKLFVPTIKEDENKDESEVLKMAQFVKMMQGLKRIGVLLPQEKNRNIEKWPIIQGYGLLGNGFFSMQHEIVNMTERINQLYKPHDKHSMKNLINVISQRLNGHVSGSLQLLSEIPIKKRPAVTNEQMVEIFLDTFEIEDNKYVKRNNKLIPTVTFGNCSSQEKLPLHLTMESSDLYSSLRFSRTYFLTKQVCRENVIIPIHDDFEHFVKEYQYQIVEHSYHKDECLLIEIYKTLIESFYSALQMICQDYRKMTNQLIFELCQERLNPTLTNYQLQFLNDNLIVSVSSYNSFGEEVPINFKDPISFKLTPQFFSIRLEYKSIPSQVVKGTILGNVIFSDSFILQENCYYIITSKIPHLQIINTDARFSDDIEKVKKKLPLEKLGQQISEKVDKNRLFIPSKHALLDIPIQIASYILHENGIRIFTNEMGWFFILFDQMKKIKFTQGLESVWMIFMTEPILCASLQAKTIALEFVGKSVDQVYMKLINILKENEIKVDYTDNPKEFQLTRLGQMILKKEQKVPLLGNMIRESSFLLDYLESKQLIILTQVKSDLKLLNYLALQDESKQIDDRIGIILITGTYSSGKTKFAQSLQRYASDFNQKAQILSFSLLEQSILDEEGWLNKLENQLKYSQEQCICVIPFYLSIDIIVQQLSNGKLSKFVYIKNVVNKININNIYQNENCQPIDNLVSQSLPGYCQFTILDSFGNEESEVEEYFKIFRTVCIKSNIYRITNNMIQSSVAKEIYQCTSYNSTQNVFLRSKLASYKSQIVPQQKVFIHNTIPCVKQFRKILRELFKTSQQQKIQTGNYEKLSEIDKFIYRVKEFNSPQKPQLQYLKAFLRFEGQLDKLYEYTANHNYIVERQVKSVNILLKEQLINGTKLQIPSYVGWDTHSNLGFFMVGQNISKDICLEYIGELIQKMKPLQPLLTKNNLSKDTIKDIEFQNRIIGLENGEFYDGQFIRNQDGEILEHHPKLEIFIQEYLNIQNQKIVEQNNQIQQEWNNYLNALKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4P1</accession>
    <name>A0A8S1M4P1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4P1"/>
    <dbReference type="OMA" id="VFCPPMM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="547"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="587" mass="67313" checksum="FEFAFF276BB0D6B6" modified="2022-10-12" version="1">MSDNLQVKYRRRNSPQEGEQDFTQRQENKYNKIQRNDLQAYVQQPKIKRNRDDQIEDPQEDDDENQSDKFGGFVFTKTKNEPKKQYIYEPKKQQPSQQEKKEYQPKKDQQPKKKPEKKSNKFQQQSQQQKEISIQQLGELEKLQQHLINCGISITQDQENSHSDLVLPPDFNKPLSIPSKPIVAPPPGLVDKDKSSDFVDILNQLQDNDSDGQMNYDQHFSSSVLTRGAFPIQTQTSNQFNNQKPIPNNNQNFPNNSNDQKNKEKKKKNKKKKPKNNKNQLDMPMLNNQPDFMSQNSAEIFDPFKVAPFSFPQQQMGRNNLPDPLNISGPTFTTPLGFAPPGMMGLGPQIAPFNSNLLGAPQSLGFPPGPSLGMDPLEPTFPMGPNLGPMRMNQMMPPKYNQMPNMAQMPMPMQMAPPPQMKKQSNKNHMMMQPPPNQFDNQVFCPPMMPPSMSNHFSDFAPTMPMGQQFPPNLMNSGPILGMPPMMPFSDDFDQNWMPNDGMIQMPNQMQVFSNQNFQNLSQIQHPDSYMQESENYMSQTKKMNDIKNGNGNNIDQINQQCQNTDGQPKQRKKLNLNSKPFYPVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5T3</accession>
    <name>A0A8S1M5T3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0480146</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5T3"/>
    <dbReference type="OMA" id="ETKQNWH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00878">
        <property type="entry name" value="Arf_Arl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001166">
        <property type="entry name" value="ADP-ribosylation factor D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="127"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8071976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20569" checksum="4AA61C6D1A10BF63" modified="2022-10-12" version="1">MGSLFTKLYSSLFNKKQIRLIMIGLDAVGKTTILYRLNLNQEFKAPIPTIGFNVEKINFQNIQFTCFDIGGGYKIRLLWKQYIEEGTGIIFIIDSSDPNRLPEAKQELMRFLNENETKGAPLLVLANKQDIAKFTVDELKQFLDLPYQNRQCYIQPCCAKTGEGLQEGLSWLTRIYNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M645</accession>
    <name>A0A8S1M645_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0520168</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M645"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045862">
        <property type="term" value="P:positive regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="61"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="8">
        <location>
            <begin position="247"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8074262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="331" mass="38875" checksum="E631DF3C33D114F6" modified="2022-10-12" version="1">MGNTAPNYEEISDILSEQEYQQKNLSQNYEYPERKVLKAAKKSNEFNGIMNRRQSQNIKKSLEYKTQGNLYIQQKQYKKAIESYTQAINLFDRDSIYFSNRSVANKLLNRFQEAKQDAQQALKIDQKNSRAHFIYGTIILIEVQMFPDTTESLINQAQQGIKELESAQELVKISKKEQKNKLSVLINQNLAKGKRMMYLIRQEIDKKNIQTLKQTLLDISHRQQQKLDWSAIEQNIQKQRDIQMPEYFVCPILFEMIEEPILLNSGLTYDKSSIQQQFKQNGYIDPITRELIDPLGLIENIQLAIGISQIKREYGWIGIENEKDYKAIRFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M6L8</accession>
    <name>A0A8S1M6L8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0550053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M6L8"/>
    <dbReference type="OMA" id="YCQIGNN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="336" mass="40034" checksum="6C711F5C2E6D874E" modified="2022-10-12" version="1">MNSQISKTYNKISDVFSRTQIKQAVEGLDTCRQRIKLIDHIIDRREQIAKSEDIAKLQQYAHIFKKMLNKYKKKKSDQEQQKKQQKLDLVMQLLSGEKNKKQEIKKESRVILKYCQIGNNKLHPIINKRVVTPSTKADDTKIQENTQVKSRKISRFFTERDPLQIEAQIEREVCVFLEKNKELNYLQKERFLRLTDDNKKFQYLENQIKKQLKQQEYQQQQTAVKQYYRVQNLFHKNYFKGASIKIIKTQKTQPMTIAKDHCKTERQQIQDQESFASYTEFKLNQLYTDSIDAQRSLKSKHQSCPNSLLQSYLISTNVDIIDVSNQKLFQIKKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9Y3</accession>
    <name>A0A8S1M9Y3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0570156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9Y3"/>
    <dbReference type="OMA" id="MYAGIVH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007246">
        <property type="entry name" value="Gaa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304:SF0">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04114">
        <property type="entry name" value="Gaa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="514"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="544"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="585"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="607" mass="71742" checksum="23EFC7FE4E2F2A6D" modified="2022-10-12" version="1">MFKDLGYQLLIKWKLWKVLVVIFPFTFIYGIYLLFVVSSNSDRNSFDGSQLIHVEINNQLAQTLSLAAEQYMKEFGKTRDINQLMMEKLSNLGLETFYQEKYKNIIAKLKAPRSPDYECSLISFEYNDEYPNIKIRSIAYVLALIKLYQSDQINYLSRDIIFVGYHTKYKRYGQGITAFLEEYVNPLGVSFMPRSGTIRSSININIDDKFDSIALKVFGLNGKVSDRDYYNSINMIMEKQNFQYQFTESNYSWMQQLEKYLEKEIRSYWKLLQLDSIYQIPSKRFLFMDPYFSNQIEMFQGDLHDAHSYIMKYGIYSLTIRGYNTQNKDDSVLLTNKLLLVGEASIKALMANDEFIHSGSTLYIPLNRSFTLTIQNYCLPYVFVILSCAIFAIKSVYSRWHFNLFNDDKKELGRSNSMVAHKAIIEIFMGFTLCFIPNILNYLDNKQFDYFDQYFAIMLTIIVIYLIIIYSSRKWIFGQFSLPRRESVAEWLWVELVIQVCFVMMYAGIVHIPIATFIAIFLGPWFFFIQPLRFKPNFKIWDIFSYVINLLFLYGYFQILSYFGFDQMLAFYKQVVINYNEVGVYLFYYLNGALIPSLIRFAELLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAM2</accession>
    <name>A0A8S1MAM2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAM2"/>
    <dbReference type="OMA" id="KGWISND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="598" mass="70354" checksum="F646CC7C75BB1512" modified="2022-10-12" version="1">MKMVNETFQLEQIKFKDATPAMKNRIGLRCKTLNQELEDLKNKKFDKICSEFGCGPDLTQKFLYQEFQEKPFKEKAKELLVRHKEYLNLTQYLSTHMKEEKRELIEYLIGKLEKSKAIQNDLKEGKNIDFSKFFISEFKEITEDQFLGIPKIEYQAKIQSMLDQVNQALKEEQLYYKVASLAKPIIQVIPGTKQMQKVNPADICKQYFARYEELQKFLSNQKNLSWTPLPIFKLESENREVSTINKDVPNGILRIQFKNFVNGDKLLLQYELNIGNQPYQGKTDQADSQGRLGYAQDINLKDFKNGTKEIHLSVIEIKAFKKGWISNDLKGQGKISLNDLLDMSTIEGDLLLNEKTVLQYCIMLRESLNKKKTVTTAQILKTYPKFDKTNGMDQYLEQKLKEIPNEPVPQQQQKPDIQQQQQQQQQQQQQQQQQKEEEEACQGPQLQPDQLILLNHQENDEEFQNVKKFGEKMPQLQEELLNPENPLRGSVFYFLVQYSPFLQQKSVELKKDPSEKLQQRFVQKLWEDCLRQQNKLKNKLMNDMESYFNDLQKIIKKDQLMIKLYKQAQAPTWAKFVEGRLEIALQEEKAIQEQLQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCN3</accession>
    <name>A0A8S1MCN3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0590103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCN3"/>
    <dbReference type="OMA" id="HTRIMKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8077977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="103" mass="12437" checksum="9DA49EA5972FACBF" modified="2022-10-12" version="1">MEKKSQKQLSLDTSYDDYNQRDDEDCSEDDRGKSKRMDFRSKQTKKLEKQLIKLDKVQNSQQPVSMEIYYLLKFQQNLIYQVTSLHTRIMKIIENQFDYSTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDH7</accession>
    <name>A0A8S1MDH7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0510012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDH7"/>
    <dbReference type="OMA" id="EDYVQCT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8073394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="172" mass="20790" checksum="0CA0DBD2AD3090A6" modified="2022-10-12" version="1">MNRQRKLTNFEEFRQDYFDVIADDRAKLFNFFFEYNRQFGFINQEDYVQCTAAYLNYRARTSLALFGSPLVVFIANKTIFRNFQRIPVFRPAVFSIKYIGIPLLSFYVTSKYFCQDTEKLFYEMAEKYQFGFYQYNQAMDLLERAHKANRLDEFMEKGTSFDWTGIPELKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJ38</accession>
    <name>A0A8S1MJ38_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0620046</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJ38"/>
    <dbReference type="OMA" id="TICKCEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8079479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="321" mass="36879" checksum="A93D9275EC0C5772" modified="2022-10-12" version="1">MGDKKKKGDQVEDFSTEQLNKLYRKRCEINGVPLCKIFKERLEAVCSEGEHLQNVKLWEEMGPVGVRAMMESFTEIGYKHLKQLRLWRVKCQDEGVRTICIYIDKVRLLEVLDLLDNQIGVLGCKFLADILHPKCENKLVKLKLDHNQIGSEGLAELAKGLAMNNTLESLALNYCGIEADGARYLQDILANVNSKLYKLKLCGNRLKNEGAYELFRALEINNTLERIKLADNQFHSDPSDQTLINKILSTFELNKTLGYYDLKFNLLSDEDATRIIPLIEKNKSIFFIEISDQISKPLTDKLKSLTKKRKPKKKKKSKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJ69</accession>
    <name>A0A8S1MJ69_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0620066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJ69"/>
    <dbReference type="OMA" id="FANNHET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="21430" checksum="6473B026F69B4111" modified="2022-10-12" version="1">MSRRDQIEKDKQALLSKPWVKNHPIFTYDHIAEFYEMFILYAEPRTKKADVRDILVTAKTLGLTDKFPIIAHALDDLASSYDDAVDFETFISDLTAKLGNPFDQKGRVQLFKLIDVDGKGTLDKGDLQKISEELRFNLNEEDIQEIIHNVAGYEAEDVSEEKFEKYLGKRVQRRQVEQEIYRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLV4</accession>
    <name>A0A8S1MLV4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="3">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0590226</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8078223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLV4"/>
    <dbReference type="OMA" id="RDSYCRT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11610:FF:000017">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000119">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="9">
        <location>
            <begin position="614"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8078223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="984" mass="113546" checksum="D53C3EF80C61A673" modified="2022-10-12" version="1">MLRFVARNLNNVYKFSELQKFGVSFLGSGNAEYLENLFDQWYQDNKSVPATWDSYFRQVLESNNFDFTPEPQNGQAVSVRLDGQSGVRKLLSDHFRVLLLINKYRHRGHEKSQVDPLDLEHIQQIGKVKGYTKLDYSEYFTQEDLDREFYIHAVGSTGITKDKQMMKLRDLINYLEKAYCGKISYEYMHIQSNEERDWFRDQIEKYDEFMPSKEQKLKTFERLGQEHAFSTFLQKKFNTSKRFGIEGCDSMISGLQSLIDAAANAGAEHVVFGMAHRGRLNTLYNVFQKSAEEIMVEFQDLKSTFNEDIWGNSGDVKYHLGSVHNVLFGDKKLRLEMLPNPSHLETVDPCVYGKVRAIQDYFQDSKREKAFGVLIHGDAAVSGQGIVYESLQMADLEGYKSGGIIHVVSNNQIGFTTVPKDSRSGLYCTDIAHAISAPVIHVNADEPELVDKVFKIATEYRYKFKRDIFIDLVGYRRYGHNEQDQPKFTQPIMYEKIDKTPPVFIKYSDKLIAQGIVTKEQVDNLMKTHEDNLELAYQKSRKMDYNLKDWQPVPWEMIKVPVLWGRIKDTGVPLNVLKTLGEKINKIPNEFNAHPQIRKFYEERLSWIQKDQPIDFAAAEAMAFGTLLHEGFNIRLSGEDVQRATFSHRHAVIHDQKDPNGQDYVPLHSVIPKGQEHRLSIYNSHLSEYGVLGFDYGYSITNPNTLVLWEGQFGDFANGAQIIIDNYIASAESKWDVDSGLVMLLPNGMDGQGPEHSSGRVERFLQLSDDDPAVFEKNLGVRLTRQMRNSNMQIVQCTTPANYFHALRRQLRRDFRKPLIAMTSKRLLRLQAAKSKLIELTTQFNQVYDEAFPEFLVEPNQIKRVILCSGQVYYDLIKKREELKQNNVAILRIEQLAPFPYEFLQKIIGKYNKAEFVWVQEEHMNYGPWAFVRPRIQSVLAKTTNISNSPIQYIGRKPSGSPATGFHQLHEKEIQTLLQKAFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPG4</accession>
    <name>A0A8S1MPG4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0660004</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0660005</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPG4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8081459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="12875" checksum="2B6492CAB90E5FAB" modified="2022-10-12" version="1">MSQKYKKNLNSDVGPCLIGINFGRNFGSSFVNKRAKMMIVRINKKLKGLFESFKKLKQKLKWIVIDNDFKHFKFEYENCIIVGIWVKMKIDQYKIREGLKRQKRLIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQ43</accession>
    <name>A0A8S1MQ43_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0670190</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQ43"/>
    <dbReference type="OMA" id="KCHATHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969">
        <property type="entry name" value="RING ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="411"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8082310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="458" mass="54160" checksum="6E4ECAA71950B125" modified="2022-10-12" version="1">MLQKVILKTKIYSWILIICSGIVTLLACILAGSVNDQEVKIYGTLYIITALIFMGINCYILYRLQTINLINAEINNYNCFQSWKVLHDSILNNSDLWFPKYVLKCLVIIVFHSTLSFITRLKYHNDKFEFTQNISISIVLFVIIFIFGFLLQFYLVIQLIKMLADFKDYSREIVFTFHIKESLQEEKLYKRGITMIAIIFMLGSIGYYGIKLALYIMIAKNNEETNNKVFSIIVAILNVSCIILCSRIYRQLKSIYKQIPDIQDVRIYSLNIPCFTGWFILVRSLVFCKKFRFWRNLTFILLYIQTIYGLIKFPFYQIYNVTIVQMYSLESYLYCDTAIIVALMFWQFIEQFARVVHQIKRKYLAYQLEDRRQNIILPITIDLQINDQGRINNQQIIDNLVENAQEDYGECCFCLDKISKGQRVHKLKCHATHIFHYYCMSKWLANHNNCPLCQQIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MW05</accession>
    <name>A0A8S1MW05_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0720039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MW05"/>
    <dbReference type="OMA" id="NQYYELF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8084390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="22907" checksum="3CABD22FEED665E6" modified="2022-10-12" version="1">MFHLFEDSSSDSEEKAFQFEKQPIINKLGVQNIKVQDANQYYELFDATKKSTNEAKLLQKVKDLFLNKKEMDWYTETDLIQQQLSSSDEDYNNKMWNKKKYQKSKRYFDYKYYQFCWNCRQYGHEMKMCEVEKQLYCLICLSLNHSFYDCEQKLEKGNLNFDVQSPEAKCLNCLDLGHINCRFNNIKQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MW08</accession>
    <name>A0A8S1MW08_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0720140</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MW08"/>
    <dbReference type="OMA" id="RTNTENQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140575">
        <property type="term" value="F:transmembrane monodehydroascorbate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045150">
        <property type="entry name" value="CYB561D1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422:SF24">
        <property type="entry name" value="DOMON RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422">
        <property type="entry name" value="OS05G0565100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035796615" description="Cytochrome b5 heme-binding domain-containing protein" evidence="7">
        <location>
            <begin position="18"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="8">
        <location>
            <begin position="413"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8084548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="819" mass="97374" checksum="8D5ACAF9D19286B0" modified="2022-10-12" version="1">MKVLFLILPIIYACTDSFDCFSESSRFNCNGEPCLNNITELENFVKITKNLTMYFELDISNITMLFELQFCQRFAIVFDYTKINQLNKTYDEYDITGKEIIFFRWDQTYDNLYVNDNIGQSPTSFVYDEQKVGFQNWHILGYAIINETIRIKITRTLLIDNQNYYQFNKQTLDIGYLIVYNKEGIVYKNYNRGVITVDLYNLASYVEKYEQFTDQGHTIFLFIFWSFLSDIGIFFGRQLKSYPKYAKVHGMIFLFQSILTYIFAFSKIEYNKLKIQEIYYYDGLIIAHFIIGVLILSFLLLQLMMGMIVKNNLESLNGSNKIYKIKRIHFYLGYFIYLVTKAQVAMGVHIQRPDLLIPFYYFYVFLFFSKISIEILYHYEVSIIPRRRITYSYVELDKNQIYENLIDKINQNISRNQLVKEYQKLNYIILREAIYDVSDFNHPGGQYILERITGREIGRYFYGGYPIQNLNIKMHIHTQFAINYIETRYLGDYRNDNCPLLYYEENTSQIDKYVWKFEQYIPFASDVGLFIFKSHFFQVRQFLKGVQWFGRYFYIKPFGTGINLVERPYSIVMSYTQSAILKRQQILDYFKSQVIEQDYQAYIPKQQIILSNEINFVIKKCDFSNNMSRFLHCTGHKPYEITGPYGFGLELDPLSDGIHYAFVQGTGILPFLDLIDYLLRKAIYTILHDQRSKEIADCINDNNEEYLTTLGQDFQLIIHVAISDIAHFYGIPIIQDLLFICKTFHLDFFDIKILIKDRSNIPEELTTYMVESYFDLKYMKEHIKFKPTKVYICGSPQFEEEIQTNLFDLGIPKKYLVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MW32</accession>
    <name>A0A8S1MW32_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0720054</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MW32"/>
    <dbReference type="OMA" id="SERDYVM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="134"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="331"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="481"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="481"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="502"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8084420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="561" mass="64389" checksum="AC128545BA766788" modified="2022-10-12" version="1">MPRHSSSSSRSHRKKHSKKHRRHSSSSSSSSDSSSERHHSRRRRVHHSRRSRSPRPRSSSPIKKVFMNPIQSISSAETIKEYRAQHNIFIRSQHVNVPDPIMRFEDVQCFPQILMDLLLKAGFKGPTAIQAQGWSIALTGHDLIGIAQTGSGKTLAFLLPAIVHILAQARSHDAKCLILAPTRELTLQIYDQFQKFSVGSQLYAACLYGGQDRYIQKSQLRKGPQVLIACPGRLIDLLDQGCTTLKQVSFLVLDEADRMLDMGFEPQIRKIVDQIRPQRQTMLFSATWPKEVQKLALDFCKQEPVHIQIGNVELTSNKMIKQIVYVMKAIEKNQRLLYLLKDIAHKKILIFCSTKKGCDQLQKTLDREGIRCLALHGDKKQSERDYVMSHFRNGRSTALIATDVASRGLDIKDIEIVVNYDMPKVIEDYVHRIGRTGRAGAIGQSISFFASDEDARMAKDLVEILRESQNDIPYELRSLVDQNNKGNNYNPYRRWNGTGPRHIQQPFIQHQHQTQNQHQHQHQNQNAHQHQPQHQPSHYPSSWQFQTFNPQILQQINPYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MY03</accession>
    <name>A0A8S1MY03_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4485 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0670188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MY03"/>
    <dbReference type="OMA" id="DQFFQYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055310">
        <property type="entry name" value="CEP112"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027831">
        <property type="entry name" value="DUF4485"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18871">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 112 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18871:SF2">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 112 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14846">
        <property type="entry name" value="DUF4485"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4485" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="216" mass="26080" checksum="75D3AE80D7856AB6" modified="2022-10-12" version="1">MDEEFIKVMLSIEETYQNLNKHQKIRVEAWTKKLCQVTTNEVWKKNRNLYARILLNQVQKGILSEPFDKRPPEGPLPKLNRYLVLSDQKENARPQTNKKDSLFFEQQQQQQQSVECPNLQEKMLKLLIDCRQYLEKKTDHEAILLKEQQDQFFIYLQEVNQPYILEQIDEKTEENEDQQKDTMQFLQYLDNFEKKTEKLRVETQKLLHGNNIQYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MYR1</accession>
    <name>A0A8S1MYR1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MYR1"/>
    <dbReference type="OMA" id="MQQPDFS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15543" checksum="AA72324F8BA89A2D" modified="2022-10-12" version="1">MQQPDFSDCKEYSLEEMKNFQNEEDIQKLTQIQKELHLLKDVQNEIDLLVSKNEDPLDIISDQTQKSSTNVNNIKKSTEQALDDTLNLRSTKMTLAFSGVIGAFSMIKGFGLTIGAVVGGYIGRKAGQLSQGNIKEKASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0Y0</accession>
    <name>A0A8S1N0Y0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0760190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0Y0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="115" mass="13724" checksum="D24E5888A0A0402C" modified="2022-10-12" version="1">MHKFTLTFLNKDVKQQYQSMNKKQSIEFQNQQKWIFKSFLQIYASNKRHKLYDFFLIFVVVSYSLYFPLSKYFSLTSGDNQYVDGYLISVGTFGMINIFSFRFKTTILAQKISSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4I4</accession>
    <name>A0A8S1N4I4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0800170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4I4"/>
    <dbReference type="OMA" id="HDELNYG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.440:FF:000071">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.440:FF:000072">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002713">
        <property type="entry name" value="FF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039726">
        <property type="entry name" value="Prp40-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864">
        <property type="entry name" value="PRE-MRNA-PROCESSING PROTEIN PRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864:SF0">
        <property type="entry name" value="PRP40 PRE-MRNA PROCESSING FACTOR 40 HOMOLOG A (YEAST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01846">
        <property type="entry name" value="FF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00441">
        <property type="entry name" value="FF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51676">
        <property type="entry name" value="FF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="3">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="3">
        <location>
            <begin position="48"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="4">
        <location>
            <begin position="160"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="4">
        <location>
            <begin position="299"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="549"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="565"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="578"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="595"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="627"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8088267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="645" mass="77643" checksum="9D5AF6A0D4EF101B" modified="2022-10-12" version="1">MDSIEKEGDNERGYWSKHSSANGQTYYYNVKTGQSQWEKPECLQEEESEVEEEWQQYLTEDGKPYWYNRITRESKWQKPEEEQYTSGEEEDIIPPNPIDQFTQLLKDNKITSSVKWDSVVKQLQSDSRWKCIGSISHKKKIYNQYLEEMKKQEKEENKTKLSMAKEDFMKMLEEHKILSSDIKLWKVQSYLVTDARWKAIPDEKERENLFQDYLDKLYKQEQEQMKESRKTTTEDFRKRLQRHIEIGVLSHSSTWDECLKLFSQDRLLQQMLPIDALGVFEEVINPLYEQWRQSIISKYRQNRINFRELLQEHLAEGLLTHKTKWGQFVQTIQQDDRFICMLGQPGSQPHELFQDFISHLKQNHQLYKSELKMHLQQKGVKVLTNVSFEEVEAYFVDNQIWTKMPSHEKKYYYRYFQENIKQSSNIQSKRYKKMCKRYIKLLKSLPDYKSYEEMLPIINQKIQENPKLAGLVEEQKKAQYESFVATLQQLQSQQQQTQSQQIPIVPIVNQQVQQVQQIQQQQQQQQQQQQQQQQQQQQQQQQQSTQIQEEPIQQQQTSEKDQSERKKKKKKEKKEKKEKKSKEEGSVRQDLSQVEPEKSKNVKQEHEPEPGEIQPNYDFNDQEKSKSKGKKHHRSNHKSKKHHKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N525</accession>
    <name>A0A8S1N525_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0730013</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8084836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="VANTYFR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="54"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="122"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="156"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="190"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="224"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="258"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="292"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="326"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="360"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="428"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="462"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="496"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="564"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="598"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="632"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="736"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="804"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="838"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="872"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="906"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="940"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1343"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="604"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="997"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1096"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1344"/>
            <end position="1353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8084836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1373" mass="160700" checksum="BCBF9378D3DD2305" modified="2022-10-12" version="1">MNQDSNPQNQESQEECLQKAKDHFEKSRTLQQNQKIDEALNEVNETLKLNPNYSQALNLKGELLIKKGDLKQAQDELNKAIQLNPQLIEAYYNRSLIHRQNKIYNLAINDAEMCFQLDPQFVKSINLKGLIMKEQGSLQEALVVFQKTLELDKMNYPAHYQRGLILRTLGKHEEALLELNQAIETRPSSSEAFLERGELFQDMKRKSDALEDFNKSIEIDPKRAYSYQCRGILLKQMEKFQEALLDYNQAILLNPKVANNYVNRGILFKDTNEKEKALEEFNLAIEINPKFVKAYKNRAILYKEINQNEKALNDYNKILELNPKDVNVYIYRGNLLKYLGQNEQALQDYTKAIEINPNETENYYKRANMYKELGQNDLAMKDYDKIIEIAPKNSNGYWKKAIFLEELKQNELAITVLNQYIELNPLDENAYMKRSDLYKLIDKLDQALNDQSKIIEINPTNIIALKNRALLFKQMNEFEKALQDLNRILEIHPNHINTINIRGNLYKDLNQNDLAFQDFNKIIEIDPNIYIAYYNRAKIYQRYQKNDLALQDLNKSIELNLEVTFTLNERGNHFINMNQKDLALNDYNKALEINPKCIDAYINKGNLLKSLDQYEQALENFNKALEINQNNFVAYHNRALLWTKMNEKEKALADYDKAILSNPKSETTYSSRGQLLQQQMNDKDRALEDYNKSLQINPKQGDILIIRGNLLKEKKLISQAIQDYSDAILINPNKTDYYLSRGNILQDPVKDYEKALQDYNKAIEIDPNYFNGLYQRALCYRCIDKNDLAIDDCNKCIEINPKSELPYILKGLILKEMNKPELAIIEYNTALQINPNNQNTIIKRGDVYNLMKNQQLALNDFTKAIEISPNESFAYSSRANLLLSMDQKEAALQDYNKAIQLNPKNATLYYNRAIFYNGNQKKELSLQDYNKTLEIDPNYSLAYNNRGLLLQSIGKKDEALQDFLNAIRICPDNPLFLVNTGDRYYSDQQFELATQYYKQAQYQIENLTDQRKNELRLNQGNLNFIQTKVKLITKIENEIQKMKKQIAELPKTNTNTQQIQEYLEKVTCIERSVSYSVQPINQDSQSDAQQVMISYFQQMEKQLKELQNKVSAQDKVINQLVQQDSFKIEQEMNQIKQPQNKHQLTYFRSLFWHLYYYLHAMSEISTNLFQVNTNAMIESTSEKVLNIVKKAFNVGTKVLEKVHIAEHAFHIINEALNIVVDHKREEKFKKRLMLLTNILKLFAITPSELEREVQFTAIELGRTQQPGLKEIQNSKFIEFIKKLSEEESISEESSKDIYWKKGIEDTLIILKYLEKFNQKIIKEDQNKKLREVFLDAIKQNISEKSNKKQKDQTKTQSKQNKPKKENQEQCNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5F9</accession>
    <name>A0A8S1N5F9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0790048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5F9"/>
    <dbReference type="OMA" id="CEFLYTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="110"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="234"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="348"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8087660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="518" mass="60829" checksum="5C1EEF74D8C794CE" modified="2022-10-12" version="1">MTKMISSFKQKLNVLIKQQQQIIIIINNYVNQISLIGAILTLGDSQKCTWKNCIVEVVMSLTQMRSKQRSQLEVLQVSALMQRFQYCRDMITKEGFEKYNKFCEFLYTIYCRTGERVIRKGQQGVRFYMILQGQVSLVSYKPKNRKAQMMMDQEKETVPNYEDDTLFSAFSFKELNPGDHFGDMSLLDNGQVSCTIICKTFCQFVCMDKQQFVKFIGKSVPVLQKLKILEEQTIFNCWTDGELRALSYEFKNRTYHQEEFLYQEGQRNVQVFLIMDGQIEMTSTRDNQTYHLSSLDVGALFGDDADYHTCNAKCVSARAEILVIGRQVLSRMLDWHPESKQLYRQQLQQKANWRLERLNNLIESHQEKTKVKRTQSIKMQLLDLLIKPKYQKIRLSPCVKRVTNIQDTMDFEEKKLKHKEILEKQNLVISQRKIEFFKLGLTFTEKRRKKQKQQQAELKLKKQCESVIISEPPLEKLNRASSTIMTKTSQTTFKLSPAKKLRKVFSIEGTTINIHNRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6G2</accession>
    <name>A0A8S1N6G2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exportin-2 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0810041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6G2"/>
    <dbReference type="OMA" id="AENEFLM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF8">
        <property type="entry name" value="EXPORTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exportin-2 central" evidence="1">
        <location>
            <begin position="154"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="954" mass="110817" checksum="063C3858BE1247E4" modified="2022-10-12" version="1">MIVTSNIVNDLNTAFQLINMGTNDKIRQGEQLLQQIRGDIQYPKVLFDYFQAYEISLGLRAAIELKLWFKEYRNFDDYQAQYVQNVVQTIKQHIISAYIVSEAPLIHQLKDAIVYVASRDFPTQWPTLMADLNQFLTHPDYVYKTLKLIYKLTEKYVYQSRSDPLYEEIIITCDTIHHNLLLLAKSLIQQIEALQNLKLSYEILKTLLKVFYNLNFQDLHPHFEDNLQSWMEFMKVVLRLQPVQGVEQFLFKCKGEALRCVLLYAMKYRDDFGDLIQVFSSEIWNVCTQTSAGRDSDKIVLCALRYFKTLIAWQDMKTFFEQNIKILIESLIIRNLSLSKDEIGMFQDEPQEFIEKFFEQSDLESRRAQAVELFKTVTKHFNQQVNIIIQEYLQAYIQSGMNGIDNEIILINLIIEASTSSFTSKDGTIDIILSQENVLGFYTHCLKPKLGQIFELQQQNQSVEQKFTPIQLAFYCRYLFYFRNVIDKVELPTLATLVSKLQLSKKITLSNIASYTAYSLINVRQDVKNYANHQLYFENVNISQYLQLILTDCYNNIKQQQKLETYSLKLTNSLITLLKQEIFNAIAALCNLLQDLLKNIKLEYEFQNVHLVFEIIASVVDVCIIAKNSEAANQLQQSILNQLDELLRENKGDVTNFVLQIYSLFLQVQTNASSYYQNLLQNFLEIQNWNESNSSLFQAYIIFFQFGFQGTNLPTTQLQAILKSIVSHSTPSYTELSKFLIKSNQQWTNIVLAIIFEQYKIQQQSQPQISSKSKATHLSQRLINKEVFLLLAHVFTSYGVEVLVNECSKISPQLLESFLINELGIIKSISQRNERKLIFTMLSAILFSGSFLSQQSWNLVFQAMVENITLKKRNPLKFMGRVTDREVTSSSGFQPLKPVIARNNNDKLLNDEDKLFSASFKTYYSNPIVQQQLPIQQLLTQQQQELLSNLIQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9C9</accession>
    <name>A0A8S1N9C9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0690189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9C9"/>
    <dbReference type="OMA" id="LYMYLEG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="295"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="367" mass="43983" checksum="87093D81E0B21895" modified="2022-10-12" version="1">MDEVLLESLTHKFSELFKNISSLQEKRETVQDENEHLKQLIQEEQDQLEMNSGESSDDEITIQEIEQRWKQKLEEKKESDMQMKEENNLKSQLYMYLEGIQNEIIQMRNSIEQSKESEADLQQRIEETTQKIDEYQNKIQRAEQIKLQVEKQLQEQNEKYLQDSEKVKAVQAEVRNQLQANAMGDKRQNELEKIIQKQWEELQKRSQRIQVLQNNMNIISTKIQKSQKSENIYQKLKDENTILAQENIQIREKLNQLISALPQHMKKGNVKGKQKSKKEFLELYDESICSNQIKLRHLEQIREEHQSNINSLHSEILQLEEKYNQMTQNVKNLQEQYLEIESSCREFNEEEYVKKNCPNYYKVKKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAN7</accession>
    <name>A0A8S1NAN7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0700194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NAN7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="297"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="384"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="610" mass="72371" checksum="555C452A905BD9F9" modified="2022-10-12" version="1">MDSQNKKIEDRLVCVDCIAENPLIKYQTIQNVDNQWKEQNSLSENCLIELKMESKIKKSELLNQIAQMRKNYNKKLNEISDKLISEQYLCNDQIKESNRIKRTSIKVLDNEQLIKDLKQLIEQEKENEKVPQNQIIKDIKNKDSIFKKDLEYHLESLKQHYQQDIQQSLDILKEISKESNLLTKLSEMIQQIEKSHQKDDNYQNQINIIKEIQELVDDAKMYQCQTNLFEQTKKFYQSIKYKIELIQQNIKLKSKENQTESKSQLLQSQYSNIQNILNEYQTIFENNSIQMKKYCNIKQMEDDLIKLTENNNKLEIESNILQQQIENNFINSIQKQLEQKLNEINSKLEIKDKEQKDIKVQYDLAIKDNINMKNQYEQEKLTMIKKFQDEQNKLKQELNQNINQKNLELQEAQKKLDQINKDKLEQDKFNKMQLEKIQLYQKSLSFSNAYKNGNCQVTEGGKVVADVGNSSWSYCLCEQAIPKTGKIQFAFQILNGSAFMVGIGFREIMQKHNYYQCYQPGYGTYLIENNCTIFSYHNKDLFLKKFSFQFTNNEIIKIEVCIENKYIKWSRYNNPQLMFVQLDIDTSQELYPCVGVSGQSKIQLLENIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NB12</accession>
    <name>A0A8S1NB12_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0850141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NB12"/>
    <dbReference type="OMA" id="EIFPADH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8090187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="385" mass="45354" checksum="A28046B478A83F4C" modified="2022-10-12" version="1">MFIRNEMKSALQSGDSFLLSNKSVSQEKMSQEQINQQSPKKRSLLKQKHNLIPLTDRLEIRKCYYNEQQNEEQKSQEHRLHNSLQKNKINLLPKLEGLESDKSLYQTVIEIFPADHPIWNDLVQKSQTIEEVIESNPAFFLGLMTLYVQQSKLQTLKNKFQQKAIQSQYQQDYQIVNLRNQDVLLDQSKAHDQMWGKNLQQLYNGSVYNSQYLPFKLEKKRKSPKKDFNPNLSQSSYLSTYRSQFQNWKPQYPGKMTRETSSVEPSNLPFISESSYAREFKNIKTERSPLQKIAKLGPFAENSEQLVKESTSSYYQQQQFNRIPSKLMRPISPKLFNASFEGQYTSEFNKSYHINSDQESSFANRFYESNSVKKIFDRKFDSKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ND99</accession>
    <name>A0A8S1ND99_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0780222</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ND99"/>
    <dbReference type="OMA" id="FKWKFSS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000064">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000015">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028934">
        <property type="entry name" value="Vps26-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12233">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 26 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03643">
        <property type="entry name" value="Vps26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8087483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="35059" checksum="B61D251CE0019BE1" modified="2022-10-12" version="1">MAVLLNLVQGAPIIDIYLDGLDTRKTARFNDKQQGLIRLPVYSDDDDISGVVDLRMNKNKKIDHLGIRIELIGRIEILNDQKQSSDFMSMGRELDAQGILMEDKTYKFTFNKFEKQYESYYGKEVKLRYYLRVTMNRNYGQVKKEIEFAVQIVDKEQENQPQTSLKLEVGIEDCLHLDFEYFKSRYHLRDVVTGKVNFYLVKINIKYMELAVIRKEQIGQGNNQQTDNETLVKYELMDGCPQKGEVVPIRLFLSGINMSPTLKNVSGKFSVKYILNLILVDDEDRRYFKQQEITIYRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEZ3</accession>
    <name>A0A8S1NEZ3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0840154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEZ3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="62" mass="7660" checksum="FAC68608F05DD8F6" modified="2022-10-12" version="1">MTFINKKSKSNLFKLSKDRNDFLFYKFLNEENFSQTFLQQYFKRISYEGILVLGWQFNLVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NG22</accession>
    <name>A0A8S1NG22_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0850172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NG22"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="144" mass="15915" checksum="3F3C52E8C5C24379" modified="2022-10-12" version="1">MSQRPPAPSNQPPQQPQAPQYQPQQPQYAPQFAPQYAPQYAPQYAPAPLATYPTQYAPAYAPVAPLTYSVARPVAPVVAQPVVAQPVVQAPVLQQSVIAQPVVQQSVHATIKGESRIEYVPYQKAVMEYEEQEVRKKSHRLLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGJ9</accession>
    <name>A0A8S1NGJ9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0830192</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGJ9"/>
    <dbReference type="OMA" id="PRIQPTH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8089436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="667" mass="79414" checksum="499906AF33C69E47" modified="2022-10-12" version="1">MHNLTTSPRCPFKFPTEHLKQDSKSMTFRDFIQMQQTKEEMKFQFPGRLTQQQGSPINSKKQRWEKQLSTLLRQDPQNELFIKHSSQCQIAMKDFINKTKNFRSHEQSPTKTTYHLPMISDSQVIVLPNTPVHFKFTPIQTKTKASNYVGLRNVQDQLEQLKKPRIQPTHRELKQQPMTQTEIISKFFETKFQKIIKTQIKQYGSNELLDQHSETLYSRIIQDRQLKMKFQGKSLKYLKDIYKSILGIGYTQEILLDPFRMRSIHAPLFIKKEQFIRFKYLFINQFMDMETPVELLFKGCYKLENFKPLILNQKSDFEIFGGEFGINEIAKNMYEKIFKDYTLSPYFKAIELEEQAIKFAKLFAQLIDHTESPNYTLEVLRERHVKYKLTHVQLANFKFYLSTTLQKLGIRYKHIRILLRKMDTYKFAILNKSSLQECINNSPGGYREFIENFVRLCSSEPILFDLVQKRGKQRFTAHCENVFHYFFRDNVKSITDSDIETIHKNKTIISEKVFKKFKEKAMQAVSKVTNDPILLSDFEEDWEEITPILLNKPRKTAIKQLGGQFVINRIASKLENEIMQRPLLYKVFEDNESPVGQNLRCKLNLILYGLHFYKRTDIEVLHKRLRIREQPYFEFQQAMKTVMQDEPQKLQFILDVIDDYKKHIVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NID1</accession>
    <name>A0A8S1NID1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0760216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NID1"/>
    <dbReference type="OMA" id="KDINYRR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="260" mass="30642" checksum="B0F73C3E125CDC27" modified="2022-10-12" version="1">MGNGCSGQREMTERSETLRQPSITLTKLIEQDKLISIVPTSQLTQIEIPNALLPSPSRQSISFESMMTSKVRVPSEESQFQNQFTQIKEVRLTNNQSHRPKIQVPQRKHKKQEIYEIEHQYHSSSPKKQKNRKQISPLENQQQQQQQQQYYDYKKTNKSNRKKSTSNQQKMKQFNEIEIISRRKYSDLPSRTEKTQVKRKISNSIDDHNFIIGTDCISKVKSYTPSPILKRKESDCETNSFQKKMVRFKDINYRRPGFVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NK78</accession>
    <name>A0A8S1NK78_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0840171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NK78"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="159" mass="19115" checksum="DFAF65F2ECD48E47" modified="2022-10-12" version="1">MIILISIKNQYFSNCKVLDIGQYDHGIKQGRWDIKTIKYYEHDVLTYYFWPKDTFYIIAGGNYQNGEKNGKWIDLDENYNYHNQILYEGEFYKDLKQGKWDMMKHHNSFINRIGGGQYNQDGLKHLKWIELDKNRQKKLILVEYQNGIKICIESMQTVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NLK6</accession>
    <name>A0A8S1NLK6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0920091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="354" mass="41939" checksum="51C90D941312386A" modified="2022-10-12" version="1">MKSLFAIIKTRDEDRKILYASEEQFNLDQVFNIRDGENSDIVTYRDKITQFFQQFQYFGLIQKSNFLGYSTNFIIDFFSQERKAQCHELMIEDLIFYIQTIKEPVSITLVLVIQKESQYNSQYATYCLTFIKKCLQKIEKESKALTRMSNLIIQNISQFEKEINAQIPEYEKQKCKSIQVRDNVTNIARKYLDSCELRRNLNQFCQAFVNRKMINFFQIQMTPPQQDLMLNPIAIVIKPYQTLLVANFIGKDDNTYISQRFLTFIQKMQPTKSFQEMSNLYYFSXLRDLRSIINEILIMSVLFINLLFTFIRSPINSKLQSLKLRINCSSQAVTILQYYLKSVMQPCQIFECLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NM27</accession>
    <name>A0A8S1NM27_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0920183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NM27"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8093222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="33" mass="4079" checksum="F3B18D192103DE3B" modified="2022-10-12" version="1">MLSQLKHQKRESFNIQNVVQMIRPRFIYFKGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NP33</accession>
    <name>A0A8S1NP33_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0800228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NP33"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="83" mass="9560" checksum="888A907D120DA08A" modified="2022-10-12" version="1">MLQHARIFKGCKVREIVIIKSGNLNKITSTLDCAISFTVVPCTQNEHKKMLVILVCTMQLQKFTYCNIKVDTKFDNCTYIRCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NW35</accession>
    <name>A0A8S1NW35_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0950038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="DLMECEA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1330"/>
            <end position="1354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1370" mass="160352" checksum="E602A1968CD4C6F9" modified="2022-10-12" version="1">MILSLLILQQYFRYTNTKCTTLNDEHHFELSIGERFDLNMNDNFIGGNLRFSLVGSHSRQFTVQQRLHLVGIQPLIEDYVTIKQWGELLALLQGTQQQYYIQYGMCDFQKAPVFENIIQLLPRKNCVDLTQDSLSSFIVLCVEETYYYLQYVQIGGQIQEFQYQMNQKSKNFIINIINNIVIVIQIAEECIIDTYYQKKAGMQLNAQTLSELMMQQISNLSINDITIKANQIIIAHKLNFIVYLTVEIENDQPIFILIKSLVFEQNFLKISAYLDNFLNLIVVALIPENRLYFIETNKQYQLDYNISIKQANLQVSSTYCSIHDGKNLIIYKFSDYLKTLMFYEEIDLIQYIFNPDLNDLIIVQEKQISRFILSDGYLILIPPKEIIKTPVEFSVVASSDLMECEARVSYIVTSSVNSQLLKPYSYPNPYPNIIFFPKFPSIYSLNQISISGPNQKMLVTVKENDWQLINIITDYKKIIPIQGLNKKQLKLTSINIEINKLINSKIHFLLQYYQHQQLQTEFLSCEFNKQQLKIDCDKEYIIDIDNVINQDQIFYNYNMEKNEVFIYVVNVNEIIIYKWFINQLNQINKIMLFNENNQEMQLTISQILQINNLLVILRGQCVYLYQLLGDAQFRIDQQFLQKKNYLNEFKPSQVKVSQIWNLIFIVNGYDILVIQCYETCDLVNKIVIENAMSLIIQIQLDKVLIVLAEIESELSLMEYLIKDPHYIVFQKKFESYHFWIQKQMLNNYQDDVFYVYAYSPVLKQRVILIYKVLVELRDSLLTMINISEDNQQSLCSVSGMNEKLMYIHMEKQDIIYLLTNDLRSTINVDNKFDNLFIHTLQVESSFFNIDYPLQQKYQQTIKFVDLKTIIYKKNNLIELKITEDTKIPIDTLFFTGLITNLKAENALLQIQKSVELTDTKRIFKSDVINSCQIDQITVILTLNSIQFVYFNLIDYYESLDITDLKAELGNVFCSSQISKLIAFRYFNISGQYIQFIQCTQQKCLKQGNPQLMKEDEYIFTLMIINSTLITLSKDTKYNQTLLNIYDIKKNEFQISLYLVDKIDKQKANQDFNIKYISMQSYQVNGIMIYKLFLTENNNGLLYVDLAQEKYMTIKSNVINLNLQTLLQSNGLFEVDDQLYLSTLSTQDPKDFHIEGYTEFYLLIITTNSLHYGLIIQFRGSKLSDTTIAYLLHTHQLWSVVSFTQVKLSYLALGYLVPDKEELMIAIYKIPTTISTNLKLSPILVHGAITTSLDRNLNLLMYLLENDGQDVFLYFKDKDSFTIFQAKIFNDSYLIINSSVKNHEFTLVASNYYYENSIQILIYNQVKKGGFGVVMVLLILIVCILVLALLGYLFFKIRSKDQVVDSITLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYJ0</accession>
    <name>A0A8S1NYJ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0970026</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYJ0"/>
    <dbReference type="OMA" id="YNDWLGS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="586"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="331"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="611"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8094775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="677" mass="80957" checksum="DE9A49E8C7110487" modified="2022-10-12" version="1">MSKLYKNASQSNFYQSQTVPKFRSPNPVGPSLPKYNDWLGSEEGNTLLNALKKENHALKEHNKTIEGYLKNVVKVFESLNFENSNLRKEIEQRTEDLQRVIESTATNQTLNEQDLRHQIQLYEDELGVLNQHISNIKGGDQLQKSILEEKENQLKKSKELIQKLESNLNSMYQKEKDVSNEAQTKDIKIKELTDKYNELNKKQKPLEEKVRQLNQQNDNLIQKVNQLESAKKKLEEDEDTKFKDLYTQNYSQKTDLEHLRHLCNQQELLIKELRSELGSLKQENYPQQIIILKQELNSNQQELILEQKRHSNAQQREQSQNEKLTSIKLQLDGLQTKYNLLLSDYENLKLEYDNDLEKLNQKQKQMIEKQKLANQQLISDHNLEISRLEFRIKELTLQIELLQKENQQKDKQLLEMKQLLLDLENLRRELIEQKTIIVQRDHKIEILTQQYNEYLESSKKKYQDLDDRYNNLQELFRKEQEKNDTLFKQNEKMQWYKEQFDEMEEKIRNLKLVQENYKIEINTYQSKISRLEIENEEVKQLHNTNEQEIVQIQAYARKLEKQIQEIELLKLENKTLRETNFREAIAFQTTTQTHNRRSTTSKQDEQDSGSKEPTNIQTRGFNVTSNKTLTTQPSHQQLKQQLQKTSNQNSNHLLQTYDVQNDLEYRNYPYRTYYKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZ77</accession>
    <name>A0A8S1NZ77_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0910136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZ77"/>
    <dbReference type="OMA" id="CHNQLID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="187"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="277"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="531" mass="62694" checksum="10A677A60050DC3B" modified="2022-10-12" version="1">MQPFQHSRDDYLNKQVYKLLTKASNMHMKTCEVKVPTRRNSMIESYILTNDIQQQSDGIRKQKIDKNRSCSNLQDYQEESNSTFKQLQFSQLPIMNKNTKLIMLRVENKLKNFRFAKENDHTVSTSTIAKMQSFCLDNQCFDFYSCTDLLLLKYLIYLLLQEINSLNQNYSDQQLLISQKCVNKDTIETLQDNVDDLKNKKHRNSIENLDKQINKMQRLIGEQLSLPNYDMFTTPQSNIKKSEFVFQPSSNTQKIKCDQSQSSFNNQKMIQFQIDELKMKNQTITKLTSGINNLEEELKFVNNQISDTNLNLQISQKSLTVKDQELKDLIKKNEKLVETTNQTIQEKNRLQDQYKSVKNDYELLQTKYNELQKTNQSILKESQIQKKSLNHTVESSQQQMKKEEQKLQKELLDKNKLIRLLSEDGKILGQYLKDISYKIQQIPTDQIPQNLQMLQRELYLNECLINTKLNNMHLAEIDLVKINNVNNEDKKSRKFMNCHNQLIDQMKSFQIHSDLMAMMVIQSEIIEKFLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P2G9</accession>
    <name>A0A8S1P2G9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Insertion element IS150 protein InsJ-like helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1000050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P2G9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055247">
        <property type="entry name" value="InsJ-like_HTH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13518">
        <property type="entry name" value="HTH_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Insertion element IS150 protein InsJ-like helix-turn-helix" evidence="2">
        <location>
            <begin position="40"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8096014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="192" mass="22048" checksum="48A860415E5A037B" modified="2022-10-12" version="1">MIHSEEESVNSFTPESQDQNPEEHKNKDSKKYAIIDQSIRIQLLRRILSKQATIKDAAKEFGVNFSTAKAILQTYRKEGRIGKKKTRERNKNKQESGENSSPRKIQSMYNLEQPQQMKAISPEQKPQAPQIQPIFPVMSSPNLDNTGAQIALALCQRELAQQKLINFQLLMMIQNFKNISQLQVKEEPNIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P3W3</accession>
    <name>A0A8S1P3W3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Synaptobrevin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1040100</name>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1040101</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P3W3"/>
    <dbReference type="OMA" id="SAQICRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF168">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="5">
        <location>
            <begin position="6"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="6">
        <location>
            <begin position="118"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8097554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8097556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23888" checksum="9F50919639A84753" modified="2022-10-12" version="1">MILYTLISKGNLVLSEYTELEGDFTEMARIVLKSVKPNQQKQIFSKNEYVFCITCISNLRFLCMIHNSQNKEKAFAFLEAIHQEYSNCSKNSDFNNGQFSAQLCRMMKGFNQLDQKDALTNLEETVEDLNESAFQNLRKMIEREVRLEVLVKKTEELTKNADIIFKYSRNVHRATRGSEFRFGGKVLILSLVIFAILSLYIYYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P4H3</accession>
    <name>A0A8S1P4H3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1050029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P4H3"/>
    <dbReference type="OMA" id="DQVYVFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="277" mass="33129" checksum="86A2C03BCCCC5732" modified="2022-10-12" version="1">MKFSFLKHNLAELIGKAEQLDSPSSIIKKFGPFLITSLFNQKLQESQEVINYMEKYIDKSNFIRNITQDHNAKNWRTNYRKSVESLACSIIVDNQNIIDKHYTNTLKEDQITLPEALPLVYTLVIQKIKKWNIMYEQEVLQRLNYLLNTQKQEQQKMNLNILYQKYFLSDLGLYYPELLVYSQGKIYCTELPLDYFIGELKVEYKDFFIEDNNLVDLQIGDQVYVFMYPDEFEYQTIKSFMLKYVRIKRLQELYSNVHVVWCYKGMKKKELLDAISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P6F5</accession>
    <name>A0A8S1P6F5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0560033</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1070155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P6F5"/>
    <dbReference type="OMA" id="NIVTCAV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21455">
        <property type="entry name" value="DLC-like_DYNLT1_DYNLT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.40">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005334">
        <property type="entry name" value="Tctex-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038586">
        <property type="entry name" value="Tctex-1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255:SF4">
        <property type="entry name" value="DYNEIN LIGHT CHAIN TCTEX-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255">
        <property type="entry name" value="T-COMPLEX-ASSOCIATED-TESTIS-EXPRESSED 1/ DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03645">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13399" checksum="DD99F25A7742F144" modified="2022-10-12" version="1">MADDGGADKGEYIGEEVAKAIDDGINAVFQLKEGENPPATLIYSKEKVISWSNQLLDYTNRNLSKLDKPFKYCVSCIIQQNNDSKFTVALGASFDTNTDGICSAQREINDIVIIISVFAMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8N6</accession>
    <name>A0A8S1P8N6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1080172</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8099108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8N6"/>
    <dbReference type="OMA" id="SVQFRII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="2"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8099108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="119" mass="14202" checksum="AF53BB0366F27AFD" modified="2022-10-12" version="1">MNYYNILGIQNNSSQEQIKIAFRNLAKMHHPDKNPQSVQFRIISEAYRILSNPQLRARYDQAQQQYEKQLQIYKLIRNFINGNGEIPLIHQKDFESNQNESKTVDIQQIQKKKIRKTQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P903</accession>
    <name>A0A8S1P903_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1100060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P903"/>
    <dbReference type="OMA" id="NTSQDQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8099626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="86" mass="10110" checksum="FFD402B1B0D6F8C5" modified="2022-10-12" version="1">MQSFYKLAFQTLSSFHSLGLVSDEEKSYLKEVIINWSTPQLNTSQDQMSVLVLQIIRVLRNQVQHVYQTQKFLCLIEEETDEDENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBY6</accession>
    <name>A0A8S1PBY6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1120063</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PBY6"/>
    <dbReference type="OMA" id="NYQHENA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF3">
        <property type="entry name" value="RETINOID-INDUCIBLE SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035869525" description="AB hydrolase-1 domain-containing protein" evidence="6">
        <location>
            <begin position="18"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8100351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="46213" checksum="A5C6DC3E1DC3F471" modified="2022-10-12" version="1">MFCLILIFQCYARLILQELESLKTQPEESTLPRQHCTNHNAQTKYGYLHVPNGAISFIYYQGRGQHNIVYINGGPGLSSQISNFLEIGPHFGQWNNFSNLLFLDLPAGTGYGRTNTTSNLSYEDVAIDYEIAMQNFNKLCKLTYDNVILFSTDFGARFALAIANRSKTIKCVGLLDPFLDTLNIVTEIPNFAFHMGVIDYQELIYFEKAIIKLSDDIYNGNYQHENAKFLKQLYYMTGNVSLYNVLEQKLYSEDEDKLEQILNDPESLYYIPFETEFLARSQNVFDQFKYNFFEPFDNKIIQHLLSNKNILVYQSQYDMLIPPSGTMKWLISIQYELDDFFYQNQLIKLMKDDKLIGLQKRGGYLEYLFVLNTGQVMHRDNCNNSMILIQQYLESII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCI3</accession>
    <name>A0A8S1PCI3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1140065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8101012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCI3"/>
    <dbReference type="OMA" id="TPKIHLD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="262" mass="31635" checksum="34AF5DD7B18E6CF0" modified="2022-10-12" version="1">MFRNLRQQYQKDLDYHFLKQFNKSSMNLRYNNQKSPFAIKKQLSKVNIALQSQLNKLIEYNEKISKFQKIKYYVRSRSSQNSQEGHLNKRISHILLSLPFHSIKMHQKVGIRQLWRKYIFIIIFLLRYQKQRQEKLKLNLQNRHIQLGTPKIHLDATKKRRRNADSQFKLSTFAEYIDAQDKQESKTKRSEDEKFKKSPVYQSFKYNDVRICRLLRFRNNSVLTSNAESPLKINQQVIKNNKLPPLLQIDRRMQRVYSNLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PEH4</accession>
    <name>A0A8S1PEH4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1150016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PEH4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="59" mass="6993" checksum="52C714F340719689" modified="2022-10-12" version="1">MSNRSKQNASKKDQIGEHYHQIIFISVITKQDNKINISKYMRICQKDVENIRQIICNVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PG48</accession>
    <name>A0A8S1PG48_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1170004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8101879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PG48"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="130" mass="15653" checksum="938677D13D1A4A19" modified="2022-10-12" version="1">MFNSGKYGNFICQYLILFDIISIHLKHLQKKSLPPIKSSFSLRQNLIIPINFENNDKKYAFKMMNILKILILIKKKVAILESLREQLWRWRRNLKYDRSLFIQINFMEIVQKNLNHTSEALLFLKQKSDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PK31</accession>
    <name>A0A8S1PK31_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1200145</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PK31"/>
    <dbReference type="OMA" id="SWHETNL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052187">
        <property type="entry name" value="MFSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8103299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="443" mass="50862" checksum="A8B2DC37B663C7F2" modified="2022-10-12" version="1">MVKALSWHETNLRWFVLCLIGVYPLAQFLIAEFPALLGEQIKNYFQVTQEDVNYLLLFESLPNMIMTLVGGLIIDAFGVRRSYVLFGIVVILGQFLCLISVFLQSFKLMIIGRFVFGIFESSGCVAESYYINKWFKDKENSFAYGIDTALCRIGSITASILYPFLYSASDNDLSRCLLMCFKIAIFSFITILILTQIDRFSDMRDKVEDQKLESIDLRQIKNFSLEFYITLISCATCYSVFFIFSYNSVEMFKQNFKLDQNTANILFSIPYYLSALLSPIVGYYLQRIGRNIEILIIACSCQLLTLTMFQMLPEFDSPCLIFPLIGSILNGLFFGIYFAVMWPYIPNIVPSHMVATGFGLIYSCINLELTCFSYIVANILRVENYENYLKMNSLLLLLSFIGFISLIYLLFSNQSKQRNSNHSQSNETDSTIQIELMVLCKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKK5</accession>
    <name>A0A8S1PKK5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1220037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKK5"/>
    <dbReference type="OMA" id="FRTDPTY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047134">
        <property type="term" value="F:protein-disulfide reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045108">
        <property type="entry name" value="TXNDC17-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010357">
        <property type="entry name" value="TXNDC17_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12452">
        <property type="entry name" value="42-9-9 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12452:SF0">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06110">
        <property type="entry name" value="TXD17-like_Trx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="14"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8103795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="125" mass="14393" checksum="BB2AB30FFC1F042F" modified="2022-10-12" version="1">MEQVIQVTTSNYSEKVNKEGKAALIFVGDVLAETGQSWCSDTVEAEPTIKNYAIPQLLQKGYTVYWCWAGDKETWKDPNNPARVSEWIQIKSIPTLLLVHENKEAIRFEEEQLFNENALKQFLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLN0</accession>
    <name>A0A8S1PLN0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1220040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLN0"/>
    <dbReference type="OMA" id="GLTQGKC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8103801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="346" mass="40769" checksum="09F075D779FAC106" modified="2022-10-12" version="1">MDQIVYIPEIRYKQINEITIDSQETIQLKYQELEELIKDIQIHDEVEFEHIFTLIRPNCCGCGPYSQKSEFNVQGLGQLEFYIDKYHCCTLWCFQCFWQNCYWCFCCCFYECLIKLIWCILRPFVKCLIAIVKCILCVIQIHRLPNQLYVMENSNIKLEFWQRGLGTIKIKNQIILNFVQTDPNFYDKYCSGLYSSYPQLVITSPFDQNYKLILKPNKNDCPQSIKGSMSLMAFCDMFTTSKYYYVDGLTQGKCEIINFRTALQAIAKISEMQGQCCKFCPLIDYPRFAIQFQNVRLIDKIAIILGLIHISMFNQWGEGNASYLRLKFSKEICENRNRVKSVTIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMD8</accession>
    <name>A0A8S1PMD8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1230012</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Ole e I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YWNTQQL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006041">
        <property type="entry name" value="Pollen_Ole_e1_allergen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31614:SF5">
        <property type="entry name" value="ALLERGEN-LIKE PROTEIN BRSN20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31614">
        <property type="entry name" value="PROTEIN DOWNSTREAM OF FLC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035780743" evidence="4">
        <location>
            <begin position="18"/>
            <end position="2390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="712"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="716"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8104121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2390" mass="267816" checksum="66C7119396118656" modified="2022-10-12" version="1">MLKSLFLLGVLFCSCLTKHIEYQIVELEEVNYKIVIKVIDFSAYALSGVIINVKNGNSDIGEKNLMTDSSGTVTITGKFAKQNSYQIIAVASTTFNNIQASAQINGIVDQSGTIFFNGKVQFTQSDVYVKDEFQRGLIFIKTLSNSKPLPGVLIKFTAVYLDVSKQTKEVNFLAITNEKGEENVRFLLPTTGGSFTILYTASKYEYWNTQQLDRNSQHYILWPEQKYELSVNIELQPGQFQYLLTALIQDSNLKPLQGAKFQVTGTNLGSNQQLIENNIISGSDGKISIIYNLKATPKLHLKELVTKDGYQSYSSEFDQDFDLNIKKDQFTKDLGIIKLQDASGDLQISGIIKDSSKIFQFVQGAEIVFTIIDSIQPVKVLTDAQGKFNVHIFVPSNQNIYVKAIVKAKGFIDQEITPKLINVPSKQPFLLQYDIVLDRKVNEIIIKDQLVDLNGNPIQNPKIKLIESNPPMNDKSLYNKEIIGKQDGTFEYKFACYEDQKFTATFVFQVQQFVPVTYKTAELECKNQDLIKTSISLSKSTSLLTIKGKVLDVDQKPMKGLQLTFKSDPNLTIANPNLVTDANGDWQIDNLTVIPQNNYKFTIEYMNDIKQLTQVSSTYTPSMDKQQTYTFPIIGYQRFVKVKLTGRITPSDGKASVPIPLVITCDSKGRDGNPIQVETTTKEDGSYSVDLETLAGSDKPLQCVITNAQIKLPGQSGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTNPVSTNPVSSVQPIKINVEVKGPSWSSSTNIPVTYNSVDITIKGIVSDKTKTIGGIENTDVVLIITPQDIYVQNKHLLEQYLFYKRHYQNTLSQSKLSTKTGKDGSYIFSFKAVQAYTLKFQIQAQNPLFKTENKIIEEKCLQSITLNENIELDRITILTKLHSTLFSSDKKPIPNATIKMTQSEPLMKDSKYYNIEIKDDDKGSFYANFECYKDFEYIVTFQIDVPQYEEQKIKSSKFKCDKEQIELNPITLNLQKLEIKGILAGKVVDPHGKPVANLPITITTQPESATITTKSQSDGTWTATIPKIYPNTEYKAIIQYQDINKQYQKLEQKFSVQSDNQKISVQDINYIDFVDARIIGKIELEKGKLSQPVTMNLSCQGFKDSKGPINKDFTTDKNGNIDISFQVLTKHDDKIECVIKPKDIILXLDLLDQLDLQDQLDQQILSQLILFQVFNLQKLMLKQKVQVVTYNSVDITIKGIVSDKTKTIGGIENTDVVLIITPQDIYVQNKHLLEQYLFYKRHYQNTLSQSKLSTKTGKDGSYIFSFKAVQAYTLKFQIQAQNPLFKTENKIIEEKCLQSITLNENIELDRITILTKLHSTLFSSDKKPIPNATIKMTQSEPLMKDSKYYNIEIKDDDKGSFYANFECYKDFEYIVTFQIDVPQYEEQKIKSSKFKCDKEQIELNPITLNLQKLEIKGILAGKVVDPHGKPVANLPITITTQPESATITTKSQSDGTWTATIPKIYPNTEYKAIIQYQDINKQYQKLEQKFSVQSDNQKISVQDINYIDFVDARIIGKIELEKGKLSQPVTMNLSCQGFKDSKGPINKDFTTDKNGNIDISFQVLTKHDDKIECVIKPKDIILDTGSKVELIPPNFEVNGLTIKSKFLSTLFTINGKLIDETKIEEKLSGISINLNLKQQDPLTNQQINIKSLNDGTFTFIFELLKGINYDVTITVEAQPQFQITNNNIQLIGKEEKQSINLSISLKRVIIDCIINGSLIDSKNNPIQNAKVEILSSIPQMQNNLLYNKLVQANKGDFSIPFQCYNQLSTTLTMDMQSDAFPKQPLPPQTFTCGEKDLKLKPIQVSTKTVQITVSGVLTDKTGISKNAQNADIEIVLTPPDPLATNLKTKSDTNGKYTLTFGAVKDQTYTAVIQINHPEFNPFKSSNIAITTNKDQTIIQDAALDRIILQATISSTLTDPQGKKSPISTLTVESLQPIVKGTEKNKITSKTDTNGKFNLQIPCYKNTEGSFILDVQADKYKDTKSDKISFLCNGPQIQLPPIKIKQELTPTNTKLTVGGEIIGPKGKGQSGVQITLITDPVTKEYNAKTGSDGKWQLLDDQLQFGIKYQVTEQFLDASGQLHKITLPFQTNDEKSELMFPQQLYDDYLPFEVNGQVTSEGGIAPTNVPVSMQCDGFGQDKRPIDIKTNTDNLGLYQIKWNMLMNSNSKVQCFVITQETAKFKQTSYQFYINPNSEIVKVSTQAQVTPEFKGQLNKYYQYSSISGVVMAEFDCDGRLDWRGLEGVNIKLKQWNGITFNDLKTETKSDKNGLFVIKYQVLKSQIQNEMRLEFNKENYYPASADFNIYSFDPQPDGTYLVQLSNIKMVKIGVADKCPQKKRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PPX4</accession>
    <name>A0A8S1PPX4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1270087</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PPX4"/>
    <dbReference type="OMA" id="YGNLDSF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000016">
        <property type="entry name" value="PIK1p Phosphatidylinositol 4-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF22">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="5">
        <location>
            <begin position="332"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8105467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="612" mass="72632" checksum="61196B845DBCF2D8" modified="2022-10-12" version="1">MLVTTFNRRMNQLLYSLQDKQEHIIQQNILDLYNYILDPEFNQYLFQLCYLFYVTEDKMLKELLYNYALTNNANYYRLQWTLMALNENSNYENVKNGFSTFINVILLKFMLNQQLKEEYVLELGTQIGNNQKSINNIDQMMKNCIKSYREIQDKIIFNLIEKSLRLKKIDIENQKDHMSRFLSQINKMIANLRIQEQPEFRGLTIPFNYGNLDSFLIVNLLEDEFTCFNTAKRVPYKIIIETVNPSELLLIQSVNQLCQENQSELIPFYDIEKEMKTISQIKIYQQYNIEELKKLAFKKIDLQNLSHIQNLTKIKEKDIYVSKSQNKSIWGEDWNITKQKIKQNSQYGNLKSHDVRQIIIKGGDDLRNELLIMQMMKKINEIFKQKQLKLFLRPYDIILTSANSGILEFIPNTVSIDKIKRDNPNLSLRQFYLKNFENFSDAQKNFAESLAGYSLICYLFQLKDRHNGNILIDNEGHIIHIDFGFVLTLTPGNIGFESAPFKLINEYEELLNGKDSDIFTYYQVLIFSGLTILQEHVSELLTFVKLMNFSTRNNLLACLENFNMIEFKKRFHENVNQKNLYAIVENLVKKSSNSWKTILYDYFQYQTNTIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTM1</accession>
    <name>A0A8S1PTM1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1290001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8105938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTM1"/>
    <dbReference type="OMA" id="KECKXEL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="209"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="229"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="251"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="293"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="335"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="377"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="419"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="461"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="503"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="545"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="587"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="629"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="650"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="671"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="713"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="755"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8105938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="882" mass="97813" checksum="69372810326D433B" modified="2022-10-12" version="1">MNCAQEQKEDLYKLLASTKNIDEKVFRIIIEMLRKKNNSDCLEFLLNEDNQKYNGLYIDKEQKLNDVTNDIKKITTVIKNIGEHDFNKNNYTEKIYQKKKQYLINKISKEKTIINFFKFLVHLTAIDKKMIQCGSNSLHLLVEMKVDLKNQCFENIQIRNTSLIGANFVNCNFSGSQFENVNISGLNLNGAQLFYCKWTNLLINELHQLNGHSNSVQSVSFSHDSTKLASGGEDNSILLWDVKTGQQKFQLEVHSSSVRSVCFSPDATLLASGSDDMSICLWDVNTGQKIFKFDGHIDSVNSVCFSPNGITLVSGSNDCSICLWDIKTRKKKTKLLGHSDYVTSICFSPDGTKLASGSGDNFILLWNVKTGQSKFQLEGHSDSVNSVSFSPDGNKLVSASDDCSIRIWDVQTGEQLSKLYGHIDSVQSVCFSPDCTALASGSSDNSISLWDVKTKKQKFQLQVHSSSVRSVCFSPDATLLASGSEDNLINLWDVNTGQKISKLDGHSDSVNSVSYSPDGTALASCSDDYSICLWDVKTGQKIIELQGHSDYVYSVCFSPDGTALASGSADNSVLLWDVQTKKQKFQLQGHRDYVYSVCFCPDGTALASGSADSSVLLWDVKTRKQRFSLQGHSRNVTSIRFSPDGTTLASGSDDCSICFWNVKIGLQIYKLEGQNFEVRSLCYSPDGAILASGSSDNSISLWDIKTRKQKFQLQGHSRTITSISFSPDGKTLASGSNDRSICLWDVQTGKKSISLEGHSDYIQSVCFSPDGTTLASGSGDKSIRLWQIKTGQKIQPQNKDQQGIFEKLKIPFLNNSFSESVNSNFTILTISNQPPVFSAQGALIFKGEFINHFGLDLLLLFKQRGSCILESQSEFKQNTKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTT7</accession>
    <name>A0A8S1PTT7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1290035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8105998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTT7"/>
    <dbReference type="OMA" id="NWSREST"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000026">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="8"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8105998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="140" mass="15622" checksum="8C6A45279B0367E9" modified="2022-10-12" version="1">MQGAPEVPRRFRLLEELQNGEKGIGDGTVSYGLEDAEDMQMTNWNGTILGPYNTAFENRIYSLKITCGPQYPKVAPQVRFVTKINLPCVQPNGQIDINKFSILKNWVSKSTLESILTGLKNEMSSAANKKLQQPPDGQTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTZ0</accession>
    <name>A0A8S1PTZ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquinol-cytochrome C reductase hinge domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1290075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTZ0"/>
    <dbReference type="OMA" id="HNMIQNG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023184">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02320">
        <property type="entry name" value="UCR_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome C reductase hinge" evidence="1">
        <location>
            <begin position="19"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8106048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="91" mass="10675" checksum="7AEA1A687C46D08C" modified="2022-10-12" version="1">MNSENYKTEIHNMIQNGKDPKDMVIQMCRPQCKWYDDKYDRCVKAFLSLKNADPEKNCMYPYRDLVTCVEACVQPKIQHALRGNEHGSIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1I6</accession>
    <name>A0A8S1Q1I6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1400151</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1I6"/>
    <dbReference type="OMA" id="NHWTIRS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="317" mass="37398" checksum="E18C7EF2989A4C8D" modified="2022-10-12" version="1">MELGSSPLLISFRPVAAPTRYLAACTNKSRALQDDFDVRCVQEKSNDTYFIIQQVKENVYLIKSAAKPNHYLFCSNDNTRKHEDDFDVRTHQFQEARNNWIIESHGWSVFTIRSETNPNHFLFVATDKSNNHGKDLDVRTQNKENQNNHWYIVSNPNFTTPYPLKYDPIIVTFNPAHLPQHTLTIGDSHQSFTDDFSVNVKQNNNQHSKFILDRVNGDAFAIRSFLFPSFYLYVDNKKELNQFHDYDVRFHPIKELRNIWLIEQTESNHWTIRSLTNPNQFLFAVQDEPQNNQSFPIRTHPFQEDRNQWIIDGLLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q411</accession>
    <name>A0A8S1Q411_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1400128</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q411"/>
    <dbReference type="OMA" id="QFQESKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000334">
        <property type="entry name" value="Heat shock factor protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="3">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8109408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="246" mass="29173" checksum="0C2FE76F32D70DD6" modified="2022-10-12" version="1">MNTRTPIPTFILKTYQMLEEQNHSNIVSWTAQGTAFIVYNQQQMEKQVLQNFFKHSNYSSFVRQLNLYNFKKVRSNEGQIFKHKCFRKGMKSMLQFIKRRNQEDLITPAIQEEPTIQVKEEQNLFKECAIDIKETNNKLKEDMKLLQETSSYLIDQMQNLNHFVYNQSVDIEVKFKQVGQMLHAINEELRQENKSETQTNKFLGEYKSIKDDQFQESKIGSPNPYVDYNSTALNPLDYECFIDSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q448</accession>
    <name>A0A8S1Q448_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1420119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000146">
        <property type="protein sequence ID" value="CAD8110007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q448"/>
    <dbReference type="OMA" id="MIRMIKN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="469" mass="55145" checksum="E87661B197B8E409" modified="2022-10-12" version="1">MNNCSNKRNVIFKPTLPRLKIIQQKRQILSSPQKSKFNDEKKLEQIVEKRNFQKQLVDLVIPIHDDAQSSIINQEFSKNDLLKHYKQLPSKPTLLFEQAMKGLHPLQQQEYRNKELKKLDKKVQQLNERNPLQESWKSPFLSQPRLYKSSEEIDLSASQEIDPNVQNVVVLNENLRGFGSRLRSYLDYDKNTVFERQKGNPKYLYRDNMIRMIKNNTTKQLDLVKEWNILINNDKLVSHAQLQKPEIRMQQFGTISSISHYYSIQDFHKKKMGQSLTQQTPNSQISPQFVVKNSRQSNSSEPIDFGKSNQVLLDVSEVEKGQDDVSIHKDTFLTYCQVFWDLIRKEDEIVERVVWAFCKGDLFTFSGFKHFYKLIVFQEGTFEDYIKFTYDFFLASDRNEIPFTEIQGLLRLLASRIDGKDHVLSDQILMDIVSQIHRPQVISKETLQQLIFEDKIPPTVVVKMVYEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8J8</accession>
    <name>A0A8S1Q8J8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1490082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8J8"/>
    <dbReference type="OMA" id="YRNTIEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="481"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8111872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="998" mass="118661" checksum="DBD3DE5D62B4BB45" modified="2022-10-12" version="1">MSDQDSGSNFPQPLQIKNQTEIADRTYSEILMEQIVNDDDFLYQSMIDDDQYSRPKVIPTIANQTIIYRNTIEMKRQQPNIFQRCFGGKKSSNKVTMAEIIDLRQKELFNDYVYETEKDGFLKDVQKYIHKVKVLEPDSQFYIFWQVLNSILVILFFFQIPFIFAYQPLIQKNKSIDYQFFETFALNFAIAIFVIDIALTFNIAYYKQGYLVSGRKQIAINYIKTYLILDLIPLFCLIEFRIFLHQKNQQFGIEIFLFILKIYEVFKTSNMIQEYLQLEPSKLAKYRLLTVMLTITWLCHLFACVFFFVGRRELNKGNDSVSWLSESDLITKHGGYTTLQEHIFELHLYSFYWAVTTMISVGYGDVTPKNAWEVVVTVVTQFISCIVFAYSVNAIWEMINQQNENKQKFQKYVNAIERFMREHNVDRKLKARIEAYFYHLWESEKARDHELEQAMILKLAPALKEELIYQTLGKMLNHNQFFSYFQQDLLIELAQDIQQQYYSQEEVIFQEREEIDDFPVFFLTKGSVEIYLDSEKPIKLHIMKQGIFGIVAFITGYKRTASARCLTYSVIYKLSRIQFLKRIEKYSIEKQKFFEIRHQVLFNNNYVKYQLKCYICESKKHLAINCKKTLYIPEKMPIILETYHLQNQRDSIYQRRVVKQQFKALSHIREVQFNAVAIKKYFQNITFTQNSSDEDIEYSSDVDDYEEEMEDIKKIVEKEKEKAQLRRRGQWIADDDYEMKIDESKDNHSIELSDGFKKSQHLIKHSDEEIKKKSNVNKKKKFQSFIKLEKFRNVRTSSMSSLTPIQEQVKKPFPIKKDKGMSALLPNNLQAQRKKSKLLLQNEIQSFDKSQQLHQQNDKLDKVQSPDRNIYTESKKSELLRKMKFLQIAQPKNKEIQSIIDELKNYIQNSQNLQGSISKEIGSALKVEKINNNNTNNNNNFQQMETHSDVDSQFSIDHMANFDAYFQEENPEKVIKKYKKQNKDKLKQRRTTMMRQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKN0</accession>
    <name>A0A8S1QKN0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1700041</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000179">
        <property type="protein sequence ID" value="CAD8116253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKN0"/>
    <dbReference type="OMA" id="YMEAKIP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF39">
        <property type="entry name" value="SECRETORY 24AB, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="4">
        <location>
            <begin position="104"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="5">
        <location>
            <begin position="172"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="6">
        <location>
            <begin position="399"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8116253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="743" mass="86989" checksum="954CE9B8D178A266" modified="2022-10-12" version="1">MSVHRKILEKSGRLDSLQQQQQPEPYAQELGKQTIEKQDSEVLKNLDAYQQYNSINNIRITSRVFPRTKQLYMEAKIPFAVIIQPYGLSTKQGFPTVNYGNNPILRCQNCKAYINPFMEQLKDEDYLRCNICTCIIKIPKNFIKPEDLEQRPDLINGSYDIRAGQEYQMRPPMAPAYFFMIDVSYKSQEILGIMGQIIKDMILEDKFNQRTLFGFITFDTSIHLYNFNSKLKQVQMYVLTDDNEIPTPDDYLFNLQDSKDIIVAFLNQLDKLFPKPQLKSTQFIQAFNLASKIMQDNGGKLIMLSSSPIKELNNTENSKSPQYHFQPTNNILKQITEKMHLNYICPSIFIIPCGFNNICTLNQLVKFLNGDIFFYDDPTIYTQKFYYDFRAILDRDYTWESVFRIRSSIGWKIKSVYGNYSIKNADLLNVNCTDDQKVLMYELELNQPRAPYDNLYIQTALLYTSTQGERRIRVHNFCIPISNSIKTIYSQIDQSCLAISLFKIALSQLNIAKDVKDCIQTKDFLITATRSISNNCKIFKEPLDSLHSYMLGIMKSQILNYTYDQQSVLTDYMNYLRSIFQYLNVDELVTYIVPQLYNISNLQEQDCIYDDSGYFIYPQPISLVLEEVSNGGLLLMDCGYCLILFICKIHDENQMADIFGKQYYSINLIEDNLYSNSKNPINDKLHYLITELRNNKYTKYAPLYIVKQGCKSYWEEVFFQNLIYDDFNKNYRMGYNQFVQYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QL27</accession>
    <name>A0A8S1QL27_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1640013</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000171">
        <property type="protein sequence ID" value="CAD8115367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="LCMKYTY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2075"/>
            <end position="2100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2120"/>
            <end position="2139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2175"/>
            <end position="2196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2239"/>
            <end position="2265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2277"/>
            <end position="2299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2327"/>
            <end position="2345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2351"/>
            <end position="2370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2382"/>
            <end position="2402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2414"/>
            <end position="2435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8115367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2466" mass="282752" checksum="CF749D5C09E3BAAE" modified="2022-10-12" version="1">MLEVKMNVKALVFVFGIMICVFQIPVNHTIKRQIIRIHARITHKRIVENKSSVAFILVNVLTSQIATYLIKIIVKNRHINVYLMVQSVFRCQNVVIIKQRMDVRIKIRMVIIAFGLWVQRKNLPIYLKSHSMCNEGLNGCTISEQGYGCIEQMESCSQYVNYFQCFESKQRKDNCFWDTKNAKCVEKVCENLPFTQDYECKSYLSDCTTNGIHCVRRKQCSDAQNKLGCISDAQGNKCEYHENQCKNKSCDTAPNSLSNYQQCQDYDNLLDCVTSEIKGCKNRPVTCNGYAFEIDCYSIQKQDCVWYENKCEQRQCYHAPIYYSHIDCHQYGNCIGKLNGGCQITPQSCEDILQEQFCELNYNKEKCIWLGGKCVLLECNKLKLPYYKNHKICQQASQFCTFNLDSIGCTDYLCENVSEIEYCKIDSSGTVCTLNQGCIQKQCKTAPPSYDTNQKCEGWMPKCTVNVQFVQNQKILIGCVDKKISCQLAIQDQCYTTISGFKCKWDGSSQSCIDQECTDADPKVYLTNDDCQQFQVFIGPCIIGSSGIGCTKWPTNCNQMINQQQCKLNLKDGTKCFWTGTYCKKQQCSDAPPINYTNNIECNTWFNNCIFNHSQGGCKDRPISLACTSSPNNNMYDTHQECFAWNPKCTVTSSYSVEGCEQKKDSCYEFIRQRNCKTTLNGQICYWDDKLQMCMNEDENNNGVADCDKRLYGDLTHQDCEEFMPKCTLNNIVRSCQYLSYYCDYQYKQQCQITRYQQPCKWDDLNQRCKNVVCTDNTTAQTEVECLKFKQQNECQLKIKSNGTYGIGCENRPTTCNSITDPVICKLTLTTNQHRCYYFNSICQVVQSNQCEVITESKSDEFCQLYNTQCVLQSSGQGCYSMVNCSDLSNKICNSAIMRYNQKCSFQDICRWDYDCSQKYLSSSHCNDQKTQFGQLCQYIYQCPYYCNYYCRLQTTQKNLTFSSSATLLDKYKQCQVYSSSYRYDTNCNCCVILTSCNSQIGSEPLCNLSIDQSRRQCGYNQQTQTCEDRKCEHLTSSQPISRQLCFEWKYNCVYDATGCKTFSGDCTTIGFIQQCYSFSCQWQDGKCVNYVNCELNTTAVTNRECLLINSSCCRLNYNKGQGCAFTNCIQITSSTICNSAQLANGERCRWDNYYSKCYNKYCQEYAIQSDCESSYGYISPIATKCYWCSNYSTKCTNNSYCNSSSMTSYKSHQDCNSVNVLTTIQFIISAKCTVKLQRCSDYKYQEACKKTIDGLECYWYSNTCLNICEAAFYLISSHTNSSCHSWHASCMQLNSSECQTLDCNLLTTSANCAIFTEKCFWDGSNCQSIGDCSKYSTNTLCLNTSNSQDIPCFWNGTLCLEKTCSNKPTLSLNQSECDSWLLNCQWNVNNNQCVQDCTQANISNNTHQLCEAYYLNKSCTVKLDLIQCVDLPFSCPLAKKTQCYIDQSGNECYFQVSSGQCVNLLCSNLPSSYKKHETCNQRLKSCTVNTTLNGCQQLTDCSNYSIQDQCQIDSNKIECEWIISENKCTIKECSTAQLPIYSAHSCQQYFGDLCTVNANQDGCEIGQALCMKYTYNQCKSDGQMNLSGVDCFWNEEKSICLERICENGPPLAQSHSDCVGFLSTCQKGGCRIKGCFDYNYAIDTACASIFEDKRCVTNGYQCVLRNNCEDVKIIDGCTFDINLNPCVWINEKCQIKTCGTASVSLNKYLECNEYFPNCTVKQGGGCTKKQTCENYQIKEACFTDSENVECIWDDYLNQCFSNQCMDFCGDGVVSSKEEECDDGNYLPYDGCYKCQVQCPQGCNVCNGRICEDCQKNGWLLINGVCTSKCGDGYTVGSEQCDDGNYIEFDGCYQCSFSCHQKCLNCFQGLCLQCEFGYLEDGSQCHNICGDGNLIQQIEQCDDGNQQNNDGCSNTCEVEKNWKCHQENNISQCKYLILPMITLTKLTKTNSDNQEFQLSFSEKVRLNVNGISEEQFLQMIVVVLENANHNEYDVEIKPIISITTQLTDVAYKILVYFKSKIQNPLLKVTILCENIVNIQDNSLYSNEVKLVLKSPNKMSNDQKSLIQKTAFLSRIVMYIILIVSGIAFLFGNLEILWNLLDMLQQLSYMKFHNLQFPENLKIYFEIFSIGSFTPIINFFQTDIYLQNLFDYQIPIIPAKWKFEEYEINCYFLNNFQTLIILIIIGFAYFLVSFFFYKFLILIKYQNWPTIFYKSKSQSLFKFVRLIFSFQKLARKQCSYFVYSGLIRILTSNYYELTFASILQIANYNIDTTLNTTILLIALITLQFNLILIVLFFSYLSKKDKVSKKLSVLVEGINNHFSQGSKQYVTILLIKKTLFLFNLIVLQELLGAQSLITACLSGVFSCYIYIYKPFENNYENLKIFITEIMIMLNVILFSVYDIIKFNQNKDSAEVLGWINVGGFTLILIFTLLIDIYQQLLKYIRQLRKNFINQKKNMKSKNFIFFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QRY0</accession>
    <name>A0A8S1QRY0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD-40 repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T2060008</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000215">
        <property type="protein sequence ID" value="CAD8117665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QRY0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="395"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="437"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="479"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="521"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="563"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="613"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="646"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="688"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="730"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="772"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="814"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="856"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="898"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="940"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="69"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8117665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1106" mass="124900" checksum="74E02F69C583EFD0" modified="2022-10-12" version="1">MNCTQHIQNPITYFCIAPHNCQCQRKLCIDCLYEHKVDIKYTLSSNRFREKLDQTCELTEQRMNFKSLLTQIESLLEKILEELSETIKQIQNMLKMQNKSHLNIINENTNLAELQSSDLERLVQIVQETTLDDWNTQKNSYLYKLEKAKNWWGQEIKGFNEKIIQEVKEILSLETESLFKLQPSQDEEQVYEWKEDLYDLLINLKDIDVNILNLILEMFRKEKIQDSLEFQSNHVIQKLFRLQILNSSMMNVEKNNIKYIANVIKNIYALDFNKQNYSTEIFQIARKNLIQKISKDKKIIELLKFLVHLSAINEQFIQCGSNALNLLVEMKVDLTNQSFENIRIQNTSLIGANFFKCNLSGTVFNNVDISGMNLNCAQLFNCKWTNIKIHELNKLDGHDGAVSSVCFSPDGTTLASGSWDNSIFIWDVKTGQKKSQLEGHSNFIQSVCFSPDGTKLASGSWDKSIRIWDVKTGQQKFKLEGHSTYVQSVCFSPNGNTLASGSWDKSIRIWDVKTGQQTARLDGHNNPILSVCFSPSGMILATGGYGTTIRLWDFKTGQQIGKLVGQSSVVWSICFSLDGTKLASSSEDKFIRLWNVKIRKQTSKLEGHNTFSSLCFSPDGYTLASGGDDYSIYLWDVKTGQQKAKLVGHKSYVYSVIFSNNGTILASGTDDATIRLWDVEIGQSKTKLDGHASYVYSVCFSPDGTLLASCSEDKSVCLWDVKTGQKKAQLNGHDNSIISVCFSPSGTTLASSSYDTSIRLWDVETGEQIVKFDGHANYVNSVCFSHDGTTLASGGEDQSVCLWDVKTGQQKAKLEGHCDYVRQVSFSPDDAILASASDDKSIRLWDVKTGKQIAKLDGHSDYVRSVSFSFDGFTLASGSWDKSVRLWDVKTKQQKAILEGHTNFVQSVCFSPDSNTVASGSNDNSIHLWDVQTAQLKASLEGHTRNVYSVCFSPNGTKLASGSWDNSIRIWDIQTVYQTANSNHHSDYVRQVDFSTDGTKLASCIHDSSICPQDFNRGNEIELTVGNYRDIPTQFKQKFTQKNLHQEIILNVPILIISQYPYLLANGALIFQGEFVTSCNEDLQILLKKRGSMIYESLIELEKKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNJ1</accession>
    <name>A0A8S1JNJ1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0060104</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNJ1"/>
    <dbReference type="OMA" id="IFIKYPQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="65" mass="7929" checksum="185B41A0B940DCA9" modified="2022-10-12" version="1">MIENVSKMIKLDKVHLHLWLIKIKNIHENEKDNHQLNGTFQKYLIIHYFFNDISTLKLEILIDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQR2</accession>
    <name>A0A8S1JQR2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arrestin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080472</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQR2"/>
    <dbReference type="OMA" id="CVTMPIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000024">
        <property type="entry name" value="Down syndrome critical region protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028934">
        <property type="entry name" value="Vps26-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12233">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 26 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03643">
        <property type="entry name" value="Vps26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="35874" checksum="8DC970EE1BDE70B6" modified="2022-10-12" version="1">MKSEYILQIKFDKANNTFKPGDFISGGVTVVFTDPTMKRLEIQQFIWKSEGTIISQNKETHKDLERMSSQLHPVLLHQLDGQLTKDKYILQEKNTFPFKYQLTPYQDRKILETYVGVYVAIQYSIETELTLSNGLKVTSTIPYFVYVPGQGSDRIKPTDKYPRVEHFLITPDKLLGNSSTMNKSQNAKFRINGLIDTSICQFQEDFHGSLIIEECDSEIRTIDLQLIRVEKLENNLGKISEATEIQLIQIVEGNATRGLEIPFHMIFPKFFSCPNFQFREFSVDFEVNLVMILYDGFKVTLNFPINIIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTD5</accession>
    <name>A0A8S1JTD5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dpy-30 motif protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTD5"/>
    <dbReference type="OMA" id="DFNEYWP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22967">
        <property type="entry name" value="DD_AK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047499">
        <property type="entry name" value="DD_AK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007858">
        <property type="entry name" value="Dpy-30_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05186">
        <property type="entry name" value="Dpy-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="758" mass="90162" checksum="825B584BB6678A9B" modified="2022-10-12" version="1">MSKRYFINNLDSPLGQEFYSQLVKEDTEEGVHMATYTIDDPNKMPPKGFKKILKRYKPKLSRKKMLEECDVYVYDVASATQTDLDFVCDIFRNSKTTLEEQKVLILVSSVLTWSAMPDKVKPKVEKKVDEEQEGEQQEQQEQQQKKEEAEAEENQEPKEDENNQDQEQDQQQIQQQQINEVPEIIEYVPWEESDYKLRSASTQYQQLKEWEDMVLGLQKENLKVIVVCSGLIYGKGEILFQKYFKKAWLQQPYKLSYQSDGNNKVPTVHITDLVKMIVKVSESIPESNYIFAVDNTQDRTQKAIIQGIATGVGSGWIEQNDDFFKDFTPEEMDCFRLHLDCKTSQMFIGNEETPSDFEWHCEKGIAFNAKKILQEFTSIHKLRPIKIIMESKAENMRFQDILNMISDHYRIPVINQDQIFKDAQDPNYVFPSEIQEDFNEYWPVIQEYINTKAPAQLSEQLKIYYKVIKWRLSQNDCQNRGFILQNFPNFFEEADFIFYPNKQKLKMKKKPKKKPVVTEEEKKQPQEPVVDENGDPIEQEQAEEQQQEEQQQEEQQEEEQQEEEQQEEEQEGGPKPEDFFPESYIVIKPLGKTDNFDYNRKLINFFSERNLDAYFIDPRKKTNHDTFEDLRIYIERNGRPYNYLQNEKELIVLYFNLQKIKDLTIKYTRKQSIQIWKEITKVKKDKRQDLVTKYTKYIQEQEQELQQVKQFPFRNYLMDFVVPVLNEGLVEVAHILPDDPVEFLAEYLYKRSFNVDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVS7</accession>
    <name>A0A8S1JVS7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0100477</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVS7"/>
    <dbReference type="OMA" id="LVYKCAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001605">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="288"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8046581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="673" mass="78850" checksum="F3110F0F389BDFC0" modified="2022-10-12" version="1">MINSNEVSPVKMSNEQKQDIMRSILKEESMINITLKPIEQEVFLAEDPQVALLKLKCFQELINKERLAYAEHLAEVFEMIGDDAYRNLGFILKQFVHDSYSIQLSIIRQLPQLVEQIKKRQSGYVELKDHVLPFMNIFLVHEDSDIKKEVLTQLLKIAPFLIESDKVDTILAHLIEMAHDEKNHQNRILAAETFGQTASLFSMKYCESYICGELLSLGIDLNEFVREKAIEQLPNLSVSLSQKCIELKIIPFFLQSCEDRLLQIKFACIKSISGISKYTGHHLKTQKLIPKYLDFLSDQNKEVRKTAYEYLGEFISTIKFTEKDQQQMDILLAYYQNQLDKNINMMGVDLVYKCAYYFPGVLQAIGRNRWLLIQPLYGFLLKYPSDKVKISLAYSFHEVAKLLGPDLSKQYLITVLETLAQDKLQEVRNGIVVNLSKFISIFPIEERDYVIDLLLNFQKDQHKWRTRQIICTSIFPITRCFNNDTIFFHIAPILFKFCSDTVAKVRRKASKNVIHLIETFEEEGEQRTVILEQIRAFSHSKRFNQRQSYCWMCYKLFYRQDFEDLFFERLFELCKDPVQCVRLSAVIVIDKFIQKEGFSKERKYLKYLLVQFIDDQCKEVQLILTKYNFVIQKTKKKTLSCRSRNSGSSNGHLEDIPEFDEKIEQIRKSSGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXI9</accession>
    <name>A0A8S1JXI9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0110446</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXI9"/>
    <dbReference type="OMA" id="DQPHAIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="109" mass="12213" checksum="E93F23824F714084" modified="2022-10-12" version="1">MNQLMNQGTEANQGRSDQPHAISVNVVVNLQQANLNLQLSIDPEIPFNVFVNGIKEQIFAQRMQAQYEETIEYQLGNGSNLQQSETRTLQALGFTNTCTLYVRLKLKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K080</accession>
    <name>A0A8S1K080_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0100338</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K080"/>
    <dbReference type="OMA" id="WIETFDA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39867">
        <property type="entry name" value="HELICASE ATP-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39867:SF1">
        <property type="entry name" value="HELICASE ATP-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="217"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8046410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="893" mass="106325" checksum="DB54A39EAA8D0B48" modified="2022-10-12" version="1">MKKSQSQNNSGAFDFPDVNKPRFEPQLHKISASTQQKVVIQKLTNSLNNHKIITQTTEQPQTIRSQVSKPLISSERFMFDLDASMVYIHREKIMQFEELEQQRIQQLLRDIQIKVPQINDKVNHFIALAGQFENEKVNSLVNQFIKVVEQLLQIFDEKTKEIKEYQFALKQSREQGQNNSRLIEDQSQMIKYMESQNQNAKNVTRTFQRSNELAVINVQLEKKVELLNQNLQFLMNQNKDSLATQLQQKIDQLNKTIEEQQNKLYDDNNKQIQLQQQIQTLKSTEFKHQIQLRKLEDKIQEYESKLKELTFQVSYSKEQKDQYRELYYMTYEDLQMNKLNTQTERKQMQKSFEKIRMLQDRIDEMTVKRSKEVDAPFSSRVEGDMIQLQAITKILDEDHVFQRYMMSSFLKNVQEYDSKLVDAYVDVKRLVVGRDEEAELQTLRYNYPPFTLFLEKRIVDQETQYLQIRPISNNFMGLLRAILDGMYIEFQKNTYISFQSYIISWLSTFTIENHQVIIFPEDTKRVKIEEQLHTFYLDLMVPRLDKVWEVVQFRHFLNDIYSLEELYFYLHVRYLLRRGPWIETFDAIYGVVHYMKVQQAESIILRCLEQFDKINQTLVIRAVNERIVEGKNDRKLIQTGFVLQLLLEIFRIDRINRYKILSFAFPAQPINFKQFHKFVKGNFSKCTEGEVAELWREVYMISNGIPNLNSFFTAAQSLFIKSLILPAIYTVPLLDTNKKLAFDELNFTTMKIQEQLKEITDSDLDVLKTLIPQMFQEEMIKITKQLMPKNQIQFVNPIEYIFKFMKLILTLKYNTILADGLEEDGVNRKGFITETFTQFITAEINELNKVIYKKQSYMSDQEIRISRLQKYAKKKVKKIYVIMSSVLQSRIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K325</accession>
    <name>A0A8S1K325_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FGFR1 oncogene partner (FOP) N-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0130219</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K325"/>
    <dbReference type="OMA" id="HIEPVER"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034453">
        <property type="term" value="P:microtubule anchoring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018993">
        <property type="entry name" value="FOP_dimerisation-dom_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431">
        <property type="entry name" value="FGFR1 ONCOGENE PARTNER/LISH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431:SF4">
        <property type="entry name" value="PROTEIN TONNEAU 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09398">
        <property type="entry name" value="FOP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FGFR1 oncogene partner (FOP) N-terminal dimerisation" evidence="4">
        <location>
            <begin position="47"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="173"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="173"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="304"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8049081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="367" mass="44563" checksum="81DF085DAED5DB9B" modified="2022-10-12" version="1">MQELTELKNLVIQSLEANGSLAKIRAQIRASVFNVVDQQEGNNKKPSPFFWENNKAQTIYELGCGRDMLELIKEFLLFFEMHYTNSIFSSESNLREDINRDQIAKKLNIDATDSTKPLLYFLLKNRQPEKKSEEKGQQQKIAQPSPKEIQLQQQQQQQQQQQQQQQQQKLQDQKLQEQKRQDELKKQEELKKQEEQRKQEEQRKQDELRRQQQEEQKKLEEQKKEQQRKQQEEQRKMEELKKQQEQQQKQEQQKQEQQRKEQEKLKEQQKQQELLKQQQEREKLEAEQRKGQAKKFEHRNYEDEKFDNDDLEEQLEGEDLRDSQLQQDQFQESDEYMQQSQSQGIDMTVDSAALEEFDYYEDIEDMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4K1</accession>
    <name>A0A8S1K4K1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2HC/C3H-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0110317</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4K1"/>
    <dbReference type="OMA" id="NPKYGRV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026319">
        <property type="entry name" value="ZC2HC1A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049899">
        <property type="entry name" value="Znf_C2HC_C3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555">
        <property type="entry name" value="C2H2 ZINC FINGER CGI-62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555:SF5">
        <property type="entry name" value="ZINC-FINGER OF A C2HC-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13913">
        <property type="entry name" value="zf-C2HC_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52027">
        <property type="entry name" value="ZF_C2HC_C3H"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="162"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="240"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="282"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="472"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="558"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="316"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="429"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="594"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="653"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="337"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="602"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="653"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="665"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="689"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="709"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="738"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8047216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="766" mass="88693" checksum="CA37F7732E9067C8" modified="2022-10-12" version="1">MQGLNNQNQNPIVRPKTLICYICGREYGTKSLEIHLKTCQEKFLMEEAKKPKNQRRPLPQPPKAIGGSGNYDTESYNDQAFHAYKEQSLEKCAYCGRTFNRESYPIHLKMCKADKPFKPLPGFVQNPKEVQQQINRDNGGTQQGKSNSNGYSGEYKAPVRPKTLVCYICGREYGTKSLGIHIPQCKEMFIVQEMKKPKAERRNPPTTPRGLWDLLNKEDITTEDIMAYNNGAFNKYNKEALVACKNCGRTFKPEALEHHIKACTVDNPFKALDAVEEGGTVELVPCKKCGRKFLPSRVEKHESNCKDIKGAAQTQANGFQKQQEQKQEQKQILETPQQNQKQQTKQVQKQQSFSKTSVNFNDKSLKCYKCFQDCNNIQDLKQHVLNCKNEGTKPQIYDDLIQIPNPSQALQTIYMSSVFENFQQKDNNLTTPEQQKSQPKQQSQPQKQQSIQQQNNNHFSDEDQEDKEDEIDLITCEKCERRFAQDRIKKHQKVCKGKQYFEKKEHKVEVQKAPETGWRKYHEEFINTIKYNRQLKKIQEEGGDIKQLGPPPVSLNSNYIQCPYCQRKFDPSKADKHISICQNVVNKPKTIQEKKQNPLHSQQPQQPIFQLQQQQQPQQNFKQQQKVQQTNQQQQNTQNPKYGRVFQINDEPSQPKVQSQQMNVQQSKSSQQRQSISSQKTPQITQKATSQQQSLLSPPQTTNQFRIRSPQTTQKTQQVKQSQNSTKIENNIFRPPTSGRTQELTKPNSQLQLKPISQNQNMFRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7U8</accession>
    <name>A0A8S1K7U8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7U8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="237" mass="27316" checksum="6270E19B65B74AA2" modified="2022-10-12" version="1">MLDLQAKTNKEIWQAKKLLYESDGNTKKSLIVNLVALGLLCILLFYSIVGSDDKWTCEIESANNRGHYICVEMRRTMNCKTISGPIRSEIFLKVKKDLQASAIIECPWEIAISEIRLCFILLSISSVLIGIYALRKTNKKYAELSFQIGISFAILLLISSYFDYVSIRTSQINNYNLCNLQEEFQIEEKMKGQMECSFSFYNFTVILEIACSVAMIVNSIYINQWRYKQITELNDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K843</accession>
    <name>A0A8S1K843_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0170333</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34969">
        <property type="entry name" value="OS01G0621700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34969:SF1">
        <property type="entry name" value="TH1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="64"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="229"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8050987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="342" mass="40486" checksum="74A575741A20B1DF" modified="2022-10-12" version="1">MNLVILKKNIVDQKIINILKKLQKKIEQQQNFQIQYKNYTHPKNLNEIGNGLYDGSGKQASFKVSQKQGFLQMQQKTLFGFQDWVEFFALMTDVGLLLFKSAGDMHPILFVPCADAIIIKNPLKCKPNCLKIKYRDYEYIFNVTSQALLEEWYQELNRQVQKLDNHIPVAIQILNLRIIFETSLGYFTPILFXKEIETSMKILLQLEEYTEQENIMQIAKLLITQSEVIKEQQINIDEVEILCKDLQEQIDNLCIHKQQLINENQKYSSQIYELQKNYNSIKKQLDSEEYHNQQLSQLESHIKTLQLELYFLNNYNTYLISENKRLNENNQQLLLIIDDHEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8G7</accession>
    <name>A0A8S1K8G7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PSI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0170374</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8051067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CEIFNTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035936752" description="PSI domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="3445"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="21"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="913"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="1476"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="1801"/>
            <end position="1846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8051067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3445" mass="399269" checksum="6C1CFA89D71C8A0F" modified="2022-10-12" version="1">MIAKTLFVISFLSFGVLSHQKCNMYPHSAQKCIQNTEKIRCEYDNFGHVCSNNMETNLGCSPNLNKEACLNQLTDGYNNRIQCIFQSKCKTVEKIQLQKYGCYEMFNKNSCLNVQKHSCIWQDNRCQPLKQTIQDNSKICNIFTAPVTPFTCAQLPSGLCMSSGYEGDFQCVELTLDQLSKLSCYELGLNEEACLAINKENQYCIFKDNECMEIKLPDIKSCDNKFSQSLCLSIQNPDIQCEWVDNKCIKFEKNSEFQCVTYNLVNVSVCQIQNGFCQYNVRNHKCMNIEKKLIHLISCDTPGLSRLACLSVQNYNCTFINGRCQELSEFELNFFQCHMELNKQACVNILTPFQYCFWNGNNCERRIINQDYDCPLKFDNNNFIVNGNVCQAISKNNIKCKYNKQTHLCIESSINDICESSFINRFGCLSIIKSDQTCQWTSLGCKNIEVIQYITTCASLGSANPIACSQVHEKEENIGCYFDYIKQKCVTLTIRLNPNANKNEYQQYQQDLETLRNIQCNQIQQGINRITCSSITTKDTACMWKNNQCIQIENMKDIQNIPCLHLQYANYGACSYVQYGGEVCRYNSDIKGCVNSISPHMQCNEPGLNSSGCLKAEGKCQFINNQCQNPDKQTEENNNEIIEIPKPEPSQTCGSTSPTKAVCVSFTATQLICQWSKSKGFCEAVEIKENQYCLDFAKPDIQVNSNVCASILSDFPDYDYQKGNQVDKNRGYCYYNKQTSICQIKKELCNTNCCTENENIGINAHSCSKFSSTEIGVYCYFMNLRCQELTFETVDITNPNEVKQYYNEIKLPCSSMNKNSCHMINWSPEQQCYYNGIICNQFNQKYHNNYRIFTEEPYILNENACIAIDGQITEFNTLKYIGYDDINNRCQELLLDPEYSYNYCEQVKGNRNICQRYTGYSYCKWDEKQLKCITIPYDEYDEIQSCDSELNVRACTDLKNISCLFSYHLDKCIKFNDVNVDCNHFQTIGKVSEQVCVQINKPGQQCQFHNYQCIDSTLTYYRCKGSSANNRSCYANTKFLCRWDSNSLQCYENYTKIEELSCLDNINIELCKKITKEACVWNDILKQCEIFNTINSIDFETFNNTGNHKFNEIACLLITGDAYYYDQYQQKCLKLTSKTIDCNTYQMNKYACLYHTQTHNCYYDEQEILPNNKCKPFIQDQSICQTQWQINIEVCMNIYNPCIFDVATLQCQSFEFLDTMTCTDLSNYSINLQHPNKRVCSSVVQSLEETLGELLCVEDKVNTQECKFQKYCFWSNYSCEIYQIVYNHLDSKSGQEQSQKECPEFQKVDTCFEEITKNESYGKNIQQLIENNWVFINETCNMQIIKYTRNHTYYQLSDNTNVCKIQECSIIERIYCYVITIIETIIPINEITPDNYDASIHDIKVITDNYDECQYFYCGQFTDYDCNIEKAKCQRKKTCKKDITYPHDQYINNDCTIENKYITNHWCLLEENYIINCISHFSKAQCLELSPKCFYDANQGGCMQLEGNEHNIGDCNQIANDCYVSQSHNAICQNGESTIQPGDKCKTVQKEYKYCVPLTTYQSPYFCSDILETDAQPILCARADDDCRYDGKACINSMPKLKGSGSYPCDKSFSKSICEKCGCNYTYLGYCQYYRQEPQLNQDKSNKDFLCYQVNLLDTIYKQEVCTKVDQACAFLDNNCLDATHYSCNQLFELPVSLKACMKCQEHATKYDDIQQKCLNIDNIPNQNTCSNLNQLACLRKTEGVQCKWENFKCNTISIFESDKIECSILNQTACYIKQINICWVDQTTFQCVNYDPYQGKCDLLKTQSLCVRSLYENCKWTLNKCIQNDEITTNTCNNLNQYQCLNAIDIACGWSDLYENCYELQFRYQPYSCKEFLIDYQYQYINRNNYFTCTQIKYKGGCIHDQYYKCREVIPTDILSCEESIISNINEYACIRLTKGYCKFVDNKCQKTQESNLGCQSYLNQQACLLQDFACKFDNYCQSYVIYHTSGIRVYFPYTEKVCQSADFYIYGQVGIGLIYSVAQQKCIDITNKNMMINDCNYVGMNKYTCLLKTNTYCEFYDNQCRNIQMKVISQLKTCKSTLNQFTCTKLNVQCKFYNNQCVPITDKDNCHTLQYEKSIVNLRICLNDHETPCMFNDKTQNCEIITTPQKCIGLNYKGCIFFTQGSYCEFKNSKCITSFGSPNCKADINQDKCLSIITRGQLCYFDKKLGCQNIDTTKIDINKCYKSGLQTNPYTCSRSIDIPCFYDKQNKQCTRYTEQTAYSYQQNYSISNISSFNKWTCQMYNIQKALIWKESCLVVKSSELVYIKCSDLVNKIACLSIQTPYQFCQYKNNKCQNANLDDFKNMSCDQIIDINSGVFCALNQNKIPCEFNKSLFRCQIVVSESISCVEKDPEEKGYNQTCCEKDKNNCTFDENCYRLNYSGFQFCKDIKVDGQYQCNQVTGEGCMIQQNICKKIYYQDYSNITCEQASNRFGCVNIQTQDQYCQFDGEQCKQQDLVQYKDISCLSIVNINNYKFCEQVSDIACTYDLKKKECRNVYQQEEFSCERGLNKIACLNQTIKGLQCKFLEYCYGPNNGIMNCSYNDKEQCCREADSIDTCLNQKIYECEWTNNLCQASKNTLNECDQITNGSFLVCASIKNTLCVFIPTLYKCESQKPSTCGLSQTSQQCKRMKSISCIWNLQEEICNYSEQSAYLSCQQINERSGNQRSCMSVEVPGQMCIFKDEQCLLFIQQEDTNNCLDNINRNACIYQTISDCQWINQVYKVNKYQNSDEEEIVQGECMTITNINKMSCSSNLSYTACLKITKIDQYCIWKDLKCQTIDYQQQYTPNQLIYVNLNACGLVNNGDIVKYDQKQKKCVIIENVQELTCNPVIFGINEAACLSITTQPCIWNSIEKICQYQDILIESQQCDKPHWNAKSCSQLNINQPCGYYINGCQFVDINTIKCTHSGLNKFACLNIKQYPCIWKQDNNNYYYCDDYIPYTSCELVPINVNLKVCQLVDLDACYYDINNNKCENNNLSKMNCDTLGLNIIACIEIDGCVFQEYCQNVNKQIYTCNEYSIANYKVCMNAIDSCKFNELTYGCIQANEELCDTKGLSQQGCNSSKCTLFNNQCLCNSIKDKDCNYITNIEQCNSLSHCIYLKQLNQCKWKQCEDQSYNNCDGLYINSQYCYSNNLQQCKSAKQCDDIHNPFNQKCPSINNEPCIYNSKLNQCQQLICEILNQQECLNYPYYCKYEQSCTFIGCNSIQKNNCDSNICYWNSDMNSCLEQIECSQILQQNKCKQQKYNNNNCQWVNYEQQSFCTLTPCRYLPNIIQCTGVYMEKGICVLLTDQTCVSCEEIQSSCICLQNNDYCDYNFKEMKCYSKICENYLTKEDCPEEYCEFQETCLPQCNSLQYQQQCDNKQYCIWKDQQCKKKDQYVRANNEEESYGELIQLILLSILILQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAT7</accession>
    <name>A0A8S1KAT7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="223" mass="27127" checksum="55000FC97F721A6A" modified="2022-10-12" version="1" fragment="single">MNLQEQEFFNTFKQCQKMHWIQSKQLSNVKIFYTGTNSFYQRGDIIFDNLIPLNQILDERVQYFRMDKQQQSDGSFIERCKFKNQIYSLKYQYHFFKINESSQGIHRKYDDTIMVQQLVFSKIEKTRTILTIIQHLKSKPNYAFFKFLRECIINYSSLQNAELFEKQSILSELRLDEDEDETNKYVCNYNDTCDYSPKETDEKYINEHFRITQLDQEDQDIYX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KBY5</accession>
    <name>A0A8S1KBY5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0190017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FYVQIGL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035924510" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1309"/>
            <end position="1330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8052107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1346" mass="158560" checksum="84D82DECED36BC99" modified="2022-10-12" version="1">MIIFVFIFVIRACEVLNEDYYLYPTKGEILEIPMEKLFQGNNLIYQCSQCPQDVRIVNATNHKQSIENDYIFTYVTSSQDQIFTLTTEPSLNMYLKLNGSLTLKKVIKIDSNLLCLSVTYIGVNNIILDCYQNETLILYSLKNEMLIQIYTSNSSIPNNTKLSSIIKNKSVYFIYGQFYSTLNQIIMNLTIFQEQQNYLISTYNQQELAYNFVVSQRESTNQTIFIQLLNEIKTYVLSQQGTLLYVTNILQNQKILNMAYYYSEVEYYQFDQIFLIILDDDQDYMYLIVIFYSDSVYIQLLESQQFVKIIDQNAQLFISQQFLIIQDNHKIIIMNNKRFNQIIIRSFDLYQSNSVVYFDQQTQKLYIFGKYIEIYTLLVPTLIFGSNQNNGSFSIDALDIDDKFIIRKCHTIIHYLQVPKLDNNVYFIFQQPQQSYFNLLQYPFIYWINFVSGPLVKVLTLVSNPSLGNFNDSTLRKLNQSLQSDKNYYMVKAFSVNLFQECQSLKLRCFFIVAINNQNLDLYYIAQNLPISSTEISNFNSSVTQIEITFVVLDQQLYFLVCLSFSQNIIQLYQYNRQLQLVNNTTLQTNSFRQFQLLSNAIVLLLDTNQIAIMTFDNQFQILIDSKILNQILGQKIDFDPISIYINQQYQSRILYINNQNSFIIGQITEEFKFVLISLKIVIFPIENLQVVNNLLILSYIHQESKDVQFEVWDVKKLKKPQFLRYMRSLIFKLNKDTISYYSDNQFFYVQIGLIVNVFNPTLSEHSSLYYRINIDGIYFTSTSTDSMTLLYFNSSFFQFSSVLLQTFSNSSIHSDFLSEQIFNFTICSQLYEKNKIYTQNNSVTIINNYQQIQINKTKFDVNQSQSYIEIFRNNISIQGQVLQFTMNKKSKNKNNYTLTNYINYFKQTNSSIIYDLIIQYHDTTFLLMNNSTILNYTNGNKYYFGPYKKCISSTAYNFQIYSLCQDNQSKNYIISMNLTNKINNSQLESKVILTLTDLSFSFNSWIRIKNNQLYIWNNTIFMYSINQDQQKNGSQDFGQFICKSTQFGFQSIQIQENDGTQIIIYFYFKPIHKDQLYYRFAKVGQNQTFNRSDEKSFLFKTYTQQYLSFNTIPMVYQKKKEIIIMVSNSNFILAISFLMVINKSNFIDSHLIFQSFLQTSPPFDKHNFNVTQLPQYEQGLLLFMYSQSINLSNQTYMITFYNISNKQNVNYLTPLFFQGGYNFTLNNTFLSAFSFIARNNSFGQMLLLFTNGSILELQLNIFSINITLSAQRENLDYYELYAQNYFNSAEAEINLVFIKQHIFKTTSFYVLFGITLIVLVISLLLYNVYSKRNQDSQDDEDNYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KER4</accession>
    <name>A0A8S1KER4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0210291</name>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0210292</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KER4"/>
    <dbReference type="OMA" id="CESHTLE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00878">
        <property type="entry name" value="Arf_Arl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="22"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8054088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8054090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="21879" checksum="A2F8F42B3B839061" modified="2022-10-12" version="1">MGCVNSRSVETYQRKFKLVIVGLEGSGKTAIFEYIRQGKFVQTKPTVGVNVDFSHPEFMIFDVGGKVPSLWSNYYENTDGLIFIIDSSNKEQLYQVKEQFVKLNKDLEYMNVVILIMFTKQDNIHLDNQVLIQECGVFGYLEQDTIFQMCSAKTGEGVQEGIQKLINLIKKQQKPNIPLHPIKKHQKKIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEW0</accession>
    <name>A0A8S1KEW0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEW0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="170" mass="20046" checksum="2BA6D27F26A7CD31" modified="2022-10-12" version="1">MHAQEVLMEEKKPQSCIRIRKSLRIICFLGILLVENQVKSKSLVCCWNYQQSQLILQNIQIGIGKKFNSTQMFKDQFGVQMIVQQFLDIMYLKLQDKKISIILLLILETTKLLLVYHNAIKIMSFSTHSDIRGMIQLDHLQQYIIAFVHCEYHRMKNLVTKIFEKNLNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLZ8</accession>
    <name>A0A8S1KLZ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0240221</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0240222</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLZ8"/>
    <dbReference type="OMA" id="YMSIKED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8056260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="356" mass="41819" checksum="6D04C8BA3E3B3530" modified="2022-10-12" version="1">MNTVCQQKTATPCRFETTQSYLLDQSPKIQYRRVNSPGLPDCSFRRSHKSSVDKRNYMTLGQMDIQQLLKPTRSVEESPEQKLKPYYVKGEDILDHHQHSFNPQQYMSVKEDKEIQVEEEPFESPPKMEIFSKPQIRKQMKMVDKIESDKVSVEIFMSEQDENSLIFTGITKRILPSPEENKQILMQNLQQLFTSIDKNANQNKFQNYIKMESLEQSIFHQHLNKSTQQDFLSISSAQSFQQQKCSSQTNFRRNSAIVPKQQSKWNNSTKADKFKNNCFSNKQSKSPFLKVQNQKVSINKPKLQQINQNISSPQKVPTQKKQQLTQQNRSTVQKVRSFHITPEKINDCIYDERFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQD5</accession>
    <name>A0A8S1KQD5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQD5"/>
    <dbReference type="OMA" id="IACQTSF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="151"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="332" mass="39575" checksum="3BFCAE888233E708" modified="2022-10-12" version="1">MNQQSFVQVTQEYDQVKKRVNSLKFQLRDIDQKNFLLQTKLKDLRQISPKFQKSPITQLQEQLGLDRKQIIEHFKPIVEKEGIYLRNERLKTETQLQQSKMWEIQQKKMRVKLIEEFNSEVTKRRAQYQEKKIKEVRQRQLQEMEMNRVILNKKLIEISKLKKQEKELIEEIQSAKTKEQQLCNKFCNALVSNDNSVILPSIQKSNSKFSQNSASKQQRKGYSPILKSSKVIERPQRSETRNENSIIEYEENRKLQLESRKKSQSIDQLNIACQTSFLITDQNIDSIQYSYNYDSYMSNQKDKTMEKSITNITSEETKDQHEKNIQVNKKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KR93</accession>
    <name>A0A8S1KR93_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200332</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="163" mass="19334" checksum="9193526CF02DF5B7" modified="2022-10-12" version="1">MGILHTSCVRECTNRDQNSNQELQVIQNKQIQKIQHHLTNEYETSKTTLKGTSQLSDTEKFMEIKEEDQSSNLKINPVPAFVLNKQLHQFIVPKRENRFIQDIEYEVKQKIQQIQNKEKFKALQLKYSKINDQQQPQKFDKCSSNVLNYINKQHVXSFQSFHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSL9</accession>
    <name>A0A8S1KSL9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270196</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSL9"/>
    <dbReference type="OMA" id="WVANYEL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="238" mass="28813" checksum="63F34A32FA7B6B9B" modified="2022-10-12" version="1">MNLNPETTLNLRIDENTKLVIKQIIVYDQFSETFNSLIKTYSTADHICAYTASANIRILQQYGIQEGLIKLKDMNFVKNFVEDMMKYVFQSRLSYAKQKWNNNIQKIKEYCRDWVANYELSDYMKTLKLENVYIFRHVGLFHKDLFEKTENQERERIIQDETPFKDQPYFIYYPKEDKYIRKDQFTIQDNHLYIFDTMGHFICGWVKNKDKKNKQITILETIPHRDSKTNKNLQIFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZD4</accession>
    <name>A0A8S1KZD4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0300069</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PRA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZD4"/>
    <dbReference type="OMA" id="HASLWKS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317:SF0">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8060307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="157" mass="17457" checksum="8CB389830E32E411" modified="2022-10-12" version="1">MQTQPGILDLSAYNLPKSDSLSLRIQENFSLYGVRYTQLVMVAIAIGGLTNFAVLLILFLSYLGWAYIMKQSSILDYLPDLGLINTQDKKMILLLLFNILLILAISGPNILSYVGLGCLIVIVHASLWKSPSTLPGQDIQSEFDKVLMQNDVELINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0E1</accession>
    <name>A0A8S1L0E1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8060877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L0E1"/>
    <dbReference type="OMA" id="QLNDQYV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="125" mass="15156" checksum="D25079407F949795" modified="2022-10-12" version="1">MSNSQSVQNSFEEFEEVDYFDIENYQYISQLIKMEEDFEKEQLFINRLELKLKLMIPFLLEQLNKDNLRQCISNLSKIIKNKQLNDQYVKVISNIKTQYHRLTLKQCKQEIAQILLWILFEIENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2P5</accession>
    <name>A0A8S1L2P5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0250113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2P5"/>
    <dbReference type="OMA" id="NESQQIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="126"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="181" mass="21216" checksum="4C26B33AE20B1FEC" modified="2022-10-12" version="1">MRQQQAHIQTLQTMAARLSSTISNRRSVLNESQQIYNQQLNLSVNQSFNSPKLISNHQSFIISQPMNQSMRETVQGVRCASPSLRIQTEKIEKEDKKQQQFIDATMKLLAQNEKLEKLICEQNEMLAIYKQDKQELEFKIKRLSQEIIFLKDANSKRVMALERELKQVKGKSQHQPKKFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2Q3</accession>
    <name>A0A8S1L2Q3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RCK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0290129</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2Q3"/>
    <dbReference type="OMA" id="GYKYIYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000146">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000797">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF10">
        <property type="entry name" value="SLOWPOKE 2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="14">
        <location>
            <begin position="626"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8059712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="990" mass="113605" checksum="AFB142295DD4CD91" modified="2022-10-12" version="1">MIYEGKQSIFSHQDQYALIRMVNSEDDATTKKHEEGKTTFTLKMAKYLSTSGIGDYLDGINVLLSFLLTVLHLIDCSFWVQGGNQDVGEGQAIITIPELICYIYFLLDFVINFYLSENKLFFTFQTTSLIEYVSIFPSLLARLNIITGYKYIYMLRVLRFLLCYKLDKVLQRLSMEAYKPIVTVMAIIMINSSVLYVVEQDYSIVEYIYFMVVTISTVGFGDVYPTTIYGRFSIIVAILIMFLVLPTQVEMLTRVYSLRSQYARNKYISKKESEHVLLLGSSQVEGFKTFLNELYHSDHGMNDTNTVILQPCAPTEEMTLQLKQPALQSKVIYLEGHPLQNKDLERCSSKDCNCVIVLANKTSRTPKRDDYRNIIHAFAVKQFAKKQKSRKGARVCLQVLQPSSKDLYFNSLGGHETDQVICVDELKLYLLGKTCLCPGINTLISFLIQSSKPSYDITKYDKNKGEWIDDYLCGMQNEIYRVPLESVAFVGLTFSQISSAIYKELNIILFALEVELESGTSVFVNPVEYLFEDYLHYGYVIANEMPNIDEIQQTKFPEYIQKNYCFPNQQRHQINKNALQQEAEYLKEILSEGIKDDSSKHPAYYQVKPQTITTGLPKVGDQEKFENHFIVCGVVTDMKYLMMPLRARSLKNIQPIVILNQDLIPTEIQLQINKFPKVYFQQGSPLNTEDLKKACIQKASALVILQKSADQEEGLSNIVDADTIFIYKTVKLLNQNINIITELASISTISFLQISRNNYVQKYDWSVSEPFASGEIYISTMLDTLICQAYYNPFITSIFDQMILGSASVNKKHKKLYQATKLQQSNLFLINIPPKYQEKTFGELFEILLTEQKMIPIGLYRGEKVKNNNKPYVFLKPPMDVVVSSKDRLYVLSGKQPKEQENILDNEKNQGKETASQYYNIKSKLQGEEGRVDIEFSRELMKFKDKMQSFVKGLNKINSEVFNQGLIHEEFISKVRGTLKEELANLNPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4W5</accession>
    <name>A0A8S1L4W5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UDP-N-acetylglucosamine pyrophosphorylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0290166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4W5"/>
    <dbReference type="OMA" id="KCVVQIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039741">
        <property type="entry name" value="UDP-sugar_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952:SF2">
        <property type="entry name" value="LD24639P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952">
        <property type="entry name" value="UDP- GLUCOSE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="52997" checksum="0580A177A007FDC3" modified="2022-10-12" version="1">MGICTSQNEAQKQENSEEDYQKLDFNRVSTYQKLENIANNIATQQQIDTIFSSLIQRMIASRQTHILNMLEKMDNKKKQQFMTKLERIDFEFIDSLFHHCYKQNQSDVFSCNVIQSINSIEQNYDLIKKHKVGIVLLCGGRSSRLSDKLLSDIGLPSKKCVVQIMMERLKKVIQIASENKEIVDYPIAILLSDQNSDKFQQYLKSKRDFGFQSITFLLEKSQPVVDIKGQVVFEYENQVIMTPEGTGTIFIQLNSFINKFPNMEYIHFFGLDNLVGLPLDPIMLNLICKQKADALCKVIETNQILDDRLFYQNKQFKTIKEQDQIIKENQIQMCLNDLYLSISFLNKLKQNHEKALKLNQRYHSIKRGDNIQFEKHIQDIIEVTDTTILYQTKDYALLIDDPKKAVIQLSNVHKRYLNLEGTQDKDLVEITPQMSYCKIEDLKKIENITYPLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5S7</accession>
    <name>A0A8S1L5S7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0260328</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8058004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5S7"/>
    <dbReference type="OMA" id="QECHQVI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="345" mass="40559" checksum="F67423EFB4887A64" modified="2022-10-12" version="1">MNLGIQQCQKIDHLKNPIIGICTDRVCQSQRLYCFLCIENHRDHLNSLIQYDWLRNQMVSSIQIIKEISFNIQECHQVIHILNSFIKSAEDCILNNLQEFQIENLDQQISQFRKLGQAQETLLPQLSELIKLLRLMGKEWENLEKRHSIENDFGVQNKIQQTKLIQKQYCDQKESIQSLINKHNQLLVRFEENYQCRYVDIQQYGKIVQGQGLFFGNQMIPKIGITKFTLKIWDVKAFVNIGVGDKDTIVRTNYAPNLKFIGHGAYLISNQGYNYTHLSKTQNDVQQSFIFNKYDVILIEINMNLKAIFWTKKSTLQQFQLEINNEQDLYPLIILHGKVEVLDNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L876</accession>
    <name>A0A8S1L876_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6815 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0350093</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0350094</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0350095</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L876"/>
    <dbReference type="OMA" id="VAWTHEL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049212">
        <property type="entry name" value="DUF6815"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033816">
        <property type="entry name" value="Cj0069_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20668">
        <property type="entry name" value="DUF6815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6815" evidence="1">
        <location>
            <begin position="230"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8063697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8063699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="357" mass="40872" checksum="E802019562D1520C" modified="2022-10-12" version="1">MAQVRKLHRRVAIFEAEGGSDKTWNGHRKDTMPIVEAFKELGWTAEVIFFRDEWKEAITKYVIENCDAYIPRINTGNLPNGEAVFNQALREMCAAGVVGTPHPDTLMKYDSKLSLVDLNKTPLSPADTVAYFKWDELVKNFPTSLTNGERVLKQNRGSTGEGIWRIQVAEGVQVVKGQALPLDTKIKCTEAVDNHVEHHTLESFFKLCEKYYRVEENFLIDMRFLPRIKEGEVRIFLMGTKPLFVIHKKPADKQDAFSATLFSGATYKYESPEAWPELIKFFTSCLQYLTDNLGDVETILDWTCDFILDTDENGKDKYWISEVNVSCIGFTNQLDIGIQQEMAQELIRKVYKKKGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LG65</accession>
    <name>A0A8S1LG65_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370278</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LG65"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244:SF34">
        <property type="entry name" value="DTDP-4-AMINO-4,6-DIDEOXYGALACTOSE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="193" mass="22586" checksum="72355884FD1747D6" modified="2022-10-12" version="1">MVQVLRLNGLIPLPIEVDPHTLGCTLDQFKKFYSQRTKGIMISYVFGTKFDSSDIIDWAQSKGLFIMEDEAESFNAPNAKLNPNVEFSTFSFSSIKTYSAFGGSISVIRNNEVLYSKMKAIQETQPKWSQRNQDINQNFQLFEENFKKFNPNDFVKQQNNKKKFKMDFQQFLFYQALQRICSQYYSRIPKKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHH4</accession>
    <name>A0A8S1LHH4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0410058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHH4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="106" mass="12557" checksum="0AE1E8558894A1C0" modified="2022-10-12" version="1">MDLYQHSYTLPPKRLCKEPKLAISILKRKFIEPIQDFTGARRTRTSFSPQRRMKSIQIRQKSAIQTFNFEVGQIIVPKRNSRVTRFQGPNFPFNLNYLIKQMNALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKB6</accession>
    <name>A0A8S1LKB6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0400007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8066895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LKB6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="28995" checksum="91D505E4ED9D9E83" modified="2022-10-12" version="1">MLKILLQIEREIKNFNYLTQCAFALFRLLYSYLSVLLLQNLLEIYLQMMHQNLFIYHFDKQNIKILFFFNFYEGAFQSSPSYLQVYLSNFQTELHNLVANTLSQTGSIRTHEQRFQLLIVNIHLTQDSRLKWTTALQIFQCFASIIGRGINYRMRNNQNKYNNIKKVEIQSTQIEQLRSRKNIDLEQSVIKFLLILRTFSYQTYQIFLKDTYGQFVLSPHDQYQRNKNVSQYLKLNSIEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQN6</accession>
    <name>A0A8S1LQN6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Ribonuclease P/MRP protein subunit POP5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0450052</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQN6"/>
    <dbReference type="OMA" id="LKCDMPN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016819">
        <property type="entry name" value="RNase_P/MRP_POP5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48414">
        <property type="entry name" value="POP5 HOMOLOG, RIBONUCLEASE P_MRP SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48414:SF1">
        <property type="entry name" value="POP5 HOMOLOG, RIBONUCLEASE P_MRP SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023803">
        <property type="entry name" value="Ribonuclease_P_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR023803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8070087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="148" mass="17877" checksum="32AD1A14C277B692" modified="2022-10-12" version="1">MVRFKNRYFLCEYIQENQEKEFSERDLLNEIRDQVQYHFGDFGSAKIQFSFQVKYLNSVSRLFILRVNRDYKSIIWSTLIFMNMFKGVPIKIRILGCSGTIKKCEIKARRLLTKWVHKILKCDMPNQLRNTIIRDYQQTQQILPQITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUE1</accession>
    <name>A0A8S1LUE1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0430274</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUE1"/>
    <dbReference type="OMA" id="CEYIKQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="2">
        <location>
            <begin position="558"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8069253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="891" mass="105600" checksum="02FF5C8F4D736561" modified="2022-10-12" version="1">MSYLAQSSRLTALLISLKNKKLITPDQYTRLCDYAINDNIELKSLFVDYEKGLSQNDFLSGLSIILNNKTESEHIIKSYFESVVSINQQLKEKIKEFYRDIKSNMNEIYEECFKAQHHLQEVKDLIKLVLKQFYVEEFYLIQFEEGHQCVILTNNFDFRFFKEEYKISSWAHKQNAHQLNNTQTEHYALLKTHNLPKQYIISQHAVLFLTFSDSVTKTYSQYFMATFLYDKFITLIYQGYQIVGLKKMQSIRTEILQLVSKTILLYNYKLMYQILIQIMQQFKLQNLLCQFEKELTKQQTVTNYLLILLSSDLDIIGIELEGNLSFQLVDKVKSTFKKSIKKYKKKLKSKKDLLESFQQVTQHNEMIMCLFFNNYFNLYYMTVQWNHDQFVSKYNLPKEIYYGDPIYKIFDQNPTIIQKVKEMIDSPNDHLEYNDGFFKFSLKIERGFKQQIKQISIFLFNIQVKRPLAELFRIFRSKVKILLTIRKATLAFKSKQLMMENQFLRQSALVMYLPDQDRRRITQTHNQDHDQDLEKYRNYTMKQATKKLQQFLNKCDEEDTKNPELRRIAIDPNLQQKLLDYEFNLLNKKMYKNKYIIAYNLFHMCEYIKQYPLLTKEFINFMSVLKYKYNKKSNPFHNFTHGVNVMHGCYLFGHHQKFGSYFSDLQRFAMTFAGLCHDVDHSGTTNLFQVNAQTKLALLYNDKSVLENHHVAVTYKILAHKQCDFFGSIPKQDKITIRKYVVNNILATDNQYHFNLLNDIEIRFGQSKGDPKIFETEENKLLLSGFLTHAADFFGAAKQQPIARAWSERLRKEFQAQSQLEDIIGIAQTPYLRNLDNEVQYAKNEAGFLKVIVKPIYESLNNYSDGALALQLANINLSIKKYEEIIEQANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUN1</accession>
    <name>A0A8S1LUN1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0430073</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0460220</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May play a role in vesicular transport from endoplasmic reticulum to Golgi.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2">Golgi apparatus</location>
            <location evidence="1 2">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8068901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUN1"/>
    <dbReference type="OMA" id="MVQMQVQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14942">
        <property type="entry name" value="TRAPPC3_bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016721">
        <property type="entry name" value="Bet3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13048">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018293">
        <property type="entry name" value="TRAPP_I_complex_Bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR018293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8068901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8070949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="181" mass="20719" checksum="99EA00A079F53695" modified="2022-10-12" version="1">MQIRKSNKVVVNRIEKVNSELLTLTYGAIVSQIIKDSDTVIEANEQLEKMGYNIGQRIIDEYLSKAEIKQCRDKIDVAENIGKGAMQMFLGVTAEVEILPETDKIFTFNLHFIENPLNDFVELPEHLQGLNYSNMIAGAIRGALSTIHWIATCRFLKDQLKNDDKTILQVEMIREKFKDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LWW9</accession>
    <name>A0A8S1LWW9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0450035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LWW9"/>
    <dbReference type="OMA" id="QREAMDF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="184" mass="21759" checksum="B3AC0C4E4F0D2B49" modified="2022-10-12" version="1">MQFENNRIGTIEEIEYLQNDIFPTLHTALAKLVEYVEKTEEVNKHKERLEQIKICDKIEKRRVERDRLKKELGSDFGSDSEGQEENPENKSINDEQTGKKLEQLIPIENEFQQQSQNLIDEITMQEMKQEINHLLGQIQEDQGEEESPDKHELEKLKLQLRIQREAMDFNPLIYLASLLRQLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LY30</accession>
    <name>A0A8S1LY30_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LY30"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="123" mass="14662" checksum="E03E7B646F8C3840" modified="2022-10-12" version="1">MKEIQLIIKEVIIQKIKSIQSKQYSLLYKKLEESLKEVREESIFQGSCTFEQDYVLFSSSQSKSQRFLSFLLMCGKRISIYLMKQLNNTEYDFFIYGSFMFLKREIGGEKPPFLQNTNRITHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZQ4</accession>
    <name>A0A8S1LZQ4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0510121</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZQ4"/>
    <dbReference type="OMA" id="CKMEIFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000082">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051630">
        <property type="entry name" value="Corepressor-Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017:SF28">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_98, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="4">
        <location>
            <begin position="194"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="512"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="522"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="553"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="606"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8073550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="633" mass="74447" checksum="E60F303E383B1B65" modified="2022-10-12" version="1">MYLSFIKEHFTPSQEELDILEAKETQDLYSDVQIQGGFIQVYSRQCSIFDSELYKKQLKVFQKYLLNYEKQNPKLNGLCLEKAFYVEQTYSLDCQVESPETWLREQVTVWQLPTDIVNKLNSKCFTFEDIAKRHGNQMVDIRIQDPNTDTFTPTIEQNCIANQMRIDEYCKYLKNKTEFFQKHTEYSSDKVLFAVNVDMDNWDEETGQLYEYMPQSLLKYDGLVYMRQFCLGVNIPQIYIKTKGVWTGGHQENSSVNSLNFNHGPGDCLWLTVDAQHVKKLYEIMPNLYQIEGLWFKEIDFFLENNIPIKYTIQKKGDLVILGAGCLHWVKSLGNTINTSWNLLVYQEDTFQQIYDRQLINDQYQISSVLPIKNLFLDIYIHRKSPLLKTFLHKFIQEDIDNYLKQSTKIKNCKKKFNSREVLQCNNCKMEIFIFYQIKNDQTFRCIKCLDGDEIEIFMKYKPTELILVLNAEKFSCSPTLCTRYMGNTECQLDKQTEEFLSESYQSSIDLSKSAEEQSKTKFTRRKEKSKTSKKSSQTQQQDRNSSAQTNHQIRKKRPYRKKTKGQDDLISEICTSQKSHKKNENNNTKSKLNKKIQKIQKLENSVKSVSSSGFDSHSQIQLIKKGKKQNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M1V1</accession>
    <name>A0A8S1M1V1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0490037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M1V1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="98" mass="12061" checksum="6883E61103BDAFB6" modified="2022-10-12" version="1">MEIIGECQNYGKMRNQKKLVYIVKKMIVIAKSIMIIKFNRSFQDQLDIHQIKWMRKNKHLIQYMILLMKEDIQHMKALLQKQLQNFIYQQKEIFLQVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3R7</accession>
    <name>A0A8S1M3R7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0530075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3R7"/>
    <dbReference type="OMA" id="CGFINHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="40001" checksum="B7C2E92D367D78AF" modified="2022-10-12" version="1">MTECKDKDLDEIYKEHCNENFNSTDKIVTFILRLIILMISIYSSFQVLLIIKKSNKKQFWPFQLISFQVISESIDLVFALLFTINSSCWPNACKFVGYIMHSNWLASLLYMLFQCFIYFLLIKSEILFNFVMKYLYFFLFTFYFIPYGFLYKVFSDDGFGPSGWYLKNGEFNFIFCGFINHQIIGFWIVPVGIIFSLSIILSIYNSCYWYHLQYKTELRYPSLRIQSMIVFPLLYLFAWIINFLIRLKDTAANNQNYCPQQNKNLVFYIFYQFLNLGFELHLTLGAILFFYIYRGLFRIDSLCDQICLRKRRYKKQNKKKLYQDQSVLAIQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5B3</accession>
    <name>A0A8S1M5B3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0500130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5B3"/>
    <dbReference type="OMA" id="FEGQKQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8072951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="623" mass="72238" checksum="605EA06CD816BCF1" modified="2022-10-12" version="1">MKSTNMKKLGNLSKKLDFFSSPVELMIHKKRAHQSLFGAFMTLLMFSCVLTYIINKFVQLGQRKEFQTLYSEVYYEEIPVFPLQSKNFSLQFAFQTDNQTNYIDKSIYQVKAVMVNRAQVMVDNKTNLQLTKTEIPLQKCGKIGIPYEEISDQLDNVDIENTYCLDWNNIKDLSLIGTPEQENYTYIYISFQSCVNDTSNINSQVCKSKEEIQEKLHRNYILFQLSSYNIDLRNYLKPNVPKVEEIQTTISSQVMKDITLFMQPITTLTEEGLLNGLVRKDSTIRYQKTQEVIDFNSDEALASVLIRLANTENISYRIYPKLQDILAQAGGLWEILMLVFTILVKPFQSLSYKLAVINNLFNFEGQKQQKDIEDGGKQILKKLTMVQENVQTNENDASIMLNQSKIKTSIRFPRGRTKSKHQKLEQINSTQTEKSSQIVTNSPENDKLIQKGIRFALRKLFNIATLKLQLSPIDYIKYLKCGKKEGKFKQMQYSVNKLEKCLDILFIIDKLQEIDKLKTILLTKQQVQLFDFLPKPLISLDPSNLQQNETIMYSSLLKPYKTQREKAHEAQQVLDDLLENLDDPITIKLISLMDPNIFKLLQIQQDLKKRQVSKLITPDIIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M7R8</accession>
    <name>A0A8S1M7R8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinase domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0530191</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M7R8"/>
    <dbReference type="OMA" id="SHTIQHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="665" mass="76055" checksum="9217ACF2BBA57FF5" modified="2022-10-12" version="1">MTLSHTIQHQEDIQCLKSETLQIVIQTSEQVVLDKIECKNLTLVVEGFLNYQHYSTLISSLNHLDLSSFKLKYGANNQFSDEDGKILAQFILNQKSLKSLDIHFDLNNPIKQISPVLEAIQNHDNELVHLKLNFEDELPLCLKDLQFITSKKLQSISLQIGQGVCVDQNWSHFEDAQKDSILQEFTLLISPFNKLDKSFHSFFKWLASQPNIEKLNISIGAQTEIDEQAITQLGLALQKIEQLKFLNLFLHDTRNLTQNSFDQIVKSFGKLENLELNLGNNYGINNYDILYQQVKNIKQVKLSLGTHHKFISSELFQDCNIKILDIKLQKGSTIKQGSFKSIEPFLERTEKLLIELSEGKDCSDLDLIDLIGQITPGCDIRITTNTQQIRKIKQTLQIQLDTIQSIKILFNTLSQKMDLFQNLKIFIKSTSIEQESVDSIIKQLQQSQLKVLSLIIKKINNFQCNQMIDLFQQIVVLKQWTFLSIEVVQKCIMQLYGKSLTNQQVNFQVHSGLKLGSQGMEYIAKSLKSINITKLRLTILLDNSLNEQGLKQITLALLNSVNLQKASLQILDSNQQLQQYMSKMQATINQYIKDKTKLQKSYCLFLQKELNNNKKQISQQMLAGVADYLIEDVVLPVPAAGQYHNQQGGFGSSWNPQQIVEIFGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8C3</accession>
    <name>A0A8S1M8C3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0520079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8C3"/>
    <dbReference type="OMA" id="NIKIWIE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="271"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="321"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="365"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="408"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="198"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="542" mass="63042" checksum="CF643AC1A6BABB58" modified="2022-10-12" version="1">MIKLEEKASKILCSQHQKSIQYITLSKTSNNRLLCQKCPQKNRIAINKASELLMDINQDHPLNYHIKELQKQYESLRKEIKLKVDELLDETEDCLNSLINHKLSDKQIQHYLYDDKLNEQDFEKLQEILCKYYDFEQDNQIVAKIPSNDMHNVYQVQQKCQKIASIIKAFYKSQYHSRLGTQQSQENLLSQIPLKKNTKAEKPSEQDKHEEEKSKGLYTIKEEIITNSVCHCIDLNKTATLIVCGLQGNNNEDNLVLLQKKEEGFKEFQTLVGHVGSINAVCFGKINNSIFSAGDDKTIRIWNEYLQSKENKLYNCKQIINEAHNMEIHCLCLNQLNNLLASGCQEGNIKIWIEVDEKWINSQVLIQHSQWVKSLSFNYDSTFLVSGSGDKLICVWNLNEDWEHFQTITEHTNLVNCVNFAQGENIFVSGSEDQTIKIWNQQNEPNGKFICIQQLEGGYYIRSVCFNYNSLLLVSGSYSNIKVWVKNHAGVFVLNQQKYFDSRQVLLSEDSKDLFSVNRFSLNIRMFRIKEKNKKNNTKLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9Z7</accession>
    <name>A0A8S1M9Z7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Isopentenyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0570194</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8077060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF60">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8077060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="32565" checksum="B460E722E9238AA4" modified="2022-10-12" version="1" fragment="single">MNNNGFIVYIIGTTAAGKTNLSLNLGLDNYEIISCDSMQIYKDANIMTAKVTAIEQTIKMHHGIDLLELESEGFNRKQWKHMTINKIEEIQSKGKIPVLVGNTHNFIESILFNQETEENQIQQAAEFHLDGKEPFEYLKEIDPLAALKFHPNDHRRIQNSIKYYQNIGQLQFDQLDHNSEQFKLRSNNFLIIWPKWRKNEDLKIKKKEEQKKYQEYLKDQRIKIISQVVYQKATDIVNSKKFISIINNQDFNILILMKIFKCYQNKVLKNQLMIRX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBG7</accession>
    <name>A0A8S1MBG7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0540026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBG7"/>
    <dbReference type="OMA" id="NSTIICA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="674" mass="79374" checksum="507C608C42C5CC2F" modified="2022-10-12" version="1">MNQLTQGISKFDIFGQTITLKMNKQNYYKTFFGGIVSIIVFLIMIMFFSSGIKSFFNKETLVATVLTDFEEIPSQSIIDDATFLFAIQIDQVNFLSRPFFDIEVLQVLETRQFNGSKIREEQQIQLVPCTLERFTTIFEKYNKNISQTYTDLNLDKFLCFKKNATFILEGTHSSIQFSYLKISIKKCNSTIICASQEERDAEIKLNGSFKIKLYTVNKILNPYNPSENYLQTYIDDSFYINFLPSIISKSANLFLKQFQVESDQSLTPFQQITKDQFYILDQSEVRERVDFYKEDNVEVATIFLRKSPYKTQIYRSYLKIDDLLSNLGGIQQILFFFLGFILALYNRFQLLIELANKLYEFMLVEKQSKRMHENNLNLVNQLITERQYLDSKVTECDATIQLLNQTQVKQKENNSKLKFEDQQQAIFKVNRKGKTFLKKSTKFYYDEQKKAYRSHISNGLEYFESQINLLIRRAKPIKLSFQLLMNYISCQLLFRKKRKIVLMNKAMNQLSNQIDLFNILTKLNEIDKLKEVLLTSEQQLLFDFTPKPIISLDYKDVKIDRFLIEQNSRCDLDLEIQQKEKQIKEVEISQKSLHLKADSSLYERIYKAYDKVLNGLTVSNQYEQYINNQLINQLGAEVQTIFKLSKLIDFSVSFSRTFRRKGQIFQKEIDPISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCP9</accession>
    <name>A0A8S1MCP9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0550169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCP9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="118" mass="13606" checksum="E076E675F2798A69" modified="2022-10-12" version="1">MNHYENQANKHTLKVPNGNKTVYDPKGPAIFLLLVELKQYQEKAQMLKDDQGISGILDQLCKGFIKLNLDETLRSFQQIIFQSAKQQSLEHSLIQPYPSQQQFAKQQKFVLMIGQTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDW0</accession>
    <name>A0A8S1MDW0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0550133</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDW0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040132">
        <property type="entry name" value="Tex1/THOC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3 THO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="195"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8075903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="323" mass="37028" checksum="6E71E1CEA9968F50" modified="2022-10-12" version="1">MFYQQFKEYPKNSFREKTQQERKIIKSMDWNCNGSYLATTSDSTIKIWSFDGNNIKRSTDLKQGSEQLEKLLFHPTQPDIIASITKEYVKIWDIRTKQSVRQEKKEQIGIACWSPNGNELAVGTKQNKETQIMFLDMQGGERQILYQPKDKDKNFELKNIIYDKDVFVAVGQQNGVGMIQFMDVNNPQLPPLHQMEAHRAGITQCKFSKDGQHMYTGAQDSLIILWQLPDLLCAGQLMTVESEIKSISIFDSKYIAAIGLEEQAYIYQVDRCLAGKTDHIDSIKLEGQPSDICFHPNKFILAIVCQDKNKQESVIHLYGNLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEI7</accession>
    <name>A0A8S1MEI7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0570058</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEI7"/>
    <dbReference type="OMA" id="KSEFEPR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:001518">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="3">
        <location>
            <begin position="381"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8076791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="733" mass="88080" checksum="2E38BACC3254B32E" modified="2022-10-12" version="1">MKKIIQENLSPDEAKTLWKYLDDACYLIAEHQTKQLSFQELYQHGYKLTLNKFGDFAYSQLQISIHNIVDKLIYSLNISNDDQLLNDFVKAYEEVQQFSKLLAGILLYMEKTYILQKSLKTIKTICNESFKIKCFEKDQNLATKLLNCFLNQIRNDRNSQVIEHFKLKNALQILIDINQEQQIKNKEYNYIIDYRINDDFYKYFLERKLIQDSNRYFKEESQLNLNRMTIEEYILFVEKRYQNEVERVESYIPKISHQKVLDCFIKNYITYNAQYISQGLYDFIENEKTTIIIKIFQLFVKSEEFDLFIKTLSQIIIDDLQLLNQNDNTLQGFIKLYEKIFSLYDKIVCYGNNEHQYKFHKAIKNAFEQVINKDNFIMMELNKYFDFLMKKETLRDEEKKIQIDKGFLIFKLVQSKDEFEQIYRKYLCVRLLDQTSSSLEVEKDLLKKLRLECGSVLTHKMETMFNDLQRSNEDSQKFRQKLSSNLRDTIDLDILVLTSEQWPIEESQPIVVHTELFQWQQQFTQYYQSKNQKRKLTFNYGLGSVSLKATFDSNCKKDFVCSVLQATILMHFNKQRVYKVDQLIKLTNTDQEIMQSELENLILFKILIQNEEENSLQLNYKFQHRSYKIKVQPKKTQSWAICSKSKTKQSEFQINQKEIVLDRRVYLESLIVRVLKTKKQLDHKDLFKYIDKDAKSRHFPVEIPFFKECIENLIQKEYLIRQEGQLDTYIYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEY7</accession>
    <name>A0A8S1MEY7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Isopropylmalate dehydrogenase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0610168</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEY7"/>
    <dbReference type="OMA" id="TCAHKAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF42">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="3">
        <location>
            <begin position="34"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8079190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="355" mass="39662" checksum="DC7E1CC85D637BB2" modified="2022-10-12" version="1">MKNCVRQAFRSNSTLRGAWSQNYLQRPGEGQLKNVTVLPGDGIGPEITRSVMQVFDALHVPVKFDVLDNFNFDNDEQRNLLKKNECILLGVMTEKNQKYTDNYRFYKYLDLYANITFAFSVEGIKQRHNNTDIVVIRENTEGEYSGVEHEVYPGVVESIKVTTKQASLRIAEYAFEFAHLSGRKKVTAVHKANIMKLVDGLFLSAHREVAQKYPFIKYEEMIIDNCCMQLVKNPTQFDVMVMPNLYGSIVQNVVAGITGGVGMAAGASIGKDHALFSQGCRHTGRDIAGKNIVNPSALLVSSSLLLRHLGLPNFADQICSAVQETIQERKLKTKDIGGKASTEQFTKEVIKCLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MGM7</accession>
    <name>A0A8S1MGM7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0570079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MGM7"/>
    <dbReference type="OMA" id="SCPVRML"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8076833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="110" mass="12963" checksum="30EC5B741CB36B3D" modified="2022-10-12" version="1">MGGLAQKVKNLFVKQEELLPIQDDNNYHSQIQNQQYEFDKNSSGNFNQMESQNQNLNDGKLYSMSSMLKVLDKYRSCPVRMLTMMQGKQYDTQLYQNEQKKIEEIITKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJI3</accession>
    <name>A0A8S1MJI3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CoA transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0600167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJI3"/>
    <dbReference type="OMA" id="IIAGPYC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050483">
        <property type="entry name" value="CoA-transferase_III_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48207">
        <property type="entry name" value="SUCCINATE--HYDROXYMETHYLGLUTARATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48207:SF3">
        <property type="entry name" value="SUCCINATE--HYDROXYMETHYLGLUTARATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8078621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="43156" checksum="AF258A61EAE7ABD7" modified="2022-10-12" version="1">MYKQLKVLDLSRILVGPYATMLLSDLGAQVIKVESFEGDETRKWGPPFFNNQSTYFLSINRSKQSICVDLKKGNQFIKQLVEQSDVLVENFTSGVMDRLGLGYDELSKINPRLIYASVNGFGSNNNQPGFDYIMQAETGFMHITGEKDREPMKVGVAIIDVLTALNLCNGIQGALYQRIHTNKGCHIQTSLYESAIASLVNVSGMYLNGQKDMHRMGNQHPSISPYGTFKIKNKYIVIGVAQDNQFQSLCKILNLNHLINDEKYKENKGRVQNSEKLKRIIEEALENWDVENLINEMKKNKIPVGEVKSVGQCLDSQHSKSLNMVIELENGEKIIRNPLCFSNINLDYPKQAPYLNEHRDEICKQFGINNVDKLIQDGILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MP76</accession>
    <name>A0A8S1MP76_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0600130</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MP76"/>
    <dbReference type="OMA" id="FTSFQQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF36">
        <property type="entry name" value="KINESIN HEAVY CHAIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="80"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="603"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="147"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8078565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="630" mass="72858" checksum="4C81095E73210458" modified="2022-10-12" version="1">MSYNSKTIDLILKVQEPNQKQFTSFQQFFKTNNPPPLLFEKNITPKNVKAQRSFSNKTQDSIKETKRKSFSQPKYPEYRMLKIYARIKPGECQLNYDKSSILIDTNSYNFDRVFEQQATQGDIFDVVAKQTVDDFLNNINGCLIAYGQTGSGKTFTMFGESNNPGIVLRTFTHLLQRNQETIYVSILEIYKDHIYDLVSGVQDLKMKEDLQLGFYVDGLKRIKVDKLENCQELLQIAEENRHVAETKLNALSSRSHLILTIQMGRAKLYLVDLAGSEKVNKTGAIGETLQEAKKINYSLSCLGHVIQCLSQGQDHIPYRDSKLTKLLMDSLQADCRTSIIVAISPENKNLDETVSSLKFAQRARFIKKEIRIATQKKTYRDLELEISQLKQELLETKQRMSQTSSVSQFQCRYTTDSEIPCSESLRLTYRSLNTENKENCVPNLKIQENSAQLELDKKINQILIELQGLNKEQNFDNVKRQVVKINELTIEMKKTYKLLVSTQGLNNNHLQIQCQFIETQQEQNKQLQKIAEQISNMDSSKPSFNIAINNMIKNLQKQQQQNIDDMQCKLEKMYYDIKKTATAQDYNFNHLALKLVASPNRQQQYQQQQMKRSKSKPNINPSVKYLKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQ04</accession>
    <name>A0A8S1MQ04_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0690129</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQ04"/>
    <dbReference type="OMA" id="MTSFNLY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000089">
        <property type="entry name" value="Cyclin, N-terminal domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="146"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8083137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="365" mass="43429" checksum="73B251564B07A13F" modified="2022-10-12" version="1">MKQDRNFSMSGRSTSPQLKTQKTQKLNKNLYSQQSSSCKTNSKSQEKQPLQPKEKERKILNFIKELEVQQQKEIINQKLIHLYLKDAQILLPINEDQDYSELRRQLHYHQNYHQNFSSSMISEYVYLGYSLSNQQISYKLRAKMINWMIEVLGNYSETTSDATFFRSISIMDYYLKKTIFQYTDNQLHLIGITSMFIATKIEDIYQIPLQDFVTRICHNEYSISKIKEMEQSILETLNFEITFPTALDFLKNIFYQCFSLNDNPYLQNILDTSIYILKMCLHDYSMTSFNLYTLASSSLIYSITDFVNKNYLNNQQIIINQFQNKIVQIFQIDQIELNLCEKNLQDLIDTFQFKYPKLHNLRIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTF4</accession>
    <name>A0A8S1MTF4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0660011</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTF4"/>
    <dbReference type="OMA" id="PNSHDSY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008318">
        <property type="term" value="F:protein prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="40"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="142"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="176"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="210"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="244"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="278"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="312"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="346"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="380"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="414"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="448"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="482"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="516"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8081471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="592" mass="68442" checksum="94A5B907F6A8CE2C" modified="2022-10-12" version="1">MIPQEIQVDDKNFSEYQDVITMVEIKQGSDDVKQQNIQEAYRILNKGLALHNLQKYQEAIECYDQAILINPTYYTAWNNKGFALHHLQKYQEAIECYDKAILINPKSDAGWNNKGFSLNNLKKYVKAIECCDNAIQINSQNNSAWSNKGFALDKLKKYQEAIECYDKVIQINPKCDVALSDKGLALYNLQKYQEAIEQCEKAIQINPQNNSAWNHKGLALDKLQKYQEAIECYDQAIQINSKYDIAWTNKGLALNTLKKYKEAFGCYDKAIALNPKNDTTWINAGQALYNLQKYQEAIGCYDKAILIDPINYPALNNKGFALDKLQKYQEAIECYDKAISINPKYDVAWNNKSLALINLQKYQEAIECCDQAILINPQNNSAWNHKGLALGKLQQYQLAFECFDKVILINPKNDFAWNNKGLILHNLQKYQKAIECYEKVIQINPKFHSVWNNKGLSLFELQKYKQAIECYEKAISFYPKHDSTWVNKGKALHKLQKYKEAIECYEKAIQINPKQDKAWINKGVALHNQKQYKEAILCYDEALSINFNPINLRFKADSLLELGEKNEAKQFYMSALEKGLNQEYIEKQLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTP9</accession>
    <name>A0A8S1MTP9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0680147</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTP9"/>
    <dbReference type="OMA" id="KYIELQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="473"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="399"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="484"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8082762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="585" mass="68795" checksum="623D26ED4945BCFB" modified="2022-10-12" version="1">MDQVDMIQDDQQSPLHIKRQLDIDISNRVAYPINLDQQKKETPKTTERTIKHLEIDEVQVLTETTSKTQIDVSSPRVPFLTHSQIMGMHQEEDEVPFNRSALNYPLSEIPEELTPSIMPHISKVSNQEVIIDNMSIVNSQQLKFSKKSSETNVVSIINIDGSKLKEELKEHISLLESKVQELEKENIILKKKSNQEKIKYIELQMETEYKLTSIIEQFQDQEINYKKQIDILKNDLNNAKLQIHIINQQIPHNQVQQLSQSQIQLSYNNISQQQYNKQSTLSQSFNEQLNKNQKDLYLTQIQYEIVHSKLIKIIKKIDQQIDINKKDLFDLLQILENKVQQIVNSEEEEKIDEHRTLLSTLKIELGEIKQIRGQLSRVFYDHDQFIQKRKNESINDLKINELTLQIQEFKQQKLELIEQTQKQQDQIKQQNQIIIELQQKLNNSSQTDTQRMQSQFIRQLQTNPIKNIKESIKQKQEFSKKQNLKLQNSPMSRSENASPSLKFLNSPQNSNRFSTHQKQQKDDKQQEFCYQTSDFSVYYNSIHQSTTQNFNNNVNSNSEIIQKLIEQFKGNSIFAQKISGYSKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MVP5</accession>
    <name>A0A8S1MVP5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">STOP protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0610014</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM154 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8078884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MVP5"/>
    <dbReference type="OMA" id="SKNCKLN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033336">
        <property type="entry name" value="SAXO1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516:SF17">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8078884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="47629" checksum="DAE9A5B1E1B1796B" modified="2022-10-12" version="1">MSSQGQNTKLYTFQSNNSQSFINKPRMVKYQVLRKQTGLGSRIIRQSDSNVEEQIIETCKSLMPTRPDTQQSKQKSGTQTVQEKPQTREKVVSRLRTPKSESLLRIKSQENQIEDFQPINQQFNYVDDIPLDHPIWKLILPDVQNVNIPKLINDNPDYFHHQLGFCACYKCECGQCKCNFSKTCKLNYTSSPNTVYGKDFLHKQTGYNSLRPLNQTYYSTQFCNQKPIDQQSLHQTSYKQFKVPVQEMLRPQSQEHKGDFLGNSSYKTQFKFWGPPETAHFKRPVHQSVSDQIPFTAQSLYKDSFANKPYQKPSDSLKMITELTPLPTGQPFVSQSQTQLAYQPFRIQKNPKLNSDKIMDYGRSPKYDTQYKSLVNTEYTPKRQRYCPAKEYIAHYQQRTQQKLKQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWG9</accession>
    <name>A0A8S1MWG9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0710121</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWG9"/>
    <dbReference type="OMA" id="TIRIFFF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="442"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="736"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8083989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="859" mass="99902" checksum="35344F15D7B02F92" modified="2022-10-12" version="1">MMINQSSSRKLQISMIISLLFQFIRIIIQYQKLENQFVNFLMINLLAQIVLELLCFKKTCFFQTELKLALILMDIILFQVESFMYGVDNGYQNYFYIVIVHNLTNMKINIADSRIWKTIRIFFFIILLLSFSYQLNLNSSLSHEINYQYIVIVNQKLYLLLIMWLHSQKNTIQIVKPNQSSVNGFQDTPFETFQNTTLRQTQLKINPKRTIIRDLSFISTDKQQKNQINDTLSQIDYSSLFDVLSEGVIIVQFEKFDNITNSKPKIEYQNQNSKKIFQKNNHDLLILFEKLSCEILMDESPLHSRDSLFSLQSLNQQQIKYLKSSKLNFLKYYSQDQQITQRHTEQNQSPRFRSLTSQLNTVYKCLLYVFGTKRFRRETTENTNVLVIETSFEIENTKRSIEITISIGSEDLLFIIARDVIHRKNIKELLQINQSKSKTLSFVSHEFRSPLNVVINILSRLKEQLNNFDYICQNISIVLENSYYMLNLANDLLDLAQIKADQFTLIQKTFNLTELGEECLQMFKLQAELKNIKLNVISNVKCLQIKTDRNRLKQILVNLIANALKFTQKGAISIKFVQQGLLVNAGVEDTGVGISQNNLTKLFKAFGKIKEGLSENLNEQGVGLGLLISNKLAQQLSYDNTGLKVISQDTTQVKHGSYFYLTLKIQQLHHKCPCVKPQQIYLEDDYSPSLNEIRLNNQRSQIQQQYQIIINSENQSISESIEVKNEIQDNKITCKHILIVDDNVFNQQVLEMQIKQFSSSQIDKVFNGTDALDYIQQKKCSEFCQGYSAIFMDMEMPGLNGIQTSTQILKTNLQMKIFITSGYDEIKMKEQGNKIGIKEFLVKPISKQIIQRVIKQYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWT7</accession>
    <name>A0A8S1MWT7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0660001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWT7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001434">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="222"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="267"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="105"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8081455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="56887" checksum="71BA7C10F0A3E74F" modified="2022-10-12" version="1">MSSSQGSPFTSKFYRESIEDCFNKAQEVIESSYNKISQSIDQILLDSVLPRLPSLQFESANQQSSSLKSSTIPKEFIYPILINQITTELRQPINSIVQREVNKSEQKQTEVIQSLKQQVENQKEERKKEQRDYLEIINTKNSEIKQLNEKQIIEENYIQKIKQVQDSNKFTFELMEQNSIKVDNYCYAFAFNKDQSIVLAGCQKDIKVFENQQGKLNQVQLLSKHTNQVFTLNFMKKTNNFVSGSSDNSIIIWQVIANNQWNCQQILNGHSNWIFCLLLNNTDDLIISGSVDKTIKFWMKQNQWICQQTINDHAEQVYSLSLNEQQNKLISCSLDSQILIIEQSKLDKQWSVIQKIIVDQYGQRLCFINDNLFTFQPYCKDQMYIYEMDTNNKQYNKIKEIAVKCDSSYCDCLFPQQYRKSKCLLVNKNGKNVNLMRKKQNGDFIIQQSIQFGHQHIYGQLSDDGEYLITWDLGSKEIQIRKCKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N176</accession>
    <name>A0A8S1N176_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0690037</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8082955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N176"/>
    <dbReference type="OMA" id="SKQNERM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001817">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8082955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="424" mass="48453" checksum="76B73E5BD211E2F8" modified="2022-10-12" version="1">MRIEDMITTGTLRSYGDQSPVKKMLHAPTMKLYVVKEEPLHNKEIRKNLKDWISFWQNKFANSNLHVQVYATFWNTPEGYVSIVMEYMNGGSLQNLLESMGFLPERSIKQLVPPILQGLQKIHQQGAQCHGALGPSQILFLRDGTVKLGQGLQYRVQVQGNQLFNSYILGKPKDVQSLYDPTILETPSLWSKASSEKVHFPAQEDFILERANDIWKLGWLVLNCAIGTLEFHPKAQQIYEGSRIIIEEIAKYTDHLKDTCCLLHSEAKVISLVEQNQKTVSEPTKITILELLPSDKFSPEFIQFLCSTLKIDPRQRIYTEQLLQHPWLVSGRDPKGPNVQLKELLNISNAWNAFLPEEFQGQGAQKLERLCDALYLVLQNSERPTVNKMQLQENSPIIKELSYDMGLNAKAVSDRIMSIFQSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1W8</accession>
    <name>A0A8S1N1W8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0700207</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1W8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="69" mass="8144" checksum="B0651B106260F7BF" modified="2022-10-12" version="1">MFMWLKYFESEDGVCISCLNQLGKSQVSCKYQDCKDQIWTYGEESDDGNDVNRDGYSNYKIDLQLHLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N285</accession>
    <name>A0A8S1N285_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0770224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8087038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N285"/>
    <dbReference type="OMA" id="ICLMNAF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="35216" checksum="B09774D8D9727936" modified="2022-10-12" version="1">MQQVKYPNLENIQYSQPSSSNQDLQQQLVNKTNENIKFIPVLISPQENLQGQAIYQQYSQYQQHQVAPYYQNQNDYVVPLQNTNYSNLIKDKEFPGQKLFLCGILGIYILWSIIFVILLFPISMLFVDKRNGSQNPISFILLILFSFTTILLAKLGIKKNNRNTLTSILILFGVILSYTCFYSAFLSTLASFSRQQFGLDFVISIFLAASVIGNFIFNFILLIYLVFAEEKLKMIYPILIELIGILIFSIIYHPIFLFCILFMLPYSISLMSAFSQIIKGRFQLERNQVFAGAIASFYSQIVCCTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N558</accession>
    <name>A0A8S1N558_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0810110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N558"/>
    <dbReference type="OMA" id="GTINQFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8088507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="432" mass="50033" checksum="D935FD0D5C37A72F" modified="2022-10-12" version="1">MQIGQVILTTLYFIQGLIVGLPGGAIQVLYAHEKDIGIFTLCTIPWAFKFVWSPIQDYYFIEKFGKRKTYIVPFLSLMGILLILVANAEHTLLNLFYYYIALMICLSTSDTAIDGWSVTLLSNVAQQSVCQTFGQQVGWFISQSIFILLNQKNIINFAQYQYTLGIICIIMSIIVIFVKETNPDEVIHYQSLFHQIKQVKGLYYNKNLRFLCLFIFLQRNGIAFMDIAAPIVMIRAGMEKTFISQAQIIIFCCTFWIPLIIGYFISGKKKENALVISALTYQTINMAFFLLDYYEYRKHPLTEGTINQFQYLFCFQLLVHEIFKKIFTVCTFSFVAEIVEPQLAGMQISIISSLHNVSRVILDPLILQSTAYFNIYALVLICVFYQIIVLRRYGDEFKIKQFISKENWTLHTQDTIDDYQELKEIKGFNNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9S3</accession>
    <name>A0A8S1N9S3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0840001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8089560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="351" mass="40083" checksum="891DA6B56636E77B" modified="2022-10-12" version="1">MIKQVPTNQNLSEQKKVTFQTPKPKSLIKHQKLRSPSGLGLGLGFGVGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXINIIIQFQNNNRLYLKIDYNKFDSQYDLPSTQYCSQFWNLRLSDTLKVEEIDTCNDQNINPFDGCYYQQFDCREGSSNCIKSQCIECDINWFYYPQNCYSYCRDGTIVGLEECDDSNSIHYDGCYQCKFSCPLNCIKCQYGLVIMTECLYINHHCLLKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAJ8</accession>
    <name>A0A8S1NAJ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0840145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NAJ8"/>
    <dbReference type="OMA" id="YKNQEME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="192"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="474" mass="56299" checksum="AE52445DE1B73A22" modified="2022-10-12" version="1">MSFQGFLVKKTIQQFPIRQKPVYYDDFTDQNFVLFINKKLADLRFQSGSNDLSAKRIMNLKIKIPKDKNTEGQKIKRKASLKRKSLSVQKDDDADKKFRYKFRSPPVKPEVRLASYLNDELLRQHQISPLYQEYKLANLKANLVKLQAKKSYQLNELNHVELEDDEQVKYFLTEICKNHPEIDVGKLLKVDVEKKEQKLDNQEQILKELIQKRIKQEELEQLMKLEQQYNQNLVNIYKETRYDQFEPEFKKQGQINMCKIKKQKEIQDKMFAEIFKSKSHRTSKNNTTKHFFHTKIKTPESKHKVTLSQQFTPSNNIFISQPTTVPMSPQTSMHQELKPSQMTTSIGTFNDSRSPINVPNTDFSQKIKTIMDKCELFEDEHKISNQILKKRINLMGRELNTYFDTARSRYKNQEMEIDTNEGFDRFLQENQFKRKLIEFQVSQVQNLYEVLSQKAKRELTNNFMGSDLDKQKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDU2</accession>
    <name>A0A8S1NDU2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0860215</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDU2"/>
    <dbReference type="OMA" id="NDICLRW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001344">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="28"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8090798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="400" mass="46710" checksum="72303A5450DEF5FA" modified="2022-10-12" version="1">MLDILLSPKNIQKPSKLLPKKIINDFVYHLEDCLGQGEYSSVFKGQDRRSGQEVAIKVIENSKLNSNFSRQMLSNEIESLKKLNSPYILQYINYIYTPNNQYIITEYCNEGELKFVKNRSDQQLLRIFHQLLQALKELKSKNIIHRDIKPPNIMMHNCITKLADFGFSVNMNSLELQKSSFGTPLYMAPESLLNDMYSFQSDVWSVGITMYELIYGNVPFYHSKESELKKIIRDYVNNPILRIPQSIFIPLLKGMLDPNPKTRFTVEQLLSMISIDQNTQNISQSEINFLSNFAQNLQHFETNINDICLRWAILKILATQYKMNGIAQSQINQQINQIQNTTNFYMIKDQKMIDLINSKSDAQFVYILRQILQQKINCQQYKPIVQDILQCDSILFHLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEI9</accession>
    <name>A0A8S1NEI9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0870114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEI9"/>
    <dbReference type="OMA" id="AFRIMML"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="62"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8091068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="414" mass="48514" checksum="C368771D6F82CEF9" modified="2022-10-12" version="1">MKYHKVKWDQVPIIDEASQNYLDQLNNRNHFHNFYTNQWFKRRSDQVKPQYLYYPEERKLQMELKQIFSTLDCNNEGVLTLAHLFILFKHYGYGVSVNDLQTIYQFIDYNKDKALSLDEFKGLTENQEALSAFRIMMLKILKTLEYSEKYVPLSVGAMLSHISYLVQREKQLKMILSEQSIAIKFQAFRQLIQETEKKTIADEAYQLFLKAQAQFYRKLKRQNSVDLTIKIRKSFSTINGLQPLLFRNMLMKDASELQDLAKLVQIQSKVKYDTFLTVTESLEENSLSNIESNDLELVVKQAQKIGKLQANQQIKELLTQRELPKVVSQHKKQLSVVTQDLPPIQFNFKKCHSTKTKSLLERQLKLLKIIPLEELSQFNFLSSSRSNKNYSSTQEHSQGGWSNTILSSKLPNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NK69</accession>
    <name>A0A8S1NK69_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0900223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NK69"/>
    <dbReference type="OMA" id="DQIETRY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="4"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="123" mass="13741" checksum="444DA66A1A677499" modified="2022-10-12" version="1">MDLLDGHLSAVLSVCFSPDGNTQASGSSDKSIRLWDVKKSKEIDQIETRYKNLLTQFQIPLKNSSLLPNVNPDRTILRICQNPVLEASGTLIMQGELIDHQGKNLKPLFKSKGSCFLEDLQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NLP6</accession>
    <name>A0A8S1NLP6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0920106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NLP6"/>
    <dbReference type="OMA" id="VCPEKAR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38150">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38150:SF1">
        <property type="entry name" value="PFU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="381"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8093072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="589" mass="68862" checksum="8B305971856C557F" modified="2022-10-12" version="1">MSVIRERHPLTSLNQLQNLFGKTKPQENTHSVCLEKTRYTSKPKQKTEDSRREVSNVQKSQYTLQSQKLYTNHLDNIKSSRAFNVKSQDLLLSPERIESLQQDNKVLEIQNDKKRILKYSVEHSNDTNHKLQTQEEQPKKIEKSQTILLKNLIQVKQQRDTTEKLKNSKREKHITSNQYENNGTKLCKTQSNTQNQTINECKLSENTQQCFIQRFIKDFYYFLSNTENVRISWDYSKIYENDFQQILQGLGFGESKDLWNDFTNGSYILSRNMLIVLLAILNVPVQQIPLYITKDDEQVPMASHYKNDENGNLILSSQDCIEIHNKYKQLYLNTKLIHGNGQRRNIEKSKSPPQVINLLSNRSRDMAIKKKNNLNINEWFAQQEQKKKDNIEKLKSQLEQQEEINEIKKQTIKPVAIDLTQLSKPIKKQQDKSTIDLEFENQQQFCTFSPQINPMKQFPISTASTQYDIDQQAKRLREARLRQKTLEKLKCTGNTTNNESRTQIKLQTEQSKSQEPKCKIIKSKPIFLNFPILYIDVKIDDAKTVRLPVFNGDNSESLATKFAIDHNLDNSLEERLKDLLEEQITSVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NN48</accession>
    <name>A0A8S1NN48_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cAMP-dependent protein kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0890070</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NN48"/>
    <dbReference type="OMA" id="GHIRITM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000039">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000006">
        <property type="entry name" value="cAMP-dependent protein kinase type I-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0114">cAMP</keyword>
    <keyword evidence="4" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="128"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="251"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8091806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="374" mass="42699" checksum="1251EF0C03BC37A3" modified="2022-10-12" version="1">MQNQDQEYLLNIVNPILEQLALYLVKEKPDNVTQASIEWLSVTGAQIENGLHHNQHLHNETVESSDDTESDEDDYELYANNPIRPDRASVSAEVYGIYNKKTNFKPKIVAKSHLQIQKIKEKLAQSFMFSELDEHDLRIVINAMEVIQCMKGDIVIKQGDDGDNLYIVDEGTLDCSRSKAGQDSVHLKTYKPGESFGELALLYNSPRAATIVAEENCVLFSLDRGTFNHIVKDAAIRKRERYEYFLAHVELLQELDPYSRSQIADALKSKNFNIGDYIVKEGDEGDIFYFLEKGEAVATKVLNQSQPAQVVYFYKEGDYFGEIALLRQAPRAASVIAETPCTVVYLDRETFKRLLGPLEDILTRNFKKYEKYMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NRM7</accession>
    <name>A0A8S1NRM7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0830201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NRM7"/>
    <dbReference type="OMA" id="NTKACYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="24246" checksum="10985507BA999701" modified="2022-10-12" version="1">MNQLVPIDSTLQANLERLSYLMAQKKEKKPDFRDQFQTHSNTKKIFVNLRAFVKQPQPPSPPSQNQKKEQKIPSQTITTQTQTPKTPTYRQQFIRPQSLAQLEPCIRRQEISTSNIMSERTNTKACYKNWVGLSVIERNDLFLKKKQQKLKKMREDKDEQELSKCTFSPHFYNQIHIERPSSCFQNKSYQDIHKLRKNQNEKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTK6</accession>
    <name>A0A8S1NTK6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0970018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTK6"/>
    <dbReference type="OMA" id="YLSQEIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="257"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="640" mass="75932" checksum="6BB5C573916C2637" modified="2022-10-12" version="1">MSNLTFSGMRTLKARVNEEDQNKKRHPFDLQKYHHILDQISTTHQFDMDLENRFNQLDQTEQSRPTIDKSSFLNKQYAKYIEKGSDLQLSKVDCIDPEDDVCTHKQTKSLLSLIKGRPIKFKEFYKILLQEYQIEFQNEEQKLQFKENLKNKIVTVDLNIVSLNALQLCTRDKGIKGLIQEIAEETYQDMNRKEKQKINNEIVDELENVKQLLEKKQLMQIKKEKELVEYEYSLQKFKSEMSNSIQQVYDILSKMMEEQYQQKLSNLNKKFSNMEYQLKSKVKLIELKGSSIQKNNSTYSQEIQRLKQRNAILEKQVESQKIIILELEKKLSLEKDNTIQMQKKLEKINQKLLEQKEQIKEQINQTEQNESVQSLKQQQIQEQSKLQGEQQKKIQNNQDNSNKQIKVFQSVFESIFLGLDQLQIQLNIDNNKWNQELLPCCLQDNLIDLLGNLMIIVINKQGFQQIYKLLLQLSYFYFKNTCSSINCQQIEELIKNQKKQQNCKYYIGIHESKNKEFILQLKALLEKKKKNQTLVAFILLLYDRDISISIKYLSQEICNEDVQKLILDNGLLDVFVIKMIDDRNMVQLLLEIIAQGQFQNQFLQQLTKHFEILLTLLNCQDLEFCEQLSIVLQRDQLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NV37</accession>
    <name>A0A8S1NV37_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1030121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NV37"/>
    <dbReference type="OMA" id="ASCCCDN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="236" mass="27669" checksum="32DE4472591018A6" modified="2022-10-12" version="1">MLIIINKRNQNRKTTQKLKYLDSVQEQPRRASCCCDNCGKLTHFQQIYQNSPLVVQQHYVQKQQQSLLIECKPLKKMISTSFHLSNKNTTPIKKRRHSCKCQECGQETLFQKNTCYKIPLRQPIKSPHFKTFIIKKSLGKVFQEFSDVKKTYPLTSRSHKDVLIKSPILVNRLKTGSCLLPLQNSQSFKYQVKANKQLVELKRFKNGNSSEKFKYQVNKHEHIIPTTTIRLKTIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZW1</accession>
    <name>A0A8S1NZW1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Golgi apparatus membrane protein TVP23 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1030162</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TVP23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZW1"/>
    <dbReference type="OMA" id="KMIWWID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008564">
        <property type="entry name" value="TVP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019:SF7">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05832">
        <property type="entry name" value="DUF846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8097299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="218" mass="24969" checksum="A33555542F4D3DD8" modified="2022-10-12" version="1">MEEDLHFKIQMQEMPTAPPKPLIQTSTDEPTKLSVASNSESLPLQTSNYDLNRASHPKACIFTCLFKALALIFYLIFGESIWSFILVIIFSAFDFWTVKNITGRLLVGLKWENIIMEDGTSKWEFYSIPNKQVNAVDKTFFWTAQLGFTLAWAVFTFSNMVSFTLMKFVIDVIGLSLCWTNLFGYYKCNGDHKKKMKDLQGDITSKVVTQFVGQMMSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1E2</accession>
    <name>A0A8S1P1E2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1010150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1E2"/>
    <dbReference type="OMA" id="NFRIFAR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="223" mass="26305" checksum="D9856829A4E7E55F" modified="2022-10-12" version="1">MYNKHHLSTKNLDGPNLDTTQVRRKSCVCDQCGETALKENQLPNQLCEIAYHKKARLEDLFLNKLDGAKKYIRNDHKRKNFRIYARNPLFILRSKPDLDESMHQQTSPTKTNSPEQQRIKTEQPQILHSRSHKYVPTSNFFLKNIKQQVIRQEKTINRLIKDRKTIKSVQFSSSGSVPINKSHQNNKKIYLKQLKPLPKQQQLQSKFKCLIRQFFNSSKAKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P4P7</accession>
    <name>A0A8S1P4P7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1050135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P4P7"/>
    <dbReference type="OMA" id="NIHINGY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="191" mass="22339" checksum="80DCA01B579D6580" modified="2022-10-12" version="1">MCQKQNEKNEEMLKQLKQTMDWQFSQSLTFSTIYKNSNCSITQNGKVIETSYGNWQCCMCDQMIPKNGLIQFAIKIIEISSIMIGIGFRDIVQSRNYINCYDIGQGTYNIHINGYCYNHDQQDKNNKQIAFPFLTNDIIIVEVDIQKKYVKWTKQSTNQSFTLNIDTSKDLYPCVHLHSRCKVEILNQVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5V8</accession>
    <name>A0A8S1P5V8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1070010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP97 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5V8"/>
    <dbReference type="OMA" id="EKSYDIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038791">
        <property type="entry name" value="Cfap97/Hemingway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029488">
        <property type="entry name" value="Hmw/CFAP97"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23035">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 97-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23035:SF2">
        <property type="entry name" value="KIAA1430 HOMOLOGUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13879">
        <property type="entry name" value="Hmw_CFAP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="237"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="237"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8098376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="296" mass="36027" checksum="0705381DBC719E9A" modified="2022-10-12" version="1">MRTNHQYNYNYKLDQIFSREKSYDLHLKKLNEIKSKKSHSQSRVQHIENLEKRNNIRQQRRRFDLSEQSERINKGNSQLYYKITEICNRPMQQEYYKYEDENLHHPHNLNLTFRKLQAQQIQHENIKLADRLMKQEPVLKLDEYSHQYKENKRLMQRLQRYQQCQQYVNSIRNNLTQSNRDTTNHSQISCKKKEKMQLQPILNVKNKSIDASELDKINYLLNQEDESTLKELLQLEEKQQQQDDKNQTKQELPEIPQLNEESQIETIRQDEIVENQIQQQIDNEVKLQSDDDVDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7D1</accession>
    <name>A0A8S1P7D1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1080001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8098788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7D1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="48319" checksum="BB79E3981402C051" modified="2022-10-12" version="1">MQFLIQKKNLQEWEQDKIFNLYHLKILFQILLSYWLLFLKFFIRQQYFVSFNSQFDIFSNRNLFQQDFKQINFNKFNSKFCALNQQETMIILSKNKYQQFDLVEQKLVLIELETQKVISCFEEVLDQKFKCIDSFSLTMNQILLQVIQIQELNYGILNLVNSSLSLKRILQEQICLQFQVEQYQFKIVIISQSYGIQKPQNSNNLKWMVILILLIKYVFLRWFPNGNRIIDFHPYFLIHLNNQYFIAKGEQEFFHIWKFNTKYITEFHNKYLCQPIWQQQFSLYQNLLIWQIDNSKIFILNLEKISKQKQLPFQSDSKIQSRTIILSTNLLIRSNPFEFISINNKLELKNEQIVGYQLIKQGKLHFVLFHQCWLYMVNHILLISGQLKKNKNSQFIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8P6</accession>
    <name>A0A8S1P8P6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AraC effector-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1080176</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1080177</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8099116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8099118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8P6"/>
    <dbReference type="OMA" id="YCMYYDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010499">
        <property type="entry name" value="AraC_E-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029441">
        <property type="entry name" value="Cass2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053182">
        <property type="entry name" value="YobU-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36444">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN YOBU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36444:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN YOBU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14526">
        <property type="entry name" value="Cass2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00871">
        <property type="entry name" value="AraC_E_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AraC effector-binding" evidence="1">
        <location>
            <begin position="8"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8099118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="173" mass="20081" checksum="B2932B32910C1624" modified="2022-10-12" version="1">MDPQAQVPTRTVVELPTFTIVGLVRNIIQKEEQDPKTGQIGKAYEEYFDKQIHDKIPHRLYPKRTYCMYYEYQNADNQEEIRYKMVLGEMVSQVTDIPEGLVAVKVPAHHFCRFDCGPGPIPKVVIDAWQSLPNLTSEEFGGVRSHDFDFEVYPEDINLKENIKFELFIGIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PA58</accession>
    <name>A0A8S1PA58_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1110069</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8099980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PA58"/>
    <dbReference type="OMA" id="YMIKEMH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06174">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF9">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="394"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="8"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8099980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="489" mass="55726" checksum="40FABEE4BDBFD69D" modified="2022-10-12" version="1">MEFKPRWIFCLLLCTNIIINLDQGIIPAAISQIEESLELTSEQLGYLGSQVYAGVTLVCLFGGKLFLHFNSKLIVMFSYLGMISSLTLFPLNYGSSWPYYIFRFLTGCSKAPMMIYFPVWVDNFGDEQKTIWITILQGVIPLGIFVGYSLSSVISSVWQWQVAFYAQVIMLTVCATTFIIFVQNKDFDIKKSTKNKFGNKSINEDQQNRSLLQQSPNKTYWQMMKELYSIKLWLCCTIVISILYFIVTGIQFWMTDYMIKEMHQEKQTVNIVFAIVSITAPVFGCITGGLIAQKLGGYQRTKSLYVCVLYCSLCCISAAPVPFTETFWFGALCLWLLLFFGGAIVPPLMGIMLSSVPKHLKAFANSSTTMFQNLFGFLPAPSIYGFLMERYNSQVAVFALMYYSFVGLFVMLLAVYFKRMQNNKKVGFEEVFEQQQDTKSSVLQDEENFNITVRVLQYGDAPLVTSLTPHIEDQIPNYDNPEDGNMKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBV9</accession>
    <name>A0A8S1PBV9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1120079</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PBV9"/>
    <dbReference type="OMA" id="FIICHIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="407"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="695"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8100374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="782" mass="93321" checksum="66792E044D4FED68" modified="2022-10-12" version="1">MTTLSLSSLQQQRLNKNQELRKIIKKVGMAQKFINQLQNYAYQLKNQRSKYQYRKRGLLPFFPDDIIYIVWGLGINLCTDLAAILYPIEVAFEYEGVGHQLTFILQIIFWIDLLMNFIICHINKQLDLLHNMKDIANYYLTTWFIIDLLSVLPDFGLEGLKPIKLLRLLRFFFYERRVEYNQSIELLRKQQSLRDELIVRDEYNIDLRIKKLMKIFLEMIILNHLFACLWIWICKFNVNQNWMERSDIKYANDYTKLIYAFFWAYQTITVIGYGDLEAHNFDEYLLSVIWMLIGVGYYSFTIGNITFILIQSNPNQEFDDQLLNLEDVTINMPENIQNDWIRFTKYNIQRNPFWAEDSKRIISELPHQLILYTVAAVHKDILRIIPFMSNDINFSGSILPHSTLVCYQKYETIYHIGQSANDFFFLIKGDIRLCDNYGETLVRVMEGTCFGEIECIENSLRRWSAHAIEESVVLMCSSNYFSQFLEKESPQFFELQQMYKRRKILIIEQARIKRQRQSKLKRGSAINDFHQSNNKRESSQNLQELRFKIDLREYSSVQQDLMLQIVGQKQARNKIIREKFINAINKIRYYVQRSSKIKRISIHDPDYKIIRNLINNRIKDCAWEKLIQKKVGQKLLKNFEKVREDENLIKAFLQKSKQESNERLSRKKLIHIIRNILKFHKKEHQGINKIDIFYNKFHQLIYENAKNEKQEERKKQKQKLESIKRCGALEDQMKNLSSNLKKLVNLQTSLSMSIFEINQQEYDIDCLIADIKKTLLQAKIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PF19</accession>
    <name>A0A8S1PF19_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1160025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PF19"/>
    <dbReference type="OMA" id="GCLCQKL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="118" mass="13886" checksum="E4C6BF9F8C00153E" modified="2022-10-12" version="1">MADIVFNYINIINDQKSTEIQVKDSGILKKSIKKGKGCLCQKLRQKKKYKIQSPEESSNECDGWEEEDNYNRKSSKQRNQKEFVDLVQYASKLYYMINIAKQQLKELEETMQSNHAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PF26</accession>
    <name>A0A8S1PF26_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1170022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8101912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PF26"/>
    <dbReference type="OMA" id="CTPKKWT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8101912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="148" mass="17698" checksum="22B25544E258DD1F" modified="2022-10-12" version="1">MNNYEIGIQRFIEKIREQIRLKEKEYLQIEKALIHKIVHKSPQPARTLDLRPNYFLDVSIKPKHVKPKSNPNNLKTFDEMEPDVLPYLLQPLQTQNHKINKRISLKIPTTVSEQSKKQEKIENLHLRQFYRNCTPKKWTVESSDEIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PFR3</accession>
    <name>A0A8S1PFR3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1170081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PFR3"/>
    <dbReference type="OMA" id="TIIDIMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="412" mass="48333" checksum="7025BD3DF49F5B6C" modified="2022-10-12" version="1">MRNRLSASSFKNQAKQIPIHSFLTFNRKRSCECLDCGLHNKKMLYVTTVLPQFQPYQHIITKLKEQNEILQQRKRMKLRNKRHLIQKKTIIDIMQQQEHQPKKRQNRHHNTVFYNHQASIPNLSSLYSIEVAQTPQKPISRSSIFFHRYNSLSPRKLFTDKKNSKVQSFSQRLKTFYLPITAPFESQLIFRLELVEDGKIKLIKQTGSQDDFNINIRLNKFEEDDKKNEDQSTQNLANLPTLQQEDEEEDDQTDDENIVNIKQLTTQNPKQAYKNILKYNPRTLQSLVDKSQLGQSKIKDIKIACPKYLTQNHVEHCHTSITNNMSPFNNTINRCSPIPSIKLLEEKRKKPQIKLLPLEIKPFQQFTLTNISPTTLSRTKPSPKKGPQRNIFNAPKKKHQKNITFGQLLIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGS8</accession>
    <name>A0A8S1PGS8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1180044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGS8"/>
    <dbReference type="OMA" id="CQNKEAF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="375" mass="44133" checksum="DC776C041CEDF162" modified="2022-10-12" version="1">MRARKYFVLVQENLQTQFYKTEKYTQINLDKNPNQKKFQFQDENLAKKFISNVQSCQNKEAFEKLEMDYEISKQLAKYQEKIKSCKTFSDNENLDGVSINLRRLTYIYVLWNKNSYELPRMYFTQITAQKNSTSEDQLGKFSEPEAALSYIREILETLEIKRKLLSDTNASTSSENNDKTQDEIVKEEKELNFSNLGKLQQLDQQYSYFFPKNYLDKYQSVNIKPEESYAIQCSSFCTLHFHSSVGINLINYVSGKNLRTQGLYVGHQKPYVLGLLSVLYGLRYSLFLNLPQITLQVWKKQDFEFLNKMVQPPYPQQQQAMAAIDGYSTLLKQVKFELTEYDLCKSSYVSAVKEFEKYANVKQRSKKIRKIDKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PL94</accession>
    <name>A0A8S1PL94_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CSC1/OSCA1-like cytosolic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1210116</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PL94"/>
    <dbReference type="OMA" id="MGELECV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF83">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="422"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="471"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="509"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="576"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="4">
        <location>
            <begin position="198"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="3">
        <location>
            <begin position="375"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="727"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="747"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8103574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="760" mass="86817" checksum="15D12F59E5737E6A" modified="2022-10-12" version="1">MTSVIPYDQETHQLNGISQPNETMIEDYDEFRRPPLLDICLKHQSATQFAIADAKVDLNKNNLCPCCGQPIILTQLPLTASILDFSFNGPGVSLYFDFLMFSSILISTFIIICGIYDLIASSLGDTCHKLNVMGELECVENFYSKFAKSNQDNQHPDIAGSVLNFITTLILIILLFLYRKRINILANKIDEKSILASDYSIIVENIPRDAKEEEIKEFFSKINNLQYKIQKICMGYQISEYLRILKQKQEQEKTFTKILECERLGKPIPPRLPNKVDLTQTLDQLANQLDQYEDNFQNSFQFSGVAIISYNTEDEANAVCKFFKATRLHLILSQVMRVIGKKDIRRFRDNVIVVSKAPEPGDILWGNLGVTLLEQYKRKFITNLATIFLLGICFGILFGLSYGQFSITDTGKGEELSQAKLVAITILGVIASILISIINNILAIIIRKFAELEAPTTKTEYDISAAKKLGFAQFLNTAIITLIVNLVIVKEGEKSYYAVIKQGGLNQDVMLIFITNGIMPWLMSLLDPFYLYKLFMRYQIQLQGQQCNVTQQEANEYFAGPMIDLSKKYAQLGKTLLFTFFYAYLLPLGPVLSLGSILVIYWVEKILLLRRDSKPAPTGSEMAEEMIDFFGEFTLLLYAIGCLVWESILNDAIFPLTWVQFTIAVLNFLIPIDTIFDIFWKSEDISTQEFYETQCYKFWDDYDRRNPITAEKAIETFLKNTTALPQQQQVDGRGNIVQKNQEYSKQEKKKKAKNKHNKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMT5</accession>
    <name>A0A8S1PMT5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1240096</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMT5"/>
    <dbReference type="OMA" id="MTKIFKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8104617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="392" mass="46164" checksum="5F3353E5C5CE3070" modified="2022-10-12" version="1">MNHLFYKNVLIQILLNYTRLEQLLINYKVLVAYNFIYLIMEKLNGTTLDVILKEQTLDELQIRNIMLQVLNALAFLHRKGIIHRDLKPENIFISNGNHVKLIDLGLGYQIVCRGIIGQQVGTPYYIAPELIKGYEQTQAIDIFSLGIIFYQMLCNNLHPIWTEGQTKKDYYKTLCHEFHIKYPSHLSEMAQDFIKNTITHSPIDRMTAEQCLEHPYLLGEERKSHPITNKEIIQRYKFIQKFQLVYLYHQICSQIVKALQMTKIFKQQCYEGYSYLKSQNSDEINEILLHDDAPKSQRIHTETHIIVRHQKSYYKPQSLKTIHLTKVTSSQDLKSIKNKMLNSNRCYNLTSRYKSSLDIQLPKVNSQKRINLQLPSISPYQTRQSSFIFRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PND1</accession>
    <name>A0A8S1PND1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1250110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PND1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="460" mass="54497" checksum="F0C03C7BDD4D05B9" modified="2022-10-12" version="1">MVLLIIINQLDITKILDQLQGRLQTCLIIIMRNLIYYLKKEEKAVIKNQNIPLYKTISINNCKKQRKVLYIRDIKHQYIQQIFLILETKSQFFRNIKYQNHVLQFNHQSSKITTRLLKQSKLKLSNLELKLTLTPQLILLLVINPHYNNTKWHLFQSIFWIPQKVFFLTLKFCIKIAYYNQLNKKFIRGFEKILINKTELIYILELLFFLNQWLLKIMKNISSSQNLQALSLIDSYYVVLNDRKRIRQTTNFQSAQRKFKEKSVKIRRKSCHCQLCGEMSALQFNMMNVPFQKKEQIIEKVKKQNQETPCQLKERRSIFYQLQSLKSTENTNVRLSLQIHGISKNFCRPSTIKQLIAESNQRSRRGSLYGSYQGQTSTSDNHSVKSLSINIFQDQSPLKTQSPNNETITNRNTILSSKQNLMKNTYFSQSVRSLKTTQQSQFNKYKGCILPKLNQKKQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PNL2</accession>
    <name>A0A8S1PNL2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PPRIM_AZ9-3.1.T1250150</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PNL2"/>
    <dbReference type="OMA" id="ANSGMDK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000277">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="20">
        <location>
            <begin position="316"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="20"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="679"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="40"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAD8104947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="759" mass="86900" checksum="EBC828FD3F6E754E" modified="2022-10-12" version="1">MLSKLARISHFRPFFAFCDQKPPQGFEKFKRKPKQSNEPSEQKIENIQEEKVQENREDKKQEENIKKERPIEPQFEQPKQEQQQNINKRFSQEQYKFSKRENTNNFGVKQKPTTPSQDPNDQIKQYFFMFLASVPLAYLVYKAYEEGENYISFNEFQRGYLEKKFVISIQVEKINGEIIAIIYTIEGKKKLKIMDLDYFLQKIDDLKNEQQIPISFQNQDETQETTANITKYASILFYGGLFFIMFQIYKNMTKSLSGMAGDMMGGMAKMKSKQFEQKIKIKFKDVAGQDEAKGEIKEFVDFLKAPNKYKKLGARIPRGALLTGPPGTGKTLLAKACAGEAGVPFFYVSGSEFVEMYVGLGAARVRELFKQAKSKAPSIVFIDEIDAVGKKRNSKGSKNEERDNTLNQLLVEMDGFGTDSTVVVLAATNMRDSLDTALTRPGRFDRNIEITLPDINGRKEIFLVHLKPIKLDPSKTKEEYAKRLATLTPGFSGAEIANLCNEAAILAARQNSKYVTSFHFEQASERVMAGLEKKKFMSEEERKIVAFHESGHAVVSWFLAGGDPLLKLTIIPRSKGSLGYAQYLPNESNLQTMEELKDKICCVLGGRISENYFFKSITTGASDDLKRAYDYANAIITKFGMNETIGQIGYQDDQYSKDYSDKTNEIIDEEMQKLIYQCTKRTEELVKKYEDKIKALAELLLEKESLDLQQIINLLGERPFPPKSNYKAYLDLKKVEQTEIKNELPQEQNQQQLSQSEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTX9</accession>
    <name>A0A8S1PTX9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1310026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTX9"/>
    <dbReference type="OMA" id="YGTANEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035740623" evidence="1">
        <location>
            <begin position="16"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8106512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="132" mass="14533" checksum="9886282103851BA2" modified="2022-10-12" version="1">MKYIIALLLVSTILAAIPTKVTNCMANPKIVFTEATFSVTPAKGVDETITLFGSANDHAELANVQLKAKWNGIDAFEDNYPEDEVYDKGDLVNYQMTQNFPTFTPSGKIVVQMWFQNAKGTNFACAEVGFVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PWL0</accession>
    <name>A0A8S1PWL0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1340095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PWL0"/>
    <dbReference type="OMA" id="QIQIYRS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="60"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="413"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8107657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="464" mass="56007" checksum="6C12E60EE4CD408A" modified="2022-10-12" version="1">MFQREQRNTTNSGYRNSSNAYVMAMKALQEKIKVMETQRSDTLLPTKTKSNDEEYLLQQISELRNQNETLTMQLQRQQNEKENISNYIYQIDQLQQERLVHLKEYQDRVTDLIKKIEDGKKDRQDQQNQIEYLQRQLQAYKSNERGLMQKVDQQKQEENSKQLEQIMELRQRVESGEQYAKKLEKKYEKLQTEKHQLEADILDYKERCPVFKLQEYEKQIQIYRSQLEEKEKAYLRMMEELQMQRQQTEDQLTKRIQELLNKSNEYQSNIHQLTIELKDITLKYQQLKLRLEYEEKNNQQNKKRLRNISQSEDPEFDMLINNQYQSNNQQSNNNNKVSKLNLDFLSPQITSPRFEQSFVKSSVLKQAIKDCLEDMKQTTPFKSDQHNLISQEQHHQQQHSKKKLLSPPLTVRNSEIESKLKQLNDKYEQLIRQAQKESDFKQKAQIRKELLDIAEQIKEVNRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q3T9</accession>
    <name>A0A8S1Q3T9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1400088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q3T9"/>
    <dbReference type="OMA" id="QEMHMKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="114"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8109328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="374" mass="43769" checksum="177CDFA59121D3A6" modified="2022-10-12" version="1">MSQQFQGRSSHGQLERKFEPAIENPKPAQQLRNIHVTGVTRDASKTIHYPKQPKIQIIKVPQRCGSNQTVCSVQGFSTLTYDDIRPKEKEESVINKIMRLFTREVDPCKCLPYQNNTCIKAKEWLGNYDADKLKTKYSNLLESKCEPKIIKQINLDVERTHPLKNYPQKLQDLQQVLIAYSKYNKIVGYMQGMNFVAAGLIYHSDNYVAFELLRKLIDLLQMNDLYSPMSPGLSKHIQLIDYLILTKMPDLYQHFCINGVKVDMFCASWLFSLFGMMIPIQKQVKLYDCIFKYGWTYIYQLIIGFLLYHQEILMSEDMTGIICILSQQNYRIDDNEFEVDWDELLDASLNVKISKTFIQEMHMKFDTKIQTFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q944</accession>
    <name>A0A8S1Q944_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1500041</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q944"/>
    <dbReference type="OMA" id="IREWDRY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="4">
        <location>
            <begin position="423"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="651"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8112112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="783" mass="93294" checksum="1F18193A1F1BAE9F" modified="2022-10-12" version="1">MNKKSEYTFEQQKQSQQKLQSIHQQCQIATKKIRQGLLEGGSIKMQNQEIMGIFNDIYKLTENPGKTQNSQNQIKVKSGESIEEQLINYFKGFLQEFITDIYQKFNEFKDEKESVLYIVSKTFHDYKAYSYWLYKMFSYLDIFAMPQINTNLSSTSLQLFKEGYFDKCNKLILNTILQFIQETRKNGILLNKQIKQLIQLFSVMGSNEVELKHTKEVGEYDYVCKKQQDASIFYREQFQSHLLQETQKYYKEEIDDKQKFTTPEFINWGNSIFKLELDMCIECYPKSQQAIENKLKMMLVKDQASRLINSPTGVGYMLQYDRTEELKQLFLYILRNKECICHLAKAYQNYFEQQGQIINNSIEQEQQQQKDGRQPHEAELYFTRILEIMEKAQNILKQQLYDEPEIQKSYSGAFMCVINKNEKSPMWLAIYTDIIIRSEKGVNEMETDKRLSKIVSLFQLLYQRDVFFRHYQKFLSNRLLNQQIQNLQLEKQLLQKFKGETGTNVLTQLSSMINDIEQSNRFASDQQISKNTKFDLNVFLLSQGCWPITTILDNIIKPPQIQNVLENYEKLYLVKHNGRILTWCLNMGQGELIYKIQTEKYYLNVNTIQMVAFLQFNKKDSFSIKDFLEMTKIDKIDLENSLIPFVCMKIISREKQDIEEFTDDNEVLKLNLGFSNRAKKMKLLPNQKMQPKRANKVKEQNQEELQQMEQLNKQREFVVDSQLVRLMKSKKTIKHHELLENCQSMISIFKPDVLFIKKRIENLIERDYIRRDEKDWNIYHYQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBA0</accession>
    <name>A0A8S1QBA0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T1520094</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBA0"/>
    <dbReference type="OMA" id="KAHRFDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15845">
        <property type="entry name" value="SNARE_syntaxin16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000081">
        <property type="entry name" value="Syntaxin 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF83">
        <property type="entry name" value="SYNTAXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="218"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="155"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8112693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="313" mass="36218" checksum="4FA459A8D3393BF0" modified="2022-10-12" version="1">MRQSVGVMRNKTHLLFKYKEEQKQQANRFKKTNNIHTSKPNTLLLDSSGESTASNFSIEMQAHNKGLPSEWASHYEDCLDKIKQVQEILKELQILGSKRLKMQFGDATALEKLIYENNQKATQKIMECEKNTEFIANYSSDKETPSDQRIRMNINRALAQQIQELTNALRNQQKRMVTMIKQINKDDGGNFLKLSEQKQQEMKVADDELTQAEEQMYDDIICERDQEINKLVTMINELAEVFKSLNQLVIDQGTILDRIDYNIDQAVFNVKKANQELKKAEEYQNSPLAKRCIIILVIMIAICSILLTIKYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBX2</accession>
    <name>A0A8S1QBX2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Heat shock protein 90</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1490076</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBX2"/>
    <dbReference type="OMA" id="WYLNAVE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000014">
        <property type="entry name" value="Heat shock protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="146"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="513"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8111860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="65173" checksum="86C4B871D1DF2F4F" modified="2022-10-12" version="1">MQVNENKAIWLRPKEEISDDDYKKFYKVLSKNSGEDPFNWVHFKAEGEVEFTSLIYVPKRAPSDMFDNYYGKQTTNLKLYVRRVLISEEFEDILPRYLSFVKGVIDSDELPLNVNRETLQQLKMLKVISRKIVKKILELFQDAASYDEEDEDDTEEGEEDDNMAETTPEEQQRLKDEKRKKKMDEYNEFWKEYGKNIKLGVIEDSSNRQKLAELTRYNYYINYYMKYIINGIISIYYMIYINSRLIQYISNLILSIYQILYLIFYFRWYSSKNSTELTSFDDYIERSKPGQDSIYYLAGENKEQLLASPIIQGLIKKGYEVLLLEDPVDEFTFQHLNEYKQKKLVNVGKGDFKQPEDNDEQRKKQKALKKVFQPLTDWWRKLLSENVDSVVISQRLIEDPLIVVSSESGYSANMERISKAQAYSSKGSNSQQFGKKIVEINPNHQAIQELLQRVKDDPDQETEEMARVLYEAALVNSGYSIPNPEKFASRFYKLFNSALGIDRDAPIKEYEVEIEEEPETSSEPTVDQDGTKWEKVNTDDAQWETVSDDKRDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBZ3</accession>
    <name>A0A8S1QBZ3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1540054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBZ3"/>
    <dbReference type="OMA" id="PVANEQH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="439" mass="50080" checksum="20BF9818591AB53E" modified="2022-10-12" version="1">MSEIDITSQENTLELPKFEESLPDIKPEPRKDLKYEIKHEPKPSFGQSSKDLIEQYKSKFNAFKQLAEDQQPLAMEPSVIEKSEKSDIWRSDAEILKSPKEWDWNILGSKKASTTSNQIQFDTHVPDPIASEQEHKAQIAQHRQMILGVLDQRVEPGLKLPSSHQPSKPVVNEQHSNYQNILEKFRSASSLDDLNLFFNTVQQRKVVEQAAPQQQLEDAFSNIDIRRNKKAETQIQQYKTPSKSQEEIFEYGSGNRTTSFFQKKPSAQKQLLQSPNLGNQFNYRPDQSVTSKVTQNMNFQVEVKDHSKTNKRKEELQSFMGKLGLGKYDTPKVVINLDSNTQSNSTIDRMRQYVNGTPMSARKNGGGLSDLINERSLNTTTFKQQIKSYHKSGSKPDQTGRLSADNQNLSEQSLKNLHLKLVHQQKNGIGNSGLKYARC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QDF6</accession>
    <name>A0A8S1QDF6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">APCDD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1560061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QDF6"/>
    <dbReference type="OMA" id="DYLYMGE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042425">
        <property type="entry name" value="APCDD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31021">
        <property type="entry name" value="ADENOMATOSIS POLYPOSIS COLI DOWN-REGULATED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31021:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_56, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035894520" description="APCDD1 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="47101" checksum="C69D0C7F3CC3BC1B" modified="2022-10-12" version="1">MFTITYLTKKLIIMTLVIKLVTSQTLDEIKAQIINDWKSIALELVPNYQGGTAYPEYQRRQWNFTSDSGFSTLIENFEDKSGSNRKISIQGQGEITFQGASDVISGAYLCQFNFSKSAIITLHTDESVTAFNGAQQGQDGITWVKDKPQDVTKLAVPALGKQSNQFFLAYDLIYIRDDYLYMGEVDVFGNEATLDNPPKGLCAPLIPFSDDETPLTLDELKESILGGVWQSLTKEVRPSKNNQGQLITTFQTRKLTFLSESNYSLVLTSFPGPGQTSPLLEMEVIGPFVWEEDASQVVAGAYFAKFSMSQLYITPMNDEMAANLNQGLPEGIEPFKNGQKANLTGKEFPALGMTNDSPDYESDMVYQRQNRLYLQARPVDGGMLYPKERRTYSLQRDLIDPERSSFNAFLLLNLIVILML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QHN1</accession>
    <name>A0A8S1QHN1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1600046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QHN1"/>
    <dbReference type="OMA" id="SQECNIP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="293" mass="34295" checksum="C2826EA7BD08B567" modified="2022-10-12" version="1">MLEEQTREIRRRRIITQDQMINNKNTDKQRRSNRFENQKNRDGSPAQSNSSGSQNKTKFSTYRKATNKSPNSIERYLMSQECNIPTDHSQQDNNTKHKIRDQSFKIGQIYFKQENQDLQFQEVIEKLHNTSDIKIESPHFQNSKKNLENKQSLDQIQSIKDPSASMKTPTTTALEVSHNQTMSNPLLNQHPSISRDSSPTKISHPITQVSIGPISNKQSLSVITDQQQLKNRSRTADLFDDQKSVTIRSVLKKKQKNNDSFRTQQQESRKVRFDLPNSHYIRERQRQQPRLYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKC8</accession>
    <name>A0A8S1QKC8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1690034</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000178">
        <property type="protein sequence ID" value="CAD8116109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKC8"/>
    <dbReference type="OMA" id="GCIMHEL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="28"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="843"/>
            <end position="1069"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8116109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1095" mass="128712" checksum="82B24EBDEB9D9C91" modified="2022-10-12" version="1">MYQQNQFNQINVGTIISAPSGQYPNRQFKLSKLLGQGSEGSVYEAIPINWGQNQNKVALKFSTIIKDNAKQFYKQIIDYQNKNENYNSSNYQASNIIRIHDFFDFSNYFVLVMELGDIDLYKYIEQNKKTLTIQQKQSICLQILQSIVFLHKQKLVHRDIKPENYLFIDQQVKLIDFGLIKYFNEDEKRMTKSVGTKLFQAPELIEGKQDYTMSVDVWAMAFVFYEIIQGQKLLETINTHEIEKQILNHKLDQSSLFNKIERLMISNEWKQTIKSMLHPDPYQRITANDAQDLFKKSEIVQPSVQAQQNQGQLNQFQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQQNQGQLNLQQSNQGQQNLFQQYQFQQKQGQQIFSLQNQGRLTQKQQNSGQLSQHYSSTKDLVYSLNETKNFIQILKNSINIKSLQQDKEQTMQIIFQFENLIAQVEKNLGNQQQVMQTITEQLAQQLKQQHANQDYDAIQNKINKNNELISEKLKIKKALENLEKSKNQLEQYKNLNYQLEELIKENQEVEKQIYYQDQLKKKISELTKKINEEKKYIDQNQISILENEIKIYETENLKQETQKQKYDKLQQEIQNLKNQIEKSNNEIQADKFDQFSSLYSNLQEENQKLENKKNYFLQLPNQIQELQESIQQFTAKIQIGQNYQNELNSLKNEEQILKEQQIDIEQLKKQIEEKRKAIENLKIQQSIQQQQQQQNIKLDQELNKFKIYQNNKQQQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QKV3</accession>
    <name>A0A8S1QKV3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1660049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000175">
        <property type="protein sequence ID" value="CAD8115804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QKV3"/>
    <dbReference type="OMA" id="VGPQDYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38150">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38150:SF1">
        <property type="entry name" value="PFU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="48"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8115804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="464" mass="54070" checksum="9589D663D8671585" modified="2022-10-12" version="1">MINLSDREKELIEEICAKNAKSNKKSNKIEQGNQKLVSQSSTKILVSRLERKLNETLKEVDEQNSGQINFQQLGRLFTLLDIFQAISYNQDYQLEAQGLNNPMQKQQEVDLHEKAFAIISNDEEKADIQAAFCLFRIILDPNQLEPSKCAILIKEYLEKISEKEIDYEAIFKFCSEFQVYQKTRLSGAKIGYLKASLAQNLIETYEKTLTFKPTINPISEALQQQQSGKKENQESSSQISQGLAESRINELYKKKELSNQKIEFLKQEKFMKDMQQCTFKPQIISKKELPNVIDRLYQVKNRQEIEERAKLAEQEKEESEYSKCSFHPQINYSMPESQQTGVGGYWEAIERIRRANDKRHLQDLKLNHKPSGENYEKVKNQPFSPPEMLQRRQFKKQLPILYIDIKLGPRKVGRLALRRNDNVEEVVQSFCKVWGVGPQDYELLVNQVKENLQNVIQETDESQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QMZ7</accession>
    <name>A0A8S1QMZ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1890004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000198">
        <property type="protein sequence ID" value="CAD8117123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QMZ7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="124" mass="15234" checksum="40B190B0EE02AE1C" modified="2022-10-12" version="1">MEKEDLYYKKDIYQIKKYNLIHHQLLEHYKQDFNNLLFRQLVPSFLTIFKFLALAFKPSTQELKVHGNYLVMLEHYIGQQQELDQTKEDFYKQRVKDNYQFISFHDTQLDPVHLQVSTVIYKHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNG9</accession>
    <name>A0A8S1QNG9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1780003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000187">
        <property type="protein sequence ID" value="CAD8116714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNG9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001434">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="167"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="212"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="50767" checksum="CD4D8AC9B4DF9C74" modified="2022-10-12" version="1">MNKQLIVGQYENKVEECVQGFQQYLDLSFNRIDTFLDQIAFDPQPQASIPNKNINNIPKFDQIQFPSILQFTTQLIQEVQSIVKLTIHKSFQLNQQSNTKHINQHNHNQLNFKPFTYNLIQNHSIKQTEFCRAIAINQDNSIVLAACNSQIKVFEFKQEQLKQIQILTEHQNTVNTLNLMKKSTQFISGSCDKQIIIWSMDQNNQWICSQKLNEHNSQINCLLLNNNEDLIVSGSNDKTIKFWRKQNQWKCSQTITDHTNDVVGLSLNEKQNRVISCGYDKLILVIEESSQDQKWNVIQKITVEQYGYRICFINDNVFTFQPRLKDQMYYYEINNTNQQYTKTKEIVVKSDYNSCEFQFPQQYIKSKCLLVNKNGQNVNLIRKNQNGDFITQQSIQFGHSDIFGQISEDGQYLMTWDWKSKEIQIRKYNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QRE7</accession>
    <name>A0A8S1QRE7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1980004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000207">
        <property type="protein sequence ID" value="CAD8117404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QRE7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="57200" checksum="707335CCE17ACAA6" modified="2022-10-12" version="1">MYNIRGQGNFYKNQWDQHCRKCQFDWSSIFPFMLISLWTFISILMSLRSISESNQLFSQLIIAQRFKKILFKLRSIKHIIKMLINYLWIYSVIFTFNIRFSFSLFFIEQTSDTSYFIAKDLDCYISSIQSVHILYLKIFTMLILMIILFNLVIAGAYLHTLITKQKHDISIFSNTALYLYVFNYAGLIKMFSSALSKREGDVSLQYGSQIHQKWMYYFIIPGIFIIGIFIPIFIYILLMLNKNKLDKIKLRKHICYLLNEYKQERYYWELIKLFKKSIIIFIMTNFETEIVLKASLLGLGLLIYQILAIFNKPFTIQKYNSLDLQTAQICSISIFLALTKSICEQKNYIFPSILIQIFIIGCFIKLCYPFVFGIARNYFKKYQFFYLNKLHFFLNLKVPNFYFAIILGKFLEKEKLRQNKLKYNIAKLKNHLIFLSKVQLRNSRQIISPQSSRILSTKSSRIGVEKVFFNNVEEYESNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QSV5</accession>
    <name>A0A8S1QSV5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2070010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000216">
        <property type="protein sequence ID" value="CAD8117707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="MNVENTE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="43185" checksum="B97E4367CB8A4CBB" modified="2022-10-12" version="1" fragment="single">MVFYPLTQQQNLLQLKQTNPQKFIHYLKMILKEFEGERLFWYKFLKTKFLIIISNNNTEIKYVTEDGQILRIDYNKDPQETPQILNNLEQIKYLQWVGEYGKNLKKVGDWIAIWKGQKLKNVGGKYSEEGKKIGFWVELTSNYMEQSLVYEAGEYVDDQRRGKWKYLFQDYEIGGGNYNKKGIKNENWIELSKSFQSDKQVTYNGKYRNGKKIGMWVECLRQNGYQSFQVIGGGYYDNEGKELKIGSWVELIDFRRQMNDYKLMHQKKQVTFNGQYNNGYKIGRWNILSSDYSNGQSKKIGGGCYDERGLKNGKWVEFDDHSASESNITYCGEYKNGQKVGLWNLYWNDDIYTYQLSGGGCYEIKX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNA6</accession>
    <name>A0A8S1JNA6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0050368</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNA6"/>
    <dbReference type="OMA" id="GFNDYSR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000117">
        <property type="entry name" value="Monoglyceride lipase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="2">
        <location>
            <begin position="53"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="375" mass="43319" checksum="3C4DF72F066C3763" modified="2022-10-12" version="1">MNLKESESINDFFKDDTKFIGEYQMLDYKVLRGYIPGFGQDLRLYYTKLDPPNKKASICIIHGFGEHQGRFLHVADFFAKMNFVVHLIDLRGFGFSGGPRGSQSIADLQLDVEVLIRQASKDLPLFLYGHAMGALVIISLLIRNPKLKISGVICTAPTLGFPLNRNIGPFKQFLIKNFGHYLEDLVINTNVNPTSLSKNNQHIQRIFEDRLVMPFLGVGMARSIYKTLPFIFKNSVQFQFPIIIFHGKQDTQSSYENSVQFINQVGSKDKQIKLFDEGYHELQHDEEFQCIKQQLNEWIKIRLENSVPFGILSQPRLLIGIPIQKNRKKLIICLLILLIIYFLTVFIKKSKFKKVQYPFVPILILYNKLIKKQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNK6</accession>
    <name>A0A8S1JNK6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040569</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNK6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="143" mass="16618" checksum="5575B086BA79A5DF" modified="2022-10-12" version="1">MGKVELSGEDGTMNKIVLQLQFISLICINKIMSKKLQMEVCWLKQVSKNMEIMGVGMYPYYLPFSIGEQFSSVVDKQLKLHFILLLKTMNRNILAECKRQMFKQILIFPKGIDNNISLNNPVCRQDENMTSLEYIAISYKAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPE5</accession>
    <name>A0A8S1JPE5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060436</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="348" mass="39771" checksum="3197C2960699453A" modified="2022-10-12" version="1">MILSIQLVQIINIXQINNRYIAKFEELEQIKEKLAQTSQRTRNISSDRQQTNGINVTEVKSSRNSEKQSQSVEKFLNLRQQNQNVDNLINSWKQQKTHSVSLNQGTIAGSIIQESPEHESPQTKPGTYPHLILEEDEQQQFLEQVSSVQPTIQNKGIAKVPITTKSTLKDQVNNEKKKSVVYSKDANMFSNTQQQAISQQNQNVIQKKNNTAVRPQTSKVQPQPTPSQIAQKKAVQSKQINMKMSQEIIADVPQTPSQQKMKQSSLLEKTRQVKMNQASNKVQTKSIQTNQGEEKILIQQIQKVKETILKLYEQKFDHQMSHSSSNIAKNFDQIVNAIKLDFRCLEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQ73</accession>
    <name>A0A8S1JQ73_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080258</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQ73"/>
    <dbReference type="OMA" id="FQHVRMD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040458">
        <property type="entry name" value="Vid27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913:SF0">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="684" mass="80713" checksum="A64EF508D4B75637" modified="2022-10-12" version="1">MHFIKDIFNKVVGTQQPLVKQEIFEFFILGTIFKQDEAKYTKISSNSEFVFDKLSNDEFDHILIVRLNRASKSYNNVIRQKEGDFYFNISFFKSIQHTTLSDSVEEFSIDLIDIQGIKWSIRIHFQKYTKQENLKIINFKLYLNQLIWIQQNKKALPKNLNDLDNLIPTKQSLKLQIHNIVQQKQMTKTILRVDRSILSLLTTRTTQDKEIIGDVINYLLQQDIHTIKQLYVGKLYILCPKLAVIRLINPLVIMSLNIISITESRMELYNKDRQLLLIQSIKDFISFEVDKDQNYLSWVYFDGLNQIVLYFKFDILYGSTSMAIILEKFRICMNEKQNLQLETGTSFSQISTTTTINLNESYNQQIEFKKYIYKDDLHTIKGDLKQATTKKIIYNDNNNLIILEDKTISFIKNNKTYQINQFIKFDKTYLDYNSKILVLYLQYQCVLYLIDLGTYTYTQDIIKLNFKIADVCSYEKQFILLNNQSVYVLDESKSIYKINEIDQNLKDNYLIVRCSINGSIVIGTEQGIVIIFDNIKTMKQVNIFEGLGDLIQDIILSTDEKFIIINNSQYIQYQQIITNKQNGYQQNLDMKPSPIRVQLNSEDLILMGLYNYPNFQHVRMDKNNRVIAAQLENLQVIFNVQQIINHQNNSYQIFILPEEIFDQQYREQDLIILSESNIYIRECL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JR52</accession>
    <name>A0A8S1JR52_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytosol aminopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0090108</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JR52"/>
    <dbReference type="OMA" id="SSMEQMY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF23">
        <property type="entry name" value="CYTOSOL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="5">
        <location>
            <begin position="353"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8045083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="522" mass="58495" checksum="CBECB834F71B3772" modified="2022-10-12" version="1">MQKHTLMETTPHIEIKHTTQAINKGVYVLYYPSINESADPIVKALQFYFKSKELLKDINSGKSAKIYALNDTDPFEYVIIQNATSPRMDGANLARKASNKIANQKSEDNSISVLLPHNYTVNDIAEFSFGFQLANYRWTYQSIKEGGKSSSFPINYLNLITSITQEEYLNQVEQHWNLKYLHHLVKPILRLRELVPTRPNIATTGFMKNYILDIVKGFGDKVTSTLIEGDELLGVGLRLIHAVGRGSIHKPFYSCLSYKGNAESDKYYALVGKGVVFDSGGLSIKTTSSMEQMYDDKGGACTVLETFRAVVELGLPINVVCSTAWVENSVGPDSYRVSDVIQSYKGITVEILNTDAEGRLILADAMSYAQDKFQIQEMIELSTLTGIIKNALGRYCGVFTNRKGCFSLLRKVEKITKEPFWALPLEDQHRELIKGSVADINNSSSGRVGASAAAAFLECFVEKKVRWMHWDIANVAVTDKNEGIYTKGGTGFGVMSLIYYMTWDIVSKEIKEQKQENEDDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JS64</accession>
    <name>A0A8S1JS64_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JS64"/>
    <dbReference type="OMA" id="HTKSMIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="221" mass="25699" checksum="9D5DBB17DD0CF6FD" modified="2022-10-12" version="1">MGNNCMIQNQTINQNEKVIRIDSYSNLQRPSKQILHPQIQNLSQNLDTFEEDFFKNEFLFDVTNDNITHSYDSKIKLEKTDIDDIQQNITKQCPSLNFVGEQQIQYKINLNTIQEDPLNQAFIKKKTKKDGNSSKKSQKQQNKPVQKKLEPKGILKQNKQNSSLSSFSDRSNKHSVSFNLLLGQKDNKNTRSMSPIIQTKNKIKPNCHTKSMIFMQQFPYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JUW8</accession>
    <name>A0A8S1JUW8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">33 kDa inner dynein arm light chain, axonemal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0110033</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the inner dynein arm light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JUW8"/>
    <dbReference type="OMA" id="RQMAVNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183:SF0">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT INTERMEDIATE POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183">
        <property type="entry name" value="AXONEMAL INNER ARM DYNEIN LIGHT CHAIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8046654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="28342" checksum="212ECBB8BAB5BDF9" modified="2022-10-12" version="1">MSDKPNIPAPNSLVKYASATLVSTSGKPIKDKKKGRDAAPQSITNAQTEDILNSILPPREYTMEKQQLWIQCVSSTPAKREDVILLQENLDKKLQQRQARETGICPIREELYAQCFDELIRQITINCAERGLLLVRVRDEIRQTIQAYQTLYESSIAFGMRKALQAEQRKTDYNNKIKQLETECQDLSKQVEKIESTIEDMQRQDQEQQEHEEAKHRDQVNFLKNANKVYKEELEKFLTGANVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVN7</accession>
    <name>A0A8S1JVN7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0060162</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0110165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVN7"/>
    <dbReference type="OMA" id="EVESAYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8046914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="127" mass="15059" checksum="56E5B19F53012199" modified="2022-10-12" version="1">MIYDQPEHFGLFEKTVTAKEKEEVKQFLKYDRHVPLKLTEQVFHAEADASRFERLGQVGGAVYSFSFFFFPIIKGLPIKQRLFWAAVPGGIVAWLGWRIKEELEWNRVYNCYQKYQVAHSMHKKVFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXW2</accession>
    <name>A0A8S1JXW2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXW2"/>
    <dbReference type="OMA" id="CTNSKPP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="65" mass="7421" checksum="03A570FD82A3AF9E" modified="2022-10-12" version="1">MGCTNSKPPSKSQPPQSARTQIQIQFQSQEESPYVIKKNPIFQRRASQKSIISPLQTTSYVQRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JY74</accession>
    <name>A0A8S1JY74_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0120236</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JY74"/>
    <dbReference type="OMA" id="SEMDIWR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001577">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF6">
        <property type="entry name" value="SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="14"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="307"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="380"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="569"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="380"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="402"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="413"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="569"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="582"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="650"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8047926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="744" mass="86988" checksum="39027D47FF62EDC8" modified="2022-10-12" version="1">MNVQSTHQSTLQDFQVIQELGSGSFSNVYRVKRIADGQEYALKKVKIANLKQKEKQNALNEVRLLYSINHKHIVAYKEAFIDEPSQCLCIVMELLSGGDVYKKISQARGSTPFSEMDIWRALIHITLGLKALHDQKVVHRDLKSANVFLSNDGTFKLGDLNVSKVAKGGFVYTQTGTPYYASPEVWRDQPYDMKSDIWSLGCVIYEMCCLQTPFRAKDMDLLFQKVQRGTYDKIQSHFSKDLSQIISSLLQINPNQRPTCDQILANPIVQKHMKNLETNIETKPINKTLIETIQFPNNFKQLKNQLPKSNYDEVNPNVSAERVQDNKSTDKSTSPFRYSQQQQYIPINESRISQSQVNVIQTNQQSPQQIKNSSILEKNQSVVQQSQQNLRQQSLNNKTEKPQSAQINQRQNPYERAHELREQREREIKQQREKDQLLREKRERDARDAKLAKEAKEAKEAKEKQQQESKLRESREQQRLLMEKQQREQQQQQQQQQQFRRVSPISAQPRDYSYANYINQQQPRSQHSYSPITRSMDQQNIQLGQNQRANYNNQIQQRPIISQSINVNQNYPNQQNRPYNQETSQHRPSQTPQSAAPRQQYSPHQKPITDRYAVPHPQSALPSSNVREIQKTERPQVFPTQAKVIKQRQSDYNQKNDFTSPNQQAIRPAGKPSRPQSNNIFSQNDRIVKTDSRPQNNFITGNYNHYYPIDNYQRHASDQNLIRYQLRQQDRQVLLRPQINNRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K056</accession>
    <name>A0A8S1K056_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nephrocystin-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0100328</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="IDEKMEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058686">
        <property type="entry name" value="Ig_NPHP4_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029775">
        <property type="entry name" value="NPHP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058765">
        <property type="entry name" value="NPHP4_C2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31043">
        <property type="entry name" value="NEPHROCYSTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31043:SF3">
        <property type="entry name" value="NEPHROCYSTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26015">
        <property type="entry name" value="Ig_NPH4_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26186">
        <property type="entry name" value="NPHP4_C2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NPHP4 C2-like" evidence="3">
        <location>
            <begin position="694"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="NPHP4 Ig-like" evidence="2">
        <location>
            <begin position="1130"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="485"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8046390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1309" mass="151979" checksum="3CB0E94CCA5C3DDC" modified="2022-10-12" version="1">MSTEDINSIKKFLKFQKMHKSLPSSIPIQMGLDSKAFKLIVSRIGGLRRPQNPVLNLQFSVAANFFIQNQQGEKCFFGRTYNSPRLNVEFGPDGVNYEHPIIPIYYHTRILNNKEMPLLIILEIQSYETQQDLVLKRMHLGWCELQALTITTQSTKLNFRKGSPRILLLGQQVNFESSGISVTCDLVQSEEFQISMHLSPENALCGLGDELPGLKKSFVGGDFELQRSMVVFMSKIQVLIPDTMEADIYRQVQKYRTYKYRTPDDYKGDIQIINKRLIVAFHNGWTFINTRGTNNFVPLGETRMLVNEFVDQKQIQYKQLAYHGVIDINNVCSTDPTVNSGMLVAQLEYEAMFLVSNLTKETMQLTLGWIPIYINNKFGADELVEEDFIVGPGKTLDGRPLVSFQNPELSIKLKGSLNLSGGGFQIQDLYMGAASTQQQQFPQQQQGLQQQPNLNLNQPHQQQIQNKQEQQVRGSDIDIRTQVKLKDFEQQLLRLQEENERLRRLAIQQTSDPQKQQEYLQAIEKLREENRLLQFTQARQFEGLEKLIKDQKAVDRTAAPPVVLAEAKPQNTVIKPIDSYAYPVPSATLKPITRTDVTALIDDPISMDQENRLMALEMQDERKDQIIVIQPVSMKQILGPYPICKSVQIGIIFYDLQTFKSPFLNLEYYNDERNFKYNLENGNPLRLLYNNEVFSCKFNIETSLQRYANLEQDFARYLFNKNLILDIWDSESLFLFGSIRIPLRGIMRGGKQNAQIMADVEIQDPQSKKVKGTMQIVIKNIGKIGQQDMSKTVPLENTQNGFQKKKLVSEKPVNRMEVPAQAFDGNSIQDRIKKIKQSNLQEMIKNKQSQQRSLNEVLKYKEIKKPSLVRTIMQGFLQNEKVVHSFYGKCEIVPLYFKNTRPKPEQFTVNIEDPESRMLEYQEFFLIGNPAEWKYWVTNNKYPEPENYDLIRMKENQGKTQYYFILDPNQEVTLLFKFISFRQYDYRVDDHERTPEYIKKYMQSRHITILLQQDGKMMDGLRIIVEPHQQPIDHVLRFYERDNRQTRLVIPRLFNQPLQQQPHVHITDPDVMIEWSSDFNSIRLNLRIGEPQSVKIFNILLYSDQYQHKIIANWKIEVYSLSTIDIEVPLGQRVCTKITFPCDITRIIKMSSSNKMISFVKPFDSEITMLPGKINLVPINILALQDGKHLIKLNGVDIHSQELIYSWLVNVHAEPQPPSATYDLRCIDGRESRHQVNYKNQVNYTTQYNIVSSSEYVKIIDHSISTGPNEIVQFKIRVLPGSEELVRIFITDIDERIFDVIVLNIKYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3B6</accession>
    <name>A0A8S1K3B6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0080387</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="QDYQLSI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051197">
        <property type="entry name" value="ECM-binding_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33150">
        <property type="entry name" value="EXTRACELLULAR MATRIX-BINDING PROTEIN EBH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33150:SF1">
        <property type="entry name" value="EXTRACELLULAR MATRIX-BINDING PROTEIN EBH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="652"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="720"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="865"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1379"/>
            <end position="2093"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2120"/>
            <end position="3018"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3047"/>
            <end position="3198"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3407"/>
            <end position="3861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="655"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="690"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="723"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="760"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="782"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="805"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3872" mass="462338" checksum="1C2F9F2089292264" modified="2022-10-12" version="1">MLPPQNNTVEAQANLFENEQNQQLQQCFELKMNIILMATEIERLHKILVNKKDYYEKKIQDLIDRELQDKEQIAKQEQAITELNQQILQKNDFITQILQEKSQQEMNLLISQQLFVINTQYKNAEIYIQELQKENQLLKQQFEDAPSLRQKLYDLEQWYAKLIQEKNEEINLLKTHQPHIIQHAPQLNIINQILDQKERELLQWQYSSQYVNLYSENQKLNQELKFLNDKLNLLLFDNEHLNKRLADIQSQPSNQTTQPQYATLRGESQCTAPNLYDYQNTKTPRRYQQQYQSIIPTDFTNTSLLSYRIIKRPQSVGSGSSLHFLGQAPQIGYPQYQHSPRYSRKSLQNNSAQFNEILRQIQIIQNQPIITIQNIETQIQFLNHLLLIKRQELPTQTTPPRQIYKILKTQENERTSSPNYSPSQQIIIKQPINYLQQSTQQKLIPQTVRLLPHNQLQYPNNIIKYRSFSAETKRELSPNTVQYQQQLIPQTSQLFYQSQQVHQIPYKCSPIQTSIPQSNLLNTIPKQIQRSFSPQTVQYMPYQTQPQAPQTIRFQDLKQYHSVNHQQYQDPQQQQNLVNQNFISQSYPSLPTQYGAYSQNPNDQNKSPIHILAYPYDYSPQKPEDQNKTRQLQQPISQHPNELFMTPNTVRKPVISSSNSSLPFSQVRNPLKREQQEPLSIRKQKEPKHQSNNSLPAQIQQSAPIDSSQVEPFELCNAPQEPEDQKKEPEEQFKQPVKPEEIKKSPQLQDPAEQTKYKTPPQQQPNDQPKQDEQFQQPQTPNDQCKNTQNPDNLYKQPQKPQIHLNKQQDPNSLNKQQINSQLQSRQPIPQNLENQKNELSQPQQISRNQPTSIRKSQENQEFALLQDTEELRKYQQELERLNNLLNQAKLENEKLKQLFQQNDLNWQQQFQQKYTTALNQLQLFENKLTIQNREYNQNIQKQQNNFNQLQQKYDEQKLVIEKQLNEINQLKQNQNQKSKKVVQNEVQRDDLQKQLIDLQKQYADLQTKFKTQNDDKLQNLNKIAQQGESISNYEKQLKQQTSKYSELNADYIQLKQKFDQLQKESTSSKLSQSKEINQYQQQITLLQQQINENTKQLKDKVNSLQSQLLLEQEQHLILKKQYENFQRDFSIQKSQNIEIQNSNNTLRQQLIEKEQKLKEIQIILNSKTQVIQQLEIQIKDAQVQNLTTSQQTQSLNQIQVENLKKQLQSEQQRVNELNDQVSKLQQQIFSQEQQLQVRKQTNIKVTEETQNTKNIIQSDYKQEKQQLQDENRDLLQKIKKLEQILQMERENYNQSQQQIKDIQNKYQTNITKSSQENDEQLKNLKSQIEQLKSQNQNLSNQIVDMKSNYEMQISTINTKTSSKIITLEKQLFFEKEQKENSSTRILQLEKQLADFQNIIKSDQTEELRGKINQLENQILKLQKERDTLLSSKQRGDEEIKILNIKISELSNKNNQNELELSNLRSKQNIYIESTSNLTTLNQQILILQQEKQSQINEITLMRQQIEKNKQDHEFQLAKIRSDFNKDLESQQDINRKYQNEILNIQNVLQCEKRDREFVQKCKVQADEMILQLNQEKQMLQDQIIKQKSQIEQLQLDINKLRLNQKNSNDEQNLLLQQQIQNYNNQIDNQKINIQKLNKEIERLNLQVQQMNVIRADLESENFNLKQQIQQVKQKNQTILQENENIVIELRSVANNSEDQIQDLTLKLEQQIKETEKYQKDAFNNDQLAQSLQKKLSDVNLQNQKIKQQYEMQIQELIKKINEKERLDQQTLQKTQAFPQIQNTHLIQENEELLMKIGQYEQMIKSLQEEFYKQQSELQKITKEYNTIINQSEKTVVTITQYQLQQDNIKRKEDQIKQLQQQIQDYQKKLDSERNSYQDENNRLAVKNQELSNQLQNNLNKQIIGENFNLKNQIQQLQQELKNTPIKETIIYQSASPSKKNEEENAKYKQQINQLQFRMNQMASDYNLLEQQTEMEKLQLEKQIKNLQEYQQKLQNENNQQQRQIEIFQRQIQQLQMNRSNDLCEELEFEIKKLKSQLEISQLQCKQIQEELISAKHQIKQYRDDLVDEQNQKNQLNRQIIQQEQILSELKKQNNAQSSIISETTIIKEQKQDQLLQQRINQLNTDNISLKNKLQNLEDEYMQKFRLQQLKIQELTNSLNEYKEESIHKVNILEETTLNSISNDQTSEIRKLRDEKLFLQQELQKLQILHKKQIEEIINDYEFRIEQLKFTIIDLKEKKQVETSNDKNLLKENQMLADQVKNQQNQLDLARQQILDYQQIINQYTNESNKLQLLQKKNDELTYQLERSQKMQTNNEQSFLKDKQLLNDQIRQLQNEIAIVKNNEQSLIKDKQLLNDQIRQLQNELSIVRTQIQDYQLSITQNTNESNKLQLLQKKNDELTYQLERSQKMQTNNEQSFLKDKQLLNDQIRQLQNEIAIVKNNEQIYLKDKQLLNDQIRQLQNELSISRTQIQDYQLSITQNTNESNKLQLLQKKNDELTYQLERSQKMQTNNEQSFLKDKQLLNDQIRQLQNEIAIVKNNEQIYLKDKQLLNDQIRQLQNELSISRTQIQDYQLSITQNTNESNKLQLLQKKNDELTYQLERSQKMQTNNEQSFLKDKQLLNDQIRQLQNEIAIVKNNEQIYLKDKQLLNDQIRQLQNELSISRTQIQDYQLSITQNTNESNKLQLLQKKNDELTYQLERSQKMQTNNEQSFLKDKQLLNDQIRQLQNEIAIVKNNEQIYLKDKQLLNDQIRQLQNELSISRTQIQDYQLSITQNTNESNKLQLLQKKNDELTYQLERSQKMQTNNEQSFLKDKQLLNDQIRQLQNEIAIVKNNEQIYLKDKQLLNDQIRQLQNELSISRTQIQDYQLSITQNTNESNKLQLLQKKNDELTYQLERSQKMQTNNEQSFLKDKQLLNDQIRQLQNEIAIVKNNEQIYLKDKQLLNDQIRQLQNELSISRTQIQDYQLSITQNTNESNKLQLLQQKYNELLQQQEKSSHLITQYKQQLLNIQSNYDNKDQELNSLKFKFQNLYNQQNESSLKLKNDQEYISKIQENLDQNTQQTKQLKEENNRLQTIILEKVKEIQSLNQITQTLERQNQDINLQNVKLSQQLNDQEKICCQLKESLKYTSLDQNKGLQQQYNILQYENQQLDTENKKINNNLLKFQQDIMNQGNEKQKLLEHYQQETSALQNQIKRLKQQILNQHEIQIQQEQISKIEINQSSQNMNSPKVKNISKSLNKSNASCKSTDDLRGKYYNCSLYTTNNNGQNYYASLRAVKSKGDLLISHEKSQSTSEEDLKKQINLWQQKYEDLLRERYSKPYMITSKQDRLIQEVAILDESVNWKLKYDELQEQISKSKQNTNEKIIQQLRLKNELLCSELYQIQTDYEQLKTEITINQKQQTSNMNFNNQNQQKIIDQQNQKIHNLQNEIDQLRQKLKQLVESQNNQEKKFNNQQNEYIQQLETDNYDYQQEIQSLQIEIKRLQENKNLIKQQINDQVINLQSGQDKNKINKEEYLQQIEVLKNEIGIQQNQIFQLQNQLKQSKIKESELEQKFKFAIEDLEHVQQMYNEQRQSCQEQQRQLQNCQQQLQQQIQKQQLSQQQQQQVQQQQQYSQRQQLQEQSRIIETQAEQQSNNQFEDQYRKILNELSLIKQQNLSLQTQLQESTQKQITLMQEIKQLQIQNERLNLSNVRLHNEQQKVTTQSDQVNRINEQLKELQFENQKLRSEIIYLQQEKQQIITSFESKLQQYSASANNKIRQQQIEDLKRELALLKRATLKSSGIDIDAERLSYTTQITQLKKQLEYEQIKNKELQQKLQNSQQYQSDFGLVERLNKELQDKIVELHQVKSEYRNAIKMIHSLEEQIIERMEQEQYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K410</accession>
    <name>A0A8S1K410_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0110217</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K410"/>
    <dbReference type="OMA" id="IKSQLKC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8047016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="362" mass="43337" checksum="1AA273D6C31D283C" modified="2022-10-12" version="1">MHHNIVVSQEIRPNKVYLVTYPDGSQRYVKKYLAQQYQNIFDVLNTLKQQKFRNIVKIFDVIIENNELICVYEEACKQNLREIIKSNEKTFDTQDILKCLLDIIGGLLELKNLNIFHRDIKPDNIVYNGQDYKIIDFETAKRFQDDRTKVQSVDIGTINYRAPEINQNRAYSSQCDVWSLGCLFYYMLFKQEGDHMQQLSIPQNHYYDDKIIKILKSMLEKNETKRIRLDNLQKEVQELHNIYNNPTQSTGLEQNTTNLVSDINTTIDKRHNNINTFNNIMTKLIFKEKNEQKIIKLYQFWTKRLETKFSEEIQQRQKIEKLLLMQIQEFKQKRIQIDVFASIDQIKNELNCIQDLKDFYDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K526</accession>
    <name>A0A8S1K526_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0110418</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K526"/>
    <dbReference type="OMA" id="RGDNQHT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="213" mass="24508" checksum="849F6A384FD9E02A" modified="2022-10-12" version="1">MDSLFALFDQFFRINLTQYPQNQQAYLSESWEQFLCLLQTQPLDYGKIYTLINRINYEDMKVEKNERQSSSQRYIARNITAASSPRIVSSSVQKNSTNVLSKIAQVDIVASPKITVSNLTSQQSLTNKTSLQQNVKQQQMIDEFNRNLLLSKIKELQFNFSVSTDKLYAIARVSHSRGDNQHTSKSPIKYPLSQYINKSRGSNHSAEHYKIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K5Q5</accession>
    <name>A0A8S1K5Q5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K5Q5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="207"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="843" mass="99920" checksum="B3B7772A4322A0D5" modified="2022-10-12" version="1">MPSAIQYFSDLDSLHHVSNNFLRPQSAVSLYQNKKISSSTMVDNKQVSDTRLTDPRQTPRYIKKDKEELYQELLQLQQKYNQCYDENTKLKTQISSLEKQVIHLTDLVRNVEMFCNKKTSHFNVFQLKKQIQDLKSNLKTKDIELESLKQNVKTTKIDEIRIELQESHIECLRLRTMLDQQLRQQVLFNYNDHTLIEEKLYIQSQMMNQLKTENDQMTGLLKIQEEETYYYKNLLAETLKTVRRYEETIEDLHKNLKGKNQFMDKLAQKIEALKNTNNGLADKVVKVEDLKEEIRILKCTIEDQNKQIKSVLSDNEYLKGMVHELKIKNNEKSEIQAKEKKVLQDQITKISNQYDLLDDKYKNLLLIQVQAKNKETAQSSMIHKKEQTIGLTLKNNNDSFQQQQIYVPKKLRAIKMNDVLHIGEELNYRFRIKKIILIDVIEQYLFDDDNKKEKKVSIKQLQKRFEKEPFMLFEQDKALMFARFLIEDNSQEFVEFNIELTETLDRVKSIFTKIIGNYRIFTLDEEIKHKEDITKLIIKYKNSLKQHFDSLQSTNGELTKRQILETFIFMDIDINNSQLEYFFLKLFVFSNKIDRFPYQKIFEIFQQIQTTSTPQLQRKETQRKKATTESNKQPVYVTKSLFKPNVVHIKIIKDAILSDPWVCILNAIYLCENCAMILSTEAGVTNIKKYSQLNAEEKEKMQKGGNVKFKLNVSNVNDKFHSHKALQYRFELALGLSEYDSDISTNYASPNKSIIDYPEQKKRLTKALELEGMQQDIEWLQIQYQILTRDLKKKIDRNFYLTKLEEIGKKFVEKQQQKQVQQKQIHNQNDVFSSFTALMKKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8H3</accession>
    <name>A0A8S1K8H3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0180207</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8H3"/>
    <dbReference type="OMA" id="QMAPEIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000106">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000034">
        <property type="entry name" value="Kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000476">
        <property type="entry name" value="PAS domain-containing protein tyrosine kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF298">
        <property type="entry name" value="MIXED LINEAGE KINASE DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM" evidence="15">
        <location>
            <begin position="221"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="455"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="341"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="349"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8051589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="728" mass="85037" checksum="3F709F811D3C6867" modified="2022-10-12" version="1">MKLFSFLKRKTNNLHHITYSNNDEYHGEMKDNLKHGKGVYKFDNGNRYEGEWQNNQKHGTGKYYYNSGELYIGQWKQNKKSGHGQHFGQYGDRYVGQWLNNYKNGKGTIFYGDNSIYSGQFYQNKKNGPGYYYSSITKELRFQIFENDNLLEQNPVEKIPSEFENVFSAYLVHDNHIKHLQSLQNKSDENNLNQQEVHGFETLQALNETVIVKGNKKMQEWNKDQVCSWLECLGLSQYKENFMKNHILGETLHNLTDRELKEELGMQILGHRKQILQQINMHKKYYIKTMSNQVQKTECDNSADQSSKYDQLFSIIQKIEPTIEKSETKISKQKKLKQKITQETEEKQFSPQSQKKQSPKQGNQGQSLKTENNCQVSNFNIDNMNKSVSLEENSFISIENQQTQLIKENDYQKCLEKGYYNVQEINLQQKHQEKIMCLLKDLGINEKLLINFQEIKQGSKIGEGNNGIVFKGNWLGQDVAIKSYCQKQDQSQNKQTMADFLKEVQVISDLRHPNIVLYMGVCIKRHNFYLITEYMENGSLYDYIHKRKTKNLNFVHIIEDIALGMNNLHGRGIMHCDLKSSNVLIDSDWNVKLCDFGLSRIKTKKTKSIIETSYQMAPEIMRGEPYTEKSDIFSFGMILWEIMTSKIPYENLSITQMIETVGWGNNLVEIPQKSNPPILAILAKDCLQKEPSKRPNFARIVERIQQSKNKKCHNKDKAKKLLIDFLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K9Z8</accession>
    <name>A0A8S1K9Z8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0180111</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K9Z8"/>
    <dbReference type="OMA" id="PIREAPR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06612">
        <property type="entry name" value="STKc_MST1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001091">
        <property type="entry name" value="STE family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF2">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="11"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8051467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="556" mass="64167" checksum="5A820E3F29649009" modified="2022-10-12" version="1">MYSNQDPEEQFELNELIGEGAYAKVYKGKHKNGQIVAIKIVPSTGEIQSLIKEIQILKECQHAHIVQYYGSFYKDGNLWLVMEYCVGGSIIDLLKITQKTLTESEIAAILYHVLLGIEYLHANKKIHRDIKAGNILLDEKGTIKIADFGVAAQLIYTNADKGTVIGTPVYMSPEVISKNRYNHLTDIWSLGVTAIELAEGKPPYSHIHPVRAMFAIKNNPPQGLTNPEKWSKEFNDFVKSCLKVQVSERPTAQQLLQSPFIKQGKQDQKKLVNLIDNFLNQIDEYRQSKTQGNVEKIQTLISKQEDSQDEEQSCGTVVECGTLVIKEEEAQSKSKSEKELDFVTYAKKEKIINEIIDEQLSDEQQMENMNQDERKKFLAFLQSQMEEEISKVKQKYMPKIDMLTQKIKQNESPELPKLQEYIPQSKQQEYIPQNRQQEYIKQQEYISQSKQQEYILQSKQQEYIPQSKQQEYIPQNKPQQLIQTQQSQLIQQQQPQKIEVSVPFQVNPEVLQKLSNMKIPQIKIQNVSSPTAQLRSNPKQKVKPEYLVKQNINSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAQ4</accession>
    <name>A0A8S1KAQ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0170201</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="208" mass="24016" checksum="ED34FD6B5DD59C92" modified="2022-10-12" version="1">MEITTLKDLIIKQMLDLQSTVYCVIPKLPSIKSSNYQDGILIGRILLQANEQIQNIVQRLIEDQNSMITRSKFKGSNINNNLDQNPPKQIQRELHQNKEKILQIKITPEPNIDRPCCLCYEGNENTCGKIITIPNSVRKLQAHSQCFKNINNPKQIEFDKVYLLYRTYECTICEKQGASFVCEKCNQKYHFPCFVDSSQEGILQCNCX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB44</accession>
    <name>A0A8S1KB44_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB44"/>
    <dbReference type="OMA" id="MQIKPQL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="391" mass="45572" checksum="A382921B0A9C785A" modified="2022-10-12" version="1">MDDIYSQMTDKFNCDSSNRTDDYFMQVCQPLYSNRCQQREEIEFKFRQSKQDYVFPRIPFDETPDKYQTIESPHRLKTEGNEKSEDQLDLNFNFNSSSEHLSEDAVVLQPKPYQQKLKPPQPKKSKHPATQQTYRTILNDLERKLHTINCKDFVKKKCEQKGASRAKSNSNSTTHYNSHYQRKPFEIDTKTSSVKKSIPTESKKSLSKTNLAAILSKQKSKSNPSKANNIFNVNNSHINLNVNINSQTMDLQQQALTVAQILQKKQLLNRSSLSSYQRNSNRCSTEPMEEMKARLSNNNNQKNKTTVTNQSRCNTGMDVRAQSTIQYLNQMKQQQNKKHQKQQQQQFQQKQQPTMQIKPQLNIKQRSSTNTSKPRISTGKYKKYNLSIEMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCU8</accession>
    <name>A0A8S1KCU8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCU8"/>
    <dbReference type="OMA" id="IENAQMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="77" mass="9082" checksum="7710FDD08EB5CD4D" modified="2022-10-12" version="1">MKPLSTRKQINNKNQEKMKLMVTKKKISKKSGPRITKTIKETIENAQMIQPSAGTRRLPRQNRRNKLARQNALRRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KF52</accession>
    <name>A0A8S1KF52_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0190161</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YCENTAF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010032">
        <property type="term" value="P:meiotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026971">
        <property type="entry name" value="CND1/NCAPD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032682">
        <property type="entry name" value="Cnd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222">
        <property type="entry name" value="CONDENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222:SF2">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12717">
        <property type="entry name" value="Cnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="7">
        <location>
            <begin position="231"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1536"/>
            <end position="1556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8052395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1575" mass="186397" checksum="621FEDA4DEDA08F6" modified="2022-10-12" version="1">MGNCYDSRKNTVILAFDQAEYTQLNSQFQYYCELSSETRDIKQLNQRSFEDIFSENPTFGIKLFKFLELYSGSEGYVKKEPLFEFLELLVKDVQTTVPVFKNLERFELMSLITLQNYKYVKTKEELAQLQLTYLDTLNVIKDIIKMYQHTQKNTPTNEKYIKSLIDQLYKNESGSLSWIQLMDFISKQMPGAKYLIKKYFQAKFMGKQFNVYIIQQIKNQNTIPVINTPSYFVTDELFFQLLLSTQSVLTDCNQLTLLYSNVAHQGGFNQMVQSLKDCKLPTIMFFQHEEKYEQKIKQQIFGALTNLRWYDTKQYFGTKKDCIFSLYPYFKIYRSKKRGEQNYCFLDSNKGIGFGGKNGEGYRIWIDKNLQNSYCTQTDDSYENGPLILPYVKKLQINIIEIWAIQHPTDENDENFDIKIDLKDPLLQKQDDIQFQNSNADFYWVQQQKTDQRHSGYYWENSNLSEYPSIQFDNSLYNSLKTKDSRLILRQMRSAIEEFNQNKHFDSETFTILYYGIQQLQKDDILEFLEKEVLKRQDVLEFEILQMKFLQATLDDQNKNRKEYLYQLQKEILISFDQYDKHGQMGIETILQFYKTKIKENDIKQYLQNQAKKIIRKENLHNYIKNRLIQIVFNSEDCDQQISDLLIYCENTAFVTQFITILAQHSLEILQNESNSLKQVSKVFERVSAQLPNLFNQNLPVFLQFYDSECYCLRNAINTIITNILTEHLNPSKVEQDIEMKENQLNQRLFLIQKLSSRIIDKNAYARVHTLKMLRVVCSQNLIPPNVQLMMFPLVIARVQDVSSMVRKAALAFVKQITKFMKNLFVDGLQMQNFMKITDIQEQNQYLGNEIHKTLIAIDQILEDKANAQTEAEFKELEDEENLVVSNLREKKKLQSVFIQYQEFMELLQKLLPNIELLLNSKCITDIYKAISLISFLFKQKIENTDVGIMKMLIHSNNPQIVNKLAKKFYMLFIENDNEQVPINNLIHLLKISSSKEQIYLEELIRYMIKQKMIQESTIEIVWTNFIRNYTANQHEQLNQYSKLVRVTFGQQSNLLTRMKLDQISKIIEGQKKEVNWITVIELAKLIYYLEDKVLVKKYFQQFSLILYFYFGTPNNLWFVACQEILNLIQFLPVPESIYDYIIKKFSFKVFQMGMTPKRSTQDFLEEESTLVFNNQCIQLSQLLFIVGQSALAIILHIDEIENQLTHLRNVEEAKENELDKIQGGCEEEYEYNCVYLQTLCKYSVIQKGFYQNFAPIVLEILTEIEEENVQLSILHKSAGLCLCKFMCLSEQFCEEHIEKVFKLIQNPNSDGIFKNNLIVAIGDLLHRWPNTVTSYCRQLFDGLNNQDFIVKKTSLNMLSHLILNDMIKIKREITDIAMLLTDPDENIQQSAKRFFFQIQKKDSRRILNSLPDIIFSMSNEESNDKYKMFLMNTLKYLDKQTEQLVEKLLRRLKENINEFEVQNIVFTLSKLQMKESLARKFYECYPYYQERLENPQIKEYFQIIIQKLMKCGLNQENKQILLEFEELLNRGETKRRVLGSKNKSQKSTRSHTNSQRSIGYTNILDQLYTQSTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHM7</accession>
    <name>A0A8S1KHM7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0220158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ALWIFIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1059"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1102"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1238"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1343"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1572"/>
            <end position="1591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1635" mass="192835" checksum="0202B80FA8B98C87" modified="2022-10-12" version="1">MFEKIPAFLQKLVENYLDFFTVACYQRNQLALQLFRIAVIINGLQELQLMEDNLHQSDTKLFYSSLKESLPTTQLIGYESNSTISILLITHIIINLLIFVNIFFIKKQSYFHTVTLSVILVENYILLYPTLKAIFYFNKLQADYEIYLISIFNLIIMYIQITITQYSWRQTKLIDLQGLIRTNNFKLELLSHLILILAIGLNELSQLGSYILIMFKQIIKLGISFDSYNQNSQFMLYQNVTQIIFMTALSFNINMIYTLLILGFLIQLIHKVQSHFFENHLIQFEISLKKCQIYELLFNQSEKTSKILLLQSLLQKSFSQNYKSKTKKKQSTFLFTNQQDILQQLISQSHFKKKMLIQILCNYDLQNKPQKTLIQFMKYYKICNIYDKAYAKALQQQLNYIINQNQAYFNSRIQSINVMDTQTTVQALINSENAINFILKVVQSKIALLESLRKGFQQQIEILDQVDDYTNNIFKCRNFILQCYELKKYKLKGCQSTDILSLRILQMYFGIILNDIKESKVIEKQIDDLIKADKILQDTTINNQILLQNRYMVLNTSLINQRGKLINCNKQQISQFFGCSQEYIQNYQYIHEFMPDFIAQVHDELIDQFIDNGYTQLMKTGKLTFIQDPQNYLMPIYIHLYNPNIEDDFTLYSILTTQFQQQDYVIFDSDGKIQGISKRFFEYTKIQKSSQQDINIFDIVHRGSSILYYIPNVINLIKDLKTNIDHNVNNSLNNVRSYWQYPDNHLECLSQTQSIINYSQRMEFQPNLILGQNIKLLDQAFQQCFINTIKKKQNNLDQAENHIELLHNFQLYVIRDHIFFLITIREYKLADFSKTYTSNSMHQAQNPFTSFSTHFTQLDQLMSEKQLTTDNGTIQDIKQFCTFQHSQIIEKLFKDDDMSGRFVVDITQKQQLNASHSQSKLPFLTSRQPLSNRQLLQTGLASMRMVQPQFHLPSIDSKKKIEDYQLYELEEDMKEIAVMESIKQIQVIQNEQEIANQKQENINFNRYDSNINDDSIKMSEKQNDTQVQALVQSQIQQGSQLQMIQEIVNFNGSMKMVNIGIILNLFLALWIFIMLFINGYLINNEIQTYFHQLTNSIAYQSLQFYIGVPYTIILQLYMNDNQILDFSPALLQNTISKIPFLYGFSQMQQSEFQDTVCNQPKYYADYFANSNDCSNYFIQFHTAILQSYLQYQIDETFDYQLFNNNSFHRNQSFYGQKILLDSLTNFISSFQKVTLKDTFLILLMINLLVVTIIYVSQFQMTKSKSNLKQKFYQILNRLSFDEIVELIARFSCFKVALGENAWKLINYYDMINYNNIAKEKSNLNAHKKYIQLSDSKLKKKTDLFQIIIIVSSFCCWILFFVLGFLLFYFLFQEFAPSFRIAQNFVAFKQKLDISLCIGFWIKTDPLTNMTRNIQEQQQMINLFMNLTDSLQPLLDEISTDLVKSTYLNEEKMQIIQHTLNSDLCPSYQYLFCYPDKLSTQRYYDSESYASILSGGITGFVAQIYKFSVSEFTIEKETLQYSPYVDELQLLVQEQVFKNIFVQYIQDIQFTMYNLFAQFNDGNSYAGQILLSYMNYYSFGFGISLIIVYNIIDFIRIRQSVKELDQLKFILLVLPEQKFENQIIKQKISEISKYFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KI83</accession>
    <name>A0A8S1KI83_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KI83"/>
    <dbReference type="OMA" id="IMETGRN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="470" mass="57450" checksum="EA8853DB479C7EF6" modified="2022-10-12" version="1">MNKQLDFNPKFPQHNPFKFLYYNEVKNGFYLDNPNELYIFDEAEGWADQNGCYYQKDGAPAGWVFVSQGKFTRYDMNQRKVETGKNIYYPETIFDEEMQNEGKIQQQDQKSQKTSNQDNKNPSQYQKQNDQCQNIKSNQSSATLKEHQQHFQRFVQENNMQLHQIYFKKKNYIKGYQMDQQSGRQRVKSEDYRRDQRYIYNQNMNEFKSYNKLPQKNHSVYGKYQNINQNGRQNHHQHYSNETFKNSDHRIRSNYSSKSNYNNNYESNSRNFNNNNHSNKRNWNKSNENDRRDYQQYQNQKQYNLVKNPFIDQPLRQQYYQNQQNNYHDRRNEHNQIEYNIQSERIKIQKSNYDYQKNQNNNHSDYSNYQNKNLNVNKDGYNYRQNNNKYSKQNENNKIIQQLNLQNGNFLDQKQQYQYVLRKDNSYSQFESNQQPIFEQIRYFGQNSEVIPDKQGAPERRERKSRLNQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJ95</accession>
    <name>A0A8S1KJ95_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0230048</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the inner dynein arm light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJ95"/>
    <dbReference type="OMA" id="LYSQCFX"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183:SF0">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT INTERMEDIATE POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183">
        <property type="entry name" value="AXONEMAL INNER ARM DYNEIN LIGHT CHAIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8055178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="165" mass="19059" checksum="FB9BCE4A07E769FF" modified="2022-10-12" version="1">MEDFVPSLNSLVKYSTPVLVSVAGKNTGEKKDAQAKQQVRVNSPGRQEKMITQEILQQNLPPKEYRLKNGQLWVQTVLSTPATRIEVVQLQQELDKRLLSRGARETGICPIREELYEQCFDELIRQITIDCSQRGLLLVKYEFNIQQFLSSKRIQKLIEFIQNPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLT2</accession>
    <name>A0A8S1KLT2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLT2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="100" mass="11461" checksum="3F4BE52029161499" modified="2022-10-12" version="1">MGIICKQSPINDKNGLEVTLSQPPPIQQMQPKKPEEVKTQVPEKQEEPGQSIDQAKQENEPKRKEQKKMGKIAAVIDQEVIYENVQKQEKIKVHLIIDCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQ44</accession>
    <name>A0A8S1KQ44_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0230105</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQ44"/>
    <dbReference type="OMA" id="QRNFDYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="16"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8055292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="439" mass="50955" checksum="1615EBE2F2AA6949" modified="2022-10-12" version="1">MQQRTSTSQKIFNNQYQIIKKLSSGSFGVVFLGVDLLTKQEVAIKVEKEENEEVRSLEREVQILKKLDGAEGFPKYFWSGEDQGYNILVIQLLGKDLSYHFKQLKKFSLKTVLTLGIQAVQILERSHQKGVIHRDLKPENIILGTGREISKLYLIDFGISKVYRDSNGKHISFKDQKSFLGTTRYASIAAHLGHELGRKDDLESLMYILLYFLRGQLPWQNMVNVTDEERTKKVGEMKLSMEREVFKDQPSELQRNFDYIRKLQFKQEPNYKMIVQEFRRAAESSNITIDGNFDWTEIKQSTHYQTDSNQNQSKNNIPMNSIEMKKSIEKQLSGIIQHSSNNLLAPPPLGSARGTFQRDDIRKNSSLTQQSSINYCQSLNPNYQKSICEEPLQEENHYYDFDSVEISENYKADSSLYNKYNKIKLGIFVHFLPNQIKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQA6</accession>
    <name>A0A8S1KQA6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0250252</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQA6"/>
    <dbReference type="OMA" id="KDDDSCP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="78" mass="9228" checksum="4EA68F28A2B14BDF" modified="2022-10-12" version="1">MNQKSKKVHVAPQVELKLQNPLRQIRIDTYGQQILKGQKNHKIAFKEHIKTIHIIENWKLYNLDHVDKEDDSCPCQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWF3</accession>
    <name>A0A8S1KWF3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Aurora kinase</fullName>
            <ecNumber evidence="15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T0230136</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="15"/>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWF3"/>
    <dbReference type="OMA" id="FEYWSTE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001241">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="17">
        <location>
            <begin position="8"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="80"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="158"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="207"/>
            <end position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="11">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8055354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="479" mass="56009" checksum="E5D8A180A124E891" modified="2022-10-12" version="1">MQCSYKYCGLANKAQSDGVVSCPLCYQTRYCSQRCKDLDWTISHKLNCKGKQVRQSNVNDTQSTVKSIGKNIDDFEIIVKDGKSELGRGSYGQVKLVKDRQNGQLYAMKILNKKRIFEYWSTENLKREIKIQRRLTHPHIVRLYHYFEDKENVYLILELAENGSLFVYVRRRKRLPEKEAFVYFFQTCLGIEYLHKKNILHRDLKPENLLLDKQGNIKVCDFGWSAEANQQTKRTTFCGTLDYMAPEMLLNKPYDFKLDIWCLGILLYELIHGQAPFKGKTNQEKGQNIINLQTIEFNQSCSFEVKDLISNILKINPDDRLSLLQIFEHPFMKRNYESYGIDLNQYLNKEEKIENRSLSPVQEQLKARNFTLPQHNSSHELNNPSLQSTFSIQQKYSNVQNYKSINQPTTGTNTSISTYSQEDELKARVSRVSQRQQIAQQQREIGSYRQANSDLGLMDRVFQALGCLNRDKQQQNQQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWJ6</accession>
    <name>A0A8S1KWJ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">c-Myc-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0260181</name>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0560139</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AMY1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWJ6"/>
    <dbReference type="OMA" id="MMHYKEE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026060">
        <property type="entry name" value="AMY1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13168">
        <property type="entry name" value="ASSOCIATE OF C-MYC AMY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13168:SF0">
        <property type="entry name" value="C-MYC-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="59"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8057712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8076464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="11176" checksum="D1F7B4805E5E6AC3" modified="2022-10-12" version="1">MSFQTTDSKKEEFRKYLEKAGVIDQLTRVLVGLYEEPEKPNNAIDYVKKYLGSPVDIDVDKLKLEYEKLKDENIRLKREIAELKKELQAAQQEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYA7</accession>
    <name>A0A8S1KYA7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0280342</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KYA7"/>
    <dbReference type="OMA" id="QEAWYAV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035770928" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="231"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="40167" checksum="168A644937061C95" modified="2022-10-12" version="1">MQKIALLALVLAVTAAKFIDTHTTLAQINADPFGHVVLSAIKAHLQASTPANEVNMLLNGVGAGLVQDQNDHDHAFELDTTTNNRIVEDLEKEILYHQNQIASNTQLRDDTIEALAVSEEDIRVTIQDIANNEATYAREEATRNQQHETFVAKVAAIDDVIDAIDEAAKLIQHLSLGASFAQLKSKYEVLHKKLSDNTSHTALLQPVITALTELATHGVNQKALTKIAQLLSEIRQQLVQEKAAKTDVEDRQAAHWAEFSVHLSNEHTRLVERKAQLEVQIQEQKDTIEDAQSWIEFHTLELENSEERLAGQQAWYAVQSEIYETQTAERTAQQEIVDRLQEHISEKLSTTAQFIASRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L146</accession>
    <name>A0A8S1L146_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0300232</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L146"/>
    <dbReference type="OMA" id="QEQSEFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8060631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="357" mass="41648" checksum="D35060F7E228B331" modified="2022-10-12" version="1">MNRTPHMDQLISKIYRQRKNQTPNDYSREMYSHPNSHQTSNKDLLNLTSKQKLNKSCNYNSNLHQRLQSKAGADIKQKLLDEILRGSHSNISTSYLISTLKEKVSEKVNQKVQKYNTCQEASSFTIPQKELSPKPMFKILENFLFAECQNLKNQVSLNSFQELLDKMEIKEQSLKISIFADLKKLFIILAQSIRNDIQTQKKEIESLLRQNNSFKSQIQEMNSQLDKFKSVIEDQQKQIKIKNEENFISIMNTLRSHGVDPRKMLKKCQDNVETCKLKQEQSEFIDESQKYSSDDSIPFVDPQTIIKNEKKVIGLALNLNPLKDPKKAPIGYQDEFMANINEFSESWRQQALAEKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2X9</accession>
    <name>A0A8S1L2X9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S acidic ribosomal protein P2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0250212</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2X9"/>
    <dbReference type="OMA" id="EPYLHET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="63"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8057034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="10762" checksum="E568DB2F1BAC1D82" modified="2022-10-12" version="1">MANQATAVAYAALLLAGSKTPLTVENVEKLTKKAGVNVPSQLATQFVRAFEGKDIISLLSVGGGSGSAPAAQPTQAAAKPAEAPKAAEKPKDPEPEEDVDMGGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L7N3</accession>
    <name>A0A8S1L7N3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0340343</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="VEWVAIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF1">
        <property type="entry name" value="DISHEVELLED ASSOCIATED ACTIVATOR OF MORPHOGENESIS, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="3">
        <location>
            <begin position="39"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="4">
        <location>
            <begin position="890"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="798"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1275"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="485"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="731"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="798"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="832"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8063467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1343" mass="156196" checksum="40962917FC88E5EA" modified="2022-10-12" version="1">MGNAISNGKLISPISIDLQIKESQLTIRFVLDQKKLQTQQNTIPKDFDHQISQLIKELKVEPKLLQQFQNTPQNFKCLILNEYKNIIGKNLLQNLPNDEINYFTQKLINPTLQDLEQIRRYLKNLQIHNQQDQLEQFNNVINLLIENLFRFYQQTQQIIEKKKPPCYLYQIEILNILEILVKIEKNQQQMLLIPDSMDIILKNLHPTHVELTTIVLEIASHLCWHTDEGYNWVLKSLNKLYEDNEIIFFINTLQKSKNAVMIATICAFIVTLTESPTQEEERKRMCQQFIKSGIVDIYKMITNNIEEFEYKADELRFEVVLQQIIEQNKQKHQIFTDPNYIEEITLPMLYQRESRKIYDIGEFSNSIKIIEGQIEAFLENNKDIHIQQIQEPKKQEIKQHAKHLKTTFLTPQYLNLIQDEMGKANLTNLQRAVENIKHESKIRGTQIVTDFAEGISKYIDKLHLPIQKENENHEISQFESMKQLIEDLHEKIISLEYEIKDLNQKKQSNESFVDVLQRKIGDLEKKMKSDQSILSQLNEQLASKNKDFRALQQENESQQKTIQQLENEVNQLREKLSIMQLAKAQKMEFEIPPQRLSHKSDNQDDLRQQLDQLKQELQQQNQKFINQENENKKFQKLLKEQNQNLLTKEIQLTEKNKECRELQTTNEKLIKKKYAKKIELQQLREKVQSVNYVKTQATKIQSELKTIEQYQDEVIRLNGLIEEQAFYLEQQKKQTQKFDEMRENQKVLENKNNKIEDEYKKQSINTQILQEKLQQLQQEKDNLEKKLQNQQLITQNSIQSNNTFQSPNPTLPSTNQINPLTPPISSNSQTGPAPPPPPPPPGVKTVSTPPPPPPPPGGPKPPGPPPPLGGAPPPPPGPRPPGGPDPQFGGKQKHKPNVQLKQVPWTIIKPEQIKNTIWEQIDDTKLKLDYSIIENLFAVKPTSSNINTQSGINQAQKTAKISMLGPERIKNLEIVLGKLKMSNQLIVDSLYQLDETVLRPNIVESLITAMPNDTEVGLWQDQDQSNLALPDIFCINISSVKGYDFRLLLSLKFKFNYKELVEDIQSKINIFQNLIGKTKNDKSTKVVMEYALAAGNYMNGQSARGGAYGFKFEMMEKLADVKSTDNKINLLMFIIQKAEEDLQQELVLVDENIDDIELAAKTPLNQLNADLSELKKNSKIVDKAMKSKVSHPIQDLVEEKLQNFYQQVISDIGDFENLLKQLENQYEDLSNYYAESKVNFDKFFEKFYKYKIVLRQAKQNIIKIKQAEQKEQEKKKKLEQQQQSQIQKKQEDNSLSGLKKLDKDQILNEVAKRRESKKNSLMLLSNVSVKQQIQPSQTETYDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L7N5</accession>
    <name>A0A8S1L7N5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E2F/DP family winged-helix DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0320052</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E2F/DP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L7N5"/>
    <dbReference type="OMA" id="IVQVNEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000360">
        <property type="entry name" value="Transcription factor Dp-1, a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="3">
        <location>
            <begin position="154"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8061632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="364" mass="42957" checksum="FD3991AF56A1BC18" modified="2022-10-12" version="1">MCDQTPIEYRSEFESSPMNFGQPNMILFQPYSPLQLRINCSPNNLLIQDYYSAQPNQVQEDLFPFDQQPLQKMFELQISNDESPQIKQIVIKQSNTKQSGFQIVHVNTQQMTPQKYNNQHMNRHHALQKNISYSEKIEKKNYNYYQYQEQTPLRSQKGLRNLSVKVRDIVMELKSTTYKDVAQRLIQDLGQDGQLLDLDNPKDEQNIKRRVYDALNVMIASKVLKKEGKKVISDMQGKTRTRRNETDNQKEELQTLKSIIKEKKKRLKELFTKTLALENLIKRNSTNELKNKFMFPILTFQGHQQQIKLRMDSKVLKIMSQQRLALNADLDILVQMKMQQIYKNDDLFIPKELLDIVQVNEFCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LA28</accession>
    <name>A0A8S1LA28_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ODAD1 central coiled coil region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0330164</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LA28"/>
    <dbReference type="OMA" id="KQMVDQD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051876">
        <property type="entry name" value="ODA-DC/CCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049258">
        <property type="entry name" value="ODAD1_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21694">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21694:SF18">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21773">
        <property type="entry name" value="ODAD1_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ODAD1 central coiled coil region" evidence="4">
        <location>
            <begin position="182"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="583"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="129"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="381"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8062493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="610" mass="72111" checksum="7B2798EB7815DB27" modified="2022-10-12" version="1">MNQTQKSFDVSKGGEKVSDIESLKRRTYKKILMMSSMNKNIMKYIQKAFQLGPKYLTIECLVEEVKTTPLNELERYGATILDKDRLKELQELLGKIKQMIDQDVKQLSSDQLVQLIKYFVLSPVEKTYIQKIEAELAETKNLDEQIKAAQTRQKEQQDQKIEEYRQEKNGQQIIKQNLQSQQHQISVHENKLEKANQKLNEIVAHNKQLREKINQLRKEKNMVEEISKNLEKELEDKKKNVEETIKSAGQAYYYRNKAEEELTKLQKKAEAQKKEFEQECESLNEKIQHDKKFKAFIQSKKKEQEYLDKLEKQIAENQEIIRQKSASNAQIDKEYMLSASKEQEIKDAFERIKQETGIHDKKKERESKQLLTVFIELYQNNQIMSQFVKELQETVEELERQIEEKKEEIQMYSTKGATNDNQRREQKMALSNKIQQEEKKKVILKAQYEKSIETINLIKKYLEEVFQAIDVDDETIKKLKSAAITEENMVHFLGILEQKGLDAIQEYARLIAEQLKLEKGEVHGLSSQVDDLNNIIAYENANIMNYYSQASQFRQECPDDVLSFNEEENDMKQRWFQEEEFKKQAMESISKRSGPKKPIKPKFKEREQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBR9</accession>
    <name>A0A8S1LBR9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0350098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LBR9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="248"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="356" mass="43224" checksum="3ACBBD1664ECBC6A" modified="2022-10-12" version="1">MSTCRELIVCQNCAKDLISQYKELYQTFLSRQEKFLFLQSDLKQYRIQCEIHTSNLIGSKRNDLFSNKKQKSSLNLLGQNRLSLMDFPKFLFKGEFQIIIRADVFKAQVNYFLKQMQVNETINNEKEIIMHINEIIDPQDNINFNEFIRQRDNQIIINYDYILFDKILKFGTTVLDIQIQDNFKSKILRAISSLILEQILNGRDQFIQKYHLKINFIRILKQIIIQKIENRIQQIEYEKQQQMLIAYIEQEEKQIEVKKEKQRKKRQQRKLKKKVNQQLEQLEEEEEDDEETKNFFCDINFKTQKCESSHSDKEIITELLILKRNINEINQKRKQLRETIRKEWENYQKQFQSLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LC55</accession>
    <name>A0A8S1LC55_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0350017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LC55"/>
    <dbReference type="OMA" id="YRNEWEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035724501" evidence="2">
        <location>
            <begin position="21"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8063542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="354" mass="40607" checksum="F4C99EDCD89EFAA6" modified="2022-10-12" version="1">MKQVIFLVLLLTLTLQTSLQSKVDVVMAQMDKMAIKNEFSKQLAGLIELKMLQSSYVEEVLKEIKGIRDQLISDQTVEDQEFAKKIGQLNVEIEILEIQTEKLAKELQRLNQQIADLNEDISKLIGTQQSQEKQLSTLNSKEEDIRNQYKSEIETISQRTTNNIKSIDGLNEMIAKLQQAVFAEQSKTTVLSQQHTKQYVDELANSLGPNHPITALVSVTTKFDVPTVTRIIQLLENIRDQRIQENAGANEYVAKVTQSYEVTLKEVTEVRERLSADYSRTLITLKRRNEENALSTKSRNQIQKDLPIALDLLQQYRNEWEIVQSNYNLRSAKRENEVKIITQAYTIVAQQVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCV5</accession>
    <name>A0A8S1LCV5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0360092</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCV5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8064371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="26393" checksum="1B62C199CEF9EB98" modified="2022-10-12" version="1">MNNNNSQENNNEQQEEPMTEEERQFAEELDQKIQNAQQKLDQLGLSQSRLNWLQDTIKKRQEENPLTEEEKKEKFQQIADKAIDIPTIDRQGRKIFTLEETLNNYNNAWGILKYRELPICWSNLKDKIIFTLPMTFMAAYTYEAMQLKKINLSLKEMKFKLLGRSWGVFFILGTTFCIIDSLYFDDFCDIKSYHYQTNDRKKLARELRSQLLNKPKQKDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJV0</accession>
    <name>A0A8S1LJV0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0390090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJV0"/>
    <dbReference type="OMA" id="WDITIGF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8066442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="311" mass="36057" checksum="C2D5B1EE7476AB37" modified="2022-10-12" version="1">MMRYLNLKLVQLYGDQIIKMIALLFCVLSFLHLSMAKKCQCNEIYFEDLCRDARGCYFETKSGICEEINCLERTSEDCSYFAGKLRCYWDITIGFCQELSGCEELQENEKVDSNEENCQEIDCRWDYQQKICVSDADQRMCQDYDVQFCIGAIQVVGTQKSECVLNGENGDSCIALEKCEDITYNKSCNKLYCKWEDDECKTKTCSDYSIQECPSFNKLSQKQCYPSHSGCVEFVCSDFGVKLQCQNHPRCFWSQNLNSCHQQTCDKATYATQCLSFSYAVENTECKWDSVGCHSCYQIALVFYLIIYIFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LM89</accession>
    <name>A0A8S1LM89_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Dynein heavy chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PPRIM_AZ9-3.1.T0400212</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YRTLIMA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000063">
        <property type="entry name" value="dynein heavy chain 6, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF36">
        <property type="entry name" value="DYNEIN HEAVY CHAIN, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain linker" evidence="15">
        <location>
            <begin position="905"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain linker" evidence="15">
        <location>
            <begin position="1503"/>
            <end position="1730"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="16">
        <location>
            <begin position="1853"/>
            <end position="2172"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA 5 extension" evidence="20">
        <location>
            <begin position="2314"/>
            <end position="2439"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain 3 AAA+ lid" evidence="21">
        <location>
            <begin position="2651"/>
            <end position="2738"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="18">
        <location>
            <begin position="2790"/>
            <end position="3016"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="17">
        <location>
            <begin position="3030"/>
            <end position="3373"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="19">
        <location>
            <begin position="3400"/>
            <end position="3597"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="14">
        <location>
            <begin position="3830"/>
            <end position="3942"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="22">
        <location>
            <begin position="3973"/>
            <end position="4089"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="23">
        <location>
            <begin position="4115"/>
            <end position="4368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="755"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="863"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="3059"/>
            <end position="3114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="3261"/>
            <end position="3340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="3587"/>
            <end position="3621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF17852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF17857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CAD8067301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="26">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="4370" mass="517268" checksum="07D8D51B7229311A" modified="2022-10-12" version="1">[encoded data, 4370 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LPC3</accession>
    <name>A0A8S1LPC3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial import receptor subunit TOM40</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0420275</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LPC3"/>
    <dbReference type="OMA" id="WTEMGNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07305">
        <property type="entry name" value="Porin3_Tom40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037930">
        <property type="entry name" value="Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10802">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8068669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="38243" checksum="B0851D84FFF9B141" modified="2022-10-12" version="1">MAAINKEQVDMDNPAFAQQDPHLKINDEEEMSLPPQFRNRYPRRPPLFDGLLNNVRAVTKKVEHIKGFKFEVAGGLSNNFHLAHSWMIPPSNKGKAPNPNPMKQPPVPSYTLAAQYLGGELRTPFDQPSYIMTGRWDSTGKLEAAIIKKLNEMFNFRFSAFYLDSSPNNAQVHFDCDISGDDYVHSIKLGTGLYSFNMMQTIGKRVVLGYEMMTLTERNMSLMSYAFKFGISQKQNFYAQYVGAADQLILAYNHRLLDKAYFMSELEYSNQSGESRTILGYRQKFATSEVIITVNSKMKFSSALTLQGFAYQLKLCAIADYNKDSYKFGYGIAMGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSF7</accession>
    <name>A0A8S1LSF7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0430205</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSF7"/>
    <dbReference type="OMA" id="ARNSPQW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="67"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8069115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="653" mass="74244" checksum="EED6EFFA8A301DAE" modified="2022-10-12" version="1">MQAKREYLCPITEPKPDETPIYRHPMAKDGFLPPLDNRKVNMQQQLLIQFEKFSDKKTLGTFNEETQQYDHITYKEIQEIATSLGSSLCQQDSIKEVKDQQNNVIKPVGIYLSNRREWTLIDVACILYGFTSCPFYDTLGVDSITYSMNITQVSVCFVQPSTIGFLAKSNLPYLKTIVTIGAQDPNILNTLAEQKKEVITWDDYIKRSNGKIQPYPNLQPQHPLTLVFTSGTTGEPKAAIQSHLNFTSMIALFEHQDKFALTQDDVYLSYLPLPHTFERVVHLAALSGGAELNYYSGNIQNIAKDIQRCKPTYFCGVPRIFNRFYEGIQAQLNSFPPEIQQKFELALAAKIKYFRATGKTTHDQLDEAFVKTRAILGGRQRIMITGAAPISPKILEYLKMTFCCQIIEGYGQTETTAASFLTDFNDPVCGHIGGPTISQEFKLVSVPEMDYLTDQVVDGQKKIRGEVCLRGPSVIKSYFNNVQSTKETIDEEGWVHTGDIGEIVDGALKLIDRKKNLFKLSQGEYVSPEKIENCYLRVKGISEIVVFGDSLSNYCVGVIVPEQTFLKQWATELNIQGDHQQLCQDKTMRAHVLKLVNEQGKQDNLNGFEQIKNVYLEPRPFIQVGILTETMKMQRHKARQNYQDIIKFLYQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LWB7</accession>
    <name>A0A8S1LWB7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0440295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LWB7"/>
    <dbReference type="OMA" id="KIGMTPP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="517" mass="60936" checksum="231F45D836242EEF" modified="2022-10-12" version="1">MADNDDYEIQYYKYLEKIGMTPPNVKVRQTPQKQNNSGISQRLLFQNSSQKKELNYQRINSIIVLNNQLEINLTNKQNQSKILNISSNQESKSPIFDIQTSLIQPNEQKNEKGLSQIDEFADEYQQENSNKTNCYDQNIRQEQNNNNINLDFSSIQLKPLITNKFHEDNQNQTEQDNQQLQQCQDNDNQISLCNNQELNIKQNQQSNQSCQHDPSIKESNQELSKQNSPIKITIDQNQDEEINQEQLQQSIRIQNENIEKINIVDLDVNNTQNLESQPQIEMNPKIETPPQEINIQEQKQDEYPCQIQSKSVDINFQESSDIQLQNQINDIGQTQIQVSDNHLIINQTMEIEKIQESQINQEQQIIKQYQNNQESHPLENKLNSDQQNNINQQQSVEQRLIQKKKKKQKKSRIINKKKKKEKKNKVEQNEQKLNDEKKQSQQIIDCENKQISQIEEKPNNEQIIIEQEQKNPICNLLQSLIKGFYGENIKINWNKYTFLKKKIQKKRSILLKSKLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZ07</accession>
    <name>A0A8S1LZ07_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0490113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZ07"/>
    <dbReference type="OMA" id="QQPCKTW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="139"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="359" mass="41430" checksum="6C717E4332671CFE" modified="2022-10-12" version="1">MNQYNIPNLQPEQIFLNGETQQDNLKQFIISNECNSQKIKDTQSKVNFQLILQYTINEKCYTLDIDKSLSLIVIGGINGLILILKFQQNQLKVQKLVKAHRLVACIVVIKNSNQFISSGDQIIKIWSLQGIQLRLLQSLYGHSRPVRCLLISHNEDLIVSGGNDQKIKFWIRNANACKFNQQLLEHSNFICGISLNELQNQLISTAWEEPIIKISQQQPCKTWVVIQTIEVNLWGQRICFISEQMFSFQPFNGQTMHLYQFQPKNNIYALSKTIDVIQSKEGCTNLFPKQFIKQQSILINKNGECVNIILIKNNDECIIQQQIQFGHYCINGNISHDGQYLITWDLCSSQIQIRQNLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M0C1</accession>
    <name>A0A8S1M0C1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0470091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M0C1"/>
    <dbReference type="OMA" id="AMKQKIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="110" mass="13589" checksum="488DB4FB25F90192" modified="2022-10-12" version="1">MKTIKIAIQKDVFFCQRELNRFEDLQQEYQLLCQSYQNKDELTFYYVDEEGDKVVISNDYDLEDFKKQDPKFQKIRVDIRDRNNQREKIYNKELTKDAMKQKIMQLLQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3V5</accession>
    <name>A0A8S1M3V5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0490171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3V5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="67" mass="7780" checksum="AD61C2AB59F588B0" modified="2022-10-12" version="1">MRTHCFSNIYLKKEIYPAQFMCGILKGVLNASGFTCQVSYQFKTDERGVSYYPHTVFILSFEDARDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5C9</accession>
    <name>A0A8S1M5C9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mre11 DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0480066</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5C9"/>
    <dbReference type="OMA" id="QEDFRID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097552">
        <property type="term" value="P:mitochondrial double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007281">
        <property type="entry name" value="Mre11_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139:SF1">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04152">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01347">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mre11 DNA-binding" evidence="4">
        <location>
            <begin position="288"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="612"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="615"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="628"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8071816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="670" mass="78988" checksum="33733DB9E773990E" modified="2022-10-12" version="1">MQINRNQFIKFLVASDNHLGANENVGPKSNRYQDAFDAFEEVLLIASQQNVDFVILGGDLFHEKHPTEHCLLKCVDILQRHVFGDNFGGIQMEVNSLNYQPNFSCSNFNVQLPIFIINGNHDDIVTERNESVSILDILHESKYLNYIGKITDQSNVCIKPIVLVKNNQKIALYGLGYMKDYQLYKIINDGKLVLDQLDENNFNILIIHQNKYKGNHFQDEKNFIDPIYFKNYKIDLLIWGHEHEAIYTLDTCEHFQVFYPGSTVATSIIEYESLIKQAGLFTLTKNQMKFESIKLENSYRPMIYKNIELNELIKSTENNSNISNQEIAEKLLFDFVEKELINYYSTSQYRQKKPLLRIKVEYSGFEIMRMRFIETKFADRVSNPDQIFKFWEKKSNLQAQIQKRKEQAHQLNQQFDNILRNKVEINSQNQTMMNEFSNMLSQKLYQQNFQIIEQGDFLNILDNFLSSTNKEKSNLFNDLYPKTIEKFKDNIIPQYNNKILQIVKKQREYQESLIFQIRDAIIKNFEGKTKYEQLYCTDFGFDISQTFTKFQEDFRIDTNKINLNQVNKSNASFQTMPSKQIQFQKQENNLQSPIIIEDDSIPKNGQTQISIFGQDEQEDEDFEEKQDKKGKKGPKKRKPLKKEDDIVILNKKQKQKIELQPYKMGMNKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M741</accession>
    <name>A0A8S1M741_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0540286</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M741"/>
    <dbReference type="OMA" id="GMCKRYT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="105" mass="12183" checksum="782D544B575099FF" modified="2022-10-12" version="1">MATGGQILRYNGGTCYAMCQDVFSWYNPSIQICWKGCDYATGRVNDPVLRKEAEDMCKRYTAEAMWTKKGELDNIEDLRIHADMFPENPRNIYRACLAGVRRQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M7M1</accession>
    <name>A0A8S1M7M1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0550237</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8076107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M7M1"/>
    <dbReference type="OMA" id="GYSEFYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000071">
        <property type="entry name" value="Rodhanase family domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF17">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="114"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8076107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="260" mass="31146" checksum="0A22E2F84A469F66" modified="2022-10-12" version="1">MEAFLHNLIRKPLLNQALFTLNKSNQTKEINENYSKQGLLSRKKIKIDQIDNYENKLEGAQKFKYDNKEQNEEVGLNNDMNITFDEQRQDLMLEYHLENSIQYISGQTLAQTMKLYDILIYDCRYLYEFQGGHIQGAKHLNHFTNLFEELFGATKQQNKIVVLYCEFSIKRSQEKYFEIRKLDRSMNKYPKLTYNNLYLLSKGYSEFYKKFQNMCNGNYIPMNDILYEQELEKEQQLRNIAKQKNRLKQIQKITGCLNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M873</accession>
    <name>A0A8S1M873_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SMP-30/Gluconolactonase/LRE-like region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0520049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M873"/>
    <dbReference type="OMA" id="ARYEFAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000174">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051262">
        <property type="entry name" value="SMP-30/CGR1_Lactonase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47572:SF4">
        <property type="entry name" value="LACTONASE DRP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47572">
        <property type="entry name" value="LIPOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="2">
        <location>
            <begin position="25"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8074041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="289" mass="32292" checksum="7C4B3BB4C01B8A34" modified="2022-10-12" version="1">MSAPQIIIEQQSQLNSPIEVDGAVYVAAQNGEIIQYKDEESKTFFHVASQPHSIKMDNSNKVFYVANMARQCIQKFCQEENNDEIIDFINEFEGMPLLGPNSLLLSPKNNMIFFTDSGPFGETAIDNCKGSLFAADLENLTCKALALYCLSFPSGICMGNDQKTIYLCETGKNRILRFVQGNAGIYYFSVYIQFQGRFGPMACAVSSNDLLYVARYEFAQVTEDGLISVYNQNGINIDNIILPQYPELTGMAFSVLQPSVLYITENSSKGKCLKLNVTPPDKKDKDKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8F0</accession>
    <name>A0A8S1M8F0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0520090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8F0"/>
    <dbReference type="OMA" id="CKFKNIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="203" mass="23485" checksum="34630BEF51DC2C57" modified="2022-10-12" version="1">MDSLPAHLREQKKQISLILQSSQNLIQLSQKLDQTSQSEINFQPGPRLNPNQIESKSGDTGDLFESGNLLQNTENHVQTLFNQQFTKSGSLLNLYCPHVCLTQEVENSLFEQIKLPSTCKFKNICKMNCQMKFNPAYTNVQQILLYQLHENQIVELRSTLLKNNSQKIKVCDGQGCEFYFILDNQLQQRNYYCPLCLESFELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M960</accession>
    <name>A0A8S1M960_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0570233</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8077138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M960"/>
    <dbReference type="OMA" id="CRCIRCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF4">
        <property type="entry name" value="TONALLI, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="4">
        <location>
            <begin position="491"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8077138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="587" mass="69521" checksum="F5FC5FD88FE5AC68" modified="2022-10-12" version="1">MQQLLPQPLPQTAPQPVQPLRSISEILQAIQKAHSALKNANSDFEERTKKFSGLTSTMTVNFECSLTKETLKYAVFLYECFKTFKHYDGVFDLEQFLKLNYSKLEQQQQKQFSCPICKTVTKFEKIEDAFFPHVIINELRTKCGEFPRQIMYNFIDKTYYPLHKGKMGIAQNSYFEQIGKFMKREIKPENQFYQMVKNFNVNCSFLFYNNCSLSSIKVMIPVRSQQCKHFEVYDLTALLIHFDKKEKQFQCKRPGCNSIIKEEDLILDQDLFNSCLKSYSFRFSFIYNKEKRKLEDILEEKQDINLQKYRKIDQTQEYLNFQSKIYKIVDQIYTSPINQDKTEEFYKKYSLENIKNQNLELKFCQFTDQRIELPCRCIRCEQVQTCDLRYMSCILNQFQNPADIKIGDKVTDKCPLCNNPFTKKIKPKDTPIFEQVYVDVKMMNFFTITTGFANINKKDFINYLQDKVITKVILKKDQESILKGKQAITSMKLECPISKKRITRPIRGNNCTHAQPYDFSILDMHQEGQINLNERNCDICQAKFDYFSEDTFLKDQLDIFYTEQLEECDTVRLNIMNNKISIKPKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MA04</accession>
    <name>A0A8S1MA04_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0530073</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MA04"/>
    <dbReference type="OMA" id="CLIRYFY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052788">
        <property type="entry name" value="RING-type_E3_ligase_ATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798:SF97">
        <property type="entry name" value="ALCOHOL-SENSITIVE RING FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="114"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8074633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="159" mass="18524" checksum="A9C570BE5621C5BF" modified="2022-10-12" version="1">MKPNLQIEYQSQTASPIIQFFSTILKEERENEYKDDHEDDDDEETPIKKAIIIIIALCIGLVILGILSCLIRYFYMRHKQKRIAQVRQQSEAAYQQQNIQIESLCLQTQELAQCPICLMPIPNCYLIITPCHHSFHQACLNLWLQVEKICPSCRTSLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDW2</accession>
    <name>A0A8S1MDW2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0600102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDW2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8078537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="50" mass="6076" checksum="E044E0B543791C10" modified="2022-10-12" version="1">MFLICKQEFYTRNNMYDRNIDVKYIGENAQSIYCTSQFEAQENIDNTRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MIF6</accession>
    <name>A0A8S1MIF6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T0610246</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the JMJD6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MIF6"/>
    <dbReference type="OMA" id="RFHEKEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033749">
        <property type="term" value="F:histone H4R3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106140">
        <property type="term" value="F:P-TEFb complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050910">
        <property type="entry name" value="JMJD6_ArgDemeth/LysHydrox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480">
        <property type="entry name" value="ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480:SF32">
        <property type="entry name" value="BIFUNCTIONAL ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjC" evidence="13">
        <location>
            <begin position="162"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="368"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="374"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8079289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="400" mass="46681" checksum="F00F92027D9759B5" modified="2022-10-12" version="1">MEVAKKLKLQSQRFHEKEMSWKVQEQVEEIKLKDRGHLRLKEWTKRNHYEKNMKDLRKQYEKYFTITRVDGRSLTSEQFVNQFEIPDIPCIIANTVDDWNIEKYWTFEKLYQLYKETSFKVGEDDKGKKLRMPFKNFLEYLVYNKDDSPLYLFESSLEDMKDGGADIINRFKLHKYFSEDLLAMVGEKHRPPYRWFLVGPQRSGTTVHIDPLMTSAWNTSLQGHKLWVLFPPDIPKCVVKAKGLAAKRLIDPVDLDESIDYFMNALPKLIEQEGADNLKIIMGIQGPGDTIFVPGGWWHAVLNLDNTIAITQNFMSINNFDKTWRSVRDERPKFSKRLLQVFQDQRSDLYERALKLDEIDKNQFRVLRQANPLSSDESTGSSSSSSSSSSSSSSSSDEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPU9</accession>
    <name>A0A8S1MPU9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0610060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8078976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPU9"/>
    <dbReference type="OMA" id="MHYIPFF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8078976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="30770" checksum="59922A388E8B491C" modified="2022-10-12" version="1">MNKDDIIAFYGKREVKETYNYDILADDRVSLFSSTIESKHQNQKTMILSSQPTIYCSLSICSFLIALSNLPEEDRIYFFYDDPLSYNIGLVVCMCCLCILGIVISEIIKRSFTNKQIGTLPFLTKKQHKMNFNATICLAGQIAHLLLLIEIILQAMPTQLFQHETLVDLSFLLNVVYFAYANSFLEKKKATQMHYIPFFFYICITLALLCQNNSMKLQYTHIWKVQTERVILTYMGIMLQGIQFQLFRPAIKEMERNQNILAEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MRK3</accession>
    <name>A0A8S1MRK3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0680171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MRK3"/>
    <dbReference type="OMA" id="IIQPQYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8082810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="35816" checksum="F1FE9A9A88C2B3BA" modified="2022-10-12" version="1">MIYPGLSVVDYQLQQYEQYPLYQNAYDFNIYPQYHYTPSIKNYESEQKEEFNKKNKNRKIKNNQPKELLPKIKEQIEEPIEQNEEILQQIIQPQYQIVDIQQGALLPKLQSHLTTEGHIINSKQIIKSTKITKKQKKENVNTGHWSTFEHTTYVNFLSQYENIMNSSMMKKTSKIFKQMSELIGTRTPSQCRSHHQKFNPYALRGDNGKRLPRSQRSRAGRKKKILFNNLLQTNQLIDNDLHGYMYEKQNYYNPFIIDYQFNENEIFIKNQNQLIQTQIQYEDYLKLRQDYHNYLEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MRZ1</accession>
    <name>A0A8S1MRZ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0620261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MRZ1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035897510" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="17472" checksum="1500CC96D22E4C5E" modified="2022-10-12" version="1">MMISIFFLYIVLLNLRQRKKNGEVVKEKQYLLINGASLDYKFHKKKDGKFFKLIWQLKYLFSIRLVPLNKAHPLISTIEQFRPIRLKVILLSQLKSFLYQNLGKSQQKKSRKNWFCKQDGCQYHVGQVIEKLKEKYYIRSSIQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MU33</accession>
    <name>A0A8S1MU33_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MU33"/>
    <dbReference type="OMA" id="NDWWVIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF53">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_67, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIR" evidence="1">
        <location>
            <begin position="132"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="1">
        <location>
            <begin position="207"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="387" mass="44898" checksum="BAF83C96B202B0A7" modified="2022-10-12" version="1">MQQEVNNQAEKPQRPFLHQELVHLFHPQQKVYLTDTNTENKWGLILSAQPNLTPNTIFILEKYCPPFSMFKSPNYFNTPIIQGDLIRIKSFCSNYYITSIPDAQSPITKQGLIVLAKQNDAKYKGGEQVFILQNISDESPYMRIVNTETGWALHTHSSKLKEINNHNEVTGFKSRDQNDAWNIEMISNDMREGIHQQLIQEPLPRKCQNIRSSDTVIIRNGFTGGALHSHSAHYKYGTKQQEVTWKIQPRDLNDWWVIYKQKSHKQDSDEQIQFIENNSIVSFLHVQTKQLLTHSQGCKVKNGDYLQVCCSKTSNEEFKIELIDINETSNNLTTEKPFRIVHIPTQQFLAALERPSSQSTFQGEIVLTQKYDWNAVWFIEYVDSIYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWG0</accession>
    <name>A0A8S1MWG0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0700172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWG0"/>
    <dbReference type="OMA" id="WFMFEYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8083662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="109" mass="13431" checksum="A768DEF8D4526300" modified="2022-10-12" version="1">MNTESQAIDLNRRILKSSLTRQFTRSPTRSRKRIISLPPLEAAQNQYQKLKQRNISYHKESPREVFIFIISVVRFNLFDHQRIKWFMFEYLSIYQSQRREKIFFKQQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N8X0</accession>
    <name>A0A8S1N8X0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0760242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N8X0"/>
    <dbReference type="OMA" id="ICAKESH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="325"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="420"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="462"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="536" mass="62022" checksum="B3D6421A78B0083B" modified="2022-10-12" version="1">MNDLFSKANSIKCHIKHHSDLGLGISVMCLDEKCISNKKAICAICAKESHINHKVISISKIDQCITEYQERWVQNKLKQQLVNDFETITEEIGQSFLKYGSQFSQILDEINQVQNIYKTYIQCEEITEVVLQKLGQELQELTRLDAGSLNLKFSGQKQISQLKQFVLEMQSLNILLNKAQKSNEMLQSIILQTQERDQLKQYLENLEKLQYEQTKDQEINAVNEDSFNLCDKNLIKLKLDYYNSVTAIDVTNDGQLFAGGSNWNNKVVVYDLDSMIELKCFICQSDRIQALKFSPNKKWLLVGSGRQSDIWMWDINNLDSRPLIFKGHNEGINRFSFSNDSKYFCSVSGDRQAIVWNIENNKQVYRLNGHSQAVNGCSFGGGNWLMDMDKENQNINLIIATIGDDKNIIIYKNQKVLKKWQAHNDEGYCIEFSYDYEWLISSGKDKFIKLWSTRNFILLKQFEGHQRGIWTCGFSPYSQYIASASWDDTIRIWNVNNVNDIISVNNEFVNLPQVCINGFSRCVIASSKTVFNIWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N998</accession>
    <name>A0A8S1N998_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0770106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N998"/>
    <dbReference type="OMA" id="NIDRGAW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="140"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8086806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="215" mass="25343" checksum="B0CAD53A036B600C" modified="2022-10-12" version="1">MARKILVDEWLKGDVKDVEFKKKTTGKKGQLLVELQKSYKGDSRFKLDEKFNDDLNVEKLNKKFKDLQQDLIEESSDEEQDLEKEIDENQLQIKSYQDEVYQQLQILAKLMPDDAAFISYQKKPDKKKYNLIVPKFDPNNIDQRLIKKNEEKKIEQAKKQIKTEIVKGVDKKTIKIEKANKLLQRVKEKQEIKLNIDRGAWKNIIQEEGSKGLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NA29</accession>
    <name>A0A8S1NA29_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0840175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NA29"/>
    <dbReference type="OMA" id="ANCDYIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8089857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="123" mass="14256" checksum="A82DD082D2ED70CF" modified="2022-10-12" version="1">MNWLCGGNDGNCLRNAIYILGANCDYIFCDIGYSIQLDLMQKYELNTLKIWLWDGDYRFSTIKVFIKLENTETLIYDSNLAQSIVTITFPDQIVGSFRIYNTAGNTRNTGLYLIKVEAYYKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NA82</accession>
    <name>A0A8S1NA82_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0760223</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NA82"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8086463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="98" mass="11359" checksum="0550173AEBAEFD85" modified="2022-10-12" version="1">MNDTIEFDDCLNEVTSQEKDIINYLQELKLTFLTDPQQKQFKFEQAKKQLVFSYRSLDSDTDGSEMTPPMLVKTSSLLLTKSPLRQKRLSIVSEQPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NE52</accession>
    <name>A0A8S1NE52_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0880037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NE52"/>
    <dbReference type="OMA" id="AYENWEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="145" mass="17264" checksum="18BB08301FC9B08B" modified="2022-10-12" version="1">MKRLLRNAQILQKQSRYYHHDPASRALVGSPQEIAPPIHFKLYYLPDNVAKTGQGMGHPHYSAYQMEGITTYDNWEYKYYGQWWHIGSVFWNSLMLFYPLLLWFVIEQSEQEALLVKDDIFKKYFNEAGGFYQYTFIGPETTLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NE88</accession>
    <name>A0A8S1NE88_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0870030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8090920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NE88"/>
    <dbReference type="OMA" id="DISTHNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8090920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="377" mass="44038" checksum="E2E13875DA65AAAC" modified="2022-10-12" version="1">MESRNLTQFQHYSHNGTNRYSIQRIVYPQYASGLVQEETVSQHRDSTDEEQFIEGECFIYIGGQQIFTIELKQPDFVHQQLCIDLTKCQCPLECLTAFPLPNQFQNPQRILPLSTGILVVSSGKNGQQINLFDDQSKEININVQINKRIIWMNSRYLQEVIILIVGFNDSSVGLFEIDIKDKQIRLLHEFNFEQLIIFNDISTHNQIYYIALTSYNGIISILSIDLFFNIQHHVHNINRITSPPKLYFQHNKASLMVPIQNGEMLQYLEIGNHGLSKWNVIKYQNNNKSMITAFDIEPKKCDVSQNQSEEFEICMIHFDGTLSFYDYADNSIYIEISKQLNGSRFFVSNMQLQFKQFILMGSDDEIEIYQMSFYHIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHV4</accession>
    <name>A0A8S1NHV4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YCII-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0810178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NHV4"/>
    <dbReference type="OMA" id="YYHRIPL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051807">
        <property type="entry name" value="Sec-metab_biosynth-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33606">
        <property type="entry name" value="PROTEIN YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33606:SF3">
        <property type="entry name" value="PROTEIN YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="13627" checksum="6C56610695908A07" modified="2022-10-12" version="1">MITNQIKYSFALYQKYFLVSQQYIQDMYYHRIPLRQEHEKQLQKLISKGAQIIGGGQFPYNGAYYIINTEDDNNIKTFINNDPYTQNNLAKTTYEQIYPLIDNFDQLSQLYSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2024-11-27" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NIW0</accession>
    <name>A0A8S1NIW0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-grasp fold RimK-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0850073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF0">
        <property type="entry name" value="BETA-CITRYLGLUTAMATE SYNTHASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp fold RimK-type" evidence="1">
        <location>
            <begin position="114"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="225" mass="25750" checksum="7B4087AC344022BA" modified="2022-10-12" version="1">MLNENIIILYMKNPDPIFYGMMDDLVIQTLKKQNATIIVTELEELSVIIGEEFIFLRNGEQIQFDAFLGYGYMAPKHYLDYMYFNFAAYTAGKTTLYSPFTENILQDKLLQYAKFSKCQVPFPRCSASFSVQQFKLNLSQFESKAIMKEVIGYEGTGVKLSCNKIQSTEIFCKSLWNGEQAIIQDFVSDSVGRSIRVLVIGGRAVSVTEFQDNIXIIKDINHILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJV9</accession>
    <name>A0A8S1NJV9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0860184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJV9"/>
    <dbReference type="OMA" id="ANDSCNR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09487">
        <property type="entry name" value="SAM_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="2">
        <location>
            <begin position="107"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8090736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="285" mass="33204" checksum="2DEC0F74E0948139" modified="2022-10-12" version="1">MQLRSISNKKKPSQLTQTTQLLEKETKHMEEQLQQLKQIMQQEKFKRETEKPTGQNGTRWKAAKIDKGLRNYTQNLLQKKNSFEKKSIPTVVRPTSAKLQPLEKSIKTPAIDKDIEIFFTQVGLLQYKEKFQNYTIQSLKNVTVQQLREMGILPGHQIKIMRALKEIPENIQQMQEDSFCQSRVSMSVSGVQCDQTKLACWLCYKIFDDAEINCNGRNFCSQQCLIQFNQEYMIKCSKCNKKFYKNDGCVVYDNYYCSQSCSIDANDSCNRRGLISINELCRIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NNZ7</accession>
    <name>A0A8S1NNZ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0800203</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MGR2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NNZ7"/>
    <dbReference type="OMA" id="HFRLNDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018450">
        <property type="entry name" value="Romo1/Mgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28525">
        <property type="entry name" value="REACTIVE OXYGEN SPECIES MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28525:SF1">
        <property type="entry name" value="REACTIVE OXYGEN SPECIES MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10247">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01378">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8088334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="134" mass="15269" checksum="48DE8FA1DD308DFF" modified="2022-10-12" version="1">MSGKEDEYEKLLSEQNLKDRLEAPQTETARQRKWRIVKRKFAEVQVYAKQGFIMGSLVGGGFGLVTGLWAAVQYKKLSMIPISVIVSGGSFGFIMACGSMIRSDELIGGEYQQHFKLNNQEVPIEQFWLNRKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPT3</accession>
    <name>A0A8S1NPT3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Heat shock protein 70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0980107</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="VCKPIVT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000071">
        <property type="entry name" value="Cytosol-type hsp70"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1025"/>
            <end position="1049"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8095307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1049" mass="116579" checksum="28B63ECDA3CC2E1B" modified="2022-10-12" version="1">MSKSNETAIGIDLGTTYSCVGVFINDKVEIIANDQGNRTTPSYVAFTENERLIGDAAKNQVARNPQNTVFDAKRLIGRKFNDPTVQKDIKLWPFKVEAGADDKPLIVVKFKGETKKFHPEEISSMVLTKMKEIAEAYLGKQVKNAVITVPAYFNDSQRQATKDAGLICGLNVLRIINEPTAASIAYGLDQKTKGEKNVLIFDLGGGTFDVSLLTIDEGIFEVKATSGDTHLGGEDFDNRLVEYCCAEFQKKKGIDLRSNARALRRLRTQCERAKRILSSANQTTIELDALAENEDFNCSITRAKFEELCLDQFKKCIPPVEQVLKDSGMSKSQIHEVVLVGGSTRIPKVQELLKDYFNGKELNKSINPDEAVAYGAAVQAAILTGTGSQKCENLVLLDVTPLSLGIETAGGVMSVLIPRNTTIPTKKSQVFTTYADNQPGVLIQVFEGERQMTKDCHKLGQFQLDGIAPAPRGVPQIEVTFDIDENGIMNILAEDKASKKNNKITITNDKGRLSKQDIDRLVSEAEKYKADDEKIKLRIEAKNNLESTAYHMKNTMNDEQFKDKFTSDEKRQLSDLVEQTQKWLDSHQNEEADVYKDKLKELESKFHPIMQRVYAQTGAAAGAGASMPNMNRGAGSEFKPTVDXEYCCAEFQKKKGIDLRSNARALRRLRTQCERAKRILSSANQTTIELDALAENEDFNCSITRAKFEELCLDQFKKCIPPVEQVLKDSGMSKSQIHEVVLVGGSTRIPKVQELLKDYFNGKELNKSINPDEAVAYGAAVQAAILTGTGSQKCENLVLLDVTPLSLGIETAGGVMSVLIPRNTTIPTKKSQVFTTYADNQPGVLIQVFEGERQMTKDCHKLGQFQLDGIAPAPRGVPQIEVTFDIDENGIMNILAEDKASKKNNKITITNDKGRLSKQDIDRLVSEAEKYKADDEKIKLRIEAKNNLESTAYHMKNTMNDEQFKDKFTSDEKRQLSDLVEQTQKWLDSHQNEEADVYKDKLKELESKFHPIMQRVYAQTGAAAGAGASMPNMNRGAGSEFKPTVDQVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTG2</accession>
    <name>A0A8S1NTG2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0930137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTG2"/>
    <dbReference type="OMA" id="YEQVMNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="51"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8093511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="317" mass="37908" checksum="01A690863FBECB99" modified="2022-10-12" version="1">MQKRDKSPIIHVNLMKENIHTKPHSLNKNNISSKASLNLVKVLATRRSDMNSLWEPPEDSKQHTNRFDNYFQRHSTRRSNVQTSRERERDFSDSPQQSPQHHQCIRHINKTQQRKRIAQFICCLTKCDTHLLCRECKYKIFQCKHDQIVKLTEFIEQPILIFLKKDNSQSELIGNLILQQMQLFNKVKYQAEQSKQQLQEYLENIQDQLKMKCQVIQKEFNEKVDYQLKLIRNDIDQLVQTSLVYKDDFDNRLNQNDDFIESVKLFKQQSEDPYEQVMNQVNQLQQNLNKIPQLNTIVLELKTDITIKDFSYNKLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTV3</accession>
    <name>A0A8S1NTV3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0860013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTV3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035866562" description="H-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="H-type lectin" evidence="2">
        <location>
            <begin position="49"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8090404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="492" mass="56233" checksum="53A6D6663279B2EB" modified="2022-10-12" version="1">MLIFIFPALIQFVLSYILTRYEVGDVTVMTSGGNHILENSRIYPRQIQTAINFQTVFDTPPELFLSPHYYDWSLELPHGFLVKATLITRTGFLLNTLAVAASQLFAIRIKWFAFQDSRISVITFQQNDVQELQTGTGDRTITFSIDHEFKDATNGLISLNGVKHIKSNQIVEVKIEQVQSKSIDVSVSIYSDSQIEYVRFNILLGTDQSLWSSAAQAEIFNPPLYGNQYGSIGYLSRQATFLIPSIYLNQDRLPIFAYRGYKFDKNANVRCYVIYGPQTPNLIYNLFVWDSTTSYAFFDQVSLYFPQPQIKIVQPNCAELFSECDYNGDSIVICNNIPDLLQKGWTNQVKSLMISTQKQFYLFNQINYQGQAQKIIQNQQCMESFSILSVKFQPVASIIKVIFINSNLNNNNCKYVYFYSQCNYQGTVYQIQQGQSLDRSNKIPFEIKSISICPNIIVKLRDPSYFGGGNEAFSSSQACLDGYKFPTYRTPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTZ6</accession>
    <name>A0A8S1NTZ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0860048</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTZ6"/>
    <dbReference type="OMA" id="HKYKQDA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000010">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH myb-type" evidence="5">
        <location>
            <begin position="84"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="84"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="136"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="5">
        <location>
            <begin position="156"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="408"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="413"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8090464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="522" mass="62072" checksum="0227E78281692591" modified="2022-10-12" version="1">MYRSQRLRMKSQNHLSISPPIIKRRLRNKNEYSEIIEPTDKKKQHLIQDIAATKQKSLTNFALTVTPKYAQERDYDDDEDILFHSKGKKRPWSEQEDNLLIKLVQMHGPQKWTFIAEHLPGRIGKQCRERWHNHLNPQIKKSHWGDYEEWILFLSHRVMGNRWAEMAKQLIGRTDNSIKNHWNSAMKKRIPEMEERLKDIRKRGGMSNQELMNSFTSLERQLLQKLLTSQQNGSMSPRSYSPNNIPRRRTNKQQNYLNPNSVNSYISKMMNEIKIDGIESFNMENKMLKKTAKIVDSIFWNEDKVDDIQNNLYEYIQEKVGAHGYDQDEKVDRWIKNFWIMCQDVFTVSVIKDFLSQRPSFYYNYLHKYKQDAANQVSKNLSRIYEQTNNSDEYQLPSLNQFKTPLKEKVDPHQNNNNNNNQHNDTNSPSNLCLEVKYDDHFQHQIESPSKLLNLLTPKHQAQHQSNKKTPNLKSENKSLIQHSQFKDNLSLSKLKFETTPLKKNSAFKFYNKIQLHQQQQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2024-11-27" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZ94</accession>
    <name>A0A8S1NZ94_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0980004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF40">
        <property type="entry name" value="POLY(A) RNA POLYMERASE GLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="1">
        <location>
            <begin position="9"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="351" mass="41350" checksum="62994F775E5095A6" modified="2022-10-12" version="1">MQLKEQDKVLIEILKKNEMTEQKKKKFQNSIKSLVHFLKQLDYDPFKTEIEYCIFGSQLNGFGTKESDVDFTFLTNSYVDERIALRYLRQEIQSVDQNKFKINELVEFARIAVMKIQDQTSEIEIDMCFNNLLGVINTKLLNAYANLNEKVQQGGILLKLWGKKQGIINKNCFSSYAILIMWLHFLQKKYQMPNLQDKQYKNSKQQQTKLMIKRNIEGKKXQNKVNTLIYKIKLLILPKISLIKLIMIHQFIQQAYEDPFCPQIKASAIKLNQPQTSPIFHVDCKFTKKKICTCQNFCSIPFIQQQNQRALQVFLQHELDGLKLEDFKEAQLLHDFYQEKIENTQEVEKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P128</accession>
    <name>A0A8S1P128_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1010013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1067" mass="123369" checksum="6B6DFC1334210BC4" modified="2022-10-12" version="1">MINIQNSQFDKIEYLALKEIYNGFEIRVIQIPIEIHYVNQNQIAYTYEGQVLRMYYINNPNKFSEEIDSFYFQTKILKTLEQIKHLNWKIECIKESNQIGKWMAIWKNNQTINSGYYFEEKIKQGIWKEAADNYWSKAEISYIGMYQNNKRNGLWKIMLGKKQIGGGQYNEQGIKYGKWIEQSDDFWIENQVTYNGEYIDGKKYGLWNILYRQKYIGGGVYDKNGLKNGNWVDLCDGFCDGCQFIFTGKYNNGLKCGIWNSIIWHPWRQQFDYIGGGQYDVFGMKDGNWVELKSGYQSERFVTYNGNYKNGRKYGRWDTKYKGEFIGGGNYYDNEMKNGKWIEISDSFMMDSQIIYKGEYKNSQKFGKWNSMFRFLGEQNFLNCGGGSYDENGQKTGSWIELSDNFSKFIQITFEGEYLNGKKIQKWSTLNINLQEDKIKKIGGGLYDKNGMKDGKWVEMVGNFLEYKEIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIMGIIRMAENMVDGTLNIKGNSCKEHINNQSGGGNYYDNEMKNGKWIEISDSFMMDSQIIYKGEYKNSQKFGKWNSMFRFLGEQNFLNCGGGSYDENGQKTGSWIELSDNFSKFIQITFEGEYLNGKKIQKWSTLNINLQEDKIKKIGGGLYDKNGMKDGKWVEMVGNFLEYKEITCNGKYNEGRKQGRWDYKIKNKEYIGGGYYNQNGKKNGKWVDLGDYFKEYLQIIWFGEYDNGEKCGKWEYMSRTDQKQAFVKGREEFYEIANKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P228</accession>
    <name>A0A8S1P228_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LisH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1030104</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P228"/>
    <dbReference type="OMA" id="KLMDFHW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045183">
        <property type="entry name" value="Ebi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846:SF2">
        <property type="entry name" value="F-BOX-LIKE_WD REPEAT-CONTAINING PROTEIN EBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8097183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="455" mass="53963" checksum="0C6EA73042F3266E" modified="2022-10-12" version="1">MIVIKTEELNYLIYTYFLEQGYSHSAFALINEVKIDQLIYRDDVKPGQLINLLEKALLFQQLQDHIQINQYYECKVPQLLIQKHVCQFTEKKQLAEKQERRDREIMIQNALMRSQEFASRNGQTMDLNIELQQDIIQLQDEILLSDWNAQKNILAIYQLNCIKFIQNNQIIQIMPTVVGESFSRVKLMKFDRSSGNNMLIVYENGAAKVIDTTIQIIKMFNIKNSSKLLDFHWNLNWPNVVALIFEKDIEIVDIYKCQTLKYLENVIHFEWRHFDHFIVMDLNYYINLMQIDIDKPQLQLKAELETTLIEFNKRKTLLASFSSTLNIIKIWTLQNDDYVFEITDDQKILQFCWCSSQNANYLITLTSQTLNIWDVEFGQIKSTYEIDFNVINMVSMDLLNDTPQVILIGEKVVLYDLINKKRRVFNCDTPNKVLRINDEEILFIFRSSYIVYKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8G9</accession>
    <name>A0A8S1P8G9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1090112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8G9"/>
    <dbReference type="OMA" id="INWDDFN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="194" mass="22761" checksum="E2BFE9D679C7CDC5" modified="2022-10-12" version="1">MASYVANSVLNDSIRQFKSNQNDSKQKIDWDDFNYPPLIKVIHYNIEEVQPEYRLVVRSLWLSSILIFAYTLLNIIDNSVQAGYGLDGIRILYSFMFLFSFNPIQFFIFYRGYKGVVSDPYLLVLYKWVQIILILCWITFSIIDILGFNGFVALSFLFEFLPFCGVLALFEDIIFLLIVFLSGFALFRIWNIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PB00</accession>
    <name>A0A8S1PB00_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1110117</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8100074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PB00"/>
    <dbReference type="OMA" id="IQPRYSH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="465"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="522"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="563"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="595"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="696"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8100074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1051" mass="124642" checksum="58E3BC8C5CCAB4DC" modified="2022-10-12" version="1">MQDSNYTLKQIKINFVIPQRDFFGVKFSEFILQIFNEILKISKNKNVDGILFLQLIQHSLNYIQQSCIEAQDYLIFLHNIKLIASQIKDLVQSVYQNSKKQQSFKEFADPIQQQINGILSQYYQPCLQNYVKLLNLDQPQGMDMIPRPYVIQWLKILNEQAINQLLIQLCHLLLVNVETVSTHPKRTKFLDQLNHEINLIKIMTVTFQGSVWQKGFQRLQRKLEELSQNTLLQISEWARTQKFKIIIWLLLTIMMNFNGFHTERIFTGRGELTKPDTHHYADDESPENSQVEGFDIMWKKHSLRIFCTATKFMVKLKTAVDHYRLRKITHRIYQIIGDKASDYFYYQAKGLVNQKWTFQSYIKSLLIYNFWVEMQIGVIKPDSKIKLLVDCIILILIVMNIFYIPMQLSFSLQNNAQTVDFLFSTIPSWVFLMEIIINFNTAYYYKGMIHEDRTKIFQHYIKGDFFKDLLVVIPFLISQYNIPYLNFVLLLRITRVNKIFEQIEEVTMIREKFAAPIDVLKLMIFLIFVAHVSGCAWHYIGIQEFFESNTGWLIKYSYSEKDWVSRYVVSLYFGTVTSFTVGFGDIVPQTLVEQVFLIIMVLITSLVFGYTISSIQNIFGQLREKTDQHRNKMAKINSYMKKNRINPVLQMKIRKYFEYFFTLDESPELLMDNLNEDLKLELRTSIYKPIITKCKLFKKFDENLLNQLCSVVQIQKFIPGQMIFQENDQINKAYFIIQGEVDIQINKVSIQQQSEGSLGIREFLLQKHIHYSLKATQFTEIAYILYDDFKNIIKEKQSHQEQYCQMKDQLLYQIEKLKCEICSQFHHFKKCPVVFYSPSTDVIVCDYSDAIIQPRYSHKRKQKIKQSTLVSTQYNLAAAVNYMCDNELLNEGINDTILKKFGYVDYQEKQQTIEDVKLKSLKVIQKLQEHHAHTPKMKRMIYKNLMKKDENKLILNKEESILENMHQEFINFKREDLNNFDLHMEFSHYHQDKNLSKVLSNFSKQQRFKPKEQFFSKTRLGKTKQLSSHNSTQNNLMTNNHSIMKTIFNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCD1</accession>
    <name>A0A8S1PCD1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1120087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCD1"/>
    <dbReference type="OMA" id="SYFVRQS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="129"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="347"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="642" mass="75991" checksum="E46842B324448F91" modified="2022-10-12" version="1">MILGTEVNEFVTNRQQSKIHYLSQENKLLQEYIADLENALKLNKQAMSLALDNKRPLQTKDGSTSLSEQYQNPELFKHLNEENKKLMEALTKQQKVSSQLQTKILLQEQIAEEQTNYYKDLISDLEYKLIELKRNIHDKEYAIQELERMKPIQEKEGQLVKLIEVVTPSEQNLKLHEELENVRGVLQKITNEAQSVSESNNALREVNYHLKREIFKMRIVLRSQVNYQNMKDFVYNEYLEKTDNNLELNREVQNHRGKLEQMHQDLYGTTSLGYGFSPEPQQLKYLSKNERMIYQEKLHKMEMMVKGFKELFEKEKNSNLNISKLYNELVKKYEQIQNINELLIRSNQLKDQKNDQLMAELQFYKKQNQNYMEQLKTRKQFNSIQLNQEQDIAVGIFSPRFNQNNERVNTQPSYFVRQSQQLSNADVKFDDTEPKEDISLQLSNSNQKIQNNKLNQQIVPQIKMKKLQLKQLEINTNDNFEQAPYCQTARADSKPITKNKMQPLKIQYYDMTREECVKFIMSMSSEFFQHFDISNIKKKQLIKQMQDPLSQIKKIKRSYSNPFSYFSNETQVAFSKPPQKSVVAITKNEIQQLLVELTSKKKQQSEQFDLFSVDVSYIDGNKNKKQMLDQDISFISNIDQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PH91</accession>
    <name>A0A8S1PH91_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1170184</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PH91"/>
    <dbReference type="OMA" id="IIQHFRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016212">
        <property type="term" value="F:kynurenine-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051326">
        <property type="entry name" value="Kynurenine-oxoglutarate_AT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807:SF20">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="5">
        <location>
            <begin position="28"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8102211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="263" mass="30604" checksum="BFBA123049605459" modified="2022-10-12" version="1">MKHVSNRMLNYLEPQMYAKFTMLANQKGCVNLGQGFPNFAPPQFLREALSEEALTEQLQYTMTAGHPKLLNSAASFFYKRMGLQINIEKEIVVSSGAQSVLCCLMQGILNPNDEVIVFDPAFDLYRPLIEFSGGKHIGIPIKPKVLNNKEMMLKRYSNGQFIYSNDDEWEIDFTHLEQVINQKTKLIILNSPMNPIGKYIFKFIFEYLRMRNIINQQKSQKNTQKQLFVRMLHIIIQHFRIMNLSYILGVSLIQNQKRNLFVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PJB4</accession>
    <name>A0A8S1PJB4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1180180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HYVDAND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1229"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1417"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1555"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1807"/>
            <end position="1828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1084"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1128"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1180"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1128"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1197"/>
            <end position="1206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8102578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1862" mass="217854" checksum="A31C62DC25067A80" modified="2022-10-12" version="1">MQTMLYGVEERPKFKMDGSFYFILHLINHFQYLSIIVNANQNKHYSDFKLAYSQSFLEFFILNNTLKSSMTSNLAFVIIVISFASQLILILFLMFSTFIRIWTSNLIRRDITGSDNMNSQIQQWVMSFNETLHWYFVLYPVIYLQIAVISFSSLTCNSVSIFPKSECDIGIGTQILAITPLVISYINGQILIYIMRNHRFHEPNSLKRRYSSLLLLNNTVILIEIFSHYIYDFEVAHILKYSMANLFAINQIVDQLTNFPYRDPLRIPSIRLAFVYAWIVMSVTAFKFDLLAEENLFFFIILPLPGLAIVGETLSSLFQNHAILNYNSSIQIEEKHIIIAVDQFYKYHIDYLKDPYAQLQYFQFLMIHRLYCKNNKCYSKVKRFETLNVDQKKDLNILTLSIIKCIFKAAQNWLFSIHNLNADLQFEQLQLQYISFVSEIAQKPLIGYLELRQYQNNKLNNNSMYFVEITNKLADQLKKQITDNQVRQNSKAILVHEQRTQLIEISLTQQWQTQNLYENLLQTFINLVDQKIEHWQNLINGYPSLNPFQQNTQKLCEKIMNLRIALDKYIGVPQEWFDMKSTTTKGISVKKLNHAQLNINVITLKLYSLFYSLVMNDFDRSVYVEQYVKDLTSNDRQKELDIIDNISLYNDSTTIILVSIVKNKGKIVNKNQLALANFFHYVDANDFKESVSSVHNLLPKTMMKGHEYLIDAFIQKGHSEYFIRKISGYYENKKGFIEKCYVKLGNLFEELDDYVITASILKCNISNQIIMVDAEGKIIGISEELYKTISAKVPNINIEFFKEFCHLFLIFPSFLSILNQNINKINELDHQFIDDEEHLFYIPSNLVELNSLFVRESYEKIGNTLQEGLESQNLSSWRSWKSVQKSMNSEHSIGFDRGNTTILGEKIPIADKSNINFHFQFMNQHKDQLEKYTIIRTKIKMSYQMMKVKRYCYPYFIIELEHMNEINPQHKFYNRYPTMGQNFEASTTMFTSALNHKSLNETVPSSADKSIYDNKPVSFQPDKAVFESSNNQPINKSSKINEEKIKQLIQQDDSNILFGNDHSQIRLFDKPSAIEIQDVSAPPSEIMLPQNPYRPHFSGIPLQVDQDNDSDEFFQLHKQFDEKSYKVEEKQLQKEKSSDDFKDKDESQQEIEDNLERHSSNQNFNKKNFIQLLVENKKLQQENQNDNEEEEKKKRSIASSSRTSTSKSPSLLVRSLYQVSTFGGGLKSVIFAILLYLLLQFCLVFVKLSIIQNNYDILQTNINYVTYPETLNFYFQKIAFFAWISLQERLDIIQYSEFIKKQQLEELAATRAIMDVKLNDLYQGIIQFEEQIMTDELRLISINDLSFSEQQLDLTQLIQQIQLHSFSYIDNAQEGVIFSDSMFFRLNVPYVYKFAYKYITLLNENLVYYEDRIINDIVILTMTFISVNSCIILYILYNTFLLTQYERQILMLITRVSHKTAEEIMSKLSDLKTILIEPSQLLWKRVNYFEVNYEQVQQNQQVVNLLFAKSVKTQSVTKSNEISGLKSKIKSKRSYQSNSLAQRIYDLTLSYPSNLIFLFFLWFLAILFIIGGILVTISQVSDIKPTLNLNLQLIRFKLRFDTLIVLGECLKTQQVISDQLKEKFQNINIDMNNQVIIDLFTEQTDGFQDAMRSIYDSLASQSGLLEAQKKELLMYFEDSLCNYMSEEIPFCNIRVSNQNFSVPDSFKTTYGNPWLEDNNFDFLSGGITGCVQEFTKTLNLYFSKEIQSKEMQVKTIQETVSFLKTQIHINQFVEYYLDSGKLLQIIGDNLFEQNQSKLQQATTIMQLYIYITGLGLLIILGSISYFWLKYVSARMKLMRLSLTLIPYEILLEPKTMSSLKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PL95</accession>
    <name>A0A8S1PL95_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1220049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PL95"/>
    <dbReference type="OMA" id="PRVNEQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="303"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8103819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="572" mass="66690" checksum="8A27AAC370BE4E21" modified="2022-10-12" version="1">MKLASPNFHMSQENNAQLLEIGQCLKYTSYMIDSNMILTSALHQTLSSSTITLDQVLPARLENYMMATIEQDQNLPDYISNLSLKEIINQIRYDQILQFAKPLVIEQKEQKQFQIINTQSNKQIGFQQKKTDGLISNQIFNNKINNTRSISPQPMSTIINKTQSKTQSQIPFIQAESQFMNDQFMNLNPNLSNMSSNSILNDTPQSNQQHQLNKWTDKSKEKILFQDNYMIEEETTKQVKPNTIIIKKSEDPQFFQQLKNARVASPTIRQVKGSGLSPEPYNQSKSKSPEVLQVNIANHYKKLESGQNMKQQQQQQQQQQQQQQQQQQQQQALKKLPEQFKKLLTDSSKRQSITKPFDQNNTKFALISSGSTAQQKNTKQIKQFAFQDSLNDNQMKVVQSEKQFTPRVNEQEYVNQDIQMMLSEQQSLPKSKSQNKKQDEKYNDNIHQQHQNFKDQFSFKPQFEQKQHIQEIPQQIIQQQIVESRSIRGISPQNRSIQQQFKQQSTIIQQPPVPPNQQKEPIQIKLDIKQFMNSNKPKDQQRSISPIYNQRQQIGVTNENGRSYFVNLNFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTJ0</accession>
    <name>A0A8S1PTJ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mei2-like C-terminal RNA recognition motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1300073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTJ0"/>
    <dbReference type="OMA" id="NKKWSKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007201">
        <property type="entry name" value="Mei2-like_Rrm_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04059">
        <property type="entry name" value="RRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Mei2-like C-terminal RNA recognition motif" evidence="2">
        <location>
            <begin position="145"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8106342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="268" mass="31636" checksum="9D83E41A93F915FC" modified="2022-10-12" version="1">MKPIVFRIDPNVLSQFVKPTGQKKEETSQISQVQDCLKQLNIDNDEDVDQFTNQQPSQPIIKNRSRTLYSNGSTLPETLSKNSNQQIQEINLSISGSSLGWDEVFSSTMPTSFQMFQFKNKRRYEPQQQTTQFQIQLQNIPKDNRTTLMIKNIPNKYSQPLLLEEIDCNNKNTYNFFYLPIDFTNKCNVGYAFINFYDSLDIPKFYLEFHNKKWSKFNSEKICQITYARIQGVEELQGHFQYSTIMQEKDRRLKPIFKQSSEQKLKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUI3</accession>
    <name>A0A8S1PUI3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1300036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="VTISMWV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42535:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_146, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42535">
        <property type="entry name" value="OOKINETE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="239" mass="27036" checksum="C5B67BC4E47FF79B" modified="2022-10-12" version="1">MLNLIFILVSGIHSEEPEVCYKLEGHLCVNNQIVDMTFVPLDSIIGLWRFDKNQMDDSQLLNHISQPCELGPGRGAVGNSAYYSGEQYSIIPHHDQYHEIVTISMWIYPLSSQQSFTTILRKALKSTEYTPTILLWPFHDEANVGGGQIEVIVSTSYDKENLRSKGSVTGRKWNHLAIVLQGLSIDLYINGIHDNVLSLKARPLKNEGPFYVGGDPWFNGPLLYLDDLTFXTMFQNNHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PW71</accession>
    <name>A0A8S1PW71_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1330127</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PW71"/>
    <dbReference type="OMA" id="MYKRGEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053114">
        <property type="entry name" value="ATXR5/ATXR6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48442">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48442:SF1">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="4">
        <location>
            <begin position="243"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8107356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="378" mass="44041" checksum="D43C253B8EB27D7A" modified="2022-10-12" version="1">MSTQSIKEQSNTQPISIFNPQEDKKLELQKFKKNKVCAECKSWIIRQKSIRCGICEDVYHLFCMRPKLTRKPKKWTCTRCLELKNTFPDTNDLEIDTLCGRCQKHVQSDDEEFCSKCSKVFHSKCYGLKKVSPICIFCEQSKPPKIKQNKNLVQFLPLKISLKKQKSFILPCCIYDDSLREKCFNSIQYALYCQNICFNDDLVYESVQKIENNVCYEKLEPLIGKDLETFKKYKQVTKHGYYAPVIVEYNIDQGFYVKAVQPIANNTLICEYAGEVFRFADQVYSTSDSMMSLLETNFAATSLVIIPQKYGNLAKYLSGINNTKKNSKKQQQNVKSQRFNVEGESRVILYACREIRTGEVLYYDYNEGGFNYNTRFFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PWZ0</accession>
    <name>A0A8S1PWZ0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1320123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8107053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PWZ0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="16227" checksum="FEA65D2F8CC630BC" modified="2022-10-12" version="1">MDNALNSLLIRPQNKQIKDLNIKYGQQNEKYPVFQTQFNCLYKCQILTEMQGECLEFRSTNHKTYNKSPLVRQKHTKEQNVKQLPSLTQQIHNKFVVQKQKELVQLLTISFTILVFIVLMLEYYQQKKRMGFYDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1H2</accession>
    <name>A0A8S1Q1H2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1400141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1H2"/>
    <dbReference type="OMA" id="FMNDSQS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="308" mass="35873" checksum="17F77C35A8B423D2" modified="2022-10-12" version="1">MKKISSIVDYPPFEFTGKRIPITALICNEPSLKKETKPNYNFLIFSNQCGLKRSKSTPPSLRELLTDTFTINLTERTLPSKKISILLPFKNAVEKIRSNNREIITRRKSCYGSLFGVNQQKQMINFPTSIEMHKKINGKEFALSSFAEILKKNQRKSTKRPSLARKLSKQMSLIEYDNMERPPSCKTPRETKIISFKKKNSTTLIKEKYIQEQKLKLFHFMNDSQSRKIQLNSLPFRNSIKPRGDTMKLKTCMTELLSTQRSPTSSYRTTTYSRPYQKQNELLSKLIKTEHKSRSHYFPKSTINAFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q367</accession>
    <name>A0A8S1Q367_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1430004</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000147">
        <property type="protein sequence ID" value="CAD8110079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q367"/>
    <dbReference type="OMA" id="HITLIRC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040283">
        <property type="entry name" value="DDB_G0292058-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31414">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN DDB_G0292058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31414:SF18">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035778304" description="Transmembrane protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="712"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="308"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8110079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="802" mass="91289" checksum="D0C5C5C4D1432378" modified="2022-10-12" version="1">MKTKTSITCVFLLCLGCLLVLQKYLDGSETYLENLSQVTVDSCLYVDYQPSLKEISEAKRLRDQLLSTNYRIGLDNLAENDGKFSSEKQQDASKAVIIMLLPWAILLVISFFTHITLIRCWRDQQNKNLSSCLCGFCKCTKAEEIKDIRIPLAISIIFGVIIITFCIAGLIMSDQVGNSIKSIRCAGLMILNDINDGATFNHDEEIQRWKGLDSVINSITIIKNGLCGFVSDTQNQLDLINVQQLQKEYEKLSEVNQNLETIPLTTYYGTNNILGYISTNKKISADEIVLDVQQIKVLPQSQIFQQIYENIINEIANIQKDGQQLSDDKDKISNNLENAKKQIEDTQLNLFNMANDFLDNTKTLYDLSDLASLVFYSIFGAFGFCSLVIVFSSILLCFGKGLKCLRCLLICACSFNFLFVIIGFILSACLGVTSVMIAEVCDYIDVIQTNQTKFENLNYVIKDKQIKEIMTECLFKDGDILNYYNVYDSIKLAIDLDTSLNLFDQQKDELKKQLAIGNASLKINLDFITSFITFERVDESEQIPKQDSIKYMMRQQIKACNNYKKSSVPDKMSYCDASAIQYFPLSEQPFSNPNNQPVCLQSAIFSSDQTLLNQQISDCPEISTINQFYKNQSSSWKPALAQEKSILNKHKQNQDELNVKLDAITQYNITASNYLKSIIDKNTGALSEVNCKFVQRSIARVQNVICAQSFRLFFYFWIFFAIISISIWLSAISIFKVSIGIYYLTTNDTSSPSEYPNRQQIYGSIPIQSNIYEQSGFNQQTYFNQTQIYTNPYIQKQIDYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q541</accession>
    <name>A0A8S1Q541_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1440001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q541"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="25680" checksum="F002D94BBD223BC9" modified="2022-10-12" version="1">MRNFILESLLNVVEMISINQLINEFNQSEFTLMINILFRRSQDENLLIKLLNSSLIYSNEGYSQIETEISCLIPKLIYIDNITVAQEQQATKEQYFYTLLYLRFNFCGLKFLVIYQMIVKYAQLNQYFNTQLPYNLYTYFEHFGLAYLNFISKIFNYLKFIDQIQNSQKLKKISTKVVNDYLTPYLLYNLKTNIIVQISILASIQQQNYFNIIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8R6</accession>
    <name>A0A8S1Q8R6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1500013</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8R6"/>
    <dbReference type="OMA" id="TAFTFFH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF147">
        <property type="entry name" value="PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="162"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8112056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="323" mass="38548" checksum="E53DE9B4C43D0D04" modified="2022-10-12" version="1">MKYYKQGQLLCYIGTWMPFVILVLIFVFFYGVYIQTYLLPRIRQEYIERPVVEINTYLIQLVHINDYVFSNSTVVITILLHTILVLFIITLIRVVITLPGRVPKEWLNKIEGEINKMIENEENMINFHKKGSQTSTSFSSEIDDEQRLQFNTKAKLELIDKSGHRFCKNCQTFKPKRSHHCRQCKTCWLKMDHHCQWLNNCIGYNNYKLFINLLFYSWLLISFIMITYSRCYYDTLNSFQSDSKLFLVSFTFLYCCFLWILLTAFTLFHLWAIKSNITTLEYCENKPRQPFQKSALENFIEVFGINPLIWFLPIQPNTKPILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9G3</accession>
    <name>A0A8S1Q9G3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1500116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9G3"/>
    <dbReference type="OMA" id="EYIQSAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8112262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="52290" checksum="9AB70051B8AC6647" modified="2022-10-12" version="1">MKYYLSQSNNTYDLKLNSEYQFKILCNTINIIIDNMDIQDYNYNQTQVEVLTCSLKFDFKKSIYNFNTIHAKLLYTNSINRILSENDRSILYDITLNEYIVLNNNEESQQEYIQSAQDSFSLIPISIVLNLFNYLWTVLEILSWINNFYFLNVNYPFNVELDFSSSNCSNLIGFPTYQGLNQTDCNYYFEAPKRFKDIGIYLVFINIQVPFMFLLSSIIIFLINYLLYSFFSLLNDALKIKIPIKKEKHFSIFNISIKKQNSQSIMTTQNPYLKYITNLLIQNSVHLLNQFKQTISLFLFDITLAILLQLSYSNNNSNIIVGLNQFFFQIFSFKLKQTLLGDIWIYRIIDFIIFIRKTQILKNSFGYNYQFFGQINKIFQIFFMVNPLMYVTLCQISSFFVSFTYYITIPIISRRNIQWSQYQIFVQVPLYKQLFHLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QAA2</accession>
    <name>A0A8S1QAA2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1520087</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QAA2"/>
    <dbReference type="OMA" id="WDIYAED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000057">
        <property type="entry name" value="Cyclin N-terminal domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="4">
        <location>
            <begin position="306"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8112679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="575" mass="67794" checksum="C98C053DEEE204D6" modified="2022-10-12" version="1">MQKKQLQINGLNHPKCLTQSRSPKYQQKPIEPQMIKQITQLIKSSKDRHTERQYKPIEKLKSIQINQPQITSRATESRDMSTTFHQLLQEFRNNNKQNKENSFNNINKPSQKVRQISFPNTVLLSNTNSRTRIKDSMYLNQCDQNEKLTQKILSIAQTTQIQNNYKLEFNGVIEDMYRCSQESQNLLNEIKDNLKNQESHFRKSSQRCVSADIQMISKSPFSKSRSSSIDHKESRFFGSTQANSNPKELDLTCDENEIVINNINIEDRIKTEPDPLIQLEREYLTDPYYLTEQSEINQLMRAILFDWMMEVAMEFRLKRQTFHLSIFYLDSFLSKKQANKQNLQLIGLTSLLIANKVEEVIPIGVQQFEKAANYGYTKDEILNMELNILFTLKWHVNPPSYTYWINWFTDQWDIYAEDYGLNIQFRKPNEESYQLFRKLCQLVDCTLMDIQTLQYMPRTIVASFMYLIISFQLNVYDQDMLETMSQTSMFLLNKDNQFNIIFGQFVQTTFGFALQDILPAIQYVVGFYELNINYDTPPGVVHLSNTPLESNYEEFLSFQCYSKSLLEFIKQKSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QAN9</accession>
    <name>A0A8S1QAN9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1490061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QAN9"/>
    <dbReference type="OMA" id="YVWEEEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00915">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="1">
        <location>
            <begin position="2"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="184" mass="21751" checksum="CF5BB55D98FCFE24" modified="2022-10-12" version="1">MEGKYLQVGKPTGDDFDDGMIDKISRIKIQYNKQYIVAIGIFYEQRQIYKFHNFCVNSISKFYFYVWEEEFIIEKDDYLQQVSGTYDGTYITSLQFLTYNGQSMHFKASCLGDQFFIENLGDTFSSCSGSFDRNGLTSLVFKLMPLNWNDFERVQNKILLLSYGHHYPQKYVPQFSQYLNYPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBY7</accession>
    <name>A0A8S1QBY7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1540076</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBY7"/>
    <dbReference type="OMA" id="LQQWESF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051510">
        <property type="entry name" value="SKI8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44090:SF1">
        <property type="entry name" value="SUPERKILLER COMPLEX PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44090">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="366"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8113182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1160" mass="136936" checksum="AF135090024B8002" modified="2022-10-12" version="1">MDEILSQISNQVYEFDTLGLVQQYIFELAEHFKENHNAQVDIQQFVVFFINKTDEQGIEGLILISKLIELYQEILSQQNEKILKLKHITDYLISSQEICSEYFNAKFLPSQNNKIQLTRRNLTDIEISPPEILGLNPSMLRRLQTQLKIENIHMNNQIIYGIQGENDLFLILQFCESVCMLHEFQFKKMLQPRNMIDGNFVVYGYYSIRQQRLGVVLRNNTLIFWDKRDNYTYDYQIETQLELQHIAYIEMQDLWITSANKSLQIWNLEQGQYQNLQNQVFSGGIIQLLEVIHLQMLLVASNPNIISCWDMFEQKFLFKFQVSHQILYQVVCSRQFQLIFTIGFNNYISCYELHAQYNDYNLKCQLVGHSSTVQCIEHIEQTALLISIDTKNVIILWDIRSQQQTQTINLQTRILTKQLMFYCNKLCLVANMLQTLSFEKQLHLNKQIKLLQVIYDQWNKRNIIITKADIRIQDIITGKIQYILSDFQNEISASCLIEHGYSIIIGTVEGDLYKVSLKHGQILQQWESFYQEEIIQIQYDEAYKILHIVSIQGILKCLPLTQLNVCKDNIYKDKWAIRQLSVNICINICQLSIAQGLLAISYEDFIQIWNLEYYCVSIQIQLEDNAIVTSMIFCDSLPLLIIGTNQADIFIFQLVQKNDKLQCTLEGHLNIVKAVLIQKLKSVYPDRISIMSNLFSKTSMELIELYDEGDEQIEVKRLFNNKYKLMTHGSFASRLLVQFHKEELKLYIATNRGSALVFNITQLLEKKNVSKKLEQISQRPNYNPYRVVYNKLQGNFIDNYQIVQNRLIKDQVKKRESKFEQSSKIKQKFRNSLIRFSILKGLKDNPELDITHTLKDSIFSFDEQLETVDQIHNDMIIQLNFLYVDPYEILFHKKTQLLISACIDDTIKVFSTDFIQICQFSIHNPTPYQWQSLLNEKNQLKATILFSLELLQQILPQLNSEKRKAYDIENMLRQNSIKHLNKNYKLTNILTKKEPIGNQQKLKDSFLNKRKISLCNNVKMPQLSPKLPPSLRYYDEKIKQSKYQQEIHEQELINQQNQQKRQQGIQIEFSRQQKKEIIDYLTPESNNNQKFFEDLSIQALSSRLKTDTRTHQSKITKTTNDTKRTFTRQSLDFFDYAEKKEIYLNLDFKQEDNIMIFTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNP1</accession>
    <name>A0A8S1QNP1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1820003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000191">
        <property type="protein sequence ID" value="CAD8116821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNP1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="95" mass="11179" checksum="E5FCD883F379C184" modified="2022-10-12" version="1">MNPEYQRIKFIFSSLDKFIIMDFNKKIALQDLKNTFTNVLKIDLFSKQPQFFISKGFHSIQLNSHQSLNGQTIQNFNEKDQNPLEINVKFHEQGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QP33</accession>
    <name>A0A8S1QP33_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1130138</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2030015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000212">
        <property type="protein sequence ID" value="CAD8117563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QP33"/>
    <dbReference type="OMA" id="YIVMELN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8100865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="97" mass="11549" checksum="13ABB8C813851FD5" modified="2022-10-12" version="1">MQEYERVKFIFTVLEKFIVMDLNSKIALDDLKFTFTNILKIDLFAKRLQFSITKGLKQYSLNSQQSLIQQENLRIFNLNNQERKQIDINVKFIEEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPQ7</accession>
    <name>A0A8S1QPQ7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1820011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000191">
        <property type="protein sequence ID" value="CAD8116835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPQ7"/>
    <dbReference type="OMA" id="KVIFGRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001878">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="257"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="56755" checksum="BA1BA025F9BC4C6B" modified="2022-10-12" version="1">MKNCNIPDHETDVEFLCTNEQCKDFRVFCFKCLQNEKHNTHIKDVQKIKDFGRQQINKSDEDLIFDLEKMIDHIYQLFFTLKEELKFGCQISEEELNGLNLNQLNDLLCSKIKFRKEKQSLISMISKSAEYMIETIYTGLNEFSQQQFDRPGKKVKQEIKTLKYELIDTIQDEQINSFAFNQDASLMVGGYESSKIQVFEFKKGQLKMIQQLNFHKKSIYCQYFMKKSDGFLSGSCDKTISICYLNDNQKWDIKQRLEGHQGGINCLLMNRNEDLLISSSDDMLIKFWIKDNDLWECSQTIGGHSSFVSSISLNTTENQLVSCSKDSQILIHQYDQEQKMWIVFQIIKIKQWGRRLCFINDQILVFQPEKREQMHIYQLDSIDKYFVKTKEVIVGVGSERKKNRGCFWFFPQQYLQQRQIVLNKNGCCVNLIKKDENDEFATIQSIDFQTKVIFGRMTDDGEYLITWDDRSGSIQIRKYVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QRV7</accession>
    <name>A0A8S1QRV7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T2410003</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000250">
        <property type="protein sequence ID" value="CAD8118466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QRV7"/>
    <dbReference type="OMA" id="EDCNRCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8118466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="405" mass="46743" checksum="AF709CCE2FB76DB0" modified="2022-10-12" version="1">MFNNFNEGCLEQIDEKCLICQEGWIQDEFLENCHPICGDGIIQGQEQCDDGNLISTDLCYLCKYSCIQFCSICQFGICLQCQDGFIINAKFNCDPLCGDGNLIPYSTEQCELTVNGGQDNCLECRFIPIEYCKTSYFSFCLECEVGFYIYENKCFPYCGDKFILEQYEDCDEDNQQPHDGCFNCKFQCIEDCNRCEKGQCIQKCEDGYEFVNNICLSVCGDQIITKEEDCDDGNLQPYDGCFECKFQCIEDCNRCEKGQCIQKCEDGYEFVNNICLSVCGDQIITKEEDCDDGNLQPYDGCFNCKYSCPENCFDCYQGTCLECNYQYQLLISNQCKKQLTCGDGYLQVQEDCDDGNYQAVQAYKYLFVNMLEQQFQNQSKWKLHKILLGKVRFIICNMIKQMIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QUF7</accession>
    <name>A0A8S1QUF7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2500003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000259">
        <property type="protein sequence ID" value="CAD8118565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QUF7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8118565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="120" mass="14539" checksum="08493C5082B73FE4" modified="2022-10-12" version="1">MASFIKPHFDQVAPLMKKYYFKEQEIYGNSSISIRQILKLTKSISQQRVQAYSHYGGSYRFKDQLKGLFLRESKTIQIIEQEKYDLEMILVNSIYFLISLKYLSNFDHLYRHKFRWRVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMI1</accession>
    <name>A0A8S1JMI1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMI1"/>
    <dbReference type="OMA" id="THPINCL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="164" mass="19353" checksum="920838C6B60B09ED" modified="2022-10-12" version="1">MSSQINDVLRNETIYFRKSHLLRKATNQQNKLFRKGLIQDSLNEPTHPINCLIKNEEVFVPYINERNSLGRLIKKPNLSSNREITNKQLVKSSLKLPKNKNNYAYLQNEPPNSNRSYGQKTKTQSTADSFYSFFTFNKYNQNFLLKSKNSEPFSFRKSFYQKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JML3</accession>
    <name>A0A8S1JML3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JML3"/>
    <dbReference type="OMA" id="NFCKCKE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="52471" checksum="87F6173D365B6D56" modified="2022-10-12" version="1">MSETSLGNKNNNYDEQYYEPGLMESSKFQYEKQLEQQQRQNQQLSSFVPPNQYYNGDYIQQEEQKPLNRTYPFVFTAQNMPKINCFNHQGQTFTNFCKCKECILPLCPECVKEHVYEHSQFNSYPKLECLENILTDVHQEVCQQANQLACAKQDIEKSQTQASAYTQETVQKLKEAKQRILQIVEQYFHSLEIELNNRQKKNLDNFNRDGKSLIDILDARFKSHQNFLDKLQKPDCMYSLLPYLLSTTKEDNYQCLQTAQYYCSRFKEASSIITFDSIKSSQLSLQIAQIVNVIHKDLPEFLDIHKLASPEIIQSQALTVKSQNCQVSQINSYQNSKIQENIQVKQQHESLIIQQQPGLEQSYGYQQIQKLPLQQVPNQQIQNPLIYNGNLQQIYGQQQPFKQTQLIQNNKYQGYPQMLQPYQSIQSTYPNLNAQYINQGYTSQRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRL0</accession>
    <name>A0A8S1JRL0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRL0"/>
    <dbReference type="OMA" id="KCIYNNS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="250" mass="29462" checksum="A1F0214A4562B93A" modified="2022-10-12" version="1">MKLHSETLFQYFQIDHCDLASRIKSIKCQLLTTGIWGQSKHGKNVDLNKLIQGICSFDSSLSFIYKLTKCIYNNSLNQDTNLAIFKDCIQLPYQLINTTYDPFTQTLPKEDEFVDAERFQQVENDINDILDLQEQDLTKINERLSEDDQPKELGNINEKIKQNYINKMKKVNQLIDQLKGKKQVLEQIQQQNEVNDDPRYTLIFPTEYINILQEYNQLQNNVIQNNPIPDLKQIKDQTKTLIDQEFYFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRN8</accession>
    <name>A0A8S1JRN8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleoside diphosphate kinase</fullName>
            <ecNumber evidence="7">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0040434</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 7">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRN8"/>
    <dbReference type="OMA" id="HIVDCAP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04412">
        <property type="entry name" value="NDPk7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000004">
        <property type="entry name" value="Nucleoside diphosphate kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006602">
        <property type="entry name" value="DM10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057579">
        <property type="entry name" value="DM10_NDK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011410">
        <property type="entry name" value="NDPK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037993">
        <property type="entry name" value="NDPk7B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43109">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43109:SF2">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25364">
        <property type="entry name" value="PH_NDK7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036503">
        <property type="entry name" value="NDK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00676">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51336">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DM10" evidence="8">
        <location>
            <begin position="4"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="6">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="6">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8043210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="376" mass="42954" checksum="B5A613906B4E45ED" modified="2022-10-12" version="1">MAVNDERYVFIVEWFDTSASLIRSYNLIYFMSDKTIEMFDLKNKRIFLKRCEYPSVQLKDLYVGSIVTVYSRQLKIVDYADVFTRSKFEVQRGKTFGMIKPDAYTHIGKIITAVERNGFVIGNLRMTRMSIADAQQFYAEHRGKPFFDELTQFICSDFIVGLELIADNSVKKWRDLIGPTKCQVARVEAPNSMRALYGTEGVRNACHGSDAPGSAQRELDFFFSDKSNLKSTAVFNNCTCAIIKPHIVLEGRAGQIIDIILSEGFEISAMQMFYLDRATSEEFFEVYKGVLPEFQAMSEHLTSGPCIAMEIRQENAVKSFRDLCGPHDPEIAKTLRPQTIRAKFGIDRVKNAIHCTDLPEDGILEVEYFFNILQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRZ2</accession>
    <name>A0A8S1JRZ2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRZ2"/>
    <dbReference type="OMA" id="FYYKPNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="164" mass="19524" checksum="A14EFDB0E2F85F5D" modified="2022-10-12" version="1">MSQNNNRQLQARPNRKTEQSFDFFKNTKVSTCSYIKRKGDDFIAIPFYYKPNQRNLDYLNKSMYSTSTNHDAYNNKRRCFSSMRNKPLSQYNPLAHRSRLADQSLRISQNNFCQIEIGDRNQRMTKHYVTQYQNHLGNFGETTPCSNPQILAERAKWNHRLKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSD8</accession>
    <name>A0A8S1JSD8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSD8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035904020" description="Insulin-like growth factor binding protein, N-terminal" evidence="1">
        <location>
            <begin position="22"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="55549" checksum="39222794F34C5398" modified="2022-10-12" version="1">MFFTIFIIIQLSLCDWRLIYSNYYSESINSANDWDFKQNCSINQIQGSSIKQCGTNPLEFLRLKDDKTKLEKEFYYPHYQIRIITDVIFFRSLPGADSEFNIFLVDNFGSSQIHQRPYQENDLQTYEKVCSYSCGGGGGGGGCSNVDDQRVEIKTIVLTSIVHNTPNFNISYCFMYQDNDMRIGLRNFLIYVKPCHFSCLSCSESTELNCLTCYQGTPLGGKCLCDEANQYVQLLIGCTQECNRDYYLADSKQHCQFDPRIKSELSYFTSNSLTNVNLLPYQPWTFIPDPFYYRNQEMLISCSGIDLIGQFYNYEGIQLELGLNKALKFIRIRVSFYLNSWQTDSQLIITVDSFSRASILKTATNYTYSQSSLLYYNSISCLGLNYDLVRIETVLKTNSYNSIIRFQAVTGLQSETWAFRNVTIDYGLCQNNCTECETYSRCLICDSGYQLYRGTCVSSCPIHSILLTNGTCQDYEDLIEINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JT91</accession>
    <name>A0A8S1JT91_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JT91"/>
    <dbReference type="OMA" id="EKENCCP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="122" mass="14470" checksum="59B2520519022942" modified="2022-10-12" version="1">MQIYPQPQDETDRKFIEIPFNIKDQNQQNKYQSESIETTQKDQLSSQFKSKGLLSSDQKSNSQKKIRYDSTGQPILKKSKAHQITFCNQLCQTYYVESWKEYNSNDIQEEEKENCCPFCTMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JU54</accession>
    <name>A0A8S1JU54_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JU54"/>
    <dbReference type="OMA" id="QAESNFF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="183" mass="21437" checksum="DFB2FD665BCE49E6" modified="2022-10-12" version="1">MNVQLNRMEKSINNRCKLLRKVYTQKKKAPLNNMSFQIRVQNMIKHIQIVQNERSRCQSEYENKFPQMQRLNPHSGPQNIELINLKLNTIRITSKYNNLIIDGTTKINQQRNNSQASLNSNIRLSSSPLRFYNKNKASIQTLILKDTIALKQQKIKIDQQTNVESNLFQNISSWSRKSSDSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZG7</accession>
    <name>A0A8S1JZG7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050221</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZG7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="151" mass="18177" checksum="48EB484686483174" modified="2022-10-12" version="1">MLPFEMEPFFPVLIENKTLFRKIKRRHHSTQPIERKAQQPIDSFYERSFVIQRKELQCRTLEPKKQIFIQYPKTSMSGRENINDEISFEKLIQTFLQVNSCNPSKQSSINKKAFKLISQNKEIQILEEEHKSLQSRFFKEINSYVDNCRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3S6</accession>
    <name>A0A8S1K3S6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3S6"/>
    <dbReference type="OMA" id="FCEEQLI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="339" mass="39861" checksum="CFB750F3D6DB0DA0" modified="2022-10-12" version="1">MQKEEQQFKEKQIQKEIQYYIFQDLENVLCLIGELEQTIHTLQNQDDYKQSYLPIATNKTILQANISNGIHFDQYYQHHKTHKKFLEECKNKARDIFEEVKKKKGINATDPKIQEICDFYLNKNLKAETSFYQLPLLPVSREAQFNQKALEKVIWGTQTVNNQLNALQNSKYQPPTVKQILEKINQRKKYCEEQLIENFGINQQNQPNETNNSKNLREFETEEDKQILNDKTIIQDQNSQVFEHKQQKNQNGMQIEDESTEQNLPKNKLDKDDKSKENTDPNKEQQQENDTNGIQIEDEQDLRNSKATKVQDSKFGITELPKATKGDENDCVEEGVDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3V3</accession>
    <name>A0A8S1K3V3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3V3"/>
    <dbReference type="OMA" id="FMICKNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="168" mass="20096" checksum="ECF52150D96333F4" modified="2022-10-12" version="1">MGWWNGNNDEDDANKNFSTNVNSTFKSSTTTRQCRPDPQDQNFMICKNIVKESSTINGQRNDETKEYEERIPISQNYARRNQNYNQNNPEFDFNQMDEEIGQMFENFTNNFFRNFPSVVFGPMRVDFQNPNYETRTNNNYTNQQRPQQQQSYQSQPKIQKKKDDIYDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K422</accession>
    <name>A0A8S1K422_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K422"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="339" mass="39490" checksum="2753AA2EF2CE5486" modified="2022-10-12" version="1">MILSERDFLNVKGKQFQGVHSNRICSPNESLISVSHNPSLPQIKLQAQNVLAQSIHKDLSGAPPFSQIHLKPLICKNKSCIYLVRNLEDKIAKYIVKIKEIGKENNILRQKLQMDRSFIDNLVPSINRSEQFSQLKQLTEEVQYPRSNFNSLHDAHLIRKFSNEVYHTNLTQKRTFDIFNNLNMLFKQPFSDISWNKPTVMNNQQVQSNKCLKSCQNNAQTDFIELNCNHTYHKNCLCEEVVNSPKVLSSFCQCNQKLSKHDMNQLNEVQRAIIKEIKLTSQLSQLIKKSDKFAFYKCQQSSCPFIIIDLDYENILSVQSFCQSCLNMRLFYKKEQMEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4L7</accession>
    <name>A0A8S1K4L7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arrestin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0140420</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4L7"/>
    <dbReference type="OMA" id="NCKLDVT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin-like N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="3">
        <location>
            <begin position="187"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8050298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="44371" checksum="D91D90C0F414A823" modified="2022-10-12" version="1">MGNQQGSDFGGVYIRTDKPFYFAGEIITGNIYLNIFKDGFPGGVVLLKVSGKEKCHWTESRTRNSTGPDGKQKQTTEYVTYSGETQFYQHRVAIWTFNTPCLPVGQYTFPFQFQLLSHLPGSYFEKGSNYQAKISYSVKAEIESYNKSYKNIQHSQPLIVREPLKQNMTALMGQMEVKASTWCCIDKGTSHIKCVFDKNHYLPGDNAYLWADLDNSNCKLDVTRIEARLINYLTLRDRTGREKLITRTIVSQQMQGIKAGERAVENDRKQIQLILHNKSSPEGIHPSSNGQLVKSVYRLEVQAVLAGCTCCNKHPKVNVPIEIVAPVPQIYNQPMVQPSNWNPQVFQPQIVSFTDSTLYMKDQNQQSNYNYPQIPPQPPNQFGQMQQPMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K846</accession>
    <name>A0A8S1K846_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K846"/>
    <dbReference type="OMA" id="YCIIVNF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="408" mass="48529" checksum="0255ADCF6AD784B3" modified="2022-10-12" version="1">MLSSKTLPEQNFGELAESENSNEKSPKTINSSSFLSFEELQSFILEEIQISYEVIENYGTSKNQFNLLKIINESDVQNQNIYYCIIVNFTDELLKQKIKIDISRYSFQTKFFDFTDWQDQKDSLVLSDSTSTTEPQVFKDYVSINSQFCRIIVFSQEFDQKIIDLINNYPYIYYCSDVKIVKEEYLESLKLHQHFQQIEINYKMIKHLISTSLQRLKDIKFAGIQIKGCIEYVDKIDQIKIHPLIDYQRIGKKQIQFKYDIPFIIINSYFYLPIPLSIKNLEIKDHLTQQFQSKPVHISSNINHIEEYDKIYILLNSIQDQIKKDELFFSINSNKEFNSLESLLTLNQVNQIQDLKNQLIDEDHYLNLIEKILIYFSQCLKSTIEKYILFVQLKQLILYRLHNSYLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KBS5</accession>
    <name>A0A8S1KBS5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0190278</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KBS5"/>
    <dbReference type="OMA" id="YLCNIIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14811">
        <property type="entry name" value="bZIP_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416:SF8">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="127"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8052629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="354" mass="41860" checksum="AB8B7D0EC8B07AEF" modified="2022-10-12" version="1">MKATYDIIPSDSLIQLINSSYELDQMTNKWMFGEEDHQQASPPHYDQIRKCRPLDKHNESIINNNKDLFNVNYSKSVYVNKINSLIEIAPTCQESSLAIKKEQKTYLKTKKQFEKDNGYYHEEQGFDDSNKIKLAKNRQSARDSRKRKKIYVELLEIKVAELSKQIEVLQKNLEQQNIFIKHCVKIPSLISDFNLSYQNQFQVLSQNITKKQLQTLDDEFGVNSEKRDYLCNIIFNTLVDHILPFDLKKTIETAINNLGIFKLGEQQEIKQLMEFQQQFIQNYKKFELAAFDMAKSYEYIKREAEKLEKLKHQMVSLLGPQKYVKNILDIQQLQQQIIVKEEDINQKSYTKYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDS1</accession>
    <name>A0A8S1KDS1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDS1"/>
    <dbReference type="OMA" id="HMAYNIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="69" mass="8076" checksum="4A74308AE2454CE7" modified="2022-10-12" version="1">MGCTAQKDLHKKTDFDEYAQKLDCHDHQFKGMVAFKSLKQKHMVHNLAQLQKTCSPNHQCYQAQEIEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEY6</accession>
    <name>A0A8S1KEY6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mannose-P-dolichol utilization defect 1 protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0210181</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the MPDU1 (TC 2.A.43.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEY6"/>
    <dbReference type="OMA" id="HLQNQAG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016817">
        <property type="entry name" value="MannP-dilichol_defect-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 LEC35 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226:SF2">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023381">
        <property type="entry name" value="MannP-dilichol_defect-1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="96"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR023381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8053899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="206" mass="24301" checksum="77E3DEE155D21104" modified="2022-10-12" version="1">MTFIYNLISYGIVFGAIFNQLPQIYKIYKSKSIQGISFSSLYTETLMLIFNVAYNMHEGTSFLLYGENVILYIEYIIVIFQFGYYNSMQRDYIRKLSFLGIISVVFLFQIVPQIIFKLSIYINMILLFFSKWPQIKMNYQRQSTGQLAFLTHLQNQAGAIPRALSIFAESNNKLLYCLVILDNSLVLIITFQFLIYWKRREIQKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KFM3</accession>
    <name>A0A8S1KFM3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-(apurinic or apyrimidinic site) endonuclease</fullName>
            <ecNumber evidence="8">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0200104</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6 8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6 8">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KFM3"/>
    <dbReference type="OMA" id="WWSYRGR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09087">
        <property type="entry name" value="Ape1-like_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000130">
        <property type="entry name" value="DNA-(apurinic or apyrimidinic site) lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00195">
        <property type="entry name" value="exoDNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF6">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00727">
        <property type="entry name" value="AP_NUCLEASE_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 6 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="10">
        <location>
            <begin position="100"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="7">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="7">
        <location>
            <position position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8052993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="357" mass="41093" checksum="05BEEFE5277BB121" modified="2022-10-12" version="1">MLQIARNLIKHTHLFKISKMPPKITLDSNSNENVQKENKSKSQNQKEGSKVKNEKKSNPKILKQQTLKLVDKKGQIVDLSQKKLDLGDGIFQQDKLKISSWNINGLRASSKKESAVTYLNDKKFDIICLNELKVAQEAFDKENLITKLPKDYFAYLNFCKIKAGYSGVAILSKVKPISIKFDMGIHKHDQEGRIITAEFEKFNLVACYVPNSGDKLDRLNYRTQEWDVDFQNYLDQLKQKKNTIICGDLNVSHTEIDLANPDGNKRSAGFTQEERDSFSKFLSKGWIDTFRHLYPKEIKYSYFSPRFNSRETNKGWRIDYFVVNQEAIGSVIHTDIITTIEGSDHVPIECDIDLTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KK36</accession>
    <name>A0A8S1KK36_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0220179</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the THOC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KK36"/>
    <dbReference type="OMA" id="FGNCQIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019163">
        <property type="entry name" value="THO_Thoc5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09766">
        <property type="entry name" value="FmiP_Thoc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="109"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8054731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="525" mass="62772" checksum="C79E05AF753ACFC7" modified="2022-10-12" version="1">MELQQKIQKLIDQMRDNVSKATGKRNDSIDYQLLTDLRKTHRELYRSIEGNRRTNQLEKDQLDKQTINSECLKYSKTQLQLQIGQFKALQTNHYNQIKKQLDLEDKNPLNNNKEILHKLENELEVRREKKKKYEEITNEFEHLQQHYEQKKSQYFQVLPDLLKQTQSHLIPALNMFEIPHLALPSQQEFYQQLPQPLHQIYQKLQTKFIQLEIISHPIADSIYKQSQFELKVTIESGIFDQIALNYLGIKLESFYENIYPFSFSIRYFTTLDKIVFQVDRKNQITSEELFCGLFQGDPGEPIFVNAKSDEEININTKYMTFRWAKLLTGFELSAQEFYLQLQKRIYNIQLIKIQLQKLQKNELLTNFPGLNFEQKYQLKYFGNCQINDDTPFWYKFCLGESLIYKQNKDTTLLVEDKTLQSYIQLNYRFEGQAEHLHDCYKMVLAKEDQQIEIGIIVPLSYPQQSVRYQILSTQNINISQYEFFIICKNLGNCLIEGLQLSYQILRIIEQLSERRQKMQYFLKHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KM51</accession>
    <name>A0A8S1KM51_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0240029</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8055927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CKWININ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NACHT" evidence="4">
        <location>
            <begin position="100"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="802"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="844"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="886"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="928"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="970"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1013"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1055"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1097"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1139"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1181"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1223"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1265"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1307"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1349"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1391"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1433"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1475"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1517"/>
            <end position="1558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8055927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1748" mass="200438" checksum="783383C787157A84" modified="2022-10-12" version="1">MNKLENLQNTINIIGRDISILKGKSFQQLFDLRKQTVLLNKKRMDLTKIFVPLYGREKSNGLESKLLPMFDDDHQNKISIVNNFIWYDHQGQEYKDQSDVLLIHGQAGSGKSTIARKIEEFLWEQQQYQNNKEQNLIPIFVQLPSLKDPLHSAIEETLQSEQLSFDKIQINQLKEEIQKGKISLIIIMDGYDELKTEYSQQNLYILNRLNEIWKKPKVIYTSRTEILNSNLYQTWFQGNGKLKEVELQSFSSLQQNQYFQSFVFQLVKQKILTFYEYSIQIEKKIFNFEEFEKIWLPINEMIISKSIEGKNYLNLFLNRNQIKSIINSITQNSKIEIRQDHHEELLQNLQKELSEIWNANRYIEIMDAINIRSIVQMPFMLEIVAQIFPKLTSATSDINQIKSNFIKNYVNLKQQQYLIKKYSQNLDQVFDIEIEQLCALNNWKQLEMKSIFQNYSPLSQIKIENQILLINEESFDFGQEAQIIKQSLKNQNYTPYTFYQAFIDYYHNQQVQKLKMIGKAIDQEIFFTDLINFSQSLALELTERQLTQANYIKKSYVDFSNDGRIPQQEWIDKYFGQQGIVAEQKYLLRKCALLNQKGNTISFIHKSIQEFYVSKYILDLLFKLPTLNLEDLNVYINEKLKNQQNKNKFIKLDKEFNNLGKEHIQKMVELIVKIIKESKFNTLNFSEEQYQGMIHFLKCEINQNQKLKQVLMSIVKISSLESIQQEAGNSLFILNQIGDIFNDLNFEHIQIKNSNLNGASFYNTILCESKMDNVNISQCNFNRAQLQNCKWININSNELPTLKGHTDSVSSLTFSSDGQTLASASSDYTIRIWDTKSGKEIMKLLGHTGWVWSIAYSPDGQFIISGSSDNSVRLWDVSFGYQVMKLEGHTGQVYSVQFSPDGQMIASGSSDTSIRLWDPNSGHQIKQLNGHEGWIRSVTFSQLGHLLASGSCDQTIRIWDLKQYQEIRKLEGHSDSVQSVAFTPDSQILASGSMDGSIRLWDIKNGIELQKLMGHNNNWIWSVTFSIDGQILASASNDTIIRIWDVKSGKNIQRLEGHTDCVYCLVYSPDGSILGSASKDQSIRLWDTKSGREMNMLEGHVGSICSVAFSPDGLVFASGGEDQSIRIWDSKSGKELSKLDGHFGWVQSIAFCPKGQLIASGSSDTSVRLWDLESGKEIQKLDGHLSWVYSIAFSVKEDLLASGSEDQSIILWHIKTGKLITKLLGHTNSVQSVAFSRDGLRLASASSDNLIKIWDTKLGQEILELNEHNDSVQCVIYSPNGQILASAGSDYIIQLWDAISGKDIIKLEGHTDAVQSIAFYPDGKVLASGSSDQSIRIWDITTGQEMQKLDGHAGCVYSIAFSPNGEQLISASEDNSILLWNTKSIKEMQQINGDSMWIYSVAQSPDQQLLALACIDFSIRLWDLKSEKERQRLIGHSDYVEVIAFSMNGQIMASAGRDHKIRLWNLKQQIDVQILIAHSATIWSLRFSNDGLRLASGSSDNTIRIWIVKDTEQEKVLRGHTEAIQQVIFTPEGKLLVSISNDNTIRKWNLDSGEEVEQLEVNLGIVWAATFSPDNQVLAMVNKNNTIFLYYVIKGQIKIIKNNLDLLHSITFSQDSEFLISKNSDCSIHLLEIQSGLEIQRFQEQPTLKQSFKNPFLQQFKQSKILLSLQKQLKNRKSVEQFTQSFQVNNFAWSNIRMKLIPITCYKIIAISHLIQAKQTIISNAIIFSNLRIDLIPLFEQKNDQMFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQU1</accession>
    <name>A0A8S1KQU1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0260129</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0260130</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQU1"/>
    <dbReference type="OMA" id="KTNNQAS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="184" mass="21777" checksum="A72106AE28CC303A" modified="2022-10-12" version="1">MKTSNQASNSNNFKLRIFLKSQSKGESSSIYSILDSQKLVFGDDEKNHKKLKPLILYEDISDRQKSTTASSSSSQLKKITKRITHSIDQEINPENPFNFYQIIDNYYQQYINLPLKKDRFGSQEKNIKKIQRLYTEQSEFKQNKQKPIKLHLTQSKYQFKQEFVNVKEKLLNQCKKNMKNIQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRB2</accession>
    <name>A0A8S1KRB2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0250158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRB2"/>
    <dbReference type="OMA" id="QNAHPVP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026306">
        <property type="entry name" value="RSBN1/Dpy-2/CEP530"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354:SF11">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354">
        <property type="entry name" value="ROUND SPERMATID BASIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8056981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1264" mass="146887" checksum="04278F519093976B" modified="2022-10-12" version="1">MMVYPIPSQIRYDYQILFTKMCDQAVFVLKLWISSLNPQMKLYKQCQSLYFFILMLLSLLKNVKNKYFLEQSLLLLKQHQNCYYKSIELARIQSPTLQQIIKGLFQLIIIFYKTDRMRTKHLVLLGVIIMQVCFVQALHYDLTTLLDQQNEVDCENTDFVQMLQDFDSWSEIIELLQGGQLDAEMKQLQELSDFTNEMISANPNDLMEDQQYDEQLEQMDEQLQTIQNQYNGPQRKFLFEQSRQISDQIRRVIESRSFEQKRSQLAMLYGLIEYLLKQITRVQNQYQQTTQPQPRAQSVQQQPQPQQRIKEQKERIIAIKDKCGDRYQQRKVSKQRVVPGNYRQNDHPVPEEVGCDIPFPQQRQPKCVVGSDIILTSSISADQPTVETEVKKLKDVNEYGFGFWMRFLTLYPEQLQSGLTDQSYFVAKLTKNQQDGDDKIGDRLLTIFQTQEQYIFSAQHDKPERKEATAAIVFGDIEAVWTYVYYSYSAYQQQAIGFYKQSNSQLVKQVTLPASQGTPQYLRFVLGGQYFDFPGFNGQISRPVLAIGLGTYLINEQEFLQYAISCNPQPYVAPQKLVPYQFARESTYVDIEDNNAPPTQEFIDLLLPDEYAVQGWFKWEETDLQENWHTMFRLSNTPLRKQQILLGERVLAAWLGKPKGGQIHFSTYSYANMKGSGNPNAHQYVQHQDQHLHWHFVYFGYSRDQRKAYAQVLFKHVHAKSLSFVNVNHFVSPKHYFYFGRERQFPVYSGFMAYLEVFFCKGSYLTNVKPALRPVPTPPPPKKRCVEGPNRIINAKYDKGPVVHVELHKDDLKDTTQYGYGFWFRYTGLAGGQNENSRPDWSLIARLTNKKETPKDIRDGLLTIFQGKVGFFYITANNKAKKLVELAQPFGDIEGVWIYTYFSYTRYKAIAFYQIENQAPITLEAKVTHPRMEQLLFQVGGKDPQDRYYSFNGQFHRPVLRLGSGSFFDTLEEYNPFCLSCNPQPQKDCSSKGLIRAVSAKASQFNGNPVNSGDRFGTTEQYSVQGWFKWNGKTSEKDQLLFRLTSTLAGEDALNFDTLSCYFDTRDQTLNFYTYTYTDQLGSGNPEVRQIVEGKTFVKDWFHIYFAYSRKSRQADVIVEHSQGKGALSFKNVNHYVAPSLILYYGKDQLTDAFQGFIQGLNLFACDITYQPTPKPVEDCTPMKDPICLAGDQYENVSLTNIDNVVQELEKRKQLMQDKQFVLPTTQCFCFPQQGKKVLPQRQEGLLEIYDDDEEPQEQDAIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVY3</accession>
    <name>A0A8S1KVY3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">P-loop containing nucleoside triphosphate hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0250089</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="INVKICV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057373">
        <property type="entry name" value="ZNFX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF341">
        <property type="entry name" value="NFX1-TYPE ZINC FINGER-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25396">
        <property type="entry name" value="ZNFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZNFX1" evidence="3">
        <location>
            <begin position="260"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="1">
        <location>
            <begin position="447"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="1">
        <location>
            <begin position="765"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="2">
        <location>
            <begin position="868"/>
            <end position="1051"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8056856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1597" mass="188983" checksum="94D380071C51457C" modified="2022-10-12" version="1">MSDYDSDIEFEQCFQDDGLSNWIRNCYIKNYNVDKILCRLNQEKQLEKKLEYLHPNHQQFDSYYYLIKQLNQYIRQQQNIDLYLELLIKSQSFQNEILERCHLIITQINSINNIQKKIEELFQIFYFLKDIDMNILKQSNISLIFSLCRIIKMDYPENEFKSLIDQFNNFLRLIQASAKKFQYKNTPVYPISLEFIQFKRENKQIFGELQELDTKEKSLLIYLDQHFNILREDFIYIMRKSISYFSETGFYNYIKSEYLNNIGLYQNINLISFEMNNYDLLWKISLEQFNLDGRKYQVIDWNRSKKLSRGSLICITNVECHPLLFGVITQRSTNQDNYQNQNKINLEFRFLNPKSQIMEFLMLLNQNSILIEYQTQIEPYIYFLEQLKKIEKLPFENLILKNQNKVLHPKYLQQNSLYQIDLSEQNTYYRQKIDVLNESWPVMESTLDNSQLKAIKLILSKEVALIKGPPGTGKTYCGVLAVKILCENLIQNNFPILIVCQTNHALEKFLKSLIQFVSSREIALLGAFPKSAALKDIHFQQRLEIQFNWNQFKELKNQIRIIFNRLSQYDYFINANDIKEFWPELSHKLIHNFMDDNNLDKSQIDVNYILNCWLDGKLPNCSRFYDQVELRGMYKYFKKFEITEQVHTNNIFEKVYESYDDDYYSDEQDEDLDSLDSLDSLDSFDSFIDFEDEYQEDEFDFQQEDEIKINYNKLQLHYNFYGIEEIQKHIQKQTFNPWELNYYDIQEIIQYLEFLKYKKDCLLFEDKYKQFKEMSQTLKNLYDDEEIKKLNQYKIIGVTVSSAARHLSKLQQLNSKVLVIEEAAKVLESQIACILMKNLQHLILIGDHNQLGPELKCYDLKTKYNGNISLFERFYNNKIPQVELTFQRRMKTKFADFIRLIYGKQYQDHTYVEENRNNLDIIGLEHDLLFFNHSWLESQDKKSKENIEEAKMILQMVQYLVKVGYKQQQISVLSLYSRQVNILQKYFIKQSLNQVIVQTVDNYQGEENDIVIISLVRNNQYNQNQLGFLGDQKRINVALSRAKIGLYVFGSFDFIKEVSSNGSLWQKILFLAGSKKCLKDSITLKCLTHSTLRNVKQPNDWSKFDAGCCDKICNLKFEGCNHKCQKKCHLGDHYRDQCPEQCQQTLKCGHACEQKCRDQCICTKQLEILLDCNHMAFIACGKNPAEVVCQEDIKLTLKECQHIVNYKCFQRKNISLKCQYQCEKVLNCGHNCKRLCWEICYSRKGIDECFSNYFKHHKKNSLELKNKNRDLFFCLVKKNAQNLKIVGINVKICVMSDALLVKKNAQKQKIVGINVKICVLNNALLVKKNAQKFKIVGINVKICVMNDAPLVKNDVQKLNIVGINVKVYVMNDALLVNNRFLSNFIVDSIIFIKIALKFKFFQFQLKIQLLIFLNLELVKITQIIYLENQLKVVEIIFKFLNKNMNLFVDLLVKNQENVDTFFNARIYVLKNALHVSRGFQEFQIVVINMKLYAIIQIISQEPSNVKKNAQDSTNVGITKNVSSYVLNNVHHVKKQLQNNLIVDIQQEHNVLKIIFVENNVQEKENVVIKILVLIGVIKNVLLAKLQLKKLWLVDTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWX6</accession>
    <name>A0A8S1KWX6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0250304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWX6"/>
    <dbReference type="OMA" id="ENHFECF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8057176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="105" mass="12767" checksum="12AB8ED22FB0A882" modified="2022-10-12" version="1">MNYQKFRQNVDFYLKSQKEKLESPKEKSFVSPIRIKTQQSNQEQENKKKPIAIQNMMERINKVILDQRVKSPITTQRNVGQKNKENHFECFLKRQSFLTNYYKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KX04</accession>
    <name>A0A8S1KX04_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0270045</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the argonaute family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KX04"/>
    <dbReference type="OMA" id="ILIHYNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04658">
        <property type="entry name" value="Piwi_piwi-like_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAZ" evidence="2">
        <location>
            <begin position="207"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="3">
        <location>
            <begin position="461"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8058103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="764" mass="89396" checksum="E714DC3F16F402CA" modified="2022-10-12" version="1">MQILDQQQTNYKPPLKPPRSQNISKYFQLMKFSSNHYEIKFLKNLQLQLYSITFAKELPQEQQNELIIYATGSSQFIRKNGINRFWISGYNLWSFEYVEKSFQVPGCKQLQNINLIIKHAKTYNLTQNLDQIPPESLKQAMNAILSQIYQERNLKQIFRKGQFFDKDKIEKINDVQILQGFTSVLQIGQTSPLLLIDFKTKIFNKQTVLQFIKDNKKSKNPIDLQQFFENKTFMTIYSKRMYRIKSIDLKSNPTFKMESSNITYEQYYYQRYKIKLSDLTQPLLVHEDRKTKQIIRLIPELLYMIELTNTQKNNFKVMSNIKQQTTLDPLERSSKIDNERTLLDKYFTNHSISLSNNSITPAYKIIKPTLFPEANIKQTDGACFNQRGKLIEQSNINNWILIHYNEEQQIANLFTQEFIKSTKELGNQINAPKKIVMNNLDPAQWSQALLNNFNTNGKPQIVVSILDRHEDSKLYFELKRFLISGQGVLHQNVSIQTVDSKKFSKIAQQIVKQVHSKIGKQLWNIQKITEVSDKIMIIGIDVYHKTLINNKSCVGFNAQFGQQSDKHFTKTLIVEKGKELNKGVGQLLNQSLQQYQKSNKYYPDTIIIFRDGVGNSQIDDLIEIELKSMKSVIQQTYNIKLPQFAYIIVNKRISDKFFSPNNENNGMIFPERITSKEFDFFLIAQQVNQGTPIPTHYTVIENTTNWNEDTFWRFTYFQCFNYRNWSGPVKIPACLQNAHTVAYRVGEVIQNDAHAALESLLYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2R8</accession>
    <name>A0A8S1L2R8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0320160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2R8"/>
    <dbReference type="OMA" id="LCQIRQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="170" mass="20037" checksum="A4C9BC7F402DFE82" modified="2022-10-12" version="1">MTDIDQSIEARIDKFESDLNRVSLQQDALLPLLQMIEYIPSIQRTETNVKQVQKKQEVNEQKQGRLERCAERKLREIESMPQKPQLEVQQVDQRIKDLESSINCLYRWYEAKLKEGSQQIVRSIDDKLGTDPNAEFNDQLLIVVNQTIKNALDKMSEDFREQLCQIRQML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5J1</accession>
    <name>A0A8S1L5J1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphodiesterase</fullName>
            <ecNumber evidence="5">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0260288</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="5">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5J1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="PDEase" evidence="8">
        <location>
            <begin position="277"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8057924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="611" mass="71022" checksum="972A54C8AE30178F" modified="2022-10-12" version="1">MLPSRSDSQQRSLENPDKQTMRVDSTKRTDKLIEVPRKKLNMRLYTESEQKIQDYVNSKIVTISIIGLVGAYAILIFIIVALEELLDETEFNNVSSILSWIELGILIVFILEIFVGLYAWGIKKYYKDKWLILDTLIILLSLFFVIFELADQKTSNVIKVIQAVFRFLRIFLLIRKAQTFRRLATISTISTPAEKIVRFLAELKEVLEDENMKLDIDYCIDKIGNNQLYQMGKLDEDNAEAMAWLNQSQQGKTEVKRILTNEQQQQQQQKKVIANFQKLNIPKRLLDILIKQHDDLYIDPFECDKLCGGEGLVNLMLFFFENYNLFDVFLIKPKVVRGYFEEVMKGYLDNPYHNKVHAQDVAQTCNFLLSKCKFIEIGQLDEQDIACVLIAGAIHDYGHPGLTNAFLINSRNELALTYNDQSVLEMYHPSQCFKIAWGNQKVNIFENLQFHKYRRIRESIILMVLSTDMAHHASELAITNSRIAAPDFEPNGKDKQRLMDLIVHSSDVSNPTKSYEIYKQWTEKVLTEFWQQGDKERDLGLPISYLCNRYTTNMAEAQVGFIDYVVKPTFACVLGFLPAYEPFFQNLDKNKAKWQELIEYYKQQLASIQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L8N5</accession>
    <name>A0A8S1L8N5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0330269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L8N5"/>
    <dbReference type="OMA" id="MVKREYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="69"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="199"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8062665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="482" mass="57035" checksum="931E411ECB7C0D27" modified="2022-10-12" version="1">MIKRLKFNTEQIRRASSVLQKIDLRMSSKDFSNYQDIIDIMIFILLKPCELRSLADVELLKQATRSLDFFANLGSDLLHEQCCRNLRHKYVPIGYFIFKEGEQGDNFYFIISGRVQVLKDFNPSDPQASIFDNEIKQLSNFETFGERAMDSDALRTASVRAIENTHLAYLNKDSYQIIAKSVKNQLKKAYFEEFANLDFFHNWRFQDIKVFYDRAYVKKYSMNATVYKEGDPLDYVYIIKKGEFKIVKIIKTSTIDLNEMFKGDFEKILCGMSTPRTYTISERLEARFQRKKYGNLYYMEKKKPVTIKYITTGQMFGEMEFLMQNNNTRTHSVYSVTEQSELYMVKREYFETILDKVPSIKNALIQLSEQKNNNFMRQLLQYEKHFNDLTEVKKDLERNINLDFLQLGSTEKRLPSPVLQQIRSTQPVVKKVVKIPKIEREVQRIETKKKFLMRKRKKSAFKIHYSNSEMDGSQYNFISSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCM6</accession>
    <name>A0A8S1LCM6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0380091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCM6"/>
    <dbReference type="OMA" id="LEHTYDP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="478"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8065798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="611" mass="72145" checksum="90F80ED36992A45E" modified="2022-10-12" version="1">MQNNILFSNGQDEAILSERNEQEQFQSDLSNEDEDSNDQIENEEEQEMNPKDSQTPTFPEQQIIKDETKKKDIKETCLEKIKAYIGFGNRDLFINEKIGSIFFLPISSNWTLKIQVTIAQIIIAITSLSLFIGVQFIGQKILFDTVQQWTNNNVSELLQKQICEITIDFVQLLINTRENEIQLVNVTLDLIQSETLRYNGLLYKMDDQLPNSTRMYLPNISIIDQYTLNHVTYSLLSEQQISEKKIRNYLSINNIMYYNYQSMGSTFFWIFDDGFMMQYPGVNVTDVKIYNEYRIQMYKSRRYISHIPLEHTYDPILNEILQREVLPITDINNKQIGLIFCERKPFTLYMLFYSIATNNQYNYTLFLFTEQNDIIYYQKQEYEINIEEFQQVIKTMNTDDDSKLKKINLSKYLVNSESVEQYIHFYQGDLSGQQFLFAYVNYSGLLVGIFQPSYLPNEDIELQKNIYQKKIDNFNKKLIPIALSIPIVYIIICVFYMVRYIKPLQRITEYADQLLKKSQNFDEKQFEKQFNDSTGDDLIQQLTSLFAKLMGNLNKSKQLKKQEIIEFYNKQTYPKNQKSRDVTPIINEVKKIKLDSIVRDPGVLQFPNLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LI61</accession>
    <name>A0A8S1LI61_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0370114</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LI61"/>
    <dbReference type="OMA" id="WKAADPP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001779">
        <property type="entry name" value="Probable myosin light chain kinase DDB_G0292624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="11"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8065133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="314" mass="35667" checksum="077023148D2DDBC4" modified="2022-10-12" version="1">MQGHEDITKVYTFEKTLGEGAFGVVKRAKKKSNGDMYAVKIINKDNLSNEDLQALQTEVEILTQIDHPNVVKLYEIYEDDTNFYMVMELMTGGELFERIVEKDHFSEKEAAATLRPIIDALSYCHKMGIAHRDLKPENLLFSSREPGALLKVSDFGLARFVANDEVMMTQCGTPGYVAPEILSGHGYSEAIDFWSVGVILYIMLCGFPPFYDEDNDKLFKIIKTGQFSFPSPYWDSISNEAKDLIKGLLTVDPSKRFGTEKIMKHPWLVNNTAKSIPNIQNKMKEFYGSATMKKMGNFVKLAQRWGKLSQIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LRE7</accession>
    <name>A0A8S1LRE7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0450137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LRE7"/>
    <dbReference type="OMA" id="RQICVIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05379">
        <property type="entry name" value="CAP_bacterial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31157">
        <property type="entry name" value="SCP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31157:SF1">
        <property type="entry name" value="SCP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="14"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8070257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="278" mass="31213" checksum="FF59E8975C5E4CDE" modified="2022-10-12" version="1">MNYSALAKEVFELQNKVRQDPSLMIPVLENRLKYFKDKILHLPGTQATNTKEGANSVQECIDFLKKQKPVGPLVYEKGLEEAARDHAEDLGTNGMTGHEGSDHSTTKQRIERHGSWKPGTIGENISFGKSTAEDIVIQLIVDDGVPSRGHRSNFFEPAYKQVGIFGAPHKAFKHVFVFDFASKFSQGGKQEQDGDQEKDKKKKQQEKKVEKQKQKDDDDDDDVEKDGGKEKLIPGAKKVQKKVKTITKGGKKHIITTKIYTMNDGTTKEEVTERVEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LVJ8</accession>
    <name>A0A8S1LVJ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0430019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8068826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LVJ8"/>
    <dbReference type="OMA" id="LYVEMID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8068826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="258" mass="30629" checksum="50BC0F42AA000956" modified="2022-10-12" version="1">MSDNSSEENSTSSREINMQQMFIKLYVEMIDFKDNSKAYIEYFPQFDLGKMIQQNGINIFKLTNKLKELGIQLEGRTISYYSFDCEMYINCGMDPVHFSYIIPFEEIRLSNQLRIKCLQTSISLIHLVMSEEMNEKVNKMKEMDGNDQQNKQQQGQEIQKQCRRTKERRIGYIIEKVSKWREYYSGIMIDGENKRFTLEEAANKVNISKKSLDDYLLQIRYGRKYGFNFNEHKNEKVGVLRAFVKKNNCPKKKKIKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M2S8</accession>
    <name>A0A8S1M2S8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0520132</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M2S8"/>
    <dbReference type="OMA" id="NINAHHG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF85">
        <property type="entry name" value="PALMITOYLTRANSFERASE HIP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="369"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="82"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="324"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8074207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="494" mass="57122" checksum="64984DDF7EE19E48" modified="2022-10-12" version="1">MIDDSQQIIKELIQSNSIQQLIEFMSKVKPNILNLDCGKKQTILHIAVQRNDYAFLKYLEQYSFDNYKENEIRKLVNTYNIEIFTALHIASYNGNVQAVKILINMGADSALKSGSGLLPIHAAAQGDQPYMVWYWMQQGISINAQDDAGNTCLHWATYQNCELTVSYLISFGCNIDSKNNEQQTALHIGASYGQSKVVKKLLIKGAQRNIQDVDEQLPINLAESSQIKKMLENKIDLALFYNLKQPYHPIKRNRKSVSVYYFVILTSQFCIGYILWEMQGIFLQIFMVFLSILLIVSILAQCCDPGIITLHITVEEAMNQQIDPINICPDCWVIRPQRSKHCEFCKKCVVVYDHHCPWINNCVGAKNLIYFYVYLMTLILIQIYSATIIVLYFFQSESLSLYNYLLMLYPILNILIFITPIFLLCIYQTKNLYYGITTYERITGQKTNMKSSLLDPQENIQDSQSEEIKIEPSIQNCFNQCFRNQNKFKKYTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4N0</accession>
    <name>A0A8S1M4N0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0510177</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4N0"/>
    <dbReference type="OMA" id="GNFAETH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8073662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="296" mass="33914" checksum="71E31979FB2E7416" modified="2022-10-12" version="1">MKQNLEEIAATIPNFKMVDGKYYIKTTKLGKGNFAETHLATLKDNEKIVYACKLIAKQNIIEKLKKSNNPDSRKEYIINSLKKEVSLWKQMDHPNIVKFIDFSETPNNIYFFLEYCNGGDLDMLIKDKGKLNEQEAVDIFLQIAEGCSYLYDKSVFHRDLKPENILIHNGTAKLADFGFAKVIEEDKKDVAAHGTSVGTPYYMAPQILAGEDYCIKCDVWSLGVILYQMLFGVLPWKDTNSIIQLLNAINNQKIQFPNTNPVGQEMKDLITKMLQKKEEDRINIKEVCESLNKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4R1</accession>
    <name>A0A8S1M4R1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0470255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4R1"/>
    <dbReference type="OMA" id="YTASCKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="362" mass="43244" checksum="F8CF1C50F9AE833C" modified="2022-10-12" version="1">MSVFANSFRKSSQQMQENDSIKVYYKTDMKQYIKVKYNRGTTVGMIIDELIKELNMNGKLQYSIYLLTHKKNELTLLSVSINRKLRRNEQPSRILFLTKAYKFSFYLDYLLSQFSQNICYLNQNQNENKQLTKYDKEFKIQKFTKNGSSKQMKIKINSQGAVLCQEGKMEKQIQYIDCKFDFEEDLVFTITKDKINIYRASNKKEFSAINKLISQYTKNFEMQYKMGLQDNTIQYYTKQMMNCIEFNCYEFTQSKQMMKENSNYQSFIDIYKITQVNKDIQAIENDDLNINIELYKESILDTFTILPREQFRKRLIIFNSQFKYTASCKIEDQIEQHNTLLQPLDSILTSEEDIRKFSQLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5D7</accession>
    <name>A0A8S1M5D7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0500146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5D7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="402" mass="47742" checksum="3B99241A90C8775B" modified="2022-10-12" version="1">MNSQFQQGRSQSIRFLQTETIYEQPFFTPNFDILSISENLYLEKIKKQATSKDQSPQKRQESPNKPQIQQPKLVQHQEIHKDVKVLQVNYNEKAIQPIEIISKPTIEHKQLIGRAKVLLLQQRSFNMKKYFKIATLAIIAAIRIKKSLNYSLWKKYQMQLKNISFFLKLEIVQTERINAWCKAVCAKSLTSVNQQLHNFIDYPNDKEKVDFSMMKSINSLQFLLENLAFFTQPKYFCEELKYYLLSQVFLDHKAYFSRFVSNRTQYLHTKNRQIKQEELTMILVESILVDQILVNIFDSNQKCLFLLKLVASLLQYYFIKKFNKIKQKKTIIQKFSAHQIIQLENKIKLNLILNPLNNELLIIGLYRDEEIQNLLNYKNTTSQQMDQNFERFCCNLYTTILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M981</accession>
    <name>A0A8S1M981_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0560052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M981"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8076329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="10622" checksum="F0438839372D0ADC" modified="2022-10-12" version="1">MEDLFMLINKYQSHLNQPEGAYYQIIEKDNHQDNRKLNPIKIMIVVFLQIYLYSLFTNTFQMIYTLNKKNLNLNQISNKHLMLTLINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9X3</accession>
    <name>A0A8S1M9X3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0530058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9X3"/>
    <dbReference type="OMA" id="TAECMED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="230" mass="26613" checksum="17882193D9DB0011" modified="2022-10-12" version="1">MKTIQLIPSTEQVVTGQTWIGGPTYELQKQHLPGYAGHVRGLKAESQYGKPFAKITAECMEDRLARCTNSEERERLQTSYKIEFGEPNLRKPQLQTAAERILEDANRQEKAINERNYRTYQSMPKTINDIPPIDRLPVVGYQGFRPVFRHPLKQVVPPEKPQPFVHPLNQIDKEMAKTMLETNDKFRQTYETQKPPIVGYTGFMTGIKAENMYGESYKDISNQVLQRKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MCA7</accession>
    <name>A0A8S1MCA7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0590050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MCA7"/>
    <dbReference type="OMA" id="ICQSKSK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="79" mass="9463" checksum="626B7FCD9781B363" modified="2022-10-12" version="1">MGICHSKSKLKRSGAFHCIPIDFDKLKKYNQERQHNDFNTVKQQSFCQLICQDANKFSNPQTQTYNYDQTQQIEFKKHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MGX1</accession>
    <name>A0A8S1MGX1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome alpha-type subunits domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0620247</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MGX1"/>
    <dbReference type="OMA" id="WKACANG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03750">
        <property type="entry name" value="proteasome_alpha_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="3">
        <location>
            <begin position="2"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8079880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="231" mass="25482" checksum="6D23EEB22ACEABFE" modified="2022-10-12" version="1">MTDFALTTFSSKGKLLQIEYALNAVAKGDTAIGIKAKNGVVLVVEKKQSSILVEESSVQKVSLLTDNIAATYAGLGPDFRVLAQQARKLVKKYDLKYQEEIFVQTLSRELAEDVQVATQRGGIRPFGVSILIAGYDEEGPHLVQLDPSGAYYSWKATAIGKQAKNAKAFLEKRYNADMEIEDAINTALLTLKEGFEGQITPTNIEVGVIRDDHVFRILPPSQVKDYLEELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJJ9</accession>
    <name>A0A8S1MJJ9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0620141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJJ9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="207"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="459" mass="53484" checksum="85E54387E568EEC6" modified="2022-10-12" version="1">MIQQQGQQEEVQKQETKDEGRNASSYGEEGRKGQSKRRDSSNESYSNQSSSFENKIGKRYYPHSEFKIHFYVRHVHMNGMSTNEFLSFIKRKVNLAGSCEVVQMFNTQGSYCDVAIGMSNDDNAKLVYIGKKHFNFGRNGRQKEVVELSDAFKNYLENKNMLEKEHLERSLRRNQQSKGRSDSSESSRRYKSRSVSKKKKSKKVDSKQSQGQGSRRRSPSSSSEPRRIKQIKKNRQSSRSSSLDLNKDQLNVQPIKPQQVYTDKNSVYIFSIPQEVNENDVIQEIVTHHKQNAPIHQIWNTSDKMQYLELQFQDENTAQYLVNLRPCLQVKGIPLLVVAKKQRPVQDLLRNYEVQVELDKDVPPFNVYLEFKKYGDLIGIYVFQQNRNYLLLYSSSSQLQEALRPPVHLQLVINDQNVNANAKIIDKPIDIQRPFIKETLAYQLSTQQDNKDRKRKDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJQ7</accession>
    <name>A0A8S1MJQ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0610154</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJQ7"/>
    <dbReference type="OMA" id="QRINTKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="336"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="398"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="451"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="531"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="684"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="778"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="835"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="46"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="454"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="531"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="572"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="685"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="699"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="778"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="798"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="842"/>
            <end position="876"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8079162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="883" mass="105082" checksum="F00983C3D3F34AF0" modified="2022-10-12" version="1">MSQFSPRLTKVSTKTGFIGTFEAELFAQQQNTQLRAQRMQQIRKQETDLTKKKIQQQQQIQQKQQEEEMKKQKYQEYLQKKAQIEQLEQARQQTILEQQRQAREAEEYRQREYELQQEKLKMQAYYEEQAQKRYQEAQEWKKQSFLETSQGKALQIQNQRKEASIIEKQKEAQSVAIYKETSKNKTQSTFTQDLESYEKFDKNISRRQILKPEDFANTCFHNPQVVKHDIDLEKENAMEKANQTMIETQAMLLEQARQKREQERAQKQRSQKALSKERQIKVLEQLEQETKFIKKQEIIQLQKTKPPTRKPKSEKNHQKLLSTEFDKMFNCNNSSIEVRDHRVRSNSPKKSLTTKNKEKQRPKSVKIFTKAPLDTNLLNDPQKESLVKEDYTKYAIGSEDQSTSQKKKVQFSDMKNESPQSFQNKEIEQTLKISNSKSPYQHHFNNELVHSNENSEEEEEQNNQEEQINETDQEDQNEEQQSDVDSPPQLKSNQIQDIRLYIKEQEQYLQKLEQQRINTKMKIKEEESNLVQSFKNTRKQQFSKQPIPEESEDESSYQQSPSQMNQEESDRQQQSFQQQSQFQNQDQTSPETQNLQKSSYFETIDNFDTQDIMEQFLKPKKTTNNQNKQLSLFADPQSSQKQTISSQQKQSQFVTLQQMNSPNSELNYSLSEVKNVTSPIKKYEFQQTSPPLPQQNESSLKKDSIDSEQMKYSLQQSSEQLQDLVQQEYEETLGEIFQKRKAMLAKKFENRQDSKTIEKGGKSKEELIQIRKEMLKSKRQVEKEKQIQEESQQEPSEQELRPIEQQEDKRASLMKRLAMGEKVKVDKKEMKELTNKNYELLPEVKKKKDEQEKNENIKSRKQKVKEMDQKLRETMKQKRQQCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJV3</accession>
    <name>A0A8S1MJV3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0650007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJV3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="48599" checksum="F63238D85ECEEA98" modified="2022-10-12" version="1">MIYLQKVPKAFILKLLQQDSVKIIPSTYLKKTKIVKFSFQFSDFLVTYQSRIILIQKQEVQIMSLNFTNKFTINQQYINQHFKNIEFIPTQIVINTQILSSFLLINNVKPVIIVSIDKNNLPIPISLFSADISIKYINLVNQQLILSYFCNNNQKICFQVWMLTIYPNSILQTDYIVQIIIIIYWYHLITCFSILLLAITHYHHQFNVIGHFMTTITDKTTLINLQYFTIIVFIYFQKIQLFKFFSKNDDQIFSNSTHYLQIIYNYTITFIINQNVKFRFQKKNQIYITPNNSVTLYLNFKMLQPQIFKTVLTKENLNFQENNYTYPKSLILNRQIDYCGSTIDNETYMLNELCSLSYSAPNSSNIPNSQITIQSFQQKINFLPFRIINPLQFSMTTQHKLQIISIRIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKM9</accession>
    <name>A0A8S1MKM9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0640246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKM9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="194" mass="23020" checksum="A508348D4D6FA807" modified="2022-10-12" version="1">MIQILIQIYRIQQISNNCQRLYQNSFFRSSKYFQTNKESKNINQIVLLLVMDVFELSNAILRPVKILVYQDHNAFQLIRIQKRHLALQRVKQFNLIVIIQSSYIKLIRLLIKIIVLWTELSKVCRDQDCEDLPVIINIHKNKMIFIRFINSTGPGFITMIVCFGSKNQSLILRMEDQEDEDGKIINVEKQFFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ML39</accession>
    <name>A0A8S1ML39_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0650069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="171" mass="19468" checksum="CE78E553174D3566" modified="2022-10-12" version="1">MGVCQTKVKQNPAGLKHKTNRQGERVKTEFSATQETGSKHPGLLQINSLEEINSPKSGGYLLPRRQQTKAGLMKLTGLISNSPLPGQIQIIEMSASKKYSLIKKRQMNDMKQVLSNNKTGIIVEMETFLKEEPETLEYINWILKAQLEXYHLCFKRSLLILFCYKQLCCII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLS2</accession>
    <name>A0A8S1MLS2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0640038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLS2"/>
    <dbReference type="OMA" id="NKQAPKR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8080599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="160" mass="18383" checksum="100034B19F8BDC08" modified="2022-10-12" version="1">MISEDLEIDLLQQSNIESDNDQPENKKPPLTYHKIRVSTKIKRGRPPQPKIKAPISKTQNTEERKSDECPDIIMIGSSKAKMAKANTIMKKIKKKFTSKQHKKQAPERIKKVQKPLKATTDKTQAKFQSVVLSFKTFLKGLRENMQRALDEGDKILKEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPB8</accession>
    <name>A0A8S1MPB8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA ligase 1</fullName>
            <ecNumber evidence="2">6.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">DNA ligase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0670074</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPB8"/>
    <dbReference type="OMA" id="WIKYKRD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07900">
        <property type="entry name" value="Adenylation_DNA_ligase_I_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000062">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000016">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF4">
        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="12">
        <location>
            <begin position="411"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8082080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="680" mass="78433" checksum="EF07F90B87EED241" modified="2022-10-12" version="1">MQKKIASYFKSSKKGDDSNDQIKEQQFIEENLKKEKLILEQTVCSSKSKQHNIEVKGAVLFIDMAELYEQVGEIKGQNSKDAIKELVAKMFIRIMKENKEEFVPAYLFSILKLGPDYESWELGIGQGIIVKSISAVSGKSEKQVRELMNTLGDLGLAIEKSKSTQTQLNKFFVSQTVKEDVKLTMIQVFNTLQQLQKQEGTGSSLEKERILTNLLRMAKPIEAKYIVRFIEKNLKIGAAEKTMQAALARAFQLYHKGIVEGDYESIINQALCECPNYKKIIDTLFSIQSLKEVPELCHIIPGVPCKPMLAKPMKSIQMIFQRFENMKFTCEYKYDGLRGQIHFENGKVSIFSRNLENMTQTYPDIVTYIQTNYKHLDGFIIDSEIVAYDVANNRILPFQTLTSRAKKNVDQNQIEIQVCLYIFDLLYLNKQSYLKETLEKRRQTLRETFKEEEGKLKFATSRDSENFEEIEEFLLNSIKMGCEGLMIKTLEINSQYEPAKRSFNWLKLKKDYLDNGLSDTFDLVPIGACYGTGKRKGFYGSYLLACYNEDMDQYETTCKIGTGFSDENLEKFFQFFQEYLIPHPLQEYKCDGVNMDVWFSPVVVWEIKSADIQISPIYSACSTILQLNNKGVGLRFPRLIKVREDKKPNEATSSQFLYDVYKQQAVVASNNTFEQDDDFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQF2</accession>
    <name>A0A8S1MQF2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0680114</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQF2"/>
    <dbReference type="OMA" id="HYVIYEL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="508"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="694"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="763"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="721"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8082698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="911" mass="108262" checksum="398F13934643F000" modified="2022-10-12" version="1">MILIIRMDQQESARSINISSSRYSDKFQPQLIQMNQKQIKLNKEDSQEFSPIYEKSQDDIIQEKQQRKTRGNFKKSLNYNQTSINPQDKVQNIRGNKIMQLIKQKNLLLKFKERLFQLGHIYPKNPNEKLSHFLEEQYIHQNHHHSKFSQQEQKFEQGIIEQYQSVENVQQGIKIPIINPTGKFYLFWQLLRLIQIMFLLWWVPFKISFNPPKTITMSYIENMLIYLFAVDLLIKLNKGMIDQGQIIYDRLRILKHYVIYELYEDAIYLITLTLVIGGDPIAISFFPEMIVLIQFTFNFIKLKKTINRYGEMFAIQSTFTELVSLSQLIILIFYFAHFMACIWYYVGNISQESFSNSWIQQQHLEFSPLFHKYGYSYYWATATMVTVGYGDVTPQNIYEVICAIIMIFFASVVFAFSINAIGVIFSNIDLQKQYYKRNLVLINQYMNSNEVSLQLQSRVRNYLKYYYEQQVKGNNTEINSIIEKLSYNLKQELLEDVQIRAVTCVDFFTKNFQSSTIQEIACRMNIQQFTPREIIYQQNSVDEHSVYIIQKGEIQLIDDKSGKIVSKLIKGQCFGEIEFLTTQKRQYKAISTTFSSIYRITREMFLDIISENSIDFEKYCEMRDKVIFQYEDLSVKCFGCDGDHLLSNCPYLTYKPEKEFLIKRQLYHSRQQRSQFNRKKLRYLRIFKQNEEHELPQRSSNKQIEHESSLVSQESLHKQDSNLTGSEHQNYTNNNNNEDVAQQIKKRISLWITNPDQSFIEQQKSLQKTNRHNSETPTHKPTRKSLFSSSQDHYSKRHMSNQLLQVNEQQQLMSSSNNSQTKTNKQQNFNQLENLQSYHTFNYDLDKIETFQIYFPQNNIQNVINTFQKQQKLQKFTKIQLASNKYRFIYVQKVPIQSLTKKTILKVNSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0T8</accession>
    <name>A0A8S1N0T8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0730163</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8085119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0T8"/>
    <dbReference type="OMA" id="MIFQQQA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="330"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="330"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8085119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="355" mass="42206" checksum="CD6C493FFF419D03" modified="2022-10-12" version="1">MLTNNQYTQLPVQSVLYQQQPQTNQRLPIQQFQSPVQMKLISPQPTMVYQQQAAQSQLYTQSNQHVKTIQQHHTHHTHEYKIPQYQQPIVSAPQQPIIAAPSPPQQPEINEMYHVERELQVLSVEDVEEPWKKKCVELEIKIFDLQTELARFKNQGQELKVTSNEEFQVRELEAKIKMMKDIEDALRKEINNQQSEIDSWRQRYSKLQLEFQQLLQGGEEVRQLRDALAEKERQILKLENALNSQNVEMQNYMRLVQNKDQEIAEYKQMIRQLEQEMSEYQQTTEKLKYYSNEADIWKERFMKANQDFHTAQEQWMMAQAELDSLKKQKTMTTTEKTTTIQQTNTRSSKAGARQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4R7</accession>
    <name>A0A8S1N4R7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cilia- and flagella-associated protein 69 ARM repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0790125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4R7"/>
    <dbReference type="OMA" id="TINSDLC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048732">
        <property type="entry name" value="CFA69"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048733">
        <property type="entry name" value="CFA69_ARM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14716">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14716:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21049">
        <property type="entry name" value="CFA69_ARM_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cilia- and flagella-associated protein 69 ARM repeats" evidence="2">
        <location>
            <begin position="25"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="676"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="680"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8087768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="844" mass="97824" checksum="B88165B24A1D6FA6" modified="2022-10-12" version="1">MSTHMKLGLSRSLKDTSPPQKESLSIQQVLEMFHSPYTEQLTDRKSEAIVRFCRQRSGGFLLEEIQELIEILRLSIAENNALMQPAIQKICETASVPFIKLRTSDQLKFVPKLSEFLDTLKPVLYSPQQESGSQQPELRMPVIKFLKCFAEEGISEIMKEEASFEQQKKQTELSPLQHLLKNGTRNLRALNQSNLIENIIFTMQHYSKQYEYLLPLLELISSCILYRDLAAKFSNFGILKDIVYVIFECEDFRSYIVKSCFEIIWNTIEAVGVASIKLFAVEDIISGLKKLFENIMKQGYKLEDKCLRNEILILLNYLMRDEKALQYFVDKPQAHTIQSQTNFLEVLLFYATIDEVTFYNEPIRTNQLKAFFGTTSEDLEFKKLIWSGILTAVQSGNQAVLEAVKESPFIITLLLYIDPLANSYAVNRWSPPQKKEIQLHCLGILGNAIVYLKDDFYEKNGLFCLTKFLTNTQELDHKERCLKAFNNASEFEQNYKIKICDEGVMDNLIEFLQNENDNDNPLKIKELCFSIISNLCKECNKNKKLFRQKGAVELMVNALKNPNLGTSARYALYAVSVLDCLWNTILGNRKSEAIFLDSEGLYVLLEFLETCDDMHKKLTLSCLSYLMENPKAIPYFCDWNSPKTMINSTQLLVKIYVGEDQRYGVKYSDGIVTNKLRPLNPQNDPSLRISNQQGDQKSKHMKSLRTQQEDLESDVGSEAYLVRRITEKSAEYDLRAIVFAILYRTGFDKNELLPNEKQMVEVIQLYPHFKIGEIWREIKYDLEQDKIKPTSDDYHWLMTSIEESEELVMNCMNTQGLIAREFRKVQEEELTKFYDIIRSNKLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N559</accession>
    <name>A0A8S1N559_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0730033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8084876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N559"/>
    <dbReference type="OMA" id="EESYPCT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="162"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8084876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="480" mass="56072" checksum="AD7912C1C370989F" modified="2022-10-12" version="1">MENQRITKSLKSTSSLNSKQSTKQIQQPNVIIQQHPTQLIQKSVQQNIPLIPLQQVNRVLPTRDTPIFKQNQCELYSCTKDATNLMAKLDSLREIKSTDSPITSHVSPIPSTQQSVIVLKQNNPSKSQQLLQVACSSCVEPYKVCNKNVTQSQQLSKTLPQKKNYEEMIEILMKEQEFLVQQYNDQVNIFENKMKELSQENNNLVEQNKLFLEQNLQLNEEVTQLKSKQISLQNQSINQNTQKTVQLLEYQLEQKKEELNQLIEKMNEILSINQQTQEKNNELSQQINTILEQKEKEINAYFTQITMYKQTIQDLELKITQLLQNDQLQQKTLKEQQNKLVVIEQSERNKQQLKNNVKETFEYKQLILELDSKCVTITEKDNQIETLKIKNKMIQQQLAQTQEYQISLKNLQEVLIQRDKENEELKSDLINKDQEIERLNQSLKMVRKEKAKLSINLMNAGMANLVMLSQSQTQQDEIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6Q5</accession>
    <name>A0A8S1N6Q5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0810111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6Q5"/>
    <dbReference type="OMA" id="QVKTHIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="355" mass="42106" checksum="AC5E7F3331B57C6C" modified="2022-10-12" version="1">MKCQYHEGQVKTHICLKGLCKHAVLCPICIAKDHQNHMTIELLQFKRSVANLILQHDNPEPLDNISELTLQIAQFDQSLQESLKLLYQKRKETIEFEHFKQVEGILKVEHPTNSQQMALKKVGQHMLFYKDQSNQWTFQLVADNQEYKIWDKLIQDLRNQVKQQTNQLSKILDSLQQISKLIDYEVKQKKVIINTNNLEVQNDGFNISKKIKAEGRELEIQQQQEQDEFFKAEQQQSIRLLRMLENNSDSEDSEPKIIHQIQQQKPNLQTQTTQQLESQQPLLFTQPDRKYIPVTEQYKDSDISKQGQVREKRKYVKKADKLAMMGQKRIYRRKTYDRFKFNTNQCIAVFKEMLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N9D7</accession>
    <name>A0A8S1N9D7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0820038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N9D7"/>
    <dbReference type="OMA" id="NYDPKKC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000810">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF68">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATG1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="293" mass="34518" checksum="366D6DCCF1E4E00F" modified="2022-10-12" version="1">MQQNPSQFDLQKFQQNYQFNNKEIIGKGFDSIVYKVIKKQDGSLYALKLTSFKDLQHKFNFQQEIRLLYHLDHDHIVKFYGNSLDGSCILIEYMPYGHLAKLIDIGLDLRIIKSIVNQVMNAIQYLHSRNVVHGDIKPENALISKDFVIKICDFGFAIIANQPNAKLRGGSEGYTAPELLINENYDPKKCDMFSLGVLFFVLYMGYRPFLSTHPQKQDKFWNFIKNKEWDKFWKIFDPKKQDVGFKNYIQKLLCADPNERYTIEEALQDNWFKSNQYTVKELAQQLRMLLIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NDP8</accession>
    <name>A0A8S1NDP8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0800056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NDP8"/>
    <dbReference type="OMA" id="YNIQLYG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="166"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="318" mass="37490" checksum="0AAC550D97F45C92" modified="2022-10-12" version="1">MNQANLIQCQVLDPQLKPLLNENNTIPFNRKTLLIKFNSAKLCQVVIKLNQKPRKIVATLSNLQNQIHKSNQQALEFHYFTCENQQVIYNSSINTNQQFTSLQIKIEQDDVAFYNIQLYGEEAIKTKQSTLDNFILKPSQLDNENGTFQNFTNALSSNRDPAGRQKKQVNKVALKQKQELEEAIEKSLRSAEYEKMNQKYIQESKNNVIQEQYNQEYQFQEDEELSLEILMQKNDQQQYSYDKKKQPQVRIENLGYTDKINTAQKKGYQQQWNNSDPIKLNFMTKFKVGDINQDFMKMHQLMEETKGVKRFLENVMYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NER0</accession>
    <name>A0A8S1NER0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0810064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NER0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035837296" evidence="2">
        <location>
            <begin position="16"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="155"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="294" mass="34115" checksum="45F6378A42211CEC" modified="2022-10-12" version="1">MIVIIILSWIQLLTTEQLRTQRRKSCIKIKQFQVYNQNQIAANTQQRDETIKALAVSEEDIRVTIQDIANNEATYVGEEATRNQQHETFVFKVAAIDAIDDAAKLIQHLSLGASFAQIKTKFDTLHKKLSDNTSHFALSQPVIAALTELATHGVNQKALKKIAQLLSEIRQQLVQEKAAKTDIEERQAAHWAEQDWLKERPNWKYKFKNKKIPLKMLFHGLNFIHQNWKIVMKDLLDNKHGFLFNHKYMKLKQLKELLNKKLLTDSKNTLVKNCLPLLNLLQAEIDFISLYQYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NFD1</accession>
    <name>A0A8S1NFD1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0850042</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8089989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NFD1"/>
    <dbReference type="OMA" id="PINTYQS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08215">
        <property type="entry name" value="STKc_Nek"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="40"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="720"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="792"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="382"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="792"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="819"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="840"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="857"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8089989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="873" mass="102641" checksum="7F044BBDB43C035B" modified="2022-10-12" version="1">MKQRPTTAKAPLTMDSKDIHQLYELDSRQYQLIGSNLSHFIILNELGKGSFGVVYKVKSTNDGLIYVLKKINLTHLKPKQQAEALKEAQLLRTLKHPNIISYYVSFIEQDNLCIIMEYAEGGDLQKLLKDYKEKRKSLKENTIWQMSRELTQAIQHLHENNIIHRDIKTLNVFLTKDKHIKLGDLGVSKIFNSEIALDGTRVGTPLYLAPELVQHQPYDYKVDVWALGCIVFQLATLEPPFQGENLITLGYSIVNHSPKPLPSQYSSQLFQFITKLLEKIPTLRPSIQQINSLFFEKQGGELKIQQHIEERTSPTLNQNQHHYQRPLTGNLNKQITPSIEFDKVNKIKKSILQVEGNIDYLYNVETNQKVQKQNETDKIIKQQQYQQIITEQQEKLEKEKLDKQREEKIENEKQIKLEKQLLSQQKEKLNKLDKLIQQNQIKIDPNHNEYQLQKPIQKLQHNKVNLFIEDYDLKQSCDEQKSAFSVDKHPQIKFKYQKPINTYQSNIQIRPLSAQARCSFVNISSKSKLIPVLHHNQSAQETKKFTVLDLNPNFNEHSYHQEIPLSQPSLIKQPEKNEEQHMNNVNNKEISLNKTKENIRIKSVFNLQKTNKRFKNQFQEQSNQIIKHYTLKDLEEYQRGNQKQLLFFNRILSMVIEIQNNNVQIIQVQSKQSIQELTLEPKSKIEIKFTSKLRLITHHISTFKIPHYQLLQHYQHIEKLCLHVSNLSKRVTDAELKEIFEKYGQIEYATVCMEPRSKGPGKVSRGFGFVKFAKKESVQIALEAMQDKELHGSKMKVEVSKRAEPRRKTPGRYAGYAYRRSRSRSHRRRRSVSSDSYSSSRRRHHSRRRHYKRRSISKSYSRSPSQSHRRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJF9</accession>
    <name>A0A8S1NJF9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0880093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJF9"/>
    <dbReference type="OMA" id="QKQINPR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="114"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="269"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8091489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="677" mass="78827" checksum="5B8D24C0124C89E9" modified="2022-10-12" version="1">MTSFLQSTFTISAKPNFEFLQNQVDSDSDNEEQKQKLQQVHNHQKEHKKFKKLIDIDLVKEELRSRPNIEKQLNSIQKKSISKIKSVRSLNDLIIPTSPILIDQLGNLNKIKEIRSEVPPELMGRIYSEFRYEFVPALNPVYRQGDQNKRFYIILEGKVVVMKPKLKMVGSSKIEIDNNLEQKNQKKNEDDPFGLKILFPDYIILKILFQGDSFGEAAIKLDTTRSSTVFTLEDTHLLYLNESAYLEYLNPYLSVALDKKMKYFGQTPLLKNINPEDYMGIVLENKLITMKAGEIIYNEGEKTKYIYFIINGEIELLKQVRKKSIILSSYGEFQCFGEVEIMMKINRYTQAKVISPRLNCYRIRKRRFFDNLGNYGTYENMKKHSSIIYKHWQLICNNVQKSINLRDEVYEAAQNDNSNKPNLLANSILNKKVMESQGQKLSQIKIFQNLADSNFKDLKQFSNDSQYLLQRVYSNTLQQYQAKLLKKPQLAFCDTDSSCIRNQIQKKQPLNLSINHDNNSTTNPLNENSIDLKLSSNRSSVITKRDSQQMVSLPSLHPSLHLIKPQPQSINSVLESISKLPRVPKDNFMLSLMYQQAYKADNPSKKAKEIQQIIQAQYRNVQNKFQPKKHNLTEIQPVSNKHRMKKNFKGFLSMRSEQKQFLNFVHSKEIVEGNQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NV61</accession>
    <name>A0A8S1NV61_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SMP-30/Gluconolactonase/LRE-like region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0930047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NV61"/>
    <dbReference type="OMA" id="YECLAYP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000174">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051262">
        <property type="entry name" value="SMP-30/CGR1_Lactonase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47572:SF4">
        <property type="entry name" value="LACTONASE DRP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47572">
        <property type="entry name" value="LIPOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="2">
        <location>
            <begin position="40"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8093385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="297" mass="33380" checksum="F98F47D690B40DC5" modified="2022-10-12" version="1">MSAVPYEKICELASPLYFPLEIDGAIYMVSQNGDILKFKDGQFKAEFHISGQPYSIVIDKLNKFIYVADMAHQAILKRYIDENSQEQITEFLKDYEGQPLLGPNSLLLSTKNNLLFFTDSGPFGETSIENCKGSLFAIDMDAQIVKPLALQCLSYPSGICLSNNQKLLFLCETGKNRVLRFVQTDAGIFYYSTYIQFNGRFGPIACAVSQSEHLYVARFEFGYVSEEGQISIFNSNGVNVENVSIPQCPEISGLTFSSQKSNILYVTENSSTPSCLRILINIEEKDDVKKKDKDKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NVE7</accession>
    <name>A0A8S1NVE7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0980108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NVE7"/>
    <dbReference type="OMA" id="EPRSSYC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8095309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="188" mass="22197" checksum="C9F088AB9CE54F51" modified="2022-10-12" version="1">MLDQIDESIDMTIKMQSLKPQNRSPEQKVSLLKLRKNSKSFVDCTTEKLITIYWYDQEHDQTYEFTQGFSEILRLGDISNWAILKLRDMNLPKIDIEQTKLYLPKKKQGKPNEDFPSFRNELQLKDCDQTKFSLKVTFLIRQVTPPQKIEPRSSYSNSLNKVKHIPIPQQQKKSKNRTNFFCFCNNGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NX42</accession>
    <name>A0A8S1NX42_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1000116</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NX42"/>
    <dbReference type="OMA" id="ECLEDMM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="50"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="379"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8096130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="432" mass="51893" checksum="C559235B5B64BCC4" modified="2022-10-12" version="1">MNERESRMSLGAFSSNPTSAPQNAYATALKALQEKLKGVEGLKKSIENPSDNLIDQLKKQINDLQNENERLQYLLMKTNQESQMNFISSQQYEVPQIERLQHFKDYQDRLSDMIRKYEELKHEKQEIQIVNENLQRQLSVYQSNEKGQIHKNKHKKIENNTNIQDLKTKLDIKDQQLKIQEKKLEFLEIEKEQILQEYTNYKTKYPSIKQHDFEKQIHQLKMLLEEKDKQYTRNIDDIERNKNVREEQMSRRIQDLIGKCNEYQQIIEQLTQDYKDLSLKYQQVKIKLDYQERQHRYINRKYSQGELKQDYKEHKNINRIDISPPLTSSRLEQSQLKQAIRECLEDMMSTQNYNCCSPDRRSENRKQQQILSPMSTSRQYEIETQLKELNEKYENLMNQAQKENDLNNKAVIRKQLIEIAGQIKETTKFEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXU0</accession>
    <name>A0A8S1NXU0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0890214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8092084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NXU0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="101" mass="12064" checksum="B90475C71B1277E6" modified="2022-10-12" version="1">MERVKGWKKEIALFVQILIPDINLEILISVKKDIIMQDCLFLKNVIIKRKGYKKQSESWNFYLNNSVRQFFSGFNKCKCIQKQYDDGQSQVCFECHYSCLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYI6</accession>
    <name>A0A8S1NYI6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0900205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYI6"/>
    <dbReference type="OMA" id="KVYILNT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="171" mass="20097" checksum="51551FC6E647CE88" modified="2022-10-12" version="1">MFEQKFKDLRAKMKPKKVKKISLSLKQQLIESVMNDHLSIHQASKLHSINYSSAKYIINLYKKLGTVAPQQSKHRRKPKFYILNTSVLVDYNSGEILLYKQNQIKKQRQQDNQQITIQKGLIMTSKTIFKTLQINQKNISQSNSDQKQYPTFTDLLELKNFLQIQHKLMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZ23</accession>
    <name>A0A8S1NZ23_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phenylalanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T1020113</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZ23"/>
    <dbReference type="OMA" id="PISHYPQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000083">
        <property type="entry name" value="Phenylalanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.380:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF41">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="139"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="14">
        <location>
            <begin position="296"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8096809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="388" mass="46198" checksum="7BD55B56BD96ABBF" modified="2022-10-12" version="1">MLRRLIRFQFCSLQDQIRKQQNSYSNITDKMISYVGRDLHKIPSHPLGIIKNRIETFFQNEAITEKQSKYPLKYKIIDNLSPIVSVKQNFDSLLVPPDHPSRKKSDTYYINENTLLRCHTSAHQVELIENNVDAFVVVGDVYRRDEIDATHYPVFHQVEIVRIFQQDQFSSKYLQHQIQESMDDLKETLERLTRHLFGDVQMRWVETTFPFTNPSMELEIYFQDKWLEVLGCGVIHQDLMKNCQRLTEVGWAAGLGLERLALLLFQIPDIRLFWSQDPRFLDQFRAGQINKFQPFSKYPSCYKDVSFYVDHKTWEENDLHSIIRDIGGDLIEKVECVDTFYNKKKDLTSKCYRIHYRSLERTLQNEEIDVLQFKIRDQIKDILKLELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZ40</accession>
    <name>A0A8S1NZ40_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1030082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZ40"/>
    <dbReference type="OMA" id="MEGSIMK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="137" mass="14870" checksum="1A948514148FC24C" modified="2022-10-12" version="1">MGICHCSKSSETEQKQVKVKVGGGEDGTCTWKILKGAQQYNLVTNSGWEMKFELSSNGTISGGNKNDNEENALKKIYAGTLKNGILDCIATFEDGAQIKYDGQMEGSIMKVTCRVIKGSDKFNEGDIADCIGPVKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZA9</accession>
    <name>A0A8S1NZA9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein 26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0980009</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZA9"/>
    <dbReference type="OMA" id="FIIRRDY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120170">
        <property type="term" value="F:intraciliary transport particle B binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035720">
        <property type="term" value="P:intraciliary anterograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035735">
        <property type="term" value="P:intraciliary transport involved in cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001918">
        <property type="entry name" value="Intraflagellar transport protein 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030511">
        <property type="entry name" value="TTC26"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14781">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14781:SF0">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04733">
        <property type="entry name" value="Coatomer_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8095113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="563" mass="64619" checksum="24515FFA8F124B8F" modified="2022-10-12" version="1">MIRPGSSMSKAKQTKVSAPVGGGLGVKVVEQKMPTLEDYVKNRDWVGAVALLENEQNFNDSRAEPKLWLAYAYFHNGDYKKAIQVYDQMMSKPDYNKEIHVYKACCYYALCQYEDSKRECSKGPETPLAVRLQFHIAHKKNDEKNLMTYHHKISENVHDQLCLAAIHYLRGHYEEATDAYKKLLLENREYTAINVYVALCYYKMEYFDVSIEILQSYLNQYPTSVTAVNLKACNQFSLYSGKASEEIMKPLQQAYEGGNVFADNDLLRHNLVVMRGGENALQTLPPLVEIFPEAKLNLIIYYLKNDEIQEAYNLVKDLQPTSPKEYILKAIVYAMKGQSNSESKEALKTAQQLFQLVGSSASECDTIPGRQCMSQCFFLCKQFEDVLVYLKSIKNYFTNDDDFNWNYGISCAGVGDYKEAEESLIAIQAEKYKSDDCYLKWMTRTFIMNGKPKEAWELYLNMDPSSESFQILQLIANDCYKMGHFYYAARAFDILARLDSDNEYEDALKGSVVGVFQMVIASKATLDQLLEVMNLLTTLGENPQVEYIITIIKKWGKQNNWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P010</accession>
    <name>A0A8S1P010_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0990201</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P010"/>
    <dbReference type="OMA" id="IERYEEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03704">
        <property type="entry name" value="eRF3_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04089">
        <property type="entry name" value="eRF3_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000020">
        <property type="entry name" value="Translation elongation factor EF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000204">
        <property type="entry name" value="Translation elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="331"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="254"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="255"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8095886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="761" mass="88285" checksum="9E828401EAA86ADC" modified="2022-10-12" version="1">MNYQYGQQMGQYPFDPNMNMMGFDPQMYQEYAYYYLGGPSTPPPPKGPYPGITHEDYESFDINKQILFQRFLGETAAYYAKHLPKYQKEMEEFLNTNTAYQMNEAEKQLMQNYLDYKKKEKEYESFLKQLEQSTQPVNPELEQQKKLEQQKLEQQKLEQQKLEEQKKQQQLEQQKQQQQQQQQQQQQQQEQPKEGTTTTTARPKKKLNLNAKPLEIALNPPKMPIFPKNPDFLDFDKFWNNYSAYINFYNPSCEDEYKNYPKPEQQKKKEADEEAKRKKKQEEIERAIQKRKDAQERAKDKPAQSLNLVEQVVKLEGEVDLQKYVDPDETRQPVNLVFIGHVDAGKSTLCGRLLLELGEVSEADIKKYEQEAVQNNRDSWWLAYVMDQNEEEKQKGKTVECGKAQFVTKQKRFILADAPGHKNYVPNMIMGACQADLAGLIVSAKTGEFESGFEKGGQTQEHALLAKSLGVDHIIIIVTKMDTIDWNQDRFNMISQNIQEFVLKQCKFDNVYVIPIDALSGANIKNKVEESKCNWYKGPSLIDLIDNVNIPKRNEEGPIRMPILDKFKDMGSLYIYGKLESGKIIEGLDVSVYPKKQPFQIVELYNMKDQKMKYAKAGENIKIKVKNIEEEDLKRGYMMCNLTSNPCLVSQEFQAKIRLLDLPESRRIFSEGYQCIMHLHSAVEEIEISCVEAVIDAETKKSIKQNFLKSFNEGIAKISIKNPVCMEKYETITQLGRFALRDDGKTIGFGEILKVKPVKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1C3</accession>
    <name>A0A8S1P1C3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1010120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1C3"/>
    <dbReference type="OMA" id="FCWARSG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="491"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="558"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="607" mass="68580" checksum="4ED7964F89F47414" modified="2022-10-12" version="1">MFDNRNQQQPSMFGVQQQQQQQVFPPQYQGVDPYVQRSPPKAQGTLGFQQPQQSSYVQQTPQQGSQTVYEHKVITTGQVQSPYIQQNPVAFQQASTIKSKGSYCIDWCIYFIPLLFFIAILAFMGMIIAQKDRDFNQEPINNVLRTFSHNQALTPIADIVAPHIVRSSSMHSHFQHKIETELCPEGFTLATLGEWPGINSGCICDKQIKKEGFCWARSGCDSIDSYESKNFQTWNGGRVCQKLTVGWTKLNGNTCSEGYKKCGNVCVPTNGDASRMCPLSDLTLLSTDPTTDSDPTSLNTQVVKIGNKWYRKSYDGIPIVQFQIIPGNAEQVKNSAPCLNDITSSTFESQQSYPLLKKPATGCDQFGNLQNEFVKIDTLTAKKVHEENGILSTLHTIPGYQSYEVESDSYTLEGIRRISVNPISECSSISSADVNSIIEDSEDIYQQRKWFSIIIFWICVVGIVLFVYLYLIRSRNFQTLNVSQIKQPKILILIAVVVAILCIAFGSLYFYNMNGDDGLRNVDARFKSQLENECFNDKGIKNSIQQLHNFAYSQYSTIGYWITLAFWLSIAFLILMIILIVIQSSSHGNLCINPWQSNWVFGYNEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P2G2</accession>
    <name>A0A8S1P2G2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acid phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1000010</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8095944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P2G2"/>
    <dbReference type="OMA" id="INIGPFV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF211">
        <property type="entry name" value="PROSTATIC ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8095944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="50885" checksum="B3BF7FEFAB587A1D" modified="2022-10-12" version="1">MQQLLFLLISVVLAQDDLITVQVVWRHGARNYYHCNWNCIEDQPRGDEAVLTPTGMRQQYVLGKWLRQRYIIDNQFLSSKFNENEIYIESSDENRTLTSAYCNLLGMYPEGPSIPHFEKENESLLLPPNQDAQIPKDLGDNALPHKIQLIPIHTKQKSLHSAIVIQCPKIETFNRETQLYKEVNVASAQLYDDFNTELNLQGEQKVNDIIELSHWRDTFICNRYNGDPLPPNLKPDTLQQLDNIANLTQSLERFQTQDQVKIQSTPYFQRLIEHFDSALNGSQYFKYFASSAHDTTLGCFLSALNLTSAQCQADIYLKKNITYNKCMTKYVEFAANLIFELYNNINIGPFVKVLYNGEYVPICSDFATTCQYTRFRSIITAQLVDYQKLCIESNDETYRVSQTPKWAYIFFSLTILLLLLVLSWILYLRSQQGYRDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P347</accession>
    <name>A0A8S1P347_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1040033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P347"/>
    <dbReference type="OMA" id="RVEYYRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8097422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="338" mass="40291" checksum="4C0B755262FF1C53" modified="2022-10-12" version="1">MANQYNVQILLQKKYSNCQSYYYSWTLNNFINSARTKEVIEFYQAMAYNENLEYLRRFYFANEIRDKLQLFTEYYKYHNEIPRLFMDKISNIMSNYHDKKRRVEYYRIKRIIEEENRKNPNKPKKAIAGDQPEELKPTSPKIQDQVYSKILKDIVDNSTTIEAINKKLEGIQINTGELILQPSNREEEQLQNFIHYLVEKQKPKIQSHIQLHSPKTFNRIPFGVSQQTIKQIIQKNSKNQQTFHLSPKILKDEPLIIQNGPSTHRIIKDPLSKILSPVHIYTMNSPHTIQTKQKLNIQTHRSQQASVSLSKQFKITESMPQNRKFHQHTRSGYRLFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P3F7</accession>
    <name>A0A8S1P3F7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1040098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P3F7"/>
    <dbReference type="OMA" id="VESERDC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015374">
        <property type="entry name" value="ChAPs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="903" mass="105711" checksum="D2BC9C8B406C6090" modified="2022-10-12" version="1">MKFLSIKNIFEFKDNHIDGVRNNLLKNLDQLQGLGLPDVCIVTKQQPTHLMKSSFETQSSFHFVQGLCPQSQADIFAYLQTIFQNQEKQEHQSLFSKIQSIQKLTYICYDIFSRTFIVCEIQVFPEGQHKSKSFGVQENQQYILPSRQHWQGVYISSILRAIDEEFKLASVGRFFNNINLKNNNKLSEVIQLLLELITTNISLFQDLDKISSIKEFPDIKDVLFYLCWPLEILVQYLEKSNQLSILIKELENIEDSVIFYLIKSILFYKLKNYEKSLSCLVQISSICPQLNLIKYIFGKVLIKLGRYKQAFLMLKDTLINSNENQTIWITLASIFRKKNNYQICLSLLNKAAGLSGNKTRKSKWREIHIQNYRVLAQDNSINNLTDLDKVYAVINPIQVNKIESYSQLSNHPVLEQLLLRQRALDKKDLKNWYLQAQKQIINQAQKIEKSTIDFISQNQLLENKQTLTLFQKEIIRLFIQVGQKQLQKYLQLYFYSPPHSSYSFFDNKIDKQCFEIKLKSKKQNNLQKRQPINTSSITLGDIDSDEESEPEFLKQNLNKTFDFTNTYSQSNLQSPNTKQKKSNTKITQQLFEKQISKTYQVIQENAFESVESERDCVNKCKEFGIELLQKSKTMETLSEQSCRKDLKVQISGLIDHLDQNTPQDKQNEIGDLMKRKKDTLNPILQEFVDQISLTQNILDDLFYNNNKKTKSFQSSLQQSFNCSEKQTPKTKIPQIFKSQLKESVKKKNYLSKKIDIKDIYNLYGLSFQDNDEKLTYFQFTKQESIFLYKCSKIATKLKQFELAQKLLEKINTRVISVQISYSLLHIYKDDHPQLIQLIQKTMVDFIECGISKIESMPLWVEIHLIRLIKLKGSNYVLGLLSQLENQDVFKLMKKIVLQTENLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5T6</accession>
    <name>A0A8S1P5T6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1070008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5T6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="45875" checksum="2D56E59B78725FC7" modified="2022-10-12" version="1">MANQQTFYKSSLHSLKSSIQEYYPGPIIQSRQPQQQPPQILSSKYSQLIQQSIKKQNIQPQNQPKLQQALEQRPLEQSLPKQQYVLLFDQIEQDLEKKFQNAVDRTRNLLQRNLNPQIPIKQLQQIPYYDENQSQQISYYDENQSQQISYYDVNQSQQIPYYDVNQSQQILYYDENQSQQIADYDVNQSSKIPYQDIEQQKWILSNKKHNKFLADLFDYEIQSDSQHDNLVQSNQNSQQQSQISYNNDQEQQLQELALQYADGYIYRGQGYEPATKEGFGILTDENDNKVYKGYWHDNQYHGQGKLINFQAEQIDGAFDYQDLSGIENGWLGYEGEFQEGKFYGIGTLYLTNGEKFEGTFNDGMIDGEGVYSTINGQIIKGIWKEGILTHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P9J2</accession>
    <name>A0A8S1P9J2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1090124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P9J2"/>
    <dbReference type="OMA" id="INPRTKC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8099393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="220" mass="26837" checksum="62BFFBA8C14ED78F" modified="2022-10-12" version="1">MLQFYQILKNKNGRVIVELDEQLMEIIINDYSLHQQIKNETQQTQLDEQKFQRRIVPFFLNQFMHWAKQMDYKKVECYLKVIHNRKTSKQQKWELGDLQKIFGPINSRTRCIQIETQQRWKEFLDQQAEMTVIMNNKIKDQQTKRKYIDAIPQLKQELDKKNPYHRFLSISINSKIDYKISTETCIDEHNQKEFEFFIENSDQEQDRLFTSFIQDDKESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PAN9</accession>
    <name>A0A8S1PAN9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">PPRIM_AZ9-3.1.T1110160</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8100160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PAN9"/>
    <dbReference type="OMA" id="NEAYACK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000199">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001499">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="59"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="361"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="398"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="434"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="471"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="CAD8100160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="499" mass="57747" checksum="B2F1BB85D883C2B1" modified="2022-10-12" version="1">MGSCSGKRSQNNSASKNSSNKPETDNSSPQKLQLNDAQSCFGNLIITSEKLGRITKDYTLLNPPLGSGAYGEVRKAIQKSTNLMKAVKIIHKSQTSKEEQERLMNEVKMLQKLDHPNIIKVFEFYQDERFFYIVTELCTGGELFDKIRHEGSFSEKKAAEIMKQILSAINYCHDEKIVHRDLKPENLLYESEKENSMLKIIDFGTSKEFVPNQKLNQKLGTPYYIAPEVLKKKYDEKCDIWSCGVILYILLCGYPPFDGKTEEKIMDKVSKGVYTFDTIEWEEVSKEAKEYIRKMLQFDPIKRYSAQQALNDPWIKRFTNPAEFDKPLMTKVLTNMRQFTEQQKLQEAVFKFIVNQLATKEEKAELLKIFQCLDTNQDGKLSKEELLVGYSKIMKPIEAVEEVNRIFQQVDKNNSGSIDYTEFVIATIDRQQLLSKQRLQVTFHMFDKDKSGSITIDELKEIFSGIPEEMWKQVVQEFDSNSDGQISLDEFFTMMKKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBX6</accession>
    <name>A0A8S1PBX6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1120053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PBX6"/>
    <dbReference type="OMA" id="YYNSNCE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8100331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="120" mass="14647" checksum="642CA98CD23B9725" modified="2022-10-12" version="1">MFVPHKPWYFEKYIDFFDHDGTLLGFEIEDQIYFFDQHGGWADQQGCYYNSNCEPDGWLLPDGKDEILHYNQNKEFIRSYRIQDLDWLNQQEQHRKRRQSAIKQIQILEQLGWDLTKITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PF11</accession>
    <name>A0A8S1PF11_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1150038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="GHMIPIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="27"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035764622" evidence="1">
        <location>
            <begin position="22"/>
            <end position="2797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2797" mass="293506" checksum="99B495FC68E2329D" modified="2022-10-12" version="1">MQIKYLLLIAILLTITKQVTVTKTDECDECTQFKSSSDCKSSKLGCTWTEKTATTPGSCGKTTAPPPVTYTPYCSTIETANCAKTFGCALIEGKCTHFTGCSAYVKTTHSDCQNISNLCITNGTTCTNALECIGYTKEQCETTPSLKSPYKCKLEGETCREYNCTEADSSLATHGACDTWKAGCKTTGAGCIDSIPSCNNYQGTSETCPKMRGSDGNCEFNPEGNNCRPRVCTGADTSLNTDEDCGKYQDKCLTTGKGCIAIRPSCSNYTGNAITCIGYIGTDGDCAGDATGTKCRARLCSEKTATTDEDCGKWKTGCQTNGRQCVDSLGNCNTYEGTITTCAQIKGLDGNCKGTSTTTAKCVLKDCVTDSNETFKTKDQCLAVQQFCKTTGKGCVATLSNCSSYSFTDADGTSCNSLYGLNGRCKAGTGGKCADRVCTEAPTSYTSQPQCEEYKSGCVPTGAGCVSPTLCQDTVKQVTCEGTDNCGWNSICVSKTSCGLLTQSKSVCESVKINERSCKWESNTCRPAKCDDFTATDDTTCNTLLPGCVTNGTKCVDGSNCAAQFKGTQSTCQGYKAKCTNDTNATETTACKTKTCDDSYLEYISDTDCSNYLAGCVTKGKGCIIDTAQCSSYTGTPEQCDKFKGNKTIRCWNTSATSGPCTNKQCNQATGMADNNTCEEFLTGCLYDGNGSCVDKSAQCTAYTGDATTCPGFTGGNGSKPCYKGASGKCRDKSCYDDTTSDSDGACDTFLPGCVTKGTGCIPKTSLCSAYQGITDKCLTFTGNSAADKCTRLEPCISKSTTCSLKTEVTSNNDCLTYHADCRFKLGDNACIQSLGTCAEYTFTSTDDTERQNYCNTITTKTGSLCSYNPTVTGKCSARACNLWVSTLANITCENYNGTSSCKSNGASYCYAPENSCALYTIPNSITEAAAKLTWCNGMFTNDVTPTKCSYDSATSTTCSDINTCEEIISPTSAADCNKKLNNGNCQFTNNKCITTKTTCNGYSLTGITTGQAAYCSALKSDDGTTVKQCVYNSATTTTCVEATTTTILSIPTNPTYTDPSAITVAACNTITSPTSQADCNVGTGSCKYYKGACYARVVCASYTIPASITAADDKQYFCQNMFDDAADTYCSFDSTLNSNAGGCAAGKTACTEYTGLTAAAASGDTDDVANKLKCSKYRIKTGSACNYIQSETVKTTCHAPADNAASCTVITAGITTDADCDLRTILGCKKNGGGATCIARDACTAAVSGTTNDEKATNCQANPGPDSIYCSYEASGANCVAAKSACTGYVNAQATCAGYSGLSGTGLLALCQAKIDQVGVKCSWNIGATACIAAPKTCAEWSTLPTSGQLEFCQARIKSDGKSCSWTTGAACSDAPATCAGWSTLPTSGQLEFCLTKINQNGQRCSWVTGASACSDAPAACSGWNSVPTSGQLEYCQAKYKQDGKKCSWISGTSCTDAQTLCTSLTNLPSSEELAYCQQRIKQNGLRCSWTTGASTCSDAPVACTGWSTLPQSGQLEFCLTKINQNGFKCAWNTSGTTCIDLPAACTGWNSLPTSGQLEFCLARSNLAGQKCSWVTGAAACSDAPADCSNWNTLPSTGKLEYCTTKFQLDGGKCSWTTGASACRDYSCEDTLSPQSQADCDVIGTGCTYQPQNQICYIKQSACTSYTPTGLTDDDKLNYCNNLVNSSNVGCTFLKKGSVTTCSVLAACTGYNVTSITDAGDKLSACQGAVPTSGKCTYFSGNTCVAVDACTSYTGGTAASIGADCAKQKDTAGFLCFGTGTACVAATCNDVTGATSIDDCKKYANNCVYSTQKCYTQSATCNYSTGGTNAIPLCNSLKDTNGNFCTADADSDATCKQRTCDDTATKMFQTHEECKAYLPTCKSNGNGCILETTTCAQQSGYLNYCDWVLDTNNKSTCAKGTAATTDASCTNLSCDLNVTATNDSECQSFHPDCLNNGLGCIHKNEPCSSYYGTKEQCQQFIGNGKKCFGDSTSGKKACRERKCTDMTTATGNSDCENFLPGCLYNGAGCVPKSDPCSAYKGTQTICSGFKGSNGTKYCWGASDTASGNCADRKCSDKQGTTDAECQTFLPPISTSSSQLCITDGTKCTDIGKQCSFFKGTDETCQKFTATDGPCKASAVSATPVACTPKVCYEAPNTFTTDKQCQDYHPSCKTTGRGCKSNVGCGDYTSLTSCTTNTSCQWAGQCRSEPATCSSLTSLGQTICVNTPLTNGKKCAWFNGATSQTSVCRDFTCADYDGSITTHKDCQDKDQTCTTSGAGCVTLGTCSSYKSQTVCEAANTTEDSLRCFWNSATAACRQRQCADGVFTTDDACKTWLTGCKTSGITCIGPTFGCGAFDGNPKLCQQNSAGNPCYYVDGTCYDYDNCTDITSQTFSVCQKFSKQCVPTNSTCRAITLCDKYEDVFSCTIGINDTKCGWLPEGKCKDYTACSDANGANLSACASWGSTCVSDGTKCVDKGTCSSYLTPSACDNDGTDGKCQWTGTSCRLRECTDNAATTDSECLAYVVKSGLCTTDGAKCVPRSTCGSYQSETACTTGQDGVPCIFDLPVGATTGTKSCRPKECTDIKGVTNDACIGQIPNKACVSNGINCVKQDTCANYKNKLSCKAGGSDGKCAFNPAPTVADPNNGTCQQFKSCETGNNDKDACDSKSKACKWSSTTTGTTTTTSCKPMDCPGVASGTTCNPFQSFDGASSTICVLVNNQCTVGDPSTLTVDQCYKTQTLYSYTWNESTNKCVSCKASTNNNNNTTNPNTTDPGTNTDDNGYILGVTIPLAILGIFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PH47</accession>
    <name>A0A8S1PH47_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">cGMP-dependent protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPRIM_AZ9-3.1.T1180167</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PH47"/>
    <dbReference type="OMA" id="QWEELEM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05572">
        <property type="entry name" value="STKc_cGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000251">
        <property type="entry name" value="cGMP-dependent protein kinase 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000154">
        <property type="entry name" value="cGMP-dependent protein kinase 1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000089">
        <property type="entry name" value="cGMP-dependent protein kinase 5-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035014">
        <property type="entry name" value="STKc_cGK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="17">
        <location>
            <begin position="85"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="17">
        <location>
            <begin position="203"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="17">
        <location>
            <begin position="324"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="457"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="18">
        <location>
            <begin position="716"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8102552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="770" mass="89192" checksum="20877DBFD3C6639F" modified="2022-10-12" version="1">MGVCISKITQPQPEITIDPMPIVQPQKGNYSMNSLPAEPRRGDKKRQAKIEVLEEQIHENVVKQPKEKSPFDFQMILNAFGNHFMFAQLDNNDKAKLIDDMYYVTAKNTDYIVKQGDKATLFFIIERGDCKIIINNEVKRVLKAPEFFGELALMYHAPRSASVQAIGDCGFWVLERKKFRKAVEDIQQKAYETNRKFLDQVKFFDFMTEEQRDSIANVLITLKFKQGELIVNEGDMANSFYIIQKGIVSVTKQGQFLRYMNQGDSFGEQALFGNCVRGATVKANNEDVNLLSLSREDITTILGEKIQLIIYTNMQKWAFEKHPQLRDLTKMQIQKIVSNFKIKTYESKEYLFSKNQMIDKLIIILDGQLEFDGQLYNNGQLFGDKFLQVEESKRKISSDIVTTRKTTLSVLTFKQFFECIGGELETVVKKNKDRAGSSSLLEKRQKSNYSLFTLEDFIGLQLLGEGTFGNAYLVKDIPQQNLHVMKCIPKVNIIANNTERHMKNEKQVLELLNHPSLVCFHRSFKDQNYIYLLTEYVKGKELFHVIRDLGLLNSYETQFYIAQMINILEKLHSYHIIYRDVKPENFIVMENGYLKLIDMGTAKVLKSRASKTYTIVGTPHYMSPEILKGKGYTFSTDLWSLGICFFELMCGEVPFAGNEDDDPYIIQEAILNGRVEYPQFLKDFKAIKLMDQLMNRTPELRLGGSYDALKVHSWFDGFQWEELEMQEIKPPHIPKLQQSNFEEIPFLEMIKRDTSPDMICTVVVDWDQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PI69</accession>
    <name>A0A8S1PI69_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1190021</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PI69"/>
    <dbReference type="OMA" id="QIRIHYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051938">
        <property type="entry name" value="Apopto_cytoskel_mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="21"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8102669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="206" mass="24163" checksum="B9841F8B1592E16B" modified="2022-10-12" version="1">MFYKTLKAFSRVSSKIDYTKDYYKILKLPSTADQSQIRIHYYQLAKKYHPESQTANSDKYSNVQEAFRILSDLEYKQKYDQKRVNQADSNLQADQQEKNDNNKQIGISHGIQQQLFEKNDDIWKLKITVKQGQIEHNYYFSETELKEKSINCTEFEKVTSDLITNLKLNQSSIEEYQNSSFSSTSILKKVAEIGFLILSILIARKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMI5</accession>
    <name>A0A8S1PMI5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1220009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMI5"/>
    <dbReference type="OMA" id="WASSKCC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="139" mass="15165" checksum="40E31034283FE3DD" modified="2022-10-12" version="1">MSFFGKVFLLTALAGYSYLLLTDVTLGKQFDTKYANFQQQALIKQYIPADVFKHVPALLAKQIVGGLLASSVLLFICGGFVVFPVLGLLLQIAIQSNPLFSNDQVTQIECLKLTALIGGLLIWATSNCCTKQTTKTKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMV0</accession>
    <name>A0A8S1PMV0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1240112</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMV0"/>
    <dbReference type="OMA" id="NDADSHF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="14"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="48"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="150"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="184"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="218"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="252"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="286"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8104647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="43730" checksum="B81042760601DBB8" modified="2022-10-12" version="1">MCYDQAISIDPNNVDAYNGKGNSLRKLKQYEQAIICYEQVISIDPKYVDAYNGKGLSLQKLKQYEQAMIFYDKAISINHNYVLAYYNKGNCLQNLKQYEQAIICYDKAIQLDQNHVDAYNGKGNSLHNLKQYGQAIICYDQAISIDPNYVDAFNNKGNSLYNLKQYEQAIICYDHAIIIEPNYVDAQNNKGLSLYNLKQYEKAIICYDKAIKFDHNNVIAYFNKGICLQNLKQYEQAIFYYDKAISLDQNHAIAYFNKGLSLNDLKQYEQAIICFDKAITFDHNHVIAFYNKGNTLYNLKKYEQAIICYDKVISIDPNYFNAYFNKGFIPIIQFKVIVYTIQNNMNNQQSAMIKLFQLIQKMLMPFITKQIPYNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PN90</accession>
    <name>A0A8S1PN90_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1240100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PN90"/>
    <dbReference type="OMA" id="GCCATNN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="550"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="558"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="734" mass="87244" checksum="62040884A3A42DA1" modified="2022-10-12" version="1">MGCCATNNNYSQQLHLIRYQTFTEFDISYPEGLLEQFNKQLNELLIIKDEMNKFITTSDFSEEDYLITCRNFFDRYFTLLKTYHSQKTIEDQMIIEKLGKMREFISNLQNEIKSVDRDFEIWEQEFLVNLNCLEQESGELGEFYITAKNEQNLIKIISVLDKFDDKVLDFHQVWLQDQIQELRERLFRQSEQVIVNYSNYIINYMKTGNDKNLYKNLQEISKLNDLKAIEQLGYRLAQQLLHLRENQKDILFKSEFLDMAYKLQQCFTQNYDKYIIQPIIQQMDEFLIQEEIQLSSLTILLEAFGLVKMEIFDQEFYCFHIHKAETCIKNMKEMQQNFEQTFTKYQIQFLTKQKQNLQHALQVFEPFYQLSMKKISQKIAEQINQYFSRLECITLLLNAKKRQEQKLQVFIQNYGSRCSQDLLELQKNSNKFLDLKGLKLNQSTDQVTQMIQEYQISFQKMQQLYLCNLGALITKEADEISQLTEDKPNEQKKRIDILIQNIDVGILNAKNIMIKTEDTDLIEKQLMMALDKLIYNKDQKKRASQLSDYALKQKQQRKSTTALNSPNSETNNSKQIRQSTPKSIIVTTSNNNQELEPWAKLKRIPLKIISQCGKCDNYASYQVKHDLCQTDYFLDMNGFVRCSKRLISSPKEKSCKLKHITDQIFVCPYCKGKFQLNQFHKFIQAIIKGLDYNNLPFESKDILLFFRRVYEDFSKIYYNYQVHQSKSISESPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PP09</accession>
    <name>A0A8S1PP09_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1250042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PP09"/>
    <dbReference type="OMA" id="ENECPLE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="428" mass="50916" checksum="38081675CCBDBB59" modified="2022-10-12" version="1">MKKNQYQIKVTQDSTPSIENITQQDFLSSIRRQDTPRKSKVRFAKDPLENECPLEQPNPFLFNEQLKLSFRDYLEGQKTALNLVNECNHVIGQTHKMDQFKLFQNVNRFRTQLKQRSSIRMFMLQQNSYTDDLLQRKIKVQERYNKIKQLIQQKMKSKNKNDSLRDVQMFKHLFDTCEKNVNKIKSKNLLSEKADGIREYDQIKYQKNPTGSLRVRVNTCNQQINNQMRVKTLPQQSTLHQTIKKEREKMNAKLTEFLEHDQNQERNLQIKARKNLIRDLKKGNKRFHSFEPNQYNLTDPLSKDVKLYAYMFMKPNTDDPILKPSNQLNVLSLSTRTQKNLNRHYLANFNEQLSHSRDQSLDEDSSFNSMLEINIDNLYKRSADLQSKILNTKSEKLVRRIRQFQKVKQLTTQIIKVNELKLSNIKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRU9</accession>
    <name>A0A8S1PRU9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1270039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRU9"/>
    <dbReference type="OMA" id="DDSQRYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027968">
        <property type="entry name" value="JHY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14726">
        <property type="entry name" value="JHY PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14726:SF1">
        <property type="entry name" value="JHY PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15261">
        <property type="entry name" value="JHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="269" mass="31814" checksum="47E214F4F4C09352" modified="2022-10-12" version="1">MSTFQERFKGKTTVMGRNGLESVLNTCQQPIKKDFEYQNRCPQIPQPLQQQVNVQTFPNQIQYNNQQHHQNIYQHNPYIPEQQIQDHLQYLSQQQYFQPSTNYQVRQTATEPQKKIEQRSQSAKQQQQSVDDSQRYKPYTIKDYEMMKKTANAKLGGLGPNISGEEWVKEKEKQLKRQEFAEQVRQFNSTNIITIKNKESKPPEINARQKAIEFARNIPKPVAKKKDDVAMKSMPNKPVNNMNRDPFDDEIARLEREHIKYLNQLDKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTX8</accession>
    <name>A0A8S1PTX8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Swt1-like HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1310053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTX8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="17628" checksum="2D6F278AAD6A24FD" modified="2022-10-12" version="1">MMKSVLQTHSMRQIVGQLLDNCYEVLRAFLEQAIQHDEVSPENTIQINKDLMGAINFYISNYDFIQEQTHSNSKFLRNLLFEVKHYRNNWAHSKDFTIREVHRIADTILMLFDELSLNITNEVYIIVNEIRMESIQKMSLQLQQSQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTY0</accession>
    <name>A0A8S1PTY0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1290065</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTY0"/>
    <dbReference type="OMA" id="VDCCKPT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="593"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="826"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1000"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="826"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1012"/>
            <end position="1026"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8106038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1177" mass="138118" checksum="799E1F7BDDEE6EA3" modified="2022-10-12" version="1">MKKKESPFFGLDNPVYSQNISSPRFEELLPIYSNDNAIDQPLEEALKDKRNIFERNNFGAMKDGHKEESSEDDLYLSSVSDNRYDQESARSRTNSLIRQQRSLSIQKNQQINQNSLKKVNTGLIIDSTFNLQVQGVKSSSKIDKYQSFVQQFELEEDKIRKNKELIQQKSNQTNELSSKRNSGINKGKIGLNGIWAVKGFIIIRLVSRFIQQLKTKTETIKFRLLTRKIFSVIGDFLILKYNFQKRATRLLHYLENCQDFLSRNIIVIRPDSKFKIIWDILLLLFIVMNIFYIPINISFNISTQGVFEYLFDLLPSWIFVAEIIINFNTAYYDKGLMHEDRKSIVKHYLKDNFFWDLIVVIPFLMSNMNIPFVRYTLLFRLTRLAPLMESIEEMLNLEENIQIVVDLLKLIFFLVLTGHFCGCAWHFVALTEYESFGITDNWLTHYDRQAFDYHWFDRYIISLYWSVITTVTVGYGDIVPVTTFERIFVIIVTLLLCGVFGYCISNIGNIFKQMSDKKTTYKFKLRQIHQHIRKRGLNLNLSLKVKKYFEYFFQLEQEEDSHAEIFISQLTKHLREEVLTDLYCNTLKKSRFLRENFKELTINNLCQFVKEKKVLPEEVLYSRFEQPTKVWFVLSGALEFVADHKNENDYYEATETFLKKVSSGAVLGEREFITQQPYEYKVRALKFTQMAYIDYEDFINVISENDSEYEIFCMKRDRLLFNPQYKGSGNVCEICEWTHNFIQCPFVFLQPNINKISSSFTAVRLNNRIKFPYRHTMKSRTRESLNDVQEGALKIIVGNLTEQEYTDDYLVSLGFQLTQNLEIDSQSGDEVSKNLNDSQSPTTSEKQKAFQKKSAKTMAGGITMDLKDIKESHDLKKSIINFQRIQFGKEKQKVLQLHKKEQQEKGIEVLEQSQILVLPNTTTNNKFYQKQLQNTPRRSSNFEELGSLKQSQKLGNVANPLDQDQNKCFMKQLSKNSEASEMQESLIRKQIEIQERRVQIGGEQRRKKRKTTIQLGQKAQRRSYQNQQTNLSPGLQQQLQKQQLVVTLDQKLKRQSIGESKKQMQTNQITSVGQIEFTGELVENTQGDSLQQKILDIVHSNENFQVDCCKPTIMYFPEFNIEAILKKIDYYYLYVKGVEKKMYKRSKSNRNLFERIKPSKNATIKSFLNNSRSQQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUL2</accession>
    <name>A0A8S1PUL2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1310164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PUL2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="75" mass="8829" checksum="4DDD2F69455E0798" modified="2022-10-12" version="1">MITHKNILIQFLNIYFVQPFDDVITAYQMEKTQIINHYPTLKSLSKVYWDKGLKGHYAGWQQPYLLQNGLLGKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q081</accession>
    <name>A0A8S1Q081_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1380117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q081"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8108904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="43" mass="5182" checksum="F326192FD235B712" modified="2022-10-12" version="1">MQFLTLEVNYKILLVFVISAILEGYPDFQENIYKIQKFTLVLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q0U4</accession>
    <name>A0A8S1Q0U4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Conserved oligomeric Golgi complex subunit 4 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1380091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q0U4"/>
    <dbReference type="OMA" id="HEWLEEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048682">
        <property type="entry name" value="COG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048684">
        <property type="entry name" value="COG4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20662">
        <property type="entry name" value="COG4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 4 C-terminal" evidence="1">
        <location>
            <begin position="502"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8108855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="722" mass="84413" checksum="C5F175DEC7370EE1" modified="2022-10-12" version="1">MNSLKDIYPLIEQEEVLYSMRKQKLILEANKLLNSNESDTLDDSIKNSISKVSNLRQDAIVMDNQVHQIQQNAHVTIKSFEALKNQKKNVELLLNTLNCFLELSRIKNQLPIAIEQQDIASCIKFGQVYYEILPNLLKEFCQSDYQFVENIIMNQLIPFQKQQFELAVKNNELEKINLCSGLARVLHLEQSLDKVKNIYGEELLKDLRLLEQNEQQNLPLSFQDIVNNKYHYPFLNYFVGILELIKPEKFSKNVQQYYGVEGVLELIDYLVSKISHPYLLGVLKQLNSFFEIEKMVKNAKSVQSYDNQLLQSISKKINLDQKQLEKVSYYCDEISAISSEYNGFLQHLKQTAELDKDITNLAIGESITEYLSKYLEFSQIELQQQIVFFVQKSEPLKDLWNFDKAKFTKLLECQNVLLSNGYHEWLEEIFYLIKQQIQRAISTENQIIASALINFIGFQVISDDLHQIALQLFHRYLKKEYFSPQAIGISQEQTVNNSFIILLFNILSNIPMYLKTLKSQIYSELPKGPQSQMIVGAVEEMIEVNTKKFQKLIEEKLKVLAENLKSTVKEVFEEIKQLHFDQKDVVMLEQEREYQKYFSNQIKNAFKGLNQWKTQFTQDNYEQMSLHLCYSIAKQLEQIIMNKKFHQTGAVILEKEVRTSINYLITMTNPNNKQAFARLQLICSTLLLESYKEMEDFLSSSAISELSSNEIKKIRQLRIDLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q406</accession>
    <name>A0A8S1Q406_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1410019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q406"/>
    <dbReference type="OMA" id="EIPYAQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="56895" checksum="927E08833EBADBC2" modified="2022-10-12" version="1">MFLYCRSYIPKYSASQVIKNAIQRRKERKNVMQEHLERLANKLEYKVYPYHITHYDPAHPDYIPAQRQDYKQRLTVDTHNMLVDGVKRDVAMQRQVDEAIKNLDRPYLKGKPGVTKNITGGLRDYFPIEIPYAQTGNLENEELEYENVFRNEKRWIAQTIYPNEKTESQKRHEKELESRPVTRKFHPDKGSKYDVETPYHLRFPYLADRLGHPEFLANPFQRLFRLESDMYHPSYNDQPFVQHPSADPDPTLNFQEGEVIYENTRLQEWLKFVWWTGTFSFAWICWVLPYNIVYKTNLMFDHQIDASFYPYHLQSIYNMDYMRINVLAATAASAYLWYFHHMSMNDIGRNYVTKVQYNQDKELIFVTRMGSFGVIREDVFETHHLEVLPYYTKSGVQNMRQNDLGMYELSCLNKQELMYLYKEDAFWNPKLKTQFLSNHTQLISKQYFGLKRNEEEEYQKTQTIGELPLKDLPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5K8</accession>
    <name>A0A8S1Q5K8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1440131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5K8"/>
    <dbReference type="OMA" id="YEDNNQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="727" mass="84996" checksum="1039CE666FAF6F30" modified="2022-10-12" version="1">MDKQLAFEPKLYLNKIQIPIYQQMNYFDNINQSSLKNQTQKQDSLEIDGFDWDYSNYVNRKIRTKLLISHTNEQDLKYIQNGSILRIEKIQSTYQKPELIKNLEQVKHLQWIGEYGKNNLKVGNWKVFWKGENLVDVGGQYSTNGKKQGKWKETIQNYWTKNQVYFLGEYENDQRQGVWRYIYDGKYIGGGEYNLNGEKIGIWEELSEAFWDFNQIIYIGKYQRGKKIGRWKNSLINGNDLETNQQIWGGEYDEEGTGIKSGKWIEISQMFEKLSQVIDIGEYKNGQKSGIWNYFYRDDYKKQFEQIGGGSYDEQNLGIKNGLWIELSDGFEKQSQVIHKGIYTNGKKFGKWEILYRANGMEQFQKIGGGLYDLQGEGVKNGFWIELSEDFTYYSQVTYCGEYKKDKKVGRWDIWFNNGSNQKIGGGQYLQINGMKIGKWIELSDGFYNESQVILNGDYKNGKKVGIWNIYYRQRNVDFFKQIGCGLYDKGGEGIKNGMWIELSPTFEYYSQVIYYGEYEKGKKVGNWNTCFIEKDLFASFNQKQIGLGSYDKDGNGMKIGIWIEISDGFWDNSQITYKGQYKNGIKVGRWNTYWIWDKQNKQIGGGSYDDSGDGIKIGRWIELRDGFYNLSQVIYIGEYNKGRKVSRWDILYSEYGKEDFEQIGGGTYDEEGMKIGSWIELCDNFGNGSLQSQIIYSGQYQNGQKVGQWFENKINNKGCGMRYDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q604</accession>
    <name>A0A8S1Q604_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T1450065</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CYHEEVF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000082">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000087">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000093">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000117">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000162">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000166">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="363"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="523"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="933"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1063"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1137"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1284"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1315"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1346"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1372"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1410"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1511"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1590"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1630"/>
            <end position="1649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1656"/>
            <end position="1672"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1727"/>
            <end position="1751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1812"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="16">
        <location>
            <begin position="297"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="16">
        <location>
            <begin position="940"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="16">
        <location>
            <begin position="1280"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="16">
        <location>
            <begin position="1593"/>
            <end position="1839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="37"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="162"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1171"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="542"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="162"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8110743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2113" mass="249125" checksum="4F1B15EE733A76B2" modified="2022-10-12" version="1">MQKIKDRKTPEKVRTTKKHFSISKEMANRKVNIQNIHSEDGKNRMYGSRHSVSSIQSKQKMNVSMNKGLSHSSSSLLNDSESDKRSLNQSIMLKDNLGILQQGQSSFFQVQDKLIIPNISFEQDLINPLDMSDGMERSVLKIQFLDFEQKWGLKQDISIQKQSNGDQQLNSQNSEVLSSQKSLKRSRSSQKNVFSSKLQINQTKKNQQNLSNSEDEDYIDNQQEESKLKKKYEKLSERNPFEDPYYVKLVDLHGFERLQYELDAYHDDKQFLQLRKKLVFYSLFIPILCKKIVQSVFFKICMSFLILFNVTLYIYSQTKQDSLSTKNMEKSIMICFLIELCLRIIASGIIIPKGAFFRNFQDIMDCILVIVYVLNLTYPETFIVDVSPLRVITLLLYLGDIFSGLGVMLKALKQSFRFLLEALMIVGLFSVFFAICGVFLFQGLFNYRCQYDNGDETDGWIQCNQNSCFEEGMSCQYSSFTPKLPTSFNNVIYSYGQVLRTITMDDWSWVMFFTMRIYHPWTWLYYLLIIFVCGFFGFNLVIAVIKIHYAEATEENAQEEERKKIEQKIKENREIPERDIVNVFDVAFLRYRDFFPVIQKYRQALRTTYRNIPLEEINNDKGQNNKARILSAKQKKNEEQIEDINWIKSIWISIQNFTIKNVLLPKFLILSEKQKRIHVKKYTEDEIELQILERLKSFQFSQMQSCVNKQIQQTFTSEGDVLPTLNLPDCEKKEIEKEILNMRYLKLPIVYPDIKETKIQQIFKDHKQTCQFPVHKVKRIILNFTENNDNETNNSIEQRQINQKLSMKQISKQKSSQNDQQIPFVIKKQDQYFVYIQGYYLNYEGVQLKINAKIQKNRINQSSNEFQYRLLRKKDIQQALINKKTWSGNDVLKLNEKRLFNFRDILKRLNIIDIQIWMLGIYGKIDTIKKYSYILITSQICQLFFDFVILINFTFLSLQGIANSVTISNEEDVSTIFLCTELILRFIAFSWKDIANSPDYVLQSCIVILNFIELTMSSLMTSLSEQNLRLIRGTKCLLFYRVLKYNKMAVAIGHIAQKTFKQYIYLTFLMFLVIFIYAMIGMEMYAGVFDQTDALGQLHSYDNILKAFMTIFNIMTNDDWYGVYVIGGNINYVFAVIYSYSMVIVLNYITYGLVLAILLDGFGAINKEMNEQEESIEKDEEKNNEQQEDQNSQITEEQEIQEFQLNLKPLISQGHLSDNQPKRTKQTPITNLIKSIKQVHKDILKQYPQLYEGIQCQQSLYIFSKENYVRIVCCKIVTSKFFNYFIDLVLYFSIIVFALKTYNDYEENSEIYPEVLQFLANILFLFEIVIGSIAKGMWMNKGSYITYTWQIIDVIYIVSYFYHFLSNGEKKPIVNFFLYFGYFRVMKLMYRLSWLDKLRLALGRSLADIWNVLITMLSVWIIFGVYGIILYEQQFGYCEDKVEFNVNKKECLEQNRTWINYKHNFDNITIAIPTLFVVSTFDGWGEILQIAENSQSSDFGPVAFNSYIPTYFFFLVFCFIGSMFFLSLFTGVLFANLKANQSKIERSDITKAQVEFMEISQIIIKDFPIYSSPPTSTIRRFASDLTNNSTIQKTMFCLLLIDLIVLLLFYSEMDEAFFQILNNTHNALMYLYIMWNLFLFLALGVNRYFDNHWRRFYFFLITIAIIDIIADYQSDWALVYFKSSPSDEGYQFLRLFFALRCLRIILIFQGLINLQRLMRVMVFAMPFLGKIFCILIITMFIFALFGCQMYGHLEKGQVMDDQINFQNIAQAMLALFKCASGDDWRTIMTDTMFYNPYCVEDDQYCGSIYNQIYFFLFMLLSNYVLLNLFVLGLVEQFEQFFMLQNSMIQTYVENVDKIKTIWCKYSSETQGQAMHYKFLCRFLLDIGKPLGGGNDENLWDVGKLASSFKIQCDHYGYIQYNQLMYELFRVCYHEEVFKNGPLSSIKKIKQFNKEMQLRLMYYRRNRYLQRTNISPILHLKANFNILHDYLTVLILYKAWEAYSKKLIKKVCIKQQHFTEGDLDEKDSSEDQKATNNNFLDQDCNEFLDEEIEQQIISNDNPQQEIKKHQITNCSEDLQSENIPTYRWQRDLQVDQVFESAIISPNQSYRKMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q647</accession>
    <name>A0A8S1Q647_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1450009</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Ole e I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="IGQSKEF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006041">
        <property type="entry name" value="Pollen_Ole_e1_allergen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31614:SF5">
        <property type="entry name" value="ALLERGEN-LIKE PROTEIN BRSN20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31614">
        <property type="entry name" value="PROTEIN DOWNSTREAM OF FLC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035884908" evidence="4">
        <location>
            <begin position="22"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="709"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="710"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="730"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8110634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1923" mass="212932" checksum="BE0D7D1E23871380" modified="2022-10-12" version="1">MLKSLLLVLLLVLVTLQKHIGNQIIELDEVNYQVIIKVIDSSGYALSGVIVNVKNLNNIIGEKDQMTDGTGIVTFTGKITKQTSYQITAIASTTFNDITTSGKIDGVGDSKSIIVFNGKLQFPQSDVQVQDVFQRGLIILKTQSNSKPLPGVNVKFTAIYSEDKKTKEINFKATTDEKGEQYVRFLLPSQKPAFIILYTASKNEYWNTLQMNHNAQEFALSPVKKYELPITINLDPGQFKFIITALVQDQTSKPLSGAKLQIGGMNLGSNKELESLNAASGTDGKISLTLPLKATDKIHVKEQITKDEYIGQSKEFDELFDLNVRKEQFSHDLGIIILQEASGDLQISGIITDSSKQVQFVQGAKITFTILDSIEPVSAITDAQGKFKAEIYVPANKDLQLKATVNAKGFMNKIAAPRQINVSSKQPLQIIFDVVLDRTIKNIKIKDKLLDPSGKPIQNAIVKIVESNPTMPDKTLYNKEIIDNADGSFEYPFSCYEDIKFQATFVFQVPQFVPVTYKTGDLECKDQDLVKYSMTLSKQTAILLIKGQVIDVDSKPMKGIALKFKSDPVLDVPNPNLITDANGNWQIEKLTVIPTTTYKFTIEYTNDIKQLVQVIQTFTPTMDKEQTFNFPVIGFQKYVKAKIAGKIVPGDGKAAIPLPLVITCDGKGKDGKPIVIETTTKEDGSYSVDVEVLVGADKPLSCVVTNGNNSSGGTGAGSGSTGAGTGAGTGTSSSSSTNTASPVQPIKVDVKVSAPTWSSNTNIPVTYNTVDIILKGVVSDKTKTITTVEGVDITLIITPQDVYVQNPGKHLLESYILFKRHFQGTTQSLLQTKSGKDGSYEFKFKAVQAYPLKFEIQAKKDPLFSPYEKKGIEEKCLKSETLTENIEIDRIRMLTKLHSILSGTDKKPIINASVKMTSSDPIMKDSKYYNLPVKDDDKGSFFLDFECYKEFDYVVDLQIDVPQYVQQNIKSSKFKCSQALTELNPITLILEKIQIKAILSGKVIDPHGDGVPNLSLNIITDPESANLETKTQTDGTWTITDPKIYPNTEYKAIITYIDINKQKQKIEQKFTIQSDNQKVPIPDINYQSVVNAKIQGKIDLEKGTLSQPVKINLTCPKFKDSKGADINQDFSTDNNGNFDIPLQILAKHDDKIECILKTKDSLQETTQKVELVAPKFQVQGVSIKSKLVTTTFTISGKLIDNQKLEDKLPGLKITLTLKPSDPISNQPIINKSINDGTYSIIFEVVRGVNYEATINIDAQPQFNIINEKLQLIGKEDKQSISKDITLIRSIIDCTISGQLVDMNQKPISNSKIEILSSDPQMTNTQLYNKFVTAPKGDFQLPFQCYNSVSTTLKMDMESDVYPKTALSPQVFTCGEKDLKLKPIVVSTKSVQITVFGVLTDSSGVTKNVQNAQIEIILNPADPLASNLQTKSDLNGKYSLSFGAVQDQIYTAVISITNSDFNPYKSSNIQIKADKVQSISHDATMDRIILQATLTSTITDPSGKTPPASTLTIESLQPTVKGNEKTQITGKTDSAGKFSLQVPCYKNVDESFILDVKADKYRDVKSDKIQFSCSGPAISLPPVKIKYELAPTNTKLTVGGEIIGPKGKGQSGVNVKLTSDPVTKELEAKTGSDGKWSILDDQLQFETKYQLTESFNDASGQNHKVTQAFQTNNEKSEMNFPKQYYDDFVPFEIDGKITSEGGIFPSNVPVSLQCDAFGENKVPIDLKTNTDSTGAYQFKWKMLMNANSNVDCLLISHETAKFQQTNSRFKINPNSDVVKITNQAQVTEQFKAQLTKYYQYSIINGAVIAEFDCNSKLEWKGLEEVNIKLKQWNGISYADLKIETKSDKDGLFVIKHQILKSQIQNEMRLEFTKQNYIPTTAEFNLYSFDPQLDGSYLIQLSNIRMVKIGQPDKCPSIKKNKHHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9H7</accession>
    <name>A0A8S1Q9H7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1480081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9H7"/>
    <dbReference type="OMA" id="NNDYHIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="285" mass="31144" checksum="3FDDA17DC49C3348" modified="2022-10-12" version="1">MSITVSTCQQICGSPLNNSTAKNLWTFSKSDRFGSLANPVNCGKAFYDLPTQIEKRSAGIGKGTKTDFTKVAFTTPSPQQYNITSDVEINQKKAKGNKFGMSREKMASTGILGNLNSKTPAPGTYDLGSTLSDIRYTMRQRPKTNFMVLTGKEIPGPGTYESLPAVNPVGKYPISKYNNSCATLFNPKNSKRFVKDFSTNLYAPGPGTYPVDKTGIQKDGHYFISKFHSSNVRSFPKESRRTGSVGKAGTPAPGSYRLPSEFGYYESRNKANRSAGDMGENKPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBX6</accession>
    <name>A0A8S1QBX6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1540012</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBX6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF203">
        <property type="entry name" value="PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="189"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="355"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="504"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="386"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8113056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="553" mass="64706" checksum="78BE1479C1C930E3" modified="2022-10-12" version="1">MYLKHIPIFIQDLGMYELSCLNKQELMYLYTKVAFWYPKLKTQFVTNHTQKISKQYFGLKINEQEEYEKTQTIGKLPQKDLPAPRVLIHMHIFKYQQWTLMAVRRNGLVNGINPYQVLSWIYTLFDIVIAYFFAFQFEETGMRELQLILLTVIVTCVIYSCLRTTLIDATDSIVKEEKLSKLLGKEFKTDIKSYCLVCQAHVQEKTKHCWSCNKCVSKFDHHCIWLNNCVGDQNYSYFFVLVLSLVAFKIFRLGQDCKLLYLQTNFQILVYICISVDPPVFFVLAYLLSIHLYFKWNNISTYEYIKSKKISKNKMMKTQNVQQKQIQNESTTGYGQLLSTSKRFDMKLQLSLKTGDQKKSNPNYFSNKQEEDKKTNQQTQQQFTQIPSLFTSKPTTPGNDQDKNQKISQLSKPKDLDDLKDKQIYNQVIIEDSEPQLNESSDDDDDEDHQINNRNHNHCPHNSHTSDLPNNFEQVNIQVNQLNEKDMGIKQEIDIIPNQESINQDSVSNQNSLQAKEPNPSQQSSKLALELHDNNNIIMQINSQKYISDHCIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QCM5</accession>
    <name>A0A8S1QCM5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1520096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QCM5"/>
    <dbReference type="OMA" id="GHRETSH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="422" mass="49821" checksum="0F0E15CE0E0BFA0C" modified="2022-10-12" version="1">MMRLNITRLTKRINMKPPSENDFSTLLIAKNTPIRQLSISTRSSKHSQFVRRLQTNQNQQSQQLTSRTISVHDTNLHGEPLKEKQLSVNKMQNKKKSSRRLIGLQTQPSLIMKPKFADIKYEMIDIYQLQIAEPKILYKGHRETSHLQKIAIIGQSISLNKQLIQKIKSECILVLLTNNLSQIDEQYDCIIQPPHISPKDVLSFQRNMNQIWINVNHLIQIYKQPQQIIMIDEIFNDNNILGIEELKNKILYSGNCKMIHLFLQKDKPNLQKLYYALCHLLKYEQQDLIQLNLKEILQECSENCLQIQTKLNYLQKVGDIKKKLKLQFKKDQISIESPEDRAENLRDCVIEYIKSRSTNKITGMYYIIGDQIMRNINFFRNRQEDKYFTDKKHQETLHILQNTLQPIKHALTCQQQYAFYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QD43</accession>
    <name>A0A8S1QD43_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1550007</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000089">
        <property type="entry name" value="Cyclin, N-terminal domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="7">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="7">
        <location>
            <begin position="109"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8113333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="448" mass="52456" checksum="35BA04EDE479EE68" modified="2022-10-12" version="1">MAQQKCKNKINHRQRQGDWICSNCNNMNFAFRDTCNRCQTLKNQKDNENKGFKSALFLTESNGDIPPISDRSNKSSGEIKDNGNNKFSFDKLPSMEPILKQITKETQQKYDLFEFEWQCQKCEKINQYYKIHCAECGAQRYRKLTKGVKTKDKQYNKINNFLLYQQNLTVKIMKDMSKKIFEKENQAEVSNNPRHQNKSLTQLVSLTQIGGNRKYRIQKQQFYIYISSRPLVFCKPEHKLKTQTPSSPQKQSPIKPKQVETQSNELCKLINNYSEPILDYYIELNNLTPKNCLSNHTVSANLRAKMVDWMVEVLTSYKCKDQTFFFAVKLMDTYLSKTQQKHIPQDLHLIGVTTMFMSCKYEEIYPVKLQIVHEKIAHKKLTKDEIKDKETNILSTLDFNLVGITVLDVITIVLSILNMNQQLYQITLYLAKMVXYKNKESSKICQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QD51</accession>
    <name>A0A8S1QD51_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1550012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000160">
        <property type="protein sequence ID" value="CAD8113343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QD51"/>
    <dbReference type="OMA" id="PYFEISC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003801">
        <property type="entry name" value="Ras-related protein RabY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8113343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="205" mass="23621" checksum="23E4EA6B5B8B6CD7" modified="2022-10-12" version="1">MSVQQEVIKYMLVGSNMVGKTSFLSRFCENNFKSNYEESKGLDFKSKIYNDGKYRLCIFDTPGAENLRYASYGFYKSAFGFILIFDLTRPYTLTDLRDDMTEIARHAPEYAQLIIVGNKSDQYDHHYYKESQEEAQKMANELNIPYFEISCLTGQNVDLVIEDLTTRVGNQMKNGNLSNQSGKQTQNREKDEQIKTEGGCCCKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF06</accession>
    <name>A0A8S1QF06_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1600050</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF06"/>
    <dbReference type="OMA" id="WQRYINE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16494">
        <property type="entry name" value="RING-CH-C4HC3_ZSWM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039903">
        <property type="entry name" value="Zswim2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21540:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZSWIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21540">
        <property type="entry name" value="RING FINGER AND SWIM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="8">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="150"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="153"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="7">
        <location>
            <begin position="226"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="321"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8114519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="54790" checksum="487AA6E24ECF85A3" modified="2022-10-12" version="1">MKNQTKVKTSKNPPQIILDLIEQAKQTRLYLLKRIGPTSLKYCDEDGNKFKVELSTTMICSCNQNNQHCIHTIHALLKVFQVDEKCPILWQQQYSQYDITNILNGQYKTKSQQTKTRQQISVIRQQQQQVQQNNTNSEDQQQNRQQVTEDDMCPICQESLVCNEALVHCKKQCGNNFHAKCMKVWVMQKQTAGKKINCPMCRVDWGDTALVEIRQEEKIFENLHKTHNKICSYCKITPIIGTIYTCISCPNYHLCDKCHNKKTHQQHIFMFKHTRLEKYEFEVNVTVESLIYLFPENLNAGNHETGGLMLVGTGFQKQSKCSFCHKQKQLRLLQCGHIADEGCINKMKEHCYICPIDNQPQFIGLFNNLSQEEKTLHLTETKKWIIQKEDEHNNQNHKLPPIGKHFSARPKQLSQKHFDNSDNKQQMKIQQLKQQQQYPIKRQLSANRKQLDNPLFIKSVRSQMENII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF23</accession>
    <name>A0A8S1QF23_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1570033</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000162">
        <property type="protein sequence ID" value="CAD8113874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF23"/>
    <dbReference type="OMA" id="QYLAMFL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="390"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8113874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="455" mass="53648" checksum="B81C05794B08F1BE" modified="2022-10-12" version="1">MNQQQSQSSTHRRNNSDISIDAQPVESESIMNLQIAYSWLTLALLIVILFNCFLILLLSSFAFNLSFRIPILFISIGHLLYLIKICITYNKISKSNQECCFELMWGVGLISYYSCLIYFIDNNDFQMQYLAMFLVFMTIIWMIQCIFQIYRHNTNDYQQKLIVVMIRFCFVTQLMFINLKVINWLPIKWIYTFSIFWLFLLIIILMQLVSLFKLSIIINQYFNCNIDQRQLLMIKIIGIIWTNLIIFGFCGVPTYALLKLSFYLEQNIEHDYVYEIITNVFYTLIFLVYTIYHKDLLSTFILTILKLQTQQEIIREQEIISFPSSRNQKKFLYQFRDNILNDFPKQLIKISSTYYLPEDQRNQTEINSAGGIYNKSSTLTEVADGSMKQCFNCFQQQSNTVYIPCGHGGVCSKCAFDWFKERKECLICRSQIQAILKIVQADDQRVKVIDIIALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QSG3</accession>
    <name>A0A8S1QSG3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2090004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000218">
        <property type="protein sequence ID" value="CAD8117764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QSG3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="268"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="469" mass="56051" checksum="FDC2067401D0F035" modified="2022-10-12" version="1">MNILTSQIQQQKPEILEMVCQIHKREIVAVDLDTQKKVNYNICAVIVQQRNLTITKFQLLIRQKKEFNLLRPKNKKIRQNRFNKGWITSRKYLIKSWNSNIYSQIKAQIFTIQQEKQSLLENSQILHNFQEDVKLLSEFLSTDNQDNQMEFQQDSQFIDEIFKQFELLFNYASCFQSIDIFKDAKQKINEINENVKIEIIPLQNKNKQKTPSLNRVCPTHNKEIIMIDIDDKNKKMEDRFVCVDCIDDHPKCEYRTIEKVNQEWNHTKNQQDRILNELKLKRQEKQEKLNQQIAIMRKNYNQQLNEISEKLITKFSRPINKTSEIGLSQEELVQNLNYLIQYDKENLTQDSKIEYVKSKDSLFSKEIESRLEHLKQHDQLDIQESINILQDIQNDNQIQGIVLLSQQLQENTRVNQEQMIVRQELDELINSSKQLIARWVYLIKLFKNFSNTLLKLMLLTTNLNLFQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QUY2</accession>
    <name>A0A8S1QUY2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T2820001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000291">
        <property type="protein sequence ID" value="CAD8119062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8119062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="100" mass="11150" checksum="A6C3DC610D18B60C" modified="2022-10-12" version="1" fragment="single">MQFKYKLGQFKWSIINQLPMVNIKTHELNQLDGLSGTVQSQCFQLDCTTFASGDKSIRQRDFQTGQTKAKFEGYNGRVLPACFSPDGNILASGSDDKSXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMD1</accession>
    <name>A0A8S1JMD1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMD1"/>
    <dbReference type="OMA" id="FLNQQYD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8042851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="250" mass="30341" checksum="F6F2156FA13297F7" modified="2022-10-12" version="1">MNNINELTTYRSRSHYSPSQLSSSSGFLNQQYDEFVNKIYGKTRQIKIHHLIPKQTNKVFEIQTPQFEDAIYKKMDDYIKNEKLSRRETRIQERIIEKEVIQQYKERQHPFKLLIPDQSYIKILQSCQNYMPKFKFARFNSPNNQENNEKLLLKVSELLQQRKQRSDIYKKQLEYERKRNKKILRQNFSTEKTQRVPSPLKLKNIEQIIQKCKEQSEGCSSEIKEINKNKYQLNKNYQLIKDQYNDIFNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JME1</accession>
    <name>A0A8S1JME1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JME1"/>
    <dbReference type="OMA" id="CYRIQQH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF40">
        <property type="entry name" value="POLY(A) RNA POLYMERASE GLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="1">
        <location>
            <begin position="31"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="351" mass="41250" checksum="BA32F5D57B5E15EA" modified="2022-10-12" version="1">MIYEQQKTSQSFEIFLENAIRIDQQIYMEDTLQALGQTISLANLKGNLYPFGSFCNGFQGTNSDLDCVFITDSDRDVQTILRNLRSAVKQYNKIKQTPQLRFVDLILYAKVPLIKILDITNNVAIDLSINNINGVLNSKLLNEYSQMHPKIQQLGQLLKLWGKNQRLIVTGQLTSYAILLTMIHFLQCKYDVPYLSDINLTNEQLSALENFGVQPFFNLGVKPNLERLSNTTLQQLLFEYFQYYEPNGEFEKKQICVSIHSFQRPHKDNKKRTLKIIDPIDPRIDPSKNIKSRCYRIQQHFLRAKELISNHQNQFLYQQQAVKNGNNRQGYQYYDKREKQYKKKQFRKRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN14</accession>
    <name>A0A8S1JN14_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0040003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JN14"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8042789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="47" mass="5696" checksum="1413B84995CBEDB1" modified="2022-10-12" version="1">MAFLIKKELGIMIQLFMLNQIKKITFMRLNKNFSNKPNDHRISRLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPN8</accession>
    <name>A0A8S1JPN8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0060143</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPN8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="76" mass="9158" checksum="395235E14D99C9A6" modified="2022-10-12" version="1">MKLLHNQSHKILIKNYQQLAKLISNFLQRTIKCEKAIGISKKLRAKNIQIRFRQEWYNAKIVVVKEERKQVKEPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPY7</accession>
    <name>A0A8S1JPY7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0050252</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPY7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949:SF2">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="420"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8043599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="506" mass="60994" checksum="E5F36FA3839FD3AA" modified="2022-10-12" version="1">MESIILGIKQRDLKYFVDSIDLQTLIPYKIHTVKIFFTNQGIFGIQNYQYSFRSQSIIKYHQKCLEQINKNQFQITVNTYIIQLTWYQNEIGINRLDIQTNKQQIQIRQKDGEKKEFKVEQNYQLGAIGGGYKYQLQFLEWQLIPLVEQQTQYQSQLYQLYLSQNESNQKSSKFEYVGKQYRVCDPQIIHQLINLFNFVQQIIQCKKSQEDFKMPGVYIPQLMNKSTFEDYSSEHIENIRKAIEYFLIKLSKITYFAQTVEFNVFITKTNKDSNQEIDYVQNKLKEMTQLTNIEQIDLRYKQNFEEFETKESVNEQQRQKCNDFSLLMSKLEQIKVNDFQDIKKLFEQHSKNVNYLSQYILPELHLLHLNLQSEEVIQRRKSNSIDRGLQMQIDTMNDVYDYFVTEQREVSALSYCMNYIKDIEQQKNKLEQKIMSQKLNQEELNTSKIQFQSVSSIWSILVNSYANYYIDNYFKERYQLYLLMINRLAQIQINYLKLRQNFWQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQL8</accession>
    <name>A0A8S1JQL8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQL8"/>
    <dbReference type="OMA" id="PNSKEDM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="73"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="233" mass="26423" checksum="3282E3BAB52E6E16" modified="2022-10-12" version="1">MAHQLGVIQETYQDDLQELISKLTTQNQIIDKRGPILKKRRELLLGALKDFREGETKLCDSKISCSTALFSSIHDDTDQIQKLQDEIALKDQQIQELKDQVESLQQKIHRIQTCNDEMQFELQKQQQQQQTFQRGNKLSQSMIVPTLTSSTEKAQATISKFVQPISTNSFKSIDSKEPNSKEDMKKMIRRISQQASASAYILAAKGDYTTLAAAQEELQSQKSIGSQKSLSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRC6</accession>
    <name>A0A8S1JRC6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRC6"/>
    <dbReference type="OMA" id="QMVYINE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="875" mass="105421" checksum="40FC698A71AA59DA" modified="2022-10-12" version="1">MNIQLAQKVVNHWRSFVDKRKEVRSILIAQYKQSYIRVLNSYKELKKRRFQQYIINNASNLIYKALKINLWKVKHLRQFLNQNKVKRFYIQKSQEQIFQKTFNLQAKEVNIKTIRLHIQNSCSHKNLIPLLFHNIKSAVILFNFHQLKKTFGETNIDKDVIKYEFLYNFIKYVKVKLSKKQFELTNQELMRHKNLLQKLDKFQLEEYNLLKYIELDNLNILKKIYQYNSQNRNGQLHIFFDFQLREIAAVCRIQKYFRAKKNISQKGMKYQVIMKLINQSRAVYVIQRWFRRIRWNHRNNFFKEISFYVSQIPTPNLYLDMETYSKIQAIVNNQQNSLKFLEQYNMIVADNETVRLQFKSNNFEDQVQSTYVQSLNLFNHLNVPIFPNWLIKEIQMVYINEEKLIDYSIIKQEQISTKWNCRNREYLSKSKESLKKLKYEKPVDLYGLLHFGASISFDYINNRDYIKFTYISTSEAKYRIIALALITYRFKFNGTSILMLGDNIWESTHQQQTIFLNKYYQKIQNVYKIKIPILETSQLYKGKQNQVILCLNNQQAQKFKFDKQTIQLQFFNQKFRIIENFIWMCPELISQQKYLRNQVSIQLNQNSLQESSHIQQVNSSFGDIQQDYKSFIQNFIQQKSIDISPQSGIIDGSSKSTQLPVKFSIMCLENQDIKFQQPKFKRRTEQSNQEFVMTSRFRTQNNSLNNSKIKEQNDSQNYSVIQSKKQQFQLEDINEHIEYSQLKQQQQDITLIENKEKITKIKQKNINEMNFIREFQNFTSLLQRQEQKIMRREIKISRNKTLEKSQKKGSVQQTIKSQSITRREDYQDVSIVFPKIEPKCTKKQTINSRLSNYHRYAVFPEELYAISSRKETLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTW0</accession>
    <name>A0A8S1JTW0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0050410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTW0"/>
    <dbReference type="OMA" id="XVIAQFW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035870229" description="Beta/gamma crystallin 'Greek key' domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="2">
        <location>
            <begin position="981"/>
            <end position="1054"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1067" mass="118333" checksum="3C9554F07866DE95" modified="2022-10-12" version="1">MRFIVIAIFVMLATSQEFVEFYSAFSNADLGQLDGWTIQGSLSKAQADNFFTCSDATIFGGPKAFGKGAIVTKQFQLPPHFQIKISVEVWKFDAWDTQRQFFYLDNYLWETMWTGSDGTKRCATPAGGNVNSFQVDQTITHNHAALFIAFASSADKSADKMSWGFRNLKLSYLACPTECGTCLGPDAIVCQMWVPVSSSWIKNVEADGWNVKGSSNVIKTSTCAGIPIVGGPGNFGNNVSIQKIFDKLVPHYRIKVIAQFWKIDNWDNDNAVLYIDNQEKWKQNVAQVEDKEFFICDKITPGGVDGTEKIINVNFESPHNYGAALVKWTATTKKSSKQASWGIRQFQLYVAQCSSNCATCTGPSAEDCTACVSPYILLDPEDGNGSGCILKGDWVEFTSEFQGSSWTANEDWKVSDGKNGPDFTSTCGEFKFFGGYEKTGKDAKISRKFNLPAHKRIRIAFEAWKIDNWNGEDYIVKVDGLSVWQRTFGFGDPGLADICGGDGFENYAIVDFVMGHEASTLDLVISTTIGKNNENASFALKGFKLFYEKPDACATLYTNSFQVDQTITHNHAALFIAFASSADKSADKMSWGFRNLKLSYLPCPAECGTCLGPDAIVCQMWVPVSSSWIKTIEADGWSAKGSSNVIKTSTCAGIPIVGGPGNFGNNVSIQKIFDKLVPHYRIKVIAQFWKIDNWDNDNAVLYIDNQEKWKQNVAQVEDKEFFICDKITPGGVDGTEKIINVNFESPHNYGAALVKWTATTKKSSKQASWGIRQFQLYVAQCSSNCATCTGPSAEDCTACVSPYILLDPEDGNGSGCVLKGDWVEFTSEFQGSSWTANEDWKVSDGKNGPDFTSTCGEFKFFGGYEKTGKDAKISRKFNLPAHKRIRIAFEAWKIDNWNGEDYIVKVDGLSVWQRTFGFGDPGLADICGGDGFENYAIVDFVMGHEASTLDLVISTTIGKNNENASFALKGFKLFYEKPDACATLYTECNYTGKSFNMCEDLPNFQLAKYPSKIKSIQVPAGAKVILFDEMDYNGKTIEYTENQKCIEEYQYKFLQKNQQNYVLTANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWB9</accession>
    <name>A0A8S1JWB9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0110316</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWB9"/>
    <dbReference type="OMA" id="ELHLTQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="168" mass="19297" checksum="D631DF6E821941C0" modified="2022-10-12" version="1">MGSYPSCLCCPQSYSQGKQNTIIDNELHLTQFKQPLFNQVVQITPEDDSSLLQIMATYEQLVNRKSISTAYNCSSNNRSRSISVDFKNSGVLISKKHEKKKKEQYCRSFSAVQFQNNSSKLSTTQKPSSILKKRSNTQESQFQKKSKYKVQFLPFILQCNSKKLIDIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWC1</accession>
    <name>A0A8S1JWC1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0110179</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWC1"/>
    <dbReference type="OMA" id="QLRLGCN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000819">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="280"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8046942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="364" mass="41375" checksum="3A891FAABFC3050D" modified="2022-10-12" version="1">MGGCEFKFRGSNDDTQMALQNFELISVVGQGGFGKVYKARLNKTRNLVVALKVMSKVKVIQKKSVSSVMNELQIISTLRHEFIINIISAFQDRCSLYLAMDFLAGGDLRFHLCKNRKFSEPITKHIAICIIIGLDYIHSNGIIHRDIKPENLVFDNQGYLRITDFGIARIWKPQNSHETSGTPGYMAPEVMCRQNHGVAVDYFALGIIIHECILGKRPYNGKSRQEIRDQIIAKQAAITEVPQGWSNAAIAFANALMQRKPQLRLGCNGPDEVKSHPWFKDINWKDYEKKLINSPFIPDGKLENYLKSNQLDSLEDKSILHSEQVQQQFLGYEHFPQIDGQSPFLSSIVSPRTPNLRIQLKKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXV1</accession>
    <name>A0A8S1JXV1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0090460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXV1"/>
    <dbReference type="OMA" id="GDWLNDN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8045520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="39503" checksum="CCFF5A2B9E74A584" modified="2022-10-12" version="1">MGQCKCATACYQKEEVNSLKISSHSIQISIKQNKNYQDKTSDKIEPPNQKDLDSGILKNSINSKISIQELSKNLIQTQARMPDQVIDTLQSSERKTLPLMQLPNGTKYEGQWLNGMRDGFGKQIWPDGSIYEGQWKQDKSNGHGKLIHSDGDIYDGEWIEDTACGKGIYIHINGAKYEGDWLNDNQHGKGFESWPDGARYQGDYKFGKKNGQGILTFTDGANYEGSFVDNEISGFGTYRWADGRIYIGYWQDNKMHGEGTLKWPDGRSFKGIYENDKKQGNGIFYFGDGRKYIGTWDDGKQNGLGIYYQDEQQYKIGIWQNGQRKKWLNDQEIENVKDSISNLQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JYM2</accession>
    <name>A0A8S1JYM2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase S11 D-alanyl-D-alanine carboxypeptidase A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JYM2"/>
    <dbReference type="OMA" id="RELYWEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581">
        <property type="entry name" value="D-ALANYL-D-ALANINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S11 D-alanyl-D-alanine carboxypeptidase A N-terminal" evidence="1">
        <location>
            <begin position="106"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="356" mass="40944" checksum="C60AA345EDC22CEA" modified="2022-10-12" version="1">MNDETLQPIQIKNWTQGFQSQLRSSAKRTLSRLKSIETNTISQDPSSEQPYKFKTNPKPIKLFDRGVISQYGSRRISFKQKQTTQITFQQQLCKAPLYMQLIPFFKVSASSWAIFNGQNGEYITGFNQNKCRQIASITKIIENAFLFQIKLINQTKQAQKISGTTAGLLKGDSIFLRDLLYGLMLPSGNDAAVTLSYNFQLYSLENDHFLKEMNRFVSSLGLSNTQFSNVHGLCNENNFSTAYDVGKFTYESLQNEQFQKIVRTQSYYCETPNQLQYNSRELYWENTNKLLQQGFKGVKTGITKEAGPSVVEYFQDQTSSYIIVLLNCSSSESRWTDAQKLLQWIQKQDTLIKQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3R5</accession>
    <name>A0A8S1K3R5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0140024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3R5"/>
    <dbReference type="OMA" id="RKDWQDE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8049507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="138" mass="16557" checksum="82C3B40DF8CC29EF" modified="2022-10-12" version="1">MNQQNKRKFNVLEYLKNPYHLPQKQEKFEIPQTCKLGSRTNQRRFYRPYKHKTLFPELNSTNALYLTKTSQKFRQSRKDWQDELQTCLQSQSIRGVPNKSKVSQIFVKSLHNSSFNQLLRKTQPELKPLLEIQAQKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4V9</accession>
    <name>A0A8S1K4V9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0120242</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4V9"/>
    <dbReference type="OMA" id="NRPSQYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13384">
        <property type="entry name" value="HTH_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8047933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="183" mass="21591" checksum="F65BE0323CF8FC93" modified="2022-10-12" version="1">MDNHFFKSEDNLGLQNSLENLKKSNRPSHYQKVDENIRQQIIDLACNKGQPLRQIAQNLGIKYSTAKAIVQVYQNEGRIGKKRTRDKRIFQEIETFIMVVHKESGKIEKLKHKSECSDLKSNFQSAKMNLEEVHYSQLAQYFERYNLQEEDKVQTSQNQTLLDIVKILNTQYQQFDNVPQQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K592</accession>
    <name>A0A8S1K592_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0140206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K592"/>
    <dbReference type="OMA" id="PNKSMII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8049871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="132" mass="15705" checksum="BD308693D30FF843" modified="2022-10-12" version="1">MKRFYYEYYQQINNRKKNLNNKSELFIGRRENEGSAIHSMEQIKSRKFSISRPISSISLKRNSTQQIDSSFNLISLNELALIQQYKTVNLLSNQLKRSSSNQFRRRVISSDKNLQFRQFPNKSMIITNFTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K5X9</accession>
    <name>A0A8S1K5X9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130329</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K5X9"/>
    <dbReference type="OMA" id="KNFNDER"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8049215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="241" mass="29206" checksum="ADFDD9CD23677539" modified="2022-10-12" version="1">MSQQFYNKYRHLFEDEMFDKMKHGYDMQIAPPQQQIPQFKFQQQQPKIQPQQSPIIQKSPFKIEQSYPQQIQPQVQSFQTSLQERSTQSRKNFNDERIRKQVMQDNIFKQPTLEQRQGDRIYQQNQPHQVQHQEQPLQERKQQLQTYNVFQDQDILDDYAPKKQKQLVQTTNENSQINNIHKVYLQRLNDLDYIRKKYLDPKQLARDIDQNNPYKAALMQRILAQMNGNQRKSRLKDALKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K830</accession>
    <name>A0A8S1K830_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K830"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8049206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="42107" checksum="A7FC0813EA475F31" modified="2022-10-12" version="1">MSIYCLCQEIKKPLEFSKITSDSNLRESSIGYNLITSFEHSQQCKAFGFNNSNSIWVMAYGVEILVCQNDNQTMRVEQSLQGHKSCVNNIVYSNKEDMFFSIGSWNDNVIVWTRDSDRKWNKQFLIYKEQQTRDFEVVQLILNNDENELIGVMDYNFIVVWQRSSENQWINKQVIQISQNEHSQILQVSYDKNCMKIIIINQSGQVQFLAKNQFNQWKKRQLINLKCIKGKKYYNIDNDQLVIQTQKMEYLLFNFNTKNELYEEGQVNMTQCLQSMLNQVFEYNSSISQSQDVVGLKRNEDLLIFTKDTKNIYQQIQTIQFEHSFFYQFSKNNQYLITWDYSKFIKIWKQNND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCD6</accession>
    <name>A0A8S1KCD6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCD6"/>
    <dbReference type="OMA" id="NYSFDAK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="230" mass="26885" checksum="FE06590E078AA7E0" modified="2022-10-12" version="1">MLKIEEYVCNLKESKTLFNDALRIDTHQSSESYDNTQFQNPIYNIVPPEYFQSVTENKYSNIFSSYIQSPKHEELQSTIKQAPKKRTFSFAESQQLNKDPLCNISADFGLNTQIPSKGDLKNYSKFSQESNLKNIETPNKDRFQLQDSMLVMNNSIPQSKLSSPQKKYVKVQKFKNTNYSFDAKQTQTTQITNISLKDNREFEQIFKECQNNFEKNKARKTEEYIACNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEL8</accession>
    <name>A0A8S1KEL8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0190066</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KEL8"/>
    <dbReference type="OMA" id="YENCSPC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012389">
        <property type="entry name" value="Cyclin_P/U"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF108">
        <property type="entry name" value="PROTEIN CNPPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027110">
        <property type="entry name" value="PREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR027110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8052205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="159" mass="18908" checksum="7109081DC2F9627E" modified="2022-10-12" version="1">MQQSNPILQIISNILDEIIKETDVLELESNSIFHAITAPAISIYNYLQRINKYTHCSEQCFVIALIYLDRLQEKHANLVLNSHCIHRFLLLAIVTAIKFQDDDYYKNEYYAKIGGINLKEINKLEQEFLEYMNYDLFIDEQQYQIYENRLLEYGEIEMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KG80</accession>
    <name>A0A8S1KG80_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0200055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8052895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KG80"/>
    <dbReference type="OMA" id="IQCKTMN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="35872" checksum="FC3F6557F053FE11" modified="2022-10-12" version="1">MNLIKQCQQSNISCICYSILVGYAIIITLIAIKAFSRLMKSSTSIQELIPMILCIVQSLLHLIQYAVTENNTLQIITLYFQILIFTSISFLFAKLCYQVKSGILILKNFKIFKILQFVFYGIILIFQIFALVFSSQDSCDRYYYFSIILIIVQQITSTIITTYFGINLLQKLKRVSQFIQCKTMNPKAVYDSYANMSLCNSRATIIEPRELIQTRKQIIIVIYLMIITILFYISFILIAILIFKKVTCQHQIDNNNQNYLILVYGIIQMTPCLVIPYAFGFIPQVQVQGDYNDCLIILDQSQAKSKDSIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHY5</accession>
    <name>A0A8S1KHY5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHY5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="80" mass="9581" checksum="6434E0C4A5426BD6" modified="2022-10-12" version="1">MSNVGKSTIINQLIQTTTNQIKEKQQQNQESYSLYCKKFNKNQQICDEHLLNLMNNLYLNILNITKEQQRLKQQRIIRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJU0</accession>
    <name>A0A8S1KJU0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200298</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJU0"/>
    <dbReference type="OMA" id="TSAANQW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="212" mass="23837" checksum="BE49DB69853D190B" modified="2022-10-12" version="1">MQSLVKQLLTLSNTSAANQWQQIETTLQPKQLTPAEIADVAHHFAVNNQGSELFWDTLQRATVDQLSNFNREQLATVFSAMMQTPYEVPDDVGEAVTKAIDTFFLAELQANQKSDPYYLSKFLLQFQSGLGQFALNFGPGFLTGRVNDEHPEQRLAKGDYDLSESCNSELRSAFIKGDLNEEQVKEEKTKFLNRVSELLGKNQNAQDVANLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KM70</accession>
    <name>A0A8S1KM70_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CENEMII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8054102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1770" mass="212315" checksum="C62997D4AA2AE40A" modified="2022-10-12" version="1">MKQALLKLRDNKLLEAFSYFEQHKPFEEAAQILIKYSLTQESNIKELIYRWILRNTNSPNIKLDVYNLLGCTLVNKDQQIEILNEALKLTVAHELNPSTTLINLTCIYSKLKQHEKALQIALQSIECSVGKQLVIAYHNAAIEYKALLQNEQSRIFHEKAYEQSVYLFGQQDPLTQKLSKYVEKLPPKIVPVQNKFRLRSASRQGEDAKSFYYRRPITIIDSHTQSLNQSINQSDDSNYRSSSQIKGRRQKTLNQSFQTSSNDINEEEGSVVSKQLQNEIEAQRKIRLRFLQSIHQQNIQLLERKLNKRNSYHINFSQESILNVECQDQRSQDFIHFENQQPDGQYDSMIPLNQQEDLTYDDQELIINQIRGFESQKEITTASFANQETFENTFEQKNKQGKAISKIQKWYRKKQKNHSVKYYSNNSQIKIQAAIKIQRFYRKITQQRLMQLMKNKKYVKYLKISKLSIENEYEYCLIIAKKFENEILYIINEMKNKRQRKKAKIYRQTFELEILNQKYRAFDEFCLTINMSKRMNQIAYKQAQQINYKEICNLFYVLNNQLNLYGVTDTLLNELIDSDIDDKVSVSSRRQPIKLTKLMKLQSLLRGYLARKKQELIKNQKYKHKRFIEKIQGGYFILIFQQENVIQFYNLQQKQLCENEMIIPDSVIKFFGPINRQNCSEIFYAQNNKIFFTDDAQYIIDKDIRSKEKMDFSVVEKAAKKIQRVFRSKIKFFDSSILKYKTITLKAKQSQLLFKIRKSILFEQKYHIILTAYGQIYQEHQELLIEGGIIQKKYSNLKIAQCCQRLFQDISISDFNQVSKIVLDEIDFEWKNDKLHLKCHSLEEVYGFLMERNYSSFKESLKDVANKEPTFLGRNYLSRKIYCQQYLKNRFEIEDRPTIFCRVEDPSIYQITYIKNLYLERLKKKYQQKKKEKQVGDNINHYVNQFPSIYKYEWIQDPDELYMDMLNKKAKLVQRAFRLLKFRAIIHQKCLIRRRRRLALLQSQKVVVKPYYFDEIRKPQAYTTIEIIQRRGETPIFLKSIYIDSVLYEWILILPYYDIIVTVYTIPRQFYQIKYNEYKNHYMDIILSESVEKIQQYFSQPIEKFHPSEKIDFITELREQFSKKVIRIVRLKLFLKKIKETPQTKKFLIRTFVNEFFVQINYFYNKVDQKIIILLQKEKRKRKYTLDLHYLDCLFGNQHGLSYLDKELFIKLLTLNEPKSYHKYRMLLMPMINVLHLYIMINAELDLQYQYKNFEMTLTEEINEKFYTSSQGFYMITNESDSKLLPTIISLQARWRLSQQQKKYQLMLFRCTRMKIKMMNKKGKEVQKTFLKRKDISDDKDSDIIYICSIYESKDNQGVRVRVELISYKSKQEQELFNCFTIDPQSILLNEQISQYVLKNLYIEDNILLLRQNYTQKFEIPQETQQKQLKLITKKPSSPKLIFHQKRQYSQGKQFIYDAIHTGEMPQQQKMTQETRNIIKIDIDLKELKSQYPGVNYKQSNILSQLSEQLISGLKVDKQAIISKSIQKRGIKIQGNCQNEEKGKELNISESTKQQSTLLSRFTQFYNNLRYMVVISLLDIPIDSYEKLDSVYEPYHSYINITAQNQLNNKRIIWQMNLQQANKITNQQDLKEIAQTIKFNILVLEDKFVYVADFAIIEFDNVLMIKRNTMNVIQRMLKNKKFNKLRKQFEIEIKQLKESDNQFWNNIIYYGINQFSSEDYFICIGIERRQYLLLAWNIKDYQRYTLTMYKKKGFQEQLDQIRFINQNLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KNZ3</accession>
    <name>A0A8S1KNZ3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0240321</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FTWISEP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000153">
        <property type="entry name" value="Dynein axonemal heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000005">
        <property type="entry name" value="Dynein axonemal heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000005">
        <property type="entry name" value="Dynein axonemal heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000007">
        <property type="entry name" value="Dynein axonemal heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000006">
        <property type="entry name" value="Dynein axonemal heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002141">
        <property type="entry name" value="Dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000002">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000001">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000049">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF68">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="171"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="505"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1116"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1322"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1646"/>
            <end position="1701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8056457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2543" mass="293306" checksum="CDB1DE5E19552991" modified="2022-10-12" version="1">MNPGYAGRTELPDNLKALFRPVVMVVPDSNIICEIMLMSQGFNSARVLAKKMTVLYKLGSEQLSKQYHYDFGLRALKSVLVMAGSLKREAADLPEDTVLMRALRDMNMPKFIFEDVPLFQGLITDLFPKIDVKRKPYEKKDKIQEIVEQLGYRPLDDQIDKVVQLFETMLTRHTTMVVGPTGSGKSAVIEILKRVESATYYCINPKSITVNELYGVMEMTTREWKDGILSKVFRIANEKPSGQQEVHQRWILLDGDVDAVWVENMNSVMDDNKLLTLINGDRIRLERFCKLLFEVFDLQYASPATISRCGMVYVDPKNLGYKPFYDKWLSKWQKKGDKAEGLKQNLEEFYTKYIPTLMNLIFEGIDGEEQGHVLEFSIPRTNLNCITQLTKMLDTIINEEDPQFEQENLELAYIFAIVWSLGACLKFDARKKFEEVLRRVAQRHIPPGSLFDLFFDYTLDNKAWVAWEKKVTEYQPPPDGKFSKILVPTVDTKRFSYLLGQNITHKQPCMFVGDSGTAKSVIITNYLNSLPSENYMKLNINFSSRTKSIDVQTALDENIDKRSGRIFGPKIAGKKLIIFIDDIHMPKVDIYGTQQPIAWLKFLIEKGFCYERSQNLDQKIIKDTQFVAAVLPPNVGANPLDPRFLSLFNCYQLLFPSNENLERIYNSILKSHLQGFPEEVSSTVAKITQATLQIYSAIVIQLPRTPVKFHYIFNLRDLSRIYEGLCRSTLDKFQTKESFIRLWRNELTRVFVDRLINDQDRDLINVDKVPSLIREHFSDTLEYAIQDPLLFGDYLTANPLDPDVVDPKLYEDCGGFEKVGQKFNSLLQDYNEEVKEMNLVLFKDALEHLTKIHRIIRFPLGHALLVGYGGSGKQSLTRLSAFTASYDIFQITLTRGYKEKEFREDLKTLYDLLTQKPTIFLFTDAHVLEEGFLELINNMLTIGMVPALFDEDGKKKMSDKVRDEAKRKGILETKDELWNYFLEKIRDNMHIVLCMSPAGDTLRIRCRNFPGLVSNTQINWFFPWPEEALVSVATEYLKDENLEDETFRPKIIQHITKVHESIQMFSRDFELQLRRKNFSTPKNYLDFLQNYKRLLAVNRKKYQELIVRYTNGVLKLDEASEQVKVLQGELEIKKVEVTNESREVEDLLKIINGKKEIATRDNEEASQKKKQLEKDSVEINQKQQEADQILKDAIPILEEAKEALSKIDSKELVELKALNSPPKPVGAVASMLLIFKPIDGIEGDGWNAARQMMNNPMKLLEQLQNYGNKIGKVTRNQIEKIKSAMKDPENRLDEISKISKAAGGLYKWVSATVNFYDVYKKVEPLKQRLEQMNKQKEETEKDLTNTAIKLAALQKEVSELQVQSDQKAARLAELTAQAQEMEKKLNAAKKLIEGLGGEKKRWTEDTGKLAQMTLQLIGDCLIASSFLSYVGPFDYSFRRRMLYDHWMVDIKEKELPMNPEFKFEDLLSSAVEISQWNSEGLPSDELSVQNGILTTRASRWPLCIDPQLQAVNWIKKREEKDIAFKVLNLNEGAGVFLKPLENCIRFGKPFLFENVDEELDPTIDPILEKNFIIKAGMKSIKLGENIIDYNDDFRLYFTTKLANPKYTPEIMSKTMVINYTVTLTGLRDQLLNVVVSFERPDKEKQRLELIQSMSENKKKLKEAEDDLLQRLSEAQGSLLDNVQLINTLDQTKAKSEEIQQAINDGQITSQEIEQARQSYTTVAKRGAILFFAMSSLSSISEMYEYSLSAYLQVFNQSLRDARKDTILESRLRNIIDKLTSNVYDYTCLGIFEIHKLMFAFQMTIMIQDGEKLLNHEELDFFLKGNTSLEQVQRQKPYEWVPDSGWKDIMRLMQLADTYRLLADDLEKHGKEWKKWYDHERPELETLPEGYTKLNSFQILLLLRVFRPDRVINGVKKYIQDIYGNSNYVQPPIINYEKIFDQSNEKSPIVFILSPGADPLPDVIKLGDSKGFTGAKFRNLSLGQGSEQEASQFVEAASQRGHWLMLANCHLLTGWLKSYLEKTLELMQKPHKDFRLWLTTQPTDRFPLGILQKSLKVVTEPPDGLKLNMKAIMSKIDENSLNSCPHVAYKTLVYVVSFFHSIILDRRKYGKIGWNVSYDFNESDFNISSSLLSMYLRKAYDAKDETIPWNSLKYLIGEAMYGGRVTDSYDRRVLITYLDEYMGDFLFDKNREFLFAQSEDFKYEIPKILNHEGFQTMIDHLPLINSPVVFGLHPNAEITYFTNSAKSIWDNLLQLRASGGAVSGGVDKDKYVNDVSEDVLSKLPVIWDVIALRKEAGEIISPTQVVLFQELERFNKLIIKINESLFNLKRALKGEIGMSSDLDELSLALFNGFLPGMWRRLAPQTEKKLGSWMNHFKRRVQQYTQWASKEEPYVMWLSGLHIPESYLTALIQTTCRAKTWALDKSRLYTQVTKKFKAKDINQRMEFGCYIEGLYLEGARWDVENNCLKKQNPKELIYEMPLIQVIPVEANKLKLKDTLATPVYVTQSRRNAMGVGLVFEADLRTQEHTSHWILQGVAMTLNVDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSB1</accession>
    <name>A0A8S1KSB1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0240168</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSB1"/>
    <dbReference type="OMA" id="DEVCQIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF3">
        <property type="entry name" value="RING FINGER PROTEIN 141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="102"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8056152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="175" mass="20762" checksum="217408A4ABDC73FD" modified="2022-10-12" version="1">MGQQQTKQPIHVLIKDIEYFNKVIKSMPLQQELNKQIGAELIQNKQTFQEQLLSFTAKTDLAIILKIDERQKLIDQETFYKLKQLIQDLAYLYMKNNDDEVCQICFEKKIDKLLPCGHSYCQNCIDGWFSVKLRDSCPMCRTRFTKQKMLQNSYCFPDEKDFIDSFKEEVLKLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVN2</accession>
    <name>A0A8S1KVN2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0250039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVN2"/>
    <dbReference type="OMA" id="PQNQEDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="212"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8056756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="371" mass="44743" checksum="9FB0D8392BEB3A9F" modified="2022-10-12" version="1">MKGGKQQKNKSIWDQVKNEERDFYYNFLAKKYRNLQKKLKDIADLEELQKTKELKQEQIQKIQNKEENNERIKELEGQLSNWLNAKKEAEQQGSIITIEAYIIILQNLAQDPQLVALLTQDHESTHELAKKLQSKIVGQSAKTDQPWKGLKFVIQTQEQHQETKQIQEVTETEPIQQQVQQVIQQQEQPIQSKQTPSEEVQLQSKQHEQPEIQETQEQQQFSEQQPQNEQPQEDIQNQVQQPNQDEQPQTKEGQRHHQHRDDRRNYKKNYNNNNYDRRNNYDRPYRGNKQYYQNRDRNYQQEQIWEEKVDRQAPQNQEEQQQEEDDYITVRSKKPQKPQRGGQRNYENRNNNRNYEGNQSRGQRNNEQVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KWC1</accession>
    <name>A0A8S1KWC1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KWC1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="157" mass="18343" checksum="9DA24696DA37C3A6" modified="2022-10-12" version="1">MILSGLVITLNTLTQNYIIFENQSMNPKFELGNYDINSCGVPQLQQVYNDNPQSNNLFIQELVISADQQFSLQYQVENVIDNDFTLNQSEIMNLTLSNRQVIYITHECPQDLENYNFWGLIRVDLQINDESIKIYYESICSNQYEPKKYFTSTTVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0L1</accession>
    <name>A0A8S1L0L1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0280113</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L0L1"/>
    <dbReference type="OMA" id="QNFFERN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="732"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="765"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8058933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="860" mass="101110" checksum="FC89EF1B34CFB9E1" modified="2022-10-12" version="1">MKPRNYSFKQETTQSEFANLMTKQQIQNRFTQISKQPLTRSTSRTHSLYVKQTQIQNMCTIFEKLINKSNQGKPLKQKYQNICSDFGIDFNILQQFTKLEEKQNYIIRCTVDEISNQFDRIIQKYDSTKSNIQNIDDKLDLYGWLKVKSQYKAILKRLKFALVQMIQNSPRVFFEKQCKNDNSELNNINQQTKFNLKNFDIQQPDVYFEWNKAVEAQFISMNKNYIQKGYETMKKQLANGTQTSKRGIILDKCEQTGNYITKEELQYIASTSFLINKNVDKEFIQRAINSLQNEIKQQIELNISEQILEDYYTLKENQMKKRQQYFEEQNVIKRKQIADKTINYKKKQYLSKDSLQMKVVKSKFVQYLIQAQKKKFIKEAIEIKKQSILRRLQKSGNPLANIYVQKFKEIMQNVVKRYRQKKGNQIVSQQNFFERNAQKQMIKPLFIKDGEEMPLEQVKHKFHPPSKLLISAQEDKQNQIQKEKLIKQSQNFRLAIRNYNIQKSEFLQFYSGGPQSRRRFQSQQLKFMNQIESKELPIQQGWVSEEIEIQAANKIKMAIKRYLYKKKVSNILGQKAVLKNRDIEIKIQKCLKAKRFFEELSHELSKRQTEKLKDLSVFSPPKLPLDQIQKSIFIPYSTPSSPQTQRLSQQKTLDISLASSRIVKQSLHQKQELKNESLKQSLINKVTKIRISGETKIKSRKLIQAAKMKSVTIAESAGFIYVKADTQQYDQYGNTPLYYSAKFGDIHMTKLLLKAGADVNQKCSNDNTPLHMAFQSGNKQIIIDFLNKGGDLNLINKDNCTPLAFGSLDLLKQLNLQDYIATINPNLQRRTDNQQLLHQKIISQNYQIDDELLLELKMRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0P1</accession>
    <name>A0A8S1L0P1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SIAH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0310224</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L0P1"/>
    <dbReference type="OMA" id="NDICPNK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051438">
        <property type="entry name" value="RNF_E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016:SF1">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016">
        <property type="entry name" value="ZINC FINGER, RING/FYVE/PHD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SIAH-type" evidence="5">
        <location>
            <begin position="77"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8061269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="405" mass="47301" checksum="E01D7061296FF638" modified="2022-10-12" version="1">MISREDFIIAQEIKDEYLCGICYQLAINAVECQKCETLFCNQCSLDWKKKNDICPNKCEKPFDLKQAHRMIRSEILKIKCYCKYTKFGCQEQLILDQILQHLQKCQFIPVKCCEQCEWEGQAKELENHQEKCNFILHITCKQCKQKVLKEQKETHNCFETLINLVINLQTEISEVKKSSQIKINDLELKLEQQSQLQTSIIKQSQILFEQQQQQINNISNIKSDKPPESIIVNSLNKSSYQQQQQQQQQQQQQQQQQFQKPPVLQKQIPIFKTCGKLGDISKDKMCKQKHLLRFWLTPGIEASRQTCSNCLKNQICRYVCETCSDYYCLICAPPENMCRGKSLPSYCPTGHRMEFRKGTNYVCDVCGQSGSDMPNSKTQCCQNCDFDICQNCQEQMIKNKECVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L173</accession>
    <name>A0A8S1L173_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0300257</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L173"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="294" mass="34062" checksum="ED71BEEA661872C4" modified="2022-10-12" version="1">MHLLYGDNIYTILSQREFFEQIQLRYRGEIETQTPTIKISYHQIAKLCGGIETQVQQTQKINDKQFVFTKQFGRSLIQAIVEGVFQSLIQKIKAKNQIEVNQGYFGKFLRFDFTFTYEPLLKSKVQSQIKQELQIKKTVRNLIEQSKNAIALQTKGQITNFQIIKNSMQQQQQEIIQQSIEQSQIIQTKKAVFEIFSENSQQKMMQNPTRRIGKLEHIPVMSEILGADTNSLSKNQDFQKLVENPFALMSQQFFKPQNAKIRFPPILDKFVRTQAAPISGIKYNSSVVSRIASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L493</accession>
    <name>A0A8S1L493_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0330066</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L493"/>
    <dbReference type="OMA" id="EAKYIIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000465">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TH1" evidence="8">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="213"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="7">
        <location>
            <begin position="469"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8062297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="60687" checksum="459FAD10EEE2D2E0" modified="2022-10-12" version="1">MHQQQIVLSYFGQLRLQISDVLNINNEKYFRKQASESELVLISRLTGKINKRNTEQQRTIVISSENIYNIDKKSIKRKISIHKIFGVTVSRSSYEFVLHVPQESDYRFKSQENRDIILYYLSIVLKLNNKDGLRIYIVDNEELDQFCLHDATSDCKKQIALHPKTKLQLLYPENYQLSYINLMFTQNKNVKNKNISILYVNDPLNLRIKLEDYQKLAIIAQGSFSKVLLINQKFEKQQQFLVLKTINSYETDVNILQFHLENYTQSVYLEKLELCLRQQDQIHFFFKFVKGGDLFTHLQEINYFSELEAKYIIAQIALGLSYLHESGTIYGDLKPENILIDENGYIQLTDFGFGRLRVYQEFKKVQSINFTVEYAAPEYLLQGDLTRMSDWYSLGILLYELLIGISPFYHPNHEIAIRMICKGELFFPKTYAISYECKDFITKLLQRDSQSRIGFENDFKDIQSHKWFYGLNWQELSEKKKLLPYIPKINDEDIVPQQYFRLEKLNDEEKSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5M1</accession>
    <name>A0A8S1L5M1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0260303</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NCEDAIG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1502" mass="175353" checksum="FF9C2BF6F3A40DCE" modified="2022-10-12" version="1">MISNTISINSSFKCNIYYHSPLQCIQSTSLVKCKYEIQENICLETEQRNLGCSSNLNEEACIFQLTDQWGNDVNCIFLQRCQTINQVQLQKFNCSDKLNKLACMNLQQKECVWVNKCLNINEDSKIKTSSECQIYNLPVTPYTCQQFQYGLCMSNGKDGDFRCVEVRLDQLQSISCNQQGLNEKACIAIKTLDQKCIFENKRCQSIESFQIISCDQKLNINACLFSKQLSRQKCEWFESSCRIFQEQNYKCITKKIVNSFVCQNSQGLCMYDEKQKSCQIIQQNKINQLHCNTIGLSREGCLQIKNENCSFYGGICQELNEQDLKSYLCNMDLNEKACINLETEFQYCQWNGIKCERIFINQDIDCPMNFVNKSIKVNGNVCQAISKPDILCKYNQKTNLCVSSLINDECNTSFINLLGCLSIQNNKQACQWIQNQCKQIKIDIIKTTTCENLGYANPYACSQVYQNDSIGCFYDKISQKCKSINVDLNHNSIKIFLNTITCKDQSLGINKVLCASITTELTPCRWHQQQCIYINQKGDIANVPCIHLQYANYQTCSLVQYNNEPCLFFENAKGCINQIDSQTNCETKGLNYIGCQLINKNCYFDKIMCIIKQDIDFIIENNDNDTKVNDYQIIQFNYNNNLKCNSRYLTKITCSQIITIGEKCIWSFNLNSCIEASIKSNDNCLAYSGTNIIINPNVCASIRMDIPEFCAFDAIKKNCKIQYYNCTTKCCTEYYMIGINAHSCSRYSSKEEGIYCYFHDSRCQELKADIVDISNQDLVKQYYNDLKLPCTSMNINSCHMINWSTSQLCYYNGQTCNNLNQQFFPNLKIFTEYPSKLNEFACLAIEATLSSQNQMKYFSYNSQTQRCKELILDSEYPFLNCEDAIGNKNLCTRYTGNKFCKWDPETLKCVTMTLDEIAEIQYCNSNLNIKTCVENKILNCYFSYENDKCVQASEDIDCDQFNQKGKVSYSVCKYINKSGQKCVYSEDLKICIKSEKEYEDVNCNCDISISSGNNRVCYNSTCGYCRWDSNNFICYQNETDTTELLCNDNLNKILCQLVKKEACIWNDIEYQCQVFTPKTSEQFEILNINASYPYNEKVCLLISEAGYYFDRELKKCIKLSDNTNQLRCDQFQMNQYACLYLTRGHYCFYDSNEQIPNNFCKFFEDEQSICNTSNLINIEVCMNLPVSCLFNGSTLQCESFQVQQTHTYWQLFDYSKNKKHHNKIACASVGSAQTEFLNENLCTQQEGKFQQCNFEKYGFWKEYTCQVYQFLFQNVDCGIQQNQQDLICQNMENQNDCPDQDLLEILPEYEKFDPEINISIEWSSINLTCRKQKINYYSEQSFIIEIKYFPKFMISITKLQKFYIISLGLKEFNFLIFQQKDSKSQNQMRKNEQQVIGLLETHFFEGYINPIRYLQRIYLQGKINQKIDTGAKKLCYSFKENRILVYYYQFQREYLKRLYYEQMYELTLDNRIYLMDQFVLRNPEIINKREQLQNQIELLKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9L7</accession>
    <name>A0A8S1L9L7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDT1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0340109</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GDT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9L7"/>
    <dbReference type="OMA" id="ILGHAIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015085">
        <property type="term" value="F:calcium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032468">
        <property type="term" value="P:Golgi calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032472">
        <property type="term" value="P:Golgi calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001727">
        <property type="entry name" value="GDT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049555">
        <property type="entry name" value="GDT1-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN HTP-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01169">
        <property type="entry name" value="GDT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01214">
        <property type="entry name" value="UPF0016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035968397" description="GDT1 family protein" evidence="6">
        <location>
            <begin position="18"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8063021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="254" mass="28082" checksum="2F2375FA4F4E1B34" modified="2022-10-12" version="1">MSLRAFILICLIFLGYCKESGEKHLTIFTQSIGSMLVSEIGDKTFFLAAILSMKFNRLAVFTGAAGALVLMTAISCAFGIIVPTLLPRFYTAIVVTIIFYFFGGKLLFDWYHMENEGDKEELKQVELELEELDKKLLSSHKILDTENPSEGQKTNLAVVVPIQQIIWQAFIMTFLGEWGDRSQITTISLAAVQDADIVFLGCSIGHIICTTIAVLGGKLLAHQISEKTVNLVGGIVFIIFGLMHTYDLMTGNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LAS9</accession>
    <name>A0A8S1LAS9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0340253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LAS9"/>
    <dbReference type="OMA" id="LACMQVE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="287" mass="33717" checksum="549A9722DE44D178" modified="2022-10-12" version="1">MQQQPQQNPTEEKKLNRIGKPKGMNTQKPLDKTNAWSSDQQSTQAIQALSRLGRFICFQQTGKKKIAIDQNDQNKATVDQNDQNKTIVDQSQQSKPKQKQPLDAPGIFLNSSQIQIVKLIGEYCQIVEKSNKNCIVFFFRSSHPEFEIINGVPQLINNFDKYGIYPALKKLAPMLNQKFLDEYFTYLQFQHGQLQYIKEIQVLYDDFHNKTLKSAETLLQCLFTKYLIKYFEDLDLLTKKNFQERVENIYEKFYNEHQTKQDYDACINELLACMQVEEEFDQQYTCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCR9</accession>
    <name>A0A8S1LCR9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0370220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCR9"/>
    <dbReference type="OMA" id="EYHINYW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="41"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8065337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="120" mass="14555" checksum="BBA0D5997F3B12B4" modified="2022-10-12" version="1">MAGVLKTVGDYFELDKYQNEIAPIVKENYDMLQKMIQTKEKECLNKNLDNEQKYIECMQKNAERSERALKRLEYGIMYWKQKTYECFHSEAYKDKEIKNFERCKPIANRELQEIFSSFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LH27</accession>
    <name>A0A8S1LH27_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0380094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LH27"/>
    <dbReference type="OMA" id="QCALINY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051219">
        <property type="entry name" value="Heterochromatin_chromo-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22812">
        <property type="entry name" value="CHROMOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="3">
        <location>
            <begin position="9"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8065801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="234" mass="27650" checksum="E108BE8605FC18E3" modified="2022-10-12" version="1">MNKIKRKLFSMEAILDSRTINDQLYFLVKWAGYPISQSTWEKADKVPADSNMISEYKMSVQMLGKTFYFDPLDQPPQIEDFVEVPPQKSELSTFNTKISEFQHQINTLKDDVRLILQSQLRLIKELKMSKNVIHHYDLQRSPGSQDKQQSIQSDMMQQYQQCALINYDGSFEQGDKVEKIGRAVQIKKNKNKMYYILWKRRNNGQIPKNSWVESEKIIENEPVKVCQYLWNKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LLK3</accession>
    <name>A0A8S1LLK3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0400122</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LLK3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="175" mass="21570" checksum="FAC23A04D713C355" modified="2022-10-12" version="1">MSIINKIRFLLRKQKVLRMKMMIQVENVHIQKVNQNNKQDIQIVQNSQIKKQKKHYKNYKFIKKNQNNYYIKENKIKDLNLIQFNLLKVQQMHNKKLIFLRDYVKLEKIYLLKKKLIKYHKFLIMKLKKNKAFHLIISIQLQLLQLQHQGLLLKKSCYDLFLMIYFKYIYATILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQ74</accession>
    <name>A0A8S1LQ74_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translocon Sec61/SecY plug domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0420208</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQ74"/>
    <dbReference type="OMA" id="PMMRQMF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3370.10:FF:000009">
        <property type="entry name" value="Pretranslocation protein, alpha subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006341">
        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="441"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="4">
        <location>
            <begin position="42"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8068535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="474" mass="52456" checksum="59265342A4C6FB2F" modified="2022-10-12" version="1">MANKTKILNYMRPAMAIIPDVAEPERRILFKYRALWTAIATLLYLICSQIPLYGIYKSSAGDPFYWMRVILASNRGTLMELGISPMVTASMIMQLLAGAKLIDVDQNVKEDKQLYSGAQKLLGILIAFGEAFAYVWSGMYGDLDKLGAGNAILIIIQLVFSAIVMIMIDELLSKGYGIGNSGTSLFIAINICENIMWKAFSPITHKTQLGLEYEGAIIALLHGLFIQSDKISALQSAILRDSLPNLTNLLATVLVFLIVIYFQGFKVDIPIKNNKVRGGLTSYPIKLFYTSNIPIILQTALVSNLYFLSQILYRNFKGNFLIRLLGYYQELENGQTVPIGGLVYYVSPPRSISEAIFDPIHTILYTAFILGTCALFSKTWIDVSGSSPKDVAKQLKEQDMQIVGYRDSSMKDVLKRYIPIAASFGGMCIGALTILADFLGAIGSGTGILLSVTIIYGYFETLKKEKEQGTLELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LRA0</accession>
    <name>A0A8S1LRA0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Large ribosomal subunit protein uL3m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0430012</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8068812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LRA0"/>
    <dbReference type="OMA" id="NSKYNVM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000004">
        <property type="entry name" value="50S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_B">
        <property type="entry name" value="Ribosomal_uL3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019927">
        <property type="entry name" value="Ribosomal_uL3_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03625">
        <property type="entry name" value="L3_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="25"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="276"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="393"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8068812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="409" mass="46771" checksum="EFE290B39424B527" modified="2022-10-12" version="1">MLLRFITTTHQLNWASLYYFTKHTRPKAQPHREKTRAKAISDDKSGKIAKSDQEQEQKQQQENLKKKIELEKLRALQPKMHTWSSFYETHIPQTKKIPKTIYLSLNEQIEGRRKHYKKFVQPPIDITEECNQKFEIKQHTYDDPFAQPKRAGLIAVKVGMTAQWDKWGYRHALTVLQLDNNQVVQVVKNDTYTGLQIGAGAVNIKTLKKPQIGHFLKANIPPKKYIKEFPITPENVLPVGYMLTARHFTPGQFIDIQGISTGKGFGGTVKRHNFKTQPATHGNSLCHRQLGSTGQRQDPGRVFKGKKMPGHLGNDKVTCEGIQIYRIDAVRQLIYIKGSVPGKPGSIVYLRDSWKVQKKNKDLLNFPTLAVENGKEYAKEIVMEAPLEDPELQETHENDFPKAGEDAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LS45</accession>
    <name>A0A8S1LS45_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphagen kinase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0410006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LS45"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022413">
        <property type="entry name" value="ATP-guanido_PTrfase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02807">
        <property type="entry name" value="ATP-gua_PtransN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="1">
        <location>
            <begin position="74"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="156" mass="18282" checksum="0F632C6AB25E9870" modified="2022-10-12" version="1">MIFNQYFILVLSEFCDVYHKQRKIFLFIKKNIQYYRNQIQCVFKLDAHKLQLDADGNVDFAKIQARWDDLKSVKVSLGNKYFTSEVVEKAKTLPQQDQQRFLNLMLAGLTNDDSSVGISATNLKIIILCLLLGIINQRLPLNIRRHKRRISLEYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LVP9</accession>
    <name>A0A8S1LVP9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0500036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LVP9"/>
    <dbReference type="OMA" id="KAHAFSF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="121" mass="14253" checksum="CD4B18B3D84F0355" modified="2022-10-12" version="1">MKDLVNQNRSHLTAEESLKKLYEYSYIQDAKQRSYHLRLQRAHAFTFGTIASIAVFYVGYNLVEGVWAKLSTTNQITNLFRIAQNPVRSVYRPEIYLRDQNADQILAQKTQEQHNPLKLWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYX1</accession>
    <name>A0A8S1LYX1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0460126</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYX1"/>
    <dbReference type="OMA" id="SSIKWEG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34726">
        <property type="entry name" value="GBP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34726:SF3">
        <property type="entry name" value="GUANYLATE-BINDING PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="517"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8070763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="631" mass="73433" checksum="BC64186D136AAD92" modified="2022-10-12" version="1">MSSLFKEVSLKQFIADKSQKIQILVTMLKDIQKEEEEIMKQFNKMTKEARSIEELYEIMEMINYEMSVRNSDKLIIMDEINQQEEYLLQLQASLESQVRNILKEFETSQKFQQTRSLRQTGRFLQFKDNNHNLNMENSNVQSNIITSYDFEEQSRLYDKIQDNIFNDPTKYNSQEGIDETQVYDWILDIDLINNVQFGWPVYISRPFQAKMELVGLGNNNNNESKSSIKWEGATVAVVGLYDKGKTFVLNNLTQSNLPSGKKVTTKGISFKHVDVDSGTQLILVDTAGSYSPVKIKSALSIVEKEATEHFIIDLVFELSDYFICVVNDFTSLDQRYLDRLSRQIQNSSKTFREIIVVHNLKEIETSEILQHVWTTQVTQIYSTGGSIQRTKVAANNPRNNELQAKHVLWFKTQYTRHVCLVSDDSQLGLDVNPWVFSLLKYWLKSVFVPVNRQFSVCESILQYSTSKLTQYFKREVNVKLIDTDNQLVKKIVQMEADLQNGQLKIPQTNIDQSGLILARPDSFAPATDIIANDKYTIYMDVPGISEEDIEMYRQNVVTIVKGNRKKPYQEEQSDHIKKQERKYGEFTLSFRIPENFERKWKHFGIENGVLKIVYEKDKDDIIPNQFMSQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4F1</accession>
    <name>A0A8S1M4F1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0510112</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ubiquitin conjugation factor E4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4F1"/>
    <dbReference type="OMA" id="LEECCKF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000055">
        <property type="entry name" value="Ubiquitin conjugation factor e4 a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019474">
        <property type="entry name" value="Ub_conjug_fac_E4_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045132">
        <property type="entry name" value="UBE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931:SF2">
        <property type="entry name" value="UBIQUITIN CONJUGATION FACTOR E4 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931">
        <property type="entry name" value="UBIQUITINATION FACTOR E4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10408">
        <property type="entry name" value="Ufd2P_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="11">
        <location>
            <begin position="857"/>
            <end position="931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8073532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="976" mass="116321" checksum="F9FD0F622E876615" modified="2022-10-12" version="1">MFADASEILFLEECCKFTVDPAKADKMIFLADTYKQYFQGRTNILISQNQDILDACILERLKYPQTNPYVNLVQIKYNIEYLKGRPWYNTPEIGNLIESFKIMVQNYLMTILQYPDQFSPQRCISDREKEQNQELQKLIARLFDIFDTTGFDSDIHSLFIQNIRENYDENMLHIQIIQYSRNCISKLHIHNDQVNLYFAVFLHYLKDNKTKELIMSDKIAKWNVKTDKGDILEKEYFLGPIFTISLLQENQYVRQRAHQNLTGKMTKRQFQILLKQYQQVGHNHVESLVTLIKTCLSKNTFQQTIQFLNHVCLCNKFKTKEGYQHNRQMRIKQNVSSDGFLLNLYDTLLYLSNKISSRSDDTYTKIDKQFWYTLPFENEQMLLFSQIPLSKQNLQIGNVSYLFFYTLKYVHIGVIPILQKLEDMLKEMQDNKELLELLAGQPEEARIKADIEAMDSEVHQLELVIFNYGRIKDTIQLFDTFIYLFKEWLNIDNMIDGQTLWQKPDILNYIPEFVINVIIDYIHFYMQHYEGFVDIFFDFQKFTFFAELGMYFIHLPIATNKYLAGKFIEMIFFYTRVTKNIANIPLIFISNSLIKDNLLLGLIKQYSAVAETGANNQFYAKFQYRLYINEILFQLMQIDIYQNQIKKYIECESGQRLIKHMISDMNYGFEEIWTTYLETYQKKLKDAPNSLQEKYNKKRELDLIKSQIKSNLQNMKSNLKLLVEFSFHIPKVLMNEVFQEMILKMINYYLDNLLNERLKEKLDSLKKIAEKEFRLETFLTLIGTFFANVYNQKKVVSILVKDDRSYNIENFLKLEYFFRQKIAGQQEKVERLSKFIETLQLKSEKKKFMESILEQTQIPERFQDPINGELMRDPVLLPQSKEIMDRKVIVTALLEKKQDPFTATPLDTKDLIPQPELKKEIEIWLTEIKRQRDLKVQEAQKNKIQTEIQFQQSQSFNLQDDEDDLKVYKGNKYEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8K3</accession>
    <name>A0A8S1M8K3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0520110</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8K3"/>
    <dbReference type="OMA" id="DHMMESK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000234">
        <property type="entry name" value="Putative copper-transporting ATPase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="530"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="901"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="931"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="4">
        <location>
            <begin position="6"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8074163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1007" mass="114348" checksum="80F4A4E41B12A2B2" modified="2022-10-12" version="1">MESNIVVYDLTVSKIWCTMCANKIKSSFEGQPGIQYVQVNVMAERVIISFDQNIITMNQIKEIFEKNNFFIVGQPRLINNDSNQQRTCQFFFPNAEYDLEELVDIKNNLYKEYQGGLSGIQEQSFKYNKQSGQLMSINYQPLSISGHQLKQFIESFVKDKIIKNDEKENLDADLSLYIYNQLLEKFRKKERLVESVTKKKLILAIILSALFLVLCSIMPHFEFYSPILTYPNEKSIFSLYLVLIFIMTTLTLVKFGTQTYKNAYQLFIKYKMFNMDTLLTLGSLAAYVMSIFMLIVYAIESENESTEKYDQLERIMNVIHDLESAGLILTVITIGKYFEGKAKQTIMDMQNQIFPQEQLLKTPIVTRVQVKNQDYDIDTQHQCEVSLLEKGDLIILQKNMKLLVDGVIVKGSVTVVDSICYGWDDKFEATIGMRLKSGADILDGSCIFQVEQVIEGSMLFQIAEQLNLAQSEREDKTFGISAMLQSVSQKFVLGVICVALLVLFVWILLIGLDIVEIDEYCVWCFPFERAISILVASCPCALGLAVPSVVIITLNLALKCGILVKKNTIFEQISKVKCVIFDKTGTLFTKVDHIDSFTLLSKNQTEVCSKNQDKTIMKVKQFQRSLVMSDPIETKREQQYCEGTLQSKLKYNQTLSVQDLWAIISILEKEFTHPIATLLFKESISRQIGRQSTFVLTDQPKLEKNGIIGNVTRTTDKTQFKCLIGNLKHLENNNAILEDYLLNKCRFLEGRGKTVIIMAIDNIPEVVVCLDNKTNLRPEAKAVIQYLRQKLKKQVYILSGDSKKTVNSVGKYLGIPSINQFAEIDAEGKQKILKELRENNQEVMMIGDGLNDILSLQQACVGVAINAKSELNLMASDVIMLNENLWKVASLFNLMNIALKVVYINLLWAFAYNIFMAPIAAGVFYGQGFTISPMISSTAMSLSSVIVVLISNCMRFIKFDPSQQSSLRKVQDEKISEQSQCSYDSYIEVNSSSQSNRKTNAEKIITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MB98</accession>
    <name>A0A8S1MB98_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0580243</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MB98"/>
    <dbReference type="OMA" id="INADWIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00141">
        <property type="entry name" value="NT_POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.210.10:FF:000002">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000020">
        <property type="entry name" value="DNA polymerase lambda"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.110:FF:000005">
        <property type="entry name" value="DNA polymerase POL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002054">
        <property type="entry name" value="DNA-dir_DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028207">
        <property type="entry name" value="DNA_pol_B_palm_palm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018944">
        <property type="entry name" value="DNA_pol_lambd_fingers_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022312">
        <property type="entry name" value="DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029398">
        <property type="entry name" value="PolB_thumb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276:SF28">
        <property type="entry name" value="DNA POLYMERASE LAMBDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276">
        <property type="entry name" value="DNA POLYMERASE TYPE-X FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14792">
        <property type="entry name" value="DNA_pol_B_palm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14791">
        <property type="entry name" value="DNA_pol_B_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10391">
        <property type="entry name" value="DNA_pol_lambd_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00483">
        <property type="entry name" value="POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="4">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; Schiff-base intermediate with DNA; for 5'-dRP lyase activity" evidence="3">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR622312-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8077698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="616" mass="71975" checksum="7BEE14E56A820E6F" modified="2022-10-12" version="1">MFKGISFMFPLPTVNLTNNRIKTLRNLIELNGGIIDLNPRTIMIVGSDATLESCQKQLQKMNLDFEQYRIQFINADWISQSLQAKNLLDFKKFQLFLEIEQQQKRVSPQATDTKFVYVESLARTVPIKEDEENQLDMESGEYTIIKPELKEKYEKKKQEFKRQMIKEHRFLLDYEYDKDLDNYHHQVDDGYEFLLDNFQILKKEEFDIPQQNKFYGDEDCQITEVRKPQIDFFQGLDMGKAIINLDQPLVNAQIKETKQFNPGSKQQIQFWENKKEFFICDAGSAQKCFNNQIIEELEKLLKIYTNEKDKGRCIAYRKAIGYIKSLTFPIRSREDLKEMPTIGEKIKKKIIEIIQTGQLLKVQKLQGQDKNVAITQLTRVWGIGPTTAATFYFKGIKTLDDLRKNQHLLNKNQQVCLQLVEELEQRIPRDEATIIYEIVKREIDDLSGVPGLYKATACGSYRREKESCGDMDILITRCDGKSSDGFLMNLIQRLEGKLLTHHLTLPKKGEHDSESYMGIGRINNHAIHRRIDLKLYPKEQYGCAVLYFTGSDQYNRSMRLWAQKIGYSLSDHGLYPTQRGSHNKKLWKGEVIACEEEIDVYRILGLQYKPPKERSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEP4</accession>
    <name>A0A8S1MEP4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Adenylate kinase active site lid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0570088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEP4"/>
    <dbReference type="OMA" id="IKVENTM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="2">
        <location>
            <begin position="145"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8076851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="245" mass="27560" checksum="A7CAF3CC094E4712" modified="2022-10-12" version="1">MKNLIIRYAASVAKPGQQFLVLFGAPGVGKGTFAKLLIKDTKFNHISTGDEIRKILKGNTSQSFDQKLIVTIKDIVAKGGLVSDEIVMNILQEKLKEPESAKGVILDGFPRTLRQLDLYEKILPTNVVVNVTLRADILLEKLAARRTCVGCGTAFNICDINRDGYVMEPLNPKKEGICDACGGKLVVRDDDKKEVIEQRMKEYEEKTHPLLDRYRAMGVVIDFEVKKGKKDYPRLWDLVKQKLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKG3</accession>
    <name>A0A8S1MKG3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0580037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKG3"/>
    <dbReference type="OMA" id="RECQSIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="308" mass="36879" checksum="20727E0AA8519F0A" modified="2022-10-12" version="1">MNENQHKYIQIFRTNESKNDNSIIKQLRKSIVKKQLPLLTDAQNTQPSSNIQIATQRSEMQRSGKIFVQKKNKLESNRIQRERECQSIYQTIVNQKVTQKYTLIELFKNFIENFTQIINIKNEKYALNIILKLIQSIPKIQDVLDIHKSKFQGITADQMIKQLIHHWLDQSIFQDYRKFKLDPIYITSVDEINKKFEETLLNLYDEQINQFSNRMINQIFSLKEKQFYIEGQVKLSNRGNQQLYQKTLQLIDRKPNYRALTSDVVERLQIQEEKLGKFDLIQNKERRKQMSLNLFVQKIRANSKYIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKH9</accession>
    <name>A0A8S1MKH9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="9">3.2.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0590069</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="9"/>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKH9"/>
    <dbReference type="OMA" id="LNEPALY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000113">
        <property type="entry name" value="Glucan 1,3-beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF34">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035736638" description="glucan 1,3-beta-glucosidase" evidence="10">
        <location>
            <begin position="16"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="11">
        <location>
            <begin position="85"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8077913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="376" mass="42903" checksum="56F657204694DC67" modified="2022-10-12" version="1">MKIIFLCLMMTLVFSHIQPDIRSGKVKSKGVNLGGWLVVEHWMTSSSVIWEGVPEYYHGYGEYGLMSYLGHNVGDERFEKHRSQWITEQDIAELASYGINTVRVSIGFWIAGFDKTGGSDWKVFAPNGLKYLDQLIKNWAVKHNVAVLVQIHAAKGSQNGLDHSAPPVPGQSYWALYPENVRNTVDLAVFLAERYKQEIAFLGVGLLNEPSGTTDEATLKQYYLTAISEIRATGNDCILTVAPLLYEQDPDHFNDFALKETHVWQEWHKYLIWGYEDMNEDQILNIGIPGVQHQLDIWKGNPIFIGEWSIATTDNAPFASEESFKSFGNKYRDTITSAKGGWTYWTWKTSYDETQDISQRNAWSLRQLLRNGWFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKK1</accession>
    <name>A0A8S1MKK1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0650175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKK1"/>
    <dbReference type="OMA" id="ECTANNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035846138" evidence="1">
        <location>
            <begin position="17"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="12116" checksum="8D9D54F66B727172" modified="2022-10-12" version="1">MKQILAIALLILCITARKHHKQEGEEPTNPMVQCIQDNCMNEAFGCVFDDACAETMKTCDEEHGEDMKIDTLVECTANNEFASAFATCMQTNCASLEQVMRFFNRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKL3</accession>
    <name>A0A8S1MKL3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Photosystem I assembly protein Ycf3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0640230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKL3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="29"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="63"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8080918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="13247" checksum="CB5C0FDEF3EFC2D5" modified="2022-10-12" version="1">MIINRHLNGIKKHLISIHNMFIHQMEKVNLIQLIKGECLRGMNDYQQALEWYQKALDINPQYVYSLNGKGECLRGMNDYQQALEWYQKALDINPQYVYSLNGKGKFNSINQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MKU0</accession>
    <name>A0A8S1MKU0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0620178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MKU0"/>
    <dbReference type="OMA" id="FNSWDYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="233" mass="27218" checksum="C034C334686D6B38" modified="2022-10-12" version="1">MAQISFIKVFFTFPIIFYQNCIEPQGADCYTHLWNYNNKVVNCQGKWFMGQFGGSYEKVTLLLNYPQGRTMRFNFTMGKFNSWDYEPFYVIIDDVQVDSFTYGPFEGTPTSNLGFIILDQMVRPALRLFYKVIQIKVLMMNHGDLEMFALKFQILVWISLANVIIKVQNFSICSGNQTLLSKNVPFFIKSISLYDGMIVKFKDSKYFGGELQIYTQSQNCLPNYNFPKYKKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MLR6</accession>
    <name>A0A8S1MLR6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0650080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MLR6"/>
    <dbReference type="OMA" id="IKMKTEP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8081160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="290" mass="33598" checksum="3E36326054896E48" modified="2022-10-12" version="1">MRQQQPYFIRPPNLSSRHSTNKISFLNKEDTPSNSQGVRRKTPIIAQPLHSPTHFKDLHRINPIKRIPTLNSPLHDGSISKYKIINDLSVYKLPTTRRIKSDDQTTNIIPVKKQQSQHINNIKLRTDRRKNFTPLIKMKTEPNEIATHSISKQDESQDILFRLHKQSIPLEPKDKFASILGDKIVSSIKKIDKKTENFIELEQDSQFFLETKENKKNKEIPPLSQQTKERILDLLCLSTQDLKKKFAEQSRVGNSKINTRQPNKLVIPKTGKSRFPLDFFNALLPNSILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MMC4</accession>
    <name>A0A8S1MMC4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL15/eL18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0660095</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MMC4"/>
    <dbReference type="OMA" id="WGRVGQH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="6">
        <location>
            <begin position="72"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8081638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="147" mass="16700" checksum="00F5FF0A0B5C95CB" modified="2022-10-12" version="1">MVSRLRKCRKMRGHVCHGYGRVGKHRKHPSGRGVSGGLTHHRINFNKYHPGYFQKTGMRHFHLKKNQLHKPVVNIDKLWSLVTDETREKYSKSKDKAAVIDVTKAGYFKVLGKGRLPNQPVVVKAKFFSKTAERRIKAVGGACILVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MME9</accession>
    <name>A0A8S1MME9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0590097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000060">
        <property type="protein sequence ID" value="CAD8077965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MME9"/>
    <dbReference type="OMA" id="REEHNSI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8077965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="554" mass="66521" checksum="FE12664C30168918" modified="2022-10-12" version="1">MEKQLCLQHMQIIQSVCLERECYKQYYCCCECLLETHQNHQLMNANDFQDLEKSIKDSSQQLLQDIEFTQQIVFEQLNSYRTLVDNYISKQNQINKKEFSIIQQSISQRLLKIRHLILKKVLNEFQIKLLWTEDEIQEISQYLQDFENKDFIQKLADWQEKLREEHNSILSSIQIDIETEQCEITTLEQQDQSIYYGQNINGMLHGRGIIIGQTFIQDGLFKNGQFVYGLWLVENQAKEIISKEGVVDEQIQRQMKSIIVQTNYLKTIKDYDELKTSNIKRFQWDEQEQEKISQILQNVMNPKVVNQHRQLLLYFEIPEEYHYLQFIKYQDIKKINKDQLYFGSLMDDNQTLHGRGILFEGNYSIKDGIWEEGEFIWGIWVSLLNNQLFQYYGTITQEQLKQYEEFKLLQKEYEKQEQDEIQKLNHQKKLRFFKRIYQFMDTLISGYVHNVKLKELYNKNKLDKEIDVNEKYLQFENEPDIIRNNHQLYIGFQSAEGNPNSIGFYCDQNLIAGGYWENGILLWGTYHKYISEIDQINVQQNIPKEIQEKIQIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQ97</accession>
    <name>A0A8S1MQ97_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0640078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQ97"/>
    <dbReference type="OMA" id="NTREFRQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8080651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="141" mass="16751" checksum="FB8EF922FEB206FD" modified="2022-10-12" version="1">MDLEKYLAQFPNSNTNLNKFIQKDSLNLQCTYVPPIAILHKQQQKIDFSDVMNLLQNYQNYNTREFRQSHIDFDEKTFYVTIHDEKKSILKDGDDNAIIIINSQNIITVGIVDQFSKCKKQFLQTLYLFDKLKNDNYKQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQV3</accession>
    <name>A0A8S1MQV3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hyaluronan/mRNA-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0670020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQV3"/>
    <dbReference type="OMA" id="MIRISHH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039764">
        <property type="entry name" value="HABP4/SERBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006861">
        <property type="entry name" value="HABP4_PAIRBP1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12299:SF17">
        <property type="entry name" value="AT19571P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12299">
        <property type="entry name" value="HYALURONIC ACID-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04774">
        <property type="entry name" value="HABP4_PAI-RBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01233">
        <property type="entry name" value="HABP4_PAI-RBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hyaluronan/mRNA-binding protein" evidence="2">
        <location>
            <begin position="69"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8082019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="272" mass="31710" checksum="9BBD874002BC6471" modified="2022-10-12" version="1">MSTNMFNVLNISDDEDSKIQQAQQKTQQQQAKKNKQKRDQEQVIPVEQVKKESTQHHNPVQKQKGLTAEPHPKDRHSGTGIGKELRKEGGGRRNWGNYKDDLKQEKYEGAIEGKEVSKEQNTEQQGEEQQPQENVPQAPAEKTLADYYQARGVNVEEVLKKQQQKVQPVVKIDEEALKKEKLQVMRTREDQKREQEQKATKKQSGQQQAYRSEMSTEGQQYFGFTNDQRRNDRNDRNDKNDRKDNNNYNKGQKKQQQPNNGVQFDDKDFPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MRT5</accession>
    <name>A0A8S1MRT5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0670053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MRT5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="60" mass="7126" checksum="D9EE466BE9BBEC67" modified="2022-10-12" version="1">MDKQKVKQMADDSDNIQEIQLENRHRLEKQEILILKKLKLPKPPLLISTSLPKLLFFKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTM4</accession>
    <name>A0A8S1MTM4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0690094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="IMETKQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010441">
        <property type="entry name" value="CH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054517">
        <property type="entry name" value="SPEF2_D5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052634">
        <property type="entry name" value="Sperm_flagellar-bone_growth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14919">
        <property type="entry name" value="KPL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14919:SF0">
        <property type="entry name" value="SPERM FLAGELLAR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06294">
        <property type="entry name" value="CH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22946">
        <property type="entry name" value="SPEF2_D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1129"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="866"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1138"/>
            <end position="1151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8083069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1442" mass="172680" checksum="CC496365D6E6161A" modified="2022-10-12" version="1">MSQLLMVWLNDEVQLSKKVTSFEKDFNNGYLFGELLSKFNQQLNFEEFSNKDIREAKMKNFTLLEPTFKTLHIPFNFQIADQVIKGKKGVAMQLLYQLQMQLQKVNDPHDVMMHAKTGKYNVIQPLMVIRQPKEQFDKMEQEFFVSKLNEKNKAQKDVNLDKHLGKFTSFAIQQAEKERRLKIKEETEERNLKEEMRKIQLNKLQRNMAFMEDWNQKGIENWKKNQQIRHKNKVADEKFKSTIQKVTEERLVQTQQMMRSEMEDQIEQFDNRTTQEPVSLGLRNKMMSEFLRKERDKRRRKMIVDQEKQTDELQRREYTVLEKLKQQSKQEKEIMYEIWRAYQCKEVIFENRQLRDENYRNKQELNVINQKFKEEEMLRALQQEFNEEIELQQKRQLEINVEYKLKIRQTNYEKCKQLVEILFEIEEQLYEHLQNNDSNEFNKQFTRENHHLFKQGMELIPYKRFRTYDPIKEMKKQESLNYKQKMFLAKMEMQDYLDGKGAWDIYQAQNNYTLGNLVRYLIESQFQNNTLEIQEQTDIPYVPFRGSLIGFSFSGKKTISELLAKKYGIQLLSIDVIINELLEYIKKYEQGEEHSQIWDQQQIELGQQISDYLCQGLEIPEELYIKAIVQKIKRLFDKQDFEQIYEEYKAKYQSFNPRQNRTIDEFNDDGINWEDFHRNNMINNKPYCKGWLLVGFPHNYDQAKLLEKYLTGIEPQDELTPLQARLQEAARVVKPIDYQIIPRKKQESGVGVCVYLEMPSNEQCLRRAIGRRYDNHNHSLYHLDSNTPPVDNAPLIERIKPLYEVDNLQQLISDKNAYFMTQIQGIQNWYSNFESADNSNNLNAFISVDAQNNYERVFYDVDKIFQNFLQIQINKIQQKYDKQQNDILEQEELIKQQQEQAELDKKCQFKQVQLDTIPQKQPSQQFLQYLHSFWNQIETKYISLQNIFKGFREQREFITNYLMDIQRKFIDLINTPDEKFYHVRNFQEQYNQFVQENPDLCEEDVCKEELHQRVDDLYDQLIDIIDQKRDDLQVEKQQIQNSQFIENEIELFLLQVRQLLAIELDRAQNSIQLLNDYYGTLEAIELQDIQPSVGELQLQDGDDPNLRLDKLIADTLRIFAGEEEIIEDKTKKGGKQPAKKDEKKGKKGKEEEIVKKELQNPDAINTINLEKQIFKDRVDAIKRYASQQIRTYRQAGDQLYLKIDEWIYYTHQTEIKALDAISGLFRGYIERVERIQKELQLQFVDVIISHEVLNFLTPIPPPLTAREPLSKERYSISQLYYLIEDLRAITSTHLLIDIKQLALLLARSSYGVLKYSPSQWMQALQILDQNGYVNVRQICTYLILLSSPTPKEDELQQYSSKLGQSLVNKDTFVNTPAWFDEYERISEEENTNYFDRVIYIKELLFFVHKDENEMLATKQYIDILNVEGDRFVDYLLQNMQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MX73</accession>
    <name>A0A8S1MX73_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0730015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8084840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MX73"/>
    <dbReference type="OMA" id="PIREQPL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="331"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="408"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="515"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="659"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="719" mass="85130" checksum="B70C508CEC781229" modified="2022-10-12" version="1">MLQLQLEVRTIQFYINSMTEFCSIQFIHNLSSTPLKSILKCRCRDIQEIRERIDLNIQYFEKGNLRLQIYDGQIIFGEATINLGFYIENSLPVIIDNITIQSKYDQEAYVEMSLGWNQLEQIQTKEIQQINPIREQPLFIKLSQQTANRVPIEIYHKIENRTMNKICSEQNYFIENKQEQIDNKSIEQKKQLEDEKIEENPKANLETVNSQRFQTPKDNKFAKKQKIQKSSGLLEHYKKQINTRQTYSDSKTVVKEQRLRILQKSPNDQGVKIRIHSHQNEEYDVNKGITKQKVICKKAIITEDYGKDGQKNLEPGFFTSRQLEIDQQIEKECQQISIQQLLNENQQMKNQIQKLSQENQELKDQLIKSEATFNLLSETYTTLRNEYKKVQIKNYDSSNNTFIMNKEYEFIKKELEQKQKEIEFSKKETELQKIELEITKRENIQLQIDLEQIKKEVINKNIENSNLLKDIQDKQYKINTLEQDLFHQQNNQTISQDINLQNHLNEQNEYNNHNLDQMKNIIKNQKIEIDNYQQELKQTKQNLLFQEKGGKQQFKCQEELQQINLKLELEINQLKQKLIESNQEIKSKALEIEKLKTQIDLYLQSPDKQNFDLTYLINEYSTLNASLKQENQTLVHQNRKDQVKLESLQKQLGINKNIVELSEQRQQSLEKEILQLEKAVLNGKQNMADVINAVLECGGPDLAEAVERFLVIRRSMKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXC3</accession>
    <name>A0A8S1MXC3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0730099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8085008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXC3"/>
    <dbReference type="OMA" id="SSIRTYH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="234" mass="27144" checksum="4F86664DE5ED82BB" modified="2022-10-12" version="1">MSVYSLNISGLKHITDSVYCNHQQQESNKTTKGHHKINSSQQLNQKVLVNSNEQIVNQSHHKLSLKKTIYSYIDTISDPKKNIHSSRCPQNYETMMHRNDFHLKKYYPRGESIEKEWKSSIRTYHTEITQKSVSPPMNQKPKIQQQSRNPILSPQSGKKQRPTKKTQFEKVPKKENKNGIKIFKQANSSQGFVNTLDQNSPSNKPITIISPRFGEGVQMKQIFSEFFVNQQTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXN8</accession>
    <name>A0A8S1MXN8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0620168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXN8"/>
    <dbReference type="OMA" id="HMPWFFV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46277:SF3">
        <property type="entry name" value="BINDING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46277">
        <property type="entry name" value="OS03G0850700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="84"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="264" mass="31428" checksum="C5ED71C70CEF66DC" modified="2022-10-12" version="1">MNQPKNNIILTDDQHEKIEQLLLKVQAEVPNLVRENLIQKYQNKDHLIRLMIAREWKVNDAFEQWKRWVEWRKQYRADDIKIEEIQQEIDLRKAFWNGVDKFGNPCLIIKTKRHFPGQSNPETLIRFFLYIIDQGIQKADLAGTGRISVIWDREGVTSKNFDTSMFTIMKKVVTLVQDNYAERLNQLFILYPNFLVKSIMTVIKPFLSEKTKSKIILCNQMKDLQQYVGENYQISDNLIYENVEQVQGQYNEENQNQIEEIINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MZN8</accession>
    <name>A0A8S1MZN8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0680013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MZN8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="164" mass="19418" checksum="A5787006F83DA9E6" modified="2022-10-12" version="1">MESFVQNNQYNQKQFCLQCNIISQLLKQSKYTNNRKQTITFCFIKSNIKILQEKQLIPIINFQGRENCLVFESTFSIREFRLFLIRQIVKKKLYTQIEFSPVTNLIIKTFKGIEGLKFLRAYQLDILRIGYKNAKFIIQIYSLVLDLNLSNHLKISIIIELGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N1D9</accession>
    <name>A0A8S1N1D9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0760142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N1D9"/>
    <dbReference type="OMA" id="YDMKLNL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8086370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="200" mass="23565" checksum="0CA11D3549B914BB" modified="2022-10-12" version="1">MGNKCIKNKENQFLILKGGYPLMTPQKQHLLEQFTYDMKLNLDSFEDEFFQKEYIINSPVQDDVLKTKQENSIDFVLESPTFRDQNLKNYQVQRNNSAPVQKTKKRPYVSMNKSKYIVYEIPEHSNQKKIKPTSRSLSKNKQLRSIQKVEGHKPRKLNTNISNNVIKQLVSQSKTQQCIDGYLKKKINSWKFNDSVKILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N3V6</accession>
    <name>A0A8S1N3V6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0770033</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERGIC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N3V6"/>
    <dbReference type="OMA" id="YDLSPFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF25">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="8">
        <location>
            <begin position="3"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="7">
        <location>
            <begin position="132"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8086662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="321" mass="37139" checksum="B3FB4F9DB59AD26C" modified="2022-10-12" version="1">MGLRQLDFFRKLNTDIGDTSSSFGGFLTILAFALVTILTLNECRLFFITELNYQTVIDNDSEQFIKVHLDIIIGAPCMVLSLDQQDEVGVHVMDVSGTLKKISLDKDRHVLPSIDNNERPNYIGSEQELIDAIKAINQGDQCQLKGFFQVNKVPGNFHISYHAHHYLIQRIHQRELQTFRKLKLDHTIYDLRFGEISSAKLKKYSKSLQKFQLPWEQIEKSVPEGEKQDYEYYINALPVKFYDVNELNYQTLYKYSINEAQMPRTFTEIDSIYFKYQISPVNMVYSIQKKSVYHFIVQLLAIVGGVFAVIGILNSIIQKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N423</accession>
    <name>A0A8S1N423_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0800080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N423"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="177"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="281"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="504" mass="59576" checksum="C285B0588A618319" modified="2022-10-12" version="1">MKLNLTPQTKVSRMDDTFSTDIHNMCYSRVNILTNEVEKLTDVLGTTSQEMEILKEHNLDLQNQLTEMEQSNQIINQEFDNICERLKQKSIEVEEYAFQVQKLTLQKQELTQNLNKVLNELEQINQVIDIKDQEIQSGKQQFIAKTEELQQSLQQTYHLDQALKVVKEEKLQYIYEYQQLQKLVEEQQQQIKELNEFRITYETLKVDLNILRVNYTNLLNQKLEEKKELEAQIDVLRDMHSYTIEDAQLKYADQIKQEIESFKQLYDEQYKFERMTYESTLDQLKNQIGTLSMQLQNAEILINTHKQNLMNQTQLSLFEKSNQVSELKAQNYVLQESLIKANRELNELKQQMENGNIIRFDEDVRKQIEYKLDLCQKLQKDKEELNKENEQLKRENRLLKENNQLMQQKMSQQQSNISQLPQSVQISQISPSGIQYGQKINDSEKIKYLLKAVDQLQNAIKDRDQEITKLSQMASTNVGLSSLLNKPNSRVGYYNLNSEDINSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N545</accession>
    <name>A0A8S1N545_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0670117</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8082164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N545"/>
    <dbReference type="OMA" id="LQTHYHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08291">
        <property type="entry name" value="ETR_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF18">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="17"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8082164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="332" mass="36701" checksum="357BFAB0674CC0AF" modified="2022-10-12" version="1">MAELPKSYLGAQVIESGKPLQINEIQIPELQQGEVLIKVEAAPIDQLDLVSAGAENYLNKQVPFVLGAEGSGIIVAKHESVQNLEIGQKVSFLTLSKFGAYGQYSVANISFVLPLPENLSFEQGASAIGNPVTVMLMLEEAKEQKAKAVINTAAASALGRQFVRYFQSNGIEVINIIRRKEQENTLKQDGAKYILNSSDPNFLKDLEQLITELKVTLFFDAIGGEITGKIFKLLPSGSITYIYGMLSGTNFEIDGKEILTRGKIIKNFSLLSTKHPFNPFSHQEALSRLHELLQSQLQTHYHKQYSLNQINEALEDQKQNGSIGKVLIRPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5H9</accession>
    <name>A0A8S1N5H9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0790058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000082">
        <property type="protein sequence ID" value="CAD8087680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5H9"/>
    <dbReference type="OMA" id="LYIYWIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="161" mass="19286" checksum="14246692EC871124" modified="2022-10-12" version="1">MTTFGSLTYYCLKANGVKSKLTFGFLYIYWINHFFTVGSFIGVTFSLPWAMRHLQQVDQKSNLYQQLTQIQNLIRNRKDESDSELNNMFYALRVINNEKVQDQKRTQLVENDFFDKHPEYVPQVMQENLRLPYLDYFLVRFAYFTFTRWVTRVGQFAGIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N8V7</accession>
    <name>A0A8S1N8V7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0810115</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N8V7"/>
    <dbReference type="OMA" id="QEQFCKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01765">
        <property type="entry name" value="FERM_F0_F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF159">
        <property type="entry name" value="LD03170P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="1">
        <location>
            <begin position="127"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="2">
        <location>
            <begin position="491"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="837" mass="100890" checksum="7F7D0E9AD5081BD7" modified="2022-10-12" version="1">MQQITNQKFESLREQLTSSYKQSQIFSTSKYQNQLYSSSIIISESSFLEQSQLAQDQEQEQGTIPSSVKAMKKKKQYARVMDSQYNLKELLQSNNKSIIPKIIIIQRNYRRYLYMKLFHKKLRQLRNRKFVVVEFHQVEKNYNEDLTIIVDRLMSDIKCNSILTQDELLSLFLNIEQIQKLNCKFYLTIQQILQKYKHFIIISQQFKIYIPFFKIYYEYCSRFQLEYINKLRSSKAEFNLYIQNIETSGLLKGLTFESFLVKPVQQLPRYVLMIEKLLKYTELNHPDYNGLRQIQEQFCKINEQINQMMNTTQSNLKLLAQFGTIDQQEIEMIQKKQQEEEQFLKKLQQQVSIQIVSTFKQKDGIHTFIVYIIQIQINNYRIRTYQRYSDIIELQNILLDRNFRIDQFQTNCVNLISEDNLIKIRQESILNFLKSIFLNKQMKKYEDILIQHLDLPENFFILDQDRNEISVTMRGSYTTQMRNTLFQEDEFEIIVILPDDKSITLQITKNCKTIDLIDTVCRSIFLDRNFDFKLMILQNNNCRYLDDNEVVYKVMKNYNHKKKNIMSKLINEIYYSVSSQSQPKFYLKKYLYLCYENEFQEYNKNKRRSLLLFYQELSQLKFNNSHLNQQDYVIQCALAICILYNNEIHKIKTYEQVRMQWFQNFVPLLILNSVNKIDWKKQIFEQIKKILIELQELQNQQECRNEIRILEIQFLNNLYKQKLHGIKIFRVKMLYNSFLNKQKRMPIKVSLGILHNEILIFELNQQIPLMQLSIQQLQSISIQNSVIVITFLEGIITFQHQQIIEVYRLMEEYQQINLLKEKLGYPETNDVSQCKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAE1</accession>
    <name>A0A8S1NAE1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0800146</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NAE1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000275">
        <property type="entry name" value="SRSF protein kinase 2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF9">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="60"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="269"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="590"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="590"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8088219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="650" mass="76683" checksum="3BC409FE6A650AE3" modified="2022-10-12" version="1">MSQEEQQEESNKYGKAINEQIGELMEHLDREHDSEDEGMEDYKIGGYHPVHIGEILLNRYVIIQKLGWGHFSTVWLAKDFKYDTYVALKIQKSASHYLEAAYDEVEILQKVAQNVQNPVWIQSLKEYYADQGRTSFNRDDTHTVQLLNSFVYKGPYGHHFCMVFEILGVNLLEIIKRYDYKGCPMDIARRMAKQILIGLDYLHRICGVIHTDLKPENVLLCLSDEEIKDIVENGQLTSNQLFSDRIHIYRKMLGIDKINVQEPIVKKEDDELSENTTNLSKTQKRKLLRKKKQKQQHEEQRKESQEVQVDQIEMPKSIKELFQQQKKISFTQQKKLPDNFRLKIADLGNACWIHHHFSTLIQTRQYRSPEVLLGIKYNPTADIWSFACMIFEMLTGDYLFEPRQGPNFSKNEDHLAQIQELLGKFPYEYSTRGVKAKRYFLQNGQMKRIPQLHFWNLHNVLTEKYRFKQEEALSFASFMMPMLHQLPEYRTTAQELLKRELCQNNLDPQQRRCLLKKYIDQIFQFQNFRDLIPSSGWLQQKTDTRVGHKMNEDEYKKYTKQKRVNEEAEQLDQAVDGIQPTLLSNYRRASTRSYNGSNSQTPQQDKFKKQDGKYVDHRVIDRSFTDLGYIGYGDGIDLEQLDSTGNWQFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NE18</accession>
    <name>A0A8S1NE18_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0780158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NE18"/>
    <dbReference type="OMA" id="IDVNHYF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="891" mass="104389" checksum="751E2E52FCE78747" modified="2022-10-12" version="1">MIDEISLFASFIQLKTETRYQRSKFSGLCLYPSIVLSIAFDRSTVNIGTVLITLILTAILKSHNKLIYFLRVLLLFPELLLQNQNHALISSFLINQIYVPYWPSQIVFQMILVRSVNGLYLGFGLLMLSRYFEILKRRFFISFYIQEYDQHQKEYVLEKLPIDIFLINSEANILYSNHNVKQFDNELQRKLSNAIRKKKTNEITINNKYYKLEQYHSDYILTQSQFSCGDVELFIKLSQQIDQLYNLICQDYQKWNNLRAFKVVKESDLSILGQCTTDCRLLKQQIDTINILNHDCQQIKYQSSQKDEFFLKNYVVNVIESMVNRLQEHFNLIQLEFEEGIPEKLVGNKHGILFSLYAFLMSAMLVEQKSDNLTINVRIAQVYLDKSEYDLEISICFPCSNKQMKLYCQDEFENENEKFLLIMINQCKQLLSIDYKTDGDNIIILLITNVQLCKKSDKSCDNIQMVDLTFTKNVIDVNHYFWNAKRFASPTMTYDKSLRSPLQKCTAKFNYAADSLASTIKLQSIPNSAQASAANSNAQSLENTLIKNEDIIKEVENAFKYTIQFPQIQDDIMKLLEQTLQAAYNEGILDHDKTILDIGYNHIHYDKYSQISVRSDTPFVQDYGDQLIDQNDNEFQKSKSKQFKTPQLKPRDLIRIDNLGQQLIHEQNKKQEQVNRSIQGKRNKDQKQLNVLFSPSVMSKFSNNLVSKVQKSKTQLPNIDDNNDIKFHIRVRDHRLRKQQRKWSCRIRLDELPKQQPNILCLIPNTQFIQVAQRFGNSLYGDQMIGEPIITYSVSELIKIYKQHFNEGKQFCFILIYIHKISEIEELSIALQSNENQWSQDPDFKKTVMIGVYNQTQLNKNLYQFLKYTIPLSQDAEQLAKSKEWIAEISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NI82</accession>
    <name>A0A8S1NI82_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0890078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NI82"/>
    <dbReference type="OMA" id="MASNDCL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8091822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="161" mass="18713" checksum="A7F2E1758491A18C" modified="2022-10-12" version="1">MQEIEAKKQLKASEGAHFFYTLIFLSASGIIETQFIEQKCNQNLQLFVHLVFYGLIIWGTYILITLIPRYKNAAINLFFNFLDICFGIYIGLLLFYGGRMYMASNDCKSEAPVLYFFLETFLLVNGIIFAILFLAFVSYILKRFSKSQQVYDEGKDEFYDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NIZ6</accession>
    <name>A0A8S1NIZ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0910108</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NIZ6"/>
    <dbReference type="OMA" id="QDANEIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000919">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="23"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="282"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8092748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="351" mass="40051" checksum="50480408A4EFD611" modified="2022-10-12" version="1">MGNCNITDKKDLPDSAILSVHNFEFIEVLGKGGFGKVWKVKRRKNKSYFALKVMSKAKVISKKSVSSVMNERTLLSQLRHPSLINMIAAFQDRENLYLVMDLLSGGDLRYHIGKNKKFSEEETKFFCACIIIALEYLHSQGIIHRDLKPENLVFDSEGYLRLTDLGIARIWRPDNSSDTSGTPGYMAPEVLCRQNHGIAVDYFALGVIVYECMLGRRPYVGKSRQEIRDNVLAKQVQIKRNEMPVSWSLEAGDFANQLIQRKPVQRLGSDNPEAVKNHPWFKGFEWNKLLNKELHPPYIPRSSSNQGGSTQDTEQDANEINMLLRRNSIQAQFDGYNYDPNEAMIAEPSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NMC1</accession>
    <name>A0A8S1NMC1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0920187</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NMC1"/>
    <dbReference type="OMA" id="GWHRSYN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051085">
        <property type="entry name" value="MB_O-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285:SF18">
        <property type="entry name" value="PROTEIN-CYSTEINE N-PALMITOYLTRANSFERASE RASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="405"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="427"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="457"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="492"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8093230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="536" mass="64108" checksum="2A0AD04963668C2D" modified="2022-10-12" version="1">MRQPLTKKSFNISDSSYYISLACIITFEIYSLYRFSIICKSNDIEGTEKGFFGIMRDHSDPQWGEFAGHLTELMIFQFIFLLGSYTVKYNTKLENRQRNLQYYNMILGLGYAFYLHNIGMIFQMMIIISFYLFQKICYKMKYFIPTLWILAILTLWSNETFKGYQFNMISPSLAFLDGFRSNNQLVSWNLVFNMILLRIISFSIDKVWASQLSKYSDQNYNESSNIVKTYRDRVEESQPLKEYNFIGYLSFIFYVPLLFSGPSMGYNAFNSQLKIPQQSYNRAQVLKYILRVYILDLLTFEVFLHVCYPNAIPKLAQNFHILENFSAYEFHIMCVMNLIFLWYKFLTIWRIARGWALLDGIETPENMNRCLYNSYNFSGFWRSWHRSFNQWLIRYIYVPLGGTKYKSLNMWVVFSFVALWHDFKLDLLLWAWIICLALIPEIALQKYFDKEFFYKKWWFVYLCQLGGGFQIQMMCLANLIGFGNGYEGMTVVFYKFMSLEGLLCFLFYWIVRNGWITTIQFRIRDDENAESNDKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NMF6</accession>
    <name>A0A8S1NMF6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0850155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000088">
        <property type="protein sequence ID" value="CAD8090215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NMF6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8090215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="106" mass="12121" checksum="D304B4B7E5BB29A2" modified="2022-10-12" version="1">MKSVQNFQIVQNMKAYFSDDIQSLQKRIDGPITSYDSISSDQMLLPDLKSIILNQNDLIRENYQGLLPTETQSRSLTTNSSSQLFRFEEQFISVNSISSESVSDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPI9</accession>
    <name>A0A8S1NPI9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0810187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPI9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="204" mass="23960" checksum="F14A461121B61031" modified="2022-10-12" version="1">MLDWILLVISGHKPLSRECVYYCGKTTSYQCKEISNLFMSFIGLMLSIVAFVFGFQCVHQLHQYKEYLLDNKNDSFAHQLLKLETRLTQIKILKLSGMVQFGVQFCWDCLAYFLSTTTIECTHYFIPTTLLTVQLYLLQVFIYSLLKLISLVTLPIAVFLVCYECNKEYFGIDHNIWNFSRSHRSLKVLPDREQLKYVEMSEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NR96</accession>
    <name>A0A8S1NR96_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0910092</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000094">
        <property type="protein sequence ID" value="CAD8092716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NR96"/>
    <dbReference type="OMA" id="RLNPVWN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911:SF4">
        <property type="entry name" value="MULTIPLE C2 AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="306"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="112"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8092716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="456" mass="53723" checksum="D49128FD60FF1586" modified="2022-10-12" version="1">MNQFQDVFNEFSQLHNQKYNLGEILSFLDSKCANGYFDRDVYQQLIDQIPQAQSQQQCTINQLINVFKKAQDVLNDKISKCQQIIDQKNIEIRAYNDKIKQQQTNSVNSSSKLNVEILEAEILYQSNGQLQISLECMSSVVYTQLAKRLNPVWNETFDFNVNEQAVMKFILLDTELQQKRGIGGVAYIDINTFGDQMLHDFTVNLTDESNSIVRAKLHLKIQWIYSKNKYLQDLVNENMIQINQLEQEINDHIVDLDIINSPFKQAIKFQNNLTISTYQTQQNQQQQQQQQQGNILLISESQIDRLVQISLLLIILYLVFGLLNTMYRTLNFDFLVIFYCFLFYQKNYKLQSLLHIKIIMVMLGVALLMDIIWLAIYSTPYLGEFNASYDHLEYGLQKYQIILSWLLLFVKIVVLMFYVHIYATYPDKSTQVYDQQWNAIFGWKDIKQINIYRNYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NX14</accession>
    <name>A0A8S1NX14_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PIG-P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1000093</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NX14"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010402">
        <property type="entry name" value="CCT_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052263">
        <property type="entry name" value="GPI_Anchor_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013717">
        <property type="entry name" value="PIG-P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06203">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08510">
        <property type="entry name" value="PIG-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="CCT" evidence="8">
        <location>
            <begin position="90"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="PIG-P" evidence="9">
        <location>
            <begin position="211"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8096100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="339" mass="40710" checksum="481AA1B3CE865CCF" modified="2022-10-12" version="1">MQYLYNYPQFVDDLGQISQELKNELIKFLGKQSVLNLVLLQQQQLLQKMNVLNNYLISNETQSTIQESKLDSVVVISQGEEKINDKNIIRKECLLRYRAKKKMWMNRTLYECRKQIADRRLRFNGQFLNCDEEKKIIKIHKILGNQLRKDKKQQHQNKWHKKNKTFKQRINIRENRQAITLRDQKKNTKQINTVQITLIFLYYFYIFMKSIEIYGFIGWIASYIVFVVYLAWVFLPESALHSLGIHYFPQKYWALAIPSFFVATIFTVITGYAALNYCFCNNFNSYENIEDKYTRLHNLKKTELHEGLPEVYDIPINVVNNVLYWQKEMIEKYLPKHQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NXZ5</accession>
    <name>A0A8S1NXZ5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1000157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NXZ5"/>
    <dbReference type="OMA" id="DQEYQQS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035784738" evidence="2">
        <location>
            <begin position="18"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="113"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="260"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8096209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="366" mass="42680" checksum="05340466E679E491" modified="2022-10-12" version="1">MKLLLFVLFVYTYATSAQYRQAMEDLNDDNFGQTLLETIQMHIQSDEPVGNLVNMLQNLQSQVEKVQTKSEEQHKRISTNCYINLGQLNDQIDNYKIKSVTLKSYIETLNPNVKQSVAMVERKQKEIDDYKQELKQAAQKREKEHETYNNILDNLEQALFGFHQIKQAFNQFIDSVQKGQRKFESFVEMKQILKQVKHTYKLEGYKHIVQLLQSLSKQTSEEEALKLAIQLSAILKQIESYIQTERTREDQADLSRETAYNDYKAQIADLLNEASKSLTEMTGILDSQSNELKQSENEKKEVDVRLQNKMKEFDNIQAECSILDQEYQQSTKQRNQQTKLLEQAILLTTTSLGQLKNDLLKHAEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P051</accession>
    <name>A0A8S1P051_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0990055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000102">
        <property type="protein sequence ID" value="CAD8095603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P051"/>
    <dbReference type="OMA" id="MHAFQLM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8095603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="54526" checksum="19B0B7789186D5BF" modified="2022-10-12" version="1">MHDSKSLFQKMEGTLAEKLCVKELLKEQVIIYIQKTKEFVQKEIVISFNSLIIENQFIELSKCQFEQRVSKPKNSEGFNIIIIKNATCKEFLITSNPQQCLRLKIYLKKFCVHKNYSGRYRFIEQKNCLPLSQSYKKNNQYFSTIKFEKKQLEENQELQIFHNQLTIMHAFQLMPNIFRVCEDAQRYYILGENMKLQSLESLLLNGFEFKEVPFVSIIFMILQTIQKYQAKSIYHGNINLQNIYINTDSQTLQITLLFPKYNEHNTKNIEKDIFSLGKLLYQITFYTQKGEIMKDVNLKLIQSLMDKWSEPNSQRTQYKFLYRISQLDLLNQLLSSKTTVELALIHQWFVNIRQNLKIESKQLMSKALLATIMEEQEFHITSSQFLDNQITITQQSGQDYEPEEELYPIRCQLINTIQMIPSKQKHDDAKLYLYRSKTFASSTIKRDSFPEITKQWTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P1S4</accession>
    <name>A0A8S1P1S4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">cGMP-dependent protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPRIM_AZ9-3.1.T1030088</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P1S4"/>
    <dbReference type="OMA" id="IMAKQYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000068">
        <property type="entry name" value="cGMP-dependent protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000089">
        <property type="entry name" value="cGMP-dependent protein kinase 5-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0140">cGMP</keyword>
    <keyword evidence="12" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="71"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="184"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="403"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8097156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="689" mass="80448" checksum="18264EFB8BEB031F" modified="2022-10-12" version="1">MGCGSSIDVQDDKTTFLLNQANTTITDNDSIYLMKTDTSNRQITENVQVLRQKKSAICINFLTKTLMNHFIFYSLSDEVIMGIINKMFYCCLHSNTILYPKECVASCFLILERGNLQYEGCSLQKGSTIGELSLLLHLEKQKNVKAIDDCFFWGLDKNTFLNSLDYIMAKQYLQNKQFITGQSFYQFLTPMQQDGLAQNIIVTKYRPNAIIAQEGERADSFIVIKSGQAAVYKGTKFIRYLQPRDCFGENSLQMTTKRNATVQAISEVQCLVLTREKVKQILGNDVKKQLQFAVIRQILKQLKTPTLEIEKLIPLFRFHNYNNEIFDINELHDYLVIVVEGTMKSEDKVYHKGDLVSDVYDDQQFYCTGTLAKLDKIKLELEDYVIEDSTYEFKRVRPQFNELNNQKLIGNSSYGTLYKVEYKNEKYVLRKISRDLISQWNLEKQIKQERKILEIINNPFVANFHQYYSDEQNIYFLQDYIKGKNLGDYLHDNGLLDKEKAQFLISQMILLLETFSNLSIISREFKPNNFIIDKNGYIYLIDFKTSKQAYRTHTIIGNPHYMAPEIIEGKGYSTFSDVWSVGVCLYEFLCGGVPFGEDKDDPYKIYEDILNYQLKYPQFYRDQVGKNLMNQLMSLNVYQRVGSSFDELKAHKWFNISWDQLQHKQLKLNPKKQDSNLKRLSNIEMIERC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P2G4</accession>
    <name>A0A8S1P2G4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1000024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8095964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P2G4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8095964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="87" mass="10329" checksum="F7F8E83D2CD729AA" modified="2022-10-12" version="1">MRKFQKLVTQLENQLLTNSIKFFKKKDRAIEQEQENYPPKKNLNQKSEQFYVQLSQSLYKIQEVLRVQLLNIIEGCLLDDIGNQEIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P3F6</accession>
    <name>A0A8S1P3F6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1040107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P3F6"/>
    <dbReference type="OMA" id="CNQIEYQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF2">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="1">
        <location>
            <begin position="164"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8097568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="359" mass="42142" checksum="3144D361719246DE" modified="2022-10-12" version="1">MNQGKQYEIQGLLKAGIDKNQQPIYVMLHNLIIKNKNEEEIAKKFESYLQQFRPFKGFVLIICSQNDGQMNAKDVKHLIKKTLKKVEQIQLKRIDKLIIISNNQNYGKKEKSIPFSLKNLLKEKLHFINNVSFLDTYDIELHQSILKFLCNQIEYQEQQSFYGRDLDSYPIGQSGLPIFLEEIIKYYTQNTNCLLKEGIFRLSGSHEEEMKLVEQLLQQNYEAIQYSEPDVIATVLKNTLVNLKNPIFPFEIYAILRDTNPQIPTNEFVDMFSIFFAHLSKVNRLTVFKLVEFIKFIAGFEKENRMDLHNLSIVFAPCFFRTKEVNQLDYQGAMTVVLHFKSLIQNIDSFLEQQKRLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P473</accession>
    <name>A0A8S1P473_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1040158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000107">
        <property type="protein sequence ID" value="CAD8097665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P473"/>
    <dbReference type="OMA" id="KAYCIAF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="173"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8097665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="661" mass="77810" checksum="10450FFF966F14C5" modified="2022-10-12" version="1">MNCNTYFLERPKIKLNKINQPQCTPKKQCDFSVQKYIKTQPDERTTTNNLSRRLDSSQDTNMILEQRLHWIDQLAESLKSNQSFEVAKILQSLQQMAVTLKQASKNEIELNQKNKLLNKSTLETISQKQIIQQLKEQQNHFKQDNLDQSKKLHLTQMEQQKLQRDYLYQKQLCEEYYKKVRSLEKRIEMILEKNLSTHQDELRMTLSELVKDNEQLKRNVIKREKEIERLKELNQKLIQQNNRLSKKLDNLKTKRVGAKSDNIVFEENYINNYVFDPNQLPQSLEFRLSQLTSEQNDFLIDLIEHGADVFTNQVIQLENMQQRKDLINLIANQFISQREFSEKINQIMNEFITLISYRNIKDFQIHVSKGFKMIFQTETVHLWIIDGMTCKAYTHDANGQQQVALLTEGVFQKIVYENFGIKSPSKEQQLLYITEGSKVYGKNFLLLPIMMNTKKPCGLLEIQNINDEMIIDMQYYGLLVNMLSKSVITSILSYDALYKELKYQDLFYSSFLKLSQSTNFEDFEMKIQDSAYSIFQIAQTTLIHVENNQFKKKGQYYDIRSGCAYQVYKSQRPQILTSITKHLNFDENIDISSILPVFAGPIIKDNQVVAILQFILKKKKLQDQHPFGLQIQIGFKVNSIDEDAQKFYDLVSKAYCIAFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8C2</accession>
    <name>A0A8S1P8C2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1080181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8099124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CEYITEP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035717730" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="2471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2471" mass="285033" checksum="13C848F1EC3DF69B" modified="2022-10-12" version="1">MISFKLQLILLLLILQTKGIDLCREYEQFEICIQSDNDKCKWDETRKLCENTFDFQQGCSLTLNKKACVRQIGNVLGQPAKCRFLQVCEEIPNLEIEKCENHLSKSGCISIMNPEQVCFWKDNSCHVLYQNQWNQVQQDFDQVEYSVSACSLIENYLIIHSNLLWNMISYFPDLFTEANNSFKKEMGLDVSNDIIFDDPNTVTLKNNYQLSNGSFSWYTIRESKEITLTNLQKSYRYRYGCIAIEIENDSDYSKLIALTDEVRGINHLYCKYLNRNPNIAENYVYSDGICKKFSYSDLNNVQKTIEYKLNCKSLDFQQCIYFDSIIHTCRLKGVEYEYSCVNQEDDYVPVCENAYCTVKQCSQQIFHYLDTETLLCNNKCSLFMLNTQSSCEAIKGCKFLGTSVIQMQKTCSPQNGCDQLGIEKMYCKLLKDQCGWNEQQQRCYRIKDEEFKLMKCNQAFNAQSCTLITLSDQICYWNTNSLECLNVLEQPILIYQKSVIDKDDTEYWNILFQKISNENLCKYQQTIPTEYFPNKGLCYFFHYTPETSDQNHINLYSCLKLQSGINKWDSLTQKCISLQEIDLKEFDCNTQVLVNEKVCQKSKVSSSDYLCIFDSYTNSCYEQKLTSIQSFRCDGYGFTKEVCVNLKGQGVYCKFSAGICQTISVDEINSIRCSSLQNVNQRVCNLHNLQQTVCLYDQSINSCYTPSIYAKITYQTEINRYGCQLVQDSLTYFDLNLNECIQFSQDDNILLSNLECQQNYVNQKTCLSIIKSGQICYWDYNENKCKNYSRNFSNCQDEENITSKFCQVLESDVSKQLINDNYCSFVDNKCQAKSTYISDCGITENMNIHRCSGLTGLNESGEYIQMCAFINYLCTPLIDDSMDAFLILNQIQCLQANLKACTKVQTNGQYCKIIDYVSNNHFIIDKKCIQINDKNQPCDTINTNFLKKQINPNHCSRANDSCFYDQSTGCQMPINDDLECDTLGLSYFGCILNTQNHRCAFYNFKCQYLPQHQTISDCKYLNQFTCSNYQYLKCYWNGQMCDVTFDYFMEYGGDFTCILDEYNDFLVSDGSTCIYEEDQLNHQLYNCDSKLNQFACGSIPDQYCQFIQNQCQFYKSTLCEDSIQTCNFNNSINLKCVLKDGKCFHFPYTNFTCDFFEKTNYEFCYQYPNCVYADQKCQQIDQTQDYWYCYYLSQLDCIIKNKQYSCSWEYNECIDFEGITCPSLNGYHSFNSCTQFPNCIYGYTKSGFGFCYSNTDFDTLTCEQLNQNLCLQDLTHINSSLLCYWDQSCKNINTTQITQCEDLQNMQSSYAACAQLNQDQCMYSFIDNKCKLISEYLSVTSCKGTTSKQCSQIKNTCYFNGSKCLTTGVPEQLNKFGCINQSGTWKFSYFTCSKLTNEMQSSCTNLSKQACLSDLTKEISCQWKDHNCFNLLENENKKLISCQDLNQRACQNVSLSNIQCIWNLTNKICDQITLTNTECSNLNNLDPLISISVCSSQTTKSCVRHYDKTKCTEINEKLNNCNLYGLNLQACMQQTTIPCTWVQLNDGGYCDDANLYIALCQDLLNQSACINIKTSGQICKWDVSTQKCINYIFTTCESAHNEHTFMACKSVTEEACSYNHLQMSCNQVQYIPTTCSSDYNYQACMLSTDICVWDSNRCIYNPNQNCFKFNTKIKCLESDQQNCQWLENQCIDFDPLFVKVYCYELPKNINNYACKSNSIEPCIYDSKLMQCFASQEKMISVYDWSSIEDIVRLQKPGHQSSILHGTCEQLQNIYYCIKSRIPNKSCIWNDSCQEVIDFNNISCSDSLNIWGCLNVSNNNQYCIWKDQKCQQWNSTLGIMENVNRNVCIQNSVNSVYEQNSCIQKSIESILCTSDGISKLTCLSIPNQQCQWINSCINFILTNQKKCQDYQNVNYFVCQMITDLTCSYDFSKHICVDTSNEIIGLGMSKFGCISNKNIPTFWNNSHGCQELVDYIDCDTTLIVNEFACRKYVKNKACFYDSITNQCKSHFNIWQLQCKTEGLNLLGCVQVQQEPCIFKDNKCQVFKESSSICMFINLVNSKACASVINQQCSYDSINFKCQTPLINENYCNISGINKDACEINALCMWNQDNLDCKCKSDQKIDICQIFNISECRSNSKCYFDLDIYKCIKRQCYHQKESECSAIMDNKTCYLNQKNGCQPATKCEDIIDPQQSCSTIYINSQPCIQAGNSCITSNNYSIYCPYSDCSNSNCIIQYGACKMKTCDDYTSQECQLIQGCYLDQENNCQQLLRCSQITQEIYGEQVSNICNIASVGGLICSWQRYTLLDDTEICTNQYCEIYGSSTTLCQGNEFNGFSCVLLNQLVCKQCEYITEPCFCNQQKNICKYDNGKCNSILCSNFLNKETCSQASDRCYWSTQSENDTEINLCLKQCEKIIDAVECNSRLNECYFEISNGLCVKGQKQILDLSSEILIEEFYALIIQGYIFLCLIIFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8T2</accession>
    <name>A0A8S1P8T2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar ATP synthase subunit D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1090009</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8T2"/>
    <dbReference type="OMA" id="REEFFRM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8099163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="28769" checksum="820ADAB79AE910CA" modified="2022-10-12" version="1">MAEQVVPSRMNLALYKAKIISAKKGHELLKKKCDALKTKFRIVMVALLENKKSMGDEAQEALLLIAKAQYAAGEFHQNVKDAVKRATIRLEISSENIAGVMLPEVNIREVDDSDSSMSQIGLARGGQSIQRCRDKFKDLLVLLIKIASYQTSFVSLDQVIKVTNRRVNALEYVVIPRFVATMNYIDMELDEMSKEDFFRLKKVLDNKRKIIEKQNQETAKRQLEVEDMNWQERESYDMLAEEQPDEDVFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P9Q9</accession>
    <name>A0A8S1P9Q9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1100008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000113">
        <property type="protein sequence ID" value="CAD8099521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P9Q9"/>
    <dbReference type="OMA" id="QMSMQDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000005">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027040">
        <property type="entry name" value="PSMD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10223">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10223:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="7"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="297"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8099521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="362" mass="41595" checksum="23A9DDC40D81D2C0" modified="2022-10-12" version="1">MPLEAAVVVLDNSEFSRNGDLEPSRWNAQQEAIELYINVVIDSNMESGMGLILGGGKQVRLLMTPTNDRDLIQGQFHKTRLEGNLKFSVALQQASLALKHRINKQQHQRIVAFVASPIEEEVDTLVNLAKRLKKNNIAIDLINFGEQNEEHLQKLKIFFENVQKGSSSKFINIQPGMSATETLFSSLGNYSDFQAEPGQEQQVPQQRTGGQFSEYGGVDPNKDPELAMIMKMSLEDEALRLQQQLAQQQQQQQQQNQQPQQQTQQPQKEQIQEEKIENFIEEENDQLLEQARLLSMQVEQSEQPEQPEQKNQNPQQQQQQNQITQKQLFQDQNFLDELLDDVNKDEEQESLLKKDNDQSKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCG9</accession>
    <name>A0A8S1PCG9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1140055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000117">
        <property type="protein sequence ID" value="CAD8100992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCG9"/>
    <dbReference type="OMA" id="LYNIHNP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8100992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="267" mass="32191" checksum="69FFF0A83DB93FDA" modified="2022-10-12" version="1">MSQLTNQLDDLYIQTQRLFLLRQDSIIQNYDQEDELMDLQQNQGGFQELFYEFENIISPNEDAKKQIYFQISDNENNVNFDLESSIQISDNEKNVNLYSEPSIQMFDNEKNENFGLESNIQIINPQKTPEKIPKKKFNEKSYCRYILLYLFRTIENKQYAEIITQICSIYQINYSNFVEYYRKQRILIMGYQSLKKELIYDKDSIPDQNRKKAFKEVLIWYLDKLATKQILLSKKQNFEGYLKFKNYVMSYYIHTPKSWAGNKPQWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCQ2</accession>
    <name>A0A8S1PCQ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1120172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCQ2"/>
    <dbReference type="OMA" id="NTIDYFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000289">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="73"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="231"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="617" mass="73197" checksum="642A88FFF9EBC567" modified="2022-10-12" version="1">MSKQNKTLIFDQAARHARIKKENEEHLSQTLKLYQEYGKIENNELIETVIDSLSTIEFFKQNCPEGMKLSEFVTNAIKYFQFEQYDYGQPIFHYGEYGDKMYIILKGEVGVFGPRPLDDINRDIEQVKKLEEIELVKVQKKQVTVNFDNLLKMQGDKYYKDGVLLYQKIFQYYSGQSFGDVALTSDKPRSASIIVYSEKAFCITMRRNDFKLIFQKSIQQARNTIDYFMKIFPGTLKFNISKFIQYLRPIVFHSKTILWQIGDEPQFFFIIVTGRVQLYKYFDRDTLMGKKKNQNLSYDKLYLSKQSNKQKIILSQLSDGSIIGQEELIEDLPQRKYCCEVVDNTNAYYMEQSDFRAIQGNHPDIMDCLRERIQINKDYINKRQRLILDNFKNYDDYLTSLNHNLNSQSQMKDLFLNINNKQICIRSQKNLKVSQNEILLQHIDYDQKRKSLEPEEESELRFIINNNNLQQIRDRFVRQQQNERECLSQQKQAHNRKLITQILKKYQYKKIQHEKKDRKRNSIFITSSQTSIGQTIETENSVEILRQQQMQRSSVIIPTHVKLRARKQSNQKSYNNNQTLVNLTEMESRTHQQTNITHITPSTCKFFKTIRSISGKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PGM3</accession>
    <name>A0A8S1PGM3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1170118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PGM3"/>
    <dbReference type="OMA" id="RQGINVT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052637">
        <property type="entry name" value="KLHDC3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46461:SF2">
        <property type="entry name" value="ATTRACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46461">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="404" mass="46054" checksum="316CC76EC5CE9CAE" modified="2022-10-12" version="1">MKQEQNHFTFTPVIHPPFIDLEKQEEQFYPSIGYNFATGDVKPGWAFISKDQPPCVPYFSSYIKKQNGAEFLITGGAYQENKQIFSSTFCFRIQVHDHMEGHRIKYELWRQGLIREDEEGNEIEGEKVPVIPQMNFARHNHKVFELLKPGTTNQYLYMVVGGQLINQEGWGLLNQCEIYDEKGPVNQNGEQQWEVVDSMIQARAGFSGFVMNNKVYVYGGFTIENEETKILHSNKVECFDLMTMKWKDFKYRQGINVTNHLGSCCLPISDKHILVFGGSNGEKVTDKIAIMDIEKKSIENIKSKTKLSVPRAGSQIVVLKQVEVADSAQKRDLIVILGGNQDEVSFDYFTIKDQNAVEIEYVGKNEFNNAFTGAVTGTYAFGGVNDIRAWVKTMQYMPVVTLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKH9</accession>
    <name>A0A8S1PKH9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1200107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000123">
        <property type="protein sequence ID" value="CAD8103244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKH9"/>
    <dbReference type="OMA" id="HHVDDSQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8103244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="367" mass="41608" checksum="30F18F45C7EDFABB" modified="2022-10-12" version="1">MYNTQPQFQNIVQSQRVTNDSLMDTVSEIRWNKDVFVTSSWDGYIRFYQVQLQSGYRANIQVDFKTAIDCQEPVLSVSWKQDMSMVFAALADNTIRAYDIKTQNSAIIGIHDDCPARQVFWNEDMKLIISLGLDKKIRFWNLQSSGGGKPQPAFQLDLQHVPTVGEQSGNEQFFAYADVDNKFRWLHWSALRGVQNSVASRSFFNMEDNYLIGQISCVAINENASQLAYATVDGRALVKSINSRGDLASKLQFKCYKVDEEVKVSQYRTEKVSRMYMCNAFQFNCRSQNWVSTCGSDGTLAFWDTNKKNKILGVKLDGPVVAGQVSQDGQIFAYATGYDWAQGLDKVGEFTNRVGAAYIEDKFLPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PPZ6</accession>
    <name>A0A8S1PPZ6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Receptor expression-enhancing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1260061</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PPZ6"/>
    <dbReference type="OMA" id="DTQYWVV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF161">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8105122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19496" checksum="24318F651E9BBA5D" modified="2022-10-12" version="1">MNILLEKLDPFVSKCGLDNMDQIEILKGPCKKIGVRPAHVVFICGVVSLASIVLGIAARFLSTFVSMIYPAYRSIKAIESGEKTDDKQWLSYWILFSLITLADSSIGFILEFIPFYHVIRLALFVILFHPSLNGAEKVYQALVQPVYQKYHKHIDQQIDQMSNKLKNQNIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRP5</accession>
    <name>A0A8S1PRP5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1270009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRP5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8105313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="141" mass="17236" checksum="22EC12D2A93FB8CF" modified="2022-10-12" version="1">MKEMINSDKLEAAWPQLKIFKNLSLEMQQLLKTLRLVLSQKKERIWEQHQDRQKIIIFLDFDELLFFYMFYLSKRVNNNYNISELQLDDEYKHRKIIFQSKILSSISQFDLINYQLIQLRINYQQLKVILQSQYITNSPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTX0</accession>
    <name>A0A8S1PTX0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1300124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTX0"/>
    <dbReference type="OMA" id="QVKIWND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="70" mass="8219" checksum="5D1AE5AE63811AC5" modified="2022-10-12" version="1">MNSNSNKDVQVKIWNDEIEVSQEAQTVKKTEIYPQRLSFLVIETSNDELSYRNLLKYKCALAKRIKKART</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PU43</accession>
    <name>A0A8S1PU43_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1320007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8106837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PU43"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="57" mass="7008" checksum="D650CAC4BBADC84C" modified="2022-10-12" version="1">MINHWKNSNLLLKEKKSMKHFLVSQNSLKQILEFNFLIVQLFRSSLVLWDHQKFYMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PX28</accession>
    <name>A0A8S1PX28_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1340082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PX28"/>
    <dbReference type="OMA" id="TPQFFKQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="357" mass="41883" checksum="BDBD846568FD1FA6" modified="2022-10-12" version="1">MQFQNHQNQRQSRNRIFDTPISESLSLSRNLSTNFTPVQCDENQKQFIKTSQFTISTPVDQSIQEAQFQQPIQAEIVFGIQNTPQFFKQNNKVIRRELEKYIDESGQLKIDEGQSDEIESSEESLKSFPENNISNFERKESHQQQFYTKNHENQQEHTLKISDLKSEQILKDSSIQENQLDSNSQRITQNIFSVQKTQIDQQSAQSIQSVKNENQDLSISNVQISQQIHQQSFDQSNISQWSIDQIEILIQGIQVTKYSRKTLFGKKRRILQVSPDLKLIIFRNLEHKITKIYSINKITKMEKGANTETFRLYKPKSDILWLCFSIFLDERTVDLSVKTQEQLRQILDCLYGLIQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXX8</accession>
    <name>A0A8S1PXX8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1350095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000138">
        <property type="protein sequence ID" value="CAD8107941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXX8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="150"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="63"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="175"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8107941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="196" mass="22797" checksum="EF7A80A9678EBBF7" modified="2022-10-12" version="1">MRKKPFKDNGIYLEKHAKALLDYQQTSDEEDANTQSKYRIKAIKYKNNLLESQEVIKALKIENQESKKIISELLLDIEEMKEKEKQLESELKEQKQLTQSAIEQLEQELQESVEDRLLQEQKQFQEKLTIQSNEAIEKLKKHFDEQLQIIAQTPKATAPPSKKKKKKQTSSSTSNLRPQSQNTGRSPASVSKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZB5</accession>
    <name>A0A8S1PZB5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1370057</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000140">
        <property type="protein sequence ID" value="CAD8108432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZB5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="70"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="360"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="398"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8108432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="57169" checksum="FD4B3BEBEE086A56" modified="2022-10-12" version="1">MGACQMKSKQKTNEDQPQSSTTRKSDQVFTLNNPSLIIQTATPNLENINEPYEETKKQLRKIKENQCSYIRYYTRIQQGNQKLLNHQQLVQHNLTGKIRIVEVYERNSINEQFLTELESLDHPKIITVHEVFITEESFQIVLDYCSGGQLSDYCSDKSIVPFSESDIASIIYQCAEVLQYLQNIGRTHGHLTLNCFSRIDTSNDIQIKFTDIRGLYIKHQINNINIYTLAPEIFNSTDDYPPSVDVWSLGILAYQMICGYPPFGGSTIQSIKQQIKKMAAQYNNFQFDRVSKSCVQLIKKILVPFKSRISLSTLMNDQWFKLRSIQNDSNIIKKLQQNKVKISPLQQLFLTFMIKNFCSADQQQLHAEFLLLDENQDGQLSKQELLKVYSNRFDTNSEAILFIDNIFKIADIDKSGTIDFGEFIVAITDKSGLLTEDNLKMAFKTLAASNGKLTRQALRYHFNLTIQKIDKLFADHNLDVNNVGYSEFEKLMKQML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2M5</accession>
    <name>A0A8S1Q2M5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1400120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2M5"/>
    <dbReference type="OMA" id="PFERSMF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="12"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="171"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="383"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="476"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8109392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="537" mass="63159" checksum="D81C1385A7426C91" modified="2022-10-12" version="1">MSKALQYNTISKGDYLTQKSIPNSAPMLRPQSEMVKQSQVSPPVQAPTDVIASQPIYKEKQEKQQKDTPFEKSIFPQSEIPQQNIPIKSTMMNNQQQISSQTNLPQQQSNVSNQNQIQILQQLKGLQLENQELRAQLINQTREIMNAGALKSENGALRERADELKIIADHVKEADAQNQAMKKQVTELQTQLSQIKAQNGELQKVADEVEQVRKEIATYKAFEMQYKELLRQTLQLKSDHDALNKQFNKLLSVQDENKRVSQQYDQQVTENEQLKKQLVQQEKDNQLLLNDNISMKQLLDQLNFDHRSLRSQFAKLQQQQQQQYEENQKLKDKISQLELEIVQLRSRNDGLQKFVTAVDTREQEIGTLKYHLGMCENNMKIIQQNYDASLKKLEIQRKDIEQLTTDNNLLKGQLEHLTLQNRQLNELQWKNKEQDHQTKQMSEEIKRLNSLILVKNNECNQYKNDASRFHSQLMNLQNTENLNKRMNDQIQQMDQDIQFLRKMNQDQQMQIKQLEEFRYNTIYNIKQQDFSSKQNLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8X7</accession>
    <name>A0A8S1Q8X7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1490051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8X7"/>
    <dbReference type="OMA" id="IEHWDIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10537">
        <property type="entry name" value="SET_SETD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040415">
        <property type="entry name" value="SETD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33524">
        <property type="entry name" value="C5ORF35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33524:SF1">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="48494" checksum="A1B03893496716EF" modified="2022-10-12" version="1">MYRHLYRNPTILSKILIKEYTNLKSEELAFRYAALAKLRTQELEFEALHQLDPIEHWDISKISLLPQIFTLLANLHRATRSDSLIQYPQIEAVNRITALLDPNMFPVQDLSLKLHTSDTMTIWEKSKTQRVTKNLGFKLNLRESTIDHSDAGEGVFLETLPANKRLVPAGTLLGFVPGLIYDNYKSYGEKTPPDMHFFRFDGPIFDFTSKIFYPYVPGFGYDEYEQKIQEIDDLAARSNRPYRNKSKGGYELVKGEDINPYALGHKINHTPRNKSTNVCLIDIFVPKNFFPEELMKFWPTQYFSTTQDFLQSKSILDNHYKNHIRGLGFISVTDIKDGEELYLDYLESCLFNMEIESPDWLIKPPPLHPQICKCNLRQESLLMMLLEDYAFQQTKDGKNFDKFYKSVLRENHQYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QRE9</accession>
    <name>A0A8S1QRE9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1990010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000208">
        <property type="protein sequence ID" value="CAD8117445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="TIFTHIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1323"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1378"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1434"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1467"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1518"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1628"/>
            <end position="1645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1657"/>
            <end position="1675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1791" mass="205823" checksum="11DACDCD3871EC4D" modified="2022-10-12" version="1">MQSNKYLNQILNLELENYRPKKFQFKITLLLIHQDRLEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGLLLIDCPNCQIYLMNSNFQNNIVLNSISSIMNIKTQKLQICNSLFYNNSIFRYEIIQPHIQWGFTTSVSEYVIKQIFESNSKSGVGELQAEKILIQNNTFINSSGSIGGCFSIFSYGNTDILIKNNSFEGISTKFLNDLEYGGAIYIDGSSSSSLNIEFNNNIGNKIFCRGFGGFIYLKSITSQSNLTIINLHLEDIFAQQGSAIYVSFSKFVESPQILLVQKVQISSTQIGYLEFLNKFNEFSSQQEIQQLINNRSLVYLEFGSLIQMSEVQTNNLILESFLILQETKYIYLSKIQIQNSSISNKLISVFSFTTTNQEIIIQNSVFRSIQIGFNFTNLTNKTCININPQLNDIMYQCLHDFIQAPIKLNVNQQSGYYNYAFCVFQEIIKQIDYKNSGLILFNNFTNQDKIQISSLTFVDIFCSICEYGLIYLQFQPSEYFIQQQLITSLKVQNCSCGKQGCLNVVKLQNKDRRLLNQFELYSLIYEFKIRYYYCQHNNATQGTCLKINSLQTLLQNSLFQYNEAAEEGGSIYIKGNKHLIIEDCVIHFNKGSIGGGLYFSDQVAMNLLNNKTKIIKNIARIYGNDVASNPQQLSIQIRNDSQFLYAQTKIQNSTQKIQEINLNPYVDLNGQIVKHIYLPTGQEISSYEIFDWKKKLYYKSNMIFRILALDGDLNSIQQLQNSLCIIESRQFNESNNEDQDFTNNYTSIQQISFNQVTQSYNLDDLIVYFDNSVPKEIVLQLQFSCNSIKVPIYNTQYPYQVIDYHSNYKLRVNVKTYQCQFGEIKNTSNFSCEKCDSDQGLYSLTLNSQKCDIKDDLTTLSIKDNQLILRAGYWRPYFDTKAINYCLNLQENCLGGINEGDNSCYIGHIGALCEQCDLYNIRGNGQYSIVKKFTCGPCVEKEKNVFVILGIIILTLIFLLISIQGNRKTMEEFTKSSVFKIYRYQVYLNKNQSGTLIKMLTNYFQIIVSITTFQLHFSAGLNNTLNVVGNPLQTSSYSLDCFLVEYKDLQIEYARMIWQIILPILYIVVFICFYFLAILIKKDRFNPSIAATAAIYIYIYVQPNLINGFIELLSYRNISGFKWIQANVSKRYDTTTHTMWMFEFCLPFIILIAIIIPCFFLFGLYSNKNHLEKKKVMSHWGYLYNEYKKEAYFWELIKIIQKELIILSLVYYEDTVVVKGVLVLFITYTYQELNALYQPYQLNNLNQLDYYSANISMITIGLAIGTYMSQQVHLAELQIPFFFLMAFQNLVFLGKIIKKILQEYSKQYEVLLDKIKEMIKQRFPEIDENPFMKRIFKSRGEARKHAILRFSKLRNFGIPLAQQIIQMRQANFDQNAKKTISTSTAQDGGFLSKKRGVYYQKSLLKIKSFAKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JLU9</accession>
    <name>A0A8S1JLU9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0040263</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JLU9"/>
    <dbReference type="OMA" id="YKRRSTH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10531">
        <property type="entry name" value="SET_SETD2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000066">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006560">
        <property type="entry name" value="AWS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050777">
        <property type="entry name" value="SET2_Histone-Lys_MeTrsfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22884">
        <property type="entry name" value="SET DOMAIN PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17907">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00570">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51215">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AWS" evidence="16">
        <location>
            <begin position="66"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="14">
        <location>
            <begin position="129"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="15">
        <location>
            <begin position="253"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="286"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="362"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="990"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="364"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="999"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1015"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1025"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1074"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1101"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1144"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1185"/>
            <end position="1198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8043043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1198" mass="142609" checksum="E7E58F4AC5E2E5BB" modified="2022-10-12" version="1">MQMDESNKTKKNDQPILKETSKNRNIINKLQGNPSRLQGLKESFKYFTLLKKNQYLERKQFRVSRDETLVCSCICCPENQIGSRIQGPQYSYNCGDRCLNKFTCTECDVELCPCAELCKNRRFQKHEDACVYPLKCGGKGMGLYAGERIQKGQFIMQYIGEIFQINSALGRRRVQEYSKSTCTYLMKLNNQEVIDPTSKGNLARFINHSCEPNCITEKWNVLGEVCIGIFANRDINEDEELTFDYQFDVFHTPLTKCLCGANKCKGYLGLKPTDVTQEEWEEHLENMVCKICQTKTPQDDEQLLLCDKCNCGFHLLCLVPPLESVPKDAWYCQECQDEKRILEEQEKDKTNIDRIQQKKAKNYNNEKKQKKKREELSSSSCSDSDSEYDNRYKMMKILEKQTVREYLSLKQPQQYLSIRQDEELKKDEDNQISEIQVSIPGTSYFKNDQFIKSSIEVQPQDSGKLQQMQSIVDVAQQKFEEQYKEKLELHEQIEAIQQQNILNIDMIKECFKINILEQKVVKKNAQLIYKIGNKISFEQIDQQFADFFKKENNLTVIGSIQQINIFRSILNMVEHIIKELKKELGIIEGQIKVPIIYLKRLINKFNSLDNKQEVNIIYNKSLAHSEEIFPMDKATPIKIKGSKQNIEQTVSEIRNTLKTLCVQRLYISRSETKQVQQNMFQLKQNAEIRISRDSVYNARGEQTQRDINHPFFYIQYREKEVCLIGTLQQIQETSTAIKKLLEQEANNEKEMTYFTVLISPQYKESCKQVKQIIERSSSSTKVLLFEASHPRKNMTILVLCLRNQIIQTKQTFSDLIYQQCQLKLDQFQDQMSMQMCRYVFKYLQNTMMTNDMAFMKNWDLITPYFYQFNIMKYRESKFENWFVKRCYSSLLRDYETQLYIQYCLGKEIQKNLTDHEQFLRKKNLIQLTKKILQAILGFKRYPSDQITLDSGQQQDTIKRFNNQQEDDNLKLPNLQLITLNPQYTSINKKSDIKKEISERSQSSSSKYQKKQSNSESEKSRDFSTRQKHKYHHHHHHKYYNNNQKSPTEKYIRHKYDKNDYYDKQKSRHRYQYQKQDKSRDINKRYYDDDLSKNRFHNKKRSSSEESDKSQEQRRKRQESVDSDKYKNNKYYSGFQYQRRHPKTNKYDYEKSSKNFEIKDRSRYQSKDRSRSKQRNKSESSDSYKRRSTHKSKKYSRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JM79</accession>
    <name>A0A8S1JM79_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0040546</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JM79"/>
    <dbReference type="OMA" id="ERTQWIY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="209"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8043322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="35642" checksum="F5C5DF60970D9155" modified="2022-10-12" version="1">MSERTQWIYKSKEILKPILETQFIGVSRAILGLPMDHFFDRFKTLIQASQNNNFKQLFLESYNRNGIFRGIFAGFSSQISIQLFKQYYRWPMMILIPRLYMQILPEKIKESAPALHKGLAGITIALFESFVTCPFERVKCQVMTQQQSKSVLKYMWEHDGGIKGFTRDLFTGMEVMVLKQVVSWTNYLYWDHRVRYYFKRHPSQKLTVKEIVLCSIFTAIPNIVIVQPFDMIKTQFQMENNDQIKNLSIWNAFKKVYYEKGIPGFYAGWQMRFCQFMFQALLTTPVMDYLERLHGVAQKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JN00</accession>
    <name>A0A8S1JN00_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0040384</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JN00"/>
    <dbReference type="OMA" id="HSNDEEH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035844364" evidence="2">
        <location>
            <begin position="21"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="252"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="385" mass="43798" checksum="CB93C1F55D4D4C38" modified="2022-10-12" version="1">MKILVAIVIALCLFTHPIVCKKHQNDEEHSAFEKSHKKLSLQDDPSYSKLEEIQDHPLGSKILQTIALQLRGNESLSAVSKLLNDLKGDLEGKQIDADNERAQIGSQCKKDLQNYSQRISLSINEIKDAEFKAKRLNEAIAVYQAEINEKARQIQVFQAKDDTLRDIRRQDNLDFSTRTTQMKEMVQAFEVILPKLHQVWDVAAQHKAGSFIEEEAINEALVQLAKIGEENPITAMVALTSTLEPTAVQTLIEKMEAIRDSIKESIKAEEEAEAKNARDTDTILAAIFNAIESLTREKSSDEEALQETIRNRDIQDKRSRDAHAEFNAAKNGNQQRNQQCQEYELQYQQNTIERDKQIAIIRDVQNIIATKIEVVTCFVEENNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPI7</accession>
    <name>A0A8S1JPI7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2HC/C3H-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0060073</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8043991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPI7"/>
    <dbReference type="OMA" id="EYYMPSI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026319">
        <property type="entry name" value="ZC2HC1A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049899">
        <property type="entry name" value="Znf_C2HC_C3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555">
        <property type="entry name" value="C2H2 ZINC FINGER CGI-62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555:SF36">
        <property type="entry name" value="ZINC FINGER C2HC DOMAIN-CONTAINING PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13913">
        <property type="entry name" value="zf-C2HC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52027">
        <property type="entry name" value="ZF_C2HC_C3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2HC/C3H-type" evidence="8">
        <location>
            <begin position="191"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="229"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="229"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8043991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="283" mass="32775" checksum="672CDE1B8AA2C676" modified="2022-10-12" version="1">MNYKPNLKMDSTSGSFKKQEQTSSLESTQKSEYNGISIKPNFACKPVFNFRNRSPQPQEQEPQLTNTNDDFFKTGTFQEKIPLKDRIQRSDEKKNTAPVAKGLYLPGQEELFRKQIQVEKKEIQQTQEKKDAQSGKTIQVEQYMPSIGQQSNKREAALLGSKNPLENKIAVANQERVLQQLEEAEPIEELELILCPEGCGRQFKQDALEKHVKVCKQVFQQKRQEFNSKQARVVTNDQQKLQRQGQIKEKQLIKKQGKAPLDPNWKKQSEELRNLIKESKQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPW6</accession>
    <name>A0A8S1JPW6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA helicase</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0050471</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPW6"/>
    <dbReference type="OMA" id="YSGFHGR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12937">
        <property type="entry name" value="GUCT_RH7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001645">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059027">
        <property type="entry name" value="DD_DDX21-DDX50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012562">
        <property type="entry name" value="GUCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF19">
        <property type="entry name" value="NUCLEOLAR RNA HELICASE 2-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26142">
        <property type="entry name" value="DD_DDX21-DDX50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08152">
        <property type="entry name" value="GUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="56"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="262"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="584"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8043817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="664" mass="75771" checksum="4B65D31D29070C35" modified="2022-10-12" version="1">MLTRRKSSELIEPDIVKKIKGNDESNLKAYIKNKQTRKILESRGITNLFPIQQQCFETIYNGDDIIGQDRTGSGKTLAYCLPILERIRGLGLKQNKNPYVLVLLPTRELAIQVTTEFNSILHKENEYRIYSIFGGTDLRNQIDQVRQGCEIVVGTPGRIQDLLERKVLKLDEIQVVVMDEADQMLNFGFQENIEKIMSYFNDRKIQMLLFSATIPDWVKELSQKYMESNTKHINLIKRNETQTSTTVKHYALQCARNQLTGAIGDVVSVYGGRHARTIIFCETKRECNEIILHSKLPAETQPLHGDIPQQQRTVTFEGFKNGKFKCLVATNVAARGLDFPQVDLIIQCNPPKDIESYIHRSGRTGRAGKDGICITFYSKKDMSLIERVERVAKIKFIKISAPQHQDIIKASSRDLQTSLQVVSKEIVDLFQPVAQEIISRCDPVEALARALACVSGYKDKLQNRSMLGSFEGYITYILRSSTPFQACGYIWKFLKNNFSEQICNSIKGMKKLRNENGVAFDISEDVKEEFENQYQEISQNGYCRGIEIEQATTLPDIIEEQFQSHQNYHQQPTVQVSQTVKKEQEIFIGGLDFKITEDEIKNEFKNKGVELFNLRLLRGQDGQSKGSAFGVCRTKEMVAVALKQNGTKFRGRNIRVNMANEKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPX7</accession>
    <name>A0A8S1JPX7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0080117</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPX7"/>
    <dbReference type="OMA" id="FILHIRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052983">
        <property type="entry name" value="MFS_Riboflavin_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43385">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER RIBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43385:SF1">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER RIBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="379"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8044553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="52411" checksum="0AAE960D5F711C82" modified="2022-10-12" version="1">MFLSESKKCLIGAVLIKIGSALLLTWGKTNVYFYSFYKQTEPDLSIAYNTLPIAIMALPIALITIFSVKIAKKVGFILHIRIATIIQCASLLIAPYMPTYLTFLIFYIFFYGIGYSSVAFPVFSCTWSHFKKSEGRVSGLLLGAYGFASVIYVIIITYISNPNNQQADLITEHKTQTIKYFSGNVAQNAQKAAQIVSYVASICCLIGGFLIRPYVETTNYNQVGVSDQKIQQFEEETPKQHEKEAQQDSSVEIDPSSVAVNDLPLEIASDHGLPQIKIKQSLSAAIKSIQFLTIVSCLSVLTLFLLTLGLNYKSYFLTKINEDFFITFVDLVALLASSFANILWGFLIDRINFKILFVIIYFVSGIGAIALPFSATDKISFFIVYVVTMMFDKGALVVTGPALIKIFGDDIGHQLFPLTYISSLVSVVVGPISQFILLKYFSFDIILYCQGSLILLVIIFIIRLNMNYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPY0</accession>
    <name>A0A8S1JPY0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Actin, cytoplasmic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0050242</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPY0"/>
    <dbReference type="OMA" id="WRYIFEV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000007">
        <property type="entry name" value="Actin like 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8043589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="370" mass="42334" checksum="FE43C10332772FF9" modified="2022-10-12" version="1">MIESHPPVVIENSSCNIRAGIVGEASPRCCFPTRIIKVQDSKAIKFLIGSQIEENQNNNQPCYPIQNGYIIDWEAMEQIWRYIFEVQLKISPSNHPILLTEAPRNSKMNQERMTEIMFENFDVPFLHISNQNVLSLYSLGKTTGVILDSGEGTTYCVPIYEGFQLRHQLSRLDIAGSNCTNYMTQLLKEEFLKMYTPFQRNQIAKAIKESLCYVALDPIAEENKYLGNTSHNSSYILPDGNKIQIKEQKYQCPEILFNPNKAGYELPGIHEITYQSISKSDRDIQQQLYNNIVITGGTTLFPGLQERLLKEIENLVQTPSQTRVIVPPNRLNSTWIGGSILSTLTNFKPFWITKHEYEEFGPKIAHIKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JR17</accession>
    <name>A0A8S1JR17_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0060131</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JR17"/>
    <dbReference type="OMA" id="MIGFGRP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000059">
        <property type="entry name" value="N-ethylmaleimide reductase, FMN-linked"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="4">
        <location>
            <begin position="7"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8044049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="368" mass="41106" checksum="C55E07D76B36C8F3" modified="2022-10-12" version="1">MNNTSILFKSLKLGELTIPNRIIMAAMTRMRCDNETKVPNNIVADYYSQRASAGLILTECTLVSQRSNAFPGCAGILNEEQVEGWKKVIDAVHKKNGRIALQIWHSGRATHSNLQGGQIPWAPSPIAINGVNYAVKQPHEVPHEMTIEDIKLVIQQFKEGAYRAKKAGFDAIELHGANGYLVDQFLRSHSNKRNDLYGGSVENRSRFCLEVIDELIQVFGKGRVGLKVSPLGRYNDMFDEDPIKLYTYLFKELDKRQIAFIEIMNSKDEDNCYGYGHPASEKQVPNLLQQLRPSFKGVIIGNVGLTALTAAQGIEQGLFDAASFGTLYIGNPDLVERFKDNLKLSDSDPKKYFGGNHEGYSDYPPFQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRR8</accession>
    <name>A0A8S1JRR8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0080460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRR8"/>
    <dbReference type="OMA" id="KSEWYEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="461" mass="54011" checksum="C408EEAB18E92CDB" modified="2022-10-12" version="1">MNQEISKEILALSIHVAAAASSITVQTQQRMTLVTNSVLETVSQTMKIINFLMLCSQKQSQLEAVTEEQEDEGQKDSKTKECLNEQFLKTEQSDCFNDEVIENQKEKENCPTDMIMRQVSELRGQSILKSNNNLSEEYPKCNVYQVESATPIQFRKPSLLSQQLSLIKVDKQKEAEEQRELMKLIESVDPIELIPKLKSIEIYDEYKKSSGFKDILVYSNKQQIVINEQEAEQQLMETPNKSEWYENSSVEYRATSYNTNESSNLDHHSYKWKSSSFGDDNMIQECGFQFQSSVFDFPFPEDFCANSQNIQTNIQNIENKNQHTPKKKRLYCNNKKVPHWAENLERVSQHQNQQQNLSQNQIFGRMKQRVLDIAKQFSLNRFNKRGSSAQWHISNEKYQQMQKQMMNLQQIQDQNIIKLKQHQEDSVKKNQGLYAKTQYFLTSIKKKILNSFEKEYKFKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JRX5</accession>
    <name>A0A8S1JRX5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0080519</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0080520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JRX5"/>
    <dbReference type="OMA" id="YLMKFHE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="244" mass="28258" checksum="967BA01E0E670552" modified="2022-10-12" version="1">MKKQLPKSASQTAIQKPEKSQTIKTPERHGIPRNVNYYSHFLAEPYITEKDIEFVLQLRNIDHAEMTAKLARIPNQTFSRGDQKIESQKDKHLSRDVKYNGNSTPVLHLLKGRIGPTPHLSQAEFETGLRSYAKTDKSLLEKERNWTTVPKTQRKDAFPEFLPNYKEEIQKKKSMSMSGGKLAKLTYSAFDSDSNYPSYLMKFHDKNLQNVKHMFVPAIKMSTVQWQDGLRPLEPIKKEKKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTK0</accession>
    <name>A0A8S1JTK0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-dependent kinases regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0050229</name>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0140295</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the CKS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTK0"/>
    <dbReference type="OMA" id="ACCWELL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.170.10:FF:000001">
        <property type="entry name" value="Cyclin-dependent kinases regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000789">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01111">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01084">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00944">
        <property type="entry name" value="CKS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00945">
        <property type="entry name" value="CKS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU311113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8043576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8050048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="92" mass="11075" checksum="AEC94F0089C1E1D8" modified="2022-10-12" version="1">MPHYPEEIEYSDKYFDDYYEYRHVILPKHIFKKITKGKLLNEMEWRALGVQQSRGWSHYETHRPEPHILLFRRAKNTDPNTGLPPPGFSAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVF3</accession>
    <name>A0A8S1JVF3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0110130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8046844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVF3"/>
    <dbReference type="OMA" id="FPDEMAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="381" mass="43810" checksum="7789F2DB6CDA357A" modified="2022-10-12" version="1">MSTLAISRCPIVVRHDFPDEMANYIDEKMDEGQYKKRFRYDRPDKKKNKQTKSILNMNSQEIFQKIVMGKKEPKEERFRIKPKQKINTTALHAALLKVKKQKEELDICLAIADKAKQCVNENDICMLANEGAVRFIMSQNLDIDEAALLRTKKGNTFHPKPKKSQSVKQVVMDEPENLLSVEGRSYNMAVSTSLCKHRPTSAFSTMRPVSSTKNYSEKGISEHLLQQYADFRNQLFEKLVDNNENQNEEVQINVGYPQYLTPAVGMKGSRVPPGFLPKGLKRIHSAAATQNIEFRPFINPGEMLFNEQQHISAKRPQTAMISKIQSTSRLNDHLKVIEEVRHQEDLSQLKMSLDQSFREQQQTLNVTGKKRIQSAYPKTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K014</accession>
    <name>A0A8S1K014_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0050432</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K014"/>
    <dbReference type="OMA" id="KMAIHEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14335">
        <property type="entry name" value="UBA_SnRK1_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000740">
        <property type="entry name" value="SNF1-related protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="21"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="460"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="460"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="476"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8043778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="561" mass="64553" checksum="9096BFBF3458F851" modified="2022-10-12" version="1">MTEPANPEKLQNQRTRMIGNYVIGKTLGFGTFGKVKMAIHEQSGEKVAIKILEKDRIVETADVERVQREIHILKLVRHPHIIQLYEIIETPKHIFLVMEMVNGGELFDYIVKNTKLEEVEACKLFQELIAGIEYLHKIRVVHRDLKPENLLLDKSKNLKIVDFGLSNTYKNEELLKTACGSPCYAAPEMIAGQKYQGLRVDLWSSGVILFACLCGYLPFEDQNTSALYKKILSGTYQMPSHLSKDAQSMITGILTVDPEKRFTIDNIHNHSWFNIYRRSYEIPPGIVVGYNRIPIDQDILKQLKSFGIDIDHAQKCLDANKHNDITTFYHLLLKRHLANGGRSTADLNSESFDIKLLEPKQRPNKAPINSLVNNEIIKQQMREEIIKQRSYSTDKDRGRIIKTTEQNNKIIADNDQSVNRVGRNGQASSVQARQKDENYFDQSPNIKKNNRIPTTNILNNIYGTRNKNSNITSTEYRNKDEGKKGSSRNKKELDMTNPHRNTNKEFQQINRILQSGGRSQNHKKERVYVTNQNNQSFDAPQNLNLSAKQKINHIQKFGYYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1E2</accession>
    <name>A0A8S1K1E2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0130218</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1E2"/>
    <dbReference type="OMA" id="CQNIIRF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21742">
        <property type="entry name" value="MobB_NDR_LATS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000570">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000192">
        <property type="entry name" value="Serine/threonine-protein kinase cot-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059233">
        <property type="entry name" value="MobB_NdrA/B/Cbk1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF76">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_135, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="86"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="345"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="431"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8049079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="632" mass="73418" checksum="D5A6DFE59A10A5C6" modified="2022-10-12" version="1">MIGKKEIQISNATRDRVEACRLYIERKYAKQIQEEKQQFEGWQQLSKLMDALHMNPKEQEIIKKEILKKEAEQMRKKRTKLSIEDFQPLAIIGRGAFGEVRLCRHVPSQQIVAVKKMKKHEMIFKNQIGHVTNERKVLEEAKGNNWIVEMKCSFQDEKNLYLVMEYLPGGDLMTLLMKKDILSEAEARFYMAELVQAVASVHKLGFIHRDLKPDNILLDNNGHIKLSDFGLCKDAELHFDKPIFSQKFKSKQTRREKAFSTVGTPDYIAPEVFLQKGYDSSVDWWSVGVILYEMLVGYPPFYTDDPSTTCQNIIRFQQCFAFPEEPKVSQLAKDLISKLVCDANTRLTYDQIIRHPWFGGLSILKIRDMKAPYIPTIRSELDTSNFDKYEEEEPWINKGQKSIKKEMTFVGYTYKQEDFEEKRPIQKALEELELSRPSNSRGNTKTTNSPFQSPNLQFKSQLNKQIPLTQQIKTVSATQSPFTKQQTVSPMPLSKNQNFQKSFLNNRLQTEQNESFHINNQQQQNLITQGNIGNFNNENSNPNSNVQNIKNFIFSKIQQHTKNQNSNTNNNSNIQKTVQFNELQKLVQNQKLRPDKPDQEQTKVISNTNNNISSLKQNIINQFRAVSPLTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2E9</accession>
    <name>A0A8S1K2E9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0110234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000008">
        <property type="protein sequence ID" value="CAD8047050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2E9"/>
    <dbReference type="OMA" id="NPRHIER"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035368">
        <property type="term" value="F:selenocysteine insertion sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040051">
        <property type="entry name" value="SECISBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13284:SF4">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13284">
        <property type="entry name" value="GH01354P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="308"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="242"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8047050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1033" mass="121202" checksum="31101DBDE959F4EC" modified="2022-10-12" version="1">MFAQMFEEHPESPDLIILTISLSQDVPPFYSVQSLNNLTVLLFQAHMIDLAKKVAKFNLKYFEQDIPQLHIISHLNLARIYYFLQDFKKCLSITEKALIHYEPYIFKEIKNSCNPKDIVLLLNGYIFYAKTVQNLQVLGFCQDLDFQQFFINGAKLGRKYLGPSHHITKLFLFSERGSNMKKSINNYIPMKKTYGKILNTQKVCFQVHQLMKTLKKGKAPLFKNPRHIERSISEAQGFKTFKRPDSVKPQSQRHELNQLQKIQPANVQKGSFRIQSPKLIQSQQIQPQNDLEQIIQRKIDTFMKSQSQKKQEPKIVELEKKIDELTKENKRIQNQRKEREIEVEELKDKITQLSAEASKIKFRLQEQETAQEKMKQQQQQQQQQQQQYQFQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYSQVSTQQQQSSDSQAAIMQERMSFGVASNQSQKIQNLSIKKQERQSTIDIHNLINGLVDTDEPKINSISMINDTDFTLDLNLTILDQMDQSKQYSINLTTDQTIVKKALIDNVLYEISCGVVQKNKELSIKISLNTKQEHSTAKIEYVELSIIKDLIQFIDYKNVLPYTQQLKCITTFKQFIQYLIIPFISIKEDEGEQKISIYAQPQSLLNDCEKIQLCDEYCIPLLYPLEDGIFRVLLNTIAIDLQFDQSSLDQFFDVYGQDDYEREEVQEMIRMQDNLEKSNDTVKPKHKVYNIRSYIVKNQQKLTQFLQKLVNDLEELLYRIFNVQKFSQINKILSGTQITMPNKSQAKVQMCLIKIWDKNTFQRIIFIADNSREQCFELFLSNTFETYGPRKSKVKAMGHTQIQYDNVCQKLGLNVSSLDQVDSPLICNILCNCYTLLTFTKELDADDQERFKENAILQVDLSCECNCRMFLTNQNSMYKIPLTMSYLGIQDKPIGIRVQIYDVHTNTEQGMFLSISKETWDKLEIQVKGKHSRKYTLQNKMSSEYHLPQILQNGGFQVIKEKLTVEESKTYRIIHGKHISFVDRLENVLNSYSLLEYLNKKLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2M0</accession>
    <name>A0A8S1K2M0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA ligase family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0140015</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2M0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07896">
        <property type="entry name" value="Adenylation_kDNA_ligase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08041">
        <property type="entry name" value="OBF_kDNA_ligase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029319">
        <property type="entry name" value="DNA_ligase_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050326">
        <property type="entry name" value="NAD_dep_DNA_ligaseB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006592">
        <property type="entry name" value="PRK09125.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47810">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47810:SF1">
        <property type="entry name" value="DNA LIGASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14743">
        <property type="entry name" value="DNA_ligase_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="5">
        <location>
            <begin position="280"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8049489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="441" mass="51115" checksum="F23578968B65087C" modified="2022-10-12" version="1">MNSEIYLVKLNQLFWHSKLLSTNPVTLQIRSGNLNTNWEKSENLQIELKKYNSLQEGILDIGEMLKLKLRQGYHEIKSSEIVKIYEQINFPEAEYMYELDLKYKQFQQQKGLIDDKQLTKKEQIVQKIERKLKQPGLNYPEKSCSELLDDATQGKLVSVWPKNKMRPNLGENLGLGPTFSTSQRISCLLAQNWTVSVDPKGYYMSEKLDGMRMIWTGMEMFTRNGYSLKFPSYFIEGWPTTYLDGELWINRGTFNKVLTSVRKRTPDPNAWKTIKYMVFDAPFLQEPFSDRYAKLKKAIEKIKNPHLIYLPHKICRGKEHLAEELAEVQNNGGEGLMLRDPDSLYEGIRSKSLLKVKSSLDAEAFIIDYIEDTHMKKQGILGGFLVRNDQGLEFEVGLGLKIGIKKQRPSIGTKITYKYCGLAENGYPKFPEFVRQYPQDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4F9</accession>
    <name>A0A8S1K4F9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0140446</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4F9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035877718" evidence="2">
        <location>
            <begin position="20"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="296"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8050340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="387" mass="43341" checksum="A0E6B0C19DC8F786" modified="2022-10-12" version="1">MKTIILALALIVLASSTQADVIATIKKIDQSPFGRTLFDTIWLELQTGDPLDRLLQTLTDLEDRYVAEQKEDDARNHEYQDACTVDISAFDKDLAESNRKKIELEARLEGQLYPQRGILQGLVAQKQAEVKGYQKDLDELDAQRAEENADFEEKVLEHQEATAIIAEARRLFADNIEHESFIQKGKATRQPVHKFTKEVASMIQKHFTQSAKKAAKFQHRKGYSKLFKAFATIASKVEQLADAGAVSKIIDLADELLAKIADSLSLLRFAEDKRVEAYKKSRNFVVISLTVAGTALANAQSDLAALNDVIAQVEASLDTTNQRIENVTADRTDRFTQCEEAVQDYQDARSARTSDRDVVSQTIGLVNKELRTLREQLALRQSAGEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8M8</accession>
    <name>A0A8S1K8M8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0170391</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8051101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8M8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8051101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="768" mass="90271" checksum="BF726BB888DB815A" modified="2022-10-12" version="1">MKKIILQPPSKQYQFQQNQLKQHNKNPQNFIEEKKIFELQGKLAYYDPYKYSQFFGIVQVQIEYSKNGIQTIIYNGQILRKQKIYGTDNQHQNIEHLEQIQYLSWEGNYGKNNQKIGKWIAFWKGERLLAGGNYDEFEYKIGQWVELDQNFCEFIFIFINLYQLLSSFPFRKLQKWKKISQLVVDYMMRMNKEMEVGLIYISISMSFQLFSIQSWNQITYSGVYINDKKQGLWNTIFQREIIGGGQYDENGLKNDKWTDIDDQICDMYNNVIYVGEYANGIKKGNWITIKNGNIIGGGNFDVYGMKNGKWIDVINNFNQRNNITEAGSYLNGIRVGQWNYVYRGQIIGGGSYNDEGFKNGEWIQLYDNSKRYIDVLCKGVYQNGIKSGKWITINKGEKIGEEQYNENGLKIGNWIELDQQFREYKQVIYVGKYLNGKKHGQWDIMRFGNIIGGGQYNDDGLKIGKWQDLDDSFCNIKQVTYVGEYQNGVKYGKWETICFKIIIGQGEYNYIGQKIGKWIDLDDHYHKQEFILINSLVQNRQIKQEYIKMGKNMGNGKLIIKDKSCGGQYDFDWAKKGKWIEPIQNFYSQKEILFEGIYNQGQKFGKWKSYKKSQTLGGGLYNEQGLKHGKWIDLDDDYQSFRQITYSGEYFNGKQKGNWYILYKEDLIGGGSYNEQGLKDGKRIEIQDNFQKFCQLTYAGEFQNGKKKGRWFILYQGNAFGGGHYNQLGLKNGEWIEADDSFFDWNMKIQFITYHQGIKQNQQLISRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAH1</accession>
    <name>A0A8S1KAH1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0190033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAH1"/>
    <dbReference type="OMA" id="KATWDIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="44964" checksum="64E9AF5CFA7BF444" modified="2022-10-12" version="1">MIILLILIIQTQGRNLQAGSPQKQGDKWDQSNQALDQIFEHLANNLTQQQEEELELIEGYIFNEGVLYYNQATQEEKQKIFLSLIGMVGELEKENEQEHSKLEKDIQYFSQLNITDKNIVMTKISQNLDKTLIDSSKEFNSKVVLQIIKKEIENFQSNPDRSTNATWDILEYKMIQEKNEQELMEIEYQIQEFLDQGLSIEKIREKLTEFIDYFFANSTLLDDNNNSIDEIINDVKQQEEQSQPTQEMKQYRQKVRELIRELLKQGKTEEEIQKEVDDYLSKNGVNDLSDEERNLIKLIIQKEIMRNKRQQDYLNDDETKITFKNQNNMYYIYLGIFTLILLISLVVFLIRRQKMKQNQRKQIGFRIQDNAQDVEQME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KGY0</accession>
    <name>A0A8S1KGY0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210198</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KGY0"/>
    <dbReference type="OMA" id="YILYCSA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="273" mass="33105" checksum="65B038441226F11C" modified="2022-10-12" version="1">MLQLIQQQIQEHTQAKEKKIVQYLDQNQFSLYEDLLILEKMLNEKLTYQHFEQLSEKCHKKTPDELKLRYINCIKKLTEDDLKQIIEFIKNNGLEGTLIYDKNEIKICKINEEIKKKKDSIQNKKEIKQLGEPLLKKKRRIQPEVQPQPQNYYQLTDLQKYILYCSAPVYQEQRFPTNPNYQQSQRLRQEQFQNSLHDLSLWYDIPFEKMISLLKACSGSFNNLKQFLEDKNYFIYWKPEEDQKLKSQEEIQSLYLTKGVTEVERRKKWLQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHN6</accession>
    <name>A0A8S1KHN6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0140460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHN6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="74" mass="8933" checksum="A187BC5550669036" modified="2022-10-12" version="1">MMKIGLIDYFSSIQYFSYAEIQNLKSTNRSSQFIDQFKNFNVDILCLQEVDNLKFYQEPKDKLNYDICYLKRPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KHT3</accession>
    <name>A0A8S1KHT3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0210153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KHT3"/>
    <dbReference type="OMA" id="HEFAETM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035756004" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="205"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8053871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="42072" checksum="1285690D73D5BBFC" modified="2022-10-12" version="1">MKTLFLLLVIGVMCQNEVDTVEKLLADLKGTSAVELTALNTDYTVSKNLKENIIFNLGKAYQEQNFLCASRDKSVSDRESDIDKTNKYIAYLQKRLVDNSNRIQTLDANRCTHSSNYIERVKNDNMTLRLIIFLRNSLQNLDNNQLNRYGSYVEKFLTMYKSAKISELVELAHEFADTMFIDWNQVTPSSQGNLNTVKGQLIEMLNDMERYIREQIKNVQNLEIATGVTLADFKGAIDIENEQINYDLSSEQNNLIKLEEQLVQARLAAVNCREKGVALQQQQQRAIEDLKNEEIQYNKNKARLQEELNLFTDVYRIYSTQVGTSEDKFKQRVDDYVNDRKVDSYERSQYTLSDAVRKQVQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KI97</accession>
    <name>A0A8S1KI97_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0230064</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KI97"/>
    <dbReference type="OMA" id="CKSQTVF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="105"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="143"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="105"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="143"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8055210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="234" mass="27448" checksum="FB020D88C473A5A5" modified="2022-10-12" version="1">MSDVHKIRTYKQRTSVQNKSQKINSERRSLNTDLNNYIPSQPSWTQKCLKNAKRFSFNDQPDLDKRGSMSTVATLASMNTDFYVQDDDPIEEMTSSSDESQSNTKFKTEMCKNWSLLGKCNYSNKCQFAHGENEKISRQSNIKYKSKLCRSFHQEYVCFYGARCQFIHESRSIEQIKKDCKSQTVFYQPSNYQLRLKSFQSITSNWQQQIPLQECLIQWNKKLLIQINLSSSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJP6</accession>
    <name>A0A8S1KJP6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0220027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJP6"/>
    <dbReference type="OMA" id="PMASEMR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="463"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8054433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="66303" checksum="B9CD3C255C2D2EC9" modified="2022-10-12" version="1">MFFIFLQLFILTNSELIQILTRQEAVLDVFSQSGIILEQQLTEQQLCKIIPQQLVANNIDDNSITLIDSYSDDNFSLDFEYGSYKGKIGKILKFVQIKRGILVLRDDGELYFLRIQDTKFKQQATFKLNIDEIALQKTVFIEYFYEQNSVLIIGDGETIALDLEFDDDDLYIKQYKVYEQWQIQHINSIATANNLIIVAMNTGIKMFEFANHQLSEIFLDSQMVGKIQDIKVFNQNKDDNYFIYLLDEQYGVSQYIYNIGRSTILQFNSHMGQIPYPGEILDVYGDILMIVKNQTLFEFRIDYYKNNYELIKQHNLETDIIDIQLTDTFAIIIGRNGHQILFHSIPSTFSQFEWMNQLVIPNLKQLDILSLILDIKSHKNNNKTTHNIIVGITQHKFFFSKIELQQSYIECYSDSTTSELELIYQQKSTYCKSNMKNKICQLNKTYTIQFVDPKAFGGGKDTYLIIVLLYVISIASFLVLMGTLIILYKKYHQIHLPQQQQLDEKEEQPYTSTTRHIKMASSKKIIYLPKADQTPISGVQEQINLQLNNNNRPITQDNQDLSPMASEMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKB2</accession>
    <name>A0A8S1KKB2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0220229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKB2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="100" mass="12057" checksum="0FFB5CBD97504811" modified="2022-10-12" version="1">MQNPNIYLEMNLLQKKIRERIQKSSQQQIDEFSNIVIIVLKPDYEYEFNFSISQFSCFNTIKQLTECVAGQFYNQTNNYLNEPFYSCLLVRLKLKDWIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKZ0</accession>
    <name>A0A8S1KKZ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0220235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKZ0"/>
    <dbReference type="OMA" id="KWRRAPQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8054843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="821" mass="98797" checksum="E947BE43AFAA524B" modified="2022-10-12" version="1">MDCKEHCATFLKFKIQEQKPNRNITDQNQSISQIKVIKQHFLNQQNEINNIEQLQKATSNLYYAMYQIKEKKNIYMIPFTQLKFDKSDWLIMAIINLIEEKNKIIDELGETEKNKYENAIKWTKKIIDSYEEFDDFYIQILEVSKQLKKPQLNEQFSNLLVLQQNLNVNKFLKTFCFIQNIKLDLNQYPEVSQFSQEIDEKMNQNQYQFDNSLKRNLYFNFTSDIEQIDIDFWTFYMSDYKETNKLIILYPIIQNKQEKQLKNNTRNELIIPIIYNNSVQEYQMKLIYKSQLEQQIKNFKPDYIYFEYQISDQFTLDLNALEYLIRQLQKSAKFIIHFRISQNQDFSEYQLSTNLNAIVMGLQGLKYSEKKFISENQFKEMGIWTTEMMRLKENSNKNINLSLQSELQLIQIQIQNIQNKIFGFKQERNEFIWIQEDNININTQHFVYKDTIILYNKLNRDIYYYSLKPSVKNIQNLPQKIEFTKINLGNQQNPVEPSIIKIQNYLIFAYGHNKNNNNNDLEFSDKIWLYDLQNNQQLPIIISRDEEYDEFRLPYYNKVRNSQDIVNRIKWRRAPQICKNIMFNDDNQNFLSFILIGGETLKQKQVQQSNSNSIMNIIEVVILNLKNQKFCSFIVNKSLLSRLSSLKPWPYQTVLDAKLENTYFYLVLNGNQTTKQDYYSFPKNPQYLKQVQLLVQSEKTFQLFILSLKPSQDPIFQLDELVDDCKLQYYVEQKKENNCFVWKLIITRFVLISNQLINHRLFRPLEDKLKKMQKQSIHFCQICFQVNVKLNLQVQEQSTQSYQESYVEIDYEKIELLQNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQI8</accession>
    <name>A0A8S1KQI8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0230196</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQI8"/>
    <dbReference type="OMA" id="DHQNSWF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="5">
        <location>
            <begin position="147"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8055472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="272" mass="32192" checksum="8EBA4B08B7DFE3B3" modified="2022-10-12" version="1">MKSQFSSQPKLEQDAQQQPYSLPPLPNQDRIQYYPIFYIKQDDQPIQSNLQQILTDHQNSWFVCHPVMPNDPFFPYISKFSNFIHKPYTQNPEIPIKIEDDQEFKQTNIRQQFQKPHLNQNQEKQQNKKQKDQNKFKRNVDNNKELEIQPCNCSQSNCLKRYCTCFHSGRTCLDECQCKDCKNCAEFAEIRDEAINHIYKKCHRDKKVPATELLSLQMSYGCKCKTTGCQKKYCECFKRGQICGEQCSCEDCLNIPFNLCQKDLNRKKTVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSP1</accession>
    <name>A0A8S1KSP1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230305</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSP1"/>
    <dbReference type="OMA" id="TMKHIAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="427" mass="52465" checksum="636708C96869993B" modified="2022-10-12" version="1">MFFIFLFFIQNSQPEHIIIHYPQFCAQYRFTFIKVANQLNLSILNIPSEEQSIILLNNSNSIIQYQWDVFELDIKYWLNIYFYQQFPILNMPVNQLNQIQENLLIFYGLNQSYYTYLLNLTYDYPLYMFYYANPHNHKINQEFFIQNYSIYQDYLIIRRRQDNYDHLLLIQSLNQIKPFLEKYSKPYQQQYFTMKHIAYLKQNQNSRAFLYISFNLYQYKEQKLIIQRQSDIYRQSKSNKNVVFIITEPIFMAKILLQRFLYKNIKDGPFLIYLEFNQFKFNIFNKEWNNYYVSQWLQQYIRNEFQSLLFNLNQYLQQIPYLTLSQQKVQTEIQNKIHSITLLDLILQKPLQQNILLIRLIPYCYNSFKLFSKLSKFRKDNLIILYSINQLIYDEYEMLMITNNQTLIIDNTNDNQLIQTLIQYIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTB5</accession>
    <name>A0A8S1KTB5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0270063</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTB5"/>
    <dbReference type="OMA" id="ESNKNGM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8058137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="44469" checksum="590A371F82479B1B" modified="2022-10-12" version="1">MGSNCSNCGQCQKDKQELLYEIDITKKTESNKNGMSMMSQPSQASNHSVKTQTYKNQVKDNKELQYQKKQEFLRSQMAITIQKQWKGYKARQIFKKLKQSLNDVKKYDEQETSQSKLKRNTFKYFNSEECADINPNNTCREIRQKYQFKSGAIYEGEWLGNKRDGQGTQIWPDGARYEGQWKNNKANGKGTFYHADGDSYSGQWENDKANGYGVYKQLNGAIYEGYWQNDFQHGQGYEKWIDNSSYKGEYFQGKKQGKGLYIWPDGSIFNGMWFDNKINGYGEYIWADGRKYKGNWKHNKMHGYGVYQWADGRAYHGEYQEDKKHGKGKYFWPDGRMFEGEWIDGKQNGKGKYILADGQIKYGLWECGKRIKWEDRYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KU23</accession>
    <name>A0A8S1KU23_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Insertion element IS150 protein InsJ-like helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KU23"/>
    <dbReference type="OMA" id="CYNYRNI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055247">
        <property type="entry name" value="InsJ-like_HTH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13518">
        <property type="entry name" value="HTH_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Insertion element IS150 protein InsJ-like helix-turn-helix" evidence="1">
        <location>
            <begin position="11"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="139" mass="16694" checksum="B9E237F4D4981B84" modified="2022-10-12" version="1">MKYAKISVDTRKAFIEKVKQGACTIKQAAKQFGIKFSTGKAILSLYKHEGRVGKKERRNRRFKKQIEERENQTSKCLKDEPPVEAKQVQEVQNCQNNQSYFNYTQPQYYNYQQWYQTQAWLQYMNGLSQMNCYNYRNIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KW45</accession>
    <name>A0A8S1KW45_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0280200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KW45"/>
    <dbReference type="OMA" id="NNNDWKS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="278" mass="31801" checksum="83C302C11F387DD4" modified="2022-10-12" version="1">MENQLPIKKKTYHSESQQDDKNKSGSQKIQTDKLFASLQKITLLDILDEFAKESENKSITEILKAILNNVENKQKEPESKVDWNTGKYDKPNNNYYQKFESNNNFQNNKFKKRNTEGGWNDNQKNDYGNKENGRGGFDKNGGNDRTKPWENKDGRKGNFQRGDNWKYNQEGRKNGGGNGDWNNKQGNGNNNNDWKSGGNNNNDWKSSGNNNNDWKSGGNNNNEWKTSGNNNDWKSGANNNEWKSGANNNDWKSSSNNNEWKVEATTEQAQPISWGSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L267</accession>
    <name>A0A8S1L267_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0300038</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L267"/>
    <dbReference type="OMA" id="WAPIYLA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER 16, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184:SF12">
        <property type="entry name" value="SUGAR PHOSPHATE EXCHANGER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8060245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="445" mass="49874" checksum="67CF78D55798EA2C" modified="2022-10-12" version="1">MLIKFKRIFVFTLAFIQYVFLHSCRSTWSYVSGRMAQPGPDNYFNSQYLGYINFAFLMSYGLSVSAIGQLGDRMNQKFFVLIGAFSTCLIFCSIGFFLHYEYKHPYLFMILQILNGISQSTEWSGLLAILNNWYMGPNKVLILGYFSASPCIGNIIGDLYSGSLIGKHDLPYYAPIYMSGLSLFTISIINLFALQQAPFPNETEELVEHQESMSISLEPILHISKENSSDSGEYSLPSSHFYKESEQLSLSYYDAWFVPNVAMYAFAFGCIKSVYYILGFWLPEYLNKNKVPDVAWITAMTDIGAAPGGIIICLIGYYTNKRAIIQVPSLWIGTVIMIIINYADNLTIFGYLILVFIIGFLIGGTYNNIAALITVELSNQDQLKNNKKATATIVSLIIGYASAFSAINQLIVPLVNDALFLYCGIMSIIGGIVLSPLLIKEIKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L8S5</accession>
    <name>A0A8S1L8S5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0350105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L8S5"/>
    <dbReference type="OMA" id="PKRICLN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8063719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="120" mass="14081" checksum="455225977AF88EEA" modified="2022-10-12" version="1">MGNATTKLNRKFDGWLKKAYNFIFKEEELSEVSDEALSQEEENENAQELQENNEPHNQQEQEQNQEQEENFSTNSQEMESIICKSITKEELIQDKRKASTLLSSEFEPKRVCLNKESMTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9T5</accession>
    <name>A0A8S1L9T5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0320044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9T5"/>
    <dbReference type="OMA" id="YPNIDYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="332" mass="40273" checksum="E68696EF4F370ED5" modified="2022-10-12" version="1">MNKVELPSNLQISNFLQNEIQKTDKFVKQHEKQLNTLSQYQKIITQNSQSNKEKLSQLFSKQFEVDQQIKQKNNQLKKKRYINEQSYLQQQKEKNYLQQLDSYWSYQQNFYPNIDYMSDSVDKSRQQLIQYYEHKLSLEENQLERNQIKQKIFSLREAQNQYHKCKSIEDKIKKAEQLRIERFDTSVDKIKKHEQHILDVIYQNQILSERKSHYFKDKLQRLEEKLCQSELEQGKKRLDQVQKAFQKDNHRLQVKVNQEKINQQNDSIHISHLENKLNKINSLEEIRSIIKKGRLQIANNMQQQKNEIIQQIDDQRNSSLKSSIYQSQLLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LAB0</accession>
    <name>A0A8S1LAB0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0360121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LAB0"/>
    <dbReference type="OMA" id="PFQEDEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="185" mass="21546" checksum="E671402DD0213429" modified="2022-10-12" version="1">MLIVQVRTPKIKMSTHRAQNSQPIMFNQSNLNSFHQFFQNQSRSLQKDEGTNNSRSKTELIQIELKANHAHKISSLSTTISLTNSTSYNQRLSKKNQKSNSSNKIIEKILPKSVNPFQEDECTVNQKLQSQLQKLLIKTKDIINSYEINSKKQQITEQQLKQQIVQLKKIIENQQTQLKKHNIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LGZ2</accession>
    <name>A0A8S1LGZ2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0380056</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LGZ2"/>
    <dbReference type="OMA" id="FPYKGST"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001337">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="93"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8065751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="341" mass="39165" checksum="D9BC4332611F267B" modified="2022-10-12" version="1">MQQRRPKLKSLDNKENHHTSETPKQTRKLFRKTTPKLLQPLQMDQHISSREPLTTKVDGPHKIRLRTYVNTIENLMNDNLLKLIPPTCDGTTYSIGQSIGKGSYATVRLGTNKQGFKVAIKVYEKLFLKGERLNNLVKEISALKALNHEQIVKLLDVYHCGSTINLVLEYCGNESLFTLVKQHRALSKDYAFNIIHQLLKILVYIHEKNICHRDIKLENILINNKKIKLIDFGFSTIYNCITCHCGTPSYMSPEVVTKQKYDGRTTDIWALGVLFVGLLQGSFPYKGSTEKELFSKIRNNDYTINSQDKDVKVFLGQIFVLDPHHRATAKELLQCDVFRSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LH70</accession>
    <name>A0A8S1LH70_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0370325</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="PRMINDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF360">
        <property type="entry name" value="ULTRAVIOLET-B RECEPTOR UVR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="293"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="318"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="369"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="423"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="475"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="707"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="761"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="813"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="235"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1066"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1066"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1076"/>
            <end position="1094"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8065545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2041" mass="235813" checksum="5065C6C33F6876EA" modified="2022-10-12" version="1">MADEPDDFKPALKTISASINTTMAISEDGDLYVWGKTFLKNKNVKHLPTKLGLPNQYLFYRVVHISQGLNHGAAILQSCLDKECEGVITNQKGQVQTILGMVADQAKKLKENSEQQQQSEYFLQQWDQPFKEFFKSKNNKTILSLQEFRQFYRQDKHLKNISYQNISEVKTILGTGDEANKDEPNAFEKFFMMEKSKDGKTEINICYYYQMLTQESRPEYISIVWGNPANGRLGAPKSKFPNETDKQTIFNNNNPSQMDFTSRQMQQPSRTQQVQNSQYNQTQAEPNNSDFIQPIALKQEFVKIVCGHHHTMALTEEGSVFIWGMGSQGCLGNGRVDDLETPTQIDISGLYFNEIAAGAYHSLALAKNNQVYGWGVNNRGQLGIGNKENQLLPKQLTFFDRKKVQCQYLYCGDQHSACITTNKELYTWGNGDYYRLGHGMLLDEMEPKKIEILQDVYVLDVALGTIHSLCITNEGFVYAWGSSKDSVLGMPTQQYKDQTLPQRVGLMSQSFRDASFYQVAAGTSHSLALSENGHLYVWGSNKNSLLGVRLQGQEHTCIPQKVEQSEDLEKSCIEKALFFNLKKEEQQMMKDEKLVTFEQTTLMKTILQPSQGQSHECKYKYATLGCKNTYFIDNTGHLYACGSNQKGMLCKNPHKKQKFKQFIPQKDPPDILYEQSFTPIEIVYFRNTIIRYISCQYNHCIAVTAKGNAYCWGSNQYNQLGLGFQSDHVFTPILLEGAIEQKVIIMAITGEKFSALLTDQGEVWSFGTSEFGVLGHELKNFYVIMEPRMINDIPSMINLACSNQTMFAIDQKQQLWAWGNNQFGQLGISLQRDEEEINLQTNKQVSSEFLEWKAQLKSLKNPERYIVYQPDKPSTRSITGEAFVDQVSCGLYHTCIITTNQELLTCGLAKYSGHQSQEPEDPEQQQAQQAQFIDAFSSTLEHSSKKFKLVACHEYHTVAVSVSNDLVFLGPEGKERGLHEKFANLFRDGKQMPEELQNIKFVACGPNHALILNDQGQTYTWGATMGGRLGLHINEIGLLDGQEKLTIPYPTKIECEVCKDNKLQAKNNKDKDKSSAPKKKEKKKNKSSKAKKKVPGGDQQLDVSIEDEEILDEESFHSEEGPGVRVQDPLQKGLSKQSQECEQENLYLQDAEIEKKVQFIFKDSAIQAYELQKMYTEIVSKLSWSIDYRARQLAAKNQSRQITNLKFSVPQFIVKNIQLYECIFSCMYHHPCYMIKLIQANQLNKFQLLKFIKVIFKNDLMEAQNQSIILTLAIKSFEIEFNNATNQLEYISELKLFKSSSVFLQIYYMFIQKDEDAFKYFDEVANLLIKGFAFSKILEEIHNFEKRPEEAESDDHKLKNFFNFHQENANSKEIQAKYDVLTELIIFYIKSIVDRKEKCRIKGDEMISSHIKYLHNQIVQIMKKKILKEELVKITEVVEDEAKVQQKLQKFQVKLDEKIGSFLLHLYYGSITEFILKFQDQIQSMPEEIRPFLLNDQNLRQIAQTIDRFFQKDVMLPNKYNQNLNKIISSRMLPKINLLGIQDVAQDLQGFVQFSVENLNMIELIQECFEIKDRYMQVEIQDILFLQHFYCSQILNNGLQAVSKFFARGMKDPIYIIFRHLEFREIQVNMFSTEILKRKINLKLMPTPIMQLIRKSNEIGLYKCEDCSILLTTEYITAEKLKSLQNLFANGWSCAAGHHNNITVLDCESPGCSNFITDQALKTMRVLRFYYREKDDNIDMLVEILSSIPTIPKGINMYNFLKDLQKTNWVKRNSRLTTKIEQFLSKIFPSELTDPFIRSETEDAEQALQQVTEEQKEAWAVLAAQKKVQREEALHKQYKIVYEKAIIQMNERSQHRALQITYNDLIRQLELTRSEMKSDLHKEIKMIKQYQDFIQQNIRVYGEGEVQQPERAKGQAYGFYTSANMFQAKALVKLTFQNRQIPKILKNIKFYFFGQSDGGWDIQLVYLQDSLLNLLVTCIEAIYPIELSLYKISIPIDQYLELRRMAKYRTQITFKSKNIEITFNIVQLISIFNQLEARLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LL51</accession>
    <name>A0A8S1LL51_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LL51"/>
    <dbReference type="OMA" id="SFRYNDM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="252" mass="29734" checksum="DE90B7ECB0EB0DEA" modified="2022-10-12" version="1">MNNQDQSILVDEEFNDKNLFSHYQKTRRTNAKFNPNINLQDYQINTINRYHIGYLEHSHIKSTQNITINKLQLQKYNLQKPLSKELEKPKELLNRNSTVLDLQSKNIFLQRQSGIGKLKPIMQKEILVNPISKTQRLITKKTNETPVIEVCNSNYTEQKPASFRYNDMQLSQYLEEFKLLKQETLTTVIQPHVSCKINRNTNNYTQTSNRQQTTNHYYHSIIPKSKVKQRLTNIESQKQNLTLSGWSDYSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSJ8</accession>
    <name>A0A8S1LSJ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0410113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSJ8"/>
    <dbReference type="OMA" id="MMQIGKI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8067686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="458" mass="54418" checksum="8D7D051666CCA555" modified="2022-10-12" version="1">MLDFEQDFYKFVKAQLDKKRGIKKLASNDQHYHEEIIQRNRLQSENIQKVLQNERKKMKINKVTKEQHFENKMRDFSSMDTFIDVKFFLKNLNDQQMKKNRKKLPKLIKRSKSYSNNEDLFDYAEIVQKSKKFIANSRHFRSKPQIEVYLQQTDNEFTQRSKVIEDGRSTNEKFYTGIDQLITDINHNNALNEMDKKYEKERKKNRDSMNSEQLIQSLIPLDFGKDEIAIEAGKNFKQQSQKVLQLLERTTKMLYKNKIPQPPNQKILKQQQQQLKELKQQLPNSIEPKSQKQILVKSRSESNDLKFPQLNGKLINPLVRRVIPLQQTSHATLPEIQPGSFLSLRDDKKFENLHYFKSPQNQSKQDDHHNNSSHQSISLNLKNQFGEFLTQLDDAQDQFQKQYPTDSQLQKIQNNMSKKMMQIGKIPLESFKYLTTRKFNVSKKYELRGKKNKALRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUG9</accession>
    <name>A0A8S1LUG9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUG9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="34" mass="3817" checksum="B255893DAEBD9394" modified="2022-10-12" version="1">MGPGLDDNLPEIESNLIIIHLLTVERPRTYNLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LUK1</accession>
    <name>A0A8S1LUK1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0430298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LUK1"/>
    <dbReference type="OMA" id="LELEMWN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="143" mass="16530" checksum="82E871DB33793A54" modified="2022-10-12" version="1">MYISGYNNGATIPSGYVTLKNCSTAIVYLSIINCLSKFDFNCPVFLTVLFILLYLSNSINQHENKILRYVFYLTVVTIVLDAIWFIANIKIKMKKYSNLEVNGSLELEMWNSQFGLHIMELILSSLICLFKIPFIYQCWQLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LWM1</accession>
    <name>A0A8S1LWM1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0450001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8069991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NIRCQER"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8069991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="183" mass="21410" checksum="620AD3E6B0FD3E81" modified="2022-10-12" version="1" fragment="single">MNCTYHIQNPITSICIAPHKCQCQRKLCGECLYDHGVDIQTQTTPINKFRDMALKKIQDSKLDDNSELTKQRMAFKSLISQTDQKLKKIWEELSQSIQQVYDWIDKENQSYLNLIKENTNLAESSYTDIEKLVNIVEGTTLIDWNASKNSYMKELDKTKCWWDQHIKAFIEKSNQGIQQIQSX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXG9</accession>
    <name>A0A8S1LXG9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NUC153 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0510084</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXG9"/>
    <dbReference type="OMA" id="DHDFAID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039754">
        <property type="entry name" value="Esf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012580">
        <property type="entry name" value="NUC153"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056750">
        <property type="entry name" value="RRM_ESF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202:SF0">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08159">
        <property type="entry name" value="NUC153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25121">
        <property type="entry name" value="RRM_ESF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ESF1 RRM" evidence="8">
        <location>
            <begin position="144"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="NUC153" evidence="7">
        <location>
            <begin position="498"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="59"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="507"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="64"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="104"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="515"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8073498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="63308" checksum="D1CAD2FA3EC332EF" modified="2022-10-12" version="1">MDDPRFARIKKDPRFKSLPKKQLKTKLNKKFSSILKDPKFSTKPKFDIYGNKRKYNSEFKDHYELEDEEEEQVIQQEEDEGNQNEDQSLEQDQQEDSKEKSQDESDQNEEELNSEEDISISSGVDNWEDEDLDQKNAPIILQATKRLALQNYDWQQIKAKDLFVLFSSFAPTGSSIQQVQVYVSEYGKQKLAEENEFGPRQIFKENYVPKKQKDEVSAVNELIVKGHEEDLDVDPIKLRQYEKDRLKYYYAVIECDSNKTADQIYQQINGKEFELTNIKIDLRFIPDDVVLPKDNLKQECNTLPSHITSNNIINRAVGHTDVKLTWEDSKPRTNFWNKNIKNFNKEDFEDIVGNYESEDEEKIKEDVKDLLNQVQENKNDEEEDWRNSFKKKSKSNLKITFQSGFNPDGQNSIIERMGQKSKKIHVPKEGRIDDFFVGFQEQEQDDDDNEGRPKEHLKYLERKKLKKQELKQKRFEQAQLKLLVEDDKKPKIGFNPNDKRFEAIHTKPEYAIDPTNKQFKSVQNEQEQRKKQKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXJ6</accession>
    <name>A0A8S1LXJ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXJ6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894:SF5">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE RSMI, CONSERVED SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8070695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="338" mass="41229" checksum="87F263EEEFEC589A" modified="2022-10-12" version="1">MMMRFIKLIHRKENQEMRVMFHLVKLNQISNKANQNQQQKRNQEYLLVQQNQLLMLLIYQNKRTGLYQQSNILQNYQELIMKIPNTIQNTPLHVHMYENIVAHYGQSQYSDAYRYMRTIQSFLFFKQKAVLVNQWLSFYKAYCQVFLNMISRILIYIYKQFNHYKNFQVFHTQNSLIHYSNGFLILIIRKIIHIILFKIQGFFNSYSFSIKFIFNLYSYCNKYSQSEIDNIIYDISTSEVSYMQSQQGPFDYDILMYQFLEIFKKYKIRTSIKYNYLYTSIDLINKGIVDFQQWNFQYEVLCCINYSFSIRLFYQEADYCVDNGRMISKQRFTLVCDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LXW9</accession>
    <name>A0A8S1LXW9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0510170</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LXW9"/>
    <dbReference type="OMA" id="AKFYKMM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001033">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="10"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8073648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="294" mass="34015" checksum="AD73EAE523986B90" modified="2022-10-12" version="1">MQVGLNKFGFSFDKKIGSGSFGQIFLGINKQTGQDVAIKLEAIANKHPQLIFEGKLYKVLQGGIGIPQAYWVGSEGEYNILVMELLGPNLEDLFNQCKRHFSLKTVLMIAQQMLSRIEFIHSKNLIHRDIKPDNFLIGLAQKNDLIYIIDFGLSKKYRDQRTNLHIPYREGKSLTGTARYASVNTHLGVEQSRRDDLEAIGYVLVYFLNGQLPWQGLKTDNKKDKYEKIAESKIATSIEKLCEGLPEEFSIYFNYCKSLKFEERPDYVWLKKLFKDLYTRMKYPNDNVFDWTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZ51</accession>
    <name>A0A8S1LZ51_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0460170</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZ51"/>
    <dbReference type="OMA" id="KIPMLCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034338">
        <property type="term" value="F:short-chain carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000667">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000952">
        <property type="entry name" value="AB_hydrolase_4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF63">
        <property type="entry name" value="ALPHA_BETA HYDROLASE 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01133">
        <property type="entry name" value="UPF0017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="181"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8070851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="402" mass="45960" checksum="D1810EF1F855CFEF" modified="2022-10-12" version="1">MKVGIIGGISSYLSYLVWNNYNSNQIELYSLNTELQNEIIQNCPSLTSYRPPAFYCGFLHTLIPTISKPKEKHISFFEKIGDTGISVDFVDKGQSINSDQPLLLIIPGLTGKVQNGYVNSLITQAHQYGFNNICIYTYRMLSKEANFLFVPPFQDYGIDFQGADKEYFRHDVVAGQTTRVDLAADLHYTLKYLKDKYKFKQILAIGCSYGGVQLGNYLGRFQDDSLIDAGVTVCSPHNITDCEKNISTIMDLTMTYILRRGLKINKHLFYDPNNYPKQWKPQISKAMNSLFVHQFDRYYTSQVYGYKSEVEYYKHFSSCDRFPQIKIPMLCIAAKDDYAVNIKALSLDKLISNKQTIVCLAKSGGHIGFLEGLNADSTWFPKPALEFLQYFSKEQEIEYKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZI7</accession>
    <name>A0A8S1LZI7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-galactosidase</fullName>
            <ecNumber evidence="3">3.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0500113</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.22" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZI7"/>
    <dbReference type="OMA" id="MTPTMGW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005995">
        <property type="term" value="P:melibiose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14792">
        <property type="entry name" value="GH27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000202">
        <property type="entry name" value="Alpha-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000111">
        <property type="entry name" value="Glyco_hydro_27/36_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041233">
        <property type="entry name" value="Melibiase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452:SF75">
        <property type="entry name" value="ALPHA-GALACTOSIDASE MEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16499">
        <property type="entry name" value="Melibiase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17801">
        <property type="entry name" value="Melibiase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00512">
        <property type="entry name" value="ALPHA_GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035773845" description="alpha-galactosidase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha galactosidase C-terminal" evidence="9">
        <location>
            <begin position="302"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8072917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="382" mass="42472" checksum="69C95058896D79D8" modified="2022-10-12" version="1">MQKYFFICVILITLNALDNGLGRKPPMGWNPWNKYGCNINEEIVRQTADALVSTGLAAAGYIYLNLDDCWQSARDPVTKKIIADPVKFPSGIPSLVQYVHSKGLKFGLYSDAGMQTCEGKPGSLGYEEIDAETYAEWDIDYLKYDNCHTDGTSPKVRYPPMSAALMKQSKKIYFSMCEWGLEKPWLWAPPIANSWRTTGDISDHWYSFIAILEEQANLAQYAGPGQWNDPDMLEVGNGGMKTHEYQAHFALWAILKAPLLIGCDITNMSQDTKKILMNREVIAVNQDSLGKQATRVKKVLTSEVWAAEVADNGAGVVAVLFNQASIQESITIEFDKLGISGPQNVRDLINQVDLGQSTVSYTCQVPGRSAVMVKFRSVQSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M498</accession>
    <name>A0A8S1M498_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0550024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M498"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="82" mass="10087" checksum="A41B1413EABFA66D" modified="2022-10-12" version="1">MNSIKHSTNYRHPFTFNQWSSWNQQNKYNYGDYEYFYTKNELTQRGKQNKQQVVDIQIQLQMIWLENQQGILQSKSNKLKYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M9F6</accession>
    <name>A0A8S1M9F6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0520191</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M9F6"/>
    <dbReference type="OMA" id="IWCKAAS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="171"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8074285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="40413" checksum="816EE7DEDE778C3C" modified="2022-10-12" version="1">MQNNDIILKCSIPNHDALVQFVCFEPYCQDTRLFCNQCKTNEIHQNHTQRCVELPKLFDYVVKVGQECDDLLDILVKQWNSINHQFLKFIQGIKEKYLISHSQLILLNISQLNQTLEQLINFQSYKSLIKNSIQNNLFDFNEKIEKLEQDLRLHEKPISFHISEQDIKKSLELFQKGYELYYDNEKYDEAILILDQAININPNNQLAIWCKAASLRMLGQYKKAIIWADKSLLLDPKHSFSLYCKAACLRMLDKYDEAILWANKALAIDPKDCFALSCKGSSLRLMKRFNEAIKVINASLTINPNHFDSLKEKGYHKDHLYQQVHVYMMNININKQFYFMNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDF4</accession>
    <name>A0A8S1MDF4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aspartate transaminase</fullName>
            <ecNumber evidence="4">2.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Transaminase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0560043</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000057">
        <property type="protein sequence ID" value="CAD8076311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDF4"/>
    <dbReference type="OMA" id="MQAVSVK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000066">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="89"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8076311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="469" mass="53067" checksum="68A5B2DB686D7FD3" modified="2022-10-12" version="1">MIFHKIIRNIVIIYSKNIIIYNLMTKFGNNIIIIRKFVIINVDINLYQFMQQANQLNSSQQHIFSHLTQAPPDQIFGIMNAYKADTSDKKIDLGVGAYRTDEEKPYVFDVVKKIEQEIINDNTLNKEYLPIEGLPDFNKGCQMLLFGKDNPLIESGRIVTAQCLGGTGALRVGFDFVKRHLVGDVYVSNPTWSNHNQILDRTGLNQVNYPYYDPNTKGFNCSATLDCLSQAKQGSIVLLHVCAHNPTGVDPTETEWLQIADVCKARNLIPFFDCAYQGFASGCIEKDAFAVRKFAELGFQMIVAYSFSKNMGLYNERVGALHIVTPNKEISAIVLSNLKIVIRTLYSCPPAIGGRIASRILCNEKYFKEWIEELNTVTGRIIRMRTLLKNELDKLNVQGNWDHITKQTGFFTFTGLTPEQCDKLTQEHHIYLLRSGRMSMAGITSKNVSQLAEAIKIVVQNECDILLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEW7</accession>
    <name>A0A8S1MEW7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0510281</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEW7"/>
    <dbReference type="OMA" id="KQVIFIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035785442" evidence="2">
        <location>
            <begin position="20"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8073864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="354" mass="40806" checksum="635CE9F8308D8D7B" modified="2022-10-12" version="1">MKQVTFIFILITLTIQTQLQTKVDSVMAQMDKMALKNDFSKQLAGLIELKMLQSSYVEEVLKEIKVIRDQLIADQSVEDQEYAKKIGQLNVEIEILEIQTEKLAKELQRLNQQIGELNEDISKLIGTQQSQEKQLSTLNSKEEDIRNQYKSEIESLKQRTTNNIKSIDGLNEMIAKLQQAVFAEQNKTTILSQQHAKQYVDDLRNSLGPNHPLTALVAVTTKFDVPTVTRIIQLLENIRDQRIQENAGADEYEAKVTQAYQVTLKEVTEVRERLSADYSRTLVTLKRRNEENALSTKSRNQIQKDLPIAQDLLQQYRNEREIVQSNYNLRSAKRENEVKIITQAYTIVAQQVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPK9</accession>
    <name>A0A8S1MPK9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0650132</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="MEFLYFC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="187"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1029"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1230"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1355"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1609"/>
            <end position="1630"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1165"/>
            <end position="1192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8081262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1667" mass="197660" checksum="028C0E1542A85ECD" modified="2022-10-12" version="1">MNSIIIKKKLSTLYVTIRESKSQSYLNPFIIWILYVGTRLSLILRVDQFNGQVYQDKQLMLIKNIINWFHFLTPIKSIEVDSFESLIVILIAVVHWLSSLITLFNNNQVRFFNQFQNQYFTYLQMLYLYPSFIHIHQLQYINENQITVIIICIIPIILNYSIFTYIQRNYSLPSNNPFTKRYRPYNYLVNLTEIITLVLIPYFDEIFQSIFLLIQFLIQLCDSILNQPFPQQMNLNYCIGSSILFYCSIFKCLSIYWTEMEFLYFCLIYIPFLTVITKKLLKLIYEQNLEDKDLNKFLILQIIDEYHQKNNNFTHIKILYILRTRYQILSNKNMRSDIQFNDYIFIMFNRYIEQLPIQEKYKDEDLHTYKIQFLYYTYDKQNLAYIQIKQFQIYQEFQSLYFSILEGFCSEIIQKRIKKQIQQQQGRLELQQIRQSQMLQEKCQSIIVQILEQKIKYWDQLLNGYENIEQLSDVSVSLSLKVDNLKTIVFKELNIQIFTLQKELFRLNLIDLRLLSQIFATVLNDYHITLQIEQRIEELLNLERNISTRNIQNIGLLNDDVIIIPVSMIKNQGQILSKNKQKLFKFFKFLNDDSYQQISHINQILPLYLQTKHDKFLNKFLQTGESSLFIISQDIYPIYNSGFSFSATLQLIPSYDQMDDYILSAVLKRSTENYDFILFDETGKILGLTESIHQILLYNTKSQIIDGLDNNILKSYIYFWFRDLLLLINDQQDVFTGQQTQQSALNIQTTIIQPINNLQSLIKDHETFRKQHFLTQTHTMKTEQDVQTDQKKQVKITCFEQSYVNQTNEFINDQLLKMHQNSVYFQVTFMVHFQRMQDCHPIFLLSISEYLQKEQKYTGTQSAYSSLIKTRTQLGMKSEFQIQTEQDIDELHQEFLINQTIINKLLIRDGLPNSRELDQKEDDSNIQNIIDRDQKESNLISPRSNREILIQKGLIEEDSDYIRGNESNIIKSSSQKDIKENIQQAILQEQNIDFAHEKQSKSSATSDKTQNSIYNLMRKLQYTQQFQTSIINAIFLTNILTVFLIIIVSVELSIVRDHTNQLEYSIPLVRVPQRFNRLYCTFLTVGQLELQSKLLNQSYGEYYDYRIKNESASKRAEIQSLMTEILNEFSSMEHMQQLPLMEIRIINEYIYLQQNASMIEFDSLVNEYTDQINQYLQNIDENTENIKQVQILLQFLKGNLINQLDLTIAIVNQIEDNFYNLISINHMEELIFLIIFLLTIIAMLILQFRQWLQPYKYMQTILLLIGKISERDIELSGSRVYFLLEKLNRNNSTWKNINYFRDFFWQSKRSTSYKTLRISSSETTKNKNNVKNQQQRAKQTSRIQETSFSIKNIQIILLLLCLLLSSYAISAFVIMQTNMNNSQPELNIAMEFVKFKQDLDGVMIICQLLKVQQILIDETMASGIFKMNPELNTRDKYFQTLSTELLAEFSPLIIEMDKIYSTIYNNVIESKKISEENKQLLLNLYEKDLCEIIPTILPFCAYENDQFLYFPNFPAASSNLNNKQIYKYGINGIYQQIISIFNTHYSQEISGLKDTNLTNINQFLQSPEYVQTILPYFFDLSYAILQFYYTIIQAALDILENDYQMVLQFYIIAGVGCIIILYLVIMLRAITLQKRVRLIRQVLIVIPHESLQDQSILNSIRKIDRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MRK9</accession>
    <name>A0A8S1MRK9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0670001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000070">
        <property type="protein sequence ID" value="CAD8081982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MRK9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="14928" checksum="2A2CFA64A883D6D9" modified="2022-10-12" version="1">MMPLAKESKKNLKKQLHLGKSQNIFLMYFIYWFSLSFWCRISAYKEGFNNKNSLSRFGVMKAFKSGLGQLNITFDKLEDTRHTSFSLILQSVEPIFKANIILKNFDKNTTIFSKKGLIIKRYARGSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MTK9</accession>
    <name>A0A8S1MTK9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0690089</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cystatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MTK9"/>
    <dbReference type="OMA" id="EVDQSHH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001713">
        <property type="entry name" value="Prot_inh_stefin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414:SF21">
        <property type="entry name" value="CYSTATIN 14A, TANDEM DUPLICATE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414">
        <property type="entry name" value="CYSTATIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="domain" description="Cystatin" evidence="4">
        <location>
            <begin position="23"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8083059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="85" mass="9646" checksum="AC02B16C3352D899" modified="2022-10-12" version="1">MGGWAVVEVEERHHQVLAVVHHGINQHLGSNHQTFTIIEVRHQVVAGTNYQFIVQTEDHKKIQVKVFEPLPHTNQPAHVTAAVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MUA1</accession>
    <name>A0A8S1MUA1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0710067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MUA1"/>
    <dbReference type="OMA" id="NAIFFLQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="26445" checksum="A83E56E57A0E60EB" modified="2022-10-12" version="1">MDIYHLRLENYQPKQSFYVMNKILKLLIALQMLLVFFRIFVRNQEPFFLSDCLNACMMMLTYCQKNYCLALITMFYIFSLIYLNAIFFLQFVEYKLPFFIDYTKNFTLMLIFSTLLYIFESIMLYQVYKEFKGIIYDINQEYLENVQQDQQQHLEIEMLSQIQEQSVQQINSNQQQLAEQEIENQSIQEPNIAAESLQRRMNNQGSRTQQELQQQYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MV23</accession>
    <name>A0A8S1MV23_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0650089</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MV23"/>
    <dbReference type="OMA" id="SNLCTVW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="210"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="253"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8081176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="737" mass="86282" checksum="0A2D1523B6EAC20A" modified="2022-10-12" version="1">MNKAFQRKHSDNSILKGKTQHHTQQRSPLSTKENDTKFKYPEVLRRKESKPLQVQTNQSDMRSPSAQSTQSPINKSILITHRSQSMFQDAQYIDYIITNKEVNLFLKKHFLHEHMHTNSSRNTNTKLICDSWDKVPVTYFIKALFDEYPEIIEDHHLNEKLNIKLKTLIKDLISEDGQLLSLNKLQIHTRHLGLLGLLLTALRDIQDAPTVNHNTPHNQSISHEQDISNTQQQQSKFHKMASEITQSAENRINELVNLKLQQFTIKQKELEKQIQQLKQKATENFDLFQHEAKNKQVITNLTQQLQQKDQIIADLEQKLIKKWMNSSIEEETHTNKIAQPKASPIKIQSPSQSPFNDSNQYLQRMISNLCTVWDLGCVNEDYRFAQILTAQENNQMPYQHFQLIPIIKTIPGFNDFIFKSQNNNHIITNFIQNCMNNDNNIILDLYYEIITLLPNFKISDQDQYLINVMQLFLKLTVSILQIKNVFNINKLKEWGSIKRQLTEQQNQFVKNLINQIVQINQYSIKQIPQSKLWNQQQIYHDQYVSEYNNRLIENQVINNILKKQLNQIHNKQNTKLSLIIRIIQCFYYNDVEELIGLMQSIQGETDIDIYLSKLILDCDCINLILFPSLFNPKYVLKSITNLLQFKCPGQFYLLYTKQVCQIRNIDLLIELLTKMHNTMNSDVVIKLWDVLGAIAKSAALKECKEGLQFIIRLYSNSHQATILHNIQQVQKNLAIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWF5</accession>
    <name>A0A8S1MWF5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0720161</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWF5"/>
    <dbReference type="OMA" id="QIGHIEI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="471"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8084590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="761" mass="90493" checksum="4ACAC42F3D810D09" modified="2022-10-12" version="1">MWDSRQKNNQDLLIVNELSEYSSVSLKKTLLQGSQNQIEMMPLKPKPIQIVPLSEEYAYTMTQHIQLIGDLAAIDKAAVCTQNWKDTVRKQFNTQTITQFKTSYQINREIQLLTQLKRTYVNLMPIIDPQSSFLSYWKIPVLIITFIVFIEVPLIIFFGDDFYYTFHSATYIFFRSVVILIFMMDIFIEFHVAFYKYGALVKNRRRVALHYLMGSFTFDFVPLIEILITTFSYTMANVYTFHLLFLLKMYPVYEIDQRYQDKLQVYPKWKSIYMIVRMIIEIVYLTHFFGCIFFGTGMYMLNHYDNPDEGKYTNSWLTFSSGNFGTILYYDWTKRYILSTYWAFATLTTVAYGDITPMNEFEIIVAEVAMIAAVFLVAFNVNNIQQTFVDYFDEKRKYNKEIVAITKFMRSKNVSEKTEKEIRNFLEHYFKEKRNRDQYLEDQVFQKLAPQLKNRLMYEAYQPYLYQVPWITNNFSDDLMRELSQVMEEVDYSKKENIFLDGDANRDDNALYFVQIGHIEIFVNVPETEKQEVIMAHLKSGARFGDYAFVTGLQRKASARSKKYSQVVRLSRTDFMDKLKLFPLDHQRYCFLRDEIIFNNAYYELGIECFTCGSTDHMSIDCSLTHLSIGKEIIKSVRKSIIEKKLKKLTLNIQQNRNYQFQRNKKQKIRIIKTKQPEQAELDEYNSIINKKIQFEAFYSENQIIIESYTIDKIDNFVNYYPNFNIDFLISKNRKKEKLVFAREVRQRSVSQIIRKSEAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWI4</accession>
    <name>A0A8S1MWI4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoglycerate mutase</fullName>
            <ecNumber evidence="4">5.4.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0720245</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.11" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000075">
        <property type="protein sequence ID" value="CAD8084758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWI4"/>
    <dbReference type="OMA" id="GKTGDAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01039">
        <property type="entry name" value="PGAM_GpmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005952">
        <property type="entry name" value="Phosphogly_mut1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01258">
        <property type="entry name" value="pgm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11931">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="87"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="114"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="3">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8084758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="231" mass="26649" checksum="D3A5B1157D3142DD" modified="2022-10-12" version="1">MKKLVLIRHGESILNKTNSFGGWLDVDLSIKGIQEAQHAAILLQQNNHNFDVVHTSILKRSIKSANIMLETMNSLWVSQKSSWRLNERHYGILQGMNKKEASIKYGEEQIKQWRRSFSQKPPQSLDGNSESLEDVTIRVRPYWEDCIAKDINQNKQVLVVGHSNSLRALLCIIKKLSEQQLLELNIPTATPLVIQFNDRLQYQDEFYLGNQEQIKQKIKQVANQGSLKQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MX18</accession>
    <name>A0A8S1MX18_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HEKINFY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1025"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1226"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1332"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1543"/>
            <end position="1563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1605" mass="190748" checksum="DFCDD9A16E5123B2" modified="2022-10-12" version="1">MMIILKHYTIIPKRLSLFLQVVLLIQPIFMIIETGLQMHHTDNSLPHLIILLLFRQDLLLLDQNFNQIQLYLIVLAILLFQKALQIPITFYLYHIKDRALSFQKIDESNLIQKALRILATIMQLQLTALHLLSSTFCMMSLTYAIRNGDQYIHLFLSTFTYILWQVEYIFTFSLCSTPLTYKYHYLDITKVTILHYIIYSVQQLQIIIFCVLQDNQEIRIVNIILLLIEIISQLTNQFINHIYIYKESRQLLYFINAFKFTFSCYYFQPVVNSQYLIIPMLIYPIILYSFITLDSEINNSLFANIFKDSSSITLLIHQLKMILNQCDINIKMNPLKSSLINNFHRKNCDKSECICSNKLYILEISQANAITQAILKQFLKSRINYIIDTKNLQKNAQINELLFVQTLFYFDFGWLTDCIKNLTQFLNCFENNQNIMIKNTVQQTKNLDDPQNQSFTKKDRSNQQFPQFIYVKIQLDMTQYCRLNYILHLAKFKLKSFLGTSMYAFQQLMVSDFIISFLNYDKCLREIQTQTLNMIHEKINFYHSIMKDEKRNLNHLAVQTTKICINLSKMKQRLKQIYDQYPCKSMQRCLCFFQSELLNNYYEANKTSSITSMTDDKIFRNKKIRNYEIQVEQTAFIILSIKGDLDDFNIELGSSYLLQLIGYDTYKDLQFYQLLPTFMRHFHPIIINNFFINGQSRFYKSFNQSFIQTKTGLLKTLPDQKCFMLSYGSKRKLIFSENFFQKIGFDQKIILNLPSIVINSLNLQYLIPNFPIDHSETETSQINTEMRFLIDKRLKELQIKGVDLSQYILDLQQWENEDQVCLYNISVMITKRFIEQQSYLLIEFSSITRMTKFSQDVSKFQTIQNIDMNSPFRSPIIQQRKNSVSIASLMNLSIEAQAKKVKVMDIRKKDIQMNDLSEDEVACNIASFRKDELQNHAPYQSQRQLLSERKNDTQQEFFNIDLLKMDSNIKSSLEIQKSQISKKKIDQNDDIQSQQSSIGGVRESQLFKKFEMIEKIIKNKKFNTMIIYIVLLILLTMFWISYTIVVVTAMSSQLLQFIQEIDMISLHASIMGPHDMYLSIKVTVSAYQQQYREKFIDQTTLLKLIDPLFQFNAPYYFDLQNSFFNILKNDHLTEFFIDKYETVYFMGNNDTVIYSRNLSFREELLTIINAQYQFKRIFDERKNAAGQPCQVFQFANYLNLQDQLEQLTNEILVYSKTRSINSNDQWLILWIIYQITSFLLIIALVIVRKRMLKTYEQLLSLFYYSDRNSLEQEILKLKHLHQLITTQNQEIIQKYEFDFKQREDLIKQKKNKVHQQKIEKHNLRKNNQLPQFLTFLRIFIMYAYFLIYSVVIYDQTKNYLVKYRQTTDFYRLVQDMRFRSGTIYVYREIFFRWSNFSYLTTNDLDRLYQLVEKSQNIIQQYLNDQSDLQNDNYLLSDNYINFITQVENSNLCNFIDPQYINLTIYCSISLDGVLLKGMIPSLNYISTAIRSQQAINNFTKRIEVHFYELEGAQIICQVFSNVSQKLQQGMIDLTHKFNYTNEILSYIFLVTQLSISIVILTCFKNSLINEFTMYKKSLYLIPIQVLLGDNSLERALRQYEFTEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N295</accession>
    <name>A0A8S1N295_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N295"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="18157" checksum="DD4A3D2CA0ED2B70" modified="2022-10-12" version="1">MSEEEQVDGKFDYLKAKYVFRFPFTVTALKWGFGLGTFFGIHQYIKSRDGMASMRWFIWGNVLTTLPIWGFFMMKYSFYSSALRKFESEQSRQYEIQGLTRVYLANKMGIDPECSDDDLLKEYQKKSEKIKEKYNLLDELLVGVDFSQFEFPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7U7</accession>
    <name>A0A8S1N7U7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0820026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7U7"/>
    <dbReference type="OMA" id="YENSKNE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="365"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="507" mass="60830" checksum="FA8DAC0CE6F4590C" modified="2022-10-12" version="1">MKTYIFRLKVDNAQDLHHSKLLWQNLDSNTINIAKLQSVKDQVVFFQIETKPNIIKYWYKNSQQEEEQQRRKINLKYGDRIEIDKFNCYNVVLKSEIKSQDHLQYTSMYEQNEQRRKILSFNKKNVIHVPYQNKNLKFLFEHQKQDQNYIEVLFEVLLSNIPQFGNSIQLQIKENDLEYCLFCDFRLYKSEQNIQLKILDRKLIGSDDIIFYKYQTFALEVEKQSTRLDDLIQKSQILDQNLKSVNELNSQFTQIGNFFSVIYQSTKQTIESQNIFSTQIIQNQSKMQEQQLSRTQQINKYDNEKKQNLQQQQNNQIKKDIELNKIKENQKTQQQDFHQSDDEQLESTAPLQPMKIDSNMDQQDYQNLIQKIQEQDQMIKSLKQDYNQQISKLKLSITDLNLQLTKEKEEIEQLKQNNSLKFIQNYRNRRLNTSVSQSKLQSKSTIQFMNLNPIIQQAKIENFKDCQSFRQTNNCMSQIDQNQNNQKKQKIIIKEVNNHNQCFKIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEC7</accession>
    <name>A0A8S1NEC7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0870060</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8090960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEC7"/>
    <dbReference type="OMA" id="ATDWIAL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05580">
        <property type="entry name" value="STKc_PKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="16"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="271"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="296"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8090960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="323" mass="36636" checksum="E88D0D47052779C0" modified="2022-10-12" version="1">MSTGLAKPKKLKLTDYEVFQTLGTGSFGRVKLARNKQTNKYVALKSLKKAEIIRLKQVDHVINENTILGNLQHPFIVTFEGFCQDPRYIYLVLEFVSGGELFTYLRSIGRLDTQHAAFYGAQVASMFEYLHTKNIIYRDLKPENLLIADDGYLKLTDFGFAKVVEGRTYTLCGTPEYLAPEILLNKGHGKAVDWWTLGILIYEMNAGIDPFSDEDPMAIYQKILKGKVKFPKSFDKNAKSLVKHLLVADLSKRYGNLKNGAADVKNHRWFGNLDWNLLTQKKLPVPYKPVVKAPNDTSNFSSYPESDTQSPALKPADDPFLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHP6</accession>
    <name>A0A8S1NHP6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0890162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NHP6"/>
    <dbReference type="OMA" id="SIAEMVY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000051">
        <property type="entry name" value="Protein phosphatase 2C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="149"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8091990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="426" mass="48457" checksum="352010E263406763" modified="2022-10-12" version="1">MFQKFGQQLKPIINRSSSENRKEQSVTKPNYHFTQKRERSESRVPQSTVEDQNQNNLKQQSNFNQNAPAQRLTVKLPDLKGYQKQMEKSTSGADLTKKPPLLKNRLILDSKTKQQPQGQQTSLTQTLPIKSQREDIQTSQQQGKNKLKISHYSQSRAGFDGIQEKTNQDRELCLQLDDYNYAFAVMDGHGMDGEYVSSFIKELLKYNLTKFYKVFDFQKVFFEMHQKMKFQTEFGCQFSGSTLTVLLIRENTISCGWVGDSRAILVKKNQNNLNVIELSMDHKPHLENERQRIEQNGGVVDTYHLPNGAPIGPSRVWAKGAQFPGLAMSRSLGDTVAAAIGVSQTPDIKSLEIDNKEDIFIVLGSDGIWEFLDNQSIAEMVYPFYQKNDVQGACQKIIQEAVAGWKAHSEGIDDITAIIIFFQNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NIB0</accession>
    <name>A0A8S1NIB0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0890093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8091852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NIB0"/>
    <dbReference type="OMA" id="GIQHKTD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="207"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="104"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8091852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="242" mass="28224" checksum="BD7601F9F34B4B36" modified="2022-10-12" version="1">MKVEVRPSKQGGLYSLNLGQNNGPYLPKDIMLTNQKSLKLLQGDEVSKSVSQVLTRAGMGQLYEPPDIYDEKQKQFVNLDEKYKTRMSASDNVAAMSTLYTTSIKQLQESLLEYKELIKKTEEELKKMNEKINNDNITNIRELQPMLKSFHSKLKERLHDEKNENYKLMREIEGLNRDKLQIQQSILFSHKRIMELEKLVGIQHKTDSLYSQKIEEEDGNENEEEIDNSQKDQESQLSESYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NKN6</accession>
    <name>A0A8S1NKN6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0840014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000087">
        <property type="protein sequence ID" value="CAD8089585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NKN6"/>
    <dbReference type="OMA" id="HEVNSKK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="44773" checksum="FA7D3E30D1F42E32" modified="2022-10-12" version="1">MFIPYKKNKGYKIPVIFEFGQQNTRVGYAGTDGPEVVLPSDLLMDSEGNMKFIEQNSTYQPIGFDVKNILQGGQIVDFDIWKEYVEHILNNVLIQERHFPFFFVENGNVKKETRNKIIEMLFEEIDVSSVFFHRNTMLAQYIVGKDNVLVVDCGASHTTITPILDGNVIEKGIIRGEIGGEYITNKLYDIWKQQGLPFSRFKNYSQLSPSLQMYGDLEIVREIKHQCVKLYDQPIENLKFNPTVDNQAYELPDQTKLLISEEMYKYPEFLYEPTENFEGLDKALETSLEKIDTDYRKELVQNVILIGGASCTVNMVERFQRTINNLKIFGLSSRSKVQTLPKFFDRQHASWLGASIVASSQIYDQWTLNKSDYDEHGSSLIEKKMFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NNM1</accession>
    <name>A0A8S1NNM1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0970082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NNM1"/>
    <dbReference type="OMA" id="CHEGMYR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="43265" checksum="FD7C833D5CC02F52" modified="2022-10-12" version="1">MQLNNQMDAIFQQEKEQILKVFESKLHQLQLQVQNKQYQDGSEYFGELINDLRQGQGVYYYFTGDVYAGQWVNDLFDGDGTYIFASGERYQGQLKEGKKHGSGTYYYLNGNSYTGNWANDKKQGRGIYVYAQTNEKYDGEWNNGLRQGYGTFTYQCGVYDGEFNNNVKEGKGVLKYKSGAILDGEWKKDKVEGEAKLTLPNGDYYKGTWIGSHMNGYGRYQYQSGGYYEGWWKQDLFHGEGMLTYENGDIYRGEFQNGLKHGKGIYLHQNGDQYEGEFKNDMREGTGVLRMKNGDIYEGEWSNSLKEGKGIYYYSNGDRFEGNFLSGKRSGHGILYWSEKSSVNGFWVDDIINGQGVLMKENGDKLQALFKNNKLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPX1</accession>
    <name>A0A8S1NPX1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0950091</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KPVQCIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052798">
        <property type="entry name" value="Giardia_VSA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23275">
        <property type="entry name" value="CABRIOLET.-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23275:SF100">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6556"/>
            <end position="6579"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="369"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="402"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="783"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1176"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1211"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1619"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="2064"/>
            <end position="2100"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="2536"/>
            <end position="2590"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="2941"/>
            <end position="2972"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="3758"/>
            <end position="3792"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="4213"/>
            <end position="4243"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="4245"/>
            <end position="4275"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="5018"/>
            <end position="5053"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="5505"/>
            <end position="5535"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="5911"/>
            <end position="5941"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="5943"/>
            <end position="5972"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="5975"/>
            <end position="6007"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="6009"/>
            <end position="6039"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="6463"/>
            <end position="6493"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="6509"/>
            <end position="6541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3014"/>
            <end position="3398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="3033"/>
            <end position="3047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3050"/>
            <end position="3089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3095"/>
            <end position="3134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3158"/>
            <end position="3167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3209"/>
            <end position="3218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3227"/>
            <end position="3236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3248"/>
            <end position="3257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8094282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="6687" mass="774227" checksum="C4EB2D1029AABB22" modified="2022-10-12" version="1">[encoded data, 6687 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NSX8</accession>
    <name>A0A8S1NSX8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0930017</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000096">
        <property type="protein sequence ID" value="CAD8093331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NSX8"/>
    <dbReference type="OMA" id="YEKFVCS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="95"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8093331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="417" mass="47851" checksum="435EF8DD676A1CC8" modified="2022-10-12" version="1">MNTTKFTLENYVRSCILKSTNQNPIRLMLYPNTKFIDIIKLIQIVWRQNVQQLRLFLIDGTQIQEQDLINIKQGTIVYTQLKNYENELIHLKLDYDIIKRVGEGGQGVVFLGKHKGTKQLVAIKKINASTQQDQVLETLEQESLILKQLDHQNIVKLYEKFVCSKQKEVIMILEYLYGGSLLTQANSKLTEIEAKNFSHQLVDAISYCHQKNIIHCDLKLENIMLSSLSNKKIKIIDFGVSNYAGQPLVKENVLGTLSYLAPEVLSNSIKYIQPGQDVWAIGCIIYGLVFGKLPFDGNSTSETYRNILQCNYQIPKKCVSKDIIHLLSLIFVFNPKDRANIYDIQSHNWFKEQSKIFKLNINGVNLRIGRSSSVVKMQSLRKSNDEDQGQVMSQRKIGCSRRSVTRLDKVSFLVKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NTN3</accession>
    <name>A0A8S1NTN3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0970028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NTN3"/>
    <dbReference type="OMA" id="MGNGTCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="180" mass="20369" checksum="B5FCD5EBEEA6F7D0" modified="2022-10-12" version="1">MGNGTCKQQPPSLATIDLIDQKQHLPNSQQQSPTKPNIDSINPTIAKSEDEGPNISIEVEEEGCSICKVSQADSPTKERSKKRQSYEDQFFLDLLHQDKTLFRRSSQGQSISPVRIIATQHKKNKTSFIIEHTELIKLRQEEVDEISKPKSILKSSNLSEKSFSSQSQKKVKFGLIYKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYD9</accession>
    <name>A0A8S1NYD9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0960175</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="WNKGQCL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="32"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="66"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="168"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="202"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="236"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8094705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="32972" checksum="9A434D7FB291DC94" modified="2022-10-12" version="1" fragment="single">MYNQGINLAKELKYDESIQLLDKSIQLDPNNYEAKKRKGQCLRILERYEDAITWLDKALLISPKDVNSLWDKGYCLTMLERYEDAIAWLDKALLVNPKHVLSLWNKGQCLRLLERYEDAIIWLDKALLVDPKHVLSLQNKGYCLRLLKRYEVAITWLDKALLIDPKHVGSLWDKGQCLTMLERYEDAITWLDQALLVDPKHVLSLWNKGQCLRMLEQYEDAITWLDKALLIDPKDVDSLWNKGLCLRLLERYEDAITWLDKALLVDPKHVLSLQNKGRFXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZ74</accession>
    <name>A0A8S1NZ74_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0980141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZ74"/>
    <dbReference type="OMA" id="QDEWQVI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8095376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="353" mass="40214" checksum="D5C0A07E90A4528D" modified="2022-10-12" version="1">MQSQFSSLYPQFNDLEEVRQFITSKINTVDLTLQSPPYLSSFFQPLRKQAFQNYIAAKVSDQNQRKMAIYKFIQLVDIEILSLFSEQIIKPYQNLILVRHYGIPQNLDVEKLKLDNQTLGALNDLANSIAQWNLQIEIAVDNITRVQLYDLWIPSEKIKEAQLTAEIKGILSPSDDFDFKTYSKHILIRILAQGAEKFGKYLYSNGLSESNLSYGAALMIYHALVGKSALPWNVIMPNVSLKWLFGGVIGSLALGKVSTYLEKCDQVQQLKGCAEVFQDITNFLINFNETLGAAISDVLLTVNEEQEKEKKQNLTTLVDSFLQSPESIKDSQTEIITCEDIIKHEQDEWQLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZW6</accession>
    <name>A0A8S1NZW6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1010099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZW6"/>
    <dbReference type="OMA" id="PICPLIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="150" mass="17715" checksum="88D14058BEE44859" modified="2022-10-12" version="1">MLLNKGLYKHQYCLSLHSNPDPIQITFNFEESQPICPLIKMPSQKIEWLQTKLPSIKTRTQKPEVQTERPKQQKRKCHTRIASQVKSIPTSPFTIIMRTNNQSPIEYDNNTKTVRYEQFQQQQYLVNKYLAPRKLGLHRGINLFNFGLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P134</accession>
    <name>A0A8S1P134_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1000165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8096225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P134"/>
    <dbReference type="OMA" id="ITMIEKD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="146"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="262" mass="30722" checksum="852637B4206006C1" modified="2022-10-12" version="1">MSSNIYLTQTQIHDLFKPCETKKIELKTLEIPSFGDDNSNQLSPEFQQEAYCFLSSCKKDENYTTNPTNNTNRHLSQDSIIELKDDTVRISKTEYESLKRDAQHYKALMKLNLKLKANKQVKNQTNTYQSQIYVVQECNKKLQGHIYELLQTIKILEAENQQYSEDLLILRLQIQDQLKIIQQGKIDIERLKITMIEKDESIIELQENYNKLFKETERTKNSKSAQRSTYVSTHTSSPLFKGLSRICLNNYCNTSFVIEQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P2G5</accession>
    <name>A0A8S1P2G5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1000030</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000103">
        <property type="protein sequence ID" value="CAD8095974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P2G5"/>
    <dbReference type="OMA" id="THSTMPD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8095974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="354" mass="40832" checksum="21BF45C05C6D57B1" modified="2022-10-12" version="1">MNLKNSEYKMISNLGSSSDHIVFKAQHNLTQDVCAIKIEKKPGIGQIENEILILQQLFGIEGIPKLIHNGKTKDLKNYLIIPLLKCSLQDLAKSNLLTLNSILAIGLSLIRTIKQVHNRGILHLDIKPENIMISSCQIIDSVEQILTPGNVQLIDFGLSQNLGNPKFLKETFIGSLRFASRQSHNKQQLGYKDDIESLLYVLAYLRNKKLPWQQTQQLAFQLMDIKKIGMIKDHVYETALLSQQFPPQFYQFQLYIDTLTHSTMPDYNYIEELFKQMLQINQIPISPRFPEPTAYFPKYNDLEDQEYIKMNESISIRDSETRAIIVSDLIKAYTTIKIKSIKDIPFRITIKQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5S5</accession>
    <name>A0A8S1P5S5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1070017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5S5"/>
    <dbReference type="OMA" id="TMNETYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8098390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="305" mass="36523" checksum="B12009918516457A" modified="2022-10-12" version="1">MLPLMCATPYVNYPQLNLQQFYMLPTSTYAMPIPMKETCDQACQFPEPIIEQDAVTNNMEIIAEQELTSLLRFLSKNISLLQDSVFEEFVQENLKTLSSFSKDIPNMIKKRYVQVNKTKEEMTKFIIRRCFLFIKSQIEYEEKEGMSAEERDRLFYHSFFSDDKEFMKNLNQESIDDMIPFRKDSKMKTMNETYLRKLFASERFSNFYKKFLQSFTEICNHENDEKIDNMTKQIIKIIITRDFAKIKSYRRFPWKDHEIIKCEQRAKEIYSKYSNYSKANTKNKFLSKLDSHTLDILEKFSESSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7S7</accession>
    <name>A0A8S1P7S7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1080171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000111">
        <property type="protein sequence ID" value="CAD8099106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7S7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8099106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="89" mass="10803" checksum="C3A8A418E3C038B5" modified="2022-10-12" version="1">MVPTQYSQYCCQKQIHFFKRIRTISRCCTNDLQKVSLILFHHWRRWLKCIEVIWWAESVNNSILSSPYPSLLARVKLIEVMDSYLDFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P8X5</accession>
    <name>A0A8S1P8X5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1090024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P8X5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094:SF25">
        <property type="entry name" value="SCP2 STEROL-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094">
        <property type="entry name" value="STEROL CARRIER PROTEIN 2 SCP-2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="19"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="111" mass="12548" checksum="84EB48984C49047C" modified="2022-10-12" version="1">MALKSAVLYEKMDPFIKSQRTELVKKINAVYYFEVSKAKGEPPEVQSQKWNRRKVGTADATFTMIDDDKIVMAQGKLNPQQAFMQGKMNIKGNMAAATKFTPDLLPKDAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCI4</accession>
    <name>A0A8S1PCI4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1120118</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCI4"/>
    <dbReference type="OMA" id="DDHEFIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641:SF19">
        <property type="entry name" value="NEPHROCYSTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_6G03870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="316"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8100443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="52579" checksum="D310CCBE4010F7C0" modified="2022-10-12" version="1">MSTGYERIDQETLRRDDQLNGLLQRIREDVMKGYIVEVKTRANTYNQLDNNSKQSSRLSSMSNSIVKQPMIQQNNQVKDLNEIAVVSQRDQQQMSNLSKTFIASPPKVRSEIVKPVVLNYEFSPDQTQNSIPQKEELQTPKTISQQLDDIEMNADKGDFQNYIKILKSITTQLQKSMIENDHDCNHMLNQAIQTVRTMCLLTRFYKQVGDFKNAIKQLKTIQKQFKILEPNLEGKILIELGKLHFLNQAYQNAQSSFYEALQYYEKLQWKSEIAYILLLMAKLHSWTKNFDLSKKLIYGAIAILKEFVPDDHEFIAEAYVSLGECNYIQKNTDEAIEFLMKAVNIKYKIYKDYKHLKFVEVFNLLGLTYGLVPDIQQSLNYFIQALQCFQQNCIQRAQILNNIAVIYQSQGDVDKASKCHFRAKEIYSTFLPSQHNQMQRLILNQTCISPPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PH77</accession>
    <name>A0A8S1PH77_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1180124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PH77"/>
    <dbReference type="OMA" id="QVWIQAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035931643" description="Cystatin domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="14299" checksum="ACD1CB26B6403166" modified="2022-10-12" version="1">MKILFVLLLLIIIKSEMLLVSQDNEFIDDDNDFVGGVFPTTDSNSDTYNKALQYAQEHYAESCKLSDLQWESLDGSKNQMVQGMLYYFNVTLKGKERQEKYEIQVWIQANKEQTAEITQCKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKE0</accession>
    <name>A0A8S1PKE0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1210052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKE0"/>
    <dbReference type="OMA" id="PAECNDI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="176" mass="20612" checksum="546EE631F8206FB0" modified="2022-10-12" version="1">MRQQKDGLFFQCQLYVQELCEPLNILELLDDQANQSISHSTKPENLEISNKTNKNDSSTKINREAKQNPYGSEWANKVLEKQFGVHLSQITQKKRPKWIIKKLKPAECNDIKEKSHSRMIYGIKTQEKLSRQIASKSNSRGRDIIVKNYGQSFSRRRQPLPTFDINFLYKLDQQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLV3</accession>
    <name>A0A8S1PLV3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Wntless-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1220091</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8103901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLV3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015643">
        <property type="term" value="F:toxic substance binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040416">
        <property type="entry name" value="TMEM181"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047843">
        <property type="entry name" value="WLS-like_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31918">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31918:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06664">
        <property type="entry name" value="WLS-like_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="414"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Wntless-like transmembrane" evidence="6">
        <location>
            <begin position="155"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8103901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="486" mass="58187" checksum="5A85E90B8EF9CDE7" modified="2022-10-12" version="1">MMINNRKKYLSYDIRLTKFDVDTNQQEYQNLNYTNSTGKYTFPIQNISEVELNLNVTINKNDYYDLEHVMVKEDEILNDMKYQLIVHSENQTLVLNETHSTSLKCRFKSQQIQCNGYLESIINNGYKELQFQLLIFHHNALAIHSQTLTLLYENNQYYTMILIFQVLFLFLIIYGIYYFFSNLSDYPKQIWPKIHQWLLILLIGMVLYNFPILLIRNHFGQFVHYYQNLVELFIQSGLFYFWLQIFELEQTQSSDDDGQINQKQFSIWNVLKLLLVILYTVPQALMDTFIFIMQEELMQYDPRVEIPFYNTYLKFVILSLIGYSVCYVFLLFKSFNRKEQAILITEPRINENEKQHDPFYEGQQIKESEGQGEIRNYKLLHVLSTICFSALVYYQLVTILEPIVLTSSSTINEWALINIYLCMIVQLYMPYNQPFEDEQQQQMYGDVEMDDYNIKSTRVKHKNLANKKFYTKQKDEQISQEDNILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMN5</accession>
    <name>A0A8S1PMN5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCR4-NOT transcription complex subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1230036</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNOT11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMN5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019312">
        <property type="entry name" value="CNOT11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15975">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15975:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10155">
        <property type="entry name" value="CNOT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8104169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="154" mass="18600" checksum="383C4543E423055C" modified="2022-10-12" version="1">MILNLFKPEYKIDWNQFKFLVEQNPQLVSDLLKKIIFNRCQCQRIFRIFNLDKDQYSDIRISELIIEEHKSPRLISQYVYNKMHRKLGRIQDQLSIKLTRQVRLVSVFIRTLIKQKIFDPEKIYVELQGFCLEFSSILEATQLFKTIKNAVQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQI0</accession>
    <name>A0A8S1PQI0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1270050</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQI0"/>
    <dbReference type="OMA" id="CIEGWFT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001467">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF3">
        <property type="entry name" value="RING FINGER PROTEIN 141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="106"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8105395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="182" mass="21130" checksum="A017FEFEA9BC6773" modified="2022-10-12" version="1">MGQQQTQEQRPLNDLIKDVATFNLLAKSPTIKQSLGKQLIAEVTKRKQDLFDSLPFVKKQDDLCIGIFVDKEYLQITQQKFYYCKQLLEDFSSILMVDPQKEDGTCQICFEKKIDKLLPCGHSYCQDCIEGWFTVKEQDSCPMCRAQLTKQKMKSESYVVPNEAELIDSFKEQLFLQFSSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PR80</accession>
    <name>A0A8S1PR80_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RyR/IP3R Homology associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1270109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="RRIFLNC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13715:SF99">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13715">
        <property type="entry name" value="RYANODINE RECEPTOR AND IP3 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2129"/>
            <end position="2150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2183"/>
            <end position="2204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2224"/>
            <end position="2244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2250"/>
            <end position="2278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2299"/>
            <end position="2318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2384"/>
            <end position="2405"/>
        </location>
    </feature>
    <feature type="domain" description="RyR/IP3R Homology associated" evidence="2">
        <location>
            <begin position="1765"/>
            <end position="1857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8105511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2535" mass="300164" checksum="1ABD77F0362C13E2" modified="2022-10-12" version="1">MTLTKKSIKRVVQLSMIESTIQSRQQTIKSYENNLDEEMMANIQCFQKLQDTSLLFGTSTFHLVHKDSLRFVMIDQESVQLVEFPTDYTVLHFQPVYQYQKQISCILDQETVTLCAAKSIQNRKCYLRLISKDQADQVSSTQINPTYLKMNLYSSLQENEDLLYAGDLIAINLSETNLFLNAKQPYDTFHEIAEYQIQRLQNQLTSLTLTKIIYEDLKVTFDDVIERIGMKRIQKLQSSSYWKIEKTNGQGGQIQFYQLVRFKHNQSSRYLELLNGDPILTNQLSKLSLFMLIPIDEGQKVLKKDSYFKIQHYQSGIYLQDHNNKQENRNINILRLRKIGDYEDTETKLLQYSVKIFIEAIELLQEINDQEIKCSAYDIKNKEGEIRLKIKFYFIYQKVKQILTYVSDFLMNKLIFNLSPNQKYNQISFIRQDSFRQEPLFPLICLLFQMLKPELVEQIASNDKDFFEKEEKFYQSTKTINRADRDEGRIIYYYSLQIKILQVQLIETINIICKDNIENQNCFFQFLPILHKHIILYPEFIAILNSLVCKNRFFLEDLSKIRKISSKKQNQNTFILEWLTEKVSLNSTAKTEIPFSQKTEILHFLAACCSFGVEAIFANQEMVFKTLINKKKNITLMKCVVDDEKNIMVTCNSKIGQVSIKFESYFEHAHKNSNPHFQEELNFLKAQFFLYSQLCLQRNYDSIKYFGEQFSDNSLVPLLNNNLIDPELRAHLIALCKNIYLDRDPLISQVKPTLIRVNISNSQTFFDNMFYEDEDGIFDKVELETKSLYYVILQFKEILSSYLRQMVKNLHEQQPQKVYNLLTLQIMRILFMLLQFGWFSKQSDEFNNNRMDDSKEIEFALEKLIQQLSLLLEYDFDYQKAHSKLKSKNVNEKLAKIIKIQSLKFFRKTEQKKINQSSIETIRDPLLRKLQNVKRVLNRFYNKTFSAQTNTSDYEIEIKIEICKIFQYLLDMRLDFMIDNAIAFFTNTFLPQILKKFTHSEKQQLRDSSENKSNLQHHIVYYRQKYLTSLQGLLREDLLKPGQKKFQQRGIKDHVQINRIRHFDRIIDRPFVEVLLKGFYFANDNVLQNHIVELIERYQNQRSEFCNYLKQIQMIMTNNLQIIYRTMYILVQKLKNNVQNSQIWLRLDIPIALNEKNEETIEKVLEKLVEIYTIISKNESNEKSVRRIFLNCGGYQVVYQLIVKCLSVIDVNMNIFECSFELTDYDEQKQYQQELVEKILSIFQACLKLIGLSVRNDQQNQEYVLIKLARPLLRYNMVNLGQIELINELFSNNHNLLPLVNSNDLYQLLEYVKLYGKYQSFIELFTSIVRNKQCQSLYKDIYQALVQCESKDINPFQDNYNFQKFYPNQPNIEEFRQSFLKIVDVVLINDDRAALSVKMLEVLDFTDLTSYFLEKTQEMRNTNLDLELQDSITKIKFVICRIIITLSKKKKKLLLIHENYFLQIISIEAQWLEQQEFLQISAKTYIVEGLLPLINTYNEYANKKNSFKTDIAQIRSYALSVLKQYSQLTKNSDTSTKYQVHRLVKAFDLKLPDERSESDSDQEGLMSDINGETFKTEKFEEYQITDGSEGSDQGCNDLELWEFFKKLFDNNPKIKELTETDQLAQSIWHVSDMFTSDFWGDKEKRQLILTNNDMLQKILAFLQFWKSNKASKQSAIFALKLLIQIIKIDQNRIKILDQLGGTKIILTLIWEENESENDYIIQLLILIKELMSCKQVQKTTLTFLSLAISSDKFFQKMEMLLTIEQDLKICTLVLQIMELMCNDNNKELQNLIRYQKNNKKSYDLVQQTINYLCSLDVKNNYDQLIQCLKTLKSQAYACRPNQTLVANSKFVEFSIKVLNDSSLKLQLDDVQIIQLKHQCLIILLVVAEINTKDDYIVQKLKKIVPKTVLIQHFVNVYSQMVNLFGEDNYSKLIFVNQPNFIMESGFNAYILYVILSELNYVVEDLEEFKKNNDEGQLVQDFLKDNILNELSRLGQSLMKHGLEQLNQIKQQFSGQVEEKMNIIKKHEMMHKAFQFYKINTLQVEFVKDDVLYLTYFPKQPCFYLLRQEIKKKFLDQADRTSTKTKIISLVNFVDSAYKFMLHQEALRDYFKKHKFLEILARRGKLWMQLAFYNGFLINLIIILSYTQLAFPPGYKPTDSDVIQYYRLNEPRFLEDYNNTNTKYYLMILAYLNLAINFMVLFFYLIDKAPVKLQFVWNAWHASSLIGHMISGLIKVIISIIVLVIDFDIIYYILQIAFNIIGITIHPFFLAFHLIHILYLEPLIPILKAVWLAKFKFIGLWFTIVLFEYWVALISYVYFFEFFITDKNQNLCNHLWQCVYVTFDWTFKTDGSVGGRFMENIGEQAEEMYLSNLNNYYGRFFYDNIVNIIIKLCIINVLLAVIIISYSELRSVQKQKEKDQNHKCFICGIDRLVFDRTSQNTGFWHHIKVEHNIWNYLFYMCYLRMKQSDFNNGVDNYIRAKQQACDYSWFPIRRAKALSLKLQLLESVNQKNATSKSKLIGLYHSLRRLVWIHKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRN6</accession>
    <name>A0A8S1PRN6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1290101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRN6"/>
    <dbReference type="OMA" id="EYYFARQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="139" mass="16953" checksum="6CBE8A6930E235A4" modified="2022-10-12" version="1">MAKEVNREEYARQKHYKDKKKQFAIDKLKTEFPQSLDQYGQAFQQKLKDCKCEKDPDPKKGIIHKEDCSHDDFTIHQKNQKKLQKNYVYYKTEYNYCRGYSPEYYFARQIYKEKQADLLKIQIKQKILKGKDFNDDNIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSS2</accession>
    <name>A0A8S1PSS2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PPRIM_AZ9-3.1.T1290071</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PSS2"/>
    <dbReference type="OMA" id="WRTRPPE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000299">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000383">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000828">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 27" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="26"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="329"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="366"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="400"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="436"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAD8106044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="470" mass="53580" checksum="A92164BFD4C209C0" modified="2022-10-12" version="1">MDNNIVIKKQWFIKSSATKIEDAYEFDHKKLLGQGTYGQVVKAKLKGSKYYRAIKIIPKSKVRNPDRFRREIEIMRNLDHPNIIKLFETFEDVRNVYLVMELCEGGELFDRIIDKGHFSENEARITILQIMQAVNYCHQNGICHRDLKPENFLLLTKADDSPLKVIDFGLSVIFHDNHVEKLTQGKVSMTTRAGTPYYISPEILDGKYDESCDIWSAGVILYILLSGVPPFYGNTDPEILDAVKKGIFTFNIPEFKKVSEGAKDLISKMICKPEKRLKSHEVLQHPWMKQQQPGGSFLSVNYQSLKNFTNFNKLKKVTLTYIASQLSEQEISELGKLFKQLDKNGDGVLTMDELTHGLTGLKKESQNEIMSVIKSIDTDGSGTINYTEFLAATIEKSVYMKQERLFQAFKMFDLDGSGKISRDELKQVLGKNGSGFDDNTFKALIADADKDGDGEIDYNEFIEMMDKMKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PUM0</accession>
    <name>A0A8S1PUM0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">guanylate cyclase</fullName>
            <ecNumber evidence="4">4.6.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Guanylyl cyclase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T1310146</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">In the C-terminal section; belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">In the N-terminal section; belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDM010000134">
        <property type="protein sequence ID" value="CAD8106752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="SVTFFEG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000026">
        <property type="entry name" value="Guanylyl cyclase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000045">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="308"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1033"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1065"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1115"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1154"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1179"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1221"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1350"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1375"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1403"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1427"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1458"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1489"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1894"/>
            <end position="1915"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1921"/>
            <end position="1941"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1953"/>
            <end position="1971"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1977"/>
            <end position="1993"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2000"/>
            <end position="2019"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2025"/>
            <end position="2046"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="16">
        <location>
            <begin position="1567"/>
            <end position="1702"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="16">
        <location>
            <begin position="2102"/>
            <end position="2233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8106752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2302" mass="266259" checksum="DD192D5B87DE41AE" modified="2022-10-12" version="1">MNYNHHQIVETWLRRFRECLNFCYGSDSQMITMELHKGNVQLLSVPQNKNIFNTRKYKLYNFLPKTLFNALERFGNIYLLGISLIMLIDPTLSPFYRWITIFPIGLSVIFYVLMEFILDIRRQSHDHKINMQTTSRGAKDGSIETIKWSDIQIGDILYLIKGDIVPADMILLDTGQVRDREAICMVDTQYYDGKSTLTKKKSSYLTQLIVLRTRLKNQFPEYRKMLTGKLEYEAPNGNTERFHGRLKLKKDPKNEELTIDNFIPKGTKIKQTSWLFGLVVYVGENTKTMQSSHYNAQKHSFEEKQCNFYSFLMACLSLFFTLISIIVLLARSDEGNFALLIDPNTTNGMKVFQLAILYAQLIPSTLYLLLDFVNFISLFKYEINQIEDNIIKYVKINSSNNLCDLGHVDYMLIDKTGTLTTSYYKLDNLLFGSLSFTLNYDQLQTTLNSKSVKPEENEDPIKFASINDNNEYLIPFEYDKKSSHTDVQPSGKLLLKSVKTSNNNPVFNQITALPQNKRMTMLHNLESSVQQPLNDFLQSRIFLPQPKTRNSPGNNNDEFIQLVNQLRNSSPQQEYIQTEDINTLYYDAFLKCLMLCHEARLVFGADSITYESFSKSEEIALTFARSCGYSLENFNKFDSPDMYLCKVRGNPIWYQVLGLNLFTYTRNLNSVVIQAPMTMDLELKQKHEAINQLCGEGSKNNSLLICKGDYEAIKSKLQLNHKEREELDSYIQHYKQRGIRMIIYATRVLSEKETESYKQQFNLLHSSLTNQDTLLEELALEYEKELNILGMTGFKEELKNDALDFIKTVKDCNIDIWLLSGDQEFQTISCAQSLEMMEASKYLRIVATDKEQIWLQINTAIGQIQSELQKLQEKQQDKHLNENITNRSMIKTSVTFFEGALYQQVLKFFLVVNGHSLSLISESPDLMSHFRFLSCVCKNLIGFNMNPQQKELACMIIRNYFPNNPTVLGVGDGYNDALMMQAANISIEIINSKRNHIYPQVNAGDISVNTLFEIKVLLLQKCKLHSERVSSMIIYLFYSAGFLGMTLFFFNWFCQFTATSLHDSMTVFLYIFLYTTPNALVIGLADKQTEPLVNARFPAFYIDGQIRTRRFWLNYLIEGFLESILCAAYTLYSCIYMVNYAWTNDGYQSDMQMVATSIIYILITVSSLKVLFRLICNNVSVVVIAFLFTFGLLVGFVFLNYRGDFSNYDYQELTYQLFTRFNSIVAMIFSLIGCYFINYFLHDVIKLLYFPNAYQQFAFKNKSGVEQELITNKKILNQCLDLQINVSSIIQKVFIDCSTTSPYIQEMLNPGDTKVTEMKLKPLTLEMKELVLEQKFLAHKLIQSLKHLRLFLCILLLYYIAYCLTDFFMTDQRVFTGAYYLVVFGIIFIIILFSCSHIMEQQYYTYSHLIVLIIFAIKVAIDWLSDDLTITLSATIVILFSTFNSMNMSVIPIMLYNICYLIQLIVRILIIVISTDLSTSNGTYSQSRVSVYAASTQILLVVSITIRFLFSYFKSIKHRRNDYLAKYQIEQDNVAAQDILSILVPRFVRQQISTGIYSMQQAQDDVSILFAYICDFDTIMKEEGKNVVLMLDSLFRLYDNLCIQHGVQKIETVGYTYMAATGIKACEQNMTAHVTRIEKTMRLVNLAFDMMQQVQGRKYGKGNQIEMKIGIHVGRVIAGVIGHHKPQFSLIGDPVNQTSRVGSTGDTGAITLSEQAFKQAKHGIKYYEKKQKEAKGLGIIDTYQVFKTKPIGYQIPKAFQLWQNCTKTVVKEVRQKGISKTKKQREFLSQLHNSIYQDNQKQSNKVEISPKGPQTMPMLIPQGSALEENKSRLTIPVQISEQVLTEDSLLITNEQEDRELELIKPNIILDIPENEIKSNFNQILKEQNIYESRVGMIFLWKTYFIITLLSIIVRKLFDHDLLIFVLRAIFLILVFVLYPILSKAYRNRVVNTMYYILIIYAIFTVLFQAYLTDNREVAIICLLEILYIMIVSCQMKMFSFLQVILYMMIMLALFLGFYISTDLITHYAIFYICCCMLILLLGYYLAMSEQIQMFNNLQINEEKKVKQINLVSQLLPMHSYLKMKNSSIYDKSDLIDEFDDVTLLFADIKGFTEYSHTQTPEGVVTMLRNLFTEFDKLCQRYNVYKMYTIGDCYVVMGFTNSAKRNPIQEAINTVKMGFQMVDIIMSVRQKIKFDKLNMRIGIHTGQVTGGIIGTDIVRYDIYGKDVSIANKMESSGVEGRVQVSETTKLMIERAEKHAFNFKFHHDVELNKFNMNIKGFLVDWDKRREDASIDPYRSPSRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q0M6</accession>
    <name>A0A8S1Q0M6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1380067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000142">
        <property type="protein sequence ID" value="CAD8108805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q0M6"/>
    <dbReference type="OMA" id="IAIDEWN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="223"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8108805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="804" mass="97570" checksum="703A156CFD83A1D9" modified="2022-10-12" version="1">MIQNQSELEGNNSMQAQKKISVKLQIRLDNANNEEQNDNQIIQNVFVYYQDQNEMSIDFNHSKMEIDNNQIWFANIEIDPNNVYKYFYKVQQKNKIKQESQTRYFKYDQNGRIAIDEWNSVWCLYRYQQEDPKILEYFQIKIKDYHKKSYQSIDHQNVNESGITYLECSETLKFQEQQKRQFAFIYKNNQDQVELSNQFDPRKGNSFMNNYVLNKFKESIILSNEQEQRINTLVSQKQNISNKNEEQYKKIIQNLENKYNMKQIEVNDYRLQLQKEIERIKESKNKIDQLEQRLKSLESENNHLIITIQENERLFNIKLKQQEYQREDQILKYLDNKNIEFQNKMEQVQKRNNEMLKKQQEKMEEDQKSLQKKSQEVINKYIQMLEEANQKIRNQELKSIQFESDDIKIQIDQSEIPEDYKQNIKDLEQTILQYKNTEQSLKKEKEEVEKYYNDKHEKQKSQYQLKTQDLLNKGVQCQQELNKCKQLLELKIKESEEKVQKHDQKVERYQIQLKVLQEKFKKKSDDLEILLQAFKELKEKLEKEQEKNYQLQQELEYTIKSSEQKRQEEFMNFRQAHDQEIKQLQQQNQQELLLEKQKHEQLQIEQQYQQKQMLNELMESTYQKISDVILDPYFDIQKKFHNNLILNFKSEIKTVIKENIDNFETPINQLSLKLSHIEEETLEELKLKALEKHDELVSTIQKFKDDSKIENAQILKESFSKYTAVCNQIQDMAMKINFNNDSDGEYIFQLTQWKSVLDQKLDSVFRLISFSNELQGWNQQFENSRDTITKAYFDLQIRHKSSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2R6</accession>
    <name>A0A8S1Q2R6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1410148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2R6"/>
    <dbReference type="OMA" id="NPCRSSQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="233" mass="27568" checksum="7AAA65985A7D27E4" modified="2022-10-12" version="1">MQSFIKNNPITGNAVSKIQNRGKRTFTAHDHIQSSCIVKSTPLNKTPNRHKIPDHSNEHFYPQDNSYQKRDLRKSLSTFQPKENIRDPLSRKISDIYGQEIKITRSPIKQQQQSETVSPQKRRIMDNKSSHVDQNDIYEVNLNYNKEKTLPSDVNWFYVDTIKHRHDYKIEQPNPCRSSQKDQRSVLDQNNKNVPQKNYYQKQNDKLSKKQEVALQYTYLRAVKRSELVIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q3S1</accession>
    <name>A0A8S1Q3S1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1400083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q3S1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8109318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="61" mass="6969" checksum="F5ED981B1D9E4F8F" modified="2022-10-12" version="1">MSIDHFFSLLGVKSLLPKGEAALFIAISRNALDTKYQSYAQHSIEIDCFSQSQYQMHILYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7Q3</accession>
    <name>A0A8S1Q7Q3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinase domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1480092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000152">
        <property type="protein sequence ID" value="CAD8111666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7Q3"/>
    <dbReference type="OMA" id="MQGISFC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="53977" checksum="294DBC52628946CC" modified="2022-10-12" version="1">MGNKSTREIREKIEQFKEDSEKKDIEWTIHNQDEVYEQMLILSRYFSLQKLQLNFIDPNIGDITPLKDAIQGSEIFEVLKIQAQNTKINDANIIPSHITTLNRFHLDLSNTQITDVSNVIQVLNQNIQLESIYMNFNNTHITSIKLFDVHLFTQLKSLTIFTGYTQLNNIEQFTLNVSKLMNLEELHLNFSGTQITDLAPLGSCIRKLNKLLQLTIKLDDLQLENIFDLMQGISFCINLCHLTLTFNNTNLNNIDQLGDLLKQLQNLEYLNIQFSKTRVTQADNLFQKIGYLTALKSLSLNFKDTNISSLSLHRILDTIQKIEHLRISANKNFDQSVLDAIPQQQQMKSLILDLNDTNINSVNKLMEQIGDLKLMRFLQILISNTQVEELNCSNLSKLQNLRTLQIDISQTPMQKIIGLEEELGKIKFLNQFIFDAIDTKFHNNEYQDKIKQELKKLQYLKEFHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q834</accession>
    <name>A0A8S1Q834_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1490003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004649">
        <property type="term" value="F:poly(ADP-ribose) glycohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990966">
        <property type="term" value="P:ATP generation from poly-ADP-D-ribose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007724">
        <property type="entry name" value="Poly_GlycHdrlase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837">
        <property type="entry name" value="POLY ADP-RIBOSE GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837:SF0">
        <property type="entry name" value="POLY(ADP-RIBOSE) GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="415" mass="49552" checksum="C405DEA8DCE79AF6" modified="2022-10-12" version="1">MFKSEFQCNLKDYPQQNYSEFIKGIIDSDQTLENIFNGDQGQFEEFKIYLKSKIFNDPNEIQQHLTNIYNQWNTQSKVVIKRIEIFRLIMLMYLGLLERNFSQGGFYIIDMSSIKYTNNYISKQKLKCFQNYIKTFYLNSDAIQNDQLIFTKNTVNKEEFKKKFEQSIYQNIDFEFTQLKNEDHQNSTVVDFADQNIGGLVLDTRNCAQEEVKXSLWLNKSQTIPIETGNQLQIEVMPQLRACTHELMKNYDKQQKKIQKYIAKLLWISQKQYIKRLNLCNKVNSFIFDLQMKNIHLQVRYKYQVPLSLIQDHIFFCSVLHVLITNLALLVQLDNSKRSQRVPIHPYSPVQQQLVKNNELITQKNRHHLLKFIKNYYNTMQFLNRITHHQVSSLTRQTDQLCDIQINFCDTQFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBS9</accession>
    <name>A0A8S1QBS9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1510058</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000156">
        <property type="protein sequence ID" value="CAD8112404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="CNLYTIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035933823" evidence="5">
        <location>
            <begin position="19"/>
            <end position="2487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2088"/>
            <end position="2113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2133"/>
            <end position="2149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2191"/>
            <end position="2212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2252"/>
            <end position="2277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2289"/>
            <end position="2312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2340"/>
            <end position="2358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2364"/>
            <end position="2381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2393"/>
            <end position="2411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2423"/>
            <end position="2448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8112404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2487" mass="283791" checksum="E1B0BAC3625F6ABD" modified="2022-10-12" version="1">MNIQMYLILLMIASSIYMRQISEYCVCRHVDNKDECQRSQICIWNGEKCDLRSGETYIYQVNQDDADQCYKYIQEDCIQMEICGFYLGECIGFTECGLFQKNQCQESSLKCVSDGQKCIEKRGCKDYDTEVGCQNKNINGNYCYWDVHAIFKCQDVMKCTQLPQYLLNDYDCRNAMYYCTVNDLGLGCEEGKNKCEEYKLQNQCYFTYNKKIECFWDVELQMCFTKNCENKRLKTYEECNSYMSQCTTNGIHCIEKQECKNIGNAIGCVLDINNKKCVYFNGQCQIKSCITAPQSYNNYQQCQSYDQLLDCVSAQNGGCKDRPLQCQDYYQEIDCYSVSTQDCVWFENKCDKRQCLHAPKNFDHYQCKQYGKCMGILQGGCELRPQTCEEIIKQEFCDITYDESKCIWEDNQCQQLICSNLKLPKYDSHQKCKQASKLCTFSIEVGGCIDYICKSVVDISACTIDSLDQLCNLNDGCIVKRCKLAPSYYDTNAQCELWLPTCTVNIYEFDGLKIKFGCTDKPLECSIAQQEQCYSTYFGVQCKWQLNKCSNQQCIDADNTHVTNEDCTNFKVETIQCIINSTNNGCIDWPISCSQMVTETQCNIGLKDSTICIWSSNSCRQVDCVDAPLYINNLQCNNYLNNCIVKPSFQGCMIRPSNLICSQSPNTKMYDTHQECQAWNPNCTVSDYLTSVGCQQKSASCSVYTTKNQCKIILDNSYPNPNPQSYCFWDYITSTCQSAYQNSSYQCNRQSFGELTHKNCEDFMKICTINNNITKSCVSLQDDCINYTIPQACVLNKYQQPCIWDKSQNKCQNVLCSENTVAQTEAECFKYQRQYLCQLIYNKEGIAQKGCEDRPTNCSDIKYQKICNKTVTKLNQKCYYYNNKCNIINNASQCQLITEASSDEECQYYFSLCLLQQSGKGCYSVDECKNLKNFNCNSSILFKNNKCFYYGNECRQNLFCDQLFPSQSSCNDVKTSLNILCSYQYVGSELKCVDQECALNNIIGSIVDKYSACYNYSSICAYDITTDSTTKSCMPIINCNDLTQQGLINYLLCNNSLTISKQKCGFNFVINQCENRQCEHLKFANVGLLSDEDCYNWNHDCIFDGTNCKLFNNIDCTQIKLKHQCIQYSQCKLNQNNCISNIDCEINTIAITMYECQLINIICQLDFITNQGCKYVQCEYITNPSQCDSAHTSDGMDCYWSSSCIPKSCTAFTNSSICQSKYGLLVVNSNNISVKCYWCSGSCSYNNTCNATIDLGLVKHEDCYNQNILNTITYSNGPICIQKQSSCAAYALQAFCVKTIDGQKCAWSSGSCKDYCMSISTSPLSHVDCHTFDNNCMKNGTTCVLLNCSSLNVTDCLIYSNICTENSSNQCVKITNCNVYNSTNCSTGQDFQGYPCEYVSGTCQKQSNLENCSSLTNQDDHQSCESYFQSGICTVKSKNTNCTNLPINCSQAAKKQCVIDQNKNRCYWKQTNECILLVNCLDLGNENDSHYKCQSYLSQCTVNSLLNGCIDLIDCNLYTIKEQCYKDNQKRDCEWIQLQNKCELKSCSTAQLQQYSSGACQQYYGLKCSVNADLTQCEIAQGQCLGYNQQQCKSDGQRNFDGIECFWNVEKGECVEKTCENGPKFALSDKECQGYMQNCQKGGCRLKVCTDYYYAIESACASIFSDKSCTTNGYQCINRNTCEEVLIKDGCTFNKFYNDCVWISNTCVTKTCNTAAINLITHKECQEYLPQCTTKKGGGCVLINECTDFQIKDACQSDKYNQGCIWDDQLELCFTDICEGFCGDGIITNSSEACDDGNYLPYDGCYKCQIQCSLGCLQCEGKLCNVCDSYGWKLVDGQCKSVCGDGIILGKEYCDDGNLIEFDGCYNCEYSCDSHCLKCFQGTCIQCPIGLYENQSYCLNKCGDGILATQYEQCEDANTDNNDGCNYNCQIEKFWKCYLVDSLSVCIYNISPSIKLTTPLNSYGQNQEVILSFSEEVKLNSTSITEEIFIQLINISISNLKDTDYTYEIVPILSVDYTLQPVSYKIKLNFYKSVIGPILIVNINCDQLVNQYENQLASNQQTILLKDPFTLSSTQKMVAANAALFNEFVIYSLISIACFAFISGNLEIFWNLIDTLQQLSYMKYLNLQFPQNLSIYFEVFTLVTIQPITDKIKLSGFFEEMFQFTSPYIPAYGRFEVFSINCYFGINLQSFVIILILGFINYIIAYLIQLLLTSFKYYNWPIIVDQRYLEQTTKIIKAIYFVQRICKSYYQYFLYSGIVRIYLSNFYDLMFSALLQVANFQSYDSITEISSFLALSILILNFAFIFQMYSYLSTRKTLSKRFSIFFEGLEIKENLWNKQFTTIVLIKKTIFIANLILIQTIPPVQPILTSLISGFYSIYVYRMRPFANKFENYKIIVTECLIAIIAILFSVSESMLLFSYIDYVSFLGWMEIAGFSIILLNSLLIDFYQQLGKPTRQIYEILKKCGNKTTLDNSNSTILKEGVIEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBW7</accession>
    <name>A0A8S1QBW7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T1540061</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBW7"/>
    <dbReference type="OMA" id="ARCIECQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000342">
        <property type="entry name" value="Histone deacetylase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF6">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="15">
        <location>
            <begin position="24"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="16">
        <location>
            <begin position="153"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8113152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="412" mass="46785" checksum="93AA3425C7C17BF5" modified="2022-10-12" version="1">MDQLDGFQIVLADGSVGFAVQPKEDCEHFKQTDLNELMKYVEQNRKNLFSQPCVQCGDCKENWVCQHCREIHCSRFVNSHMVEHNKKSGHQIVLSLTDLSFWCYDCSSYITNYLISKASKLLSSIKFNNQEDKNDETQEIKQLIEQISNLKVTNEDEFNYNILIDGLKNKKFQKVCVLAGAGMSVAAGIPDFRTPGTGLYSQIQKYNLPSPESVFEIEYFKKNPEAFYCVAKEFLLSFDAKPTVAHKFLKLLDSREQLLRCFTQNIDGLELDAGVTQEKVIQAHGHMRSARCIECQEGASIKDFMNHIKKGDIHRCEKCPKKGLIKPDVVFFGEGLPGEFFYNWNCLKDADLLIVIGTSLKVMPFAASVAKVGPSTPIVLINRENVLQGRKNLLHLDGDIEENCKKIVQDMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QFY4</accession>
    <name>A0A8S1QFY4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1620002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000169">
        <property type="protein sequence ID" value="CAD8114889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QFY4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="105" mass="11809" checksum="E208FA430953A382" modified="2022-10-12" version="1">MHALQLHQVPPLNQRPLVLLELMELIDGYLLENVRNLDSTKIEQKKGINDQCKLIYVIGGSCTTDRTKFIPQSTCSSYIEAGCFNGKARKRLFSLPVRTTTGNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QN69</accession>
    <name>A0A8S1QN69_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1690022</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000178">
        <property type="protein sequence ID" value="CAD8116085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QN69"/>
    <dbReference type="OMA" id="WCFEVDE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="27"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="459"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8116085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="534" mass="63058" checksum="015D5E1FD65CC272" modified="2022-10-12" version="1">MDNNQQQQIFEFYTFCKTYQLQTKRTFLQEELIGSGNEGQVFLAKPQDWCFNVNQVAIKIQQVHNEKIFEFYQKLSQQQLKLNQNESNEKSYIIKIYEICVTQQKNLVIIMEKGGQDLYKYFKENKEIDFKRKEQICLQLALAIKQLHQLDYVHRDIKPENFVIVNNEFKLIDFGLTKSIDEKQMTMNVGSRIFQAPEILYGDGNYTFSVDIWSLGCTFFEIFANQELIQAKTYLDALNIIVNHIKSQKYIYNRIETLNISQAWKDLIKKMFNHLPNERITSNEVVLTIQSFLNVINNKSTNNFLLQNNQRFQSPLFNPPFLNSQQNIPQINNNFPQQQNGVIQNNNAFLNINGKNNHFQGSNQQMQNKCNENQQFHPPKFLTPNLQNFPNVQSTKAQVQQNSQTNICLMTQQEQIPKQNGILNQHQYVLSTQQFPNQNNDQQMFFNNDTKFQVQEQITQQFNQRIQKLETDLQAARNEIKNLQDENNKLKNEKQLLSDENGEIKNQIRTYIQQQQSSIEVLHKYINQSQQQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QP49</accession>
    <name>A0A8S1QP49_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase S49 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1710019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000180">
        <property type="protein sequence ID" value="CAD8116327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QP49"/>
    <dbReference type="OMA" id="MNFRNRI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33209:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_55, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33209">
        <property type="entry name" value="PROTEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S49" evidence="1">
        <location>
            <begin position="69"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="219" mass="25178" checksum="0F1B04D3F6BB97F1" modified="2022-10-12" version="1">MNFRNRIFKIKISSIITPQLVEKVADSLDKWTIYKPEFIALIINSLGGSLIQTQNLAILIKKYSQIHKIPIYCFGEDIVIKSALGLLTIGDKAYVNPYAIVGNMGYAQPYFDLRKLASNWYIHQKHIATNDNMKLLNPLNELSNQDKEWVEQQMKNQEAELINMIEINRKLDKSTFDDRLKDIYHNGIIQPNVLLKHGIIDGYAGFDAMIQGKKVRKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QP56</accession>
    <name>A0A8S1QP56_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1910006</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000200">
        <property type="protein sequence ID" value="CAD8117202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QP56"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="255"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8117202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="48115" checksum="4AF55584947E9086" modified="2022-10-12" version="1">MSQIMREYMKKCSSEGHDFVQFACLNQSCNAMRIYCFQCIQNGDHFNCHKHLKNLDQLFEFFKQIEEESEKLIEKIWFLQESIRELFLQLTKKLRAEFHFSKSALLEINPKKLSEALDKVIKYDEFKTVFLEDVKKCSDDMILQLNRLISQFEPFQNQEIPQYNFNKTEQLNNSQVNKSNQQQIEKSYQQGKELLIKDINYFKRITTKKQLNLQIFHYSLIQTIQIHSESLRMCKDYDGAIIIADKVLKIDSNHANSLYTKADSLRLLGDYQNAIAFADKALLIDPKHTDSLYTKIESLRLSGDYKGAIIQADEFLLIDPNNTDFLYTKDNLIYLIQILDQALNCDPDHELCLASKGACLNNFNSLNSVHSQLKYQEAIIYYNKAIEINPQYSWAKNKRAECKYKLNTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQ68</accession>
    <name>A0A8S1QQ68_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1970003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000206">
        <property type="protein sequence ID" value="CAD8117372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQ68"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="209" mass="25539" checksum="3E9D21ADB0696C02" modified="2022-10-12" version="1">MEKKFQINDQEGYKKGHQEGDEERYQEGDDEKGHEEGDEEGHLQVDEEVNQEEYEEGHEEGNYSKSKEKEMNEEKTSILMNFENLQDKNGKVNEFYQMIKKQYYQLMGQLDQVKVRRLRQKNLLMKKSVMKSQYLFIFHNLLQKILCFKEFQEVEETLHQYEYDFDELQQKQCKEMLKKKEFDDILEKRINQNKSSFQNLIRHKQRNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQW9</accession>
    <name>A0A8S1QQW9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1860004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000195">
        <property type="protein sequence ID" value="CAD8117007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQW9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="87" mass="9874" checksum="8C3776EB15319086" modified="2022-10-12" version="1">MVCIKIGRKISKLTCQRDIKIEKQIESSDQNLSDINVKSLTLHQNNSFLDQVYSIVTILPISQYPTLQAKGAKIQNSEFIPHQGFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMA0</accession>
    <name>A0A8S1JMA0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0040567</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMA0"/>
    <dbReference type="OMA" id="HKLYMEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070525">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000201">
        <property type="entry name" value="TP53-regulating kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8043343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="212" mass="24493" checksum="AA138545EC2D899F" modified="2022-10-12" version="1">MQQLISQGAEARIYKIQFLGDLAILKERLSKQYRLKALDDRITTERVKTESRLLSKARQLGVNVPHLYYVDNNQIIMMYIDGIKLKDYLNNLEKENKANEIQQILTLVAQDIAKLHNGGIIHGDLTTSNILVQNSKPYFIDFGLSYTKVGFAEDYAVDLYVLEKAFLSTHPTLENEFQILLEEYKKLQTKGKEVDLKLREVRQRGRKKVAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNU7</accession>
    <name>A0A8S1JNU7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNU7"/>
    <dbReference type="OMA" id="PPRAQHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="114" mass="12962" checksum="AE37421DA6B98EC7" modified="2022-10-12" version="1">MIAPNFCKFKEVNQFTDLSNSQGSASRLKSSRVLKEHIYGSNLSWRQRETNSITKSNYFSIGEKTSQGVNTILSLVNIESAQFKRSSSQRRNKRNSESLSISFSSKMDTNRSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNV0</accession>
    <name>A0A8S1JNV0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase M16 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0050365</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="4">
        <location>
            <begin position="125"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="237"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8043711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="342" mass="39376" checksum="572B5A8F8B132180" modified="2022-10-12" version="1">MHYVPISQTERRISKLKHKNKQKTMFIIVGALLICLIAMLVLIVLDVNNYVKINSIFQIWQPQNTVAMVQTQRDVIQSKHKQIDEELFHLKSIFQEKAYKEATNLKLPIIDKNEYQYFTLSNGLKVLVIQDPEAKIAQAALCVNVGSWTEPDEYPGLAHFLEHMLFQGSKSYPQEGYFQKLVAEGGGSTNAYTRGEETNYYMKINNERCLLIFLLILYXIEINETYKDTPTSKHGDSQSNELDQTNNSIQQQEPEQKYKFNVIPLKVVTLTVFLFILGVIFVIFGLIFLIKNPKKIQQYLSLLIVGSLMIIPGLYYSCLLIKFIKADSRSVQEKILNELPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNW7</accession>
    <name>A0A8S1JNW7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aurora kinase</fullName>
            <ecNumber evidence="13">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0040292</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="13"/>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNW7"/>
    <dbReference type="OMA" id="KELQKCH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="128"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 11">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="208"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="259"/>
            <end position="260"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="10">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8043069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="405" mass="46732" checksum="7ED5F6484945971B" modified="2022-10-12" version="1">MQSDDNDWQKVVQTTTRIDQQIKNIKMRLQSALTQLIIQSEEQNKITNPDPIIQKTNPPLIIQHNSRSIEKKDKPALRQCASKSPIRTPDTAECPSPISHFSNNSTLRIQLKQAELLSPKYQNISDRFHIGKFLGKGKFSDVFQAQEKTSKVLVALKVIQKNVISKYKMEAQLAHEIKIQSYLNHPNILKLYGVFQEQTKVMIILILEYAPDGELYKLLKKQPNRRFTENKAGNYIAQIVEGISYMHKMKVIHRDIKPENILISLQFLKIADFGLATYSPESKPRQSFCGTIDYMCPEIASGQDYDHSVDLWSIGILAYELTTGTTPFYQSSKEDTMRKIIEGRVDFPKYVSNELQDFTKCCLRKDPQQRLRLEQMAIHKWIQMNHQAGGQYDRMLVQSLVTILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JP60</accession>
    <name>A0A8S1JP60_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cation-transporting P-type ATPase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0050090</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JP60"/>
    <dbReference type="OMA" id="FVNLVIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000162">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000087">
        <property type="entry name" value="Na+\K+ ATPase alpha subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000061">
        <property type="entry name" value="Potassium-transporting ATPase alpha chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000003">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000083">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000095">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF21">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="860"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="888"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="928"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1142"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="12">
        <location>
            <begin position="101"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8043437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1222" mass="137026" checksum="2B2B743631038499" modified="2022-10-12" version="1">MAQYRQLNEGDIPPINNPQIVTGFQILRQSVDLGLRQNQDARKSSVKAAALVSRQSQMYQQAKEKGVVLNETIVKYDAPSNNKQDLLKKAQNKGEEYRNMDEHKVDVIALSQRYETSLTDGLTQEQAAAKNQQYGDNKLTEKIKKPWWIKLILEMVQPFSILLWIASIMCFVLYGVNPNALGAKSNLWLAIILIAIILLTGSITYNQSAKADALMEGFKNFLPQRCIAIRGGEKVEVPAEKLVPGDIIEIKMGDKIPADVRIIQSREMKVDNSALTGECDPLLRVTELTSENPLETKNLAFFGTLCKEGSGKGLVIQIGDNTVMGQIADLATGGETPETPLNKELKRFVILISCIAVGLGILFLILSLVVEDASVDTAVGQAIGIIVANVPEGLLGCITVSLAITAKRLADKQVLVKNLEAVETLGSTSCICSDKTGTLTQNKMTVANIWYDGLKRVALNKLKHGRNTEYEYDTNDPTFRDLHDCAIITSEAKFNIQTKDKATVNWLETPTLGDASETALIKFFQPIEDIESTRQRRQLVELSDKSLAKMPFNSTNKFSLCIVNWETQDSYYCVYIKGAPEKLWTFSSYLLIEGRNQPIDEQITQKFKSVNVSFGKGGERVLGFAKLHLPRSEFQKGYKFNLNSIDTLRFKLEGFTFLGLLSLMDPPKETVPLAIKKCQSAGIKVIMVTGDQPPTAGAIAKQIGIITGKTVDDLLEENPMMSYDEAFRLAPAIVIHGDMIVQAIEEENQNGEFVPEEMKDRRLKSWCSKPQVVFARTSPAQKLMIVRACQYIGHVVGVTGDGVNDSPAIKQGDIGISMGISGSDVTKDAADMILLNDDFASIVDGVEEGRKIFDNLKKTIVYLLTSNITEVFPYVGEIAIGLPLPLSNAFILTICIGTDILPAISFAYEEAEIDIMTRKPRKKDDHLVSLRLITHAYLLQGIIATSAGFFSYFSTMNEYGFPPQLLLSFMNTSYQKIPWPSITLANGQPYTPPPTTWVWDLPNMNNPFLTTQPYSPDWQQAPILNDTNGYIGFQKTPLNWINPEIIYYDLRYIFVYYDSEYHRWFPTFKEWQNQNSDELCRYFDRSDSLLENNSYPINTNACFKTAALKYAQTSYFVAVVLVQWSNVFSCKQRKMSLIYSPINVVMFYGVLLETIIFICIVYIPGVNTWFGARPVDILNLGMPGLPYSMCLFCWEELRKYFIRNFAKPSKFEPNFFEANSLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQ59</accession>
    <name>A0A8S1JQ59_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0080229</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQ59"/>
    <dbReference type="OMA" id="ISHSFWT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF50">
        <property type="entry name" value="PEROXIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8044663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="231" mass="27423" checksum="3255C53123AF4A1D" modified="2022-10-12" version="1">MLQNWELLRSYMLLGIDNLESEQKFIIYQQIYVFRRLLRLGKSLQTILNILQKSQIHNPKKHFHQIPHAVNEMSTYSVYIFYFFFHLIENIMVIHQMGFFEKTFNHQLLKIISHSFWTLGLLTQLVFYLNRLRSNFRRESEMKQQISNGISNQEFISQIRALTNERYQFGLLILRIIGDLTCAMQKAQIPEKLLNTRFNRGLVALGGLMSSAIQIYLQATREDKKQNVCEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JS12</accession>
    <name>A0A8S1JS12_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0090397</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JS12"/>
    <dbReference type="OMA" id="AIAVQCA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF50">
        <property type="entry name" value="PEROXIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="281"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8045394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="345" mass="39997" checksum="81E201A51CAAB791" modified="2022-10-12" version="1">MKRSIASVKDLADYFNVAIQHRANNTIVAGQYVARKVSIVSLFMQTTKGRDKICCLIQYLADLYEACIKFSNIPEIQAASSDMFSKKIASRIRESMKNGRKIFKFLKFFDSIRCLSNIHAKNKPKYYKITTSIMHVCNFFYYIMDHIIWGINIGVLNEIVSLKAKSTIKGYKDSFSLAKALLKLLKSFFDYKIIYDKEMDLVKEVKEIPDKLIYEDAIAVQCANSLINSRQKRRIKQLNFIVTSLRIVMLLRRLKLFGLNKKISKIFYSCSGLTITIINIMVQLINNNNLINQKLEKNEKEKEKEKEKDKKLARVNSFIPQNHQLKKVKSELERVLGEDSENEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTK8</accession>
    <name>A0A8S1JTK8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTK8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="106" mass="12582" checksum="874DD8B3497BB62E" modified="2022-10-12" version="1">MLIDVNRINQSDSKVIQSKSQQRDKIRRSFDSKLTAYIESTSEQIARSCILRKSLSEQQDRNLTEKQSEMRREQEKIRQDNIRINQQIGIPKKIFLNLNICQILDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JUL8</accession>
    <name>A0A8S1JUL8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0060188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FKMISEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="1174"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1048"/>
            <end position="1075"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2771" mass="329159" checksum="F5D8179E446C6091" modified="2022-10-12" version="1">MLRGGGACTSQTKQQSLSISSLQISNQFENVISSNVLLLSKNAEDFNNNYTQLYNSLQILLSLDQELNNLTKSRNQLNKQVEYLEKWLTEVLESCLKLKNPQLRYLALQFAQKLSRTIFWYYCQAKSIKDRMQQQYISTLNKIESQNNALDSFYKSMIDYEIMVCKMIFVILPNDIQEGLEVMALFTSYNQILMTTLPKSTQDTIKSGVVQSIRDATKVQYRKIYKQIFNIDTMKWWVIQELRQNNHSAKEIILELHNFHKDISKSDYYVHYAWIQAISDIVSYAPIISEDQIQINNQDLQDLIDMGCLYKIKNDYRVTLNFIKIRNQHCQQIYEKIPYFTMIKQIQTQIISQDKFFNSLYKKKEEQKELQKQDQETKSVFEYIFQLFVEIDIEANIDLIKKVNQSYIELNENITLIKQLSLNKWIRMLEKKSYDDPVVFGEFEEKQKQIKHLINQVKGIQRFLIHFDFILLQWNIMEQIANSEGKQTQLQSIFLENQQNYLDCHLISKINLENLKDIQLLNINFDVYFSIITEYIEKQYQTVIKVVQTIRSTDPIISLYKQNKDVTDIVQSSLRKVNDKIIQFKNAFMRISLKLKQVDFNCIQQFYLVSLRLQYLEFPNQQFEFRNQDHKNLFYIQGYEKQIIKSQLIELHKEFRELFTSSQFQDYEQAKVYIRSIQFISKTIDNLLNQNKKIIQHIHNYKLSIQIDYAYDYYNDLINQWNTIFEMAEELDYQKYDQSFIQIKYDELKKNYDSICQINISIRWPLNPQLASYFKMQNQLEALIQELQIYQSYNLNQDLNSQFRYQSIITNIKQICLSQVQKVKSILEQYTQGVDQLSNRLENYTNFQIEELQNLLTSVQEALDDTLNVVQSRILTSNQTSTSGSINILQSNKKQDFETFYNYKFNTETIIVMQFIKFNFNFIDQNAILEILKSNQNDLENLHVVQSAMESPIIRQIMIVNLINVWNCCLVEEDFCTISKLILQIRLNETNILIKREIKSTYKKELNQIVNNQLQSELPFFELDLRQMIQERNSLYFQIKYEPTFELSQKLEEKINKLTNQIQTRIQNCQELEQEYHINIPFCNQLLSEFEKSYQGSQSLEIQEISFYQGKSITELFLLRMDNISRKIKISQVKNTYVEFECQNKNQIRSLFSQKEKRNSTIDRFLDDLQQDQLLLLQGESGSGKSLAIKMIEEYVWKRNDPIKIIPIIVKISQLKDPINNAIIETLRSLNYKLNASQIEQLQTHIKSNKMQMLFIFQGYDKLNQNQKCINFLKSNNLIQWNTPCLQNTPKYIIVTRSEQFKNPNHLRWIDQNELNYKIKNYWNLKIIPFDFDQINQFLIQYQEISIRNVVMDFYQLMCEFPHQDLLIYNFISFWKKINFNFNEQSLGNILLSQPIINKLIITLQQDTNIKNYKGSITQQLSSQLGQLKSSYYYSKMLETLQFEQPIDNPQILKVFINALPKLMQNLIDIPKIKNQFFNVFFCQNYQSICDISEANNELEKHWNQLINSQFFKMYTIDYSIAKAKLTINQLFQNDKTLINKIIKAFEKIQITHYDLYEEYMNYYFDKSVINSELQEQGIDNITFREEQWNFCLNLAQQLAFNEIYSIKLNNQSSSKAIGSVTDLQIILHDDIYSIKDTLITKKYQRNSLPLIEQFSVYSFESKIIQEFLVAKVIIKQIESIDVIPINELFDNSILKIKNLSNPFYIGTVNFIIQKLKSNFYSKKNLMKMIQLSSNNPDFCQLSSNAFFLLIQLDRIIRGANLMNIKLKDIQIEDCYLQDCNLNGCEWNNISIKNLTLTNNNMQNMICTNLKLASYFIRNNLKDHLFVRIFQSGKLFIIYKNAIKDVYGNLIINGNFEKCQIYKIGNDFYITTYNQFYLLKEYNNQIKQYQFNIGNVQSVTQFDQYIMLLQLITSSDAKIFQYNPIKQEIQFQIPILVQDIIKYNIKQFYEMQGNLIVFLTQTQLVQSMHYQIEEGQLSFDNLTVHTTLDNIECINQIKYYQDLDITVVTKQTIDTNKEYDIIYGTFNQLIGKFIIIENQKNQFLYLNNTNLQLISQETFKMISECELNENTQHLQKNQCYLIDNDTMLLTFQDSFLILSLQPFEVIKQIKIRTNDLIINQELNNIQLLTKSELLCIDIPYIQDRQDKIINSAFQLDMVYFVQNSFNFLAKMSKSSDIYIGMLKNNNFEFQNLCQSDYPQFYLNNDENYAIINKNNETVCYDLKSNTKMNYVALLYDRILWIKDNQNFFYLQHVYLEEFRNSLITVKNKIINKQSYRQIKSVQSTEKRLFITTTTNETIVLNQQFQQEYITTKQMIKKYREFYITNHDLRYESNIRNTIRIIYDSKNQIKDFWIFSETILIREQEDNNEMNTLVLLRKSDNLILTKIQIQIDHIVYTDNVIQIISQKILWLTYSAKDFQQLQSNILNINSIQISSNNLDQIAIVSTFSEITIWNTITYQIQQSLKNIELIQYFKMIPNKDRQFYVADRDVLKFYNQNQVRFTIQLIKKRIQIVTNTWGAIHHIYQVEEDKITLNSMNEDCKIINWKKVVFELDSIREQIDCIDNQDQKLIFFSGQKLQQSNFDSFDVIKSLTFPDSFKAIQIKYNQDCSLIILRSNEKIIIVEAQGLKIMNQITFNEIFLSFFDYASQLEIYVQGQNNLIFKQVKDMQNLIYTYSLTSKKLDLINISNDELMIGSNSQGLIIINKQTGSIELYNPIIIETKFNSISYIKTIEHNVSMLAKCQLYGNQLQNCNITLNNQTNISDIWRNIISDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JWB5</accession>
    <name>A0A8S1JWB5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0100412</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JWB5"/>
    <dbReference type="OMA" id="NCYILME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="10"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8046517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="592" mass="69555" checksum="12D741C5A859D379" modified="2022-10-12" version="1">MLIGKQIGDYKLLEELGRGSFGCVYKCQNMYDQSYHAIKIIQFESLKNSEGIVGELLKDEISVLAKIDSPNVLKLEHYFQSSTNCYILMEYCNAGDLEKYWEKQGRRIKEQNVIDMIIQILNGLYELHKHNVIHRDIKLANILMHNDQVKIADLGFCKQLQNKDMEVRLCLGTMGTMAPEVARFDSYGLQSDIFSIGCIFYQLLFGELPFECYDVQFYLQSIRQQKIDFQKYGVVISNEVKEIISKMLIEDPKERLTFPQLFQYSLFKKVQNMSQVSQVAQRNFSKIETSNFYQNIYDNKKDIQIQQTGMNVLRQNEDILRTQQNEQYQIVNNIQQNSRINQNHYDQENVQKLTYELLSTKYLSPNQQSIANTLQQLKDSEIAFKSDKQQNNISSQISDSVSLQNTQNIVKYQILLDALEYCQRTYTEIDQITFLIQSQSLIGKFMLLKRIRSESKVYFQELSNNPQISELKDVFKPFIENNQFDIVLNQIKSSKQILNNLSQNYDNEIQEQATGIFHLNYYQALMDLHTQIKLEINRESEIKKKKVLVIVLIHIQRCMHFQDLCSQKIDLVEEFFNIKNKNLIDIIKKVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZA4</accession>
    <name>A0A8S1JZA4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mei2-like C-terminal RNA recognition motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0120288</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZA4"/>
    <dbReference type="OMA" id="YNLEICE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007201">
        <property type="entry name" value="Mei2-like_Rrm_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04059">
        <property type="entry name" value="RRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mei2-like C-terminal RNA recognition motif" evidence="2">
        <location>
            <begin position="121"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8048028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="217" mass="25398" checksum="B6E51D3E64BAA589" modified="2022-10-12" version="1">MICNIIDIALSSTPQKGRTSQANTTVSSASSSDSSFESKYMPKLQIPRPQLSQEEKFEASLCAFDFKLLMPDEGSLQNQKLLFQELIEMKFLFDCDEKVLYQNTLYYNLEICEENILYDERTTLMLKNIPKYMRPGDLKNLLNKDFKSQFDFLYLPTDNNNEGNLGYAFVNFISPEIVLKFFKKYNNNKWSINDKQICQLKYAKLQGRRDLMNQMDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0U7</accession>
    <name>A0A8S1K0U7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100517</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0U7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8046620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="97" mass="11582" checksum="54FCED1F8A181C53" modified="2022-10-12" version="1">MLSQLEKPLIPQPQQFKLDIDSEQAHCIHIDINILLFSDNQIDQIGHMNQYILQKQQLFQFCKIEKFSMNVQILVHLNKVTQKFVVEQQKKMYNPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K478</accession>
    <name>A0A8S1K478_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K478"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035924802" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="20915" checksum="CAFA33DE13E3CFBD" modified="2022-10-12" version="1">MFFVFLIAKIYLIKSQNCYPFDEKIILGDYYYSKIEGQTMVILQYKSYEECQIYSQINKLKLRFESIFIENLIYVYTTNIYQNQSLQFNKDYNIKLGQKSLILRLPLKNGNQPFSILNHFRDLQILIQNSSLRFETQKLDVSSVQIESIALIIINYCNISQIIQHLQINLDFCWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K832</accession>
    <name>A0A8S1K832_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0180238</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K832"/>
    <dbReference type="OMA" id="KGTNTET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="309"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="450"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="809"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="845"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="602"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="103"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="207"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="328"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="450"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="532"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="546"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="816"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="847"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8051620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="963" mass="114808" checksum="C39A3175C54920C6" modified="2022-10-12" version="1">MTEAKSKRDLIWEEKRRQREERLLRMEGQMHDNNENQFQLPPPRLDDQVPISFPSQSQEYEPYIPPEPYVPIEMNRNPDPPRLYQGSIPQQSDFREQRDKRSNSQFQQQSAISQSPLKNPIKQQQDDYREYLKKQMEEKETEKKRRRDGTNNNQQQIQQVQFQQQSINPAPLPAQPINRQKQEYGEFLRQQMMEKEEQKKRRIQSGNNQNNQQQQQQYQQQQMLQYQVQQQPQQYQQQVYQPQQFEINQGYSQGNQQSIIPYQQQQPQQIDQNKQQKQEYGEYLRMQMQLKEQEKNDRKKNLQQRMETGDAFPFGKGGGGAPYQRGANNQIPQNYNQQQQYQPQSYNQQQPNQQPNYQQQPQYIQQQQNFQQPIQQIQYQQSIVTPQQYQYQEQNLPSGFDQQPILMEPQVDPQQKGGRNRLMLENQNIDDIKKKELQKLEMQRVLQEQIEEKKRKKEQERLQKEMEDRQEEERFRRQKEQEEMEKKREEDSKKQQQGKFEQDNIDLKEKKRLERIQERKNRKNQQQEEDQPQQNFQVYEQQQQFQPPPSRNPPPSQPKSENYLKEFLMNEKMKEGVSPHQSILDFYRNGPQPINKAEIQQNREIDDIKRQMLQQQENLQQQIQQVMSQAQMAIEGRNKAEQELISLKEQVKNKYLQEERHHDDLMIALVKNDPKWEKQVFPPHLLAQAEVGAYKDLSMPALNENFNFPNLNQNRAAPQFAPAYESKPLVLKEESKEQKQYFREQFGLQSLVSDSNMVPIDHNASLVPQMQPLNKKVSEIQLDKIDELNAHFEDSLKLGIGENYQIRNDNFKKRPPSNNGQNQSWNVPSIDSEFVKEIDDLLKYKPAQQTLNRPPSGFNKNGYEQRQLQQSTTNRPPSQTNQRPPSAFGRAALGVLGSADLNYKGITLDSEFPDMTMLKKGTNTETFDIAGIHKRMDQKLMHLNEIEEDEDLQKLDKLLYQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB66</accession>
    <name>A0A8S1KB66_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0180354</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB66"/>
    <dbReference type="OMA" id="DIDSTCY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="347"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="454"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="663"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="663"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8051831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="946" mass="112188" checksum="22A249567312836C" modified="2022-10-12" version="1">MINRSPTIKSINKQNQSQDPLLILTPPSSLREISCSFRGHLNRYVSIQSRRNKQEQQEDQQQQRQFRLHLQKLYQESFIKQLLDKAKPVSKMNNYQKQVLDDLQFGQDTQIDDIDSTCYKFMFNYFNNYPIIQPQSSLKLCSDIVFIINTLIIFIWLPFKYSFEFNSILQILDYDYYYIIEYLLIGMLLFEIIISFNQAYIFKGKVINQHYHIIYNYLKQDMIKDLICLISLIILLFQTLEVAELNLLLLCIFGVLQGAKLLKVVNNTQEYFNIQQNNLIPLVINLIAVIYVIHLVSCIWYTLIKDINNLKSLENVIQQYIQCFHWASVYLTSSGLSNLESSNNEQLIFSSLISIISILINGFVTIKIGFFLYNHSQRIKHNQYINMMTNYMNLNSINLNLQLRIRSYLQYIYKQEQQMNDEDITQIMGKLSLQLRSELKHQLQANILEQCKMISSNFSFKLRKQLVYYMEEVKTIPEQRILTLDEQDDSSIYFINKGEVDIIFEQTNNMNEKHSRNHIKTLQKGEYFGFLGFITANRRTATIISKGFCQLYKIQRKHFINLLEQFPDDKEKFYMIKDKVQFQQDLSMLDVRCYSCKSTSHLVNQCPFLHYQPDKDRVIKQQLYPISQEREDIQRKSKYINAMLQQTLIQDLAKELMKDLENVEESENLSEGESEDIQTRTIKSTSNVQTKSLTKSFSQYSNQLSKFSHRPSQTLTAGLSQNYPSSKSQEEIGRFEQEILKKMSIHRKSQQKETAGFGAKFESQMHNPIQENDEEIPIIDIIDEELQLQQQEIQRPAQQFYYSDKSPRIYRRVSQKVTFDQNLFGMRNRRSSLIYENYIQQLYEEEHYQIEFPIITQPSVGDFDKICEFREYYPEYNISRVMKYLQRVTSIYHRQNINSLYSFLFIAIQKGQAMKDKLSHFQSRGNIKPCSNKRIYKKGKVKTLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KBT5</accession>
    <name>A0A8S1KBT5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pre-mRNA-splicing factor CWC26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0140387</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KBT5"/>
    <dbReference type="OMA" id="YFWDGID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070274">
        <property type="term" value="C:RES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018609">
        <property type="entry name" value="Bud13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051112">
        <property type="entry name" value="CWC26_splicing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31809">
        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31809:SF0">
        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09736">
        <property type="entry name" value="Bud13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8050232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="33889" checksum="AC8A538934B07072" modified="2022-10-12" version="1">MDKLSYLKKYMDNGQSKTKVTYQNNIKDQDNIDVLMGRVISKENYNELEQQPIFVETPESILIDKQKKQLFLKQMMNKERSRSNSQGSSSSESDSDSESDNSSSKSDSDMSVERAPKPQEVIHRDVKTGQRITQSQRVEQEKQLNPRLKAKEDKQAILQANKQQIEMWSKGLVQIQEKQKKQEDAQQALVTKNEEQIKQIDMQLMGQQKFDDPMRKMIQEEDDKQITLKQSDFLLKCKFPPPINRFNILPGFRWDGVDRSNGYEMKLLNSINDNKYKKLEKQLENMRDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJ75</accession>
    <name>A0A8S1KJ75_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0230227</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJ75"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="477"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8055530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="868" mass="102424" checksum="74EF5C19C0E1B115" modified="2022-10-12" version="1">MKPLSQISIKEQSEYHFSGPKVMLDDHRLSNKLQRQYSDDLCKGSLYIQSNNYVDVVCTPNAINTARNNRVTKRMLTRRKKEENNQKLVDNQVFKTLAKDRLVKQFKRNLFIKSYVMSQEYKGVIQNHFKYEQNSSKRVEFNSEQQDKQIFLIPGSKFTLILDLLSLIIKIICLWMSPLIVSFSLDDDIFKWFQIGIMFQIIIEVIIQTNRPINIQGETITNQRRILSNYIQYFLIEDIIDLSCWVIQFFNINNLINSLICGIIILVSLKKLTKNYSNCIETMFLKGSFNYAIDLFTLIITILTFVHLMGCIMYYVGYLTMFTGQSWLLKYSIADSSFWIQYNYSIYWAITTMVTVGYGDITATNQYEMMFVNFMMLLSSGMFAYSINSIGMILKSNYDIQQKFKRSLILINNYMKKGQLSQPVQNRVRNYLKYYLLTENNENFDEIKEIITHLPTKLKQELTLGIQMSIMSKISVLNHNFSSQILNQLSQKMEQIKCIPDEIIYNNGDQDDQYLYYLQDGEVYLCEDRSNKLLQIINTGESFGEYQFFTGFKTQTQAISHGHSTLYRIHRNSFIKLFTQISNKDFQRFHNIKDSIIFLNNYQVILKKCNFCNLYNHANIECPLITYQPNKFKIVMQPDNNINQVMLRMKHQRVGDKINSLLIHNKVIESVQDYVQTRSITEFNDPQNRIMNIHQQQTGQINNQQIQSRQSKQITQEINQSKFNLINISIKKAQKQSLQGTQNLLKKKTQQIKNKHRYQSSLQNFSLKKQNSVYLSTSSQFLTINELDEDNQENQEQLDYLYAHPQQINLCIDKHYNYQGYMQNDNIFFVIKKLDKHKMKNSRIWNLQNKSQDGGIYVQDEYSIIIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KKQ6</accession>
    <name>A0A8S1KKQ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KKQ6"/>
    <dbReference type="OMA" id="ENRFISD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="255" mass="29947" checksum="2C39AEE71FDEFE8A" modified="2022-10-12" version="1">MGILHTSCVRECTNRDQNSNQELQVIQNKQIQKIQHHLTNEYETSKTTLKGTSQLSDTEKFMEIKEEDQSSNLKINPVPAFVLNKQLHQFIVPKRENRFIQDIEYEVKQKIQQIQNKEKFKALQLKYSKINDQQQPQKFDKCSSNVLNYINKQHDSLKNEYKIENHAQNESKSNLNYLQSSSLVIEKLQSTQLECISAPNKEIRGILKSDSLKKQSNSVDVKSVNSNKRVHFSEDIKIDSEDLLYKFKWRNFRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KQ99</accession>
    <name>A0A8S1KQ99_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0260145</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KQ99"/>
    <dbReference type="OMA" id="YIQENQY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000126">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000135">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364:SF27">
        <property type="entry name" value="SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="THIOSULFATE SULFERTANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="71"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="174"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8057640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="276" mass="32509" checksum="F7F39B8406344BC3" modified="2022-10-12" version="1">MRGSLISAPYLHYLVKNKSKIKIVNVIMTDHHHNKEENNKFVHAHRITLDQSTLNLIDITATPVPVPRKLPTQKTFEKYIQENQYDHLTPIVFFDEHIHFAARAWFTFRYFGFKRVYVLDGGFNKWHHQHDFDKNDSKVYEDLNLQDQPKDQVTSLTTKDVSVISYLKQHKDTKGDDWLLLDARDPPRFKKGSIPGSYNLPYSEYLNDDHTMKSTENLQKLYKNHQIDITKKIVNTCQTGKLSCIALLAQEILKKKELLLFDGSYEEWNHEHPMKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KVW9</accession>
    <name>A0A8S1KVW9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0270278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KVW9"/>
    <dbReference type="OMA" id="TICVKFQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF5">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037188">
        <property type="entry name" value="U6_snRNA_Lsm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="2">
        <location>
            <begin position="7"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8058551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="83" mass="9390" checksum="B7A182A1AE3138DB" modified="2022-10-12" version="1">MNQLPKESIIKLQSHINKEIVIQFQGGRQVKGILLSFDIQLNLVMDEVRELPSQRILGLVICRGALINSFAIDGMVEIENPYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KX57</accession>
    <name>A0A8S1KX57_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0290335</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8060060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KX57"/>
    <dbReference type="OMA" id="NHFKQPD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="19"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="346"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="365"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8060060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="548" mass="63920" checksum="ECE19451B8293332" modified="2022-10-12" version="1">MKDFIKIDSIQSIRLVQVLYDIALILKGSFGVVFLCQDLQTKEYVAMKVEKEDINSMSLEREIQMLDELKKLPGIPKIIWAGNEHNHNCLVMQLLGRDLSYHLKALKRFSLKCVVNISLQIIQIIEGVHRKGIIHRDMKPENVMTGKDQESNQIFLADFGIAKFYREPDGTHVPFKDNKSFVGTTRYASIGAHRGFELSRKDDLESIGYMIIYMYKGTLPWQTYHNVAEKEKTKMVGQIKQQTQIEELCKDCPQEFYQYFQYVKQLQYKSTPDYRYLHSLFEQLSITHGFKHDKRLDWTEYSQGTTKVSGEQTQQDTRQPARSYRQDDKIKNVESQRSDINNLQKAINNNRNRSRHQSEQCVNKSNNSGNSLSSSQINSQQSSILLNYQNSQFSQNNIRIGSSKLLSKLQERGIINLHQDSKGSLIQQQSSKSFHERHMSSHLQQQFITQQQQSFHNNSQVMQLQQEDFQDLEELEERLQKDKKSNQRNQVHKSSKPLKVKEIQNHFKQPDNQTDLQGMTQLEDASIENKLINYTLNAPKLKQNLAKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXL6</accession>
    <name>A0A8S1KXL6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXL6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="235" mass="26328" checksum="7C9E52165D2E50C4" modified="2022-10-12" version="1">MPIYPTAPLTSSIPAPLSVVQPVSYAQPVTYAQPVQYVPQPVQYVPQPVVQQPVVAQPILTQSVVAAPQQAPIKGESRVEYREFQRPVVEMETETIQVQVPKTKYVTDYYPVEYQTEYIPRTVYEQQTEYVPVTKTVPRVEYDAVEREVQRVQYQPVQTVPVQPVVQSFVQPVQPLTYSVARPIAQAPVYAQPVVAPALTFSGVRPAYAPVYPSYPQVGARPQQPQQVVPSNKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2B4</accession>
    <name>A0A8S1L2B4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0280022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2B4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="99" mass="11526" checksum="E43A1A2642110BD1" modified="2022-10-12" version="1">MNDTIVKEIMPTKISDKEKKIIEYLEEELLLEQFTDPLLKQMNFELSKKKTIFSFRSMDSDTEISEATSCSTIKSSKLQMCKSPLFRRRLSALVLEQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9U3</accession>
    <name>A0A8S1L9U3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0340130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9U3"/>
    <dbReference type="OMA" id="NCHKLIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003923">
        <property type="entry name" value="TAF10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03540">
        <property type="entry name" value="TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="103" mass="12244" checksum="AC1FB0D0EB6C48F1" modified="2022-10-12" version="1">MSEFLIQEILEASGFQTNDQNCYKLIAHLTDKWMIEMTDSMVQKLIQDRIRKKEKDPDEKLQLTTNDIVNELDQRGLKLHNPYQYPEIDAHNLQKVKNDIQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LA13</accession>
    <name>A0A8S1LA13_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0350231</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LA13"/>
    <dbReference type="OMA" id="ENYFEQC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="448"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="691"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="700"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8063969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="840" mass="99486" checksum="CE803A72CF06A156" modified="2022-10-12" version="1">MMIQDEMKNEFLILSPSQSYDGSIMLNNNQKENSFQVGKRINMSHPRQPSYEVEEDKTPAFETFDLKANKQTKGIKWQFRNQREQISAKINNTEVNTFQQNQKKVLAFINKLKQHLALRNKQFKIINSMLFSPLNIGKKVYKNSLQPLLNIIWDSIIFFFCILLLIVSPIEYIFQANGYGNYLYAILIILIILDQIYKYTTQICQEEYLQQSKQFLEYIFKSSKILMDTSTIVLLTVLILSENELIKTISLLLIEFIIYKKIILAFNKRLFYELSDLIIQIIQILLTCHFFTCINLRQEFIINQEVKEDQEMFEEYLVIYLNYILQFGNLKFNVEIDLIQNRIFNICTTFILLFYKILILKLIILDSLDFYQEFKKIKDLKLLQRFLYKQHISSTLKLQISQKLDEICLKDKKIQNIENYFEQCLEKEQIYQEFKEQQLINFVKQFSIFSKFSKTSQQQIAQNLTPIILNVNDKLICNQYGDQYNIYLLNQGKVAYGLTESIPEYQKIFSGQCFGQYSFFTGQENKNLITGLDYCLLYKLSREKFLKIISSNVQDLEIATFIKDQILFNNNYQMIDSVCLYCQEKTHLIINCPKIHLIKNNIDFYDKYLYSANQTRSSYNKRRKRNQVNFGNHFKQLNEIHSNQVSNEEISSEFKQLSSSILEQSQEKNDQKYPGESQNNFLNDLNNEIMKQSDQRDQQQQECFSQSSSQQQQQKLQSKNIFDLKISNILNRVHSFKLLPSLQGNPSPIVKLQTPSLPTVTEFLNLVSENPCMEDIYKVNLDRMQDFDTYYPEFNISMVLFRMKRNQRRNQEQKSKFTINQSAAITIGKIIQRSDKNVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LD57</accession>
    <name>A0A8S1LD57_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myb-like DNA-binding domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0360059</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LD57"/>
    <dbReference type="OMA" id="GMKKRMT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000010">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH myb-type" evidence="5">
        <location>
            <begin position="9"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="9"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="61"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="5">
        <location>
            <begin position="65"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8064305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="29172" checksum="7C3E97E234954191" modified="2022-10-12" version="1">MKQNKQNIHKVIRKRPWTDEEDLRVIELVQEFGPQKWTQIAQQLDGRIGKQCRERWHNHLNPLIKKTPWEEDEEWILFLYHKALSNKWAEIAKHVNGRTDNSIKNHWNSGMKKRMTEFQMKLGQIKQKFQREGIQILNDYDPLQKKALEIILLNKQYKNITSESSESDDISYPTKQSKKNRLDKELIDLQGEQQLKGQNDQMIEQNYISDEEQELSNSVNSFQLLTTSKKKSKIEYEENSIQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDT2</accession>
    <name>A0A8S1LDT2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein BFR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0370062</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AATF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDT2"/>
    <dbReference type="OMA" id="INFMAPN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025160">
        <property type="entry name" value="AATF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039223">
        <property type="entry name" value="AATF/Bfr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012617">
        <property type="entry name" value="AATF_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565">
        <property type="entry name" value="AATF PROTEIN APOPTOSIS ANTAGONIZING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565:SF0">
        <property type="entry name" value="PROTEIN AATF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13339">
        <property type="entry name" value="AATF-Che1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08164">
        <property type="entry name" value="TRAUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AATF leucine zipper-containing" evidence="4">
        <location>
            <begin position="149"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Apoptosis-antagonizing transcription factor C-terminal" evidence="3">
        <location>
            <begin position="333"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="48"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8065029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="52075" checksum="7AB8D8B4DB87A97E" modified="2022-10-12" version="1">MSKIKQKKKAMIKHSIDPENDQEDQLSYDSIDDEQYLNLLQGHVPKSHKVDINLGQKYQGKKTNNQQQHSQSEDDLQNESINSDEIPEFIEREQEESDDQEQQRKIVKQKPKKKQNLSTFQKELQQIEKEDLEMIEKQKENKKELKEVAEQIKEQQQIWGNLVEIREQMQKTLDAAKRLPIQKDAFVTEIQKSKDSKLNDLNCQLLNNITNLFNLYSSLYQIDQINVNDDGYDLNTQKKINNNFEDSIKVVEDDIIRWSSKSALLSSINKDSKSQMGFLLLTPIGQSKKALQNIDKLRQKTQLKRQIFRILGEEGSDLNETNNKHIFDDTDFYLDLLKETISFNNQNPSEEALKKETEQFILKRQAQRQEKENKVVDRKASKNRKIRFEPHQKIINFTSRIEPPIETISREEIIKNLFGLTSTIIEPQIQKKRKQSIHDVNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDV3</accession>
    <name>A0A8S1LDV3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0380054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDV3"/>
    <dbReference type="OMA" id="KRTDDQT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="203" mass="23584" checksum="BEAD2393C7A13ADF" modified="2022-10-12" version="1">MQAKGDTTIDMEMMMESTEHLNNLKSDSLKDQITDQSGENQSLNQKISQIDLEFQKLLCQLIESKLSQNTISQVHDLLKRLSSSLILEIPKGPQILNSLYQIILVRKSTIKRNFPFSPLSSVLSRIKKLIKKVKQQILKRLNLFQKSKDHQINHLSSFHLYKSQCKRTDDQTESLTRQLSFEQYKEPIYKQITIIQGQGLIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDX3</accession>
    <name>A0A8S1LDX3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0380070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8065777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDX3"/>
    <dbReference type="OMA" id="VFYETQQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="71"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="267"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8065777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="587" mass="67791" checksum="0755D101D4BD68E3" modified="2022-10-12" version="1">MKAAVEKVKGNLSTHRLTIKNIVQEVLKKSDTDFEDDEDLKNIVTLLQKDPNQRTQLDVDKIRLSFFRFKFFSELEQSMGAEMVQGLYKQLGYELQKKRQIIFNIGDIGKKFYIILKGSVWVLVQKKGLQEGSGPTEEDQKQEEQLKNEKNKEMMENMKKSTRKKTKKSKHQAFVTQVTLDDIFATMTDQEYLDTQFPTLTKVGIINSGESFGEIALTKQVPRTATIVAAEDTHFATVTRDQFNKLLSVFYETQQKLNIGFLSKVAIFSDWNEQMLNQLYYHFKLEERKLFQVIYKEDEDANNIYLLKQGEIELSRSVDVTQQSITPNLTLQKFFTKTERKIERVRTSVITAGQIFGHEEVLAGTKRQYRAMSISQKVVYFVLDKQRFLQYFQRGQAIQKLQKFDAKKLNQRTQSLDIIKELKKIPLLIEHRDVPFIDAAQTKTRIKNSVERIGNPVEHTGYEMLQGHGHINKAHYNFRRNIDIIKQNVQNSEPLSLDKALFHIEASKGSQISIINKVFPAAARPKYSIPECSISSQTIIKSLKLPRILTEVDSVMLNRTNKEYLNSFKASSICSIGLPDESQKTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LE35</accession>
    <name>A0A8S1LE35_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein eL24-related N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0370112</name>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0530103</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LE35"/>
    <dbReference type="OMA" id="YKFYSGH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL24-related N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="95"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="95"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8065129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8074693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="159" mass="18533" checksum="98AC57E3AB9901E7" modified="2022-10-12" version="1">MVIKTDLCSFSEWRIYPGHGRRFVAKDGRLFYYLNQKSRAFSARKIKSQEIQWTVAWRRLNKKIKTDEGAKKRRIRNLKVQRAIVGISLEEIRRRRKEDDKTRKAQAEQAAREIKDRKQKQIEASKKKPAAAQKVAQKAEVKAQQKAQKGAAKANKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LEB5</accession>
    <name>A0A8S1LEB5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0390073</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LEB5"/>
    <dbReference type="OMA" id="SPLMYFE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="3">
        <location>
            <begin position="150"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="246"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="284"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="392"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="442"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8066408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="508" mass="59377" checksum="6586CFCFC635BAFD" modified="2022-10-12" version="1">MNYQQAIQSCSPTKQKQEEISSPITISLQSEFEQLTLDTSPKLDPLTNQHSGILDFIDEIDQDEKFKKHQSLSLQKDLLRRNYKNKINEGQSSPHSNQSLNDQKFKYIKELWLQESPIQEMNKQVLSQRVISINEEQYFKDLQSDNVIGMGVYHSNEASPQMYFEPDIEADIYISSLIDSLWGNQIISRKLQKLIESGTPEQQLLIVQKLERISPQIEKDVFGNYVVQKIFDSCGDIKLKTRMFNKLKTHFYDLSKNPFGCRVMQKLIEYSQGKEDIQNSILSQLVQNMRSLIYDSNGNYVIFKMLESYDKSKMEFLIPIVEESFHYMAQQIYGCKIIHKIIQQYAPNYIANLVKQSIANYSVLSQTEFGNYILQHILQFWIVSPEKTRLIQLVIQQFYQLSINKYASNTVERALETLSKPELITVLNWLLFRNQAQQQFTYVASNFVQLANHQFANYVIKKFLILIDQQMQQSILNFLIKNQQECQALKSTLHGQRICSLLEKQVKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LEZ9</accession>
    <name>A0A8S1LEZ9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0370277</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LEZ9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8065449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="61" mass="6955" checksum="FCA1F5424BA01166" modified="2022-10-12" version="1">MKTFDVDQFNMSISRQLVGQSILKKGGLLVPGHAVEERKFWQNRNLKLFSTRALPSSCCRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LHD0</accession>
    <name>A0A8S1LHD0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0400170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LHD0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8067217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="21321" checksum="46C2866B20287A92" modified="2022-10-12" version="1">MFILEEQEIKLMKEQQKQNERQKDYFFQKCNNQKLFNRQRLDQQDSKRKLQYQIFFKNIKTKSLKNCQLNLQNSLINPLKQNIIKYCYKIYLFLQILWKLPYNMIIYHAFILMFQVYQLMKMIMLLILIKKLLLLKIQILQKEYEEIDREKREKQEKKKNERGKKTLGNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LIW6</accession>
    <name>A0A8S1LIW6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0380219</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000037">
        <property type="protein sequence ID" value="CAD8066002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ILITHMD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043366">
        <property type="entry name" value="HECTD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46435">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46435:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="4">
        <location>
            <begin position="3516"/>
            <end position="3858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2204"/>
            <end position="2227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3868"/>
            <end position="3896"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="3188"/>
            <end position="3264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2204"/>
            <end position="2214"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="1">
        <location>
            <position position="3834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8066002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3896" mass="455949" checksum="BCEE38FE9D4BA5C8" modified="2022-10-12" version="1">MSFPQHIPSTSLSFLDPSLLIGNLDWLYLLSMKETDGLSEKSLTKNEDVSGFDAFLEQVDFTRSAYIKLRQDYFNLIQHSSRNLTESLLNSKNEYFSRASMMLNKINKSKIEKPIVMAQYHECTYEVFKQCQGVTSCSALQSNPTRWPWGLEDFHKSLCKQELLSLFYEPEYSRQIIDKNLQRLVKEIDHKQLSKILIDYALRVGTFKQILSLCDLNIREIIKLPRLQQKSRHLHYMRSLCDQGGFTITQLQQFTKLNYHQMTMTSSQCYLYILVSSPVGGMYKVGTGEGGTIAGKIYLHQPIKKQDDVQWVFLQNKLYLKQQNGTLDIICPETFQTLSQMTLHCPELFQHPQMQAINKNYPLITDGEKLFIIGRKLNITKIEGAPAKVTKEPAASNPRQQKKKEAEHQPADNTIKIMDFILFEFDLNKPTGNVQNEEESDKKLLAELYESFQGYFSKAEISKALNLNKNDMQNAAQWLVEEGEKERNKTTVSVSKQTLLCQAEITTDISAKQVKSSADIGCKEGSILQPQIISNIIWTMDKKYLTAYFEFGIKIFSKDPADEKELDQTKDEFNLRDHYSTKKPATSNLHLKGTYITTVNKYYNEFYQNPNYIVSYEHSTKRFYVMHIQWNSTDLQFPALVTIGSDFIHRPLNLNLSQATTFDEEVVNFLLQQQEQRYDQEWRLNDWYVAYQLMLKNLRSISEHFSEEEVKKIQSEKSKYVHRLNKSIQSINNTRTEVEIKKVYAFCVEGTFEELKQIWSLFKKFHKIQYLQLLQYWVQYSEISILLNVGIYDLLKEIGEYLIQNWQSDLHRNIFIKGWPLFLCSNQLQLQYLRLGDNQLLQNIISTYSSIQPETESTILKLKQKNINLDGDFKSFIHKKGAQHFLIEPLSRLLVEVVQNVKLFQKVYQKGYFVDYDISQINQQFWILAKEWAIQCDLIKWQFLTMLFIMAFNENNEWEVQYKLAFNILQVFELGIANKDVKELIIKHNFLTHFLLQLFLSPVLQCPIDCSLLSVLMQINQNIELPTIPSRSQGIDPIGYKVFETSHPYERQKVQTFEDTYFPGALALAVEFDPRCSSDQAHDFLTINSWYSPQTGPFGLQTKLKDPMGVSYRISGKLSTKRPILMLGNVIQADFSPSGQVRNEHSLNRWGFKITIHPVYGDTYHVSNVLHMVILENIQSLLGFNENRTQYEENNQHLLKWNILKNGRAGFQYDKHLIKVLGQLSISNMSYDDATLLHENAGANSQIKDKYQQLKRDLIKVNYKSRDIQKSNINLLQLAQQQNKYISQILDKKGEFYKVISDIVSQFTDSIQYRTAKQKSAMKAHIEEFKQIEMLVILVTLYHSGLLDEINLLNLEHHIDMLQEARNDVINYMLKEVETMIEYQQNYEGMSLQATEMMEQQKLKQINEQIENTLWDMLQKRLEGNSDMIYKICTQQNLPIIENNPNLSLRQIYNNILAKIRESKLDLVNPYQHIVQVIKKRILILFNISNVQLVGQQQMIQIKSQQSVDSGSIKQLKPLGIERSISHQIDDKNIDSQKLLKFRQWLDSYQKWKVLQNVNLNQISAESLDIPPVKSVILFLQQNVDESLIQVMDLHNRRCIRRIASLKFLNEFQGQSGGILCQDVMNCKIEFASSNIKEILLQEVVKRLNYLMQSIKNDYATYLNQAITQQIDGTLLEQLKVLCMTLCEINQLFQVDNNILWKSREHDYNPHFNPIFKQFLNNIIITGLLSLAFVNLDQQYQSVAILSQNILNNVKETLYIIADNLMTDDILQQLIQILSQELGISLQGDEINCRLLTKLYDLIGVKRVNFLLTNILDCLIIHQNQDVPSSLVKLIFFILHHTNTPSTMRLAIRISKYFNNINLNKIKYNPKYLWIYYENCNLQQIKVLQENKELYFSEALIERVGHLVGQNEDTNYQNINIILQNELTNVQKKMINSQNTNCDQNNNQQYRVILHLCQEEEISILLKILYWWEELYPSLKLKLPQTYQEYLEMKEKEKKREEQKTQQQEVIAQQTEFQSQRQQQQQQYKLLERVLMTQPEIQQKDYLLGWGFNYSNLYSDMKTGPSDNKKHSSGRVKKQIKKYWRPLNFQELERLYEELYKQNEDIKQFPDEQAFLRQRNFITRIHSHIKEIKNYGSLLTVYGCIAFSEIFNLQKAQVLVELIQSALNGTLPNIPMNDLSEEFKNKADPNKYILLGAIKEIAPPKSKSQPQKAPTQIPPQPTSGKSQMTVSVCDVYTFQTFEFRMDYITLLPYGNTSVNFYNEINRSSSFTQQLVNGQIVGQLIYELSQLLCHQQIDINKQSNYYKLGYTTILSQQLIQIAPGIPVQIKKHKSYDKQYVVSGGDYCGQKELRLLSNNDDKLNLQVVKLDEIELIQQDIPQLITQEQLLELIEKENDKFILRNLFIIASKLEWKGQNVDKIINKILNLANLYQGDNKFIQAWERLIDSRLQPNYFYPIEIPKSIKFLQQPQQVEQEDYKIEPFVENVDNTIIVGSTYMQSLPEACIVSNELKLVQYWEKNILPKIYDYVKGSLRPYEIDDFFEQIRQQLRKGEQHKALEIAYIVCDQRMPNGVIIPESNHDWSTLTIEEVQAGQYVLCRLIDKTSEQLYSKEYLHYKKQGQTFICGQIIGVDQRSSSILITHMDINNSQLYATWFPVSSLKQVQLYIPPNANAYQQETILKEYNRELELELSKQARQLYILLSQTINVDPITYLQLAMQFEGSCINGWLTEQLIDENTKTNQKLNQFMQNPDLIFGWLSKSFDQIQQFVNNNKLLLDLVKSHKKPLELNSDLDIAGLAIVFKNDASLCTCSGINFYKDKLGINDFYTISSGKETRGRLSPIILNQKTVYCEYYFNGEALPIYQQKLCVSRLPCVVYKIPTLWNTVCYVIDQMSTIFIKNRDIPNLKNINHLLEQVYQQQPHILNQSIIKLLIRNIRKLQTISEETLKDELLSQLVNDAVKIYNIQKVEPNVLYSSYLQDITELLAVAIPNKTKIQIPDWLNAYRYLIQIAEFIKCGKNLYNEQIEQINGMLQSNQWDQLIYMKELPCDIQDVLQEQLVRILNPTIDIYRNQKQAIVLIDGFQQLNLENVEIQLPPEPVQETVKLWECEVCTFAENAWDSSHCSMCDTPAPANKTPYKGAEGQSQQQEQQQIQINVDEEDEKADRRENQFFEDLIEKIKEKIKIYYTDQQKDKEQKIKQINQAIEIQQNLLKQLNKQQEKKQKQLIYKSKLQQNKQPQQQLQQQKIQDCKLKLQQLTSELQLIQEIKLENNFQIYTGKQIFEKAKAFLQEFLFNRLEYFRKKINPKQLEDISSMDPIESWKYLETLGYDLHYQQINNPKIIQILPIQQMKHLLKLIEFDICKETKAVRTYNPRNIRNQIDEQAINYSDFINCLTKYQDLVNAPLLVVRQSWAIIKLFNRYLETCGQFINMDVPIGTSQQFHQNTIFLSMGNYMQVLRQLCMTPTKVDIVQRVLNKTSISRDSPPKINVERIRLANRNGKTSQQDYLFTMAYEQMKNIPPTLLRPIKPQGTDPFLAFEVNFKGEDVQGESGPYRQFFADISKELQSSQLLLYPSTNNQAKLGNHKDKFVLNPSAKSNYFLHLYEYLGVLMAIAIRTATHFSLDLPSVFWKQIVGEKITFEDIEQIDATMCNLINFMQECPESTFEENIFENWVTLRTDKSVQELKENGSKIPVKYEERLEYINKLIEVKCTESELQCENLIKGFIKIIPSPLLNWVSAEDLELWVCGRPIVDVDLLKRHTRYSGDLNENSDRIKFFWEALYELSEQEKLRFIKFCWGQERLPANDEEFDRNQIRFMIKPSTVNTKQPNKALPKADTCFFNLELPNYSSKDILKKQLLTAINFDCDSMNADPRVNLDPNARHRRNREDDEDDSLFEERDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LL52</accession>
    <name>A0A8S1LL52_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0400164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP97 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LL52"/>
    <dbReference type="OMA" id="KMMEIDQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038792">
        <property type="entry name" value="CFAP97D1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029488">
        <property type="entry name" value="Hmw/CFAP97"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33768">
        <property type="entry name" value="MIP11318P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33768:SF3">
        <property type="entry name" value="MIP11318P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13879">
        <property type="entry name" value="Hmw_CFAP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8067205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="194" mass="23201" checksum="3C2A483BE9DBB78E" modified="2022-10-12" version="1">MWNIISINPICQKKKLVSDQERLLLNCKNMRKTINNGEPWKPSHSLSKVNNKYSHQEENKIMHQNNILLSKMMDIQRHNLRSQSSGQLPLSLSHVSNLNQRKFEKFKINQENQKFLSRLQSASSIYNKNYWQPDNEKNEIYKQIILKRTKLNQDYNDLARKSLETRSTGRMLSSQQFKRRQMSDEQFQYQQSPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQP6</accession>
    <name>A0A8S1LQP6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trichohyalin-plectin-homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0420273</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQP6"/>
    <dbReference type="OMA" id="NARFQQN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039986">
        <property type="entry name" value="CFAP210"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043597">
        <property type="entry name" value="TPH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28663:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA- ASSOCIATED PROTEIN 210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28663">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13868">
        <property type="entry name" value="TPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichohyalin-plectin-homology" evidence="4">
        <location>
            <begin position="115"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="149"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="304"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8068665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="477" mass="58062" checksum="311CEA9422E97554" modified="2022-10-12" version="1">MLSNTSRGQQQNQFSKTQTQTLIPLSEINHIRGKLKGGNLATEERQLDKMRMKANSEERMKRWPTTIAAYKQKRDQHRFEKFAAAEEERRQIDIVEEIYQKEQKKITLEKANKQIYEDGDRVKAFHGKMLFSDVLQERDEQIVMEKYKKELLKQQEEIYQQILEEQLDEYDQKQIVKQKLQQQKKKEQKDMLHKQHEEMKEKHLTKLKEERIEGELIKQKVKDALEEDQKIQRLKQQKILENQRLVQLANDQLKDFKVQQKIKEKEEDEKIRLHAEKKQRIAEMRKVREDLKFHEKQEQRQKLIDRQIQQLEILKKAQEEHLNKQIIEAQVKAEEVEKIKKQKREQMFRAIDYSRKIKDEVKETESKSVDYHKKEFQTYWEKRGKELEGIERAETTAQRERRVQNARFQQNQIDEKQALREKDYIEQLDQQEEKQRKIEKEDEIFMMWASQKIQEHQKEGKNILPLIKELKNLSKVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSH6</accession>
    <name>A0A8S1LSH6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0420172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSH6"/>
    <dbReference type="OMA" id="WVPTNKE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8068463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="211" mass="24987" checksum="14E2498605DABE99" modified="2022-10-12" version="1">MFIKNFFKKQSRFERKLFESSIFDNQETVDLEDVKQMLQGFPCLSEIDQLVQSMTLVQVCREFLRLIPQTLDNHKKLKVLLTMHVLMGDVKHGRLFVQQLNYFNGWVPTNKEDILNKFIGIQTMIIHKLASIQDICTRSKLKTSIEIFFKDIDTSVIQFYKAINCLNFILAQYDLFLSISKLHNRTVVMEIYLLIWNDLIAMYLMLERYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LU93</accession>
    <name>A0A8S1LU93_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0430258</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8069221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LU93"/>
    <dbReference type="OMA" id="PPAFSYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20621">
        <property type="entry name" value="CYP5011A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000091">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF50">
        <property type="entry name" value="BIFUNCTIONAL ALBAFLAVENONE MONOOXYGENASE_TERPENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8069221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="58204" checksum="A65E2D67CDF0940C" modified="2022-10-12" version="1">MIYIILGIVALIYLITQPLLVVIYFKIKYGKRVIIQYAPMLGLLRTLSQSRAINGDAFESLSILIKNNRKADILVTNLLFRPLIIFLKPKYYRIFMQQYQDYSKFPMIVDKRLISKGMLMEGGAKWKQQRNLLGAHFEYNKLINLIPMINDVAQKKLTNLPNSIFPVMNIASSITGEVVLRAFFGEDIAQLSLNGQDPSQCVTQLITRFGQLITESKLFQIKRALLEEKVFSLFPRKIEKDLYELNTVMLDHMKKAIQSRMEKLKNCKDIVHKDYLDVYLDVYLNKNDQMNPPITEDEILQQSITLYFAGTETTANLVTTACYFLAENQHLQEEVYQEILGVLNGEQIITAKHLQNLQKVTGFLMECLRLKPVAPMLISKQAQKDLYIKDIFIKKGWYVNYMVLSNAQNKKYFSNPKKIDITRWKSDGTLKDSNIDPFAFVPFSAGGRNCIGQYLALIESKIVISKILMNYKLKLNEKIIRTEWIAKITYGLSNQDYITWESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LX60</accession>
    <name>A0A8S1LX60_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0510004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LX60"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="199" mass="23894" checksum="5D54DFBA3CEFBFEC" modified="2022-10-12" version="1">MLMKLRGQRIIFYLHEIYESQGNLYFIMEKLDHQLFDDYYHEEIQLIAQQLIMLLREFQKLNIVHHNLTQKHIMFDEKNIIKLISFGNTSSIKEQTDFFFDVYQTGEILLELYYINQDQLFSKSTYLPDHAVNFIIGLLNLTEYRLNINLEQTHPYFKSLNGESQLVKIRSLLPKRNLSDLKKNKKQQDTDQDQLTIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M032</accession>
    <name>A0A8S1M032_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0480124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M032"/>
    <dbReference type="OMA" id="QFEDKIH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="272" mass="32278" checksum="8F24BFE7941466FA" modified="2022-10-12" version="1">MNKRNKKPSLIAQLYFDLIQEMDNSQILNQDDTSLSYNIIQLFKSIDQMRQQLQQQLNEHIKNDYESVIQNLEASVRKHIRTQYQLNLQIELLNSKIEELIKEKEIQVKQFEEKIHKLQLQLQEKYTESVGNQQTNFLNHFSQLDLNTQKKTPSAYEKINKLMSNKSQQNILNTNGFLNQDERSLSQRKTSYPLRKSIDTKLLQQDNKKRQICFQKSRLQQSVIKQDYSCSQTQRDEQISVDQRKTKKIILQLKGSIPQYSNIHLTRSGLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M2G5</accession>
    <name>A0A8S1M2G5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0530277</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8075008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M2G5"/>
    <dbReference type="OMA" id="IDAYVAC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="6"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8075008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="154" mass="17428" checksum="8556E584D58DCD7D" modified="2022-10-12" version="1">MNLTEEDIDMCQRAFAEMDEDGVGAIRGQDLKIALEKIGFKPSENELYKIISEVEETNTGLIRFSDFLGVYWKFKYSNQDDDDQDTIDAFVAMGGNPDKTGSISTDKLVQIIKSEFELTIDIESLIRDVDKDNSGLIEFGEFKDLLKTSYANDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M2W4</accession>
    <name>A0A8S1M2W4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T0520153</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M2W4"/>
    <dbReference type="OMA" id="NTIDSHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="432"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="492"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="522"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="584"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="717"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8074247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="788" mass="92739" checksum="DB3676D96FA18C8B" modified="2022-10-12" version="1">MFRATEIHLYKLYVEREQAFHLLTKVGQMKNVNLVNCSSNTFHEHDYYKQLKRCDDIYNKIGEIKHLLHLYNKQIHYCPNYEVFIQNIKITDDQAIKIEQELTHKVQFILNQQANLQQIIEQSNRLNEEIAVLQNCKDFIYKFSGIQLGYIVGCMNTIDSHKFNRIVFRISKENGIVKFKNLNNQRCLVTLVFAIGKHENLKNKLLKICEAFSVSIYQLPEESKVDNKILELQNEIANLDIIISTTKQEIDQQLDYFSDIQVEKLLNLDEIYDYGYCSYICELKIILDIISATYYHLTFFDAKSQFLIGHIWCEQRDIEQIKQFGVQIEIMQDINENLYEPPSLMKTNQFTYIFQELVNTYGIPRYDEINPGLFTIITFPFLFGMMFGDIGHGIVLTLFGFYLLIFGQSIIKRIKLQNSSEYLAYADIQSIYQCRYLITLMGLFATYCGFIYNDFFSLSLEYKLEKFQLGFDGKWSMSESHLTVMNSFKMKTAIIVGVTQMVFGILLKGWNCLYQRKYIDFFFNFIPELAFMLSTFGYMSFLIILKWLTNYSNTQESPSIITTLLNMVFTLGGIKGTEMYPHQVFFQTILIKVAIYSPIIMLLKPEVIRMKRRLFNLRNQQIVYNELVEQEHVQVELVKEEKNQLFGKLVESRSIKEEKHFDFSEIYIESLIECIEFVLGAVSNTASYLRLWALSLAHSQLSEVFFKMSLEPQLQSGSIIGICLTFMIYAFATFGVLMCMDTLECFLHSLRLHWVEFQSKFYKGDGHIFQHFNYLQFLDQKFQFSTRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3K8</accession>
    <name>A0A8S1M3K8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0490124</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3K8"/>
    <dbReference type="OMA" id="VIEYAEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007288">
        <property type="term" value="P:sperm axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043195">
        <property type="entry name" value="TTC12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46540">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46540:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="209"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="288"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="55"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="113"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8072431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="846" mass="100313" checksum="F3E049B393BA9677" modified="2022-10-12" version="1">MQPPVFDNQLDPEEMEAWLAEVELVHEKIKKLSSEQANANDDDEEIIKIRQKRLEQEKKKEDAKKQLQIQKEEEIRKGRKGKGQNKDYASFCKYCQLEYVKQTDQCYWCKRETLTQEQRYNQLLEKVEKYKEDKNRKQQKKQKFELMEKTEKILWKKSTFTHKKWEYYTSSEEEIESEPIVPKDDPNFKALELDMEQRKKKKEEDMKKAEELKNKGNEYYSKGDYDHAAWKYSQALELVKDNKTLWLNRAITYIKSNKNKKAINDCTKVIEYAECFENGYTQSRDSCCKAFARRALAYYNRDRLLEALNDINQALELIPDDKWVQNLKKEIEAKIDHYQRLKQNEKEEKDEEEPKQDNQDQQENNNVKDKKNFMDDNLTYKQLIDKFVGEEEIELLQLFKVLKVDSNEATAYFYEKNGLKQLLKIISKNDEKLHDLQNILASLPALILQVYQDKSLLYQEQFLIQYNGIEVIYEKIKQLLKRVENKSQSAIYDTISDYLDVLNVMDEDKPRSIIQQHEIIRTKLYPELFQRIISLYKTEKDVVATFLSFCSNLCYGQSTPLKNVLFVNIQEIIAKFVEIFEKAEIKQANIRMMNQLCNLLSNLLTEEKYRFYFLSKELNPNFFKVYLKFIKAVQFKDNEYNSLKQNGIAVLVNLTFQINQVQSEYVSKINLLIPTLIEYLETQQDGLVLERVLTVLSKVNYEENIFIITKFIKQYSNQSIRCLLQWLSNKSPQVVDQLKQKQTLLYELLEQLKQQLQSDNEIIYCNCCNVIGYIIDLLSPINGEKNACSFFNQMIPRLLGFVKDRTGNFRKNSAILLAKLTKDQSNLQKLRDLHGIEILQSVMGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M457</accession>
    <name>A0A8S1M457_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0530163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000053">
        <property type="protein sequence ID" value="CAD8074807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M457"/>
    <dbReference type="OMA" id="YINVKTY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="134" mass="15496" checksum="C0EC226D2779D6EF" modified="2022-10-12" version="1">MRSQHRKATLNIPTILDLQNPEWDVILKSWAYINVKTYELTINKGSIVNKKILYLNQKEESINYKVCSSMPNIIEVKTETINLQKDQKDYIKLLIKAPLTPCKLHVKIALMDIKGDTVYEGMEFDLIISNITSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4W6</accession>
    <name>A0A8S1M4W6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0500121</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4W6"/>
    <dbReference type="OMA" id="RIPYDYK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08215">
        <property type="entry name" value="STKc_Nek"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="4"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8072933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="394" mass="47357" checksum="C9F06676FC621445" modified="2022-10-12" version="1">MEHYRKIELKGKGSYGQAILVQNKQDRKFYIMKIIDASKFDITEKENALNEIDVLKNLHHPCIIEYRESFVDKNKYLCIVMDYAEEGTLYQKLEQQKQRQEYLKESQIIEWFTQICLAVKYIHDRRIIHRDIKTQNIFISKGEIKLGDFGIAKSLINTEDLCQTAIGTPYYISPEVCQRIPYDYKSDIWSLGCMLYEMMALKHAFEAKTMEGLFLKIISGKYQPIHINYSQELIQLLKDILNTDPQKRLNINQILDYRIVRKSKNEFIQRKTQNIQKIQFISGQINKNENHFEKIRYYQSEQKFNGESLADQIEKMRMDLEEQLGVEKFILIYNSLIRKMRKFLQSFVIRLQQIKLISFLYQNNQYFDFSIYINYTFFQIIKLFIKFIVIFQFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M6H7</accession>
    <name>A0A8S1M6H7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein/NADH dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0510099</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M6H7"/>
    <dbReference type="OMA" id="IRECEGI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000592">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016464">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B8 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005822">
        <property type="entry name" value="NDUA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8073513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="100" mass="11645" checksum="61BFE3FFC235B442" modified="2022-10-12" version="1">MSWYKQLGNSVKELRFVFCQTCGRSEGIRNLVSKNYWQWKDANPHFPFVVRECESIDPYILIRYKYGVEKKALIGNLNESELEQVIGQLVAQSNKVNSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M806</accession>
    <name>A0A8S1M806_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0520035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YLLNCFE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="264"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8074013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1351" mass="161917" checksum="0FDC1232AC3FF273" modified="2022-10-12" version="1">MKQSQIFTKSFYQTHDKNTRKFLTGQQIEMEKQPKQNGKILSRLVRLEETHMVQIKNSKQINYPIGFLLITKQEFEKLPENQKTFFSWTTNKNKNVRILFDRSSNGLNKTQYLQFVKPTAVYFENIETGMRFAEFLKFKNQQKLNHFNDILIRLYQFWFHESSQKIIQSSIEKMHIEAQQIQYYQLKEQNEFLLDYDELLDYDKTIYEQMFKILYNMAEYEEKNKKQTIKQQKVEILQQQENFIKNKEFQAIRGLLKIWKSIALDTLREKEDLEEYQQKELEDEERKLQTEIEAIENEQQRISQIQQSQIQQQYQKQNLPQHKDLHLTKFKLIIDQHIKLFNPILYIQFKSLESNNLGKMEFNKGQEINFMQLDMKGWTTDLEVILTGFQTQDALDIVIYNDYQEVNEKMLARAQEEQNNILAFLSIAKKVMQCRIYLRDFEKNLNNNRRHFASLEVSANLQQKVTGNQLQNVPMIQIDIHEDSPFQISNSTVNPFYLDIIEVPFTVQEMKYFARQLKSPCNSLQQQWDSFKIDSLEFALQIRGQIWVNSKQSEDDLIEIQEFILEEKGIEEQKSKFIELAEYLQLNQIQNKQCFVKNKIIEACKKGFTKQSRNELIPIFLQFNIKIAKFTQDIGLEWNQNESLLNLIQNEVNQYLDQDTIQFNNEKVQNYIDSLQFKQITVWTDQYIKKLQQWKDRIAYLLNCFELSNHMKNKMILTIRLFQLFNQREDCDDYVLKIILSLDYKFMIPKINCEKLDFEWFRIHLNNLFSDFFYFDIWLRVFDYIIGIGYVNGDYDRVIASVIGGILNEVDQNKYRTKEEFMTGLNIYGKLLCDPEKLIMSCYNSYIQQEFIYLESDEQIIEILTQNGNPLEQICNDQIEQQQNNQQSFIIDVPEEHEGSIYEIQEQQSENILRSQQKYLFKQKIESIHILIHSMYIYQINFNEQNIVTIQNQKRSEQDGLYFELPFFSLILEIQINDNFIGQIDIGYLQINTIYKNTLILKDNYDMQRHYQISELEYSILLIGEGLQYERMISKDTAINNFNDLAQQFKVQHNFLAHPDTFKIGNILNLIQFKQLMQQYFKLDQASYNLDELYQKFLLKGNQKIYLIDILLKLTQDKNKQLEILNLFIPNEKISHKQLRRQQLENSDVKFLKVLISDQNYEQNIDLTNYFNGLLINHYQKYNSYDMLLIDHNNRLYLLDNAGSFLSQLRTQTLILRLKFSSFGNIRNIQYQLTKQFKLTQELKISANSIKELLDNNKRLLQLDNNLNLINEKHSIFNQHLVYLRCRDGSKQLYTCDVIGKILNKNNELQYFVIQMENNKRIIESKENVLLFNNISIDELDVYDKELKQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAG4</accession>
    <name>A0A8S1MAG4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GPN-loop GTPase</fullName>
            <ecNumber evidence="9">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0570022</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8 9">Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Binds to RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="9"/>
    <dbReference type="EMBL" id="CAJJDM010000058">
        <property type="protein sequence ID" value="CAD8076719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAG4"/>
    <dbReference type="OMA" id="HEVCLEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17870">
        <property type="entry name" value="GPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000888">
        <property type="entry name" value="GPN-loop GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030230">
        <property type="entry name" value="Gpn1/Npa3/XAB1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8076719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="287" mass="32903" checksum="C0E7F0049DBAAD3B" modified="2022-10-12" version="1">MDIEFQIKENKVAMLIIGMAGTGKTTFVQQLSKQLKNEKHTVINLDPAVYSLPYEPEEDIRKSINYKELMTKNKLGPNGAIMTALNLYSLQLNQLIEKIEKSDTTIQIIDTPGQIEVFTWSASGNLISQTLSMSMPTIIFYVIDIARCQNPNSFMSNLLFSCSIFYKFKLPMVIVFNKCDVADSKKPLEWLRDYDSFTEALKNKDTYLSTLSKQMVLTLEEFYNNFTVLEVSSLTGQGFENINDVIATAKQEYMNITLVDIQKKMNDQKQKNYDKQINQISQKIQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MDN3</accession>
    <name>A0A8S1MDN3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0550093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MDN3"/>
    <dbReference type="OMA" id="QEYQPIR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8075823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="176" mass="20793" checksum="F41568D7FC63246E" modified="2022-10-12" version="1">MIESFKQQCEQIYSENQEIIKQLNKLKQSTLENNQEILKLQNLVKGVEQEYQPIRDKIQSIKDQFQDSELNKQVIYDSFDELENIIIRQIAEQFKLNKKSKKTQKKESPQIGKINNENDQNTINLRKRTIKQQNIDENSEISESSEPIKAQKKKVKTNFEALDKANCLINEIFNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MGK2</accession>
    <name>A0A8S1MGK2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ABC transporter family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T0600171</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000061">
        <property type="protein sequence ID" value="CAD8078629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="FTITMTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000610">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000140">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000151">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002273">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="399"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="910"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1034"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1132"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="263"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="607"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="936"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1225"/>
            <end position="1458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8078629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1533" mass="176587" checksum="62B9404B22A9A889" modified="2022-10-12" version="1">MKNKDNLSQFAKKSVFARIDQKIDNSIIEDQHQDNDSKTVIQTMQKEMTNFQDEKEKEDLSDDDSPSNNIDFNQIYRNEMMNLEQNVPISQPSEQIDENYQENQCIMSKDTTYQKQENQAILKQLPHNTQLTSVEQTQRTEQGLLEIFKNKSNKIVPEPQNIQSSLKPQEFYPNTIYENYSFLSQIFLIHIFNFMKTAQKLIKQQGIKLSLIHLPKQDEDDTIQPNFQKAKKLFNLKISEQLTSFSLFRTFFFHDNKWLTLKIVLLAMSETYSRFIMSILTQTLIAAVKNGDKQDAFIQAMILALLSLIALISKHYQLYIINNFSTKMRMILTNFVFDRILKLKAYQINELNMGKIMNLVSGDINAIEFQLNFVYSLAIIPGSIIFASVILWLRFDGPIGLIAILICIIIYPLQVFIQKQVQRVLTRAKQKQDNRITSQNQLIEGIRLIKMYVWEKAFSDKIQQLRKEEVMCYFKIHFLNLIDRSFNYSVHIWGSFLFLLIIYFNDIYIDVSSIIGTIQLMSMIKYYCVFQVSYAFQAFMTLNVIFQRVVQIMTSPSSMSEKKRHILTSIENGSVIRKSNTIHVNGKESERNQVKMQSSVIKRTPYVTMSEVGLRWKARSFPVLSDLNMEVFPGQLIGLIGRVGSGKTTLLSGILEELPQIDGEFSVKKNISIAFVEQDPFIYTGTVRDNILFGKVFELELYRKVMQVSCLDQDVLSFQDGDKTQIGEKGANLSGGQRARLSLARALYSLADLYLFDDPLSAVDSKVARKIFTYAIKEFIFKFQPQYQQEKHNKYHQVFQMSIPAVILSTHQIQFALECDYVIILDKGTIITQGTYKQVKESLALFNSSIVEPIKKQLVRKQTRLATLLNKQQEKVEKKETVQLFSKEELNQTDASWQTYKRYFLFWKPVFMIIIIIGQNVATEIINNYYYRAMALYDETNRSANTQLFYNAGLLVLAAYCNNIIKYFLNIVGVLTSNNQIHNQMLNRLIRAPISYFDTNPSGRLINRFSTDLSLADNQIQQIITDIFEQGSQFLVSLVTIAILQPFFTFPLIFTITMTIIIFTITRQVVSQLKVCDLIQRSPLFDQFKVCLYGITQIRINNNKEWIRDQFSILCNQSMQANLIFSYSQRCFGFYIDIFGQFTNIIGIFLIIAMIEDPTLFSQALLLLSTFNTQAGTLRQFMAFDSIMNSVNRMFEICDIVVEAQVTQPIDEDLIVIGWPEFGKIQFQNVEMQYRSNMPMILKGMSFIIEGGEKVGVVGRTGAGKSSIIQTLLRITEISPNGNILIDGQDIREVGLAKLRQEIAIIPQVPFLFKATIRQNLDPLNLFDDSKVWKVLKDTGLVEYVLELPKQLQSEVQPELFSIGQKQLICLSRVLLNKKRILILDEATANVDMATDAFIQNTIKEKFNDCTIITIAHRLNTIADYDKILVLEEGKVLECGHPFELLATDTKTAIGIDKESVFAMMAIQTGVKNGQAIFDLAKKAYLKKILKNQYKRAETMLQSSVHLKQENFTQSIYEQESLLQPIDPKNQVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJF6</accession>
    <name>A0A8S1MJF6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0610093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJF6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="312"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="358"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="396" mass="44593" checksum="ADBADA95BDBC0C61" modified="2022-10-12" version="1">MAEYQPQAIIQQPALYGAPQTVTGQPQFVRPIVLGNSTYQQQQVTNQNLGQSGFYQGGGMLVNQPVLGASAISSNLISTGQAIKGESKIEYIPYEKTIMEYEEVRRQIQVPITRQITEYQAIQYETEYIPQVIQEKVIEYMPVEKVAERVEYQTVTRQNMLQNTVQQVQQTQIQPIVTTQTYQTTTPVVQTVQTPIVTQVAQPVVQQQIVQNVPQTYYSTYQQPITTQYIQQPLAVQQTIPAPVYQSSSPRLRQPTQVIQASAPVTTTTAYAQPPVLYQASQTRVGAPIVQTQPVAYGYQPQGQQLLTYQGQVPQPNLPNQSNQPISNKQPNQASTQTPKVSQPPQQPQANQVPSTMQQQQQQQQQQQQQKPQQQQPITNQQQQKQDKGFLAKLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MJV0</accession>
    <name>A0A8S1MJV0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0610186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MJV0"/>
    <dbReference type="OMA" id="ESRIEYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="231" mass="26397" checksum="340253A1EA7A5D42" modified="2022-10-12" version="1">MSYYPGYNPYGAPLGQSIAYPNTYAQPISYAPVQYTAPIAQSVVHPVAVQPVVQQSYIAQPVAVQQVVAQPVQQTIRGESRVEYKEYQRPVVEMETETVEVQVPKTKYVTDYYPVEYQTEYIPRTVYEQQTEYIPITKTVPRVEYETVEREVQRQQQVVVQQPVVQQYVPQVTQYVQQPVVQTVVQNPVIQSVIQQPTAYSAVRPAQTYVPTAYTAPIAQYPANLRNSRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MP94</accession>
    <name>A0A8S1MP94_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0650076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="177" mass="20433" checksum="8A95012333447850" modified="2022-10-12" version="1">MGPCQSQSKLKSSKNLQQPTLIRKTDQVLTNNNPSLILPSFSPNPEVISEPYDDTKKSLSQLKVYKFSYQRFYSEITQGNMKLSSNQKLVQHNLTGKIRVVEQYERNQQNEEFITQLKCYPLDNPRIVNVHELYIDEESXGIGLKLLKLSNESIQEIYMGSNTIFCFSQNGHLIFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSL2</accession>
    <name>A0A8S1MSL2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0680107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSL2"/>
    <dbReference type="OMA" id="HTNNRTQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="266" mass="31627" checksum="52D76926052B6292" modified="2022-10-12" version="1">MKSLQDIHKKNQQQRATFTLMNQMNNRTSMGWFTPQPSNDLQSPFGNTLSQFSQQGSEHNIIHNTRGNIHSWNQTLERQIQSREIARQVRKQNAQQSARQEKHFFGTINGIPKINSPNTQANQTTSMFQPNLIKVDPKIKRQLSEMSKRNFLHFLLVQKVGQKDEKQFYEYLKKTYNFEPPQPIEEQQIKVSKKGIKYLFTKEDDDETIKKTSQQILRMNKLHHGREVPLDKYVQDTYEQQLRRFPKVREKIEQSKKNNIEQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXE1</accession>
    <name>A0A8S1MXE1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXE1"/>
    <dbReference type="OMA" id="QIPGAGH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF60">
        <property type="entry name" value="SPERM-TAIL PG-RICH REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="360" mass="40648" checksum="BDDF5C877D0DBB7E" modified="2022-10-12" version="1">MGPGQYNPQTERKGGTLNFGSYSQRKMVLAQDTLTPGPLHYTINTHRSQGVSFANSKKTLQKPTRSPGPGQYLPIEEPHSRLGGSISRTSRNNNFDNQIPGAGHYPIKSTLNSLGFKFKQLHKRAKTDHMSPGPGSYSILQDSMNHGVKMHNSKRTWSYILKTETPGPGTYQSESFIQNRVSSKHSKTSSKEQSKILNKCFSQHESKQQSNKLGTFSQSKGNLDSHGSLTSRQKFKSNHITQQQNISDAYNRMREYGKLKVTFTKQVRKEEFLDEFSSSNPKKISRSPGPAIYADIKFKPNTKLGFVPTSKKGEVFKPKEGPGVGEYSKIIPFGKVYNKHITPKRQFFKFKDLNQFDFDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXP7</accession>
    <name>A0A8S1MXP7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0620173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXP7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="89" mass="10737" checksum="F676D67A14376A0E" modified="2022-10-12" version="1">MNNDLCLLIRQVRMPMRIQIFKQKKQPVFHKKTYTIPDKIYTDKEIMELYYKTFIKVEIDDTLALEIIQKDDFINCNINIRLGTLNINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N0W0</accession>
    <name>A0A8S1N0W0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0690076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N0W0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="559" mass="65903" checksum="D326A7A26C26F598" modified="2022-10-12" version="1">MKNENKEIIVHNFTCFKNLRLKNQLLILISFLIILIVTILVCSNYIHTTFVQFYFTQSSVEIYTKFSSAFIQTQLSSYQRYFTSLLDTNGQTLISLFQLYDYLRKKNKSQISPLNPQFNMNYGGKHVDIPKTIFQSTKISNYNSTLSFICYTNKTKFHNPYTEEEILNIKTQEQLQAFGPILYKGNTTFQSLLSAYVIRDNILMTYPCLQRYDIDNYIPENRSWYKELYQNYKSHSPYNKYNFSLTYPYLLFKSVNIGLSLTMPFVDEKLELIGGASFDLIPARLFANLIQQFGQDFTSIYLVSLDGILIMHPYNITSDQLPLYFYNTTITGFTVEDWESLNSVNISSKCKNSTTQKELNCLFNSFYNQDMFVSMQTIKSYNISIVVLISSQEFNKFQEEYNDNLLSQLIRKFYLTLIELIGVFLFLCILIYFTTNYLFRPIETIIDYAISKIQKRNYQPKMISSLLQQFLSVQIVQLYQSCYQFHKLLESLSFTKSKQCEEIENLQYPILKDIYSLGDYINFRKIKCYSKKKCEQSLEHFLMIKNIIRSTLKITINNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N250</accession>
    <name>A0A8S1N250_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0690165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000072">
        <property type="protein sequence ID" value="CAD8083205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N250"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035733647" evidence="1">
        <location>
            <begin position="17"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="385" mass="43761" checksum="C7C9E76407011E29" modified="2022-10-12" version="1">MKRLYSVAMLLLLVSATTVTNNQGVMDSYVNPGLQSSIDELTQSKIGHMVIDLAEVTMRVTGAVDSLVEGVKAFGRQIEERLNLENTQWEVVESEHNAKVISLSSQANQAEFDINREKKVLNSELIPRLHELDDILLGLKAKQTDNAQSFKEAEAARNSQHDSFVLTTTEFTDALRNIDEALDLLTDLFKSGEVRAAFIEVTDEQKHKVNKALRQVKANTENFGHQYSSFIQALTNLTEGFNFKDRQVLKEMCDFLNSLRLNIFDALTKAYKDEDSQKQQDELRRQQLTSEKAVFDQQYADFYQEREDKTVRISDVQTMISTREADLKAYQDRLRTENNNYSANLKIHDDIVSSVQQELAVLAKALQVVQTPPFLDFLNGRIAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N6D5</accession>
    <name>A0A8S1N6D5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0730189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8085143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N6D5"/>
    <dbReference type="OMA" id="NEVTHIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8085143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="760" mass="89926" checksum="263056BBC5683311" modified="2022-10-12" version="1">MKDSGLIFNNTKGTEFVIEDGIQSVRRSFQQQLQKTSLLIQHQKGQIKYIKDGSILNNEKQQLIEDELSFNRNLDQIKSLTWLGETQNNNMKVGRWNALWRGESLGIGGDYDKNGTKTGYWVEAFNHFWELSQVTQVGFYNNGIKQGRWNIKYKEQKIGGGNYTDQGLKEGEWIDLHQYFHYNNQVIFEGRYKNGIKIGFWEIMFREKMIEEFSFMLINPLYFSGFQNYNLNGIKIGKFVELIEDQLNFSQIVTVGQYINGEKEGLWDIKEKDKRKGGGFYKKNQKDGRWIDLEQILEQFKFLIKLLNNMERGVQKWAQMQQMEYGGGIYDENGFKNGFWVELNKSFSEDFQVFYTGQYVNGQKQGNWDIKQKHFREDTLQIIGGGNYNINGKQHGFWLDQDENYCEQLKISYSGEYSNGMKIGKWNINLEEQTIGGGYYNEQGMKQGIWIDLDENYSDGSYQNGVKVFQWQTNYQNQIIGGGYYNEKGEKIKKWIDLQFNFSNYCFIRYQGEYLNGIKQQQWNILYKENYTQTFIKFVRGCGFYNNNGTKIGMWINLYDNFSKDSLVTEIGIYENGQKSKKWNTFYRDSVIAGGYYNEDHNKDGNWIDLDENFSNYSLIKYVGRYQNGQKILIWQTYCKQKLMQELKQLNNFFRGGGFYNENGQKNGQWIDLHENYYKYCKIKYEGMYQKGKKQGKWNATYKNQIIGGGTYLYGKKYGLWIDLDENYYDDDQKIRQGEYLNGLPIGKFVCQKFKRKFNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAU8</accession>
    <name>A0A8S1NAU8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0780065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NAU8"/>
    <dbReference type="OMA" id="YEERQVQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8087171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="456" mass="52799" checksum="5B5ABAC85213E224" modified="2022-10-12" version="1">MSEIEQLTQQKQEIEKNQAILNQWVQLKKNLSSKFKNAAEAFKSLCKEEQDVLAIEDFGDYAKGLDLTQVFKDITLNDENFSKVWEQWEYKQKQNDHKLQIINEKLQLLTMLDNGEMPKEGLRGLDAAPNKRVQAIANNCETLEKLQEKLDNLVNEGQKQKEKQDIVQNEQDLFAQLLAKKSEIKQSELQVNKLQQKNNEEEEGTNQSKVKRSYIDSTPAYSRNKASFGSSGNLLQRSLQNKGQLGNSQISLKSSFIIESQIQISPQKATQNLKNYVPNPQQQLKSPYEERQIIEERKSQKANRMKGDLQSYISDLFQNEREQRQQIRTFDKPNSQGLAGFVTSKRLYQNASNKQSFVDTDSQNVSNRVQSIRYKLDDFQSQKLAQYSYVPRTVPDWTPDRQVRLSNPKPKQNSGKQTSPKYTPGNTHRLNLNYLKQEKEKDSHEQKQEEQPSANC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NB90</accession>
    <name>A0A8S1NB90_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0780125</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NB90"/>
    <dbReference type="OMA" id="GHVVETN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14832">
        <property type="entry name" value="AP4_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000010">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="7">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8087291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="145" mass="17331" checksum="6719AB1F011F4268" modified="2022-10-12" version="1">MINFILMVNKQGQTRLAQYFQFLSVKERMTLEGELIRKCLSRNETQCSFLEHRGYKIIYRRYASLYFIIGMDLEEENEMAYLEFIHNLVETLDKYFENVCELDIMFNIEKAHYILEEMVMNGQIVETNKTQILAPIHVLDKAKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCW7</accession>
    <name>A0A8S1NCW7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0860055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCW7"/>
    <dbReference type="OMA" id="EARIDIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="398" mass="48083" checksum="FC0238386A123B51" modified="2022-10-12" version="1">MSYSQKNFLNQPFYSPAKFENDQKDLMISQLKAENFELKQNDRDYQELATHLKSLEHRYNMLHEEKMRNEVEFRNRSDQTLKTIANLRNEIDNLKSQLTDKHIENQEMKAENLAFKEITEHRTQENQRVKSDIALLQENNRKLYEEKLRLETELQAAKDERKRLLHDREILNNTYEDVLDKLNEKEKQFSQLQTEYDNLEKQALIYKADVENINNEVKSKNENLKYTRMQYQEAQNYISQLQNDLEELHKQKEKFKQESLLYQKNYQQEADTCMELNAQVIQLDQTVKHQEKTIIDQRNEIERLKSMHMECLETTEELNHELELARRINDQLEHQHRDVMEELDKLSLTEEQAAMTRATKYKELKTKLIMGTKQLQQFSSPFKRFSTNKKSQLKQYEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ND67</accession>
    <name>A0A8S1ND67_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0830064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000086">
        <property type="protein sequence ID" value="CAD8089199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ND67"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035723980" evidence="1">
        <location>
            <begin position="17"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8089199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="392" mass="45387" checksum="3D0FD1476374744C" modified="2022-10-12" version="1">MKRFLSMAMILYVVTSTKIQNNQGVMDQYVNPGVQQQIDELTQSKIGHLVIDLAEVTMRVTGAVDSLTEGVKAFGRQIEERLNLENTQWEVIEGEHNAKVISLSSQANQAEFDINREKKVLNSELIPRIQELDDILLGLKAKQTDNNQSFKEAEAIRNIQHDSFVQTTTEFTDALRNIDEALDLLTDLFKSGDIRAAFIEISDEQKHKVNKALKQVKSNTEKFGHQYSSFIQALTNLTEGFNFKDRQVLKEMCDFLNSLRLNIFDALTKAYKDEDSQKQQNQLRIQQLTSEKIVFDQQYADFYQEREDKTVRISDVQTMISTRQADLKAYQDRLRTENNNYSANLKIHDDILVILSNLYSHLRNFYYSFSYDVLSRFSSRLIRKITFTTLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NE62</accession>
    <name>A0A8S1NE62_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0880042</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000091">
        <property type="protein sequence ID" value="CAD8091387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NE62"/>
    <dbReference type="OMA" id="STNFFDM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05353">
        <property type="entry name" value="hydroxyacyl-CoA-like_DH_SDR_c-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000185">
        <property type="entry name" value="peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051687">
        <property type="entry name" value="Peroxisomal_Beta-Oxidation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024:SF2">
        <property type="entry name" value="SCP2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="8">
        <location>
            <begin position="9"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8091387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="449" mass="49566" checksum="FEC2194E219C90A3" modified="2022-10-12" version="1">MSALRFDGKVVVITGAGNGLGKEYALFYGKKGAKVVVNDLGGSMKGTGASSSAADKVVEEIKAAGGIAVANYDSVEFGERIIETAIKTFGKIDILINNAGILRDVSFEKMKDEDWDLIYKVHLKGTYACTKAAWPYMREQKYGRIINTSSASGVYGVFGQTNYSSAKLATHGLTLALSREGLKRNIFVNSICPVAASRLTETVMSKEVLSSLKPDYIVPLVAWLSHEDCKETGSIFELGAGYISKLRWQRNQGYFFDTPFTPEDVKEKWDEVSGFGTNVYHPQTSSEIFEIFFNKEEFMKQQKEGKAKSTSPSQNTQPTNVTLKAEKIFNLMRAFLDRGEGKDLIPKIQGVFNFEIILQKGGPVIKSWVIDLKNGQGSIKEGKEQGDATFNMIDDDFERVCQGKLQANEAFLKGLMKIKGNMKKATLFTPSLFPAPTPENLAKYSQAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NEL7</accession>
    <name>A0A8S1NEL7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0870129</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NEL7"/>
    <dbReference type="OMA" id="CVFEYCA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="12"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8091098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="486" mass="57727" checksum="8A5E92EE2A69D708" modified="2022-10-12" version="1">MDLQVLKQVRQYKFRKILGTGSFATVYLGENLKKEIFAIKEIKLDIAEGSDEIQKLHTYFQQEIDIYLKNKHDNLVCLIEEFTEKQFRYCVFEYCANGDLNNFIKKNILNEQEVKTIFTQILEGMKYLHEKGIVHRDLKLDNILIDKDNIIKIADFGFAKYYQQNDIFVSYCGTPATMAPEILNQEPYDFKCDIWSLGVILYFMIFKKYHWKGNVRSIVDLQRQYKNFTVQFDNQVQLSNDGKDIISSMLVPDKEKRINYAELFSHPWLEGKLNQSQNLLESQCIIKQKLTIKSPGQIIGNIIKQQRRIKGEFCSILDQCIKDCKNGELKENFIIFRKQINQEKFQITIQSGDLVDKPEFKVFDYKYELFEFLSKLYNQYGIDYRETAQKEYELINFLRQNPLSNSITFTFDGLKTQDEIKIVRESIIEEEINNFEYEDWKMDTLEINNIKEGLVECMSNDRFRFYKLDQLYQSNQSKLNNILNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NIZ9</accession>
    <name>A0A8S1NIZ9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0890194</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000092">
        <property type="protein sequence ID" value="CAD8092052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NIZ9"/>
    <dbReference type="OMA" id="LKRCVKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8092052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="193" mass="22625" checksum="99B6BB73C400EE6A" modified="2022-10-12" version="1">MQDAKASEEFVQNEQEFKYISEQVKQKLRKGEYSTDEFYKKNVDELKRCVKMMETEAQMTSTHSKKILQNKILQYKKQLDVIEESINELLIKQKKTDNLKGNLFENDLIIEEIDRLTQETEQIALNVDSKMNAGTLALQQSKFKKQDLKSNLRKSDFTIQMMNNKITLDKASLMVIIILLGIIDIFAIYKKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NL56</accession>
    <name>A0A8S1NL56_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0940167</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0940169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8094003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8094007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NL56"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8094007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="126" mass="14103" checksum="884DD55DF720201F" modified="2022-10-12" version="1">MGLSSRIGRLAIFIFFVGFAILQLQNPQVAIKQTHEKTNQFLQLISQLTKSPLIQFPLDSYTLVVFSCALLKFFVGIGVLLKWNYVKLIGSIYLLSAIFVVHNPMLTQSYKDSIMFVGALGALQFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NNK7</accession>
    <name>A0A8S1NNK7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0970072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NNK7"/>
    <dbReference type="OMA" id="APHKMEE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="166" mass="19836" checksum="56F128D938828827" modified="2022-10-12" version="1">MQYFQILKPEIGISITRGILKGVVLIIIGKTILSYHPFDFQSDYPEYFARIPYKHPINPPIYAPHKMEEPEDREKYEKLFDDIIQISNNNREYYMFKHLYHPSLEDGFIIKEALWESDGERVLDEIRGEVRRQLRQAQNLELQNQFHITQRKVLFSKLNGQIRPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPD5</accession>
    <name>A0A8S1NPD5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0900003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPD5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="115"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8092131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="219" mass="25753" checksum="37E3F9B63A68873E" modified="2022-10-12" version="1">MKEFLKFLVHLAKIDNRFKISGSNALNILVVMQVALKNQNFENIRISNTSLFGECEYQQWSQFTWSLIFWIQLMVILIMSSQSVSLLMETHQLLVVMISLSVYGMSKQDNKKPKLDGHQYQVNSVCLSTDGNTLASGSSDKSIRLWEIKNKHYSDYRFKEIQVKNAKYPFFNTLLQKNNIHKIQIHLILWFTQTSLFQGQREFFIKNRFHLGELLQYNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQ02</accession>
    <name>A0A8S1NQ02_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0870182</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQ02"/>
    <dbReference type="OMA" id="EEIIECW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8091205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="45463" checksum="B4DCEA1D71145D26" modified="2022-10-12" version="1">MQFELIEIQSLVNKGRQQEALKQCDIAISENSNQVQLYAIKTKILESLGLQNEVIDIWEQGIQNNKNDFLFYYEKVFALRRQQKYEEIIKCYDQGIMNNKNQAIYYNFKYIELEKQDRLVEAIQVWDLAIENNKTDSYYYRQKIQALIKCFGEDNCQKQIFECWDYGIQNGNNNLFFIKSKVKALENLERLDEAIVFLSKEIQQYKNVEFLYELKTNYLKKLNRLEEIIECWNEGIHYNPYNKCFYQQRIKALEEQGNIEQIIEEANKCIYQFNDLIFYQAKAKCLLIQGKLQEVIDCWDQGIKNNQSNSSFYIEKNYALQLQERWIDSIQCCDSALLNESNNEEIISKKVISLIELHNFRQANQYHRLIHPNVPIPFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQ39</accession>
    <name>A0A8S1NQ39_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0950126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQ39"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="144" mass="16306" checksum="C6DE6F3899F9F039" modified="2022-10-12" version="1">MESIQMELNYSNCKWKNLKIHELNKLDDHSGWILSVCLSSDGNILASGSGDKSIRLWDVKNGKEILPAYKTYKDILAQFQTPLFQNNPLLLSNSITVLRISQIPLFQAQGALLLKGGFVNYGGYNLRQLFKSKGSCFLEDFKQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NSB1</accession>
    <name>A0A8S1NSB1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0980182</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="16"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="60"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="94"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="161"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="280"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="314"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="348"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="844"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="878"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="912"/>
            <end position="945"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8095458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1331" mass="156194" checksum="76FC6BFD469C4D5A" modified="2022-10-12" version="1">MKKEKQKKLITEELTWEQHVICGNQSYESGRTDEALLEYDKALILNPNNSLIYHNRGSATLRYHQLGILYYNMEKNQEALLDYNKAIALNPQDSKIYNNRGNLYSDLARCEEALDDFNQAIQLDPNFSDAYNSRAMLYVDLGRNHEALKDFDKAIELKAQSNVYKNRGILLFNLNRFDEALKDFNQAINYTKKDSTLYLNSGLNLFRSYQQLSYNPQIDMKKLQSIITYQSNQIVMIKWLIKVEQYFTHIQVKSNQPFLTFLKQQYQNQMILMHITIEAIIFNNYLANSYWKQNQINLALKDFNKSIRLNPKYQIAYDCRGNLYLEMGQTSKAESDFNKSIELNSRNSNSYNNRGNYLVNLGNYHEALKNYEEAIQLEPNNSLYLINLGELHLKMNDIQQASLCLLQADTHLKINKNSSLTNIHQFINKMLPILLEIITSFEKEKLLIKTLPLDSQVKLQQINEIKRIEASVVSKLITILQPISLQSNQNYLFNQIDQLQLIIQDIQKNFSLIYQTLQMEQEVDINDVNIATIQTEITNLINEKKNNKYLYFKSLCCNLISFMQFCLDIQQADKIIKVSSIQKEKQKLFPNQFCTFKYENYVIQHEIMGSLFEIIFKALGYQDNFKQIIIDRNFAQLYFIQNTFSKKLEIEFHLLAIELAQKQQDIPEINLFTDCVETVSNIQMKEIPIASNIFWKKGILDSIKIIQFIDNNINRILSDGLQYLRMIKQELFSEETIVIQESHHHSLMAVPTLRNEQTTYSLKDFNQAINYTKKDSTLYLNSDMKKLQSIITYQSNQIVMIKWLIKVEQYFTHIQVKSNQPFLTFLKQQYQNQMILMHITIEAIIFNNYLANSYWKQNQINLALKDFNKSIRLNPKYQIAYDCRGNLYLEMGQTSKAESDFNKSIELNSRNSNSYNNRGNYLVNLGNYHEALKNYEEAIQLEPNNSLYLINLGELHLKMNDIQQASLCLLQADTHLKINKNSSLTNIHQFINKMLPILLEIITSFEKEKLLIKTLPLDSQVKLQQINEIKRIEASVVSKLITILQPISLQSNQNYLFNQIDQLQLIIQDIQKNFSLIYQTLQMEQEVDINDVNIATIQTEITNLINEKKNNKYLYFKSLCCNLISFMQFCLDIQQADKIIKVSSIQKEKQKLFPNQFCTFKYENYVIQHEIMGSLFEIIFKALGYQDNFKQIIIDRNFAQLYFIQNTFSKKLEIEFHLLAIELAQKQQDIPEINLFTDCVETVSNIQMKEIPIASNIFWKKGILDSIKIIQFIDNNINRILSDGLQYLRMIKQELFSEETIVIQESHHHSLMAVPTLRNEQTTYCSFCSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWL4</accession>
    <name>A0A8S1NWL4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0950122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWL4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="199"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="241"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="283"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8094336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="413" mass="47198" checksum="84C52F196FF5B7B4" modified="2022-10-12" version="1">MHCKQFILQLENLLQVDKDQKLNVVTNNIKIIMNILKQIRDHDFNQNNYSTEIYLQTRQSLKENIKEDQKIIKSLQFLLQLTSLDNQFIQSGSNSLNLLIEMKIDLTKQSFENIKIKNTSLIGANFLRCNLSGSYFENVCVSRMKLEWGIIIQLQMEKYKINQLHQLYANGGAVWSPPVLEMVPFVLWNVQTRRQDAILKCNNNHVQSVCFSPDGTTLASGSDEKHIKLWNLMTRKFKFKLCGHTSLVNSFCFSSDSATLASGSDGKSIRIWHVQTGQQIAKLEGHDSCVNSVCFSPDGTLLASYRGILNSGQDCYIRLWDFKTGYQILDLDKNLKDILAQLKLPHQVNSLFHNTTTPITILLISQKLIFQAEGALIFNGYFMNQLGIDLGLILKQKGCYLLEGQNQFLEKQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYE9</accession>
    <name>A0A8S1NYE9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0900175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000093">
        <property type="protein sequence ID" value="CAD8092414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYE9"/>
    <dbReference type="OMA" id="KALYDCA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20540">
        <property type="entry name" value="CYCLIN_CCNY_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14248">
        <property type="entry name" value="CYCLIN Y, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8092414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="257" mass="29995" checksum="C09E2697C10B4E34" modified="2022-10-12" version="1">MINNKQKDRYSNTTSSMYIKSTISNPNVKSIIQAVSTILHSQMQEDQEQGKQIPKTSELYFFSEEKYIEEKPEEFDEQRKLLLREPPSVDNIYEFMKALYDCAQFSPECCIICLVYINRLIAFTGLTLNPTNWRPLLLSSLLVAQKVWDDKYLSNADFAFIYPFFTTQEINKLEAKFLELLQYNVTVKGDLYAKYYFELRALFKGDQEFPLYPLDVTQSASLEARSADVQKQEKEKAEKLSLTYNDRQTRKPAAIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZI8</accession>
    <name>A0A8S1NZI8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0980065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000101">
        <property type="protein sequence ID" value="CAD8095223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZI8"/>
    <dbReference type="OMA" id="QKSTYSY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="312"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="389"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8095223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="450" mass="52952" checksum="1FFA5E1C24CF5614" modified="2022-10-12" version="1">MTDNDNQVIQFEEDDQEQEDNLQQKSGKEQNNEQLNNYDQNYNQGQQPDSNDFKPLESEKQPIQVVPEAEEGFLNRIKGCSCRRQTDEKTDENNEGCIAKLDRFIQKYFTPTYLLLVYYSVFSTALLFSLKLIIQDQDLYHAEILGGLFLFVQLLKGLLIYFLNDEWLDELPYLDSSFENGWRQFKITFFIITNLDICYWIYYIYVQNKKDSTIEHMMTLCRMLDALLFNFPLMIVFFYLQMQDLENIQIPYLVIAGLNLISIVNGFMHLNFFAGSSFLSIRWSIQFICELYVKLLLVVSVYIKLQEFNIQYEYFGLFCLCGLIQFYLFSDEQDFTGQILSIIYFQFKTFFVIIFKEESFGMSDLDCDAQPGILNVVVQKSTYSYIQKAFNFCQIMSLAFIFWLFGGWDIVITSQILLHMYLVGLGCLGIIMLNLLYILLSGWKKNMLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P257</accession>
    <name>A0A8S1P257_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cation efflux protein transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T1030130</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050681">
        <property type="entry name" value="CDF/SLC30A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562">
        <property type="entry name" value="CATION EFFLUX PROTEIN/ ZINC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562:SF17">
        <property type="entry name" value="RE54080P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="8">
        <location>
            <begin position="61"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="193"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8097235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="295" mass="33398" checksum="5ECA1FEEE6DD1D56" modified="2022-10-12" version="1">MNKEEEPIQEPLINDGNQQTKGKSNRGMSQLSALTEQSKNETHLYEIPDLSNVTQNVLCRLIAVSVVCIVFLIAEVVGGLWAQSLAILSDAAHMFSDMSGFFISIFSIWLSQRPASQKMSFGYHRAEVIGALASIVLIWGLTILLLYEATERMINKSLVTEPLIMLITAGFGLFCNLVMAKVLHSSPTGGHDHGNIMHQCSGHHHGHDHNHEHNHEHEHDHHEGEKKKQKKKYHVKQKRSMVSENGVSMLVVEQSIESXKIIHNHIRNNIFSIFNFKIGIYVNIYDYNQLSLQFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P5P6</accession>
    <name>A0A8S1P5P6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1060168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000109">
        <property type="protein sequence ID" value="CAD8098321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P5P6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="218"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="260"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="301"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="343"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="391"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="433"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="474"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8098321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="592" mass="66455" checksum="23348D6433F32E47" modified="2022-10-12" version="1">MYCYFAQKKDLYEIFAYAKDVDEEIFSIILQKFRKEKMQDILGYLSNDGNQRLLEQYILQGQNLTQVDKEQKLYFVRNNIEQLINALKKIKDHDFNKIDYSTEENQEYRQDLVKRIKDDMQIIEFLKYLVHLISIENNLIECESNSFNLLVEMKVDMKNKNFENIKIQNASLVGDNFCGCNLSKSQLFKLDISGINLNGALLINCKWNNLRILELNKLDGHKSPIASLCFSPDCKTLASGSYDGLISLWDVKTGQYIAKLNGHSNTAYAVSFSPDGTTFAGSQNNVICLWDIKSRKIKSKFEGHSGNVSSICFSPDCRTLASCSWDSSIRLWDVQTGQLKAYLNDHYDLVFSISISPDGTTLASGGGGLVGGGDFSIRLWDVKTGQQKAKLNGHSNTVYAVSFSPDGTTLASGSRDNSICVWDVQTGQQKAKLNGHTHYVQSVCFSSDGILASGSWDLSIRLWDVKTGQQKAKLDVHTHCVQSVCFSSDGTLASGSWDKSIRLWNVKLGEEIKPPCKNYLDISLQKNLQSDGFIYNITIPLISESLLLQAKGALIKGIYINHQEINLLELFNKKGSLILEDSLNITQNAIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P764</accession>
    <name>A0A8S1P764_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leucine-rich repeat-containing protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1070188</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P764"/>
    <dbReference type="OMA" id="AWENERI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052116">
        <property type="entry name" value="Centro_Cilium_Assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23170:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23170">
        <property type="entry name" value="NY-REN-58 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="335"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="432"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8098733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="577" mass="67683" checksum="56FD822C89F61966" modified="2022-10-12" version="1">MSVSLQTMLEQLKNAQRIDLSEMYIGDDGARLLSNYVKNNKNLKCLVLRGNNITAQGFLDIVTALRGCPLLKILSLEWNQIGSDGVGIETLAGYLVSNKSLQHIGLSNNKLTGDQIEPLANALRQNTTLLSLDLRWNSLGKKGGEFLLSAVGDNKAILYLDLNGTEIIQQDHEAIEDILEQHRASNPIQKDRILSIDEDVVEEEEPMISGLAGASVVQHLEQMLEQERIHTMHIKQRMERELDELMRRDKADTRLLEELDAKTRQLEADNRASLYQIEKLRDEYQLMERQQEDVTRSMEEKIRQNEFILNELEKKHRTNLDRALNENNFRARDLVRDWENRCRAVEDRSRQLEQENRILEEELRSLQERQIQLQLTYDQELRDLGRRIQEEEYQKYHIVSTTLDAKIRAMEEARDLIIKRNADAAREYDERILNGKQEVQVLYDELERLKNQNKDFIRQNQELRQKCDSLKQQAKVKESAVEKYESEINTLNAQIEQKRKMQSDSLNQLLNEQKIDRQAWENERIQQTERIHSLEKALRESHAENTRLRQEYQRLGELLQFNVSNALHQAFQDNGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PED3</accession>
    <name>A0A8S1PED3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1150110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000118">
        <property type="protein sequence ID" value="CAD8101435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PED3"/>
    <dbReference type="OMA" id="MNDNPYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="146"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8101435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="233" mass="27526" checksum="8390E031056D2BDB" modified="2022-10-12" version="1">MNSTQQPSNTTPNQMRQHSKSFSVSATQKLSQQQKKIERSYEEIIAEKQLSNVEQQRLTSVFNYLLTFNNEKIKLSEPGITAETLMNALEKVDYILPIKEAELFIWEIDEDGDKVIQFYEFQMMYKRCIFDPTLLEPRNLFNIVQFFMYDKAKHGKITVEDTLELLFVRHGRGGLDEEIQAIFGREEKNSDGTEKTLSFTEYYEKTRIRDFQRRKEAEQARKEVKLPLLDDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PFX2</accession>
    <name>A0A8S1PFX2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1170117</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PFX2"/>
    <dbReference type="OMA" id="INASKHI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="299"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="572"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8102077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="704" mass="82299" checksum="BED7D761CFDAF50C" modified="2022-10-12" version="1">MIQILLTYTLHIEIQIPQFGFAHSLFYAYLMFKNQCNLWILFLNLLFIGSYCIFRMYDHLDLQIIWTVIHYAAIYIILTIMNYDAFKQQQSPKLIEIFQNSERYKWKEMSQHELFQDNYTKNLCFNQIREGVILYEQGYEKQPTFINRSAKSMFQISDETQIQTAYQNYFKIEKVETKKSMQSIKSIGIDIQNSIKQYSGSLSFNSKSQYISQQGLKEMTLQQMLDKIWIKQNKESYLFYVQQSKHLSQSQQAPILEVSVYVNSQIKKVMTIICRDLSYKKYIKQLQSHYNQKSKMISFVSHEFRAPLGCMITMLEHVAKEQNNLYIQNSIDNSRYLLNLCNDLLDLAQIQANKFQLKMERFNLKLLCQECLQMFNLQAEKKQLKLFLQYQSVCPDIIEQDQNRIKQVMINLIGNAFKFTQEGYISLKVELLDDYVIEISVSDSGIGVQEQDKEMLMKAFGKINSEESKKLNAQGVGLGLVISNKIAQQLGDGLRFESIYHKGSCFSFTIHLQDLQSQESKIVRSNNNKQSILSDSEMIENLNEDDESSKEIQIIVTQPKKQINCSIECCTKILIVDDTQFNIDALQMLLIRIGVHQIDYSINGYKAIEKVKLKEKCNYCNSNMYGLIFMDLEMLGINGINASKHIFSYCEEKDLKSPIIVACSAYQKEIEFPKCQQVGMKFYLEKPVEIRQLQSIIDYYRSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PH93</accession>
    <name>A0A8S1PH93_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1180187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PH93"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8102592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="113" mass="13483" checksum="976F69C73BFE5B1B" modified="2022-10-12" version="1">MKQYDKALEDVNQSISINNNQAESYYQRATIYWRKSIFDPALQDCKKCVEIDTNFAMGFCRISFTKLGKKSKMKGQTQLQLFFKECRFFLQNPVLHMEQKKYSFNCQFNIFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHJ3</accession>
    <name>A0A8S1PHJ3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1180011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHJ3"/>
    <dbReference type="OMA" id="FIDHRPQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8102261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="275" mass="31917" checksum="E23A4089468CB7FB" modified="2022-10-12" version="1">MQSQQAWHGLKSCKFNYSEKIHIKPQDCITDFAGGEIQQPLGRVHFDPKFGDLYTWVPHLKTNIPRPGDGKSQEFPPGKQFIEHHPQEKKPRAQRLHLKEGQSGHLEDVPYGIKTFPPIHCEKRHEIEEMMGKKQRLEYLYQMRNGLPVASLGDKIYKNPEYASDFFKEGGLITGSSNIQRKVNINQLHEKEMEKKIQLIQSKIKKGTLWSDKVKMDQDAEKKQDIEDLDTWIESTLKPSNPNYQDPDKFFENLEKQQAQDPKKAQANQKKAGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLG3</accession>
    <name>A0A8S1PLG3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1210028</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PLG3"/>
    <dbReference type="OMA" id="FRIDCSF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035860205" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="34"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8103398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="246" mass="28508" checksum="644DF93774D4E7AC" modified="2022-10-12" version="1">MKFVLIVFLAFVYAKVGCDTDNKERVKERGMSKKKEDELLELHNEHRNDVAVGKQKNWQGKFQTASNMNYVKWDNQLARFAQDCADKCPANFRIDCSFPPQYGFVAYLGDVENGGADWTAKRVFKKWAQHEDHARQIEMAQVQFFGCGRSQVSRKNGMSDEIVVCVYNRKPNLHGDVYKAGVAGQECLHGRKKGYLGLCKHSATDMSVLNFKHQKKNNEYKFHQQEVKHHKGHKHHKNNEGHKKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRK5</accession>
    <name>A0A8S1PRK5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1280093</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRK5"/>
    <dbReference type="OMA" id="DYGVEVH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006742">
        <property type="term" value="P:NADP+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04694">
        <property type="entry name" value="NUDIX_Nudt17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050241">
        <property type="entry name" value="NAD-cap_RNA_hydrolase_NudC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033716">
        <property type="entry name" value="Nudt17_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904:SF1">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE-LINKED MOIETY X MOTIF 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904">
        <property type="entry name" value="NUDIX HYDROLASE, NUDC SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="6">
        <location>
            <begin position="78"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8105756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="248" mass="28811" checksum="421CA51EA46C01D2" modified="2022-10-12" version="1">MQKIDYGVEVHIDDTLYAGRCLLQILKSPLISYKYQQDQAKFLLGIDQQGWPQTQLLHGTNCPCFNQDYQNKDMKLSKIRLATVAMLCDKNDNFLITRRHSQMKTFPKTWVFPGGMVERLQDLESECLREVQEETGINVLPILNKMELKVLYESVYPTQLQVGQFPQKQTLCIFYEVKLNESCNNIKVKIQESEVDDFKWIPKNQLLDIMNVSTNDQQYKEMSGIYPNQYGSGIGEGHIKAFMNSYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSV9</accession>
    <name>A0A8S1PSV9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1290088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KYFHILE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="968"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1166"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1286"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1535"/>
            <end position="1552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8106074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1597" mass="189854" checksum="85C8A696EF596ABC" modified="2022-10-12" version="1">MQQYIKEIFHLRLAELLDHLYFHDAKPSINIMIVIIFLMQKLYLLFWPQNSHREYSDEVYLNISGSLFSWITFQKPLEMINSNLIYNLISIFYTILHLTMFVVFSFSLYNWQIVSKIFTFYISYYQLIILYHTEIHHLKQQNDLIVILLLGIPLYINYLIIVYCSRNYFIISQQHMLKRYSLYNYLIVIADFLYFITYSLCSDYLSKYLLLLLCIVYFIDAMIMQPYCNKINLIYIGATGVLIVSVIVRIVLWNESLYSQFYGIMLLVPLFSHIFMQIYQIISEDVKQQKLSMSLILNLGEIINHNNSNSIQKGIGNYLIKQYIDVEILNTQSILQLLEHYMKNLEKNDQWEEIQLYRILLKHEIQGTYLQSLLETKRFQIQNKNHSLFFKTIGFLISKKYDQQIQDLYSGESISNQHYHYQIIKIKEADNLFQSSLDIFSDLLDQKIQIWIQLQKGYDKIEHTAEDLTKMYKILNKLKIKMIQMIQTTDMESILKVQTLKKFNIVELRFLSLYFSFVANDYQQTKIIEIYIEDLIKQENNSSNSSILNINIMNNDLIVICSSILEKTGQIVKANIEEISRFFGYEDKNKKQDLHLINLYMPKFISQDHDQYVYKFINKGNSSLYNKGKLVFCKDYEGFIFPIILSFLHINEKSEDFILTTALKKVQSNQDYILFDQNGKILGISKYIYSILMNTPTINSSTQSIYSSIDQSIYECYIQFWIPNIDSLIKSSNFDHENYSINKVIIEISILTNFIQYLYYFKQYQNINPQVYKENSYFEEFLQNIKQQLSNHLILNCKIQFEVQIQIHQLPTGQYYYICSMSKQYKEAQSIPSIDKKTSTYSQFQLIQQQQVKIFTHSTLPKFITDLEKITEIAPQTNRIYENQMLIKDLNQEQKQELLFSNVESQQIINQINSSSQIEKQKKNDLIEFDDQKISQSSRYSRNSNFQVLEQIREYQKNKEFCVSIKKIWIVTIFLYIIIFLLMLVILIVLSQLNDLLQYDIQLVRIPDKFNRLYCSFATMGQMDLERSLLGKDYGQYLNYRIINQASTIRNEMETMMINLKNRFSILENENRLQNLTVRILNEFSYFEEELSMIQFDMIADTYTQSLFQYINQALNYSASNHLYLIDEYYKLQFIKANLINQITSSQNLIDQIIVEIINQQENTNVVLNILLCVKLCFVIGCIFCLIQLWKQPFKIVQLQIQLLSHLTEQQIKISINTAKQAKSIINHPYRWKRTNYMNECYHKNIQKREQDILNKIENFTKKKTKQNLKLFDYQFNHIGVYLKNSMYLLFFLSFILSSFFYMKNGIDSSQSEIQLTIQYVQFKQDLDSLMILTQLLKTNSILSEKVRELGFLDLNPQLLDTNKYFHILEQELLAQFQLKYQKAQEINEIIFQDIVNSKKISQIDKEILQTLYQDDLCSVMNDQLPFCNFTNNQFFYFPDFPKPKDEDNNREIFRNGINGIFSKLIAILKSYYQLELQGQINTNKTETEYFLSTLEFKHYILEYFFDINKAVVKFYVTILESTTKILQNDFQNTLFFYLIFGFSVLVAQGTLQITNLSKIQELVSASKFTFILMPLESLNETKCLAIIKQIVKQQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTC8</accession>
    <name>A0A8S1PTC8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1300043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTC8"/>
    <dbReference type="OMA" id="DAHQINK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="229" mass="27110" checksum="D898BBAB5B0003D3" modified="2022-10-12" version="1">MANQLDTISFSTQQRLHQYNPSTDSHKSNKQHQYPQNFNIQQINKEIQFIADYALRLIKTTQSTSDKYKYIIRIAEISMKAKEVDINKKLLDDVVEELTIIVTHSLKSKTVQLLLTRLIQLYKKKQASFINSEKNIKKFASYFNNKPKNQNKIITNQIDSPRLNAQIQKSYSPTSHYKNSRITTSKEQKLQIQNHHLEDPYNLNYEHLKALLSPQINFQRQRNYYLSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PTS0</accession>
    <name>A0A8S1PTS0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1300123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000133">
        <property type="protein sequence ID" value="CAD8106442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PTS0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070224">
        <property type="term" value="F:sulfide:quinone oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070221">
        <property type="term" value="P:sulfide oxidation, using sulfide:quinone oxidoreductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000593">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015904">
        <property type="entry name" value="Sulphide_quinone_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632:SF2">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="54630" checksum="1C63B62278971B96" modified="2022-10-12" version="1">MLISGMQLRHSLTPRYFAGVFNKRKPTKKDLDHYDVVIIGCNLGGVLSRQLDKVSHGKYKTMVVLDQNINQITPIRQIYEQQKTAKTDFLLNAKLSLNMYTAHSDQVGCQTILPEENAIILRNGRRIGYGQLVVAMGQQVNYDAIKGFEEAWQDFDSPVFTNLDHPSWRSSNHKYTRWHYNFNHGEAYFCIPQFPFSGEVESYNFLLSQRIWEWQTANGRQSPIKRLTIVQPNERFVQYNDAGDQFFKEEIKKRNINVEYGLKLVEVNKQQNTAVFEDVKTGNRQTRPFNHLYAVAPTKPHDQLVKAGLTTQKGLLDVNIKTLQHNKYKNIFGLGDVNDVPTTNCFWAGFHQLHVVRNNIERNIAGKSLNAEYDGYSKVPILLGQNTLTFLCHKYNNENAWHNLYFSNGGFLAALRYYNWCKNFKKAFIDIYLEKNWGPPYYKIKKSFKLPEGESNDHGFLAKLLPGKKEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PVT0</accession>
    <name>A0A8S1PVT0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1330138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PVT0"/>
    <dbReference type="OMA" id="WTSIIYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF137">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="8"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="155"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8107377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="291" mass="32602" checksum="CC19473B0E9B4BE3" modified="2022-10-12" version="1">MQIQTAAIIKQIVSSLFAAVASMLLKIVSDLDFNQTLYLRAFIGLILLASASIYYKLQVYNFDKQTMDSLINRGIIGGFGAFIYFKGLNLVLVSESILLNRMSPLWTSIIYIIVLQKEQFNRRLLINMTLSGLGIFLIAKNNNQTQITVDSYYHWIGIILILVASITQAIVNILVKSVNKQVDSIVITLYQAFFAINLPVINSLFDGTNFKIPSFNGTFMIVIASVISLLAGILQVQSMREGKLSVVSNVSQIQLFFGYLIDFFVFSAKFNQEQIVGNILLLCSLIPLIWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PWA0</accession>
    <name>A0A8S1PWA0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1340044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PWA0"/>
    <dbReference type="OMA" id="EYWERLI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="357"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="396"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8107557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="535" mass="63696" checksum="1BB6AE37DDEE3538" modified="2022-10-12" version="1">MNPTFKQSQQLENNSNIMGSSAGFNRKIKLTSQRPGSRDQKLKTGEIFPQFQDQLATNPFNLLSINLYQDENTFITTLKAELLQIQEEINKLDIDGRIRNNDPRQEYRLQQVKKWVAQYSSSKFTLEKLNNIDISQLLTVLDVRTLEQAVVKLYKFISIMDNLIMKITEYEAVHLIKFDELFTSLCPNHQENLQSISVLKFPVSIANLNRMTELYQVKQDRNQPQKIINDNSNNQIFLKQIKQKDDQIIELNTTIKQLQLQLQVSMKGNNNNNNNNNNNNNNNNQHSIEQILQIKNEYEIKLQKQSEILTQKNDKEIEILQSQIEQFQKQLIQLKNYYEKQIADLIQQNQQEQLNTKSQYEDKLQQQQQQQSSSSQIQTQTQIQSNNVEKDHNYYQKILDKAQQEYNERYQNLENDLIKLRNQLLEKQTIIDSLQREVQQWKETADRLNKVKDEIYQQFQKRYTTEEQFSNSYEAALKLEFETMKKAFEQKIQKYQQDLDQQRRETGKQLNEIRMQLEREQETKKLLLNKLSLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PXZ8</accession>
    <name>A0A8S1PXZ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1330144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000136">
        <property type="protein sequence ID" value="CAD8107389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PXZ8"/>
    <dbReference type="OMA" id="NTSLHAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8107389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="195" mass="22043" checksum="3D9D1CF2110FB99F" modified="2022-10-12" version="1">MINRPPVPKFSDECSTPKRSSDLLEEVQHLVFKMNLDDAIEKKAIAILNNLNIPNTSLHAQALIHCAMNELNQPLPKANVKVEYLSKCIQNQYSSLISTLCQKLKLNSKATKVCCILLQQMQPLINKLPKQLQNAISVKIATDIIYLKQGGINAKIIAQMANIKVEQFQINLNRIKPFAFKIIQDLFNYISNNSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PZ03</accession>
    <name>A0A8S1PZ03_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1340133</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PZ03"/>
    <dbReference type="OMA" id="MEINNRC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="23"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="392"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8107733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="737" mass="87727" checksum="9D5C2AFD47D00FA1" modified="2022-10-12" version="1">MQYNKLVNTLLDVPTKEYPKRRFLLTKLLGCGAEGGAFLASASDWGNNPQQVVIKIQKNMKPNEKQFLSCLLEYQNLYENGNNQQYLPTYLIRIYENFQWQQNHCIVMEVGQEDLYSFINKSRNVSIQEKEQICFQIIYPILFLHQQKLIHRDIKPENYIKVGNVFKLIDFGLIRSSLSDDKTIQVGSTIFQAPEIIENRSDYTDKVDIWSLGCVFYEILATKPLFDGQTYQEVTNNIKSHKNYPVPVNNKINQLQISQQFKTILSSMLIYDEKKRPSIQQVYDQFKSYLNPQQKVIPQIIPKPDIPQQKQQQESNQQQQIKLQNTNCANKSENNSSEQQNQIIQNKELNQNQQQPKIDDVIKKDQIKSEEFNKSQLIQIQKIVDSMVESKLKETQNQLIQREQEIKQLNQKISDITNVMNKKQQEEQVNQNKINQINQGKFKEIESQVQSIQQQKSNNEKYNQDEKFQQFQSEIMIVQQQLKQIQQQQSLQNIEQQSQQKTQEIIQENNKLFKEQEVKLQEFLNKNIQEQNQQLTIKLDEIQKEFNKFKEQQMEINNRCLNIEQDNQKKQQEIQNITVQCEGINKTIYEIKIQQENLQNSQVKETQLNLSNQNRIDEKDKQISDIQNKQCILTNKNEDFEYHIFFILIQINKPTLSTQYKNNQYVLLEKFQIFQFLMLHYIRQFQINITLIKEFQFNINMNSVRVDTFILSINLNLFYKFQRLKTHENKIDNKEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5X8</accession>
    <name>A0A8S1Q5X8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1460027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8110962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5X8"/>
    <dbReference type="OMA" id="AYFTQIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="164"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="240"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="386"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8110962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="489" mass="57278" checksum="B8CF1F75A3805198" modified="2022-10-12" version="1">MENQRIIKSIKSPSSLNSKQSTKYIQQPNVLFQQHPTQIVKKSAQQNIPPIPLKQVNNILQTRETPIFKQSQDERYSYTKDATCLMAKLDSLREIKSADSPIRNHVSPIPSTLQSVVVLSQNNAVKPQLLQQVACSSCVEPFKIYNNNKNVNQSQQLSQTLPPKKNYESMIQTLMNEQELLVKQYNDKIKLLEQKNQELSQENNNLVEQNKLFLDQNLQLKNEVTQFQLKQQQTNVIYSNEDTKKTVQFLEHELEQKKEELNQLIQNMNEILSINQKTEEKNNELSQQINIILEQKEKEINAYFTQISMYKQTIQDLEQKIVQLISNDSLQQQKLKEAQTKIIQIEQSDKNKQNSQQQQQQQKYNVKETFEYKQLVLELDSKCVTITEKENQIETLKIKNQMFQQQLAQQQQCQISIKDHQDVLIQKERENDDLKSNLINKDEEIERLNQQLKFVRKEKAKLSINLMNAGMANLVMLSQGQYQQDEITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q7L8</accession>
    <name>A0A8S1Q7L8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1470015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000151">
        <property type="protein sequence ID" value="CAD8111263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q7L8"/>
    <dbReference type="OMA" id="DSPIFCT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8111263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="298" mass="34925" checksum="14C3FA75904865A8" modified="2022-10-12" version="1">MFESSVDSEFKGPIPPLLSSRVGFQEGDLQQNSSCSLNQAIPLPLIRQKTLGVKKSEYQSAYDNEEIQYGSKPQFLKTIIAKSLQSNFINNLWNRSYLRKLNQLSPFQIQQLDDLQISSDGNEQSRGYQLWILVDVFTPYSKFIAFWDAFQIITYLLIFFWLPYKISFEIYHISELFSETKSAIIEYMLMIIMALDIGVGMNLAFIEKGQIIKDRKRIMLNYFNKNAFVDLVKFYIYLCGFFFYCDSPIFCTQSQFQRLNNDLDLNNLMCCILYIEDNQNKQDSSLNLRVFQSKWVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBL3</accession>
    <name>A0A8S1QBL3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1540015</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IFT57 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBL3"/>
    <dbReference type="OMA" id="MSEMCTE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019530">
        <property type="entry name" value="Intra-flagellar_transport_57"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16011">
        <property type="entry name" value="IFT57/HIPPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16011:SF0">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 57 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10498">
        <property type="entry name" value="IFT57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="141"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="270"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8113062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="394" mass="45989" checksum="C2812D8B511B5AC3" modified="2022-10-12" version="1">MSEQQAGQDQLDVTAMMDELYDKLILLNYESTYLKQKGGKPLNRAYFVNQTNSSEQFTQFKTLVKWLFQQNDVQTSDFNKLDDPVTLSQNIINELKNIGIEVDFPPLKLKQGFGEYVVYVLLQLATKALQKKKFQYKKAKIEQQSQTRQDEEPVQETGSVSSDSDPEVASDEEPEDVFTEQGFQKDEDKMVIESNVNPKEWAKEVERAAQKIKIVIKPDAGEWRQHFDATKQYSNQIKTILPEARIKLERMSDELGEILDRITKREYNINENMSDMCNEFKKKNEEYKKIELQYQNYTNAKKEMNDQYKQIQEKFETVQNKLNEHGSVSTDQSPVIRIKASLTKLRLEIKQMDLRIGVLSHTILQRTFHDAKAVQERDYHENGLILNESDELTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF17</accession>
    <name>A0A8S1QF17_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1560012</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000161">
        <property type="protein sequence ID" value="CAD8113611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="QQKVIEC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004215">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004818">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103:SF2">
        <property type="entry name" value="MIDASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103">
        <property type="entry name" value="MIDASIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="1023"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="1674"/>
            <end position="1833"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="2005"/>
            <end position="2179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="4100"/>
            <end position="4227"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1386"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="4100"/>
            <end position="4126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="4128"/>
            <end position="4148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="4149"/>
            <end position="4177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="4187"/>
            <end position="4227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8113611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="4245" mass="498105" checksum="87FEEA2DB79CED54" modified="2022-10-12" version="1">[encoded data, 4245 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QLB5</accession>
    <name>A0A8S1QLB5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1730024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000182">
        <property type="protein sequence ID" value="CAD8116493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QLB5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="177" mass="21095" checksum="9DF8D42744DA0989" modified="2022-10-12" version="1">MMKKMNKQYNQLIMHYQLILVINCYYIRKVQKLQINILADSLSLIIFKLLNVRMLNHYREAIIWTDKALRVDSTHVDSLYTNNKQIVQLPKQQQANQALSIDSSHLDFLNTKAESLKQFHIRMIDEYEEAIKWADKDLSIKSWHVSLLHTKRIQIINNYLADSSKQFNHKLFDYKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QNM7</accession>
    <name>A0A8S1QNM7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1810017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000190">
        <property type="protein sequence ID" value="CAD8116801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QNM7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="69" mass="8350" checksum="D692F36802ED0872" modified="2022-10-12" version="1">MRLMICIDHIKSKSFYASMILLLANQRGMLNQLLIIAPTFNLRISDKKYELYFFIYIFLQKNFNREKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QRR9</accession>
    <name>A0A8S1QRR9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2400005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000249">
        <property type="protein sequence ID" value="CAD8118449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QRR9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="176"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8118449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="483" mass="57468" checksum="B69E8BA7D83F5BF8" modified="2022-10-12" version="1">MTRLQFYLINLKIKSQLKLINQYQNTFKERENLMVLKDFLMNEKNDKIHFTFGSYLQFKLGSMDLQKKNVNPQTICKIQQFFDFIISNKLLVLIQDNDERLGVIVKNYKKFFEKISILISQQNKPHIMPNLKIQFSISKNISKTLLSERKQYCEKEQLEQKKLNEINLFQQLKYLMRLLEQIIQEAIQDINDNRNEPYEGIMLQQEIEKIQKKLSNLNLKNKEFSVVNKLKQKLLALSSATLNKRSFQKEAECVLKLILQTEKKASELEVLLIQKENLIQSKNFKKNFDEFIESFKAILILQDEIESVSQPEKLKEIKLQCSYKLSFQCMQKQRLNMQKLKLSSEEFQQLKDVIKLDEFLHDIIIHYPQKLIACHNQYQKYILPELITMKVPEEGLQNNLSKYQGTIEYLIFKLSIIDEQIIQSEKKDLELIQKEFEELLIEEFRSIQLIERIMKMIGDISIDESIKNVANEKFNQSQLIIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QUU7</accession>
    <name>A0A8S1QUU7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BD-FAE-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T2950002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000304">
        <property type="protein sequence ID" value="CAD8119183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QUU7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049492">
        <property type="entry name" value="BD-FAE-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF33">
        <property type="entry name" value="KYNURENINE FORMAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20434">
        <property type="entry name" value="BD-FAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BD-FAE-like" evidence="2">
        <location>
            <begin position="56"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8119183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="316" mass="36505" checksum="8ADF401BBA973F64" modified="2022-10-12" version="1">MQILQNLQRLTTQAKLRYQEFRLYDAASYMLNHLTPRQSFELNQDIAYGTKARQLLDIYRTTHPLPQRPLIVFVHGGAWQHGDKKDYVFIGEAFTREGYDVVVLNYHLAPQHIFPSYIDDLTLALNYLDQHQQELYISTEQLILMGHSAGAFNVMSAIYHPTPYTLECRSKIKAVIGIAGPYHFDYKGDPIAQDAFDQNIPYHEVMPYYFVDHNPIRHCLVVAENDQVVKKSNSDDLDQQLRQCGNYSQIVMIPKTGHITVLGSLSSLFSWHFQTKQSILNFLDEALKASQLAFFNIQQLAFHEVRAQSWNGQYCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QVG8</accession>
    <name>A0A8S1QVG8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2720001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000281">
        <property type="protein sequence ID" value="CAD8118915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="HESDVFC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="248"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="278"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="327"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="373"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8118915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="518" mass="60721" checksum="B0C6E78A955021B2" modified="2022-10-12" version="1" fragment="single">MKNCKTIETQEMLQCSNKHNNCPVIVILLDKDLEGAQRLVCQQCIVNNLGFINGISITQGLCRIQSIKEQIFDSISIHFQLAYDKLESFLGQIQQVKRDFLQSINQITENLDLWMKQLVTLKEGYEQYSLLAEIDILTKPSDQLIELEKQKFKDKIKTIIDQYWTKIFQNIQILERTSKDQELLFQLNQLQDLNLETIQSAENVQNEQQQKESNQSTISQVDKQNRVQSQLIQEVNQQQQCHVLDFNNDNSLIAASCDKNIKIWNFRDGQMIDQNIVLTGHESDVFCILFSKKINWIVSGDKNSQIRSWIQKQGSFGATQWESSQPLTQHKSFILSLYLNQNEDELISGSGDQTIKVWRVNCQSNKIEFLYSLCKHNGEVDKVNLNSKETQMVSCGWDSQIILWEKDGSNKWQFKYILTQSIQDIGMRIGYLEDDTIVWCQLKQPFLHVFKLQNGKFQERSDLKVQLKEIIPENNFFDYNLFQLKYLSKEKVLIFKSYRFLYFMKESLNSQLIYLCXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JLK7</accession>
    <name>A0A8S1JLK7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JLK7"/>
    <dbReference type="OMA" id="KTHFNAK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035814151" evidence="1">
        <location>
            <begin position="18"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8042863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="325" mass="39205" checksum="62BAB4493321560B" modified="2022-10-12" version="1">MLLIILLLINAFAFVHKQEQYLMDLYNKMNAIKEFLSQLEYYTALEKLEIRSKENLEFEEESLVIYKEHYQDFINSFKLQQTFNKNEQEDIKLFLRETFPEVVEKIKRIIADKEKLIEGDIKELAKLKRQQQQEDISQRFEELNYLHKKIQEVLEQNDEQIIYKTLELLESKIMIGSFKQIQSQEVDFFMKSVDKLFREKQNSNKKQEAIQLLSVMSSVISEYLIKMRNDQIESSFDLLDKELFFTESKNTHLNAKEQYINLLSRLDQFHQTIWIQMMDLPQNSYQLEIAEKQYEQALQIGNRAISYHTQMNEMIKAMIERFLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPJ7</accession>
    <name>A0A8S1JPJ7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0060496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPJ7"/>
    <dbReference type="OMA" id="ENQDQYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8044412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="168" mass="19806" checksum="13630554B8B9A83D" modified="2022-10-12" version="1">MNFFHPLLQTLQFMRFNLSADRQQTTLNLLNPQIKVEENDPLLPPIYQMTQQELLQRHIVDSTPQGLQMQTLPQQQDQPKTINKKISKKTNTCGHPDKEHYAKGMCNNCYHRLGRNKQPWLCSHKKLYACGLCQNCYINQYNKKRRVENQDQYESELNNQSSIPKTDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPV8</accession>
    <name>A0A8S1JPV8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPV8"/>
    <dbReference type="OMA" id="TREVNWI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF15">
        <property type="entry name" value="NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="254" mass="28714" checksum="A302643D95B2081C" modified="2022-10-12" version="1">MLKPLSGKVITVIGSSGYVGSNVIKNALQYGAIVNGVSRSGQPTTQQNWTREVNWIKGDAMKAHEFKDILQKSDIVIHTIGTLIDSSVLNNKKPGDQGTYEQMNRDTALNVVKELINTNVKFVYVSGSAHPPFLKRYLTTKQEVEQHILSLYQQQQLNPIIVRPGFIYSLTQRWWSVPLKYDLAIWKCVHDNASRLIPQQSFIGKIFNEFKVDTSIDLQDVVDACLAPLKIDLIGKSLNNQEMEKVAQQFRNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JS07</accession>
    <name>A0A8S1JS07_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0090416</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JS07"/>
    <dbReference type="OMA" id="EDYDQMC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="176" mass="20757" checksum="8877E22E5395DC23" modified="2022-10-12" version="1">MGSHCSQTVQNNTVNYEKKHEIDNIIQDKEIQEPKFTTKLNLNVEIDKGVYSMRPCNVNDSVLDITTERNLQESIQQNQFLSKKYFDDTMTINKSHNLVYSNMGLNILSDLCQNEVVSQKQEKQKKVEEYDEDYDQMCKYDVDQFKFQQPAVMKYVDKQLPSQQKTNIVEQRNLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSD0</accession>
    <name>A0A8S1JSD0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSD0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="234" mass="28159" checksum="171910F18C5F6702" modified="2022-10-12" version="1">MKQMREIDFVIQKPERCLIMILDSITVKILSSIMKLKDLIDMGNCNVNHNLSMMHSISQRLQMIQQIGQQMILKFQLIYRKINVIFSYNLPQRLLKQICKQNLANRILQIKEFNHHLYFLDDNAFHFEILQIYIDDQMQLIQLFLPSLRPFQSIKLSTIKNNQFSQLFANCLPRLLDSWDRTDQMVKDEGGELHFLILDRTFKIMIKLDQFQQFYLLYRCHNMNVNRFLIKLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JSX2</accession>
    <name>A0A8S1JSX2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JSX2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="23312" checksum="1917D37A2E46B5AE" modified="2022-10-12" version="1">MLRGLINFKYIFQYPESQICSNHLKSIQKLQKESVQLNVQQILSNQNPISGELTIIFNLFKLMKYNSIYYKLIMFLYIISSKIIYLKWQDHLQFKKQNISFSKKLYNCQINKLIDYSTIQKQSKYLQTEKRIENLLKKLQQTYKTFKRTQSFINIEEDWRKDQKYKKRNIKYQIQNRKTKYQKYQLLHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JUL0</accession>
    <name>A0A8S1JUL0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0060178</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JUL0"/>
    <dbReference type="OMA" id="LYVLHFG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="98"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="198" mass="23792" checksum="A0D32A900EE0C91F" modified="2022-10-12" version="1">MIHEKAQMIFQENHESPMFFSKQTQVSIFLQESVDEYPQISAQNNDYDEINHELNNCYCHELPVLGLKKPRKKRKRQPNYDKKRKRRQSKNQITKLQQSIPFSPQEDKAILLYVLHFGPKFMSIAKYFPTKTINMVKNRYYKILRYQWDSILGESYAYLNQDIESKIEEINHNNCRDETQSFRDKNINLQLLCQSSEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K0C9</accession>
    <name>A0A8S1K0C9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S9 prolyl oligopeptidase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0120359</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K0C9"/>
    <dbReference type="OMA" id="DEVIPHQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="2">
        <location>
            <begin position="149"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8048189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="313" mass="36459" checksum="7551F97530EB3186" modified="2022-10-12" version="1">MNERLKKIVMQFSPQSGQIKQVAKKVILYFGCLFLVVNFVQWKLLYLPSIDGQFSQNNYHQFRSPQERNLPFEDIEIQHDGIILKGWFIKQQNYQTSPTVVFFHENAGNIGWRLEYANQYYNQVKCNFVMVAYRGYNYSTGTPHQNGIQKDAEAITNYVFNNLNIDKTNVYAHGRSLGGAVASYAFYQRQENAKKTGVLQYKGLIIENSFTKVADVVANMNRLFKILAPFIFFNTWKTIDIIPYILNPILFISSGRDEVIPHQQMFSLSEAAKSTKQKFNYHIPLGDHNSNWNYNQIEYFTTIKNFIKQTSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1U7</accession>
    <name>A0A8S1K1U7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0130292</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1U7"/>
    <dbReference type="OMA" id="TSSKEYN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8049179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="666" mass="79347" checksum="B4F773CDA7FE9B15" modified="2022-10-12" version="1">MQQNILSQKKIKYVENIKNHHYYACIPQKGNPNCSDKEKINLQNESSLSQKTISNNSYSHQSNGTYYKKHSIIFRELRSISNQSHNDQIDDIPKAFIFRIKSKNHIQNEPLIIENQEQKEKDRKTKDLAKVLRDMKKKRQEYTQKGQFVPKSPIPLLKHKNNKIQEDLSTEKSPNQVKKLPKIYTKQAPVIIEVLEQYEPLSKSSRLQGNGIGEDPFQVKLTRYYQFLFKFQCNHLFYVKQSQVRSQSYYIEVIDENEIIIKELMRKRWWWCEQIEKTDDVYFFWSKRSNSQFLQKQKIRVLFDESQNKVVDPYNLDNYEEQIKFAIEHKLALISPSGRLHNHIEINQLIGFKKNFIKFLEQYASLLNLDLFQYFPYSINISSQQDPKLNIFLTQQKNQNSLWIVKPGEWANNGLFMKICQNVKQVYDFIISEFKNQDHNFETLVIQKYIKPLLYYKRKFDLYTYLLISQINGVVRVYFYDEVYGRTSSKEYNEQDLDPLIHFTNSPIQKESKGFSLQEPGNKLSIDKLQEFFVQFKLNFKETMIPQLKAIAIHAIKAVFTHLVVKDHNFEIIGLNYIVDQDFKPWLVELNLNPSFIPDCQYHQKVITQMLDNALYLTIDLIYPPPSLWPSNKKQLIDRFNLQNKFQALIDSRFDSEMLIELFEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K4L8</accession>
    <name>A0A8S1K4L8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0140169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K4L8"/>
    <dbReference type="OMA" id="EITTRKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8049797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="658" mass="77195" checksum="AB4C25D0498F9832" modified="2022-10-12" version="1">MQKQRKCENKRTKQREQRKYIYLIQQKTMKLCSIIIQNKRGQNIFVVSSNDYSHELSLQHSKDIYNYILSQIKTGKKIDRSIVLDSNYVCICNVNKDAIFLVIVQRNELFLGYEAIKKLAEFYATYYKQEITTRKYQEMLLLLEEFLYTKEFLPQKLQFNLTVKVPVSSRCIADCDHLQKFSSLQATIEKNLIANIPLKYAKKQQIIEKEIDIINIFIKPITNLDEYKPNQEELQWKQKFKKSLYDCAFKMKSKQVHKENIFQKYKPQKPNTQYKQHAQKSTQLLNQYKGISSSDIQIMKLIFQSQIQKGQQDLSNQQNKQQSSKPIQQQHTRQINLLDYGENFTSVEAPKILPQQVQSSNEEQPKTNPIQVKPKLQQNANDLLDLDDRPHHQVQPQIQQISGPDLSFLNLSPVKQLEPCLKMLEKMIIAQKDGRLKELKIYGQIALDGDFTFDDQSNLQLLGQYWQDTYGCQRRITPNSLDLQLLNENSYKMIVKNASYKVPKGIIDYIIPTKAFSQQRIPVIFLYRLECVQSFASYCLQYKVNDQWQVPLQDIVIEVNLEKDVEYDDLKTIPVAQNITDKQITWKAKQLLQQQKGKLILNLTGIKKQGSQILNHIRISLKANTSVIENMDSIMFYNSQEYKCQKKLSVEYKIYPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K672</accession>
    <name>A0A8S1K672_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K672"/>
    <dbReference type="OMA" id="TQQKEEW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="382"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="404" mass="47763" checksum="2B197F88536943ED" modified="2022-10-12" version="1">MGYFKNRPPFQKRVANSSLLSQSDEILLDDLLFLISCFENNHSEFITKAPSYYIEQYFTLTNEQEFANSYIIRNKVLLTCHLMLHEFQLGIDFADHFLNQKFNALLNRKSPVLVKTTQQKEEWLSANIQLSYFQYLQKLAVNIQVFHACKVQQYPLFQEGQKFIDIQTKLLWLFKISNLVNSGLGQMEILKQALQMVPEDMLLKEIVLVYWSDIIVFYNFLSKEILQLLDYYRHLATHICLQFYELYLQLFQIRNSISDLYKYRKHFDRDNVIKQPKWFDVNKPVHKQIEEFMLKQKLNMGNHTDRAAQKPQISSRGSKSQIAFDTRIDLSKLPRQNNTIKTHRAGRQGDSDGDDEDFEQLKQILQNQNIQTKLTMKKQQQKQDSQVSFQKQSQEEGTDIPKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6I0</accession>
    <name>A0A8S1K6I0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0130005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8048618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K6I0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035890506" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="983"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="983" mass="115827" checksum="1440BA89D60A40EC" modified="2022-10-12" version="1">MKQKIKSFLIIFQICLFTVNAQQKGCSSKVDQLQINLRDHLFEGEDLNYDLKEEYLKQSIRLQYREQLNSCDFCNQYQIGKSFSLQVNLIMDELFVFLDQSKTEESIFFQKDKIPTSDFKNMVKIAPVSNGLQCFDVEYIQNNKFIIDCQKEVIDNQITKKTDVFYLYSKQTKDITFFEDEAFIKLYNQRYIGLYYQKSELGNETTQIVRVTLSYSLKPKEKPYQNPILNDQTLLTNYLLDDVDSVILFRRSDLKFNIVDFKISYNGFIYLLDFLQGLVVVKLLQTGEWNLVHKFYEFERKEILFAFDHSQEEKLNYSEDGMIVFLQQNSFSVSYKIFYGTQQLPFIQLDYPSSIKISQDNIIVRNDKKAYFYKYDFENHRFYLNSQLNINSTDILLVNEFESDFILLNTINSYRYTFSNGYLYFPGSDKLQEKKVIVIKVKAPNNQQCVVSLTYQIIEDNESWIYRLDDQSMPFPKSIAEGQAQSIMKKQASGRNLQYKLTKPVQIGGTEITTSIRKRVELIQSPTQPKNVIFAQMISTQDGRIVYQFLQTEDLALQLLRCIYSPIYKDTNQCEVFVQNLDISSRLTRYNCQIWLSNYKFYLIFQVLVKTINLFTIQKQQNQILQTSNQILSIFQYMDYLLIHTANKDIFTFFIYRPELSVQATLKKNSFQQYDYEDWEPKAFFTNKKLNPNILFIQYKKNILILDSNFTLFSFINTIKITPKLDYSISIGKVTFFVVQGKSSNDAKDAQIYEYNFADIDHVYLQKKLYFCWYDISTPLNADFNEETGHLFVRGVCLYSSKSFFLVVKPNTPQHEALDRAWDIADALISDQKQILIASCGLHQTYLYLKVEGEEKLYALYNEAIMIVNSKIKQDIYSDFFSLNLQINNYNFQGKPEKFNTLNISQQVKIFSTLNKLHSIRMTSLFHNLVQKYYQFMIFKLLGWVTNGILDKLEILQLIVNNAKPIFNCKPPFIQSIKKPLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7W4</accession>
    <name>A0A8S1K7W4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0170055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="2">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7W4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0697">Rotamase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8050433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="269" mass="32252" checksum="608E7812580C4C89" modified="2022-10-12" version="1">MNNQFDWSKHQFQQVEKKIITEYDVEKFKKTKIIGQLLEFMQALIKSVQHKKISQTKENQKFLEIQQFFLQLEELLLKVPPIQQSLRYGNIAFRDWLDQIGPIVDNYLKKQLPENLQNAAIELRTYLLDSFGNKERIDYGTGHELQFFLFLFSLFRLGIFNEQDYEGIVRLAFYRYIIFARKVQMTYMLEPAGSHGVGDWMIINFYLLYLELQSQYKMMMAYFLIAQQKRTLLRILKKNICFFNVQISLIMLKKGHFMSIVLFYIVLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KAK6</accession>
    <name>A0A8S1KAK6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KAK6"/>
    <dbReference type="OMA" id="MLETTCG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00374">
        <property type="entry name" value="RNase_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.730.10:FF:000017">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF22">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035733804" evidence="1">
        <location>
            <begin position="18"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8047688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="254" mass="29457" checksum="E3A4F07F61A7DEB1" modified="2022-10-12" version="1">MKIRIILLLQIVSLTYCQQNDEILGFMYVFAQEWPGSICKFLNCTKTYMGNYDNARWNTHGLWPNTMLETTCGMIFNCKDEEYDEDKLTIATKTLIDVTWNGMYADTFTFRKHEWEKHGTCHPDNLTQNGYMSRVGNLNNQYNYYKILASAGIYPDDDRQLTDREVRAAFTKVLGISTAMTYTCQKDPTTGKFYIAELRTCFTQAMKPRTCDCSKPISAFVTCGKTFYYPTFLLSVNEQIEFEEIPDLSNSFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCC9</accession>
    <name>A0A8S1KCC9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190347</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCC9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="164" mass="19125" checksum="BA0F5D9B545BFDFF" modified="2022-10-12" version="1">MVQFGNKMNFFIIGGYLDNLQITNQCEAFDCINKRIIKLPNLRLAFLTILLLTKYVNQLINRIFIEKLLGILYYNILIVINGFNDVFNLNRIKLINLLGGYSENNKGSKQCIILEINDKTAKVIEIKQLEQYTNYSLGKDLGIIECSIRQLKQLFSRFQKNFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KCX2</accession>
    <name>A0A8S1KCX2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0190049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KCX2"/>
    <dbReference type="OMA" id="MQGLPQK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8052171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="93" mass="11050" checksum="B1DAA028630EDCF8" modified="2022-10-12" version="1">MQGLPQKKCQLLFKEQMPVKKKSSQYVKLKTLTLSNWKSKELTSQLLSPIKYQPPPIMEPRSQSRANFFQSQHIIIKIFDGKQNFIKKQYFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KEE8</accession>
    <name>A0A8S1KEE8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transketolase-like pyrimidine-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0130368</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000010">
        <property type="protein sequence ID" value="CAD8049254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="AYCKCIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="5">
        <location>
            <begin position="122"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8049254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="416" mass="48060" checksum="1BDA2AAC3B0124C3" modified="2022-10-12" version="1">MYDKIEKTPPVFIKFAEKLIAQGIVTKTEVDQLMKKHEDNLEVAYQKSRKMDYNLKDWQPVSWEMIKVPTLWGRIKDTGVPVTILKQIGEKINTIPSDFNAHPQIRKFYEERLNWIQKDQGVDFATAEALAFGTLLHEGFNVRLSGEDVQRATFSHRHAVIHDQKNPNGPSYVPLHKVIPKGQEDRLSIYNSHLSEYGVLGFEYGYSITNPNTLVLWEAQFGDFANGAQIIIDNYIASAESKWDVDSGLVMLLPNGMDGQGPEHSSGRVERFLQLSDDDPAVFERNLGVRLKRQMRNSNMQIVQCTTPANYFHSLRRQLRRDFRKPLIAMTSKRLLRLQAAKSKLNEFSEQARFSQIYDDPFPEQINEPNEIQRVILCSGQVYYDILKKREDLKVKNTAIVRIEQLAPFPYEFLXQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KFY5</accession>
    <name>A0A8S1KFY5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0200175</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KFY5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF98">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="15"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8053133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="654" mass="76771" checksum="140C5272FD53F255" modified="2022-10-12" version="1">MQQHKVIQGNTQILYTTTQPYFQGTKYKLFITSECNYVVKIYDVGQDKYLNTELQAVHLFKDNECSNIVKSYEGELQPNKKVIVFEKAEVLQNILKDSQEGFVEPQVLKILLDLSKALQHSHSLGITHRDIRPENILIGLEKQANYGILTLIKEEIEMNTFEHVRAPEQKDFSQRLPITTKVDIYAFGKLMYYMISKVRYDQNPNWEGNALWGVYSSKLQNLIKQLLIINPKDRISAEQIEQYINTIITQYNGISQNIHLRSQSTNEIQNREIITSNKELDKCHSFEFKDLQQPQQSSLSTRLVKLVSKVAQKTDFWVAACLEEVDAAPCQKYFRYLHFKAWQKKQIIPKFYEKVSNRLQLHSVIVTFKTLQLIHNYVNKGPQEAIAVKHSIYFPNAILERIKNFQEQNQMEISKDKFRSQFFTNFLYKYSIILLEKYFEGNYAMIPFFNSMNGIQKQKLSIAIFNNMMNLWKQLLNFTSNIQLQEQNLINLQLGLALTMADKIYNILCTFTHIFYALKQSTNYISEQPTNNNEVKQAFLKLQDDYWNNFQQTTSFFTVCKRIPQFQQLIPDPSKNLVEILKNVPLFQSKRGDFNFNDFLSYSMSIDGIKLSQSYGEIMINQVIEYDEEETVEIKPKYKKQNFNTIKAVISYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KGS2</accession>
    <name>A0A8S1KGS2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0200181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KGS2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="59" mass="7185" checksum="B1B6B8A9AEDF26FB" modified="2022-10-12" version="1">MMNNDVHFSRILIVALSTQNQDYQQNQKAMLFYSKLLEGMRLNFLKDYPNYYQLQKIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIG5</accession>
    <name>A0A8S1KIG5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0210051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021950">
        <property type="entry name" value="Spt20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1473"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1486"/>
            <end position="1495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8053775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1531" mass="184492" checksum="CB2360245E5BC8BC" modified="2022-10-12" version="1">MSQNQEKLESELFQQYQYAIDLLENGNETKGIQILKDLMQNQSIINQDDKKILYGIYMSLAEIQAQSDQVENKCEALYYYYQSTQISDNCWQTQRKMALIFRQLGMMPRALNVILKALQLCNQPNLIQAILYQVCSICFLMDDQKQFQIYFDKMNDNNNLKLKIAELNKYFINKNNITDFVNDLLQEQSQIQQQLEKIPFESSQKLKYFNQTFQFEIKLDTYDLKKFFKKLQNILQINVNFEPKKQDFIQDEKCFMSNTKIIVISKEQQQNSKDISKELPKENQPVKQSERQKSKKTKQQQDQQFVLDQIIQSNHNKLIELIKQFDGNEIFQLNNNNNKISSEQNEKQECPVAKFLLEQLNNQQFLTIIQLLQKLITLICQDYQQNDNFPQQQQDILNEQQKSPQNKNFYGSSYHEALRGLINCIVWAQYSTNKFTDDPKIKLRLLEVAYEELTFRFKEKKPKSPEQQNKIKNFIRFINNMKIELLQTNINQIINDQAEIQTYLSNYYRVMAYIHSDINFFQGRIAKLCAQELNNPEITQEILDKIKSAQFQISSNSPHQSQQIQQILTQWEQKEQRLEDNEKIRTQINYVQDYLQQNQSYDLIKRLAIICLNQFYFVQQKEELKVIYKFVQPLIQQFLNINQPDLLIGFIFLKFSIIYSLMELDSSEFLAKFIEIFLTQTNGYNKHLILMEILRFHHLNINKLFTYINIDLILQEFIKIKINMKNRYNQLIDQYKNNVQSDLELRYYLIHLIDLKDQYTPQFIVQQNELQIEVEKFETKYSQPESFSSASEDEVEQDEIFQKQYQNLQLTQDQIETVFKIGKSNYQLPTKIVEFLQNHTIKINMGDPKIIEHIKRFAKEKIRIEFGLSTKELSKNFLYIFLKSYYLQQEFVRDLSEIELQFMGYFLTHFPKLLQDQRQLNQFIIDIVKKSLQKFNDVKIQEYAEWLYSQIFTKQKSQISSIKEFQEFINQNNLQCDYKSNLYYQIQKSEELNQNENQQKIDFKNICYALAYKESPFLWNYLYQGTFEQAQKQFKQKTQTEIDWFLSQKKIQALVNKNPQLNHLNEFRYIMDLIKLKQRQVEYQHSNNEQLYKKTEQGYQALYQKQFNVPKDAFEIENNFRVLQLYEKKVMRKYRNIPIERISQNLDNICSLVNLIKQMKEYEIEYFYQSNDGEHEMKQDLYINIYKFIRRFLRHRLYNQLNQLVLIIKDKFSYLLNQNQSDLQFQFQYDNRKFKISNFTQEQLEQEENTEYNKQDVNLILNNLLTIFIENTQKLVRKKAQRYVIESFYYATHLKFNSQASIDQVFAQISSIYQPNSRDLVYYYLQIDKYKCEKDEVLSQYYSQDLIFNYQKSKIIKLIIKILLKMQKYKDIYQLFEKLHKTYDLRLYQACIGLLEEYKRIDDKILIRDIILKSESYRRTIDLFKQDSIVPLLNNMYIRLYEKDQQEQNEGDYVNETQETKLEKGKKIIEDYRATKRKPAKPKDPQQQQTQNSQAQIQTQQAQLIQQVVDDVGQYINQGAIIQSFNDFDNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KIJ8</accession>
    <name>A0A8S1KIJ8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0210082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KIJ8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="398"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="529"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8053805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="602" mass="72514" checksum="3EC8ABBE9593C6ED" modified="2022-10-12" version="1">MYRIGDQMSKTSQSRENSNSNLEECMHNIQSNLKMMYGKDEEEVHLGSYNVNAVSGFHSQFLSNKDDQYDSQRMHYTSSQSNDFMLEDKNKQIRQLNKELADLRKGQQMTQFEQYEHYQKIIDSLKKENVLLQQRLLNNEAVQQLNSERVIEQQEQIKEYEQKLDQQNQQISSLNLQLKDTKDQLKLYTTKSQISKQEQQSQSKHYTQLQQENDELQRRYIKQQLENENMKVLIEQLQNDIDKRLETQNDSQVVEIIEQVLQTISQFVREYDYELQKSEYQYKFSNELKEIIKGFITQRDLSIWQNIDRLSSLIISLFEQLTFDVKQIHSDNIKIKNMTLQIESLEQMTVQLEQTISQYEIISSQQQNYLRFNDDQNKQEKEELVNVLINRTLNDQQINCYDKMIKNLEEVNQAQQNEIKELKDKLSIQIEQNYLYQKQYQEEQHQQKIYESKFKKQHYDSLFLKEIMNKLVILIGNEMTTKTIEQIQELQQKTNDQYCDEIDIKSKLEMKEQDLKLQINENKLNMNDMIEKRREIEVLRNTLRNCQKQIEINKEKIDMLVRDLDHNVMQEKSQQFAISYLPKTFKKENENLAQKLQKTMRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJP2</accession>
    <name>A0A8S1KJP2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0200267</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000017">
        <property type="protein sequence ID" value="CAD8053316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJP2"/>
    <dbReference type="OMA" id="YQMQTIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004128">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051872">
        <property type="entry name" value="Cytochrome_b5/Flavoprotein_Rdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237:SF1">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="5">
        <location>
            <begin position="178"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8053316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="255" mass="30662" checksum="36E0A6AF9715C13B" modified="2022-10-12" version="1">MNINNQINFYFANRQYTLIWQDLDPKIINEVIFFVFNEISSKNKLNNLIFTDSNTQPIQNSLPHFIQSQHIFINTLNSNNTSLINKEQEWEFQNLYQIHQNQLIIQKYKKMQKMETVNYQMQTIFNLNQQYRETSQNIKDNTNMINQKQSIERSASTYPITKIQSTNNQIIHKRQAELKSYTIEEVQLHNTYKDAWIVLQDNIYDISYYIDKHPGGREQILRGVGRDATFLFLQHHPWINFHYILEKFQVGYLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJV2</accession>
    <name>A0A8S1KJV2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0220359</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8055017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJV2"/>
    <dbReference type="OMA" id="QQELNFC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="19"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="344"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="466"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="538"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="646"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="715"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1019"/>
            <end position="1076"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8055017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1111" mass="134806" checksum="F37AA6BDBC3754F4" modified="2022-10-12" version="1">MNAASQIQNECVFIEGKLYENLKQFDCQGVQSITFIGNQTRNENENFIIKLYKEISDEQIEVLQQIKKYQNQKIPKCQHMIKIIGIQNFLNIKKDRKFYVVMEKGKDNTQNLIKSNISFSLFQKFKIFEQMVRGEMELHSLGYFQINLKPQSFVYFETQTQNYQVKLIDFGSIKHDSTYFKTKQIETFNYLAPEVIQGNQLYNKTVNIWSLGIILYELLVSEDFFLAKNKQEMKEQMQDLTQDWINKNINKANCLGQTEKDLINSMLQIESKKRIQLEILLQKIDQYLTNIQEYQKRIVMQLSVIKTTNQQFIQNLMEKLNKINQIETAEQKKKQLVICINKEIKKYQEILNENEQRLQQIQQAKMKDEFIKLEQEIFQVNQQQQLQEYNLIMEKITKELRLVEQLLKNKEIENQQKLKKEKEQFINFLEIIRNQFRLQSNQIQTLSEQYNLNQRDQKQINEQEKYLNVRKQHQKMEEEIKELEQSEVRIMVQCDEQKITIYQQFNTKLKEIQQAQNVLKILIEEASKSILSFNAKIFEENSNQIDNLNDKLTDYLDKFQYQSKNQKYSNKIVQIMNQIKFVLEQLNSLKKQINQCTQTSYQQFNKNYYEIEELLRYYQKQYNQLHEQIYNDELIEQINLERIKKIKMTQNQLDQIKKKMNQLKDQIKYLFHNKYLNYEQIYNLINNKLIKIDQNIIKCQKKFENFIELNLLNSYEIMKNQIKLLQEVQRKLDRDVNEIEYNIDQFKLIISKIETENESEEKKEIFNLNTQLQETYRKFSKALNSIHFQFESIELYKQMKVYQDNFQVELKQEIQTLKEYLEVQNINTKLFQIEMKMRDIKNNKQQQLIVELLQKLQQTNQQKGYRLKNVFNRVCQKQQELNFCFMDNNLIKNYEYLKMQESQLSIELNLINQKTYDRQLNSQELIILKEQILQVQSNIFDLIMKIPKKNKIQEFNKAYQQNLESYEKLLALVNYIKQYHLTRYYERLKENKFKKQNKQQIQKESNFDKSFQSLLNDDQEKYAINEKEAQDKLQKYENIVNNIYNMMTKEIMEKDKQQMEKQLIEIESQIKQQNLAILESNFNDPSVIENIIYNRKIYKLNEFAQTLILYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KW68</accession>
    <name>A0A8S1KW68_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0250134</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KW68"/>
    <dbReference type="OMA" id="TFHPYQA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039647">
        <property type="entry name" value="EF_hand_pair_protein_CML-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10891">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="468"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8056946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="496" mass="58527" checksum="AD9FDB2BAB77810B" modified="2022-10-12" version="1">MELLDFLMECAKIERTIEISRQFLIKFPTFHPYQAFKQIDQLNDGYITAQTIQVFLHRFSYLHTLKECGILFYKPEFKYVDFLRFLLPTNQLLRDYISHSDQQSQQSNECLKEIAKHISLEIVLMNNLQPPQSLDQLTDGTREDIEQLFKKERLFVYQDEIDAMIKRLDFYGDGQIDLLLLNNWVLILNKQTQIKQQKESCSAFVTPKKQIQFQNSIKTTEKKESKNNTTKLIKQSPQKRNQIQMQKSKKQVMACQKYKQDNRTKMTQNKQEVETEKKKIIEVQKNKKNINTQTKNKSKQPQEIIEYLLDKEIKIEKIKQQLALQKDFNTLDAFRALDVYSKGSIGIEEIDTLLNQKSGQSMQIFKRFNNNQLTYHDFLYLLQPYSANFAEILNTRQPSVDGNNKKMSELFSYQTIKLLIDLLTLLQQIPVKLNADIQLFNKIDGITRDNFIGVSDLQQYLYESGLRYTHHEAILLINAYDKDKDGRLSLQEFLDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXN9</accession>
    <name>A0A8S1KXN9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0290289</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXN9"/>
    <dbReference type="OMA" id="YNIYIFT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000121">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="215"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="401" mass="47313" checksum="C7123CC3EA4B8786" modified="2022-10-12" version="1">MDSQTNLRPKIFIQIQNDFKKQFYSDDDADVTPKRPLITKPSKFSKGEGQQFQTDDSDNTPQKQISPSSPKGQNKLSKFKQKESHIEKDSDIQFDSNEEDKKEQPQNIQIHQKTDPEYDIQQMSRQMEQKYQNPKPAFQHPPIQKSNSSKQVMTKHLNIKEHPFGHLVYGKNINQSDYLRFLQIVQSGLLYAHHSLKGPSDKFLRSRFIRLRESIQRKPKFLILDLDETLIHSCTFRDSPQFTITLEDEDEKVDLCFNVRPFCKEFLQEMSNYYNIYIFTASSELYANAIVNHLDPNRQYINDILCRNNCFETKNGFFIKDLRIITNRHLKDIVIVDNLPHSFGLQLENGIPILEYLQNPKDEELKYLQKYLIKLSKEEDVRLFNKQNLKLLSLIDFKLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYA0</accession>
    <name>A0A8S1KYA0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0300160</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial ribosome (mitoribosome) assembly factor. Binds at the interface of the head and body domains of the mitochondrial small ribosomal subunit (mt-SSU), occluding the mRNA channel and preventing compaction of the head domain towards the body. Probable inactive methyltransferase: retains the characteristic folding and ability to bind S-adenosyl-L-methionine, but it probably lost its methyltransferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052571">
        <property type="entry name" value="Mt_RNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015324">
        <property type="entry name" value="Ribosomal_Rsm22-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184:SF5">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09243">
        <property type="entry name" value="Rsm22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8060489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="994" mass="114784" checksum="EA11780A831EA480" modified="2022-10-12" version="1">MIIFRASFAFARRIKQLRTSSINNSKIKDDENISDESIESKKKEEQVDITQNQENLENNQKKVFEKKFIKSQEFLMACSHQPALSLPEEILKKARKVFSKYPPDELREASYKYMRMYQLLHALEKPVSITIKRNNLFENPENLQKLTEKKVIHLYKQGTSEQQILEKIFERRQKKDINVDRINSAEEKLEEFHNFASAIDYDQILTVSYFLRKVPGSFAVAQKIMTEIQKRNPNFNPKSMLDYGSGIGTWAWSFKKLYPDVKNIVCVEPNLYMRKLGKFVTQDYIKDIKFYESLSHTLELSFDYFDFISIAFVLEEINHPEARLLALQSLWAKLSDDGIMVLACPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVSITIKRNNLFENPENLQKLTEKKVIHLYKQGTSEQQILEKIFERRQKKDINVDRINSAEEKLEEFHNFASAIDYDQILTVSYFLRKVPGSFAVAQKIMTEIQKRNPNFNPKSMLDYGSGIGTWAWSFKKLYPDVKNIVCVEPNLYMRKLGKFVTQDYIKDIKFYESLSHTLELSYDYFDFISIAFVLEEINHPEARLLALQSLWAKLSDDGIMVLACPGSPTGFRFVNDFREWILKQKGFIVAPCQHQQSCPMAKEGFQWCHFKQMFTAWPKDVFPKYIYENTAVTEKFSYLAVSKKPLIQEGQEWPRITFDPLKRGKHLILDMCTEQGQLERRIVAKSHGKEGGYYEAKHLNGGDLWRFPKLPPSKVGKKKRKQSVKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L217</accession>
    <name>A0A8S1L217_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Eukaryotic translation initiation factor 4E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0300012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L217"/>
    <dbReference type="OMA" id="MWEDDVN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000044">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF18">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E HOMOLOGOUS PROTEIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8060195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="19718" checksum="92EEB4335FFB20D9" modified="2022-10-12" version="1">MLLKSKYTFWVSTQRTQTKIDKDNYNDNLYQVCKFETAEQFWMYYQWIAKPSTIPEGCQILLFQEEIQPMWEDKANQNGGRFVFRCKNNVDRVWENILLSYIGNQCTYNEFVCGVVVNQKRSFTQVSIWVKNMTSYQTQEDKIIQWVKEMFGINELEFVQHPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L2L8</accession>
    <name>A0A8S1L2L8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0280096</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8058916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L2L8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8058916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="34691" checksum="533D84F10A11992B" modified="2022-10-12" version="1">MGQECQKCQKRQEVHSNEINKQEVISSDSDPEYEEDDEKQPQKPPSMDSKSNSSTFIKEQYLSSDSKQEQIESSQNQLNFQDGGIYTGIVINNMANGLGRLVMPNGDIYEGSFINNKLEGDGKCIYARGPIYTGQFKQGKSNGFGKEVWPDGSVYEGEFRNGKKNGKGIYKWSQQSTYNGEWVDNMINGVGKYEWPDGRSYYGQWARNQMHGRGYYRWIDGKYYDGEYENDKKSGFGIFNWPDGKQYQGYWFDGKQHGKGLMINKEGKKKFGEWQNGKLISCSDESNLQLIPDGWFLNTFNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3N6</accession>
    <name>A0A8S1L3N6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0300022</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L3N6"/>
    <dbReference type="OMA" id="RMIQEPQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23804">
        <property type="entry name" value="UBCc_UBE2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000031">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="50"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="205"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="226"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8060213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="239" mass="27925" checksum="A6421659EE847983" modified="2022-10-12" version="1">MNEIFIHFYVAFIQFQAYLLTIQRIQFKITFIKLTYQVIRMIQEPQINPQLGPIIAKQMQIMIKQPIDGVHVIFNEQDVFDIQADIEGPVDTPFQGGVFRCKLILPPQFPQVAPKGLFNTKIFHPNVSEKGEICVNTLKKDWNPLQWSLKNIFEVIKCLLIVPFPESSLNEEAGKFFMENYDEYFKRAKLLTSIYAIKQDGNVLKGNNQNTNDEADKKNKLTQTQQKKENDQKKWLKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4Q5</accession>
    <name>A0A8S1L4Q5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0310197</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4Q5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8061215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="57" mass="6500" checksum="ADC25EA8FE8148DF" modified="2022-10-12" version="1">MKSKPNLSITVNGAKLLQDLDKLKGKSKQDPYTLIQLVPQKVQNKISNKLMKIFNME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L955</accession>
    <name>A0A8S1L955_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0330009</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000032">
        <property type="protein sequence ID" value="CAD8062183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L955"/>
    <dbReference type="OMA" id="NMENCDS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="7">
        <location>
            <begin position="80"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="206"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8062183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="30121" checksum="9CB2FED5514B2662" modified="2022-10-12" version="1">MNVVNWTNNNNESQSKSIRQSSETLPVIKKRISKRLQKHIQDTSFKELYKQQKLAEIVQKAMERNECQYEEYDEIKICGEIIKLNDKVIIKNEDSNVEDYIGAINKICSVVELSTLKLICLCEVQWFMRKNEIICHKPRARSWIGNQEIFSTNTNDYVLAQTIVQKCTVVDCEEYFNMENCDSTTYYNRLEWDVESKKFTNMNTIKLYCLCQQPWNPELNYIQCDKCQKWYHFECVGLKEGSYENKEYACGYCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCP4</accession>
    <name>A0A8S1LCP4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Aurora kinase</fullName>
            <ecNumber evidence="15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPRIM_AZ9-3.1.T0350309</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="15"/>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8064125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCP4"/>
    <dbReference type="OMA" id="RRTQHTV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001241">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="17">
        <location>
            <begin position="5"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="56"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="130"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="179"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="11">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8064125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="404" mass="47239" checksum="2E5310AAA46D0724" modified="2022-10-12" version="1">MMSKCKSCNDEGFLTCPLCQKTTYCSKKCRDYDWAVSHKLECKAELPKRNVQDFEIVNQGSLGRGSFGCVKLARDRSTGLLYAMKIIEKSDVSIENLRREIRIQKKLQHPHVIQLFEFFEDEQYVYLILEYAENGSLFGYLRKRKILPENEAFVYFFQTCLGIDYLHKKQIIHRDLKPENLLLDKDGNIKICDFGWSAEMMITQTRNTFCGTIDYMTPEMLEQKPHDQTLDMWCLGVLLYELTHGQAPFKGRNDFEKCQNILKQEQFEIKASDQAKDLILGLMKRDSKDRLTMDQVFNHPWMLTMAKGYQLEIKEYIFEEKKNLDQLSSSLRLDTKFSNTNTTTTRLSGSNNKQECVSLAFSMYSDELQPPIQTRITRRTQHTVKKESGFFETIFQNLGCTKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LCU2</accession>
    <name>A0A8S1LCU2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0370236</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LCU2"/>
    <dbReference type="OMA" id="MIIFINT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="383"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="757"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8065367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="887" mass="102327" checksum="51B4D9DF8AD97F4D" modified="2022-10-12" version="1">MNGNQFKNPEIEIIAADEKQSLQTQQQHISICFCILLIIIYSVLLGIEQRQLPLMICSISYNTIITMIIFINTSKINKKWYLTMIIFSKLISLVVLLFLDQEHLIIIAILIDSINHESQPMRDVEQTIIKLSSLIVVCINGNLIFIMVMISILLLQLVKDLHYKKSQMKKQLNATNITPNQNISQYSQIEKQNDEIWKNRMQTIPIIVIIISMQNWQIVHKNQFFYDFFSLYNKTEKELENLVMYHLSFHVSSNNLEEQDVSSINQFINQIRRPHTMTFNNNFNLRNQETLQDIPVGYQSLNEIVLNFKSKIQSSFLNIHHSVYYEISCRQQLEDGSYLQLFGQIVQNEKDNELLIFLNDVSGQNEMQQNIQLNDFKSKILQSFSHELRTPLNSALNFLSSSLQEQKVPLHVKDNLIEPAINSLKIQQYLINDIIDFSSFYQDRIKIKLREFTISELITEISSMFYYQFNTKQLAFHVDLKENQLGSFCTDYKKLLQILVNLIQNSLKYSFKGGCVLKLISLPNQNIQFEIVDQGIGIQQNLLLKLQDIRKNVNKNKEFVKDWHGIGLLISSIILQYLAPPDMTFFDIKSEGENKGTLISFCIKNFFRVPASQQNIKNSTQNCMNIGSASSQFKQLSGSQQLQVNSSLFNVMGTVVKATEFHITSTQIKTQFAQYPSKKSQNDSFYSESYDDYLEKRLEDLESLNPFLITDINNVYNKLKDKSSKVINLKQIKQLEEKESELFITGLNSLKKCNCMRILSVDDEIFNQKSLQILISKMGFEVVLAFNGQQAIQVVLGLQKCCSSCNLLSLILMDYQMPIMNGIEATKQLISMMNKSQIPQIAIIGLTAFTGQKDIDNCLKAGMQEVLAKPLNIQELKHILLCVFKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LDI3</accession>
    <name>A0A8S1LDI3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0360115</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LDI3"/>
    <dbReference type="OMA" id="DVVIWIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051001">
        <property type="entry name" value="Calbindin_Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972">
        <property type="entry name" value="CALBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972:SF10">
        <property type="entry name" value="CALBINDIN-32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="27"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="124"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="167"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8064415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="208" mass="23948" checksum="DCE9498B1017646F" modified="2022-10-12" version="1">MDSNKLRNSGYQLSDRVQFGGIFEPEKCKQIAKQVMELYDSNKDGYIEQNEIAMMLSDAYRAMNKGFNPNSQDVSNCQAILDRKSTGRVHNEDIEQLVQKYFSGQPPAQPVKRVPEKLSRIAQERLEVARRIFKFVDKDNSGVLTEEEVPELLKETYKHMGMNDYEPTKEDVVIWIQMTDTDGDGKVTLEDYEQLVLDSLRKQGISLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LE18</accession>
    <name>A0A8S1LE18_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0350143</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LE18"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035894518" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="223"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8063793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="40736" checksum="6C1E654E8B586F74" modified="2022-10-12" version="1">MMKFIALALIATTVLAVQKDTKTILAEIDADNFGNSILSTVQMYLQAKGNSEEILMLLNQLLAGLVDDQNKHDNVIRVDRSACTRIVTDLENSIAYHTAQVSANAQMREDNEKALAEAETDVRQTIQDIESNERTFAQEEANRNKAHETWVRKNGEHDDAIAAVDEATKLVQHLSLGATFAELKPKFEAVQKRLIENESHGALFQPIVTALTELASKVDQKAIQRILQLLSQLRQQLVEARSVLEDTENRQAQRWVEFSTHLSNEHNRLVDRKNQLEQAIQTFKTNIDTATHFFEVHTLEVESAQETLDAENEWCALQEQTYETQSTERQRQQEIVERILEHLTEKLTATSQYLGGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFJ0</accession>
    <name>A0A8S1LFJ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0340363</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFJ0"/>
    <dbReference type="OMA" id="CYNDNII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="136" mass="15955" checksum="AC0DCCC52CC11AAF" modified="2022-10-12" version="1">MLSKPNNQDSNYSTVEIKQGIKRSVKEISNYAEIKASKEQFKATKIKQKSYIRLKQQNRVYAFPCYNDNIITHQKGENQLLRPRNQDDDNESDDEIIFQSLRFSLNTLVEALQNENKRNYSFAQFISNDLLNSLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LFU8</accession>
    <name>A0A8S1LFU8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0390155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LFU8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8066567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="411" mass="49038" checksum="2466E3CC5D55115E" modified="2022-10-12" version="1">MKKWKEKLLIFYLNTQVLQIQDVKDQTQKQIRIYDYTFKRLGRNQDKYVIKLQCWNNEKEYLQWCNCFSGEWQKKLIQRKVLEEFDRSLPEVEPYIDKQPLVIKSQAKPSLLSREKIQKMIPYLPDFIQKDMEIFFQNTLQNLDSFIDIQNFKQILDSLNVQLYQDTNDIIIKLIVILLNIQINIKQLDLLYKQDYKSLLIEEIYVECQDKDAGEKMLKVLLMNITSNDRNLIQIQEQIQQITENVITKNYENLDTLQISIKLTDQEIFRITSEQHQIKSPEEFLKLIDKVYTGEYINEAINSYSDQRILQGLTLQLDSTEEDGHFLYTKHYRVDSQRGGFTYHNERLLRDQRSVLLNMIKRIGSNIINGKSVMSVSLPIQIFESRSFLERMARAQRHAPLFLELQPNQLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LH87</accession>
    <name>A0A8S1LH87_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TRAF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0400145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LH87"/>
    <dbReference type="OMA" id="CDFRICK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="159"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="51199" checksum="67C565A5AA07F747" modified="2022-10-12" version="1">MQFYDRLHYIPIGIPNENLLCKICKNLAIDPVECIQCENLYCKQCFQISKSSYDKCPEENCNGPFQTKQPHRMIRDQFSKILFKCINHNEGCKIEMVQENVIKHMSECLYHQINCKCGQILQRRNIESHKNMCTFYKTQNCLICQQQLTLEDLKSHRCLLQLQQMVNQFQEKFQDYKEQSNFAILEMKNQQNQRINEINSSKQQIITLLEENKQLKNDLKTRQQIYENNQEKINQKKAQMENQQKQQQQQQQQQQQQQQQQQQQQQQQQQYQQQQQQQQQQQQQQQQQQQQQTQLITKGELIDCNQTFCEKNHKLKFWKKPSGEEKKQKCSKCQKQNTTCRYFCQQCTIFVCFKCCFPELKIEKQPNQPQCPSKHYLNQITDDFRCSVCDKKGDDMIQPIALQCTYCDFRICKQCIKNKKYQEMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LLM5</accession>
    <name>A0A8S1LLM5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0420264</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LLM5"/>
    <dbReference type="OMA" id="KECFIAQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="2">
        <location>
            <begin position="558"/>
            <end position="893"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8068647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="893" mass="105693" checksum="2633644C3C36F8BE" modified="2022-10-12" version="1">MSYLSQSSRLTFLLIQLKSKALITPDQYSRLCDHAAKDHSQLKELLVSYENGLSQSDLIDELSKMLNNQNGNENIVKSYFDSIVYICKQLKNKIKEFYRDIKQDINEIYKECFIAQHHQQEVKDLIRLILKQFYIKEFSLLQFKQDHQCHILTNNFESIIIKDAQEIQEFTQQQSHNQIIETPLDYLSLLKIEKLPQYYIISNNDVFFLTFTEKVNNKFKQYLMITSLYNSIISIIKQGNQIVGLKKLQTIRMEILQLVSRTINILTYKLIFHILIQIMKQFNLQNLVSLIDKEYLMQQKVTDYLLLTFNKEWDSIYIDLDGNLSIQLKEKIQSTFRKSIKRYSKKLKQKKNLLESFQQVTQHNEMIMCLFFNSYLYLYYMTVQWNHDQFVSRFKLSKDINYGDPIYKIFEKNPIVISKVKELIESKNDHLEFDDGFYKFSLKVERGIKQDIKQILLYLFNIQLKRPLNELLQIFRNKVKILLTLKKATLAFKSKQLMMENQFFRQSALVMYLPDHDMRKLAIVYNQDHDQALEKYRATTMKKKSRKLASFLNKYEEEDKKNPDGRKIAIDPNLQERLLDYEFNLLNKKLYKNKFIIVYNIFDMLEYTKQYPLKNKEFVNFLTALKYKYNKTANPFHNFTHGVNVMHGCFLFAHHPKFGFCFNDQQRFAMTLTGLCHDVGHPGTNNLFQINTQTKLALIYNDKSVLENHHIAVTYKLLALKQCDFLESVPRADKIMIRKYVVNNVLATDNQYHFKLLNDIEIKFGQSQGDPKIFQSEDNKILLSGFLTHAADFFGAAKSYQVARAWSERLRREFQAQSQLEDIVGIAQTPYLRNLDDEVQYSKNEIGFLKVIVKPIYESLNQFSDGAMSLQLANINLSIKKYSEIVDSHQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LT12</accession>
    <name>A0A8S1LT12_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0420297</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LT12"/>
    <dbReference type="OMA" id="TNEPLEY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025:SF3">
        <property type="entry name" value="HORMONE-SENSITIVE LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="4">
        <location>
            <begin position="430"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8068713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="672" mass="78063" checksum="441EC339CFC94488" modified="2022-10-12" version="1">MELLPVDFEQLDQEILAALFPIQQQPLLCEEDNLLGHVDLLFEQIMTVDNHQQLTIDEFKLNCTLIRNTLQDLIQLMKNLIHKYIKSICTINRIQYAVNYLIILKDLYETVLELFANSLEQPSPRFQELLTKYSNNEEFRIKAKNIQDINFFGLQHMNHVFTQDTLKQIIRISHHYLTNHDSQALEQKLIVYNSHLNILVRIFASAIDMNKQDIICLNKNHLFWRQIKAISTYTKLSQNSEQISQSYYNFMKSIRIGHAIFLKKSKVKNSLMRDIIFISSSVWYFVLDGRAKLRAMEHMCDMQVESAMSVMGMVEKPGIKHLIEFGITSINNNIKIYIDPVVSPLTLKYMNKQFQKGILNKITNEPLEYVNKSIHYDIKQHKSIMTKDKTKLRIRILSSKSLVKTNSFQQSFFSQIQHEALNYDTFETIIIHFHGGGFISMSSSSHQNYTREWANQLGIPIFSVDYSLAPKYRYPQAVDDCWQAYHWILNHLQYHFNIKPKKIILAGDSAGGNLCCALTGLAIKFGIKVPDGLLLSYPVLDLKMKYSPSHMHGLEDFLLNHTLMDICIDAYTNHPASYEFDPFRSPNHFSDEIISKFPPVRILVGSKDPLIDHSHRLAHTLIKNKRNVKIIVYEGMSHGFLSFYMLGGMKESSKCIEDSIIFLKELISQKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LT38</accession>
    <name>A0A8S1LT38_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Defective in cullin neddylation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0450123</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000045">
        <property type="protein sequence ID" value="CAD8070229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LT38"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032182">
        <property type="term" value="F:ubiquitin-like protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014764">
        <property type="entry name" value="DCN-prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005176">
        <property type="entry name" value="PONY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281:SF31">
        <property type="entry name" value="DCN1-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281">
        <property type="entry name" value="RP42 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03556">
        <property type="entry name" value="Cullin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51229">
        <property type="entry name" value="DCUN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DCUN1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU410713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8070229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="164" mass="19611" checksum="89B0D6822EFE5F70" modified="2022-10-12" version="1">MRDVGIQDDQILQLYITYKFGGQKGAVFTLDEFLLGMIRLKCQSIKDLKNIVPQLKQKIQKENKYKKLYSYFFKVMSQGKNVIRFTEAITLWDSLLNEQFKEIIDFIQFCKAKPAEFQNTTKVSFDLWCQVWKFFEIIGSDYTKFDENDAWPLLIYEYVQFKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZ41</accession>
    <name>A0A8S1LZ41_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0410214</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZ41"/>
    <dbReference type="OMA" id="ICKTKSH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="560"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="749"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="957"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="769"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="957"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="967"/>
            <end position="987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8067884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="987" mass="117183" checksum="6519578E3F206FA4" modified="2022-10-12" version="1">MKPQPKISEHRNFQVNQQQINVDDPQDKTGIEIFSHETQLRKREKLSPILRSNSGHQEQLIQQYNNPQQLITPKNETITERLQTFGHVDLLKQSKSFRKLIVYFHQKDFIKMLLNPFKLTSNFTLRHFNLINDLSASYKFQLGRSQETQKSLIHQYISLIDIRIQLRLKFRTIKTKCKQYLTHIHNQIPLIEPMSNAKFVWDLICFAIRIYLIVVIPVIIVFHGQNFVDKQLVPLLLFSIALIFDIVIRAFTITYDQGLPVRDRYLLFKKQLNFSTALEFFSFIYTIIITIEDDKIIDKYILGDGWPKFLLALLYIQVINILKFIDVSQYSLKLSRMQTSIVDLIKLITMILLVQHIFSCVWIVFGVYSLQNQEDSWLNKYDNDDWSFQFLQSFYFICVTTFTVGYGDLTPKNPPEQIFTIIYMFLCMLLFSYTVNTIGSILTQIKESSEKIKSKLTAINQYMHNKSISPTLQFKVREQLYFYLKQEIIQQVNEQSEIIQLLPEDLQHSLKIEAAKSLLNKCSFFSDNFSKDILNQLLEEVNFQMYQPGAIIHSKEEFYIHIIEQGQVDVMYKKKIIQTLEKYDYFGLEEFISQQANPNITFKSTAFTSVLSIPYSYFHKILSQNDLENQKFHNLLTSSSYLQNFCFICKTKSHKTQLCPMVHFIPNKEVVIKKYLYRKKQKKRVSQERKLRLVFVNQIDDNEDNKLKQIKFINTKTNQKMVENAVIKFKNENQFYLEQLFPNIEQPISVDSQSESENENPDELKDQSFSEQNFNSRRASKQIPNLSSKNLIKYQVLEKMKKERLSKHIQQQQQSPLLMVSQYGQINPSTQIISKYNLKKIIQAKSLEDEQLQLIEEINFFDELRTKMNNLLLFPNKEQEQIEFWYKKLSNLKEDLDDMANFEQLKNYEIFNRQWNVDLVVKKTSLKPRKFRGLKKLKRYLLFPYLFINKYIDRKDTNDPNKTLEKSKRKRPSKPKAIKKTRVTPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZ43</accession>
    <name>A0A8S1LZ43_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0460165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8070841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZ43"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8070841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="148" mass="17686" checksum="CA5825410675B1C8" modified="2022-10-12" version="1">MNSLDRLKQQFQKTTSSLSNFHKTAANLQRQAYFQGQYDILAQILSYPSMDGLIEWLRKKSDEVLQILEKNSVRYQISKHPLLTSEPAALKMKTTFLKEPQPQYIQQQPQMQMNQKSFEEEDGNTYYKQNNNQYDYRERRQDQEFGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4Y8</accession>
    <name>A0A8S1M4Y8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0510240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4Y8"/>
    <dbReference type="OMA" id="LTITWSW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035720094" evidence="1">
        <location>
            <begin position="18"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="428" mass="46984" checksum="2BFA5F248ADAADCF" modified="2022-10-12" version="1">MILSILLIVNLITSSAAQTYVGETPCTGLSSGSCTASAISGIGYCYWESTCQTLPCYMIDEVAACRAGSGLITGGASTLCDSLETFSLQYDNVCCEKTEGKLNYAFVRFVKTTDGYFDKASDGTTTLTSLTSQTSANIFLLYTVNIWKIMPSFTTLPTVANFQSQLGAILNQYKTLATALHDLPDSHPFYLERTVYQSLQLLRDFIVLQTTSQNTMRDNILQQIWSIALIALFRMTKFQPNYYQTNYYFINFAIIPYSRNSIIINGQQHSTKIDWSSYAYGSNGYLMVYSFPPEMFGIKNALSDVICLRPIVGSSPVYNAAENTFDTGFKPLTITWSWTDTSLTVVAANLKLYKFPINNALESGITQEAGVTFTCNLGGKYCVSTSITATPANTGVNYFISAGLGVAADKNKVQCRLQGRTWSGSSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M549</accession>
    <name>A0A8S1M549_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0500102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M549"/>
    <dbReference type="OMA" id="ANDGQCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="106"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="401" mass="46221" checksum="BAEEEA8632C20349" modified="2022-10-12" version="1">MSLFSCSALFLTKDSQFQEVIITTHYGSLQIATKYGNEQLVYLPQNSMDTILCFARKIDEIKQMGTLDLNNPETQINVTFLLSMKNIEQLSLRLKGRQIFQASSNYIVLEKLAPGVKLISTLQRISVEKKAKFLSQDPLSSSRLGQLKQEADILRLLNNNSHKNVIQLEEILTDYKSVSIILEYCRGGDLLKLLYKKSIQIDVPLLMFNLLSGLKHLHDLDIVHRDIKLQNILFKDCQKMDTLKISDFGFACKKSEILYINPRCGTPGYTAPEVFSQPCKYDEKVDIYSAGIVFYNILTFKNPFGNLENISDLIKLNINGDYNETHLENTKLNNPLAYDLLTQMLQKESNNRISAYECLNHPYFKSHQKGDFIKEMIQIKRKFKKQKAINKRTKKEMVTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M5H7</accession>
    <name>A0A8S1M5H7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0500245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8073173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M5H7"/>
    <dbReference type="OMA" id="PNSLICQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="719" mass="84323" checksum="D956EABAFDF796E7" modified="2022-10-12" version="1">MKQITDNDNYSVDGWNFDEQSNFIKYTKFVIPFTILINIYLIVITFLYKTIYPPYFYEILLYSSIYGISLSIFNIFWKQEKDSVIRQLSFVLNGLMIIMAISLIIFFIASFEADYNYKIGENSSYINQIQEWQHFTLLFRSILLSLFLLLISYFYITYSLYLCQDQQNLMIALHIYNTLFFITGIAINHYSKLIGFQLILLLLLISTSTLVYSANLKKIKVAYFTIGAILLFYLILNQYQHFLNVRQAKQNKISVLNDCSAHLRNLHMDFLFANTICQKYQENVQCNDDQKAFMWELDTKIPISQRDTKLGCLNLNCCGIVADRSKFKLNVISLVHQLIGFATLTLILYISQKPPLLNTEIKIYDFIFGTIQLFLIIITIFLFSKEINQDINKFNSLQTSKYIDIPKGCSQMKNFITLNCQFIYCDNIELTIKTDYPMNYYRLSNSKDFYVDDSNFTELKFIGKFDAIQNYIYSNIYMCVTSKTVNIDFTIKKSTRLLKEISQTNLIQNQDILKTLQAVRFWKLQCQLNINQNYTIKLLNPITNTEYLQIQNQNTLNFVQEQSYILIIQSKGYIPYEQNIFINKDTLLNINLIKQQKQLSIILQGSKQSMLGTTFATSIYKQYTTTKWVGSNNYMHLEYIDDTTQIIKIDEVDYPILVFIINPNYDALLSIYDEDRLLYKSYTNKQINTQQVLCLQGNTINYLKSQTSNKLLPNSLICQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M7U1</accession>
    <name>A0A8S1M7U1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0470084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M7U1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="299" mass="35854" checksum="D34E4FF94D084B2D" modified="2022-10-12" version="1">MEDYSSSKNLLLIVGQILAESDNFEKFKKLIEKKVSNIKIKLIYKTKEQEGEISALKFISEGNQVRVLKIQIEQLDSNSNNFHVYQKERKPNKTRYSQLLSLSDPDQLNEITINLKEIIQFLEFETSMHRHQKIFWAWLESTFDEDKKDVRDHADYGFPDETIIFSLGQQMIKLIEQRKYHNCLKRIQILNRNYNNCNYKSQNSSLKQYQINVFLKTFKQQVLSEKDLIYCQANDFIKYAIKHNFAQFSNHNESNEFDQKIIYQQEHFVTRIEQLNQYLEVEICRIKVHLIELVFYPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8T1</accession>
    <name>A0A8S1M8T1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0520157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000052">
        <property type="protein sequence ID" value="CAD8074251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8T1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35316">
        <property type="entry name" value="28S RIBOSOMAL S34 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35316:SF1">
        <property type="entry name" value="28S RIBOSOMAL S34 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8074251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="141" mass="16417" checksum="0A151213E91C1F83" modified="2022-10-12" version="1">MYGLLKTAAEEMLSKAIRTYTFNDLIVIGRHPYKNLPEMLAQYPNNGVGFKVWRKTWPENKYIIITEAHFKGLRNGKFFGIEYYNGKPLTPQPIKIRNCSKRGTWKYDTNNTSGISANGVYFSSDDLKEFSKLHQNREQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M931</accession>
    <name>A0A8S1M931_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0480050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8071784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M931"/>
    <dbReference type="OMA" id="EYDFRNA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035799115" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="220"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8071784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="42115" checksum="F3A979862B773FBD" modified="2022-10-12" version="1">MKIFALLLILVIVSGTNHQQRLNYTKQRSITSVMAEVEMKIKSHSPLDAVLNILTLFRDSVNEEQVKHDQIFNVELNECQSEYDFRNAEIQNSKNTFRDSNAQLKACQYSKQRTINQQQINQQQENTFQQHLNTILQTAEQGYFKKRSRQYEDSLNSIEEALTILEGISFGYRSFSELSKVSQRMLQTSFDINKTSIYAPIFNAFIQLANQGESIDLSLLQQVEHLLNDLKSTINNAYNQFIESNSQSVALFNLQKEKVNKVLQRLQQQYEKQQIKLDKLQTCIGVQQAVSNTAQSKQQRNQQLLEQAQALCSTFQTEYNYGTQSRRNEIQLVNQLEQMVRQRFEEVHEEKIVLKFAKLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAJ1</accession>
    <name>A0A8S1MAJ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alpha-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0580049</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAJ1"/>
    <dbReference type="OMA" id="ANVFRKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="5">
        <location>
            <begin position="678"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="85"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8077310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="842" mass="100184" checksum="5A5FF4822DB8D816" modified="2022-10-12" version="1">MNKIGIRYPDGNINSFEMQNGEVLSLRKIFLNHAQNLPPLEVTILINENTNTLVSRDETLKTNVPYKLVDCREMIKCFNSLRDCINQKDKQLLEYQQIIKEKQQLLDSQKKVIGDQEKQHLNDIQHKFRDLESNFKQVLDKNKDLQKENETIQKKIISVKEKNKKFQDQYEKYQEIRIKITDDKSAAEKQVACLQLELKHKQEKIIEKENLIKCQEEQHQKNLEQQSKYFDQLKFNLENQIKREQERLKEVEQQYQIALQENSALRQNQSSNDQSNKSQLFQQKDLEMEIQQQKLNFEKQINDKDDQIAFLKNQNDIKDMMIQDLKLNLEQINKESNQQKQQLQNDKQQLQQLERQIEEQKCQINNLNSIIEMADQEFIKKNGYNGQNQQDIIDNYNYRLRRLVECIPRFYKEEINFAIEEMFQKKKYEKKGNCINQTFQVYQLTNKNTRDIIFTQSNLDVKQTIIKSYKYNIHGELSKEKIVSEKKYSDKSRLIDSIQLYYSKSIDTLNDEYIKIKSENSNKIYMIKETFTNQQNNIGDIDYTLHLLSRSIVAQMILDEFVAELKNKNINIPFKFQFVQPVLFSIKIHFKEYFSNLIAKQGEKQYTNAFKTVISFMDIIEEQYSNDFYEYIRELQLTRLEVKSKDGKITNESNKEDQTQFIVKFIYTCYEDKKRDPFKNILLQFNLCSNEEKLKAFIDLTELLSETYNTFPTEKFFYGYELKLNDINDKSVFKKYNGGNTSFDSSEEGKFLSALSFYSILKTEQQFCVSHIQGIGNYFYDPVVSTYDGFLDCLDQGINEIRNIESAFSSSDPKHIGFKYIEALGPKYLEAMENMNMNEQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MC42</accession>
    <name>A0A8S1MC42_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0540115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MC42"/>
    <dbReference type="OMA" id="KHIQTNR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8075263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="243" mass="28029" checksum="B8908BB1E8B1B9A5" modified="2022-10-12" version="1">MLFPELQSLEDFEYIKNLGSGYSSTVDLYKHIQTNKIVAVKKLSIKNLSQSECRKTYDTEITILTKLKGCSNVVQLIGFGFYTTIDNSTRRTKTLNNYFLILEYIDAQPLDKNNERITKQFYYKLLQTVQKIHELGVVHRDLNLNNVLVQNNEPILIDFAFGAVTNGKQKFAEFYGTKPYVAPEIVDGQSYLPYPTEVYSLGVILLELINNNESQEIAAQMMQQNPYKRPNLEDVIAHPYFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ML43</accession>
    <name>A0A8S1ML43_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0620248</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000063">
        <property type="protein sequence ID" value="CAD8079882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ML43"/>
    <dbReference type="OMA" id="MRFQINI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="133" mass="15787" checksum="941E0522FBAFDFD8" modified="2022-10-12" version="1">MQVQKKGQQQSFAEYVKQITSQSVNRFQQTSNKQFRSTNLPQLLSDMDRSPYMLTSLDKAKFPQVTKMNKTSYLNYSSSEQKNFEELASKFVKQLEQQTLRIDKDTPRINLMRFQLNIQKSKRHLQRKSPMPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MNB7</accession>
    <name>A0A8S1MNB7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0660131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MNB7"/>
    <dbReference type="OMA" id="MCKQENL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8081710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="281" mass="33139" checksum="1B52418DF4A77251" modified="2022-10-12" version="1">MYQQGNNYDFKIILGRVKEKNMSLRLCYSQTKSEDYEIVVKLKVKSNIDVKTADSEFKERCKSTYTILTSFVKQIRDFLKPTFALNYHVDEHYAYITFTYSNQVELQEFMEICQFIRETMERSQEKEQVELLIESDLSFDKLSESISNAVYQMNIEIQGKAEFQKDTVKLLENLKMCKQENLPFFLQQKYLSQILIYLQLLQKAHAEVEIDKIQYDLGFAQPSIELPFKFAIYGKLQRFAEQYLDQGIELIIKGPGLSVEINYNYKGSLFKLIDNILIIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQC3</accession>
    <name>A0A8S1MQC3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0610146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000062">
        <property type="protein sequence ID" value="CAD8079146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQC3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="153"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="251"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8079146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="363" mass="44158" checksum="F4C3FA11CB81BE30" modified="2022-10-12" version="1">MQRNISLNNSKQIEIKNKRDIRSKKNDANDIFNKRIIQLYNLKEFIGSVAKNQSFQQQTQDLQNQIQQFNQQTQYQPQLQQEINLLIQGNLDILQQQFDTKTKDFDQLKNDLERYKNRSQQLKEYWVFGKVNIKSQRNILNIFMKQKKTVDLQKQIENKRLEYEKRIIQLKLQAFVRLLKDQEQLETKKQNQESKIYNIKQQLNEKRRIIQYLDLEQDFINFKNQILNLMMKEIRNSRIGKQKFSLRKLYLENELKRLHAIVIQLQQENNELKSRRNQINYNDKQRYKISNNQMLQIKFGINKGNNINNKQTIKFQDNNNKFSNCINNYNNQENNKLIFEIRHLKKKTSTQRNDKILKKIQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MUX1</accession>
    <name>A0A8S1MUX1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0650058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MUX1"/>
    <dbReference type="OMA" id="MPEISHK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8081116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="315" mass="38254" checksum="4A5BAFA58FA0EEAD" modified="2022-10-12" version="1">MNNLHDHQTQSLKYEHMIDFNMQNIVGYILSKDLKYIVIFTHSQQDIQNLQIRIYQTQSFQLIITFNISQINLKIDKIFFSDDSSYLYYYSHFNHLNLLNLEDLTFKTIFIDIQSKNQKHYFCGLTLFIKEEQKLHIYNIQNQQKIEQIRFGFPFSHFIPFSSNQYFIMNDIQQTLEVWFNYQYYFKNKISKQFSKIPNKIVLIKNHLILCVFMFRSIQFLSQNNFHILRNIQLINYFMNQQLYIDDKITIIRAQIHPPKLEQINLFPQISNNQLLLENDFLEQFDIHEQTSKLLIGINFQESANFKLKIYKLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWP5</accession>
    <name>A0A8S1MWP5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0650213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000067">
        <property type="protein sequence ID" value="CAD8081385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWP5"/>
    <dbReference type="OMA" id="YGRESLH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="735"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="735"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="764"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="774"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8081385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1131" mass="131935" checksum="BD9129560AA95546" modified="2022-10-12" version="1">MKKQQTNSQNSSVGFNRTQVFEQKAEQSNPFEKTLKSMNTDRKALIQNFLDANANSNLEQHPHNQRVFTKTITVEAKVDEIKFDNIPGVLQQKKFNYGNVYKERSKKNPYVIIQSLVRRFLAKKLVNERKKYFQKLLKEDQKITIVDKWVNDEKNWAQSVISKLKVQPKQKNQERKTISGLDKNNPFYNTQIEIMGEMGVLEKEDFIEINPATAFANPAIPITEDIKIKKPPLTRIRPKSAYQSQVKPKTMPIQLPNDNCQIWKALYKKKYGRESLHDLSMKEIKITDDYIKIENQEAVRDKARINKINKILEKEVPKELKVQKQKQTTSKKKQLEDSIEDEVQSEQVQSEIADEVEEEIPEEEDIEEVVEEEGVVEEIDDIQSSQIKEESIVQESSVIRESIPSAQKSSKIIKESIQEDYGDDFEVEESQKIKTQQSQYEDDQFDSYHSQIEGDTHRRKATPKSISDKAVISPQTFVPEVKSIPIEIDPGWTTKRANQEAEILLKNYREKYSQLREVEINDEDFEPLKQEHRDLKFIQQQVNNLISHTGDFYKGWKDAVNPQPSQSADKLYAQFQYEFQRLLSMNSLGTRFSKILSQTIVKDVPVGNVKNIMSGIEQQMSIVQQVLENLLFQVQQIFDFKQTMEEMRLYRKEMDQKLQWLLFKQDKLINNQLGKQLEERDKDLKKFIAALFEKQRLEFGSRKLAATVDPHSLSEALLKNQGLLSYKFEQSAQSKKDSSYGKQRDKRSVKEESYYQADFEGEESERIKTEKSDQNVYEQSYKNSLTEDREVEDGSQQSDIDWQTDINLFENTQNSFVDKKDLDVQQIVEYLQEQLLKEILGDPFPIRGQLQNSQVKNESNLSSQYSQSFEEESLSASKEIVPEPKQPPPLAVKPIQTKEKVDDFGMGKSAINRDELSKEFDLSENSQFLEKSENKEEGKEVKLVTDFRYQNFVKQILDKYGKVLEGKEKQIITKFQQIVKKYKQDKKKTLGYLFSTEFKFIDQKQVNEQNAFQIVQSLEEQLCEQLLKEYNDSSKLDIKSAIEQAKVVVLKITEKRIPVDAFSSSYNFQEIYNHAQRKPPAKECPINQELADVYINEEMDKFGNEATQYILKQIAKNTLADFDKILLKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N288</accession>
    <name>A0A8S1N288_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0710045</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N288"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969">
        <property type="entry name" value="RING ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="206"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8083886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="261" mass="30831" checksum="468E0346C32B8C47" modified="2022-10-12" version="1">MEFANGCSCYNNFIGNDCNQIAQELLLETQKALTFPTSYLITFFYIDLEPIKEQTLRLHFSLPEKYSEELKYLIEVRFYRSKALIKQFFNQMHYYVESFFIENKNLTITIRIPSNLTAQYRILQFAILKEQITVDSLQLKIDTDYEEDDNESDEISRLLYIIIPTVVGSIIIFLLIIYYVKKRKNQAANYQIPAPINNIVNEPEKCGICLEELNIKNTAVVKIQCDHQFHLHCIQDWAKKQMNKQSDCPYCRKPFNPNEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N5F0</accession>
    <name>A0A8S1N5F0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0730078</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8084966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N5F0"/>
    <dbReference type="OMA" id="SIAQYKW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF140">
        <property type="entry name" value="ZRT (ZRT), IRT- (IRT-) LIKE PROTEIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="321"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8084966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="344" mass="38221" checksum="5FF1D316F7F9AFBE" modified="2022-10-12" version="1">MSDDDSNQVILAITKVGCMIAFFMLILIVGSLPIRLKAFKSNKKLLAYMGAFSGGLFLAVGLVHLLPEAADNFEQSFDDDEEHFPFAYAISILSFALILFIEKIITDHHHNHGHDEEHHHHDSNSKNTQVQDQNQLFVNGSVDTEALLEKKNTEDTFKDALNTQLIVAKKASFVQMVKKSIAQDPKNSMVYQDVNTWAPYILQIAIGIHAVFEGLSIGIQEEVSLCIGIALVVCCHKWAEGMTLGLAFRKAGVNKTTSTYMILIQSIMNPIGIGIGWIMADKGPLYTGIFVSISVGTFIYISTMETLTEEFSIAQYKWEKYIIFLVAIAFVSSLWFIEQAVGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N7E9</accession>
    <name>A0A8S1N7E9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0810113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N7E9"/>
    <dbReference type="OMA" id="KRMEMAF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="75" mass="9185" checksum="0203B60EEEEAB7F9" modified="2022-10-12" version="1">MVNFTISLLNVYKLSILMQRLQVNRHQMFLGISKQKLQKMVKNRLFIQKRMEMAFSMKNHVNQYQINQRIMLRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NAL1</accession>
    <name>A0A8S1NAL1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Opioid growth factor receptor (OGFr) conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0700178</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the opioid growth factor receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000073">
        <property type="protein sequence ID" value="CAD8083674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NAL1"/>
    <dbReference type="OMA" id="KTHYVQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140625">
        <property type="term" value="F:opioid growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006757">
        <property type="entry name" value="OGF_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039574">
        <property type="entry name" value="OGFr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14015:SF2">
        <property type="entry name" value="OPIOID GROWTH FACTOR RECEPTOR (OGFR) CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14015">
        <property type="entry name" value="OPIOID GROWTH FACTOR RECEPTOR OGFR ZETA-TYPE OPIOID RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04664">
        <property type="entry name" value="OGFr_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Opioid growth factor receptor (OGFr) conserved" evidence="2">
        <location>
            <begin position="25"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8083674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="264" mass="31749" checksum="F8809342CBCE311E" modified="2022-10-12" version="1">MGMCESREELKDTQFGRSTIQPKMSEYPNLDFYKGVYPFQLNNQIQFHIKHWKTYNQPYQWIKFYSDLESDKQFMQWLYPNFFQSFLNTTSFRLSIKERNSFIEDESIMKQYLDNIKMFQRFLGIGRDESQQLQILDQQQFDQCIYSYTQNQVRIQRFISSMSILGQRELALELLQVIKNKLTKKNKIYQSKFQGFELLVEELDLSAQSLFSKKKPTKSFGEYKTHYVQEDLLNEPWVQASILTKAPIIVIQDKRESFEIKQEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NBJ6</accession>
    <name>A0A8S1NBJ6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0780166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NBJ6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8087371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="214" mass="25220" checksum="E9735A2D95CA22BB" modified="2022-10-12" version="1">MSYYPGYNIEQPMAYPNQYLKRGSYASVQYSSPLTQSVLPRMAVQPVIQKSYIVQPAVVQQVMSSPVQQTIRGESKIQYKESQRQVVEMETETVQVQVPKTKYVTNYYPIEYQTRYIPRTVYEQQTKYIPITKSVPRVEYEAVEREIQRPQLVEPTQYIQQPVYQSYVQQTAIQSFNQQPLQYLRVRPEQSYIPSYNNSNINSYPVNYGGFRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCP8</accession>
    <name>A0A8S1NCP8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0780121</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCP8"/>
    <dbReference type="OMA" id="DTIYYMV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052234">
        <property type="entry name" value="U5_snRNP_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006:SF1">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="PX" evidence="4">
        <location>
            <begin position="3"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="429"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8087283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="533" mass="62232" checksum="72D693A1D3BFA858" modified="2022-10-12" version="1">MDSKSPLSASVISFRNDPNDDTIYYMIQVKNKTSDIWQLEKRFSQFEDLAKKLKVLFGEQLPTLPKKKYITFLVGKTPEDLEKRKAGLDEFIQNLASRPEVVASEPLKQFLEIEKNAKEIIVNPPKQIFEFKGFLHGIRDFTISSEQGLMFVITSDCSVMNRLDAYLTNMKAPWESEKGDQALIPVGCVECWLQQESGEFQRLWVKMYNSQAITCYWDATASVLLVGLDSGSINYLYVPEKEGFKKFTESMEIEQHTDRVMGLYYDNKRKYLHSISKDRKYRVLNLRKGELVADIEPDQYELTCLLGSEERKKTFVGDRHGEIFIYDIEKKRPKLIIKIPTTQLFIRGFFIDNQRNYLFSISHENGVILIVDIQLPGREQYAKEVAQLNGKSKSREIGWSSKRGEIYVGNIDGTVTIWDARKSQQLYVLKAHDSDITKLQWLDADQTLLTAAKDKVIKVWSMPQYWRDPKIVEKELEQDQLQAKQQNVEQFYKKAQQQIDQKFQDDDDDDMAVWHKDQQRYSDTKYEDIIELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCU1</accession>
    <name>A0A8S1NCU1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0770130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCU1"/>
    <dbReference type="OMA" id="EVATIYV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="88"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8086855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="45046" checksum="32DD0B7772365094" modified="2022-10-12" version="1">MSDNENIDITNLSEGKIEKYIQQLETSIQKRTTMYGLDDLTMMRQNQILCKLLNQQGLIALQKQNYEQCIEYLRKAELATIYVPELKVQTFNNLACYYRKMGKTRTALQYLQQALQIELQQKQSTSLPDIYLNLCAVLSQLERHEEAIQHIYLSIIMLQHELLISQLQKPEQKQDQSNEQSFPSRQSSFQYVNTVKSHYNESKKIQERMSILVVAYHNLGVEMEHLKLQFESKKIFQSALQLSEQCLPQEHQLRHALEEITKKYNQEQQQQQQQVNEQLTLKPLLPKVHQKNSELKQIYKSVSPKQILRRNTQLQQQKINPNKNLKFMKKNSFSETYRLERDRIMSINATAEVIIKNQENKDLSNFPYLKTAVDTVNQTSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NCZ1</accession>
    <name>A0A8S1NCZ1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0780167</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000081">
        <property type="protein sequence ID" value="CAD8087373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NCZ1"/>
    <dbReference type="OMA" id="CITIVYD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="405"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="537"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8087373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="928" mass="111146" checksum="6932735EB13EA67A" modified="2022-10-12" version="1">MNNKCELIIQESGLEMERSLDHSIIRSPQAEQLISSSNKQTLLKFDSSFAQESFQKRKDSLSSQNLSDKNQMITTIIRQKQFSKLLIYCHSMKFANRLISLIKPCSKFSIEQFNMINDKASFFNNNLQTKKQTTKHFVQQNLRAIDFQFRWRLQLRKFKKRLVEYLHYQYQQIPLIEPESQKLFIWDILLNLIRIYLIIWIPIIMVFQNQQLEFNNRIMILFSSILLLIDVFLRCITIVYDKGYPVDNRYLLIKRQIKLSNLFEVCSIFIGIYLSIRFSDPQFNYSILDENGWIKLLLILFLIQIKNVVKFMNNMQYYYKPNKEMSSVIDLIKLVGLILLIQHLCSCVWLIIGMNQLTQHHQSWIYKVFLESWEVQYLEAFYFMSVTMFTVGYGDINPQNSLEKVFCICYMFLSTLQLSYSVNTIGAILTLLKEKNEIFRQKMTCMNEFLKDKNISQQLQYKIREFCTFYWEQDIIQKKNEQQLLIKELPDELQYHLKLEQAKQLVNKCTFFKQHFAKSTIQYIIQRVQFTSYQPGTIIQITPSNNGVFVIERGNIEVLQNKTKISDLKDFDQFGFQEYINNQYNLDIIYQTTTYTSILCIPYQILNDAIKKSKSDFEKIQIKKQITKFHFCYICKSTKHSIEQCKLVHYVADKEKSIKTYLLVDNQPRIKVKRSNRFQKFQTLIQQVCLDASCKLIQQEYEREIEQFLPELTYEALIKPLNSSQKIFESPKDNEVYRPQKQNYLLETIKTSQVRTYDKFCNNLSLTFRNQDISPFIKEPDILKELQLKMINIQKYPQKQQKELFILYKTLENQSQADIGIGNFEKVIKFTKFDRFWNISNVIEQLNCKYEHSRLYLDIIKRLSKYLLFPLEFIYLFKKDLLKQRAESYQNRKIKDSDDEKSDNKQISPFRKFERRKALIYPKSLTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NE40</accession>
    <name>A0A8S1NE40_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0870018</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8090908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NE40"/>
    <dbReference type="OMA" id="QIRSCKL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035907714" evidence="2">
        <location>
            <begin position="16"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8090908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="317" mass="37793" checksum="0E90C75E1C9FC7D0" modified="2022-10-12" version="1">MKYFLIFLTFSISICIKLELLEVFHKSIDWQANYQPTYEKQMKKLERQISIMKEQRRAKNQQCDRIQRGLDFIVNEKEKNEKFTSWLTSRIVQNQNRVEKLNQIKCQSSSSFYETMNDQNRVNKLNQFFVSWISRDQKLLQLQDTQAFIQEMQIENEYQDALLILSEQIRSCKLSPFKQQTIDRSESSRRDFEQNIFDLEKLLNQNNDQMELNEVRVQDKITKFIQQLDKETEQLQIHYESIMKIINKSPVETTSKQRLDQCKKDVLSIDKLIDATQEAIKRIQTCAPSDGLAFKQKRILSQIAKEYSQGVAKFEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NG18</accession>
    <name>A0A8S1NG18_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0810046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NG18"/>
    <dbReference type="OMA" id="QKVAHME"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8088443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="266" mass="31174" checksum="D82FB141D0688542" modified="2022-10-12" version="1">MQKIKKSKYRLKDVIEGKTSNDEKSAQKFVQLSHIKKNVQEFNELKSTLQDRKVTFAKFAFRQQELIDDKKLGPVLKGICTRTRIGSMQITELIPKFDLYKFLKQAHLEPSKRVLRRYAHWEWDQEQEEKKEVPPSYKFTSKTEFEYKKKLETKDAEKKLRKDIIKKWASSLPDVAGLTAFGKQFTMQSSPLRSTKTTLSKTHTYFSPNPRSESLLFVSGLIHELDEIKLDNLKIRKKLRGTQSSLQKIIEKQQQQKVAHIEINGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGA3</accession>
    <name>A0A8S1NGA3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0810136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000084">
        <property type="protein sequence ID" value="CAD8088533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGA3"/>
    <dbReference type="OMA" id="MINEAKM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="158"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8088533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="263" mass="30791" checksum="C3A364824F810794" modified="2022-10-12" version="1">MNSVPFQDILNKTNKTRCNSPYVSSCSSKRQSAVYSSNSSYAESNQKTKPDERTINQLYSLFKKQGFDNINGLLKFLDAQRDQQTRKSTSPMLRTVQDENDRKKFGIMINEAKQGLNQLEHKIINLQNKQECRNNMTLKYKNLLTEDDENHEYLYENRQDYKKLYTQTLNEKEQLLKKVDQIVQENKQRDEKINSLNQEVELLKKQIQGLLILKEESSQSPRPSISGSDYTFTLNVCNSEVRGDKPISYKDFLHTQSQQIKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NGT0</accession>
    <name>A0A8S1NGT0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0800139</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000083">
        <property type="protein sequence ID" value="CAD8088205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NGT0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014560">
        <property type="entry name" value="UCP030333_Alba"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947">
        <property type="entry name" value="DNA/RNA-BINDING PROTEIN ALBA 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947:SF36">
        <property type="entry name" value="DNA_RNA-BINDING PROTEIN ALBA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="2">
        <location>
            <begin position="24"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8088205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="115" mass="13130" checksum="175CE26029D5205F" modified="2022-10-12" version="1">MDRQQQQNQPQQQTQKQKLDPQDNHLQVSIQKSLKAQVFVAKIFLKKFGNVELHSLGDASKNSVKVADTLQRQGIVTITKINSFTLDINEGKKVKFIVSLTLTQEGKRRLDQELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NHC7</accession>
    <name>A0A8S1NHC7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0820132</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8088903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF67">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HUWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="3">
        <location>
            <begin position="1296"/>
            <end position="1629"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="2">
        <location>
            <position position="1596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8088903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1629" mass="189786" checksum="445C09E9CD56E13E" modified="2022-10-12" version="1">MITTLNILYQVATNLLLEKTEPVITNSLSVNQMELGIIGEINPIEINDQVFQNDQTYQRYYNNNSQNEQFQYYLQNWPFLSIKNGQPQVNADQQMEPDIQIHQMLNIPLINDSQEIFDKELEEELNYWTDDVQYIIEEEPENNQNIDYSYNCFINGISDQLENYIYRTIKFEGLRFPNQFQIYLQQFKQILPSQNNAQLMPNFNPWQLNLYLENIKQLPNLNRDLNQNMDYNFGNVQNHNINNNGVDNDEENEDMSDQIDFEMGLGYNQGNNDNIQMEYENQQQQQQPYNQPLRQNNQPQFLQIRNIVNSVLNNRKQTLKHLGKIDQQLIQFLLKFLYVDDNFGSNYDYINTLLISLGTKANFNNQIIQAAINTLKKTTQNQIQENADQQTFVFSRNGIIRDFDYSSNFIQNKAFKLLLQLQSTSKWNISDENIKDFIELLFKLKGNSLKQLLYFLVNQEQKYKLILAPEQVKMLYNFMIEQDDIQNSSNCMKFISNLWNVDKNAYRLMSQMKFQIISISEYYKNQFLNQQQVEQTFKQLNEVEKIFQMIYIASKDEQQLEQCRNFFNSQEIQDLFNILDQLIIQIWNLHDCRYNYKLLPILKAFLICHQIIHPIANQNLQLLKVSSMEISESTGNLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXMKFQIISISEYYKNQFLNQQQVEQTFKQLNEVEKIFQMIYIASKDEQQLEQCRNFFNSQEIQDLFNILDQLIIQIWNLHDCRYNYKLLPILKAFLICHQIIHPIANQNLQLLKVSSMEISESTGNLIDFERLFKKLCKNGREYINQIVKEQINKMITYYTTTRQNQQIRNDYLFEKDFFDGILTYHPQVIDFKNKKEYFYQKQQINGNNQQQIFGGGRMFRISREQIFEDSYRQIKSIANPNILKERFQIIFEGEQGQDEGGLTRDWIYKLSEQIVDQKYKLFVPNSDNTCFIINRESSSDPDHLNKFKFVGKIFAIAILRKVPIYGQFPISMFKHIVGQKLSRADIQNYDEGLKNTFDLIYNQRIDSSWNLFWAYTIKQGGVPDDVELKPGGKVIEVTEYDKKDFVRKYCHQIMAKDIEDQIKAIQDGFYSLIPKEDIKIFDSIQLELLLCGESNINVEDLIRTLRYQGYTAQHQVIGWFQNILRSFNQDMLAKFLSLTTGHPKLPVGGFQNPECRITIRLPNYLQNDIDSRLPTGHTCTRVLDLPAYSNEKTLKKQLETALNEGGEGFDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJ41</accession>
    <name>A0A8S1NJ41_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dickkopf N-terminal cysteine-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0860049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000089">
        <property type="protein sequence ID" value="CAD8090466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJ41"/>
    <dbReference type="OMA" id="ILPGETC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052326">
        <property type="entry name" value="Diff-Dev_Assoc_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33459">
        <property type="entry name" value="DD-GDCA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33459:SF7">
        <property type="entry name" value="DD-GDCA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035719875" description="Dickkopf N-terminal cysteine-rich domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="37303" checksum="5D9FDA1DFA9C038A" modified="2022-10-12" version="1">MIKELLLLLYFIILVYAFANTKCGGKRYKCGEENQNNVCVNVSEYRGKVHELTPCSDDKICLWQDAVFQKPVYCTDKPTKDKILPGEGCSGDSDCLSNSCKSGVCLGLKLNQQCAGHQYCDVGYYCDTYCKQQVQFEQSCQNDYQCTNNCVCNLGKCAYYYSLENNIKADNPKACYYGYINPNNGTCQNGPHSLTKSKPCETDTDCILLDSDQKLYGYSECQCGFNAGGFSYCSLAEGDPEYLKILELFQWLLQVNQYCHTILRYGPCSSLYLDEYIDYQKAVKFYELQSQIMFNDECIQKIYTDEYWGIHSNRLYILLIILLLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJA3</accession>
    <name>A0A8S1NJA3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0770065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000080">
        <property type="protein sequence ID" value="CAD8086724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="YIGNMLY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035852647" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1670"/>
            <end position="1693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8086724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1741" mass="201634" checksum="041BB157B265D88C" modified="2022-10-12" version="1">MIIFSLIISIAIACKSLSESESINLENGKKQELCFKVNTNLPTIVQLVNYEDEGSKKKKKKGKTNKEEEEVDENILIYEEVSLGQCKLKQGQKICYFEQGKDKLEVDLKCTKGPCKFNLIVMQEEIKQLQIGAQQKSKLKLLKIAQNSNYRVVYSIKCNNCVMKLIDANLYYYGEQPTILDRDTRYIAVFYDGIDSETIMVLTGEQEFEIERFEYVKQQRLNINQVMIDALEKSKKNEYIIEGLKVIKLKSHTHDIMKIKVKNQQGEVILDQIIDTFQHQNYNLLWNEDFNTLELSSSKKISYQIEVIDQTDIITIESSDLAMGQQYGIVNYKLKLPNIQDNHFTVSADSGEDVSVQVKSCKGNKCEAIEKIKNGESSQSVEQKCDNPDKDKFCWFVIAINTQGIYSLNIDDNNEAYMLTENQMINSEIDEEQKHHYKFNLANSQNYSSVIFRVTSENVQILISKSSKCQLVNWKCYEYSGTSEQPIILSDSQLKDGQYSITIEGMELAKYGFIIETHPKDKINIVNIKKGQIYKSSINSNQKKQCFKYEQSTKGVLTLLIHGPNQKLISYANIDTDDEIPTKGDYDVITGNSALQLVSDRFIACVEIQVQNNQDVKNETYYNDIKYSLILYDAQSVINLEFNSAYYGQVAFTEQQRFSFLSYFDEDVVYITKNILSSGDVNKELQVYIGNMLYENLDQYDYKFFDNEVSTFSLSSEKMKKLCDINEDESISVDHNRITHLCEIFIVVKSLSKTPVRYTLTIHKQDLAIQLTDGLQQSYNLQHIEEFMHFYYQPNSKSIDINLFASTFYGKFVLHINIWKLDTIKHKSDWPFIDKEDDSDISSGSTNQIHRTIKSTALDQCWPDCVLLISIQSVQDSQLNVYNYNDQFHIMINQGMQDVIEGQKYEVSIKSEECVYFTFKNLQKFRDDAYLTFSLLQIIGNGYFTITVNGGDKFEYPNLYGSFDFHADGTYFQLSKDKLNEKLKEKEISIQDAYLIISISGLQSYYNSIQQSIKLEFTISYMNMDYIQIMHSQPNTINAKYHQPTIVQFYNYLNQDIFFKLHRFLGYGSMSVFICNKQSNIAECVKDSNDAEFKYLDTVLAAGNENSQILIKSNDKTKFCIYCTYIIKIESQESELHGQLTVVLDNDFVKLPAGVQYSDYVEKQSSSQFSIFFTQDSKIEIFIQIFTGDPIVQYSFNQDNLNSSKIIKAQPNNKYIHITVPSQKLLNDLEDIAQGLLNKNKNKKQEDEIEIGLLPSHFVHDELFLKVTTKSNDPCNYTIYYTSDFVNGKLQDGRIHLAIIENNITFLYENYDQQISVLNLSPLNQTSLQDIEIKVLYIKKQSFDDYHYEFLEEIPIKTRLIHPLSSSYELPKDNGIYEIILKNKKYIENGEGTNPTQFIEISASTNDIRILPIQRHHSGYIQPTEFDYYETYAPSEGYLAVEIYSCSSDINFSFSKADIKSFIDEDYDESVDIPEKDYHVEMISVDKGTLWIAIKGISTSPAYYHITNHFYQNKKDIPFGKIMAGNDGHIKWHLDKSKADSIQIEFEHATCNKCNLDGVQINYHIFIAQDEKKLQMLGYCGNEQFENHKADDEKIFKKSIQSYRLADHNGSIVYEIEFPNLVTYEYASVGVFATVDNFMNSTSQIKVFYRTIQVPVPNYFAKFLNHNRTFFTFLIFGMILLIALLTLWLCYYIKRYRNTKNKLDFQLEQGAKIIASSRLPQNDDAAFQNDFKMKYQTFEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NNT4</accession>
    <name>A0A8S1NNT4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0950024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000098">
        <property type="protein sequence ID" value="CAD8094148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NNT4"/>
    <dbReference type="OMA" id="DSNCWKE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="153" mass="18036" checksum="EF6D90B9B4DDCFDC" modified="2022-10-12" version="1">MYPQLQKEELVEFRNYIREVTSAKINKTLDKTPKRNNAQKQLEFILKSTQLRQTVQKPENTQQQIQRINYESQSYIPRSSSQPFVHFDSNCWKETQNSFTKQQTAYVSVSEMLRITNGFNQMNKKDISGLNSNYLQSLHELDLTITALLKRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NPA1</accession>
    <name>A0A8S1NPA1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0870025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8090915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NPA1"/>
    <dbReference type="OMA" id="MELINIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8090915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="44754" checksum="74F603210A996F94" modified="2022-10-12" version="1">MFIPYKKNKGYKIPVIFEFGQQNTRVGYAGTDGPEVVLPSDLLMDSEGNMKFIEQNSTYQPVGFDVKNILLGGQIVDFDIWKEYVEHILNNLLFQERHFPFFFVENGNVKKETRNKIIEMLFEEIDVSSVFFHKNTMLAQYIVGKDNVLVVDCGASHTTITPILDGNVIEKGILRSDIGGEYITNKLYEIWKQQGLAFSRFKNYSQLSPSLQMYGDLEIVREIKHQCVKLYDQPIENLKFNPTVDNQAYELPDQTKLLISEEMYKYPEFLYEPTENFEGLDKALETSLEKIDSDYRKELVQNVILIGGASCTVNMVERFQRTINNLKIFGLSSRSKVQTLPKFFDRQHASWLGASIVASSQIYDQWTLNKSDYDEHGSSLIEKKMFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NQ22</accession>
    <name>A0A8S1NQ22_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0960080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000099">
        <property type="protein sequence ID" value="CAD8094558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NQ22"/>
    <dbReference type="OMA" id="KGGSPQH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8094558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="221" mass="26693" checksum="5A113430BBE61C45" modified="2022-10-12" version="1">MNTEQQECLPIKKFLLKKQISERLFNSHIHSPQHDQQRGSPQHQRKIIFPQIQVPQENTDQKHLISPKYQQSVEKMEYIKLPEIDEKFYIKRSIQQELCFNVKMQHSSRKNSTENNEIVDNDQFILPHHQNNKKVEFQQDVLVYDYINQTYKKSNIDGSQKPLFRRQKTKNLSDDLRLYNDNFKYNGKSQFFDISTKEKHNQKHFIIDTLQMENENQINKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NWD2</accession>
    <name>A0A8S1NWD2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adenylate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0940090</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0940091</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000097">
        <property type="protein sequence ID" value="CAD8093855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NWD2"/>
    <dbReference type="OMA" id="EDHLLYD"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8093853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="26079" checksum="B7C856864D2B8262" modified="2022-10-12" version="1">MNQQFFGNSRLAIQRAQQLHQFFTQSVIYDQFKYTAKMPQYPKIVFLQSNQFFRNLVEKQFIQYSIIDAQKSSGEDGLARLAEQVQLQSTQTNGALLFGFPNTTVEAERLDRLLDGVNLAVRFRLSESLIKKIAGSFLSCQSCGKVFNTSLPFITPTHPGYQNNCQTPSKCALESSQAPIDQVNSEIIEYYQKKGAYLEYEINEDHLQYDSQEFFEKLDNAVASHIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NYC7</accession>
    <name>A0A8S1NYC7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1010067</name>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1010069</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NYC7"/>
    <dbReference type="OMA" id="LWIILQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8096389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8096393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="149" mass="18215" checksum="0D7AC80F5338C93C" modified="2022-10-12" version="1">MLQQMRFNFQQQIRKSKIEDQLKEARKKYYNYGDNLNEQIILNQNSSSEFQQKIQYELKNLKKQIEDDENYIINLAKSRFLEEMATPILIQNYSAINSIFFEIFIKMIEFAKRNNLFWIILQFRHSQFLNCLQSLEYSNEFQRFINSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZ38</accession>
    <name>A0A8S1NZ38_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1020128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000105">
        <property type="protein sequence ID" value="CAD8096839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZ38"/>
    <dbReference type="OMA" id="QDITQYC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="72"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8096839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="340" mass="40288" checksum="57B05A03CD8688F9" modified="2022-10-12" version="1">MGCQIGKTNQIQIRSKYNNLIGIKFQEAIESSNPQLKYLQNKWNQFEIKDFTAALKFKQSDPTRFRKYLMNGALSSNRWPLWKVSLFVDNIFESGCYEALLKSSQNQFDSDIEKDLPRTLPGHQEFHKKSQALQNVLKAIAVYNNDYVTGMNQVAGFLLLVCGMEEEEAFWMLNYLNINPQFKFHQLYQTSDSQNQRNQFEFVEKLLNIFHKHFKNQMPQLYQHFEAQDITQYCYIWKWIFSLFLQTFPFEVVLYFFDFMIANNILAIISLSLALLKHFQSHLLKLDQYQIAQFITQLSECQDVFDKSKHSNHLCVEQIIIYASQFQEQLGFSRLEETLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P242</accession>
    <name>A0A8S1P242_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1030129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000106">
        <property type="protein sequence ID" value="CAD8097233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P242"/>
    <dbReference type="OMA" id="QTMNFPL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="116" mass="13768" checksum="F3E8B998A3FDF010" modified="2022-10-12" version="1">MFEEPIMESMLDHNQHLTLDQKLQTMNFPLKTLSEVKEKYKYQIDYSTLQERKFNLRKTRQPIIIFSSAEIEIPLPRTEVRVKSVISPEINQSLLFVSVQPNNTKSRSLGFRKKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCF2</accession>
    <name>A0A8S1PCF2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Papain family cysteine protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1130145</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCF2"/>
    <dbReference type="OMA" id="MSIYLKW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035786597" description="Papain family cysteine protease" evidence="2">
        <location>
            <begin position="24"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="4">
        <location>
            <begin position="36"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="3">
        <location>
            <begin position="119"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8100880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="36537" checksum="7577873199108B44" modified="2022-10-12" version="1">MEKIFLTVGLMMLLGASLYLNNTQEVLDEIDTGNLYTNWKMKYNKRYTNQRDEMYRYKVFTGNLNQIRAFYESRKETTYTLELNQFADMSQQEFAQTYLSLRVRKTAKINAANDNFQYKGAEVDWTNDKKIKYPAVKNQGSCGSSWAFSAVATLEINSDIELNKRYVLSEQDLVDCTRPYGNQGCKGGQVDQAYHYVLDYGLTEAKDYPYTGKDEECQSSANRPFQHLAGYYLYETCSQLADAIQKRTVAVAVDANSWQFYGSGVLSECTKDLNHAAVLVGVSADGVWKIRNSWGATWGEAGHIRLAGGDTCGICADPSSPVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PCZ4</accession>
    <name>A0A8S1PCZ4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1130135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PCZ4"/>
    <dbReference type="OMA" id="NRFHDIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="563" mass="65827" checksum="4F0DCB0CC21D5CE4" modified="2022-10-12" version="1">MNELKSPKLLNGLGQKKQQNVQDQNDTSLLLQLFQKIQPVEIIRMNDSKKKSQSVLYIYEDESESKFQETIKYGVLEYEQHQYQQALFQFKQAEQILTAIASPPIHYQLINKINIGWLENAFTCFEDTILILKKYINQTTQERILLDTIRLQCFLQCCIILSEIGQHHEALAISKIAIKKALKLLFDLHSHSEESLQKKLLEEVLRKSIALPQLKPKQQGIIITFPSSKTQQLTQENLLHIFKDCSTQYIEKANILSYIQMTPLNFDSLLFQVNLTNQQFLHIFSLFVISLYCTSTESRFIEQILDPLQFTEAENYLSRALELVYLYFPKDMPLVNQLLNVHNRFHDISKQIIQEDVHQKHEQNYEIVLLKPILEPQKCGFVIPIIRKSKCSYLIKERAISLKEQQFQITKAQNLNIDTNTYEGSKPQNRKLRSISTTNKNKPNSPKWGLNQVAEIYLKEKNASNHQNINITNFRQTHFKRPQTKASQKQYCFEKPRFNINFLIINNLNHVQLPPKRKRGTSFNLESIKPPQTALNSAKGNKELLKSYQMQTKIKNLIKSYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PHV8</accession>
    <name>A0A8S1PHV8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1180061</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000121">
        <property type="protein sequence ID" value="CAD8102361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PHV8"/>
    <dbReference type="OMA" id="CTHIADI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000104">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000011">
        <property type="entry name" value="Putative ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF117">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="183"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="494"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="272"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8102361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="707" mass="81559" checksum="7DBC439A7D5B7F64" modified="2022-10-12" version="1">MQEKICELLWLQIPGVCRLTQQCVHHMSGMLVEDTPQNAADVQELLGEYFRNGGKLTTQEINKICQKIFDTLGKEQLIKKEKKHTLAAEQLPQEIILSEMDFYLEKESETIKFEDLFKEQVATNTNEQIQKKDRKEKLRQKEDVKAKEAYEKHIKTIKEQKTHIPPARVRHSKADQDGKKLDIVIDKLSIIVGGRALLEDTNLQLIYGQKYGLVGRNGIGKTCLMNALARYEYENADKFRHIQVLLVEQEISETDKNPVELVLETDMERLELLEQKDKLENSEDLNAGLKLQEIYERLELIEAHLAEAKAIKILQGLGFTNDLMFRKTKHLSGGWRMRVSLARALFVQPDVLLLDEPTNHLDLDAVMWLEDYVINCKHTVIVVSHAREFLNVVCNQVIHFYDQKLTPYTGNYDQFEKGRAEKNINQRKQFDSQQKKLSHMQSFIDKFRYNAKRASLVQSRIKAIQKMDLIDEVLEDPSCVFIFPNPEKLRPPMLRIEEGYFEYQLGKPILKGLNFAVEMESRVAIVGANGVGKSTLLNLLTEQRRLTEGNYFRNPRLRISMFTQHHIEQLDLMKSPLEQLMTTFPGASGETYRSHLSSFGLNGNLQLRPQYLLSGGQKSRISFAMAVWNNPQILIMDEPTNHLDIDAVNALIIALNNFTGGLVIVSHDQYFVSTVCDQIWYIKEERLKKFNGDFDDYKRALSEGKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PLW4</accession>
    <name>A0A8S1PLW4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1230154</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000126">
        <property type="protein sequence ID" value="CAD8104380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="KIDEMMN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33487">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33487:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="2704"/>
            <end position="2737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="426"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1423"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1727"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2560"/>
            <end position="2588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2783"/>
            <end position="2819"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="2016"/>
            <end position="2047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2560"/>
            <end position="2586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2783"/>
            <end position="2814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8104380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="3439" mass="401667" checksum="71563C48F0AC6B9E" modified="2022-10-12" version="1">MADKKQQAQAKPGVKFQDQPASVQQVENKPQVIYKNATEQKDQALKQFNKVQTLFKTYTASDPQCIRKDTAQSLKSLLASLRDCMQVLNIGTFEDKNSNYYLIYNGTVYIFDICRVLRKSIYQYIAIEFLAFCITSMEGCIVLTSIKYLDWRIKLYVELAHIYHSLQSKACAYRTIDLGLQKVQELRELEEMDPPLMDYMDKILKQNTRLLKILELKFKVQAGVFTVDQWKKKIEEFGQDKEARCLAIIESLRFSPPNLSNTVKQTHVGNQLKEQLVQVSFDLLKNDMERIAIALEQQMDKITKTVEFKSKNILDELERNDMTKKRREFEASLIKEKEWRALADGLPLEYQIEFAHHAYDVGNIKVFEDISNSAYVRCKYRRIEVPYIQDVNILISTNPYPNIPNGYDKIQIDINEANLRTELKRLRNKNKGDQQQQQQQTQKKDDKKDNKKQPQQQEQEKQQTDQVGLGVQATDSELAQINHNYIYLVYKKSMTPDNAIYEIDVVMADEEQGPVEKMNQGYRAIAIPIKQYTGVREKYKTVPYLLLKHTMTELSDENEKMTLLVGIKPLFGKNPLIRPDFGYQKINLDLRQTPKEFIRSPGMDYIYITQKTDKYYFVKERELQILIHFIKLEKSYQKEISSSIEDDSRLELEICYDLQSLKELMDMISSCLVGPLGIHFLKEKRDFLSQLVFLLWRKYFLPPLYQMQFIQELLITQEMSRQDYSRYDKLIQTAREIFKNGLLVLNEILFKIPYVDIILLCKVNLSLAKFLEEEKEAQVAEENLKICIDRIIQHRNSLTVRGVDSQKDLFLPFAVTCSNFKIDEMMNRMREAWINQKNQINREIRIKNRSNNKKQQLEDDELNEEEYELLEAYQSLLGDDNKDTSTLSIQQKINETDLIINALHADLTVALYRCQLKAGLDIQKNSIKKTSLEEEVEGVSQAIQKKMSIMQGETAQTIKKNVNQLQSTLQKEGKLKPPKPVIHQFETEIMNQVNKNPYANCLLYMLLASIKQKQTEQRSFLVDSLKFLQQAEKEEQLHIENGINDAIFVMSTLCDNIDTQSTSNNVFPYNMLYNPQYIKSTQVPRKPILISRNSESVTFKLPPFKPKLLDMIAIDQAKKTITSMAIFGKISANGVNVSLTCNDLNNTGIRQKIGAIVTMNGLQRNEKYCFAVAAYDGTEGVSNGIGETGDDITTLHPLPLPLLASYLCKVAYQLSNFDICEEAADFCIMQFTEDSEFIDRQLHNELNPIHIKRLLQQRIKSVSLMEIQNLTETLLIKAKCLQKKITNTGTQAQKHIVLLKICNYLLLSLETSLCCRHFTIAKRIVAELYNMLESFQQEKPLQVFHLLLKAQIIIVEIPKQYWDANLRILSAKFTYEILKVCMKLNEITLGRRVITAELQSFNRKWYQVPKIIMIEQVEDTKKDTKKDVKKGGKPQQIDEPAQPPKQVPKLIRELYEVNSTVQDYLEETLLAMNDEFGDYVPSFVEKWKEQIDQLIPYMENPSDQIDKIFSELSIRLEYWECIKDLSTATQKLPKQHPIYIEMLCKLIRRMIQSNIDYKIIQPLAVFNEQFQPSVAIANDITQIQQKCIQLDIEFYPALLPELIIDKNTNKVGNDDAIQLFEEFNTKKTQLLEQLKIEGQTINRFGIMSKAKRFEYVQLWRSELSYLKAILSYKSNRIRLVDVDCFVSVFNLDIEHLKELINENESQKQIKQQQQALQQQKEQQLQQLQQQSQQKQPPAKQPPGKQQVQQPIVELSEEEELIQLSTQIIKNVADACGFAVWSKQYIVMLNHVKFLYNFLLKEQITPFIHLGQCWQDLCFIGNCINMLIREVKDHGWFQVKEQAENELALQPHMPQQDFVFEPPLPNTEPIRPSLLTQLSKDCWFSKYKDMDLLANIMAYIVQSLMVEQKWNSLIGISRQFCNLTTHYYSQYILPFTIHAQTILFKEAQNKTQLKQEELNARTQAFQVWEQTKKKKTRSSLLTQEIPQEEQDYRADREMLIQQIHLLQQKQDFIENQLKVSEKLIQEINRDANQALENLKQARKLYEKFAIDDELLSKESIQLEFNSEQFLLKQTTIDKKQLQLMDQTLKQKKKNHKQFGMQVISKYKLTCELLHKRQEKFAQALALKELGSLNFAMQNYAQAEESWSESLDTIFQRIFVLKSSAFRQIIQECNKNNQLLAQTYGIQQCLIGVTLCSILAYNCYYSNCHQQRESAILASELVSSVLKLQMINGLDWQAFIHSKEVAIGNIFNDRYVLDPAELALSCERCAWLLLDRDEALRAVPLINLLGELSNRLQSNFYSVRAKLLRSIALSSIGYINQAYQALLQIANEKDLPDQSSSLWQSRVSGKWWYSNLEWNNSIPPYDEKHTQLTEKILKELELTREFGLKYGLQNENIFNYAVALLVYNIHSGDIIEKWEINDLRCKFLIKVESILRNTLNKLNQEEQIEQKILTKPKEEILADPKMEDYYHLQGTTKQIGVTNNQEALTYLQKREERMYMMARSRNLMAKVNVSMGQITRALYIFKYAIENLQSYTLELSTNENGEELENLPWDQLKPQGVVEDKKGAKKAPEKKGKEGKESKDKPIADEAPAVKTDAQIKLEETILQIVEARKTRHSINIYYWIKLRTELTLLLYRHNRHDEALEYMEILMNDCKQFNDNFHQRLIIEYQARIQFKKGKSVESIKKFKEAIEIGQKNFHQDPQMIVLYGDLGEIYYEKNELQEAKQQFYDAYQMAEQLMQMINYGYGVVPNWNQKCGQEKIQICQDLCVPLEIEQEILKKTDKQIKTTKKDEKKKDDGKVVRKGADKQTKKDNIDKPQMPIKGLNQLPNFNFIQPTKLTYTTVDIDTINNTQYQYNCYLKNIELWQRASLRYVDIMITLNQANENSTISIDSQESSVASDSESDKSSVKDIFDINVISEIVNKIDTSISKSINVPISFRLELYYLQSKIFKMRFVQLLLNLQQKYFMKYLSGKNKKYADFYEKRPHRDLVRNKYFLLIPPFCQLLKEQGLQYLMQAKESLSKAISVIRGEAVLFEYNRKPEDILVAMAEICLFIREYRVRQGYRYVKVDYLQSLINSKNTEQNKIDLEQQLISMERSDRLEQLNLELEAQSYLKYAIELVKARQELLENFAAVALTPLPDVNKLALEIYTEIIEQDYQYKKKYNPMLFDESKKKVGVSSMDVLQFIQKVWREAKVMTFSGAYLQRMISKTHRFLKLHMQSYQKCLITSLEVAPKNDVNAVFIDEGIIVTKLLYNPVAHEFKVLYVLGGLNKDKIITSLSKEEKDKLIISDEKNVLYGNIYITDANISMLLQDALNLQSKMKESDQQSQKMKERDYKHHKKAFYKLIEQLGFYFFQKPSTIQMQNGQTHETVKKNVAFKKSESFMQEQNEPDILAKYEAIVPELTHDNITILINLFNDQGVLSINHNYLAILRYFHHQKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PR87</accession>
    <name>A0A8S1PR87_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">60S ribosomal protein L8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T1270114</name>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T1510102</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000130">
        <property type="protein sequence ID" value="CAD8105521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000156">
        <property type="protein sequence ID" value="CAD8112474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PR87"/>
    <dbReference type="OMA" id="PLAMITM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000020">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000006">
        <property type="entry name" value="60S ribosomal protein L8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_A">
        <property type="entry name" value="Ribosomal_uL2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023672">
        <property type="entry name" value="Ribosomal_uL2_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_uL2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007180">
        <property type="entry name" value="PRK09612.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="6">
        <location>
            <begin position="11"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="5">
        <location>
            <begin position="97"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8105521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8112474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="29032" checksum="C769D820F7C30326" modified="2022-10-12" version="1">MGKRIRAQRKGKSNSVFKAHQNQRIGSPQYRHLDYAERHGYVRGVITAIRHDPGRGAPLAEVEFKDPYKYSKVTKLFIAPEGSYTGQYIYCGAKAQLATGNVLPIGQIPEGTVVCNLEEHPGDKGALGRATGCYATIIGHSDDGTTTRVRLPSGTRKTLSALCRATVGLIAGGGRTEKPILKAGRQFHKYRRLRKCWPKVRGVAMNPVDHPHGGGNQQHIGHPSTLSRYAPPGQKVGLVAARRSGLLRGGAQLKQMDEDLAAQQAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PRS5</accession>
    <name>A0A8S1PRS5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1280076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PRS5"/>
    <dbReference type="OMA" id="YVEANHP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="875" mass="103457" checksum="B1CFB75A0D72D814" modified="2022-10-12" version="1">MIAQPRYSQKMADTFSNSKDFYSFHQKQLIGTKVHPQLLEHLSISQRTKMATMKDRTSSDIFTSFINRGMLHSEQKELNLQTYDNSSTMMIKQNDDERISKEEQITPQILQTQGEQEKSKKLLPPLKLITNKLKQKERQNQSKERAETFKSSLIETPTMQKTLARNHRAFGQGEQKSTIENETSVHKMGRTSSTPFMQKSKEDKEDHLTSRSGTPQKELFHASPLRHRQKLQNKQAIIQTPQSKGVKEKKLQNAFYSSSSQQQQIKVIDIQNDKKIIPKKKKKQNQGQVIQQQNQQKEKLTQRHTSYRCFINKINFSNNQCICCPSKLEVPSYFYYVGKGNNGSLIKNIFRQRWWWQEVETLDIIKVNMSWTQLKQNVCIAQLHPCIIDSGPQSSESFLLSHEETIGDTSDSDMEARPKQLNSTAGLQKSTPQQQQKRPQNYYSLRRIFNQLDLQKILNYMDETGCQDSQLIFSDCTEKLLKDIKSLCTNQRQDSKNFRMHNHMEDNWHLGNKKALFYNMKHYYQIIREDYNKLLPVTFHVQKGVNDIEYQKFLEYFNRRTEEIKEIERTRDRKDKRKLPINLWIIKPGELTNRGNGITVCNDLNEINKIINEEIQEGRPRTYIVQQYIENPLLYNKRKFDIRCYMLLTSQNGIFKGYWYQEGYIRTSSKEFTTKCLDRFVHLTNDAVQSKDQDYGKHEPGNKISYLEFQRYVEANHPNSKFNFFIDIYPKMKSAALEMMKATYGKIDPHRRINSFELYGLDFMIDDNFKLWLIEANTNPCLELSCPLLSRIIPTMVENLFRIAVDPIFPPPFFEEWPQNKKLFIPDNVVENNKFELIFDELIEKKNMINLFRDNKIEQECFDIEEEEEEEEEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PT91</accession>
    <name>A0A8S1PT91_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1280114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000131">
        <property type="protein sequence ID" value="CAD8105798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PT91"/>
    <dbReference type="OMA" id="FYWKVLT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="1">
        <location>
            <begin position="244"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="328" mass="38302" checksum="5C603EB742F2AECA" modified="2022-10-12" version="1">MFPSKLYDLQLTPSLNKFNSDFQSARMIEQFKQLLIEDDKEILTSALDLMKAISYSLYGTWFYWKVLTDICKGDDLNIISQQLERKIIIYDICEIEDLKCQVINYGYKKPIYLARYLNYFYVVGKKGTSEKHKLIKDILMDVINEIIGLPIRRHKRSYSDAFKYLSDQKDNRTNKLNIKNVPLSPSNISRTSLQMQLLDEVNQESDLLLKECNSLINSSSPQNDLLQSLNLNLQEGTNILSNIRYFGTLKFYDEARAYGFIIMDMDGSDLFVHCDDLTKAGMNKDFLRTAKHGNIIRFSFLILEYFGKYNKSRKAVDLQFIQGQQYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1I0</accession>
    <name>A0A8S1Q1I0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1410033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1I0"/>
    <dbReference type="OMA" id="LEFDSCK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035937796" description="Cystatin domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14745" checksum="80EDE7D3A0503F61" modified="2022-10-12" version="1">MAKLLLVILVLSLHVSLNESISIPSLNHQVQMNQKPKKKPAIEWRNTSEFENNPTYVKVVEVAKNNFQSQCNMNKTITFSKVLQVAKKIEQGFLWSLQVEFSDKSIKIIQIFEDLDGNLEFDSCKDNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q229</accession>
    <name>A0A8S1Q229_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1400025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000144">
        <property type="protein sequence ID" value="CAD8109202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q229"/>
    <dbReference type="OMA" id="RKQDEYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="47105" checksum="4ECCE59652A5FDC3" modified="2022-10-12" version="1">MGGQISKDDSNDHLVNISEIIKKEAENIEPDIIIDQFTLTQKKEQIKAQKNKSQIKLGEYTTSQIYPNNQSKKLLIEDLKSEKSQEFVEHQQVQMPSGTINVSQIKLNDQQNQQNILSNNQSRIFKKNNPTKVNDPDFQIQQHLPEWANKMIQEYGYYQCPEEINYQNTQEAILKYEDGSYYKGQIQGDYRHGYGIFLQEYCIFEGYFEKNIRNGWGRELQKDILKQGIWRDDQIIEKLEIRKQDEYYIGECQYGIPHGKGKLENKEYIYEGQFKNGLKNGKGQLKYKDKKDYYDGQFKNDKMDGNGKYYFSNKVIYDGNFKDGQITGRGEMRYPNNKFYRGDFLNGKMHGQGILQTESGGKHEGQFKDGLKHGYGRTIQNGMEVTGEWKYDVLLEEDLDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2H5</accession>
    <name>A0A8S1Q2H5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1410150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2H5"/>
    <dbReference type="OMA" id="NLRQAIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="114" mass="13072" checksum="8C3CD275100600E9" modified="2022-10-12" version="1">MIARLSTTFLKNMRQAICIVPDTEITLNDKVFYFEQNKVQSIPIKQLLKEYLLLESEQIEDASKVEQDSKENLEQQEEYSDDENNGDDQGPSGGRMMPVIELIKGFIPRYVGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q3P8</accession>
    <name>A0A8S1Q3P8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPRIM_AZ9-3.1.T1410151</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q3P8"/>
    <dbReference type="OMA" id="DRCECLG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000537">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000048">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="100"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8109764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="425" mass="49312" checksum="3836F68BDC17F868" modified="2022-10-12" version="1">MGNMCYRDYDEEKLLGGQEIKKPESVQGSIMKPKPSGDGQTSEVVNQNQTEMTDDPELQVEKDEFYKLSNENTRQLDIASKAQESEGQGQQTQKVNIDSFRLLKVLGKGSFGKVMLVQYKSNSKYYAMKVLQKKNIKNERQKRHTETERIILATCSSIFLVRLRYAFQSQYKLYLVVDYMPGGELFYHLRKVGRFKEDVAKFYAAEILLGLQYLHENHIIYRDLKPENILLDENGHIKLTDFGLSKIMMEEDETAFSLCGTPEYLAPEILTTKTGYDKTCDWWSFGALLYEMLVGAPPHYKENKKEMIRRILTQPIPYPSYLSENARSLLEQLLVVDPKKRLGYENDGYDIMKHDFFIGIDMAEIIQHRIQPPYKFDKKELKYFDEGMTKQIAKDTPVNGTLLPNQNFSNFTYQPSMQQGANMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q3X1</accession>
    <name>A0A8S1Q3X1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Calcium uniporter protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T1420085</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCU (TC 1.A.77) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000146">
        <property type="protein sequence ID" value="CAD8109973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q3X1"/>
    <dbReference type="OMA" id="MEPITCM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015292">
        <property type="term" value="F:uniporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006769">
        <property type="entry name" value="MCU_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039055">
        <property type="entry name" value="MCU_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462:SF10">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04678">
        <property type="entry name" value="MCU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Calcium uniporter protein C-terminal" evidence="15">
        <location>
            <begin position="151"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8109973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="362" mass="42901" checksum="2A80989326D2E2C1" modified="2022-10-12" version="1">MLLKSLFRFCSLKRYNFQDILMLQRFSQEESKNLNLKQEVLYFYFHSKKVFFKGEGTVLELQQAIKKQIDDVKSVGVYQQTNNDEQVEYASSTPMQLVLAEDMVLRLNQTENYIILNKVDNPPVIDLIDNYPITQHALHEYFQEIDVPQRTKVVLSNFIQKLLSEIEKHKELTYDKDLIMLIIKKVIMKLGIYSQAQKQIIDDQISFLESKQNRLTHLKDSIIKELEMKEQTTLYQIYFYILMQIAFTQYGTYVVWGWDVMEPVTFMLGVTDMIIAYQFWMKTTKAYTFGNVLQNAVNKHLNKRLAEQFNYSDELNDVNRMIEYLKFKQMVYSSDIYEVISVIEGAIEEKQAKLQAQEEEDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q4U7</accession>
    <name>A0A8S1Q4U7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1420145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000146">
        <property type="protein sequence ID" value="CAD8110055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q4U7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="727" mass="85018" checksum="4CACFA39A388B1BE" modified="2022-10-12" version="1">MSYCIDHPQQKISAICVSSHKCQRKLCDQCMSNHLMTQQKPLPINIFLDKLQEKVNQYKLKENQQKQQFRVAFKCVLDSTEQMIIKLYKQLDESIEKIFSKMEEQDQSYINLLDNNSNPTDSSINDLEQLVQILEGNTLEDWNNQKNYYMLQLETSKNWLEQEMKNFSKNFNFEMSEILSNFKYIEKSFLWNYSKERFINTEYQKIFTKQKEIQYIREGSILRSDIIIDSSQKPQMFSNLEQIQYLNWIGAYGKYNQKVGKWLATWKGEPINKVGGQYSDYGRKLGKWKELINNYWSKAQAYEIGEYENGQRRGAWKCIYGDKEIGQGDYNSQGRKEGKWIELTDGFWDQSQAIFQGEYRDGKKVGQWDIKLRSEGQEEFELIGGGSYNESGDEVKIGKWIELPDGFNNEQEVTLIGEYEKGNKFGRWDILYKGDQIGGGSYDEGGQGIKIGEWIELNDGYDNWKQISYSGQYKNGKKVGRWDTYWNWDGENKQIGGGLYDEERVGIKIGRWVELSQGFRNNSQVTYNGEYKNGNKIGRWDIMWKQEDENEFKLIGGGLYDEVEDGTKIGNWTELSEGFWAESQVAYVGEYKKGIKIGRWDILYHGKQIGGGLFDEEGNGLKIGKWVDISEGYQYASNIIYIGEYKNGKKVGRWDVFDEDEQIGGGSYDEKGEGMKFGKWIEPWEKFDWYNSIVYNGEYLNGKKVGLWVQQGKSNGEEIYKEIRYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QA71</accession>
    <name>A0A8S1QA71_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1520067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QA71"/>
    <dbReference type="OMA" id="IYILKFR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="296"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="331"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="397"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8112639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="552" mass="63386" checksum="4368FCE2D1E1E031" modified="2022-10-12" version="1">MKKHPIEQIHSSVYDKVVKSLTLFFMIISLLISIGLDIVHGVVDFYSNEYQYKAQNATIGYISDNLIYKPIKQVYFRDLDCDTSEEIQQLFPFAEYNITRWFSQLICVTRFQDIRLSTECTETEYNCGPYCIQTSEGSQCPISKLTIIQNEIVDEDADQNLESNRDLYIQRENSSYGIFKIQVVIRGYPCLNPLIYITAGLTNNSDYIDPENCEGSDFDTDNSVKIEQTTLSEFSKQGLEALSFNGVPSSVIGSAITYLTARNKIASKAEDSCTQIDNNMINETENCEQSIQEIGLIYVVIKQIFTGLIGIILIIEIYILKFRIVNDIRIITVILKILLVCQMSFILIEGGLILYQEKLRIDAELYFKSIANCYISDQIYNVFANFPNLFPADVNTYVSLITISIIIIAISEFILVMMFIVQMFHWCFKQKKEKLYVSEPDTQRQQIPSKEDIHHSETSPYQKPLSPYQSKFPPIYSNIQNNIIQNQSNPINQESKYVNEYVPPNQFEDPKYIPPNQFGESSQPKFSESYIPPQINNIQSTLVVVGKKNSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QA85</accession>
    <name>A0A8S1QA85_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit TIM16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1520077</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QA85"/>
    <dbReference type="OMA" id="AKYLIQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005341">
        <property type="entry name" value="Tim16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388">
        <property type="entry name" value="MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03656">
        <property type="entry name" value="Pam16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8112659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="18020" checksum="5648FB7EC1B2A3FC" modified="2022-10-12" version="1">MVKRLIIKLIIEVGSTVAKSFMKAYQQTAKQSGGKAGNPFTEFLNQTMQAANLTHKPMTREEAFKILQLVPEKASPEDIIRVYWRQFHKNDPVKGGSFYLQSMLHNAKCELMKDFADVNEKEILEKIKKEGESQKGDEAEQKDEAKVEEKNEEKKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QB41</accession>
    <name>A0A8S1QB41_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1530091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000158">
        <property type="protein sequence ID" value="CAD8112959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QB41"/>
    <dbReference type="OMA" id="SHEHHIL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="133" mass="15913" checksum="CE37F89B72F0F860" modified="2022-10-12" version="1">MNQKVKIECVKQGYQISNEMKFDIMDFYNKSLHQEHYAHKTDYIRHNLEEKYGRCFSIILYQIGAFVSHSFLHADDFLLELRSHEHHILAYMLPPSFNPPPLIPEPKVIPFSRTTTQPLHVTKQFHNNGYYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJ11</accession>
    <name>A0A8S1QJ11_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1600008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJ11"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8114435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="39118" checksum="F4F1B2D816CBE52A" modified="2022-10-12" version="1">MKNQSLKYSCKIFLVILILLQKTTIQQLLFYSNLQILKYKKPIPLFSSQDQIYRIELQNFPIILNQFEKLKLNQSVIEIIFNCFSKNVHKIMLFLEVVVQMKILSIILHLDLRNSCIQKYIQVNQIQSQKNLNKHYIHQLYCLKFLDKQLIKFQIMVVIFLEQRIIAVIEEVSYPKLGQPLAQIGRIIKLIFLFKHIITIYYQKIYLQILLSRCVAQNLKSINLTFYIQIQILNKLINIIQILSYPNQMQQALLEEAKQNYQLIYVLQEISRIQFKLQEKFGEQILILNSQIIPNFFTKEIEYDNQTKSNRFQNKSFDPLEIENIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QJE8</accession>
    <name>A0A8S1QJE8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1600069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000165">
        <property type="protein sequence ID" value="CAD8114555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QJE8"/>
    <dbReference type="OMA" id="WNIPKEN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8114555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="561" mass="67889" checksum="F63B25C886C22E8D" modified="2022-10-12" version="1">MFNYNIDDYSAYMISHDQISLTYRGQITKEIYQFKFLQFDILEEILFLKQEETAVSLPWAKTIIVNFWNIPKENYTLFNLYGGMKILFDMDIIKDLQEVQFKFFSNEDIQALFKLKWLQSDNSNYFYFEIQEVNQYELTLQWRDPIQNAECSSLELERQLLKRNLTQLLNKMGDYNNIYDQQELFEMILENQYDTIYIYFPSIIKLKMHTKIFEESEEQFRSVELFIHNEWFKERQDNLIAQFEILHSQIQRSMNDYPDEIILDYALIYDEEQINCSQLMSKINSLIIKILLTNNKKIQTILLSLMPLEIHFELDIKRQELNLDINLHHEDPQDIYQEGLIILLQTITTCKLFQSLKVDIQQEDDSNNVKFLLFSFIMMIHEFQQTLIKKGTFTYNNYSLIPRYNSVCSKGAMNYLISEKKSIYQISEKDYLDIQREIGCFIQYLNWYAKDNNSIIISCDMCELSNSSKQFYDQLIQQLIKQNTIFEFGILDNVNRDQKYYERVTQQLLLPLIKYFRQKKRILTTMFMIDRKYQSRMRWETIKEMINYLPNEYQCLLHLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QPT4</accession>
    <name>A0A8S1QPT4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1720001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000181">
        <property type="protein sequence ID" value="CAD8116410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QPT4"/>
    <dbReference type="OMA" id="RTENDQF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="303"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8116410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="530" mass="62607" checksum="4B0665B008E78876" modified="2022-10-12" version="1">MFKPKMIENEKDFKCSKGHKLQIVTIALDPKLSRNQRLLCKECLENIELDAKVVGLRTIISLIEENQGKKLEKIQNVIMNEINLIEQLHGIVDQMKSFVIQQFTELITIIMEWIQSLKQQGSQFTQYSFYEELEIMILKQNKTDDNLPILINEIQKTNLCWISKFNPKLDYFNQLLEYNKCQEILSNVNLGSQNNTQNKQQQQFRGGEIKLKLIDQSVKQSERCDGIAFDSSGSIMLSTQQNDIKVWSFQNGTIKLIKTLQGHTKWIQCLIYSKKQNSFISGDVTIRCWKQQDSTKWISSQPYEEHKLFVKCLLLNSNEDLLFSGSDDESIKVWKVDFNKNILKYIYSLNKHEGYVLALSLNYSETQLVSCAKGKNQIIIWERKEQDKYEFKYFVKQSIQEQGKKVRFIKENQFIWVTSCKEIDKFYVFELKEGVFQEIQEKTIDLVTNNQILDECRFPIMYNKDKNLIIIRHKTYFYIIREMNNGNFKIVDQFNCDSYSIYGNMTNNGQYLVYWDDKKKGYSIYELSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQ80</accession>
    <name>A0A8S1QQ80_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T2120004</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000221">
        <property type="protein sequence ID" value="CAD8117816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQ80"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="303"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="345"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="357"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="387"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="429"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="471"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="513"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8117816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="630" mass="70947" checksum="E2E9ACC055D0C677" modified="2022-10-12" version="1">MHCTFEQKDAIFNLFAQVNDVDEQIFSFILKIFKKEKVFDIIQYLSDQENKRHLEQQVLQQQENLTQVDYQKKLQVASSNIQKITNFINKVKDHDFNNKDYSKEENQESIKDLIKRIKDDKQFIEFLQFLVHLTTLDKTLIQCGSNSLHLLVQMKVDLRNKNLENIKIENTSLVGANFVRCNLSGSEFNNVNISGINLNGALLFNCKWNNLKILEFNNLDGHDGYVSSVCISPDGSTLASSGYDKFIRLWDIKTGQLKATLDGHANPVWSICFSPDGSALASSGYDKFIILWDIKTGQQKAKLDGHANCVYSVCFSPDGNRLASGSSDKSIRLWDVKTGQQKAKFDGHNSQVYSVCFSPDGTTLVSGGYDKFIRQWDLKTGLQKAQLNGHTSPVYSVCFSPDGSTLASGSDDKSIRLWDVKTGQQKVNLTGHNDTVRQVCFSPDGTKLASCSYDKSIRLWDIKTGQQQGKLDGHTFYVYSVFFSPDGTVLASGSDDMTIRLWDINTGQQKDKLDGHQYYYNSVCFSSDGTILATGSKDDSLRLWDVKTAKEIQATDKIYKNIVAQFKTPLFQQNPFQVSSYSILVISKQINLQAQGTQILKGEFINHQGKDLRPLFLSKGSCILEGLMQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QQP1</accession>
    <name>A0A8S1QQP1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2220004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000231">
        <property type="protein sequence ID" value="CAD8118058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QQP1"/>
    <dbReference type="OMA" id="LIQWRIK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8118058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="30216" checksum="9E52B59CEC5008F9" modified="2022-10-12" version="1">MYVFAIPGLTIFGLLIPLFFFFLLLNKKGKLAIIKVRRHICYLFNEYNQESFFWEQIKFLKKTSIILVLTFFESNILFKASLLGLCLLVYQLLAGKQQPYIISSLNNLDLQTGQICSIAIFIATAKYVNEQEAENVSSQILQIVIIILFIKLCSLFIFDIFRGYAKKYKVQLFTLIHNIIKMIKPNSQQAKYINNVITQLKLKEIRLRSNYQILKTASQEQIKRQKCNNQAIVNQNLPSLNRYKKYDSATNQFLLTIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QTA9</accession>
    <name>A0A8S1QTA9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T2090010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000218">
        <property type="protein sequence ID" value="CAD8117770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="513"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="555"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8117770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="629" mass="73144" checksum="13FABAD65B2297DA" modified="2022-10-12" version="1">MNCTYHIKSPISLICIAPHKCQRKLCVECQYEHGVDLKQTVPINKFQDMAMKKLKDSKLDDNQELTKQRLALKSMLSQIESTMKKIWEELSESIKQVYNLIEQQNKAYIDLINENTNLTEISNTELEKLIQILEGKQLNVWNAQKNFYLNQLDKVKNLWGLQIKSFNEKIKQETNEIFQQQFNPIQEESEVYQSKQDLYEMLIYIQDIDESLYQKILDILRKEQVSDTIGFLSKTKNNQFYESLNYKQENIKEIKKQVKKIINVLTNLQDHQYNKDNYSSKTYEQARQDIIKRIKNNKGITNFIKFLVLLTSIDDKFIQSGSNGLNLLVGMKVDIRNLSLENIRIKNTSLIGGNFVRCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXITLSVYGMSKQDNKKPNQMVILVMSGQSVSLLMEIHQLLVVMISLSVYGISRQDNKKPNYMVMRNGQCQSVSLLMEIHLLLVVQITLSVSGILMMEKKFHSKTKNIKIFQLNLMHHYFQIILCKNLNILHFYEYLKHHYFKHKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JM01</accession>
    <name>A0A8S1JM01_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0040385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8043162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JM01"/>
    <dbReference type="OMA" id="FIGSQQI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="329" mass="38316" checksum="019038358D713698" modified="2022-10-12" version="1">MSIRQESDLSCELLRSTLQDINNRLNNLHQCVPNPHCRNDKMHKEAPIPRLNLHKINQVQKENYLPLKKVTASFGDQINDQIEKIDENNFDDLQKKPQIKMMAQQIPQKLRCHSINNSQQILKNQNNHSIQFKVSNASRLKNSCDIQYFQSLHQSHEKPFQSSYICGQYSQIAQKDESFEYVKPTLMQKPIILEQQQQNVQTRNTSQLSTPIKQVANVSQGQTPLKRGNSSTNKKMIQTNLNQIQSYQCFQVQQQTQQLYNQDLIKILQNHSNQYQQQQIQYHSVQNALNSCQNTQNMAQFKVSQNRNASNNQRVSMGQMLYGNSMVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JNU3</accession>
    <name>A0A8S1JNU3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0050168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JNU3"/>
    <dbReference type="OMA" id="MKKNCKT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8043515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="127" mass="15172" checksum="5C508C24749EA930" modified="2022-10-12" version="1">MKKNCKTKLYSFQITITKKGFLNFKNQYQDQKSKVLEQNQSIKYNQQVNDFTKNQKKNNNNQSEQMKKNTICVSKFDNKLLNSLNQLENLLDKSEKEETPLIFANEDQIKFKYFENNKDDNDKKLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JPB2</accession>
    <name>A0A8S1JPB2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0060384</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JPB2"/>
    <dbReference type="OMA" id="DKLMPNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010402">
        <property type="entry name" value="CCT_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06203">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCT" evidence="3">
        <location>
            <begin position="20"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8044302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="124" mass="15022" checksum="3748A2F883A5F203" modified="2022-10-12" version="1">MKQLKIIQTNEEIFAKQIIRRQSIERYKQKKRFGNTFQIYQIRKVIAERRLRINGQFLTQSESEKVLLARNILGKNKVQIKNNNVKIKISNERQKENLIKFIDKLMPNQILQKIQNKQRIFKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQ29</accession>
    <name>A0A8S1JQ29_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine carboxypeptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0050562</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF113">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CTSA-4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8043907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="23557" checksum="7B210B13CABA590D" modified="2022-10-12" version="1">MLFYLFICFTHAAITKKSLKDLYSKYLTIDFDVESGSASGLSKEKNNMFYQLVYQKGLNEQTITKDHTFLIWLQGGPGCSSQSAFFELIGPFRITKSDTDFTIEKKDNSWNDFASILFIDQPFETGFSQGEILINSTLQASTXFYIINIMRWSKQLIFIRTKHQSLKQTFSRTNGDFYLEFHYFFFRNCIQILCKQLYKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTR9</accession>
    <name>A0A8S1JTR9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">cGMP-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0100185</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8046104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTR9"/>
    <dbReference type="OMA" id="HVINERK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05572">
        <property type="entry name" value="STKc_cGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000154">
        <property type="entry name" value="cGMP-dependent protein kinase 1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000123">
        <property type="entry name" value="cGMP-dependent protein kinase 14-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000089">
        <property type="entry name" value="cGMP-dependent protein kinase 5-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035014">
        <property type="entry name" value="STKc_cGK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="109"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="227"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="348"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="481"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8046104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="807" mass="92566" checksum="1426DA8D3E86794C" modified="2022-10-12" version="1">MGNICRQNPINDKNDLEVTSSQPPPIQQMPPSKPEEVKTQAVEKQEEPAQSIDQAKQENEPKRKEQKKMAKIAAVIDQEVIYENVQKQEKVKSPFDYQLLLNAFGNSFIFGQLQPEDKVKVVDKMFYCTVHDGEMVFKQGDKASSYFLIERGQCQIIINNEVKKTLKSGEAFGELALLYNAPRSASVKAIGDCAFWAIDRNTVRKAIESISQKDYDQNKEFINKVQFFESLTDDQKAAIPSALINLNFKAGEIIVNEGDQADSFFIIKKGEIQISRGGKELRIMKAGDSLGEQALQQNSVRGATAKAIKEDVTVLALARDDLTRILGDKIQLIMYSNLQRWAFERHAVLNKLTKLQVERIVSNMQQTQKKSEEVIIEKGQPCREIIIVLQGSIKYGKEVLEKGQMFGDKFLDQGENVKLGEPVVMKDEGMIAIITFKQFFEIIGGSLEQIFAKNEKAHDRFIKKEEGQKQDVFKHFQLDQLIYVKKLGQGQFGNVYLVHNKIDKKIYALKCISKAQIIEQNLEKHLAQEKIALETVNFPLIMHFTRSFKDNTYIYFLEEYIRGMELFDVIRDIGLLNTYDSQFYIGSLILCMEYLHLNNIIYRDIKPENIMIDEKGFMKLIDLGTAKNLKSKNGRTYTIIGTPHYMAPEILTGKGYTYSVDLWSIGVCLYEFMCGNVPYAEDADDPYEIYEEIQKKQLTFPSVLKDRKAKKLIEQLLSKTPELRLGSSYASLKNNSFFERFDYDLLINRELKPPYLPPKNKLHSDKDIQKAIQVGKLITEEIKNDLATAQNVYKPEKARDPNWDKDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JVA9</accession>
    <name>A0A8S1JVA9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0060440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JVA9"/>
    <dbReference type="OMA" id="WEIKFIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8044356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="128" mass="15290" checksum="826B77D1EAF9C854" modified="2022-10-12" version="1">MGTKRKRVIMQAGSLLNPIFRYVLKMNFVSNPTDVHGTKLLRHLQYQTMNLDKKLHQPNFESTYRESVECISSKWEIKFINGWERTFYMKYADMSTLYQTIRQYNHFIEGQRALQGEDDEPDDALLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JW46</accession>
    <name>A0A8S1JW46_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0100002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JW46"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="44" mass="5305" checksum="D84F94D4FD0B1DEE" modified="2022-10-12" version="1">MKLSNSRGQERKKSHIKEKRKKLTRMSTRKVDINTQQKSQLLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JXA0</accession>
    <name>A0A8S1JXA0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JXA0"/>
    <dbReference type="OMA" id="IMTLMYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="310" mass="35345" checksum="AA455735A134B9D6" modified="2022-10-12" version="1">MDDRMSEKLSKVESNLSFHANINNTNLLNETQELIPTASKLPPQALCGVYVLCQLLQLMIGYSLVQNIDAITIATQRCIVNIIMTLMYIQFFDKRLFQLDQSCFRVIGAMAGCQVVASLVGFWAMFYFPFSEICAFHQLTPGYIQVFNHFLYQAGNQLNLVISLMQTLSTLLFGQNLLYVFVCLLQPVCLAFSHVIKFLMNKQLPFFTLMLYQSLFGLPVYILLNLMAQEVQNIFSGAYFLEITFMTILQMIAYVAQNEIQAQKTIKMWILPGAVTILSLFVQLGYHQVDLNYGTLSIIFIVFTSINNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JZQ8</accession>
    <name>A0A8S1JZQ8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine(9)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.221</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0120066</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.221" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8047757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JZQ8"/>
    <dbReference type="OMA" id="FKKNDGW"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18089">
        <property type="entry name" value="SPOUT_Trm10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF13">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="4">
        <location>
            <begin position="67"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8047757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="252" mass="29893" checksum="3618D5D9345314C2" modified="2022-10-12" version="1">MDQQQGENKLSKKQQKKLAKKEKQREYYKEKKIQKKQIRKQQKQEQKSNIVIDDDNKIHNQERQHKKPREQYQEEMKNGIKIIIDLQFMSQMTIHEKTSLYKQIELCHSNNFKSKKPLNLIVTSLTEDFKQLLNKSNASNWGIELNEKPYIDLFKKEDLVYLTGDTEDVIDELNQEEVYIIGGLVDHNRLKLITYNKAIEQGIKTKKLPINLNLKTSSILTVNQVFEILLRRNNGEDWNDSVQNTIPKRKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K1J6</accession>
    <name>A0A8S1K1J6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CFAP184 CAP-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0120392</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K1J6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056294">
        <property type="entry name" value="CAP-like_CFAP184"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23448">
        <property type="entry name" value="CAP-like_CFAP184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CFAP184 CAP-like" evidence="2">
        <location>
            <begin position="108"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8048267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="241" mass="28601" checksum="EB949919DB85044E" modified="2022-10-12" version="1">MSEQDHDEYYDEQEGDQEGDMEGDDQEIDFENMTPEQQQQFLAMHGQYYQGEEEDEEEVGDEEVDDQEQGQRQMNQGMGSSSQFQQDNAEESRHSPGRREQNDEYDPDEILRKVKRDLLENVNRIRREKKLNPLYIELMPAIVAERYASHLLDNDHSDAEFKKLIEFYKYNEKKLDDLKLIYIVSKFEEDTIPDAQVIYDYFMEMGYLFFEVEDDRKILLSNEMNHISIGVSCDDNQVAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K2Y4</accession>
    <name>A0A8S1K2Y4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0080224</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K2Y4"/>
    <dbReference type="OMA" id="SEYTGHL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="79"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="497"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="509"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8044658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="520" mass="60468" checksum="A62838057E453413" modified="2022-10-12" version="1">MDKKGNFERLRNKLKSDLFKNENQQKQNVQEIINQCPQILEYVHCSSQSEFKEDAPKIIDYLSNQKQLEQMKQSFKNIYQIGKILGEGAHAVVRQCWQITNPDETYAVKISRNADPEITEIMKQTFLNTVSLNHPYICKTNMLYIDPNMECSYLVMEYLPYPSLQHILKERKTLDLNETCIIMRQLFEAASYIHKVGLCHRDIKPDNIVFDNDTNTIKLIDFGVSKRFLVAEKNCKDIKNNLMWTVTGTMPYQAPELWVGSGYSKQIDVWAIGVVCYQMLCGKLPIDQENQMESFSTQSEYTGHLQQPEFLQLPPLVIDFIKRILKWNPEQRITSAQALLHPWLYQPKIFSKHIKMRSKDDIVLGYSDKPLLGIVRSMQANMKILQQSVSNSNEQFIEKRDLTKNHGQIIIAYKETSQQIREKSIHFQQSIHSINLTKCSAKSSTDDVKDLFDVINFCDSNSSFESLEEKQQLQAQQQKQAQQQQQQKLGFSQRLGVIKEEPETPYSDQLKKTTKEIDQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K312</accession>
    <name>A0A8S1K312_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPRIM_AZ9-3.1.T0120317</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K312"/>
    <dbReference type="OMA" id="AYRINCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000771">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="10"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8048093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="480" mass="56775" checksum="D7E7D4D2C6A3C04B" modified="2022-10-12" version="1">MDKLIGHYRFNMNHLIGRGAYGSVFKGKTQQGQPVAVKVIDKRMINKENEKHLKNEVESMRTLNHPNIVKFLDYYETQNNIYIISEFCDGGDLRDLIKRGKQDDNLVISILQQILNGYRELQNKSIIHRDLKPANILIHQNVIKIADFGFSKRINFENDLMNSVAGTPLYMAPQTVLRQPYCSKCDIWSLGMILYELIFQKPPIKAQNVLELQEQIKLPIIVPKIDNQLLQDLIQKCLQVNEDERISWDEIYNHELFCQNDYYMQQIQNIEEEKGQQLSESQSISNQNLLEYQRLMIKNYLEIEFENNQKLYLNIINTTRSLYNFSIFVNAQQRQYQYAQQLAQYLQGCAFFHLNLIDQSILFKEFDKFILTESGQYCLQQLQFLKQMIQEKNIQLLNSTFNQFDLNELQKFLEYAIIQITTQSAQMNHAKLCFILMSKYIIEMIKYENEYPNKHLFQLIDIAQIDILSDQELAYRINCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K321</accession>
    <name>A0A8S1K321_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0120322</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K321"/>
    <dbReference type="OMA" id="RMERNND"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000184">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="188"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8048105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="386" mass="45494" checksum="57C0F0D67D65C66D" modified="2022-10-12" version="1">MSRHQNFQQSNQNGFMANNIQQPLPVNQPSYRQNFIQCRGQTQEKKTSEQPVKQMSQPQSNLTETKPSPIIKIAEKSQITTPNNFGKKKNHLQLQKVVTRMEKNNDDSTFNKIDNHQKIIQKIQSNRYYIDDIITAFQDETFNFSKLFRDNFANSFNFIPTLKQVQFQQVEDKEIQEKKIKLEPYPQNLKFKKTVIFDLDETLVHCNEEETMSSQIVLPITFPTGEKVNAGINIRPFAQKMIKLLSDVCEVMIFTASHECYANEVINYLDSEKRVKRRIFRDSCVTDENSHYYIKNLEVIDRDQKDIVIVDNASYSFVHHLDNGIPIISFYDDKLDNQLIKLYRFLINKVLPQDDVRPFLKEYFKLDQIDQFQTIIEAVEKMYCIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K6I2</accession>
    <name>A0A8S1K6I2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0100122</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000007">
        <property type="protein sequence ID" value="CAD8045978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8045978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="270" mass="31879" checksum="330DD4CF3D14E3B2" modified="2022-10-12" version="1">MKTSYLILILHQNQEYCCINIFQIYLKQHTHNESXIHNYLLQKFPNKQDILLSILDIKIQSVQELLFNSFFINYFGESNFFVFLNAQPYEKLNFISSNTQLNLLETFNTIRSSMMDSSDESDDFQQNNQFNFCHSQFEDSPMLSPKIKPKHQSRLSSRQVVLETLLNEQIITPQQFQQFYKEEDFQNEQEINEFSEMLSKEIENSQLTTPIQSDSTATCPNTVIIHSRKNVVNRISDIILEAQQEFIQATKKPYNVNKVQNVRKNLFNLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K774</accession>
    <name>A0A8S1K774_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0170172</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K774"/>
    <dbReference type="OMA" id="NNDSQIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="102"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8050667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="405" mass="47438" checksum="80F61696F6314FAE" modified="2022-10-12" version="1">MQKRQSHNKIPSEVVQSNNYYFMPKSLRSSQTSADNLEIKKVQSTTNVDQFEPLYNPEKLLMNTHQQNKVQQSNQELQQALHYSEQNNQYLSRYHQQLLQNNVDLQSLYNKSEMENKKLLNELKLLQNRISLRENELKQAQENGVEYIKDIAQLKIQIQKLNSTLNKLTEENEQFKEQLNVTGTQTLLLEKGLSSRRDLNQQNEFNFSESRQSENSRQQQSQKDSNIQINSVITHDNISNYQNQILQYQKELTEKNLLIASLQQQVKNLTEFQRQLMTKSPKKLQNKGIKSQIQSSPQQTPIQTLRNNNDSQIMSNKNLLQLIDEQYKPNFQLHRTRQSDEFDVAPSIKSSITNSKSKQELQQIYKKINQSTIYTAGLYSSLLDYQYLSNIKLLNSSCTKDTLQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K7L3</accession>
    <name>A0A8S1K7L3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0140163</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8049785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K7L3"/>
    <dbReference type="OMA" id="YWRIIRE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050776">
        <property type="entry name" value="Ank_Repeat/CDKN_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201:SF15">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="97"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8049785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="18003" checksum="B2414E18843E2F83" modified="2022-10-12" version="1">MLQTYINRFLIKLGIIRKDESFIVREEIRENATWSDLRQNRIKQIKDDLESGNINVDQEIIEQSGQTLLTQSIVIDRYEIFLLCLLYQANVNQQTYEGMRPIHLAASLGRLKMIQDLKLYGAELDAKDELGMTALDRAKLYNHSDIIEFLQQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K8B1</accession>
    <name>A0A8S1K8B1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0170335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K8B1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8050991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="14115" checksum="4A30FEE51E1F3670" modified="2022-10-12" version="1">MHVLLHQSSDKFQMGSITLFMLQLCGLVFLYVCQKELLEQHLELQMLFRILDQQYFQLLQAIQYQMKLHRHMLLFQAAVLLLDQLVILDYGLLINKMGVKQPRLVSKIILKFKIQIATGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB56</accession>
    <name>A0A8S1KB56_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0180315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000014">
        <property type="protein sequence ID" value="CAD8051753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB56"/>
    <dbReference type="OMA" id="CECDMFS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="83"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8051753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="444" mass="51653" checksum="95D89332A9F863B7" modified="2022-10-12" version="1">MNLDFICLRKHLFKDTLYQIDIQNEQLRLINTTSGKPKYIIRLDWSLEITWNITSQQQAFGLKVNQKFKWFHAKPQIIQNLKKQLQKYVTSTDFNMFYTLTTHQSKEQNNEYEVLKKSDNTANLVARCIQKDQIDDQNIIYGELNILKLLNHQGLPVFEEFFFTSGTYYIIMEKVQGEKLSTIVNTSKLQLNLRLIQSIIIECLQILQYLESINVMHRDITPDNLLYEQQNKKKGVKLINFSNAAKLGTEPKKCGTPGYIAPEIFLDQQYGCECDMFSLGCVFYKLLAKKDLFQGTSVVDVLSENKKCVINLKTLQLIRIPQIAQDLLNQMLETNPKLRISVQDALKHPFINDPLKPNASNQNSQKNFNQNRSERYLIKKELQSLHSQKQKLDEDLDVDYFNNELPPKCEIPVMNSVKGGQRFRSDCIEEIDTKFRRVVSKSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KB79</accession>
    <name>A0A8S1KB79_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0170291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000012">
        <property type="protein sequence ID" value="CAD8050905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KB79"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8050905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="257" mass="29841" checksum="B2C073BF6B5B47CA" modified="2022-10-12" version="1">MELKGAFSYLIQILSNYILDNSILLKLTQLRQLLDNKQTKELEIKKFIVSIGQISLLSNQSKETKNSDNLILISKQKYLSKQQSRFLSLINILQPLNQNQSPINSKIQRQTNTHRSLSNNSNDVHQLNDRLITQITDRVKNQKRQFNIPNVFIKTYEDNSSNNSPIQSPINIYNSISNFGSIKNIKSELQKTSIFQIKTTLIDKLKYTIQDFNNNTIEILSSTQNNQQRVKDKFNRKEINPLFKNHRKSSVDGIILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KN56</accession>
    <name>A0A8S1KN56_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0250010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8056698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KN56"/>
    <dbReference type="OMA" id="LVNQIMQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8056698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="118" mass="14044" checksum="288C4A75FD7F8D6D" modified="2022-10-12" version="1">MQQNSKIHKQKSNIKRVKWNQESSDLFNKLYHMFLGDLNMIHSYFQQHTDFKFTKKQIKKQFIQQVQNLTKSNSKSMDEEIYAKIFDSQQQSLSNSNYCEDPEQSALSDIKSYQSSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KRG2</accession>
    <name>A0A8S1KRG2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0270012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KRG2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8058039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="189" mass="22618" checksum="B350B380318E1E4C" modified="2022-10-12" version="1">MKSITQLNVYVELTLASQVYQIKSNSHQNKLNRQQKIFQNTKNNKQIQDQLIIYKFKNLQHKRIDFTKTKASKFRKTNSVAIHFRTDIAIPNNISLSFTDQRQLGQEIYLKLLKLSIHFSQILNYRKTLNIQQTFQSRAIPKLRHRRRIMKQQKEGTFVYKEQIQNFFSNKNKLIKDVEDLSFDRGKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KT19</accession>
    <name>A0A8S1KT19_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0230376</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KT19"/>
    <dbReference type="OMA" id="SENCWAM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="242"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="286"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="332"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8055826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="60418" checksum="B53B53ACC305719C" modified="2022-10-12" version="1">MIQNCELVCFQNHDKPVCSVIVEKWVKKDERLLCKECLKNFDQKINIKGLNKIKEMIEIKLSENNEKKQYFLSLKLQEIEQLTNDLNQLKQKLIITFDFILGDLKNCMDDLAKEQQINNTYRLIDELEKLLNQGSYENEFMQFIDQIIFQNNNGIKKVLSKISQLTDIYKEANNIIKNSFKKIINIPQTEIQLNLINKDIKQNKNCYAIAFNQNGQIMASGCGKDIKLWNFNNGKLKEIQTLSSHKSDVSCLIFSKLSDNFVSGSFDSTIKIWKQFKGQEWKSLQSKEDNNPIFCLILQKNEKVLISGSEQYSINVWDIDFELNRLNLSYSLIKHTQSVCSLSLNESETFLVSCGNDQQIIVWMREQNNQWGFKQIVTQSIKEIGTKICFIKENQFIWICGNQNGKDCICFFELQNEQFQEQFNQQINLISNNQESDQNLFPIIFNKKKKIIFLRHKFIIYILSQQFHNKYKIASKLKFDNYLIQGSMTNDAEFIVVWEKSNSSYLIYQINQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KTD0</accession>
    <name>A0A8S1KTD0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T0250292</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDM010000023">
        <property type="protein sequence ID" value="CAD8057155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KTD0"/>
    <dbReference type="OMA" id="HCNENIA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF306">
        <property type="entry name" value="PROTEIN S-ACYLTRANSFERASE 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="29"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="175"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="228"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8057155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="241" mass="28801" checksum="613A3824F7115FB5" modified="2022-10-12" version="1">MLLRLPTQIQLMIQQSHKEMDQIYQELYDYFCQFCDSYVSGTTKHCKVCERCVSDFDHHCKWLNNCIGKKNYQEFFKLLVFVSLFGITFIIFAIFSFFFESPQMMIWIWINVGLVSILFLLNFNLMIFHFWLKFKGITTYAWIMQNRQRNFQQQQVQIETPPRFCCGSKKLRKTQVNPRIDEEDDENKQQNIEVKQNDQQSQIKQQNVEIEGDSDSIPDKNSKRKCSSHTINPQINSQLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KW93</accession>
    <name>A0A8S1KW93_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0260126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000025">
        <property type="protein sequence ID" value="CAD8057602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KW93"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8057602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="72" mass="8774" checksum="D838B73420E13123" modified="2022-10-12" version="1">MIKQLETLNIDSQAINLPIQPRQNKQIQMKFRKSLKRQLTQIQNLMICNWTQSIKLFQHKYFPNKKIIMQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXC1</accession>
    <name>A0A8S1KXC1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0290217</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXC1"/>
    <dbReference type="OMA" id="TTLCLCG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="6">
        <location>
            <begin position="121"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="201"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8059888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="259" mass="29900" checksum="D5ED93371C9BC5D6" modified="2022-10-12" version="1">MPKAPKTSKAKKIQKKVADRKKNPLFVKEAKNFRIGNDVQPKRDLSRYVRWPRYILLHRQKKILLQRIKVPAAIHQFSKTLDKNQSSKVYALLKKYAPETKTEKKQRLVKAAESKAQNQKTDSKKVTVLKFGLNHVTTLVETKKAKLVLIAYDVDPIELVVWLPQLCRRQEVPFAFVKNKARLGALVHQKTATCVALTEVRKEDQAEFDNLARDLRQHYNENHELLRTIGGGQVGIKSRHQQEALKKALELEELKKTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KXD0</accession>
    <name>A0A8S1KXD0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0270280</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KXD0"/>
    <dbReference type="OMA" id="FCYIITI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="95"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8058555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="308" mass="36704" checksum="F69AB2F2A7803B7B" modified="2022-10-12" version="1">MLQEKINNGKQKSKKNYLGLGLVVLVQFLLIWGLYTTFIYQNQNSLSNCIRNLAIALWVISEICYIRTQFSDPGEILQKTVPLKLLHDQQLLQYYQNICNKCNSWKPPRAHHCRRCNKCIFKMDHHCEWTNNCIGALNQKYFVLFLLYVFCYIITIFSIHIIGIYSYIIRIDQQNLWNLITNNKFQIFEVFLFIILCTFFSFFVISMLWDQIQVIRDNQTVVESLQGKFGRQQSFFLNMKQFMGNQQWYYWILPTTPILKLNYAEIVYGEQLVNLGTSYLEDIIYDEKNPKSAYFAEFMVDNYKKKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KYB9</accession>
    <name>A0A8S1KYB9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="16">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPRIM_AZ9-3.1.T0280352</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21 16">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Na(+)(in) + ATP + H2O = Na(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:14634"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="16"/>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008554">
        <property type="term" value="F:P-type sodium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000317">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000190">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 15 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 16" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1323"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1356"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1421"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1451"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1482"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1520"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="17">
        <location>
            <begin position="41"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="18">
        <location>
            <begin position="1292"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="13">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="531"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="574"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="597"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="621"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="704"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="705"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="706"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1240"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1246"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="1266"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1269"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="1270"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8059341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1566" mass="181038" checksum="F79BAC715A93158B" modified="2022-10-12" version="1">MNIVEYRKHQNENQIRTCCCKRRAPWELQPDRKILIGTADKNIPNNRVETSKYNMLTFLPKNSLEQFRKASNLYFLLLGIFQILPPVTTTDGQPTVYLPLSFIIMVSMIKDFFEDFKRHRADDEENNRTVQKYSLRTGVFEYDKWQNVYVGDIIRIANKQRIPADIIILATSKGGECFVETKNLDGETNLKPKYAHPQLQTLYRQLNEKELPYNLFVTMDFERQNPLMYKFKGSFNIVNENKELPKEPLNYENFLERGCSLQNTDWILAVTVYTGHDTKIMMNSIIGKMKYSTVEKLMSKQILWVFLFLLFECIFASTYYNVWYQRNVNELQSYLNIDQNAPENNSFYNFILRFAMWFLLLGNSVPISLLVTLETVKFFQAQMIQWDKNYLTFDRPAAVHSSNLSEELGVIEYIFSDKTGTLTQNIMKFKSIIIDDIVYGDIEEQPQQEIIDEKPFKEKQRIVSQNVDFTDDTLYSELVSEFMNKKSAKNFSNNDHVFLTLLCLSLCHTIQTELVENIESEIQYNASSPDELALVSFAAEMGFKYIGKEDNVMKVYIKHSKETLKFQIQQIIEFNSTRKRMSIVLKDQNGRLTLFCKGADNKNILFEIKKQLQEYATIGLRTLVLGYKELEQKEYEKFLSEYNRAQAILDDDQLRESLMNQLEEMIENNLQLLCATAIEDKLQDKVGEVIADLKSAGINVWVLTGDKIETAINIGFSCKLLTDQVKRFIIDGDVEGQVERQLAKVTNYINDHPDEPLSLIVSGVALVIIIHNFKEVFFKVALKANAVMACRVSPKQKQEIVNLVRRLTGKITLAIGDGANDVAMITQAHVGIGIRGLEGLVIMLLENLNIQEGCCFIQDMNHIGKXIDDIVYGDIEEQPQQEIIDEKPFKEKQRIVSQNVDFTDDTLYSELVSEFMNKKSAKNFSNNDHVFLTLLCLSLCHTIQTELVENIESEIQYNASSPDELALVSFAAEMGFKYIGKEDNVMKVYIKHSKETLKFQIQQIIEFNSTRKRMSIVLKDQNGRLTLFCKGADNVILSMLQDFDENDSIQKNILFEIKKQLQEYATIGLRTLVLGYKELEQKEYEKFLSEYNRAQSILDDDQLRESLMNQLEEMIENNLQLLCATAIEDKLQDKVGEVIADLKSAGINVWVLTGDKIETAINIGFSCKLLTDQVKRFIIDGDVEGQVERQLAKVTNYINDHPDEPLSLIVSGVALVIIIHNFKEVFFKVALKANAVMACRVSPKQKQEIVNLVRRLTGKITLAIGDGANDVAMITQAHVGIGIRGLEGQQAAKASDYAIGEFKHLRRLLFYSGHESYRKNSNLILFNFYKNQLYIGAFFFFGFSNGFSGQNLYDQWLSQIFNVFFTSLPIILYALFDEKYPNSNYMQLVQDKANFLESRPDIYREYLTKPIFNLVSFWQQFLWGLLQAILLMVISFYAFEPISPHVHGQNSTYLEAGMTIMSAVVFIVNFKVLLLHNTNYPIIVYINIGSTTCFILFFLLFSQYPFFESFDLFQRLYECFNYYLAMILMLTVTNFFDLGRQRYNYFVEQYREQQQNNPPNQLEIEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0T0</accession>
    <name>A0A8S1L0T0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0310244</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L0T0"/>
    <dbReference type="OMA" id="RADNICA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665:SF9">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8061309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="31625" checksum="9D3014531D0DAC8F" modified="2022-10-12" version="1">MSEDRLLQEEVPYSQSIQYTQQKKNQGNFDEHQFEVQDNEKDELIRKIEALESKLKNTIDDKPYSELIRILIWEALGTAFFAYGIVCSRGNDVMLSVYLFGAIFLIGKITGGHVNPAVSMSFYSLNEISAFTMRIYWAAQVGGAIAGALAAFVIVGRVTSPYIETQPIQWMVADFCGEALGTFVFCLFIHIQVHPQTQLTENKLIGIGIIATALYFGRILTYHTGGCLNPAMGVGLGIFESLQDGNWDRLINIWIYIFGPFSGASLASEFYRSVYVGLLPKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2024-03-27" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L0V7</accession>
    <name>A0A8S1L0V7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0310254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000030">
        <property type="protein sequence ID" value="CAD8061329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="744"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="825"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1038"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1304"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1565"/>
            <end position="1599"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1631"/>
            <end position="1876"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8061329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1967" mass="236587" checksum="9CFD7AA9C3274A73" modified="2022-10-12" version="1">MFSQQTDQKEVITNFPENTDKILESQNEFFDNKTRIILMAIEIERLHKILNSKIDYYHQKMQDYIKRELFYKGQIVEYLQRNTELNQQILEKNALISQNIKENSQQEMKMLITQQCLQFEQELKSSQLKNLQLQKENQELRSQFEDAPQLRKKLYDLEQLYSQLLIEKNEEIKQLSQNNQEYTLKNSQLDTLNQLLQYKELELLEWQYSSQQTNLIHENQRLKKELKFLNDKLNLLLINNDHLKVKQDDIQNLNYCQTTISNKIFRQANDIRNQTPRRQQINPTQDQLFNRQNTPSNQIQPSQIPINLQSLSQPQRDNINNQNDNNNYQFKELIEQILFIQNQKTQSKQSIQNQISLLIKVLNLKQQEISQFLYQKNNYVINQKLSASPQFIANPQRKKFQKYYSLSPNNCYSQPYVILQPRSRSPINQYHKGIKTETTFHSYSNMLRQQITPIVIKPQNIVTYNYNKPQTQIHYHFSPIQKTVVKQNFQNPPYIVRNTQPNEAVQFYQPIKINVNQPNLRSFDKQEQNLNENMNQPEFNVYTTEFQVNSQENIKKGKSPQKTLINFPKQDVINDKSQQQQSHLDGSLPQNVFVSNGDQSLSQKSFEIYQEDQIFNKITLQDPVSIIKQKLNGQAQSQENMKDSFNIIYQQFQEFRSIFKQKLYNEEQEFQKTYSNSLEQLQKLENIILVLVNEKTQQISNSRDNKFNRISYEKIETDQQKEYQSYKIEQPNSDYFQIKKQTQINEANDFLQQITKQKKLLNEQKNHFKQLISLQDEIQNKYEPLVSLLFQKESELNKELIEIKQDKEMQKLNLTLRLQVQSEEITKFKQQNSYLQQKLNEYKNELKDIQLKLKFEHDQHQITKQIVQSLQQEYQDSTSEIQKRNSYLEQQIKQTEQQLFQAQTNSKKQKLSYAKETQTILIENQQIIQQQIQNDQIEIENLKQKLCFEQEFTNQLNDSIYALQQQIQTQEQLLQVQKLNNSNQFDEIQNLTKQKIEITQQLKMFQVKYLSLYEQLDQIQKQKDVDQNNEQIQNIDIIKNLNFQIEDMKQINQFLSNQISQIQSNNEIQISAQNTVLSSKIALLEKQLAAEKHLKEQANIRIMQLLKQYQDLQNHIQQDEISDFKEQIFKLSNQQRLQQQQKENLIESFEQNLNQLKQQINELTHQNSNFQEQIKDFETKQLLHLQLQQQYANQTQLINQQQQQIVILQQQNNQNQNDLQKLRQNLKLDIQDFEDHQNDQIIYLKQQIDELNLYIQNQQRDINYLESQIQFEQQEKNIITQNKTNQDRLINQMQEENCQLQNQIQIQQNNIEQLSKNIRQSNTIHNQLSQTEEYYQNQNEIPKLENQQLKQDYQILIQEYQKLKDALYQITEEKNKLEMILNYQNQVQESKFQEINQLQSIIKIDKNEEKDQLNQNEFNNKQYQIKDLDIQISAALVKLDEFDDLNIRSEQIKQDLQIQIQRLKQQTLYIQQLRTEQDKNKDNIQQKYPSINFNQKIDELIQIIQVLIISIGDHIQLNSKIVMNTQKLYQQLIEINKIIESFQFQNQTLKREGNFTEQPNYSTAQKDYQKQCTELIIENDQLKLKLNQYQRLLIQLQDEYYKQGSALDNITKEYQQIINKNEYKVIKLIDFEQLNNTINKQEKQIEEYLDQIQYLQNHFDQEQRKQLEGNARFNNKIQENQKQMDNQIQIIQENIKLKQEISNLQSELQKQNYQEDLREEQKFIQKQNQEEYIQLKQEAQRLYQRLLQMSNSNNILKEEKELEQQKYQRECKQIQENLEKHKNENIQYKLQIDRLSKQLQEQKSKSQSNLQQEFNKLQYTLDASLKQQKQINDEKRVIQAQVQQQQEQLANQLNIINQLKKEISKQEQIISQFSQQNNIQSSFVQEVTFFDDSKNSSPFEFKIKQLQNENSHLHQLLIDTENDYSHKIKLQQLQIQGLNDKLREMVYELNNKNPKVDEFVQINNQNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L4F2</accession>
    <name>A0A8S1L4F2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0300137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L4F2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="67" mass="7978" checksum="4C52004443B68B1C" modified="2022-10-12" version="1">MINLNMDYVKKIQLDCQQNLVILDWIIQNQSQIFGINYIRIFLILVVSISFSKLKKYPQLVHLLKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L783</accession>
    <name>A0A8S1L783_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0340239</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000033">
        <property type="protein sequence ID" value="CAD8063277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L783"/>
    <dbReference type="OMA" id="RDCKWTI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8063277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="31183" checksum="F1A3F69B7CC155B7" modified="2022-10-12" version="1">MLKKEPNRQIDLTLVLNATKQKIKISVGIHTTLKEIANLLAEELTLDVFRTRISFQETYKTTKLDESKTLKQLGISNDNQQLFANVETIQTKTIGRVTMAKTFQKPIERFRVTDLDLVPFHPGQIQPFEVEGLTLEAVCINAKCINFQREIVFPIGFGTFSFQRIMRDVKCDMCPYRALGINPSVLVRELLFRDCKWTISGLKKGGNGVFDQCLNRWVKVKGRDSVAFPQIVGQDKWKEVWIEIKPIGVGTQKHPFLIQPDCLDDFYYSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L9N6</accession>
    <name>A0A8S1L9N6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0360327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L9N6"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="25982" checksum="93290FF146B29020" modified="2022-10-12" version="1">MLYLNEKQTTLTHSTLNISLFYQSYKVGIFQETYWENKRRSVLNRNQKYFRQHLNTLESQQKDFYVKRISPNHFTLTILRFLILLKIFEKSNSQAIIVQYLIIASSIIKVYSFISKNGQYETFQLQNYFWMNYLVINYYLRYHYLIIIRRKMSKAPQKNLQLFNLVDWLKVENMELHVLRGSKESYDEYTEDKYSTQIIKSQQIQIEVFLNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LAD7</accession>
    <name>A0A8S1LAD7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0290292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8059974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="282" mass="33681" checksum="D10479CC048C04FB" modified="2022-10-12" version="1">MLYSRPKYLPKCNNHPCETIESFENAYKNNLIQWAKKQNVNEIAVVSKKITTFIIRMYIYDLQVERCNYALDEQNPRFPSITEDIKKVCLLRKRRSHEEEKLHLEQKLLSKIYIVDQDEIFEKFCLQGSLEDNINLLIECSNGLAFSSQPKVSYNCITKQYIVNDAIWFNPKTYQSCTGWITNEFEKAIALLYYQHQQDCIEENNLNKDDDLKRFWIQDFDLRTKILNQIPQICKEFKNAPKKKNTKQEDILWTQLIXNIYISIHFVILSSIYSIHYYTNQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LAD8</accession>
    <name>A0A8S1LAD8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0350281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8064069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LAD8"/>
    <dbReference type="OMA" id="IQWFEGF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001034">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="79"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="452" mass="52731" checksum="629B0C98DD043092" modified="2022-10-12" version="1">MIVENVKKSKFSFSRKSESDEQCKLDQFKNKLQGDVLQFLKNTKQDDQSLQSFLQRIGIILEGDEENYILDIKEYHEKYIEGDILGEGCIGLVKSVKRKSDGFEFACKTVKTDSEEIVKKMILEFKNLKKLNHPHIVSMKEIYIQWFEGFQSTGMVYVIMEKIDGREMFEVIQQQKQYSETIARVLFIQILEAIKYMHENYCCHRDLKPNNILCAHDGKSIKITDFNVSKFNDSYKEFGDLNQHGKIEMWTYTGTVAFSAPEIFSGNLYNEQVDLWSAGVILYVMLSGELPFNSEYLNDLIEQIRQCKYDFAGIIWAQISESAKDLITNLLQLDPDKRFTPEQALNHPWITHEQSNSDIPRYQLQINMARILRVKNINQESKIKQICYLFGAGDIWKRHSLGQDTNLDLYDNLKKYRSIDISDSWKNAEIKVIKDTNKKGVYTIDYPFSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LBI3</accession>
    <name>A0A8S1LBI3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0350152</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000034">
        <property type="protein sequence ID" value="CAD8063811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LBI3"/>
    <dbReference type="OMA" id="ETMRNTT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000089">
        <property type="entry name" value="Cyclin, N-terminal domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="100"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8063811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="285" mass="33450" checksum="91CF2FDA6A0B2C1D" modified="2022-10-12" version="1">MHQNQGGRVFGRHLDNFAEKAFKDAKDRRNGLIDEIGGKENVINDAVLTKDGWFKIDCPQKVLQYQQEIYEFLHQREQESQGFQNNQRKINETMRNTTIRFVIKQAKLYNLKSETLFQTVDLIDQAIQYINPEVEKLELIAITCLFIASKYEEIYPPPLGVLIRGTELRVREVIEMEKEILYKLNFNVISDNTLIWLQLIGELLGCNKRYLELIKQRSMYLAELSLSNDRFLSIKKSTIALTIFLAVEIQFGYQKTQFQWDRLSQHSKPSKDSKPLKLFAYCLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LMF8</accession>
    <name>A0A8S1LMF8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0400237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8067351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LMF8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8067351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14716" checksum="3F780266B1185E0C" modified="2022-10-12" version="1">MISSINFFTIQYTIINLCIICCLIIIICLYTDTFNNFNCFNNTLVSIIQRYQIQVPIDPNTITILNMSKQLIQVFDTDSFICKISPILQLFRISLSLFMHQDQKEREIIYGKYSIVWLDAETNPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LN51</accession>
    <name>A0A8S1LN51_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0440007</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LN51"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="496"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="279"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="324"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="370"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="178"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8069400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="521" mass="61477" checksum="FA7CD1FE79D64F35" modified="2022-10-12" version="1">MFKSSMIEKEKDLKCANKHKLPILMVVLDPKLELNQRLLCSDCIKHFTTQYSTIGYELVTKQIKKNQKQKVQTIEKTIQLNIKQVEELLGNINKLKSTIEFQLDEIIGYSGGWIKNLLSVGHEFSQYSFHKELENLISKQLENSIDKSLLINEINKVNSFWNVKINEKLEQFSSFKVYQKCQEILQNLNQTIQIKQEENQQQKVSQQNNLQSQLEQLNTNQLVSESQINLQLIDDNIKQKSKCYAIAFNKDGTQMVSMSHSDIKVWNMIDGKLNYQLNLQGHTNYIYCLLYSKKQDSFISSSNDKTIICWKQIKQNEWKASQHYKEHKGWIGTMIINNQENQLYSAGEDQKIIVWKVDFNQNELTYMYQLDKHTKCIRAMSLNEYENILVSSGDDQQIIIWEYGQGDKFYFKQVFNQILNCSGCHIKFIKNNKFIWIPYSKELNCICIFDFKDGIFNEDIDNRIQLLNSNKNLGCALFPIVYLKDKNILFIRSKNIILTSIGIYFFLQYTYFFKKMIMKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LNG6</accession>
    <name>A0A8S1LNG6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">PPRIM_AZ9-3.1.T0410218</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8067892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LNG6"/>
    <dbReference type="OMA" id="YTEFIIV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000632">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000522">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="62"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="360"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="402"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="438"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="475"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="CAD8067892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="503" mass="58397" checksum="9808D2850E335840" modified="2022-10-12" version="1">MGSYCCAQEVANSNETQIETRPIDFNSTDKKFSCHATKKITLLKVSPDLFIPMKRQDIYSVYSLGKALGEGAYGQVSTVTNKRTGMIRAMKAIRKDSLFEEEEQKLFQEMNILKDLDHPNIVKLCELFQDEKYYYLVTEYLQGGELFDRIQKAKTFSEKDAAHIMRQILSGVAYCHTKQIVHRDLKPENIVFTSKDEDAQLKIIDFGTSRRFESNKKMTKRLGTPYYIAPEVLLKQYNEKCDVWSCGVILFILLAGYPPFYGKKEVDIYQKIVKAHVSFHTAEWSRVSEPAKQLILKMLCKNVDQRISAQDALNDAWMIQHNQTNLVDQQFLKNISQFSAKSKLKQSLLTFMACQMIQQNEVEDIQKKFKELDQNGDGTVSKEELIKAFQEKLLSKDYFVESLDEQMDKVIKQIDTNLSGKIDYTEFIIVCLQQQRMLTEEKIKQAFKILDMDGNNFISKDEFQQVMEGVDDILWNEFLEECDEDKDGKISEEEFIQIIMNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LQ89</accession>
    <name>A0A8S1LQ89_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cathepsin L</fullName>
            <ecNumber evidence="10">3.4.22.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPRIM_AZ9-3.1.T0360350</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in extracellular digestion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.</text>
            <dbReference type="EC" id="3.4.22.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.15" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LQ89"/>
    <dbReference type="OMA" id="HEFNEMR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000104">
        <property type="entry name" value="Cathepsin L 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035865231" description="cathepsin L" evidence="12">
        <location>
            <begin position="23"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="33"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="116"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8064884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="309" mass="33831" checksum="011F9AC782E31CAC" modified="2022-10-12" version="1">MQKTLLVAGLTLLLSTIGYIQNQQPVDEVSMVFEQFKIQYTKSYAYVEEAYRRAIFEQNYAKILAHNADPTQTYTVGVNQFTDLTQDEFASIYLTYTPPEGWQPSYEEVVQEGVEPNDSVDWRSQVRVKDQGNCGSCWAFSAVGAVEAFLKVTKGENHNLSEQQLVDCDKASSGCNGGYPDKALLYIKANGSTTESSYPYAAKNQACKTATGSTKISGVQNIAKTGLQAAIKQYPISVCVDASNWSGYKSGVFSNCNKNINHAVMAVGYDASGNWIIKNSWATSWGQSGFMYLQAGDTCGVTQLAVRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LSA5</accession>
    <name>A0A8S1LSA5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0440289</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LSA5"/>
    <dbReference type="OMA" id="VIYSECE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035803618" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8069889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="546" mass="62652" checksum="50558C233DD89684" modified="2022-10-12" version="1">MKKQIVLLTLFIVLTYSQRGTLVTHYDLDGNLLSEEFMAPIEDEITYYDSNESAIDSSMDQQTQNQPNPTSVQESQDSMSIDVPIQISNTLDPVQTDQQQTNLIGSSFEIQPVVNQQPKIETSIEQQHNLEQQVEGELHSQEVHVNQNLDLTTQQQQIPQSKFSQEQDEQQIQKVDMQLQPVVVQSDQMVDLQQDFDQQTNSSDINNQILNTQSTPDKQNEKDKELQQNKVDPLNLSKPDDEVNKQEQGTQNQIIQDMPIQEQVIVQSGNEIHIDSNTSEVKFDSLGSVQEQQDKQSEQVHIQTTNANDQFEVTQNFGRIIQDMIPESDDDTLIQEQTLEDQQSQTKANFQPSQIEEDINFNNENEQSNQLQETQVKPDNQLPLVVGNEQQEQQIETDVQEITQESFQAKKDIPEELKQINVNPSDNNMKKQGVNKINNPFEDDGLKLSSEIIQNQCIVIYSECEYQGLALEVCDSLKDIEKFKHKIKSIYIPKGYGLTIFENQNFNGQSHRYTSSQACLQREVSFIQLQMDQSILIKHQNLRGQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LY07</accession>
    <name>A0A8S1LY07_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0440156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000044">
        <property type="protein sequence ID" value="CAD8069686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LY07"/>
    <dbReference type="OMA" id="LQYWIYL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8069686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="255" mass="30951" checksum="D778815193ADE323" modified="2022-10-12" version="1">MVLRRFTQQYKSIYAFCKVESYTIKPQNTVIDYKTLTYNPQQKIKFDSSNKSLLYYYKPLLWTYQLQYWMYLFPMVLIGNVIKHNPFYSTFPAALPMMFTVEFWLFLRWFKKFKYLRYVVSEVYLHKDGQTVEVVYEKKFWRKVKEQNVTQIFYVTNLKTLIGDDKRPLKGNLFPEQWPMKKEMSKKWRWSFVKYYLNQNNFLVFPTNPNYINGEILIAVMSGKIINTSKQYVEDIEGDMLEMVEEGKIKSDIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZN5</accession>
    <name>A0A8S1LZN5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0510106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZN5"/>
    <dbReference type="OMA" id="NIQRNQM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8073520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="233" mass="27441" checksum="A4AA7A68049559EC" modified="2022-10-12" version="1">MSLNTSMPYYRNSSREVKTIKEIVQAEVIKSARLLSRDKVKVKQNTSIGDLNRSKRKINNSFYDNRNNSQIENSNILSGYLANAFHERKENIQKIQMQENTNKNKFDENKQREIKYLMRIAALEKESSKLTELATKLKKENQLLKQQNNQDLIIYQLQEALNINKNENQNLIKQITQLQNSNTNYFNNSVYRIQKHQNQNTNQTVYNINNITQDNLKFKEYIPSYLVALSLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M4U1</accession>
    <name>A0A8S1M4U1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0500106</name>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0500107</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000050">
        <property type="protein sequence ID" value="CAD8072905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M4U1"/>
    <dbReference type="OMA" id="NTEYQEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8072903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8072905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="19303" checksum="047A1525D0B368F0" modified="2022-10-12" version="1">MNREYQEQEIDLERKSQQNSEDTNNQLFSIGFAIIYNCIWGILFCFFRHRYYEEECKVINFWSLVAEIYLFTVAIYKLAIELPVYYKALGRWKHKLFEIAEKVEFVLSIIVLIGLSYAYFQFEDCYGLRNFVLFYLIVTYLVLGIYLVSLLLLITNKSNNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M6H1</accession>
    <name>A0A8S1M6H1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0540160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M6H1"/>
    <dbReference type="OMA" id="EQIFCLI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8075349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="399" mass="48175" checksum="457B3340AF40A799" modified="2022-10-12" version="1">MGIIQPKLNNFFNLQIKYHLQNFESYTIKLCEIFEGQDLNPQHKEDLTKLFKLLQIQYNEELEIDRYEFSIIIILFTKITLEHKLEQIFCLITQTGILTFQEFYWLTQSFIRATCKVNLLSIPIFDQILKLVQKMYQQMRKDIDENLMLIEVIDYIKSNSEISQFLLQFYKIQTYENAIRQQELQLQSYIKLFNSSIGLTRKYVNIDQLMKNWNAWNTDKQHQQYLLSLMLKQKQNKQKQTNFIKLSQFLLVISNYLSYIVNDIDNTNNLSIDFITYLVYAQEEIIITADRFEKIKYALCKDRQFITLHMWMDYQCIQECNKFESQLRSLVRFYEQIYSNKDFNQLIQKIQKLVKKNEKSNQTQSQYINKFLQNWLQNPGLYNIGSIELKLKIEQLLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MA02</accession>
    <name>A0A8S1MA02_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0480226</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000048">
        <property type="protein sequence ID" value="CAD8072114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MA02"/>
    <dbReference type="OMA" id="CNENTDA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00124">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF706">
        <property type="entry name" value="DILUTE CLASS UNCONVENTIONAL MYOSIN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0518">Myosin</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="4">
        <location>
            <begin position="38"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="149"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8072114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="373" mass="43292" checksum="114F3D040A3C9564" modified="2022-10-12" version="1">MSYYDEIWIEDVKEVCKIERLKEDNFASKLRVDPLAKEGIQDLLLLSDLNEFNCLHNILIRYLRKQIYTQIGSPILISINPYVFLPNIYNQETLANYRVKLGQYQKTLKRSIRENEFEPHLFKIAQLSLDQLFETPTKELRICSMMISGESGSGKTESTKILLKYLAAESKSGLFNTIQHGQTIQQQIFASNPVLEAFGNAKTARNDNSSRFGKFMHLYFNPDIKGVSAAKIDNYLLEKSRVVKINKQERNYHIFYQIIAFQIPELKGLKSPYQYEYLNGGYLSSLRTEKSEFVETDKCLDNLNFSPNQKRQIYQTIAGLQHLGNVKVEYDSKKSTLRLDDSLRFASYLRRIQIQKLEDLICKVITSDGKERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MBV9</accession>
    <name>A0A8S1MBV9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0540075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000054">
        <property type="protein sequence ID" value="CAD8075183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MBV9"/>
    <dbReference type="OMA" id="LICLGQC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035900193" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8075183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="13082" checksum="83335E6E616E1AC4" modified="2022-10-12" version="1">MIKIGIFLVLICLGQCQDLLTSIKNYADGIAQSNITQKILIDFAPLLLPLTILSIILIQLMKICFKRQKFYDKLPQTEQQANKKTIKLPLKHKMQLYFMRYYCQFLRLFTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MEG0</accession>
    <name>A0A8S1MEG0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL6 alpha-beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T0550124</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MEG0"/>
    <dbReference type="OMA" id="CASHITN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000008">
        <property type="entry name" value="50S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000004">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_uL6_CS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="5">
        <location>
            <begin position="12"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="5">
        <location>
            <begin position="118"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8075885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="196" mass="22524" checksum="AC28143EE508F612" modified="2022-10-12" version="1">MKYILTEEHVQIPEKIEITQKSKIVKVKGPRGELSKNFRHSALDIQISKKVNKKTNVAKSRVSVRMWQSYRKQRCQVNSVASQIRNMIRGVTTGYKFKMVLAYAHFPIIINLLDDGKTIEIKNFLGEKIIRTIRCLPGVKITRNESEEKNVLTLQGNDLNNVSLTCALIHQACAVKNKDIRQFLDGIYVSEKRLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MN70</accession>
    <name>A0A8S1MN70_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPRIM_AZ9-3.1.T0640162</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000066">
        <property type="protein sequence ID" value="CAD8080782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MN70"/>
    <dbReference type="OMA" id="KLECRAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12236">
        <property type="entry name" value="RRM_snRNP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001833">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034143">
        <property type="entry name" value="snRNP70_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051183">
        <property type="entry name" value="U1_U11-U12_snRNP_70-35kDa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952:SF5">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="138"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="235"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="235"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="294"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8080782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="307" mass="36845" checksum="B926B93415230F7D" modified="2022-10-12" version="1">MYYQAQPPMMPLAHMMNNPVQMQQATNIRKPDFPPDMQPIFVIPQIAHIKSQPKPKCRPYDPILGLGRKAEWMIDKFEDEYEQMQREEKEKDIVKPSRLPPKILKRLEKKHLHNEELKKLLQLWNPFEDPNITSEPYKTLFVSRLNYATTDKKLRKEFEEYGPIKSIRIIRDSTQDKPRGYAFIEYESKNSVKQAYKYAVDKRIDGRKLVVDIERGRTILKWRPRRLGGGLGELRRSRSEEISKKPKEEHVEKDEEDKRRKSKKEEVKPSKKPRERSRSKSVKKHKKREESPIKKQKRREKSKKRYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MPG2</accession>
    <name>A0A8S1MPG2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cathepsin propeptide inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0630198</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8080311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MPG2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035795554" description="Cathepsin propeptide inhibitor domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="2">
        <location>
            <begin position="36"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8080311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="105" mass="12625" checksum="3448D3C67CD401D3" modified="2022-10-12" version="1">MEKSLISVGLLLLIGAGLYLKQNGEDLYELQKVKQFNDWKIQYNKKFSSEQEEMYRYLVFQQNAQLIEAHNNDKSDQLICRLDRIRICIKIFEFETQEQYQIKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MQK8</accession>
    <name>A0A8S1MQK8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0660097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000068">
        <property type="protein sequence ID" value="CAD8081642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MQK8"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8081642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="43" mass="4659" checksum="68E87BDD48CED5BA" modified="2022-10-12" version="1">MEITVTKNDGTSLVVHSKLNGDGPFNNQSAQKALEKLVAFVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MSA5</accession>
    <name>A0A8S1MSA5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0630010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000064">
        <property type="protein sequence ID" value="CAD8079953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MSA5"/>
    <dbReference type="OMA" id="ATLFHII"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8079953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="156" mass="18386" checksum="40F2E4389CB6A336" modified="2022-10-12" version="1">MIEFLRKKGKSENKYDFESRIFLSLDLCRKFIYHFVLIFFQECPTLQFLCLSIISLSQCLIISKYEESSILDKIISILNEGTIAMFSLTCFPYNDINRIYFSNETIKSTGFIHMGLLLLNLMNVLFKQLLIKLQFILKGLYARKQPEQIKNIILNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MUF9</accession>
    <name>A0A8S1MUF9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0710007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8083810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MUF9"/>
    <dbReference type="OMA" id="RIENTYM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="178"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="345"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="600"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8083810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="798" mass="94905" checksum="4B068CDB2C496752" modified="2022-10-12" version="1">MKNNIPQSPSPYKLKDSNLNYDTQQYQELMKNFENLIKVTQDKLYSNQKKIEETCKCSIEDQYQALLMENENLKQLLSELQMKLEHDNSNVKPIEQQQQFESFMKEAVLKSEQQNELIERLQQQTFEFQQQLLIEKGQHCQTQKQLSDIQEEFHNYKKERTLQQCYDFEVTEKITQGYSELVKENQKQKQELKELQLDIDKLRIALNQAYISLSKQQQQHKISQRIINQEEIQTTGTQTEAIIYQQHEILKERINNQMYNLEKFISQYLDKKKMRNKSANENKFYTDLSCWVEQAKYELISLKQQITALVTEKEHQKEQLIAVKQDFEQVQNSLIQETQVLKSEIKSQNDKYQILLEDFNRAQNKYIEIAEEKKLTHNIRNQLERELNELKLKHEQLILESNSYDTQFKIITQSYQSDKKLYEDQLDKLQQKNLSLESELNQSLLQHEQIIKQLQVANINIQQLQQQLQESQEIANHQSEQFQSTQYKITQIIKEKEQLLITNMELEQNQQLLKENINKLKEFQSKSDKEKQSNQNLIYEMEKEKEDLKIYCQKLQQDLTDRNVQLTSLKEQVSNMEYQLYLTPSDDKSRIENTYMSSKIEDLESILKEKDQKIEDLERLYHLTNYELRILQDYQSTFEKDLDFYKTKVSKLEQELIEQHNVLSISKTSVNSNIPINKFDEQIKIMSSPISSLKIDSQSNNGSPQIQVKILSGEIQRLSQIVKIQQKKLLDLQYFIEKMITSNEQNRLLAKQILLQASQCGEINGYNLFEKVNLNTQISVNSCETFGINVQSIEKHEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MWY9</accession>
    <name>A0A8S1MWY9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0680007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000071">
        <property type="protein sequence ID" value="CAD8082541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MWY9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8082541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="254" mass="31051" checksum="5A991C4E71C84E4B" modified="2022-10-12" version="1">MSIYKHYHQRRGMRLWPKKNGSEIWQECRTENQRLKELLEHSNYIDHTTRDLKNILETQLETNIKKQLVNETGSKKKHYGKQCRYIILMIIIYMELENSLIERDQKDQKRCKTLLFNLVIKKYSYTQYSTSTKRFKQDQVFGIKNQTVFFKNSQLNNDKNALISKKQISQFGQTILEIKWLIRTRIIRDKIQNFQKDINKFSRHKINNYLLLENIHNMMIIIKQFWENEYKYEESILALRVYDKDLKKQIIKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MXT6</accession>
    <name>A0A8S1MXT6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0740019</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MXT6"/>
    <dbReference type="OMA" id="FAMVGKY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000001">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050145">
        <property type="entry name" value="Centrin_CML-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23050">
        <property type="entry name" value="CALCIUM BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="59"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="133"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="169"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8085208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="205" mass="23874" checksum="EE23F7BCE7DACE41" modified="2022-10-12" version="1">MNKKKNTISAPSLPQNKINTQQLVSPPQQLSPTNSHQSFIPPEPKFDPNDYITATTTKRDIILYKEIFDFLDSNNNGVIQPMDLRKAFLIVGKYQPKKQILYQMIADFDSDQSGIIEFREFVRMMSTHPGEKDTDEDFINVFYQIDQDYKGYITIDDLRELSSECNENLKDEQLLSIIQSCDSEGNGTIREKQFVRYMKSLQKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N136</accession>
    <name>A0A8S1N136_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0740073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000077">
        <property type="protein sequence ID" value="CAD8085312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N136"/>
    <dbReference type="OMA" id="YEQEMNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="236"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8085312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="500" mass="59754" checksum="994ED7B1BF0795B8" modified="2022-10-12" version="1">MEEMKQNYLDRLNNLHQFLYCELPLKLMNHNEDETCIDLQNRLYTLIETGYIKETEQQLQQLIEENYKLHNKENDKQLINGLSTSEARYVKQIAELQHQRDLLQDELDEKNFILNQIQSKMEKFELLSDNLIKAEQAIKELSDENIEISQQLQYEQEMNQQYKNQIEELQQVVMNKETPKHRNISPSQLYQLHEQIGSLRIELSGLQAEVKIRVQILEQFRTQLMSDLVKGFHTMLSKTKQELQAETIEVKKQHQQLNEVNTQQINKLRKDVQFYKVLEEEYQNKIKEVLNQKSELESSIKLKDEQLQDIASKLDLIQQNIEQTEQNFQNQLIQAINSKEKYKIQLKNAKQGLQTLTDKVQSLLQFNQKQMKFIEDNISLLEQRIREENQELIDGLSQELNLSIETIKKQHTSIRQLELQCLKSNFQKVDPINKVTEQYRQSENINKHLDIENLKNDSSFIGERYMYQRTGRLDEQRKYLDDLKQLHRTTKSQLKLNRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1N4T6</accession>
    <name>A0A8S1N4T6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0710154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000074">
        <property type="protein sequence ID" value="CAD8084055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1N4T6"/>
    <dbReference type="OMA" id="NCCECCI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8084055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="224" mass="26047" checksum="5DFEA8C7495574CF" modified="2022-10-12" version="1">MGNNCCECCIKKNQKDNNYDANEKEIEQTVDYKIQKGPTHINVLYKNLQTSNCIVYVSCCDERGRNSQNSLLQKNEQVAEQLLKRNNQIGQLSEITIGKVFFFFFVLPLYLGEEQQILLLKQQIKQFAQIIIDRNLTEIAIEDIGVQRFGYPRQLVAKLIIETFANQLHKMVSLNRIDFMINDLKSKVLFEEEIQARREILIKGDSLLKIDQTDEKSLKQPLMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NA44</accession>
    <name>A0A8S1NA44_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0760195</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000079">
        <property type="protein sequence ID" value="CAD8086433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NA44"/>
    <dbReference type="OMA" id="YHESRID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002303">
        <property type="entry name" value="Rab_A02 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="174"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="177"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8086433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="225" mass="25373" checksum="7EDF519940D2E41F" modified="2022-10-12" version="1">MIKEYDYLFKLVIIGNSGVGKSSLLLRFADDQFSESYLTTIGVDFRFRTLPIDGKNVKLQIWDTAGQERFRTITSAYYKGADGIVMVYDVTQGQSFDDIDKFWLHEVESYGEKNVQLLVIGNKNDLDEQKQVETSKAEEYCKSHNMLFMECSAKTADHVNNAFLELSRKLMAKKDASQPQKTTNLTPNASQQSQSRGQTNTNTSQSNKLSANTSNQKKQKDGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NE33</accession>
    <name>A0A8S1NE33_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0730087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000076">
        <property type="protein sequence ID" value="CAD8084984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NE33"/>
    <dbReference type="OMA" id="RRYEGFF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8084984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="32947" checksum="14C390A428FED5EA" modified="2022-10-12" version="1">MGCCTSQSVSQRELVLHETYLNLHHLYRTEEDDEQTQRDWIIVEGGRYHGQIEDNKMHGWGILVMDDGRRYEGFFENNLFHGQGKLSAENYLYIGSFQQGECHGQGQLKTNTSIYEGKWEHNLQNGCGSERFADEAVYKGNYKDGKRHGKGYIEFVDSSYYRGDFFEGDIQGQGIFKWADGSEFRGQWVKNQFINGIMKWPDGRIYEGSYEDELKEGLGRFVWPDGRVYNGYWHQGKQHGYGLFTKDGITKRGEWNMGKRVRWIGEDENIEEFQQLYIQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NED9</accession>
    <name>A0A8S1NED9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPRIM_AZ9-3.1.T0870065</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8090970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NED9"/>
    <dbReference type="OMA" id="GPGIRNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000064">
        <property type="entry name" value="Protein phosphatase 2C 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="15">
        <location>
            <begin position="23"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8090970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="300" mass="33610" checksum="A4233C3B8A73F875" modified="2022-10-12" version="1">MGPYLSQPKRDKTTTTGQGKSVIFAASEMQGWRNTMEDAHIHKPDIVQDVSVFGVFDGHGGREVAYFVEKHFVEELTKNKNFKDQKFEEALKETFLRMDELLMTPEGQKELNQYKATDTDESYAGCTANVALIHKNTLYVANAGDSRSVLCRNNSNYDMSVDHKPDNPEEKTRIERAGGFVSDGRVNGNLNLSRALGDLEYKRDSKLRANEQLIIALPDVKKVELGPQDKFILMGCDGVFETLNHQELLKQVNSTLGQSQVTEELLKKAAEDLLDQLLAPDTSQGTGCDNMTNILVYLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NES2</accession>
    <name>A0A8S1NES2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0870130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000090">
        <property type="protein sequence ID" value="CAD8091100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NES2"/>
    <dbReference type="OMA" id="HCIRVVL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00299">
        <property type="entry name" value="GST_C_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050983">
        <property type="entry name" value="GST_Omega/HSP26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="83"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8091100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="25369" checksum="FC3B1A8972F34A9E" modified="2022-10-12" version="1">MSLVLVVSKTCPHCIRVVLTLNHLKIPYELKYIDMENTPDWFIKASPLEKVPILFVGDTILFESLVIIDYINTLVPQSLLPNDNLQRALNRARAEFSQEIIEILWSLFSAKTLETFNLHMKEMRQLFSQLEMWLQQTKFISDNILTLADFAYAPIFTILPALKQFIQFDIFDGFPRLREYVDNLHALPQLQMSLVDNYDLLIQKKIQSREPYFLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NJ22</accession>
    <name>A0A8S1NJ22_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPRIM_AZ9-3.1.T0820183</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000085">
        <property type="protein sequence ID" value="CAD8089005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NJ22"/>
    <dbReference type="OMA" id="ACEPIKR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06623">
        <property type="entry name" value="PKc_MAPKK_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000040">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF9">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="72"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8089005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="344" mass="38985" checksum="DC76EA75F143C654" modified="2022-10-12" version="1">MQTPDLKKKKPQVAKISLQSEQAQPVLNLSENGSLILKEFRLNGQGLKVTHTHDQWDFSLSESTHNLSLENLVTVGQLGQGASGQVEKVQDQVTGQYFAMKKIPVASDPQYLKQLSDELKLALECSSPYVVKCYGAFYKSGTLHIILEYMDVGSIDTLIKKVKNLNEPVMALLLYQILLGIDYLHNKKKIIHRDIKPQNILVNKKGEIKITDFGISGTIETMQQRKTYVGTAVYMSPERLNGEMYGKDSDIWSIGILTTESLMGKHPIQKTQFIDMVNEISTFNIENVQAKISAEMKNFISMCVKLKPEERATVDQLLNHKIILRTKKIDKMVFLQWLNQVTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NLY9</accession>
    <name>A0A8S1NLY9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0920142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NLY9"/>
    <dbReference type="OMA" id="RPNKEYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8093140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="107" mass="12766" checksum="671142303EDF5970" modified="2022-10-12" version="1">MNFIQRAAYRYGQNNISKLEQIGLSHTKSVSLSRPSRNFHPGQQAPNAYSEQFTYPKDYRPWTVNYKKDGAFWLVMGISWFAYFSYELAYLRRTEQEERPNKDYYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NX38</accession>
    <name>A0A8S1NX38_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0970079</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000100">
        <property type="protein sequence ID" value="CAD8094881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NX38"/>
    <dbReference type="OMA" id="WNQREQE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002303">
        <property type="entry name" value="Rab_A02 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="174"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="183"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8094881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="209" mass="23437" checksum="5B3BB5F830D9A403" modified="2022-10-12" version="1">MIKEYDYLFKLVIVGNSGVGKSSLLLRFSDDTFSDSYLTTIGVDFRFKTLEIDGKKVKLQIWDTAGQERFRTITSAYYKGADGIVMVYDVSSIGSFEDIDRFWINEVDSYAEKNVELLLLGNKSDIEDKAVTTQKALDYAAIRRMAHMEVSAKTADQVSKAFISLAKKLIAKKDSQGLKGQGPQRSQQTPGQKIGQQTDDNKKDKDKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1NZS5</accession>
    <name>A0A8S1NZS5_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0920139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000095">
        <property type="protein sequence ID" value="CAD8093134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1NZS5"/>
    <dbReference type="OMA" id="WRNSMIS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8093134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="73" mass="8660" checksum="5EDF839FA6CB0C33" modified="2022-10-12" version="1">MGGFQKYPIPRYVLSDAWLYNRQWFRHSVHVAIPTLILSFLIYRGGVTSQTQKMGEDYYVDPQDYQSPKKLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P135</accession>
    <name>A0A8S1P135_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1010024</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000104">
        <property type="protein sequence ID" value="CAD8096303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P135"/>
    <dbReference type="OMA" id="YHSASIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="265"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="409"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8096303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="576" mass="68991" checksum="FE09041AB8B813AB" modified="2022-10-12" version="1">MQAGKIYPIPNNLKNQNKELEKYDYMQCVGSNFEFTLLVSMLDCFLANENINKIQMLVTPQRPYSYSSQQIAQKIQNFIIKERNLLIFYQSVQNSDPLFKKTNQFLYEFCNNYEQPQSQQLKQKIIILTKIFQVSFFIFGELIRKVENSNTWIFIYQHQKQLYYYVKQFYEDFKPKIRSINFQKLKQSDVLYEKNKISENKNIDYKNYTDQIVKKRVIEESKDDERQITESQQYLSKRTFQNQVFGDQIEHQQNKDDPKNSIQFCLNCYDRSQTPLFINKNCGHSFCAKCIENITNLEKCYSQCQKQNCTKLIDSISAFYYCQNISTFRKEKILQLEFKQCNGCGITNIQKSEQFENDCKHTFCIDCSIIFTKQSCQDKIPCSYFGCTYKINYRNLNEYLKQQQIKFECSKCQLKIYRENLYCNEPCAHYLCIPCLDELLLKNKNNNTDQYLCPIHECNEVLNQPQFSQFLCENQSKLLKNQSNIQYQLQEQQNIYQVNQMQPIFQIKEYFNEDKNMVIMRNYQEEKQIQNQNQCSLCFKDFSSNQQLKKLDCLIHQIGDCCFSKYQQCPLCAKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P474</accession>
    <name>A0A8S1P474_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1050058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000108">
        <property type="protein sequence ID" value="CAD8097832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P474"/>
    <dbReference type="OMA" id="QIAKCIL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8097832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="312" mass="36720" checksum="FE21BCB60C008723" modified="2022-10-12" version="1">MSILNCLDIAQQIAKCILQKRYKNKVDMNFNLECNLIRKRWNSNGENNLAIQFYLKKQLKPYLVEEWNILCNLQQINENSQQFFEKCIELTQLTPLQQKQDNEIDHIIHLNENKSTDWIDPNLIATLQIQLSNKMTVNVAYLQNFDPIEQMEKESVNEFKSFRRDRFLSDDIHTQNETQIRKTSFHDLDGLRKYKKNQAIITQAIHRKNDSLSSNSSKNKSTQLLTIINKKDDVEILSSAEDAQKIIDQDENGDYEIQMIIDEQPMDKGQTVIIKSISQMHPVTDLILKIREKKHHYSQQLNETLSFIKSYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P797</accession>
    <name>A0A8S1P797_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1070205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000110">
        <property type="protein sequence ID" value="CAD8098763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P797"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8098763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="119" mass="14309" checksum="C9EC36198A28BF9F" modified="2022-10-12" version="1">MYRYIRNRIVQYSMNAKLEIHKADQNLVQTLQINQNVITIQQKKNAQFILVINHVNGMEFLVYLKIGVIVHIQLNYLQIKVIIQIIKFLIFHLIRNCSPTSYLNFFLFLMFLDYFNLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1P7N6</accession>
    <name>A0A8S1P7N6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="18">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PPRIM_AZ9-3.1.T1090001</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 18">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="18"/>
    <dbReference type="EMBL" id="CAJJDM010000112">
        <property type="protein sequence ID" value="CAD8099147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1P7N6"/>
    <dbReference type="OMA" id="FAIMTCH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000180">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000009">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF5">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 17 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="314"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="856"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="883"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="934"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="988"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1021"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="68"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="20">
        <location>
            <begin position="821"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="15">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="509"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="551"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="575"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="606"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="686"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="687"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="688"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="769"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="775"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="795"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="798"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="799"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="799"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAD8099147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1069" mass="122435" checksum="2D47F2ABD769D7B2" modified="2022-10-12" version="1">MESEQKENSQPLLKQRYEPFSIEETKENVEIEQENKFDQFKDFVFGIKIQKSQRDVYLDGKVNPINKQNNFIKNSKYNAFTFLPLVLFEQFHQFINLFYLGLTITQCISFLKVGFLIDYLGPLALVVAISLLKELYDDIQRHMRDHQINNYEYGLITRNGIQKISSGKIKVGQIVEVKCNERIPADLLVLYADDEQGNVFIRTDQLDGETDWKLRKAVKATQNLIKNGADPSSLISQNSYATCQEPIANIYQFSGLFNYRYQKESLSLEHVMWANTVLATGRIYGLVILNGKETRMAMNSKTPKTKFGILDDEVNWLSFLLFVMMSIMAGIVTAFSGQPATASVVTVAYIRYLVLLSNIIPISMRVNLEFAKLVYSYKINIDPQIEGTITRNSNIPESLGRIQYLLSDKTGTLTQNDMIFKKLSLASAQFSEEDKDEMKKILIEQCKLNEYPLQDVVNFLNKNNSKLRLKRERDQVFRDLMFALCLCHNVTPIYDGENPKTFQASSPDEIALVKIAEEIGIELISRDQIQITIKDCIERKQKYEILNNFPFSSETKRMGIILYNREANRYIFYVKGADAVMKQLVQEHQRGFIDEECENLAREGFRTLVITQKLLTKSEYENWKCKFDQANEQIENRDTEIQKAISLLEKDMEFLGITGVEDKLQEEVCSTLENVRNAGINVWMLTGDKIETAICIAISSGIKSPTQQIFLIKETSDPEKFQQQINTFGIETNNNLLVIDGTSLNTALTYFEELFFTIATKSQSVVCCRCSPTQKAQVTECIKFYTKKVVACIGDGGNDVGMIQSADVGIGIEGKEGKQAALASDFSIVKFKYLNVLLLWHGRLSYKRSALLSQFVIHRGLIISVIQAVFMFVFYFLSISIFSGGLLLGYSTIYTMFPVFSIIFDEDVDIKIALQYPPLYRSLQKGRDLNAKTFMVWVWKSIFQGVIIMILALVMFKNIFLEIETVAFTALIFNQYALTLSELHSLHIVMIISNVASAVIYILSIMWFPNQLLVSAMTFQFFFYVIVIVLVSWSPCFIAQKILKKYDPSDYEKIMANVKRELINTKIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PA38</accession>
    <name>A0A8S1PA38_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1110057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000114">
        <property type="protein sequence ID" value="CAD8099962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PA38"/>
    <dbReference type="OMA" id="KNPTIFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8099962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="312" mass="36999" checksum="EE5CA5CBA9B41443" modified="2022-10-12" version="1">MVKQQKKSNFTVIPPQQDFQEQEIELLQEDLNDDLIKTLSKANTNSIATIKEKEKKVVEFKSRVLELVEIFVSNTKNPTIFIERIYPFIKMISDFKNPQKIQYINRVCKLIHQIIQKGDLGEHIDNMKQCCETLITYLHKCQDKSINKRFFELLDLILKQISQHDKKAVSDIIVKGLVLMRKNSNIQMQNIRKLIFNYQFTLIPVILKLCQLSEINENYERSKYNFSHLLFTLIQNEQIKKKIISKDKIKQAIIKLAQHTLENYAQQNYKRVAIFRQILLSYKSLELNELQEQIQTFQSEDQNIQHIITKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PBJ9</accession>
    <name>A0A8S1PBJ9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Auxin efflux carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPRIM_AZ9-3.1.T1120180</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in cellular auxin homeostasis by regulating auxin metabolism. Regulates intracellular auxin accumulation at the endoplasmic reticulum and thus auxin availability for nuclear auxin signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the auxin efflux carrier (TC 2.A.69.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PBJ9"/>
    <dbReference type="OMA" id="GETSIIM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045033">
        <property type="entry name" value="PILS1/3/4/5/7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31651">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31651:SF33">
        <property type="entry name" value="PROTEIN PIN-LIKES 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="341"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8100567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="41931" checksum="39DA56CDBF672F3A" modified="2022-10-12" version="1">MIILTIIEAVVSATFGALVVASSGALLTHQKIFNNEFTMTLSKVVERMFLPAMIFTNFLKTVTLDSLFGLIPTILTTLFCILCGYLIGVVSNKYWIKKENLNSIIVLSAANPHTINIQLQIAYGLTTYFSKITGQSEKETESKLVTIIIIQTVIVNAFRWSIGKRIMQQHKVTEQQSEVELLTLKIAQPQQQLQINQNKKQESSFWNPPLISVIVSLLCLPIQNQLINDTFIHRALFLPLQTISRACPPSVLMILGSNLYLIYFKNTQKKENLITILLIVANRLILLPMIGLSTVLFLNWFGIMTDICELFILFITFCTPSAITILVMAKQYQQQLEDVVSLILFYGYILCIITLPVWMTIYLAIFQPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PC23</accession>
    <name>A0A8S1PC23_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1120184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000115">
        <property type="protein sequence ID" value="CAD8100575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PC23"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8100575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="139" mass="16478" checksum="A495BCDB012B5113" modified="2022-10-12" version="1">MFNNVILDEEDHLSVEHQVDYQRIQMARIFQKDQLHHKFESEEQTCFSHYKFYPQNYISIIYIYQSKQSIQDQKLHKFSFYCPRKTTYTEVQSLISSIFKVLISSGSSLESPQKPSLRYQKRGGKSRNSSVPSFTSFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PD31</accession>
    <name>A0A8S1PD31_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1130045</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000116">
        <property type="protein sequence ID" value="CAD8100681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PD31"/>
    <dbReference type="OMA" id="QTFAIIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="197"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="513"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8100681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="646" mass="74941" checksum="73BA41957CD17D3A" modified="2022-10-12" version="1">MRKSKEDKFQQSFAQVSTGGGQIQQTFAIIQESRRSMPMINSLKLIDDEPWLDYKWLSYEKYLVMGRDLKLKFYSFDLKILDKSREQIEDQEEIVNQLLDQGVKFISKLDNSQQSLLSQKGVVTLRQVLENQLQTQINSDYYYQTFQMQGHTLDIQKYLINVFCLMGRKQMYYVMHFQLVPKLTIEINTNIFDMCKSLSHELGTNLNCIQSLSEIALQKSSITQEIKDNIIQPILTNSQQLNLIISNIRDYSSIETDQFQLSLSTFNILDQIQVIVDFFQLTCQQKGIELILPTESFPIFNDRDRFRQVIFQLVSNAVRFTITGTIQIFIKKKEFAYEIIVKDTGIGMAYQEENNLRRILEQRIMLRVSENSIGSSLGLFISNLLVKKLNADEDCKIQFTSSKSGSCFYFLIKNMSEFKDNVEKLQSKFHRTIVKFQTINSYYENINDVQIKFGQATFKSIFSGQSNLDDGNITVYSKEFDENKQTLQPIQSIVPQLQDTIQAYSLKSTCCSRVLIVDDEYFNIMALQMLMSQYSALSDKAYNGMEALEQINNKLIKPCPKCQNCWYQLIFLDINMPIMGGIETVRVIKRMMQNKIIKKVYVIANTGFSDLETKEKAYDAGIDYFMTKPLNMATFRNIASKIFPMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PF46</accession>
    <name>A0A8S1PF46_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1160127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000119">
        <property type="protein sequence ID" value="CAD8101776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PF46"/>
    <dbReference type="OMA" id="QMKNEAI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8101776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="222" mass="25863" checksum="923832649810F61C" modified="2022-10-12" version="1">MNLQISQKSLSNKDTELKDLIKKTEKLLETTSLAVKEKDQLQDYYKSIRNDHEQLKTQFDDLQKSNQSILNECTILKKSLNDSVESSQQKIKKDDQKLQQDLLQKNKLIRQLSEDGKILGQYLKDISSQIQQIPSDQIPQNLQILQRELQFKESFINSKLNNMHLAQIDLVKSNNANNKDKKSRKLMTSHNQLIDQMQTFQVNSDLIAMMIVQSEVIEKFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PH08</accession>
    <name>A0A8S1PH08_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPRIM_AZ9-3.1.T1170183</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000120">
        <property type="protein sequence ID" value="CAD8102209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PH08"/>
    <dbReference type="OMA" id="NAHIMED"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016212">
        <property type="term" value="F:kynurenine-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051326">
        <property type="entry name" value="Kynurenine-oxoglutarate_AT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807:SF20">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="5">
        <location>
            <begin position="2"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8102209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="165" mass="18918" checksum="A14EB7E62CA47FCC" modified="2022-10-12" version="1">MFSATGLRVGFAIGNEDIIKGMKAAQTYHIFCLNPVNQTATARCLDYTADGLYFYSLRNLYQQQATKLLKGLIDSRLNFNYWVPQGGYFVVTDISNIDIPNKYFIQNDVKVTRDFAFAHYMINEFGVVCIPCSPYYENKETGQNLVRWAFCKTDETISEAINRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PIK9</accession>
    <name>A0A8S1PIK9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitinyl hydrolase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1190047</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000122">
        <property type="protein sequence ID" value="CAD8102721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PIK9"/>
    <dbReference type="OMA" id="FFCYMHS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000150">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF827">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUSP" evidence="3">
        <location>
            <begin position="54"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="2">
        <location>
            <begin position="188"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8102721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="55755" checksum="289BE68D75158110" modified="2022-10-12" version="1">MQQFRDNDNNSENLNEEDNSSINQHVPQFPPKKAPSFSMEGPQILGLLLQFRCAQGNARRELECQLIKRLVNIKKNYEPICYRLNGTWALKYTNYLYENSNTLPGPIDNTPLLQIKEPIRNRDYFVLTDTVWKFLLEEYGGGPELLEEKVPPSPVSAISSTTDRAKSADISMMSSISQQEIPSEIPIKGMKNELFFCYMHSILQCLMCIQELNHFILNSINRHNGQKMLYCQGYKEMLLQMRDAQSDYIKINNLRTTICKKFNPKYQHDAQEFLLYLISMMEDEIIEVNKENEKKQQPKLENVVKKYLKGQIVSELICKNCKHKSVITEEFLTLSLALTKVSTVNQSLEEFLKDELIQDYQCDNCHKKKIAIKKTRITNLPRYLILHLKRFKFFPKQTKITQQIKFSIESNFCNTEYKLIGVIVHSGSLEQGHYYSYGRRQHKWWLFNDQRIKQANKIDVQQQQSYILFYQQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKB4</accession>
    <name>A0A8S1PKB4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1210172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKB4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8103687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="133" mass="16096" checksum="FBDCB52621E851A9" modified="2022-10-12" version="1">MTSLKNNFQLQKEKVRAFQDLLKKVDWKLHDHLVNQKMDFSILYVKWFMILFAQDFHIDDSLRIWDCLFCQKNNREEFLQYLAISFLIQLREDLIIGDFGQILLILQNLEKQAINLSEVIQRAYLLQKEQKKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PKU3</accession>
    <name>A0A8S1PKU3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T1210163</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000124">
        <property type="protein sequence ID" value="CAD8103669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PKU3"/>
    <dbReference type="OMA" id="MTNVDKV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035796838" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8103669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="783" mass="91609" checksum="FDA10F1DF7746F90" modified="2022-10-12" version="1">MKQIVILFVLILLTTASSDLLKSISKFADDGDNSSKFAKSKVSPFSEEESEDYDNEDMNTSNKISQQDEDDEEVDVMAQLKSYQQKLSTNKDVKPPQLQKKQVQVISESESEYDDEDDSGNMWSRGISKKLAQTESKPLVLPLQTAKEQIKNDFNQFTSLKMPEVQQRPQETKIQETNQKVQEEQGKDHSADFEKMKQAQLEKYSKRFHLDEPKESKSMYEDDSLKEFMPKINFQPINIQETKTQQQVQFIPEKKQQNKQDEPESESDEEDDIVQINQKNSSSNKNNNKISNDLQSTSDAFDFFNQNKKQQKSNKKSQKIEQSEDEDSVNEQEQNNDDEDEIYVQQVKKNKKQKEDREEQKRKQKELQQQQEEEEEQRRIILKKQKELEELKQQQEEEERLQELSKKQKVKRDIDDDQIPQKKKSKKQKKQLSLEEFQQSMKLDEEQSQLTSFAQKEAKEKSILEQKTFTLVKETPQQIQEQKVFKLQAIPKKQQQFIQEEESESESESDNDEEFDAPKSINCFESKNKFIQQICQLCQTKVAYQVNQQNWQKIGINEGFRYLLKIEFRQSFEPLIEFTFKSIGEIGSYYLSFKDHTVCLSAENLSKITNVDKVLKHFTGNQLADYNSPIDIPTKSQPINFLLSAEYDSLLNGFSCRRFQIAIKTDLKYKLSKKNDINVKQIIFYKNKLKLKYIGLIQINGVVHQASFDQEEGGEVTNLIVTQLNLPKDQGIITSLVDKYIDETFVANLLKKSEQMMADLQIQTHIKNDDIGLTFNLRKRKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMA3</accession>
    <name>A0A8S1PMA3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1220170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000125">
        <property type="protein sequence ID" value="CAD8104059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMA3"/>
    <dbReference type="OMA" id="EFQKRHS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8104059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="103" mass="12567" checksum="07D6714841D337AF" modified="2022-10-12" version="1">MHFQEFQKRHSRSKSLALPKIEIIKKTEPCPIIKKKEEQSFDKQRKIIYCSYQRDLKQMKRSFEISQKLDQDDHRIMKQKTLDIKPKQKVTQKHDDNTFLTYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PMU1</accession>
    <name>A0A8S1PMU1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1240101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000127">
        <property type="protein sequence ID" value="CAD8104627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PMU1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="172" mass="20524" checksum="BC5E62A3395E64D4" modified="2022-10-12" version="1">METDLCNVIELVLAIILLNMFYYIKGLQKYMIKEETFQPNEKSKLIITVAIAFYAFCYIFTINRDDQQQGCIIPLIVVLTFLFQYIMLQYCIFLFKTLNQGLFIARPRYNINIRKSFSIIIVSTLSLELFMSIIFQDKYFWLVFGVPMMIYLLLIRMFGYFWVINKIINLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PNJ5</accession>
    <name>A0A8S1PNJ5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1250140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000128">
        <property type="protein sequence ID" value="CAD8104927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PNJ5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8104927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="32308" checksum="13FE2ED57736C347" modified="2022-10-12" version="1">MQLRSGNFNQPPKRQYISPYQIFYQEQLKILLSQGIDINQVSCQIQQAWSDMSSQLHQYYEDQFQQCEEKFQEQLIYFYGGNHQHINQLNELKNIPSKPIRPLTPQFEYIMNNRYKFSSKNINWKQSFSLLMIEYYKQSQNVREQLEFDFERKMKDYQEEIEKWNDKYAEKYKSLKNNTIEIYKKQKTENDFEDQELNISRPIRKLIINKKEQYTQTNSGFKNVKKNHMNVEDFGLSVIYTNIMQMAMKYQQFRQKQTKKARKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PP57</accession>
    <name>A0A8S1PP57_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1260084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="MYSASAC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035912671" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="2469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8105157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2469" mass="286928" checksum="EDA5A16E97B867D0" modified="2022-10-12" version="1">MISIITKILIVYFFFIGFCQDICNQYLSYSQCIGSIQSQCKWDEARNNCHYTNDYMQGCSKFLNVMACQKQLGTPLGQFANCRFIQFCENIPDVQTEKCYNNLSKYGCLIVQNPIQICIWKNKQCYLLETQQQLRLIQDDFEQMMYSASACSLIEHYLVIHSSLLWNLISYVPDLKLEAFEQQQRDLNIEVTDKIYYDDPNLVQMQNNYLMKNGNYAWYKLGLSLQYIKTNLQISNRFREGCIALEILDDNDFLQIFSLQNEIKGVNHIYCKYLNQNPMIIERYIFIDNKCVIFNTNELLKQEITEQYKINCKQLDKYQCIFFNSKEQNCKIEDDFKEYQCIEDYNQYETDCIKGFCTIKQCQKLDNHYIDLKTNMCVNTCTTISNMIECLNQDCKYLGVKSIEQQIICAPPNICNQIGLTKSNCLLLKAKCNWDNNKNKCYELKDNEISLMKCKDALSIHVCTFILLSDQMCYWSKTIMKCINILDQPLLMAHKIFIDRDLNENDEQQVSLVCNMNLCKYNSLIATEFDEGKRICLFDQFKFSNNLHLINKYHCLNNLKGYYQWNQQMQICEKYEQSQILNKQCLELVDVNERFCQYGKLSSKSFLCIYDQITTSCIEKTEDEVNELGCFGNGLSEEKCLNKNVDGQFCTYSDGLCIEISIITINHQQCSSILNVNQQVCRMYFVNQILCKYNELTHSCNQLSITDTIEYNYNLNRYACQTVTDTYTYFDEQINRCIQLDSNDNIYFMKLKCEDNYVNKMTCLKIVTDRQICIWNTFINKCRNYVDNFPSCSFFEQGSSKTCSSLMNKNKIQQTMENYCIYQNNQCKSNIESQIDCGDNEIMNIHRIDQDGQFVQMCAFIDYQCRTLVDQSMDTEIILNTITCEQANQKSCKQIKTPGQYCYVSSTEDINNITLNKGCRNQKQDRSWCYYLNYLFSDINRLNPNICSQASDECVYDSIEGCIYPPDYTSNYDCEHPGLSQQVCVGYTNERRCAFINNICQYISENQIFIDDCSLLNQFACSLSPFIQCIWNEYQRICESMNLDYIQSITSNYFCSSNQNDIFILSGETECIEIKLDEYNLYTCDTPGLNKFGCHSIPKQYCQYLNNKCQYYSPVLCEDYSFECESNDTNDQQCVFQDGQCFAITNTLFDCDSFEKTNYLFCLQYPYCIYKDSKCQQIIEYQQYPNCSSINDVTKCTVQNNQHQCQYQQLCQSLDLVLDKCPSLIGYYSKNACKHFQDCQYGYTKSGYGFCFQGQNILNLTCEQLPQNLCIEDLTHLNSDLKCFLDDNDTCKNIQNYTNLTCNNLSEYKFSYAACMHFGNIDCKYSFQDNKCKLVNEYFSTICQGSSKLQCDLIESICYFNDSICTTSGNDDQINKYGCIHQNGYYHYYQGNCKSLESTNYQQSCHNLSKDACLSKLTKEIHCRWINEKCKTVLKQENKEIQSCQDLNQRVCQEIALSNIQCQWNSEIESCQTITVSQFDCTLADQINNTSKSLCSNQINNYCVRHIDGTKCSIITIKLNSCIHYGLNFNACLELTTIPCIWKQYNQGGYCENANLYSSQCSDLLNEKACMNLKNIGQYCMWDINQKRCQHQEITTCSSANHLNGILPCKAVNLELCQYNELINECQKINVIPTNCSEFYNKLTCIQSTKGCVWNLNSCKQQIIQKCELFLNKYVCLNNQQVGCKWIDNKCESFESISNKIYCVNLPKGLNQFACRSNAIDPCNLNYHQEICISTQSDSQNFDDYFSVSQLLDIQTLDYPQPLLFNQCEYFQTKYDCINSRKKDEPCIWISQCQKVTNFDILKCSDSLNIWGCLKITNTNELCFWNHKCLYWTQDNPIMSNVNINVCINHSQSSIYFDYSCQIKDILLIDCKSIGISKITCLSISNKSCQWLEQFNQCQEFVQDNKQNKCSDYQLISPHVCQMISDQICIHDKNTFSCVETDNNNYISGLSKLACLQNNQKPMYWNESNNCEELKITIRCDSQLKVNSITCQSIPEIPCLYNNKLNQCISQFNVWSLKCDTAGLNLKGCSMVQQEPCIFMNNKCQKFIDYQSNCIMINNVNALACASIIDQNCIYDSIRLKCDIPIYQSDQCNIPGKNKKFCETNSLCNWNQENLNCKCIEFQSEICKFDKINECKRNINCYFNGNQCIRKQCYHLQEQECNSILDDKNCYLTIFNDCQSASKCEDIIDSKLPCSSFSINSNQCIQAGNQCVSQNNLDLYCLYSDCSNPKCIYEFGICKQKTCSYYSIEDCNKIGDCYLDQNNICQILTSCSQINNYEESPMNICNQLTVQGFRCNWQKMGILDTTEVCTNQPCQLYGSSKKLCHGNEINGYSCILSSSLICQQCEQIIEKCQCENQQQICSYVNGKCVSILCSSFLTKIECLQAQDRCYWSTQMIEDNTIVEQCVIECIKIIDSEECTARKQECYYEPLQMKCLKGQKEIIDLSTDISIEEFYSNILSLLIFIFMLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PQB2</accession>
    <name>A0A8S1PQB2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sm protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPRIM_AZ9-3.1.T1260115</name>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T1580028</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the snRNP SmB/SmN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000129">
        <property type="protein sequence ID" value="CAD8105212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000163">
        <property type="protein sequence ID" value="CAD8114104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PQB2"/>
    <dbReference type="OMA" id="HDERIMI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070990">
        <property type="term" value="F:snRNP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01717">
        <property type="entry name" value="Sm_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050914">
        <property type="entry name" value="snRNP_SmB/NAA38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B AND N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="2"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="76"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8105212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8114104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="138" mass="15294" checksum="72C79913D2802304" modified="2022-10-12" version="1">MSQKSKFFQWIDYRVRITLGDGRMLVGTFLAFDKHLNVILSECEEYRIRKQGIHEIETKRTLGMIIVRGDNIISLSAEAPPHQPPKKQELQPGPGKAQPITRQGVTGAPNIGLISQPKGMGLPGQQTMMPAGMSMPPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PSM0</accession>
    <name>A0A8S1PSM0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Armadillo-type fold</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1290042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000132">
        <property type="protein sequence ID" value="CAD8106012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="ATYANIF"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46043">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46043:SF13">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8106012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2725" mass="319325" checksum="CFDA22BBEFB712FE" modified="2022-10-12" version="1">MKKQSEEIVNEIRKRQPDKPLSLTELAMIREIQYMYLKKEKCVLDLIIQYCCDQRFQIRVCEKAFLMTTIVPLLHSENPVDIIKSGTIIIACLSENQETHKRIIKSESLQSLTALIKKKLHYEITKYALQTIANLAKVAANFRKILEQNFLDDTQVLEQVLPNQSQLYVLQIIYEMTYQQHTYLDQMCKLMLKKRFIKKIMRSIVSADVDVATQAILIINQLIINSKKIDRQQNIDNKNQENLESSPQIDNNNNNKNLKRLLKLKLPPKLLLTYKLFQGHENSIKITKAIMQTLENLLKIKDFQQQLVMDSYYILCLQMFDTNVYELNYYGISCLAYMSELVESHDKLVEKKILELMYQILKDCKDQKIKQQAFKIISCISLNPKYLQNLINMGMIDLLVNSLNSDEKACKIYSILSLSNLSCSQNFHKYIGNINIKLLIHILELSDPSNHAVLRAAANTLANISIDHTYQQNFLKDPEKTIILKLIYSATDIILIKSIIIIFTNISTNSNLITDLAQQDILNCLFLLYQKDYEELRGYLSRCLSALSVVPECKKLILQKGFMKSLVNSIYKSNRETKRMILLSIMMMLSINEEFQKQFINEQGTECLLYLMSQSDNFHAYIAAKSFVLISRMESSLSQVARKSICESVAKYSLTRERRILKEGLRFFVNIIIHKRPKAFIINHLCFLAHEVLKGDEQDAQQLGMFAFMLLSEQQIYHETVMAHDHLLQTIANKVFKSNNTNDLKPQNATFLSIAIMNLSLNLNNLEKLLNFKFIKVMRDICVMLTNNQEKDPEFDFRTYLFTFIRQLLKIPNLQEKLPEIFKQGATQIIQELILSNKSYFFRSLLESINILISMSDKYPSDILNVIVKLADPDYEMDDEESLPLRVYVLNRMSYNPHSLEYFKQSKIVPFLSEKMQQDLSFLNISQYFCSLLANISKENEILNQIFEAQILTSIFKLRQQKHNIKLTDIIRLLAHLSSHPSFKPKIFSDEIYANIFDHLQQVTFSEKQDPQICYPVLICILNMTKSDYGLIKAIARSDILNFVKVYIKEQKGPQHITILCFLTLSNMLIDPDILKKEKDIPSLVNQLRQSIQENEDQLDPTQPDYVVVSFLKVMHNMVLQHYSEIEDNLIMIECLQDIQKMFIKFTSQEILGLILSIFCVMVESQKTLQTFKSNYPVMIKIFDLLHHNYEKLEQQEQRFRSSLLLLLANLAYHEHSFDFFETFLNIEDFMIKYRTAYLNLTQAPDRELLLTILVNLTYIKEIQLIMPKYEKTLQLIKEIFLSEHDESTTKRIVQLLANLSLNQDLHTWIASQDILKRLHKLFFESGTSKVLKEQIQILLANTTFTEEVHEVLINNEAIRIFEQIVNKKESIQQDTSHQQVVSLVNLGLNPRTYALIENQLNMVNLLSLLDECEKPLQIRLLKSSLEYVIDNSSQNLSNKKEILQQIIANINFLLESKSKFLVCKIDPLWQMILSTELINTVRFDRERLIGNFITIIIFFDMLEVKCNYYSHLCELADRIPHILDFQSMQMHIENLFIFNQNSLNNLKELIQQVKQKQQVEPLLISKYEQLVNQFLRLLSNVAFAQKTQVGLQQFMQKKEVEDHLLEICQFSHQQAPNLILQSLATYANIFQIDSRNYQSVFEIIAKNYKQQIKQHPLYQTFLIGVIVGVMKNRKYLLFEDGKQDIGDLNDSIQSYQKNALYSIQQQQTNNFFQKRVSSQQTFISIRSQDSEYPVNKDMSLIISVLLYVLKKGHPPTYQSAPPLLLQCLTNIAYHQENFKFLFSTKLPEYLFTYICQKENIQADGYVHALTAFINISTNKEFFLRIQAEEVYKYVVKLNQVSKPLVIQYSSSIFMQMFEYSTENDENQHLVEFTLDFMVNEVLSYVTFESDHMDLFLAFLERLLKIQKLKYKIAKIESLFQKFQKTLETQDLTNDKKFDKYWRIWNIYNFLITSKECLNYLVKYGYFSLLKSKLSYFTSSQFDQCLSYNTQAEYQEKHRNVLKQFQLFSIVLSSIEKIFKNDVNLKLQPPIQKLIYSQRNEKVSTDIIRINDNEALKISFYVPFLIIEKILLSEETYQNQNNILIVVEVLNQIALILTRMDSNGDMTLKFLIEILKRTLKTQVEDKTLFYSNVVNISIKLFEKFGVDISGPIEEILVLFIQHWQGSEFRFMNSEIYQNFLRLCVLFKFKDCQQDKPCNLANSVLQIMNINQTTFREHETQLLFQMFLKKLIAEVDPSESQELEETISESIDFLLDYIQKQSANIKAQLEMGDKSVTEYQKTLQQQNFTMSIEILTILVSFTSFQEKILDLELLSSTVQCGLLIQELLNFFENKEQQSEDLKTAINQLQSFLKHISIFLSYTSFLIKFHDELQSQPCIQFIFENMLKRKKQTKIFCFNIIVNLIESQGPNFIFEVLQSIEEQIDKINANNSLQKYSLDAIVSLTEEIYQKIQHYKTIAPNEEAFYQLSDDIGLLLTKILIGKKHSFSLNGSAEILMNSKLQQVQIRTLFLMIKKFINCDNDIAIRDKSIQLSFDISKQIYGMLSSMEQIDCNNETIQNQQFQSKFINIKYTWDQVRKNQTYQHLEDMFFWEQLELSDYQFKLIFLIICFENITCRFRGEQNNNKIALPNSAIMVNDIFLLIDPNLDTFLENYNLQNQLNFLLVYYAASISYSSLGNRTRKIEFQQLAERDMSFRIRLNTFVRVLSDSKINILANFADLIRGPIQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PX10</accession>
    <name>A0A8S1PX10_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPRIM_AZ9-3.1.T1340067</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="EGGRKHH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000068">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000009">
        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000117">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000300">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000095">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF62">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN 60E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="393"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="547"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="596"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="629"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="686"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="774"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="804"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="966"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="997"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1086"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1166"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1395"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1435"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1469"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1514"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1628"/>
            <end position="1653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1711"/>
            <end position="1729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1741"/>
            <end position="1766"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1778"/>
            <end position="1806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1827"/>
            <end position="1860"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1931"/>
            <end position="1958"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="1975"/>
            <end position="2010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="436"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="847"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2135"/>
            <end position="2170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="454"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="865"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="2147"/>
            <end position="2160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8107601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="2170" mass="252062" checksum="80F5B79D016591A1" modified="2022-10-12" version="1">MSQNEENNKISEESEQKDDSEEDEAEDKENQQRQENFGKDEHDVVQVHKSPVQVKRQVLKRKSTMKAEDMFGMNFFKFQYYVNQEELGIRKKNQNSQTGDRLEEHNFYMHEKKYSFGRDSLFILKESYIIRKYCVWIVTWHWFDKVITLIIVLNTISLAMQNYDFRVNGTSNQSELTYVRNQMEYFFTSVFLLEFILKILAMGFLFEKHTYLRDGWNVLDFAVVGTSILSLVGSFNLSAIRTIRILRPLRSIKSVPGLRILVASLLDSLPNLGNVLVFLLYLLVIFGILGLQLFAGAYENRCRFTQFPVNGTWLADPLITHLCSSDSDCPENEFCGNPINYNLPNQDNPIPELNYSYTTFDNILTATFTIFQALTTEGWSTIVTQLIDAVNPILVYIYFNLLIIIGSFFTINLILAVINDSFLKNQSERRQELLKEEQQRKQQRKEKKQKERQNAQIHPTQNIADLVPSDDSDSDEEAQKKKEEIIKQVLKPTKFQGRAEQLKILQDQFNRKVKKEKQQMNFFDPSLYTLDVNDDKGTNFDTKFKQFFLRFIESLAFTIIYWLWIDILNHNQKXDFRVNGTSNQSELTYVRNQMEYFFTSVFLLEFILKILAMGFLFEKHTYLRDGWNVLDFAVVGTSILSLVGSFNLSAIRTIRILRPLRSIKSVPGLRILVASLLDSLPNLGNVLVFLLYLLVIFGILGLQLFAGAYENRCRFTQFPVNGTWLADPLITHLCSSDSDCPENEFCGNPINYNLPNQDNPIPELNYSYTTFDNILTATFTIFQALTTEGWSTIVTQLIDAVNPILVYIYFNLLIIIGSFFTINLILAVINDSFLKNQSERRQELLKEEQQRKQQRKEKKQKERQNAQIHPTQNIADLVPSDDSDSDEEAQKKKEEIIKQVLKPTKFQGRAEQLKILQDQFNRKVKKEKQQMNFFDPSLYTLDVNDDKGTNFDTKFKQFFLRFIESLAFTIIVNCIIAANTVVLAMDRYPQPQSEIIILSYLNLAFTLFFVLEMILKFFALGIKEYFKDYMNIFDASIVAISLVEQVLDFAQISVSGGSITAVSAFRTLRVFRIFKLARSWNSLREILIAIATTLEAISFFTMLLLLFMVIASLVGMELFAYNIPNVRLNFNTFFDAMITIFALLTSESWNIQTYIYMYKSDQWWPLIFFISVVIMGNLILLKLFIAILIYNFGQKSIETEKKLQDKMRKLNFHQLVEQFKSSIKIYDPEEKIKHKSPKPEEVHPETQKIIKINQNYIGQHQNSDVACFFLAKYDQARFEVRKETKQKKLIKRFFDTAEIVDPEEISDDSSGDEEYIKQMEQKIQFQSQGPLTFQDGYQTQVSLHQTQSLSDKKTSVFSKHETSSGQDNIEGVLIVGTSLFIFEQSSKIRRFAFRFINKSQVVGFIILMILVSSVLLALDDPLEENNSEVLKEIDLVVTIIFTAESLLKILAYGFLFNGEYSYMRDYANVLDFLVIIFSWVSIFSEANLQIFKILRIFRVLRPLRLVSRSDSLKIAINALILSLSKIFNLILVSLVFYGMFGIFGTNFFKGAYYSCDINKIGEYQIIGKYDCFDYGGNWVNADYNFDNALNSISSLFVISTTEGWLEQMYQGIDSVGIDLQPIYNYNMYWSLFFIGFVIIGSFFVMNLFAGVVLDAFNSETDKLRGYFYMTLEQQEWVDIKERIYYAKPSINQRKSDQWLRAKMFLIINHKYFDSMIFILITINTILFMLQHVRQPDILNTIIQYSNLCFLCIFTTEMIIKLIALGWQYFAEPFNRFDFVVVILSIAGSILEQMSILTAFGTVISVFRSFRIVRIMRLIRSARSLKAIFETFFLTVSQLANIGGLLSIILFMFAVLGINLFPYVKWSTNGLTGSINFSTLGKAFFTLFKCSTGEYWDQVQADLVRQMTPNNVCFSENLLIDYQEYGFNGCGYWYGVVYMVSFQMIFAMIMLNLFVAIIVQGYEIIQKDEASSISTNHIQAFIDVWKLYDQTGSGLIRALELDRFLIKLPKPLGWKGLLLTQMEKRKVINQYRLPVYIQNQTVMYFFYDVLEVLSHNILTRKYGDDTELEPKKLVKATKDLIKKRLQLIEQLKNVKTCAWTSEDINSAFIIMRRMKRKLDELKEKRKNQVQIIRDLSSVQSSEQDDSPDQTQRHIQLNTQGIHSGRQIIMDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PX53</accession>
    <name>A0A8S1PX53_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1320142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000135">
        <property type="protein sequence ID" value="CAD8107089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PX53"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8107089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="28460" checksum="43E42A8EF3FF4912" modified="2022-10-12" version="1">MFDEMMDSQKIINFVISIFQHVMDLFHQIVQYVIQNIEQWIYCLTCIQLDIMILDNQNVHVFLLNSIRIPLFTITILVINVLIIIKKVVLFVLQNLILEYYKEISANLLMLIIKSKEFLSIKVTFHYYQSIRYIYETCKSQAEKCFTFPLNPLCILDSVKRCFYPEEFYDKQDSIVSKKCHFKCKSCDGKIRNNCLTCDSYAKRELINNEWKWQSDFFQIQVQECTSDNINSKTYTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1PYP2</accession>
    <name>A0A8S1PYP2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ODAD1 central coiled coil region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T1340063</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000137">
        <property type="protein sequence ID" value="CAD8107593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1PYP2"/>
    <dbReference type="OMA" id="IEETWVT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051876">
        <property type="entry name" value="ODA-DC/CCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049258">
        <property type="entry name" value="ODAD1_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21694">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21694:SF18">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21773">
        <property type="entry name" value="ODAD1_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ODAD1 central coiled coil region" evidence="3">
        <location>
            <begin position="85"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="79"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="281"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8107593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="471" mass="55788" checksum="27C48178E2D328A1" modified="2022-10-12" version="1">MFFGYNSAPNSNISQTQSILHESQVLSLQRESENYIRKVEQDKKHHYNIEETWVTTKKEWQQKKEQIKKIQHDQTLPAAKQQLNKIKNLQNALEQSILKYNESQTQNLELKKQINMLRKERHQYINIHQDLQEELLRIENETVTNEAFRNSNEIQAQKKKETIAEMKKKNDQEKEKYVEQFDRLKKEVMEEKKKHDLDNLGLKKEKAANIDTASTLKLRLKKLISNNKEKVKLIDTYWKNMKVIEDAFNQIKEASGIQDIEEIMNTFIKSEEQNYSLYNYVDILSQQIDQLQDQNQDLKKKIDSQRVENESKKRLLMATPQAERHRKKNELIIKKRQEEINTLRKQMEEIGPTLKDALIELSQTQLASDPTAHLDYKLSFNLNESSLEKYLQDLERFIDLAVAKEKIAQNQSIAQSTLLLDEIPLKEFKGSNKQFNEDELLKQESQQQQYNKLLTQKALRDMAVESLKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q1W8</accession>
    <name>A0A8S1Q1W8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1410081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q1W8"/>
    <dbReference type="OMA" id="YLTICFI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="749"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="771" mass="91197" checksum="2949E07E9E391D80" modified="2022-10-12" version="1">MYPFRANKSNSQESFFVLLVDGTTSMNAHYPIFLKCFHKVFNSIKHKLAFQFGISQDRTDSVLYDINKYLDHRESNFNKVFKQLFEILITQHIDKKYLTICFISDGVEPFIFDDFLPLVKQITSVFQIQFASIAVGKNFPTLISNQLRERLHNNGNLDFPFIYQVMDQGQIRQELDNYNNVIINPQDENKIIQEFTDAFESLKQQLIVYTGNIELNEIVYKSFIQNEDQATNIVQCNQVFLSTNPTVETKNNQVIQCSQDVEDFTQIQKGSIQQLLIHLQANNKIEDSQKEFKKLLDISTKSQRLLVQINQQKNPIKDQQQINNHPILNVMNELMNQFVQTNYVASLKNNENEYAQLQANLEKNPEKCIKKSVEYSKSFPQISQCQNIIQDQPQLQIKDIEIQPIQHIQLEQKVQKKQNIQHHQQNINEVKLNSKLIILIDSFKISELQAEDIINQKKTIFDKQEEENVLTLWWADTIQQIPKFNSEGQQIPLTQAIQQILSIIEQDQSGIKNFIIWLIISGQHKVDFLSLKQQQQQIQQQNKQIAFTTLIIENNQQNDDIKMAQLKLSLELRRQLHTNYQTHSLQVSARVKTQEFHSYLNQYFRMVKDYQNKFYRIQKKGCYLRGITLQKNAMHLLKQFINDIEHFRQQLNEDMEFIQILKQIKQKYYKMMNNFKEQEVPNEKYCLAKLNTIFELIDRINLNNHCDFLDEIFKIKLGFYDRNQKVVDIKQLNKARNIMQNNNQSQSSLITRSALALIFIYLFSWIIRNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q2W3</accession>
    <name>A0A8S1Q2W3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1410163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000145">
        <property type="protein sequence ID" value="CAD8109788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q2W3"/>
    <dbReference type="OMA" id="QMISIEA"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="214"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8109788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="409" mass="48386" checksum="EB18E6B63D09E24A" modified="2022-10-12" version="1">MQQPQQQIPNTVPLDILLAQLKNPLIVREDHQSLLKQKHHQMPEIINNNYELLADKYLLLSNALPGCSPELIVEEFEKVVQVVEKCCNQFIEKMKKVITDLQAFANYDTQVLKGYLTQATDVFQRCFYLAEKDLTNFDVDFLMENFTNPENGIQNHLLTLIFERNQNLQNVMQKVYTEQLDFAAQFRAQFEQLCNTNHFEPLNKLLNNQKQFWSNRVKKQIQTQKKLMEEQQAQQVLVQSIIQFAPQIQQFQQQQQQQQQLQQQQLLQQQYQQQSQINQSQNQQQQPPQDSSWLTKSQMISIEAGNQQLIETMLKLRAHTHELMQQHDKDSKKDHKRHCKLEGGGSFGYCWTGGYGWKGTIYEGLELSNQCPQCQVLNQDMHNTAKRLYDLEYRDKGTWWVRAWFLYKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q5L1</accession>
    <name>A0A8S1Q5L1_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1440108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000148">
        <property type="protein sequence ID" value="CAD8110537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q5L1"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8110537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="65" mass="7714" checksum="E60DCB360D22FE2B" modified="2022-10-12" version="1">MEENMKDNTLMIKNMDKEYQNGVADGRKYSGQWELGKQHGIGEYFNGQINKKGQWNYGKRLKWLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q6Z0</accession>
    <name>A0A8S1Q6Z0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T1450141</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000149">
        <property type="protein sequence ID" value="CAD8110885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q6Z0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="267"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="347"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="389"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="396"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="469"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="511"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="594"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="636"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="690"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="725"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="767"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8110885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="881" mass="97656" checksum="158371BAAFDF9D1F" modified="2022-10-12" version="1">MQAHFLEKEALADIFSQIKDVDEQIFGVILEKLRKEKVQDIIGYLSDNRNQSQLESCILQKGVDITQDDKEQKMSVVRNNIKQIIEVLRKLKDHDFNKIDYSSEENEESKLSLIRSIKKNQGIIEFLQFLVHLTSIDDILIQCGSNSFHLLVWMKINLSNKNFENIKILNTSLVGANFFRCNFSGSQFDNVDISGLNLNRAILLNCKWNNLRIPEVNKLDDHKDEVRSICFSPDGETLASGGGRSYGDGDCSIGLWDVKTGQKKAQLDGHSSCVWSVCFSPDGNTLASGSDDKSIRLWDLKTGQQKAQLNGHTSSVCFSPDGNTLAASEYLSICLWDIKSKKKQASLDDHTKTIWSVCFSPDGNTLASGSEDKSIRLWDVKTGQKKAQLDGHSSCVWSVCFSPDGNTLASGSDDKSIRLWDLKTGQQKAQLNGHTSSVCFSPDGNTLAASEYLSICLWDIKSKKKQASLDDHTKTIWSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHEGQVMSVCFSPDGNTLATASKDESICLWDAKTGEQQTKQDGYEFGAFSVCYSPDGNTLAIGEHMDICLWDVKTGKTKTTFKDFDLQVNSVCFSPDGNTLASGSNNKSIRLWDVKTGQKKAQLDGHTSFVWSVCFSPDGNTLASGSGNFWGDDFSIRLWDIKTGQLKAKFDTPFYLVKSVCFSPDGDTLASGSSDTSVYLWDIKTGQQKAKFDGHVREVNSVCFSPDGNIIASGSSDKSILLWDLKTGQQKAKLHGHASDINSVCFSPDGNTLASGSNDNSIRLWDVKTGQIIQSSDQKNKNLVNPNLDTYFTRLRISKYLYFEAFAALIFNGKFVNRQGKDLIYLFKNRGACILQDSLNILTNTIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q767</accession>
    <name>A0A8S1Q767_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1460048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8111004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q767"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF42">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE, ZINC-CONTAINING (AFU_ORTHOLOGUE AFUA_7G04530)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="151" mass="18101" checksum="3A08AFDF397CC533" modified="2022-10-12" version="1">MECDVIGLTGDQEKIIFLQMQFPNIRIINYKLYDWDKQIKNNSVDYYFDNFGEQMLEIMIKNQKDCVMWRLSNINQIIYKQLKLKGITFNQELNKIQAAFGYLFQQFQQKKLKIFQEEYNQLNDTPITLKRLFQGKNIGKIIVNLDNKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q8V0</accession>
    <name>A0A8S1Q8V0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1490035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q8V0"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="64" mass="7628" checksum="DA7C965A8B2BA077" modified="2022-10-12" version="1">MHHQQQMEESSTANFIRFTHLNDLIQMVVQYFHIDVAYKIKSSLNNQNRKAQKGLEHIIEDTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9A4</accession>
    <name>A0A8S1Q9A4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1460101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000150">
        <property type="protein sequence ID" value="CAD8111110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9A4"/>
    <dbReference type="OMA" id="HEQINIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="419" mass="49400" checksum="411E3EE10F2F4F2D" modified="2022-10-12" version="1">MDGHFELPFGILQQFIEPADDRNSQIQAFWSKSVTLFTKRVCKKSFTNKSMNIYERHCTFEGPEYFSEATQVKDFQSQRISEQIDKMISHLDAISYGKLNISQGTFYFKVDKKARCWFLFCGNLKFEDDKHLKNLPKDLYTQSQIKIPKSIDNLMSVYNSRPLQLNREHKCIRCGQLEKGSNFVEVPYHFLIDLPENPLPVEKWPLDIKKSAKQVKLSGANQEKLAYVNTISQIPMTFQKIHEQINIQNYQEFKQSQGFKYKTITVCLDCYFIMVENQERLAMKEVTSKEKTSMSRVKSKQFQRKEQNLNQFLNSTQLFKQVKSVKNFKMKEIPSQTPITKQNTIQKYVNLRLNNCKSVTPIKYSIEMPSTADYLSRNTKSTYQDLSSTYKFKNSRENYNQSLQERQQNRLIEIKDEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Q9N9</accession>
    <name>A0A8S1Q9N9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1490027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000153">
        <property type="protein sequence ID" value="CAD8111767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Q9N9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8111767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="144" mass="17814" checksum="6C5BCA6B4E30F0DA" modified="2022-10-12" version="1">MRCYYSLYDLSSIFTIVLMRKNNTLSNPIREYYLNEYEMDNQYNPYTYIQMNEIKQCQLSRQIQLMICYQELGDNETNLIYLGRLQSCNLYKIRIFSSEFLIVTINLYITQFSWYIVSKEKDEIEKQHVYKILRNRIKRLYYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QA34</accession>
    <name>A0A8S1QA34_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1520042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000157">
        <property type="protein sequence ID" value="CAD8112589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QA34"/>
    <dbReference type="OMA" id="DDQFISH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8112589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="46779" checksum="87942AE5A4B64BAF" modified="2022-10-12" version="1">MIKIIFPQLNNHTYEIKNPDYQKPMKFYFDEAIIHLQKQIPEETKICLTINDQEKDMSQDLIHLQINTSSNIKIILSIQIQITITFQTMEEKVTHVFNSFDNLFILEKLIKKTHNLVSYDLNFFYKQQQLRKDISLKALSIQNDSVINCQIMGTYSINYQVEQFIMINIFSTIDQVYQQIQNYFHLKQEFQLYFQDELLKQKQYNRDMLFYEYKIKPYSEIRLKLSDNFTIQIHIDSIKMLQHYMVSKNTKIGDLKNYLIEQCQIDKHCQYQLFIKDQILSDDYQISELTKIYKINYKQLNKIFTLKVVDKSITFYICKKELDDFKIRVIMNQNNQIQDLQNLKQLEGYKYQFYFNNQILNNHNTFKQINLNNHDIIYYEIQPVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QBC4</accession>
    <name>A0A8S1QBC4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T1500107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000155">
        <property type="protein sequence ID" value="CAD8112244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QBC4"/>
    <dbReference type="OMA" id="QEDDCEQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8112244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="283" mass="33817" checksum="4C71A0B217ED8BA0" modified="2022-10-12" version="1">MYKQQQQQQYSHPSQLIPSVCTLNTQEEDQQDYQLIQRKLQCYKDNLKIIQKNNEDDRKYLSSSSDVLQLTESQEDDCEQLKQQINFLQDELGKTKQNMMQIIEELTQQRDLLIYKIQELESTIDNLQQQKHLKNKSIHSTLLDITYEQDQTQDYGRKRTRSKYDDQNCWKRIFEELADMLKTENVIQEVHRLQQKCIKQDKFIGCVQDLVIKLTPKDIFEHSKPQLRDCWHWIKGICTEYMNLKKMQATLIVETCSQILNVQPQDIVGQIQFLIKRIIAQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF11</accession>
    <name>A0A8S1QF11_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1540070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000159">
        <property type="protein sequence ID" value="CAD8113170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF11"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="25152" checksum="B0DD82E01A1AF4FC" modified="2022-10-12" version="1">MQQPIEEIDQFILGFNNQTSNSFIQSNINQEKLLVQNTRLLQQLNEQQRMLNLHSLLTQNILQNPYPNQQTTHKNKMIKKAKSNLQNIQSNACLLDQPLQIQEIKGNVKNAYIKKITSLLSVEGDKLLDKDLQENLEEDSSSQEPSRDKYGLAGLLCKDLLNEECKNKRIRQSAKKSRLRKKVYVKLLEKKVLDLDHKIQEQKQTTKQSFEYLTNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QF83</accession>
    <name>A0A8S1QF83_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1570050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000162">
        <property type="protein sequence ID" value="CAD8113906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QF83"/>
    <dbReference type="OMA" id="NKQPTSY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8113906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="332" mass="38797" checksum="5820D16C74722815" modified="2022-10-12" version="1">MLKKYLSSYYSNEQIRSKKINLKPKLDFQGFTKAKFINQDLTQRTIDYSSPKIQLNFGSIEHQNSKIIKPQTKQTNLSLYAQQILSKNNNQSSIDNTPKKGLDLLITKRDEAYKYRSVSQTQRNQLIKRQSSEEKCKSSERIKVIDVFNQSPKHNKQPTSYQTYITSKEILKVKLKQQQQNLQSKSSKISIHINNAQQQSIKQPISSRLNESRDILSQMRRNSINTQKLNNNPDREGVITQKKKYFFNSPQNNDSIGFEHDSKSNLQVARNLKIELEKVDNENILELLLLSTQELNNKFNKKSQLIRNESEEKIPLKIRVRAGNKFPKDFFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QK55</accession>
    <name>A0A8S1QK55_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T1690016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000178">
        <property type="protein sequence ID" value="CAD8116073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QK55"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8116073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="47" mass="5679" checksum="098D07D703624208" modified="2022-10-12" version="1">MDLYMRCQSFNGQCTAIQASIIQWMKYQQIINQMEKCWFNIKLRSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1QSH9</accession>
    <name>A0A8S1QSH9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T2030017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000212">
        <property type="protein sequence ID" value="CAD8117567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1QSH9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8117567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="442" mass="52030" checksum="6533EF1958E8AD1B" modified="2022-10-12" version="1">MDEFKQQLILNFQNIPTHSNKGLLLFFQIEVLNDLINSQKINAASTLSENQEEMIFVNNTYQQLVNQFSYAKFIIGEEGNIELASNSVSQQLNIGVNKYSAFNEMVPQSLVSFHKQFMKEFISTAQNKYFLNINQSFYKYLQAQQFLLNCMNQLMATIFIIEAHTNNSFHYIIVDDRYRIKKISQALYFETLEYPTFVKMQLYNSSIFTLIPELKKEDLSLDRTLRSYSLCFPQKKQTSNHKNIIKVIFPQSQELLIRKSYITFLKQVILNQKEKMSIQQGDMDEKEIINIPLEIHNLYEQTVPNQIWQLPINSNLESERPLVFLSNCDPSELRFKNSKDQDNFNIMQNKAKSSINTEKNQISKNDQEKFDAGSQISSVAAIRRSIYFKQFQKLQMIQYSLKYFHPILRNLTPSLQSMLLLEFLILVFQLIKLQTYRNIKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMG4</accession>
    <name>A0A8S1JMG4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0040119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000001">
        <property type="protein sequence ID" value="CAD8042901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMG4"/>
    <dbReference type="OMA" id="WILFAYI"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8042901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="474" mass="53854" checksum="AE7B955CC9F56162" modified="2022-10-12" version="1">MSELVLIKEPQNNKQAIRELILPSLQTAMSFILGYLPNTISIYLIEFKEKDSVLVGALGLGILITQAVGMGIQNGIATGLETLVSQAYGANQFELCAKLYYRSLFVCTLFMIPISLFLVFSTQLMSLINSNEQLVQEAGRFNKYMVAAVYLDAIFVNTKVFLNGQNIYNYQFYTQLISCIFFFGISYFFIVQQGMGLIGCSLGWTCFEIINNILLFSFIFITKCCETTLFRFKIVYLHNTIKFLKEAIPIGSILLLEWISYDIYLILVSYQDESIISAHVLMANFASFIYQFSYGISIASTTFVGNEMGRKNIQMAKKFAKATFFIVTIFLVITLGSFTIFGKQLIEKVSHEEIVVQEIQDILYILVFFIFIDGLQAIISGLVRAVGRESSASITFIFCYLVLGEVVGYFLCYPLNLKLKGIWIGMTVGALTYDIIQFINLIWKDWNEQAELIEKKIEALHRLQIQLLSTEEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JMR2</accession>
    <name>A0A8S1JMR2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0050107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JMR2"/>
    <dbReference type="OMA" id="KENYAYY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015496">
        <property type="entry name" value="Ubiquilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677:SF3">
        <property type="entry name" value="FI07626P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677">
        <property type="entry name" value="UBIQUILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="152"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="635"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="728"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="635"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="729"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8043454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="762" mass="89106" checksum="ECB6DFB0751CB2C4" modified="2022-10-12" version="1">MIVNQIYQPVEFIYSYTIGGKQEQHLQLKNQLPQKKRNLTDEDRRKIKNEIQNEFNKKKILKKKIHKQIIIKVNHKKYLKLFNKLKARKNKVKKLSLGKKIKIIKRERIFQLNKEDDDEQVKVKDAYDKEYDKGKIKKVKKKRENEQFNEEICVKIKTLDNHNLDVRIKQSQTVSDLKNLIEIVSTIPSSRQRLLFKGRQLNNEDTLISLNIEDQCVVHLVANMPEFESSPLNRGLSTSSIDEHDNRNSFDVQENRRNSRKKVLQQRLHGFQQLPTRNSLQQNVMTLHNLLQSFNTVAEVIEQGQIFAQKDFELGQWIDFKDSFGEWVEGYIGQKQQNQVLIVQQNGEEWIGVPSNRLALFRSHTIQKTYHMSPILNNHEQQQLWTFSELLGQTAMLLSRAGNMMSRLADSVEDKSIPQNKKADMSNLITGMIEEQRRIQEEKVEKQDDVTSLKSMKSMKSDKISIDYETSSIKSMKSDISKLTTYFIGHNHNTMMAENIAEEEENSSQDMSVDNQRDVPNISKEFKENYAYYETSLLAAQLAPLSDRLGRLLVDLSPYLALSGSNINNIFQNHPNSNISNLSIITNEGSQYSTQAKQYYFQVPILLTPFELHTQSQQGTSANRIVGDNVDILNHLNNNNNGSNPRQKKTTKKEEEEKQQFVTISKHKLTQSYHLSYQQQQQQQQQQQQQFQQQQQYQQFQQQQQQIKESNFQVIEQDDTLQLQQYQSLKKESTDGQIKKKKKGKEVSFSEQINQLKKDKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQ30</accession>
    <name>A0A8S1JQ30_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPRIM_AZ9-3.1.T0050303</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQ30"/>
    <dbReference type="OMA" id="NEQYQLP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="7">
        <location>
            <begin position="15"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8043649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="100" mass="11265" checksum="19721310CAE3DCC5" modified="2022-10-12" version="1">MQQPNDDQIPLNIPIEQYQLPQQLDEFGQPVMFKCSTCGVQAVSILQHKPGTHTWLCCVTLCIFGAFLAFLPFYMKDCQDVTHVCPACSQPKGVRQFKAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQG1</accession>
    <name>A0A8S1JQG1_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0080362</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="NFMENGL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894:SF5">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE RSMI, CONSERVED SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="1098"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="740"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="303"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="548"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="757"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="799"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8044793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1281" mass="152057" checksum="48BC4FE0DF811292" modified="2022-10-12" version="1">MNKTNCFPKIQKAQSTAKLIIKTDWDQSINSSIPLTIRAEMSSNRMKTDFTNKNFSSNSDVSYLKSKHKKFQSIESIIYQDPELSQTKYDKSLQSFYSTMYRKSQNKLQFNNKIDKTTIKSQWLDFEDLLKDKNFMENGLQKYFFDILKHEIKQQPNQQAIPPNRQQALDLKEWLFFMIQRTKDNSKNLNAIQIAEEIQLIYTTCFKEIIQQVKVECIERGELLQIIWDFYIQLIDQVIKSSNQQMKGFELEMKNKMIEQQNYYNEQLHFQELLYNESKNQIKQLQFDYDRALDIDQQQKEEILQLTQEYRESLEIIDQHRKQIDELQLQIVMLKTEQVNDSKPESEKINIKVQQGQSIVSHLMNFQAKIDNNQKYRIKSPFKSQGSKSPCRSPKKNSMIFDRWEQLKQQENQLKSQIISLKYLDFDKDYQPSQSKIVQISGTDDKAIQTEDYVQDYQNNTKKEEQYINNIDSNISIQENNNTTLSDDLESKISKVFKLLGDQKLDDNQKLLLYQTLSESKEVCEQNKSLSQKLISMNKLDSMNKMELLEKEELCQELEMKLKQTVQDFISIISNSKRKLKKLASKNTQLIGTLKQIESKYEIVINDVKSIEEEESRTMNLINEVDGDENVYSIGKESQNMDDEGSLQIYEDESIKHSIKMVGSIQFQKQPIQQSYDYKKAISETNLDDQQEQFDNSFNNQINSKEEINNQKNGDYSKKQSQHVNINDENIYKETRDQIQSENSKSDYLINPNQKNQIKELDESKTLKSSTVNKNKINNLKKQKSIQKHQQQQGDRKSINQQQNHMRNSKNQLENKIENRNSKHNISKSPIGEKDEISYMNQSQKSIIESERMELDQDKNCDQRIKDVIKQYKDKQFTKYSFAHRTLNFNLLQETKQPKRFRRIYSQNTDVANNLLKQVINSKNITKTLSFLQFNKIINQILTEVSKQNEGYKIPFHVCIYDFIKNKYGFKQVAEKKIKQIYEFIIIEKEKNAKVLLISKYCNLKNEIDEVAQKLLIEAFEFFNNKIESNKNEFLITYESANEFLNDKSQLWLQANQIQQLQNQFKVQNQQYGSNKYCINYDQFIMKILDFYIFNKKDYQNILETLFKAADLDGNKLIEYQEFKTLYRAIHIQQIETENLLQMFVRNADFVDDQGDKYLTLPRFTEMAIELAIFPKDSVIKYGEGCEKIKEHWKTEKNTIKLRFLEAKQYSKVKHTFEELDNLINNDKKEIQNVLWMSYKLLNEQSLRVYLKYQTKQCLSELLPLEILQIQQQYKQLDSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JQR5</accession>
    <name>A0A8S1JQR5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0060367</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000003">
        <property type="protein sequence ID" value="CAD8044285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JQR5"/>
    <dbReference type="OMA" id="REIYNYE"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035905044" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="427"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="55860" checksum="D39C1122C7BF43B3" modified="2022-10-12" version="1">MFNLIFTFILLLCQTVNTKNKILFPQNRFQIFQQKWGSCNNSSSIQPLINNFEQPIQLPISYNNPLCLHDLFQFSLSEPMSFYYFVFNRTIEYDDLLLIIIDGKIYYITQETATDNFVLIRHQRFCSYNSIEFYLISKTKDLGLKNYLILISENQNQGEYNQVLSLSHLIIGEEYQITQYNLQTKNQKIINSILIYEGQLSFENIESVSSCLEFNIFNFYEQMKCINNEIDGKIRFVCTLDINKIKDVSQIKDLSFLILSQYTFHFYTPCFPKIPDKYIQFQTIQQISDNNYSSIANHIQELKFGQQHQININYNYTIKFRYKNQVQISLNPYESQQCLSQFEEAFITIKNSLGETVKCDLSYDYSCLSQIDCSFFIDLDFKDNVGYIFNLTAIVKRPNMNSPSYQFFLVDIVLKDSVPPLEPDYNFTFIAILLILSLSAPIICFLLSTFILNIPIYDNWLREIYNYESQVLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JS09</accession>
    <name>A0A8S1JS09_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SF-assemblin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0080346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000004">
        <property type="protein sequence ID" value="CAD8044777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JS09"/>
    <dbReference type="OMA" id="DACIRID"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8044777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="30287" checksum="02DE5E65113A1669" modified="2022-10-12" version="1">MLLQQQRKSVGQPQQQTASSHTIKMISEKWRRISQEVDNQKVSRLQQLQDKMKEIEKQALTVNNLEIKQLQDSINTLNQNVQDHLTQEYNHKTQKAYENTKKSLDKSLQQQNQQSLDMDKRISHLLDWNNLQDQISRNESQYDFALQNKLQMIADSVNQIEDILESEREQRLQSFEQIEGELEQNLFQLKNFISQESTLRRQKENKIFDMINDVHQNLSYMLQDEKKERQIMTNNLMQLMDDACIRIDRSLSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JTL9</accession>
    <name>A0A8S1JTL9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0050259</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000002">
        <property type="protein sequence ID" value="CAD8043606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JTL9"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8043606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="179" mass="21481" checksum="5B348CD39F915D36" modified="2022-10-12" version="1">MYSFNVPQIVQPICKFIFLYYTLYRFPSIFLQYLFLQPQYTFTPSLIDMYYYKHNYLFIYIGPSKYQPSAHFHLHIMMLIGFTQMKMGNGKFLIGEDAISQKICFIIVIKESSRLYLKTCSLQWIMIIWSYIIKLVIIKLGENHQLCLDKSKYNKGNGLIIMDKHGFNMKKQYKINWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1JU81</accession>
    <name>A0A8S1JU81_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0090175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000006">
        <property type="protein sequence ID" value="CAD8045149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1JU81"/>
    <dbReference type="OMA" id="EQMQIET"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8045149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="166" mass="19783" checksum="A7D9C33D0C1AC875" modified="2022-10-12" version="1">MFGKNIYQDDVPNFPEISEDYFEIKDEKTINELMNLYNSSKILIPVNGSVSNEFREKQLGLLSLFDLLDNQLKSNQCKPDIYEHNEIDCSINTPYQNFDYYNLSNIDYDQNEAQKEAAKFLTNNKNELQEKFEKMQIETKHNNKQIQHEKLTKKMELLSFQDQEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3D2</accession>
    <name>A0A8S1K3D2_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0120373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000009">
        <property type="protein sequence ID" value="CAD8048222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3D2"/>
    <dbReference type="OMA" id="NQCEINH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8048222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="35946" checksum="BB74B5C636AD8E9C" modified="2022-10-12" version="1">MSHSSSRIVVQMLTLNSLLQIYENQPFEFNDSNLMMQHPQLTSIIPTNQLINLTVGILILFLIDWKNKLNIITSLFFGLSVIMQNFKQINNQCSYLLPKTLNIIQIYLQLLIIIKYIYFQTKKIYFSIKSLENNYKPTITISNKLHSKKKITKIPKKVEKQKQKQENTITNEIQAKIVQQQMNIEQTQIKDVQIQEQSNQLIQEQHISEAELETENQCEINHSDLSQISSIKEEQKLQIQLKFNGKNEQFCCTFDTFETISQILSSSFTDKQIIQIKIHILLNFYDENQDGSCRLWCIKKLLAFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1K3Q6</accession>
    <name>A0A8S1K3Q6_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0140326</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000011">
        <property type="protein sequence ID" value="CAD8050110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1K3Q6"/>
    <dbReference type="OMA" id="NCIGNGQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035777257" evidence="2">
        <location>
            <begin position="18"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8050110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="112" mass="13019" checksum="F0FBDF43E559E692" modified="2022-10-12" version="1">MIKQIIFILLVAFVIQARHQHLRHTKHQLGHNLSMKPGESCLPNTDLGGQKKEETSANQARKHLSKFADHLTEENRKAEDKQPYPNIYTYQDQNCVGNGQDPSKRDRRMRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KDD4</accession>
    <name>A0A8S1KDD4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0190292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000016">
        <property type="protein sequence ID" value="CAD8052657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KDD4"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8052657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="25371" checksum="94047DB1F35DA565" modified="2022-10-12" version="1">MMMNLRKQTRETKVYAKVSVFCLQQNNHNYHNNLLTPNQQSKIDLKQNQSKQIYSLSQNKIDQKNEIIHTNENKGQKYFKFQPVKYKQQLKLQNLQRFDIAIKQENSSIIVVQMMIEQNYNDELKDYQKKNQNFSLNFRNTLVNKQILIVAQSGIKNYQRGTHQFIMQLYIYFMILLIYYYQMEQIHRLKIEMNQIHETRIKNYFKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KGS5</accession>
    <name>A0A8S1KGS5_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P-type ATPase A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0220005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000020">
        <property type="protein sequence ID" value="CAD8054389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KGS5"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF5">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="2">
        <location>
            <begin position="64"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8054389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="407" mass="46783" checksum="D9BF3A9145B1FF34" modified="2022-10-12" version="1">MVLYQQFKFCYHAIIIYSQLKVGFLFSYVAPFAFVLILTLLIEAYDDFQRYKRDKQANSQVYTQIEKDKTIKLQSWQLKVGDIIEVHANQRIPADLILLNTNDITGTVFIRADQLNGEKDWKLCKAIRHTQNFEDNLKIFTAQYEKAKNQMGERGLANDLELLELIGVEDMLQKDIDATLESLKNAGIQIWMLTGDKVETAQCIAISTGLKSPTQEMFVIKDIEDSLILKKELNQFAPKNNSVLVLDEQSLKVSLEFQYTAFFHVACNSPAVVCWRFSPTQKAQVTELIKEQTQKIVLCFGDGGNDVAMIQAADVGVGLVSKQVALASEFSVLSLSIQMFYYYGMVDWLIKVYLKWLNSSCIEDQFIVSRQTIFSIVLYFVFIPIYNGWLMLEYGTVQHYLLFFLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KI75</accession>
    <name>A0A8S1KI75_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Xylanase inhibitor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0210206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KI75"/>
    <dbReference type="OMA" id="LINPHIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Xylanase inhibitor C-terminal" evidence="2">
        <location>
            <begin position="197"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8053933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="381" mass="44763" checksum="35A5E5FD9D51DBEE" modified="2022-10-12" version="1">MLSLLLVQIVSSMPIYGNFSQNYYYVELNNKRWILDQNFDGAIELLLGCENNQSKFHHFQAQRFILIGAIPLERKMIGQIDDVNYICNDTSCHMKIGDLIIQNFCGQMYTYFQEINNYLGLGGIIQKFNSQQQLKLCLAQNGGYMKIGNFQKDDQIIQIDQMKSICNQESNCQQNRLKLISLEIKQNFLFPNIYETDAEIDFTNTYVVFPKNLYLQLIKQFLCQGFFCPQRNDIDGLICYDNDYNNIDKFYEKFPKISFLFNESSEFIWFPQDYLVTHDNKTYCFPVKRGIGQIILGQPFMRNKEFIFGREKLIVNYQNCSLETSLDAPTISISIPLIKKLEIPKFLTGISLFIFTFIIYYILFKIKLRKTNQQLINPHIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KJW9</accession>
    <name>A0A8S1KJW9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0230363</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000021">
        <property type="protein sequence ID" value="CAD8055800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KJW9"/>
    <dbReference type="OMA" id="TQQIKFM"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8055800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="186" mass="22576" checksum="0C8ECCDB91BC957F" modified="2022-10-12" version="1">MQNVNKYRLNCKLLIESRRQKQITPQKVNNTIENILNQFNFIESNKTQELILQQNLNKTQQIKFMEQEQRSITNSNVTKMNYFKAKQDKFAKNMNQLKIEYPPLIINIKQLNVKQMRKPDQQYRRKRFITTQEKYLENDNTNNYIKLISQTRCVSNTRQYQNQRLQTFCSWSRKSSDNLSLKKHLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLU9</accession>
    <name>A0A8S1KLU9_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0210243</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8053992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLU9"/>
    <dbReference type="OMA" id="KQFEWIN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8053992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="202" mass="23385" checksum="903AC88F66BD5CA3" modified="2022-10-12" version="1">MQQNQCVQTSSFELAFQLQDLIYRETKIKSPSLQYNFSTSKKTISNSKQIKQINSHRSVGEINSNISSPPPKKLLGKPVEGRALLANLMFPSQAKMKVSIIEQNKEKKDQQTQKNIHMARRLSQYNQNESKSSKQFEWINKFNLQLQKCNYYVNNEKSVKLKTQRKRQLSVLDDVLPISSRNLFVNGTQSQFSSRVKTPNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KLV7</accession>
    <name>A0A8S1KLV7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Purple acid phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0210255</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KLV7"/>
    <dbReference type="OMA" id="INRTHIQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00839">
        <property type="entry name" value="MPP_PAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041792">
        <property type="entry name" value="MPP_PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025733">
        <property type="entry name" value="PAPs_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45867">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45867:SF10">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14008">
        <property type="entry name" value="Metallophos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035768176" description="Purple acid phosphatase" evidence="2">
        <location>
            <begin position="18"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="3">
        <location>
            <begin position="149"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase C-terminal" evidence="4">
        <location>
            <begin position="383"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8054016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="504" mass="59184" checksum="1194F691DF1484A8" modified="2022-10-12" version="1">MVFIILMLLLGWTQSQGCQPYGIRLFLGHYFSYLRNSQDVIRIVFNTEQECRGQFVLELMDGKTDKQLMTSQITYYDTQLLNMTQIYEKNQTTHYNYETFIHTFKIGLNNLDVNVSTSYNYTIVSRQNSILSGPYKFDIPLAQNEQKMLVFGDMDSNWVYNYSKDTFNWLENQVKSDKRYDTIIFTGDMAYDLESHNCKQGDNWLRNISVFTNRYPFMATPGNHDNGNNTFYDFFRANFGALFLKEYNTQHYLNDFFSFDVGMVHFISFNPIKAVYHNDVNNITHLIVEQMRNDLIQANYNREKVPWIIVYTHYPIYCSAPNSDQCLNNFNYLSAFEDLFVQYNVDLYLSGHVHTYQRNKPYYKNNIAQYSQKDNIISKYQYPVSIIEGIAGTDYGEQNETFPEKPFIEIQNPNHGVGIITIKNSTHLYYEHITVADNKVIDSIWINRSIDINNKSKHDGFEIVMWVIFSILMVITGVSIYFKSNQGKKNKNKLLDDTIKMVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KM23</accession>
    <name>A0A8S1KM23_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0240237</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KM23"/>
    <dbReference type="OMA" id="NFIEYKP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8056288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="144" mass="17156" checksum="79FEDD25DF7C0767" modified="2022-10-12" version="1">MINKENFLNKAIQRIRLDEITVKETYFQDDLNQEDRLKEFEQKYHGFRDNFIEYKPNKSKADNIDNQQKLIQALFPGIMMEDITAGILANVTLLYIQEIIETAKELAVKNESLKISQNDIIEAVRIAEFKNQYELEEEFQRFFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KML7</accession>
    <name>A0A8S1KML7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0210370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000019">
        <property type="protein sequence ID" value="CAD8054246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KML7"/>
    <dbReference type="OMA" id="AHLCASR"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037696">
        <property type="entry name" value="CCDC77"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22091">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22091:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="418"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="175"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="322"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="418"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8054246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="500" mass="59566" checksum="7054FFCD818F213A" modified="2022-10-12" version="1">MSQRDLSNLITINPNDPQQYQDLLAYYRSKLGEFEKERFEWLTKLEEIKIQYEDKHQQEWELLKRKQEIKELQQTVGEAKLMLFEERQARLKLQKENDALKVRELEDKKKIAELMAMIEPIEEQVVLQKDLRPEVTTKYTGDTLAVREKQGNVKMHNINQGRSILKTVYMPNEQLNAYQLENENLKKQVENAETMLSQQFAALREELRAKEVETNLRIKEDQQKLDQLVQKIQKLEKQNVELVKDCYVLKQKFQNKEKQMQEDLEVSKLKNKKLKMELDQIQRKYNVENKTALELLEKKSDEYNKKFRSQIKSKDEQLSIIKEQYEQVQNIYINKIQQLEENLSKLIEKYQQLEKRRSLEIEGFKNDIKILTKKVKDCEKSQVEKQNENKSPEELAKYQEEAQQIKQQLAKETGIIYSSGKKNNSNNQQQTIKLKGNQQRGASKTMKQSNKQTNKGFNNQNQIDQKEDDDVSESVPMHELEDLQRQLDELQYQMDKAKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KPY0</accession>
    <name>A0A8S1KPY0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0240333</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KPY0"/>
    <dbReference type="OMA" id="KCNDMMS"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="104"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8056481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="308" mass="36751" checksum="EE48FB8B25D745CC" modified="2022-10-12" version="1">MSNQDMKEINDLLVLDYDVFGKCNDMMSFIKSTQPIQKDDPQICKKVNAVAKLINQLIKFLKDSYSSNLDNLPDYYDIVYLLNNNLQNNLTVQAKKMQLIEASNYELKNQITNQTQKIQQLNKDLQIKDKENQQLRKEIKALKHQIQQLEQLGFQVEQEMNDQNYSLNEDNNLLQNEITQLKYQIELKENTIKYQEGEIKLLKLQLQRIQGDQRQDIKHHLFYDQQQIFKENQELLNKINQLSFKHNYQFIENVEKGKEHQNVKENKIQSQQIEIMQNRIFNFLNQLSLHGFLISAWIGAYVIIQYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KSR8</accession>
    <name>A0A8S1KSR8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0270226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000026">
        <property type="protein sequence ID" value="CAD8058449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KSR8"/>
    <dbReference type="OMA" id="SQRFDKP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8058449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="74" mass="8481" checksum="A11EEA558C98D5CE" modified="2022-10-12" version="1">MDSIQSFFSSNSSFTPSSPQKTRPLPSPSLVSLEEINLNDIFPEELNSRKSSSQRFDKPILVIEYLVEKLKLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KUE8</accession>
    <name>A0A8S1KUE8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0240256</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDM010000022">
        <property type="protein sequence ID" value="CAD8056326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KUE8"/>
    <dbReference type="OMA" id="INETALC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="2">
        <location>
            <begin position="623"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8056326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="959" mass="113292" checksum="75A0E44C8518596F" modified="2022-10-12" version="1">MSKQNLSNYIKFLNHLTELEQKGLLTSKQKHLLKVNLTTKEQILDKLICSFDDEQNYQDAILQYLSAIEPQTKFRCHSSGESETPMKFRKRHNSHSKITSFNESKLIANLDELNDQLMNIWQSDTQFLFNQEINEKLRKVGELINKTATSTPFGSQKQVISNSETICLIDSLRSLYKDMKQHFNDYLRCHILLMDDQFTTEHLYKVLRAFIKNLVDADEVTFIINNELYTSRTEQFQNIIITEQQQQEINLIQDKKLIDLSQFYPELGVLFQTNQYKGNYALKISNSSCIFCFHQKGEKNIIIDSFLSLCNEYQFFDEVNQLAYFLLETIKQARVQCFNPLQLSHMIQDIGIAFIRCSKYIFIQKVIEILQERYQVSKENTSFSSGQPFIEFDFRDSLNLCIYICMDLNKTEEMNVYKQLNLSYNKYLKFIKQCYDRTTFYKFFVKSQDSLIFEFDKQGLITFVSQPITNKLANDFGIKFNPQMFNASYKSMFKNKNLLQHIENQLQEQNQWKLTDSNSTYEVFMKMEEKYFKGFFVIFQQGWFRYDQKEFDASQWNKLRKSIMLSETKQYLDKLEQKHPQLKNSVVQMFKPKSTQKQSLFKNFSPNFSPNHLDQRISLKESENDRRIPKTKGVNHFYSDMITDTCIENINNLNFNIIPYSSDWLEKQKVVFQILRINNFIEDYKINETALCNFLCALEYKYNKSGNKFHNYDHGVTVMQCSHAISLEMMKTKHANLLTQFIKFALILAALCHDVSHTGRTNTFEINSLSNLAIRYHDKSVLEQHHAATSIKLLCAQSTNIIPNFNSEEFRDFRKLFISNILFTDITEHFNLMKSFEARIQELNFGTEQDVRLMTGMIIHTSDFAGGAKPFPISKEWSIRVNKEFQDQYNLEGKFGYPQLPYMKDLDQLPIMAKSEVGFFKFIVRPLWAVMSKFIEDRLQESIDNLDYTIIEWEKLMIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1KZN3</accession>
    <name>A0A8S1KZN3_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0280324</name>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0360308</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000027">
        <property type="protein sequence ID" value="CAD8059285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1KZN3"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8059285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8064802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="146" mass="16413" checksum="8AF912457128D75C" modified="2022-10-12" version="1">MSNTFKSAGNTLAKGNDLGQTNQSFGGSQNPASLKGKLMSLEETIKGIQDEMNFHKKEVQILKSEKDTLESVLSMKTQDVKKTLTNELMRIEEEMKRHFAHQKAENSRLQQQITGLKGEKTALQQQLLGLQRRIAELELQVGQEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1B0</accession>
    <name>A0A8S1L1B0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0320030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L1B0"/>
    <dbReference type="OMA" id="YNQVEQV"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="76"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="203"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8061588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="455" mass="53741" checksum="07488CD59683A60B" modified="2022-10-12" version="1">MQQYQVGRRNPSQPQQQSLKYNQYQSRSPFQSIENTQNTQMPPNKFHHKASTDIDTINKNTSIATNPYYNQYLKRASNSSHQDKPPSLQYSNSKSNINPNQSHDYSIQFKVGGKNEKKNDSINETQLSQFINPTKQFVCMSPAQNRDNSIIFQSPKQTQQTTIVQSPQPYNLINTPIYYNNNKGSQSQLKPINYSNPNLFNEFKQLQDSNNLLQQTIQQIESEIQRGQSSQFIQELEQKINLLKQLNDKLQFENQSLIEQTDIIRMKENLEKSQQIRKQTSQQLQNIKLEQQQYQQKCDELQNKLDQNDDSQIKDLVIELENKVQNLITENDRLNQQLSTNSNLQMISDIARLKNEIENLNKNQQQLKMDEEHFKLTYNQVEQVYQQKMEENIYADLNEKIQLLIEENERLQQVINDSEYTDQDLQDIKARIKVIKQDNLKMEQHLKRKNLNKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L1G4</accession>
    <name>A0A8S1L1G4_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0320143</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000031">
        <property type="protein sequence ID" value="CAD8061780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="DCKYFAN"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1099"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1155"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1239"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="234"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="309"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="495"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="553"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="874"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="982"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1107"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1155"/>
            <end position="1172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8061780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1294" mass="155432" checksum="DA583BD46D2B9E38" modified="2022-10-12" version="1">MQNSIDEFEFRMNEFSELLKQDLFTKDGNQKLQNDCKYFANLFSDLLRENYKLKDQIIEHLRQISVYREELQKITFDVNQTTNEDRKFSKDMASIELLVKYLVEDQRYFYVEKEKMINAIKQKDKEFEMLKEQHILQIKDLEQQMLNRKMKWKNRFKKQVQDGLDNVQQIKLLEDKILQLNSEIEQHQQNILSYESEITRKDLKLQYYQKLEQEFQTKQTDIDIKHDFAMKELQKKYRNEMKEQKIQYANNQKQLQNNYEDKFQILQKNAEVYQVQLEQKTNEFLKLSSEYDALIQLYKENKEKYGVVVQDLENSIQTQKQEKQELLEKYTKLNEDYELTKKQLKSSKEEVFKQANDIQNQQKEIKSLQLNVQALENKMKEKVERFQRLQEQMKVDHNKQCQKIKEDMEKQSDNRLNQLQKNAQEQYELLTRQKNTEINRLKGQLDTSISKFEQYELIHKDQISKLEQQAQMKQIDFNEKVVKLKEQQNLQEIIIKNKDTEIQQLNTKIQLQVDEFKMQLKEIESKFQEEIIILRDGVEILIKSAKKPLEDKILSLTASCEQKQNQLGNLQNQLNQLQSKCIDSEQKSHERIIQLQDEMNMSLNIFNKMNDNLTTKLQKMMEQTKQIQQNYFILSEQVQTKNNELIQLQQEINNKEYARQQENHKHQKLVKELQIFIGNKELELQQLNNQYIETLGITQQQNIKIQNMNYQYQQEKESLIQEIAKKQNLIIQQQEEITLAKQSALQQKQNLEQQLSNQKSNYEEQVKILEENQIKLELIDKEQQTEFQSNNIIQQENQEFQQFNEGKDSELMKEMELNYQKEKAELIIRYEQIIEQLTQATQQEGNENQEQRKPIIQIVEKIVYKEIPKKENHIQGLDVIVKQCQKELEEAKEKAQADKQESQEYVNQLTLKFQDILKENEKELDDMNEQVQILKNQITQLKKDNNMLKSDMIEQELKYQLQIQQMKEQSDVQLNTLTETCNQEIEEYRKKYEEKYTQFEQLRKQYQERLARPDDTETIQRLLKDHQELYHLVVNAYEKVKEAGDIIKYLDLEVQNYKKANDIFGGSQISSQPPPSLSTQKMLQRVAQIKQDLEVTQNKIQSKKSDNTLARENTNNQEERQLSHLSDYSELNSKLQLDSKLPPKRKKLPKMMTSMRSNLSDAQPSSQNQTQRQIEEKPEVPLFKFDQDDQVIIEAPVSFNAFVDQRQHGSQMSQQRQNKYTVYIEKKKKDMINISDIVQQQQVSPAESRYTRRRQEQQQQQQQQPKKFVPYYLRNPEVQPRFKSQKKDSSFKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L3B4</accession>
    <name>A0A8S1L3B4_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sulphur transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPRIM_AZ9-3.1.T0290301</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8059992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L3B4"/>
    <dbReference type="OMA" id="CSIHYIT"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046513">
        <property type="entry name" value="DUF6691"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp_TsuA/YedE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574:SF1">
        <property type="entry name" value="SULPHUR TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20398">
        <property type="entry name" value="DUF6691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8059992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="39062" checksum="F269E0A365074C61" modified="2022-10-12" version="1">MIINISDLFLSLIGGIMIGVACSIHYITKGRITGISGIYYGVITFNWDEFYWKLSILTSLVFSTGLMYQIFGDESIMTSSSLAFNEISQITSLSYTIAGISGLLIGCGAKLCNGCTTGHGFCGLARISIRSFVAFGLFILFGVLSCFITMSEHKDDGEEKYNYNYVSIIMIGLSLVLLSASLLGAYLNKEQLIDTIVGIPVGLFLGVGVVFSGMIKRTTILSFLSLQQWNPQFLFVILGANIACFFGFRLMKKPIFDVEFQYPPTNKVTNVLLVGASLFGFGWGMTGICPLVGLALFPQFTWQIGLMYLGSVSMGMRIAQCYLNKYQKVEDQVLQYNSNNYIIKNDNEQNDRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L541</accession>
    <name>A0A8S1L541_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0300263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000029">
        <property type="protein sequence ID" value="CAD8060693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L541"/>
    <dbReference type="OMA" id="VEIMICK"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="107"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8060693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="397" mass="46970" checksum="DDBAEB705EEE99BD" modified="2022-10-12" version="1">MNNIVYYKGSLLRKQNNDYERRYFYLFENFLIINKKEDHNLIDRKFDLTSMDCQIKWHYEDNIIKEMMVYNTKLNQRIVYCEKHEILLDLQNKLDGKAYYRNFKSFYQKLEIIRSHKNVEIMICKNILTDEIVIAKQVKFHKIKLADLDLNSFVMKEAKIYMKLSYNPHQYLLGARQIFTCDQSVIYIMHNCKGGTLYEYLIENDLNLSELVTQEIMKKLLMGLNHLHKLGIIHRDLKLDNIMLLRKNDPNSIRIMDFGYSTFIDDKSLSNQRCGTPGYIAPEILNMNHYNELCDIYSLGCVFHALLTGRKLYQTPKSTKASELLIMNRNSTISLSQISNLNALELLQSMISVAKERPSAAVCLQHLYFEEEQFQIEDLSTQLRQLDFPQLRNPFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1L5H9</accession>
    <name>A0A8S1L5H9_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0290308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000028">
        <property type="protein sequence ID" value="CAD8060006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1L5H9"/>
    <dbReference type="OMA" id="CENDTAY"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="279"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="328"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="492"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8060006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="68474" checksum="DC0742E8028621CC" modified="2022-10-12" version="1">MCFQVNFDSKHRIYKRLSIFYQSFIFAYTLAILVLSIFAWNQFSELTKPYVDRIQNWELNPIYEVYKQDTCESDSEDFFKLQQGQTYYTKWTNSNQVFSLCIRRLKDYNFVKKYQIYPKCLDTEELCGGVYVQNKYCAPQGQCPINSMLFVNGEELKKVDKTKYTNQLQINDNFYLVTSTLAEALPINQLEIFGTTPCIISQYTNYDTIFSNAFCENDTAYTLLNQIDEMTFATSNGFTSNTADKVGIYSRSYTQFNIECRQQNLITWGIRTLKDSNAIYPVLSILMIISIVHFGLVGFVQSLYYFMQVQYQAPTNKNVHQGLLIVKIFIQYFHSTMIILTFGLWMDIKLFLQISYDMNCSDTQTDTFINNELDNIDKQWRLSFIMFLLFVIFALLECTAFSICLDKKLKKKIEPKYRLITFSTVNQSQLGTKSDNSNLYMYFKPQFNLQQELNQLDAKQIDQSEFPNQILENQQQLNNNNEPSISMKQSNSIDPSLQQSIQSPIKQQSLDQQNQNQTPYMSQLKQKQSQKFQQQSNIIPPQQQIDLANFQSQQVPIQKPSMKYDIDDQIDSEEQNNRTYQVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LA85</accession>
    <name>A0A8S1LA85_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0360106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LA85"/>
    <dbReference type="OMA" id="CNNVIAP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035719874" evidence="1">
        <location>
            <begin position="19"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="76" mass="8053" checksum="114101C4641C12A1" modified="2022-10-12" version="1">MKAFLLVCLIATSMMVNAIEIPKDQVLSGQSDSPSFLHVQTFQACQFCCGGKCCYSASNCVKGVCNNVIAPTCVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJ32</accession>
    <name>A0A8S1LJ32_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0370234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000036">
        <property type="protein sequence ID" value="CAD8065363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJ32"/>
    <dbReference type="OMA" id="DIKEIMC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="173"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8065363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="572" mass="68254" checksum="85F343B76A619231" modified="2022-10-12" version="1">MQRPLTSITSSQQQSKLYDSSSTSFRIKENKKNRQMPQQMLYDKEGLYEENLKLKKELQQVKFELKKERQEHANFEKQVQKTNETQKDFQVILSQRARIKEQDKLIKELQDEVFKLKRNPEKTKNKELEAELEEQKEAYQKLQLYISSKCNQDANETVMINQSLKDQIQINLVQAYKQDNLNLSQMILSLQQENHTLQQNLLSLKNEKDRLNGRLKELEKQFDSVNKHMMYMRQEMQNTKKQTPSEDYLKKIDDLQQQIQNQGKQLQQNKKLIEELEQKNGELQHNYDVFKSMESKEKEKLQDRINMLKKQIEELNQKRDDLEQSQEGSLQSIGLKQAMGIYGSPRSNNSPSKLQLQLPVTNKKQCLKVNSYDIKEIMSNLKIKLLSNNIDYETQEQILCEDDDEFLTLEETQLRLQKSPFELNEDESSLLARYLIEDNTEDHILYDRQRTQTKIIIKSVYKKLLGEFNLFNESEKSQMYEYFCQLFHKYNQGMESAIKQFNIKKKYLGNNQCEYEDFNEALKYCDILLTSRQRLYLELEIFSDSQQLQRFNFQTLFSKFKLKVSDLDNSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJ74</accession>
    <name>A0A8S1LJ74_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0360290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000035">
        <property type="protein sequence ID" value="CAD8064766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJ74"/>
    <dbReference type="OMA" id="DQNKLQC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8064766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="409" mass="48466" checksum="D9D91D84DEA071EA" modified="2022-10-12" version="1">MIEGDEEDEINNHNEFYPIKSKIRNKQKVNKNTHGQFIHIFILLIFLAISILIFYNWIDNQSQEIQQTQLLPYQNPQDQNKLQCPFNTSFDSTKFTCLQCTQNCISCYHEYESRCLQCQSPFKLKQNICTKDCVSINNICVNTNNTQIDQINYTVIDLGTLLRVEQLFQVEIHTYSYKNIYIIFPPLGNSFNYFYQSIQLPELAKQLESTIFVFSTPYTEVIQIIENKDQQDDQFIYINRIYQFVQPYFLNQTKSINIVALGEMLFFAHKFATLIAHNLNQDFKIRLILMQTNEVELQESIQLEDQKLQNILKFINYKWNNNTHQELDINFNEQNISQQLQSIKLKINIKKIINSVHIRRINVIIISERHSKDISILYQNGHNHYNLTQIFLDNNKWSALFSKLKGLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJG6</accession>
    <name>A0A8S1LJG6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fumarylacetoacetase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0400030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000039">
        <property type="protein sequence ID" value="CAD8066939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJG6"/>
    <dbReference type="OMA" id="RIMTLYP"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="1">
        <location>
            <begin position="15"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8066939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="224" mass="25478" checksum="B25ABE85B69776E9" modified="2022-10-12" version="1">MNLLFKQVVNPNNPKIVAIAKNYVKHVKEMGGDEPPKEPVIFQKPFTSLLYLPDGNGILQLPKHNHEIHHEIELGFMVAKNGKNIQKDKWQEYIGGYFLALDLTDREMQAHFKKQGFPWDLAKGQDNFFPLTDLITKEKVKDPNNLQLELKINQKVVQNDSTSSMYYKIPDLLAYISQFMTLRPGDLVLTGTPHGVGPIRAKDNLFGTLKQGNETLAQLNLIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LJQ0</accession>
    <name>A0A8S1LJQ0_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0410283</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000040">
        <property type="protein sequence ID" value="CAD8068020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LJQ0"/>
    <dbReference type="OMA" id="WEFLANG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="355"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="401"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8068020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="441" mass="51745" checksum="97548813EF2A966F" modified="2022-10-12" version="1">MNYQTVWDQIKFYEPHYKDFNYLDLQKVNARLAIANNYIYYGQFENNQRHGKGILLQSNGRKYEGEWQNDQKQGIGWEFLANGSQYEGNYIMGKPHGQGKFTWANGEVYEGQWNLGIKEGQGIWHGLKGELYQGEWSNNVAFGYGEHILSNGDRYVGNFKDWLKNGEGQEYFSNGDMYQGNYLNGKPHGYGEYYWSNGAVFQGYFKDGLRCGKGIWKRRVDSPTDLYQGDYDEDKKNGYGVYKWSNGNEYHGTFINDYKNGYGEMHYVDGKVLKGNWEQGKLVNEIKQNSPKKPQHSFDNHIKIVDNSNNFNKNCQNKNKNIDIQYDNEIQNNVQNEFDNEMQSNQYIDASISNKNIHKNNNNPVSSQTHRTTKTIRIRQYQKDKSFSMSQQNSQYGPSSPKRDISERNEKQKTQTKFCIKTSDNFYLHIRRPKQHSQYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LK74</accession>
    <name>A0A8S1LK74_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0390200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000038">
        <property type="protein sequence ID" value="CAD8066612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001094">
        <property type="entry name" value="Intraflagellar transport particle protein IFT88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001801">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002293">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44117">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 88 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44117:SF1">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 88 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="195"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="507"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="541"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="575"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="655"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="967"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="1035"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="1406"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="1440"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="1474"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1595"/>
            <end position="1642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1628"/>
            <end position="1642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8066612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="1642" mass="190805" checksum="B194DBEFBC9D4473" modified="2022-10-12" version="1">MNSSQYGAFGMSGNNFNQAKPPVNNYNFENVRAQQPAPGGPQPSRGKPIGTAARQPQAEARPMTSNRGANFGQKKDPFNSTQNQLNLNKPKLETNPEEQFKSIEKEINTLIEQSAMAKLRGNLSECLEKAKEAFNKEKKLRQSKEAQNLAESINTDLSYCVALTQACALHANGLHQDALSKYQEIIKCKQYPQAGRLRVNMGNIYFEQKKYPNAIKMYKMALDLIPATSKEMRFKIQKNIGHAQVRLGKEKIKEAITTYEQILKNSPDFPTGFNLMICLYLSGNKNKMKDYFVTLLTIEIPGESEEENNENKGTTITDKLREDTKERRREAIYYIVTSAKLIAPLIEDDIIVGYEWILEQLKNSTFPEAETEIEICKAMAFLKKKNIEKSIETLKGFEKKDKQIMARIATNISFLYFLENDYKQAEKYAEIAITYDRYNAKALVNRGNCLYVKNEFLRAKEQYLEAIGVEADCIEALYNLAYVNRKLNMFVESLQALDKLQTIVCIPEVLFQMATLYEMTGNSKQAMKWYLEVLNKVPNDPNILARLGSLFAREDDEPQALHYFQESYRILPTNIETISWLGVYYVKQEMYEKASLYFERAAQVQSRDVKWKLMVASCYRRMGHFQKALGNYQKIYSDYPDNIECLRFXQYPQAGRLRVNMGNIYFEQKKYPNAIKMYKMALDLIPATSKEMRFKIQKNIGHAQVRLGKEKIKEAITTYEQILKNSPDFPTGFNLMICLYLSGNKNKMKDYFVTLLTIEIPGESEEENNENKGTTITDKLREDTKERRREAIYYIVTSAKLIAPLIEDDIIVGYEWILEQLKNSTFPEAETEIEICKAMAFLKKKNIEKSIETLKGFEKKDKQIMARIATNISFLYFLENDYKQAEKYAEIAITYDRYNAKALVNRGNCLYVKNEFLRAKEQYLEAIGVEADCIEALYNLAYVNRKLNMFVESLQALDKLQTIVCIPEVLYQMATLYEMTGNSKQAMKWYLEVMNKVPNDPNILARLGSLFAREDDEPQALHYFQESYRILPTNIETISWLGVYYVKQEMYEKASLYFERAAQVQSRDVKWKLMVASCYRRMGHFQKALGNYQKIYSDYPDNIECLRFXYPNAIKMYKMALDLIPATSKEMRFKIQKNIGHAQVRLGKEKIKEAITTYEQILKNSPDFPTGFNLMICLYLSGNKNKMKDYFVTLLTIEIPGESEEENNENKGTTITDKLREDTKERRREAIYYIVTSAKLIAPLIEDDIIVGYEWILEQLKNSTFPEAETEIEICKAMAFLKKKNIEKSIETLKGFEKKDKQIMARIATNISFLYFLENDYKQAEKYAEIAITYDRYNAKALVNRGNCLYVKNEFLRAKEQYLEAIGVEADCIEALYNLAYVNRKLNMFVESLQALDKLQTIVCIPEVLYQMATLYEMTGNSKQAMKWYLEVMNKVPNDPNILARLGSLFAREDDEPQALHYFQESYRILPTNIETISWLGVYYVKQEMYEKASLYFERAAQVQSRDVKWKLMVASCYRRMGHFQKALGNYQKIYSDYPDNIECLRFLVQLCREMGLPYEEYAGQLRKLEREMEMMDGYQGQDINLINNEEEQVRLPQGDDNPVSFTNNTRRGNKQPPPKANVRQNIDDEQFQDGVEDNFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LKU7</accession>
    <name>A0A8S1LKU7_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0430010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000043">
        <property type="protein sequence ID" value="CAD8068808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LKU7"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8068808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15989" checksum="019ECF5439073531" modified="2022-10-12" version="1">MNQATPKSSGINYASNFFQYQELRKQNRRNLLFPKHLIRICSLIINKIINIDLKNISAFHRIILRKTINPNRKFHFSNNISINPNIYCHIQNLQLNKYNIILNLFKYSFFYFFIILIFLSIAKEDGKRTDKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LNN0</accession>
    <name>A0A8S1LNN0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0420149</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000041">
        <property type="protein sequence ID" value="CAD8068419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OMA" id="PYANESH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035825338" description="Transmembrane protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="2257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2190"/>
            <end position="2212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2234"/>
            <end position="2257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2247"/>
            <end position="2257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8068419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2257" mass="261802" checksum="50907A47F38260CA" modified="2022-10-12" version="1">MMVRICVMLLVVIQVLSKKQKDFLFDLKQGKSIQVELPEKQDKDYIILYVRPQQDEKKSDLGEIQLKSKDEEFKLNEFYETALFTIKSDEINLKCISIPCKGRITIINNDAIELNSKSTELILSQKIPVQVISMHLSGSYNRMVGEFKLEDDESDKIKIEVFEEKAKLYGDEVQLTVAINKKDCDKCKLLAVLSGDLNIKEEATVNGQLYLYKDEEDIEINQEIQDYLFERDENIYKLILPQKFNILQIGILGDMLPLLKVHKKDNIYFNSQEPIQLQQRGQAFHIILRREDIEGDEVTINLSQGGSMKYLMVVKLIDEAILYNNQLILSKIQDKGKHNYLFKTIESEQEVGIKIVYFEKGDNSIRIAECQNGCQLNDEDNHNLRFHKNEQKPAVYISPHCQNKDKEGFCIYQLELSSNNKNIYMMTIQNDNNQRILKSDVAYTNYMEQSQEEHLILKYQKSDDDEELVFTVNTHNIVYMISRDSSCYPVRIECAKYFGGVDHLVVLRGDQLKHTQYYITLTAREATIFQFMAKVTKQADINIQVLKEDETYRGVLQLIDGQQKIHYFRVIINFNQVDQNVEVIDEQQPTIEFAIHSSRMQVALTLNQGNQLPSLKSYDLITFNNYLSIQPQDQFYQNSGNYTIGIQNMFETFLNKEIMYTFTYSTSRTVKTLHVGQQFVDLVKGFKSKYFSFYYSQNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNIQYLNIKNQMMMKNWYLQLILIILDSSCYPVRIECAKYFGGVDHLVVLRGDQLKHTQYYITLTAREATIFQFMAKVTKQADINIQVLKEDETYRGVLQLIDGQQKIHYFRVIINFNQVDQNVEVIDEQQPTIEFAIHSSRMQVALTLNQGNQLPSLKSYDLITFNNYLSIQPQDQFYQNSGNYTIGIQNMFETFLNKEIMYTFTYSTSRTVKTLHVGQQFVDLVKGFKSKYFSFYYSQNTTLFYISIQSKNNNKLTLTVQNDIKPDNHSQFAKSQDSSTLKLSQSALNSLCLNGLTQEDKEDDIDVMAICQAYIIIENHGNEDILFNINIWNPQTALELKDGQEYTFNLEYLLQQTFLYYKVISTQTDVQLHINSHYGYTKYQIQILNSQNQESNSLYAKEEYKTQHSKSIHSDAFEFCKPDCVLKILLEAQELIYQSDRIINFDDTVYVTVTQQYMDLKSGLPIQISVNKLQPRKFIFSEINDISPQSRFKLILHEIYGKGSICLFFNDEDNININKCEYEVLGNVLELTQAQIQEKLTELNLTRNPYIIIQIYSIVDNSKFQLSLEISNDRNNHKLMMGIPTRIKLDIQEEVQYQYFNIQKDDDLYFKFIKVQGTSLIQISRCLDNIQKDCEEETIINEQLLAGQTYNQHIIHKNDNKKYCELCTYIIKIKTIGINVDLLIIVTSQLNFVQLPQNIAFTDYLENQNDYNIYHFSYNTDHQIEIQINQYNGDTQMWIGYNAVLDTSLYQYGPYNNIKQQKLMNTTSSISYYQAIIPPREHLEQTNYTPYANGSHTLAGHYNDDDLYIIVKNNQQKPSNYSILVTQSTTGNGQLLQDGIITFAYLSRQTPVITLYHQYRYLKQPQLVIKMAHYSKQLQVSDFFRIEVSNETEPVNYTLLTPFSSRNDQQTYILPILEGLLTIKIHSLLEIKGNENINIEYQFNREGDIVPIRKQKIKDNYNYGNNYLNRIDLQISIVGKEILMIDEKSSLSDQIYDSQAKYYESYIPYEGKLAVQVYNCLGELKVSITQNYDEFIEGIYNGSKITVEGQLTDIIVPVKPGPIYFQFQSIESVYKFTTQVYKAQDFAPFGQLVMGGDGQINYSFDTYDSDYISVKFKPLKCVGCDLHQEMNSLVKYSISWGSTIQYAHVIGLCQYNQYANYHKNHTDNFEQVDIGLFALNHTDQITFNIIVGNQSIHSQLFIAIRAQVLVFNNLTIQDYELYYHVVQIAMPNLSLYWYKHRFNEFVIGVVIVVTIILSLSCVLCKIYRKIRQLKKENLNLQLEKKMEEAKEKGQNQTKYETLQDESNDQSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LV35</accession>
    <name>A0A8S1LV35_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0460291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8071089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LV35"/>
    <dbReference type="OMA" id="GANKEFH"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050593">
        <property type="entry name" value="LovG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070:SF6">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8071089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="186" mass="21296" checksum="2FD865AABB386D32" modified="2022-10-12" version="1">MQNKKKILCLHGNGANKEFHSYQLRQFEKEFNDIEFVTINGPISITRNVHASQVVIPQNFAKMIENKPLFTWGNFFKLDNNNIDAVFQESLDYVIKILKEQGPFYGVLGFSQGSAVAARLATLIDDKQIDLGYEINCFIFSSGSMMTLPNKRLIFCQIPSIHFIGINDFLYDRIINSIFKSFSYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LYE0</accession>
    <name>A0A8S1LYE0_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPRIM_AZ9-3.1.T0460248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8071005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LYE0"/>
    <dbReference type="OMA" id="CWINTVG"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053083">
        <property type="entry name" value="TF_kinase-domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305">
        <property type="entry name" value="SI:DKEY-192D15.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305:SF24">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE C03B1.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="37"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8071005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="395" mass="46284" checksum="A3BF75AF951FD1A3" modified="2022-10-12" version="1">MYPEYCVAQRQQPYPLHKITQSYNPVVQNEPRPRQPNENPKDYMITAYTSINHVIDISPTSSIYRLDPFAKYLCCWINTVGPEEVSAIQERTKWKHPNLVSFLFFEEQSDKFSQNRQLKIFYEYLPLDIKQLIDKRSKTQEYVPEEEIWKMISGLCEAFIFLQKRGITHSALTLESVYFDEEYLLYRIQDVSPFRARAPHLFENQYKSPESGGNLRVNRFKQDVFGLGMIILSLCLLKNCDYIFETGVLSVVHLNDCFDQLKQLYPGRVDSILKKMLLIDFNQRPDWLEMQQLLVNLKDIQLANIDLGLTNKSSILNSSFQLQNQQQQQIQKTFDQLTLSQENRETINNNKLTSSINNNNATIKTTNTDSVDQKLINLNKRIQDTLQKSLNKNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1LZS2</accession>
    <name>A0A8S1LZS2_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0460297</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000046">
        <property type="protein sequence ID" value="CAD8071101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1LZS2"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8071101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="51" mass="5995" checksum="A6EE7616853B89BF" modified="2022-10-12" version="1">MNTNSYSSPLICIHLFLRGEFPPFPGSPIKQQNFSTFCQWKRSCLWKRSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M3U7</accession>
    <name>A0A8S1M3U7_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0490166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M3U7"/>
    <dbReference type="OMA" id="QCLWREL"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="217" mass="25882" checksum="0E154A70C117D809" modified="2022-10-12" version="1">MKGKVALLIDQDCEDFIKKGNTTIVIQEQEDFLLELMGMKEDGVHQIQQNQQNDEEILEVNLINDAQFYSQISYGAKTVVIGKQYQQFQLPSYDQYQEKLFDSFDIVIAFKDQIQVLEEIQKQKQHHIYFIISNIKQYNNDIEFPLQSRNQFKYQQLECQGKQIALIHYSNYQMRVDDIETLEQINQFSYIRRNMIRLQCLWRELLTQVGKFPKYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M420</accession>
    <name>A0A8S1M420_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPRIM_AZ9-3.1.T0490206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000049">
        <property type="protein sequence ID" value="CAD8072593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M420"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8072593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="227" mass="26532" checksum="14E77BC5D1F69C1E" modified="2022-10-12" version="1">MSSSIKTFRYCKIAGHQSKRIIRICVSNQTCTNRYLCELCLKSQQHQHIDQCISIDQFLQRINQIKMNYKDANKIEEISQILKNKINSCMSIIQQQFSLLEDRVEGIKTNIRNSFTNITQVLDQLASNPLGFNDENLNRNLYYLQEESWNEFQISVNKGSHIMKQYFNNLHEQSLKFKLTTGHSQQGSTAQLLQIPETIWRLKGIDQSFESQLPYHSQSITLHKLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M6F8</accession>
    <name>A0A8S1M6F8_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0510079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000051">
        <property type="protein sequence ID" value="CAD8073493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M6F8"/>
    <dbReference type="OMA" id="IEKQNIC"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="511"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="91"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="446"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="511"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="528"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8073493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="539" mass="64249" checksum="AE7BCFCE960FD424" modified="2022-10-12" version="1">MSKQQDNLFQNNKSQRTLRTEQSKQYETGHLQPTLKQVNSPHHSQSISPIRSPNELSIDNKQGFNGQPNIKNADQQIGALIQEIEKQNICIKLKTQQIQECQSKISQLELHYGLKIEELETQHLYQIQLTQSQLREKQQENANLINEINQLKSQKNKYNLLVEQTNIENNQLREQLRKFQMINSTQTGDIVDDPSSKQQKLEIIQIKQQMIRLNEVIKSLEEQNKYYEEECNRHFKENQYLINIQNDLTQQLEAYKKELLQFQEENKVLLEKINKSKEEFNISQEQLIQQLKQEQIEKQQLQNQYQQKILELQNVTSQLESKIQETQQTSSQMNQYSWKINQLEQQEKDLQNLVEHQTTQISQQRKTINLLEQNLQNSQNTEIQYQQLLQNYNSIKEQFQQYSISEHNAQKQYLEQQNKIDKLTVMLKNRLNEIELWKNKCYENSSQQNRQLLDQLIQENEILKQKCNILEINIKENSNPSQSQEINKLNLIIKQQQDKIIYQQQLLENQKKQMSSPKSQKTFTTEINVKDFNTKGKHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M6X3</accession>
    <name>A0A8S1M6X3_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myb-like DNA-binding domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0550110</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="85"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="144"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="144"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="205"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="210"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="543"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="602"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="602"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="663"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="668"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8075857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="979" mass="116221" checksum="D6F833483B0AF111" modified="2022-10-12" version="1">MQNSSRSLSSKKNEKLISKRNRSLQETESYGKFLEFFNSNQNQPFKQFLYMQEFFNTNLNSFPSDEEIQAIQQNLFSVYQVISNRKKNWTTDEKKVLIWVVGKLSQSRDLDIRDLPNSFWDDVSELICRRDPQSCKQKWLQLKKTDLQQKPFTQQEDQLLLDLIHKYKDSEQGKKWCQISEELNSQILNYRTSKQCRERWLNHLNPKISKDPWNDDEDIFLLDLILQQGRKWAEISKLFDGRRNENTVKNRFNSLIKREKDLIVDLQNGQTTNLDTILGNPTGQTLNKQQIMAIEVLKNKIQWRKGQIKSGEQINDFRRTSIHQTSEKQEEFQLKRSSVVKYQVGHLGQEEIQNDELVPCLVNLEKNIIYFCSKEQIVKLLNNQINQMNYNLELEKKDIKSFDSGLINFKTQLSIISEINDSKFSSFLCGQNNEDNFSQISKVFQNNFIQKQMIDLEQYFNLDPPQHQQINQLFNPIQQIHYKAYYQIRRAYKSEIKKFLGLCLMQFXMQEFFNTNLNSFPSDEEIQAIQQNLFSVYQVISNRKKNWTTDEKKVLIWVVGKLSQSRDLDIRDLPNSFWDDVSELICRRDPQSCKQKWLQLKKTDLQQKPFTQQEDQLLLDLIHKYKDSEQGKKWCQISEELNSQILNYRTSKQCRERWLNHLNPKISKDPWNDDEDIFLLDLILQQGRKWAEISKLFDGRRNENTVKNRFNSLIKREKDLIVDLQNGQTTNLDTILGNPTGQTLNKQQIMAIEVLKNKIQWRKGQIKSGEQINDFRRTSIHQTSEKQEEFQLKRSSVVKYQVGHLGQEEIQNDELVPCLVNLEKNIIYFCSKEQIVKLLNNQINQMNYNLELEKKDIKSFDSGLINFKTQLSIISEINDSKFSSFLCGQNNEDNFSQISKVFQNNFIQKQMIDLEQYFNLDPPQHQQINQLFNPIQQIHYKAYYQIRRAYKSEVLQQNFKIIDQKIPRSLPNVISIISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M774</accession>
    <name>A0A8S1M774_PARPR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPRIM_AZ9-3.1.T0550161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000055">
        <property type="protein sequence ID" value="CAD8075957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M774"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011893">
        <property type="entry name" value="Selenoprotein_Rdx-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044715">
        <property type="entry name" value="WDR86-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489:SF11">
        <property type="entry name" value="WD REPEAT DOMAIN 86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10262">
        <property type="entry name" value="Rdx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="233"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="414"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="828"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="436"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="836"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8075957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="847" mass="98438" checksum="87F237BC05406BF1" modified="2022-10-12" version="1">MKQTRVQSAATNSRLRDNHYDPKVHTKAWIAPGIFNQLPKYEHLDSGLVVINQKNNKMKQFKEFSELNQEIQQMQEKDEFRKFKEFENLYNGDLVVTIEYCTNCSQHAGSTRHDEEKYYTFATNLKQELIQQYPTAKVYLKPLIYDPHDYSIDTLYIQRRIGAFEVQVCSKNGDQFKKGLVFSKLNTKMWPNYTEVIERVSDYLNTAPLEIYVSFGENITGNLNNIEVTLTPHRQDRPQSSVSRISSQTKRTNRTTTKERKIKAKTNNQGYIKLQDVPVDVYVVEVQESNDYLNEQLLLNMIEMAEQNNDTIQVVIKLKKQTHSYLDIKIFNQQGSGLSEAKITITNLQTQESMLSREIESGRYEAIIEPNQYTLTVQKRGYKELSQNFDAVQGVNEFKLILEVDPNDQQLIKPPQSAKQLQPIQKPSSARPTSAQRSQQGSNTNQSQQQMNKQQQQQQQQQQQQQQQLPPKPRSQQQSIQSYEQPEYMEPTKQEAISNPNRPISGYKSKQVFIYDPYTNLPIEGVQVILNEESTKQAQTYVTDQEGTCRIIVQGVLEGKMVIQTEGYFPIVEEYGTTQSKMNLYQLRELSFPLIQHLEDKNSVMILVQTNVEVMPIDIKMILPDNVQIDQSHQNVLYDLNDESGCQRIIVHDLNTKRGVYRIIADIIEPDYVHPQHLKVYIITNKELKLVDVPKTINQEQIYWDIGVICAPNSGFLEINTPTDEILKRDKYLKDYQSLLQFIKNSKNMDLKTILGFNDKERLELQGDVFVQKDKIKATLDKYGFESITNLDYLIQSAMMSNGLYSFKRLEQKFGNLDFEFIFDDENSTSKQKEMSEDEPYDDDYDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1M8L8</accession>
    <name>A0A8S1M8L8_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPRIM_AZ9-3.1.T0470254</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000047">
        <property type="protein sequence ID" value="CAD8071584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1M8L8"/>
    <dbReference type="OMA" id="IFECTNQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="3">
        <location>
            <begin position="196"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="255"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="291"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="329"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="400"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="437"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="482"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8071584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000688137"/>
        </source>
    </evidence>
    <sequence length="548" mass="64079" checksum="7F25DA6A8A25C82C" modified="2022-10-12" version="1">MNNQNDDYLPSLLQEFQGTENAEGQLSEYQKVKLGNLGLMTPMSNTLTTDFEQMTLDTSPKESLVNQKNCLIIDTIDEQEENIFKLQVQGTINAQKHQSFKVPKEQKRKSPKHKTCQPLLTEPINQGIQENQDAILEMQSPSSNDTPSLNAQTLQLIKELWNSEKNESEYFQDIIDGHSIPQGLSYDYSYLNQPFYSPPLLESQSPQVKFEPDIEMDLYVTSLVDQLWGNQIVSRKLQKMLEQGTQEQKELITYKLERISPQIQKDVFGNYVVQKIFECTNQKLQLRMFNKLKSHFYDLSKNNFGCRVMQKLIEYTYNREDLQLIVLQQLQSNMRSLIYDLNGNYVIFKMLETYDKLKMEFLIPIVEESFNYMGQQIYGCKIIHKVIQQYTPQQISRIIRLSVQNYNILSQTEYGNYVLQHILQHWKPSQEKGYLVQLVIQQFYQLSVNKYASNTVERALEVLGKQELIAIMNWLLSRNPNQYSSNFVILANHQYANYVIKKFLVLSDHSVQKYISDHLQQNQSELTAIKSTVHGQRIYSLLEKSIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1MAK6</accession>
    <name>A0A8S1MAK6_PARPR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPRIM_AZ9-3.1.T0580059</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium primaurelia</name>
        <dbReference type="NCBI Taxonomy" id="5886"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDM010000059">
        <property type="protein sequence ID" value="CAD8077330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1MAK6"/>
    <dbReference type="OMA" id="DGCLNCQ"/>
    <dbReference type="Proteomes" id="UP000688137">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value=
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